LOCUS       DASJUF010000001        80105 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJUF010000001 DASJUF010000000
VERSION     DASJUF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.001"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(180..920)
                     /locus_tag="U4B62_000001"
     CDS             complement(180..920)
                     /locus_tag="U4B62_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140192.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1050..1997)
                     /locus_tag="U4B62_000002"
     CDS             complement(1050..1997)
                     /locus_tag="U4B62_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2140193.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2126..2890)
                     /locus_tag="U4B62_000003"
     CDS             complement(2126..2890)
                     /locus_tag="U4B62_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2140194.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2894..3283)
                     /locus_tag="U4B62_000004"
     CDS             complement(2894..3283)
                     /locus_tag="U4B62_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140195.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3418..3960
                     /locus_tag="U4B62_000005"
     CDS             3418..3960
                     /locus_tag="U4B62_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN2140196.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4108..4740
                     /locus_tag="U4B62_000006"
     CDS             4108..4740
                     /locus_tag="U4B62_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140197.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4874..4999)
                     /locus_tag="U4B62_000007"
     CDS             complement(4874..4999)
                     /locus_tag="U4B62_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2140198.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5133..6026)
                     /locus_tag="U4B62_000008"
     CDS             complement(5133..6026)
                     /locus_tag="U4B62_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN2140199.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6208..7134)
                     /locus_tag="U4B62_000009"
     CDS             complement(6208..7134)
                     /locus_tag="U4B62_000009"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN2140200.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7219..7980)
                     /gene="pflA"
                     /locus_tag="U4B62_000010"
     CDS             complement(7219..7980)
                     /gene="pflA"
                     /locus_tag="U4B62_000010"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN2140201.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8224..10455)
                     /gene="pflB"
                     /locus_tag="U4B62_000011"
     CDS             complement(8224..10455)
                     /gene="pflB"
                     /locus_tag="U4B62_000011"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN2140202.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10732..13182)
                     /gene="parC"
                     /locus_tag="U4B62_000012"
     CDS             complement(10732..13182)
                     /gene="parC"
                     /locus_tag="U4B62_000012"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN2140203.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13194..15239)
                     /gene="parE"
                     /locus_tag="U4B62_000013"
     CDS             complement(13194..15239)
                     /gene="parE"
                     /locus_tag="U4B62_000013"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN2140204.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15405..15839)
                     /locus_tag="U4B62_000014"
     CDS             complement(15405..15839)
                     /locus_tag="U4B62_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN2140205.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15966..16604
                     /gene="plsY"
                     /locus_tag="U4B62_000015"
     CDS             15966..16604
                     /gene="plsY"
                     /locus_tag="U4B62_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN2140206.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16736..17611)
                     /locus_tag="U4B62_000016"
     CDS             complement(16736..17611)
                     /locus_tag="U4B62_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN2140207.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17693..18484)
                     /gene="codY"
                     /locus_tag="U4B62_000017"
     CDS             complement(17693..18484)
                     /gene="codY"
                     /locus_tag="U4B62_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN2140208.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18502..19902)
                     /gene="hslU"
                     /locus_tag="U4B62_000018"
     CDS             complement(18502..19902)
                     /gene="hslU"
                     /locus_tag="U4B62_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN2140209.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19916..20464)
                     /gene="hslV"
                     /locus_tag="U4B62_000019"
     CDS             complement(19916..20464)
                     /gene="hslV"
                     /locus_tag="U4B62_000019"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN2140210.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20501..21406)
                     /gene="xerC"
                     /locus_tag="U4B62_000020"
     CDS             complement(20501..21406)
                     /gene="xerC"
                     /locus_tag="U4B62_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN2140211.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21604..22980)
                     /gene="trmFO"
                     /locus_tag="U4B62_000021"
     CDS             complement(21604..22980)
                     /gene="trmFO"
                     /locus_tag="U4B62_000021"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN2140212.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22949..25027)
                     /gene="topA"
                     /locus_tag="U4B62_000022"
     CDS             complement(22949..25027)
                     /gene="topA"
                     /locus_tag="U4B62_000022"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN2140213.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25149..26006)
                     /gene="dprA"
                     /locus_tag="U4B62_000023"
     CDS             complement(25149..26006)
                     /gene="dprA"
                     /locus_tag="U4B62_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN2140214.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26062..26829)
                     /locus_tag="U4B62_000024"
     CDS             complement(26062..26829)
                     /locus_tag="U4B62_000024"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN2140215.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26822..27682)
                     /gene="ylqF"
                     /locus_tag="U4B62_000025"
     CDS             complement(26822..27682)
                     /gene="ylqF"
                     /locus_tag="U4B62_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN2140216.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27942..28340
                     /locus_tag="U4B62_000026"
     CDS             27942..28340
                     /locus_tag="U4B62_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2140217.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28619..29119
                     /locus_tag="U4B62_000027"
     CDS             28619..29119
                     /locus_tag="U4B62_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2140218.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(29202..29753)
                     /gene="lepB"
                     /locus_tag="U4B62_000028"
     CDS             complement(29202..29753)
                     /gene="lepB"
                     /locus_tag="U4B62_000028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2140219.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29905..30132)
                     /locus_tag="U4B62_000029"
     CDS             complement(29905..30132)
                     /locus_tag="U4B62_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN2140220.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30129..30647)
                     /gene="msrA"
                     /locus_tag="U4B62_000030"
     CDS             complement(30129..30647)
                     /gene="msrA"
                     /locus_tag="U4B62_000030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2140221.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30667..31380)
                     /locus_tag="U4B62_000031"
     CDS             complement(30667..31380)
                     /locus_tag="U4B62_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN2140222.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31383..32279)
                     /locus_tag="U4B62_000032"
     CDS             complement(31383..32279)
                     /locus_tag="U4B62_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2140223.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32437..33279)
                     /locus_tag="U4B62_000033"
     CDS             complement(32437..33279)
                     /locus_tag="U4B62_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2140224.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33411..34064
                     /locus_tag="U4B62_000034"
     CDS             33411..34064
                     /locus_tag="U4B62_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN2140225.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34127..34654)
                     /locus_tag="U4B62_000035"
     CDS             complement(34127..34654)
                     /locus_tag="U4B62_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2140226.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34674..35621)
                     /locus_tag="U4B62_000036"
     CDS             complement(34674..35621)
                     /locus_tag="U4B62_000036"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN2140227.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35633..37519)
                     /locus_tag="U4B62_000037"
     CDS             complement(35633..37519)
                     /locus_tag="U4B62_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2140228.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(37679..39757)
                     /locus_tag="U4B62_000038"
     CDS             complement(37679..39757)
                     /locus_tag="U4B62_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN2140229.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39770..40045)
                     /locus_tag="U4B62_000039"
     CDS             complement(39770..40045)
                     /locus_tag="U4B62_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140230.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40407..40862)
                     /locus_tag="U4B62_000040"
     CDS             complement(40407..40862)
                     /locus_tag="U4B62_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140231.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(41016..42224)
                     /locus_tag="U4B62_000041"
     CDS             complement(41016..42224)
                     /locus_tag="U4B62_000041"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN2140232.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42336..42668)
                     /locus_tag="U4B62_000042"
     CDS             complement(42336..42668)
                     /locus_tag="U4B62_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2140233.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42816..43691
                     /locus_tag="U4B62_000043"
     CDS             42816..43691
                     /locus_tag="U4B62_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2140234.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43773..44354)
                     /locus_tag="U4B62_000044"
     CDS             complement(43773..44354)
                     /locus_tag="U4B62_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN2140235.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44360..45619)
                     /locus_tag="U4B62_000045"
     CDS             complement(44360..45619)
                     /locus_tag="U4B62_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2140236.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45621..46667)
                     /locus_tag="U4B62_000046"
     CDS             complement(45621..46667)
                     /locus_tag="U4B62_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140237.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46902..47177)
                     /locus_tag="U4B62_000047"
     CDS             complement(46902..47177)
                     /locus_tag="U4B62_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN2140238.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47420..48730)
                     /gene="der"
                     /locus_tag="U4B62_000048"
     CDS             complement(47420..48730)
                     /gene="der"
                     /locus_tag="U4B62_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN2140239.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48912..50141)
                     /gene="rpsA"
                     /locus_tag="U4B62_000049"
     CDS             complement(48912..50141)
                     /gene="rpsA"
                     /locus_tag="U4B62_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN2140240.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50276..50956)
                     /locus_tag="U4B62_000050"
     CDS             complement(50276..50956)
                     /locus_tag="U4B62_000050"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN2140241.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(51005..51640)
                     /locus_tag="U4B62_000051"
     CDS             complement(51005..51640)
                     /locus_tag="U4B62_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2140242.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51706..53109)
                     /locus_tag="U4B62_000052"
     CDS             complement(51706..53109)
                     /locus_tag="U4B62_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN2140243.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(53096..54127)
                     /locus_tag="U4B62_000053"
     CDS             complement(53096..54127)
                     /locus_tag="U4B62_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2140244.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54158..54379
                     /locus_tag="U4B62_000054"
     CDS             54158..54379
                     /locus_tag="U4B62_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN2140245.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54544..55281)
                     /locus_tag="U4B62_000055"
     CDS             complement(54544..55281)
                     /locus_tag="U4B62_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN2140246.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55434..56774)
                     /locus_tag="U4B62_000056"
     CDS             complement(55434..56774)
                     /locus_tag="U4B62_000056"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN2140247.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(57011..58534)
                     /locus_tag="U4B62_000057"
     CDS             complement(57011..58534)
                     /locus_tag="U4B62_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN2140248.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58912..59523)
                     /locus_tag="U4B62_000058"
     CDS             complement(58912..59523)
                     /locus_tag="U4B62_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2140249.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(59646..59771)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(59853..60569)
                     /locus_tag="U4B62_000059"
     CDS             complement(59853..60569)
                     /locus_tag="U4B62_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2140250.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(60575..61144)
                     /gene="scpB"
                     /locus_tag="U4B62_000060"
     CDS             complement(60575..61144)
                     /gene="scpB"
                     /locus_tag="U4B62_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN2140251.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(61128..61925)
                     /locus_tag="U4B62_000061"
     CDS             complement(61128..61925)
                     /locus_tag="U4B62_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN2140252.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(61895..62266)
                     /locus_tag="U4B62_000062"
     CDS             complement(61895..62266)
                     /locus_tag="U4B62_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2140253.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(62285..63172)
                     /gene="xerD"
                     /locus_tag="U4B62_000063"
     CDS             complement(62285..63172)
                     /gene="xerD"
                     /locus_tag="U4B62_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN2140254.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(63268..63741)
                     /locus_tag="U4B62_000064"
     CDS             complement(63268..63741)
                     /locus_tag="U4B62_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2140255.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(63846..64709)
                     /locus_tag="U4B62_000065"
     CDS             complement(63846..64709)
                     /locus_tag="U4B62_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2140256.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(64905..67004)
                     /locus_tag="U4B62_000066"
     CDS             complement(64905..67004)
                     /locus_tag="U4B62_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2140257.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(67224..68333)
                     /gene="rpoD"
                     /locus_tag="U4B62_000067"
     CDS             complement(67224..68333)
                     /gene="rpoD"
                     /locus_tag="U4B62_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN2140258.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(68357..70198)
                     /locus_tag="U4B62_000068"
     CDS             complement(68357..70198)
                     /locus_tag="U4B62_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN2140259.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(70380..72695)
                     /locus_tag="U4B62_000069"
     CDS             complement(70380..72695)
                     /locus_tag="U4B62_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2140260.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(72880..73740)
                     /locus_tag="U4B62_000070"
     CDS             complement(72880..73740)
                     /locus_tag="U4B62_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN2140261.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(73799..74320)
                     /locus_tag="U4B62_000071"
     CDS             complement(73799..74320)
                     /locus_tag="U4B62_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN2140262.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            74598..75359
                     /locus_tag="U4B62_000072"
     CDS             74598..75359
                     /locus_tag="U4B62_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2140263.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            75385..77976
                     /gene="mprF"
                     /locus_tag="U4B62_000073"
     CDS             75385..77976
                     /gene="mprF"
                     /locus_tag="U4B62_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2140264.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(78164..79351)
                     /gene="tuf"
                     /locus_tag="U4B62_000074"
     CDS             complement(78164..79351)
                     /gene="tuf"
                     /locus_tag="U4B62_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2140265.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            79719..80075
                     /locus_tag="U4B62_000075"
     CDS             79719..80075
                     /locus_tag="U4B62_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2140266.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        21962 a        16841 c        13739 g        27563 t
ORIGIN      
        1 tctgcgaaaa tagtttgttt ttttattttg ccattatttt cttacttatt gggagaaaaa
       61 attcgcttaa actcaaaaaa atttgaggat acgatataaa ctttcggata gagtataaaa
      121 taaggaaacg ccgcagcgtt tcctttccaa ctgtagccga atataaattt gtatatcact
      181 tatttaaaat agctattttc tcccaaagag tttccttgat catcgatcaa tccttgtaat
      241 gagcgttcta agataccatg gctttttttc gatttttttt cttctgactg ctttgtttcc
      301 tctaacgttt ttgttcgatt gaatgttcgt acagacggct caccatttct tttttcttga
      361 tacggcgtcg gttctgtatc gaacaacaga taagagtcat ccgttttttt catcgattcg
      421 attaaatgat cctcagaaat tttgctttct ctagggtctg ggataatcga tgcgggtaca
      481 tatttaggaa caaaataaga acgctcccgc accttcgtgt caacagatgc gtttcgtgta
      541 gtatgcttgg actctgtcct tgtttcactt tgtctgacag aacgagagga atcaccaaaa
      601 agtttttttc gtcctgtcga tgtgatttct gattggtaat ggccggtata atccggtaat
      661 tttcctttat gcttttccaa attttcctta cgcggagctg acttcaatcc atttgttgca
      721 cgacgcttgc gacggatttt ctcggtgttc gtgttcgtga aatgaaattc tttcatctgt
      781 tcaaacgggg actgattctg ttcagtcaaa agatcttctt gtccgtcaaa taatactcgc
      841 ttcttgtcgt tggttaattt tacgcctaac tcatcatcgt acgcaggaaa acggaaatgt
      901 tttcgtctga tttctttcat tcaaaccatc cttctcaaaa atggaacttt tctcaataat
      961 agcacagatt cattgttttc agtagcaatg cgctgattat tttaagaaaa tagcgatgaa
     1021 tttcataaat atatatatag taataagact taattcgttg tctctctgat aactagttca
     1081 ggaaaaaaac gacgataacc ttgactcgcc cctttttctt ctattttttt taacaacatt
     1141 tctccacata cttcacccat acgtataata tcttgcttga tcgttgtcaa tttaggtgaa
     1201 ctgatttggt ctaaaaatac cccatcaaat ccaatcacac caaattcttc gggcaagtgt
     1261 cctccagcat ttataatccc tcttactgct cctagtgcta gccgatctga actacataca
     1321 aaacaagttt tatctgtaaa atctgcccag ttttctgtca tatatctttc tacttctgtc
     1381 gaatgatttg gaaaccgcaa gattttacta ggaagatcaa cttccttcat cacagataaa
     1441 taaccagttt cccttgaaat ttcaaaaggc tcgtccactt cgataccgat ataaataatc
     1501 gtttgataat ctctttctaa ggcatattgc gttgcttttg ctgtaccata ttcattatcg
     1561 ctgtctacat aatcatatcc ttcagaattt tccccaaaca agacaactgg tttttccaaa
     1621 cggtcgatcc aatcgaagtc tttttttctg acacctgtga tgatgtatcc atcacaagaa
     1681 ccgacgtcga aggcatcatt tgtaactaat tggagagaat aatgcgcttt accgatccgt
     1741 ttagcgattc ccatcagcaa attcatgtaa tagggctcag ttgtgtcgat ttcttccagt
     1801 ataaataatt tgatgatctg cgaccgattc gtgacaagtg cttttgcagc aatatttgga
     1861 cggtaatcca gttcagccat tgctgagaaa actaactctt tcaattcgtc agtcacctgt
     1921 tccggatgat tgatgactcg agagaccgtc atttttgaaa cattggcacg ttttgcaaca
     1981 tctgctaaag tgggcatgct ctcttccttt ctaacatgtt gtcattctta ctatcaatta
     2041 taccaaattt tcctaacaga gtttattata gcccaaaata aaaaacggaa aatcctctat
     2101 cgtcatgagt gattttccgt ttcaattatt tatacttttt tatcaaattt tttgctaaaa
     2161 aagtggaagc ttcttccatt agtggtattt gtaccatatg tcgctgtgtt tttgaggaac
     2221 gaaaaacaat ttcttttccg taagacttat gccgattttt ttctacaaat cgttccgttt
     2281 gtatataagg gattttttcg tcagcttgtc catgccaaaa gaacagagga cgtccagcta
     2341 acttttccgg ctgcaatgac aaatcatagc tcttaaccca tgaaatcaaa tcaaaatagt
     2401 cttttggtac gacataatta tattccgatg cgcgccttac gatttctttt ccatattcca
     2461 aaggtgcagg ggaacccatc aaacacacgc ccgctactat atgaggatgt ttagccaaca
     2521 aggcgcatgt tgtcattccc cccatcgaaa ccccgccaac tgcaagctga tcatgtagaa
     2581 gacctctttt ttctaaatgc ttgataagtg tgtcaaattc aaacagattt ccgtagatac
     2641 tatcccaaaa tgtataggat ggaatagaag aaaccggctg tttgcgctcc ccatgattca
     2701 tcgcatctgg caggatcaca cgaatgccct gctgcgcaat ttttcttcct tgcgtcaaaa
     2761 ctaactcctt acttgtctgc catccgtgat aatagatgac aacgggaata cgttcctctc
     2821 ttaattcttt cagaacaact tctaacactg gaatatgatg gacagaacgt ttcaatataa
     2881 ttggattcaa ttgttactca cctattttca ttaaatttcc tgatcgccat agcaaccatg
     2941 ctccaacagt tgtctggaac aggttcagtg taccaaataa tataaatgca ggactgaatg
     3001 gctcgggaaa taagcacaga gaacaaagtc ccaccactcc taatgctagc gaaaggagtg
     3061 gataaataga aaaattcaaa aaactggcaa cgctaccgaa aatattacca ataaacagta
     3121 tcaaaaagaa aaaaacgctt aaaattaaac catctgtcag cgacatctga aaaacaatgt
     3181 tctctacctc aattggctgt tgaatcgaat agacacctaa gtatatactg agaaggagac
     3241 ttagcacgtg gctggcacaa aacacacgat tgaccgtttt cattttaatc acctaaccta
     3301 tctttataat tctattataa tcattttatc atttttttgt atgtataaag tttcaatttt
     3361 tcttcactta cgattttttt tgatattgtt gttatataaa tgaaaggagt tttacaaatg
     3421 attgatccaa aaatgttggg gatgatcttc ctcattaatt ttgcttatat cactctgaac
     3481 acgttacgtt tcatgttgac aatgaaaggc tatcgactga ttgctccact tgtcagtatg
     3541 gtcgagatca cgatttatat cgtcggctta tcccttgttt tagatcgttt agataatcca
     3601 ctgaatttat ttgtctatgc gttaggttac gcagtaggta tcagtgtcgg aattttgatt
     3661 gaagacaaat tggcattagg atacatcatg gtcacaacga ttttgccagc aaatagcgca
     3721 gaagataaaa gtttgccggg tatactgaga gaacacggtt atggtgtgac acagacttct
     3781 gcaatggggc tggaaggtga gcggctagtt ctcgaaatac tttctcctag aaaaagcgaa
     3841 cgtgaactgt atcgcctgat caaagaaatc gaagagcgtg cttttatcat ttcttatgag
     3901 ccaaaatata tttcaggtgg tttctggact aaaaaagtga gaaaacgaca aggaaaataa
     3961 ctcacaaact taaaaactgt ctaatatcta aattaaattt taaatgttag acagttttta
     4021 tttattattt tgattaaaaa acatcgacat atactcatca atttgttatt atactttata
     4081 tacttatcaa tttaatttgg agatattatg gaaattcaac gtagaaccac taatttttta
     4141 catttaggct ctttttttgc aactttgacg atcttatttt ttctatgtta ttcatacctt
     4201 aagacctcac atgcgccttc tagtgcatta ggctggttgc ttttggatca ttctgatatc
     4261 acagaagccg aagaaaatcc gttttttttg attttgtctt ctctttgcgg cttactgttt
     4321 tctgtttatt tcggtgccta tttttttcat cgacatcctg gtacgttaaa cgaagcttca
     4381 agcgtattga ttgcatttaa aacgttttct tttttagcaa ttctttttta ttcgtttaat
     4441 cacaatacgc ttttcttctt tatcatgaat ggtttagtcg tgattttatc catttttact
     4501 tattttattt tttcactcat ttttagccaa ttgaaatgtc cgctcttctt tcgattagtt
     4561 cctgtcacct gttttattta cagcttgttt tccttttttg tccttgcttt aatgccactg
     4621 accaaccctt cgacgatcca agtcgcagga aatctgttgg atatgctctt catcgtcagt
     4681 ctaggtgtat ttatgtggca aagaaaaaac aaacaaaata aaaagcattt tgaagcctga
     4741 ctattacgac ttcataaatg cttttttagt tttaaacact tcaatctaaa agaataaccc
     4801 aagattggat aaattattaa tgactcgcca ttcgtttctt agtgattctg gattatcaaa
     4861 tggacttcct aatctaatca gctagttctt cccataaaat gccatacttg actgaatcat
     4921 aatattgatt atttaaaaat ctaacttttc ttatcactac ttcctttgtc attccacatt
     4981 tctcaccatt tttttgcatt cctttgtttc cagaccatgt agtgaatcta ataggtggta
     5041 gataggcaaa atactcaaat aattctttta accataaagg aagagaaagg ctcctcctgc
     5101 aagagtcagt ataatttttg cttactgctc tcttaggttt tactcactaa aactgttcca
     5161 tattctttga tataagaaaa tgcatgcggt ataaatttct tgctcagctc agaaaagatt
     5221 tgtcgtttaa gaaaggctat ttcctccatt gtcaatgaag gatccaatgt tggtaattca
     5281 atctcaaaat tgtttttttc gacaataggt aattctaatg tgaatgattc ttcttccttt
     5341 atagtattcg tcacaagtag agactgatgg aaaatactag gccggcgatt tttatattta
     5401 ttcttacgta tcctgtcaac gataacttct atttgatcaa aaaagtacga ttcatttacg
     5461 tctccaataa gttcggctaa ttctttgaat gccttcgtta caggaagctg attcttctgt
     5521 acaaaaaagt aattagccaa agtaagcggg aagtgttcta tagaggaaag tgtgataaaa
     5581 cggtacttct catttcctgc agttgtcctc agcaattctg tctctcggct gtttcttact
     5641 ccataaaaat acgcttcttt ccgttcatct tcgaaacaat acgcccaaat aacttctccc
     5701 atcgctaatt tttgttcatc aacagagagt ctcttcaatt gttcagctat ggtttcagca
     5761 gccgaagtgg cttgttgcaa gtaatcttta ggatcaaata ttggaaagtt caaatgatat
     5821 cttcgttctt ccctcttgat taatcctgca tcgatcaata aatcaagaaa atgggaaaat
     5881 ttattttgcg aaaattcttt tttcaattct cttagaatcg tttcttgttc tcgatgttca
     5941 tatagataat cggtcaatga agaaaaaagc ggatcatcgg tcagtttttg ataaaagttt
     6001 tccttatttt ttgaataata gttcatatat gttctctttt ctatccgttt tattttactt
     6061 aaaaggcttt tacgtaaaaa ggaggaagaa caagagtcat aattgactgt tattctcctc
     6121 catttcttcg gcctttagta tgttctatgt ttctttttat atcaatcgct tatccattca
     6181 ttttgaatgc ttcatactac catttgatta gctaaataca gccgtcaatt gaggtacgac
     6241 ttgtttttta cgtgaaacga cacctttaag caaagcacgg ttgttggtca aagttgtttg
     6301 gaaagcttct tctacttttt cttttccttt accagcaaca agtaattctg aatcactgtc
     6361 taagatgttt gtgacaacta atacgaacag atcgtagcca tttgctttca tttcatcttc
     6421 cattgctgct tctagctctg cttgtcgatc catcacttca ttaaggtcca ctgtattgac
     6481 ttgagcgata cgaacgttgg aactgcccat tgggaaactt tttgcatcca aatcaagaag
     6541 aaccgcagct gatttatcac ttagatttgt tcctgctttc aacatttcaa gtccataagt
     6601 gtttagatcg acttcagcga ttgctgcaag ttcttttgca gctgcttcat cttcaggcgt
     6661 acatgttggt gatttgaaca acaatgtatc tgaaatgata gctgagagca tgattcctgc
     6721 gatttcttta ggaattgtta cttggttttc tttatatagt tttaaaataa tcgtacttgt
     6781 acatccaact ggttctgcac gatagtacaa tggattagct gtttcaaagt ttgcaatacg
     6841 atggtggtct actacagcta aaatatttac ttcgctgata tctgaaatac tttgttggaa
     6901 ttcattatga tccacaagca tgacatctgc attttctgct ttagctgact ctactactcg
     6961 tggtgcagat agctgaaagt ggtctaatgc gtattgtgtt tcttcattag gtgtacctag
     7021 agcaactgct tctgtttctt tacctaattc attttgcaag tgtgaaaacg caatggctgc
     7081 cccgattgca tctgtatctg gattttggtg accaaaaact aaaatttttg acatgtcatt
     7141 cgctcctttg tgatttctcc tatttaataa tagcaaaaaa acggaaagaa gcaatactta
     7201 tcttctttcc gatgcgacct atcgcgttaa atattttgta tactcttctg tatgaagcaa
     7261 tcgtttagca ttttcgacac gttcttttgt aggtggttcg atcccttcta atggatattt
     7321 caaaccaagt gtttcccact tgtattttcc catcgtatga taaggaagaa tctctactct
     7381 gtctacattt gacaaggttt gaataaagtt gtctagtcgg atcaaatatt catcataatc
     7441 acttcgctca ggaactagta catggcgaat ccaaaccggt ttgttgattt ttgacaaata
     7501 ttgagccatt tctaaaatat tttcattgtc ccaatgcgtt agttttttat gctcttcatt
     7561 atcgatgtgt ttgatgtcaa ataacagtaa atctgtatac ttcatcaatt cttgaaaacg
     7621 tgaaaagaaa ggttcttcat acgtaaaagg ttttccgcat gtatccaatg tagtatgcat
     7681 cttccgctct tttgcttttt taaatagttc gatcaaaaaa tcgatctgca atagaggttc
     7741 tccaccacta acagtaatac cacctttatc tccccagtaa ggtctgaagc gttctgcttt
     7801 atctaaaagt tcatctgctg tgtattcttt ccctccgcca atattccaag tatctggatt
     7861 gtgacaaaat tggcaacgca tgcggcatcc ttgcataaac acgacaaagc gcagtccagg
     7921 tccgtctact gaaccgaaag tttcgataga atgaacatag ccgattgttt tttcttccat
     7981 ttaagttcac atcttttctt tgatttcatt ttaagtacaa gttagctgtc gaagcgaagc
     8041 tttctgaaca aaatttttcg caattgccgc ttcgttatgt gtaaaattta cagggacgga
     8101 acatacaagt cctgcccctg taaatcgttt ctttcaaata agcatactga ttattcgatt
     8161 ttgatgcgat ggctattcaa cgtcctttcg acgctaagtc gaaaaacttt tttcacgaac
     8221 tttttacatt ctgtcgtgtg aagttctcat caagacatct aattgttgtt ctcttgtcaa
     8281 gtcacggaat ttaactgcat aaccagatac ccggatcgtc aagttcggat atttcattgg
     8341 attttccatt gcatcacgca ataaatcatt actgaataca ttgacattca aatgataagc
     8401 tccttttgag aagtatccat ctaagacatt acgcaagttt tcgattcttg tatcttgttc
     8461 ttttccaaga cctgaaggat tgattgtttg ggtatttgaa attccatcca atgcacagcc
     8521 gtaattcatt ttagctgttg agttcaggct agctaataac ccactttgtt ccgcattgta
     8581 acttggattt gctcctggcg caagtggttt tccagcttca cggccatcag gagtatttcc
     8641 tgtattttta ccgtaaacaa catttgaagt aatcgtcaat aatgaagttg ttggttttga
     8701 gttgcggtaa gttttatgac gtttgatttg tcccatgaag tattccaaga tccatgaaac
     8761 taattcatct gcacggtcat catcatttcc gtatttaggg aagtcgccat ccacttcaaa
     8821 atcaactgct attccttttt cattacggat tgcttttact tttgcatatt tgatcgctga
     8881 caagctgtct gccgcatgag agatcccagc aatacctgta gcaaatgtgc gttgcaagtc
     8941 agaatccatc aaagccagct gagctgcttc ataagaatat ttatcatgca tataatgaat
     9001 gatgttcaac gtattgacat atagttctgc taaccaatcc atcatattca tgaagttatg
     9061 tttcacttct tcaaagtcta gtatttctga tgtgattgga cgatattctg gtccaacttg
     9121 ggcgccagtc acttcatcga ctccgccatt gatggcatat aatagtgttt tagccaagtt
     9181 agcacgagcg ccaaagaatt gcatgtcttt tccgacaact gttcctgata cacagcaagc
     9241 aatcgcacaa tcatcagaac cccattgttc acgaagctga ttatcatttt cgaattgaat
     9301 cgaagaactt tcgatagcga ttctgctagc atatgttctg aatccttctg gaaggtgttc
     9361 agaatacaaa acagtcaagt ttggttctgg tgaaggtccc atatttgtta atgtatgaag
     9421 cagacggaaa tcattctttg taactaatga acgaccatct aatcccatac ctgcaatgga
     9481 taaagtagcc cagataggga atcctgagaa taattggtta tattcaggtg tacgcgcgaa
     9541 tttgaccata cgtaatttca ttactaagtg gtcgatcaat tcttgtgctt ctttttctgt
     9601 gattactcca ttatccaaat cacgttggat atagatatca aggaatgctg agatacgtcc
     9661 gattgacatc gcagcaccat tttgtgattt gattgctgct aaatatccga agtatagcca
     9721 ttggacagct tcttttgcat ttgcagctgg tttagaaata tcatagccat atttcgcagc
     9781 catttctttc atttgcgcta atgcacgata ttgttcagtg atttcttcac gtaaacgaat
     9841 cacttcttca ctcattgtat gataaccagt catatcatga tcttttttct tttgttccat
     9901 caaatagtcg attccataca atgccacacg acgatagtca ccgatgatac gtccgcgacc
     9961 gtaagcgtct ggaagccctg taatgatttt gtttttacga gcagcacgca tttcagcagt
    10021 ataagcgtca aatacacctt gattatgtgt tttgcgccag tcagtgaaaa tatgtgtcaa
    10081 ttcatcgttt ggtgtatagc cattgctttt tagcgcattg ttagccatat tgattccgcc
    10141 gaacggcatg aatgcttgtt tcaaaggttt gtctgtctgt agaccgacga ttttttccaa
    10201 atctttatcc aaatagcctg catcgtgtga agtaatagtt gatacgattt ctgtgtccat
    10261 atcatagaca ccgttacgct cgaattggat atcatttaac ttttgcaatt catcccataa
    10321 tttttctgtt gcttctgttg ggccttccaa aaagctgtca tcaccgcgat attcagtgta
    10381 gttattttga ataaagtctc tagtgtctac gccttctttc catttggtac ctttaaatcc
    10441 attccattgc tccattgtat ggacctccta aatttctgtt ttatgtaaca gtttttacgt
    10501 gattagtata acacagcacc ctatctttgc aagggttttc ttgtttttta tttaacaatt
    10561 tttatgtttt atttacatat atttaaatca acgttgacat accaacattt aagcattgtg
    10621 aacactttca tctttttttg caaacgtata aggatttatt aaaaatgaat gaaaaagaga
    10681 cgccacctgt attaatacag atggcgtctc ttcattaaaa tactacaaca gttacgactc
    10741 gatgaaaatc tcaggagcat cgataacttc gatgacttct ccaccttttt tctcatccat
    10801 cgcaaatgat ccattcgatg ttcgatcacc tatagggaaa tcagttgggt caatcgttaa
    10861 ctgctgccct ttttgattca tcaaggttat ttcttttgat cttccttccg acatatagat
    10921 aatacggtgc ggattttttt tcagctcacg catgaccgtc actccccgct tcgcacgtga
    10981 agtttggctg atttcttgag caagcatgcg tttaacagct cctcgctgag taacgattac
    11041 gatcggcgta tcaccttctg ctaacacaag cagcccgttg ataatcctat ctccatcttt
    11101 caaattcata gacttcactc cagctgcctt tgctccaaca acgggaacat ctgttaaagg
    11161 atagcgcaaa cccattcctt gatgcgttac taagaaaaca tctaagcctg gctgatcttc
    11221 tttcagatac acagcaatca gttcatcttc ttccgccttt agcttcatac agctgagcgg
    11281 acggctctta taggtgcgcc atggttcgaa ctctgtcatt cgtgtttgct tgatcaatcc
    11341 attcttgctg atgaatacaa atgttttctc tgcttgaatt ttttgatatg ggaaaacagc
    11401 taaaatcgat tcgtctacag acagattgga gattgtttga gaaatgtgct caccagcatc
    11461 tttccatttc aaatcgggta gttcatggac agggcggtag atcacatttg ctttatttgt
    11521 gatcagaaga acatggtcca atgtactcaa ttcacctgag tatagcaagt agtcgccttc
    11581 tcgcatcccg atttcttctg gtttggaagc actataagaa cgaaggcttg ttcgcttcac
    11641 atagccttct cgcgtcacac tgacaattac gtcttcttgt gccactaaga cttgcgtatc
    11701 aattttgatt tcttcgattt cgtcttcgat tgtcgttaga cgagcggaag aataattttt
    11761 tttcacttct cgcaattctt ttttcataac agagaaaagc tctttgtcat ttgaaaggat
    11821 tttattcaac tctgtgattt gcgcaatcaa gctttcggat tccttttgaa gctctgtgat
    11881 atccgtgttc gtcaaacggt ataattgcaa tgtgacaatt gcttctgctt gttcttctgt
    11941 aaattgaaag acgtcgacta aattcttttt ggcgtctttt ttgtctttac tttcacgaat
    12001 cgttgcgatt acttcatcca gaatcgataa agctttcatc aatccttcta cgatatgctg
    12061 tcttttttga gctttggcta aatcgaattg actacgtttt gttatgactt gctttcgatg
    12121 ctcgatataa cttcttaaaa tgtctttcag tccaacttga tgcggagtca tatggtcgat
    12181 agcgaccata ttgaaattgt aattgatctg tagctcagtg tttttgaaca aataattcaa
    12241 aattccttga gcattggcgt cttttttcaa ttcgacgaca atttgcagac ctgttctatc
    12301 actttcatct cgtacttcag ctattccatc gattttctta ttcaaacgga tctcatccat
    12361 ttttttcacc aaggtcgctt tatttacttc atatgggatt tctgtgatca cgatttgctg
    12421 tttattaccc ttcattgcct ctatctttgt tttcgaacga agaataactt ttcctcgtcc
    12481 tgtttcataa gcttttttga tttcatcttt tccttgcaag atccctcctg ttggaaagtc
    12541 aggtccagga atatattcca tcaatttttc aaggctcgct tgtggatgat cgatcatata
    12601 gattgtgcca tcgatcactt cagctaagtt atgagtcggg atttctgttg catatccagc
    12661 agatatacct gtcgatccat tgacgagaag gtttgggtat cgagctggaa ggactgttgg
    12721 ttctttttct gtatcatcaa aattccacac gagatcgact gtttctttct cgatatctgc
    12781 tagcatctct ccacttaact tggataatct ggcttcagta taacgcatcg cagcaggtgg
    12841 atcaccgtcc atactcccgt tattcccgtg catttcgatc agtacttccc gcaacttcca
    12901 gtcttggctc atgcggacca tcgcttcata aatactgatg tcaccatgag gatgataatt
    12961 ccccatgata tttccgacag attttgctga tttacggaat cctttgtcat acgtattgcc
    13021 atctttgttc atggcaaata atatccggcg ttgcactggc ttcaatccat cacggatatc
    13081 aggcagtgca cgttcttgga taatatattt ggaataacgt ccgaagcggt cacccatcac
    13141 ttcttctaaa gttaattctt gtacttcttg tcgattttcc atcaggtgac ctcctattct
    13201 acttcaaata actgattgtt ttcattttta tctgctcgtt cttcatctaa taaatcatta
    13261 gaaactgaag gagagatttc agtatcctct tttttatcta agatactgcc atcttcttcg
    13321 agcgtaaatt gcacgtgatt ttcgatccat tttcttcgag gttctacttt gtcacccatc
    13381 agtgtggtta ctcgacgttc agcttgcgca gcatcatcga tacggacgcg gatcaacgta
    13441 cgggaagtcg gatccatcgt tgtttcccac aattgttccg cattcatctc tccaagtcct
    13501 ttatatcgct gcagcatata tcctttgccg acttttttaa tcattgctgc caattcgtca
    13561 tctgtccacg catattcaat gacttgcttc tttccttgtc ctttggatac tttatataaa
    13621 ggcggtaacg cgatatatac tttcccagct tcaatcagcg gtttcatata acgataaaag
    13681 aaagtaagca gtaatacttg aatatgggcg ccgtctgtat ccgcatcggt catgatgatt
    13741 actttgtcgt aattacagtc ttcgatagaa aactcaggac cgactcctgc accaatcgta
    13801 tagatcatcg tattgatttc ttcatttttc aagatatctt gcattttagc tttttcggta
    13861 ttgatgactt ttcctcgtaa aggtagaatt gcttggaact ttctgtctct tccttgttta
    13921 gcagatccgc cagcagaatc cccttcgact aaatacaatt catttttctt agggtttcgg
    13981 gattgtgcgg gtgtcaattt tcctgaaaga agagattcgc cttttttacg cttcttccca
    14041 tttcggcttt cttctctggc tttacgtgct gcttctctcg cttctctggc tttgattgct
    14101 ttacggacca acatctggct catttcgcta ttttcctgca aataaaagcc catttgttca
    14161 ctgatgacgt tatctactac ggtacgagca actggcgtac caagtttttc ttttgtttgc
    14221 ccttcaaatt gcagaagatt ttcaggtaca cggatagaaa gtacagcagc taacccttct
    14281 ctgaaatcac taccttccag attcttgtcc cgttctttca gcaaaccaac ttttctcgca
    14341 tattcattat aagatttcgt catcgccgct ttcataccag cttcatgggt tcccccatct
    14401 tttgtccgaa cgttattgac aaaagataaa acattttctg aataaccgtc attgtattga
    14461 taagcgacct ctacttcgat accttctttt tctcctgaaa aataaacaac tggtgtcaat
    14521 gtatcttttt cttcattcag ataatcaaca aattctttga ttccttcttc gtaatgaaaa
    14581 atttccttga ctggttcttc tccacgaaga tcagaaagag agattttaac gccttttaga
    14641 agaaaggcag attctcttaa ccgttcggct aaaatttcat atgaaaattt cgttgtactg
    14701 aatattgtat catctggtaa aaaatggaca gtcgtcccgt ttgctttttt tgtttttcct
    14761 atctttttca aagtaccgtc aggtttaccg ccgtttttaa atttctgctg atactctacg
    14821 ccatctctaa cgatcgttac aattagccat ttcgataagg catttaccac actggctcct
    14881 acaccgtgca gtccaccaga agttttgtac cctccttgac caaactttcc gccagcatgc
    14941 agcacagtaa aaatcacttc aactgttggt attccggaag catgcattcc tacaggcatc
    15001 ccacgtccgc tatctgcaac agttatacta ttgtcttcat ggatggtcac atcaatctca
    15061 ctgccataac ctgataatgc ttcgtcaact gcattatcga cgatttcata gactaaatga
    15121 tgcaagcctc ggctgtcagt ggatccaatg tacatccctg gtctttttct aactgcctct
    15181 aatccttcca atacttggat ggacgagtca ttgtattcgt tgtttacttt ttttgccaag
    15241 gcaaacgctc cttcaataat actttgcggc aaaaccgcac cacgattatc atactagaaa
    15301 aggtcgtgaa aaaaaagagg gtagacgaga aatcaatttt aattatctcc taaccgcttt
    15361 ttcgacattc ccctctatcg atcatctctt ctttgtggtc atttttattt ttggttttct
    15421 ttcaacttac caagttcaat cttaatacaa cgatccatta cgatatctgt acgtcctgct
    15481 tttcttagca tctcagctgc ttctttgttt tcaatcccta attgtgccca gaacacttta
    15541 gcatcagttt tcaaaaaatc acctgctatt tcaggtaaaa attcactccg tctaaataca
    15601 tctacgatat cgattggatc atcgatttct gtcaaattcg catagacttt ctcgcctaga
    15661 atttcttcac cagccaataa tggatttact ggtatgattt tatatccatt gtcttgcaat
    15721 acctgtgcaa ccatgtaact tgttcgttct ggtttattgc tcaagcctac gacggcaata
    15781 ttttttgctt cttccaaata gttgaaaatc gtttcttgtg ttggattttt tacagacatt
    15841 tctgccaccc cttgcttttc tttttattcc atctattata cttgtatctt tcattttttc
    15901 aattgttcct gttacctatt catgctaaaa taaaggatag ttttcaactt gaaagagggt
    15961 aaaaaatgaa attgatctta ttacttattt tagcatatct tttaggttct attccttccg
    16021 gtgtctggat tggcaaattg tttttcaaaa aagatatccg acaacacggg agcggtaata
    16081 ctggaacgac caatacattt cgtgtattag gcaaaacagc cgggattgtt gtcctgctta
    16141 tggatatttt aaaaggtaca ttggctactt gtttgccgat gatcttccac ctttctggga
    16201 ttaaccctct ttggtttggg gtatgcgcca ttcttggcca tacatttccg atttttgctg
    16261 ggttcaaagg aggaaaagct gttgcgacaa gtgcaggaat gcttttagga tttaatcctg
    16321 tattttttgt ttattcttgt atcatcttta tcgttagttt attttgtaca agtatggtga
    16381 gtttgagcag tatgatcagt gcagtattga tcactttatc tacgatcgtc cttccttatg
    16441 tagctcctgt gattttggct aagcccaatt ggttactgac gatcattgcc tttttagttt
    16501 cttcctttat cttttatcgg cataaagaca atatcaagcg tatcagaaac catactgaaa
    16561 gtcgaatccc gtttggtttg aatagcagca agaaaaaatc ctgatacttt ttcattccaa
    16621 agagataaaa cgggagtgtg acaaatcgat gtcacactcc cttattactt acaggatagc
    16681 ttttttacac accttggtaa acggtgttcc ctgctaactt ctcggtcaca acctcttact
    16741 tcactgtgat ggaatatttt gtcttgaaga tttcatgggc acctagttga ttgatcccta
    16801 ctttttccat caagtttcca ctactgttca ccgtgtcagc gattccgcac cacggttcga
    16861 tacaaacgaa aggcgcttct tttggataag gagaccagat ccctacataa ggcatatctt
    16921 tatagcttaa agttacgcta tgggaagatt tttcacttcg aatggcgtac gcatttagac
    16981 cccttgtttc aaaaaccaag gcatcttgct caaaaagttc acgattcaaa gccagattcg
    17041 tatttgtctg acctaacgtc ttttgatcca tatcgatgaa tgggcctttc aatggtaggc
    17101 gtaatcttga tttcaatgga gaaaatgcca gatagaaatc ttcaaatgac aatccttctt
    17161 caagaggtac attgaaagca ggatgtccac cgatagaaaa gaacatttct ttttcaccgg
    17221 tatttgccac ttgataatga accgtgatgc cgtctcctcc taattcatag ctgatcacta
    17281 attcaaaatc aaatggatac ttttctaatg tctccggcgt actccttaag atgaaactag
    17341 ccatttcttg tccatgttca atgacttcaa aatccatatc ccgagcaaat ccgtgttgcc
    17401 ccatttgata tgttttccct tgataaacat actgatcatc ttttaaacgt ccaacaaaag
    17461 ggaataaaac cggtgcatgt ctgccccaat acgctggatc accttgccag atatactcta
    17521 aaccatgttc ttttgatttc agactgatca attccgcacc gtgttcagcg atttcggcga
    17581 tcagctggtc attttgaata ataacagtca ttgccattcc tccaacttcg acttactttt
    17641 taaaatggga aggctggaac aaacatttct atctgcccca gtccccttac ttctaataac
    17701 tgtgttttgc taactcgtct ttaaaacggg aattcaatac ttttaaataa gttcccttca
    17761 tgcctaacga acgagattca atgattccag ctgattccaa ttttcgtaaa gcattgacga
    17821 tcacagaacg agtgatgccg atactatcgg caatattaga agcagtcaga cgaccttcat
    17881 ctccatccaa tgcttcaaag atagcttgta cagccttcaa ttcgctataa gataatgtgt
    17941 tgattgccat ttggacagcc gttgcactcc gtacatctgc ttcgatgctt cgagacttct
    18001 gataaaggat ctgcataccg acaaccgtag ctccatattc tgctaagact agatcgtcat
    18061 ctgcaaacga ttgttccata cgagataaga taatcgtacc caaacgttcg ccagctccaa
    18121 agatcggaac gacagtcgtc agaccaaatg gatatttctc tcttgactct accggaaatg
    18181 cagtaatatc actagtaatt gggatattag cttcagtttt catcaactgt gccactgtat
    18241 cagtataaga ttcaggaaat tgtttctcaa caaacattgt tttgattcgt tcattgttca
    18301 catcgtgacg ttcattataa cctagtaata ctccgtcact tcctatgata taggtgttgc
    18361 tgtcaagaat accacctagt gtgacagcca ttttatcgta aggcaaatct gcttgcatgt
    18421 caaaagtatt cttttgctgc aaaagtgtgt ttatcttgcg ggttttcgtt agcaagtctg
    18481 tcattgtcgt cctccgattt tttataaaat atagcgactt aaatcttcat ttgccgcaat
    18541 tgagccaagt ttgtcatcca catatgcttc tgtgatcgtt atttctccca tttgcatatc
    18601 tggtgcttca aataatagat cttctagtag tttttccaaa atggtatgca gtctgcgtgc
    18661 gccgatatta tctgtgtcac ggttgacatc gaaagcgatt tgagcgatcc gttcaattgc
    18721 ttcttttgta aacgtaactg acacattttc tgtaccgatc aacgcgacgt attgttttac
    18781 taaagcatta tttggttcgg tcagaatctt cacaaaatct tcagctgtca aatcatctaa
    18841 ttctacccgt atcgggaagc gtccttgaag ttctggaatt agatcacttg gtttagacaa
    18901 atggaaagct cctgaagcaa tgaataggac gtgatctgtt tggatgatcc cgtattttgt
    18961 gtttacttgt gaaccttcga caattggcaa tatgtctctt tggacgcctt ctctggatac
    19021 ttctccagat tgctgtgatt tagaagtgat cttatcgatt tcatcgataa aaataattcc
    19081 actttcttcc gctaatttga ttgcctcact atggatatca gcatccttga ctaattttgc
    19141 tgattcttcc ttgatcaata actctaaagc ttctttgact gttaccgtcc gttccacttt
    19201 tttcttcggt gtcaaagcac ctagagtttc atttaggtcg atccccattt gctccaatcc
    19261 attgttcatc attggtgcag gtttcttttg gtcttcaact tcaatggtta cttcacgact
    19321 atccagtaaa cccttttgta gttgttccaa gactgtacgg cgattgattc gaatatcttc
    19381 tgtcacttct tcttgcggtt cagcggtatt ttgctgacta gcattaaaaa gattcatcat
    19441 ttgttcaaat tgattagtgg attgtttctg ttcttttttg ataccaggta ccaagatttt
    19501 taccaaacgt ttttctgcat tttttttagc acttgtgtat acacggctgt actgctcttt
    19561 ttctacgatt tggatcgcat tttctactaa atcccgaacc attgattcta catctcttcc
    19621 gacataaccg acttcagtaa attttgttgc ttcgactttg acaaatggtg cattgacgat
    19681 ttttgccaaa cgtcgtgcga tttctgtttt accgacccct gtaggtccga tcatcagcat
    19741 attttttggt gtaatatctt gttgcatttt ttcatctaac tgcaaacggc gatagcggtt
    19801 acgcaaagca acggcaacag atttttttgc tgcgtgctgt cctacgatat attcatctaa
    19861 ttcacttacg atttgtcttg gtgtttgttt taattcatac atatctactc acctcttaca
    19921 gttcttctac gataatatta tgattagtaa agatacagat atctgcggca atatttaacg
    19981 cattttctgc aatttctttc gcactcatgt cgttttgagc atagtttttc attgctcggg
    20041 ctgcagccaa cgcaaaattc cctcctgatc cgatagctaa gataccatca tctggtgtga
    20101 taacttctcc tgtacctgaa acaagaagca tttctttatc attcattacg atcagcatcg
    20161 cttcaagttt ttgcatagat tgctgtgtcc gccattcttg agctaattcg acagctgcgc
    20221 gttgtagatt cccgttatat tcgtttagtt tgccttcaaa tttttcttct aaagtaaacg
    20281 catctgcaac acttccggcg aaacctacaa caacttctcc attgtagata cgtcgtactt
    20341 tctttgcagt gcctttcatg acaacagatt cacccattgt tacttggcca tctccagcca
    20401 tagcaaattt cccgtctttt tcgacggcac agatagtggt tgaatgaaat ggtgattcga
    20461 ccatgtttgt ttcctcctaa atttgttctt ttccttattt ttacgctctt ggatggaaag
    20521 aacgatagtt tttttgtaaa ctttcttttg ttacatgggc atagatctgg gtagtcgata
    20581 aattggcatg tcctagcaat tcttggactg tccgcaaatc tgccccatta tttaataaat
    20641 gagtagcaaa tgtatggcgc agcatgtgag gatggatatc actatccaaa ctgctttttt
    20701 tgatcatttg attcaagaca tattcaattc cagtaggcgt gattggtttt ccatgatgat
    20761 tcacaaaaac gatatcatgc tcttcgtgat attttgtcat caatacctgg cggccttccc
    20821 tgaaatattc ttgcagagca tcttgagcaa aagaacctag aggagcatat ctctctttgt
    20881 tccctttacc atggatcaac atgacctctg aatcaaaatc aatgtctgat accgacaaac
    20941 tgctacactc acttaaacgg atgcctgacc cgtaaaggac ttccagtaaa gcgcggttcc
    21001 tcaaatccaa aggactatct cctgctacgc tgtcaaaaag tgcttgcatt tcattttcat
    21061 aaaaaaaacg tggcagtctc aaatttttct ttttgagatg aacgtaagaa aatggatttt
    21121 cttttatcac ttcttctttg agaagatact gataaaacga acgtaggctg gctatcttgc
    21181 gactgataga atttctgcta tagctaggac gatgatcagc taatgcactc aaatagacac
    21241 gaaccgtaaa atattccgcc ttcagcagat cagcgtctcc agattcctcc aaaaaatgtt
    21301 tgaaatggag aatgtcttct tcatatgctt ctttcgtttt ttctgaatat cctctttcga
    21361 caatcaaata acgcaaaaat ttttgttccc acgtggcttc tgtcattttc tcaccttcct
    21421 tttgtcccag agaataattt agcacagcgt ttataaaaag acaaataaat tgtcagaatt
    21481 taaccaatat ttctaagttt gttcacaatt ggcgcaattc ctcacatttt gtttgagaaa
    21541 ttcttaagaa aaacgcaaga aaaagagcga gactatccgt ctcaccctct tcatggagtt
    21601 tccttactca tttgggattg cagttttttc taattctgtt tttattgcct cattagcttt
    21661 taaagctcgt tgtgcaattg cttcatatct tgcttttttg tctcgaatgc gctctggcag
    21721 ttcaggaaac agaccatagt tggcattcat cggttggaaa tgttttcctt ccgcatgagt
    21781 aatgtagtaa gccatacttc caatagctgt ttcttgtgga aactcgactg gttccatttc
    21841 ttttgctaag cgtgcggcgt tgattccagc aagtagtccg ctcgctgcac tttcaacata
    21901 gccttctaca ccagtcatct gacctgcaaa gaacagatct tcgcgtttcg ttgattggta
    21961 agtcggtttc agcaattctg gcgaattcat aaagctgttt cggtgcatca caccatagcg
    22021 aacgaattca gcattttcta acccaggaat catccggaaa acacgttttt gctcgcccca
    22081 tttcaagtga gtctgaaagc ccacgatgtt gtataaagaa gctgcagcat tgtcttgacg
    22141 cagctggata acagcataag gacgtttgcc agttttagga tcttccaatc caactggttt
    22201 cataggtccg aaaagcatcg ttttgaatcc tcgctgtgcc attacttcaa ttggcataca
    22261 tccttcaaaa tatttttctt tctcaaaaga tttaagttct gccacttcgg cattgattag
    22321 tgcctcatgg aaagctttga attcctcttc tgtcatcgga cagttcaagt aagctgcttc
    22381 tcctttgtca tagcgggact tcaaatatac tttatccata tcgatcgtag ctttgtctac
    22441 gattggtgca gctgcatcat agaaataaaa accatctgaa ccattgaatg ctttgatgct
    22501 ttctgctaat ggatgagaag tcaaaggtcc tgttgctact acggtgattc cttccggtat
    22561 ttcagtaatt tcttcgttta tcactgttac taatggatgc tctttgactt ttcgggtaat
    22621 tgtttcagaa aaagaatcgc ggtcaacagc taaggcacca ccagctggca ctgctgtttc
    22681 atctgccgaa gtgattacta ccgaattcaa tcggcgcatt tcttctttca ataaccctac
    22741 tgcattagcc aaactgtttc ctcttaatga attggaacat actagttcag caaaattact
    22801 tgtgtgatgc gcctcagtcg atttgactgg acgcatttca tataaacgga cagggactcc
    22861 tgcttgtgcg atctgccaag cagcttcact tccggcaagc cctgctccaa caacatttac
    22921 cattttcaat gaaagaacct cttttctatt atttttggat gtcttcttcg taatctccat
    22981 ttacacagac tacttgttta ccgcctttga ctttcttctc gaccagatat ccgtcacatt
    23041 ttggacaatt tctgcccact ggtttatccc aagaagtgaa ttcacattct gggaaacggg
    23101 aacaaccata gaatagtctg tttttcttag attttcgttc aatgacttgt ccttcatgac
    23161 atactgggca agtcacaccg atttctttta cgattggttt cgtattccga caatcaggaa
    23221 aattactgca agcataaaac ttcccatatt tccctaattt tatcaccatc ggatgtccac
    23281 agacatcaca atcgaatcct gctggttcat ctttgatttg aattttttcg attttttctt
    23341 ccgcattcgt cagttctttt tcaaacggtt tatagaaacg atcaaccact ttgacccacg
    23401 cttccgtgcc ttcttcaatt ttatccaaat ctccttccat ttctgctgtg aaatggatat
    23461 cgacgatttg agggaagaat tcacagatta atgaattgac aatttctcct agttcagtcg
    23521 gctcaaatct tttttgtgtc aattttacat agtagcgtct ttggatcgta tcaagtgttg
    23581 gtgcataagt ggaaggtcgc cctaccccgt tttcttctag tgcacgaatc aacgtagcct
    23641 cactaaaacg tgctggtggt tgagtaaaat gttgttttgg ttcaatatca acagattgga
    23701 ctacatcgcc ttcttcaagt tctggaagaa tattttcttt ctcttctttg ccgtcatctc
    23761 taccttccac atacacttgc atgaatcctt tgaatttgat ttttgaaccg ttcgcaatga
    23821 agatgacacc attttgttct aatgtcactt tcattgtatc taaaatagct ggtgtcatct
    23881 gactagccac aaaacgtgac cagatcaatg tataaagttt caattgatct ttatctaaat
    23941 attgtttgag ttcatctggt gtacgaagca cactcgttgg acgcacagct tcatgggcat
    24001 cttgtgcgcc ttgagcattt ttgatctttc ggtgatcatg tctagaaaat tcttttccat
    24061 acgtatcttc aataaatgct gcggcctctg cttttgctgt atcggcaatc cgggtagaat
    24121 cagtacgcat gtatgtaatc aagccgactg ttccttgttt acccaaagtg attccttcat
    24181 ataattgctg tgctaccatc atggtttttc ttgttcgaaa gtttaactta cgggcagctt
    24241 cttgttgcag gctacttgta gtgaaaggat tcgcagggtt tcttttgcgc tctttctttt
    24301 ctactttttt aacttcgaag tctgttccat caattctagc agtgacttct tttacacttt
    24361 gtgcatctgg taatttcttt ttcttaccgt cgattcccca gaaatttgct ttgaatttct
    24421 tgcgagcttt tttaaaatta ccgtcgatgc tccaatattc ttctggaaca aattctcgga
    24481 tctctttttc acggtcaata ataattttta aagcgacaga ttggacacgt cctgcactta
    24541 accctttctt gaccttacgc cagagaatag gactgatcga ataaccaacg attctgtcta
    24601 aggtacgacg agcttgctgt gcatctacta aatctacatc gatcgttctt ggttccttaa
    24661 aagctgcttt gactgcgtct tttgtgattt cattgaagac cacccgattt ttttctttca
    24721 gatccaaccc aagtaggtac gctaaatgcc aagcaatcgc ttctccttct ctatccgggt
    24781 cacttgccaa gtaaactttt tgagcttttt ttgctgcggc tttcaggctt ttgatgacat
    24841 cgccttttcc gcgtatagaa atataatggg gttcataatt gttttcaaaa tcgataccca
    24901 ttttactttt cggtaaatct cttatatggc caacactggc cattacttta tagtttcgtc
    24961 caagatattt ctcaattgtc ttggcttttg caggtgattc tacaatcact aaatatttat
    25021 acgccattac tgaatggact cctttccatt gtcgtgttcc tactattaaa agaggattca
    25081 tacaaatcgt agctaatcat atccattcag aaaacgtttg tcaagaaaag caaatgaaaa
    25141 gtatggtgtt attctttaaa ttctgccaag atatcttcac ccgaaaccgt gcaaactgca
    25201 ccgtcttgga tcaaatgatg acatccggta gaatgttctg ttaaaatatt tcctggaaca
    25261 gaaaaaactg ttcttccaag atccaatgct tgctgtgctg ttatcagaga gccgcttttt
    25321 ttcttggctt caaagacaca tgttccttta gacaaagctg cgataatccg atttctcatt
    25381 ggaaaacaat gctttttcgg gccggattga ggaggatatt cactcaaaag aagatgatca
    25441 tttgcaatca cctcttgcaa gtcacgattt tcagcaggat agacttgctc tatccctgtt
    25501 ccaatgactg cgattgtccg tccaccatga gagattgctg cttggtgacc gtatgtatct
    25561 atccctttcg cgcagccact gacgatcaca tatccagctt ctatcactgg ttttagtaaa
    25621 taataaactg tttttttggc taaagcagaa gtgtgtcttc caccaacaac gcttaacatg
    25681 ttacaagaaa gtaaagatag atcaccatta taaaacagta caattggcgg ataggtcaag
    25741 tgaagcagct gagggggata tttgggatca aagcaagtga taaacgactg tttcgattgg
    25801 attttgcccc agttagcgtg aatttcctgc caacttttgc gaaacagctc ccggtgtttg
    25861 gttattttac ctatcttaat gatgtcttca aatccgattt ctttataagg atactgcatc
    25921 atccagttag caagtgccca ttttccttga ctactgattc cttttgtata agcaagtttc
    25981 aataaatttt cttctatttg atacatcata aaaccgcctt tcgtctctct ttctaataag
    26041 atacgtaaaa gcggctgttt tttattgtaa atattttttt acaggtgcaa aacttttacg
    26101 atggattggc gtaattcctt gtcgttcaag tccttctaag tgttcctttg ttccataacc
    26161 tgcattatgc gagaaaccat atccaggata aagcacgtca tattctttca tcaattggtc
    26221 tcgatatact ttagcaatga tacttgcagc agcaatcgac acagatcgtg catccccttt
    26281 gattattttt tcctgctgaa taggcaaatc caaggtcatc gcatcgatca acaaatagct
    26341 tggtacaatg gacaattttt ctactgcttg gatcatcgct aatttacttg cttcataaat
    26401 attcacatca tcgattgtct gctgatcaac gatcccgatc ccgattgcca gggctttttc
    26461 ttgaacttgt tgataaagaa gttctcgctt tttctctgat aattgtttgg agtcattcag
    26521 ccctaatatg atctcatttt tcggcaagat aactgcagct gccacgacag gtccagctaa
    26581 tggtcctctt cctacttcat caatgccggc aatccattgg tgaccttgtg cgtacccttg
    26641 cttttcaaag caaaacattt cttcatattt ttccttaatt aatgcttcct ttttcaatcg
    26701 tcgttcaaaa gaagccagtg cttgctgcac gcctttgcgt ggatcatttc ttaatgattc
    26761 tagtgtagga tcatctattc tctgtatgct tttcaatcgt tctttgatac tttggatgga
    26821 ttcagtcatc acttttttgc tccatttctt cgcaacgatc cagtgtgtat cgtccaagtt
    26881 ttccttgacg aatatcatgc acgactaatt cgctgccacg atcatagtct tctttatacc
    26941 cacgttttgc tgtaatatcc atcagtaatt ccggtgccaa tttttcttct tgctcgattg
    27001 ataaaccata acgatcgctg attcttcctg aataatatcg ggcaaaaaag tctaatccgt
    27061 aaatcgctaa atcatctaaa tggacaagct ggtctttgat cgcgcctgtc agcgctagtt
    27121 ttttgccgat ttcttcatct tcaaatttgg gccataaaat ccctggtgtg tctaacaatt
    27181 ccaactcatt acctaaacga agccactgct gaccttttgt tacgccaggc ttatttcctg
    27241 tctgtgcaat tttcttgcct actagtcgat tcatcaacgt agatttccca acatttggaa
    27301 tacctaagac cattgcccga atagcgcgag gcttgatccc ccgagcagca tctcttgccc
    27361 gtttttcttt caatgcttct ttagccgcag gtactagacc tttaattcct ttactttctt
    27421 gtgccgtgat agctaatgca agatggcctt gtttacgaaa atattcttgc cacagctttg
    27481 tctgttgtgg atctgccaga tccgctttat tcaataaaac aaggcgaggt ttttgctgaa
    27541 tgatctgatc tagcattgga ttacgagaag ataaaggtaa tcgtgcgtct accaattcaa
    27601 atacgatgtc cacatatttc attttttcag aaacttctcg tcgggctttt gccatatgtc
    27661 ccggaaacca ttggatggtc attgttctct ccattccttt cataccggaa tattccccgg
    27721 ctttacttgt ttttttcttt ttcaatataa attcactttt ttattataaa gaaattccat
    27781 ctattagtta agtgaaagtg aaccagtcct cattctatca catcttcact tttttgatga
    27841 tgcaaatttt tattttggtt tctatatatg aatctaaatt tttgacagcg tttcctaaat
    27901 aagagatgat agacgaacaa aacagaaagg agacgaaaca aatgaatagt atggtttttg
    27961 ttaattttcc tgttgctgat gtcaatcgtt ccgttgcttt ttatgaaaag ctgggtttta
    28021 aaaaaaatga agaattttct actgaagaag ctggttcaat ggtatgggat gacacttttt
    28081 ggataatgct tctctctcat gatttttata aaaaatttat aaaagataaa gaaatagctg
    28141 ataataaaaa aacaagtggt gctttgattt cgtttagtgt tgaaaatgcg gatgaagtaa
    28201 aaaaatttgc tcaaattgca aaagaaaatg gtggtacttt ccacgaagta gatatgggaa
    28261 tgcctgaaga agaaatgttt tcattagaag tccaagatct agacggaaat acattagaac
    28321 ccgtatggat gaagatgtaa gcagataact aatctgacgt tttttataac aaaaaaacaa
    28381 aggcggatga tacgataagt attctccgcc tctatttttt ttattcgtac aatctctttt
    28441 ttacagttta taaaagcagt cttctcaccc tttttttaat tgttagatgc cagtgtatgt
    28501 aaatcaccaa tgtgtcatga cacattggtg attttatggt atccttttat tattaactga
    28561 caaaggagag aatattttga acactttgat tgcaaaagac gtttttagat tgcccgaaat
    28621 gaatgcaaga ttgttgtcga cctctgctct tttattagcc cttgatgtca tcttatacaa
    28681 gatagcgatt ggtcctgctt attttcaaat cacattcgga tttttaagta tggcgcttat
    28741 gggttatcta tatggaccga tctgggctgg tttgcttgaa atgttgtcga acactttaaa
    28801 tttcacgata tttggttcag gaacgtttca agctgctttt cttatcacgg ctttccttgg
    28861 tggatttatt aatggcttat ttttatatca taaaaagata aacctgcctt ccgtcatcgc
    28921 tgcccaatta ttgattatga tcccagtcag cctttttatc aattcttggc tgatgtctgt
    28981 tttgtttggt gcagatttca aagtaatttt tctaggtaga ttcatcagga atatgatcca
    29041 aatccctatt caaataatgg ttgtttattc atttatcaaa actttagagg ctagaaaagt
    29101 gtttaaacga gctgtttgat tttacgtact aaggtagtaa actgattttg gattaaacaa
    29161 taaagcgtgc agtctcaaca gactgcacgc tttattgttt ctcactggat ccattttatt
    29221 tcatcaagtg gataatagac aaatcttgct ttacctagaa tatcagtttt attgatcgtg
    29281 ccaaacgaac gactatcttt tgaaactctt cgattatctc caagtacaaa ataattatct
    29341 tccggaagct tggattgtcc cgttagctct tgtagtgtaa agtccgtcgt ataggatgca
    29401 tttgcatgat ctgatttcag gtttttagtt agataaggtt ccttaactac tttcccattg
    29461 atcttcaatt gatcattttg gtagctgaca ttttctcctg gaagaccgat tactcgtttg
    29521 atataaatcg tgccatctgc taattgaaaa acaacaatat caaatcgtct gatctccgaa
    29581 aatttttcca tcacaaccat atcaccttgc ttcaacacgt tttccattga attgccttcc
    29641 actggtaccg ggatgaataa aaatccccgc aacatgaacg caaggaatac agaaacaagt
    29701 aagtatttga atacaagcca gaagcgatta atatagcgtt gtttttttgt caatctctat
    29761 caatccatcc ctcttttttt cattctttct ttattataag aaaaatatca aaaaaatgcg
    29821 actagatagc tgaaataaaa ataattcttt caattaggct attggacaaa aaactttttt
    29881 gatcataaag tacaaaacta tctcttatat gtcatgatta ttttctaaat atttttccca
    29941 tgcttcgtca aagatatcca tggacggcaa atagtcgaca ttcagttcca gataattaga
    30001 aatttcttcg tagctagagc tgtgcttagg aaattggatg tctttaccgg cttctgtagc
    30061 gaaaacagat aaagcatcat gtgtgccacc gcctcgcagt gtcattaaat aatgaaaaaa
    30121 gcttcgattc acccttcgtt cctccaattt tcttcgataa aggcagcccg gcgtgcatgg
    30181 tttcttttaa aattttcagg atgcttttta taatactctt gatggtcttc ttctgctaga
    30241 taaaatggtt gggctggttc gatttttgta acgattggct gttcgaaacg tccactttca
    30301 gctaactgtt tcttactttt ttcagcaatg atccgctgtt cttctgtcaa ataaaagatc
    30361 accgggcgat aattgtcacc gcgatcctcg aattgtccga aagcgtcagt tggatcagtt
    30421 tgctgccagt aaagttctac taattgttca tatgaaatca atgttggatc atagataatc
    30481 tccactgctt ctgtatgtcc agtcgtttgg ctttttactt gttcataagt cggatctggt
    30541 acatgccctc cagtatatcc tgacatcact gtatggatac caggaagcgt gtcgaaaggc
    30601 tggatcatac accagaagca gcctcctgca aatattgctc gttccattat gattccccct
    30661 ctaatactac tcatccgatg attttttatt ttcttttgtc ttgctttcag tactgctggt
    30721 gctagttttg ttggtatttg agttgtttga agcatcagag gtatcttttg gcaaatatag
    30781 actaaaccga atgtcatcat ttactaagtc gattttatct gctttaatat acatcccgtt
    30841 ttgcattcgg aattgatcca acctaagcat gatagtcaga tcatctgtat tgacttcgat
    30901 ccaatctggt aatttatagt ttctcttgat catattcatc acatctttga ttggcaaact
    30961 caatgttccg atagaaagac ttctagcttt tagttggaca tttccgtttt ccatcacata
    31021 cggatcaaaa tataaataga aatcgatagg aaaacctagt acttcgaaag taccattgag
    31081 tagtgcttca ttttccaaat aaaatttata ttcaatatca ctatcttttt gatactcact
    31141 aaggaaaaag ctgatgattt gattgacttt ttctttattt gaattgatcg ttaagacagg
    31201 atctccttgt ctttccgtaa gagctggtat ttctgggagt tctggttccc gattagcaaa
    31261 aatacggctg cctaaaaata cggtcgaacc aagtactacc ccaagaagga taaggaaggc
    31321 gaatttccag taatttgttt tctttgtttt gtttgaagat acgtcagatc ttgttttcat
    31381 gtttattgat catcctcctt ctctaaccat tggttttgtg tctcgatcag ctgatctctc
    31441 actgcatttg ccatcaattg ataacctaaa ttatttggat ggaaatgatc ttcttcatag
    31501 agcacattat ttttgatgcc atttagattt tctgtcgtgc tagttgtttg atctgttcct
    31561 tcttctgtga cacctacttc ctgattcaat ccttgataaa gcaaatcatt gattgggata
    31621 aagtagcagt tcggcatttt ttttgtcacc gtttctgttc ctttgttcca attgttaaca
    31681 attgtctgca tgtcagtgat ctcaggaaag ttcaaataaa atggattgta gatgcctaaa
    31741 atgtaaatag gtgcggtttt attgagggat cgaaattctt ccaatagttc tgtgacattt
    31801 tcctgatagt ttttcagtgg ctttttaaag gaattgaccg ttaacccgaa taaattattt
    31861 tggataactt tcatcaaatc attaccgcca acagtcagtg tgataaaatc agctgttttg
    31921 atattgtttc gaatttcttc ttttgttttt actcgcttta agatctggtc acttcgttca
    31981 ccatttacac catagttttc tgtttcaacc gcggtcaaat tataacggtc ttttagatca
    32041 tttgcgacaa ggggaacgaa tcctccttgc ttggtttgat ctcctactcc ttcagtcagc
    32101 gaatctccga tagctatgta gtgaagtttt tcttttttat ttggatcttc tctcacagta
    32161 gttactggtt tcattagtgg gccagctttc ggtatagaag cagctaaacc tgcatagatg
    32221 atgatcgcaa ttaataatgg gaacaaaacg aggatcaaat gtcgtttcgt taatttcatt
    32281 ttttcacctc ttgtattcga ttggttctaa tgttatcaaa aattttatca aaaaggtagt
    32341 ctttatgaaa aaagaaaaca attattttcc taattttata aagaaaaaga gaataagctg
    32401 caagcctttc aggctgaggg cttatcctct tttcgactaa tctacataat acatgattgc
    32461 aaacgcgtct ttaccagtat gcgtagcgat aaccggattc gtatgcagta cagggatatg
    32521 catgtccggg aataattctt gcaaaccctc tttgaattct tgcgccaatt cttttgcatc
    32581 cgcatgtgaa ataccgattt gtcgcacatt tggcaattct ttcagttcat ttttcaaatt
    32641 attaaaccat ttatcgaacg ttttctttcc tcgcccctta gttacaggga ttaattcaga
    32701 atgggcgaaa ttcatcacca cgcgtatgtt caaaacacta gacagcaaac cagttgttcg
    32761 gctgattctt ccacctttta ccaagttatc tagtgtggat attccgatgt ataattttgt
    32821 attgtcttgt acttttttga cttcttcaag gattttctct actgttttac cttcttttgc
    32881 taattcagct gctcgaatta cctgaaaaga taatccttgg tcagtggtat cactgtcgat
    32941 aaccgttact ttcgtttttg ttagattact ggcttgtctt gctgcttcta ctgttccact
    33001 caagcctttt gtcatatgga tcgaaatcac ttcactgccg tcttctccta gctcatcata
    33061 catcgagatg aattctccaa ttggtggttg gcttgttttg ggcagatttt ttgctttcgc
    33121 catcatttcc ataaatcttt cgccgtctag ttggtcatca tcgggatata ctgttccgtc
    33181 gatcatgatg gatagcggca tgacacgaat gcctaaagca tcgcgtacat ttttttccat
    33241 cgtacatgaa gaatctgtta cgatttttac atttgtcatt attttctctc tctctttctt
    33301 taacaagatg ccttattcat gttaaactac atgtaagaca atttctattt ttagtataac
    33361 agacaaataa tgatttttca ttaattacac ctagtaaagg agccgttttc atggaaaaaa
    33421 cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc acccatggaa
    33481 ttgggactgg attgagtatc gcgggactag tggttctttt agtaaaaggt gcccacttgc
    33541 attcctccct ccatattgtc tcttatgcta tctatggatc gatgatgatt cttttatttt
    33601 taacgtctac tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg ttccaagtat
    33661 tcgatcatag ttcgattttt cttttgattg caggaagtta tactcctttt tgtttgctga
    33721 gtatccgagg atggctaggt tggactttat ttgtattaat ctggttgttt gcgattcttg
    33781 gcattatata taaagcgttg acgttgcata aacgggagag agtatcaaaa gtatctacga
    33841 tcatctatat ttttatgggg tggctctgtg tcgtggctgc tgttcctctt tatcactctc
    33901 ttggaatgac tggtgttatt ttgatggtag caggcgggct ttcttacacg ataggcgcat
    33961 tcttttatag cttgaattct atccgctata tgcatgttgt ttggcatttg tttgttatgt
    34021 taggtgcagg ttttatgtac ttctctgttt tattctttac ttaataaaaa aaataaagct
    34081 atggatggga cagctctaaa taacagctag tctatccata gcttttttat atcgtcaatt
    34141 cgcctgtccc gctttttaca ggattctcat tacgctgata cgtttcaaat tgatgcggat
    34201 aacgattttt ttcatccatt tctccttgac tagtattgat cagtgaccaa tcagaccagt
    34261 caacaggtgg aaaataagca tctccatcga atacatgatg gatgactgta cggtataata
    34321 tcgtacaaaa tggcagaaat tcttgataaa cagctgaccc tccagcgatg aaaaagatac
    34381 cgtctgtttc tttttccaga gcaagcacgt cttccacact atgcatgaca tgcacgcctt
    34441 ctgctttata ggaagaatct ctcgtcaaaa caatcgtctg acgattcggc aacggacggc
    34501 tacccatccc ttcgaatgtt tttcgtccca tcactagtgt atttgcttct gtcatttgtt
    34561 tgaagaattt cagatcattt ggcaaacgcc aaggaagacg gtcttctttt ccaatcaatc
    34621 cgttttcatc ctgtgcccaa attgcagcta acattttctc atcctttctg attctaaaca
    34681 gcgataggag ctttgatcgc cggatgtggg tcatatcctt ctaattggat atcttccatc
    34741 tcaaaatcaa atactgactg cttttcctga ttcaaaacta aagtcggaaa tgcgcgtatt
    34801 tctcggctca attgttcttt catttgttcg atatgattga tataaagatg agcatctcct
    34861 aatgtgtgaa caaattcgcc aaccgctaaa cctgtttcat gtgcaattag gtgagtcagc
    34921 aacgcataac ttgcaatgtt gaacggaacg cctagaaaca catccccact gcgttgatac
    34981 aattggcagc ttagccgtcc ctcttgaaca taaaattgaa acatcgtatg acaaggaggc
    35041 aaagccatgc taggaacatc ttccggattc catgcagaaa cgatcaatct tctagaatca
    35101 ggagtctttt tgattgcttc gatcacttgt ttgatttgat cgataaagcc accgtctttt
    35161 gtttcccagt gacgccattg atagccgtag atgtgtccaa gatcgccgta tgtttcagca
    35221 aaggaatcat cggttaggat cttctcgcaa aaaagcttgc tctgcttttt atattcttca
    35281 ttgaattcag gatctgtcaa acatctatgg ccaaaatcag tcatatctgg tccattatag
    35341 tctggacttt tgatgtagcg ttcaaatgcc cattcatccc aaatatggtt tttattttgt
    35401 aatagatagc ggatattcgt atctcctttt aaaaaccaaa gcaattcgct tttgatcaat
    35461 ccaaaaggta ctcgtttcgt tgttaacaaa ggaaaccctt tttgcagatc aaaccgcatt
    35521 tggtatccga ataagcttaa tgtgcctgtg cctgttctgt cttctttacg atgcccttct
    35581 tccaacaatt ttcttcctaa gtcaagatac gcttgttcca tcctttttcc tcctaatctt
    35641 ctacccattc actcaagtat tcccaccgtg ccattttttc ttcaagttgt gcttctgttt
    35701 cagatacgtc gttttgcaat tcttgtaatc gagtgaaatt atctccttga tgattcatct
    35761 cttcttggag caatgagatt ttttcttcaa gttctgcaat ctcatcttca atcgtttccc
    35821 attctttttg ttccatgtaa gataatttct tcttttcagc aggttccttg ctctgtattt
    35881 ttggtttcgc tttttcagca ggctgctggg tctctttttg cttcagtaaa taactggaca
    35941 tgtttccgta ataaagttct tgttttccgt ttccttggaa tactaataac ttatctgcta
    36001 ccttgtccaa gaaataacgg tcatgggaaa cagtgatcac agcacctgga aaacttctaa
    36061 aataatcttc caaaatcgtc aatgttgcta tgtccaaatc attggtcggt tcatccaata
    36121 ataaaacatt aggttgctga atcagcagtt tcaacaaaaa taaccggcgt ttttctccgc
    36181 ctgaaagttt gcctatcagt gtcccatgca taaatcttgg aaataaaaat cgttcaagta
    36241 attctgctac tccgatttga ctgccatctg ctgtttttgc ttcttcagca gcttcttgta
    36301 agtaagcaat catccgttta tctggtgcca tttcttcatt ttcttgtgta taatacgcaa
    36361 gatgaaccgt ttcaccgata gacattgtac cgctatccag gggaattctg ccagctagaa
    36421 tattaagcaa tgtcgatttt cccgcaccat ttttaccagt aatacctagt ctttcccgag
    36481 attgaatcaa taaatctaaa tgttctagca gtgtttggtc atttatacgg taactgccat
    36541 ctttgatttc cagtactttt ttccctaaac gttgtgtcgc taggttcagc tctaaatcat
    36601 cttcttggtt tacttggtac aagttctcct ttagatcttc gaaacggttg atccgtgctt
    36661 gctgcttggt gccacgtgcc tgtacgccag ccctcatcca agctaattcc tgtttataaa
    36721 gccgcttgcg cttttcttct tgttctactt ctacacgatc acgttctgct ttttctaaga
    36781 cgtaagcttc ataatttcct ttatactcgt ataattttcc aaatgaaagt tcgaagattc
    36841 ggtttgctac tctatctaga aaataacgat cgtgggtaac catcaatagt gcgccacggt
    36901 attgtttcaa atagttttcc aaccactcga tcgcttcata atctaaatgg tttgttggtt
    36961 cgtctagtaa tagtaaatcg ggttcatcaa tcaacacttg cgccagacta actcgttttt
    37021 tctgaccacc tgataaagta ctgatttttt tatctaattc actgattcct aatttttgca
    37081 aaataatctt tgcgttggta tctgtagtcc aagcatcttc tttattcatt tgttcttctg
    37141 ctttagcata acgtttttgc acatcagggt cttctccgtt ttcccctagt tcgatcaatg
    37201 cttcctcata tgctcgaaca gtttggatca aggggctgtt tccttgaaag actgcttgga
    37261 gaacagttaa ttcatcggaa aattcagctg cttgagaaag ataaccaata cggtaatcag
    37321 ttggataaaa cactgtttgc cgatcaccgt cgctgctatc gataccggca ataatattta
    37381 agagacttgt tttacccgtc ccgttcgtcc caatcaaccc gatacggtct ttttcgtgga
    37441 ccaaaaaaga aatctggtca aacaaatctt tttcaccata agtttttttg aggtcagtta
    37501 cttttaattc tttcaacatg ttccctcctt atcttggata ttgtatcata aaaatagtga
    37561 acttcaggaa aatttttttg gtatttgttc tttttgtgtt ctcaaaaaaa gaacaggacc
    37621 gattcgatcc tgttctgata tgttttgttt tttataaaac ttgaaaattt ttaccttttt
    37681 attttattgg actcgttttt tttgactttg ctcgaaacgc aaatcggaaa gaaaagttat
    37741 cagatggttt gttcatcgta taagattcta acaaattgat attcctgcga tagctgttcg
    37801 cagaaaacgt cgtgattttt tcagcaactt cgtattcatc aatgattttt ctttcaagat
    37861 aataacctct tagcattttt ttcgtaaaca gactatcttg ctggatcatc cacgaagaaa
    37921 tcaaattccc cgagttcatt tttattcctt caagtttgcg ttgctggata aaaaattgaa
    37981 ccatcgtttc atcataaata tcagtcaagt tatctaaact ttcaataatc aactgagtat
    38041 ttttttgata tgtctctctg atttcttgga tatcttcatc actaataaat gtctgacgat
    38101 cagcatatct tctttgccaa aacattttcg gatggagaac gattcgcatt aacctacgga
    38161 caacaatgaa ccaaaagccg ataaaagaca aaatctggct tgtgatagaa tgttcgaatc
    38221 gagaaagaaa acgggagtaa aaacgataac cattagaaga aagcttgccc gaacgataac
    38281 tttcatccaa tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct tgtacttctt
    38341 gcttttcatc atcggttaaa tcttccaagt acagatcacg tatccgttct tgataatttt
    38401 ctatcactgc ttcagtggct aaaaattctt ttgtattacg atcaatttcc tttatttctt
    38461 ttcgcaaaac ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc gtgcttttcg
    38521 cttcctctgt ttccgtcagc ataggcaaga caatgatccc tatgaccaaa gtcactaaga
    38581 tgacacccgc cgtcagaaac aaaagcaaag accgctggta aaacatcata ttattgattg
    38641 aaaatggcaa aataaaaatc gtagccaaac tgactgttcc ctttacaccg ccaaatgtta
    38701 agatcaagat ctcgttcaac tgttttttga actttttact gccatctttg aatacataaa
    38761 aaagactgat agaaatgaat ctgatcacaa aaagcatgac agagattaat acgataatcg
    38821 ctaataaaag accgtttgga tataaaccgt ctccccatac tggcgagaac acttgcgtca
    38881 attcgattcc taaaaatata aatactaatg cattcagtgt aaaagcaata gttgtccatg
    38941 tactgtggga caagctcgac aattctgcat caaacacaga gattttccta aaaccagaag
    39001 cttggagaat acctgctgcc actgcagcaa tgattccaga tacatcaaat gcctctgcca
    39061 atacataggc tagaaaagga agcaaaagtt cgatcagcag ataccctgtg acatcttgtg
    39121 ctgatgcttt ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc aaaaagccga
    39181 tcaaagctcc cccaatacta gaaactacta gactcatccc cgcgtttact gctgaaaaag
    39241 atccggtgat cagcgcacca agagcaaact ggaaggcagt aacaccagat gcatcattca
    39301 aaagaccttc tccttccaaa atatgcatcg cctttttagg gatattcact cgtccagaaa
    39361 gagaactgac tgctaccgca tctgtcggac ctaacgctgc tccaaaagca aagcaggcag
    39421 caagtgggac acttggcaac aggaatgata acgtcgcacc taccccgaca agggtcaaaa
    39481 tgactcctcc aaatgccaag aacaaaattg tcccgaaatt tttgagaatc gaagaaatat
    39541 ctgctttttc tccttctcta aataacaatg gtgcaataat cataactaag aacaattcag
    39601 gttcaaaatc gattgattgt ccccactctg tcagacctaa taccactcca aggaaaattt
    39661 ggatcagtgg agctggaatc attggcaaaa tgcggctagc tatgttcgat aaggtgatcg
    39721 ttactgcaaa tacaataact aactcagcaa attccatcgt ccatcccctt tattttttat
    39781 tttgtgtaag catttttaaa aattgttctt ttttctttaa aattttggtt tcgactactt
    39841 caacctgtgt ttggttcccc tctaattcac actgtacacg ttttgactcc aaacgaagca
    39901 gatcatgttt cagacgttcc aactgacgat acatcaatat atttctttta cgcaacttac
    39961 aaagtatttg acggcggtca tccatcgaaa gggcgccata gcgatcatgc aacagttttt
    40021 tttgttgttc aaaactctct ctcattttcc agatctccta tacacaaact tccatcatta
    40081 tagcaaagtt ctttttggat gaaaaatatt tcattgtttc atcgtttatt gctagttaga
    40141 aatttccata aatttttcaa cgcctcattc tattccaaat agtatcagat atcaagtaaa
    40201 acttaagaaa gataaaagtt tctgacaaaa ggatgtttac tttcccattc ctgcctttct
    40261 tgttctttta tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac agtcccttat
    40321 tctgaataaa cggtgtggaa gagacagcct atcagtagta ataagacaca tctctttcac
    40381 agcctcattt ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac atcttctaca
    40441 cgttgatctg gattgatcac gatatacaag ttacctgttt tttcgctgat ttttgttgat
    40501 tgatattgat tgtctaatcc ttctgttcct ttatctagat atgggaagta cacaagatca
    40561 ggtgtgccat ttcggtaaag gatttccatt cgctcgatat cttcgtattt caatgcgcga
    40621 ttaaacatgc ccatttccag tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct
    40681 tcatggaaga tttcgatttt aaatccagca cacggatgga tttcaacaaa ttcactgcca
    40741 ttgattcgtc caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg
    40801 ttgatcgtcc aggtttctcc atttctaaaa taaaatttta gcgctgtcat ttctttactc
    40861 attaaaaaga ctcctttatg atgtaattta ttcttgtact acctcagttt agcacaaatt
    40921 tatgtaagtg gtcacatctt atgttttttc ttctaatgac gtatctgccc actttaagta
    40981 gccaacaaag ctgagcagat acagtctagt gttttttaaa tcaatttttc tttcgataaa
    41041 aaatccaaaa cctgtttttt atcatttttt attctttgtt ctacgacggc tttttcagct
    41101 aattgcaaag cttcactaac ccacttacca ggtttttttc cagtttcttc tagcaagatt
    41161 ttaccatcta ctgctaattc gcttaatgac ttgataggta aacgatcata ggattctcct
    41221 acggcatcca tctgaggtgt ttcgtcaaaa tagatcatca attcttctac ttgcagtgca
    41281 tgttttttgc ctaagaagta caattcgaat ggttccaaag ctccagtttc tcgttgtcgt
    41341 agtccttgga caagtgcttg aacgtcttta atcatttggt tagaacactt ccacgatttc
    41401 aaaaaggaac gaatctcatt ttcttttaga tccagttggc tgatcaataa tgcccatgct
    41461 tggctctctt ctttcaatgg gacggatggt aattcagcaa aacgtaaaag tgcttcgcct
    41521 tcttctttca aaccgggaca gcaaatgtag cactctgttt caataaatgc atggattccc
    41581 gcggcccgtt catttcccaa gagcatcttg atgaattcca catttatacg ttctacagat
    41641 attttaccta atagctcgtg gttttctttg atggcgtaga aggtttcttc ctctaatttg
    41701 aaccctagtt gactgacaaa acgcaacccg cgcatcattc gtaaggcatc ttcatggaac
    41761 cgctcatgag gattgcctac agctcgcaag atctgttctt ctagatcttg cagaccatcg
    41821 aacaaatcga tgatttctcc atcttctttc aaagcaaacg cattgatcgt aaaatctctt
    41881 cttttcaaat cttctgccaa cgaacgaacg aattcgacat gatctggtct acggtagtcc
    41941 tgataagttg attccgtccg aaatgtggtg atctcatatt gttcatcatt ttctaatacc
    42001 aacacagttc catgttcgat cccaatatct atcgtttttg gaaaaagctg tttgatttct
    42061 tctggaaatg cgcttgttgc gatatccaca tcatggattg gctggttcag caagatatca
    42121 cgaacacttc cacctacaaa ataagcttca tatcctgcat tctctaattt tttcagaact
    42181 ggaattgcct tttggtattc cagaggtaat tgttttaatt tcatttttcc aacctttcct
    42241 gcgtgtttct tgtcctgaga taaagactca gttgcatggt gactgtttga caagtccgtt
    42301 tcagagtctt caaggccagg accacttcat cttgtttact cgtccttacg ctcaaagcga
    42361 ttatggtctc tttgattgaa tttttccatg tttttttcaa aaacttcagt taagtcgata
    42421 ttcaatgaat tagccatgat catcgtaacg aataaaacat caccaagttc ttctgctacc
    42481 gagttaactg gttcagaagc ctttttgctt ttttctccat aagtgtggtt gacttctctt
    42541 gccaattcac ctacttcttc agttaatcga gccatctgag ctaatggaga gaaatacccc
    42601 accttgaatt gctggatata ttcatccact tcctgctgca tactgtataa agaacgcttt
    42661 ttatccatct ttttccctcc aaaactatct taccaaaact ttaacgcaaa tcctagataa
    42721 gagcaattga gaaaatcgaa tttccatgct acatttagga aggaatttct aaatggaaat
    42781 tctgtgaaaa tacataattg aaagctggaa ttcatatggt caaacgaaaa acagtaaaag
    42841 aagttctttt tatcattata ggaacaagta tttatgcatt tggtcttgtt tacttgaaca
    42901 tcgctaatca tttagcagaa gggggagtct cagggatcac cttgatctta agagctttat
    42961 ttggtattga tccagcttat acgactttat taatcaacat cccattgatt ttactaggag
    43021 gaaagatcct cggtaaacgt tccttggcct atactgttct aggtacactt tctttgtcat
    43081 tctttttatg gatctggcaa agggttccac tagaaatcaa tttacagcat gatctactga
    43141 tcgtttctct acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg
    43201 gtggaacaac aggcggcagt gatattatcg ctcggatttt agaacaaaat tacggcatca
    43261 ctatggggcg ttccctattg gcatttgata tcatcgtctt acttgcttca ttaacctata
    43321 tcgacttgaa acgtatgatg tatacgttga ttgtttcgta tgttttcagt cgtgtgattg
    43381 attcgatctt agatggcgga tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg
    43441 aagagattgc accactactg atgaccggtc tgcaaagagg tgtgactttc ctttctggag
    43501 aaggcgcatt ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat
    43561 taactgaagc taagaggatc gtccacgaga ttgacgaaaa agctttcctt tcaatcatca
    43621 atgtgcatga agtggaaggg caaggcttta cttatttgaa gccacagaca agattactga
    43681 aaaaactata agaaaaaaga caaagatagg ctatgaggaa agattccaca tccagctgac
    43741 ctttgtcttt tttagtacta tactattttt aactaattgt tatgttcact tacccggtta
    43801 cttttctttt gtgtctgcaa aaggatcttt cggttaactt catcagataa tttcaaaaaa
    43861 gcatccctat ctccatcttc tagtgcttga tcgatttgag cataaagcat ttttagtttt
    43921 gcctcttctt ctttttcttg aaaatatcgt tcaattaatt cagtatcttc ttcactgact
    43981 gaagaatccc aagaagaaaa tggattgtct tctaagatag atagatattc tcttgtctgc
    44041 caagcatttt cgaatataca ttccacatat aaaggctttt tccaattcaa acggatttca
    44101 tgaaaaatct ggtctgtgtc cgtaaattct tttccctgca aaaacaattt catgggttcc
    44161 ccttcaacag atagatcact gatctgcagt cctcgatccg caaaattcgc cccttcaaca
    44221 aagtggacat tgcttaaaat cgcttcatga ttggcaagat aattcaagat ccaaaaaact
    44281 tctcttcggc taaaagatac atgattgacc atccacgtta aaaaattctt cttttcccgt
    44341 acatcaatta gcatttttct cacctctctg ttaggtcatc taataatgac agtgcctcca
    44401 tatctcctgg ttcaagtgtt aaataataag tcaataattc ttgcgctctt ttcagctgcc
    44461 cttcttctct taagaaaaag gcatactctt tcaagaaatc tggttcatgt gatagttcat
    44521 gataagcctg ttcatagtgt acagctgcaa cggtaaagtc ttctaattca ttataagcat
    44581 gggctaggtt ccattctgca taaggattag cagtttcttc catctgattg atcacttgta
    44641 tcacatcttc atatctttca tcatccaagt aaagattact taaagtaagt aatgtttcat
    44701 cttgtttgtc tcctacttct aaagctttca gtaaaagttc ttctgctcgc tgtctatcat
    44761 gcagacggaa agcattttcc gaggccaggt gataaagttc gacttggtaa ggattttctt
    44821 tgattccttc ttctaacact gtttgtgctt cttcaatcat ttcttcttct tgaagagctt
    44881 gtcctagata tagatataac gattgataat gcggattaac ttctcgaagt tcttccagat
    44941 agttgattgc ctgctgattt tctttcaatt gaagattcgt aaaagctagc tggaacagtc
    45001 gatcatctgt cctttcttct ttcagtgctt cttctaaagg tacgatagct gcttcgaatt
    45061 ctccttgcat acttaaggat tgtcctaaac gttcctgcat cgagattcct gaaatttcat
    45121 ttatcccgct tgccaacaat gtttcataaa ccactgctgc ctctttgaaa cgatcaactg
    45181 aaaaatacaa ttcagctagc gcaaactgga tcaatggttc atcaggaagc aacccagctg
    45241 cttctttcaa ttttgcttca ctcacttcag ggattccgat aacttggtac aaatcagcta
    45301 tcgccaataa gctttgcgga tagtaatcac tttgcttatc cactttttcc aggtattcaa
    45361 aagctaagtc gatctcatta tcttcgattg caatttctgc taacgggatg ttcagtccgt
    45421 cattttctgg gtattggttg atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc
    45481 caatagccag caattcttcg cctagttcag ctaatatatc tgctgggtct tcttttaatg
    45541 ctttttctaa aaagatttgt gcttgtgaca agtcttcatc tgataaagct tgcaacattt
    45601 tttcgctatt tgtattcatt tcaattccct cttatctcac tgacgatttg ttgaaatacc
    45661 gtcattgttc tcaataattc tttttcgttt ggatgttggt atccgtctat tatacctatt
    45721 ttttctaaaa caagtatttt tttgcttttt tctgccaaaa aaagaacttt ttccaaatac
    45781 tccatctttg aaatctgttc cagccagtgg attggatagc caagcaagtc cagccacaca
    45841 aaaaagtttt ttagttggaa atcaaaagga tatgcttgga gattccacac gaaatgaaac
    45901 atcattccta aaaatttttt catactagca gaaagtagat ggccattttc ggtcagataa
    45961 aaagcttgtt caaacagttt accataattt gacaggtttt ctgcctctaa ttgataaaaa
    46021 tagatcattt cttcgatcat cccggcaaac ggccgagtcg ttaattgctt tttatttgga
    46081 tagtttttat acagattttg caaaaaacga tgatcacaaa tcattccgca acagataaaa
    46141 gtcaaaaagt caatctgctt tccgtccacc tgccgctcgc tgattgtttg atcataaacg
    46201 atgcgttctg gcagattgat ggtttgcagc actacacgat ttggtagctg caaaatcgtc
    46261 cgctgcggtg ttaatgctcg gcttagagaa cggatcgata cagggatcat ccatagtgat
    46321 gaatcaaggg ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca
    46381 aatccgatga taaacatgga ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc
    46441 tttaattctg ttaaatgatt gcattgagtg ttggcacaaa tataccagtc attgtttgct
    46501 tgatttgcaa ataacgggtt gatcttatct gcaaacaaat cataatagcg ctgattcgtt
    46561 aaaaataaga ttggctgatc aatggaaagt tctcttagct gactgactag cgtttctccg
    46621 taaaacactt tcgtcaacgc atcttctact tgataatcaa gttccatacg catccctcct
    46681 cttcttattt ttattttacc attagtaagc aaattctaga aaagataaga gcaaagctca
    46741 cctcataact tgcaaataaa taaaaattaa ataaaataca tgtgttttct atgtatgaca
    46801 tacggattcg gaatctctgt ctcttattta ttccttttct gctaggaaat aaaaagaaaa
    46861 gcacttgtag ccctagagcc acaagcgctt tttcaactgt cttatttaac agcgtctttt
    46921 aatgctttac ctggtttgaa tgcaggtact ttgctagctg gaatttcgat ttcttcacca
    46981 gtttgtgggt tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg
    47041 attaattgaa ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa tacagcatct
    47101 actgctgcag ttgcatcttt tttagttaaa tcagtagctg aagcaacttt ttcgattaat
    47161 tctgctttgt ttgccatgga tagattcacc tcctccacaa gagatatgat agaaatatca
    47221 tttagttaaa actttctgag tggtccagaa gtttaaaaag aaatattgat taacttcaat
    47281 attctgttat cacgatatca tatgaaccgc ttaatagcaa ggtttttacc aattttcaca
    47341 tgcttttttt agtcatagaa acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa
    47401 cgtcgatatg gtaaaatgcc tattttctcc tacgagggat aattcggatg gacgtccctt
    47461 caaaagtaaa tgcttttctg atttgatttt ctaagaaacg ttcataagaa aaatgcatca
    47521 attcctcttc attgacaaaa acgacaaatg taggtggttt gacagcgact tgtgtagcat
    47581 aaaaaatctt caggcgtttt cctttatctg ttggtgttgg attgattgct acagcatcca
    47641 taatgatatc attcaacaca gccgaagaga tacgaagatt ctggttcata cttacttgtt
    47701 cgatcatttc aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg
    47761 gagcgtaatc aagataccgg aattcatcac gaatctcttg ttcgaaatca cgcattgtat
    47821 tggtttcttt ctttacaaga tcccatttat tcacgacgat aataattcca cgaccagctt
    47881 catgggcgta acccgctact cgcttgtctt gttcccgaat cccttcttca gcattcagta
    47941 ccatcaaaac gatatcagaa cggtcaatgg cacgcattgc acgcatcacg ctgtattttt
    48001 cagtattttc gtatactttt ccgcgtttac gcataccagc tgtatctatc attaagaatt
    48061 tctgaccact ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat
    48121 ccgaaacaat tacacgttct tctcctaaaa tcgcattgat caaagaggat ttaccaacat
    48181 ttgggcgacc aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag
    48241 agaaatactt cactgcttca tctaaaatat caccaatccc tagtccatgg cttcctgaaa
    48301 caggataagg atcgcctagc cctaaggaat aaaattcata gatatcgttt ctcatttctg
    48361 gattatctac tttattgact gctaaaatca ctggtttatt acttctgtat aaaattttcg
    48421 caaccatctc atctgcgtct gtcacgcctt cgcgtccgct gactacacaa atgattacat
    48481 ctgcttcttc aatcgcgatt tctgcttgat gcttgatctg atccataaaa ggttcatcgc
    48541 caagatcaat tccgcctgtg tcgatcacgc tgaattctcg acctaaccat tcaccttttg
    48601 cataaatacg atcacgagtt actccaggag tatcttcgac aatcgagata cgctcaccag
    48661 cgatgcggtt aaaaatcgta gacttaccga cgttcgggcg gccaacgatt gcaattgttg
    48721 gatttgccat attatttcct cctcacaagt ttccaatctc acttagttta acgaatgcat
    48781 tgccaacttt caagcataga ttgtcaatta aacgaagtat ttttattcct tattgaaata
    48841 ctaatcgctt ttcaaaagaa aaactgagac aaaagcagct gctttcatct cagtcacaga
    48901 tacaaaatca attatttttc agaatcatcg gttgcttttt cttctacttc ttctttcaac
    48961 gcatcaccta aaatgtctcc catagtaaat ccagtatttt cttctggtaa ttcataagac
    49021 tcaacatttt ttggttcttc ttctgaagct ggtttttctt ctaaagcttt gatactcaat
    49081 gcaatacgat gttcttctgg atggatctct aatactttca ctttcacttc gtcaccttca
    49141 tgaagcactt catgtggtgt cgcaatatgt ttatgtgaaa tttgagagat atggactagc
    49201 ccttctactc cagggaacac ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt
    49261 ccgtctaata ctgtgccaac tggagctttt gtatcgattt ctgaccaagg tccaggctgt
    49321 gtgtctttga ttgacaagga aacacgttct tgttcaggat tgatagacaa cactttgaca
    49381 tttacatcat cattgacagc caatacatca cttggttttg ctacatgact atgtgaaatc
    49441 tctgagacat ggactagacc gtcaattccg cctaggtcga caaatgcgcc aaaatcagta
    49501 agacgtgcaa cacgtccact gagaacatct ccctcatgga tagttgaaag aatttctttt
    49561 ttcttttctt ctctttcgtt ttccactacc gctttatgcg ataagatcaa acgattttct
    49621 gatggttcga tttcgatgat tttaaagctc aatgttttgc ctttatagtc tgaaaagtct
    49681 gcaacaaaat gatcttccac cattgatgca gggacaaaac cacgtacacc gacgtcaacg
    49741 actaaaccgc ctttgacaac atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa
    49801 tctctttcga tatcttccca aactttttta gcatctaaac ggcgttttga aagtagatag
    49861 ctgccgtttt ctttatcttt gccgatcgta ctgataacga ctaaatctaa tacatcaccg
    49921 acttttacta cttcgctgat atcttctacc ggcaaagttg ataattcttt tgccggaacg
    49981 actccttcaa ctccagcccc ttcgattccg acaatcactt gtttgtcttc aatgactaaa
    50041 acttcgccct taacaacatc gccaactttt acttcttgta cgcttttcat tgcgtcttcc
    50101 attgactggt tgttctcttg attttgatca aattctgtca taacacttgt cctcctaacc
    50161 aacattctgc cgtaagtacg gtactgtttt tagtttaatt gattattcaa aaaaaataaa
    50221 gcgatttcgt tcgttttcct tgaaaaaaca attgtttttc attttttttc ttaatttaaa
    50281 aaagaagata accttttttc aaaacaacag cttttattgc tgcaacgact tctggaatcg
    50341 atttgccagt cgtatcgata cgaacagcat cctctgcttg cttcagcgga gagacgtccc
    50401 ttgttgaatc ataatggtct ctttcctgta tctctttcgt cagtgtttca agatcagtcg
    50461 gaattccttt ttcttggttc tctttgaaac gtctttctgc acgctcactt acgcttgcta
    50521 ctaaaaagat tttcacttct gcattcggta atacagctgt cccgatgtct cttccgtcca
    50581 tgacaacgcc accgcgttcc cctatttttc gttggacagc aactaattct tctctgactt
    50641 ttccatgtgc agagacttca gaaacagctt ttgtgacatc tggttgacgg atttctaaag
    50701 tcacatcttt tgatgctatg aatacatgct gtccatcttc ttgttgcgaa aaagagatag
    50761 gatacttttt gatcagcgaa actagccctt cttcatctga aaaagataca ttgttcgtaa
    50821 tagctaaata agttactgcg cgatacatgg caccggtatc tgtataaata taattcaatt
    50881 cttttgctaa tatcttggca acggtacttt tcccagatga agcaggcccg tcgattgcaa
    50941 tactgatttg tgtcatgata taactccttt ttctcagaaa aaaagccgca aagcgcggct
    51001 attattattt aattcttaat tcttgaccag ggtaaagcat aaagttgttt ggatctaagc
    51061 cattcaattc aaacaattga tcagtggtga tccctgcacg ctcagccact tgttttggac
    51121 cttcgcctgc ttgaacagtc gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt
    51181 ctgctgcatt gctgcttgaa tcccctgctt gataagttgc ctcacttgag gaagtcggtg
    51241 ttgtctctgc tgcaatactg ctttctgacg ttacacctgt atcttgagac acagtagatt
    51301 cttccgcgat tgtactagat tctactgttg aagtctcagt tgactcctct tttgttgtac
    51361 ttgtagaact ttctactact gatgtcgaag tttcagaaga agttgctgct gttgcattgt
    51421 tgcttgatcc atttctgatc cagaaataag cacctgctgg aatagcgata cataagacca
    51481 gtaaaatcaa aaatagcgaa agaaataatg tatttccttt cttttgttgt ctctgagaac
    51541 ttcttgatga aacttcatca tctgtatcgt agattggttg ttcccatggt tcttgtgttt
    51601 cgttattttc tgtagattga tgtctatctt ttctgctcac tgttttacct cctaaactct
    51661 atcgtgtgta ttataccata ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt
    51721 ttttttaaat aaatttagta aaatcgttcc ccaatgaagt tgttcagtct tagaaaaaag
    51781 cttcctcttt ggcagaagtt ctgcttttgc tccatcaata tcacaacaat tctgcggctt
    51841 ttcggagagt ttttcgccaa aataagctaa gatgaatttt ctgcggcagt tttcttcatt
    51901 aatatagcga agcatctgct gcaggcggaa ttctttttct ttttcctgac gtttgagccg
    51961 ttccacccaa ttttctggtt tttcagactg ctggatcagc tccatccatt ttttttgcag
    52021 ctcatcaaag ggagcttgct ctgtggcata ttccaaataa agctcaaaac tttgtcgctg
    52081 ttctctagaa agttgattaa agaaataatg gatacgttca tccccacttt gataaaaaag
    52141 aattgctgca ctttcttcct gatctctgcc tgctcgaccg atttcttgta catagttttc
    52201 tagactgtct ggcaaatcat aatggaccac ataacggata tcagatttat cgatccccat
    52261 accaaatgca ttcgtagcaa tgagaaactg cagctgattt tttacaaact gctgttgaag
    52321 ttgtcttctt tgagctgcat caagaccacc gtgatagtat ccaactgtaa aacgttccct
    52381 gaataaatga tacaatcgtt cgacttcttt ttttgttgca caataaatga ttgccgctcc
    52441 ttgtgctctt tccatgaagt gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa
    52501 aagcgagata ttttgccgat tgactgattg tctgatctct tttggtggtc gatcaaatag
    52561 tacatgacgt atatctcttg ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac
    52621 cgggtttcct aaaagctttc ggatttttcc taactgttga tattctggac ggaaatcaac
    52681 tccccattga gacacgcaat gagcctcatc tactacatat aacgcaatat tttgtttttt
    52741 cagatgctct aaaagagaag gattagtcaa catttcggga ctcaaaaaca aaaatttata
    52801 ttgcgataga tgacggagga cgtatcttct ttcagattgc gaaagtaaac tattgaaagc
    52861 agctactcgt ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag
    52921 cagcggtgac acgatgatga ccagaccttc cattaaatac cccgtcaatt gatagcaaag
    52981 gctttttccc gttcctgtag gcaaaatagc gagagtatct tctccatcaa gcaacgcctg
    53041 aataatttct tcttggccgg gacgaaatga atcaaatcca aaatattttt ttaattcatt
    53101 tattaacatt ctgtatcgct tcctttctca atagatagat ttgatacaaa cgaaattctc
    53161 cataatctaa ttgtccatct acattccatt cgctaaagcg ccactctcgc acggaatcct
    53221 tatttggttc caaacgttcg attgtttctg cagagagaat ccactcgtat ggaaaatcat
    53281 caaaaaacaa ggcccactca ataagatgat ctgttactgt tcctcttttt aaatgacgga
    53341 ttgccatgat ctggtcaatt gtctgaccat tcataaacat ctgcttggtg atcatcatgc
    53401 tttgattgaa attttgctgt aataatgaat cgatcaaagc gtgccaaagc gaatctggcc
    53461 gtttttcgat ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga taaaccgctg
    53521 actcatcatt gttttttgcc agctgataag gaagcagccc tgttcgctca tttccagata
    53581 attggttagc taaaaaatct gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac
    53641 tctcatacgc agtatcaatc aaaatttcac gtggcaattg gctttcacta agccattttt
    53701 ttagttggaa agtataaaaa ggacttgtct ctgctggaag atagtcttga atacccgttg
    53761 ccagattaga aattacttga acagcaaatt taatgagccg ccaactagtt tcccctgttc
    53821 tgccatagcg gtcaaaacgc aaaccagtat gttctatttg atgttcggac aacatatcag
    53881 cccctgcagg cgttagcttt gcttcatttt catttatttc gatccaacct tgctgcgcaa
    53941 ttttttgcaa aatttgataa aacttatcct gcttcaattc tggaaaactg ttatggataa
    54001 acaaaagttc gtgggtaaat ccataaataa gtactgaagt cgttcgtttt cccacaagga
    54061 gttggtaaag agaactaatc cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa
    54121 caatcatatt attctcctta agttaggatg gttagaaatg tcactactat gtaaacttgt
    54181 acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa gcgccagata ttttcgatta
    54241 tgatgaagaa ggaatcgttt tatttgccca agaaccagag gctcatcaac agttcgtgat
    54301 gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt
    54361 attagaaaat aaaatataaa tataaacgaa actttaaaaa cgtacactaa gttctctaaa
    54421 cttgaaaata agccgggatc tgtttctctt gttccgtgaa atcccgactt attttgaaag
    54481 attcatttgc tatctatagt taattgacta gttttttcac ggaccctata ggacccattt
    54541 atattatttc aactttttca gccagttttc caatgtattg acgatcacag tttgctggtc
    54601 tgcgttagaa atttctgcct ctccatctcc tttttgctca ccatagctgc caaaacctcc
    54661 atgatttccg ccattgattg atacatactc tgttctttcc ggtaaatact gcttagcttc
    54721 ttgataagct tcctgattca atactttatc ttttgtcgcc gtgatcgata aaacaggaac
    54781 agctgattct gtcaatgttc cttttttgtc gggataactt gccatgaaga ataccccttt
    54841 taaatgtttg tctgaatgat tcttattgac ataacgactt gccattacgc cacctaatga
    54901 atggccaccg atcacataat cggaatttgg aaaatcttct ttgaccttat ccgctttgtt
    54961 tccgcccatc actgctagat caagtggcat tttgacaata gcgactgaaa agccgttttt
    55021 tgctaaatct tgcgcccaaa tactataact ttctggctct acgagagcac caggataaaa
    55081 aataagtaat ggagactttc catgattctt tttctctcct tcaaaaaata agtaatcttc
    55141 tttgtttttt ccttctttta ccaacacttg cgcttctgac gatggttcgt acgtattttg
    55201 ctgtatatag taataaccgg ctccaccgag taatcctaaa atgatgacta agccaattag
    55261 tattcgtcta aatattttca ctttctccca ccttttctat ctgagtgacc cctttttctt
    55321 aatcaagtat gattatacct taaaaaaata agagagaaat gcaaactttc cagcatcaaa
    55381 taaaaaaaga cggaatatcc atttgaatat ccgccctttt tgtatgttta tttttattta
    55441 gcacataaac gatacacagc ttctgcataa atttccattg ctttatacat actacttaat
    55501 tcccatcgtt cattcgcctg gtgcataaag tcaggagtat cagggaacat cgcaccgaat
    55561 gccacacact ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc
    55621 atgtctccag taatatcgcg atatgtcccc aatagatttt tcactaactc actgtctagt
    55681 ggcacgtata atgaatcaag gaagtcaaat tcttcataag tcaacccata tttttctgct
    55741 gttttagtta atttagctac taaatcatct ttcttgaatg taacagggat tctcatatct
    55801 acaccgattt ttgtttgttc tggtgtgatt tccaaacttg caaagttcat cgttagctcg
    55861 ccggattgtt catcttctgt tttgcccacg acagcttcac cagttgcatt ttcttgaact
    55921 aattttccta agaaattgat tgggccaaaa tcaaatactt ctgaaagagc aattgccaga
    55981 cgagaaatag cattcactcc ttgtgctgca tctttcgcgt ggatactttt accaagaacg
    56041 acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact
    56101 tcagaaagct tttctccaga gtaaggagct gcatcaggaa caacattcaa cgctcctcca
    56161 gcttttacag aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa taagcctttt
    56221 tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct
    56281 ttttcgttat atttttctaa acaacgccat aaattttctt catctgtacc aaagataaaa
    56341 cgaatacgtg tcttgaattc cacgccagca tcaatcaaag ctttgacggc atacattgcc
    56401 gcaatagaag gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc gtcttttata
    56461 gttggatcga atggttttgt ttcccaatta ttttcgtccc ctgctggaac aacgtccata
    56521 tgacacaaga taccgaataa ttcatctcca gagccaatct ctgaataacc gtaatagcct
    56581 tctggatctt taaatgttct aaatcctagc ttttcactga tttccaatac tttatccaaa
    56641 gcttcaatca cttttttacc gaagggatgt ccttgtcctg aatctgcttc atctagtacg
    56701 gaaggaattc ggatcaattc gctcaatgat tctaatgctt gttcatgatg ttgttctgtc
    56761 acaaattttt tcatgatatt atcctcctaa tatgttaaaa agggtcatgt caaattcctc
    56821 cgacacaacc tcttcttctc aaataatgac gttttaagcc tgctccatca gcaagcagaa
    56881 tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat ggagctattg acttatccac
    56941 gtgcttatcg atagccgttt atcggtttct tcattcttat gatagacctt gtacctgtat
    57001 ttgtctaggt ttagaacatt gctgctacac ctaggatcac acagatggct acaaataata
    57061 tcgcaattaa tttaccggta aatttccacc acactgaaac atcgaaacga gcaattgcta
    57121 cagcacccat tacgatagca gatgttggtg taattaagtt tactaaacca gatgcagatt
    57181 gataagcagt gattactaaa tccttaccga cgccggcaaa ttcacccata ggtcccatga
    57241 ttcccattgt tgcagcagct aagccggaag ttgaaggaat caagaatgac atagggatat
    57301 agaaaataaa tgtaataatg atgaatacac ttgaagataa tgaacttaat ccttgttcgc
    57361 cccaatgtaa gatggtgtct gtgattaatc cattgttcat aaccacttgg ataccacgag
    57421 caacagctac tacgattgct acaccgatca aatcagaagc accagctaaa aattcagaga
    57481 caaattcaga ctctttcatg ccataagtga ttgctactac gatggacatt actaagaata
    57541 acatagtaat ttctgggaag taccagtcac ctaatggaag catgctttta cctagaaccg
    57601 tacctagtac cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg
    57661 cattcaaggt tgtccaagga atcaaactga tgatcatgat accaaaagta ataaagaaca
    57721 atacattaac atgtttttgt tttttcgtga ttacttcttg acgaccgatt gcttcaatat
    57781 cgaaatgctt catatcttct ttacgttggt tgtatactaa tgattttgtt ggatcttttt
    57841 cgatctttga tgcataacga tacacatata caatagaaat agctaacaaa ataacgaaca
    57901 tcaagaatcg aagaccaata ccttcaccca tcgaaatccc aacagcttgt gaagctacgc
    57961 ctgttgcaaa aggatttacc gttgaagcta gacagcctac ttgagaccct actaatacga
    58021 ttgctacagc tactactgta tcaaagccta cagccatcat gactgggatc agtaaagcat
    58081 agaatggaat cgtttcttct gccattccgt aagtagagcc gcctaatgca aataacagca
    58141 tcaatactgg aataagcatt ttttcttttc ctttgaattt cttcacaact gaagcaatcc
    58201 cggcatctag tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca accaaaatga
    58261 agaaagagat tgggattgca gcaggtgttt ctttagttag ctcccccgct ggtacaccta
    58321 gcatcccatt gattggcgcc atgaaaatat cccacagccc ttgaggattt tgttgtactc
    58381 gttcatatga tccagcgacg atgttgcctg catcgtctac actatagtgc ccagctggta
    58441 taaaccaagt aagtacagcg attactgcaa taataataaa taagaccgta tacgctgaag
    58501 gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt cttcctttct tttccgttag
    58561 agacaacaat tttggctcta atgttatttt atcaaaaaga gtatcagaac aaaaataata
    58621 acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc ctcctctttt
    58681 ttcaggtttt taaagctttc ttctttttca ttgtagcgtt tccttttttt tataaacaaa
    58741 cattttcgtc ttttaaaagg agaagaattt cacatttgca aagaattgat tgatttttat
    58801 gcataaatca aaaaaatact caaaacaaac aaataaaacg tttacattat tgcatgtctt
    58861 gtattttttt ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac
    58921 tgctgtatga cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc
    58981 atgcagcact aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat
    59041 cccaatcagt acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa
    59101 aaacattaag taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag
    59161 agcgatggtt cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac
    59221 actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc
    59281 tcctaccaaa ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa
    59341 cgctgttgca actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc
    59401 actaaaatcg atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa
    59461 aactaatgcg atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt
    59521 catttgaaat cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa
    59581 tgaaaaaact ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa
    59641 ataagctcta tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc
    59701 tatttttttg caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc
    59761 tgaagacgaa ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa
    59821 cgcaacatct aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg
    59881 acttcttttt tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct
    59941 ccgtattttt cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc
    60001 acttgatgat tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga
    60061 tccactgata aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct
    60121 tttgtcaaag gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact
    60181 ttatccactt catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt
    60241 agcaagatcc ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc
    60301 atcggcaaaa gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact
    60361 cctttaggtt tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc
    60421 acttccacag tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt
    60481 acttttacac gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg
    60541 tgggcaatta ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa
    60601 agaatagatc tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca
    60661 gttcttctaa tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata
    60721 tcggccgacc tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac
    60781 gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg
    60841 gctgtttata agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg
    60901 tcccttgcgc atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat
    60961 ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta
    61021 attctttcac taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt
    61081 cattactgat cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg
    61141 tcatgttcgg tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt
    61201 tgcgcaatga tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact
    61261 tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt
    61321 atacgctctt cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt
    61381 ttttccactt tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa
    61441 tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg
    61501 ttcataggtt cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca
    61561 gctgcatatt tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct
    61621 tcttcccata attcttcatc gtctgtgacg aattcttgga caggaagcaa tgtcttgctt
    61681 ttgattgcca tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt
    61741 gttttcattg tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca
    61801 tatatatcga tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat
    61861 acatctaatt taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta
    61921 gccattttcc aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact
    61981 tttcctgtaa ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca
    62041 aagaaatgtc atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt
    62101 gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt
    62161 acgtatcaat ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata
    62221 gtcccatagc aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct
    62281 atgactaagc ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat
    62341 gtgtataaat ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca
    62401 aatcagctcc attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta
    62461 cttctttgta aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc
    62521 gagaaagacc tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa
    62581 gtaatggacg tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag
    62641 gaatgatccg ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa
    62701 gatctcccaa tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt
    62761 ccaagattgc acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct
    62821 tttctacttc tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga
    62881 tatgttgcat cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc
    62941 tgagacttga gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct
    63001 ccagaaaacg gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct
    63061 gtagaaaatg caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca
    63121 atccccgttc aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc
    63181 ccctcatgcc tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc
    63241 tgagcaaaga aaaagccgtc tagacactca gacggactgg tttctttttt ctttttcata
    63301 gcattcctga cagattccat ggaacgttag ccgatgatct tttactaaaa aatgataacg
    63361 ttcttctact acttgctcca cttcaagtaa aagatcctct tctacttcct cgatcgtgcc
    63421 gcagttcaag catagtaaat gatgatggaa atgtttggcg ccttcttttc tcaaatcata
    63481 acgggctaat ccatcattaa aacttacttt atcgacaatt ttcaattctg tcaagatttc
    63541 aagggttcga taaacagtag caagtccaat ttccggactc ttttttttca ctaaaaagaa
    63601 gatttcttct gcagataaat gctctttctc attttccaaa agcacaagca gagtagcttc
    63661 tctttgcggt gtcagcttaa accccgcttc atgtagctgc tgttttgttt ttttcatcgc
    63721 cgaaatagca ttcatgacca tttactcaac cccaacaatt tataataatt acaaataaaa
    63781 aaagatataa cactatgtta attaaaatta ttataagcta gttaagaaaa acatgcaagg
    63841 gattctcaat tagatttttt gactagtttt gtcacaccgt tttcttgtgt aatcaatcct
    63901 tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt tgctgatacc aaaagctttc
    63961 atgatttctt ctggatccga tttatctgta aagttgattt gctgatctgc agaacgttct
    64021 aaaatggtta aaatcatttg tgcatcatct gatatagctt catatgctct tggcttcaat
    64081 gaaagattca gtacaccgtc tggtcggaca ccaatcactc gaccttttat ctgctcgcct
    64141 agacgaggtt cattatatcg ttcagaagga tggataaacc caagatagaa atcttccgtt
    64201 aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc ctatcacatc tttgtttttc
    64261 atttcttctg ttccatattt gcttaaagat ttgaatattt tttcatctgc taaactgccc
    64321 caaatacggt cttttgcatc aactctaagg gaaatcatca agcgatcccc ttttttaggc
    64381 catagctctg tcattgtcgg caattcatca agagatacga caaagtcttt atctggaaga
    64441 ccgatatcga caaacgcacc taaatctttt cgtgtagcag taacgattcc aaaagcgtaa
    64501 tgaccgatac ggcttttagg aatttgtgta gtgatccgat tttcatgttt ttgattttgg
    64561 taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac cttctgattt gcttaagcca
    64621 aaggtttgtc cgtttttttg gacaaagtag tttttttcat tttcatcaat aataatacct
    64681 gtaaatatag ttgctaataa atcgttcatt tttttctccc tctatagtga aaggttttct
    64741 taatacattt ctttgctatc ataacataat gttatctact tgaatagctg agccttggtt
    64801 tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat actccccttt ttcgaataaa
    64861 cggtgttaaa cggcaggctc acagcctctt tcatttgcct aaaattaatt gctcccattt
    64921 cctccgaacc atccagtttt acagttgatg gttacgatgt aaaatggcaa agcactttct
    64981 ccaatgcctt tcatcaatgc agattttgtt gaagtggcat atagattata atagccttcc
    65041 ccattttcga ttctggcttt ttctaatctg taacctcctt caacgatata tccacgttgg
    65101 atacatgtag caatcacttt gctttggaca cctggtgccc acgtccatgc tggattagat
    65161 gcatcggcaa tatcacttgc tctggcagat ttgattggct gattcgctga gtttgaagag
    65221 gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg
    65281 gcttgagctg cagctgctgc ttgagcggct tgttttgctt gttcttcagc tgcttttttc
    65341 gcttcttctg cttttttctt ttcctcttct tcttttttct ctttatcagt gagcatctta
    65401 tctacttgtt tcaattgttc attcaactga gcagatagat ctgtattttt cactttttct
    65461 actgcttctt ttgccgtctt gtattggtcg cgtgttgctg aatccactac ttttccgtct
    65521 ttaaagacta tagctgtttt ttctttagct gtttgcaatt cagcatattg atttttagct
    65581 tctgtttgtg cttgttgaat caattttcca aaatctgtgt tatccgtttc gattgcttgg
    65641 atctctttat ctgctttcaa cttgatgtca tctgctagat gatcatcgac gatggcaggt
    65701 gctatgaacc actcattcac tttttggata tctcggatct tagattggat atcttggtaa
    65761 gttgtttcta actctgaata tcctttttca ttcttaagac tggataaatc attttttagt
    65821 tttgataaat cttgatgaat catatctgca cgaataaatt gatggtgatc atctgtataa
    65881 aaggcagcaa gtttctcatg aagttcttcc atttgttgct gtctttttgc ttctgctgct
    65941 acttcggctt ctactttttg atgatggtca taataagccc agcttcctcc accgagtaga
    66001 agtactgctg ctaaaacgag ataaggtact ttttttgatt ttttcttttg cgctttatct
    66061 ggatctgttt tttctaagtt tgattcttga tgtgtttttt cagtatctgg cacgattgtt
    66121 attgatttcg cttctgcttc aaacagctta ggctggttct cttctttttc tatttttgct
    66181 tcaagatcag taccctcttt gcttacagat tgattttcag catcggattc actatttatt
    66241 tcatcgggct ctgttgactt tttgtctgtg ttttcgagta tttctttttt ctctgcttcc
    66301 ggcagtttat tcgttttttc cttttctaat tgtttttcag taaatacttc agttttttct
    66361 gtgatatccg tttttacgtc ttttattttt actgtttctt tacctgtggc gttttctcta
    66421 tcagagtctt tttcagtttc ttgttccttt tctggctcta ctttgttttc ctgtgcaatt
    66481 ttttcactga tcattttgag cagctctttc gcgtgctctt cttttgtatc ttcacgatgt
    66541 gctttgatat aagcggatag tatcgaattt tcttcctcat tatcgacgat ttctttcagt
    66601 tcagctactc ttcttttttg ttttgattca agcgtttctt cttgattctt tcctgattct
    66661 tccgaagtct catgaacagt tgctttcttc tgttcatttt tctctatatt agttacttga
    66721 tcgtgagcag gtggttcttc gtcttctggt tctgcttttt cgaataattc cattacagat
    66781 tctagaggta aatctttcaa ttccgaccat tcgattggat cgtttaattc tgtatcagga
    66841 aagactgcat cttcagttga tttatcctgc atagctgcat ctgtatttgt ttcttcaatt
    66901 gattctttta tttgctgatc ttctgcagtt atttgatgac cgcagtctgg acagatagta
    66961 ttctgctcca tttttttcga tccgcaattt ggacattttt tcatcactta gcgatcggct
    67021 cctttactag gtttacgcta ccatttaatc tatcatattc tatacgtcac gccaatagct
    67081 tatttttagg atttacttaa aaataatgaa tgaatttcct tcctatagaa aaaaaagctt
    67141 gagactgcgt agtctcaagc tttttttctt ttaaaataaa ccaaacagtt ttactgattt
    67201 ctcttctggt caatagtcca cgcttattct aagaaatctt ttaattgttt tgaacgagaa
    67261 ggatggcgca attttcttaa tgctttagcc tcgatctgac gaatacgttc ccgagtaaca
    67321 ccaaatactt tccctacttc ttccaatgtt ctcgtccggc catcatctaa accaaaacgt
    67381 aagcgaagga cattctcttc ccgatcagtc aatgtatcta gtacatcttc caattgttct
    67441 tttagtaact cataagctgc gtgttctgct ggacttgttg cttcctggtc ttctatgaaa
    67501 tcacctaagt gagagtcatc ttcttcaccg attggcgttt caagggaaac aggttcttgc
    67561 gcgattttca agatttctcg gactttttct gttggaagat ccatttccgc accgatttct
    67621 tctggcgtag gttctcttcc aaggtcttgc agcaattgac gttggatacg tatcaattta
    67681 ttaattgttt caaccatatg aactggaata cggatcgtac gtgcttggtc agcgattgct
    67741 cgagtgatgg cttgacgaat ccaccatgta gcataggttg aaaacttaaa tcctttgcgg
    67801 tagtcaaatt tttcaactgc cttcatcagt cccatgtttc cttcttggat caagtcaagg
    67861 aactgcatgc ctcgtcctac ataacgttta gcgatactta ctactaaacg aaggtttgct
    67921 tctgccagac gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc taacgctact
    67981 tcttcttccg cagttaaaag ggagacacgg ccaatttctt tcagatacat acgaaccggg
    68041 tcattgattt ttacaccggt tggcgcagaa agatcttctg tttttgcttg ttcagctttt
    68101 ttctcagcac tcttcaagct gtgaattgat ggatcaccat tttcatccac cacactgata
    68161 cctgcgtctt caatcttttg gatcaatttg tccatttcat ctgcattcaa agtgaatggt
    68221 gtagcaagct gattggataa atcatcatat acaacttgtc gtttgggttt gttttctttg
    68281 atgaaagctg ctactgctgc ttcatacgtt tttttatctg ttccatttgc cataaaagaa
    68341 ggcccccctt gagtagctat ttggcttgtt ttaactgttt cgttaaattg atgatttcaa
    68401 tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt
    68461 tttttaatgt tatttcatca gctaagccag atttgctgat cacatctagc aagtcttgga
    68521 tttctcgtgg cgaactttct tctgccaccg gtaaatattc gatttccacc accaaccgcc
    68581 ttaaattttc atcttgcaaa aagtctaaaa actctgcaag gttgaattca taatgttgtg
    68641 agacatatgc atcgaataaa aaataaactt ctgcatatgc atcatgaata aactgaaatt
    68701 ctcgttcttt taaaatatta cggacagaaa cttctttaaa tgcacgatat aatagaatct
    68761 gctctgcctt ttctacttgc gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa
    68821 cctgagtgtg ttgactttcc tgaggttctt gacgattttg ctgatttgtc cttcgaagat
    68881 cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa ctctgttgac agttgagata
    68941 gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt aattcttcga
    69001 ggtattcgat cttgtctttt tcattatcta aatttttccc ttgttttaaa taacgtgatt
    69061 taaaagtaaa gactgtttcg cgcccatgag ccactaattc ttggaaagat gcttctccat
    69121 acttacggag atattcatca ggatcaagct tttctggaac gactacgaca gaaagtgcta
    69181 atcgactgtt ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt
    69241 ctccgtcata gcaaaagacc aattctttcg tcagcttttc catttgttgg atctgttgag
    69301 ctgttagact tgtccccata gaagctaatc cagttttgat accagcctgc catgcagcaa
    69361 tcacatccat aaacccttcg aacaacaaaa tttctccgtt tttccggata tcgcttcttg
    69421 ctttatcaag attaaataaa acttgtcttt tattgaacag ttctgtttct ggactattca
    69481 agtatttggg ctgatctttt tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac
    69541 cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac tctttgataa aaacgatcag
    69601 acatacggcc atcatcatgc gtgacaaaca atccagagcg ttctaacaat tcatttgaaa
    69661 ttgattcatt cttgaatact tgctgaagaa aagaacgctc attaggtgca aaacctatgt
    69721 tgaattcttc aattaattct aaagacaacc cacgactttg caaatattct aaagctggct
    69781 gtccagcttt tgtatgcaat agcatgtgat gatagatttc cgctgctttt tcatgagcag
    69841 caatcagttt ccccgtagca gattcaggat ttcggacagc tatagtacgc tgacgccatt
    69901 gatcttctaa cggtatctgt tcgaagtctg ccacttttac cactgcttct ggaaaactta
    69961 gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat
    70021 gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt acgttcttca tgaaaaggac
    70081 acagccccaa gtaattttta ccagactttt tcaactggac atattgtcca atcacctcaa
    70141 cgatattcgt ttgactgcgt actgtttcga taacctcttg aggaatccgt tgagccattt
    70201 tttcaccccc tgctctattg atatcttttg ggtttaacag aagatatttc aggagaataa
    70261 ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta aaggacacaa ttatacatgt
    70321 ttgattgtat catatttcat tgctttttca agtataacac atttctatct cattaagcat
    70381 caatttgcta ctcttttcag taactgattt tgaatatcag ccttccagta caatactcga
    70441 agaacaatat ttacgagcat gaatacaaag aagtatccta cgctctctcc caaccctaag
    70501 tttacttcca aaattccatg gaacagcatt gcagctacat atatccctac gatcgtcgtt
    70561 actgctagga agatgcgcaa tgggttgaag ggataacaag cttttacaac tgccatgcaa
    70621 ctgatcccaa tcagcaagta atacatcaaa gtagtagttt cagcatttcc ccatgcgaat
    70681 gtttttccaa gtagatacac cgcaatgata ttcactacta caagcaaggc atttggtaaa
    70741 gcattaataa gtgctgtagg caaataacgg taagtgattt ttcttttatc ttgttcaaaa
    70801 gataaaaaga aggaaggata cccttcaatc gctaaatcaa tcagcgtgat ttggataggg
    70861 ataaacggaa atccaattgc tgtaacggca catataatcg ataaaataaa tgaataaatc
    70921 gttttgataa aaaagatgcc tgagactttc gtcacattgt tgaccactcg gcgtccttca
    70981 aacaggactt ctggcaatgt agtaaaatca gagtccaaca ataccagatt cgcaatctga
    71041 cgtgttgctc catcaccttc tgccatagca atgctgcaat ctgcttctct taacgccaaa
    71101 acatcattca caccgtcgcc tgtcattgca accgttctgc cgctttcttt caattcattt
    71161 accagcaatt tcttttgctg cggactcact cgtccaaaaa ccgtatagcg atgagcagct
    71221 tctcgcactt ccatttcttc agtaatttga gaaagatcga catacgcatc ataattcggt
    71281 aatcctgcac gtctagcgat attggagaca gtaaaaggat tgtctccaga aatcactttc
    71341 agatccacac cttcctcatg aagataagct agtgtttctt gagcgttttt tcggattgga
    71401 tcatcgattt caaacagagc aaccggctca acaaatgctg gtttttcttc gttcaattgc
    71461 tgatttggag cgatccccaa cataagtacg cgatagccat tcgcttgggc tgtttttaga
    71521 ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta cacgactccc
    71581 agttctggaa attccatcgc gccccatttg cgatcagaag aaaatgcgac tttgtttgtc
    71641 acatcatacc gattagaaag ctcaaaataa tctctcatag cccgcatagt gatgttgtta
    71701 tcactgcttt ctgccagata cgtccccata atttctggga tggctttctc gtacagcgga
    71761 tgtagtgctt ctacccgctg gatactcatt ttcccttcag taatggtccc tgttttatcc
    71821 aagcataatg tatcgacatg ggccagcgtt tcaattgaat acatgtcttg aacaaggatt
    71881 cgtttttttg ccagtttggt tacccctgtc gttaatgcaa tacttattag caataccaaa
    71941 ccctttggaa gcatgcctaa caaagctgct gcagatgcaa taacagaaat tttgatagaa
    72001 gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa
    72061 ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt cagaacgaat agctttgtga
    72121 gtctttgcct cgctagtcaa gcggacagca taattatctg cacctactcg gataacttca
    72181 gccatgacag aaccgcttgt caaaaagctt ccagaaagca attcagctcc agtttctttg
    72241 acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg tccagatata
    72301 acctgtaaat cggctggtat ctgttctcct gctccgattt ttaccaaatc tcctaatacc
    72361 agctctttag tagaaatcga ttgttcttta ccgtcccgga tcactagtac ttctgattgg
    72421 gaaacaatcg ataatttccg tacaagattt cttgcatgta tttcttgata aattccaata
    72481 acgatattga cgacaataat tgctaaatac gccatgttgg aatacgcacc tacaaataac
    72541 agacaaatcc caataccaaa gttcagaaaa ttgaataacg tcaatatatt atcgctgaat
    72601 atttgttttg tactttttga tgcatcttcc tggtagtcat tttgctggcc agaagccatt
    72661 ttctgaacta cctcttgctg tgtaagtcca ttcattttct ttttctccat tttccgccaa
    72721 ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca agattaaaaa caacttgttt
    72781 ttataaaatt tatagaaaaa cacacgaatg attttacaaa aaagatgcca acctttttgt
    72841 ttgaatcacc cgtgtgctgt ttttattatc aatctatctt tacttatatt gttcctccaa
    72901 acggctcatc caccaaccta gtgctgtacc ggctataaaa agaacaggac aagctagtac
    72961 cccagcaact cccaatcccg cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac
    73021 tacacctaaa atgaaatgga atagcttgct atatgccttt gagaaaatgt aatccatgat
    73081 ttttgacaat ccaagaacac aaagaatccc accaattccg atcggcacaa tcacttcaag
    73141 tcgtacttct ttaaatccgt cagccatcgc tttatacaat cccatataca ccaaaaaatt
    73201 agaagggctc aatcctggga cgatcatacc caaaccaatc aatccgccgg caatcagcca
    73261 tgttccaaaa ttctgatcga cttgcccgtc aaataattga gcgccgtata ataaaaacaa
    73321 ataagccaga acaaaagtaa caaccatgat gatcacatca gaagtatctc ttcctttttt
    73381 ccctgcttct ttccataacg caggaacagt tccagcgata catccgacaa aaaaccataa
    73441 tatcgttgtt tcatacgcac ctaataaaaa actgactaaa aaagaaagta gaaagatgcc
    73501 tgtcactccg cctagaccaa caggcagaaa aaacaaaaca ttttctttga aattttttgt
    73561 aaggtgggct aaaaaagaaa tcagccgctc atacatgcca aagacagctg ctaatgcacc
    73621 accgctgact cctggcaaga taaatcctga accgataaac attcccttca caaaccgtaa
    73681 catccagtct ttggttgtgt accccacttc ttgattttcc attttttgcc ttttttccac
    73741 tatttgcgta ctccttcttt catttaattt tattcatgat tgaacatttc ctgctatttt
    73801 acttaaagac tttttcatag gtctctatta ttttttcatt cattttgaca atatcaggat
    73861 aaaacatgtt acctccatta tgatagagat agccgccatt gtaaagcaag gactggatcc
    73921 gccaatatcg gtattgtttc ccttcctcat ttcctaaaag tgggctcagc acatctttag
    73981 aatacgcttc tgctaattct actgtgtttt ctccgccatg attttttaca taggcaatat
    74041 aatctttccc aaaattatat gcttggacag ctgtccataa atcgcatcct tgtttctttg
    74101 cttctactat catttccgcc aaatatttga caccttgatc aatactgtca gctggattat
    74161 caatgatctc agcttctcca tgcagacttt ccgagctctg catgagatct gtcccttttc
    74221 cttttgattc ggtcaatatt atcgccttga tcaaattctc gtagttttcc atatgataat
    74281 tttttgcagc tgttgttatt tctatttgat agctttccac tcgttttacc gaacgataag
    74341 ttcttcctca taaaaacaac accgcaccaa taaccgtcag aatgatcatg taaaaaagaa
    74401 tacggataat agataatcgt tttcgcttca tttcatcacc tagttatttt gacatctttt
    74461 tttatcttac tatttcttta tcttcttggc tatttcttcc ttgatttctg tgtacgcctt
    74521 aagaaaaaat gaattttcct gtattttttg acatcttacg aaaatgatcg ttgtttttca
    74581 gtaaaaacaa ttgcccaatg actgatataa tcgttatact ttacacagta ggtgattatg
    74641 tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga
    74701 gcggtcaagg cttccctctc tttttactgc atggcaatga cggcagcggc cgcttttttt
    74761 cagaacaggt ccctgtactc gaacgctact ataccgtgta tctagtagat agccgaggac
    74821 atggtcgttc gacaaacgaa gcatctatgt tgaattttca gttaatggcc gaggatctga
    74881 atacgatcat gttgctggaa aaaatcgatc aggcagattt tcttggattt agtgatggag
    74941 caaatctagc actggtattt gccagcagct tccctaaaaa agttcatcga ttgattttaa
    75001 attctggaaa cacgcttgtt aaaggcgttc gtttttctgc cagagtcatc agtaatattc
    75061 attatgcatg ggtatggtta ctctctttat ttcgcccaag tcttcgcaaa aatcttttag
    75121 ttatcaaact tttgctgcat gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact
    75181 cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata
    75241 tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa agaacaagga catgaattag
    75301 ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca atttctttct gaaacataac
    75361 tattaggaat agaaggtgat ttatttgtta aaaaatacca tacaatggct gaaaaaccac
    75421 ctaggactgt tcaaaacaat ctttttgatt tctgtcattg tcattattgt cggtgaattg
    75481 atgtccatcg gtaaaactct atctatccag caattaggag aaacgtttgc cactatacct
    75541 gtatggaaaa gcggactaat gctattgatt ggtcttatct ctgtgttacc tatggttggt
    75601 tatgatatta ttttgaatcg gatgcttgaa caaaagcaga atcctcgtta cctgtttgag
    75661 acaagctggc tgatcaatac gatcaacaat attgccggtt tcggcgggtt tgttagtgtg
    75721 gggcttcgct ctgaactata tggacaaaag aaagagagca gaaacgtgat ccaagcatta
    75781 tcgaaagttt tcttgttttt gatggctgga ctttctattt acagtctgct ttctttttta
    75841 cttgtagtct tcacgccagt tgctcctttt ttgaagcaat attggatatg gttgatcggc
    75901 ggcggtctat attttcctgt cgtcttttta tttactactt tcaaaaagaa aggattcctt
    75961 ggtggtacct cacttaaaac tcaaggacag ctattgctag tttcattgtt ggaatggacc
    76021 ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg aaatccggat tgatctgtgg
    76081 caaacaattc cattattcat tgctgcttca gtgataggaa tcgtgtctat gattccagga
    76141 gaaattggga gtttcgatgt aatgatgatt atcgggttgt ccgctattgg tgtgccaaga
    76201 gaaatagtcg ttgtgtggat cttgctctat cgcctgttct actacatcat tccgtttttg
    76261 attggaatcg tctttttctt caaaaatatc ggttccactt ttgatcagcg ttattctggt
    76321 atccctaaac aattagcaac agaaattgcg cataaaatcg tggttgtctt attatacttt
    76381 tctggaatca tgctagtact ttctgccact atcccgcaag cttttacaga gtttcgctgg
    76441 cttcatagtt tgaacccatt gaaatttcat tttattatcc aatttcctag tatactattg
    76501 ggatttcttt taatcattat gggcagaggg atcgcagcac gtgtgaaacg agcatatttg
    76561 cctacaatct tcttgattgt tctagcttta ctttatgttt tattaagtga ttttagtttc
    76621 acaccggtgg ttttcttgtc tattctgctt ctgatcattc ttgcttctaa aaacgaatta
    76681 tttcgggaac aattgatcta ttcatgggag tggcgtacga tcgatggaat cattattggt
    76741 gcactgtcct tgctttacat cgttattggg gtatacaatc tgccggattt ccctcatcgg
    76801 cgtcaccatt ttatttcctt tttcttattt ccttctgaaa agatttggtt ttcgggattg
    76861 ttggccatca tcgctgttag tttcatgatc gttctatttg ttcatttcct tcaaggagaa
    76921 aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc tgaagatcct tacaacttat
    76981 ggaggaaact ccgatagtca attgatcttc ttaaaagata agcggatgtt tgcctatgaa
    77041 aaagacggtg aaccaactgt tcttctccaa ttcgcttgct tcaataataa atgtattgta
    77101 atgggtgatc cttctggtaa gaaagaagat tttccagaag caatcgaagc ttttatcgaa
    77161 gaaacagatc gactatgcta tttaccagtt ttctacgaaa caagcgaaga gattgttatg
    77221 attttgcatg aattcggcta cgatttcatt aagatggggg aagaagctta tgtagacctg
    77281 aatagtttta caacttcagg gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc
    77341 gaacgagaag gatttacatt tgatgtctta caaccaccat tttctactga gcaaatgagt
    77401 actttcaaaa atatctctga taattggttg ggttcacgaa aggaaaaagg gttttcttta
    77461 ggctttttct ctgaagatta tctacaaaga gcgcctattg cagttgtcaa aaatacccaa
    77521 gaagaaattg tcgcttttgc aacgatcatg cctacttaca cgaacaataa agtaggaacg
    77581 attgatctga tgcgatatga ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc
    77641 ttgcatttgt ttgcctatat gaaagaagaa catatccaat ggttcaattt agggatggcc
    77701 cctctttcaa atgtcggaac ttccagaaag agttttttac aggaaaggat tgcatacttg
    77761 gtttacgagt ttggttctca tttttattct ttccatggat taagagaata taaaagcaag
    77821 tacgctacaa actgggtctc acgttatact ctatattcta gagatagttg gattgcatat
    77881 gttatgattg ctttgctgat tattgataat gcgccagttg agcagacaca aggtagaata
    77941 agtggtttga aaaaatggat acgaagaaag tattaaaaac tgcataccga tagatgaaca
    78001 ctctcatgat gaataaaaga gactgaagta aaacgaaagt tcatgcttct acaatctctt
    78061 cttatacaat tgaagaacaa agagcggtcc aaacagaaat cctgtttgga ccgctctttt
    78121 attttaaaca tttttcatta ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg
    78181 taacgatacc tgaacctact gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt
    78241 caacagcgat tggatggatc aagtcaacat caatcgtcac gttgtcacca ggcatgacca
    78301 tttctgtatc ttctggcaat gtgattgttc ctgtaacatc agttgtgcgg aagtagaact
    78361 gtggacgata gttgttgaag aatggagtat gacgtccacc ttcttctttt gtcaacacgt
    78421 acacttctgc tttgaatttt gtatgtggag taatggaacc tggtttagca agtacttgtc
    78481 cacgttcgat atcgtcacgt tggatccccc gtaacaacac gcctacgtta tccccagctt
    78541 ccccgtaatc taacgtttta cggaacattt ctacacctgt tacaactgct ttttgtgttt
    78601 caggtttgat ccctacgatc tctacctcat caccgacacg aacagcacca cgatcaatac
    78661 gacctgaggc aacagttcct cgacctgtaa ttgagaagac gtcttccact ggcaatagta
    78721 atggtttatc tgtatcacgt tctggtgttg ggatatattc atctactgta tccatcaatt
    78781 ccataatagc agcttcagca tctggatcgc cttgcaatgc ttttaatgcg gaacctttga
    78841 tcacaggagt gtcatcgcct gggaaaccat attcgctcaa taactcgcga acttccattt
    78901 ctaccaaatc gatcaattct tcatcatcga ctaaatcaac tttgttcaag aaaacaatca
    78961 ggtattttac accaacttgg cgtgacaata aaatgtgttc acgtgtttga ggcatcggac
    79021 cgtcagttgc agaaacaact aagattgcac catccatttg tgctgcccct gtaatcatgt
    79081 ttttcacata gtccgcgtgt cctggtgcat caatatgcgc gtagtggcgt ttttctgttt
    79141 catattctac gtgtgcggta ttgatcgtaa tcccacgttc acgttcttct ggagctgcat
    79201 caatactagc atagtcttga ggatttgcta ggcctttttt gcctaatact gtagtgatcg
    79261 cagcagttaa agtagttttc ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg
    79321 gtttacttct gtcataatgt tcttttgcca taatttacaa acctcctaaa taatatagat
    79381 cactgaaaaa tttcagctct caccttttta ttatagtctt tttttgacta aatcaaaaag
    79441 aatacgtccg acttactttt tgtaagattc gaactaagct tattatacgt caatattggt
    79501 caaagtcaac taagagcact cgtctgtctt atacgcgaaa aaagtacctt ttataaataa
    79561 aaaaatgtgt tttttgagaa aaacttcaag atagtcctta actttctttt gaagcaataa
    79621 gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa caactttcaa tagacgaaat
    79681 cttctatttc gttaataata aggaaagagg tgaagtacat gaaaaaatta attggcgtac
    79741 ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc ttatcattat ttttacggcg
    79801 gcgaagctta ttatacaaaa atcacgacga atggcgaaat gtcagctacc caagcagata
    79861 acggagagaa attcacgact tatacctaca agcaagcggc ttataataaa gatggtgaaa
    79921 aaaaacaggt gacgcttcgc gaagaaagag aacatccatt aaagatgaat gcgtatctta
    79981 aactcaaagt caatcctcgt aaaggtgtat taagctggga agaaataaag gcttctgaag
    80041 tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat ggtcttaaaa ttttattaga
    80101 taaaa
//
LOCUS       DASJUF010000002        77976 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJUF010000002 DASJUF010000000
VERSION     DASJUF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.002"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(122..2308)
                     /locus_tag="U4B62_000076"
     CDS             complement(122..2308)
                     /locus_tag="U4B62_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN2140267.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2526..3209
                     /locus_tag="U4B62_000077"
     CDS             2526..3209
                     /locus_tag="U4B62_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN2140268.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3290..4009)
                     /locus_tag="U4B62_000078"
     CDS             complement(3290..4009)
                     /locus_tag="U4B62_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2140269.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4321..4527)
                     /locus_tag="U4B62_000079"
     CDS             complement(4321..4527)
                     /locus_tag="U4B62_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140270.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4772..5374
                     /locus_tag="U4B62_000080"
     CDS             4772..5374
                     /locus_tag="U4B62_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2140271.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5515..6795
                     /locus_tag="U4B62_000081"
     CDS             5515..6795
                     /locus_tag="U4B62_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2140272.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6808..7536
                     /locus_tag="U4B62_000082"
     CDS             6808..7536
                     /locus_tag="U4B62_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2140273.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7605..9128)
                     /locus_tag="U4B62_000083"
     CDS             complement(7605..9128)
                     /locus_tag="U4B62_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN2140274.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9380..10561
                     /locus_tag="U4B62_000084"
     CDS             9380..10561
                     /locus_tag="U4B62_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2140275.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10621..12249)
                     /gene="treC"
                     /locus_tag="U4B62_000085"
     CDS             complement(10621..12249)
                     /gene="treC"
                     /locus_tag="U4B62_000085"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN2140276.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12700..15411)
                     /locus_tag="U4B62_000086"
     CDS             complement(12700..15411)
                     /locus_tag="U4B62_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140277.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15421..16122)
                     /locus_tag="U4B62_000087"
     CDS             complement(15421..16122)
                     /locus_tag="U4B62_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2140278.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16308..16907
                     /locus_tag="U4B62_000088"
     CDS             16308..16907
                     /locus_tag="U4B62_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2140279.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            16988..18001
                     /locus_tag="U4B62_000089"
     CDS             16988..18001
                     /locus_tag="U4B62_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2140280.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18109..19026
                     /gene="rbsK"
                     /locus_tag="U4B62_000090"
     CDS             18109..19026
                     /gene="rbsK"
                     /locus_tag="U4B62_000090"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN2140281.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19054..19449
                     /gene="rbsD"
                     /locus_tag="U4B62_000091"
     CDS             19054..19449
                     /gene="rbsD"
                     /locus_tag="U4B62_000091"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN2140282.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19449..20822
                     /locus_tag="U4B62_000092"
     CDS             19449..20822
                     /locus_tag="U4B62_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2140283.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21179..23323
                     /locus_tag="U4B62_000093"
     CDS             21179..23323
                     /locus_tag="U4B62_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2140284.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23375..24415)
                     /locus_tag="U4B62_000094"
     CDS             complement(23375..24415)
                     /locus_tag="U4B62_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2140285.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24692..26494)
                     /locus_tag="U4B62_000095"
     CDS             complement(24692..26494)
                     /locus_tag="U4B62_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140286.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26508..27260)
                     /locus_tag="U4B62_000096"
     CDS             complement(26508..27260)
                     /locus_tag="U4B62_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2140287.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27269..27631)
                     /locus_tag="U4B62_000097"
     CDS             complement(27269..27631)
                     /locus_tag="U4B62_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2140288.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27807..28679)
                     /locus_tag="U4B62_000098"
     CDS             complement(27807..28679)
                     /locus_tag="U4B62_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2140289.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28730..29218)
                     /locus_tag="U4B62_000099"
     CDS             complement(28730..29218)
                     /locus_tag="U4B62_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN2140290.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29356..30147)
                     /locus_tag="U4B62_000100"
     CDS             complement(29356..30147)
                     /locus_tag="U4B62_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN2140291.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30202..30810)
                     /locus_tag="U4B62_000101"
     CDS             complement(30202..30810)
                     /locus_tag="U4B62_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN2140292.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30803..31120)
                     /locus_tag="U4B62_000102"
     CDS             complement(30803..31120)
                     /locus_tag="U4B62_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2140293.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31276..32004)
                     /locus_tag="U4B62_000103"
     CDS             complement(31276..32004)
                     /locus_tag="U4B62_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN2140294.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32163..33044
                     /locus_tag="U4B62_000104"
     CDS             32163..33044
                     /locus_tag="U4B62_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2140295.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33281..34324)
                     /locus_tag="U4B62_000105"
     CDS             complement(33281..34324)
                     /locus_tag="U4B62_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2140296.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34327..34995)
                     /locus_tag="U4B62_000106"
     CDS             complement(34327..34995)
                     /locus_tag="U4B62_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2140297.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35137..37473
                     /locus_tag="U4B62_000107"
     CDS             35137..37473
                     /locus_tag="U4B62_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2140298.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37588..37899)
                     /locus_tag="U4B62_000108"
     CDS             complement(37588..37899)
                     /locus_tag="U4B62_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140299.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38080..39555
                     /locus_tag="U4B62_000109"
     CDS             38080..39555
                     /locus_tag="U4B62_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN2140300.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39602..40186
                     /locus_tag="U4B62_000110"
     CDS             39602..40186
                     /locus_tag="U4B62_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN2140301.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40188..40607
                     /locus_tag="U4B62_000111"
     CDS             40188..40607
                     /locus_tag="U4B62_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN2140302.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40888..41172)
                     /locus_tag="U4B62_000112"
     CDS             complement(40888..41172)
                     /locus_tag="U4B62_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN2140303.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41524..42234)
                     /locus_tag="U4B62_000113"
     CDS             complement(41524..42234)
                     /locus_tag="U4B62_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN2140304.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42231..43703)
                     /locus_tag="U4B62_000114"
     CDS             complement(42231..43703)
                     /locus_tag="U4B62_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2140305.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43841..44314
                     /locus_tag="U4B62_000115"
     CDS             43841..44314
                     /locus_tag="U4B62_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2140306.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44467..45018)
                     /locus_tag="U4B62_000116"
     CDS             complement(44467..45018)
                     /locus_tag="U4B62_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2140307.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45123..45653
                     /locus_tag="U4B62_000117"
     CDS             45123..45653
                     /locus_tag="U4B62_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN2140308.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46136..46579)
                     /locus_tag="U4B62_000118"
     CDS             complement(46136..46579)
                     /locus_tag="U4B62_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2140309.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46595..47755)
                     /locus_tag="U4B62_000119"
     CDS             complement(46595..47755)
                     /locus_tag="U4B62_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2140310.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48262..49962
                     /locus_tag="U4B62_000120"
     CDS             48262..49962
                     /locus_tag="U4B62_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140311.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50064..50897)
                     /locus_tag="U4B62_000121"
     CDS             complement(50064..50897)
                     /locus_tag="U4B62_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN2140312.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51201..53366)
                     /locus_tag="U4B62_000122"
     CDS             complement(51201..53366)
                     /locus_tag="U4B62_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2140313.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53626..55920
                     /locus_tag="U4B62_000123"
     CDS             53626..55920
                     /locus_tag="U4B62_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2140314.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55913..56578
                     /gene="pgmB"
                     /locus_tag="U4B62_000124"
     CDS             55913..56578
                     /gene="pgmB"
                     /locus_tag="U4B62_000124"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2140315.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56571..57578
                     /locus_tag="U4B62_000125"
     CDS             56571..57578
                     /locus_tag="U4B62_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2140316.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57600..58619
                     /locus_tag="U4B62_000126"
     CDS             57600..58619
                     /locus_tag="U4B62_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2140317.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58900..60267)
                     /gene="nhaC"
                     /locus_tag="U4B62_000127"
     CDS             complement(58900..60267)
                     /gene="nhaC"
                     /locus_tag="U4B62_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2140318.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60376..61797)
                     /locus_tag="U4B62_000128"
     CDS             complement(60376..61797)
                     /locus_tag="U4B62_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2140319.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62040..63917)
                     /gene="tdc"
                     /locus_tag="U4B62_000129"
     CDS             complement(62040..63917)
                     /gene="tdc"
                     /locus_tag="U4B62_000129"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2140320.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64208..65464)
                     /locus_tag="U4B62_000130"
     CDS             complement(64208..65464)
                     /locus_tag="U4B62_000130"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN2140321.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65576..65781)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66044..67345
                     /locus_tag="U4B62_000131"
     CDS             66044..67345
                     /locus_tag="U4B62_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2140322.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67414..68013)
                     /locus_tag="U4B62_000132"
     CDS             complement(67414..68013)
                     /locus_tag="U4B62_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2140323.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68267..69148
                     /locus_tag="U4B62_000133"
     CDS             68267..69148
                     /locus_tag="U4B62_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2140324.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69161..70771
                     /locus_tag="U4B62_000134"
     CDS             69161..70771
                     /locus_tag="U4B62_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN2140325.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70848..71507)
                     /locus_tag="U4B62_000135"
     CDS             complement(70848..71507)
                     /locus_tag="U4B62_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2140326.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71790..72455)
                     /locus_tag="U4B62_000136"
     CDS             complement(71790..72455)
                     /locus_tag="U4B62_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN2140327.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72602..73207)
                     /gene="dhaL"
                     /locus_tag="U4B62_000137"
     CDS             complement(72602..73207)
                     /gene="dhaL"
                     /locus_tag="U4B62_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN2140328.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73235..74245)
                     /gene="dhaK"
                     /locus_tag="U4B62_000138"
     CDS             complement(73235..74245)
                     /gene="dhaK"
                     /locus_tag="U4B62_000138"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN2140329.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(74220..74666)
                     /gene="dhaM"
                     /locus_tag="U4B62_000139"
     CDS             complement(74220..74666)
                     /gene="dhaM"
                     /locus_tag="U4B62_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN2140330.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74769..75089
                     /locus_tag="U4B62_000140"
     CDS             74769..75089
                     /locus_tag="U4B62_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN2140331.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75201..76070)
                     /locus_tag="U4B62_000141"
     CDS             complement(75201..76070)
                     /locus_tag="U4B62_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2140332.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(76166..77470)
                     /locus_tag="U4B62_000142"
     CDS             complement(76166..77470)
                     /locus_tag="U4B62_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2140333.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77467..77976)
                     /locus_tag="U4B62_000143"
     CDS             complement(77467..77976)
                     /locus_tag="U4B62_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140334.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
BASE COUNT        23113 a        15856 c        13954 g        25053 t
ORIGIN      
        1 tgtgttccag aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca
       61 attttcgtac attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct
      121 atcactttcc ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt
      181 ttcttgaatt tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg
      241 caacataaaa tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat
      301 taaagtcgat tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac
      361 caatcaaaaa gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata
      421 gtgcatcaag ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg
      481 cttgggtttc ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt
      541 ctcgtatcca ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat
      601 agctttgaac aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa
      661 actctcggat ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg
      721 caacggctat attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt
      781 agcagtctgc catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat
      841 tcagtgctgt ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa
      901 agataccttc atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag
      961 attggacaag ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt
     1021 tcccattttt ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag
     1081 tatcagaaaa caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct
     1141 ttggtatccg atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg
     1201 cccgagaacg ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt
     1261 tcttgatgta tgctggctct gatcctctga atccatagat actctgatca tcatctccaa
     1321 caacaatcaa cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg
     1381 gattagtatc ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct
     1441 ggatctcctc attctccaaa gcgcttttca gattttggag tacatcattg aactctagca
     1501 attgctggct tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata
     1561 tcggctgtcg tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt
     1621 cttctactga aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc
     1681 catattgatc aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca
     1741 ctttttgtgc agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa
     1801 attttcgaca aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga
     1861 cagcggtttt tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc
     1921 ttgtaaaagt gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa
     1981 cagcttttgc acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat
     2041 gtttcttcat atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac
     2101 gtttctccat cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt
     2161 cctcaataag tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt
     2221 gtagccgaaa gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt
     2281 gtaatcggag aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg
     2341 aatttgttta tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa
     2401 cagacctttc taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc
     2461 ctttttttca atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga
     2521 aaaaaatgca gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg
     2581 gaggagccat ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg
     2641 gcaatatcat tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa
     2701 aagtattgtc tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt
     2761 attttagaaa aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga
     2821 tttttttctt tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga
     2881 ttgcttttac tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca
     2941 ctaatttatt tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta
     3001 tttctaaaga aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct
     3061 ttatcgcagg agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt
     3121 tgatcgtttc cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat
     3181 tttatcatac gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg
     3241 ctgctcacag agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg
     3301 taaagattcc ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct
     3361 cttctgtcat tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg
     3421 gatcggctac gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact
     3481 gttcatacca ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat
     3541 cttgttctag ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa
     3601 aggatacacg aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact
     3661 ccttcagcat cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc
     3721 ctaaatctgc attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt
     3781 tgtcatcatg gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct
     3841 gtaataaaaa agtatacttt tctggattag aataaaatag cggcaatact tcattatctt
     3901 ccacgttctc ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc
     3961 ttttccctgc accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa
     4021 taaaatctgt tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg
     4081 ctccttaaaa gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag
     4141 gctgtgacaa aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca
     4201 cctcggtcac aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg
     4261 taactcagag ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt
     4321 ttattcttct attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt
     4381 atcattgtct tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt
     4441 ttctattttt ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc
     4501 gacttcaaac aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt
     4561 aattttttaa cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta
     4621 tcaaaactgt cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca
     4681 tgacggtttt gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta
     4741 gcataaaatt tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt
     4801 agaagcctta ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt
     4861 tgtcttagaa gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct
     4921 ggtgacaggc tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag
     4981 atatgcttat ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta
     5041 tttatggggc gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat
     5101 ggtctttttg ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc
     5161 aatcaatccc attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt
     5221 tctaattttt tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct
     5281 acttgggatt gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt
     5341 tggaggaatc tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt
     5401 ttgtatctgg ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa
     5461 aataatacgc caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt
     5521 ttatggtcca tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat
     5581 cacatccgat acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga
     5641 tgggtctatt cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt
     5701 acttttttct tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta
     5761 ttgacgtcgc ttttaggaga tcacttaaca agcatcggcg atttttttgt tttgaaatct
     5821 tcggttattg aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta
     5881 tctgctttat tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac
     5941 cgccgtatga tcggtacgat cgggacattg atattattta catactatat cattattttt
     6001 tatacccgtt tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt
     6061 ctatatctgt gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa
     6121 ttagctgatc agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta
     6181 gaaaaaaact atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct
     6241 cttcacagct atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg
     6301 atattaccta cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat
     6361 atgaaagttc ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa
     6421 gggttgatcg tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta
     6481 aaccttgtac gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca
     6541 aagaagaaaa aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg
     6601 atcaatacaa caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca
     6661 aaaacaaatc caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc
     6721 aatccttatc ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat
     6781 atacaaataa aataaaggag taatgatatg attcccattt atatttgtga agacgatcca
     6841 aaacaattag aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac
     6901 agttatgtcc atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga
     6961 acttcttctt ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt
     7021 gtagcgatcg gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact
     7081 tcccatacgg aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc
     7141 gtcaaagaag atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc
     7201 gaagattttc aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag
     7261 atcaaatttt taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat
     7321 aaactggaag tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc
     7381 gagaaaatgc atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat
     7441 gtgcggtcga ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca
     7501 gcctctatcc gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga
     7561 ctaaatgacc agccgatcca aacccttcgg ttcttttttg cctatcagtg caaaagcatt
     7621 tcctgtgcaa tttcacgtcc atccattgta tctggataaa atgttggcca attctccatt
     7681 tctttcagca atacttcttg actttcattt cctgcatata aatgaaaatg ttcggagtca
     7741 gccggtgcaa tcgtgtgatc actgaattga atatatctaa aagcgccact ttccttatct
     7801 tctgcttcga atagatacct tacgccacgg tttcctgcct cgtaggtcaa gatttctttc
     7861 cctacataac gataatttga acgtttcgtt gttccattct taaacgtaaa tgccatctct
     7921 ttctcactga tcttgattga agcaatatct gtttgatagc ccttcgtata atattcttta
     7981 tattcttgtg ctgtcttatc ctgattcaat ttttctttat attcaaaaac aggatcaaaa
     8041 gtcccatctt ctacatacgt atctactgtt tgccaagttc ctgcccaatc tgaaagagag
     8101 cgatcttgta catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc ttttttgggg
     8161 gtcaaagtat cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc ttcgaggttt
     8221 gcttccatga tagaaagata gttttctcca ttttctattt gttcattcgt caatccttcc
     8281 aaaggattca atacttctaa agatacacct gcttcgtttg ctaacgttct ggcaatacta
     8341 tctttggcgt tttcttcaaa ataaatatag ttgattccat gttctttaac aaagtgtttc
     8401 agttcagcaa ttctcgccgc agacggttct tcactagaag aaagaccagc aatcgatact
     8461 tgattcaatc catattctaa cgctagatag ttgaatgcag catgttgcgt gacaaaagat
     8521 ctttgttttg cttgcttaaa ggtttcttga taaagctgat ggagtgcttg aagtttctcg
     8581 gtataggctg ctgcattttt tgtccatact tcctgttttt ctgggtaagc ttcaatcagc
     8641 tgatcacgta tgtttgctac ctgcttgatt gctaaacttg gagctagcca tacatgtggg
     8701 tctagctcat gtgtatggcc ttcttccccg tgttcatggt catgatcatg tccttcttca
     8761 cttcccggaa gtaaaagcat accttctgtc gcttttatcg tatggacatt gccttcttgt
     8821 aatgtttttt ggattgcggg gacccatgtt tccatgtttt catcattata aacaaagata
     8881 tctgtatctt ggatctttgc catgtcttta gcagaaggtt cataatcgtg ggaatcagtc
     8941 cctgctggaa tcaataactc tacttctcct tcatccccga cgattgcttt tgtaaaatca
     9001 tacatgggat aaaaggtcgt catcacgctc aatttttctt tactgtcctc ggctttccca
     9061 cttgccccgc aagcagttaa catacctgct aaaaccagca tggccaatat acctatcaat
     9121 cgtttcattt atgacactcc tcttctcatc ttctttcatt tgaatgttcc gattcgcttt
     9181 tcgcaaaccg taatagttac gttttagcaa caggatttaa tttatcatat ggttaaaaga
     9241 actgtcaagc gttttcttat tttaattgat gagaaagttt atatgaatga ttgacgatat
     9301 ttttgcaatc aacctctatc attttccttt tatacccgct tttgtctata atgaatttat
     9361 agataaagga ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt
     9421 tttaggagga aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat
     9481 ttattttttc catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat
     9541 cttgccccca ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg
     9601 tttgagcaag cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt
     9661 tttattgggg cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt
     9721 gatcagtagt ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga
     9781 gaaaacacct tttgccgatt catttaatca atttttcgct tctattgacg acgtgacgtc
     9841 tgatatagct ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat
     9901 tttttcaact gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt
     9961 ccttttgaga gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt
    10021 tcgtacagat tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa
    10081 ggggcaaatc attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat
    10141 cggattagaa tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata
    10201 tattgggccg tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt
    10261 tatggctgtc aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt
    10321 gattcctcga gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt
    10381 acttgtcatg ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac
    10441 gttagtcaaa ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt
    10501 ttatggcgat aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata
    10561 aaacacaaaa cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt
    10621 ttaatttttc agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac
    10681 agtatcggaa taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat
    10741 ccttgtatct ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg
    10801 aagaaagcca taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac
    10861 gattggatat gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc
    10921 agaagatagt tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg
    10981 tttccctgta gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt
    11041 atcgcgagat tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag
    11101 gagtaactta taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc
    11161 tgggtcgatc atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg
    11221 gatcatggtt gctagcatct ctgctccttg ttttcgatac tgatcgatag ctacaaatcg
    11281 attgagcgca cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc
    11341 actcatcttt tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg
    11401 agtagaccat ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat
    11461 tgataattct tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct
    11521 catttcaccg actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt
    11581 tagatacgta tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt
    11641 atttttcaat tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac
    11701 ccctttttcc atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg
    11761 ccagttcaag tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc
    11821 aaatggcgac caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa
    11881 aatgaagaag tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc
    11941 tgtcgaaacg tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc
    12001 ggctattagc tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc
    12061 agagatatca taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat
    12121 gtcgatgcct agatcattca gatacgggag tttttctttg attcctttta aatctccaat
    12181 tccatcacca ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt
    12241 aaatgtcatg gatatttacc tccattcaac tgatttatta cctaccacaa cttatatata
    12301 caagttatca tttattatta taactgaacc gaaagcgttt gtaaaattta tctttataca
    12361 ttctaagagg tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc
    12421 ttgtttaaaa tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc
    12481 tacggcaatc agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt
    12541 tttgctcgat gcaggacggc ttttttacaa cctctgattc atggtgttca atcagctgtc
    12601 cttcggtatt agctcaaaaa acggcaaaat atgaaaaact attttgccgt ttgtttctct
    12661 aatactcagg tcaaaccgct gatttcacaa cctctgtttt tattcattcg atttcaacgc
    12721 atcaatcata tcgatatgct ttaatttgaa atgggtaaca atcatcacaa taaccgtaaa
    12781 tacgatagtt aatcctgctg ataagacata agcccaccat gtgatcacta atggaaagat
    12841 gacattctcc atagacgctt gttgcaagat gaagtcagtc aatatatatc ctacaccaaa
    12901 tcctccgatg attcctaaaa gagtaaagat gatattttct cgaacaatgt acatcgtgac
    12961 ttctttatca aaaaatccaa ggacttttat tgtagataat tcgcgtacac gctcggaaat
    13021 attgatatta gttagattat acaagacgat aaaagccagc agtcctgata atacgacaaa
    13081 gattaagaca accgagtcta agttcgccat tgattcttct tgcgtttcga tttgtttgga
    13141 catgaaagac gtatttgtca cagcgtctgt ggcaagcagt ttctcggaaa gcatttcttc
    13201 ttcttttgtt gtcatcttat ctgtacgcag caaaaatgca ttatccatca tcgattgatc
    13261 agtaactgaa gcataatatt caggggacat ataaataaag ctacctagat agtttttcac
    13321 gatttctttt acttcgactt tccattcttt ttgatcttca tcataaagcg aaatatgatc
    13381 tcctgtagat aagtcaaata gttctgctac tttttgcgta atagcgattc ctgttttagg
    13441 caaggagcgt gggttctctt caagatcagt taaatggata aaacgatcca aatcagctgt
    13501 tttacttggt acgatcactg tcagactttg cgaagcatgt cctttttttc tcaattctac
    13561 ggtttgagta tgaatagcta ggatctcatt tactttttct tgcttctgca gcatatccgc
    13621 tgctttttga cgactatctt ccgtggatga atccaatgta acgattgctt gatagtcaat
    13681 gatcggtcca aattgtttgg ctgaaacaga acttagagaa tctttcaatc ccacgcctgc
    13741 aaccatcagc ccggcacatc cagcaattcc gataatcgcc ataagcattc ttgacttata
    13801 tcggaagaga tttcggtaac tgattttttg attaaagcta aggcgtgacc acagcggagt
    13861 gatgtattca agaaaaatgc gtttccctgg cttcggtgct ttcggttgaa gcaatgcagc
    13921 aggccgttct cttaaatctt tgaataaaac gatcattgca gcgccaaatg cggcaatgaa
    13981 gaatgcggca gctgcttgta aaatcggcgg ccagtcataa aaaataagcg cactcccaat
    14041 atcataccgc tcgttagaca gctcaaaaat gatccttggc aaaagattcg ttcctaatat
    14101 cgttcctaaa acaataccta tccctgaggc tagacttgca tagattgcat atttttttgc
    14161 gatttcaact ttcgtataac ctaacgcctt taatgtcccg atttccctgc ggttttcttc
    14221 taccatacgt gtcattgtcg taaaggtgat caatgcggca ataaagaaaa agaaaacagg
    14281 aaaaacattg gcaatcgcag cgatacgatc agacagatca ccaaattctt gaaaaccgac
    14341 attttctgaa cgttcattgt ataggtatga tggtttagtt aaattttcaa ttttttctgc
    14401 atttcgattc aattcttcct ctgcagaggc cagttcacgt tctttctcaa ctaattgcct
    14461 ttcttgttca gcaagttgag cttcttgttg agataatgtt tgaacagcga ttgttcgttg
    14521 cgcttcagga agctgctgga tagttgctga ctgttgcgct aactgttttt tagcatcttc
    14581 taaccgtgtt cttgcttgcg ttgtttgtgc tttgccatcc gctatttttt gctgtgccgg
    14641 ttctaattct tcgttggccg attttttcag ctcttctaag cgttcttcgg gtcgatcttg
    14701 taaatacttt tctgcctttt cttgattttg ctccatttta tctttgtaag ctttgctgta
    14761 cgtattcaaa ttctttacgt ttgtaaaatc aagatataag acagaataaa tatctgtttt
    14821 aaaaacggat tcaggtaaat agacaaaata atcgacgcta ccatttccga cgttcgcgta
    14881 tcctctctcc aaattgttga tgaacaatgg agaacggaca aagcccgtga tggtatactc
    14941 tttttctttc aattcatctg aatcaatctg ataaatatca ccgattttat acccttcatc
    15001 ttccgctttt tcatccaata caagctggtt tggtttttcc gggagttttc catcagttaa
    15061 ttgtagcttg ttctgcttgt tatttttgtt ataagaaaat atttgaatca cttcgttttt
    15121 gccagtattg gaataataga tctgttttcc tgtttctact tttgcatctg ggatcttttc
    15181 tgcttgagca atatcttttt gtgtcaagcc accagtagaa acgatttgca aatcagaaag
    15241 tcctgctgta ccaacatagt gatccatcgt tttttgcatg acaggtccag cagttttgac
    15301 acctacaaat aaaagtgttc ctagcatgac aataaggatg attgcaatga atcgtccttt
    15361 agattgccga atttcgcgaa aacctgcttt aagatatgtt cttgttttca taaaatcccc
    15421 ctaccataca atctcatcaa tggatgaagg atgatcattt aattcaacag accttacttt
    15481 agcatcatta atgtgaatga ctctatcagc gatgggcgct aaagcagaat tatgtgtaat
    15541 gatcaaaacg gtattgccgt gttgtctgct agcgttttgc aataatttca atacttgctt
    15601 gcccgtttca aaatccagcg cgcctgtcgg ttcatcacat aaaagaagtt tgggattttt
    15661 agctagtgca cgagcaattg aaaccctctg ctgttcgcct cctgataatt gggacggaaa
    15721 gttattcaat cgatgagcta ggcccacttg cctcaatacc tctacaggat caagcgcatc
    15781 aggagaaact tccgttgcca attccacatt ttcttttgct gttagattag gaacgagatt
    15841 gtagaattgg aaaacgaacc ctacatctgt tcgtcggtaa gcagtaagct gtttatctga
    15901 aaattgcgca atatctgtgt catcaataat gatctgacct tcatcaggtg tatccatccc
    15961 acctaaaata ttaagaatcg ttgactttcc tgctccactt ggaccaagta taatcactaa
    16021 ttcgcctttt tcaattgaaa aagaaatatc gtcatttgct gtaatggtcg tttctcccat
    16081 tttgtatcgt ttgtattcat tttttacttc gacatacttc actctgattc ctccattttc
    16141 acgatatttc tcgctatatc tatgcatcga atgcgttttc tcacacttat ttattttacc
    16201 actgaatttc tctagactaa agaaaggaat cctttaattt agttgacaaa taaacgattt
    16261 atgatttact tacataaaaa acattgttat ttgaaagaag gaacatcatg aatgatttaa
    16321 aggaaaatat gatttttgat ttatcaaaaa ctgcttttgt cgcgatcgac atgcaaaaag
    16381 agatcgtaaa taacggtaca ctctctccac acactgcagc atctattctg acaaacaatg
    16441 atttgttagt aagaacattg aaacgtaccg ctgccttgaa agttttagtg aacgtagaca
    16501 tctccacttt cccttatttg agtcaacaaa ctgatatggg tggaatgact gcttctgtac
    16561 ctccagaatt cactgattta cttttgaagg attctttgaa agatacagat aatatgttga
    16621 ctatcacaaa atacaatcca agtgcttttt ttggtacttc tcttgatctt caacttcgcc
    16681 ggcgtggaat cgaaactatc attttaagcg gtgtagcgac aacaaacggt gtatatgcta
    16741 ctgcattaga tgcctttcaa cacgggtacc atatcgtgct tgctgaagat gcttgcagtg
    16801 atcgtgacaa agaatcccat caattgttca taaaaaagat atttccgaaa actgcacgag
    16861 tacgctcaac gaaacaaata atcgaagcta tccaacaaag caaataagta ctggcacacg
    16921 tgattttcat ttacttcttt ttgatttcat gggaaaataa ccacaggatt tagataggag
    16981 tatgaacatg gtgaaaaaaa agatcacaat caaagaagtc gctaaacttt ccggcgtttc
    17041 aatcacaact gtctccttga tattaaatgg aaatacggag aaattcagtc cgaaaactgt
    17101 tcagaaagtt ttagctgcca aagaagaatt aggctatcag cctgactatt ttgctagaca
    17161 aatgattacc aaggaaacga aaacaattgg tgtactcgtt cctgatatca cgaatccatt
    17221 cttcagtaca ctgatgcgag gaattgagga tatcttgtat aagcagaatt tcgtcacgat
    17281 attatgtaat gctgattctg atcatcaaaa agaaatcgaa tatctagccg agctcacacg
    17341 tcgaggtgtg gatggattca ttatcgcgac ttctgctgtc tctacagatg caatcaacga
    17401 aaatttgaaa aaacaaggcc ggcccttcat tgttttggat cagaaaaaat ctgaaggatt
    17461 tagtgatgcc gtacgaacag acgattttcg cggtggttac ctagcgggca tgcacttgct
    17521 tagtctagga catcaaacga tcgcccttgt ctatcctgaa aatccaccgg aaaatgtcca
    17581 cgctcggatt gaggggttta aatctgctct tgatgtttac caaatccctc acgaccaact
    17641 gatcctctta cctacacagt tctcaaaaca aggtggctac cagatcacag cagaacttct
    17701 tgattctgct gcaactggtg tgtttgcttt aaatgatgaa ttagcttttg gattatatcg
    17761 cggattagag gaagctggaa aaagcatccc tgaagactac agcattattg gctatgataa
    17821 tatagatatg tgcgagtata tcaagccgaa actgacaacg atcgcacaac caatcttcga
    17881 attgggacaa acatctgcca agttattgtt agatcggatc caattcccag aaaaagaatg
    17941 ggaagaaaaa agattacctg tacgattaga aaaaagattt tcaactgcac ccttgaaata
    18001 gctttcaagg tgtgctatat ttttatcgaa ctattaaaac gttttactaa aacgttttaa
    18061 aaaataattt tcccatcaaa ataaatgaga tagttggagg tttgtaaaat gaacacggtt
    18121 acagtaattg gaagtattaa tatggacact acccttcgtt taaccaacat gcccaagcca
    18181 ggcgaaacga tgcatgcgca tgagattttt cacgctggcg gaggtaaagg tgccaaccaa
    18241 gcagtggcag ccaaacgttc aggcgcacgg acatctttta ttggaggcgt tggggctgac
    18301 agcgaaggac aacagttatt agatctactg acaaaagaaa acatcgatac atctgggata
    18361 gctgaaatcc aaggcgccac aaccggacaa gcaatgatta tggtagacgc agcaggagaa
    18421 aatagtatct tgatccatgc aggggcaaat aatgctttcc atgaacaaga agtcctaaaa
    18481 aacaagcaac tgattacaaa cagtgacttt attattgctc agtttgaaag tagcttagat
    18541 gcgacgatcc tagctttttc tattgcaaaa gacgcaggaa aaacaacgat cctgaatcct
    18601 gcaccagctc gtgaaacgat tcctactgaa ctacttgaaa agacagatat cattatacca
    18661 aatgaaacag aaacagaaat cattacagga atcagagtga cagatcataa cagtttggtc
    18721 gcagcagccg aaaaactcca tgaattaggt attggaacag taatcattac actaggcagt
    18781 gccggcgctt tctatcatac agaaaaagaa cacggtatcg tccctgcttt taaagtggat
    18841 gccgttgaca caacagccgc aggtgataca tttattggcg cattaagcag cacacttcag
    18901 ccagacctta gtaacttgaa agaagcgatc ctttatggaa atctagcctc ttctgttgct
    18961 gtgcagtctt atggtgcgca accttctatc ccttaccgcg aagctttaga acaatcagca
    19021 gtctaaaatt aacaatgaga ggaagaaaca aacatgaaaa aaggaaaagt aatcaattca
    19081 gatatttcac gagtaatcgc acaaatgggg cactttgata aattaagtat cggggatgca
    19141 ggcatgcctg tcccctttgg tactgaaaaa atagatttgg ctgtctcaaa cggtcttcca
    19201 agttttttat ctgttttaga aaacgtttta gaagaacttg aggtacaatc gattgaattg
    19261 gcggaagaaa taaagacaat gaacccggaa atattggaaa aaatcaaatt attgttacca
    19321 gatacaccga ttagctttat cccacatgaa gaaatgaaaa aagagttgaa taactgtcat
    19381 gcatttattc gtacaggcga aatgacacct tacgccaata ttatattagt gagtggagtg
    19441 gtcttctaat ggaaacgaca atgaatcctg aacagatggt actgtccgag cagattcata
    19501 tgctgattga acagttggga ggaaaagaca atattgattc tgtccttcca tgtgcgactc
    19561 ggcttcgtat caaattgaaa aaacagccag tagccaatgc agaaaaatta aaacagcaaa
    19621 tcccttccat taagtatttc attttactcg atagatatgc agaaataatc atggatcagg
    19681 gattggctcc tgcttacaag gaattgacgc gtcaaatcga tcattcagct ccgaaagaaa
    19741 agctaaatcc gatcgaaaaa ctatttggtt tgatatcgga aatctttacg ccgatcattc
    19801 cgctgtttgc cggttctggt atattaaaag gtctggttgt tttagcgact acgattggct
    19861 ggcttagtga aacaagcgga acttatcata ttttgtctgc tgcgtcgaat gccgtctttt
    19921 attttctgcc aatctttctt gctttttctg ctgccaaaac gtttaaagtg aatggatata
    19981 ttgcggctgt cattgctgca gcactgattg aaccgaatct gacctcttta ctttcagaca
    20041 gtggcaaagc agctactgat tttctgggga ttcctgtagt actgatgcag tatacgtcta
    20101 ctgtcatgcc ggcgatttta ggtattttct tttattcttt tgttgaaaaa ttcctcaaaa
    20161 aatatattcg cgaaaatatg caattagtat ttgtaccttt actttcatta gttatcacgg
    20221 ttcctttgac gttaatggta ttcgggcctg cgggtgttta ccttggcgaa ggtttagctg
    20281 ccgcaattac ttggatgatg gatattaacg gaccgatcag tggcgcaatc atcgctggtg
    20341 gttggaatat tttggtaata tttggtatcc aatgggctgt aaaccctgtc atgatcagta
    20401 atatctctgc ttatgggttc gaccgaatcg ttccgttgac aggagcagcc aattttggaa
    20461 tggctggtgc agctttaggt gtatttctgc gaagtaaacg ttcaaaaacc cgttcgatct
    20521 ccggttctgc ttttgcttcg attttacttg cgggagtgac cgaaccaact gtttacggaa
    20581 ttgcgatccc attgaaaaaa ccatttgttg ctgcttgcat tggtgcagct gctggtggag
    20641 ctgtaatggg atttgcccaa gtgaaagcga tcgcttttgt atttggtagt ctgaccacat
    20701 tgcctgcatt catttccggt acttttttct ggtacttagc aggactggct gttagtctag
    20761 tcgtagcaat gatcactact ctcgttagcg ggtttgatga agacctaatg tcttatgaat
    20821 agttaatgaa aaagactgaa gtatgagatg tttatcacat atttcagtct ttttcttttt
    20881 aggatcatta tttttataaa tttttgtgat actttccaaa ctttggtata atttacatat
    20941 gcataaccat ttttatcccc acttgtttat ccccaaacaa aggtggtcta tgcaataaaa
    21001 tgaacgtcaa agaaaaacga atgaatgctc gttgcttatt tacaaattgt tttatttaga
    21061 atacacagac agatttatga aaagaaaaag cttttcgaca cgattatctg ttccaaaaaa
    21121 tgtctaatga atgctgaata agacagaaaa tcaatgaaag gaaaaggact agtttattat
    21181 gaaaaataaa aaaacgatac gtttgctagc tgctacttgg ctgattgctc cgtctgtttt
    21241 gtcgaccaat accgtatttg ccgtagataa tcaaatagct gatcaaacag tcacaacaga
    21301 tacgactaca tccagctcag ctactagtga aacaacggat tcttttacag aagacccaac
    21361 tggaacaact aactcaacaa caggatcgac cgagccaact gaaattgatc cggctgatcc
    21421 atcagaaaat gaaaagccag ccgagacata ttcccttaca attgctcctt ctttgattgg
    21481 taagatccaa ctgactgctt cgacagcgga ttcccctgaa cagcaacctt tggatattac
    21541 ggaaagtgga acggtagaag gtttattagc ggaaacgaag gtaacttaca ctattacgcc
    21601 agctgaaggt gaacaaattg cttcagtcgc tgttaatggt gccaaagaag taaattatga
    21661 tgcaaagacc tttacagtag gtacgaacaa tttatctatt actgcagaat ttaaagctgg
    21721 accggatact tcaacagaga aaccaactga tcctagtgaa gaagtcccta gtacaccaga
    21781 tccggaaacg ccaaacaaac cagatgtggg taataacaac aataataatg gttcaaataa
    21841 ttcaaacaat aataacaata attcgaacag cgggaattct tcgaatcaaa caaacccatc
    21901 tggatcgaat aacaatggaa atactcagag agaaccaaac catccaaaca atggttcatc
    21961 gaatggcgga acgaacaatg cttcatctcg tccaaataca gcaaatcaaa gcggtataga
    22021 gaatccatct tctgcttctt ctgattttgt tgtaaaatca ccaattgatg ctgtattgcc
    22081 tacaaataca acgaatgtcc aacaagcaat cgttcgagaa gcattcaagc atcttggaaa
    22141 accatatgta tggggagcaa aaggtccaaa tacttttgac tgttctggtt tgacttatta
    22201 cgtatatatg aaagcaaccg gtcattatat cggtggctgg acaggcgaac agcaatatgc
    22261 aggtacgcag attccagtaa gtcaggccca acctggcgac ctcgtcttct ggggaccttc
    22321 ttccggtgtt actcaccatg taggtatcta tattgggaac ggacaattta tccatgcgcc
    22381 tcaaccagga gataaagttc gagtaacttc tatttcagat ttcacgcctg attttgctgt
    22441 ccgagtaaac ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg gctcatctat
    22501 tttagatgga ttagatggct cgttccattt cagtcaaaac caaacaactg atcagttctt
    22561 gaaaaagatt gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct acgcatcagt
    22621 catgatggct caagctatcc ttgaaagtgg atctggaaac agcttattat caagcgaacc
    22681 aaatcataac ctttttggta tcaaaggatc atataaagga agcagcgtga ccttcaatac
    22741 acttgaacaa gatagttccg gacaaagcta ccaaatccgc gcacaattcc gtaaatatcc
    22801 ttcttacaag gaaagtttgg aagactatgc cgacttgatc aaaaatggac tgactggcaa
    22861 tcctgatttc tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag aagcaaccaa
    22921 atatttggaa ggacgttatg ctacagatcg ccaatattca caaaaattga acgcgatcat
    22981 cgaagcgtac gacctgacaa aatatgatga accgaaaaaa gaagcagcaa caactgaaga
    23041 agcaaacgct gaaatcctca atatgtctaa gcgattcgat gtgccaatca aatggacaac
    23101 agatggcgtg ccaatcacag gattagaatt ttcaacgata aaacgcgtca acaacgcttc
    23161 ttccatcttc cgtgtcgctt caatctggga tttgtggaac aattttacgg caagacagat
    23221 accagaaaca actactcgta cagttaaagc tgcaactgtt cctgtactaa ccttgttaag
    23281 tttagacaaa tcattctggc ttgatctgcc tttaaagaaa tagaataaat taaaaagggg
    23341 atgtgacact gtcacactcc ctttttaatt tattctattt ctttatattt attttgtatt
    23401 ccgttacaaa ctcgcgtact tggttaggtt ccagagtgac atctccaaaa ctttcaagtc
    23461 gctccgctcc tggcagttct tgagtctcaa atgtgatacc gccatgattt actaatttat
    23521 tcccgcgcat ttccggtcca tcttctccaa aatttgctgt aaaaatcacg attcctgaag
    23581 catctgtttt tacttggatc gatacttttt catctgggct tgtcaattcc gcagcattta
    23641 gtaaaccaga ttgttttttc aacaaaaatg gatgatcgat gccgcccact atcattgatt
    23701 ggcgaaaatc actggcaaag acctcaccta attttttagg acgttggaag tcaaatgctg
    23761 tgccacttac tgaaactact tctcctgtag gcgttgtatc tttattgagc gtagcaaata
    23821 atcggctatt caaccaaagt atatgttcgt cgattgactg tgctggatca ccggttaaat
    23881 tgaaatagac atgatttgta ggattgaata ctgttttgcg gtcactgatc ccttggaccg
    23941 tcactcgcca tgtatcatcg ttcgtcagcg tataccgtac ttctacatct aaagttccag
    24001 gaaatccgtt ttccatatct gggctagtct gatagaaaat gaccgatgct tcgttttcac
    24061 cattgatgac tgtataatgc cactgcttgg cttcaaagct aggtgcacca ccatgtaaac
    24121 aatgaccagt gtccgggtct gtttttactt cgaacgtttc atcatctaat ttgaatgtgc
    24181 cattcgtaat ccgtccagcc atacgtccga ttgaagcacc gatataaggg tctttttcta
    24241 gatattcttt tgcttcatca aaacctaaga tcaactctcg tttttcatta tcgattattg
    24301 tttgaaaatg aacaacccgt gccccaagat cactgacaga caaagatgtc ccattttgat
    24361 tcgtgatcgt aatcaagtga tagttttcgc caaaattttg ttcattgatt tccatgttcc
    24421 ttgcaccctc ttttctttca tttgcactga ttccagtata catggaaacc gttttcttaa
    24481 cagcttattc cgtgagtaac gagaaaaaat ttactatatt ctaataaaag accgtgaaaa
    24541 aaaggttgtg acaaaaatat tgtcacaacc tctttttccg aacaaacggt gttcaaaagc
    24601 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg aggagctatt tttgcttggc
    24661 tgttcttatt acttgatgca ggacagcttt ttcacaatct ctttttcaat acgattccag
    24721 tatataacca tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat atcgtaaacg
    24781 gaacccaaat atctttgtat ggattttgca tgaatagacc aaacatttgt aatccgaata
    24841 agacatggac tacacctaag attcctggtg ctaagaataa cacgcctact tctctacgga
    24901 tcgattgttt aagcaacccg cgtcgtgttc ctatcttttc aagcatttca taacgagcaa
    24961 tgtcactctt gcttcctgac aagatcttga acatcaaaca gctagcaagc atggtcaaga
    25021 aagctaaacc taggaagaat cccataaatt caaaaccaga gaatagacca ttatacactt
    25081 catacatttc atatttctgg ttgctgtact ctactttttg caaagatgga ttgttcattt
    25141 tattttgagc aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct tttacctgaa
    25201 ctagctgtaa agtggattgt ggcaagttca gttgatcaaa ttctgattga ctgactacct
    25261 tgatttcttt ttcttgctgt tccggcaatt catagcttcg aagttcctct tgtacagtca
    25321 gttcattcat ttttttagca ggaacttttt ctttcgtgat cttcggcggt gttccatctt
    25381 catgttttat catgatcaat ggttgagccg caaattcact tgcattatag taaatcgttc
    25441 ccgcatcttc tttttgagta taagaagcag aaagtgttgg tgaaagttct ttgatttttt
    25501 gctgatcttc tgcccgagga ttattcatca ctagatcata tccagaattg ttttctgtca
    25561 agtctgcaat ttcattttta aaacctaatc ctaccgtcaa agcgcccagt gccaaggcaa
    25621 acagcatagc gaccattgag agcatttgtg tatattcacg aatacgaaag tttaactgtg
    25681 acaatgtgaa attgttcaat tttttcaatg aaatctgttc tgatttcttt agtaatgata
    25741 aaaagaaaat gatcacagaa tggaaaatga aataagttcc taatacgatc gtaactaagg
    25801 ctacccctat accaaagact tgataggtcc ccacgtgttt catcatatag taaccgcagc
    25861 ctagaaaagc aattcccagt actacttcaa ggaaaaacaa gaatttatgc tgcttgattc
    25921 ttgttggtgt ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca atcgaaaaag
    25981 cattgacaac agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg atcaacaacc
    26041 cgtgaatact aaaaggtgta aaatgcgaaa tattgagatc taactgatcc actaataatc
    26101 ggtggacgat acttgtcaat cctacaccga cagctgaacc cactaaagta gctgcgatcc
    26161 ctactaagaa tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt gctcccagca
    26221 tcataaacat tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt gcatataaaa
    26281 tataaacgaa tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg ataactgtag
    26341 ccgcaacgat cgtattactt ttcaaaaaag cttcgttgct tgccattgat tcaaacatgt
    26401 aaaaaatagc agatgccata actaatcctg agaataacac caagtagtct cttaaacggc
    26461 tcttgatccc agtaagtgat aatttccaaa gcatagtcaa tctcccccta ctgttccaag
    26521 tttccaagaa tagatagtat ttctcgatag aaggaatcac ggctttgatc tccacgtttc
    26581 acttcttgat ggataccgcc atccttgatg aataaaatac gttggcaata acttgcagaa
    26641 aacggatcat gcgtcacaag aagaatcgat acatgatctt ttgtattcaa ttcgtccatc
    26701 gtatctaaaa gatcccgggc acttttggaa tccagcgcac ctgtaggctc atcccctaat
    26761 agaatcgttg gttgtgtgat caatgcacgt gcggcggcga cccgttgttt ttgtccaccg
    26821 gaaatctcag aaggatagct ttccaaaata tgagagatgg ataaacgttc tgcaattttt
    26881 tggacacgtt gcttgatttc ttttggtctg acgccttgta aagaaagagg aacagcgata
    26941 ttttcttgtg ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa gccaatctca
    27001 ttcgcacgaa aatctgctag ttgatttcct tttaatgtgg tcacatcttt ttgattgatt
    27061 cggatatgcc catctgttgg tttatctaat gtggataaaa tattcaaaag tgtcgactta
    27121 ccagaaccag aagcgcccat gatcccaata aattcgcctt tttctacatc gaagctgatg
    27181 ccgcttaatg cttgcgtctt tttctcattg ttttttccgt atacttttgt tacattctgt
    27241 acttcgacta ttttgttcat ttctttccct accctttaat tagtttttcc agtgctgttt
    27301 tcggcacgtc ttttttttcg acttcttcat acgaagtgac acctttattt ttgttccagg
    27361 tgatttttaa atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg aagtccagtt
    27421 ctttttcctt accctctttc gtatagccag gcaggatata tctatagttg actgtttctt
    27481 gtccagagtc tgattttgaa actgtttttg taccatctgt cgtaatttgt acatagtatt
    27541 cttctccacc cataactacg taatctgcga ttttcacccc tgcaagtacc acaacgatga
    27601 aaacaagtag accgatcaga agttttttca ctttttcccc ttctctctta ctttatatac
    27661 tgattataga aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct tctttcaatt
    27721 ttgtcatttt ctttaaaaca actaaaaaaa gagttcaaga caataatccg tcctaaactc
    27781 cttcttccta tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc tgtattgata
    27841 acaggttgcg ttcctttgtc tgtaatgatt gagacaagta aattattgat caattggact
    27901 ttccgatcat cggtgaaatt gatttcttgt ccatcttcga tttgctctaa ggccatttga
    27961 gtgattgtga cagctccttc aacaatggtt tgtcgagcag acaaaatggc ttttgcttgt
    28021 tgacgttgga gcatggcact agcgatttct gttgcatagg ctaaatggtt caagcgtgtt
    28081 tcgattactt cgacacctgc tactgctaaa cgctcttgca gctctttttg caactcgtca
    28141 gacactgccg tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt atcatacggg
    28201 tattggctag cgatatgccg aatggctgtc tcactttgga tttctacaaa atcttgataa
    28261 tacgctacat cgaataatgc ctttgctgta tcaacgactt taaagactac tactgctgag
    28321 atttcgatgg ggtttccatc caaatcattg acttttaaaa cagaactatt gaaattccga
    28381 actttcaacg aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa gccattttct
    28441 cgaatcgtac ctaaatattg tccaaaaaac aaaattactt ttgcttgatt aggactgact
    28501 acagttgccg aacttaacaa caataacgtg atcaaccaaa gaaaaatact taaaaaaagt
    28561 gcccaaactt tcgcttgcca catacctaaa tagaagaaaa acaatccaac taaagctaat
    28621 acgaccaatc cgacaatccc tgcataacca ttgatataaa atgctctttt ttctctcatg
    28681 ctacttcctg cgctccttta tccttcaata aatatctaat gttacttttt taattttttt
    28741 ttcgaatacg cgccattaca ccttctagtt ctaactcttt gtcttcagtg atgtaccaac
    28801 cgcttgcctg tgtccctccg gctgcctgcg gtaaataatc caattttgtt cgaacccagt
    28861 gcttttcttc tgtaaaatag tcttctacca agacctctag atccatccct gctgaaagag
    28921 gcaaatcttc ccgaacagat ttaaagataa atcgttcctt cttcccatca tacttcaata
    28981 ttcctgtatc gataatcggt aaagaataat aatccagctc tttcttcacc gtcaataaat
    29041 gtttgaaaac ttcatggtac acttgctcta attggcgacc ttctgaattg ttcgggtaat
    29101 taaccatcac tttttctttg tctggatcat agaaggtttg gcttagttga gtgatataag
    29161 tatgtaatct ttccatttct gtttttaact tttctctttg accttcttct accttcatat
    29221 actctttcac cctgttttct ttttagatat tttctttctt attttactat agttatgtgc
    29281 gttaagcgat gaaaaacagc aaagattgga aggatctttg ctgttttttc ttaaaatatc
    29341 cagttgagta caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa tattgatcga
    29401 tgtgtcttta gcagtgatct ttagcttttt ctgtccttgt tctaacaaag tatttgttat
    29461 tggctggtag tctttttcaa tataagattc accagacgtt tggatagtat agccactgtt
    29521 ttccatctca tgcaaagata aattgccacc gtttaggatg acttttcctt ctttgctgat
    29581 caaactgtct tctagatgga agttgctgtt ttcacctgtg acgatcaagt tatccacatt
    29641 ggcctcatct aatcgaagtg agcctaattc atctttcgca gttattgttc ctcctgtaaa
    29701 ttcattgaac gatacccagc catcatgtgt cttagtattc acttttttga ttgatacttc
    29761 gttgatatca attgcactat tattcccttc gattgtcaat tcagataatt catgatctgc
    29821 agggacaaca atagtgattt gtgcacttgt gtcttctaaa ctaatgattg cacctgttga
    29881 ttttttgact gcattcacag tcaacacgcc atttttctcc tttatttctg gcttttctgt
    29941 cttatcataa gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa ctgtttgatt
    30001 ttctgcatgg atgacgattt tgtttgcatc cttgattttt tttgtctcag taaccatatc
    30061 gatcaaccga ggaccattat cccaagtcag actcttttga gcgcctaatg tataagttgc
    30121 acctgctagg aggccaccag taagcatcag accaataccg gtaatcaaaa aatgtttcaa
    30181 tctcaattta tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt ttgtaataaa
    30241 acgtcgtccc atccatttaa caaagttcat catgacataa aaaagccctt ttgttccttt
    30301 cagcaacagt ggcgaaataa atactgcgct tcctgtggcg aacagaccga caccgataaa
    30361 gaataatcca ctcagaatcg attgtccaaa cacactgatt cctgagatga tcatgaatag
    30421 gccaacacct aagatactga tacataaaac atagatacta aaaatgagcg ctagtaaagc
    30481 aaccaatagt cccccaataa ccaatataaa ccccagtgct agtggaatga ggacaggaga
    30541 agcaaataaa ccgataatga ttaaccaaat caggcgaatt tgtctttttg gatttttcac
    30601 taactcgttt tctgcttgtt cttcttctga aaagtaactg atttttaatt ttaaggcaaa
    30661 ctgctttggt gtaccatatt catcaatgat ctgatctaat gtttttccag aatctaaaaa
    30721 atattcctca taatactgga tcaaatcaaa acgatcgtct tcaggaattt cttgaagatg
    30781 atccacaact tcattcaaat attcattcat ttgtgctctc ctccaataac cggtctaaag
    30841 aatctcgaaa aacaatccat tcatcaataa tttcttctaa atgacgactt cccattggtg
    30901 tcaacgagta atatcgacgg tttctgcctt caaaaggttc atcataggtc tcaagatatc
    30961 cttctttttt caatcttctc aaaaccggat atagtgtaga ttcactgaca gagagacttt
    31021 cttgtacgat ttttgttaag gcatacccat acatatcttc cttgcttaat gctgctagca
    31081 cagtaccatc aagaagaaca gttggaatct ggatgtgcat tacgtcacct tctattctca
    31141 agtgtaatat tttatttcaa tctatattat acaacatata atatagtttc aaaatcagtt
    31201 tgtcaaatac aaaaaaactg actaaaagat ttgatccttt ttagtcagca aaaaatggct
    31261 tctctttgat ttttattata acagtggcat aatccaattt gtccacatcc agctcagtgg
    31321 catgataacg atcattgcag gtatcatgtc agctactggg aacattttca cgttgatcat
    31381 tctaaaaccg gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc
    31441 tggtgtagtc aatggataaa taaattttgc caataagaac aaaatataaa aaataatgaa
    31501 ttggggaatt gctatcacag aaacgacata ccctaaatta cacgcaaaaa tagctgctgt
    31561 gaaaaaatca aggacagatt tagaaatcaa aatcgtactg tctcctgtca tcccagaatc
    31621 taaacttcca tagatccccg taccacttgc acaaaataga acaatgaccg tcactaatgt
    31681 gctgataaat tcatcgtggg tcataccaag cttttcacta gggaagattt tagaaatagg
    31741 tacttgcatc agcgcagccc ccttatttat cccattacct aaatgaacaa tcaaaccaat
    31801 cgctgttcct atcactaaag caaaaataac agccggcata tttttcatcg gcgcaatgga
    31861 atagattccc atccccatcg aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg
    31921 ttcacttaat ttatttccta ctaatccgcc aattagtccg ccaaacaaaa cggaacctac
    31981 attgatcaaa actccagttg gcatcaaaaa actcctctct ttttctacga agcgaaaaaa
    32041 atcagaaaaa tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa
    32101 gattacacca attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata
    32161 tgatggatat ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca
    32221 aaactgctga tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag
    32281 aaacagaatt agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag
    32341 ctggaagctt attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag
    32401 aagtgcttga aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga
    32461 caatgatcaa ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc catcctgaat
    32521 ttacagtcca gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac
    32581 atagtgacac gatccacttt gctagaagtt tccaggcccc accaagttcg attgagtggt
    32641 tgcctactga aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta
    32701 aaaagaaatt ggatctaagc gaactgaaaa aggagaactt tttgctcctt ggccccacta
    32761 cgaacttgta tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct
    32821 cttataaagg agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg
    32881 ctattgttat ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta
    32941 tttctccaac aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg
    33001 cttcagatac attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt
    33061 ccatctatcc acccaaaaaa agagagcgac gcatgacttt cgctctcttt cttgcatcat
    33121 gtccttgttt caatgacacc tctaacattt ctcgtagttc aataagaaag cagcgatttt
    33181 tatgccaaaa gaaccgtaag aacatttccc taatcacaaa aaaacgacta aaaaccccta
    33241 aaaaccttca atagaatttt taggggtttt attctttttc ctattgttta cttaaccgta
    33301 tcattgctgt cgttcctact ccttcgatac tgctataatc aatacgtcca tcatgcagtt
    33361 ctagaatttt tttaaccagt gaaagtccta gcccattccc agaaatttga cgacttgtat
    33421 ctccttgata aaatttttca aacattcgat ctctggtctc ttcattcatt cctacacctg
    33481 aatcggtcac ttccagtaca atctctgtgg ctgtctcaaa tagtttgata tgaatctgtc
    33541 cattgatttg attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa
    33601 actcttcatt gcctgtgtaa tttactgcag caagttcgat atccaattct agctgttctt
    33661 tctcccattt aggctgaaaa aaaacgatta cttctctgat ttgttcatct aatcgatact
    33721 ctttcttttc cagttggata cgctggtttt ccagtttgtt caattttaag atgttctctg
    33781 ttaattgtga aagctgcgtg atactttcga taatccgata taaaaatatt tggcgttctt
    33841 cgtcagagag atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg
    33901 ttttgaattc atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga
    33961 gttcttggac cataacgttg aaatctttat acagttgctg tacttctgct actttctgct
    34021 gttcatccat tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg
    34081 cactgattgg atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca
    34141 tactgaaccc cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag
    34201 gcgagataga atgtcgagac aataagaaaa taaaaccgag aaaacacagc agtgtcacta
    34261 agatcgaaat gaagacaata ctagcaaaat agatccataa ttgtgaaaat agcttttttt
    34321 tcatctttaa ataaccgcct tatagcctag tccccgtact gtgatgattt gaaaatcttc
    34381 cgtcttttca aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc gttcttctgt
    34441 attcgtatct aatccccaaa tatcatccat caactgttgg cgagtgaaga tcttgttggg
    34501 ataagacagt aatttataca gaagcagaaa ttctttttga ggcagaatca acgaatgctg
    34561 atcttgtaac actgtataag catctagctc taattttgtt tgtccgatta tcaagctact
    34621 gctgtaattg atctttgaac gtcgaagcaa tgcttccaca cgtaggatca tctcattgac
    34681 atcgatgggt tttgtcatat aatcatctgc tcctaatttg aacccgcgtt tcttatcctc
    34741 aaaatcggcc tttgccgtaa tcattagaac aggtgtgtca aatctggtgc tacgtaatga
    34801 gtctagcaag tcatagccat ccattttcgg catcatgata tctgtaatga ttaattctat
    34861 acgtgtcgct tctagttttt tcagcgcatc ctctccattc aatgcggtga tgactgaaaa
    34921 gcccactcgt tccaacacag aacggtaaag caactgtaat tgcggatcat cttctactac
    34981 taaaatacga ttcattgatc tccctctgtt ttttgaaaaa tttctagatt ttttcttgta
    35041 gttcatcttc agttaacatt tatttcgtat actacgatca ttgttctgat atacttatta
    35101 gtataccgaa tatttcaata cgggagaagg gtgattatgc ttcaattaaa agatataaaa
    35161 aaatattata aagtcggcga aaccaccacc aaagcattgg atggtgtgtc tgtcgctttc
    35221 agacaaaaag aattcgtagc gattttaggt cctagtggtt ctggtaaaac gactatgctg
    35281 aatgtgattg gtggactaga taattatgat tccggtgata tggtaatcaa cggaaagtct
    35341 actaaagact ttaaggacag tgactgggat gcctaccgaa acaactctat cggtttcatt
    35401 tttcaaagtt acaacttgat tggtcatcta ggcattattg aaaacgttga acttgggatg
    35461 acattaagtg gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc attgcaccga
    35521 gtcggcttga caaatcatat gcataaaaag ccaaatcagt tatctggtgg acaaatgcag
    35581 cgagtggcca tcgctcgagc actagccaat gacccagata tccttttgtg cgatgaacca
    35641 accggagcgt tagatacaga aacaagtatc cagatcatgg aattgatcca agagttatcg
    35701 aaagaaaaat tagtgatcat ggtcacacat aatcctgaac tggcccatca atacgcagat
    35761 cgaatcattg aattttctga tggtaagatc ttaacagatt cccatcctca catcgaacga
    35821 ccaaaagatg atcaatttaa cttacgcaga acaaagatga gtttttggac agcgttaaag
    35881 ctttccttca acaatatccg aacaaaaaaa ggacgtactt tcttgacctc ctttgcttcc
    35941 agtattggga tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt ccaaaaacaa
    36001 atcgataaca cgcaagctga aacgatggcg aaatttccga ttactatttc taaggtaacg
    36061 actaaccaga ctcgtgacga tgccggattg ggtgcaagca aagcggatta tccagacagc
    36121 aaaacgatta ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa taagattgat
    36181 caaacatacg tcgattacgt aacagacatc gatcctaact taagtaataa tatcggattt
    36241 acccgtacta caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca gcctgtcagc
    36301 ttctcgaatc aaaatcctga tacggaatct ctttctttat ctagtacgat gtcagcaatg
    36361 acaggtgtgg gagtgtcaag tttccctaca cagcttgaca ctagcaaaga aaacttttta
    36421 aaagacaatt attctttact tgccggaagt tatccagcct cagcaacaga tgtcgtgctg
    36481 atcgtagacg gcaataataa tactaatatc aacgcattga agaatcttgg atttgatgtg
    36541 aaagaagacg aaaaacttga ttttgatgat atcgttggta caacattcaa attagtaaac
    36601 aacaatactt actatacaaa acttccaact gggaatttta tccctaatac agattatgat
    36661 gcgatgtatc agaatgcttc tgatgaattg aagatctcgg ggatcctacg cgtgaaatct
    36721 tcttctacaa tgaatctctt atctccagga atcgcttata gcgatcaatt aacgactcaa
    36781 atcgtcaacg aaaacaaaga atcagaaatc gtcaaagctc aaagagacag tggtgtaaat
    36841 gtcttaacta ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag ttatttaggt
    36901 ggagacagct tgccttcaag tatcatgatt tatccgaaca attttgaaga caaagaaaag
    36961 atcttggatt atctagatga ttacaataaa ggcaaatcag atgaagataa aatcatttat
    37021 actgatttag ctggaacgat gactgaactg actggcggat tgatggatgc tatcacttat
    37081 gtccttattg cttttgcagg gatctcttta gtaacaagta tgatcatgat cagtatcatt
    37141 acttatacat ctgtgattga acggacaaaa gaaatcggtg tactaaaagc tttaggtgca
    37201 cgtaaaaaag acatcactcg cgtatttgac gcagaaacgt gtattttagg tatttcttct
    37261 gggatattag gtgtgtttat tgcatggcta gctacgttcc ctatcaatag catcttatac
    37321 aacatgacgg atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat tattctagtg
    37381 atcgtctcta ctatattgac gatgcttggc ggtcatcttc ctgcacgaat ggcagcgaaa
    37441 aaagacgccg ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat ttagatttga
    37501 gtcataagag cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat atgttccttg
    37561 tttcgcgctg ttttttttaa ttatttttta taattctggt atacttttct ttcctttgta
    37621 ccacaactct tctggcgtat acctgtcttt tccatttatg aatactgttc catctgcttc
    37681 aatcgttact tcagtcccaa tccacatatt acgagaaagt gcgagaagat tttttctttt
    37741 tcctcttcct ttttttccaa attgcaaggc atcggttatc tcttctgtcc aatgagattt
    37801 cacgtatttt acttctccgt tttcaaatgt aactttcaaa atattcgcta tattttccac
    37861 ttttactgct ttaggaaatg actcctccat ttttttcact ttccttcctc caacactaag
    37921 tatgcgcttt cttttagtga taagtatatc atagtttatt ctattatcaa aaaaacgcat
    37981 ccattccatt gtagaatact ttaccgaaca agttgaattg ttttcctctt tttcaccttt
    38041 tactatagat aggtaaagac aactaaggag gaatctctca tgacagatca accgtatgat
    38101 ttaagaaaag tcatcgaaga actaaaaaca cttccaggtc aatatcacga aacagatatt
    38161 gaaatcgatc ctgaagcaga attatctggt gtttaccgtt atatcggtgc tggcggtaca
    38221 gtaaaacgtc cgactcaaga aggaccagct atgactttca acaatatcaa aggtttcccc
    38281 ggcacacgtg tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga aattttgcac
    38341 catgattatc gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc aatcaaacct
    38401 gtgaaagtag acaaagcaga cgctccagca caagaagtcg ttcatctagc taccgatgca
    38461 gacttcgata ttcgcaacat tttgcctgca cctacaaata ctcctgaaga tgctggtcca
    38521 tacatcacaa tgggtgtagt cttaggatca gatccagaaa aaacaatgag cgatgtaacg
    38581 atccatcgta tggttctaga agataaagat acgatcggta tgtacatcat gcctggcgga
    38641 cgccacattg gtcacttcca aaaacaattc gaagccttga ataaaccaat gcctatcact
    38701 atcaatattg gtttagatcc agcaattgcc attggcacga ctttcgaacc acctaccact
    38761 ccacttggct acaatgaact atggtgtgca ggagctttgc gtaacgaacc tattcaaatc
    38821 gttgatggta tagcagtcga tgaagtgggt attgctcgtg ctgaattcat cattgaagca
    38881 gaaatcatgc caaatgaaac aatgcaagaa gatatcaaca cgaactcagg aaaagcaatg
    38941 ccagaatttc ctggctatac tggggatgct aatccagctg tacaagtagt gaaagtcaaa
    39001 gcagttactc accgaaaaga caatccaatc atgcaaacaa cgattggtcc cagtgaagaa
    39061 cacgtatcta tggccggtat tcctacagaa gcaagtattc ttgccttggt cgataaagct
    39121 attccaggaa aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa attgatgacc
    39181 atcatgcaaa tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca agcagctatt
    39241 cttgctttat cagccttcaa agaattgaag acagtcattt tagtcgatga agatgttgat
    39301 attttcgaca tgaacgatgt agtttggact ttcaacactc gtttccaagg agataaagat
    39361 atcatcgtga tcccaggaat gaggaaccat ccattggacc catcagaatt accagaatat
    39421 aatcctggct ctattcgttt ccgtgggata agtgcaaaaa ctatctttga cggtacagtt
    39481 cctttcgata tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga agattgggaa
    39541 aaatatttga aataatgaat gatctgcaac tgttttgaca tcataaaaag gagtgcggaa
    39601 aatggaacaa ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg gcgcttcagg
    39661 caccatttat gccatcaacc tagtgaagaa actaaaagaa tatccgatga tagaaacaca
    39721 cggaatcatc agtccatggg caaaacagaa cttaaagctg gaatccgact tatcactaac
    39781 tgaattcgaa agcctgtttg attaccatta ttcaaacaaa gatttaggtg ctgctgtcgc
    39841 cagcggatca tttttgaccg atggaatgat catcgtaccg gcaagtatga aaacagtcgc
    39901 aggtatctct gttggtttag gcgacaacct gattgcacgt gctgctgatg taacaatgaa
    39961 ggagcaacga aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca ttcacttgga
    40021 aaacttgact aaattagcac gactaggtgt gcaaatcata ccaccaattc ctgcttttta
    40081 taatcagcct caaacgatcc aagatcttgt taatcaccat acaatgaaac ttttggatca
    40141 atttgaaatt gtaaatgagc attcaaaacg ttggcagggt gattaatatg gaaaaagaaa
    40201 cgatagttga agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa gtaatcggta
    40261 aagacttatt gatcacttta acaggcggag atacaccaca tatcggaaca gtcactacat
    40321 tttctcgtga tacagagatg caaactgtac gtttccctag ccacgatggt cggttccata
    40381 aagacgatgt cttagccgaa aaaatagctg tgatcatcaa aactgccgtt ccgggaagtt
    40441 gtacgatcac ttccggtatc catgttgacc atatctccaa tgcacaaatc aacgcctctt
    40501 ttccgatgtc agaagagtta ggttacaaga ttttagattg gttggaaaaa atagatttcc
    40561 atatcaaaga accagtttat tataaagatg gtgaaaaacc attatgaaag aagaggttgt
    40621 gaaatcagcg ttatgacctg agtatgagag aaaaaaatgg aaaaatagct tctcatattt
    40681 tgccgttttt gagctaatac cgagggacag ctgattgaac gccgtttatc aagaggttgt
    40741 gataaagccg cttcgtttcg agtagtaaga aaaattttgt aaacatgggt ttccacattt
    40801 ttgcataaat ttgacttact accgagaaac tggcttttga acaccgttta tcaagaggtt
    40861 gtgacacgtc ttttgtcaca acctctttta tttttgcttc actgtaactg ctacaatatc
    40921 accgatttct tttccgatgt ttttacgaat atcttttcgt agtccaagga tgtagcagac
    40981 tgaaccatct ggatttttca cgcccatatt cacgatactg ccttcatacg gcacattgtc
    41041 aaaagttgct tggactttaa ctcttccagc accgaattct tttcttatat catacggaaa
    41101 aataatatat gccccgccct tgccgacttc ggaagcatag atttttgatt caaaagaata
    41161 aatttccctc attttatttc tcctattttt tacatctagt atagcatagc aaaaaaaaga
    41221 aggaactgtg acaaaagtct tgtcgcagtt ccttttttcg aataaacggt gttctcctgc
    41281 caactcctcg gtcacaagtc acaatagtca acaaacatga gaagctgttt tttgaaattg
    41341 ctccttgtga ctcagagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa
    41401 agcttgcatc tacggcacta agcccaatca gatgaaaaat atggagtgct atttttatct
    41461 gattgttctt actgcttgat gcagagcagc tttttcacaa cctcctacat ggtgttcaaa
    41521 atattattcg ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt ggaattcagc
    41581 agctaaggct ttcgtacgtt gttcttgctc tgctttgctc atttttggat tttcagtcat
    41641 tgcttttacc ataccgtctt gaacataagc ttttgcttca ctttccatcg tactttgctg
    41701 tttcttcact aattcagcta acttggctgc ttggtccgta cttaattcta cccatgtctc
    41761 tgggattttg aatgtattca ctcggctgtc atattcgtga ttgttatcag caatattgaa
    41821 ccagaattta tacatgtcgt ttttgtattc ccaataagta gttttcgtca ctttttcagc
    41881 atcgccgtct gtcttttcta ctttatttgt ttcgttatcc gtaccagttg cttttggttt
    41941 ttcttgtttt tcatttgttc ggtaaagata cactttttct tttccattcc ctaaagcttt
    42001 atacaaaagc atatctgctc cttgggaatc agcactggaa acaatttttt ctgtttttgt
    42061 ttccgttact ttttccattc caaagtgatt ggacaaatta gcgacgatca aacttagtga
    42121 agcgacgaag attagtccga aaatcaaaga taaaaaggtt tgccatgttt ttttagcgaa
    42181 cacgttcgtt aaagcaaaag caagcaagct gacaatcaaa actccaataa tcatgcatta
    42241 tcccccgctt tcattagaac aggttctttt ttcttcaata aaaaggcaat aataaagcca
    42301 agaatcccaa agatgatcgc tacgataaac gcggcatggt aacctgacag tgtggcattg
    42361 attgcctgat ctttataagc taatggcgta ttatccaata cagatttgct tgggatattt
    42421 gcttttgtca cattcgtcaa cacactgatc aaaatagctg taccaatcga gcttgcgatt
    42481 tgacggaatg tattatttac cgctgtccca tgactgatca agttatttgg caatgcattc
    42541 ataccagaag tcgttaccgg catcatgacc atggagatcc caaacatacg gatcgcataa
    42601 aggatcacga tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcc
    42661 gctgtcaaaa tgaacatccc tgtgatggct aaacgtttcg cgccgtattt atcgaaaatc
    42721 gcacccgtca caggcatcat agcccccatg atcaatgcac ccggcaacaa catcaagcca
    42781 gaatggaatg ctgattctcc acgaatattt tgaatataca atggtacgac catttctgcc
    42841 cctaccatcg ccatatttgt tacaccagct aaaacagctg caatcgtgaa tgtagatgat
    42901 ttgaatacac gaagttccaa gaatggatgt tccaaatgta attgacgcca tgagaacaag
    42961 gcgatgaaaa tcacaccaac aactaagaaa ccaattactt tcaagcttcc ccagccgtca
    43021 tctcctacac tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga taaaatagcg
    43081 gataaaatat caatgctagg atttgacaat ggaataacac ttttcattag gaagaatgct
    43141 aaaaccaaga caagaacaac gattggcagt accataccaa acaagtcacg ccaatggtag
    43201 ttatcaatga tataaccaga taatgtaggg cctaaagcag gagcaagtcc aataacgatc
    43261 cctgacatcc ccattgctga tcctcttttt tctggcggga aaatagatag catgatattt
    43321 tgcaataatg gcatcgatac tcccacaccg caagcttgga tcaaacgacc gattagtaat
    43381 gtactaaaat taggtgctgt aaagcaagta atcgttccga tcaaaaagat cgtcatggca
    43441 gaaagatata aatttcttga actgaattta ttgatcagcc atgctgttat tgggatcatg
    43501 atcccgttga ctagcaagaa accagtcgtt aaccattgca catcagacgc tgaaatatcg
    43561 aaagctttca tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa aacggtacaa
    43621 aatgtaccaa ttaataagac aacaactaaa agtgtccgat tatatggttt tccgtaaata
    43681 tctacgggtt ggttgttact catatttctc cttctttctg aattttttct gaaactacta
    43741 gaatacttta gtcctaaatt cgctatttgt caacctactt gacacataag ttaatacgga
    43801 tataattaag ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg cttcccttaa
    43861 acgtttgttg gaaaatgatc aattggttgt tggcatcagt gaattaagtg atatcgtagg
    43921 tgtgtctcca cgtcaattaa ggtattggga acaaaaggga ttcattcgtt cgatcgaagc
    43981 tgatgcgaat tgtcctagaa aatatcgttt acctacagtc ataaaagtag aactgatcaa
    44041 aaaatattta gatgacggat ttactttgtc aaaagctgca gaaaaggcag aaatccgcca
    44101 aaagaaaatg caccatgtcc gtaaagtttt ttcaaaagca atcaaagata tcgaattgat
    44161 cgacgaacgc tatacgattt tacaaatcgg taacttcaaa gaagagaatg ctaagatgta
    44221 catcattcat gacaacgaga cagacgaatt aacttacaag ctccttcctg tcgatgaaaa
    44281 agctgactac gaacagttgt taaaagaact ctaaacgcca ctaatctcct aagtaccaag
    44341 ctttttaata agtagatgct acataaatac ttgaaaaaag gcctgtttga acataagaat
    44401 atccgaacgg tgatctgcgt ttctttcata tagaaaggaa ttctccatca ctgttcggat
    44461 atttttttat ttttttcggt tactttcttc taatacatct gttagccaat ttgcatattc
    44521 tgtttcccga ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt catgtttttc
    44581 tttttcagta ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt tttcttcatg
    44641 aaggtcacgc tgctgctgaa taatttctac taatgccgga tcattcacgt ctttgacgaa
    44701 atacaatttc agaacaaatt cgtctcgatt aaccggtagt tcattggtgg gtgtatgaat
    44761 ccactctgcc aattgttcct tcccttcttc tgttaattca taaactttct tttccaattt
    44821 ttcacctgtg atctctactt gatgctgaat gattccttgc tcttccattt ttgcaagttc
    44881 aggataaatc tgactatgtt ttgcttgcca aaattctcct atttcatttt tgaacgattg
    44941 attcaattca tatcctgtca tcttcttctt acttaataac cctaacaaaa tatatcgaag
    45001 tgtatttttt cttgccatca gttttccctc cttgacaaat ctttaaatcc attctatcat
    45061 aaatgagtaa tcgaaaacat gtagtttatt acatgtttta atttataagg agatgcttaa
    45121 acatgaaaga atttaaagaa ttggaagatt ttattggaac tcactttatc tatacgtatg
    45181 acaatggttg ggaatatgaa tggtatgcca aaaatgacca tactgttgat taccgtatcc
    45241 atggcggaat ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc gttaaattga
    45301 cggacggcgt attcaaaatc acttggacag agccaacagg gacagacgtg gcattggact
    45361 ttatgccaaa tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg gttgaagaac
    45421 atccagaaat cactgttact ttccaaaacg aacatatcgc agaaatggaa gcagctcgtg
    45481 aaaaatatga aacttatcca aaattacttg ttcctgaatt tgctacgatc acttatatgg
    45541 gtgatgcagg tatagacaat gaagatgtta ttagcgaaga accatacgat ggaatgccag
    45601 atgatatccg tagcggaaaa tacttcgatg aaaattataa acgagtaaaa taaggttgtg
    45661 aaagagaggt tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa cggaaaaata
    45721 gttcctcatg ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
    45781 tatcaagagg ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta gttagaaaat
    45841 agcttttcat attttctagg ctagtgggct tattaccgag aagcagccgt ttgaacaccg
    45901 tttatcaaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaatggaaaa
    45961 atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc
    46021 gtttatcaaa gattgtaaca actgcatatt cacacagtta aattagtgac ttcaaaatga
    46081 aataatgctc ccagcaaaaa attaggctgg gagcattatt tcgttattct gttcatcaaa
    46141 aatcagcgtt ttgattccat ttcatcacaa gttcctcctc atttactgct tcatcttttt
    46201 gctgtgtttg aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat tgattttttt
    46261 gatagactgt caaagtcagt gttttattgg tttcttttat ttttttcatt agttgcgtac
    46321 cgattctttg ttgccggtat tcccttttca caaagattcc tgcgataaac tgatcaatca
    46381 ccccagcaaa accaataatt tcttgatcta tttctgctaa gtaaatcact gcatctggta
    46441 aagctttcct aacagattct acttgattct cccaataagt tgatggaatg aattcatgag
    46501 aatccaagtt agcatgcagc caaatcgata gaacagattc cagttctttg gcagacaatt
    46561 ttgttgttgt gcggatcatt ttttctctcc ttacttattc attgatttta aaataaaaac
    46621 catttttttg aagctctttt acaagttttc gcccctcaaa aaagtatatc ttctcttgtt
    46681 ttacttttct aataccaaaa gaaacagcaa ctttaggatc atcagctgaa acgattcgat
    46741 aagttccacc aggcaattga tccagcttga ctttcccatg atggtcgctt tgtactgaaa
    46801 acggcacacc atttctagta agtgtttttt tcttatgttt caaaatgaat gaactaccat
    46861 ttaccggttt ccctgacgaa tcactaataa taaatgaaaa atcaagcact tttttactta
    46921 atctatactg atagattagt cgtccgataa acactaacag tatgactaca gctattatcg
    46981 ttcctgcaag aataaaccat tgccgcatgg ttctttcctg atttcctttt ccttcttctt
    47041 tttttaccga ttcgttataa ggaatccgat gtcctgtcac tagcaaacga tgcgtgttga
    47101 tcatatacgg cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa atcgttagaa
    47161 cagacgtgtc atctggcagt accacttgta tatttttaac ttgataagcc aatttctccc
    47221 ccaaaacagt caaaataaac gtgtctccgt tttctaaacg atctaattcg gtaaataacc
    47281 gtcgattagg taaaccgctg tgagcagaga tcactgaatg cgtcgaattt ccaccaagcg
    47341 ggaaagaggt tccttgcaag acgcctgctc cattttcaag gatcctacca ttgagccgat
    47401 cataaagtga aatatttaat tggatcttag gtatattgat acttccgatc agccattcct
    47461 ctaattggct ggatgttgta ctttcatttc tgttcttttc attgaaagga tcagtaccag
    47521 gattgaatcc ttggtcagtt aatcgttggt ttttctcctc cattttcttt cgctgcactt
    47581 caatttcagc tgcggtccgt ttttgtactt gttcgatacg tttttgatca ataaaactat
    47641 ttaaagaatc cacataaaat ggatataaag cgatcagtaa ccctacgacg atacatatta
    47701 ataaaaacaa acgaaacaaa agtcgacgag actgtttttg cttactctgc ttcacatgtg
    47761 tcctcccttg caagtttttt tgttctttct tttattatat gggttcagga aaaaaataga
    47821 aaatgaaagc cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa gtacagccca
    47881 aaaaaatagc tactcatatt ttttgttggg ttgggcttat taccgcagat gcaagctttt
    47941 gaacaccgtt taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa taagagcgct
    48001 agtcagaaaa tagcttctca tattttctag gctagtgagc ttattaccga gaagcagctg
    48061 attgaacacc gtttaccgag aggtcatgaa aaagctactt tgtttcgagt attaagaaaa
    48121 attctgtttc aaaacaagga gatgcaacaa aacttatgtc gcatcctctc tccttagaac
    48181 tttcacttat gtttttcttg taaaattcat aaatattttc atgaattttc taatttcttg
    48241 cctacctctc gcataggata tatgatgaat ttatctaaag tttgcaaagg agttcgacaa
    48301 ttgaaaaaat atggcccatt tttagcaatt tttttcattg ctttggcatt tctcatgcct
    48361 catttattca cacataaact aattttgggt gctgatgccc cattccatta caatcggttc
    48421 tatgatgccg cgcaacaaat taaatatggg gatttccatt attttccttc cctttatgga
    48481 ttccagcaaa gtggcaggat cgtgaatgcc gtttacggcc catttttcgc ttatttacaa
    48541 ggattgatcg tgttgcttgc aggtagctgg tttcattatc agattctttc aaatttcatt
    48601 ctttatctta tttcaggatt ttctatgctg attttactga gatatgtcaa tgttcgaaat
    48661 tgggtaagtg taccagtagc cgttttattc atgagtacgt attctattca atactggtct
    48721 atcaaccaag gattcactag ctgggggact tgtttttatc ctttatgtat gatccctatt
    48781 gctgatttcg tccttcataa aaagttccct gttatcaaag tcgctgtctc agtgggactt
    48841 atgacacaga tccatttact gtcttcgatc atgttagctt tgatgtatat ccctttttat
    48901 ctttattact tttttaacca gactgaaaaa ggaaaagcac tgcttcaact gattaagtcg
    48961 gtcttgcttt attttgttct gacagcaaat atttgggcag gattagctat tgtttacagt
    49021 gggaataaga ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc gatagatctg
    49081 gatggcagtt attggctcca ttaccctaag gcctttccta tattgatatt atcaggtttt
    49141 ttgcttttta ttttctttca acaaaagaac accttattcc aaaaaatcgt gttgtacaca
    49201 agtcttgttt tcttttttct ttctactagc ttgattccat ggacgacatt gatcgaaaaa
    49261 aatgtgcctt ttattagttt gatccagttt ccatttcgat ttttcgtgcc atttacgcta
    49321 ttgcttctac tttaccttgc cctttcactg aaccagtggt ctgaacaaaa gtggttccgt
    49381 cttttaagta tatttggcat tcttattttt tcagcacaga cgatccagaa cttataccag
    49441 catctggaaa agtgggaaaa tgaaacattt gtcagtcgac atacttatct attcgatacg
    49501 ccggaagaag caaggaaaac attcttcagc aaagatatgt ctaccagctt gttcgttttc
    49561 caaaaaacca cacctgacta tctgccaatc tataaggaga caaaagacaa taaatacgac
    49621 cggtataacg aagaaatact agaaaacgaa acaactttta taaaaacaca tccaggcggt
    49681 cttttgacaa tcaagtggaa aaacactggg gacaaaactg ttcatatccc tgttatcgtc
    49741 tacgatcgga ctgtgctaca gcaaaatgga aaaacattga ctgattatga agtaacagat
    49801 atcgggaccc caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct tcattatcaa
    49861 acgccaatct atttctattt cattctttct cttactttga ttggctggtt taccttgcta
    49921 tgtttattta tttatcatag atacaagctt ttacgtgctt aggcaaaagg gataaataca
    49981 tccgatcaaa aaactctcca aacggtataa tcgtttggag agttttattt taatcagttt
    50041 ttttatgtta tatgttatat tttttattcg ttcacatcta attcgatttc caccccgtag
    50101 tgatcactga tgattggact atcgatccca tcaaaaatcc gttgatattt tttaacctgc
    50161 attccttcag ggacgaaaat aaaatctata cgcaattttt ctgtattttg ttcccaacca
    50221 tctattttct tttccacagt agctgctttt cctttttcat tcgcgacaac atacgaatct
    50281 tgaagaagaa ggcaactttc tcttacagta taataacctt gagagtctac tggattattg
    50341 aaatcaccta ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc caagtttttc
    50401 cactcaaaat aaaagccact ttttttatct gtccaccaag aaaagtggca gctcgagaca
    50461 aaaatgtcct gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt ctgataatct
    50521 tctttctttt gaaattggga gacggtaagg acctgacttt ccaatgtttt tttcgtaagc
    50581 agtgcatttc cttcttcata tttatcataa ccgatgtggc tcatttccca actccaaaaa
    50641 tactcttttc cgtgttcctt taaaagctga actaaacggt aggcaaaatt atctttacgg
    50701 ataggtactt gattctttac cggacagaac ttcattagct tcgcaggttc tacttcttca
    50761 gattccagta attggttgat ttcttgtaaa gcaatcactt cataatcttc taacaggatc
    50821 ttatctgcta attcttgcaa ttttttctct ggttctggct caagccagct atgcgtgttg
    50881 agtgtcatga atttcattgg ttttctcctt gtcttcattt cgaaacaaaa gggttgtgac
    50941 aaaatttttg tcacaacctc tccagcaaat agtgttctcc tgctaactcc tcagtaataa
    51001 gctcaatgga ccaggagcta ttttttgttt ggctgttttt atcacttggt gcagggcagc
    51061 tttttcacga cctctccact aaacagtatt caaactgctg cttctcggta ataagcccaa
    51121 ccaaacgaaa aataggagga gctatttttg tttggctgtt cttatgactt gatgcaggac
    51181 agctttttca caacctctcc ttacatttta atggagccga tcactttacc agctgtcact
    51241 gtacctaatt gcttgatcac tacttcttct actttatcgt ttgtaaatac gatcatgatc
    51301 gttggatctt tttcttcgtt ttggatcaca tcaagatcca tctcagctaa tttcgttcct
    51361 gttcctactt cttggccttc tttgacagca atctcaaaag ctggctgatt catttggact
    51421 gtatcgattc ccatatggat caaaacttcg ataccatctt tcgtttccag cccaattgca
    51481 tgtttgctag ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat
    51541 gttggaataa cagcaaagcc gtcacccatc attttttgtg agaataccgg atcttttact
    51601 tcagataaag gaatcacttc tccagaagct gcggaaacta gttcttgttc tatacctaag
    51661 tattgcttac tacctgcttg tccatttcct gtagaaacca tttctggctc tggaatctct
    51721 gctcctgatt gcagcagatc ttcgatatct gatttcaaga catctgcttt tggtccataa
    51781 actgcttgga ctccattgtc tttgacgatc aatcccatcg cccctgctcg tttccaggct
    51841 tcttcagtcc ctactttttc tcgatcattt acacttacgc gtaaccgagt catacaagca
    51901 tcgacatctt tgatattttc ttttcctccc agcaaatgaa tgatctggac cacttgctga
    51961 tctgctgttc ctgttccgct gttagataca acggtttctt cactgtcatt gtcgtaattt
    52021 ccgttacgtc ccggtgtagc aaagttgaat tttttgatca agaaatttgc taagaaatac
    52081 gtaacaacac caaaagcgat cacacacaac acgaagttga taagatctcc acctagaccc
    52141 gctttgatag ctaacggtgt ccgagtcagc aattcgatat tcccaaatga atggacccgc
    52201 aatggcaaga tatctgccat cgcaaatgca gccccttgga tcactgaata aatgacatat
    52261 aatggcacag cagcaaacat aaacatgaac tccaatggtt ctgttacacc tgttagaaag
    52321 acagccaacg ctgctgaaaa atacattgat ttatatcttg ctttcttatc aggatcgaca
    52381 ttacgataca tcgcaaaagc cattcccatc aagatacctg aagaaccaat catttgtcct
    52441 actttgaaac gagccggcgt ccagttatgc aacacaaact catattgcga catatcccct
    52501 gcacctttaa gattcactaa atcagtggcc caagccaacc ataacggatc ttgcccaaat
    52561 acttgtgtcc ccgcctgtgc gcctgataaa atctcatacg atccgccaag ctgtgtatag
    52621 ttgataggaa tcgtcagcat atgatgcaat ccaaatggca atagtaaacg ttctaatgtt
    52681 ccaaataaaa acggtgctag aatcggtgcg ctttcttgtg attctgcaat ccataaacca
    52741 aaattattga ttccagcctg tacgtttggc cacaccacag caagaatcaa tgccacgatt
    52801 gttgaccata agataacaac aaacggaaca aagcgctttc cattgaaaaa tgacaaagca
    52861 tccggcaatt ttctataatt atagtactta ttataagcca ttgccccgac aaatccagcg
    52921 atgatcccta cgaatactcc catattcaaa gctggtgctt ccaatacact tgtaaaaaat
    52981 cctttaacca tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc attcgcaagc
    53041 atgtcacttg tgacaccaaa gatagcccca gtgatccgat tgattaaaat aaatgagatc
    53101 ccagctgcaa atgctccgcc tgctctttct tttgcccaac ttcctcctat tgctaaagca
    53161 aataaaatat gaagattacc aataattgcc caaccaatat tttcaattac tccgcccgtt
    53221 gtgacaagcg gcgtccaatc aggattgatc aagggaattg atttccctat actgatcatc
    53281 aacccagctg ccggcataac tgctaccaca accattaaag cctttccaaa tttttgccaa
    53341 aactcaaaac taaatatctt tttcattccg tttagctcct ttacgcaatc ggtttcgtta
    53401 aacgtattat agcgtgcgtt ttcttttttg taaatgcttt attttttatt tttctgtcta
    53461 taccattcta taaaacctat aatacgtgtg ttcccttcta taattttctc ctaaaaattt
    53521 atttttttaa gcttttttct atgaaatatt ttcttttgtc tattgacttg tgcaatcggt
    53581 tgcgtttatt atgaacgtat aaaacagctc attaggagga gaaaaatgaa acagatcaaa
    53641 cgactatttg ctattgatcc atggaaaatc agcacgacac aattagataa aaaaaatctt
    53701 cgactgcaag aatctttaac aagtattgga aacggttata tggggatgcg cgggaacttt
    53761 gaagaaactt actcagggga ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc
    53821 gataagacac gtgtcggctg gtggaaaaat ggttatccag aatactttgg aaaagtgatc
    53881 aacgccttga actttatcgc gatagatatt tcgatcaacg atcagctgat cgacttggct
    53941 tctttggaac cagaagactt ttactgggaa ttagatatga aaaatggtgt attatctcgt
    54001 acattcacaa tcactacttc tactaataaa gtacgcattt ccttcgaacg ttttctgagc
    54061 attgtcaaaa aagaatcagc ttatatctat ttaactatag aaatgcttga aggtagcgga
    54121 acagtcaaac tcgtatcaaa attggatgga aatgttcaaa acgaagacag caactatgat
    54181 gaacattttt ggaatgagat tgatcgtgga actatagaag aaatcggcta tttgacgact
    54241 aaaactattc caaacaactt tgggattgaa caatttactg ttactgcagc aatgcgccat
    54301 ttcgttaatg gaaaatcagt catccctgac tatcacgaag atactttagc attacatggt
    54361 tgcttgtctg cagaattatc tcaaggtgag acatggtcag tggaaaaaga aattatcgtt
    54421 cttactagta gagatattcc agaagcgcag caagcagaaa aagctgtctc agaattaacg
    54481 aaccagacaa acaattatat ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga
    54541 tggaagcttg ccgatgtggt catcgaagga gacgaagaag cacaacaagg tattcgattc
    54601 aatttgttcc aactattctc tacttactac ggcgaagatg aacgattgaa tatcggcccc
    54661 aaaggattca caggagaaaa atacggcgga gccacttact gggatacaga agcgtatgct
    54721 gtccctcttt atcttgcttt agccaaacct gaagtaacta agaatctgct gaaatatcgg
    54781 cataatcaac tgccgcaagc aatccacaac gctcagcaac aaggattaaa aggcgcgctt
    54841 tatcctatgg tcaccttcac tggtgtcgaa tgccataacg agtgggaaat cacattcgag
    54901 gagattcacc gaaatggcgc gattgcctat gcaatctaca attatacgaa ttacaccggc
    54961 gatacggaat atttgaaaaa tgaaggactg gaagttttag cagaaatcgc acgcttctgg
    55021 gcagaccgtg tccatttttc aaaacgagca aataaataca tgatccacgg tgtgaccggc
    55081 ccaaacgaat atgagaacaa tatcaataac aattggtata caaatacgat cgcagcttgg
    55141 gtcttgcgtt acacgcgaga aaattattta acttatcaaa aagaagctgc tatcactatc
    55201 agcgaagaag aactagccaa atggaaagat atcgcagaga acatgtactt cccagttgac
    55261 gaagaattag gaatctttgt ccagcatgac acattcttag ataaagatct catgcctgta
    55321 tccgatattc ccacaagcga tcttcctctg aatcagcatt ggtcttggga caaaatcctt
    55381 agatcttgct tcatcaagca ggcagatgta ctacagggga tttatttctt caatgatact
    55441 tttacaaaag aagaaaagca aaaaaacttt gatttctatg aaccaatgac tgtccatgag
    55501 tcttcccttt cgccaagtat ccattcgatt ttagcagcag aattaggaat ggaaagcaaa
    55561 gctgtagaaa tgtaccagcg aacagctcgc ttggatttgg ataattacaa taacgacaca
    55621 gaagacggct tgcacatcac ttccatgact ggtagctggt tgtcgattgt ccaaggattt
    55681 gctcaaatga aaactgccca tgatcagttg agattcgcac catttctgcc aaaaacttgg
    55741 aaagcttatt ctttccatat caattatcgc ggacgtttat tgttcatctc agtcacagat
    55801 gaacaagtta ctttcgagtt attagaaggt tctcctctag aaatttacgt ctatgaacag
    55861 tcttattctt tagcggatcg tctagaaata acgctaagaa aggaggaagc aaatgtttaa
    55921 aggggtattg tttgatttag acggtgtcat taccgatact gctgaattcc attatcgtgc
    55981 ttggcgaaag cttggtcagg aaatcggcat tacgatcgac cacacattca acgaacagct
    56041 aaaaggcgtc agccgagaag actcactctc tttgctttta gcttatggcg gaaaagaaca
    56101 ctctttttca aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt attatttaga
    56161 gatgatccag acgatcgaac caaaagatgt cttccccggg gtcgtgcctt tactggattc
    56221 tttaaaagaa gaaaaaatca agattgctct tgcttctgca agtaaaaacg ggccattcct
    56281 acttgaaaaa atgggtctta caccttactt tgatgctatt gccgacccgg cagaagcaac
    56341 aaacggaaaa cctgcgccag atatcttcct ccttgcagca aaagctgtcg gactaaaagc
    56401 agaagaatgt atcgggatcg aagacgcgca agccggtatc caagccatcc tctcaagtgg
    56461 cgctcaacca attggcgtgg gacgtgctga agaccttgga gaggagatcc cgcttgtccc
    56521 tgatacgact tttcttacga tcgattactt gaaagaaaag tggcatgacc atggataatc
    56581 cagagatctc cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg
    56641 actacttaga agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag
    56701 acaaagaagg aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa gatgttctaa
    56761 cagacaaaag cttttttgga gcaactgtcg gacctgtagc cggaaggatc cgggatgctt
    56821 cttggggtga gcatagattg gagaaaaact gcggatcaca ccatatccac ggtggcacga
    56881 aaggctggtc tttccaattt tgggaagtag aagttttcaa aactccatca tcaataggtg
    56941 tggtctttta cttaaaagat gaattttcta cctatccagg accaatcact gctacgatca
    57001 cttaccgttt aacaaaaaac gaattagaga tgacaacctc tgctagtagc cttgttgaaa
    57061 ccatctgcaa tccgaccaac catgcctact tcaacctttc aggaaatgga acaagagaca
    57121 tctatgaaca tcagttaacg gtctttttag atggtgtgct tgaattagat caagaaaaac
    57181 tgcctactgg aaattggaca aaaaaagaaa aactgccaat cgactttaga aaatccccaa
    57241 cactgcaaga gatccttgca tgttatccag atggactaga tgatgtattt cttttgcatc
    57301 atcctaggct ttccaaaact tccttacaat tattcgagaa gcattctggt cgccaaatga
    57361 ctatagcaac ttctaataaa agtatggtat tattttctac gactggattc gaagctgatt
    57421 tctctgtcaa tggaaaacag atgcattcaa actacggatt agcaatcgaa ccgcaagaaa
    57481 tccctgatat cgttcatttc cccaaatttg gatcgatcaa cctccatcct ggtcaagaac
    57541 ggatcagtca gacgatttat cggttctctg cacaataaca agagaaaggt gtttttatca
    57601 tggctattac cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg accgtctctc
    57661 gtgtcatcaa cgatcatcct agtatttccg agacaacaaa gaagaaagtc cgaaaaatta
    57721 tggatgaact aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca
    57781 gtgccatcgg cgtaatctta ccccctcttg attcaaaaga gcggatcggt aacccatttt
    57841 atcttgagat catggaagca atcaacgaag aagcgcggaa ttatgatatg actacagcaa
    57901 tcgctactgc taaaaatttt gaattgctgt tagagaacgt ccagcgtatg catttacaaa
    57961 aacaagtaga tggttttatt ctagtgtatt cagatagtca agatactgtc attgattatt
    58021 tataccaaaa ccacatccct ttcactttga tcggacagcc ttatcatcat gagaacgaaa
    58081 tcgtttatgt agataatgat aaccagcttc taggtaaaca agcaacagaa tttttgatcc
    58141 agaatggaca taagaatatc ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg
    58201 aacgatattt cggttatcaa aaagcaatga tgatggctgg tcttcctgcg ttatccagtg
    58261 tagtactaga gcatccggag gattatctgt cctttgagaa aacgttgaaa gaaaacgaag
    58321 ccacagcatt ggtggtcatt gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa
    58381 tgtatggcta tgtcattcca gagacactct ccgtcattag cttcaacaac tcgatcttct
    58441 caacattaac acacccttac cttacaagta tcgatatcga tatctccgaa ttaggacgtg
    58501 tcgctatgca aaagctgcgc gaattgatcc atgaagaatt gagcagtggc gtacgtttgg
    58561 tcatccctca tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac ccatactaaa
    58621 tagctgactc actaaatacg ctgaccattt atcctatggt cagcttttta tgtaaaagaa
    58681 gatactactt ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac
    58741 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa
    58801 ataagctgaa attcacaaaa atttaaaaag caattttctt gagtttcttc ttatttcttg
    58861 gagttaaacg cttctgtccc agcctctttt ttccttcctt caatttttta cacgtcgacg
    58921 attcaataaa aaaccgccta atattgtaaa gacaggtgca agaatcgaga aaaaggcgaa
    58981 tggcaagtaa tgcaacgcgc taactttcat cgttcccata atgaatgtgc cgctgactcc
    59041 ccacggtacg agagaattga cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt
    59101 ttttgataag ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa gaatgatgga
    59161 cagatactgt tctccaacga tcagattgat tccgatacag ctcaaggcag taaacccgac
    59221 taaacgagaa ggtcggtcca tcttttcttt taattcttga acgatggtat caacgatcag
    59281 atatttgatc agtaacccac ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa
    59341 catactcatg atcccgcctc gagaaagcag ttcatctagc tttggatcag gtgtatttgc
    59401 gacataacca ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt tctcatggat
    59461 aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat gtagggatag ccggaatttt
    59521 tttgattgcc cccccaaata aaataactag tggaatcaat gtccaaggag agatccaaaa
    59581 gctcgctttc atgttttgga tcatttcatt gatactagct agatcggctg aatgactgtt
    59641 atgtcctaat atcgtataaa aaatcaacga aaggacgaat gctgggaaat ccgtataacg
    59701 cataccagcg atatgttcga atagattaac tcctccaata cccgctgcca agttggtcgt
    59761 atctgataaa ggcgagcagt tattccctaa aaaggcaccg gaaacgactg cgcctgtagt
    59821 cattgcatca ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc caatcgtcga
    59881 aatcgttgta aatgaactgc cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt
    59941 cggtaaaaag aaacgcacag aaacaagttt aaatccataa accataatcg tagggatggt
    60001 tccagataaa atccatgaag caactaagac accaatcaaa agaaaaatca ggattggaat
    60061 gatccctgga gtgattcctt tgatgatccc gtcatggatc tcatcccatg tgaacttttt
    60121 aaatctgcca taaaacaaca aacacatgaa agctgcaagg atcgggatat gtggcgagag
    60181 ctggaatccg atgatcagta ctcctaggag tgccaacaac acgatcaata cagcgatact
    60241 ttcttttaaa gaaatatttt tattcatcgt cgatctccta tttcaaatga aaaggccctg
    60301 ccatgaaaaa actattcact tggcaggacc tcctactaaa tcagcgacaa aacgtcggct
    60361 gaaagactta tttttttatt tttcttctaa acttttttct acctcaatcc cccaagaatc
    60421 tttcttgaag tgattgatga tcaatggaat aacgaacatg accactgtga tagctgctac
    60481 taataatgca tcactcaatg tcgctgtacc taatgtagca aatacgacaa cgatgataga
    60541 gaagatcagc atgcagctga ctacccaagc taatccgttt ccgtttttac caattcggta
    60601 aggacgttcg aattctggat gtttcttacg cattttaagc aatgaaacag caatcaaaat
    60661 gtacacgata caataaataa cagttgtagc atttgtaagt gtcaagaata cagcacttac
    60721 gtcatccatt acaccataaa gcaaagcaaa taatgaaata acgattgatt gtgttagaac
    60781 gacatttcta gatacgccgt atttgttttc tttatggaat ccgaattttg gtggaaggaa
    60841 tccttcacga gctacttgga tgattgtttt tgatggtcca gtaacccaag ctgataattg
    60901 taaaagcaca ccaataaata ccatgaagct gaagatattt ccgatcactt ctggtaagcc
    60961 taggatttga cagtcaatca agataggctg cgtgatattt gctaattcca tttttccatc
    61021 tggtacgaca tttgctacaa gcatcgcatt gatgacattc aataaaacta atccgatcaa
    61081 agcgataaat acgccttttg tataattttt tgttgcgtct ttcaaacgag gcatgtaaac
    61141 agaactcatt tcgatcccaa caaaaataaa tgtgatacca gctaagtatt tcaatgaatc
    61201 aatatgttcc aagtcaggga aagcttttga ccatgaaaag tcacctagat agccgtttga
    61261 gactagtccg actttgaagg ctgccaaaac acctaaaacg aacatgatga ctactgggat
    61321 atatacccct agccatacac cgatgttccc gccgactttt gccatatcga atttcaagtt
    61381 caagatcgtg atgatccagt agataactaa aatacaacct aaaacgaacc agtgattatt
    61441 gccaagttct acattaccaa atgtgtttcc taacaatggt cctaaagtag aagcgaccat
    61501 taccatacct gggaacattt gcacccataa tagccatgca acgacaaatc cccatttgct
    61561 gcccagtgct gtttttaccc ataactgagg accgccttct tgaggaagca tcgtggaaag
    61621 ctcaccggaa accattgata tcggaccggc aaaaaacaaa actgcaaaaa ttgaataaaa
    61681 aatcaatgtc caaccgactg cggctaacgt aggaatactc cgaaccgttg cacacagtgc
    61741 cattgtcaga ccgataaagg tcccaagacc cattttactt acttcagatt tactcaccgt
    61801 tgacactctc cttgattttt tggataaaat aacggtgtct ttgaagtagt ttcagcaata
    61861 aacgaaacaa ttcagcgaat atttgagggg attctttgag tttattgctt gaagggtttc
    61921 ttcaaagaca acctcaaaac aaagggatcg aagagcttag gtctcccctt gctcttcaga
    61981 tccaaacatg cgtcagaaac agttagtatt caagctgttt tctaacatga ttaccttgct
    62041 tattttgctt cgcttgccaa taattggtct gcgtagattt tttccaattt ttcttgaaga
    62101 gctgctttga ttttttcagc atattcttca aagtgttctt ctttgttcat gtatggtgtc
    62161 attacgctgg cacgcaatac agttactttt ccggcacggt tccattcttc atctgagaat
    62221 cctagttggt tcacaaattg taatgggctg ttgccatagt ctgggatagc aaagtcagta
    62281 tgtgaagtta agaactcatt accgtaaatg cttcctttca cgtatgaaga ataatcatat
    62341 acgtcatggt tcaatttatt cattgcaact aagtcatcgt tgcctttttc tttgaataca
    62401 tagtctacca tattgaaatc tggataagtt aatggatgaa cttcgatttc tttgtctcct
    62461 actttaaatg acaagtcatt caagaagttg tagaaacgat gtgcaccttc gattgatgca
    62521 cccattaatt taccatatcc tgtgacattc aaaggtaaga catggtgtgc tgcccaaacg
    62581 ctggctgctg ttgcccctgc ttttgaacct tccaagatat aagccccaag taatgctggg
    62641 atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag agataacgtc tctcatgcgg
    62701 atatctttga tgacgatacc accggcagaa tatggaacat agcccatttt atgtgggtca
    62761 atcgttacag attctgcttc ttcgattgct ttatatgcac tgtgtacttc ttctaagata
    62821 taatctttgt tttctgtaaa gacattgtat ttgtaatgaa catcttttaa atcttcaaat
    62881 ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc ataagcagca
    62941 tctacgtgta agtagaagta aataccgtct ttttctaata cgcgacgcaa tgcaacgatt
    63001 ttatcgatac cgtcgattgc tccttcttct gttgatccta caacaccgac tacacctagg
    63061 attggtgttt tttcagctgc tagaccacga acgatttttt caagttcgtt gatgtccatg
    63121 cgatagttgt ggtcaactgg cactggaatc acttggtcta aaccgatacc gatgatgtcg
    63181 gcagctttta gccatgaata gtgtttagtt tgtggaacca accattttcc taatttttct
    63241 aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt cgattttttc tgggacgctg
    63301 tctaacagat tcatgatttc ttctgttgac aagttcatta attcccaatc gctttttcca
    63361 gcaactagtt caggtgtcac ttctttcatt gcaagcggca atgatttgat gttgcgggca
    63421 taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc ccagccatct
    63481 ttatagctca ttaattttgc aaattccatt cctacttctt cttccatttg gcttgttgca
    63541 ggagaagatt cataggcaac gttattacca ttccatagca tagcaaaatt gtacgctagt
    63601 aaagatggca tcaatgtttc agagttcata tgaccccagt aacgtccggc attatgccaa
    63661 ggaacggaat gggtacgcat ccgcgctgaa atttctgata gaacatcctt tgttttattc
    63721 acagtgtgtt caaaactagc actgcttttt tcttctggcg taataatagg catgtcttga
    63781 ggcatgtagt tttgacgcca gcctaaatgt tcatccacca attcattcaa caaagcttta
    63841 tagatttggc cgttctcagc tttgtcccca atgaataggg catttaggtt tagatctttc
    63901 gacaatgatt cactcatagt gaaaccctcc taaaaaaata tttaaatttt gtcgagccct
    63961 acattacacc tgttcttcta ttctgtaaac taagttttgg cctgattttt aatcatattt
    64021 taatatgtat caattatccg aattgttcac acttttgaaa taattaaaaa aaacgaggga
    64081 atttacaaat gtaaactccc tcgcaaagat tgtattaacg acaaccagcg ttctatttta
    64141 ggatgggttt caaaattgga atgttgtaat agtatttccc tttttcaagg taaaaactta
    64201 ctaaattcta atcaattact ttggccaaaa agtaattttt cttacctttt cgaataacca
    64261 caaacttacc atcgaattca tcggatggat ttatgacaaa atttaaatcg gtaacgcggt
    64321 caccgttaat acttatagct ccgtttgaaa catcttcacg tgcttgacgt ttagatggtt
    64381 caatttttgt ggaaacaagc agttccacga tattttcagg tgtgcttgaa atttcaacgt
    64441 tcggcatttt accgaatcct tgcgcgattt cttccgcatt tagttcttta atatttccag
    64501 agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta tgcacaaatc
    64561 ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct gtctctactt
    64621 ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt aaagaatttc aagtatttca
    64681 ctacatcgcg atcatcttga ttcaaccaga attggtagaa ctcaaatggt gaagttttct
    64741 tcggatcaag ccaaacggca ccgcctgcag tttttccgaa tttcgttccg tcagccttca
    64801 gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat tttcggatta
    64861 ggtctaatcc agctgtgata tttccccatt ggtctgctcc accgatttgc aattgcacat
    64921 cgtatgtttt atgcagatgc aaaaagtcga tagattgcag gatttggtaa gtaaactctg
    64981 taaatgagat tcctacttcc aaacggctgg caacgatatc ttttgctaac atcgtgttga
    65041 tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa agagattttt gacaaccagt
    65101 cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa caattttctc atttgattgg
    65161 aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg caacacacgt tctgttttgc
    65221 ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat cagaatatat ggatgatggc
    65281 ctgccaattg gaatcttttc atcatcataa atggaatcaa atgtccgata tgcatcgaat
    65341 caccagttgg atcaacgccg caataaagtg aaatgctctt ttcttcaacg agctcgcgaa
    65401 gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca agttaattca tcaataatat
    65461 tcattttact gacctccgtg tagttttttt atgaaagcag gaacctgcta gcatatgtaa
    65521 ttaattattt cttcaggata ggataggaaa taaaaaaagt cccttggcat ataagccaag
    65581 ggacgaaatc tcgcggtacc acccaatttg tatgtctaga tatccaacat acctctcgaa
    65641 catttaacgc tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa ttcgctttaa
    65701 agcatgtact ggtttgcagc gaccaccagc tttctgaaac attggcttta acaactactt
    65761 cgtttcgatc ttcgtcttgt tattattaat atgtgtctat catagagcgc aactttttct
    65821 ttgtcaacag aaaaaggata ttttttttaa attcctcatt ttttcttttt tgaatccgct
    65881 ctaattccga acattttatt ttcaattatt gttaaaagaa ataacttttt ctgtattttt
    65941 tctgcaattt atcgagaagt cactttactt tgcctcttat tctttcttcg ttacaataag
    66001 gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa aaaagcaaaa
    66061 aattatttta ggagccactt ctgctttaac tatcgttatt ttaattctaa gtttgttttt
    66121 tgtcatccga tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa taacagaaaa
    66181 agcaaaagaa ccgacagatg ataagcaaca actagttact caagcaaaaa atctggctca
    66241 aagctacgat tatgaccaag cgattgcgtt attggaaaaa gatagtaaga acggatctac
    66301 tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa gaacaactga tcgactggtc
    66361 tgatcctact cagatctctc atgtcttttt ccacagcttg atcgtggacc cgcaaaaagc
    66421 cttccactct caacaggcac aaggatacaa agattatatg gtgactgttg aagaattcaa
    66481 ccgttcgatc gaacaacttt atcaaaatga ttttgtttta gttaatttga atgatttgat
    66541 ccaaaaagac gaacaaggaa atctttcctt taacgggctt gcgctacctg aaggaaaaaa
    66601 accattggtt ctttcacaag atgatgtcag ctattatgaa tatatggata attccggttt
    66661 tcctagccga ttggtcgtat caaataacaa tgaaatcaaa aatgtctata aagagaaaga
    66721 taaagaagaa atcggtgact ttgacatggt tcctctgatc gatgcctttg tcaagaaaca
    66781 tcctgatttt tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt acaacggagt
    66841 tttaggttat cgaacatcaa aatctcaata tggagacaat gaaaaaacaa aaaaagagat
    66901 taaagaagct aaaaaagtgg cggaacagtt gaagaaagac ggctggagct ttgcttccca
    66961 tacatggggt cacttgaata tgactcaagc ttcactagaa gatgtaaaaa aagacaatga
    67021 attgtggcag caagaagttg cacctatcct aggaaaaaca aatattctga tttatccatt
    67081 tggtgcagac atcagcgact ggcagcctta ttcagcagaa aatcaaaaat ttgcttactt
    67141 gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct tctacaccag cttggggaca
    67201 attaggtacg gatttctatc gaaatgctcg gatcaatatc gatggcattc gttttcaatc
    67261 tgatttagac ggaaaaaatc ctattcttga tccattcatc aatgtccatg atgtctatga
    67321 caaagaagca cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa agccgaactg
    67381 acattttatc gtcagtttcg gcttttttat gctttatttc ttcttcggtg cgattccctt
    67441 gatcaggact tctgtccatt ctgctgtata tggaagcagt tttgcgtata tctccggctt
    67501 agaaattcct tctaccattg cttgaaaata gaggatcaag aactcatccg aatatttcaa
    67561 atccactttt ccttctttcc gccctcgatg gaaaagatca agcatgatac tgaaactttc
    67621 ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt tctttttttg tatagtatgt
    67681 catcaaatcg atataaaatt gtttgttcac tcgattgaga taacggattt tgttttcact
    67741 catggcaatc agtgtttctt caaatggaag attttccgca atcaacgctt tcgcttcttg
    67801 ccctaaatca ttcataaatg caaaaaatac ttcataaatc agcccttcct tgctgccaaa
    67861 atatttaaaa atcgtcgctt tacttacctg agcgttctca gctaccttat ccattgtcac
    67921 tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa tctctttctt
    67981 tttctcttgt gctctctttt caaaaccgtt catttgtaga gcctcccgta attttaaact
    68041 tttataatat atatagttca aatttattct gaggtttatt ataactatct attgagaaag
    68101 tatcaagcta gcaattcctt gtttatgaat tcattgacag aaatttataa aaagaatatt
    68161 atgtactaaa ataataaaaa aagttttata aaaaggtata aaaccttttt attaaactat
    68221 ttaatcttgt ttagtatata tttttagaaa aaggagtggg gtttttatgg cagaaatcgt
    68281 caaagtcaca catttgaaaa agagctttgg tcgtttcgaa gctttaaaag atgtctcttt
    68341 caccgtacat tcaggtgaag tcgttggatt tatcggacca aatggttctg gaaaatccac
    68401 gacgattcgg acacttttag ggattataaa aaaagacggc ggaacggcaa cgatttttgg
    68461 gcaagatgta tgggaagata gtttatctat ccatgaacgt ctttcttatg ttccaggcga
    68521 tatctctttg tggggaaatt tgactggagg agcgatcatc gatctcttca tgaagcttca
    68581 tggaaaaggg agtgctcaaa aaaaagatga actgattcaa cgtttctctc ttgatcctaa
    68641 gaaaaaagca aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga ttgctgcttt
    68701 ggctgttgat tcggatctct atttatttga tgagcctact tctggtttag atcctttgat
    68761 ggaaacgatt ttccaagaag aagttgaaaa attgaaagaa cagggcaaat ccatcctact
    68821 ttcttctcat attctcagtg aagtcgagcg actagcggat aaagtcgtga tcatacgcga
    68881 aggaacactc gatgaagaag gaacactcga tgaattgcgt catttgactc gttcgaccat
    68941 cactatagaa acagaagcag agaatagtca cttagcacaa ttgaacggtg tccatgactt
    69001 tgtgcaaaaa ggtcacctcg ctcaatttgc tgctgataac gaagctttcg atgtgatttt
    69061 agctgaagcg tccaaattag gtgtgaaaaa atttgaatct attccaccga ctcttgagga
    69121 tttattcatg agacattatg aaggatgagg aggaagcgaa atgactgata aattttcacg
    69181 ctggtccacc cttttttctc aaaaccttag ccgtgactgg aaaaagatcc tgatatggct
    69241 agtcggtgtc actgcttttt ctggtggttt tgtccctgcg tttgaagaat tgacttccgg
    69301 acaaggatta gctggattat acgaaacctt gcaaaacccg gcaatgattg caatggtcgg
    69361 accaactcca gcttcgaatg ctgctgagta tactttagga gcgatgtatg caaatgaaat
    69421 gttgcttttt tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg tgagccaaac
    69481 gagaaaaaaa gaagaacttg gtctgattga acttattcgt tcctttcgag tcggacgtca
    69541 agcaaactct ttagctgtca tcgctgaaag tattctgatc aatttgcttt tagcaatatc
    69601 gatcagctgt acgatgattt ttttccaagc ggattcgatc actgcagaag gtgcttggct
    69661 ttttgggcta tctgtaggta gtatggggat tttggggagt gccgttgcac tgtttgctgc
    69721 acaactgatg ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt taggtggact
    69781 ttaccttcta cgagcaataa cagatgtagc tgattcctcc ctttcgatgt ggaatccttt
    69841 aggctggtct tatttgacct atccatttac aactaacaac tggattcctt tgctttattt
    69901 gtttttgttc agtctaatct tgataatcag ttcatttgtt ttagaggaac atcgagattt
    69961 gaacaccggc tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt ctcttttatc
    70021 cttgcgtggt ttattattct atctaaataa agggatgaca atcagttggc ttatcacttt
    70081 tggtttgatg ggggcggcat acggctctat ctacgggaat atgcaacagt ttttagaaag
    70141 taacgactta atgaaacaga tgtttatcca atctggcgtc tcaattgaaa aaaactttac
    70201 aagcacgatc atggtagtct tgatttgtct agctgctatt ttaccaattg ctagcatcaa
    70261 tcgtttattt acagaagaaa cccacggacg tatgaatcag ctgttcgcaa caaaaatttc
    70321 taggaaccaa ctttattgga ccaatatgtt actagctatt ctatttgcta tattggcaat
    70381 cctgattagt tctggtgcac tagggctttc tgctttggct tcgatgaagg atgcgaaaga
    70441 catggatcta ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg ttttagtgtt
    70501 tatcggaatt agtggctttc tattcggatg gcttcctcga atcggtaaac ttgcttatat
    70561 ctacttagct tactcctttg cactcaatta ctttggcgga attttggatt taccagaatg
    70621 gttttccaaa acagctgtcc ctagctgggt tccacggatg ccaaacgatt cttttgatct
    70681 ttccgtgttc cttacgttat catgtatcag ccttgcactg ttcgtccttg gttctattgg
    70741 ttataggcga agagacttac tagaaggata ataaaaaagc atgtacaatt tcatgctagc
    70801 ctccaaatct gggtcaaaaa tccaacattt ggaggtcatt tttttattta caagtcttct
    70861 aactgctctt tatcaatgat ccagacttct cgattctttc tttttacata accattctcc
    70921 tctaataatt taaattttct agataaagtt tcgggggtcg taccgataaa tgcagcaagt
    70981 tctttcattt tcattggcag gaaaaagtga tccttccctg tcgctttagc taagtcaagc
    71041 agatagacag cgactcgttc ttctactttt tccatcatca aaaaagttgt ctgctgctgt
    71101 acttttgtta atttctcaac gttgattgct aaaagtttca gactaagggc gggataatgc
    71161 agtaagagtt ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt ttctagtgct
    71221 tcgccaaata aagactcatt tggaacgcct agcaactggc tctctccctc atatccgccc
    71281 ggctctatta ctcgtaaaag ctgttccttt ccgctagcag ataattgata cactttcata
    71341 ctgccggagg ccacgatcac taattgaggt tctccattcg gcgcaagaat ctgttctcct
    71401 ttttccacat agtgatgttt gacaagtgcg tgtacttttt cttgatcttg ctgtactaag
    71461 tgattgaaca aaggaacaaa tgaaacacat aaatgatcat gactcataat ctctcctcct
    71521 tctaaacagc tatttctaat aaagaaacat aacttccctt attccaactg ataattttct
    71581 tttatcatag cataaacgtc aagcgaacca gataccagca aatagagtgg atttcttttt
    71641 gtcccttgct ttattttcct tcttttgaga ccttaagtaa ttgattccta tcaatttttt
    71701 aaatatttac tttttgaata atcaaaaaca aaccttcact cgcaagccga gagtaaggtt
    71761 tgtttttcgc attgcttatt tcgtgttgtc tattgaaatt tttccgcacc tagcttttga
    71821 tatagtggtg ctagttcttg taaggtttca gcggttattt gctcaacgtg ttcgatttct
    71881 tgcggataga agaatagttt acccatttga aaggttcggt ctctcatcgc ttttttcaac
    71941 tcttcttgtt tctttgtatc ggacaaatag tcagacaaag ctaagtggtc gtcaagttca
    72001 tgttctggtt gcggccatat gtaataatcc ttgatatcga ctgttttgct ccactctttc
    72061 agtatcgcaa gcaactggtt atacgctgca atgctaccat atcgttcttc gcttttataa
    72121 tgttggtaca tcaaatagat ttggtattgt ttttgattca acaaaactgc tagacaggca
    72181 ttctcgtctt gacgagattc gcttcggtac gcgctccaaa aatgactgcg taaattccag
    72241 ccattcgtcc agctttcgat tttaggtttg ctcatgccgt acatatcagg caaaagtgca
    72301 gctgtctgga gctgaatctc tttccatttt tgccagtgtt ctttatattc tttcttgatt
    72361 tgttcaatct tatctgttgg atggctctct ttgagctgtt tgaatgcgta gattggtcga
    72421 tcaaacaact gataatctga ttttccaagt tccattgttt cgcctctttc cgttatcttt
    72481 tgtattctac ctgaattttg cgatatggac aaatagaggt tgtgaaaaag ctgttctgca
    72541 tcaagtcata agaacagcca aacaaaaaac tgcgatgagc atcaccgcag ttttattgaa
    72601 cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta gaagcagctc
    72661 ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct tttttagcta
    72721 ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa ttccctgcct
    72781 tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca gcttctgcct
    72841 ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat ttttcttgag
    72901 aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca gatccgtata
    72961 acggaccaga agccccaccg actttcgata tcatcgccat agagaatact ttgaatgtat
    73021 cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa acccctcttc
    73081 ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg agatattgtt
    73141 tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct tgaacggtta
    73201 gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc tgtacattgc
    73261 tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga gaaagacctt
    73321 gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg acgcctcttt
    73381 cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa ggcgtcccgc
    73441 ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca tctaaaatct
    73501 tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga cgatagcctg
    73561 gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg aatcctggtt
    73621 tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta gttgcctgta
    73681 cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt gctgcatcgc
    73741 ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca gtatacgtac
    73801 tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata tcgtccatag
    73861 cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc ttaacgatca
    73921 gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga tctggagttg
    73981 gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct acaaacccgg
    74041 catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt tccgctttct
    74101 gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga tagcttttta
    74161 ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt tttttcaatt
    74221 tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg agtatcatct
    74281 gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc atccaccaga
    74341 tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga gttcaatacc
    74401 gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc attgactgct
    74461 tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag agaaaaaatt
    74521 tgcacctctt cggatttttg catctgctca atcatttctt taataccatc tgttactttt
    74581 tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc ttttacatag
    74641 ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat aaatttctaa
    74701 ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta aaagagagga
    74761 gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta gtagcttata
    74821 aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac ttggaggcgg
    74881 ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta atgggtgcaa
    74941 gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct gatgcgatca
    75001 ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg atggtcacat
    75061 gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat aactaaagag
    75121 agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc gaggtactct
    75181 accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc tgctgcgact
    75241 aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt tgtttttggc
    75301 aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc tgtcgtgctt
    75361 ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg ttggaaacta
    75421 gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc ttcgctgatc
    75481 tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact tagctttcca
    75541 gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg ttggatcttc
    75601 ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag ctttttctca
    75661 agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg atcgattgct
    75721 gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag ttcttttctt
    75781 ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact atttattgat
    75841 gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt ggaagtcatt
    75901 tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga aggttctgtt
    75961 tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat actggaaaca
    76021 gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat ccttactact
    76081 ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac acaacaggct
    76141 gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt ttccatgtcc
    76201 cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg atcgttatcg
    76261 cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat ttcaattttc
    76321 ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg accatttcaa
    76381 agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag tttccttgat
    76441 atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag aatattgacc
    76501 agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt ttaaaaggat
    76561 ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt gcccaacgtt
    76621 ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag ataatctcag
    76681 gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg atgtcttctg
    76741 tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga tacatcgtat
    76801 ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt ttaatgatgc
    76861 ccacgatact tgagaattca accgtttgag attttgcaat caatttggta cgattttgga
    76921 catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg aaataattca
    76981 catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt aaaatcaatt
    77041 tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta tttttttcga
    77101 tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt tctggtgttt
    77161 ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc aatttcgtca
    77221 tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc accatgatcg
    77281 tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta tgtttgtata
    77341 aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc ggataagaaa
    77401 cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct gtatgggtga
    77461 ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga atgtcgtatc
    77521 tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt cttttgttcc
    77581 aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc
    77641 atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc tagtcaatac
    77701 aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta agaaatacag
    77761 cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt
    77821 taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta aaagagccac
    77881 taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat ctttcaccaa
    77941 aattgatgca acttcttctc ttgttacttt tttcat
//
LOCUS       DASJUF010000003        76958 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJUF010000003 DASJUF010000000
VERSION     DASJUF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.003"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..332)
                     /locus_tag="U4B62_000144"
     CDS             complement(<1..332)
                     /locus_tag="U4B62_000144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140335.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(346..603)
                     /locus_tag="U4B62_000145"
     CDS             complement(346..603)
                     /locus_tag="U4B62_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140336.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(798..1340)
                     /locus_tag="U4B62_000146"
     CDS             complement(798..1340)
                     /locus_tag="U4B62_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140337.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1361..2452)
                     /locus_tag="U4B62_000147"
     CDS             complement(1361..2452)
                     /locus_tag="U4B62_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2140338.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2460..4670)
                     /locus_tag="U4B62_000148"
     CDS             complement(2460..4670)
                     /locus_tag="U4B62_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140339.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4686..7214)
                     /locus_tag="U4B62_000149"
     CDS             complement(4686..7214)
                     /locus_tag="U4B62_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2140340.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7266..7676)
                     /locus_tag="U4B62_000150"
     CDS             complement(7266..7676)
                     /locus_tag="U4B62_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140341.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7689..7907)
                     /locus_tag="U4B62_000151"
     CDS             complement(7689..7907)
                     /locus_tag="U4B62_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140342.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7904..8896)
                     /locus_tag="U4B62_000152"
     CDS             complement(7904..8896)
                     /locus_tag="U4B62_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2140343.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8975..9886)
                     /locus_tag="U4B62_000153"
     CDS             complement(8975..9886)
                     /locus_tag="U4B62_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2140344.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9905..10249)
                     /locus_tag="U4B62_000154"
     CDS             complement(9905..10249)
                     /locus_tag="U4B62_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140345.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10269..10825)
                     /locus_tag="U4B62_000155"
                     /pseudo
     CDS             complement(10269..10825)
                     /locus_tag="U4B62_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10906..11406)
                     /locus_tag="U4B62_000156"
     CDS             complement(10906..11406)
                     /locus_tag="U4B62_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140346.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQVGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11407..11952)
                     /locus_tag="U4B62_000157"
     CDS             complement(11407..11952)
                     /locus_tag="U4B62_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140347.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11949..13205)
                     /locus_tag="U4B62_000158"
     CDS             complement(11949..13205)
                     /locus_tag="U4B62_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2140348.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13566..13877)
                     /locus_tag="U4B62_000159"
     CDS             complement(13566..13877)
                     /locus_tag="U4B62_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140349.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13900..15210)
                     /locus_tag="U4B62_000160"
     CDS             complement(13900..15210)
                     /locus_tag="U4B62_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN2140350.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15824..16276)
                     /locus_tag="U4B62_000161"
     CDS             complement(15824..16276)
                     /locus_tag="U4B62_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140351.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16278..16643)
                     /locus_tag="U4B62_000162"
     CDS             complement(16278..16643)
                     /locus_tag="U4B62_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140352.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16742..21025)
                     /locus_tag="U4B62_000163"
     CDS             complement(16742..21025)
                     /locus_tag="U4B62_000163"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEN2140353.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21172..21411)
                     /locus_tag="U4B62_000164"
     CDS             complement(21172..21411)
                     /locus_tag="U4B62_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140354.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21501..21773)
                     /locus_tag="U4B62_000165"
     CDS             complement(21501..21773)
                     /locus_tag="U4B62_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140355.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21856..22107)
                     /locus_tag="U4B62_000166"
     CDS             complement(21856..22107)
                     /locus_tag="U4B62_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140356.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22270..23229
                     /locus_tag="U4B62_000167"
     CDS             22270..23229
                     /locus_tag="U4B62_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2140357.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            23399..24079
                     /locus_tag="U4B62_000168"
     CDS             23399..24079
                     /locus_tag="U4B62_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2140358.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            24169..25203
                     /locus_tag="U4B62_000169"
     CDS             24169..25203
                     /locus_tag="U4B62_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2140359.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            25217..25552
                     /locus_tag="U4B62_000170"
     CDS             25217..25552
                     /locus_tag="U4B62_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140360.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            25536..28016
                     /locus_tag="U4B62_000171"
     CDS             25536..28016
                     /locus_tag="U4B62_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140361.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            28072..29631
                     /locus_tag="U4B62_000172"
     CDS             28072..29631
                     /locus_tag="U4B62_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2140362.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            29628..30992
                     /locus_tag="U4B62_000173"
     CDS             29628..30992
                     /locus_tag="U4B62_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2140363.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            31163..32233
                     /locus_tag="U4B62_000174"
     CDS             31163..32233
                     /locus_tag="U4B62_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140364.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            32248..32916
                     /locus_tag="U4B62_000175"
     CDS             32248..32916
                     /locus_tag="U4B62_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2140365.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            32936..33514
                     /locus_tag="U4B62_000176"
     CDS             32936..33514
                     /locus_tag="U4B62_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2140366.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            33915..34826
                     /locus_tag="U4B62_000177"
     CDS             33915..34826
                     /locus_tag="U4B62_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2140367.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(35204..36121)
                     /locus_tag="U4B62_000178"
     CDS             complement(35204..36121)
                     /locus_tag="U4B62_000178"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN2140368.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            36323..36760
                     /gene="zapA"
                     /locus_tag="U4B62_000179"
     CDS             36323..36760
                     /gene="zapA"
                     /locus_tag="U4B62_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN2140369.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            36818..37366
                     /locus_tag="U4B62_000180"
     CDS             36818..37366
                     /locus_tag="U4B62_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN2140370.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            37481..39841
                     /locus_tag="U4B62_000181"
     CDS             37481..39841
                     /locus_tag="U4B62_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN2140371.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            39928..40242
                     /gene="trxA"
                     /locus_tag="U4B62_000182"
     CDS             39928..40242
                     /gene="trxA"
                     /locus_tag="U4B62_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN2140372.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            40424..42202
                     /gene="uvrC"
                     /locus_tag="U4B62_000183"
     CDS             40424..42202
                     /gene="uvrC"
                     /locus_tag="U4B62_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN2140373.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            42347..43246
                     /locus_tag="U4B62_000184"
     CDS             42347..43246
                     /locus_tag="U4B62_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2140374.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            43239..44501
                     /locus_tag="U4B62_000185"
     CDS             43239..44501
                     /locus_tag="U4B62_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140375.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            44662..46560
                     /locus_tag="U4B62_000186"
     CDS             44662..46560
                     /locus_tag="U4B62_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN2140376.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(46890..47609)
                     /locus_tag="U4B62_000187"
     CDS             complement(46890..47609)
                     /locus_tag="U4B62_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN2140377.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            47946..48278
                     /locus_tag="U4B62_000188"
     CDS             47946..48278
                     /locus_tag="U4B62_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140378.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            48265..50268
                     /locus_tag="U4B62_000189"
     CDS             48265..50268
                     /locus_tag="U4B62_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN2140379.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            50287..50757
                     /locus_tag="U4B62_000190"
     CDS             50287..50757
                     /locus_tag="U4B62_000190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN2140380.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            50778..51362
                     /locus_tag="U4B62_000191"
     CDS             50778..51362
                     /locus_tag="U4B62_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN2140381.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            51377..52363
                     /locus_tag="U4B62_000192"
     CDS             51377..52363
                     /locus_tag="U4B62_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN2140382.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            52353..52664
                     /locus_tag="U4B62_000193"
     CDS             52353..52664
                     /locus_tag="U4B62_000193"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN2140383.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            52686..54467
                     /locus_tag="U4B62_000194"
     CDS             52686..54467
                     /locus_tag="U4B62_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN2140384.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            54460..55836
                     /locus_tag="U4B62_000195"
     CDS             54460..55836
                     /locus_tag="U4B62_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN2140385.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            55843..56478
                     /locus_tag="U4B62_000196"
     CDS             55843..56478
                     /locus_tag="U4B62_000196"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN2140386.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            56508..57863
                     /locus_tag="U4B62_000197"
     CDS             56508..57863
                     /locus_tag="U4B62_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN2140387.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(58055..59449)
                     /locus_tag="U4B62_000198"
     CDS             complement(58055..59449)
                     /locus_tag="U4B62_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2140388.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            59541..60512
                     /locus_tag="U4B62_000199"
     CDS             59541..60512
                     /locus_tag="U4B62_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN2140389.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            60509..61291
                     /locus_tag="U4B62_000200"
     CDS             60509..61291
                     /locus_tag="U4B62_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN2140390.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(61460..61831)
                     /locus_tag="U4B62_000201"
     CDS             complement(61460..61831)
                     /locus_tag="U4B62_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2140391.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            61965..62408
                     /locus_tag="U4B62_000202"
     CDS             61965..62408
                     /locus_tag="U4B62_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN2140392.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(62451..63260)
                     /gene="yidA"
                     /locus_tag="U4B62_000203"
     CDS             complement(62451..63260)
                     /gene="yidA"
                     /locus_tag="U4B62_000203"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN2140393.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(63279..64646)
                     /locus_tag="U4B62_000204"
     CDS             complement(63279..64646)
                     /locus_tag="U4B62_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2140394.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(64630..65460)
                     /locus_tag="U4B62_000205"
     CDS             complement(64630..65460)
                     /locus_tag="U4B62_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN2140395.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            65623..66060
                     /locus_tag="U4B62_000206"
     CDS             65623..66060
                     /locus_tag="U4B62_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN2140396.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            66136..66756
                     /locus_tag="U4B62_000207"
     CDS             66136..66756
                     /locus_tag="U4B62_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN2140397.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            66945..67451
                     /locus_tag="U4B62_000208"
     CDS             66945..67451
                     /locus_tag="U4B62_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN2140398.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            67468..68367
                     /locus_tag="U4B62_000209"
     CDS             67468..68367
                     /locus_tag="U4B62_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN2140399.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            68454..69338
                     /locus_tag="U4B62_000210"
     CDS             68454..69338
                     /locus_tag="U4B62_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2140400.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(69530..69601)
                     /locus_tag="U4B62_000211"
     tRNA            complement(69530..69601)
                     /locus_tag="U4B62_000211"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69566..69568),aa:Arg,seq:ccg)
     gene            69661..70995
                     /gene="rpoN"
                     /locus_tag="U4B62_000212"
     CDS             69661..70995
                     /gene="rpoN"
                     /locus_tag="U4B62_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN2140401.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            71144..72235
                     /locus_tag="U4B62_000213"
     CDS             71144..72235
                     /locus_tag="U4B62_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN2140402.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            72276..73277
                     /gene="gap"
                     /locus_tag="U4B62_000214"
     CDS             72276..73277
                     /gene="gap"
                     /locus_tag="U4B62_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2140403.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            73387..74577
                     /locus_tag="U4B62_000215"
     CDS             73387..74577
                     /locus_tag="U4B62_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN2140404.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            74681..75436
                     /locus_tag="U4B62_000216"
     CDS             74681..75436
                     /locus_tag="U4B62_000216"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN2140405.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            75552..76850
                     /gene="eno"
                     /locus_tag="U4B62_000217"
     CDS             75552..76850
                     /gene="eno"
                     /locus_tag="U4B62_000217"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN2140406.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        24694 a        14351 c        14858 g        23055 t
ORIGIN      
        1 tatgaattca ttccccaaag gccttttaag attgatacag cagtccgttc acttctttta
       61 gacttgtcat acaagtcttt taaatgatcc catatcagac tagtttgtct atctagcttc
      121 aatccattaa acgctaatga acggttcatt ttttctatta ctttagataa agctacagaa
      181 atttttatac cttcacaacg cgcatcttgg atttcaataa tagtatcatc aattagtttt
      241 tgaatttctt gcctatcaac atattcaatc ttagaaagag aatcttttaa agctactaat
      301 gattcaacga tttctttttt ctcttcagac attttcttcc tccttctagt gacccgtaaa
      361 gccatgtaga gcagagtcca ttgaaccagt aattacacta tctacaacgc cattccaatt
      421 tactctacat tttccatttt tcagatttgt cggtccatac ggatagcctt tttgacattc
      481 aatgttcgga ttataccaga taggattata ccattcagcc gcagaagcag attcagtcga
      541 tacaaacaat cccacaccaa caaatccaat acacatcaca ctatagccaa ttagtttttt
      601 catctcgtat ccatctccta aaaaatagaa tagttcttat agttattcat aagacatatt
      661 ttttccgttt ttgttttatc ctttctgtcc tataacaact tttttcaagt aaagaatgac
      721 atagtttttc ttaaaaaaac agtctgtttt tttctatttc ttcggtgtat aataaatatg
      781 agtgttcatt ttttatttta atacgcactc cgtacataat aaatcttgat cgcatcaatc
      841 ttttgagctt cttgatctac tttcaattga agtaatacaa attgagtaac cttattcatt
      901 gtttcttctc caaataacaa atattgatcc ggattctcta tatcttgtgt cacctggtaa
      961 agtttcccag ttgttttcat ttgtacatgc gggtctacat caatactatt gtctttaata
     1021 caacgatctg tcatatagtc cctcacagct tgattgcgtt tataaacatc tgtgaaatta
     1081 ctccattttc tagcaaaatc ttttaagaaa ttttttgtca tttcatctat ttgcatttgt
     1141 tgcgctgtct cgtcttctaa tgaaccaatt tgactgttac tttcagtact ttgaagagtt
     1201 ccacttgttt cttttactgc ttcgattgtt tcttctgtcg ttctcaattg tgttttttgt
     1261 ttttcagaag aaaaccaatg acaaccagta agacctatca aacataacca actaaatact
     1321 aaaattatcc cttttttcac gtttctagtc actgcctttc ttatttcacc ttacgaacca
     1381 gactacgaat ttttgtttga ttgtacgtgc gttgataccg agccataatc cgtccttgct
     1441 cggcattctg ttccacacta gagaaatgtt gtccgccata tgaaacatct gttataattc
     1501 cggtgtgacc atagattcca ggagacaatg caccacctgc ttgccaattc accacatctc
     1561 ctgcttgtaa atcacttggt tttgggttca tgattacttg ccaaccatag gttgtccagt
     1621 tataatcttc ccctattaac atcgcaaaca tgtgtccaga acccattaac tgtgggccac
     1681 ctaattgaga tacataccat gccgtaccac cataacattc cccattattt acggatcgtc
     1741 caatgatccg atccaaaacc ttcgcattgc ctttactttt tcctgcaata agcgaagttt
     1801 tttttgttgg taggctattc caatgcttga tcgcaaaagt atcatattcc gttaagttat
     1861 aggacgttat gatactcatt gctcctcgat aatacggact cccttcggta atacctgctg
     1921 ccgcatagcc tccacgacct agaatcaata aacaacttcc aggatcttta ttgcccacac
     1981 attgattaaa gttgctttgg ctcgccacca taaagccata atagtcataa caagctttta
     2041 agttcggaaa cttcatatag aaaccacctt cagaaccacc aattccacgt gcagaacctt
     2101 gtgggtaggg acaaccagga aaccaggtaa tcccaaaaaa gttcgtatcg tttcttccag
     2161 attgtgactg tccataagct gattcatagc ctaattgaga aagaatcaca ctcggtaata
     2221 actgatactt tacagctgca ccaactgctg cttgcgcatg ttcatctgtg attggaccac
     2281 gtcctttaat ctctgaaaaa atcggtaatc cttcggtata ttccccatta aaagcgacag
     2341 ctagacctgt agcttctcct ccatgctcag aaagttctcc tgaaaaaaag aatggcaata
     2401 gaaaaaaggg aagaataaag aaaagaagac aacctagaca ccctttcttc atttcttcct
     2461 catcctttct tcgataatgg tttttggact gttcgtcctt ttggtaaagt aaaatcagtt
     2521 cctggttttg cgtccaaaat cggcagtgtt ttaggatcct tttctatttt ttccgattgc
     2581 tctgttggct tatctaaaga agaaaatgca ggttgtaacg attttggtgc ggaaatcgac
     2641 ggtttacttt cagacgaacg ttgccccggt tgttgcacag aacgatttcc tgatagagtg
     2701 gctaccgacg aattaggaac agagtattgg ttcaataact gctgcattgc tggataactc
     2761 ccatttttcg cctgattttt cacttggcga atctgttgtg aaagagaacc agcagctgtt
     2821 aattgtggca cgggtacttt tccagaaggc aacggaatcg attgaccaac aaaagcgata
     2881 cctttattta acaatggttt tctttgatta ttcccttgtt gcttactcaa ttgtgccaat
     2941 gattgtacct gttgttttgg tcttactgaa ttcatggacg aatttggaat caaaccattt
     3001 gaaagaccag aattccactc tgttttcgat ttggtagaag acatttcagg ctctacccca
     3061 tgataacgaa catgacgacc actataaggc aaggcttgat ttgaacgccc acctagttca
     3121 aagggattaa ccacttgcct cccttttacc gattcttgtg gtgtacgttt ttgttgaaac
     3181 ttttgaaatc catgtatcgc tgcagaagcg agtggattcg ttgctcgata cacattccct
     3241 gctaatcctg cacctttgat tgccaaattt tgtccagcag ttgatagttg accaggcagt
     3301 tgtttcattt gagacaaaga atgacttgct atgtttttcg tgttgtttat agtttgatct
     3361 gcagctccta accctagttt ctttaataaa tctttccgtt ttacaatgag taaaattgat
     3421 aggagtgctt tgacaaacat atttcctaaa aaaccaaaaa cagtattcat tggaattaaa
     3481 tcgtctacta tgttatagat caaaaatgcc accaaaataa tcacactgta aaaagatttt
     3541 tgaaacatca agcccaaaaa ggactttaag gtattcatga gcatataatc aaatactgga
     3601 acaaaagaag cgagtaatga aaaaggaatc acaagtaata aacacaacac caacatttga
     3661 aagataaaac gaatcatgcc aatcactaca agtggtaatc caatacttag attaagcaat
     3721 ggcaccatag aaccaacgac tgctttataa acagctttag ttggttgaat atagacacga
     3781 taagccttct ttttccctct atcttctcct gcagcagtgg tcacttttgt ctcaatatca
     3841 gataattctt cacttgacgc ttccttggct agaaattctt gtggttcaat ctctgcttga
     3901 tacaattctt ctaaatttgt tgtcccataa tttaacaata tatacggatc aacaacagcc
     3961 gctttataat aaagttggcg cactttttga atggcttcct tttgtgtcgt tggcactaaa
     4021 acaccattga catcttttgt cgccaattca tctagactat ttgtagcaga aaaaataaag
     4081 ccttctacct ccacggacaa atcatttaac cctttggtaa aagcttctcc tttactaggt
     4141 acgttactac catagccaaa ccaaaccatt gaaaaaatga caacgaagag aaatcgaata
     4201 aacaccattt ttgcgtaatg tttccctcta aatatacgaa gagtaaatag atataacaca
     4261 tacaagacga caatccccgc accaatagga tcgtatattt ttgaataaat agtatccgaa
     4321 aaagtgaaaa actggtgaat taactcgttc attgcagctc cttcatagag cttctcaatg
     4381 acaaaatcaa ggccttgcca aatcaattta cttaaactaa agaacatatt cgtcatcatt
     4441 tttagaaaat ctgtgccatt tgccccaaat aacattttgc ctaatgaaaa ttcactatca
     4501 gataaataag aagaaaaggt atcaatatga aatttcccaa tctcatagat cgattgatcc
     4561 acctgctttt ctgccgcata caccaatcca ccaacaaaaa gcagtccgaa tccaatcgtt
     4621 aaaaaaaaca aaacgcacca tttcttttcc ttatgcttta tataagccat tgtttcacct
     4681 catttctatt gcatgattaa cgtatcctgt tcttctactt gagagtgact tgttttttcc
     4741 gttgtttgta atgcagcggt catttcttca aacaaactgt gtacattgag ttttcctaca
     4801 cgtccatagg gatctaacat ccaacactgt cctttaatca tgtggctcat ttcagctaag
     4861 ttcgcttcat ttacttccaa acgtaaatga ttcaaaatct tttctcgctc actgtcttca
     4921 tcaaaagcaa agacgacacc aaaattccct ttttctttat ctgaacctaa gtcaccaata
     4981 aattgtgtat ctaacactaa ttgattcatc tgggaacgcc ccacacgtgc cattgctgca
     5041 ataatactac gtcctgcgtt cgaattatta aatacccatg cttctgtaaa ataaatcgct
     5101 gttttcttac tactatctcg ttttccaaac atttcacaaa aacgacctaa actaatcatc
     5161 aaacacaaag actttcgatc cgcatctgta tatagttctg gtcggacatt gtcatttggt
     5221 aatttcaaat cttggatctc taaaatcgtt gttttggcat taaagtctaa tcccgggttt
     5281 tctccataag aaaatcctaa acgtaaaatc gaatcttcta ccgtcaagcg taaaaactca
     5341 ccatattctc gaacctcttt ttgttccatt tgttctaaat agtcgaccac atgcatcaag
     5401 ccaacttgtt gtccggcttc tttttgaact aaaacttctt ggattccctt taataccgcc
     5461 ccttttaaaa ggtgattggt acctaacgga cttaactcat caatcatatc ctgggctact
     5521 tgtttcgcat ctactcggga taaacaaaca attggatcta atacaccata attacgggga
     5581 tttgtagcat ctaacgtgac ataactcatt ttttgcaaat gttctacaaa caaagggtaa
     5641 tgtttctgat aatacgggtc ttgaatgagt ttcgtaaacc atttgcgctt ttctcctttg
     5701 ggatccacaa ataaactttg tacattcata aacgaactat acaaaaagag caagccaact
     5761 aaaaaggtct tccctttacc ggtttttcca gttactgcta catgaggcgc atcaaacaac
     5821 gcacctttga ttccttccgc aatggctaga agattcaaaa agacaaaacg atttgacgca
     5881 taaattaatt cttctaatgg tgttttttct cctcgagaac caccatcccc taatgtatct
     5941 tgccgaccaa tataccagcc ggattccata cctaattctg atactgctga aaacaaccat
     6001 tcgccaactg cttctacagt tgttcggtgc ttccaatgta actgccgtcc caatggttgc
     6061 cctaataaac atttatagaa caattctcgt tgatcatttt ttgcacggtg gatatggata
     6121 atcgtatgaa gttgttgaat aaccgttgca ctattttgac gaacttcttc aaccgtttga
     6181 ccgtatacaa taaaacatgc gatccaatcg acgacaggtt gcccattatc tactcgttgc
     6241 tttaaatgtt ttactaatcc tcgaacagtt aataagcgtt cactaggttc ttcatcattt
     6301 tgaaccaatt ccttatgctc tgttttgaac cgttccgata gccaattcag ttttaaacca
     6361 atacctccct gtcctttcaa gcgtggatag tgaattttca ttcgcaattc tgctgggaaa
     6421 gaaagatcct gagcaaattg aaatagatca caatttctca tattttcagg tgtttcccca
     6481 ataggcaaca atgctaaata actactccct aactcatctt ctaattgaag aaggccttta
     6541 tgttccttcg gtatacgtac atacgttcct tgattgtctt tcagtacttc ccaattattt
     6601 tgttcttcta ctttgtgagc cattcctcga atatattgca gacgattgag atacaacagc
     6661 tcttttttta ctaagggacg aatagaaaag ctataaagta attgtgtcac ttcttttaca
     6721 taaggatcac tttcagaaaa caaagtttcc aaatcatcta cttctaatcc aaataagcgt
     6781 gctaaacttc ctgctaaatc taaccagatt agatttatta catcatgaaa actctccacc
     6841 gttgcttttt ctttgacttt gattcctaaa ataaatgaag gacgatagac ttgaccttct
     6901 cgttccaaca atgtcactgt ttcttttgca taatacgcac caattgcttg actatcggga
     6961 gaaaatccct ttcctaatgt gtcaaacttc gtccgtaaat tcattgttcg tggatacatc
     7021 accaactcca catcttcata atctgctaaa agcaacagta aacgagccat catttctttt
     7081 atttcctgtg tcttttttgg tgtgtttttt gaaattgttc gtggaaaaac atgatagtag
     7141 gcccagatac aatcctctcc atttaacaaa caatttttct tgaatgcttt tactggttct
     7201 tttacaagca ccaatgaatt tcacctccca ataaaaaagg aacaaccagt tgattgtccc
     7261 tttttctaat cctctaaata aacatactta ttcacaaaaa gtaattcttc caatgcattg
     7321 tttgatacct tgtcccagtt ataatacaca caatcaccat tcatcatacg aatcatataa
     7381 caaaaccaat cccataaata aaaatgcact ttttttccat caggttttag ttttgccagt
     7441 gctaacacac ataaataggg gagtcctata taaaaaacaa ctcctgctgg tggaaaaaaa
     7501 gaaatgaccc aaccaataaa tgaacgtaaa aataaatatt caaagattac gaccaacaaa
     7561 aaagttgctg cattgactaa tgcaactgga aatggaagac gccaattgct tgtaatctgt
     7621 tgtacgcgat aaggttccgc ataaacaatc gaataatcat aaggttccca atccacttca
     7681 ctatcctcct aatccataaa taatttttca atgattttac caaacgcatc aaatacacgt
     7741 gttggatcat ttaagaagaa ataaactgcg gcacctgctg cgatagatat taatgcattt
     7801 cctacttttg atttcataaa atttcgcaat cccagataaa caaccaccat tattaaaaaa
     7861 tagccacctt gcgttaatat aaaatctaca attcccttca gtgtcatttc cgttcttctc
     7921 ctttcctatc caaccaatta tgtgttaatt tttcaacata atactgattt gtttttttcg
     7981 taatgacaat cgtcatttgt tctttttgta aaagtgacgt atcaccatct cgaaagacga
     8041 ccgttgcttt tacaataaaa ggttttttcg tttctttata acgcaatact ttcatctcta
     8101 cttgatcaac tttagctgtt tgattgagtc cttcaggttc tttcatcatg tatgacatat
     8161 cttttgcatt gtttttcgca tacttatcta agaaatcttc cacaaatcct tcaacatctt
     8221 tcgcttccga cgcatcaacc gtttcgtata atgtgtcatc ataagtaatc attcgttgtt
     8281 tttctgtatt ctttggtagt gccgtaaaat aaggataagc aataatacga cagcccttgt
     8341 ttgaaataga aaagggaaga ttgagtactt gtgtgattgt cacacttgtt tctttcttac
     8401 tctctggatt catcgagcga atatcataag ttactttata actagcagtt ggaatggttt
     8461 caatttcttt taatccaata aacgaagcag cagttaattt tcgaacacct gaaacacttc
     8521 ctggttcttg aataaagttt tgtgcataaa actctttttt taatgtttct tgacgttgtt
     8581 gttcttgttc atgattatcg aatgtattca tatacaaagc aacaaaacga ttcataaagg
     8641 attcaatttc aggtgtgtac gttaattctg accgactttg tacttctttc ttttgtaatt
     8701 gttggatttc atctttcaaa aacgacactt gaaccttcgt tttcatggat tgaaaaaatg
     8761 ccaagccccc tgaaaaacaa agaaataaaa ccaaacacca aatccatttc actgcttttt
     8821 ttgaatggat tttcttttgc ttcggtactt tctctgtata aactttttct ttccgccgta
     8881 gttgtcgttt tttcataagc tactccttct agtatgtttt tactagtacg tacgattgac
     8941 aacgataagc ttgtttttct gaaaaatagt ccattcaatt accctcattt tctattcctt
     9001 ttctctttct tttatatcca acaaatccag caatacttgt caacactcct cctagaacag
     9061 aaagtgcaac atgtatttct tcaccagtct ttggtaagac ttttgttttt ccatctgctg
     9121 ttttgatttc aatattgcca cttggaagtt tctttacagg aatcgacaaa tcctttacca
     9181 aattcccttc actatcttgg attaatggaa taccatcttt tacaccaaca attgtttgat
     9241 cacctgctac ttttaatggg gtggttggtg ttggaatatg ggtcaattcc tctacattac
     9301 tagtgacaat cggtacactc attccttgag aagtattcgt cgtaatttca ccgcttgggg
     9361 ttaccgatac tgtgacttct tttgctggaa caataggttt attttctttt ggaattgctt
     9421 ctgatttttc aacaggttgt ttcggttctt ctttttgttc ttcactggta gacgacatcg
     9481 ttggctgttc cgtacttgac gaatcagtat tcttttcaga agaactggtt gttcctgatt
     9541 gttccggagt tgtgactata cttgaatccg ttgttgttga actatgcaca ccttcttctt
     9601 cgatcgaatc agtcggtaaa gttgattcag gaaactctgg ttctggtgtc actggctctt
     9661 tttcaggttt ctctgactgc aattgttcag gttctgctgg tttttccgtt gttcctccat
     9721 ttagctctgt ctcaacatct ggtaatcctt cccctggcaa tgtttctaca atttctgact
     9781 taggtgtttc tacagtttca tctgcataaa ccaatgaact ccctaattga aacgaaaata
     9841 atgaaattcc tagtgttgtt acgactgcta atgttgtttt tttcatgatt attcctccaa
     9901 ttttttatgc aattcgtgtt ttgttcatag acgccgcact aatattatct gaacgataaa
     9961 agaactcccg tccgttttct tctaacccaa catatggatt atttttgaat aattgaatca
    10021 atagttcact ggtgttgtat tttggatagt ttgcaaaaaa ataatactgt gcttgtcgcc
    10081 catccgaaaa ttcaacaagt aattcataac gataccgctt caatcctttt cgttgtttta
    10141 aaggatctct ataccaactt gtccatcgtt ccattcgtgg atagagatag acacgataca
    10201 catagaacaa tgccatacca cataccacca aaaataataa tcgccacata aaaattcccc
    10261 cttttcttct acctttcttg aatcatcgaa cattcatacc ctctatagag atttgaaaaa
    10321 tccatcatca attgttcata taaacgttgt tgcaaatagc gattgtttcg aatacataat
    10381 tcttcatagc catcaatcac ttgatcttct ttttcatagc atcttgtagc tctatgacgc
    10441 tcagcttgca tgacataacc aaactctgca ccatatcgtt ctactgttaa taactgttga
    10501 ctacgcaata atgcaatata acaatcacaa aaatgttcaa ccgacatttg atttttctca
    10561 cgtttgtgaa ataggtattc tttagaaagc acattattcg ttaaacaatc tgataaatag
    10621 cgataatctg gcggtcggtt attttcatct gtgaattttt cccataataa actgattttt
    10681 attcgttcag cggctagata gtcttgttcg cgtggataaa attcatcaat cgcacaaagt
    10741 tttttatcac ctgaataaac aaatccatat aatgcgaaac gaactcttcc ttttcaattg
    10801 gctctcctaa aaattccagt gacataccat cttctccttc ctaaaaaaaa taaaaaagca
    10861 gaaaccgatt tggcttctac ttttctttcc ttttatagct cttttttacg gaagaatccg
    10921 caaatgacat tttttcaaaa aatcaatcgg cacaagttca cgaaaacttg gagcaatttt
    10981 ggatagaatc acttccccaa cttgtaaaat acaatcataa aaatcatcag ttacttgata
    11041 cttacgcttc ttacaataat tgataaaata gtagcgatca ttttctattt ccattcgttg
    11101 ttctactaag cgcatacgaa catcatcata gaaataagcc acaaatcgcc cttcaagttc
    11161 atgtttttca atgggatact cttctggtaa caatctattt tttcgtcgtt tatacattgc
    11221 tggatacctt agaagaagtt catctaaacg tctgtcagta tatggtaact gacgaatcaa
    11281 cgattctaat cgttctttat acagatagaa taattggcgc aagcaatagt gtaccgcctc
    11341 tggattttct aatactgttg gatcacgtga ccaatacgca ccaacataaa tcataatttt
    11401 tttcatttag acaatcacac cagcaatact aatgaccgat ccacaatagg tagaacaagt
    11461 ggcatagcga ccaatttcct ctaattctat ctcaatctct tctaaagaaa gatatgtctg
    11521 ttcttctgtt ggctggcgac tcatttccca agtgacagtc accaatgaaa tttcttccat
    11581 aagaggtttt tcgctgctaa gtgctgccac acagttttca tatatcttca ttttatcgca
    11641 gcctttttta accgctaaaa tcgcaggtat tggcaatgaa aatcgataaa agcgaatgaa
    11701 ttctagcctt cctttccttc ggctaaattc caactcttcg agttcttgtt cgtatccctt
    11761 acgaacttct aataagcgtg caatttcttg ccagttctct ttttcctgat aacgtccagc
    11821 ttttaccgct tgttcaatat aaggaactcg aaaacgaaaa taagacaaat caaccatctt
    11881 ttcatttaat gcttcgattg cagcctttaa atccaacagg cgcagccatt ctgttgtcgt
    11941 atatgcattc atgcaatttt ccctcctgac gttcccatcc attctagata gttttgttgc
    12001 tcgtcattta gttcggcatt atcaatccct tcttgtacaa agtctcttcc taaaatacga
    12061 tctgcttctt gtaacaactt taatgtcgga gctacttgtt tcaaaaacca gcgttctgaa
    12121 cgatctaatg agaatttttc tggctttact gtaaaatcca attcttccat cgaacgaaat
    12181 aattttgccc atcgtttatc aacaatccgt gcatcacttt ccagtctatc ccaaacaata
    12241 agttttgcat taattaaacc aacgccaatc tctcctaaac gtttcggtga atcacctaaa
    12301 ataaatgctt ctactgcttg tgttgctttc tcattggcta accgtaactc atatcgatta
    12361 taaataccat atttcgttaa tgcttctaat tctgaaattt tttctcggtt cgcaatttca
    12421 aaacgtttct cataaaaatt aaaaaagaga ggactctttc gcgatccaaa atacaaagat
    12481 aatccttcac ttcgaaaata gccatcatct aaaaaacaac cgccaatatt ctggaatctc
    12541 ttaaagtcca tttctaataa acctgctttt tgtttttcta gtaattcaaa gagatcgaaa
    12601 tgttcttccg tttccaatcc ccaaagttca tctaacgcaa tatcgaatcg gctacattgc
    12661 acacgtgtat aaccagcttc caactttcct tgatttttcc aaatggaata tagccatgtt
    12721 cgccaatctg tttgttgttc acgcatgact tcctcatatt cccggcaacc ttgtcctgaa
    12781 aattgcacaa gtacattttc tgttacagtt cgttttgaat gccagaacac ccagatattc
    12841 ccacgaacag aaagacagtc aaaaagatac aagcccgttg gacgtgtttc aaattctgtc
    12901 agatccattc gtaatacacg ttgaatcact gttttgtagt ctgtcccaaa aaaggaaaga
    12961 cgcaaataat caatcattgc tcgaagatct attccgcttt tcatatacaa tcgaaagcgt
    13021 tccttgattc gttgttcaaa atcctctggg atttttcttt tcccttgttc catctttcta
    13081 atgtaatctt ttgagcagcc catcaattgc gcaaatcttg cttgtgatag cggcttttta
    13141 tctccactta tattatgaat tgcacttaac ttctttctgt agctttttag ctcaatcggt
    13201 gtcattttct cgctcctttt ataaaataac aaaatcgatt ttttttaacc ctttagaaac
    13261 accgatatat caactagtcc ccctcgtttt ttgggaaaag tagtacaact gctgacctta
    13321 gataatcact tgatacaaaa gggtttataa aacttttttc tatgtgttat gttatttctg
    13381 ccccccatat tatatatcgg ggggctttac acaaagaggc cgtgcgcaag cgcacgacct
    13441 ctttgtgagc tgcacacgct gctagcgagt gcgcattata ggcccatgct tcgcagcctt
    13501 aatgcgcacc gccaggcaga gtgcggctac cagactacgt ctagagccct cccgatagat
    13561 aataactaca aaattaaatg atctaatttt gttatttcac tatatttttc aggagaaaaa
    13621 acgcgataaa ttgacaaagt taaatcctct tctaaatcta ctcgataatg atacaatcga
    13681 gtcaatcgtt ctatatcttt tttcgttagc aatacttgat taataacttt tcctgtaaaa
    13741 acacgttctt tgcgcaaaaa gcggtaatca gttacttgtt caatacgatt caaccaatca
    13801 taaaacgttc tggaagaaag agaaggatta atcattcgta ttgctgtagc tactctataa
    13861 taacaactct ctgccattta acttcacctc cttagttgat tacaaaatat gcgcatcaga
    13921 aaaacttgtt ccttttggat cttttgctaa atacatttgg cgctctagtt gttcttgtaa
    13981 caactgatta tcatctccca tcgtttgatc ttgactatcc tcttttactg taaaatcata
    14041 gacaatcatt tcagtaaatt tctcaaattc tttgataaag tgataatctt tgtaaacaaa
    14101 tggcgcaaaa aactctacag gtacatcaaa tcctggaaaa gcatatccac gaccaggttg
    14161 atctttgttg taaaatggct tatttttaaa atcttctcca aaagtcatta aataaccaat
    14221 gggagacaat cgaccaacag aaatacggaa tgttaaatta tcccgaacac cacttggaaa
    14281 atattctgca tctggtcgtt gcattgctaa aataatgtac atccctgctt gacgaccctt
    14341 taatactaat ggctgaatca atttatcgac tctatttctc gcttccatat ccaacgaacc
    14401 ataaaaagct gcccattcat caaaaaagat aaaatgcggt ggtaacccat aataagcata
    14461 atttttccct ggttcatatt taggatcttt catggttttc atataggcat accgttgatt
    14521 catttctttt aatgcatttc gtaaacaaat aatcatttct tcatttattt tgtaaaacac
    14581 atgtcctttg aatacatcga cgtcacctaa tgctgctaaa tctgccgctt ttccatcgca
    14641 aatatctact gtcccaacac ttaaacaagc ttgaattaaa gaatacaata aataggtttt
    14701 cccaccaccg attgcaccag caatcaacat atttggtgat tgatcatata cccaatccaa
    14761 agttttcatt aaatgaatac gtccattttc tgctatacat tgatcaatag gaatccgacc
    14821 agaatctgaa aggaatacat aattgattcg ggctttttct cgttcttcac gaattaaatc
    14881 acgcaaaaat acttcagata acaaatgacc catttttaaa aatcgttcgt gaaaacgccc
    14941 accatctaaa ggaaaagaaa ccactaattc ttgtttcttt tgataaaaat aaacttttgg
    15001 taatctcatt ttttcccttg tttgtttctc actaacttgt ttctttgtat aaacaaaccg
    15061 attgtcaatt aacattcgaa caagtaactg acgccaataa acagaggcaa aatatccttc
    15121 ttttagtcgc gaccaatgca tcaaccctaa aattaaccca ctaaccagca acaaatacaa
    15181 cacaatactt attccaggaa taagatacat ttgccaattc gaaaacgtac ttaaacgtgg
    15241 ataaaatact ttccaactaa accaacctaa cagaatcaaa aatggaaaca aaaagaaacc
    15301 ataatagcaa aaccataaat aacgattgaa atagcgaatt cgccgaccac gatacttgaa
    15361 aaaatgagct ttcaattgtc acattcctcc tttctgtttt tacttttcct gtgctaaaaa
    15421 cttttgacaa aaagcaacta atacaggaat atcacattta gtaaaaaaac ataatcgaaa
    15481 aagatttaat cgatttttat aagggtgtgc acacatatta catattcgta ctatacgaat
    15541 ccaaatcact tcctgatgat cttgtttcca ctggtacggt aatgaaaaac gaacaagatc
    15601 acgtaaaaac atacactgcc caataaccgc acaaatatat gcatttgttt gtacaaaaat
    15661 cgttggattg tctccaaaat actcaatcaa ttgttggata ttttgataac aagcacaatc
    15721 tctcaaagta tttgattggt tttatctgaa ttcaaaatct ccccttcctt ctatcttaga
    15781 taaaaagaat aggaagactc cttcctattc tttcaaaaaa accttatttt ttctcttttt
    15841 ccttagaatc aacacttggt tgtttttgat ctaaaccttt ttcttgtgat tgtttcatat
    15901 taggattatt tgttggagct tctactttct ctaatccttt cgcataaatc actaaatctg
    15961 aatattcacg attgccatct tgtgctggaa tatataccac atgatattct ggatcaatta
    16021 atttcacagg atcacgtcgt ttaaaaccag tcaaatctac atctgctgga aatttaactc
    16081 gtcgtgcaat ctttggttca tctggatttc caaaaactaa ttgtgctcgc tgtgatcgaa
    16141 tttctcccgt ataatcgcct gtttcattat caaatacacg atcagactct tctaatgcgc
    16201 caaagcgtac ggctcctaaa caagtttcat ctaatgcttc tgtaatgtca taattatttg
    16261 gaaattttaa tcccatctta caaaacctcc ccaattttta caccagttaa aaagacacca
    16321 acatcttgat agccagttgt tcgacgctct gtttgtccca tatcatttgt ttcacgacta
    16381 ttcactcgtt ctttacgaaa aacaaaataa gcttcttcaa atgaaatacg cacctctttt
    16441 tctggtaatc gtaatctttt atcctcttca gctgtgagat aaacaaaaaa agcaggctgt
    16501 tcttctcgcg aacactctgc ttgtaattga acatatcgcc cagacacacc attaatttcc
    16561 tttggtggat taggttcccg tgaacccaat aaaaatactt gacctaaaaa ctctttcttc
    16621 atacgcaatg ctttttctgc cataaagttt ttcctcctct taaaaaccaa aataaattct
    16681 aaatatttct acattacaat caaacaagcg gttaaaaatc ctaaccgctt gttcctcatg
    16741 actaatcttt ggctttacgt actttataaa gtgttatacc taaagcaatg actaatacta
    16801 ctaatccaag aaccataagt atactacttg tttgttcacc tgtttttggc aacattggta
    16861 caacagtagc tggtttaacc cgttgttctg gtttcactgg ttgcggtggt tttggcgctt
    16921 ccacaatttg aaccgtttga tctttgtttg ttagatctac attgtgtttc gccacttctt
    16981 caaacgtttc tttctcatct tccgttttcg cttggagcca ttccgcaaaa ctcaagcctt
    17041 ttcctgcaag attgcgaccg tccactaaga attcaaataa gaattccatc atttctttat
    17101 cggccgcaaa gactttttcc gcatgagtgc cttcaaatgg tgtgccgtct ggtgttaagt
    17161 agccttgaac aattaagcga tagatttgac caatcgcaag accgtctact ttgacccatt
    17221 caagcacagt ttgatttggc aaggccaaga tctcttttcc gccattttta ttcgtcgctt
    17281 tcgtcgtaat ggttggatta ctgattttga tggtttggtc attgtcggta atatctgcgt
    17341 gagaagccaa taatacgcct tctctaaaca gatcttcaaa gaccacgact tctttgcctc
    17401 gcaacgcacg tgcgtctaga ataaagtcta gattgactgt accatccgtt gtttctgcca
    17461 caaacgttaa ggtacttgtg acttcttttc catctactag cactggtttt tccgttactt
    17521 tgtccattag aacaccgttg actgtgtatg tttttccaac gaccaaatcg tggtatttca
    17581 cagcgtctgt aatggtgact ttcgttaatg gattaacctc tttcaagcca gtgggtttat
    17641 gtgtagccgt tgtacgtaca cttgggttag ttactcgtac cgtttgccct ttgtcgttga
    17701 tgtctttatg gtttgctagt tcttcatcat ttctaaaaag actttcaaac acgacgattt
    17761 cacgaccatt tatacctgtc aaatcaaagg taaatggtac gatcaccgtt tcattttctg
    17821 ctttggcaat aaagccaaca ctcgtttcta ccgtatttcc atgcacaagc aagggttcac
    17881 cggttgcttt atccattaag atccctttga ccaagtatgc ttttcctgga agaatctcgg
    17941 taatttcaac ggtatcgttt aaggtaaaca atcctgttgg gtctgcgact gtcaatccgt
    18001 cttcaaagac agccgtggta tgaacggttg gattattcac tttcacactt tgggcttcat
    18061 cgttgatatc cgcatgtgct gcaacttcaa tgccatcacg gaaaagtttt tcaaaaacaa
    18121 cgatcgtttg acctttcaga acactagcat taaccacaaa ttcaaggtct tttgtgccat
    18181 tgactttgtc tgcaataaag gccaaggtac ttgtgatttc tttgccatct tgtaaaattg
    18241 gcgcattcgt tgccttgttc atcaatactc ctgaaatggt gtattctttt ccgacaaaca
    18301 agttggtata agaaacactg tctttaatga cgatcgtttc taatggatca accacttttt
    18361 caccatcggc tttatttgac gcttgtgttt ctagttttgg tggaatcaag taattacgta
    18421 cagaaaccac taacgtttcc ccatcatctg gcgcattttg tttgaatgca accgtgaatg
    18481 cttgtttatc tgtatttggt tggaaacttt ctaatggggc attttccaag aagtaatagt
    18541 catcggcaac tagacctttc actaaaattt ccccgttttg atcagtggta tattcaccaa
    18601 ttgcttgatt tttaccgtta tacaaggtaa agttcacacc tgcgagcggc gttttcttct
    18661 tgttccattc ttcatacttg aataatttta cacttttccg tgcttgacgg ttcgcaaaat
    18721 ggatcacttt gatcggttct gtatcattgg taatcgagaa cactaatggg ttttgattca
    18781 aaacgtaatt tacgggggct ttgatttctt ccaaacgata gcgatctttt ccataagtaa
    18841 gtgtaccgtt agttaggaaa aagccttttt cattggtttt aaagatatcc gttgtttctg
    18901 tatcatttgg aatctccatt gtgacataac gattggctaa agtatcaaaa atcttgaatt
    18961 ctgtattagc aagtggaatc acttttcctg tttcttgatc cactttcacc actttaagac
    19021 gttgttcaat tactttatct tctaaaacat aatggaaagt ttgcccattt tcagaaattg
    19081 tgacttcaag tggttcaaat agtttatacc cttcttttcc tttggtttca gaaagaatgt
    19141 acgtatcaaa gacaacggta tttttaccaa aagtagcata accatcattg tctgtcacaa
    19201 cggttttcac cactttgcca gttgtttttg acgtcaaagt gatttcaatg ccagctaatt
    19261 ttggtttgtt atctggattc gttgaatcga ctaaatcttt attagcaact ttcactaaat
    19321 caatggttcc tttgatcact tgatcttttg cggtcgcatt gacagttttt ccaggttcaa
    19381 caacaatttc aattgcttca tcagaaagca cataaccttt tggcgctttg atttctttta
    19441 aaacatattt gccaggatca agatctttaa tttctgcctg tactttgccg ttaatttttt
    19501 tcaaggtaat ttctttaatc tttttcccat ctgcttgata caaaccatag accgcatttt
    19561 ctaaagaagc tgcaccttgt gctttcacac cagtttccgc atcttcttta aacacacgaa
    19621 tgtttccttg ttttagaatt tgtaaattaa taacagacgc attaggttct gttggatgta
    19681 acacaccaat tgtttgagct cctggacgac gataggctaa tggcgcacct tgaactttaa
    19741 ttttattcaa ttgaattttt gctttatccg gtgcctcttt tgtgactgta atccatactt
    19801 cattgccgtg aatagataca ttgacacctt ttggtttttg aatgtctttc acatatttca
    19861 aaaaatcatt tttattcttt actttcaacg tttcgccaat ttttaccgta tgtgtttgat
    19921 tattaaaagt tgctttttca ccaaagttat taatcaaacg ttgaatttct gcttttcgac
    19981 gatcataatc aacacggata tttttgattg ggatttttgt gcccaaagct tcccaaatca
    20041 taaattgtgc cgcaatgtat tgttcatcgg atttgtcttt atttgtgaca tatcctaaat
    20101 aagagatcaa gcttgttttt ttccgtacat ttgaattttt caaatagttt ttcaaagcag
    20161 tagctgtata aacttgacct tctgcggctg agacaccagg ctcaatacaa aacgccactt
    20221 gtccattaat ctcaaaacga accatataat ttaaagtgaa gtttagattg ttcggactag
    20281 aagtaacacc aatattttcg ccccaatcaa ttttccgacc acctaaaaca gctgcattgg
    20341 ttgcacggcg aacacgaacc gaacgattgg ttggtactgt tactaacagt gtaggtgtac
    20401 ctgtttttgg aatgttctgc tcttttttct tttgattttc aatactagcc gtagattctt
    20461 tgtttggttt cgttggttga ttttgactag ctgcggttga agagattgtt ttttcaacgt
    20521 tttcctcatt ttttccttct ttgtcagcag tagccgctgg ttgttccact gtttcttcta
    20581 ttcgtttctt ttcttccgct tgtttctcag aagactctgg ctgcacagaa ggcgttgctt
    20641 cggcagcttt ttctccttct agagtggacg tttctgaagc aggggttgta attgaggcag
    20701 tttcttcaac tggttgtgaa gtaccaggac tattccctgg gtgttgtgtt tccccaatcg
    20761 tctgatcgag agtcgttttg gaattgaccg ttggcgcttc tgtcacaggg gcctgttcaa
    20821 cagtggcttc accagctggg accccggctt ctgctggttg gacttctgtc acttcagaag
    20881 cgacttgggc aatggcttcc atcgtaggtt gtatagccgt cgtttcttct gcatgtgctt
    20941 gagcagttcc caccaagaaa cagaattgag aaaaagccac caatgcgagt gctttcaacc
    21001 aattcatttt cgtttttgca tacacaagaa ttcctccttt tcctgagaat tttatataat
    21061 aaaacaaaac gccgttacca aaagtgataa cgacgtgaac tgtctcttct atttttatct
    21121 ggcgctctta tctgaacaca tttcatcaat gattttaccc tcccactata attacagaac
    21181 aaaaaaagaa cctgcttcgt ggaaatcaac tgtccatttc ccaattggaa taccagcttt
    21241 tttggcaact tcaccatcac caaatctcat gccataccaa ttactacctt catccttaat
    21301 ttctttccaa ccatatgaca tagcttcatc taatgaatca aaaatcatga cactattgcc
    21361 aattgttaca ggatctggct taagatcttc tggaagagaa atatcaatca taccataatg
    21421 atccgattcc tcagtattat tgtcattact agctactggt ttggttgttc tactggctta
    21481 ttttgataca acaagtactc ttattcttct tttacttctc cgttttcaat ttcccattca
    21541 aaccactccg caaagtcttg cttaaaacca tctactcgat acctattttg taaataggca
    21601 tcttctgacg tcgtaggatc taaataggta ggtttattct ctggacgagc atagacacct
    21661 acccatgttt cgtaatctcc ttcaaaataa ctatctacca aatagccttt ttttctaaga
    21721 atctcccgaa cttgattttt aattattgat acttgttcaa aaccatcact cattctatca
    21781 cctcaaataa ctctagattc tcataaatat tcccgacaat ttcaatccta tcaatgatct
    21841 ctaacaaatc gctacttatg cttccccatt cgtataagaa tttcccttcc gcaaaataaa
    21901 ttttccataa acttcttcat gatcgtccca accaatatca ctctcgaaaa attccacgtt
    21961 gttcttatct tttagctcag taaatgccat aagttcaaca tgttttccaa cctcgaaaca
    22021 atcacaccac ccatcaaccg ttacacgtgt gccttcaata cctaagaaac tttgtcctcg
    22081 tgtattaaac actaaaactt tccgcatttt ttgctcatta attaatcacg cgcgaaatct
    22141 aggaatcaca acttattcgc ttcttcttta taaatcgccg attgtaaaat taagctagac
    22201 aactaaaaaa agtcatttgt gctacactca aaatagttcc gacgaaagaa ttataaggag
    22261 tgagtacaaa tgacctataa ccatcttaca ccaactgaac tcgtaatgat agaagcttat
    22321 ttcaatcaat cacaacctgt ttcaaaagta gccaatctgt tacaacgttc tcgacaaact
    22381 atttataagg tctatcgttt tcttaaatct ggtggaagcg caatgagcta ctataaacaa
    22441 tataagaaaa acaaacgtaa ctgcggaaga catccgattg ttttacctga tgagcaacag
    22501 gaatacattc aaaaaaaggt tgttcaaggg tgggctcctg atgtccttat tggtcgagca
    22561 gagtttccta ttgcttgttc ggtacgcaca ctgtatcgaa tgtttaaaga taaaaccttt
    22621 gacactcacg atttaccaat gaagggaaaa agaaaaccaa acggtcataa agaaaaaaga
    22681 ggaaaacaag ggtttcgccg gtcaatccga gacagaaaga cagactatac ccagtttgat
    22741 catgaatttg gtcacctgga aggtgacact atcgtgggtg gaaaacataa aagcgcggtc
    22801 atcacattag ttgaacggct atctaaagtc atcattacct tgaaacccga aggcagacag
    22861 gctaaagaca ttgaaaatag gctgaatcaa tggtttcgtt gtgtcccaaa aaacttattt
    22921 aaatccatca catttgattg tggaaaagaa ttctcaaatt ggaaaaagat tagtaatacc
    22981 aacgatattt caatttattt tgcggatcca ggaacacctt ctcaacgagg attgaatgaa
    23041 cattcaaatg gcttattacg taaagatggt ttgtataaag ctatggattt taatggtgta
    23101 tctgaaacct ttatccaatc ggtggcatcg aaacgaaatc atattcctag aaaatcatta
    23161 cactatcgga caccaatgga agtatttttg agttacgtca caaaagatga tttgtctaac
    23221 ttaatttgac aaacgaaact taataaaaaa ggaagaatat tcatgtaaac gattttattg
    23281 tttttttctc ttttatacaa aaagggtcat aatttcaagt aagcggatga attcatgagg
    23341 attttttgca tactgtctct actatactta ggtcactaag aaaaaggagt gatttattat
    23401 gaaaaagaac atgattggga cattattact ttcaacactt cttctaggcg gcttggcaac
    23461 accagctttt gctgagggtc aagcgacttc aaaaggagat attaccttta ctgaaccaac
    23521 aaataccgtg gagcctctta accctactga tccttcaaaa ccagtagagc cagcagatcc
    23581 ggaaaatcca gcaactggcc agacaggtcc tttaacactg gatgtagttc ccgaattgcc
    23641 atttggtaca cacgagattg aaagcggaac aaaaacgtat caagtagacg cttcaaaaaa
    23701 cgatacacct tatctccaag tatcagatcg tcgaggtgta ggtgcagatg gccaagcaca
    23761 agggtggaac gtgacagttt cggtttcaga ttttgtaaac ggttcacaag ttttgcaagg
    23821 cgctgaatta gattttggaa catccactgt taaaagtacg tcagataacg aatccactgg
    23881 accaactagt caaactgtaa ctgggttgag taaagcaagt gcagcgacac caatatttac
    23941 agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt tatgatccag caaatatcac
    24001 actgaaagtg ccgaaagcag cagctggaac attcacggct gatttgactt ggaacctcgt
    24061 ggctggccca gtagcataga gtatgaaata aattcaacaa actgcggtga aaatactata
    24121 ggtgaaggat tttttaccgc agttgttgtt gaaaggatgt ggtggacaat gaatcaaagt
    24181 aaacgactgt ttgtttcgtt tttttctatt ttaagtctat ttttcatttt tccgtcaatc
    24241 agccaagcaa aggattcagc aggagacttt ggaatcaaac cggtatttcc agagaaccaa
    24301 atagataaag caatcggtta ttttgattta ttagtggcac cggaacaaaa tcagacacta
    24361 gaagtaatta tcagcaattc ttcagatgaa gaacgaacat ttgaggtctc tgtcaatccg
    24421 gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa aaaacccaac gttagatgag
    24481 acattgccgt ttgatgtccg tgacgttttg ctcattgcga aaaaggaaat caatgtatca
    24541 gcacatgcag aaacaactgt acctatagaa gtgaaaatcc cagcaaaaag ctttaaggga
    24601 cgtgtattag cgggtattca tgtttcacct aaagaagaag ctgaaacaga aaatgctaca
    24661 gaaggagcac aaatcaaaaa tcgtattgct tataatctcg cagtcgtttt acaagaaagt
    24721 caagagacga tagaaccgga tctgaaactt ttatcaggag atttggacga ggtcaacgca
    24781 aagccaactg ttcagcttcg tttccaaaat cctcagccta ggattatcag caatttgata
    24841 tttacgagca agatctttta tgaaaatcag ctttatattg aaaatacgtc taatgcattt
    24901 ttagttgcac caaattcgaa ttttcacttg aatcttgatt tagctggtga taaagcaaaa
    24961 gccggcgatt accgcgcaga aatcatagca aaatcaggcg acagtaatga atggcgattt
    25021 acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag taaatgaaaa ttccgttttt
    25081 gcagtacaag aacaatcttt cccatggctt tatgcaggat tggttgctgc agttctagtg
    25141 ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa agaaaaaagg agaaacaaca
    25201 tgatgaggtg atttcgttga aaacatcgat cttattggga gggattattg caagtttgct
    25261 gttcttcagc tggcaagagc cagtccaagc agataccaaa agttcaggta cgattacatt
    25321 ttatgatcta agtccaattc cgcatccaag tggttcagtc aatggcgcat caaaacagca
    25381 gacacagcta ccgatttata aagaatctaa tcaacatggc gtattcccgg agacgaatga
    25441 acaaaagatc cgttttcttt atttgatcat tggtatagaa ttatggcttc ttgcgtggtt
    25501 gttgttatgg agaagaaagg aaaaggagga tgaacatgtt gctacaaaat aaattttcat
    25561 tttttcgctc cttctttttt acttcgcttt ttgttttaag tatatttatc ttttcacctt
    25621 cggcagaagc tgttggagca acgcctcctg ccatttctta tgatccatac acgtatgaaa
    25681 atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg cgggaagctt gtccaactaa
    25741 ctggggctaa ccaattgcct aaattgactg ctacttattt gaacgatggt tcaaatgatc
    25801 cttggaaagg tggcacggtc cgttttcatt gggaaagcca tgtgaaacta acaaatgtac
    25861 ttgatttggt gaatatcact gcacaaggat ttgcatttgt ggcgatcatc aatttgccgg
    25921 aacatgtatc aagcagtgat gtattaaaag caattgactg gaacaacgcc tacttgtata
    25981 ttgccggtgc ccccatcaag gttactagcg gaagtttatt atcagcagga agccatacct
    26041 tgcgttttgg gatggggtcg tgggatacaa atactctatt gacagatatt attgctatta
    26101 ttacgcgagg cgaattcaac gtggacgata tcccgcttca atttgatgtt acagctaatg
    26161 tcgcgaagat gacagaaaac ggagataaat atgatacgag tccaaacaaa attttgacca
    26221 agggtaaatt acaaccttct atgtctaaaa aggccgtttt ttctgttgat ttttatgatt
    26281 cgaataatat tatccaagat cgtatgttca attcgatttt agctccagct gcaggtagat
    26341 tttatcctaa attaactggc ggaaacttga atccaggagc atatgcattt tctgcaagtg
    26401 cagagataaa tacgtggaat agttacatct caccaagtga ttcaacagga aaatataata
    26461 catttgctga taccacagaa tacgtggatg gaaataacca gaaccttcca ggctctacca
    26521 atagtcggac attgacgatg gggctgactg aaggacgttt tgatcagttg ccatttagtc
    26581 gctttgatcg cgtagtcaac tattttacag gaaaaaacgt tacacaagga gcaatactga
    26641 ctcatactcc agcaagcaca agtggattga atcagacagc caatatcgtg tacagtggga
    26701 aagatgcgca aggcggattg ctttctcctg ttgctttaaa ggtattggag cagtacaagc
    26761 tagacggaac ggttttacca acaaacttaa cagccgatag tcagtgggga aagctccttt
    26821 cttctgcatc gtaccaagtc acggccaatt ggtcagcaac tgaactaaaa aagggttcga
    26881 tccattacag gctttattcg aaaggaaccc aaaaattagc gacaggatgg acagatcaac
    26941 ttttccaaac gatccaatca aataacgggg gtgtaaatac agctacttct acactacctg
    27001 cattggcttc agggcaatat tattttgatt atcgcttggt ggacgatgta cttactaaca
    27061 tgtatccgtc ttttgcgtac aaatggcagt ctgaacaagc tggaatcacc actcttcctc
    27121 aaataacagt ggctaatttt ccatctgtga gtgcagatag tcagctaaaa aaccttagca
    27181 gacaacctga gacaggagat cccctgtctg ctctatcagg agatacgatt caagaaaatg
    27241 tgacattcac gttaacaaaa tctggggatg cgataaccga caaaaaaatc acgatctcac
    27301 ttcctgaaaa tactacttat atcaatggtt ccttaaaatt aaatggtaca actatcgcag
    27361 ataccggcat tcaacaagga gtttctgtcc cagcagatct attgagcaaa ataggagata
    27421 ccattcatct tacttacaac tatcaattaa atactgtaga tacttcttta caatccgtca
    27481 gcatactgac aaaggcagct gtactttcca gcaatattac attagcagat ggtgctaagc
    27541 ttcctaaccc agttgtccaa acatctgcta aaacaattct tgtccccaaa caagaattaa
    27601 cattagttaa tgtaccagat gattttacat ttggtaacga tctccccaaa ccgttgaaga
    27661 caagttatta tgaatcaaaa ggcgattttt cttttgatgt gcgggatact cggcttccaa
    27721 gcacgtctcc ttggcaactg acaggtacat tgacttcttt atttaagaat gatcaaggtc
    27781 aagaattaag cggaacaaaa ttatacttca atcattctgg atcaaagcaa ttgattcagc
    27841 aaggtcaaaa cacgttgatt tatgagagtg atggaacagc taaaggagag gttctcgttg
    27901 actttcccga taccgatggt ttactcttag aggtaaatag cagtacgaat gctcaacctg
    27961 gcgcaacgta tcaaggcatg gtcacatggg aactgacagc tgggccaacc agctaagtga
    28021 ttagaatttg tatggatctg tgataactgt cacagatcct ctttttcaag gatgagaagg
    28081 aaacttctgc tgttaaaatc agaaatgctt gaaggagtgg acttggaact tttcagtttg
    28141 cttgaaaaaa atgaacaacg gcaaatcaaa cttttccaac ttttattaga ggaaaaagga
    28201 gaggtttccg ttaataacgt acttacccgc ttatcagttg atcgggctac cttgaaagaa
    28261 gatctgtttt atttacaagc taacttggaa aatatcaaac aattggaaat tgttatccaa
    28321 gaagggaata ttttattaat cagacatgga aatatagcag cagtcgatgt ttattatcat
    28381 tgttacttgc gctattctac aaaatatcag atacttcttt atctgttgga aaatggctca
    28441 tttgatcggc agaaacttgt catggaactt aatatcagtt cggctacttt gtctcgcaga
    28501 gtgaaggaat taaatgattc tttaaatgag tttcatctga aaataaaaag cgggcggctg
    28561 accggtccag aaagtcaaat tagttatttt tatttccaat tattttggtt tggacgtccc
    28621 tatcttgaaa acaaaaaaga atacagtgat ttatcaggaa acactttttt agatctgctg
    28681 caaaaagagt ttagttttgc tattacagaa gaaggacgat tgaaatttga aatatggctt
    28741 tccattatga agaagcgaat gcgctgtacg aaaaaatcca aaccagatta tagcaatctt
    28801 cctgttggat tgtttcaaac gacacaattt ttattggacc tccatacatt tttgacccgt
    28861 tacttctttc aatatgcctt tccttggaat caaaatgaaa cgttggtttt ttatcttttc
    28921 atgatcagta actttacatt agatacacat aactcgcatg tccaggagtt tttgataagc
    28981 gaaaaataca aagacgacgt agtagctcaa ttgaatcgaa cgtttaaaca atcgatagaa
    29041 gaatatttct ctttttatgt ttttccagaa gagatttcgc aaaagattgc gttgacagtg
    29101 atgcaaaatc acgctcattt tgcctatttt agaggatgga tgaatatttt tggccaagaa
    29161 ggattaaaac agcggttaac tgaagtagaa gataaacaat tcctggtttt atgtgaaaga
    29221 ttggtcagac aaacatttgc tattgttgat tttactgaag tagacgagga aaatgcccgc
    29281 tttgaattat ttgctcgcta tgcatctatt ttacggacgc tgatcaagca gttagaatta
    29341 cgtctgcaaa ttgcttatga tcttccttat gaaaggctaa tggcggatat cgtgaaagaa
    29401 atcatccagg aacaattgct gaatacgatc aaatatgatt tagtcgaata cgagaaagaa
    29461 aagcagtatg atatcatttt gactagccaa ttgaaagagt acccgtcaca gaagtccgct
    29521 aaagtttttg tattcactag caacgaatat aattatgatt tcccctattt gaaccaattt
    29581 ttaaaagaat gttatttaga aaaattgaat ttaaggatga ataaaacatg aaaatcgtga
    29641 tcattggtgc ttcccatgaa ggcatcacag ctgccctaaa tctaagaaaa cttcaaccga
    29701 aagcagaagt tttactgatc gatgaagatc ataaagatgg actaggctat gtttcaaatg
    29761 gaatcaacct ttatttaaaa ggaaagatac gtagtttagc tgaagttgcc cataacatgc
    29821 ggactcttca agaaagtggg gcaaaactga tcaccgaatg gagagtaact gaacttgatc
    29881 cagacaaaca tcagctcatc ctcgcatcca aagaaggaaa aaacgagacc ataacctatg
    29941 ataaattaat cgtagcgaca ggttcctctc ctgtcacctt gtataaacaa attgaagctg
    30001 aaaatgtcta cacctataaa aatctagtgc aatctaagca agtacttgcc gctttaaaag
    30061 aagcaaaaga agtagtgatc ttcggtgctg gttacattgg attggaatta gcagatgctt
    30121 tgagaaataa aggatacatt attcatctag ttgattatat gccgaatgtt ttatcaagat
    30181 atttcgataa ggatatgatc aacagttttc aaaatcagct gcaaactaag caaataaatt
    30241 tttatccaaa tgaattcttg atcgattgga aaaaatcaga agagaaagta gtatccgtac
    30301 aacttctgtc tcaaacaata aaggcagata tggtcatttt ttctgcccaa actcgtccta
    30361 atacgacatt gttaaaagaa aaagtggctt tgtatgagga tgagactgtg atagtcaatg
    30421 aatatctgca gacaagtgat ccggacatct atgcgattgg agatatcgtc ccagtcagtt
    30481 ttgacaaaaa taaacggcat ctctttttac ctttagttac tcgagctgta catatggcaa
    30541 gagcagtagc gctaactttg agtggtcagc caaccgctta cgacctcaga caaaagataa
    30601 cagcagcagt cattatagac tactttttag gaactgtagg gctgacagaa gatgaagcgc
    30661 cattcttgga acaaaatacc ggttcgtgtt caggagaatt tgatctattt ccacaatacg
    30721 acgaagaaaa caaaacagta aatgcaaagc tgatctacca tccggataca ttagaaataa
    30781 ttggcggaca gctgataagc caagaattct tattatcaga tttgaacctg cttgcggata
    30841 tcgtgaaaca caaaactacg attccacagc tagcagttga ggattttgga tttctgtctg
    30901 aatacacctc tcgttttcac tacctcaatg agttagcttt taaagtacta gctaagagag
    30961 caaatagcaa tagagtagat aaaagactgt gagaaatatc acggtctttt attgtgtggg
    31021 gatataataa atattaatca atcgagaata aaaaattcat ataatgaaat agcggaagaa
    31081 ttttttgagc ataatattcg ccaaaaattt atcaagtgat aaaattgttg tataaaatga
    31141 ttatttcagg aggaggagtt aaatgttttt agcattaaat gaaattatgc attcaaagtt
    31201 acgttatgcg ttagttgcag gggtgatgtt tttgatcgcc tacctggtat tttttttgac
    31261 tgggttagct tatggcttag ctcaagataa tcgaacagca gtcgacaagt gggaggcaga
    31321 ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg atgtccatga ttactaagaa
    31381 gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt gcccaaacac ctggagtcgt
    31441 gactagtaaa gacagtacag aagaaggaaa aatcaatgtt agcttcttcg gtatcgataa
    31501 aaatcaattc atcatgccca atcttgttga aggaaaagct tttgataacg atgatgaggc
    31561 agtaggcgat attagtttga aagaagaata tggtctagcc ataggagata cagttaagtt
    31621 atctggtagt gacaagacgt ttaaactaac tggctttacg gatcatgcca aattcaatgt
    31681 ctcaccagtt ctctatacaa ccatcaatgc ttaccaagaa atccgcttcg aaaaagaaga
    31741 tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga ggtaaaatca gtaatttacc
    31801 tgaagattta gaacagatca aaatttctaa attcatcaat gaattacctg gatataatgc
    31861 gcaagtattg acttttggat ttatgatcgg atttttgatc gttattgcgg ctattgtgat
    31921 cgggattttc atttatgtac tgacgatgca aaagattaat atctttggtg taatgaaagc
    31981 acaagggatc acaggaggat ttattgcgcg ttctgtagtg gcacaaacat tcattttatc
    32041 atttgttggt attcttcttg gtctattagg aacagtggga acatcccttg ttctacctga
    32101 tgcggttcca ttccaaagca attggctgtt ttttggcgtc attagcttgt tgatgttagt
    32161 agtagctgtg ttaggcgcac tattttctgt tcggacaatc gtaaaaatcg atccgttgaa
    32221 ggcaattgga taagaaggag gaagaacatg aaagcaatag aatttaaaga agtagaaaaa
    32281 agtttccaag atggcgacca agtagttaaa gcattgaaag aaacaaccat gagtatcgat
    32341 aaaggggaat ttgttgcaat catcggtcct tctggttcag gtaaaagtac ttttttgact
    32401 ttggcaggcg gattgcagac gcctagcagt ggctctgtat ttatcaatga tcaagaattc
    32461 agtaatcaaa aagaaagcga acgcgttaaa ctacgtttta aagaaattgg ctttattttg
    32521 caagcttcca atctggttcc ttttctgaca gtaaaaaagc agttattatt agtagataag
    32581 attaaacatg aacatcgtca aaaagaagca aacgaattat ttgagcagct tggcgtggat
    32641 aagttgatca acaagtatcc agaagaacta tctggtggag aacggcaaag ggtcgcaatc
    32701 gctcgtgcat tgtacaatga tccgtccatt attctagcag atgaaccaac tgctagtttg
    32761 gattcagaaa aggcacgaga agtagtcgat atcttggcaa aagaatcaaa agacaaaaat
    32821 aaagcaacga tcatggttac tcacgataca cggctgattg acaaatgcga tagaatattt
    32881 atgatggaag atggtgtatt gaaagaaaaa gaataaccaa aaagcaggtg gaaaaatggt
    32941 ccatcgatac aacaaagata cacagcaaaa aattttgaaa gaagcgactg acttgtttat
    33001 gtccaaggga tatctcggca cttcaacaag agaaatcgca caaaaagttg ggatcaccca
    33061 gcccaatctc tatcattatt tcggtgataa agaaaaacta tatacggcag ttttagaaaa
    33121 ccacttaaaa gatgttggaa aagctttgag agaaatcgtt caaacaagcg aaacgggatt
    33181 tcaagtaacc ttgacaaaaa tggcgcagtt tttgattgag acacatctgg tcgatttgtt
    33241 tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa acaagagatc atcttttctt
    33301 tctatggaag aaaaattatc gtgaaccttt tgaagaaatc ttttcagcaa accaatctgt
    33361 gctaagaaat gggatttcaa aagaaatcgc agcaaggcat tttttcttag tgttagcacc
    33421 ttacatcact cggtcgaaaa gacctgtgga agaaccgcta aatgtggcgc aattgatcga
    33481 tttatatttg aatggtgtat taggagaagc gtaaagctgt tatataggga gaaaatagtt
    33541 aggtgaagaa gcaagatatg acgaagatcg ttatatcttg cttctttata taggtagttt
    33601 ttcataaata caaatttgtt attctctcat gattatcagg gtatattttt atttattatg
    33661 tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa
    33721 ggaaactatt tattgggtag atgaatcacc acgaatagtc aaaaggagat ttcaggatat
    33781 gccaaaaatg aaaaaaatca ttttagcagt aatgttattt tctacggtta tatcttttac
    33841 agcccctttt ttgcttactt aagctgattt taagttttgg cccttagtct ggatctacga
    33901 ggtgagagtc aagaatgaaa aaaataattg gagcttttgt atttgcaatc attatcatta
    33961 ttatctgttt tcaaatagat gtttttggtc aaaaaaagat agttggacaa acggtcagca
    34021 gtacgaacag tacggttaca aagacaaagg aatcatttga tgatacagaa caagagacat
    34081 taatcaaaat cggaaaaaat gaccgtacac tatatacaga tgactcgcta actaccccac
    34141 ttgcaactat aaacggcgga gagctggcgg agtttctttc tgaaacaaaa aatgcttatc
    34201 agatcaagac aaatgatggc tatactggat atttagccct cgaagatgga accaaagtca
    34261 caaagaatat acaaacaaaa ccggaagagc tatctgatgc agtgattgta ttagatcctg
    34321 ggcatggcgg aaatgatact ggggcattaa gcaatgatga acaaacagaa gaaaaagata
    34381 taacattaag tacagccata aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt
    34441 atttgaccca tactacagat gaattagtac agcttggaga tatatgcgac tacagtgaag
    34501 aaaaaaaggc agatgtcttt atcagtttac acgcagactc tacggaatat gcgaatgaag
    34561 caactggaat cacgacatat tattactatg gacaagagga aacattagca cagacaattg
    34621 ctgactcctt cactgatctg cctctggatt caagaggaat ctccactggg aactatcaag
    34681 ttcttcgaga aaatcttcaa ccttctattt taatcgagat gggttatatg aataatgata
    34741 gtgatttaaa ggaattagta acagacagtt atcagcagca gattgctgca agtttgacac
    34801 aagcattgac cacttatttt cagtaataaa taagcccagc caaacaaaaa tagctcctca
    34861 tatttttcgt ttggccgggc ttattatcgc agatgcaagc ttttaaacac cgtttaagga
    34921 agaggtcgtg agcctgccgt tcaactctga gtcacaagga gcaatttcaa aaaacagctt
    34981 ctcatgtttg atgactattg tgacttgtga ccgaggagtt ggcatgagaa caccgtttaa
    35041 gaaaaaggtt gtgaaatcaa cgttttgttc tgagtattag agaaacaaac ggaaaaatag
    35101 ttcctcatat tttgccgttt gttgagctaa taccgaggat cagatgattg aacaccgttt
    35161 attcggaaca agggactgtg acatgacttt tgtcacagtt ccattatttt tttagtagct
    35221 tcaaagcttt ttctgtattg gcaaaatgaa gtttggcata tttttcaagc ataggcatgc
    35281 cgcctttctc caatagtttt gccgctactt ggtcagattt cgttccagct cctgacggaa
    35341 gggtgattcc agcttcagct gatgctttgt ctaactcttc caaaaagctg gcacgactga
    35401 tgatagaagc agcagcaaca gctaaatgat attgttctcc tttagtaacg aagtacagtt
    35461 tttcttgtaa ttgatttttt tctaaacgcg catatttttt aaaatttgct tccggcgtaa
    35521 actgatcgat caaaatcgct tctggttttt ctggctcgat tttttttagc agcaaatgga
    35581 tcgcttgatt gtgcaaagcg actttcatcc gaacggcatt gtattttggt tggatctcat
    35641 tgtatttttc tggagataag acaagcagtt tataggggat ggtttttttt aagatgtcag
    35701 caagctggat gatttggcta tcttttaact cttttgaatc tctaacacca agtttacgta
    35761 attctgcaag ctgatcacgt ttcacataag cagcacatac tgttagcgga ccgaaatagc
    35821 tgccatttcc gacctcatca ctacctaaaa cggataattg actgatattt tttggcaaag
    35881 aagaggctgt ttttgctggc ttggcttgtt tatcctgtgc ctccccccat ttatcagctt
    35941 ctattgctgc attcgtacct tgaaacatca cttttccgga tgtataagcg gtaatggtcg
    36001 tcccgtcttt ttttgcagca aagctagtat aaggaatctg tttatttaac aaataagagc
    36061 gatagtatgc tttcatttga ctaagttggt cgtttgttgc tttgataatg acgttgctca
    36121 taaaattctc ctcgttttta tacataaaac tataccgcaa ctcacgactt ttcgttttct
    36181 aagagattgc gagacatagc atttttttaa agtttatcat ataatagtta agtatgtgaa
    36241 aaatatttgt ggagaacggt gaaagaacca acttcatggt acactaaata aaactttcat
    36301 caaataggag gaaatgattc gtatggcgca cgaaaaaaca cgctacaaag cggtgattgc
    36361 caaccaaaca tatactatta tcggacggga aaccaagcat catatggata tcgtaacgaa
    36421 gctgatcaat gaacagttag cagaattgaa acaattatca ccacaaatgg ataatgaaca
    36481 agcagcaatc ttgatggctg tcaatgcgct ttcggatcaa ttgaagaaac aagaaagaat
    36541 attggagctg gaagaagaaa cagcagaatt aaagaaaaaa atgatcaaat ttacagagct
    36601 tgaaaatcgt gtgaaacgca ttgaagcaat cgagaacgaa gctcgggaag tattgaaaga
    36661 aaatggtcaa gcagatcata ccattaaaaa tcatgtggaa gcacaacaga ttttaaacga
    36721 gaaacgaaaa ggacaaatta aacaaaaatc ttctaattaa gatcctttta tcaaaataag
    36781 aaaaatttaa aaaagttaga agagaaagga acgtagcatg ctgagtttgc tgattttatt
    36841 tttattgcta attgcgtttt tttctggagc acgtcgcggg tttgctttgc aaatcatatt
    36901 cgcgattgga tatgtattgt catttattgc tgcccagcat ttttacaaac cattagcaag
    36961 tcatttggaa ttatacattc catacccagc tgtcacccca acatcaaagt tagcattttt
    37021 tgatcaggta tttgcctttc acctagatga agctttttat gcaggaactg cttttctgat
    37081 tattttgcta attggctggc tgcttacccg atttgtgggg gtttttgtcc atggattgac
    37141 gtatgtgccg atcttgagac aggtagactg gattgcagga ggcatcctgt cactaatcat
    37201 ggcttacgtt actatatttt tgattttgca gttacttgcc tttgttccat tagatgtggt
    37261 ccaaaatcaa ttcggcgagc atagtttcgc tcgttttatc gttgagagaa cgccatttct
    37321 taccaacaag atgtatgagc tttgggtaac gaatattttg aattaaggaa aatagtgctg
    37381 gaaaatattt cgttttttgt acagagtagt ttatttaaga aggctgcgta gcgtatgtcc
    37441 ggcacagctc ttttttcata gtgaaagata aggtgataaa atgaataacc gaatccttga
    37501 aacattggaa tttgaaaaag tcaaacagat ggtcagacaa tttgtagtga ctgcacaagg
    37561 aaaagaagaa ctggcagaat tggtaccagt gagtgagaaa caaacgatca caaactggct
    37621 tcaagaaact gaggatggat tgaaagtgca acgtctgcga ggcgggatcc ctatacctaa
    37681 aattgaaaat atccgtcccc atatgaaacg gattgagatc ggagcagact tgaatggagt
    37741 ggagcttgca caagtttcca gagtattgtc tacgacaagc gaattgaatc gtttcattga
    37801 tgatctttct gacagtgaaa tcgaatttgc acgtttgtat atgtgggcag atcagttagt
    37861 cacgatccct gttttgagcc gccgtttaaa ggaagcaatc gatgaagacg gacgcgtgac
    37921 tgatgacgct tctcctgagt taaagagcat aagacagaac attcggcgaa gtgaacaagc
    37981 tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat gcaaagtatt taagtgatgc
    38041 gatcatcacc atgcgaaatg accgctacgt tatcccagtc aaacaagaat accgcggtgt
    38101 atttggcggt gtagtccacg atcaaagtgc ttcaggtcag actttattta ttgaaccaaa
    38161 acaagtggtg gatttgaata atcgtttgcg tcaataccaa atcgctgaac gaaatgagat
    38221 tcaacgtatt ttaagtgaac tttctgctga acttgttccc catcgtcaag aaatcatcca
    38281 caatacttat gtgattggta aaatggatct gatgaatgca aaagcacgat ttggtaaaga
    38341 agtaaaagcc atcgtgccgg gaatcagtga agacaatcat gtcgtcttga aacaagcaag
    38401 acatccactg atcgatcagg aaaaagtagt ttctaatgac atcacaatcg gaaaagacta
    38461 tcaagccatc gtcattacag gacctaacac aggtgggaaa acgatcacgt tgaagacttt
    38521 aggtctgtta caactgatgg ggcaagcagg attaccgatt cctgcaggtg aagaaagtca
    38581 aatcggtatt tttgaggaag tatttgcgga tataggagac gaacagtcga ttgagcaaag
    38641 tctatcgacc ttttcttctc atatgacgaa cactgtagat attttatcaa aagtcaatga
    38701 aaaaagtctg gtattatttg acgagctcgg tgcaggaaca gatccgcaag aaggtgctgc
    38761 tctagcaatc gcaatcttag atgatcttgg taaaaagtct gcttatgtga tggcaacaac
    38821 gcattatcct gaattgaaag tttatggata taatcgggca aatacgatta atgccagcat
    38881 ggagtttgat gtggatacat taagtccaac gtatcgtttg ctgattggcg ttcctggacg
    38941 aagcaatgcg tttgaaatct ctagccgatt aggtcttgat acagaagtta ttgatgaagc
    39001 gaagcagttg atgaatgatg agagccaaga tttaaacgaa atgatcacag atttagagaa
    39061 tcgccggaaa atggcagaga ccgaatatct ggaaatgcgt cattttgttt ctgaggcaca
    39121 agaacttcac gatgatttga aagaagctta cagctatttc tttgaagaac gtgaaaaaga
    39181 aatggaaaaa gcaaagaaaa aagcaaatga agtcgtgtca gaggcggaag aaaaagcaga
    39241 aaaaatcatt gctgatattc gtaaaatgca acagcagatt gggcaaggaa atgtgaaaga
    39301 gcatcagttg atcgatgcaa agacccaatt agcaaatcta catcaagaag aaactttgaa
    39361 gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg ttgaaaccgg gagacgaagt
    39421 gttggttacg acttacggcc aaagagggac gcttttacgt aaaaacggga atcagtggca
    39481 agtagaaatc ggtatattaa aaatgaatgt gtccgaagac gagctgacgc ctgttgctcc
    39541 tcaaaaagag ccgacacaac gagttatcca tgctgtacga tctgaatcta gtagtcacgt
    39601 gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa gcgctaagtg aggtagatca
    39661 atatttggat tctgccattc tagctggtta tcctcaagtg actattgtcc acggaaaagg
    39721 aacaggcgca cttcgcaagg gaattactga ttatcttaaa aaccatcgga gcgtcaaaag
    39781 ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg acaattgtta aatttaagta
    39841 aagcttacta aaggtaagca gatggttcgc tatccaagta tagaaatgct atactagtca
    39901 tagattttaa aaatggaggt ctttgatatg tcacaagtaa ttacagataa gacatttaat
    39961 gaagaaacag ataaaggact tgtattgatt gatttttggg caacttggtg tggtccatgt
    40021 cgtatgcaag caccgatttt ggatcagttg gaacaagaat atgatgaaga agaattcaga
    40081 attgctaaaa tggatgtgga tgaaaatcca gaaactcctc aacaattcgg tatcatgagc
    40141 attccaacat taatgttgaa aaaagatggt caagtagtag aaaaagctgt tggtgttcat
    40201 tcaaaagaac aactacgtca aatgatcgat caatatttat aaaaacttac agcaaaaata
    40261 agctgagggt gggactgaat ccagttccgt cctcggtttt tttgcggttg agttaaggtt
    40321 attcaaatta atattttttg agtataatta cgttgtcagg ctggattgtt tttttgacag
    40381 acatgctata ataactttaa ttgaatatga aggggagtac cagatgaacg aacgaatcaa
    40441 aaacaaatta gctttacttc ctgatcagcc cggctgttac ttgatgaaag ataaaaatgg
    40501 aacgatcatt tatgtcggca aagccaaaat attaaaaaat cgtgtccgct catattttac
    40561 tggtagccat aatacgaaaa cggaacggct tgttagtgaa atcgaagact ttgaatacat
    40621 cgttacagaa tcgaatatcg aagcattgct cttagagatc aatctgataa agaaaaatga
    40681 tcctaaatac aacattatgt taaaggatga taaaacctat cctttcctta agataacgaa
    40741 tgaaaaatat ccccgtttgg tgattactcg taaggtacta aaagacaaag cactgtattt
    40801 tggaccatat cccgatgtcg gtgcggcgaa tgagacaaag aagatcctcg atcgattatt
    40861 tcctctgaga aaatgcaaac ctagtcagac gaaggagcct tgtctttact atcatttggg
    40921 acaatgcctg tgtccttatt atttcgacgt agatccgaaa gtctatacga aaatcgttga
    40981 tgaagtgaaa aactttttaa acggaggaca cgaaaaaatc gagtcagaga tccatcagaa
    41041 aatggaaaaa gctgctgaaa acatggagtt tgaaaaagct gcagaatacc gagaccagat
    41101 ccgtgccatt gagacaatca tgacttgcca aaaaatgaca aatactgatt tactggatcg
    41161 agatgtattt ggttatgcga ttgataaagg atggatgtgt gtccaagtgt ttttcgtaag
    41221 acaaggaaag ctaattgaac gagatgtttc aatgtttcct ttctataacg aagcagaaga
    41281 agatttcttg acttacctcg gtcagtttta tcaggaaaat gagcatttca ttcctaaaga
    41341 agtcttgatt cctgatacga tcgacaagcc cagtgtagaa gctttgttag ctacgaaagt
    41401 gctgcaacca aaacgaggag aaaagaaaaa attagttgaa ttggcgagta agaatgcctc
    41461 agttgctttg aatgaacggt ttgatctgat tgccagaaaa caggaacgga cgatcggagc
    41521 agttgaacgt ttaggaaacg cgatgaatat cccgacccca atacgtatcg agtcttttga
    41581 taactctaat atcatgggga cgaatcctgt ggctgcgatg gtcgtattca ttgatggaaa
    41641 accggcaaga aaagaatatc ggaaatataa aataaaaaca gtcaacggac cggatgatta
    41701 tgcatcaatg agagaagtca tctatcgcag atattcgcga gtgattcgcg aagggttgcc
    41761 tttacctgat ttgatcttga tcgatggagg aaaaggacaa gtagatgtgg caaaagatgt
    41821 cttagaaaat caattaggaa tcgatattcc tattgctggc atggtaaaaa acgataaaca
    41881 taaaacaaat gagttgctat tcggtacaga tttggctgta gtaccgcttg agcgaaattc
    41941 accagagttt ttcttactcc agcgaatcca agatgaagtc catcgattcg caatcacttt
    42001 ccatcggcag ctacgcagta aaaacagttt tgcttcccga ttagatgaaa ttgaagggtt
    42061 aggaccaaaa cgaaaaaaaa tgctgcttaa agagttcaaa tcgttgaaaa atatcacagc
    42121 agccacgaca gaagaattac aagaaatcgg actgccgaaa aatgtcgcac aaaacgtatt
    42181 tgataaatta catcaggaat gaggaaaggg cactttacaa agaaagtaaa gtgtcctatc
    42241 attttatgta caacaaaaag agcgtttacg ttttgttggt aaataataaa tcgcttacgt
    42301 ctggtgtcta aggatatatg tgatttcttg aagaaggagt gaagaaatgt tagaagtcaa
    42361 ggatcttgtt aaaacttttg ggaactacac agctgtcgat catgtctctt tccaaatccc
    42421 cgatggaaaa atcatggggc taattggcca aaacggggct gggaagacaa cgacttttcg
    42481 tttgatcttg gattttttga cacaagacca aggagaagtg ttatggaatg gccatcatct
    42541 tggggagaaa gattatgatg tcatcggtta tcttccggag gaacggggac tttatccgaa
    42601 agtaacgatt caagaccaac tgctttattt tgcagaactt agagggaaat caagaaaaga
    42661 gattgaacca aaaatcgact tttggatgga aaaatttcag gtaaaaggga aaaagacgga
    42721 taaagtaaaa tctttgtcta aaggaaatca gcaaaaagtc cagctgatcg ccacgttaat
    42781 ccatgaacct aaattgatca ttttggatga gccattcagc gggttagatc cggtaaatgc
    42841 tgaattgcta aaagatggaa tattggaatt gaaaaaacat ggttcatgtg tcattttttc
    42901 tagccacaat atggataatg tagaaaaaat ctgcgatcat ttgatcatgt tgcgaaacgg
    42961 aaaaatggtt ttgaatggaa aagtacatga gattcgtgaa tcatttggcc gtacgaagtt
    43021 attcttggaa tctggtttaa gccaacagga agtagaagaa atcgaaggcg tgaaaaaagt
    43081 gatccttcat gaagacaata cgttagagat taccttagaa gatcctgaag ctgggaaaga
    43141 aatctttact cgtgcgactc agttcggcta cattcctatg tttaaccagc aaccgccaac
    43201 tttggaagag attttcaaat tgaaagcagg tgaaatcgat gcgtaagttt tggattatta
    43261 ccaaagatgt ctatttaaaa aatgttaagt cgatttcttt cttgatcatg atattagtcc
    43321 cttttatcat gctgggtatc atttatgtag caggaaactt tgctcaacaa aatagtgatg
    43381 tcaataaaat cggcgtattg gctgataatg agcaaatcag tcagcaaatg agccaagtca
    43441 aaacagatga ttttgagttc aaagcagttg attctgaaga aaaagcacaa aaagagcttt
    43501 cggaagaaaa aatcgatgcg tatttagtag tctcaactga gaatgataag ataaatggga
    43561 tgctttatag cgaaaattca ttaggacaaa gtacacaact actgatacaa caacaactga
    43621 ctggcttgca atcgatgctt cgagcgagtc aattaggcat ttcaccagaa gaagtagcta
    43681 gcttgagcca acaagcaagt ttgactcgac aaaaagtcaa ttttgattca aaaggaaaaa
    43741 tgacagtcgg agaagataac tcgacagtcc aatacgtcgt aagttatgta gcaacgattg
    43801 tcttgttcat tattattttg acgtatgcgc agatcattgc acaagagatc gcttctgaaa
    43861 aaggcacgcg gatcatggaa gtgatcctgt ccagtacgac tgcacaaaag catttttacg
    43921 gaaaattaac aggtgtttta cttgttgcat taacgcaaat ggcattatac ggtattattt
    43981 ttggtatagg atacagtcaa ttcaaagata tggaaatggt caaaaatata ttagacggta
    44041 tctcattgga taacttgatt ggaccattct tgtggtattc tcttctattc atgatctttg
    44101 gcattttgat tttttctgtt ttagcagctt tatgtggctc tttggtcaac aaagcagaag
    44161 atacagcaaa agcaattttg ccagtgactt atctatctct aggcggatac atgcttggat
    44221 tgatactagg agcttcagat ccaaacaata tcgttattcg gatcacttcg tatattccat
    44281 tcttatcttc atatattatg ccgattcgct tagcaaatga gacagtcggt atgggtggag
    44341 cattgatttc cttgttggta cttgtagccg taacattctt attgatgatc ttgtctgcaa
    44401 acatgtacaa atcgaatgta ctcgtttata gcgaaggcgg attatggagt tcattgaaac
    44461 aatcgatttc aattatgagg aatgaacgaa aaaaagcata aaacaatcct aaaatacaga
    44521 aggaagctgg acatctattg ttcccagctt ctttttgtat agacctattt tatttatttt
    44581 actatattgg atatatattc attgaaaata gtgtataaca actttataat attgagtata
    44641 ccaatttttg gaggcgtttt aatggaccgt tatttagatt tactttcaga aaaattcccc
    44701 tcaacggagg atgtcatcac ggaaatcatc aatctcgaag caattttgca gcttccaaaa
    44761 ggaacagagt tatttttgag tgatatacat ggcgaatttc ctgcttttga tcatattctg
    44821 cgtatcggtt caggaaatct taaagaaaag atcaaagacc ttttttctga acaaatgacg
    44881 gaaacggata tcagccaatt cactattttt acggcttatc cagaatatgc attaacgact
    44941 gattggtaca aagaacaaga taaatcgcag ctgatccatc atttgatcga tcttttacgg
    45001 tttacgacag tcaaatatac ccggtcaaaa gttcgaaaag gattgccgaa agaatatagc
    45061 tatatcattg aggaactctt gtatatcgat gaccgtatca atggaaaaaa agactatgta
    45121 aaaaaaatca ttgaacaact tgttttgatg aaggaagaag aaaaattttt aacgaaactt
    45181 gcacagacga tccaaaaatc tgtcatcgat catttgcata tcgttggaga tatttttgac
    45241 cgaggaactc aagcagccaa agtgatggat cgaatcatgg atttttcttc tgtagatatc
    45301 caatggggaa atcatgatat tttgtggatt ggtgcgtatt gcggctctga ggcatgttta
    45361 ctgacacttt tacggattgc agcacgctat aattatttat ttgaattaga gaaagaatat
    45421 gggttgaatt tacgtccatt gttttctttt gctcatcaaa cttatcagaa gaatccagta
    45481 tttgcgccta aaaacagtaa aaatctatcc gaaagagagc aagaagaatt agaaaaaatc
    45541 catcaggcac tttctatcct tcaattcaaa tcagaacatc agttattgga tcgtcgacct
    45601 gaatttaaaa tggacgatcg taaattgctg gaaaaaatcg actatgaagc atcaacgatt
    45661 gatttagaag gtcagctcta tgagttgaaa ggaacttgtt ttcaaacgat ccagccagat
    45721 gatccaaaaa aattcttgga agaggaacaa aaagtgattg atagtttgat gtattcattc
    45781 cagcactctt tgagaatgca aaaacatatg acgtttttga tcgaaaaagg gggaatgtat
    45841 ctcacgaaca atcagcatgt gctgtttcat ggctgtatac ctgtagacga gaagggacag
    45901 ctcttagcat ttgaattaga ccaatcttat cgcggaaaag aactgctggg cttttttgaa
    45961 aaacagataa ctgaaagcgc aaaagatctg actgcaaaag aagatttagc aaccgatttg
    46021 atttggtatg cgtggagtgg gccattttcg cctttattcg gcaagagtaa aatggcgact
    46081 tttgaacgtt attttctcac agagcctcat acgcacgtcg agaaaagcaa tccttattat
    46141 cacttgcgag atgaagagtg gttcagtgaa aaaattttag cagaattcga cacaaaagaa
    46201 gaagggtcag caatcattaa tgggcacacg ccagtaaaag taaaaaaagg ggagtcaccg
    46261 atcaaagcaa atggaaaagt atttgtcatt gacggaggtt tatccaaagc ttatcaaaaa
    46321 acgacaggaa ttgccggtta ttcactatta tgcaattctt atggtttcca aatcgtgaca
    46381 cattatccgt ttttgcctat tgaacaacaa tttgcaaaaa aatccgacca aacaaatgtg
    46441 aaaaaagtaa tcgaacagca actaccacgc aaactcaatc gtgatacaac aaaaggtatg
    46501 gaactgcagc agcagattac ccagctcaaa aggctgttgg actatcgaaa ggacgattga
    46561 gaagaattcc agtgaacatt gttccataga ctggagactg agacaaaagt cgtgtcgcag
    46621 tctctttttc cggataaacg gtgttcttct gccaactcct cggtcacaag tcacactatt
    46681 caacaaacat gaaaagctgt tttttgaaat tgctccttgt gactcagagt tgaacggcag
    46741 gctcacgacc tcttggtata aacggtgttc aaaagccagt ttctcggtat taagccaaat
    46801 ttatgcaaaa atgtgaaaag ccattttcac aaaatttttc ttaatactcg aaactaaaca
    46861 gctttttcac aaccccctca gaaagtctat taaaattcat tcacaaagaa atcgaataag
    46921 cgctgttcag atggatgtac acggtgggta agttccggat gccattgaac accgaggatt
    46981 ttatgcatat catctttgct ttgaaccgct tcaacgagtc catcgttaga aaaagcgatg
    47041 gcttttaata aaggtgaaag atctttgact gcctgatgat ggtaagaatt cacaaaatat
    47101 ggatcgggga gaaggttgct taacaaactg tctgaaacaa tcttgacttc gtgagtcgta
    47161 aattgcgggg cagtgggctg ttgttcatgt tttactgacc actttggata taatgaaaga
    47221 tcttgataaa gtgtaccttc caaaacgaca ttcaatagtt ggagtccccg gcagactgca
    47281 aaaattggtt ttctttgttt caatgcttcc aagatcagtg cctgttcaaa ttggtctcgt
    47341 tgaatatttg tttccgttag ctttggatga ggatcttcca tgtaaagttg tggactgaca
    47401 tcttgaccac ctgcaagaat aagtttgtcg atttgtccaa tcagttgagg agctgaatct
    47461 ggagacatga ctggcagaat aagaggcaat ccttgagcat ctaaaactgc atccacgaat
    47521 ccttgcggtg tatagctcac ttgatttcct tggaaagtat ctgttgctcg aatcagttga
    47581 tttccggcaa tgccgatgat tggtttcatt tgtattcccc ctaaagtaga cttattgtaa
    47641 caaggattta aaaaaaatta aactatgtaa gacttgcccc tatacttatc tgttcttgct
    47701 gtgttactat ctgtaaagag aaatttcgtg tgaaattcat gacgatagtt gaaaatattg
    47761 cttaatttgt tataataaaa agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga
    47821 tctagttctt caaggggtga gaaggacatt ttattgaagg ggcctgccta tactaagtta
    47881 tctttagagg tacatacttt caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa
    47941 aggaagtgaa gcaagtgtta gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg
    48001 aaaagcgtgc tcaaacactg cggcagcagc tgaagatcta tgcaacagaa aaagagcagg
    48061 aattggaaac gatccaaaca aactatcgac aacaactaaa acagttggtg gaagaaaaag
    48121 aacaatttga aatacaagaa ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca
    48181 aggtgcaagc aactttgaaa gatcaatatc aaaagcatca gagtcaagcg atcggtgcga
    48241 tcattgaaag ggtgaaagaa acttatggcc gtcactaaga tggaaaaagt gaccctcatt
    48301 tcagataaaa gaaatcagga gaggatttta caagcagtac aaggaatcca aaacgttgaa
    48361 atccgggatc tgtatcaaaa tagaacaaac aaccagtggg tggagaagta ttttccagat
    48421 accacgatta ttgataaaga agcaaaatta agcgctttag gtaatcggtt gaaagacatc
    48481 caagaagcta tccaattcat acaacaccac ggcgatagcc gtcaaaagaa actccatttg
    48541 aaacgtcaag aactttcctt acaagatttg gaagccaatt attccgaaga aaatttcata
    48601 aataaattga aagaagtcct tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt
    48661 gatcgtttag aagaagagga agaatggctt cttcattggc aacagctgga tattgctcca
    48721 agtatgtatg caagtaagac gaccattttt gagatgggga ctgtgagtgc agcaaatttt
    48781 gaaagtttca aggaagaact gagtaagctt gatgacgttt atatggaaga aatagattat
    48841 acgccaaaac aagtttatct ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa
    48901 gaaattgctg ggaaatacgg gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa
    48961 gaaaaactgc aagagaccaa gcagcaatta tccattgttc acgagcagca aaagaagata
    49021 acgacagcgt taggtcagtg cagtggctat atccaagaat ttgaatgggt agaagaagtc
    49081 acacttgccg ttgcagaacg agaaaaaata aaagaacggt ttatccatgc gacttatctg
    49141 atcgttttgc aaggttgggt agatgcagaa gaaaaacaga atttgctgca tgtgttaaac
    49201 gaaaccgtat cagaagaaga tatatacgtc agtttcgaaa caccaaactc agaagagatt
    49261 cagcaggaag tccctactaa attgaaaaac catccgcttg tcgaaccttt tgaattgctg
    49321 actgaaatgt acagtctgcc aaaatatgaa gaagtagatc caacaccttg gatgacacca
    49381 ttttatcttg tcttcttcgg gatgatggtc gcagatgttg gttatggttt gctgatgctc
    49441 attgggacaa tactagctca aaaactactt gttctaccac gcgggatgaa acgtttcgta
    49501 aaattctttg agattctctc gataccatcg atcatctggg gattgatcta cagttcattt
    49561 ttcggacagt cattaccaaa agaactgttt ggcatccgct tgcctttccc aatcctatct
    49621 acgacggatg acgtcaatac gattttgatt ttatccgtga ttttcggtct gatacagatt
    49681 ttggttggat tatttgtggc agcaaaggaa aatatcagga gaaaagatta cttgagtgct
    49741 gtcagtgatg gatttgcctg gcaagggatc ctaattggga tcgttattgc gttggttggt
    49801 gccatgttga taaaaaatac agcatttgtt taccttggcg ctgggctatc gatcattgct
    49861 gccttatgta tcttgatcgt tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc
    49921 aaaggtgcgt ataatcttta cggactgacc ggctatatcg gtgatttagt cagttataca
    49981 cgtttgatgg cattgggtat ttcaggcgga agtattggag cggcattcaa tatgctagtt
    50041 gctttcatgc cgccagctgc tcgattcagt gtagggatcc ttttgatcat cgctttacac
    50101 gcactgaata tgtttttgac actgctaagt gcctatgtcc atggcgcacg attgcagtat
    50161 gtcgaatttt ttgggaagtt ttatacagga ggcggacgtg cctttgatcc gctgaaaacg
    50221 gcggaaaaat acgtcaacat caatcacaag aaaaaaaaca ataaataatt ggaggatttt
    50281 tatataatga tggattactt aattagtcaa aatggtggaa tggtatttgc ggtattagcg
    50341 atggcaacag cgacgatttt ttcagggatc ggttcagcta aaggtgttgg aatgacaggt
    50401 gaggcagcag cagcattgac aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg
    50461 caattgcttc caggcacgca aggtttgtat ggattcgtta ttgccttttt gatttttatc
    50521 aacttaggaa atgacatgtc agttgtacaa ggactgaact ttctaggtgc ttctctgccg
    50581 attgctttta caggcttgtt ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt
    50641 caaattttag cgaaaaaacc tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt
    50701 gaaacatacg cgattttagg tttcgttatt tccttcctat tagtgttgaa tgcctaagca
    50761 taaaggagga aaagcagatg aacgccattg aaaaaatcat ctctcagatg aacgaagcag
    50821 cagagcagga aagagcggcg ttagaacaag aagagcgaat gaagatcgat caaaactttg
    50881 aacaaaaacg tacgcaagtt gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat
    50941 tactcgaaaa aaaataccgt cagctaagga atcgccagca agtagaagta cgacaagaaa
    51001 atttgaatgc gaagcaggaa tttttaagac gattgtttgc agatgcggta actgaaatgg
    51061 aaaattggga tgaatctgaa caaatccagt ttatcaaaaa tgcattgtat tcattacctt
    51121 tgacaggaaa agtagcattc attgctggag aaaaatcagc agcctatctt tctcagacgt
    51181 tgttggatga atggaacaat gaacttccct ttatgatggt tttaagtgat gaaactgttg
    51241 ctgatcaagc gggttttctg ataaacgatc aaggagttca gtataatttt cttttcagca
    51301 gccttgtaca agatatccaa ggaacgatga gttttgagat tgccaatcaa ttattcgaat
    51361 aaggagggat cagtgtatgg aataccacga actaaatccc ttgattcgcg gaagagagct
    51421 tgaacttttg tccaatgaga catttgaacg gatgattcaa acaaattctg tcgaagcact
    51481 tggtgaaata ttgaaatcca cgatttacag tccatatatt tatgacggat atgaatatga
    51541 cttcgaacag aatcttttaa aggagcagag ccgtctattt cgctggttaa aggaatcagc
    51601 accggagcct gaaatcgttt ggatctatac gatgcgttat actttccata atctgaaggt
    51661 tttgacaaaa gctgagatga caaatcaaaa tctggaccat ttgtatatcg acgatggatt
    51721 ttattcatta gaaaccttga aggatgccat tcatacgcaa gtttcaaatg aacttcctga
    51781 aagcattatg gacagtatac gagaagtacg tgattatttt gaagagtcta ccattcttca
    51841 gggaattgac gtgatttatg atcgacagtt tttgacagag cagcgacggc ttgccgataa
    51901 gctaggatat caagaattgc tgacagaagt cattgctttt attgacttga ccaatatcac
    51961 aacgatggct cgaggaatat tgcagaaaag aacatatggg ttcatgacga cggtactttc
    52021 cagctcggga agtatcgaaa aagaagtatt tttgaacttt gtagataaag atatggaaga
    52081 ttacacgaat tttctattag agacagatta tgctgatttt ctgcgccctg tcatccgtga
    52141 gggaaatatt gatctcgtaa agcttgaaca gttgaaagat gattatctaa gttcattgta
    52201 tcaagaagca caaacccagg ctttcggacc actgccacta ctagcctttt tgaacgcaaa
    52261 agaggtagaa agcaaaaacc ttcgtttact ggttatcggc aaaagaagcc atttcgaatc
    52321 agaaacaatc agaaagcggg tgagacaagt ctatgacgca taagatcggt gtagttggcg
    52381 acaaagattc tgttttgcca ttcaaattgt ttggctttga tgttcgttat ggtacaacca
    52441 agcaaaccgt tcgtcaggca attgaagaaa tggcaaagaa tgagtatggc gtgatttatg
    52501 tcacagagca atgtgcagca ttgagcccag aaacgatcga acgttacaag gaacaaatga
    52561 tacctgctat cgtattgatt cccaatcatc aaggatcatt agggataggg ctggaagaaa
    52621 ttcaaaaaaa cgtagaaaaa gcagtaggtc aaaatatctt atagtatgaa aaggagatag
    52681 aaattttgca aattggaaaa atcataaaag tctccggacc tcttgtaatg gctgagaaca
    52741 tgtctgatgc cagtatccaa gacatgtgtc tagtgggaga tctaggagtt atcggagaaa
    52801 ttatcgagat gcgtggcgat gtggcgtcta ttcaagtata tgaagagact tctggaattg
    52861 gtccaggcga accggttcgc tctactggtg aagctttatc tgttgaatta ggaccaggga
    52921 tcatttcaca aatgtttgac ggaatccaac ggccattaga tacctttatg gaaatcacac
    52981 aaagtaattt cttagggcgc ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt
    53041 ggttcgaacc aacagtagaa gccggagaaa cggtgagtgc tggagatgtt atcgggatcg
    53101 tagaagaaac aaaagttata aaacataaaa tcatggtgcc aaatgggata aaaggaacga
    53161 tcaaaacaat cgaaaaaggt tcattcacta ttgatgaaac agtatgtgtc attgaaaccg
    53221 aaaatggtga caaaaatttg acgatgcttc aaaaatggcc agtaagacgt agtcggccaa
    53281 tcaaagaaaa attaaacccg gatgcaccca tgatcactgg acagcgagtg attgatacat
    53341 tcttcccagt gacaaaagga ggagcagcag ctgtacctgg tccttttggt gcagggaaaa
    53401 cagtcgttca gcatcaaatt gcaaaatggg ccgatgtgga catggttgtc tatgtgggat
    53461 gtggcgagcg tggaaatgag atgacggatg ttttaaatga attccctgaa ttgattgacc
    53521 ctaatacagg agaatcgttg atggaacgta cggtattgat tgctaataca tcaaacatgc
    53581 cagtagcagc tcgagaagct tctatctata cgggaatcac aattgcggaa tatttccgtg
    53641 acatgggtta tgacgttgct atcatggcag actccacttc acgatgggca gaagcacttc
    53701 gtgagatgag cggacgtttg gaagaaatgc ctggtgatga aggttatcca gcttatcttg
    53761 gttcacggct tgcggaatac tatgaacgat ctggaagagt cattgcatta ggatcagaag
    53821 gtcgcgaagg aagtataacc gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg
    53881 aaccagtcac acaaaataca ttgcgggtcg taaaagtatt ctggggtctt gattccagtt
    53941 tagcccaaaa aagacatttt ccttcgatcg attggataca aagctattct ctttattcaa
    54001 cagaagtagg gaagtatatg gatcatatcc ttcaagctga ctgggcatcc atggtcaccg
    54061 agggcatgcg tattttgcag gaagaacagc agctgaatga aatcgtgcgt ttggtaggga
    54121 ttgattccct ttcagataat gaccgtctga cattagaagt ggcaaaatcc attcgtgaag
    54181 attatttgca gcaaaatgct tttgatgatg tcgatacatt cacttcaaga gagaaacaat
    54241 tcaaaatgtt gaagatgatc ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag
    54301 gtgcttattt aagtgagatc atggacggta ccgtatcgat tcgtgaaagg atcggacgaa
    54361 gcaaatatat cccggaaaca gaattagaaa aactagatca aattattggt gaaatcaagc
    54421 aaacgatgca aacgatcgtt tcagaaggag gcatgagcaa tgattaaaga atatcgtacg
    54481 atcggtgaag tcgtcggtcc attgatggcg gttgaaaaag ttgctggtgt caaatatgaa
    54541 gaattaatcg aagtccgtat gcaaaatggc gagattcgtc gtggacaagt attggaagtc
    54601 caagaagata aagcaatggt ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac
    54661 tcttctgtac gatttttagg ccatccttta gaactaggtg tatcggaaga catgatcggt
    54721 cgagtattcg atggtttggg acgaccgaaa gataacggac cagagatcct gcctgaaaag
    54781 catttggata ttaacggaga agtaatcaat ccagtagctc gggattatcc agatgagttt
    54841 atccaaacag gtatctctgc aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta
    54901 ccagtatttt ctggctctgg tcttcctcat aaagaattag ctgctcagat cgcgcgacaa
    54961 gcaacggtac tcgattcatc agatgatttc gccgtagtct ttgctgctat tggaatcact
    55021 tttgaagaag ctgaattttt catggaagat tttcgtcaaa cgggcgcaat cgaccgttct
    55081 gtcatgttta tgaatttagc aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg
    55141 gctttgactg ctgcagaata tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg
    55201 acagacatga ccaactatgc agaggcattg cgagagattt ctgcagcacg gcgagaagtt
    55261 ccaggaagaa gaggatatcc tgggtatctt tacacgaact tagctacttt attcgaacga
    55321 gcaggaagaa tccgcggact aaaaggatcg gttactcaaa ttccgattct aacaatgcca
    55381 gaagacgata aaactcatcc aatccctgac ttgactggtt atattacaga aggacagatc
    55441 atcttgacta gagagcttta caagagtggg attcagccac caattgatgt actcccgtct
    55501 ctttcgcggt taaaagataa gggaactgga cccggaaaga caagagaaga ccatgcagct
    55561 acgatgaatc agctttttgc agcctatgca caaggaaaac aggcaaaaga attagccgta
    55621 gttttaggtg aatctgcttt atctgatgta gacaagatct atgcgaagtt tgctgagcgg
    55681 tttgaaaaag aatatgtcaa tcaaggattc tacacgaacc gttcgattac tgaaacactt
    55741 gatctagggt gggaactgct atcgatgttg ccaagaaccg aattaaaacg aatcaaagac
    55801 gacatgctgg acaaatatct gccagaaggg aagtgagttc tgatggcacg attgaatgtg
    55861 aatccgaccc gcatggaatt gactcgattg aaaaaacagt tgacaactgc cacaagaggg
    55921 cataaattat taaaagataa gcaagatgaa ctgatgcgtc aattcatcct gttgattcga
    55981 aagaacaacc agctgcgtca agaaatggaa gagtctatga cagaagcaat gtctgatttt
    56041 gtattggcaa atgcttcgat cacggaacct ttcatcgaag aattatttgt tttgccggcg
    56101 gaaaacgttg atttgtctat cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg
    56161 aattttgaat atgatgaaca gctgacagac acacctttgg aatacgggta tttgaattcc
    56221 agtgccgaac tggatcggtc aatcgatcgg tttactaccc tgcttccaaa acttttggaa
    56281 ctgacagaaa tcgaaaaaac ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg
    56341 cgagtcaatg cattagaata tatgacgatc cctcaattag aagaaacgat ctattatatt
    56401 cgtatgaagc tggaagaaaa cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat
    56461 atgggtacca ctgactaagt ggtatctttt tttaaaagag gaaaacgatg attaaacaaa
    56521 gcaaacaaat aaagaaacgt ttgccggctg ttcagcttct cgcattagga ttttttctcc
    56581 tgattttagt tggcggcggc ctacttaccc taccattttt tagtaatagt gggcatggga
    56641 caaattttat cgatgcgctt ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa
    56701 cattgaatac agcagagcat tggaacagtg ctggtcaatt tttgatcatg cttttgattg
    56761 agatcggcgg tcttggtttt atgatggttc cgattctatg ctttgcattg ataaagaaga
    56821 aagtcagtct cagcacacgt attatattaa aagaagcgct gaatctagaa gaagtatcag
    56881 gtgtgatgaa gctcatgctt tacatcctaa aattggcctt tgtgatccaa ttgattggta
    56941 cggttgcttt atcttttgta tttgtgcctg aatttggctg gttgaaaggt atctggtaca
    57001 gtttgttcca tgcaatctct agtttttgta atgcagggtt tgatttgtta ggagacagtt
    57061 tagccaatca ccagcaaaat gtttatttga tcatggtggt atctgctttg atcatttctg
    57121 gtgggttagg cttcatcgta tggcgtgatt tactggaata tcatcagaaa aagaagatga
    57181 cgattcattc aaaaatcgca ttaaccgtta caggtaccct tttattaggc ggattcgttg
    57241 tgttcttttt cacagagcgc aatggggcaa cgctagtgga agggaatatg tttcaacgat
    57301 tggcaaacac cttttttatg agtgtgacac ctagaacggc tggatattat tcagttgatt
    57361 acctccatat gagccatgca ggtctgattt taacgatgtt tttgatgtac atcggcggaa
    57421 catctgggtc gacggccggc ggattgaaaa caactacgtt aggtgtgttg ctgatccaga
    57481 tgcgttcgat gttgaaagga cgtacgagag cggaagcatt tggccgtacc atccgacaag
    57541 gcgcagtgtt gcgggcactt actttatttt ttgttacgtt gacgttgtgt gtagtcgcca
    57601 tcatgatttt atcggtgact gaaacgatac ctaaaacttc tggcatcgaa tatattgctt
    57661 ttgaggtctt ctctgcgttt ggtacagtag gtcttacgat gggattgacg ccagacttga
    57721 ctgcaatagg gaaattgatc attatagcgc ttatgtacat tggacgagtt ggtgtattga
    57781 ctgtggtctt ctctctgatg acaaaagcac aaagaacaca atcaaaatac aaatatccag
    57841 aagaaagtgt catgattgga taatttcatc ttctgacaga aacctagtga tttagtaaaa
    57901 taagaaaata aaataatttt tcttgcttct tggatacttt aagtgcaaaa tgttcgttta
    57961 ttttttgagg cactacccgt tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa
    58021 tcaataagat ttgattcctt tatttatcaa taggttataa ttcatatttt tttatttttt
    58081 cttttacgat acttagtcca gaagctaaaa tttcaaggtt attagaggat agggaaattc
    58141 gaacgaatgc atctggttgc gcacgtgtag taaagcgtct ggaatgataa agtcggatac
    58201 cgttagcaag aaagtcattc tcaattatct tagggtcaat ctcaggatca actggaagaa
    58261 ttcgatagta agggacacga ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct
    58321 gattggctct ttttgtaaga cttaatttag tttctaagat cctatttgct tcatttgaat
    58381 ggaggatttg agtgatgact tccacatcta atccagaagt tttcacattt atattaaaaa
    58441 ttgcttgttc tatcttgctt tggacatgta ctggaactac aagataagca actcgcaatc
    58501 cagaacaaat aaattttgtc attccagcta agtagattgt ttggttaggt aataaatttt
    58561 ggaaaggagg aatgatcgta tgttgatagt aagttgttaa aaatgaatgg atatcatctt
    58621 caataaccca tagaccttct ttttcaatca cttcggctaa ttgctctcgt cttttgtgag
    58681 agatttgaaa ccctatagga ttattacaag aaggcataag aaagacacca tgaatcttct
    58741 tttttctaca ttcctgaatc aatatatcag gtcgcattcc ttgctcgtca aaatcaattg
    58801 gaacgatctc taaatgatat aactgagcca attcaatgaa attagcgtat gtatatcggt
    58861 ctgttgctat tctatcacct ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt
    58921 gaacaccgga aacaatagat agagattcta aggacggaat gactccttga gtttttaacc
    58981 aatttgctgc agtagtcaat tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac
    59041 caacaagatt tgggttatta ctagttgatt gaataaacgg gacaacgaat ttattacatt
    59101 cttcaaaaga actaaccaga ccaaaatcaa tgatatgttc ggttacttca ttggtagaga
    59161 tggtgatgga ctcaaagaca ttgggagaaa taaatgtgcc acttccaata ttcgtataca
    59221 gcaaaccttt ctcaattcca tatttataag ccttgccaac ggtagtaaaa tttaaatcta
    59281 aataatcagc tagctctctt tgggagggta actgagtgtt tttctgtaat ttattcttta
    59341 aaatatcttg ttctaattgt tgaatcaacg atcgataata aggacgcttc aatttttgtt
    59401 tgtctggtcg ccaagttaac gggtaattgt cataagaatt aataggcatt ttctccactc
    59461 cttaaaattg tataccatac aattttacca ttgattgtat tgattttaga agttaatctt
    59521 ttgtgaggag ggaattgtct atgaaaatta ttaattttga ggaagtagta gcttctttta
    59581 gttttgaaga agcgattcaa gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa
    59641 tcagccaaaa tcctcgattg gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg
    59701 cgatgatgcc tgcttatttg ggggagaaca gatattttgg cacaaaagtc attaccgctt
    59761 ttccggataa tcatcagtat catttacctt ctcatttagg agagattttc ttgtttgatg
    59821 ctcaaaacgg actacccaaa gcattgataa acgcaaatgc agtcacctgg attcgaacag
    59881 ctgctgtatc tgcgctggca acagattact tagcaaatcc gcatgcagaa acacttgctt
    59941 taattggagc tggtcaacaa gcattgtcgc atttgactgc gatttctacc attcgtccca
    60001 tcactacagt gtttgtttac gatattttag aagaaagacg aaatactttt atcgctaaag
    60061 ctaaaaaaca gtatcctaaa ataacgttta taaatgcaat atcagtagaa gatgcagtac
    60121 gtccagcgga aatcatttgt acgttgacct ctagtaaaga ggcatttctt caaaaagagt
    60181 gggtaaggga agacgcacat atcaatgctg tgggcacatt tactcctgat acaagagaga
    60241 taagtacatc gttgatggaa acttctcaaa tctatgtaga tgattactca gctgccttaa
    60301 cagaaagcgg ggacctgctt atagccatgg cagaaggaga tttagaggaa tcttgtattg
    60361 ttggtacatt aggcgaatta gtttctgaga aaaaaacatt ttataaagga gaatcgaggc
    60421 cgacgatttt cgacgcagtt ggtctagcta ttgaagatct ttgttgtgca gaatatattt
    60481 ataacaaatt agagaaaggg gctttaaaat gaacttagat tattacgtag ttgatgcatt
    60541 tgcagatgag gtgttcaaag gaaatccagc agcggtttat gtgctagaag aatggctgcc
    60601 agaaggtacg atgcaaaaaa ttgccattga aaacaattta tctgaaacag catttacagt
    60661 gaagaaaaac caagaatttg agcttagatg gtttacgcca gatcgagaaa tcgatctttg
    60721 tggtcacgcg acattagcaa cagcatttgt tttatttaac tattacaaaa tacctgatga
    60781 aacgattaaa ttttctactc aaagcggtaa tctatttgtg acaagaatta aagattatta
    60841 ttacatggat tttcctagta ttatgccgaa aaaagtgccg atattgtctg aatatgaaga
    60901 agcgattggg gctaagatta aagaagccta cttagcacgt gatttatttt tcgtattaga
    60961 agatgaaaaa acggtggcta aattgcagcc agattttaca gctatcaaaa attttgaatt
    61021 aggaatagga gtcatcgtga cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt
    61081 ctttccaaaa ttaacaatca atgaagatcc agtatgtgga tcagcacatt ctaatcttat
    61141 tccttattgg gcagaaagaa tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag
    61201 gggcggggat ctagaatgtg aattaaaaga agatcgtgtg ataataggag gaaaagcagt
    61261 tttgttttct aaaggtacct cgtttatttg atttctttca aatgaattaa aaataaattg
    61321 gtggagagtg gcaaccttat agctacaatg gttgttaaaa aaagacgcaa actttttcaa
    61381 tttaatgaaa agtttgcgtc tcattttaat tattggtgtt tgttttttat tcaatcaaga
    61441 gtaaaatgcc ccatactaac tatcctcgga tgacttcgat tttataacca tcagggtcaa
    61501 tgacaaagta gtaagaaggc gcagtgccag gtaatccttt taagtcagta atctcaagac
    61561 cagcagcttt atgttgctcg tgtaattttt ctagatcatc agctgctata gcgatatgac
    61621 catagccgtc tcctagatca tacgcaccat gatcatagtt gtaagtcaac tctaattcat
    61681 agtcatcccc aggaagtgtc aaataaacaa gtgtaaattt gttttctgga aaatcacggc
    61741 ggcggctttc ttcaaaacca aatgcctttt gataaaattc gattgatgct tcaagatctt
    61801 tcactcgaac acatgtgtga gccattttca tagattcatc tctccttttc cttttactca
    61861 atcataacaa aaagaagtaa ggaaatagtt gatttgtgct tgttcatgag aaatttctca
    61921 tgtaaaatgg atagaaaata aaactgttaa ggtggaaact attcatggat aaaccaattt
    61981 ttggtgaaaa aaagcaattt gctgattatc aaacccgtta tgcagcatat attgtcatag
    62041 aaagagatca gcagattgca gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg
    62101 gggaaattga aggagaagag acgaaagaag aagcgattgt ccgtgagctg atagaagaaa
    62161 tgggaattgt ggcagaaatt tcttcttatc tgggagaagc tgatgagtat ttttactcga
    62221 attatcgtgc aacttattat tataatccag gatatttctt tgttgctgat tcttggaagc
    62281 gtataggaga accaactgaa aagaccaata aaatttggtg ggtgagccct caagaagcgc
    62341 ttgtgaaatt gaaacgtggt agccaccgat gggcagtcga gagatggatc gaaaaaaata
    62401 aaaactgaaa gaaagcgagt agatcagctt tctctcagtt tttttattta ttacagtaca
    62461 tattttttga ttgcttcagc tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct
    62521 gcttctttga caagagaaac ggcatttccc attgctactc ctagaccagc atattcaatc
    62581 atggataagt cgttctcgtt atctccgata gccatgattt catcttgatc aataccaagg
    62641 tgctgtgcga gattcgcaac tgccgcacct ttacttgctt ctttatttag tacttcgaag
    62701 taatagggcg cacttttgac cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca
    62761 attgctgcat ctaaaatttc tggttcgtcg atcatcatca ttttgacgat gttcatgtct
    62821 ggtgtcattt cttcaggtgt tcgatatttt agcggcatct tgactaaact tgcttcgtgg
    62881 atcgtataag cgctgatatc tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg
    62941 agatgactgc cgacacggcg tgccattact tcgatctcta ggaaatcttc atgtgtcagt
    63001 ccgtgacggg cgatgatttt acctgatttc gtttgttgta cgagtgcccc gttataagta
    63061 ataacgtaat cattatcttg aaaaaggttc aactcgttta actgttcctg gacacctggt
    63121 aagggacggc cagtgcataa aacgatcgaa acaccttttg caacagcagc ggcaatcgct
    63181 tcttttactt cttttgttac ttctcttttc tcatttaata aagtaccatc gatgtcgata
    63241 gcgactagtt tgatagacat aatttcctcc aatatatttt aatttatttc aatcaatgcg
    63301 ccattacgga tatggctggc aaattcttga taagtttcgt caaataaatc ataatgatca
    63361 cgcaaggacg gatcaaccat ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt
    63421 cccgctaatg cagcaacaag ctgactaacc tgtgataatt ctatcaaagt tccgtcattt
    63481 cgcaaaatct cgatttgtgt gcgatgtctg ccttgttctg ggcgataaaa atcataaggt
    63541 agatcgtagc tagaattcac agcagtgtaa taaacaggat tatatccgac cttttctact
    63601 aacaaagtga gttcttggac taattttgaa tcccggttgc cagaaaaagt agcagatttc
    63661 agtggtttac gattcaaaaa acgtttagct aaatcattta aaataggatc agcagattca
    63721 gaccattgag taaagtaagt gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa
    63781 gtaaagcttc cgtcaagaaa tggcagaagt aattgagaat ggagttcaaa actgctggca
    63841 tcttcttgat acaactcgtg tgcgcgatga agcagatgct ccaaaattac ttccatacca
    63901 cgggaaacag gatggaagta cacttgcaca tacatttgat atcggctaac gatataatct
    63961 tccactgcat gcatgccact catggaaaaa gcgatcccgc cttcataagg acgaatcaca
    64021 cgtaatattc gggtaagatc aaatgttcca tattctgttc cagtgaaata agcatcccga
    64081 agcaaatagt ccattcgatc tgcatcgatc tgactagaaa tcatttggac aacttgtggg
    64141 ttggggtaag ttttttggat cacactagct accttttcag gaaaaccttc ttctactcgg
    64201 ttaagaatct gatacacttc tgtttctgga gaagtgatga tctctacagt gatggcttca
    64261 tgattcgtat ggaagatatg ttcaaatgta tgagagtaag gaccatgacc gacatcatgc
    64321 aacagtgccg cacataaagt caccagccgt tcctgatcat cccagcctcc gttgcctatt
    64381 ttttcttttg aaaaatttcg agaaaagata tcgcagattc gtcttgaaat ctcataaacg
    64441 cctaatgaat gagtaaaacg gctatgttct gctccgtgaa aagtgaaaga agaagtgcct
    64501 aattgtttaa tgcgtcgtag acgttggact tctttcgaat tgatcaaatc taagattact
    64561 tgatgctgga catggatata gttatggact ggatcacgaa aaactttttc cataggcagc
    64621 atttgatatt tataaggggt tgtcataatg tagctccttt atgttattac gttgttccat
    64681 tctctggatt tggttgttcc attgctccag ctggatttct ttttttagcc agtcggtaga
    64741 atcctgtaaa tcacttgcag aatacagctt ttcaaaagct tttgcaaatg cttctttgac
    64801 ttcttttaca aaaaggggtt ggtcaagcag ttcttcaagt gttgccattg tagcaggatc
    64861 cactgcagga tagccatttg taccaaagtc ttcttttaaa gcagattgat aaaatcgctt
    64921 catgacttgc ccacgttttt cctgattacc gttgacactt aagtacatca taacagaaat
    64981 cccatttttg atccgacgct gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc
    65041 ataagttcca ggacaataag aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa
    65101 agcgtattta gtcaatagga gcatttgctc gtaagcgtca tctatggtta ttttttttgt
    65161 gtcactttgg gggaaaatca aagaaatatt caaaattcct tcatctgata cgatccctaa
    65221 tccacctgaa tttctgatca ctggagcgta acctgcatct tccagtgttt ggatcccgtc
    65281 acttagaaaa ggagttcgaa catccttcat acctaagatg actgtgcagt catattgcca
    65341 gaaatgtaaa aagatttgct ggtgttcttt agaatattcc gttaatatat ctgttaatgc
    65401 aaaaggggaa aagtcgtttt tttgataaat gtgctgatct agtcgaatcg cagggttcat
    65461 tcaaccggct cctttgtata aattcatatt ttctacaata ttatactcta aaatcaaaga
    65521 gttgttttgg aaaaaaggtt ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa
    65581 actaggaaca atgtagctat gagaagtaaa ggagaatgaa taatggaatt atcacaagga
    65641 acaccgattt caatcaaatt gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt
    65701 tattttgatt tgaaagggca aatggtgaca attggcgata cgctgtacat acgttataaa
    65761 gagattcagg aggagacggg cgatgagatt cctgtaacga tcaaattagt tccagatggt
    65821 catgtacagc tgatccgtgc aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa
    65881 cggttagaaa catcctatcg aacaccatac ggtatgatcc agattgctac ttttacgaaa
    65941 gaactgcatg ttagtttgaa agaccgtccc acagcaggga aggttcgaat cgattatgat
    66001 ctgttcatgg gaccggaaaa aatcggcgaa tattatttga ccttggattt tacagcgtaa
    66061 atataaaaga aaagtttgat tttttgtgtc tgattttgta tgataaggag tagactgtga
    66121 aaggacgtgt cctgattgga aattagtgta tttgaaggtg caaacaaaag cgaattatca
    66181 atgatcgaag tagcacacgc gatcttagaa caacgtggcg atgtcatgga tttttctgat
    66241 ttagctaacc agatccaaaa ctatcttggg aaatcagaca gtgaaattcg tgattcactc
    66301 gcacaatttt atactgactt gaatattgac ggcagcttta tctcattagg ggataatcgt
    66361 tggggcttgc gttcatggta tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg
    66421 gacgaagagg atgaagatac tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc
    66481 aatgatgatg aagacgcgat cgattacaat gacgatgacc cagaagatac agatctgaca
    66541 gaagaagatg acgatgatct gtttgatgac gatgatgatg aagatgaaga aatcgctgct
    66601 tataattctg acttgcaaga aatcggagca gatgacccga cagatgatga agaagacttg
    66661 ccaggaaata tcgaagaaga tttaagtatc atagacgaag acgatgatga tgaagattat
    66721 gaagaggaag aattttctga ctctgaaaaa gaataaacat tattttctta cgagaggttg
    66781 tgacagagac gatcagccta tcagaataag gaggtgtgac acgatttttg tcacaccttc
    66841 ttttttgtat ataaaaatcc caagtaaata cataaaaaat aaaaaaattg aaacttttag
    66901 cgcaccttgt acgtcatatt aagtgaggca gaaaggagtg gagaatgatt tttactggat
    66961 ataagaaaaa acggcagatc gaaaaatacc gtcacctgct gaatggactc atcgaaactg
    67021 attatcaaaa aatgttccgt atcacgatga attacgtaca tgataaagaa gaggccttag
    67081 atgtgatgca agatagtttt cataaagccc tgaactattt tgaaaaagaa aaagagatcg
    67141 agcattttag tgcatggttt tatcggatat tgatccgcac ggcattagat agttggcgca
    67201 gacaaaaaag gacgcctgtg gacttatttg aaattcaaga tatcccccaa cagttacctt
    67261 tagaaacttc cgttgcagag ctccatatga ttttagataa aatagacagt ccggaaaaag
    67321 agattctgat cctctacttt tttgaaggat ttcgtttgaa agaaattgcg actatcttgg
    67381 atatgaatga aaacacagtt aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa
    67441 tcctagaatg aaggagagaa gagtgccatg tattcccaaa aagaaaattt aaaaaaacta
    67501 aagaaaactt ataaagaaca agcaattccc aaagggctta aagaggagat ccgccagcgt
    67561 tttattatcg aagagcaatc atttataaaa gcaaagcgga gaaagagacg tctccaaggg
    67621 ttcggagtat ctttggctat cgtaatgatt acaacaagta gccttctttt taatagtcaa
    67681 gttcgaagct ttgcagaaga tttacccatc ttaggatcag tcattgaatt gatcttaggc
    67741 gaacgcttta ctgatcgatc tgaaaaaatc gatattcagg taccgaagat caatacacaa
    67801 aatgagaaag aaaataaaac aattcatgga ttaaatcaaa aatactttcg cgaaggacaa
    67861 gcagattttg aaaaagctga aaaagaatat ggaaattttg aaacggatca ttaccaagtt
    67921 ttgggagatt atcagaaaat cgtagacgat aaccggtttc tcgtgattga aaggcagatc
    67981 acccaaacag cagcagatag ccatgttgaa aaaagatacg acacaatcga caagaaaaac
    68041 agtgtccagc tctcacttcc acttttattc aaagatgaca cctatctatc tgttttgaca
    68101 aaagaagtca aacggcagat ggccgaacaa gtcaaggaag atccatctaa gtactattgg
    68161 actgagcaag atattcaaga agggacgata gaaaaaccaa cacttgttac gcctacacga
    68221 agtttttatt tgaacaaaga gcatcaatta gttttgactt tttctcaata cgaaattgct
    68281 cctggttata tgggtacacc agaatttgtc attcctaagt ccgtgacaaa aacgattttg
    68341 gcatcggaag attatttgga tcattagacg agaagtagtt gcgactagca gtttcaattt
    68401 tcaagtaagt acaagtaaat aataagaaga gaaaaagaga aagaagagat atagtgaaaa
    68461 gaaattattt agttatcagt attattgctt tatccgtgct tttgtttttc gaaattactt
    68521 cggtggcctc cttgatcacc gaagtaaaag gacaaacaca ctcttcggag cattcgtcac
    68581 aatctttcaa acaatcagaa caatcaacca ttaaaagcca atcaactgaa acagctaacg
    68641 tcaaagaagc cattcaagaa tcagcggaaa aggtacaaag tcagtcatca caatcaacac
    68701 aagacagtac aaaaacgagc cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg
    68761 atggaccagg agcgaccaca acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc
    68821 atgccacttt tttcgtctta ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga
    68881 cagctgaagc agggcacgag attggcaatc acacatacga tcatcagaat ctggccactc
    68941 tttctgccca gtcgatgacg gaggaagtga cgaaagcaga cactgagatc aaaaaagtca
    69001 ctggcaaaac ccccactttt gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa
    69061 cgatcatcca gcgaccgatc atcgaatggt ctgttgattc agaagattgg aagacaagaa
    69121 atcctgattt gattttgcag aagatccaag caacagtata cgatggggca attattttat
    69181 tccatgatat ctatcctgaa acgattcgtg ccgttcctca agtgatcgat tatctgaaag
    69241 agcaagggta tcgcatcacg acagttgggg atttattagg gcatccaacg gctgtcgaga
    69301 actattatgg cagaaacgat catcggccag ttcagtaagt gagacaagta tctttaataa
    69361 ataacacatt ttaataagta gttgaacaca taactacctt acttgcaaag gcttaacaat
    69421 atctatacag gtacaacaaa aaactgtcca ccaattttct ttggcggaca gttttcactt
    69481 aaaaataaag aagcaactat tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag
    69541 ggaatcgaac ccccgtctca agaaccggaa tcttacgtga tatccactac actaaggacg
    69601 caagcactgg gataattgta actaaacaga taaaaaatta caaggggatt ggacaaaaaa
    69661 atgaaatttg agcaaaattt ttcacaaaaa cagcagcaga cacagaaact agccatgacg
    69721 caacagttgc aacagtccat ccaagtttta caatataaca gtgaggagct gttggcattc
    69781 gttgagaatc aagcaatgga aaatccattg gtggaagttg ttgagccgga atggcagcca
    69841 gattacataa aagcttcttc atcttcttat gaaggagaag agacaaatta cctaaatcag
    69901 attcctgata caaaagggtc tttatttgat tcattgatcg aacaagtcca tttgaattac
    69961 cgagatactt ttttgaggaa aatcgttctt tatcttgtag aatacatcga tttgaacgga
    70021 tttttgacga ttagtttaga agaagccgga aaagagattg gagctacgcc tatccaaatg
    70081 ctcgatgcgt tgactctgat tcagcaatta gaaccagctg gagtaggtgc acgtagtttg
    70141 caagaatgtc tcatgcttca aacagaacga gatgattatg cgccggaatt agcttatatc
    70201 gtgttggaag aatgttttga ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt
    70261 ttttctgttg atctacatgc tgttcagcag atattcgact atttacaaac gttgagtcca
    70321 agccctggcc ggattttcga ccgtagcagc gaattgtaca ttattcctga tgtccgggta
    70381 ttagtggacg acgataaaaa tgtacaagtt atctcaaacc gaaaaaatca gccaaatata
    70441 cgctttcaag agagttactt caaacaaatg tcacaacaag cagataaaga aactgaaaat
    70501 tatttaaaag aacggaaaca ggaattcgaa tggttgaaga aaacgatcct acaacgtggg
    70561 gatacaattt tgcgggttgc tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac
    70621 aaggaacgtc caatcaagcc tcttacgtta aaggaagttg caactgagat agatgtgcat
    70681 gaatccacgg tcagtcgtgc agtcaacggg aaatatttgg aaacagactt cggtgtattt
    70741 gaattaaaaa aatttttcac tacacgaatt cctacgaata gtacagaaca gacagaagac
    70801 ctttcagcag atacagcgaa gaaaaagctg caggaattag tcgatcaaga agacaaaaac
    70861 aagccgttgt ctgaccagaa attggttgaa ttgctgaaaa aagatgagat agcgatctca
    70921 agacgaacag tagcaaaata tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa
    70981 cgttatgata actaatcagt caaaagacta gaaaaagtat ttcacttttt ctagtctttt
    71041 tagttgaaaa acgagagcaa aactgttaga cttacagtcg tgaggttgag atttcccttt
    71101 attgatgtga aattcgaata gttaatttct ttttttatcg tatatgggtc gttttaagtc
    71161 tacattggac gtaaagtgtc caactaagga gatcgctatg ctggatgaaa taaaaatgat
    71221 tgaatctgtt gcaccagata ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa
    71281 tatctattgg atgcagccga ttggacgtcg aagtttatcg gaaagtatgg ggatcacaga
    71341 acgagtattg cgaacagaaa cagatctttt gaaaaatctg aatttgatcg acacctcaaa
    71401 aagcggtatg acgctgacaa ctaagggcga agaagtttat caaagccttg aaaacttcat
    71461 ggatcagctt ttaggtacgc atcaaaccga acaacagttg gcagaatatt ttggtaacca
    71521 acgctgtatc gttgtttcag gaaacagcga agagcagacc aaagtggctg atgcattcgg
    71581 cgaagcgctg agcgaagccc tagaccgact acttccagaa ggcgagaata tcatcgctgt
    71641 gatgggagga actactatgg ctgtggtagc cgaacaattg tctaatctgg aaaataagaa
    71701 acggcataac ctgtttgtcc ctgcaagagg cgggattggt gaagcgataa ctgtacaggc
    71761 aaattctgtc agtgcaagaa tggctgctaa agcaaacggt aaccaccgtg cactttatgt
    71821 tccggaacaa ttaagtctgg caacttacaa ttctttactc aatgagccat ctatccaaga
    71881 agtcttaaac ttgattagtg aagcgaattg tgttattcat agtattgggc gtgcattaca
    71941 catggcagca cgtcgtaaga tgactgaaaa agaattagtc atgctgaaac aagcaaatgc
    72001 tgttgcggaa tcttttggct actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc
    72061 tagaatcggc atagaattgc aagatttgga aaaagtcccg atcatcatgg caatcgcggg
    72121 cggaaaatca aaagctaaag caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg
    72181 gctcatcact gatgaagctg ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa
    72241 atattttttt gattttcata aggaggaaat cttaaatgac agttaaagta ggtattaatg
    72301 gttttggacg tattggacgt ctagcattcc gtcgtatcca agatgtagat ggaatcgaag
    72361 tagtagcaat caacgacttg acagatgcta aaatgttggc acacttgtta aaatacgaca
    72421 caactcaagg acgtttcaac ggaacagttg aagttcatga aggttcattc aacgtaaacg
    72481 gaaaagaagt taaagtatta gctaaccgca acccagaaga attaccatgg ggcgaactag
    72541 gcgtagatat cgttctagaa tgtactggtt tcttcacttc taaatcagca gctgaaaaac
    72601 atttaacagc tggtgctaaa cgcgttgtta tctctgctcc tggcggaaac gatgttccaa
    72661 caatcgttta taacacaaac cacgaaacat taactggtaa agaaacagtt atctcaggtg
    72721 cttcatgtac tactaactgt ttagctccta tggctaaaac attgaacgac aaatttggtg
    72781 ttgttgaagg attaatgaca actatccacg cttacacagg tgaccaaatg actctagacg
    72841 gacctcatcc taaaggtgac ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta
    72901 actcaacagg tgctgctaaa gctatcggtt tagtaattcc tgaattgaac ggtaaattag
    72961 acggagctgc tcaacgtgtt cctgtaccaa caggttcatt aacagaatta gtaacagttc
    73021 ttgaaaaaga agtaactgtt gacgaaatca atgcagcaat gaaagaagct tcaaacgaat
    73081 cttacggata caacacagac gaaatcgttt cttcagatat cgttggtatg acttacggtt
    73141 cattatttga tgctacacaa actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa
    73201 ctgttgcttg gtacgacaac gaaatgtcat acactgcaca attagtacgt actttagaat
    73261 acttcgctaa cttataagat agacaaacgt acaagtgtaa attatagaca tgtacattta
    73321 aaagcgggga agcaatcgcg cttcttccgc tttttttgat aataagttta ttcaggagga
    73381 aataaaatgg ctaagaaaac agtgaaagat atcgatttaa aagacaaaaa agttcttgtc
    73441 cgtgttgact tcaacgttcc tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa
    73501 gcagcattgc caacaatcaa ctacgttcta gaacaaggcg gaaaagcaat ccttttctct
    73561 caccttggac gtgtaaaaac agaagaagat aaagaaggaa aatctttagc tccagttgca
    73621 aaacgtctag gcgaattatt aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa
    73681 caattagaag aagcaatccg caacatgaat gacggcgacg ttgtcgtatt cgaaaataca
    73741 cgatttgaag atattgacgg taaaaaagaa agcaaaaatg atacagaact aggtaaatac
    73801 tgggcttcat taggcgatgt atttgttaac gatgctttcg gtacagctca ccgtgcacat
    73861 gcttctaacg taggtatcgc ttcaactggt atcccaacag ttgctggatt cttgatggaa
    73921 aaagaaatca agttcatcgg tgaagcagta gaagaaccaa aacgaccaat gattgcaatc
    73981 cttggtggcg caaaagtttc tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca
    74041 gataaaatct tgatcggtgg cggaatgact tacacattct acgaagcaaa aggaatcaag
    74101 atcggtaact ctctagtaga agctgataaa gtggaactag caaaagaatt aatcgaaaaa
    74161 gcaggcgaca aacttgtctt gccaatcgac aatgtctgtg caccagaatt ttcaaatgat
    74221 gttgaaacac aagtaatcga aggagacatt cctgatggtt tgatggcttt agacatcggc
    74281 ccagcatcag taaaattatt tgctgataca ttacaaggtg caaaaacagt tgtttggaac
    74341 ggacctatgg gtgtatttga aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa
    74401 gcaatcgcta acttagaagg cgcaacaaca atcatcggtg gcggagactc agctgcagca
    74461 gcagaacaat taggatttgc tgacaaattc acacacatct caacaggtgg tggcgcaagc
    74521 ttggaattgc ttgaaggtaa agaattacca ggactagcag caatcaacga taaataaacg
    74581 accaagtttg ctcaattctg acgagtaatc gatcaaaagg tgaagggagt tttcatgact
    74641 ccctagccat ttttcaataa attaaggagt gtgttcctca atgcgtaaac ctattatcgc
    74701 aggtaactgg aaaatgaaca aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa
    74761 aaacaaaatc ccttcaaatg acaaagttga ttcagtcatc ggatctcctg cattattctt
    74821 acaagaatta atgacatcag ctgaaggtac agaactaaaa atctcagcac aaaactgcta
    74881 ctgggaaaac tcaggtgcgt tcactggtga aacttcacca gcagctcttg ctgatctagg
    74941 tgttcattat gtgatcatcg gacattcaga acgtcgtgaa tatttccatg aaacagacga
    75001 agacatcaat aaaaaagcaa aagctatctt tgcaaatggt atgacaccaa tcctatgctg
    75061 cggcgaatct ttagaaacct atgaagcagg caaaactgct gaatggatcg aaggacaaat
    75121 tacagctggt ttagctgatc tttcagctga acaagtgtca aacatggtta ttgcttatga
    75181 accaatctgg gcaatcggta ctggtaaatc agctgacgca caaatcgctg acgaaatttg
    75241 tggagttgta cgtcaaacag ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg
    75301 tatccaatac ggcggttctg taaaaccaga aaatattgct gaatacatgg caaaagaaaa
    75361 tgttgacggt gctttagtcg gtggcgcaag cttgcaagca gattcatttt tgtcattgtt
    75421 agacgctgta aaataattgt tgaaaatttg cgaaaaatgc aagaaatgtt tgcactaatg
    75481 agcaaaatta actaaaatcg tatatgaggg aaatccctta ggaaaaaaca aactcaaagg
    75541 agagacaaaa catgtcaatt attactgatg tttacgctcg cgagatctta gactcacgtg
    75601 gtaacccaac aatcgaagta gaagtataca cagaaagcgg agcttttggc cgcggaatgg
    75661 ttccttcagg tgcttcaact ggtgaatacg aagcagttga attacgtgac ggagacaaag
    75721 ctcgttatgg cggtaaaggt gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg
    75781 aagcaatcat tggctacgat gttcgtgacc aaatggctat cgacaaagct atgatcgctt
    75841 tagacggaac tcctaacaaa ggtaaattag gtgcgaacgc tattttaggt gtttctatcg
    75901 ctgttgcacg tgcagctgct gactacctag aagtaccttt ataccactac ctaggcggat
    75961 tcaacacaaa agtattgcca acaccaatga tgaacatcat caacggcgga tcacatgctg
    76021 ataactcaat cgacttccaa gaattcatga tcatgcctgt tggcgctcca acattcaaag
    76081 aagctctacg tatgggtgct gaagtattcc acgcattagc ttcaatcttg aaagctcgcg
    76141 gattagcaac ttctgttggt gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag
    76201 aaggttttga agtaatcatc gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg
    76261 ttgttcttgc tatggatgct gcttcttcag aattctacga caaagaaaaa ggtgtttacg
    76321 tactagctga ctcaggcgaa ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag
    76381 aattagtttc taaatatcca atcatctcaa tcgaagatgg attagacgaa aacgactggg
    76441 acggattcaa gaaattaact gacgtattag gcgacaaagt tcaattagtt ggtgacgact
    76501 tgttcgtaac aaacactcaa aaattatctg aaggtatcga aaaaggaatt gctaactcaa
    76561 tccttatcaa agtgaaccaa atcggtacat taacagaaac tttcgaagct atcgaaatgg
    76621 ctaaagaagc tggctacaca gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa
    76681 caatctctga tatcgctgtt gcaacaaatg ctggccaaat caaaactggt tctctatcac
    76741 gtactgaccg tattgctaaa tacaaccaat tactacgtat cgaagaccaa cttggtgaag
    76801 ttgcagaata caaaggtttg aaatctttct acaacttaaa aaacaaataa ttcttttcta
    76861 atgaagatac atgatcgact attgaaagat agtcgagtat gatcacttag ttaagtaatc
    76921 gaactctcta cttgctgaaa tatcagcagg tagagagt
//
LOCUS       DASJUF010000004        60599 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJUF010000004 DASJUF010000000
VERSION     DASJUF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.004"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            96..824
                     /locus_tag="U4B62_000218"
     CDS             96..824
                     /locus_tag="U4B62_000218"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN2140407.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            824..1078
                     /gene="purS"
                     /locus_tag="U4B62_000219"
     CDS             824..1078
                     /gene="purS"
                     /locus_tag="U4B62_000219"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN2140408.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1080..1751
                     /gene="purQ"
                     /locus_tag="U4B62_000220"
     CDS             1080..1751
                     /gene="purQ"
                     /locus_tag="U4B62_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN2140409.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1752..3974
                     /gene="purL"
                     /locus_tag="U4B62_000221"
     CDS             1752..3974
                     /gene="purL"
                     /locus_tag="U4B62_000221"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN2140410.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3959..5398
                     /locus_tag="U4B62_000222"
     CDS             3959..5398
                     /locus_tag="U4B62_000222"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN2140411.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5421..6473
                     /locus_tag="U4B62_000223"
     CDS             5421..6473
                     /locus_tag="U4B62_000223"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN2140412.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6470..7048
                     /gene="purN"
                     /locus_tag="U4B62_000224"
     CDS             6470..7048
                     /gene="purN"
                     /locus_tag="U4B62_000224"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN2140413.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7045..8580
                     /gene="purH"
                     /locus_tag="U4B62_000225"
     CDS             7045..8580
                     /gene="purH"
                     /locus_tag="U4B62_000225"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN2140414.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8596..9870
                     /gene="purD"
                     /locus_tag="U4B62_000226"
     CDS             8596..9870
                     /gene="purD"
                     /locus_tag="U4B62_000226"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN2140415.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9992..10906
                     /locus_tag="U4B62_000227"
     CDS             9992..10906
                     /locus_tag="U4B62_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2140416.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11039..12604)
                     /locus_tag="U4B62_000228"
     CDS             complement(11039..12604)
                     /locus_tag="U4B62_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2140417.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12597..14048)
                     /locus_tag="U4B62_000229"
     CDS             complement(12597..14048)
                     /locus_tag="U4B62_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2140418.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14062..15078)
                     /locus_tag="U4B62_000230"
     CDS             complement(14062..15078)
                     /locus_tag="U4B62_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2140419.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15298..16386
                     /locus_tag="U4B62_000231"
     CDS             15298..16386
                     /locus_tag="U4B62_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN2140420.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16440..17966
                     /locus_tag="U4B62_000232"
     CDS             16440..17966
                     /locus_tag="U4B62_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN2140421.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17968..19173
                     /locus_tag="U4B62_000233"
     CDS             17968..19173
                     /locus_tag="U4B62_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2140422.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19430..20407
                     /gene="guaC"
                     /locus_tag="U4B62_000234"
     CDS             19430..20407
                     /gene="guaC"
                     /locus_tag="U4B62_000234"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN2140423.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20671..22095
                     /locus_tag="U4B62_000235"
     CDS             20671..22095
                     /locus_tag="U4B62_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2140424.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22556..23665
                     /gene="pdhA"
                     /locus_tag="U4B62_000236"
     CDS             22556..23665
                     /gene="pdhA"
                     /locus_tag="U4B62_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN2140425.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23668..24645
                     /locus_tag="U4B62_000237"
     CDS             23668..24645
                     /locus_tag="U4B62_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN2140426.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24670..26313
                     /locus_tag="U4B62_000238"
     CDS             24670..26313
                     /locus_tag="U4B62_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN2140427.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26321..27727
                     /gene="lpdA"
                     /locus_tag="U4B62_000239"
     CDS             26321..27727
                     /gene="lpdA"
                     /locus_tag="U4B62_000239"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN2140428.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27889..28164
                     /locus_tag="U4B62_000240"
     CDS             27889..28164
                     /locus_tag="U4B62_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN2140429.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28170..28937
                     /locus_tag="U4B62_000241"
     CDS             28170..28937
                     /locus_tag="U4B62_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN2140430.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29105..30934
                     /gene="typA"
                     /locus_tag="U4B62_000242"
     CDS             29105..30934
                     /gene="typA"
                     /locus_tag="U4B62_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN2140431.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31195..32967
                     /gene="recQ"
                     /locus_tag="U4B62_000243"
     CDS             31195..32967
                     /gene="recQ"
                     /locus_tag="U4B62_000243"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN2140432.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33081..33383
                     /locus_tag="U4B62_000244"
     CDS             33081..33383
                     /locus_tag="U4B62_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN2140433.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33386..34549
                     /locus_tag="U4B62_000245"
     CDS             33386..34549
                     /locus_tag="U4B62_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2140434.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34575..38003
                     /locus_tag="U4B62_000246"
     CDS             34575..38003
                     /locus_tag="U4B62_000246"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN2140435.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38024..39148
                     /locus_tag="U4B62_000247"
     CDS             38024..39148
                     /locus_tag="U4B62_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140436.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39138..39575
                     /locus_tag="U4B62_000248"
     CDS             39138..39575
                     /locus_tag="U4B62_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2140437.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39559..39897
                     /locus_tag="U4B62_000249"
     CDS             39559..39897
                     /locus_tag="U4B62_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN2140438.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40016..40576
                     /gene="rsmD"
                     /locus_tag="U4B62_000250"
     CDS             40016..40576
                     /gene="rsmD"
                     /locus_tag="U4B62_000250"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN2140439.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40573..41064
                     /gene="coaD"
                     /locus_tag="U4B62_000251"
     CDS             40573..41064
                     /gene="coaD"
                     /locus_tag="U4B62_000251"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN2140440.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41054..42097
                     /locus_tag="U4B62_000252"
     CDS             41054..42097
                     /locus_tag="U4B62_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN2140441.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42256..42984
                     /locus_tag="U4B62_000253"
     CDS             42256..42984
                     /locus_tag="U4B62_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN2140442.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43100..43345
                     /locus_tag="U4B62_000254"
     CDS             43100..43345
                     /locus_tag="U4B62_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2140443.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43342..43560
                     /locus_tag="U4B62_000255"
     CDS             43342..43560
                     /locus_tag="U4B62_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN2140444.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            43696..44235
                     /locus_tag="U4B62_000256"
     CDS             43696..44235
                     /locus_tag="U4B62_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN2140445.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44228..46501
                     /locus_tag="U4B62_000257"
     CDS             44228..46501
                     /locus_tag="U4B62_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN2140446.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            46539..47585
                     /gene="holA"
                     /locus_tag="U4B62_000258"
     CDS             46539..47585
                     /gene="holA"
                     /locus_tag="U4B62_000258"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN2140447.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(47674..47928)
                     /locus_tag="U4B62_000259"
     CDS             complement(47674..47928)
                     /locus_tag="U4B62_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN2140448.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48328..48597)
                     /locus_tag="U4B62_000260"
     CDS             complement(48328..48597)
                     /locus_tag="U4B62_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140449.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49067..49522)
                     /locus_tag="U4B62_000261"
     CDS             complement(49067..49522)
                     /locus_tag="U4B62_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN2140450.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(49660..51588)
                     /locus_tag="U4B62_000262"
     CDS             complement(49660..51588)
                     /locus_tag="U4B62_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2140451.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(51785..52618)
                     /locus_tag="U4B62_000263"
     CDS             complement(51785..52618)
                     /locus_tag="U4B62_000263"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN2140452.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(52810..53514)
                     /locus_tag="U4B62_000264"
     CDS             complement(52810..53514)
                     /locus_tag="U4B62_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2140453.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(53537..54163)
                     /locus_tag="U4B62_000265"
     CDS             complement(53537..54163)
                     /locus_tag="U4B62_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2140454.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            54489..56864
                     /locus_tag="U4B62_000266"
     CDS             54489..56864
                     /locus_tag="U4B62_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN2140455.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(56888..57379)
                     /locus_tag="U4B62_000267"
     CDS             complement(56888..57379)
                     /locus_tag="U4B62_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN2140456.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            57566..58486
                     /locus_tag="U4B62_000268"
     CDS             57566..58486
                     /locus_tag="U4B62_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2140457.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            58640..58984
                     /locus_tag="U4B62_000269"
     CDS             58640..58984
                     /locus_tag="U4B62_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN2140458.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59174..59674)
                     /locus_tag="U4B62_000270"
     CDS             complement(59174..59674)
                     /locus_tag="U4B62_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2140459.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60202..60285
                     /locus_tag="U4B62_000271"
     tRNA            60202..60285
                     /locus_tag="U4B62_000271"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60236..60238,aa:Leu,seq:cag)
BASE COUNT        19940 a        10457 c        12992 g        17210 t
ORIGIN      
        1 tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa aaacgatgta
       61 tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa cttttgtatg
      121 aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg atagaatatt
      181 tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga aaaggagaat
      241 tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga ataaaaaatc
      301 attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa attatccctc
      361 tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta ggtgttcccg
      421 aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac cagttggatg
      481 atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg gaagaaatca
      541 gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta tttgctaagg
      601 tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga caaattttgc
      661 ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg aatgaacata
      721 tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat caagaagtat
      781 ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt tcgtaaaagt
      841 atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa aaaaagcagt
      901 ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact ttgagataca
      961 acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg acaagctgct
     1021 tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg aagtataaca
     1081 tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg ttatgggcag
     1141 ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc tctttagaag
     1201 gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg agatgcggtg
     1261 cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa gaagggaaac
     1321 cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt ttgccaggtg
     1381 tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg aaagtcaacc
     1441 ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt ccagttgctc
     1501 atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa aaaaataatc
     1561 agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt gcagggatta
     1621 cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca gtagaagact
     1681 tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac ttcggaaagg
     1741 tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag aaaaaaagaa
     1801 tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa gatatcttag
     1861 gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc gaacattgtt
     1921 cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct catgtgttgc
     1981 aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca gttgtcttca
     2041 aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct gcgacaggag
     2101 ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg ttgctagatt
     2161 cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa gaagtagttg
     2221 caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc gaaattgcat
     2281 ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt ttgattgaac
     2341 acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg tatgtaggtg
     2401 caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa tttaatcaag
     2461 aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa aagctgctgc
     2521 ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt atacaggata
     2581 tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct ggctcaggac
     2641 ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct tatgaaatga
     2701 tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag gaagaaaaaa
     2761 tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa gtaacagacg
     2821 acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca gtcgatgcat
     2881 tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga atcgtagaat
     2941 ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta ttgcttgatc
     3001 tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat gattcacaag
     3061 tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg gttagaggga
     3121 cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat ttggatccaa
     3181 aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct agcggtggta
     3241 agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca gaagtttttt
     3301 gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta ggaactcctg
     3361 ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc tatccaacac
     3421 caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact caggaattca
     3481 aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt gatggcagtg
     3541 aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt gatctatcca
     3601 ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg attgaaagtg
     3661 ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct ttcaaacatc
     3721 agctagggat atctgtacag tttgaattgt cctctgctca attatttgca gaaactcaat
     3781 caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa atgatgggag
     3841 atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt tcagcaactg
     3901 acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat gcaatcgtat
     3961 gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt ggggtcatcc
     4021 agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag gacaagaagg
     4081 tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt tggggctgct
     4141 ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag ctgcaatcgg
     4201 acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc cttttctatt
     4261 caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca atgctcgttc
     4321 ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg atacggagat
     4381 attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat taaaaagtgc
     4441 tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg caatgattgc
     4501 cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa acggagcata
     4561 cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta taagagatgt
     4621 ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt gctttacgaa
     4681 ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac cagattcaaa
     4741 catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt tagcgaaaga
     4801 atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt cagccgcaag
     4861 cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa atcaatacat
     4921 tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc gaatgaaatt
     4981 atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg attcaattgt
     5041 tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg caaaagaagt
     5101 ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga ttgatattca
     5161 aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag aatgtatcgg
     5221 tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg gtctggaaaa
     5281 agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc cgacgccgtt
     5341 atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt tcttttaaaa
     5401 gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa gctggcgtag
     5461 atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa aaaacggaac
     5521 gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta tcggtctttc
     5581 aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa ttagcagtgg
     5641 caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg tgtgtcaatg
     5701 atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca acggggaaaa
     5761 atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt gttcaaggaa
     5821 atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca ggtaatgatt
     5881 atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg acaggagaaa
     5941 cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat tcaaatgggt
     6001 actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca tcaaaactgc
     6061 cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc tatgtttctt
     6121 ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc actggtggag
     6181 ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa atcaaacttg
     6241 gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa attcctgaga
     6301 tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc caagaatcga
     6361 aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat gaaatcggga
     6421 aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga tgagaatcgc
     6481 tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc tatcgaaaaa
     6541 aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat atgttttgaa
     6601 acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat ttgattcaaa
     6661 aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg atttgatcgt
     6721 attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg ataaaagaat
     6781 catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca tacgtgatgc
     6841 ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc aaggtgtaga
     6901 tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca cgttcgattc
     6961 tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga ttagtgaaat
     7021 tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc agataaaact
     7081 gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat ttcaacagga
     7141 ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga agaagtgaca
     7201 gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat ccatggtggt
     7261 cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga aaatatccaa
     7321 ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat gaaaccagat
     7381 gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat gctccgtagt
     7441 gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta tgaagaagta
     7501 ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa actagcggca
     7561 aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt aacggattgg
     7621 gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca aacgcttcgt
     7681 tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct ggaaaaatat
     7741 tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa tatccgagat
     7801 gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt cgccttgaaa
     7861 cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg ggaagcagcg
     7921 tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg agaagtagat
     7981 ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc cccttcttat
     8041 acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact gacagtagac
     8101 tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg tggacttctt
     8161 gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt gacaaaacgt
     8221 aagccgactc cagcagaagc aaaagcatta gcgtttgctt ggagagctgt caaacatgtg
     8281 aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg agccgggcag
     8341 atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa aatggaaggt
     8401 gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata tgcagccaaa
     8461 catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga ttctattgat
     8521 atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt tagacattag
     8581 agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga acatgcaata
     8641 agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc aggaaatccg
     8701 gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca ttcagcgtta
     8761 atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga agttcctttg
     8821 ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg tccgacaaaa
     8881 gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga tacttatcaa
     8941 atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc gtatatcaaa
     9001 gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa aggtgtagtc
     9061 gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa ccagcatcga
     9121 tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga agaattctcc
     9181 cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca agatcataag
     9241 cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta ttcaccagta
     9301 ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa accagcggct
     9361 ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg tttgattgct
     9421 acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc tgaaacgcaa
     9481 gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt attagagaat
     9541 cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt ggttgcagct
     9601 gccggttacc ccgaagaata tgagacagga atggttctac cagattttct ggaagaagag
     9661 ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg agcaggagga
     9721 cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa aaaaatttac
     9781 accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat tggagaaaaa
     9841 gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata aaaaatacaa
     9901 caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg tggtacactt
     9961 ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag gtacttctga
    10021 tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg aagatccagt
    10081 aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg cagatattta
    10141 tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat caatcaaacg
    10201 agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt ttgatttttc
    10261 taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa tggattatgt
    10321 ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag tagctgaagc
    10381 gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca atcaaaaacc
    10441 aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta atcagctgca
    10501 atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata tgacagatga
    10561 agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg atatgacgat
    10621 ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg ggaacgaaaa
    10681 attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa cgacaccaac
    10741 tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga tcgccggtac
    10801 gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata tgtctcggga
    10861 agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat tgtcataagc
    10921 aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt tcagcttcga
    10981 gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat ttcgtttatt
    11041 agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa atcccttgtt
    11101 ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca aaagcaatat
    11161 ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt ctttgttctg
    11221 tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg aaatgagagg
    11281 gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg gtaaaatttt
    11341 ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt gaaggctgtg
    11401 agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa agcgagtcgt
    11461 cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct tgcgggattt
    11521 tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa cgatataatc
    11581 gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta gatacttcgt
    11641 ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa tcaactggaa
    11701 gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg gaactggcgg
    11761 tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt ctttctgcga
    11821 caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca tggaccaacg
    11881 tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca actattaaaa
    11941 atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc ctaaataaaa
    12001 acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact tggtttgctg
    12061 ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca gcaagtttca
    12121 atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga ttttttttca
    12181 attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct aaagcagtta
    12241 ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc agataatcat
    12301 ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca ctaataaata
    12361 tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac ccgtccatga
    12421 aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt gaagacagtg
    12481 ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa ccaaggacag
    12541 ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata aaaactttat
    12601 acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt gacagttgtt
    12661 ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta ataaagaagg
    12721 agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc gatccctttg
    12781 gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc gttgtctata
    12841 actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa tcctttgtga
    12901 cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa gatcattttt
    12961 aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag catatggccg
    13021 tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt ttcaagagga
    13081 atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc atgggtcata
    13141 gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc caaactgtta
    13201 taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg tgctaaatgg
    13261 tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat tttattcatg
    13321 ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa gccaaggttc
    13381 cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg gacctttctc
    13441 tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac tccaagttcc
    13501 actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct tgctaagtcg
    13561 atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac agaatctacc
    13621 tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt attgatcagc
    13681 tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa cgtactaatt
    13741 tctttaagtg tatttgtatt atttttgatc tctcgaatat cattcctaag ttcttctagg
    13801 atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc ccacatactt
    13861 tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat atttgcgatc
    13921 gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag caaagggata
    13981 acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat gcttaaattt
    14041 tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta tattctgctg
    14101 ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg tttgaatatt
    14161 tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt tacactcgac
    14221 tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc catcaatagt
    14281 tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag ctaaagcacc
    14341 gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat catttgtttc
    14401 taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga caattcggtg
    14461 aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg ttggtttacg
    14521 attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt tctctgaacc
    14581 agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat tgctgacaaa
    14641 taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga cgaaaagctc
    14701 gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg ctcgctttgc
    14761 ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga tcatatccac
    14821 tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca tgtagccatt
    14881 tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt cagtatgctt
    14941 tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg ggtcaggtgc
    15001 ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa gggctaaaaa
    15061 aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact atcttatcag
    15121 ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga aacttaaaaa
    15181 aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa aaaaatcaat
    15241 gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg ataaaaaatg
    15301 aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag cattctatta
    15361 agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg aatcgctatg
    15421 ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc ggaattagaa
    15481 aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa tcaagtagca
    15541 caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc tatcgatggt
    15601 tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt gatcgcctat
    15661 gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga taattttgga
    15721 gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga aggaaaagga
    15781 ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact aatcaattac
    15841 aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt cgtgccatct
    15901 ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac ggcgcaagca
    15961 aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc tgttttgtcg
    16021 ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg gtatgggagt
    16081 gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg cgttaaatcg
    16141 atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt agcaaaaaat
    16201 acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa tgatactgaa
    16261 acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt atctgtcgat
    16321 aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc cgatctgaaa
    16381 aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg ataagaatta
    16441 tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga atccgagcgt
    16501 tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc ggagaaaacg
    16561 gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat ggctcctatg
    16621 acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa gatagtgaag
    16681 aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt tcaataaaag
    16741 agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg gaactgacag
    16801 aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg aatcttctag
    16861 tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca ttatcaaaat
    16921 ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa gaaagcgcga
    16981 atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc attatttcac
    17041 ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg gatggacaga
    17101 cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc ggaatggtgg
    17161 gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa gtttatttta
    17221 aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata aacgaccaca
    17281 tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg ggagcgggca
    17341 gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata tccggacgta
    17401 tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt gcaaacggac
    17461 tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg gatattcggg
    17521 aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta gataaagaaa
    17581 aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca aactccatcc
    17641 atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta tccaagtggc
    17701 tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata gatgttgggg
    17761 caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa gctgtctgtc
    17821 ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat acgatgaacg
    17881 aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc ttgatgaaat
    17941 taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac ggataaaaaa
    18001 gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta cagcatggtg
    18061 atcattttag cgggactact tattgcgttt caattgatga cggatggtat tttctggcgt
    18121 ccattgaata taactaacct tgttctgcaa aatagccata tattagtact agctgcaggg
    18181 atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt gatggcgttt
    18241 gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg gatgacagtt
    18301 atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg ggtagcttat
    18361 gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg tggactgaca
    18421 caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca aaaaatgtct
    18481 acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac attgatttta
    18541 ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa tcgtcggaaa
    18601 aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt atttacagca
    18661 gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc gattattttg
    18721 atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac agtagcaggt
    18781 cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg gatcaaaaca
    18841 aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt agctggatta
    18901 gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat ggaactggat
    18961 gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg aacaatcatg
    19021 ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc gattttaggc
    19081 gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc cgttgtgcta
    19141 gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc agaaaaaaga
    19201 ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc attgtttcgt
    19261 ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct ctttttgctc
    19321 ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc cttgattaaa
    19381 ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa tgaaagtatt
    19441 tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc gttctgaatg
    19501 tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc ctgccaacat
    19561 gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt atttctatat
    19621 catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga aaaagcgtgg
    19681 attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta tagagaagtt
    19741 ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg gacatgctaa
    19801 ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt ttgtgattgc
    19861 cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg cagatgcaac
    19921 aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag gctttgggac
    19981 tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca agccgatcat
    20041 cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt ttggtgcgac
    20101 aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg aaacaaaagt
    20161 cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc aaaaaggcga
    20221 aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt tgaaagatac
    20281 actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag gaagagatct
    20341 ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta atggagacac
    20401 aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc attaagtaat
    20461 aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat tattatcgca
    20521 gatacaagct tttgaacatc attattcaga ataagggact gtgataagat ttttgtcaca
    20581 gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt aaaatcaaaa
    20641 gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa aaaacaagga
    20701 ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat
    20761 cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc
    20821 accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag caaacgtggc
    20881 agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag aatcgattgg
    20941 cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa aataagagaa
    21001 gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat gccagatgag
    21061 cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc ggaagaaaaa
    21121 gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta tttggaaagt
    21181 aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg tcagcaagca
    21241 atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt agagtcacct
    21301 tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg
    21361 atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa aaataaattg
    21421 aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa
    21481 cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat tgaagacgat
    21541 gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg aacgattgat
    21601 ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac aacgaaaatc
    21661 ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca agaaatgatg
    21721 gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa tgaagcaatt
    21781 gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact gataagcgct
    21841 tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta ctatgcctgg
    21901 attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt agatatgggt
    21961 cttggaattg caccaagtct actttttggg caagatataa atggaatcag aatcaattac
    22021 agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat tctagaaaag
    22081 tggttaacag aataatttca caaagtaaat agattaaaga atataacagc ttggatttaa
    22141 atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac taagaaaaaa
    22201 ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca acaaaactgt
    22261 ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga aaatagaaaa
    22321 aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa tgctgttaat
    22381 atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct tgtatttctc
    22441 ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt ttattcactg
    22501 tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt gacaaatggc
    22561 aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg cagatttccc
    22621 agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag tacctgattt
    22681 atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg tattagacca
    22741 acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa ctgctggaca
    22801 agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc ttcttccagg
    22861 ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag cttttttatg
    22921 gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc taccaccaca
    22981 aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt tgaagaaacg
    23041 aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac aaggtgattt
    23101 ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt atatccaaaa
    23161 caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa cacttgcaca
    23221 aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc cgcttgcagt
    23281 atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac cagtcctaat
    23341 cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc caacccgcta
    23401 ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc gcttccgtaa
    23461 atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca tcgaagcaac
    23521 taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac aaaaggtttc
    23581 agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac aaattgcaat
    23641 ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca agcaatcaca
    23701 gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg tgaagatgtt
    23761 gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt cggagaagac
    23821 cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt cggtcttgcg
    23881 ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt cgaagcaatg
    23941 gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac tagaaatcta
    24001 cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt gcattcggat
    24061 aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc ttccaaccct
    24121 tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt tgttttccta
    24181 gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc ttacgaagtt
    24241 cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat cacatacgga
    24301 gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa tatatctgtt
    24361 gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat tcaatccgtg
    24421 gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg cgttgccgcg
    24481 caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc aatcggacga
    24541 gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg gttgccaaat
    24601 gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa gaagggacga
    24661 aacaaagtca tggcttatca atttaaattg ccggatatcg gtgaaggaat cgcagaaggt
    24721 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    24781 gaagttcaaa atgacaaatc agtagaagaa atcccgtcac ctgttacagg aacggttaaa
    24841 aatgtgattg ttccagaagg taccgtagca aatgttgggg atgtattagt agaaatcgat
    24901 gcaccaggac acgaagacaa tgaaggggac agtggtgtag ccgcagaatc ccaaactcca
    24961 gctaaaccgg ctgctgaacc cactgttgat acagaatcag ctggatcgtc aagcgaagga
    25021 gtcttccaat ttaagttacc ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa
    25081 tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga agttcaaaat
    25141 gacaaatcag tagaagaaat tccgtcacct gtcacaggga cagttaaaaa cgtgattgtt
    25201 ccagaaggca ctgtagcaaa tgttggggat gtattagtag aaatcgatgc tcctggacac
    25261 aatagtgcac cgtcaactag tgcgccatca gctgaagcac caaaagaaaa agtggaaaca
    25321 tcaggttcag caagtgtcgt tgaagctgcc gatccaaata aacgtgtatt ggctatgcca
    25381 tctgttcgtc aatttgcccg tgaaaaagat gtagatatct cacaagtaac tgcaactgga
    25441 aaaggtggca gagtaacaaa agaggacatc gaaaacttct tggcaggtgg tccaagcagt
    25501 gcaccagcga aatcagaagc gccagaagcg gcagcaccga aagaagcagc tcctgcagca
    25561 gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca acttaggaga cttggaagaa
    25621 cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag caatggtcaa cagtaagcat
    25681 acagctccac acgtgacatt gcatgatgaa gtagaagtgt ctaaactatg ggataatcgg
    25741 aaacgattta aagaagttgc tgctgcaaat ggtacgaaac ttactttctt gccatatgtc
    25801 gtgaaagctt taacagcgac tgttaagaaa tacccagtat tgaatgcatc tatcgacgat
    25861 gcaaaccaag aaatcgttta taaacattac tacaatatcg gtattgcaac tgatacggat
    25921 cacggactat atgtaccaaa tgtgaaagat gcagaccgca aaggaatgtt tgctattgca
    25981 gacgaaatca atgagaaagc aaaattagct catgacggca aactatctgc agaagatatg
    26041 cgcaatggaa caattacgat cagcaatatc ggatctgtag gtggcggttg gtttacacca
    26101 gtcatcaact atccagaagt agctatttta ggtgtaggaa cgattgcaca acaaccaatc
    26161 gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga aactgtcatt aagtttcgat
    26221 caccgtatcg tagatggagc aactgcgcaa caagcaatga ataatatcaa acgtctttta
    26281 gctgatccag agctattaat gatggaagga tgataaacaa atggtagttg gagattttgc
    26341 tgtagaatta gatacagttg taattggtgc gggacctggt ggctatgtag ctgccatccg
    26401 cgctgctgaa atgggacaaa aagtagcaat cattgaaaga gagtatattg gcggtgtttg
    26461 tttgaatgtc ggctgtattc cttcaaaagc actgatagct gcaggacacc attatcaaga
    26521 gtcactagat tctgaaatgt ttggagtaac tagtgaaaac gtcaaactag attttgcaaa
    26581 aacacaagaa tggaaagaaa acaaagttgt ccatacgttg acttctggtg ttggtttcct
    26641 attaaaaaaa cataaggttg aaacaatcga aggagaagcg ttcttcgtag atgatcacac
    26701 actacgcgtc attcatccag attcagctca aacttattcg ttcaataacg caatcatcgc
    26761 aacaggttcc cgtccaatcg agattccagg atttaaattc ggaggacgtg tgttagattc
    26821 tactggcgga ttagcattga aagaagtacc taagaaattt gtgatcatcg gtggcggtgt
    26881 gattggtgcc gaattgggcg gagcgtacgc aaacttagga gcagaagtaa cgattcttga
    26941 aggctcacca caaattttac caacttacga aaaagatcta gtaaaattag tagaagatga
    27001 tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg gcgaaagaag cagtcgataa
    27061 cggtgacagc gtgacagtga aatatgcagt ggacgggaaa gaagaatccg ttactgctga
    27121 ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat gatatgggct tagaacaagc
    27181 gggtgtcgaa gtaggagaac gaggattgat cacggtggat aaacaaggac gtacgaacgt
    27241 acctaacatc tatgccatcg gtgatatcgt tccaggggcg gcattagcac acaaagccag
    27301 ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa aaagtagctg tagattataa
    27361 agcaatgccg gcagtagcct tcactgatcc tgaattggct tctgtaggta tgacgatcaa
    27421 agaagcaaaa gatgctggta tcgaagcaaa agcatataaa ttcccatttt cagggaacgg
    27481 tcgggcatta tcgcttggta aaacagaagg attcatccgt ctagttacga cgatcgaaga
    27541 taacgtgctt attggcgcgc aaatcggtgg agtcggcgca agtgatatgg tctctgaatt
    27601 agctttagca atcgaatcag gaatgaatgc tgaagatatc gccttgacga ttcatcctca
    27661 tccatctctt ggcgaaatcg tcatggatgc ttcggaatta gcattaggtt taccaatcca
    27721 tatttgattt gaataataca ggcgaaacgc aactgaaaaa tcagttgcgt tttgtttttt
    27781 agaattatca ataatattat tatgtaaact taaaaatatg acaacttctt ggtttattac
    27841 cacttcctat gctacactga agaggaatat gaaataggag agttgaagat gcaagaatat
    27901 cagtatccac ttgatttaga ttggacaaca gacgaaatgg tcattgtaat gaagatgtgg
    27961 gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg aagctttttt gaagacttat
    28021 cagcagttta aaactgttgt caaatccatt ggtgaggaaa aacgattggg acgggaattt
    28081 gaaaaagctt ccggttattc tttatatcgt acagtgcagc aagcaaaaaa aaatccaaac
    28141 aaaactttga gaatgaacgg gtgagataaa tggaacagat gattgatcat atcaagtcct
    28201 ggcttgaaga agctggtgag aaaatcaaat cttctcaaca aacattaaca atctcgcaaa
    28261 aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa catccagaat tttctaatcg
    28321 aaaaaatcca ttcctttgat ccagaggcga aaatattggc agaagaagaa gggtgtaatc
    28381 aattatcgga tctcgatgga agagtcttta tcatcgatcc tattgatggc accttgaatt
    28441 ttgtgattga aggggaaaat ttttgtatta tgctcgctgt ttatgaatcc ggcgttggaa
    28501 agcttggttt catttatgac gtgatgagaa atgaactttt ttggggtgga aaaggaatag
    28561 gcgtgtataa aaatgatgag ccattgccta gaccagcaaa taaatcctta tcggaaggct
    28621 tacttggtgt gaatagttat cttttcggtc ataataaata tggaatccga gaaattgcgg
    28681 aaagcagcat ggggacacgt atgtacggat gcgctggtct tgaactagtg gcaatcttga
    28741 aaggaaacca cattgcttat atttcaaacc ttagcccttg ggattatgca gcgggaaatg
    28801 tcttattgga agagtttggt atggtaacaa gcggtctttt tggtactcct ttgacatttg
    28861 aaaaaaggga atactattta gcagggactt cttgtgcata tgcagaaatc gcagaccttt
    28921 tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca aaaatgataa aaacgagcaa
    28981 aagattaatt ttctcgaaaa aatgaaaaca ttttactata tttcatgaaa aaagtctgct
    29041 ataattagca gtcgagtaaa atttgccctg tcataaagaa ccaataaaaa aggagttagt
    29101 tacattgaat tatagaaacg atatccgtaa cgtcgcaatt attgcccacg ttgaccacgg
    29161 aaaaacaaca ttagttgatg aactattaaa acaatcagat acattagacg cacatacgca
    29221 attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa gaacgtggta tcacgatttt
    29281 agccaaaaac acagctgtag actataaagg tattcgcgta aacatcatgg atactccagg
    29341 acacgcggac ttcggtggcg aagtagaacg gatcatgaaa atggttgacg gcgttgtgtt
    29401 ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt tttgtattga aaaaagcact
    29461 ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt gacaaacctt ctgctcgtcc
    29521 tgagcatgtc gtagatgaag ttcttgaact attcatcgaa ttaggagcag atgatgatca
    29581 gttagacttt cctgtgattt atgcatctgc actaaatggt acgtcaagct tgtcagatga
    29641 tccagcagat caagaaccaa ccatggcacc tatttttgat acaattattg aaaaaatccc
    29701 tgctcctgtc gataacagcg acgaaccatt acaattccaa gtttcattac ttgactacaa
    29761 cgattatgta ggtcgtatcg gaatcggacg tgtatttaga ggaacaatca aagtaggaga
    29821 ccaagtagca ttaattaaat tagacggaac agttaagaaa ttccgtgtaa cgaaattatt
    29881 tggtttcttt ggactaaaac gtctagagat tcaagaagca aaagctggag acttgattgc
    29941 tgtctcaggt atggaagata tctttgtcgg tgaaacagtt actcctgtgg atcatcaaga
    30001 tgcgttacct atccttcaca ttgacgaacc aacattgcaa atgactttct tagttaataa
    30061 ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct cgtaagattg aagaacgttt
    30121 gatggcagaa cttcaaacag atgtatcctt acgagttgaa ccaacaaact ctccagatgc
    30181 atggacagtt tctggtcgtg gagaattgca tttgtctatc ttgattgaaa acatgcgccg
    30241 tgagggatac gaattgcaag tatctcgtcc agaagttatt gaaaaagaaa tcgacggtgt
    30301 aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca gaagaatata tgggtagtgt
    30361 tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat atggtccata caggaaacgg
    30421 acaaatccgt ttgacattct taacacctgc ccgcggtttg attggttatt caactgaatt
    30481 cctttcaatg acacgtggtt acggaatcat gaaccatact tttgaccaat atctgccaat
    30541 gcttcctggt caaattggcg gacgtcacca aggggcgctt gtttcgatcg atacaggaaa
    30601 agcaacgact tattctatca tgagtatcga agaacgcggt acagtcttcg tagaaccagg
    30661 tacagaagta tatgaaggaa tgattatcgg ggaaaacagc cgtgataatg atttgactgt
    30721 caacatcacg aaagcaaaac aaatgacaaa cgtacggtct gctactaaag accaaacatc
    30781 tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct ttggaattct tgaatgatga
    30841 tgaatattgc gaagtaacac cagaaagcat tcgcttgaga aaacaaattt tagagaaaaa
    30901 tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta aaaggatgaa gagagaaacc
    30961 agcttgttat aaaaaatagc tggtttcttt ttttgtccaa ctcttttctg tggagaagtc
    31021 atctttgtca atggacataa attcagaaaa tacgccgaaa gacgtttaaa agacttttaa
    31081 aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg aaaggagatc
    31141 aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg gaagatgaac
    31201 gacttattaa aacaatactt tggctatgat gagtttagac ctggtcaaaa agaaattatc
    31261 cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag tgggaaatca
    31321 atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt gtccccttta
    31381 atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc cgccactttt
    31441 atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat cgatagacag
    31501 cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt tatccaagca
    31561 atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat ctctcagtgg
    31621 gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca actgccaaat
    31681 cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc tgacataaaa
    31741 agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg agaaaatcta
    31801 cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata tttaaagatc
    31861 aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga ccgtctctat
    31921 catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct aaatgagaat
    31981 gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat tgtagcgaca
    32041 aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca ttatcaaatt
    32101 cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga tggtctttcc
    32161 agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt ttttatccag
    32221 cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa agcgatgaca
    32281 gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt tggcgagaca
    32341 agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt agaagtaacg
    32401 acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta tggtaaacaa
    32461 atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt aggttttgga
    32521 catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat gcagttaatt
    32581 gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc tgttttgaaa
    32641 gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg aaaggaaccc
    32701 aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt gctgcgggaa
    32761 ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt ttcagattcc
    32821 acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct tcagataaaa
    32881 ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg gataaaaaat
    32941 gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga ataaaaacaa
    33001 ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa ttaaggaatt
    33061 agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc caagtattaa
    33121 atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt ctctgtatct
    33181 cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt tcccggcaag
    33241 tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg atgttaagtg
    33301 agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt tatgataaaa
    33361 aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc atctattaga
    33421 ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca tggtatatag
    33481 ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag tcattaacca
    33541 gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa tgaaaacaga
    33601 tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga ttttgctgct
    33661 gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc aaattgcagg
    33721 attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc tgtctctgac
    33781 tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga aacgtccact
    33841 tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg gtaatgcggc
    33901 agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt atgtgtatac
    33961 tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc tgatcaatat
    34021 cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat ttgctattta
    34081 ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg gctactatgc
    34141 catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga aaaaaggatt
    34201 tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac tgggagtgat
    34261 tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg tccttgtagg
    34321 tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg caatgttttt
    34381 gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga cagggatcac
    34441 gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt gtgtcggctt
    34501 tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat aaaaaaagat
    34561 gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc gctgtacgtg
    34621 tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca gaagaagatg
    34681 aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa ggaaaaaaac
    34741 cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa tgcggagctg
    34801 acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg caacggtgcg
    34861 aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt tttggagata
    34921 aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca ggcacagatg
    34981 gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt tatccgatta
    35041 tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac gatgaaaaga
    35101 gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt ggtagtgatg
    35161 aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag attctcgggg
    35221 atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag cgccgtcacc
    35281 aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt caaaaaatct
    35341 gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt acagtagaat
    35401 ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc caagtggaac
    35461 atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg ttgattgctc
    35521 aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt aaattaaatg
    35581 gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt ttaccagaca
    35641 cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg gatgtcggaa
    35701 atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta aaagtatgta
    35761 cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg aaagaattta
    35821 gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca catcctgaat
    35881 tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta tttgaatttc
    35941 ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt actgtgaatg
    36001 gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt atgcctaaag
    36061 atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg aagggtgccg
    36121 atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat acgacattta
    36181 gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc aagcagattg
    36241 cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg gggggagcaa
    36301 catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg agaaaaattc
    36361 gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat gccgttgggt
    36421 attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca gcgcaaggaa
    36481 tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa aaaagtatcc
    36541 aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact ggcgatatca
    36601 acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca aaggaactag
    36661 aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt aaaccgcagg
    36721 cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc catctgcata
    36781 ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa gcaggagtag
    36841 acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct agcatgagca
    36901 gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt gaaaatgcac
    36961 agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt gaaaacggct
    37021 tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag tattcgaatc
    37081 tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc aaacaaatgt
    37141 atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca tctaaagtag
    37201 ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat atttatgaaa
    37261 aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga gaactagggc
    37321 aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc agacctgcat
    37381 tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta aaaaaagaat
    37441 tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat ttgatgtatc
    37501 cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt acattactgg
    37561 acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta caaatcgaac
    37621 gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc gaagggaatc
    37681 gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa gataattcga
    37741 tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa caaattggag
    37801 caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac gttaaaaaag
    37861 gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa gcacgttttg
    37921 acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct ggtgaccttt
    37981 tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa gactgggttt
    38041 gttttggctg attctgctat tagttattgt ggcattctac atggaaccag tcttgtttcc
    38101 gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa tgacaacgtg
    38161 gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac cgatcgatca
    38221 gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg gttttgaaac
    38281 aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc aagacagcag
    38341 agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt tttatttagg
    38401 gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca cttttgagta
    38461 ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc gtccgctaat
    38521 tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa acggcctatt
    38581 ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc aagtgtttgg
    38641 tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa tcaatcgcgt
    38701 taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg aacttcccat
    38761 atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg attttttgac
    38821 aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat cgctaggcag
    38881 tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt taatcagtaa
    38941 acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg ctactttttc
    39001 gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc cgggaacgga
    39061 ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag aagaaaacgg
    39121 agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag gatcaatgcc
    39181 tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt gaacataaac
    39241 agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg tctaagaaag
    39301 aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt gaaaaaaaac
    39361 gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca gctttccgat
    39421 atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt gccgaagaag
    39481 tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa aaacactttg
    39541 gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac aaaacgacgc
    39601 gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa gttcggcttg
    39661 ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga agagacattg
    39721 gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga acgttcttac
    39781 cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt attaaaagac
    39841 gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca tctttaaaaa
    39901 tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt tttgatcatt
    39961 gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg agtagatgcg
    40021 gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg cgaacaccag
    40081 accaactaca gataaagtaa aagaatccat attcaatatg attggaccat attttgatgg
    40141 agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag cagtatctag
    40201 aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa tcatcaaaga
    40261 aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg atgcggacaa
    40321 agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc ttgatccgcc
    40381 atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg gacttttttc
    40441 taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag aaactgtggc
    40501 tggattccat caaataagaa aacagattta tggcataacg gccgtaacta tctatagaaa
    40561 agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt tacaaaaggt
    40621 cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat tggtgttttt
    40681 atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt gatcacaaaa
    40741 gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt gacggtagaa
    40801 acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat caaagacttt
    40861 gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt ggaaactgtc
    40921 ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa agaagtactg
    40981 cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc tttagctgga
    41041 aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta tcatatttgg
    41101 aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat caccgggagc
    41161 cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag gttctgggtc
    41221 tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt taaaagctaa
    41281 aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg cgacaaatga
    41341 agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag caatcgaaca
    41401 agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat atgtcatgag
    41461 tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag taacaaaagt
    41521 agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa gccaaaaagt
    41581 tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag cttctggtga
    41641 tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta ctgaccatac
    41701 cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg ggccatcagc
    41761 gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact tgcgacatgg
    41821 taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc gaatcggagg
    41881 aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct ttgcaccaga
    41941 tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca actacgaaga
    42001 agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag taaaaacagt
    42061 acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta aataggggtt
    42121 gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa atcttcacga taaaaataaa
    42181 aacaaaagaa aaaattgacg taattagatg ttgaagggac gattttgcca gagctcactg
    42241 tttcttcaat tttaaatgaa aggagtaatg aatgattttt actggtcatt ctttgaaaaa
    42301 agaacaatga tagaaaaaat cagcaagaaa atactagctt cacccaaatt gatcatttgg
    42361 atgtctatga tagttcttat cttttgtatc gcaagtggtt ttttattatc aaatttgcta
    42421 ttttatagag aaacagaaga gactgctgca aaagaaatac tttcttcgtt tagttccagt
    42481 ggagaagatc catcagctga aggaaacgat gtagaagagg atgctcgtcc aaaggtcatg
    42541 tacaccgata tcaaaggaag tgtgaaggag cctggtatct attccttttc ttcagaagaa
    42601 cgggtctatg atgtactaaa gcgtgcaggt ggattattag aagaagcgga tagcgatcga
    42661 atcaactttt ctgcgaagat agaagatcag caagtcctat atatccctgc tgtaggtgaa
    42721 gagctgccag aacatcttga tcagttggca tcctcggaag gaaaacagcc tacagcagat
    42781 tccgagcctc ccaaaataaa tatcaataca gccactccat cagaacttca gcagattcct
    42841 gggatcggat cagtcaaagc gcaagaaatc attcgattta gagaagaaaa cggatccttc
    42901 cagaaagtgg aagacctgca ggagatttct ggtatcggtg aaaagacagt tgaaaaacta
    42961 aaaaacttcg ttacaattaa atagaatgat tcaaaatcga ctggcacgag caataaatgg
    43021 acgctaatgg atactaagaa taatccaatt ctaagagctt ggcaggacga ggaggtagta
    43081 tttttggata ggaaatagct tgattgttcg caaagaaaca ttaaaaaaac caatgctgaa
    43141 tgtctatctc caaaataaaa tatctgggat tcatattatg aatacggctg tcagcggcaa
    43201 taacagtcaa gctctacgag aacgctttgc caaagatgta cttagctata cagcagataa
    43261 agtatttatt ctcattggga cgaatgatct agcagagcat aaacaacttt ctaaagaaac
    43321 gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa aagactatag ttctgatcat
    43381 gctttcttta tcacgccacc agcagtcgat gagacaaaac aaaaatatcg gaataacaag
    43441 ttgatcaaag aatatatctc tgttctcaca gaagtagcaa aacgccatga ttgtgaagta
    43501 tcgattttta tacggaattt cttgaacaaa tggattcgaa gaatttttat aatgggctaa
    43561 tggatgacgg acttcatttc ggcagtgaag ggtaggatct tttgtcagac aggatgattc
    43621 gttatattta aagaatattt tgtttaagca atgagtactt tttaatcaac ttgaaaaaac
    43681 ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa taaagaaaaa
    43741 gttactgata agtcacataa tgaacgcatt ccttgggacc agtactttat ggctcaggca
    43801 gtactgttat ctttacgtag tacgtgtaca cgtttagaag taggagcgac actcgttcga
    43861 gaaaaaagga tcattgctgg cggctataat ggagcagtca gtggagatgt ccactgcata
    43921 gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc agagatgaat
    43981 gcactcctac agtgtgcaaa acttggtatc ccgacggaag gttcagaaat ctatgtcaca
    44041 cattttccat gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa agggatcaat
    44101 tatctccatg actatcgaaa tgatccttat gcccaagcgt tgatcgaaca attaggtgta
    44161 tctgtacacc aagttgcttt agactcacag catttttcaa aacttcaaaa tgaattaaca
    44221 ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat cggttgattt ttccggcaat
    44281 tacgtcgatg attatctgtt tgttgtatta cactgacaat tggggttatc gtttgcttct
    44341 tgccggaatt tttttatggt tttcgctaaa agaaggacgt tttatcgcgt acttgtgtat
    44401 tttgtgtagc ataacggtat ccatttctta taggctacaa gagtattcat ttttagagga
    44461 aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa atggtaattt
    44521 tttgacaatg gaaggaaggt tatcagagaa taaacaaaag ctccagcttt cttatcaagt
    44581 aaacacagaa gcagaattga aaacatggct taacttccga ggaaagggaa tagaattgac
    44641 tgctgaaggc gagttcatct ctccagcaaa aaagcggaat ctttataatt ttgaccaaga
    44701 agcttacttc aggaaccata gaatttctgg acggttccaa attcaaaaga ttgaacagat
    44761 caccatgcaa aatcactggc gaaactggct tcagagaaaa agaggatctt ttatctcctt
    44821 tgtccaatct gaatttccgc aaaaaacaag tgtatatatt aattctttat tatggggcta
    44881 taaagatgca ggattcagag aaccagaaga aatttttagg gagagtggtt tgctgcatct
    44941 gttcagtcta tcgggtctac acatccaatt ttatttaggg tggctgtatt atttgtttcg
    45001 acgtatcgga tggccactac acgtcagtat tattccgttg agtctactaa ctatctgtta
    45061 tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt ttttattgcg
    45121 attgctagtc aaattgctga ataagcatta ttccaccatg gacttatttg cagttgtctt
    45181 ttggcttctt ttactatttg atcctcgatt tctttttcat gcgggtggac agcttagttt
    45241 tttcatgagt tttttgttta tcctgatttc ttttgatgga gattggttga aaaaaacggc
    45301 ctctcacgtc ctgtttacta tgatcacgat gccgttgatt gtatggcatt tttatgagtg
    45361 gcctatttta ggtagtcttt ttacattact gattgcaccg gtattcaagt ttctttttct
    45421 tcctggtgtg ttatttctca cactcttcaa tcgctggctg ccggaaactt tacttttgct
    45481 gatagaagag ggattaatct tatttgagcg aataatcgat ttttcttcag ggtcgtctct
    45541 cattatagga cgtttgcccc ttgtgctatc tgggatgtat attttcggat tactgatttg
    45601 tatggatagg ttgaaagaac aacctgtgaa acacttgttt tatgttggtc ttttttcttt
    45661 tatttttttg actttgccat ttttacgctt ttcttcaacg atcgctttta tcgatgtcgg
    45721 tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt tgatcgatac
    45781 tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac gtcctcctgc
    45841 tgaaagaaat atcgttccat atctaaaagg tcaggggata agtgtaatcg ataaactcat
    45901 cttgacacac gacgatacgg atcatgtggg agaattaccc acgttatctc agcattttac
    45961 tatcaaaaaa atttatatcg gttggggcgc tggacgaaat gagccattac aaaggcattt
    46021 atctgaagct caaaaaaatg gtactgagat cattgaaatc aaacatgggg acaggatcag
    46081 cggttattat gatctttatg tattgactcc ttttgaaaag ggagagggaa gaaatgaaga
    46141 ttcgattggt ctatggatgg aatataacaa taggcgcttt ttatttttag gagatttgaa
    46201 tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg atgtagtcaa
    46261 attaggtcat cacggtagcc gtacgtcaag taacacagat tttctttccc atattgaagc
    46321 aaaacatggg attatatcct gcggcgtaaa taatcgttat ggtcacccgc acagcgaagt
    46381 attggagaca ctagaagaaa atcagataaa aacatggcga acggatcaac aaggaatgat
    46441 cacctatcgc tggcatccag tttttcatcc tcatgggcta gtggaaacaa tgatagacta
    46501 gtaattctca tctgctcatg gtaggataat ttttgaaagt ggtgagtaat ttgagtatac
    46561 aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg attttaggaa
    46621 cagaaaagtt tctccaagat caagttcgga cagaaattgt caaaaaaatc caattagagg
    46681 gcgaagatga cttaaatttt ttaagttttg acatggaaaa tgcaagttta gatgaggtag
    46741 tagctgaagc agagacgttg ccgtttttcg gagatcaacg gttagtattc gtagagaatc
    46801 cttatttttt aacaggtgaa aaagtaaaca atggaatcga acaaaatact gatttattga
    46861 cggcttattt gaaagaacca ttagaatcga ctgtattagt attctttgcg ccatatgaaa
    46921 agttggatga acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt atcatcaatg
    46981 tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat gaatacctta gagaattcct
    47041 caatcaaaat ggaacgaagt gcaattgatt tattcttgag gttgaccgac ttagatttat
    47101 ccaaaatgat gagagagtta cagaaattgg tcctttatgg acaaaatcaa cagcaaatca
    47161 cggtaagaga agtagaagaa ttagttccta aaacgttaga acataatatt tttgacatga
    47221 cccaatatat tttaaaaggg aagacggaac aagctttgcg tttatacgaa gatttagtta
    47281 tgcaagggga agaaacaatc aaaattaatg cgattttgct ttctcaatta cgtttatttt
    47341 tacagacaaa atttcttgta aagattggtt atcagcaggc aaatattgct gaaacattga
    47401 aaatccatcc atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc gatgaacatt
    47461 tgctggtaca gttgtttgat caattagttg agatggatta tcaaatcaaa acgggacaaa
    47521 tcgaaaaaga attaagtttt caactattcg ttttgcgcac agggcagttg ttaagaaatc
    47581 gataagaaga aagacgaggg caaagcatgc gggaaggtcg tttgtttttg tccttttttt
    47641 gacaaataaa aaaatcggcc gaagccgatt cttttattta gcgatttttt tgcttaaacg
    47701 agatttgtcg cggttagctt tgtttttgtg gattagacct tttgattcag ccatatcaat
    47761 agctttaaca gcttctttat ataacgcatc tacgttgtca gcacctgaag ctacagcatc
    47821 ttcaaatttt ttgattgcag tacgcatagc gcttgtttga gatgaatttt tcacattagc
    47881 gttttcgctt gtacgaacac gtttgattgc agattcaatg tttggcatta gtttcacctc
    47941 cgataactta agttcttacg cgtgttaccg cataattcaa cattcattat tatacctaaa
    48001 ggcagcgtgc tttgcaatat gaacatgaca agtttttttc cttaacagtc ttttattagg
    48061 attttttttc agtaaaaacg gatagtacaa gctgaaaaaa atcaaaaaga cgctattgtg
    48121 ggaggcaata gcgtcaaacc aaactagatt tttatttatc tatttaaaca aagtttgaat
    48181 gggaatgttt ggtactaagt tattatataa caaagagaac cgtttgcaaa gttttttttg
    48241 aaaagggata cgattccctg ttgttctttg tttttctatt aagaaactgc agttatttta
    48301 taaccagtat cctttgatca gtaaaggcta ttctggtgta ccgattattt taatgtattc
    48361 atcgatattt tttatcaaca ttttttttat gaccaactgg ttccgacggt tccatttgcc
    48421 acttacttcc agcacatatt tatttagggg caaacgtaag atatattttg ccaaatagcg
    48481 atctttaagc aggccattga tcacacggtc ctcaagctgc aagttgatcc cagtcagcat
    48541 cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag tttccacaag
    48601 cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact aattcttcca ttttgatatt
    48661 cgttgaagtg gatgcgatca ttctttcttt tgtccactgt aatctagtat aacaaaaaag
    48721 agcagaagag ccaaaaagaa aaaagagcat cgaattctat aggaagtctc attggcacgg
    48781 cctaaattat tacaaaacat tgattaaaaa aagcccctgc atcgcaggag catcccctat
    48841 atcgttttaa taggtcaatc aaaaagcagt atcaaacaca ttttgtatca aatcaggaaa
    48901 taatagataa gctaagccca gaatcaaaag taccgctaga agaaaccagc cgattgttcc
    48961 tatgaaatac tttaattttt cgaacattgt atcacctcgt tgtcagtata tcacggatca
    49021 gtaaattggg ggtgttaaga atctcctaaa atagacaaaa tttgttctat tttcctaaag
    49081 caaatacttg taagatacag taagtgatac cagaagcaat gaccggtccg gcggcgattc
    49141 ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg atttgtggat
    49201 cagaagcaat caatcctaca cccttggaag ataagacagc cactagccca ccgcaaaaaa
    49261 cagctaccca gccaataggg gatttaaagg cttctaatag atcacgtaga ccgatttctc
    49321 ctgttgcgat agggattaga atagccgctg tgatgatcgt caccccaata ttgattcctt
    49381 tgccttgaag ccaagggaaa aaggaacggg aggctggtat gagcttcata atcaaaacaa
    49441 aagctgccgc tatcatcaga ctttgatttt tagctaaaat tgctacaagt aaaataattc
    49501 ctaaaaaaag ccaactttcc atgaaatata ttcctcactt tcttcgaaac aatcataaca
    49561 tgtttttaga aagttacaaa agtaggaatt catccttcaa gacctattca taagctgttt
    49621 ttttcataaa ataaacaaaa tcataaaaaa acactttgct tatttgacag aagacagccg
    49681 cgctttgtat agtggacaag acttacattt ttttgaagaa tcagaaagat caggtaaagg
    49741 tcttagtaaa cgcaagccat tcagaataac caatattgtg cttccttcgt gtcctacaac
    49801 tccaagaggt agatttaaaa attgcaggaa gttagaagca atcaacaaga gaataacaag
    49861 aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt tttaaagata gccgatgact
    49921 gtagacaaat ttatttaagt cgcttttcat taagacgatg tctgcgatat ccatagcggc
    49981 atctgtccct tgccccatag cgacacctaa agaagcggct gctaaagcag gcgcatcatt
    50041 gactccatca ccaaccatcg catttacttg gtaaagttca ttctcttttt tgactagttg
    50101 tgttttttct tccggagtac agcttgcata gtaatgttca atccctactt gttcagcaat
    50161 ggcctttgct gtgccagaat ggtcgccggt tatcatactt gtatggatgt tttgggattt
    50221 aaagtaggaa attacttgtt gggtatttgc ttttggaata tctagcagac ctaaaacagc
    50281 aattaactgc tgatctttag aaagataaat cacggttttg ccttgatttt ccaaacgttc
    50341 gatcatttcc gcaatctctt tgctgattat catcgtctca ggatcatatg cgtgcttgcc
    50401 aactttccat tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa tttcttctat
    50461 tggtaagtat tggatttctt caggtattgt gcaatcgaaa tattgcaaaa tcgcatgagc
    50521 aagtggatgc gtcgtttttt gttccatagc aacaagaagt tgttgtgcca aatccttttc
    50581 atcaaagaaa aagctgtcag ttacaactgg ttttccttta gtgagcgttc ctgttttatc
    50641 aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg ataccgcctt tgattaaaat
    50701 cccatttttt gcagaatttg aaagagcagc tagagaagct ggtgtagcag atgcgacaag
    50761 cgcacaagga gaagccacaa ctaataatac cattcctcga taaaagcttt catcccaact
    50821 ccaacctagg aaaaagtggg ggagaaggat cattagagga acagcaagca aaatcaattt
    50881 tacataatta ttttcgattc gttcgataaa tgaagctgtt tgagttggcg tattctgcgc
    50941 ttcttctact aattggataa tcttagcaaa gacagtttcg gaactcgttt ttgttactcg
    51001 aatcgtgatg ggatttcctt gattcagcgt tcctgcatac atctcatcgc ctaaatattt
    51061 ttctacaggc acagattcac cgcttaatgc agactcatcg atcgttgcct gtgtgctttc
    51121 taaaaagcca tctatcggaa cagctgctcc tttagcgaca aataccaagt tatccacctt
    51181 tagttgagaa acatctactt cttgcatttg tccattttct tttaataatt gggctttttg
    51241 tggttgaaga ttcatcaaag cagtaatttc tttttgactt ttattagttg tgtattcctc
    51301 taatgcaccg cttaaacaaa agatgaatgt caacattgct ccttcaaagt aattaccgat
    51361 caaacaggca ccaatagcag ctaaagccat caataaatcc acattcagtg tccgatcatt
    51421 ccacaattct ttcaagcctt ccgatgtttg tttatatcca ccgatgataa tggcactgat
    51481 ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag ataaaaccga agatcataaa
    51541 taaaaggcag ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg ttattagacc
    51601 tccttgtcaa aacaaggata gcataataaa aatgtaattg ataataataa cagctaaatg
    51661 agaacgataa tggttatcat ttggtgatta agcttgatgt tgtttttatt atttataatg
    51721 attattatta aagaaaataa aaaaccagaa catttctcaa ataagagaag tgttctggtt
    51781 ttttctacat tgcaataaac caataaccaa tcaaaatgat accaaggata atacgatacc
    51841 agccaaaagc ggtaaagtcg tttttcttca aatagcttag taagaatttg atagctaaga
    51901 cggaaaccac aaaagatacg aatgttccta caagaagaac aacagtttct tccattttga
    51961 atgaattccc ttcagctaaa tatttgacga tcttcaagcc gcttgcgcca aacatgacag
    52021 gaatccctaa gaaaaatgaa aactctgtag cgacaaaacg cgaagcacca atcaagatag
    52081 atcctaggat cgttgcgcca gaacgggatg ttccgggaac aagtgccaag acttggaata
    52141 acccaacaag aagagctgct tggtacgtaa agtcattcag agaagtccaa cgaggtgttt
    52201 tgtttttgtt gcgtttttcg acaataataa aggcaatacc gtaaacgatc aaaacaatgg
    52261 caactggtaa gaacttgtgg aaatttgctt ctaaccaatc atccaaaggc aaaccaatga
    52321 tgatggctgg tatgcaagca acgatcactt taaaccaaag tgtccacgta tctttttttt
    52381 ctaccgttga tttacttgga gaaaatgggt ttaacttgtg aaaataaaga acgacgacag
    52441 ccaagatcgc ccctaattgg ataacgacat tgaacatcga cataaattgt gaactggcat
    52501 ccagtttgat gaattcatct gctaggatca aatgaccagt actactgatg ggcagccatt
    52561 ccgttattcc ttcaataata ccgagaatca ctgctttgat aatatttgca aaaaacataa
    52621 attccttcct tcccaattaa gatacatctt atagtatacc gttatttttt gagaaaccca
    52681 atctgttttt taatattaag aaaaacttga gaaataaagc agataagtac acgaagagaa
    52741 aaaagaagac aaagccctcg ttcagacttt gccttttatg attctctggt aaaaattaaa
    52801 atatccccat tatagaaacg agtcgcgact atgtgttttc ccgttatcga tataagacgt
    52861 atcattgatg ccgtggatag aaaatcgacc gtttgcataa gtgacctttg ttacgctgcc
    52921 atttaaaagc ccagcacgaa caggctgttt aggatcgatc agattaagca aaaaggcgat
    52981 cgtcaaacca tgagatacga ctaaaacatt tccaccacca tttttttcgc agtgatgggc
    53041 gatatcttca aatcctgtcc atacacggtc tcgcaatgtt tcataagttt ccgcccaccc
    53101 agcggtatct aagttgtata cagcattagc aatttgttca aaagaaactt ctgtggtaaa
    53161 catttcatca tatgttttaa aattcaatac acgagggatc acaccccaca tttccatgtc
    53221 atatccacct tcaagagacc cgaaacacca ttctctaatc cttttttcaa taaaataagg
    53281 aatttctttt cccttagaat gttctcctag aataatacgg gcagtttcga ttgcacgacc
    53341 actgtcacta gaataagcaa atgaaaagtc gacatttttt aacccgcgac ctaaataatg
    53401 aatgccttct tgtccttgct ttgtgagggg tgtatcacac cagccttgta ctcgttcaat
    53461 tgtattgaac atcgtttttc cgtgtcgtac gatataaagt gtagtttctg tcattttatt
    53521 ctcctcctaa gtcgttttaa cgattgaaaa aaggatttgt ccttttttcc tgttcaatcg
    53581 tcgttgcatc gccatgtcct ggatagactg caaattcatc aggtaataca aacagctggg
    53641 tccgaatact atgaagtaat tgttctaaat tccctgtata cagatcggtt ctaccgatac
    53701 ttcctctaaa cagggcgtcg ccagaaacga caaaatcatc aaaaataaag ctgacgcttc
    53761 ctatagaatg ccctggggta gggacgacgc gaaaagacat gtttcctaaa gtataagttt
    53821 tcatttcaaa ttcatattct gcaggcgaga caataacatc agcaatatca tcatgtctac
    53881 cgagtccaga aagattcaaa atcggatcac ctagccattt ttgctctagt gggctgacat
    53941 aaactgggat ttgataatac gcgcgtaaag gttccactgc accgatatga tcgtagtgag
    54001 tatgggtaag caaaatcgct accggctgtt ggtttgtttt tttgatttct tgtttgattc
    54061 gttccgcatc ttctccaggg tcaatgatca aaagtgcttc ttcgttgtag accaaataac
    54121 agttttcttc aatcattcct gtttttaatc tttcgatatg catgtgcatt cctccttgat
    54181 agccaaaact tctttcatcc attagtataa cgcattcatt gctaggaaac aaaaacaggt
    54241 acttatttct ctttacttat ctgaattatc agactatatg aaaagtattt ggatattcta
    54301 aagagaagga aagtaagttt tcatagcatt ttaatatgtc cttgttacat tagattcatc
    54361 caaaaatcca aattctatat cctatatccc tgcctaatca gcagggtgtt tttttatgtt
    54421 tggaaagaga gctctttttg ctaaactagg ttttttcagg taatcttaga gagaaagagg
    54481 tggtggaaat gatcaaacag tcaaaaatat ctgtgcggca cttagtagaa tttattttgc
    54541 gacgtggaag tatcgacaat cgtaaaaaaa gtaaccatac tgcattagaa ggagcgagaa
    54601 tccaccgcaa gctccaaaaa gatgcgggag aaaactatga aaaagaagtc tttttaaaaa
    54661 caacagttca attagatgaa tattacctga tcgttgaagg ccgtgcagac ggtattttca
    54721 aaagagacgg tatttattat atagatgaaa tcaaaacctc cgaaccacga tttgaagatt
    54781 tagaaccaga acaagtggaa cttttctttc atcaagcaag ggtgtatgcc tatatctata
    54841 gtcaagaaca tgatttacaa gaaataaatc tgcaattgac ttacttccag acggcagaag
    54901 aagtgatcac gagaaaggtt gaacatcaga caagagagca gttagaaact ttttttaaaa
    54961 agctgactga agattataaa gaatggcttg tttttcaaga aaactggcgc actgtacgca
    55021 atgcctcttt gatggctttg aaattccccc atgatgagta taggaaaggt cagcgtgaac
    55081 tagcggtagc tgcttataaa acgatccgta cgaaacagaa attgtttgta gaagcaccta
    55141 ccggaacagg aaaaacaatc tctacgttat tccccgcttt gaaagctgta ggggaagaag
    55201 agggagaaaa gatcttttat ctaacggcta aaacaataac tagacaagta gctgaagatg
    55261 ctatgacagc attaaaagat acaggagctg aagtaaaaag tgtcactttg acggcgaaag
    55321 acaagatctg ctttttagat gagacgacct gtaatccaga tcagtgccca tatgccaatg
    55381 gttattacaa tcggattaat gaaggtttat gggatctttt gaatcatgag aatcagatca
    55441 ctcgtgaagt catcgaaact tatgcaagaa agcatacact ttgtcctttc gaactatcat
    55501 tagatgtcag tatctggtgt gacgtaatca ttggggatta caattattta tttgatccaa
    55561 ctgtttattt aagacgtttt tttgaagatg aaaaaaacga ggactatctg tttttgattg
    55621 acgaagcaca taatttggtg aatcgttccc gagagatgta ttcggcagaa ctatcttatg
    55681 aaaaaacgaa acgaagtaag gaagctatac caaaggaatt caaaaaactt catcgccgtt
    55741 tcaacaaact attaaaagaa tttgattcga tccgagaaat agcaaaagaa gatcattggg
    55801 attatcatca tcaaaaagca ccggctgaat ctttagtaaa ggcaggttat caattaagcg
    55861 aaaaaattaa agaatggctg gcagaatttc ctgagcaccc atcacaagaa caattgcttc
    55921 cttattattt tgatttgctt cattttttga aggtcagtga attttatgat gatcactacg
    55981 aaacaacagt cgaaaaaacg tatcgcgatc tgatagtaaa agagttttgt attgatccaa
    56041 gcttgtttct tgagcagtcg ttggataaag gaaaaagcag tattcttttt tctgctagtt
    56101 tttcaccact ctcttattat caggaaacac tggggggaca aaaaagtctg gcttacaaac
    56161 tgccgagtcc tttcccagaa ggaaaccagc aagttttcat cgcaaattac ctagagacga
    56221 cttatcgaaa aagggaacac agtctggcaa aattagtaga gacgattcat gcttttgctg
    56281 atggaaaggt cgggaattat ttcgttttct ttccttctta tcaatattta gatcttgcgg
    56341 tggaagcatt cagacagcaa tatcccgaca gcaatgtgct aatccaagaa acggacatga
    56401 atgaggaaga acgcgaacga tttttaacaa aatttcaaac aaatcccgct gaaacattga
    56461 ttggattttg cgtacttggc gggatttttt cagaaggaat cgatttgaga ggggaccgat
    56521 tgatcggcag tatgatcgta ggtgtaggtt tgccgcagat gaatcatgag caggaactga
    56581 tcaaatcata ttatgatgag aaggaacacc ttggatttgc ttacgcttat cagcttcctg
    56641 gaatgaacaa agtcttgcaa gctggtggtc gagtgattcg tggcatggcg gatcaaggaa
    56701 tcgttgtatt ggcagatcaa cgattttcta gtttttctta tcgacgttta ttcccacaac
    56761 attggcaaac agcaagagaa gtacgaagtg ttggggaatt ggaagaagga atacagcgtt
    56821 tttggttatc aaaagaagca gtaaatcaaa aaaacgagga atgacagacg tcagcctcgt
    56881 tttttgatta acgatagtta gtgaattgca actcgattgg taagtcaagt ttacgtaata
    56941 aagcaatcac agcttgtaga tcatcacggt tcttacctgt cactctaatc tgatcttctt
    57001 ggatttgtgc ttttactttc agtttttcat ttttgatagc tgtagtgatt ttcttcgcgt
    57061 tttcacgatc gattccgttg accaaatcgg cagtttgtcg agctttgcca cctagagcgt
    57121 gttctgattc ggaaaaatgg atatttttga ttggcacatt tcttttgatc agcttaccaa
    57181 ataatacatc tttgatctgt tcgattttga aatcatcatc gctcaataca actagattgc
    57241 tgttctctaa tttgatttct actgtagaat ccttgaagtc aaagcgattg gacaattctt
    57301 ttgttgtttg ctgaatcgca ttttttactt cttctgtatt gacttctgat acaatgtcga
    57361 agctagcatc ttttgccata ggaaccctcc tgatatacat ataaatattt accatacatc
    57421 tatcttatca taggattagg gcagattctt acctgtttgc taaaattctt ctcatttgag
    57481 ttcgaagtat tttattttaa tagataaact gttattctta catttggaat accaaagtag
    57541 gggagaggtg cttgtgagta aaattatgac tgcgtatcat aaaaatgaaa gattaaagaa
    57601 aatagtggtt attttgataa ccggattgac cgctgcggtc gctttaaatt attttttgat
    57661 tccagccaat gtcttttctg cagggatgaa tgggatcgct caaatcattt cctcgctttt
    57721 atatgaatgg tttcatatca agatagacac aggaacattt atttttcttt tgaatatccc
    57781 tgtgtttgta ttagggtttc tcaaagtagg aaaacgagca acgatcctta gttttgtgaa
    57841 tgttgtatgt gtatcggtga tgacgatgct gcttccagtc ggacagatca ctgataatat
    57901 tttgatgaat gccctagtag gcggtgtttt attaggtctt ggcgtaggat tatctttaaa
    57961 aatgggcttt acaactggtg ggatggatat catctctctt gtactttcac aaacaactgg
    58021 aaaaacagtc ggaaactaca tgctgattct aaatggtatc attgtagtca tcgcaggctt
    58081 cttctttaac tgggaaagtg ccttgtatac gattatttcc atttatgcaa tgactcaagt
    58141 aattgatgcc atccacacga gtcatcaaaa agtaacagca atgatcgtta ccgtcaagcc
    58201 agaagaggta acagaagaac tagcaaaacg gatggtacgt ggaatgacat tactgccgtc
    58261 tgtcggtggc tattcaaaaa aacaagggaa catgatcatg atggtcatca cccgctacga
    58321 aatgtatgat cttgatcaga tcgttcatga aatcgacgac gatgcattta tcaatatttt
    58381 accaactcat tcagtatttg gccggtttgc aaatgaaaac gaacaaaaaa tgtatcgaac
    58441 aactggcgta ttgccagaaa taaaaaatcg ttcacaaaaa aaatgaggaa tcaggtttat
    58501 tttagaataa atttaatcta ttacatctaa taagaacatt tttataaatt taaacgagaa
    58561 tcttacttct aaattctcat ttacaagcag ctaaaaaaag agtatgataa ataaaaatcg
    58621 tgttaaggag tgtcgtacga tgagcgaagc aaaccaagaa cggtctggac aagaaataga
    58681 actaatcaaa gaacgtattc ttgctgcctt ggaaatggtg attgatcctg aattaggaat
    58741 cgacatcgtc aatttaggct tgatttatga tatagaattc aatcctgaaa atggagaaac
    58801 agtcattaag atgactctaa caactatggg ctgtccatta gcagatattt taacagaatc
    58861 tatccatgac gcattaaaag aagtgccaga agtaacgaaa gcagaagtca aactagtctg
    58921 gtatccagca tggacgacag ataagatgag ccgttatgca cggattgcgt tggggatacg
    58981 atagaaatct tggctgacta gctatgagaa gcaaatagga aaaacaaaaa agccttttat
    59041 cctttctaga agaaaagaga taaaaggttt ctttgtttat aaaattttgt ctgctatatt
    59101 ataagttaat taaataaatg tgttctattg ataaaggaca ttcaaatccc tttccttttg
    59161 gaatccttct tttttacatc agtttgattt tcgtatagta ttctttctca tcttcacgat
    59221 aaatcgcagc cccatttttt tgttgtgctt taagactgcc aagatttttt ttatttacag
    59281 acaaaagtgc ctcatcaatg ccgatttttt ttgcctcttg tagtactaat gctaatcctt
    59341 gtgtggcata tcctttttta cgataattag gggagatgcc gtaaccaata tggccagcgc
    59401 cgtttcttaa tgtttcgttc agtttatggc gcaaattaaa aattcctaca tactcatgtt
    59461 catcactttc taaaataaag acggttgcgg gaacgtaact ctcaggaaga tcgattccta
    59521 aagacgtttt ttcgcatttt tttacataat ctaaaaattc agcaaatgta tagccataag
    59581 cattgttgat aaatccactc tcatttggtc caaagctagt ttgtagctga taagctttct
    59641 ttaaatctgc ttcccatagt ttgatcagtt tcatcgtatt cacctcttta agtaacctta
    59701 ttcatttgtt ttctactata gcactattgt tgtaaatagc catgcctatt tttatttatt
    59761 gtgtcatatc gtattaagaa tcatcgaaca atacaaatat gaattacaat aaaaaaatta
    59821 tttgaagttc gttcattcgt tacttatgat ctctagaaga aagaaaacgt gaattacttc
    59881 agcgtctaga attccagaag gatagtaatc aacagaagaa ataatcaata tccaaatgaa
    59941 agaagatagc tgaatcgaga aaaagaggaa aaaagaaatg aagaaaaaaa taaaatagtt
    60001 actaatccca gagtcgttcg tttaacattg aaatcgatca tatggataaa acagattttt
    60061 tcaccaaccc tcaatagatg atggagttgc tcggatgaat tatttgtgta gatggcataa
    60121 gggagggcgt acagaaatga aaaagaacaa aaatatgttc ttgacaaaaa acacatgtaa
    60181 tcctaaacta aaagatgtga tgcggctatg gcggaattgg cagacgcgct agcttcaggc
    60241 gctagtgagg gtgacctcgt ggaagttcga gtcttcttag ccgcataatt tgttttttta
    60301 taaagagaag gtacacctgg agcgatgaga aagaaaataa catattatct aaaagaatat
    60361 aggaaaaatt ttaaacaacg gaaccagatt caaataaatc aaaaaaaatt tgtgtgaagg
    60421 tgggacagtt taaaacctaa tagattgatt caaatagtag atttatagat tctgatacaa
    60481 gaaatactgg caaacgatag ttaaggaata aagactaaac ttaaactcaa aaagaggtca
    60541 tgcgaaagaa taaatcgcat gacctcttac tatattttga atgtgtttaa acgttcaaa
//
LOCUS       DASJUF010000005        50074 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJUF010000005 DASJUF010000000
VERSION     DASJUF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.005"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..265)
                     /locus_tag="U4B62_000272"
     CDS             complement(<1..265)
                     /locus_tag="U4B62_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2140460.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAG"
     gene            complement(267..464)
                     /locus_tag="U4B62_000273"
     CDS             complement(267..464)
                     /locus_tag="U4B62_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN2140461.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(858..2585)
                     /locus_tag="U4B62_000274"
     CDS             complement(858..2585)
                     /locus_tag="U4B62_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN2140462.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2578..3771)
                     /locus_tag="U4B62_000275"
     CDS             complement(2578..3771)
                     /locus_tag="U4B62_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2140463.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3958..4602)
                     /locus_tag="U4B62_000276"
     CDS             complement(3958..4602)
                     /locus_tag="U4B62_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2140464.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4696..4830)
                     /locus_tag="U4B62_000277"
     CDS             complement(4696..4830)
                     /locus_tag="U4B62_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN2140465.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4832..6964)
                     /gene="feoB"
                     /locus_tag="U4B62_000278"
     CDS             complement(4832..6964)
                     /gene="feoB"
                     /locus_tag="U4B62_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN2140466.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6961..7434)
                     /locus_tag="U4B62_000279"
     CDS             complement(6961..7434)
                     /locus_tag="U4B62_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN2140467.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7835..8059
                     /gene="nrdH"
                     /locus_tag="U4B62_000280"
     CDS             7835..8059
                     /gene="nrdH"
                     /locus_tag="U4B62_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN2140468.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8218..10377
                     /gene="nrdE"
                     /locus_tag="U4B62_000281"
     CDS             8218..10377
                     /gene="nrdE"
                     /locus_tag="U4B62_000281"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN2140469.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10427..11392
                     /gene="nrdF"
                     /locus_tag="U4B62_000282"
     CDS             10427..11392
                     /gene="nrdF"
                     /locus_tag="U4B62_000282"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN2140470.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11481..12620)
                     /locus_tag="U4B62_000283"
     CDS             complement(11481..12620)
                     /locus_tag="U4B62_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2140471.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12929..13642)
                     /locus_tag="U4B62_000284"
     CDS             complement(12929..13642)
                     /locus_tag="U4B62_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN2140472.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13896..14597)
                     /gene="nagB"
                     /locus_tag="U4B62_000285"
     CDS             complement(13896..14597)
                     /gene="nagB"
                     /locus_tag="U4B62_000285"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN2140473.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14831..16363
                     /locus_tag="U4B62_000286"
     CDS             14831..16363
                     /locus_tag="U4B62_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2140474.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16554..17756)
                     /locus_tag="U4B62_000287"
     CDS             complement(16554..17756)
                     /locus_tag="U4B62_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2140475.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17838..18527)
                     /locus_tag="U4B62_000288"
     CDS             complement(17838..18527)
                     /locus_tag="U4B62_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2140476.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18633..19001)
                     /gene="rplL"
                     /locus_tag="U4B62_000289"
     CDS             complement(18633..19001)
                     /gene="rplL"
                     /locus_tag="U4B62_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN2140477.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19054..19557)
                     /locus_tag="U4B62_000290"
     CDS             complement(19054..19557)
                     /locus_tag="U4B62_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN2140478.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19761..19888)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19967..20656)
                     /gene="rplA"
                     /locus_tag="U4B62_000291"
     CDS             complement(19967..20656)
                     /gene="rplA"
                     /locus_tag="U4B62_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN2140479.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20760..21182)
                     /gene="rplK"
                     /locus_tag="U4B62_000292"
     CDS             complement(20760..21182)
                     /gene="rplK"
                     /locus_tag="U4B62_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN2140480.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21525..22193
                     /gene="sdaAB"
                     /locus_tag="U4B62_000293"
     CDS             21525..22193
                     /gene="sdaAB"
                     /locus_tag="U4B62_000293"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN2140481.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22206..23078
                     /gene="sdaAA"
                     /locus_tag="U4B62_000294"
     CDS             22206..23078
                     /gene="sdaAA"
                     /locus_tag="U4B62_000294"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN2140482.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23169..24188)
                     /locus_tag="U4B62_000295"
     CDS             complement(23169..24188)
                     /locus_tag="U4B62_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2140483.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(24361..25266)
                     /locus_tag="U4B62_000296"
     CDS             complement(24361..25266)
                     /locus_tag="U4B62_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2140484.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25325..25870)
                     /gene="nusG"
                     /locus_tag="U4B62_000297"
     CDS             complement(25325..25870)
                     /gene="nusG"
                     /locus_tag="U4B62_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN2140485.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25971..26141)
                     /gene="secE"
                     /locus_tag="U4B62_000298"
     CDS             complement(25971..26141)
                     /gene="secE"
                     /locus_tag="U4B62_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN2140486.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26165..26317)
                     /gene="rpmG"
                     /locus_tag="U4B62_000299"
     CDS             complement(26165..26317)
                     /gene="rpmG"
                     /locus_tag="U4B62_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2140487.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26479..27123
                     /locus_tag="U4B62_000300"
     CDS             26479..27123
                     /locus_tag="U4B62_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2140488.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27179..27682)
                     /locus_tag="U4B62_000301"
     CDS             complement(27179..27682)
                     /locus_tag="U4B62_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2140489.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27695..27741)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28279..29244
                     /locus_tag="U4B62_000302"
     CDS             28279..29244
                     /locus_tag="U4B62_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2140490.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29343..30515)
                     /locus_tag="U4B62_000303"
     CDS             complement(29343..30515)
                     /locus_tag="U4B62_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140491.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30502..31647)
                     /locus_tag="U4B62_000304"
     CDS             complement(30502..31647)
                     /locus_tag="U4B62_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140492.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31661..32585)
                     /locus_tag="U4B62_000305"
                     /pseudo
     CDS             complement(31661..32585)
                     /locus_tag="U4B62_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32702..33781
                     /locus_tag="U4B62_000306"
     CDS             32702..33781
                     /locus_tag="U4B62_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2140493.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33813..34406
                     /locus_tag="U4B62_000307"
     CDS             33813..34406
                     /locus_tag="U4B62_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2140494.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34516..34743)
                     /locus_tag="U4B62_000308"
     CDS             complement(34516..34743)
                     /locus_tag="U4B62_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2140495.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34846..37434)
                     /locus_tag="U4B62_000309"
     CDS             complement(34846..37434)
                     /locus_tag="U4B62_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN2140496.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37454..38113)
                     /locus_tag="U4B62_000310"
     CDS             complement(37454..38113)
                     /locus_tag="U4B62_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2140497.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38162..38665)
                     /gene="trmL"
                     /locus_tag="U4B62_000311"
     CDS             complement(38162..38665)
                     /gene="trmL"
                     /locus_tag="U4B62_000311"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN2140498.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38886..39752)
                     /locus_tag="U4B62_000312"
     CDS             complement(38886..39752)
                     /locus_tag="U4B62_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2140499.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39861..40502
                     /locus_tag="U4B62_000313"
     CDS             39861..40502
                     /locus_tag="U4B62_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN2140500.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40564..41925)
                     /gene="mgtE"
                     /locus_tag="U4B62_000314"
     CDS             complement(40564..41925)
                     /gene="mgtE"
                     /locus_tag="U4B62_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN2140501.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41943..42842)
                     /locus_tag="U4B62_000315"
     CDS             complement(41943..42842)
                     /locus_tag="U4B62_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2140502.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42844..43641)
                     /locus_tag="U4B62_000316"
     CDS             complement(42844..43641)
                     /locus_tag="U4B62_000316"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN2140503.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43619..44323)
                     /locus_tag="U4B62_000317"
     CDS             complement(43619..44323)
                     /locus_tag="U4B62_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN2140504.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44454..45030
                     /locus_tag="U4B62_000318"
                     /pseudo
     CDS             44454..45030
                     /locus_tag="U4B62_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45148..45825
                     /locus_tag="U4B62_000319"
     CDS             45148..45825
                     /locus_tag="U4B62_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2140505.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45904..47712)
                     /gene="pepF"
                     /locus_tag="U4B62_000320"
     CDS             complement(45904..47712)
                     /gene="pepF"
                     /locus_tag="U4B62_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN2140506.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47888..48886)
                     /locus_tag="U4B62_000321"
     CDS             complement(47888..48886)
                     /locus_tag="U4B62_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN2140507.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49112..49765)
                     /locus_tag="U4B62_000322"
     CDS             complement(49112..49765)
                     /locus_tag="U4B62_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN2140508.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14992 a        10504 c         8779 g        15799 t
ORIGIN      
        1 ttccagcagt tcttccaatt tcactcaaat ttcttattaa ttcctcttga tattcagtga
       61 atcttatttt attagtataa gcagtaaaat atagataatt caccaatcta tttattaaag
      121 gaccgggatc taattcatat ttttgttttt ctaattttaa atataccttt tgtaaaacct
      181 caagaatatc agaatattta ggatctttac ttatagatat atcattatat aattcatgaa
      241 caatttgctt agcatttttt ttcattttag cacttccctg gaattgctcc acctaaaaaa
      301 ccacctatag acattcctgc aatacaagta gttgccttgg cccaatcgac cgtacattta
      361 tttttagtgc aatacactcc atttccataa tattttccac tatgagtggt acccccatag
      421 ataagttgtg tctctttaat agacaaaatt tttaaatgtt tcataatcta atcaccccta
      481 aattaatgtt tttagactat tattctaaca ttcattaaat ttcaaaaaac aattccttct
      541 tgaaacgtag caaaataatt cctgaataag aatttaatca cttttaagta tataaactat
      601 gtatattctt taatattttt tgagaatgca cttcgtacat aataaacttt aatcacatta
      661 gtctttcata cctacgatcg atctctttga ctcgcaccca taaattcata atagtttgtc
      721 ggcgtctttg gacgtttcga agatgagcta cgcattctct ttttttatag acaaaaaaga
      781 tagagacgaa catggagctt ccaaatttgg atatttgatc caattttgga agctccattt
      841 atttcgtgtc tgtcttttta ttttttccag ctttcgacta attcttggtt ctctttgatc
      901 cagtcttttg ctgcttgctg tggatctttt ccattgttga tttccaacat aactttttcc
      961 atatcttttt ctgaccaatg gaaattgtct aatactttgt aagcttctgg ctggtcttct
     1021 ttcaatcctt ttcgaaccat cgtatggatc tgctcttcgc ttcccattcc gttttcagga
     1081 tcttctaagt atttcagatc atatttggca aacatccaat gaggtgtcca tcctgtaact
     1141 acaattggtt cgtgtttttt gatcgcttga cctagtgcaa cggtcattgc tcctgaagaa
     1201 gatgtctcta ctttccagtc tttcaagttg tcatattttt ggatcgtgtt ttcagcagcc
     1261 gtaactacac cagctcctgg ctcgattcct atgattttct tacctgcctg atcggttaac
     1321 tcatcgatcg aatttacatc catgtaggct ggcactgcta aaccaacttt tgctcctgtc
     1381 aaattggcac ctaggtcttc gacttgatct ttatattgtg catattgtga accatgagtt
     1441 ttaggtagcc aagctgaaac catggcatct gactcgccat ttgaaacaga tttccacatg
     1501 attgaattat ctaatggcgt catcgttaca tcatagccca tttgtttcaa tacttcaccg
     1561 actacatttg ttgaagcaac ttctgtatcc cattcaacat aagataagtt gatacgtttt
     1621 gtttctttgg cagatgaaaa actccctata ccaatcgcac ctaagatcac tactactaca
     1681 gcaccaatga tcagtccttg acgtttttta cttttctgtg ttgttgctcc tgcagctttt
     1741 ttattgtttg gctggttcaa atgctgtgta aaacggtcaa tgatgattgc taagataact
     1801 aatgcaaccc cgttgacaaa tccattcccg acttgtgctc tttgcaatgc tgataatacg
     1861 ccgcgtccta agccgggtgc accgatcata gaggcaataa cgaccattga aagtgacagc
     1921 attgttgttt gattgatccc tgccatgatc gtacttttag ctaaaggcaa ttctagtttg
     1981 aatagttttt gacgacttgt gctcccgaat gaatcagaag cttctactaa ttctttagga
     2041 acttgtcgaa tccctaaatt cgtaaaacga acggttggtg gcagagcaaa gatgactgag
     2101 gcaaataccc ctggaaccat cccgataccg aaaaatgcaa ctgctggaat caaataaacg
     2161 aatcctggca ttgtctgcat aaagtcaagg atcggtttga taatactttg ggctttactg
     2221 ctttttgcca tcaagatccc taatggtacc ccaatgatga tcgagatcac actagataaa
     2281 agaacaagtg tgacagtatt catcaaatca ttccatagac cttggttata aataaaccat
     2341 agaccgatta atgtgaacaa cgtaagtcct ggacgtttct tagaaataaa gaaagctgct
     2401 accgtcaata acaagataaa caaaggagct ggaatcacta acagtaaatt tgtaatcccc
     2461 gacatcactg cctgtccaat cgtttgtaaa aatgaaaata ggccagaaaa agttgtcgta
     2521 aaccattctg tgatattttc tacccagttt gccactggta attgatagtt caatagatta
     2581 ttcatgttca ctcacctcgc ttgtttctgc taatgcttca atcacactac ctcggatgac
     2641 gacacctttt aattttccat cctctacaac tgcaagtgga gtcggtgagt cataaataat
     2701 attgaaaata tcacttacca ccatgtcttt cccaatcacc gtcacatttt catctaggaa
     2761 gtcgatcaat ggacgttttt gcttcctagc ttctaacgct tgatctgcag tgatgatccc
     2821 tttcaggtgt ctctttctgt caacagccat cagcatgctg acttcttcat tacgcatcct
     2881 tgtcaatgca acgtttggtc catcagattc gatattcgtc gtcaatgcag gtaccatgat
     2941 gttttgagcc gtaagcactt tggaacgatc gacttcttcc acaaattcac gtacataatc
     3001 atttgctgga ttggtcaata tttcttcacc tgttccgatc tgcatgattt cgccgtcttt
     3061 catcaaggcg attctgtccc caatccgtaa agcttcattc aaatcatggg taatgaaaat
     3121 aatcgttttt tgaacattgg cttgcaggtc cagtaattcg tcttgcattt ctcgccggat
     3181 cagcggatca agagccgaga acgcttcgtc catcagcaag atctcaggat catttgctaa
     3241 agctctggct aggccaaccc gttgttgcat cccaccagat aactgactag gatattggtc
     3301 tttaaatgaa agcaaactag aattttctaa tgctttttct gctttttctt gtcgttcttc
     3361 tttaggtaca ccacggactt ctaacccgta ttctgtattt tccaagatcg ttcgatgtgg
     3421 aaacagaccg aagttttgga aaaccatatt gattttatga cgacggactt ctcgcaattc
     3481 ttctttgctt aacttcgata catcttctcc atcaatatag atactgcccg aagttggctc
     3541 aatcagacga ttcaatagac ggatcaatgt ggatttccca ctcccggaaa gccccatgat
     3601 cacgaagatt tctccttctt ttacgtcaaa attcacgtca tacacaccaa ccgttgcacc
     3661 ggttttcttt aaaatctccg ttttcggctg gtgatcattc atcatatcta aagcggcttg
     3721 tgtcttttta ccaaatatct ttgttaaatg ttttacttct acttttacca aacaaattct
     3781 ccttttttat gtaaattcat ttttctttct gaaaaaagtg accacactat gttaacatca
     3841 tgtagtcact ttgtcaaaaa tgaaagaaat ttgcttgtcc aaactgaaaa aaacttggct
     3901 aaatctttgt aaataaaagg tttagccaag ttaatatttt tttaatattt cacacaatca
     3961 ttctgcaagc tgatgctccg ggtaaaaaaa gtttgtcata cgctgtagtg tatattcatt
     4021 tccaataaaa tgaacgatgt cccctgccgt aaatttagca taggggccag gtgaaacaag
     4081 cagttcttca ttatgagaaa ttgcgataac tgtggcgcca gtcgcctgcc agatattcaa
     4141 ctcgctaatt gaacggtcca gatgcattgc tttttctgat aaagataatt caaacggaag
     4201 taaaggattc atcttatgta ttcgcttcgt ttgattgacc agctgatcca ataattgaga
     4261 aaaatgattc agctcatctt gttgcttctc cacacttgcc atgatttctg cttttgtatc
     4321 ttggatggat tgtacatctt gatattgcaa taggaaatct tttgcttttt ccttcgaagc
     4381 aacaaatgcc ccacttccat gccgtacttc catgattcca agatctacca gcacattgat
     4441 agccttgcgg gtagtctcag gagaagcatt gtacgtactg gcaagtgttg agcgggcatg
     4501 gatcttctca cccactttat atcggttttc aacgatacgt tcggctaaat caacagcaat
     4561 ctgctggtag cgcgggcgga cgattttttt ctttccagac attttctttc ctctttcttt
     4621 ttttactttc cctcatctta ctatgctcta gcaaaataag caaaaaaggg gagaatagcc
     4681 tcttgcaacg actgatcatc gcttgatctg ttcctttttt actgggcaag ctgtttgaca
     4741 gtcatcacaa ttcttaccgg attttaaacg agtatagaca attgcgcctg cacttccaaa
     4801 gattaacaca cttaataaga atgtcgccat cctattcctc tccttctaat gacaatatag
     4861 tataaaactg gttcttggat tttggctgac ggaacaaaac ataaacgagt cctatcatca
     4921 gtaaaatagc aagatacgtc cctaacgcta cctgtccacc ctccaataag tgtcccaact
     4981 gataaatcac aaaactaact aaataagcta aaccgcattg atagcctatg gcgatccaag
     5041 tccatttctt ggcattcatt tcccgatgga tagccccaat tgccgcaaaa caaggcgcac
     5101 aaagtaaatt gaaggctaac aatgaataag ctgctgcggg agtatatgtg tgttgtagca
     5161 cttgccagat ttctctgcca ttttcggata cttccgcatg accaaacaaa ataccaaatg
     5221 tgccaataat attttcttta gcgaccaatc ctgtaatcgc tgcgactgtt ccttgccagt
     5281 ttccccaacc aagcggctga aaaacaactg ccaggattcg cccgaaaaag gctaagatac
     5341 tttggtcttc agaaacaggt tgagcataaa aattataaga agagctgaac cagatcacga
     5401 tactagaaac aaagataatc gttcctgctt ttttaacaaa agacttgctg cggtcatagg
     5461 tttgtcttag gaccgctgac cattgaggaa aatgatatgc gggcaattcc atgataaaag
     5521 gagctgtatc tccagcaaac aaacgtgttt ttttcaagct gattccagag aaaataattg
     5581 cagtcatacc aagaaaatag gcagaaggag ctacccaaga ttgatgagga aaaaaggccc
     5641 ctgctactaa ggcaataatc ggcaactttg cagaacaagg catgaaggtt gtgaccatga
     5701 tcgtcaaacg acgatcttgc tcgttttcaa tcgtgcgact ggccatgacg cctggcactc
     5761 cacatcctgt agcaatcagc ataggaatga aggattttcc tgaaagaccg aatttcctga
     5821 aaagtcgatc catgacaaaa gcgatcctcg ccatatatcc gcagtcttcc agaaagctca
     5881 agcataaaaa caatataatc aattgcggta gaaatcctaa cactgctccc acgcctgcga
     5941 tgatgccgtt caagatcagc tcctgcatcc atcccgctac ttgccattca gctaacaatc
     6001 tgccaacatg ttctggtaac catgtaccaa acaatgtgtc attgatccaa tctgtcccca
     6061 ttgttcctac tgtttgaata gccagataat agatcagcca catcacaaat gcgaagatcg
     6121 gcaaggctaa ccaacgattc gttgcaatct gatcgatttt gtcactcatc gataattgga
     6181 aagaatcatt tgacacaaca cagagtgtac atagttgggt aataaattca taacgttcat
     6241 tgaccagaat cgtctcacta tcgtcgtcca atagtttctc tgttaactgg atcagttgct
     6301 cgatctcttt ttgctgagaa actgctatct cgtaatcctc tcttaccttc tgatctcgtt
     6361 caaaatattt cattgcatac cagcgtttct tgccgctttg ttctcctagt acatcttcaa
     6421 tttcatgtaa agctgcttct aaacgtggat catatgtaca gggaacagga atagaaggcc
     6481 gcttttttgc agactcctga atcttttcga ttagtttttc gattccttgc tttttcaata
     6541 cgctgattgg taaaactggt acaccaagac cataggaaag cttttctaga ttgatttttt
     6601 ttccttcttt tttgatgaca tccatcatat tcagaccaat gatcatagga atgtctaaat
     6661 ccaataactg cgtagttaaa taaagatttc gttctaaatt ggtagcatca ataatgttga
     6721 tgatcagttc aggctgttcc tgaaccaaat aatctcttgc aatcacttct tctggtgtat
     6781 aaggagacaa agaatagatg ccgggtaggt cctgaatatg aatgttcgga ttttttttca
     6841 acgatccttc ttttttttct actgttacgc ccggccaatt ccctacatat tgattcgaac
     6901 cagtcaagag attgaacaag ctagttttcc cgctgtttgg atttccgact aacgcgatat
     6961 tcattctttt tcctcccttt gtatcaagat tagttcggcc tctgactttc gtaacgtcag
     7021 ttcataccct cgcactgtca gttcgattgg atctcccaga ggggctagtt ttcggataac
     7081 tatttttaca tttcgcgtca atcccatatc cattaaccgt cgcttcactg cgccttgtcc
     7141 gtggattgct acgacgatcc cagtctctcc tgcttttaat tgatcaagag aagtccagct
     7201 ttcagaggaa gcagtctttt tattgacaaa gatttgacct agtatttccc gacttactgc
     7261 cactcgatga ctttttacca gtaaaatggc attgttgcct gataaattga cgacgacgat
     7321 tttacttccg gcaacgatcc cgatattttg caaatggcgt tttgtttttt cttctgtttc
     7381 gattcgttcg acttgataaa cgaaattctg ctttgcctgc gctaaggtct gcatattgat
     7441 tcctctcttg tgaaataaat catgtattaa aatcattcat tatatgttct aattttctta
     7501 cgtgttacgc aaataaatga gaatcattct cacatagaat actctcggta attgagaatg
     7561 tcaattatac ttgtgatatt atgagcgaat ctttagtatt gatagataaa aatgagaaaa
     7621 aaatatttct tcatagtttt ccacaatttt ttctctatta aatttgctat tttcccacac
     7681 gatacttgtt ttcagctctt ttttcaaaaa cacaaaactt agtattccac agtttccaca
     7741 acacaatatg tagtgtttgt tgtttgactt ttttactaag agttttatac tatctattat
     7801 agataataag aatgtgttag aggaggcact caccatgaac gtaaaaatct tttctaaaaa
     7861 taattgtatg caatgtaaga tggtcaaacg gtttttaaca gaaaatcaaa ttgcttttga
     7921 agaagtcaat atcgatgaac agcctgaagc agtagactgg ttgaaagcac aaggattcca
     7981 aagtgttcct gtcattactt ctgatgcaga aacagttatc ggattccgtc ctgatcagtt
     8041 gcgtaaactt gccagctaac atgactaaaa gaatcaaaac gtgatacatg gatttgaagg
     8101 gcttgagaca gcaccagttt ttgtcgcaaa cttccaaatc cttttttaaa gcttaaaaac
     8161 aatgcaatga tttgcaatca agcatcatca aatacgaaat gaaggttggg cgtacgtatg
     8221 agtcttaaaa atttacaaga tgtcagttat ttcaagctga ataatgaaat caatcggcca
     8281 gtcaatggtc aaatcccttt gaataaggat aaagaagctt tagttgcctt ttttgaggaa
     8341 aatgtcaaac caaatacgat gacttttcta tctattatgg aaaaaatcaa ttacttgatc
     8401 gatcaagaat atatagaagc agaattcatt caattgtatt caccatcatt tattgaaaag
     8461 ctatatcaat ttttggacga gcaacatttt tctttcaaat cttttatggc tgcttacaaa
     8521 ttttattctc aatatgcgtt gaaaaccaac gacggttccg cttatctgga aacttatgaa
     8581 gatcgagtag ccttcaatgc gctttacttt gcgaacgggg atgaagaatt agcacttgct
     8641 cttgcagacg aaatgatcca tcaacgatat cagccagcta caccttcttt cttgaatgct
     8701 ggacgtaaac gtcggggcga attggtgtct tgtttcttga ctcaagtcac tgacgatatg
     8761 aactctatcg ggcgctcaat caactctgct ttgcaattgt cccgtattgg tggcggcgta
     8821 gggattactt tatcaaatct tcgtgaagcc ggcgctccta tcaaaggata tgagggtgct
     8881 gcaagtggtg tcgttccagt catgaaatta ttcgaagata gtttcagcta ttctaatcaa
     8941 ttaggtcagc gtcaaggagc tggcgtggtt tatttgaacg ttttccatcc agacattatt
     9001 gctttccttt cagcaaaaaa agaaaatgca gatgaaaaga ttcgcgtaaa aactctttct
     9061 cttggcgtga tcgtacctga taaattttat gaattgacac gtaaaaacga agatatgtat
     9121 ttatttagtc catatagtgt cgaaaaagaa tatggtgtcc cattctctta tgtaaatatt
     9181 acagctgagt atgatcatct agttgcaaat ccaaacattc gcaaacaaaa aatcaaagcg
     9241 cgtgatcttg aaaatgaaat ctcaaaatta caacaagaat ctggctatcc ttacgtaatc
     9301 aatattgata cagcgaaccg cgagaatcca atcgatggca agatcatcat gagtaattta
     9361 tgttcagaga ttttacaagt tcaaacgcct tctgtgatta acggcaaaca ggaatatgaa
     9421 gtattaggaa cggatatctc atgtaactta ggctcgacaa atatcgtgaa tttgatgcaa
     9481 agccctaact ttggtcaatc tgtgcgtacg atgactcgtg cattgacatt tattacagat
     9541 gcatctgata tcgatgtcgt accaccgatc caaaacggaa ataagctgaa tcatacgatt
     9601 ggattaggtg ccatgggctt acacacttat cttgcaaaag aacaaatgga atatggctca
     9661 gaggattcat tagactttac aaatatttac ttcatgctgt tgaattattg gacacttgtt
     9721 gaaagcaaca atatcgctcg tgaaagaaaa caagttttcc ataattttga aaaatcagct
     9781 tatgctgacg gcacttattt cgaaaaatac gtaactggag aattccagcc aaaatcagaa
     9841 aaagtcaaag cattgtttga aggcattttt atcccaactg ctgaagattg gaatgctttg
     9901 aaacaagcag taatgaaaga tggactgtat catcaaaatc gcttggcagt agcaccaaat
     9961 ggctcaattt catatatcaa cgatacgagt gcaagtattc atcctatcac tcgcatgatc
    10021 gaagaacgcc aagagaagaa aatcggtaaa atttattacc cagcgcctta tctatccaat
    10081 gagacgattc cgtattacac ttctgcttat gatatggaca tgcgtaaagt aatcgatgtt
    10141 tatgcaatgg cacaacaaca cgtcgatcaa ggcatgagtt tgactttatt catgcgttct
    10201 gaaatcccag aaggcattta tgaatggaag acaacaacga agcaaacgac acgtgatttg
    10261 aatattttaa gacattacgc tttccacaaa ggaatcaaat ccatttatta cgtacgtacc
    10321 tttactgatg atgcagaaga aatcggaagc aatcaatgtg aaagctgcgt gatctaatat
    10381 catagataga ttcgcttatt catattttaa ttaaaggagc attctcatgt cagaaacata
    10441 ttatgaagca ataaactgga atgaaattga agatatcatt gataaatcca cttgggagaa
    10501 gctgacagag caattttggc tagatacacg tatcccactt tcaaatgacc tagatgattg
    10561 gcgtacattg tctcaattgg aaaaagatac agtaggacat gtatttggcg gtttgacact
    10621 gctagatact gtccaatctg aaagtggcat ggatcagttg cgtaaagatg tccgcactcc
    10681 ccatgaagaa gccgttttga acaatattca attcatggaa tccgttcatg ccaaaagtta
    10741 ttcttctatt tttagtacac tgaatacgaa aaaagaaatc gaagaaattt ttgactggac
    10801 aaatacgaat ccttatctgc aaaaaaaagc tgagcgtatc aatgaaatct acaaacacgg
    10861 tacaccgcta gaaaagaaaa ttgccagcgt ctttttagaa acattcttgt tctattcagg
    10921 tttttatact cctctttatt atttaggaaa caacaaattg gctaacgtag cagagatcat
    10981 caaactaatc atccgagatg aatctgtaca tggaacctac atcggttaca aattccaatt
    11041 aggattcaac gagttacctg aagcagaaca agaaacgtta aaagactgga tgtacaatct
    11101 tctttatgag ctttacgaaa acgaagaacg ttatacagaa gaattatacg atccaatcgg
    11161 ctggacagaa gaagtcaaga cattcctacg ctataatgcc aacaaagcat tgatgaacct
    11221 agggatggat ccactctttc cagatactgc caatgatgtc aacccaatcg taatgaacgg
    11281 gatttcaaca ggcacaagca accatgactt cttctcacaa gtcggaaacg gctatttgtt
    11341 aggtcatgta gaagcgatga aagacgacga ttatttgatt ggattggaat aaggatttat
    11401 tttaattcaa aaatttttcg aaaaataaaa agagtttagc gtacagatat tttaaatgta
    11461 cactgaactc ttttttatgc ttattcactt gtttcttcca gcacttttgg actgtctaac
    11521 ttttccgaaa catgttccat tcttctcaat aaatagattt tccggatatt taatattatc
    11581 gtcttaatca ctgcatagac aggtatgcaa atcagcatgc ctaatattcc tgcgacactt
    11641 cctgctccaa tcaataccaa gatgatcgtc aaaggatgga tattcattga tttcccaaat
    11701 aacaacggct tgatcaaatt tccatctaat tgttggatca ctaaaatcga caacgccata
    11761 tacaaagctt gaaatggaga gataaacaaa ccgacaatga ttgctggtac cgctccaata
    11821 aaagggccga catacgggat aatattcgtg attccgcaaa tgaccgcaag caacaagcta
    11881 taaggctgat cgaatattct cattgccaaa aagctcatta caccaataat caaagcatct
    11941 aagatcgtac tgctgatata ggcagataat gtttcgttta attcttttac cgtttggcgt
    12001 aactctttac gaatcgcttt cggaaagaag tgagacaacg catcaataaa tttgtgccca
    12061 tccttgaaca taaatagcaa aataaatgga acggtaaata acaaaataaa aaattgcatc
    12121 ataaatgaaa tgatttttga gacacttgca gataaactga ctaatatgat attgaacaaa
    12181 ttactaagag ataaatttgc tgatgctaat tgttcttcca aattgaaatt tttaaactct
    12241 tcccgctgcg ccaattcatt taaccatttg cttgtttcat ctgcatacat tggcaagctc
    12301 tgggtcaatt gaattgtctg ttccattaac tgaggcacta cattcatgac agttaaaaca
    12361 actaaaatga ttacgaccaa aaacgtcaat aaaaaaccga acactctcgg gatccgatgc
    12421 ttttcgagaa aaacaacgat tggatcgaac atataataga taaaaccagc aacaagcaat
    12481 ggtaaaaaaa ctgccgaaag cattttcaat attggactaa aaatattcgg catttgtaaa
    12541 ataaagtaca cgccgatgat cgtgagtact agctccactg tccagaaaaa taattttgat
    12601 tgtttaaaac gtgattgcat acatctcctc caaggtcctt tgatacttct attctatcat
    12661 ttaacaaaga gttacgcgct gagattttca tttgcttaaa aaaaacaaaa aaaataaaca
    12721 tattttaatg agtagacgag tacttaaaca aaaacacggt ttggagtagt gacaaaaatt
    12781 atactgcagt ccctttttcc aaataaacgg tgttcaaaag cttgcatctt cgtaataagc
    12841 ccagccaaac gaaaaatata aggaactatt tttatttggc tgggcttatt acttgatgta
    12901 ggacatctat tttacaacct cagatcattt attgcgcaat actgactaaa tgcaactttg
    12961 tgatattttt gtgtacggac aactcctcga tcacatcggc ataagtgagc ccttttggaa
    13021 gatcaatcgt atagatattt ttgtacactc ggtagtcatc aacgaatttt acgtcaaaat
    13081 tcacatcttc gattacgata tttttctctt caaaataatc ggtaagaaaa tctttggttt
    13141 cttttctatg gataaattgg atttctaact ttttagtcgt aggtacatgg atcacgcgtt
    13201 tcactactgt gagtgcaata gcaatcccga taaaactgat gatcgcaatg gaatagtaac
    13261 ccatgccgat ggcgatccct aatccagcaa ttgcccataa agaagcggca gtcgtaagtc
    13321 ccgtcaccga ttgtttcgtg acgatgatcg ttcccgcgcc taagaaaccg acaccactca
    13381 ccacttgagc aatcagcctt gcttcatctg aacgaatcgt tccagcaagc tgtggatact
    13441 ctaaagcatc tcttaatgca ccactagcga tctcaacttg gatcaatgca atgatacatg
    13501 ctcccataca aactaaaata tgcgtacgca tacctgctgg acgattttta tattgtcgtt
    13561 caaaaccgat tgctccgcca aatagcatag cgatccctaa acgtaaaata atctctggaa
    13621 ttgtcaatac catatcattc atctaaacac cctccagtcc aatatatcat gtctgaaagg
    13681 agcagacaaa aaaagtgcaa tttttacctc ttacgaaaaa aggactgtga caaaattttt
    13741 gtcatagtcc tttttatgaa taaacggtgt tcaatcagct gaccctcggt attactcaac
    13801 aaacggcaaa atatggggag ctatttttcc tttgtcttct ctaatactca ggtcaaaacg
    13861 ctgatttcac aacctcttta gcctactatt gaaaattaca acttacttgc tgcttcttca
    13921 tcgatgatca caacaacatt gtcgtgattt tgcaatacac ttgctggcaa atgattcgtt
    13981 acaggtcctt caatcatccc tttgattgca tcagctttgc tttcgccaaa tgccatcaag
    14041 acgatttctt tacttttcat aattgaaccg attcccattg aaacagcttt tgttggtaca
    14101 tcttctgctt tatcgaagaa tcgtttgttt gcattgattg ttgattctgt taagtcaact
    14161 actgaagtcg ttccgtcaaa tgaagcacca ggttcattga aaccgatatg tccattttgc
    14221 ccgattccta agatttggat atcgattgga tgtgcatcga tgatttgatc atactttttg
    14281 catgctgctt ccagatcttc tgcttttcca tctggtacaa acgtttcttt gaatggtttt
    14341 ttatcaaata attggacatc catgaaatga cggtagcttt gttcatctga accgcctagt
    14401 ccaacgtact catctaagtt tacagaagtc atttcactga aatccaaatc gcttgaagtc
    14461 atttctttat ataaagtaac tggtgtgctt cctgttgcca atcctaatac ttttgcacct
    14521 ttgttcatac cttctttgat gatttcaaat gctttttttc cgccttcttc agcgtttttt
    14581 acacgaatga tttccatagt gattgctcct ttatattttg gtatagccca attatagcat
    14641 tctttttttc tttttacaat aattagtata gaccaaattg catttttttc attttcttga
    14701 caataaatag gaatccgtgt taggtttcat aagatggttc aatcaaacaa tccatgttgc
    14761 atgaaaacca agcaagtgat tcactgtgga cgctttatgt ctgcagtttt tttatgaaaa
    14821 aggagttatt atgagtatac tgacattaaa caacattacc cagcaatttg gcgacaaaat
    14881 cctctatgaa ggtgtcgatc tgcaattaaa tgctggcgaa catcttgggc tgattggaca
    14941 aaacggagcc ggtaaaagta cattgattaa aatcatcact ggtgagatct taccagatga
    15001 cggacagatc cattggcaaa aaaatattca caaaggttac ctcgatcaat atgtggaagt
    15061 cgatgaaaca ttaacaatcg aagaatttct aaaaactgcg tacgcaaaag aatacgaaaa
    15121 ggaagaaaaa attgcttttc tttaccaaac atatagcgag accttcgatg attccttgct
    15181 ggaaaaagct gggaaactcc aaacggaatt agaccaagga gtgttttatc aaatcgacac
    15241 cttagttgct gagatgagca gcgggttggg gatcgatgtc ttaggtttag attcactgtt
    15301 gaaaaatcta agtggtggac aacgttcaaa ggtgatttta gcaaaactac ttttagaaaa
    15361 cccagatgtt ctgatcttag atgaaccaac caatcactta gatgatcagc atatccaatg
    15421 gctgacgcag tttcttcagg attttgacgg tgcgtatatc gtgatttccc atgaccaaga
    15481 atttcttgat cagatcacca cccatatcgc agacatcgag tttggaaaga ttactaagta
    15541 tactggccat ctaaaacaag cattgaaaca aaaggaacaa aatcgcgaaa gctatttgcg
    15601 ccaatatcat gctcaacaaa aacaaatcga aaagaccgaa gcttatattc gaaaatacaa
    15661 agccggttcc cgatcaacga tggcaaaaag ccgacaaaaa caattagata aaatcgaacg
    15721 gctgacgcct ccagcttcag caagtaaagc tgtctttgat tttccattct cgcctattgt
    15781 cacgacatta gcagtggaaa caactgattt agtcatcgga tatgatcgac cgctacttgc
    15841 acccatcaat ttgaccatcc gctttggtga aaatattgcc attcgaggct tcaacgggat
    15901 tggaaaatct actttattaa aaacactgat cggtgagatt gaaaaaataa gtggcgattt
    15961 ccacttccca gataacacaa aaatcaatta cttttcacaa gatctgtact gggaaaatcc
    16021 attagaaaca ccactacaat atttaagcag tcaatttcca aaagcgacta tcaaagaatt
    16081 acgtcgccaa ctagctaaag cagggttagt taatcaatta gcaagtgaac ctctcactac
    16141 attaagtggt ggggaacaaa ctaaggtgaa acttgcacag ttgacaatga accaaggtaa
    16201 tttactgatt ctcgatgaac cgacgaacca tatcgatcaa gaaacgaaag aaagcttaca
    16261 agacgggata cagaaatttc caggaacagt aatcatcgtc tctcatgaac aagaatttta
    16321 tcaagatctt gtgcaacgag tcattgagat tgaaccaaaa taaacgtttg attgaatagt
    16381 tgaaaaaaag gagctgtgac aaaagttttg tcacaactcc ttcttctgaa taaatggtgg
    16441 ccaattagct gaccttcggt attagctcaa aaaacggcaa aatatgagga gctattttta
    16501 tttggctatt cttattactt gatgcagaac agcttttcac aacctctttt cgtctatttt
    16561 ttattttcga gatttctctt tcctttaaaa acaactgttt gaaagaagaa cgcacatcct
    16621 tgaaaaacaa gcccgctaac cgttacacct aaccagccat actgctgcca cattgtcgtc
    16681 cctagcaatg aaccagccgc acctccaata aagtacataa acataaatac agtattattt
    16741 cggttgcttg ctgcttcacc tagcccctgt actcgtgttt gattggcgac ttgaccaaat
    16801 tgattcccga cgtctaataa aataatagct aaaagcaaaa tggccaaatg gcttccgcca
    16861 accaataaag caataaagct tattgtttgc ataacaagtc ccatatacac gatttttctt
    16921 tcagaataag aatctgccag tcgtcccacg ataggtgcag caactgctcc agacaaacca
    16981 aaaatcgcta atatccctgc ttcgaatgtt ccccaattgt aggcgggact gctgatataa
    17041 aaaatcaacg tagaccaaaa gatcgaaaat gttccaaaca tgaaaaatcc actgactgcc
    17101 gcttctctta atagacgttg agaagcaact aaatgcggca aacttttaag tgaatcaagg
    17161 tatgaaacag ctgtactact ttgctggaca gaacgcggca ttttccagta aagcagaagt
    17221 gtaagaattc cgactgcaca taaagcaaat aaatagatca tccgccaaga aaaaagactg
    17281 gcaacaatac cagataccgt ccgagaaagc aaaatcccca cgagtaaacc gctaagcaat
    17341 gtacccatga ccttccctct tgcttttggt cccgctagta ccgcaccgta aggaatgatg
    17401 atttgcggca cgatcgaaag taatcctatt aaaaaagaag cgcatgcaaa taacgtaaac
    17461 gaaggcgcaa aaaaagcggc caacagagag acggcagata acgctgccat tcgaatgatc
    17521 aattttggac gatccaccac gtcccctaaa ggaactaaaa acagtaaccc taacgcatag
    17581 cctaattggg tcaacatcgt caatatcccg actgcacttg tgctaacaga taaactagct
    17641 gctacttttg ttccaatcgg ctggatgtaa tacatattgg caacaacaac gccacaagtg
    17701 attgataata gtaaaataag cgaagttgtt aaaggttttt tttcattact cttcattgat
    17761 ttcccccatt tcctatctta gtttacaact tatggaaaag attggaaata gaaaaataca
    17821 actattcgcc acacttatta tttgtgcttt tgttttcctt gttttttctt gcgatcttta
    17881 taaatgaaga caactaaccc tactagcaca gtaacacttg caaccaacgt gactgcagtt
    17941 ttgttatatc cgtgaaacag agcttctcca ccacacgcat acaacacaga cgaagggatg
    18001 actccaatca cgatcgacaa tatgatttgc tgtagaggca atttcatcgc atctgcagca
    18061 aaattgacca tagaagatgg aataaccggt atcatatagc ctaacattac acctattttt
    18121 ggatgtttca tttgtcggat tgcttgcacc caatgattcg tttcggtttt cttatctaaa
    18181 aattttaagc gatgcataag gaaaattgca gtgatgttac caagagcatt acccaaaatg
    18241 ttgatgatcg cacctgtcag tggtccaaag ctcaaaccag caatgactcc aactactgaa
    18301 gtaggaatac ctggaacagc acacatcacc gctgtcaata cgatcaagag tatcgatgca
    18361 aaagtcccat gcgaacgaat atgtgcgagt agctctgcct tactcgctct cggatttaaa
    18421 aataattgga tgtctgtctg atattctaaa tacaaccgat agcccactac cagtatcaga
    18481 atgattccca acgagatcaa tatcaatcgg attattttct ttttcatatt tcctcctgaa
    18541 aaaaatctat attcattaaa taatagttta acataaaaac aaaaaagcac cgaaatccaa
    18601 cataggatct cagtgctttt aaatagtaaa aattatttaa cagttacaga tgcgccaact
    18661 tcttcaagtt tagctttcaa ttcttcagct tcttctttag atacgccttc tttaactggt
    18721 gcaggagcgc catcaactac agctttagct tctttcaagc ctaaaccagt tgcttcacga
    18781 actgctttga taactttaac tttttggtct ccagctgcag ttaattctac agtgaattct
    18841 gtttgttctt cagcagcagc agcgccacca gcagcagcta cagctacagg agcagcagca
    18901 gatacgccaa attcttcttc aatagcttta actaggtcgt taagttctag gatagttgct
    18961 tcttttaact cagcaacgat gttttcaatg ttcaatgcca ttataatttc ctccgtttaa
    19021 ttgttctttt ttatttaatg ctttatgcgg caattaagct gcatctccat cttctttgtc
    19081 tgccactgct ttgacagcgt aagccacgtt gcggactggc gcttgtagta cagatagcaa
    19141 catagataga agtccttcgc gatttggcaa ttttgccaat gctgtgattt cttccaatga
    19201 agagacttta ccttcgatga taccgccttt gatttctaat gcttttgctt ctttagcaaa
    19261 ttcgtccatg attttcgctg gtgcgactac atcttcgtta ctgaatgcaa cagctgtagg
    19321 tccagtgaat acttcgtcca tgccttcaag gccagctttt ttagctgcac gtgaaagaat
    19381 agaatttttg ataactttca tttctacgtt agcatcacga agttgtttac gtaaacgtgt
    19441 tacttcatca actgttaatc cgcggtagtc aacgactact actgatgcag ctgctgtaaa
    19501 tttttcgctt acttcatcaa cgattgctgc tttttttgcg ataattgctt cactcatttt
    19561 tgagtttcac ctcctgaatt ttgatggaag agttttttgc actgaaaagg caagtcactt
    19621 ttatagttac tttgctcaac cagtattatt catcacctat tcacttcgtt catagtgatt
    19681 ctactcgctt tgctcgaaag gaaactcctt tcgctttgct caaccagtat tgttcatcat
    19741 ctcttcactt cgttcatagt gattcacaat aaaaaactct atgccaccgc agacatagag
    19801 ggactattga atatattcaa taaacgtcct cggtaggaaa ttaaggcttg atgccaccta
    19861 ctgtcttcgg tacaagttaa ttattaacct caactagctt agcatgctaa actagttgag
    19921 tcaagaatta tttttcagtt ggatcaatca acattctaat atttaattag aaagaagctt
    19981 ggtcaacatg gataccaggt ccaaatgttg ttgtgatcgt tacatttttc atgtattgac
    20041 ctttagctgc tgatggttta gcttttagca atacatcatg gattgctgca aagttttcaa
    20101 ctaatttttc atcgtcaaat gaaacttttc cgattggtac atggatattt ccagctttgt
    20161 ccacacggta agttacttta ccagctttca cttcgtttac tgcttttgtt acgtccattg
    20221 taactgtacc agttttaggg tttggcatta agccttttgg tcctaatact cgtcctagac
    20281 gacctacagt agccatcata tcaggtgttg caacaactac atcaaagtca aaccatccgt
    20341 tttggatttt ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt
    20401 cttttgcttt gtctccttta gcaaaaacta aaacagtttg tgttttacca gttccgtttg
    20461 gtaatacaac agcaccacgg atttgttggt ctgctttttt agggtctacg ttcaaacggt
    20521 atgcaacttc aactgttgca tcaaattttg caatgtttgt ttcttttgct aaagctactg
    20581 cttcggcaac aggataaact ttacttgaat caactttttt taatgcatct tgcatttttt
    20641 tgctcttttt agccattttg gttcctcctt gattgtggtt ataacggttg tacctcccac
    20701 gtgttctata tctttcaata tagagccaac tgagaagctg ctgtcttagg gtgtatagat
    20761 tattctacag tgatccccat gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca
    20821 acgttagctg cgtttaggtc ttccattttt aattcagcga tttctcttac ttgatcgcta
    20881 gaaactttcg caactttatt tttgtttggt tcgcctgaac ctttttcgat tttagctgct
    20941 tttttcaata gaacagcagc tggcggtgtt tttgtaacga atgtgaatga acggtcttcg
    21001 tatacagaaa taacaaccgg aatgatcaaa cctgcttgat cagctgtacg agcgttgaat
    21061 tcttttgtga atcccatgat attgataccc gcttgaccta gtgcaggacc tactggtgga
    21121 gctggagttg ctttacctgc aggaatttgc aatttaacga ttttttctac tttctttgcc
    21181 acgagacata cctccttaag tccgtgatgt ggttaattgg agatgcatat ttctcctccc
    21241 acgttttatc tgatctgaaa aaatccagac aaataatgtg caaaacaatg cacactggta
    21301 cagtatagca aatttatttg ttttttcaag atttttttct gctattttta cattttgctt
    21361 gaataaacgc taaatttatt ctaaaaaaac aacaattttt cgttctcatt tcttctttta
    21421 tgttacactt gcgctagcat ggttcaacag atgaaagcaa acaataacac ggttttttag
    21481 acccttgtac aaagattcct acataacaaa atgaggtgga gaaaatgaaa gatttaaggt
    21541 atagaagtgt ctttgatatt attggacctg tgatgattgg tccaagtagc tctcacactg
    21601 ccggagctgc tagaatcggg aaaatcatac gtagtatatt tggcgaacag cctgattctg
    21661 tagatattta tctatatgaa tcatttgcaa aaacctatcg ggggcacgga acagatattg
    21721 cattagttgg cggcctgtta gggatggaac ccgatgatga acgtttagct gattctttga
    21781 aattggcata tgaagccggt atggaagtct tgtttgttcc taagagtgaa aaagccgaac
    21841 atccaaactc tgtaaagatg cttctaaaaa aaggaacacg aaagctttca gtcactggca
    21901 tttcgatcgg cggtggcaat atccagattt ctgaattgaa tggattcaaa atttctttaa
    21961 gcatgggtac tcctaccttg atcgttgtcc accaagatgt tccaggtatg attgctaaag
    22021 tgactagtat actttctgag accaatacga atatcggcac tatgacagtg acacgggaat
    22081 caaaaggtga aaatgccatt atgataattg aagtagatga tcctcaagta gaagaaacgg
    22141 taaaaagact caaacagctg ccaaatatcg acagcgtaaa ttatttcgat taaggagcta
    22201 aaaaaatgtt tttatcgatt gaagaattag tagatcaagc aaaagcttat ccaagtgtag
    22261 cagaattgat gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg
    22321 agatcatgga aaaaaacctc tcagtaatgg aacaatcaat caacgaggga aaagaaggtg
    22381 ttacctccgt cacaggaatt accggtggtg atgcaaaaaa attagatgat tatttaaatg
    22441 aggcagactt tttaagtgga gaaacaatct taaaagctgt acgcaatgct gtagctgtta
    22501 atgaagtcaa tgcaaaaatg gggttgattt gtgctacacc gactgccgga agcgcgggtg
    22561 ttgttcctgg tgtgctgatg gctgtccgag atcggcttga tttgacacat gaacagcaat
    22621 tggaattctt atttaccgct ggcgctttcg ggttagtcat cgcaaacaac gcttctatca
    22681 gtggtgcaga agggggatgt caagcagaag ttggttctgc aagtgcaatg gcagcagcag
    22741 ctttggtttg tgcaaaaaat gggactcctc atcaagcagc acaagcagta gccattacat
    22801 tgaaaaatat gatgggactg atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg
    22861 tcaaacgtaa tgctttaggc tcttctcaag cttttatctc tgctgatatg gcattagcgg
    22921 gtatcgaaag tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc
    22981 aaatgccgtc gattttcaaa gaaacagcag aaggtggctt agctgttacg ccaactgcta
    23041 aacgacttgc ggaagagatt ttaaataaga gggcgtgata aagctcctta atttcgagta
    23101 ttaagagatt agttatcttc ataacaaaaa acgaagagag gactaattgc ccttctcttc
    23161 gttttttatt aatccatcaa tttcgtatat tttacttcat cgatcgtttt tgttcctgca
    23221 agttgcatcg tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt tactccttct
    23281 gccccaccta gattcaaccc ataaatcact ggtcggccaa tcgcaactaa gtcagcacca
    23341 cttgccagag ctttgaagac atgctctcct cgacggacac cagagtcgaa aatcactggt
    23401 acacgtttgt tgactacttc ggcaattttt ggcaatactt caaaggacgc aggacctcca
    23461 tctagttgac gtccaccatg attggatacc cagataccat ctgcccctgc agagatagcg
    23521 attactgcat cttctggcga ttgtattcct tttacgataa ctggaagatg ggtgatttct
    23581 ttgattgttt tgatgtcatc tggtgtcaag ccttgttttg cagctgcata aatttctgcg
    23641 attccttttc cttcgccatt accacttgca ctttgttcgc tatacgcagc taagttaggc
    23701 attggtaatg gaaattggaa ttgattgatg acgtcttctt cacggtaacc accgagcgtt
    23761 gaatctgccg ttaaaatgat agctttagcc cctgcttcta ctgctttgtt caaaatgaac
    23821 tcattgaatc tgtcgtcttt actcatatat aattggaaaa actgcggtgc gcctggcgct
    23881 gcatcggctg cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa gattgatcca
    23941 gcagcagcta ctcctttagc tgtatcagct tccccttttt catgtgccaa tccttgggcc
    24001 gctgatggcg cttggataat cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa
    24061 tcggctgagt cgattccgcg taatatccgt ggcatgattt tctttgtatt aaaagaagtc
    24121 gtattttctt tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct
    24181 gcttccatgc gctcttttac acgaccttct agttaggcaa tgtttacaat atcgattgga
    24241 tgttcttcat tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca
    24301 tataaatcat actaaactta caaaaagtaa gcaaacattt actctcagat acttttatcg
    24361 ctattctttc caaataaatc gacttacaag ctgatctact tcttggtttt catgaagcat
    24421 actatgagag gcattttcac ctgtcactac tttttcctct atttcatttc cgcgttgctt
    24481 caacaacgca tagaccgcta atgcactgtt taacggtact gttccatcac tcaagtctgt
    24541 ttcatctagt tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat taccgatcat
    24601 ttgctggtaa tcttgatagc ggttagaaac gacagcaggc ccctttgcta actcatctgt
    24661 cagagactgc gcactatcat cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt
    24721 ctgaacttgt ggcaatgacg tatcctgtcc atacgttcct aaatagcgta gtgcactaac
    24781 tcctcccatg gaatgcccca ctatgttgac tttatctatg ccgtagtttt ctttcagata
    24841 caaaagacaa gttttgatcc attctgcttg attccactcg ttatttttat tatcttcaaa
    24901 caatacttgg accatcggat tgtctgcttt tccagacaaa tctcctgttg cttgtatttg
    24961 tccatctgtt cctactgtta aaaccaattc ttttactgct tgctgatttt tttccaaacg
    25021 ccgcaacatc cctttaaagg aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg
    25081 aatagtggtt ttttctatca ctttactgga ttctgccgtt ttttctttat cagaaacttc
    25141 agtagtattt atcttcgtcg tctggcaacc acttaacgca aacaaactga taaggactaa
    25201 agctgctgca gttgcacgtt tgcataaatt tttgattggt tctgtcatac acctcttcct
    25261 ttccatatag aaaaaagaaa gacttgaaag gccaatcata tgatcgacat ttcaagtctc
    25321 tttattaatc gattttatct acttgatcga aatcaagttc tgtactggtt tcacgtccaa
    25381 acatatcgat gttgactttc aatttttctt tttctccgtc aacttctgtt acttgtcctt
    25441 ctaatccagc aaatgcgcct tcgatgattt tcacgacttc gccaacttct acttctagat
    25501 cattttgtcg caggctcata ccgatagaac gcaagatatg attgacttct tctggaagta
    25561 atggtgctgg tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt
    25621 tgcggacaac ataccatgaa gcgtctgtca tgatcatttc aacaagtacg tatcctggga
    25681 atgttttatg aacgatttct ttttctttcc catttttgac ttcagtttct ttttcttcag
    25741 gaacaacgac acggaaaata aagtcggcca ttttcatact ttgcgcacga gattcgatat
    25801 ttgcttttac tttgttttca tatcctgaat atgtatgcaa gacataccag tttttttcaa
    25861 aagattccat tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag tacacccgaa
    25921 gtttttcttt cgcttcatta tagcactaaa agtacgttgt ggctagcgag ttattttaaa
    25981 atccagccaa ataagctttg gatggctgta tccattacgt aaaacatcaa tccgaaaata
    26041 atagaagttt cgatgactac taacgtatct ttacggagtt gttttctgct tggccaagtt
    26101 acttgtttga tttcatcttt tacgcttttt agaaatttca tgagacaacc tcccttgcta
    26161 ctgtttattt cgtttcttta tgtaatgtat attgattaca atatttacaa aacttattga
    26221 tttccaaacg ttcgccgctt tttccttcac tgactgattt tgtgtagttg cgagacccac
    26281 aaacggaaca agcgagtgct gcttttttct ttgccattta cttctgcccc cttgctgaga
    26341 atcagactta tacttcataa cagtatcacg taacttttta cttgtcaatt tgtgaaataa
    26401 actatctatt gttttggata aaattatgat atgattaatt tgaaattaag ctaaaatgag
    26461 acaattggag gtatagcgat gctattaaaa acacttgtat ataagaaaaa agatctgaca
    26521 accgttacag agactagtac attagaagaa gcattaaaaa ttttagaaac ttccggatac
    26581 cgttgtgtgc cgatcttaga ttcttcaggt aatattttcc gtggaaacat ttacaaaatg
    26641 catatctatc gtcataaagc aaatggcggt gatatgacgc ttcctgtcac tcatcttttg
    26701 aaaaacgcta caaagttcat ttacctggat acttctttct tcaaagtatt ctttacgatc
    26761 aaagagcttc cttatattgc tgtccttgac agtgaaaatc atttttatgg tattttgaca
    26821 catagtacgt tgcttaatat gctctctcaa tcttggagcg tagaacaagg aagctatgta
    26881 ctgacaattg cttccacagg aaagcaagga gatttggctg ctattgcaaa aatcatttca
    26941 aaacacagca gcattgccag ctgtattact cttgatatcg aaaaagacga atacattcga
    27001 cgtacattga tcacgctacc ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac
    27061 cacttagaga aaaagaatta taaagtggta gaaattgaaa atttgcaaaa ccaagaagca
    27121 taatcaaaaa gcgaaaaaag aaaacagcta atctgctttc ttttttcgct ttttataatt
    27181 agacacgctt ttcaaaatca agcgcgcgat ctaaataata catcaatggt gctgaaacag
    27241 ccatgacgat aaactcactt aatgcaagcg tgccataggt tgtccaaaat gcttggccac
    27301 ctgttggaac taacatataa gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca
    27361 atactaaacg catctttaca tttttcactt tgttttgcaa taaagctgtc aggcctaaag
    27421 aaagcaatgt ttggccgcca ccaaaaacca catccatgat accaaaacca aagaaggcat
    27481 tatagacgat gaccccgcct aaaactcccc aaagcaattt acggttaaag acaactaaat
    27541 gattcaaact ttctgaaata cgaaactgga tcggccctga ggatacaggc gcaactaaaa
    27601 tagttaacgc tagataaaga gccatgatga tgccatttac aacgattacg gataaccgtt
    27661 gtctgctttt cgttttttgc attttctctc ctcttttctc ctagtttttt taacgcggga
    27721 tggttgatga accgcggtta tgtgaaacaa acaataatta taacagactt ttcatttatt
    27781 tcaatccgtc ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa
    27841 aaatcgtggg atatggtata aagtaagtgt aaggggtcat aagaattccc agtaaagcag
    27901 acgaagtagg atctgcaaga acagaattgt tgtgaaactt aaataaataa aaaaggatgt
    27961 gggaatttga gcaacctctt actagagaac ggtctagcaa gcagctgaag atctaagtgg
    28021 acatgccgaa ccttggaact tctccttacg taagtaatgg atcatgcgct tgctaggctt
    28081 ttttgtgttt ttcggtcttc ggaccttttt cattttccta gacttctgct ataataacca
    28141 agaattaatt ttgtaacaga tttttttgaa aaaacaagtt tctttcattc aaaaaataga
    28201 aaaagcttgc taattattgg ttaaccagca aacaggagta ttaaaatatc ttgttttatt
    28261 gaatagcagg aggaaatcat gaaattccat tatcatatat tgatcaatcc atcggcaggc
    28321 agcggaaatg gctataaagt agcagaaaga attttaccgg tattaaaaaa taagcatatt
    28381 gactatacac ttcattatag tgaatacaaa ggacacgatg ctgagattgc tgaaactttg
    28441 gcaaaagaaa cattgattcc ttggatagaa gaagaacaga aaacaatcga tactttcccc
    28501 cttttgatca tcgtaggagg tgacggcacc ttacatcagg tacttgatac tttctatcag
    28561 atggaagtag aattcccagt tgcttatatt ccagcaggtt ctggagatga ttttgctcga
    28621 ggggcaggcc ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc agctcaatca
    28681 ccagaaaaag tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct
    28741 gttaataatt tcggtatagg attagatgca gctatcgtcc atgcgacaaa tcattcttcc
    28801 acaaaaaagc gattgaacaa atacaactta ggatcatttt cgtatttatt ctctattctc
    28861 cgtgctttat tcactcaaaa aggatttcca attctggtag atgcaggagg aaaacaatta
    28921 agtttttcaa acgcattttt atgtacagca acgaagcatc ctttttttgg cggaggaatc
    28981 gcgattgttc caactgctga tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga
    29041 atcaatttct ttaaaatctt gtggctcatt cttttactta tacagaaaaa acaattgaaa
    29101 tcgaaatatt tccatcacta cacaacaaac agattacgta tcgtttcaac aacaccgcaa
    29161 catggtcaag aagatggaga agaaatggaa aaacgacctt ttgatatcac aataaccaca
    29221 actaaacagt tgttttggtg ttaataaaag aggttttgtc agaagctaca tctggaacac
    29281 cgtttattcg gaaaaaagga ctgtgacacg acttttgtca cagtcctttt tttactatgc
    29341 agttaattct ttgcccaatt gggttgccat cgatagtttc tttctttacc caaaataaat
    29401 tggatcattg aaaacaccgc agcaaaagca actaagacac aaccattgaa ccaaaaagat
    29461 tctgcaaatg tttgattgta actgagtgtt tcactgatca agttattatt ttgaacaaac
    29521 caatatgttg gtgtgaatgc cgctattttg ctaaccattt ctggcaagta ttctgctggt
    29581 acaaacaccc caccgataaa gcaactgcct gtgataaaaa tatctttgat cccaccgctg
    29641 gtttctgagt tttttaccaa tgaagtgata caagccgaaa agctaatgat cggcaataaa
    29701 aacaagaaag tatttaaaat atgataccat accgttatat cccacgaggt gtggatcact
    29761 aacagaacca ttagaagtaa gaagccactg aacaacagca gcgagtaact aatcaaacta
    29821 aaggttactt tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt tcgtttactg
    29881 atctctttgc gattaaatgc tagattgatg acgctgaaac cgctaaaaat ggatagaaac
    29941 aacccataag acaagaaatt aaaggttgtg cccgtcaaca gctttttcat ccgctgacta
    30001 tatcccgcac ttagctcaac ttctccagta accgagagtg ccatttgtgt ctgttccata
    30061 gctgcatcga ttgttttatc ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc
    30121 acttgctgtg taacaactgt tttagaaaag gcatctgggc gactctgtat cttgatcgtc
    30181 ggcatctttc ctacttcgac ttgttcggta aagtctgcag gcagatgtaa gataatgttt
    30241 acacgattaa aataaagagc atcatcgatt tctttttgtg aggttccatc caatgtaacg
    30301 actgtttcct tgtcattcaa atatttacga aggccatcga ctacaggcga ctcttcttca
    30361 ctaatcaagg cgattttcgc atcttgcaga gaggtatttt caacatcaat attttgcgca
    30421 aaaaagaatg tggcaacaac agtgataatt acacctatca gtaaattcga tttatttgct
    30481 ttaagcaatg tccaaatcgt tttataaagc gtcataacgc acccctcttt ccatccaagc
    30541 atttcctaac gcaaaaagga aaataaatcc gatcaaccat agaagattgg tataaaaact
    30601 agccaaatca gtgtagaaat aaagtttata caaactttcg ctgatcaagt tgacgagatt
    30661 taattgacca acaatcggaa tatgaagatc aatccaatac ttcacttgtt ccgaccccat
    30721 cataccagca aaaaagctca tcgcaatcgt cactgaaatc aagatgctat ccttttgcac
    30781 cagattcatt tttggtatca aattaccaac caatgtacct aacaaaatcg cacacaacga
    30841 ccccagagcg gtaacaatca tcaaccattg ccaacgttca ccaaagtcta cttgatagat
    30901 aaaacgaaag acagctaaca taatataaat ctctgtcaaa acaatcgtaa agcttgctgc
    30961 caaattagcg attgaaacta acgattgctt tctaggaatc aaggacaagc gaataccatt
    31021 tgacgattga tttgcttgct gatcctgtac attgcggacc ccccacatcg tcccgtagag
    31081 aatcgccatt ccaatcagag tgaagaaata aaaacttttg aaactcatat tacgacctgc
    31141 atgaacttct tgaaaagata cttcttgttc aaaaacagtc atgatgggcg cgagatcaac
    31201 gttctccact tctgccactt tactcgaaat tataccaata cgctgtaaaa attgatccat
    31261 gacgtttttc atcaacgtct gactcattcc tgcttgaccc acatggagcg aaacagatga
    31321 accatcaatg agataaaaac cagccaaatc atctgcattt agcttttcat tcgcttctac
    31381 ttccgatagt tcctgtactt caaataatgg tttatcattg acttcgactt gttctaagac
    31441 tgtggtaaaa ttttccgaaa gcgctgtttg atccgctttg attacaattc cagtattaat
    31501 cgtttcgaga ttgccacctt gatcaatctc gccaaaggcc gccataaaca tcaatcctaa
    31561 aagaattgga aaggccaatg tccaaaacaa catggattta tttcgcagca gtatttttgt
    31621 gcgatattta taaaaatgcc aaaacattat ttcccctcct ctagtctctc aaatccttgc
    31681 cagtaatctc taaaaagaca tcattcaagg acgcttcttt ggttgctatt tttccgtaac
    31741 tgatcttttc ttcttgtaag attgtaagaa tcggcaacaa tactgtattt agttcagtag
    31801 ttgttacttg gagctgacct tttttataag cgattccatc agaagctatt gttcgctgac
    31861 aacacgtttt ctgggagtaa tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata
    31921 tcttttagag attctttggt tcctttagca atcacctgtc cttgatccat gatcacgatc
    31981 tggtcgcaaa gaatttctac ttcttccatg taatgtgttg tatagacaat ggttgctcct
    32041 tgtcgattca gctccttgat actttctaaa attttattcc gactttgggg gtctacagct
    32101 actgtcggct catctaaaaa aattaaccgc ggcttgtggg caatcccaca tgcgatgttc
    32161 aaccgacgtt tcaagccacc actaagttct cctggacgaa acgaaacata tttttctaat
    32221 cccactaatt gaatcacctc ttcaatcaag gctttccttt tctctttttc ggaaacatat
    32281 aaaccgcaaa aataatcgat attttcttgc acacttagtt cgtcaaagac cgcaacgtct
    32341 tgaggaacga ccccaatcat tgccttgaca gcataatttt caggagtcat tgttgtcccg
    32401 aacaatttaa tggttccttt gtcataattt aacaaagaaa gcatacagtt gatggcagtc
    32461 gatttcccac tgccgtttgg tcctaacaga cctaagactt ctccttcttg tacttgcaga
    32521 tcaaaatgat tcaatgctac aaaatctcca tagcgtttga ctaatttatc aatttcgata
    32581 atcatcatga aaaactcctt ccaaatttct tgttgatctg actataataa agtgcaagaa
    32641 aagaaactag tgacgattgg ctaaaccaca catgacattt gtcacaatga aggagtctgc
    32701 tatgcataaa tctcggctac tcaacgaaac tagctttcta ctggttatct cattgctttt
    32761 gctggtaagt caaggattcg ttgctatgca agttatctat ttattgatcg ccgttttatt
    32821 cactggtgtc agttatttac tgcctacaaa gccaaattgg attttccaag ttattctatt
    32881 tcttttatgt gcctttttac ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat
    32941 cgcccctgaa gaaagccacc ttgattactt gtttttgtta ggttttttgt tgatttcgac
    33001 ggttggtaat catcccccca tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt
    33061 ttacttacga aaaaaagatc aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa
    33121 agatactcat tgggaaaaac aacaagagtt atcggaacaa aataaagcat tggccctgtc
    33181 acaagaaaca caattagcct tgcaaattgt ggaagaacgg aaccgaattg cccgagacat
    33241 ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta caaattggtg cgcttgaaat
    33301 gatcaatcaa gaaccaaaac taaaagaacc attagaaaat ttaagtgcca ccgttcacac
    33361 tgggatggat cggattcgtc aaagtgtcca tgacctccat gagacatcga tctcttttca
    33421 acactcctta gagtttctga tcgctgattt tccttcttct gttacgattg aaggaaactt
    33481 gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta atggtcatca aagaagcctt
    33541 aaccaacagt atcaaacata gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc
    33601 ggccttttat cgcttgcaaa tcacgaatga tggcaccagc ccacaaaaaa attctgctcc
    33661 cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa caaaagggac aagtccatat
    33721 tttccaagaa aaccagcaat ttcagttaac gatcatttta ccaagggagg gacaccaatg
    33781 ataacagtag tcattattga gatgatccat ttgtgacgaa atcgttacaa accattttag
    33841 aaagcacgaa agagatccgt gtgttaggca ttggtcattg tgccaaggat gcgctagtct
    33901 tatacgaaac ccatcgtccc gatgttttgc tgacggacat ccgcatgcct aaacaaacgg
    33961 gcatcgatgc tgccaaagaa attttggcta aatttcccaa agccatcctt ttgcttttga
    34021 cgacattcaa agatgaagaa tacatccgcg aagcattttc catcggtgtc aaagggtatt
    34081 taatcaaaca gaacttgcaa gcgattattc cttctgtcaa atccgcttat aatggacagg
    34141 ccgtatttgg taatgaaatc atcgagactt tcactcaaat gatgaagaat aaaccgacga
    34201 ttgatagtca tgcttatgct tcttttagtg aacgtgagct tgcaatcatc aaagaagtag
    34261 ctgccgggaa aaacaataaa gaaattgcag atgccttgta cttgagtgac ggcactgttc
    34321 gcaattacat cagccaatta ctagaaaagc tagatttacg ggatcgcacg caattggcta
    34381 tctattttta tcagcatttg caataagtct tatctaacac aaaaaaacct gaatgatgct
    34441 ccttcgcatc attcaggttt ttttcacatc ttctattcaa ttgtcccgca aaccatttcc
    34501 tcacgattga aagaatcaag gattcgcagg gctgccagga ttcgttggat tgttcggcgt
    34561 gttaggcgga aaaataggcg ccttgtctaa cgttttttct agttttgatt cttcacgatc
    34621 gtctcgttct acatcaggac tttcattcaa tttgaattct ttttccatct catctaactt
    34681 tttaaagtca ttgccagatt cttttgctaa tttcgctaat ttatcttcgg tttctttagc
    34741 cataaagtaa cgcccccctt ttgatttcag cttagcatat cacactgaaa aaacaaaaga
    34801 aaacgctaac caacgatcat ttttagctat ttctcatctt taaaatcaag cattttttgc
    34861 cggtataaaa tcgtagggag aactgttttc cataccaatc atcggatcta ctaactggtt
    34921 atcgatcact tctgctgtaa gaacgaaatc tttagaaaca gcaggttcta cctcttcttt
    34981 cttttcttct ccaacaaatg aagaaaacag atctggttcc actgtttcca ttacttggtt
    35041 tttcgtttca ttcgtcccac tgtcatcttc tacaggtagc ttttctttta caacccgtgg
    35101 ctcttttgta tagttcccat cttcaaatgg atcatttccg accaaagaat cttcataagc
    35161 tgctagtttg gtccggagag tgatattcat tttatcatta tccatgatcc ttgatttcaa
    35221 cgtagtcatc cggcttgctg attcattcgc tacacaagtg acataggctt gctcttcccc
    35281 taacaaaatc aacagttcct tactgcgagt cactgccgtg tagagcaaat tacgctgaag
    35341 catccgctga tattgatgga ccatgggcaa aataaccatc ttgaattcac tgccttgtga
    35401 tttatggatt gagcaacaat aagaaagagt gattttattc cattcgtttc ttttataagt
    35461 gacctcattt gcatcaaact ggataactaa ttcatccact ttatcttcag aatcttttgc
    35521 caagatgata cccgtgattt ctcccatatc tccattgaat acattcaatt cgggagtatt
    35581 gactaattgg aggactttgt ctcctatccg ataaacggtg tcgttccaat gtacttcctt
    35641 ttttccaggt ttccctggat tgaatatctc ctgcatcatt ttatttaacg catcgatccc
    35701 tgcaggtccc cgatacattg gtgctaatac ttggatatct tgtgcacgga atcccttatt
    35761 tttcgctttt tccacaattt tagagatcaa gggttcaatt tgataagcat cacttcggaa
    35821 ataggaacga tctttttgat tctttgtgaa atcttttggc agacgtcctt ctttgatctc
    35881 atgggcaaga ggaataatac tagaaccatc accttgccga taaatctgat tgagttccat
    35941 tttgggaatt tgttcgattt ccaaaagatc atggagtact tgaccaggac cgacagaggg
    36001 aagctgatct ttgtctccta ctaagataac ttgcatattc gtcggaatcg ctttgaataa
    36061 tgtgttagcc agccatgtat cgaccataga catctcatca acaatcaaaa gtccgccttc
    36121 taattctttc gtactgactg aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg
    36181 gatcgtactg gcaggcagac ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg
    36241 tgctgcaagt agaataggaa aaatttcttg ggtatatttt gaaggatcca attccagtcc
    36301 gttcaactct gcaaaaagat tgacgattcc gttgatcaca gtagttttcc ccgtacctgg
    36361 tcctcctgtt aaaataaata gaggggaacg gattgcttca gcgatagctt gttcttgcga
    36421 aggtccatac tgtatgccga atattttttc gatacgccga atattcttct gcacttcttt
    36481 ttcaggataa tggatttctt ttttacgact gagaaggcgc tggatcgaat taccgatccc
    36541 ccattcagaa aagtacaaac tgttttcgta aagattagtc tcttcttgtt gtatctttcc
    36601 ttcttcaaca agttggataa ttacagtagc aacctgatca ggctggattt caattgggcg
    36661 acttgtttct aaaatacgga tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc
    36721 tcctgtttga actgcatgct gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg
    36781 tgaatctgct gcaataccga tttgttcggc tagattatct gcacgtttga acccgattcc
    36841 ttctatgtct tcgactagct ggtacggatt ttcttcgatg atatccaaag actcgttctt
    36901 ataggcctgg tagatactga aagctaactg actaccgaac ccgtaacgat tcagtcccat
    36961 gatcacttga tccatcccgt ggtttagccg aatcgtctca gcaagcattt ctctttttgg
    37021 cttcgtcaaa ccggagattt gttttaatac cgaaggatca gcaatgattt tttcgatagc
    37081 atcttctccc agcaattcta caattttttc tgctgttttc ttcccgattc ctgggaattt
    37141 ttcgctggaa agatacgcta cgatcccatt tgcagttgtt ggcttttctt gctcatatct
    37201 ttcgagttta agctgccttc cgtatttagg atgatccact aattctccat aaaaacgata
    37261 agtctcattt tcttgaatat cgccgaaact tccggtgact acaatctctt tttcacggta
    37321 atcagctgtg gtttcactga tgtgtaccaa aagcactttg taaaaattac taggattttg
    37381 aaaaaaaatc gcggcaactg taccaacgaa ataaggcatt tcttctggct gcatcggaag
    37441 ccgcctcctt cttctatagt aaaggttctg gtgctgtttc agttcctaaa aaatctgttt
    37501 ggttccatac tttcagttca tatggcaata atctgcaagg ctcttcagtc tctaaaatcg
    37561 tcaaactact attgaacaat ccacccattt cacgcaactc acttagctct tttcctgtca
    37621 tccattgaat ggctgcagtt aaagaagctc catgtcctac gaataaaagt gggccatctt
    37681 tatgttgggc aaccgcatct gctacaacag tttcgatacg tgtgattgcc gattcaatcg
    37741 tttctccgtg aaaaggagaa ggatcataca gatctaaatg attacgcaaa ttcggcagat
    37801 tatccggata tttctggtac atttcatcga tcgactgacc ttctaaagta cccagcccta
    37861 gttctcttag cttatctgta taaaaaatct ctaccggacg agcaagctct ttgttgatac
    37921 cttctgctgt atcacgtgct cgctttgctg tactggaata aattgcttta aaaggaacat
    37981 attttacctt tttcccaaat gctttgattt cttcgtaact ttgtggcaaa agcggagaat
    38041 cgccattgct cccttgaaaa cgtctttcct gattccattg tgtcttccca tgtcgggtaa
    38101 aatatagttc catacgcagc aaactcctgt ctttctttta ttttactcta taataaactg
    38161 tctaatccag cttgtcattt tcatagtgat gcgtcaattc taaattaggg aatccttgct
    38221 ggcgaagtgc ttcatacact atcattgcag ctgtattcga taaattcaat gagcggacat
    38281 gctcatcatt catagggatg cgtaaacact tttcttcgtt ttcacgcata aatgtttctg
    38341 gcaaaccggt tgtttctttt ccaaataaaa agaagtgctc ttctccatca gcaaaatttt
    38401 cgtcactata tactttatgg gcaaattttg agatcaaatg tagtttacga ttacctaaaa
    38461 atgttaaaaa agcttctaaa ttactgtggt aagtaatatt tacatcattc caataatcca
    38521 accctgcccg ttttagatgt ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa
    38581 gcggagtatt cgtggctgca cacgtacggg caatatttcc agtgtttgca gggattaatg
    38641 gttcaaataa aacgatatga ttcatcagga tcattcctct catatttatg ttcgtttcta
    38701 ttttctgaca atttgatttt tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc
    38761 gagtcgttac gttagtgaag ctcttctcac taacttttat cagaacagga acctgataaa
    38821 aaccaatgaa aaaacaactt gttgctaata taacaccctc aacttttttt tgcaacctct
    38881 actcattaaa aactgctaga aaaacgtttt ctcttagttc cttttaataa ttctatgtca
    38941 attgtaatct tcttcaaagg attcttatat aagtctgctt tgattcgctc ttctacctgc
    39001 cattctaaag gtgacatggc caaaagatct ttttgatttt cctctggtat ctcaaattca
    39061 taggttacac gctgacgttc agttaatttc attgcttcag caaacttggc tactacacgt
    39121 tcattggaat aactctgttt ttcaggttga ttcggataaa aagcctgacg tagttccttt
    39181 aaataatttt cttgtggcac caccttcaac acttctccat cttctgagag tacacgttgg
    39241 aattcttggt aatgagaagg cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca
    39301 aacggcaaat tggtcaaatc agctacacac caaaatgctt cggcagattg ttctgtagct
    39361 gcgtagactc cttcttttga aatatcaaat ccaacagcat tttgaatctt tttcaatttt
    39421 gaaagttcat tcaagaagct accttctccg cagccaacat ctaatagcga gacttcttca
    39481 ggtaaatgct gcgcaatagt ctcaataacg ggctgataca tgccagattg gatcattcta
    39541 cgccttggaa cgaacattcc ttgatcgtat tctgttttaa ccgcatggga taaaaaataa
    39601 agtgttcctt ttttggaaag gtcaaaacgg tgattatttt tgcatatcaa gccattatca
    39661 gactttttta cttcttcttg gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa
    39721 aactggcgtc ctcggtcaat tttttttaac atagttcccc ctcatttctg ttttatctta
    39781 ccgttagatg ctcactttgg caactgctaa aaatatgata caatgcctag aagaaagtga
    39841 aagataaagg agaagattag atgatcaaag aattttgcgc tgaaaactac acatctattc
    39901 ctctagcaat tgctagagga gccgatcgga tcgaattgtg tgacaactta gcggtcggtg
    39961 gcacaacccc gagtactggt gtgatcgaag aaacgttaag ttatgctggg gaaaaagata
    40021 tccccgtaat gacgattatt cgtcctagag ggggcgactt cgtctataac gatatcgaat
    40081 tgaaaatcat gcatactgat ttgattgaag caaagaaatt agggacggat ggcgtagtat
    40141 ttggttgtct tactccttca ggctggctag acgaagaggc cttagaacta ttgatcgaaa
    40201 cagcagaagg gttgcagatc accttccata tggcttttga cagtattcct gaagatcggc
    40261 aattcgaagc aatcgattgg ctggtagaac atggtgttga ccgtatcttg actcatggcg
    40321 gtcctgctga caaaactatt gtagaaaatt tcccacggct taaagaattg attgcctata
    40381 cagatggccg cttaatcatt ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg
    40441 tagctaaaga attgaacgta agcgaagtac acggaaccaa aatcgtggat cttcagttat
    40501 aatcttataa acaaaaaaac atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt
    40561 tttttacata aaatagccca taaataaaga agcaatattg aaataaatca gtacactcgt
    40621 caaatcgctc aaagtcgtga taaagggacc gcttgctact gctggatcaa atcctagacg
    40681 atccattagc atcgggatta aactacctgc aagattcgcc actgtgattg caaatagcat
    40741 agccatccct atgacaaacc ccaagataaa attatgtttc caaaaaccta cgattagaaa
    40801 aatcgtgatc cccgtaacag ctcctgtgac aacgccggtc aagacttcag ccaaaacaag
    40861 tcgaacaaat ccatgatctt tctcatcaga aatcgcaagt cgacgaacag ccacagctag
    40921 cgactgggtt ccagcattac cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat
    40981 actcgcttca cttaccaagt cttcatagtg actgattaaa gaagcggtcg acatacctaa
    41041 aaacaacaga gtgatcagcc aaggcaaacg tcgagacgct gcctttactg gattttcatt
    41101 aatttcttcc acattgaccc cggcaagtcc tgaataatcg ctagcagctt catcatcaat
    41161 aacgtcgatg atatcatcga ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac
    41221 aggaagagct aagaagtcat aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc
    41281 tacatgaact gaaagcactc gttcgctcat cagatcagaa atcattgcgt cgtcatcatt
    41341 cacgattagg tcgcgcagag aaataacccc tactagctga ccttcttgat taacgacata
    41401 gatgtaatag attgtttcag ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg
    41461 aacagtttga ttggctacga tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc
    41521 atcctcgtaa tggagaagtt ccttgatttc actggcatcg tctgccgaca tcaaacttaa
    41581 atatttagca atttgctttt tatccagcgt attaagcaag tcaacagcat tatctgtata
    41641 catctcagaa agcatatcag ctgcatagct aggacgcatt tcatctaggt attctttcat
    41701 atgttcgttg tcttcttcaa tcacatcgaa catatccgct aattctttag gcgacagata
    41761 ggaatagatc agctgcctgt tctcttctgt aagagattgg tagaattgcc cctgctcata
    41821 gatatgcatc tctaaaaaac tgtttctgaa tgcctgaata ttattttccc ttaaatcttg
    41881 agtcaactgg gcaaatcttt cctctaattc ttgattttca tccaaaccaa tctcacctcg
    41941 ccttattctt caaaccgtct cacaactgcc tccatgtctg cggctaaagg ctgcttgaac
    42001 tccagcaact cttctgtaaa tggatggtaa aaacgcagat cataacaatg aagggcttgt
    42061 ctttttattc ccagatccat tcgcccgcca tacatatcat caccgagcag ggcacagcca
    42121 attgcggaaa aatgaactct tatttgatgt gtccttcctg tatgcagctg gatatcaact
    42181 aacgcgattt ctgttagtcg cttctctatc cagtactcag tctgtgccga acgtccttca
    42241 tcagagacca ttcgcttcaa tagcgaagaa taatctcggg cgatcggcaa atcgatatgt
    42301 ccatgtgccg ataaatctga aagccttcca ctgactaagg cctgatattt tttcacaaac
    42361 ttcttttcac gaagttgcat atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc
    42421 atcaaaccag atgtatcccg atctagtcgc gtcaccacat ggattacttg gttttcgtaa
    42481 ccttgttttt tataataagc ctttacacga ttagccattg tcccatttgg atggtattgg
    42541 gcaggaatag acgaaacgcc tgctggttta tttactacca gaacgtgttc atcttcatac
    42601 acgatatcga tcggtgtttc atccaaaagt acggtttcat gttctccttc agcaggaatc
    42661 acaatggtta ccttatcatc cttagctaga gaaaacaaga cattttctac ttgatcatta
    42721 actttgattt gaccgccttg aaattttatt ttcgctaaca gaccctttga gatccctttt
    42781 tcttttaaaa aatattttac ttgctgagaa tgatctttct cataatccca tgtaaactgc
    42841 atatcaatcc tcactaataa aggcatcttt cacacgatgc caaaaatgca tatgtcgata
    42901 agaagcaaaa tggatccgtt cttctgctat tcgataataa attgagcgga tatttgcttt
    42961 tgcgacattc aattgatcaa ccgttacaag ataatcttct gtattttcca attttatttc
    43021 tagccattca tcatgggcaa tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt
    43081 cagtgaagca atctctgcca actgaaaggc attgatacta ggatggatca cagcaccgcc
    43141 gacactttta ttatatgctg tcgaaccagt aggcgtagaa atcgtcaatc catctcctct
    43201 aaaactttca aacagctcgt ttttgatgta tacatcggct accatcgtac gattggcccg
    43261 tttaatcgtt gattcattca gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact
    43321 gatccgaaca tccagcaagg gataactaac actttgttcc cggttcgttc gtaagctgtc
    43381 tactagttct tccagctcgt aatcccgcca atccgtgtaa aaccctaaat gacctgtatg
    43441 cacgcccaag aaacgcacat cattcagaca atgactgtag tgatggaacg ccgacaataa
    43501 tgtgccgtct ccgccgactg agatgaccaa ttctggctca tgatcatctc gttgaatccc
    43561 tgcttgttct aaaagtgcgt tcaaacgttc tgtcacttct atagtctttg cttctttatt
    43621 atgaacgatc gctactttca tcccctgcct cctctctttc gctttctttc ttcaaaagcg
    43681 ttcgatagta gacatcatct gattttcctc ttccatggga gaataaccgc tgtgcttctt
    43741 ggatttcttc acggatactg gacatctctt catccaactg ataggcagct tctgccgcac
    43801 gctgaagtcg ttcactcatt tcttctggaa aaacgccttg atatttataa ttcagcgaat
    43861 gctcaatcgt tgcccagaaa ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt
    43921 tcttctcacc gctgatcagc tgtacaggat actcgatcac cagatgataa gaacggtagc
    43981 cacttgcttt tttatttgtg atgtaatctc gttcttggag aactttcatg tcttttcttt
    44041 tacggatgat ctcaacaact tgatggatat cttctacaaa ctgacacatg atccttaacc
    44101 cggcaatatc agacatttct tcttctaaac gtcccattgg aatatgccgt aattgtgatt
    44161 ttgttacaat actatcgact ggcttgactc tcccagtgac aaattcaatc gggacatgtc
    44221 gattttgttc acggaactgt ttgcggatcc cccgcagttt cactttcaat tctgatacag
    44281 tttgttcata tggtgctaaa aatgcttccc aatctcgttc catagctgat acctctactt
    44341 cattaacatt cattgtttat tctaccatat atgcaaagtt ttttcgtact cttgatttat
    44401 gcattgacca aaaaaagaaa attaggcaaa atagacaaaa aggagtcgat caaatgacac
    44461 aaatgcttga aatcgaatat aaatctatgc tgacaaagca agagtacgag atattcatcg
    44521 ctcactatca attaacagat aaagatttcc gtgtacagac taatatttat ttcgataccg
    44581 ttgatgaaca gctgaagaaa caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag
    44641 ctgaatatac attaaaaaca cctgctgaaa aagggcgtct cgaaacaacg gataccttta
    44701 taacagaaga agcagaagca tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt
    44761 ttcaaaaact ttctgatttg aatatcgatc ctgcttcatt aatccaaacc ggaaagctta
    44821 caacaaaacg ggctgaattc cctattgaag aaggactttt agctattgat gaaagctggg
    44881 gagacaatct acacgatttt gaactggaac tagaggtaag gggatgcatc tgtgggaaaa
    44941 attgctttta taaatttttt aaaacgtttt tctttacctt atcgtcctgc taagaacaag
    45001 atccaacgta tgcttgaagc taaaaattag aaacctttca ttttcacaag gcacttgagt
    45061 tcattttttt acttttttct gttaagttat aggatgtgaa agaatctagt cactagttgt
    45121 atagaatcta ttttggggga gattcatatg atcgaaatct atttatttgt taatccttta
    45181 ggggaatact gtttcgaaat ggaacaacag ttattacaat ttatcgaaga agagtacggg
    45241 acaccatcaa aagaaaagat gcaatttcgc tttcttcctt tggtcaattt gcagacaatt
    45301 ggggatgtca tgcaacaaaa aggaatttct cagaacaatc ttgaggaacg taatcactta
    45361 ttctcaatga cttattctgc agcattggac tgcaaagcgg cgcagtttca aggaaagaaa
    45421 aaaggccgcc gttttttgat caaattgcaa gaagcagtcg gctgtaatga cataccatat
    45481 tctcaagaac ttgctgaatc catatttgaa gaaatcggcg gcgatttgaa tatgtttaaa
    45541 gaagatcgtc agtctgactt cgttaaagaa gtattctttt ctgatcaaaa aattgctcgg
    45601 gaaatgggca tcacaaaaca cccttctgca gtcgtttaca attatgcctg cgaccgtgat
    45661 tttggtgttt tagtagaagt aaaaggtaca agtagccttt ggagaatcac agagctgtgt
    45721 aaaacaacag ataactcata tcaagttttt aatggtgaac atttacatac aaatgattct
    45781 cagttccatt caagaactag ccatttacac ttgctttcta attaaaaaaa tacgtcgact
    45841 cccttacaag agagccgacg tatttttttc gtttctattc gagaaaatca aaaatagaaa
    45901 gccttataaa cgatcgatca attcttctaa ttcatttaga cgttgttcaa acatcgacat
    45961 tgcatcttca atatatgcag cctgtgtcat atcaactcct gcttttttca ttacttccac
    46021 aggataatcg ctgttacctg cttttaggta agccaagtag ttctctagtg cctctggttc
    46081 ctggtttagt attttcttcg ccagtgctga agcagcagaa aagccagtcg agtattggaa
    46141 aacatagtaa ttataataaa aatgcggtat ccgtgaccat tcgaatttaa tttctgggtc
    46201 ttcttcgact gctggaccat aatattttgc attcaattta ccataactat cacttaggta
    46261 ttcactagtc aatggcacac ctttttcatc ttccgtatgc atgaaatgct cgaattcagc
    46321 gaattgtgtc tggcggaaaa ctgttccttt aaacccatcc aagtaatggt tgagtacata
    46381 agcccgtaca cgagggtctt tttctgtttc caataaatac tccgttagga tattttcatt
    46441 cgttgtcgaa gcaatttctg ctaaaaagat ggagtagtcg ccgtacacat aaggctggtt
    46501 cgaacgagtg aaataactat gaacactatg tcccatttcg tggacaagcg taaatagctg
    46561 atccagcgta tcatgccaat tcaataaaat atatggattt gtgtcatagc ttcccgaaga
    46621 ataagcaccg cttcgtttcc ctttattttc gacaacatcg atccaacgtt cagagaatgc
    46681 tttttctacg atggccatgt attcttcacc cattggtttc agtgcttcta aagctttttc
    46741 ttttgcttct tcgtacgtaa aggtaattgg agcttcccct aagaccggtg tataaagatc
    46801 atacatgtgc agtttttcca cttctaataa acgtttccgt aattccatgt atcgatgtaa
    46861 caaaggcaaa tgcttgttta ccacgtctac caaagtatcg tatacacttt caggaatatg
    46921 attattgctc aaagacgctt ctctggcaga gctgtaatta cggactttcg ctttaaaatt
    46981 atgtcctttt atatgtgtgc ctaaagtaga agcaaatgta tttctaaatt gttcgtaaac
    47041 actgtacaat cccttaaatg ctgcttcgcg cacccttcga tccgtgcttt ctagcaactg
    47101 accatacacg ccatgagata attggactat ttcaccattt tctccttcaa tcgttggaaa
    47161 aactagatct gcattattca aaacagcaaa tgtatcactt gaagcatcaa agatttcacc
    47221 tgctccagca agaagagatt cctgctcagc agataagaca tgggctcgat tatctactat
    47281 ttgctggata taatggcgat agacttccaa ttttggttct tctttgaaat attgccaaat
    47341 ctggtcatct gacaattgca atatttctgg ttcaaaccag gaaacggctt cactaacttt
    47401 cgaaaacaga ctgcttgctc ttgcataaag cgcttggtaa tctgtatttc cagtgtcttg
    47461 atcgttttta aggtgcgcat atacataaat gacttcagtt tggcgataaa ccctcaatac
    47521 gaattcaatc gcatttaaaa attgagaagc gccttgatca agtgttcctt tgtgtttttc
    47581 agattgtttt aactcttctg ataattccaa atatttctca tcaaactctt ggtcgcttga
    47641 aaagatcttg gtcaagtccc aagttaaatt ttcaggcaat tcttctcgtt ttggcaactg
    47701 ctttacttcc atgaaatcca ctccttttct tcccttagct ctatctatca aaatagatca
    47761 tagtctaaca tccacatgtc acagcatcta acttttctac tcattcatgt gtctgcttcc
    47821 atcaatttta gcataactaa atcttgtttt acatagaaaa ccgattccta gcctattttt
    47881 atacttacta ttttttcaaa gaagacaagt gtatgcattc gttaaaaaac tgctgataga
    47941 ccatctcttc atcaatcata ggaaaaagcc attccctttt atgttctttt atttggcaaa
    48001 gccatgagtt gaaatcagga actttctggt tttccaaggc ttcttcgtac aacattcgat
    48061 aaagaaaaac ctgttcttgt aaataaaaga aaaaccgact tttttgatag atccacgatg
    48121 atagatgcaa aagatgttta tgacgcaagt agcattgttc ttgtatggat actgtattct
    48181 tttgcttgtg aataagttgg cgtgtcagcc atttcttccc atcttcactc actttttcta
    48241 tttttttcat ttggttcatt ttaatttcgt tactagtgaa tatttcttta aggtccctac
    48301 tagaaaaagt ccagcagcta ctgcaaaagg aaatatgccc tgatacagtc tctgtaatat
    48361 gatgatacaa ataaagcttt tgtttcttga gatcagcttt ccagcaatgc actcctcttt
    48421 ttctattata gtagcagcaa cttttttcta tcaatgagaa ttgcgattgt ccaagaaaag
    48481 aattaccaag tatccaccat acttggtaac tgttattcaa ataattttgg gttctttcaa
    48541 taaatctctg gtgagacagt ctagaacatt ggatttctat tgctagattc gccaacaaaa
    48601 tatccggcct ttgccgcaac ctcggcaaat aggcttccaa aaatactggt tctactgact
    48661 gtcccagcca atccatgaag cattccttta actgtaagtg ctcttttgat tctctttcac
    48721 taaagaccga acagttatct gatttttgat gggcaaaatg agcacatttt ttctttccct
    48781 ttttcaaaat cactcttttt cgacagccac gacaataata tttctttcct ttttctgcat
    48841 gtttagcaaa acagcactta ttatattcat tcattgcaag cagcattttt ctccctctga
    48901 acttatttac gtctaagaat ttatttacac gaaaaaagag ggcgtgaaaa agctattctg
    48961 catcaagtaa taagaacagc taaacaaaaa tagttcccaa tatttttcgt ttggttgggc
    49021 ttgttaccgc agatgcaagc ttttgaacac atttatacga aaaaaagact gcgacacaat
    49081 ttttgtcaca gtctcttact ataatgctga tttatacaaa ataaaatttc gttcgctcga
    49141 tagcattacg cgccatcaga cattgtccat attcattcaa tgtttctggt gtacgatcgc
    49201 tcttttcagc aaactccaaa attcgagcaa tctgattctc tacatccgct tggttgagat
    49261 tttccatcca gaagtgaacg actaggtaat agcgctcttc gaataaatac aaatcagtcc
    49321 aagcagactc tagttcgaca tctgttgcaa gttggatcac ttgttcaaaa tcttcaaaag
    49381 caaaagttgc ttgtctttcc tgttcttcta gggcgcttcc agataattct tcctccggat
    49441 cgctgccaat aatctgctga cgaagaaagt cactcacatc ttctgaatca acagtagaat
    49501 cttctgattg agaaatttct tcatccggaa gatttttact gataaacagt tctaatccgt
    49561 ctccttttgg caaaacttgg aaagtgacag cttcgcttcc tcggaactcc tcttcgatat
    49621 caacttcttc caaaatacta taaaagaaat tttcgatttc tttgtgattg ccaagtaaat
    49681 ccaaaaatga aacgccccgt tcctctaaat cttcatgacc aattagtaca cggatcgtat
    49741 tttcattaat atgttccatt tccattttgc tacacctcac tttgccacaa tgtttaatat
    49801 agattcattg tagcgaaatc agcttaaatg taaagaaaaa aaatctcgct gttaagcgaa
    49861 tttaataaaa ccagcggatt tttcaacctt tcaagctatc ttatttaact ataacatttt
    49921 tggcagacga atactatata gccgactttt cagctaattt tccctctcta tttttcgtgc
    49981 aatcatcatc ttctactacc tttttataca tctaatcaag aatacttttt gtaaaaacaa
    50041 aaaaacttgg aaaaactatc tttgtaatag tctt
//
LOCUS       DASJUF010000006        49991 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJUF010000006 DASJUF010000000
VERSION     DASJUF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.006"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            370..3177
                     /locus_tag="U4B62_000323"
     CDS             370..3177
                     /locus_tag="U4B62_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2140509.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3368..3859
                     /locus_tag="U4B62_000324"
     CDS             3368..3859
                     /locus_tag="U4B62_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2140510.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3962..4951
                     /gene="manX"
                     /locus_tag="U4B62_000325"
     CDS             3962..4951
                     /gene="manX"
                     /locus_tag="U4B62_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN2140511.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4984..5787
                     /locus_tag="U4B62_000326"
     CDS             4984..5787
                     /locus_tag="U4B62_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN2140512.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5808..6722
                     /locus_tag="U4B62_000327"
     CDS             5808..6722
                     /locus_tag="U4B62_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN2140513.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6805..7179
                     /locus_tag="U4B62_000328"
     CDS             6805..7179
                     /locus_tag="U4B62_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN2140514.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7318..7563
                     /locus_tag="U4B62_000329"
     CDS             7318..7563
                     /locus_tag="U4B62_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2140515.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7735..8799
                     /locus_tag="U4B62_000330"
     CDS             7735..8799
                     /locus_tag="U4B62_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2140516.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9045..9470
                     /locus_tag="U4B62_000331"
     CDS             9045..9470
                     /locus_tag="U4B62_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2140517.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9463..9912
                     /gene="nusB"
                     /locus_tag="U4B62_000332"
     CDS             9463..9912
                     /gene="nusB"
                     /locus_tag="U4B62_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN2140518.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10009..10863
                     /locus_tag="U4B62_000333"
     CDS             10009..10863
                     /locus_tag="U4B62_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN2140519.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10864..12201
                     /locus_tag="U4B62_000334"
     CDS             10864..12201
                     /locus_tag="U4B62_000334"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN2140520.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12206..12430
                     /locus_tag="U4B62_000335"
     CDS             12206..12430
                     /locus_tag="U4B62_000335"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN2140521.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12432..13322
                     /locus_tag="U4B62_000336"
     CDS             12432..13322
                     /locus_tag="U4B62_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2140522.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13331..14149
                     /locus_tag="U4B62_000337"
     CDS             13331..14149
                     /locus_tag="U4B62_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN2140523.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14256..14705
                     /locus_tag="U4B62_000338"
     CDS             14256..14705
                     /locus_tag="U4B62_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2140524.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14723..16405
                     /gene="recN"
                     /locus_tag="U4B62_000339"
     CDS             14723..16405
                     /gene="recN"
                     /locus_tag="U4B62_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN2140525.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16460..16576)
                     /locus_tag="U4B62_000340"
     CDS             complement(16460..16576)
                     /locus_tag="U4B62_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN2140526.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16649..17605)
                     /locus_tag="U4B62_000341"
     CDS             complement(16649..17605)
                     /locus_tag="U4B62_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2140527.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17776..18144
                     /locus_tag="U4B62_000342"
     CDS             17776..18144
                     /locus_tag="U4B62_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN2140528.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18314..18745
                     /gene="mraZ"
                     /locus_tag="U4B62_000343"
     CDS             18314..18745
                     /gene="mraZ"
                     /locus_tag="U4B62_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN2140529.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18766..19725
                     /gene="rsmH"
                     /locus_tag="U4B62_000344"
     CDS             18766..19725
                     /gene="rsmH"
                     /locus_tag="U4B62_000344"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN2140530.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19730..20131
                     /gene="ftsL"
                     /locus_tag="U4B62_000345"
     CDS             19730..20131
                     /gene="ftsL"
                     /locus_tag="U4B62_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN2140531.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20132..22324
                     /locus_tag="U4B62_000346"
     CDS             20132..22324
                     /locus_tag="U4B62_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2140532.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22350..23312
                     /locus_tag="U4B62_000347"
     CDS             22350..23312
                     /locus_tag="U4B62_000347"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN2140533.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23326..24687
                     /locus_tag="U4B62_000348"
     CDS             23326..24687
                     /locus_tag="U4B62_000348"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN2140534.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24713..25801
                     /gene="murG"
                     /locus_tag="U4B62_000349"
     CDS             24713..25801
                     /gene="murG"
                     /locus_tag="U4B62_000349"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN2140535.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25816..27036
                     /locus_tag="U4B62_000350"
     CDS             25816..27036
                     /locus_tag="U4B62_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN2140536.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27192..28517
                     /gene="ftsA"
                     /locus_tag="U4B62_000351"
     CDS             27192..28517
                     /gene="ftsA"
                     /locus_tag="U4B62_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN2140537.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28540..29781
                     /gene="ftsZ"
                     /locus_tag="U4B62_000352"
     CDS             28540..29781
                     /gene="ftsZ"
                     /locus_tag="U4B62_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN2140538.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29798..30475
                     /locus_tag="U4B62_000353"
     CDS             29798..30475
                     /locus_tag="U4B62_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN2140539.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30489..31088
                     /locus_tag="U4B62_000354"
     CDS             30489..31088
                     /locus_tag="U4B62_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN2140540.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31103..31390
                     /locus_tag="U4B62_000355"
     CDS             31103..31390
                     /locus_tag="U4B62_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN2140541.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31439..32221
                     /locus_tag="U4B62_000356"
     CDS             31439..32221
                     /locus_tag="U4B62_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN2140542.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32242..32949
                     /locus_tag="U4B62_000357"
     CDS             32242..32949
                     /locus_tag="U4B62_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN2140543.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33211..36009
                     /gene="ileS"
                     /locus_tag="U4B62_000358"
     CDS             33211..36009
                     /gene="ileS"
                     /locus_tag="U4B62_000358"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN2140544.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36058..37584)
                     /locus_tag="U4B62_000359"
     CDS             complement(36058..37584)
                     /locus_tag="U4B62_000359"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN2140545.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37599..38246)
                     /locus_tag="U4B62_000360"
     CDS             complement(37599..38246)
                     /locus_tag="U4B62_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN2140546.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38430..38759)
                     /locus_tag="U4B62_000361"
     CDS             complement(38430..38759)
                     /locus_tag="U4B62_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN2140547.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38936..39664
                     /gene="sfsA"
                     /locus_tag="U4B62_000362"
     CDS             38936..39664
                     /gene="sfsA"
                     /locus_tag="U4B62_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN2140548.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39680..40693
                     /locus_tag="U4B62_000363"
     CDS             39680..40693
                     /locus_tag="U4B62_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2140549.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40693..41970
                     /locus_tag="U4B62_000364"
     CDS             40693..41970
                     /locus_tag="U4B62_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2140550.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42033..44735
                     /locus_tag="U4B62_000365"
     CDS             42033..44735
                     /locus_tag="U4B62_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2140551.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44887..45204
                     /locus_tag="U4B62_000366"
     CDS             44887..45204
                     /locus_tag="U4B62_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2140552.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45234..45554
                     /locus_tag="U4B62_000367"
     CDS             45234..45554
                     /locus_tag="U4B62_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2140553.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45662..47122
                     /locus_tag="U4B62_000368"
     CDS             45662..47122
                     /locus_tag="U4B62_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2140554.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47190..47411
                     /locus_tag="U4B62_000369"
     CDS             47190..47411
                     /locus_tag="U4B62_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140555.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47442..47624
                     /locus_tag="U4B62_000370"
     CDS             47442..47624
                     /locus_tag="U4B62_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN2140556.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47624..48037
                     /locus_tag="U4B62_000371"
     CDS             47624..48037
                     /locus_tag="U4B62_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN2140557.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48160..49341
                     /locus_tag="U4B62_000372"
     CDS             48160..49341
                     /locus_tag="U4B62_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2140558.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49412..49576)
                     /locus_tag="U4B62_000373"
     CDS             complement(49412..49576)
                     /locus_tag="U4B62_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140559.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49730..49803
                     /locus_tag="U4B62_000374"
     tRNA            49730..49803
                     /locus_tag="U4B62_000374"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49764..49766,aa:Arg,seq:cct)
BASE COUNT        16633 a         8454 c        10791 g        14113 t
ORIGIN      
        1 taaaagttgt agtggaggct acgaagttta ctttgtcttt ttacacttaa tatttttttg
       61 ccaatttgga atcaggatgt tttgataact atcgttttca attgatggaa gctatattgt
      121 ttgctatcag tgaaataatt atatggtaaa cagtgttctt tttcttgttt ccattataaa
      181 aatcgtatcg ggatttttcg ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc
      241 aacaaaacaa aatcaggtaa atagttgacg aaacagaaat aaatacatag tattatgttt
      301 tgaagcctaa gtacaatagt ctatagaaaa ggagcgatac cattggcaaa acgcattgac
      361 cgaatttata tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga atatgatcaa
      421 ggagtaacaa cacaggaaat tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa
      481 gatctgaatc agctagtgcg cgaaggaaaa ctgataaaga cagacggacg accagtacgt
      541 tacatatatc aaacactcgt tacgcacgta aagccagtta caaaacatgt agtcagttac
      601 aaagaaacgc cggtctctga agaaaaaaat acactccaaa tagatactaa ggatatcttt
      661 gcaaaaatca ttggagctaa cggcagcatg aaaaattcag tggaacaggc aaaagctgcg
      721 attttgtatc cgccaagagg attaaattgt ttgattacag gacctaccgg atcaggaaag
      781 acttattttg cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa
      841 acggaactga ttgttttcaa ttgtgctgat tatgccaata accctgagct tttgatgagt
      901 catttatttg ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa aatggggatt
      961 atcgatcaag cggatggcgg tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa
     1021 ggtcaagaaa tgatctttta ctttatggat catggaactt acagtcgtct tggggaaaca
     1081 acgaaatccc atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga cccaggttct
     1141 tctctattag aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa
     1201 aaacgtcctg caaatgagaa agtcgattta gtaaaaatca tgattgccca cgaagcaaac
     1261 cgcacgcagc gaaagatttc tttgacagaa gatgtagcaa aagcattgat cggcagtgtg
     1321 acctatggaa atataggaca attgaagtca aatatccagt tagtatgtgc acgaggattt
     1381 ctaaatcata tgcaatcacc cgaaatctcg atcacaatcg atgatttgac agaagggatt
     1441 cgtagcggat tgattcaatt agcaagcaac cgcagtgcaa tggcagagct ttctaaaata
     1501 ttggagccaa agataactgt agcaccaaat gatacgatca tgaaaatcca atcggactcc
     1561 tatgagttac cttataatct atatgatatc atcggagata aagcagcttt actgaagtcg
     1621 gatggtttgg atcaagaagc aatcaatcat ttcatttcta cagatatcaa catccatctg
     1681 aagtctttct ataaagacca tggtttttct tttaatgcag acagcaaatt agcagaattt
     1741 gtagatccga aaatcataga agtgaccaat cagatttatg taatggtcaa aagatcttta
     1801 ccatatgagt ttcagcaaaa ctttatttat gctatgagct tgcatatcag ttcttttttg
     1861 aaaaaaatca acttaggaga agaacggcat acgaatgata acatccgtga gatggctttt
     1921 gattttccag atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt
     1981 caagtggaga ttccagaaag tgaagactat tacttgaccg ttctactcgt ctcgttacgg
     2041 gcaaatcaag caagcggcag aatcggtgta gtcgttgcag ctcacggaaa cagcacagca
     2101 agcagtatgg tccaagttgt ccaacagttg ttagatgcag agaacgtacg agcagtagac
     2161 atgcctttgg atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa
     2221 gtagatgaag gaagcggggt catactctta gtagatatgg ggtcccttgc gtcgtttaat
     2281 tcgcaaattc aaagagacac tggaattcca gtacgtactg ttgatatggt aacaacttct
     2341 ctagttttgg aaactgtacg aaaggtctca gtattaggga cagatctaga tatgttgcat
     2401 gaatcattga aaaattttcg aggttacgca gaaatatctt ctgtagaatc tgttcaggaa
     2461 gcaaaaccta ttcataagaa aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca
     2521 gcacaaagaa tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga aacagaatta
     2581 gaagttctag ctttgtcgat tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa
     2641 tatcagatca tagctactac tgggattact gatttgaata tccaagcccc atttattcct
     2701 ttagaacgtt ttattgatca aaatatcgaa gtcattttgg atcagttgct gatggaatca
     2761 gaattggagg aaactgaatt catctcccta gatgaagagt cagctaaaaa tacgtgtgtt
     2821 gagtttatct ctgataactt catctttatt aatggtagta agttaatcga tccaatgtgg
     2881 caattttcta cacaaatctc tcagacgacg ggaatcggcg atgaagaata tggattcaaa
     2941 atcaacctag tgatgcacac agcaggaatg atcgaacgta ttattcggaa tgaaccattg
     3001 actgtggaag aaaacgaatt gacaaataca acgaatgatc ctctttatag ccaattggca
     3061 gcctcagttg ttctgttgga agatcagata aaagtaaaag tgccgataga agaaatgtat
     3121 tatctgctac gtctagtcca taatcagtta gataaaaaag aatacactgt gccatgaata
     3181 cacaaaaacg atacactttt atagtgtatc gtttttgtgt attggttaag aatcctttaa
     3241 attcaattag tgtatcgtaa aaaagttggc acgctttttg cttatagtag tgtgtgctag
     3301 gaggtggttg ttcacacaga aatgataggg gtttctgtga agtagtgagg aagaaggagt
     3361 tttgcagatg gatattcgat tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc
     3421 aacagtttgg tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg atgtagtcgc
     3481 tcaagataaa ttgagaaagt ttctactaaa agaagctgca ccgccgggga tcacagcaaa
     3541 tgtaataacc gtgcagaaaa tgattcaaat ctatcatagt ggattattat cacgagcaaa
     3601 agtgatgctt ttattcacca atccccaaga tgttgaagcg gttgtccgct gcggtgtaca
     3661 gttgaagtcg ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa
     3721 ttttgtttca gtaaatcaac aagatattga ttcctttcgt tacttgaaca gtcaagaagt
     3781 ggagttagag attcgaaaag tacctgctga caggaaaatc cttttgatgg aactactagc
     3841 gaaaatcaaa aaggtgtagc aagaatggtt gttggaaaag gtcaaataat tctgttgtaa
     3901 tggttagcaa ttattaatat ttttgaattt gttacaacaa aagtaaatag gaggtataaa
     3961 tatggtaggg attatcctag caagtcatgg acaatttgct gaaggaatct tacaatccgg
     4021 ttcaatgatc tttggcgaac aagaaaacgt caaagccgtg attttgaaac caagtgaagg
     4081 tccagatgac ttgagagcca aattggaaga agcggttgct tcttttgata atcaagacga
     4141 agtgttattc ttagttgatt tatggggcgg aacaccgttc aatcaatcaa acacattatt
     4201 cgaagagcac aaagataaat gggcaatcgt cagcggtttg aatttaccaa tgttgattga
     4261 agcctacgca tcacgctttt caatggaatc tgctcatgaa attgcagcac acatcattga
     4321 aacagcaaaa gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa
     4381 agcagcagtt gaagacgcac aacctaaagg agcacttcca gaaggaactg tggtcggaga
     4441 cggaaaaatc aaatatgtat tagcacgtgt tgactcacgt ctgttacatg gacaagttgc
     4501 gacagcttgg acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc
     4561 aaaggatgat ctgcgtaaga gactaatcga acaagctgct cctccaggtg taaaagcaaa
     4621 tgttattcct attagcaaaa tgatcgaagt agcaaaagac cctcgttttg gaaataccaa
     4681 agcattgctt ttatttgaaa atcctgaaga cgtattgaca gctgttgaag gtggcgtaga
     4741 catcaaagaa ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa
     4801 agtattgtct atgggacaag aagacgttga agcgtttgag aagcttgaac aaaaaggtgt
     4861 gaaattcgat gtacgtaaag tacctaatga ttcacgcgat aacatggatg atattttgaa
     4921 aaaagcaaaa gctgaattag caaaagcgta ataaataata ttaaaattta taggaggctt
     4981 atcatgtcta ttatttcaat aattttagtc gtattagttg cctttctagc tggtatggaa
     5041 ggtattcttg atgaatttca atttcatcaa ccactagtgg catgtacatt gatcggttta
     5101 gttaccggta acctcgaagc tggtatcgta cttggcggta cgcttcagat gatcgcttta
     5161 ggttgggcaa atatcggtgc cgctgtagca ccagatgctg cattagcatc agtagcatca
     5221 gcaattattt tagttttagg tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt
     5281 gccgttcctt tggctgttgc agggttgttc ttaacaatga tcgtacgtac aatcgctgtt
     5341 ccaatcgttc atttgatgga tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg
     5401 tggcacatca ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc agcagcgttg
     5461 ctatttatcc cagcacaaac tgtgcaatca ttccttgaat caatgccagc atggttaaca
     5521 gatggtatgg ctatcggtgg gggaatggtc gtagctgtag gttacgcttt ggtaatcaac
     5581 atgatggcta caaaagaagt atggccattc ttcgttattg gtttcgttgt agctgcaatt
     5641 tctcaattaa cattgattgc tctaggtgct ttaggtgttg cattagctct tatttacttg
     5701 aacctttcta aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc tggtgaccct
     5761 ttaggcgaca ttttgaatga ctattaatct tgaaggagga aaataaaatg gcagaagaaa
     5821 aaatcaaatt atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag
     5881 gttcatggaa ctacgaacgt atgcaaaacg gtggctgggc attttctatg attccagcaa
     5941 tcaaaaaatt atacaaaaca aaagaagaac gttctgcagc attaaaacgt cacttggaat
     6001 tctttaatac acacccatac attgcttctc caattttagg ggttacattg gctctggaag
     6061 aagaacgtgc gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt aaagtaggta
     6121 tgatgggtcc tttagctggt gttggtgacc cagtgttctg gtttacagta cgtccaatgc
     6181 taggtgcact aggtgcttca ttagctatgg gaggaaatat ccttggacca atcatcttct
     6241 tcttaggttg gaacattatt cgttgggcat tcatgtggta tacacaagaa tttggctaca
     6301 aagctggttc taaaattact gatgaccttt ctggcggatt gttacaagac gtaacaaaag
     6361 gtgcttcaat tttagggatg ttcgtattag ctgcccttgt acaaagatgg gtatcaatca
     6421 aattcttacc agttgtatca actgtaaaat tagataaagg tgcttacatc gaatgggata
     6481 aattgccttc aggtggtgaa ggaatcaaga gtgcatttga acaagttaat aatggcttag
     6541 ccctttctcc tgaaaaggta acaacattac aagacaactt agaccaattg attcctggtt
     6601 tagctgcatt aggattaaca ttgttatgta tgtggttgct taagaaaaag gtttctccaa
     6661 tcgttatcat ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat
     6721 aatccataaa aatatagctc gggcgttgtc ccgagctttt tttatatagt atactgttca
     6781 taaacaaata tctggaggga gaaaatggtt caatcaatca atacaaaagt agacttggta
     6841 attgatgcta cttcgtttac gggtctgaca gattacgggc aaatcatgat aggtaataat
     6901 ggttttgagt tttaccatgc gcgagataga cgcaaatata tccaaattcc atgggaagaa
     6961 gtggattatg tactagcatc agtcatgcta aagggaaaat ggattcctcg atatgccatt
     7021 caaacgaaaa aaaatggtac atacacattt tcttccaaag aacctaaaaa agtgcttcgt
     7081 gcgatcagag aatatgtaga tgaagataga atggtacaat cattgagttt ctttgatgta
     7141 gtgaaacgtg gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag
     7201 aggaggtttt ctttattttt ttactttgca gaagaaaata tgaaatagca aaaccaatta
     7261 tttgtcccct ttaaatccca tatgctaaaa ttgattagca aagaaggagg ttatattatg
     7321 agttggttat ggacattgat tgttggagcc gtgatcggag caattgcagg tgcgatcacc
     7381 agtaaaggaa attcgatggg ctgtgtaacg aatatcattg ctggactagt cggttcaagt
     7441 attggacaag cgattttcgg cgaatgggga ccaaaagctg caggtatggc acttattcca
     7501 tctattttag gcgcagtgat tttggtagca gttgtctcat ttttctttgg aaaacgatca
     7561 taagttttta caaaatagta aactaaaagg agttgcagcg agactgtgac tcctttttgg
     7621 ctttatttat ttaaaataaa aagaagtttt ccggaaattt ttatttaact gtcgtgtacg
     7681 aagaaaagtt tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg
     7741 ctacgtgtag aaaaattgcg caaaaaaatg caagaagaaa atttggattc atttttggtt
     7801 accagtccgt acaatcttcg ttacttaacg aactttacag gtacgacagg attagctgtt
     7861 attactttgg aaaaagcatt ttttattact gattttcgtt ataccgagca ggcggcagcg
     7921 caggctcaag ggtttgaaat tattaaaaat gtcggaccta tttttgatga ggtagcaaac
     7981 ttagtacgca aagaagaact gcactcatta gcttttgagg aaacaacagt ctcattttta
     8041 gaatattcgg ttttagaaga aatcatcgat gcggaattag tacctgtttc acagatgatc
     8101 gaagaactca gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat
     8161 attgcggata tggcatatga tcacatcctg aaaatgatcc aaccgggcat gactgagatt
     8221 gaagtcgcaa atcaactaga cttttatatg cgttctcttg gcgcttcagg tgtttctttt
     8281 gatacgatcg ttgctagcgg attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa
     8341 atcatcgaac aaggtgactt gatcacattg gactttggtt gctattacga agggtacgtt
     8401 tccgatatga cacgtacatt cgctattggt gatcctgggg aaaaattaaa agaaatttat
     8461 caaatagtct tagatgctca attagcagtt attgatgctg caaaaccagg aatgactggt
     8521 gtccaattag atgcagttgc tagagattat atttcaaaac atggttacgg ggaagcattt
     8581 ggacattcta caggacatgg gatcggttta gagatccacg aaggaccaaa cgtttcttct
     8641 cgtgcagaaa agcaatttgt accaggaaat gtgatcactg atgaaccagg gatctactta
     8701 ccaggaatcg gtggagtacg tattgaggat gacttgttga tcacagaaga tggaaatcgt
     8761 gtgttgactc gttcaccaaa agaattgatc atcttatagt ttcatcaata gaagaagtaa
     8821 gcgtaaaaat cagtgttcac agactttgtt atagaaaatc actcgattta atgatacact
     8881 atagaagaac ttttaagctg taagagataa taaagttggg tgttttctcc aattcatagc
     8941 agtttgcagc tgatcggcgg cagttttctt accacgtgat cactatgccg catcatagag
     9001 aattcggatg tatctcgtgc ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa
     9061 atttgatttt aaacgcaaac caagatttag gagagatcgt gatcgctcct gaagtcatcg
     9121 aagtaattat cgggattgca gcttctaaag tagaaggcgt atacggaatg cgcggaactt
     9181 ttgccagcaa tgtcactgaa ttattaggac gtgcagctca cggaaaaggt gtttacttag
     9241 caaacgagga agatggcttg aaagtagatc tttactgtta tctagaatat ggtgtttcag
     9301 ttcctaaagt agcaatggaa atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg
     9361 atatcgattt atctgaagtc aatatccatg tagtagctgt tgttcctgaa aaattagaac
     9421 aaccaacaat cgaagaactt tttgaagacg aagaggaaga gaatgaataa agaattatca
     9481 agacacgaga ttcgggaaat ggcattgcag gctttatttc cattagactt caatgcagac
     9541 ctaacaaaag aagatgcgat tttcaacgca atcgaattag accaccgcga catgatcaac
     9601 gaagatgaat cagagtttgt tcctgtttat cttgatacgc tagttggcgg tgtctgtgca
     9661 aaaaaagatg aattggataa agtgattgaa aaacatttaa aaaacaattg gcacatcaat
     9721 cggatctcaa aaatggattt ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg
     9781 acagatgtac cagcggctgt tgctttaaat gaagcaatcg agttggcaaa aacatttagt
     9841 gatgaccgtt cacgtaaatt cgtcaacggt gttttatcca atgtattgaa agaattagaa
     9901 gctggggcgt aagtcctagc ttttttctat gaatcatcat aaacctgtta taaatcggga
     9961 aaattctatg gtagaatgaa aacacaaaaa atatctggga gtggttagat ggcagaattg
    10021 attaacggaa aagaattagc tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa
    10081 ttgaaagata atggtatcca tcccggatta gtcgttttat tggtagggga aaatccagcc
    10141 agccaaatct atgtaagaaa taaagaacgt gtagcaaaag aaatcggaat ccattcattg
    10201 gtagagcgtt atcctgagac gatttctgaa gacgaactac ttgcagaaat tgaaaaatac
    10261 aatcaagatc ctagttttca tggtatattg gtccaattac cattgccaaa gcatatagat
    10321 gaagaaaaga tcctgttagc tatccgccct gaaaaagacg tagacggttt tcatccgatg
    10381 aatttaggca gactccttgc gggtaaaccg gacaagattc cttgtactcc atacggcatc
    10441 atgaaaatgt ttgaagctta ttctatcgat ccggcgggta aacgagcagt agttatcgga
    10501 cgaagcaata tcgtcggtaa accgatggct cagctactct tgatggcaga tgcaactgtt
    10561 actgttgctc attctaaaac cgaaaatctg gctgaactgg cacaagaagc agatatttta
    10621 gtcgtagcaa taggacgtgg acattttgtc acaaaagaat tcgtaaaacc aggtgctgta
    10681 gtgatagatg tagggatgaa tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt
    10741 gatgaagtgg agcctattgc aggctggatc actcctgtgc caaagggtgt agggccaatg
    10801 actatcacga tgctgatgta tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa
    10861 tagatggcac aagaatattt aactgtcact acattaacga agtatttgaa aagaaaattt
    10921 gatgccgatc cttatttaga acgcgtatat ttaacaggcg aaatatcgaa tttccgttta
    10981 cgtgccaatg cccatcaata tttcagctta aaagatgacc atgcaaaaat atctgctatt
    11041 atgttcaaag gagcattcca aaaattacga ttccagccaa aagaaggaat gaaagtcctt
    11101 gtcgttgggc gtatttcatt atatgaagcg ggaggttctt atcaaatcta tgtagagcac
    11161 atggagcctg atggaatagg tgctttatat caagaattgg aagaacgaaa agaaaagctg
    11221 atgaaggaag gcctttttca agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc
    11281 gcggtcctta ccagtccaag cggagcggtt atccgcgata tcatcacgac ggtgaaaaga
    11341 agatatccaa ttgtccaatt ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg
    11401 gacaatgttg tcaggaatat ccagagagta gaagagatgg gcaatttcga tacaatgatc
    11461 atcggtcgtg ggggtggttc gattgaggat ttatggccgt ttaatgaaga gcgggttgct
    11521 cgggcgattt atcaagctaa tacgccggtg atttcatctg taggacatga aacagatacc
    11581 acgatcgctg atttagtagc agatgttcgg gctgccactc caacagcagc tgcagaatta
    11641 gctgttcctg ttttgtcaga agagttgatg cgcattcaag aacggcaagc tagattgcag
    11701 caggcgtttg tacgccagat ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc
    11761 tatgtatttc ggcagcctga aagattgtat gaaggtcaga ccatcaaatt agaccagttg
    11821 aaacagcgat tatatcaagc tacacagcaa atttatcatg cgaaagaaaa acaaacgatc
    11881 gcattaagcc accaattgga acaagtggct ccgctttatc gagttaaagc agctcgccaa
    11941 gaaactgctt atctagaaaa acgcttagaa gagaaaatcc aacaatatat gaaacaacag
    12001 cagcagcgtt ttgctcaagc tgttcaatct cttgatttgt tgagtccatt gaaaatcatg
    12061 ggaagaggct acagctatac gacgttaaat gatcaagtag taaaaagtgt caccgagatt
    12121 catcaagggg atttgctgac gatccattat caagatggaa tcgttaaagg acaagtacaa
    12181 gagatagaca aaggagaata aaaatatgga aaatccaact tttgaagagt cactgcaaga
    12241 attagaaaaa attgtcatgc agttagaaca aggagacgtt ccattagaat cagctctaga
    12301 ttcgtttaaa cgaggaatgg aattaagcaa acattgccaa gatacattag ctaaagcaga
    12361 agagaccttg acgaaaatga tgacagaatc aaatgaagaa gttgattttg acggaaacga
    12421 ggaaagctga gatgagatta actgactttt cagctattca tctacctgct gtagaaaaag
    12481 aaatcttgtc ttttttagat gagcatacaa cggatagagg actctatgat gcgatgagtt
    12541 attcagtgaa agcaggcggg aaaagaatac gtcccttatt acttttaacg gccgttgctt
    12601 cctttgatga gcctattgat gttcccgtct atcaagtagc agctgcttta gaaatggtgc
    12661 atacgtattc cttgatccac gatgatttgc ctgcgatgga caatgatgat cttcgtagag
    12721 gaaaacctac caatcataaa gtattcggcg aagcattagc tatattggcc ggagatggtt
    12781 tgcttacagg agcattccag ctaatcagta tggcacactt aggtaattct cctaagcttc
    12841 tgctgcttca gcaattagct gtttgtgcgg gatcacaagg tatggttgct gggcaagctg
    12901 cagatatcga gggcgaatcg aaaaagcttt ctttagaaga attagcattt attcatgaaa
    12961 gaaagacagg gcatttgatt cgttatgcct tgctggctgg tggcatatta gcgaagcaac
    13021 ctgaagaaat cttattgcta cttcaacgat tagccgaaca tttaggttta gcttttcaga
    13081 taagagatga tctactcgat gtgattggta caacgaaaac attagggaaa acagctggta
    13141 aagatgaacg tatggaaaaa aatacttacc cgcgtttatt aggcctagaa aagacacgtg
    13201 aggcgttgga gattgagctg ttatccgcca acaagatcat agacaaatta gaagaggacg
    13261 ttttttcttt tgatggcggg ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat
    13321 gagggaaaga atggaaaaag aacgcgtaga tgtattagcg gtcaaacaag gattatttga
    13381 aacaagagaa caagccaaac gttcagtgat ggctggtttg atttacaacg aaaaaaacga
    13441 gcggtttgat aaaccgggag aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa
    13501 aaaacttcct tatgtttctc gagggggatt aaaattagaa aaagcactca aagcatttga
    13561 tctatctgtt gaaggaaaaa cattgttaga tattggtgca tccactggcg gatttactga
    13621 tgcagctttg caaaatggcg caaaaatgag ttatgcttta gacgttggct acaatcagct
    13681 agcttggaaa ataagacaag atcctagagt cgtcgtaatg gaaagagtga attttcgtta
    13741 tgcaaaaccc gaagacttta ccttaggaat tccagaagtt gctgttatcg atgtttcttt
    13801 tatttcacta aaactgatgc tgcctccatt acatgctatt ttaaaagaag agggagaagt
    13861 gattgcttta atcaaacccc aattcgaagc aggcagagaa gctgtaggga aaaacggaat
    13921 tgttcgtgac cctcagaccc atcaaaaagt tttagaagac attttgtcat ttgcggcaca
    13981 aggtggatat gatgtgctgg aattaagtta ttctccgatc acggggggag aaggaaatat
    14041 cgagttcttg gctcacttga ggaaagtacc cgaaagcgga acaatcaatt cagctattaa
    14101 tatggcagaa gtggtttcaa atgcacacga acagtttgac cataaataaa ttgtaactgt
    14161 gaaaatcaag tgagaagtat gcggattctt ctttattata ccaaggaaat aagctatcat
    14221 tgattaagaa gaatatgcta aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt
    14281 tgattactcg attgttgagt gagcaagata tccgaaagca ggaagaattt gttgaaatcc
    14341 tgaaaagcaa aggaatatct gttactcaag ctacgatttc cagagatata aaggaactga
    14401 aactgatcaa ggtaccggct tcagatggcg gctaccgtta tagcatacca gctgaaacaa
    14461 acgaagatgt aaatactaaa ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc
    14521 aaatggaaaa atttgttatt ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt
    14581 tgattgataa acgttacaag aaagaattat tttcgattat caatgatgac gataatgttt
    14641 tgatgattac acgaactgaa gaccaggcag ttttattgaa gaaagacttc ctgcattacc
    14701 tttaagttga ggagcgaatg tgatgctaca agaaatcagt attactaact ttgcgattat
    14761 tccggaattg cggctttctt ttcacgaagg aatgaccgcg ctgaccggag agactggtgc
    14821 gggtaaatcc attattatcg atgctttagg tctgttagca ggaggaagag gttcaagcga
    14881 ttatatacgt caaggagcag aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca
    14941 agaaggattc tctgaactga tggttgaatt agggatcgaa acagatgagg acaatctgat
    15001 tgttcgacgg gatatgtcct taactggaaa aaatgtttgt cgagtgaatg gtcatattat
    15061 cacattagct aatctgagaa gaatcggcag ttatttagta gatatccaag gacaaaacga
    15121 acatcaggaa ctgctccagc ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt
    15181 agctttccaa cagaagaaaa agagttatca gcaagaatat atctcttatc gtgagctgga
    15241 aaaaagggta cgaaaagtgc agcaaaatga aaaatcctat gttcagagaa tagatatgct
    15301 ccatttccag caagaagaaa tagctgctgc tgatttgcaa gtcggagaag aagagaaatt
    15361 aaaagaagaa agagaaaaac ttagcaatta tcaaaaaatt gctgatggat tagctgcagg
    15421 ttacggagca ctgacgaata atgaacaaaa cagtgtagat ggtgttggct tggccgtttc
    15481 agaactgcaa gggatcgccc atctagatgt ggagtatgaa gcaatttatg aaaatatcca
    15541 aagtgcttac tatttgctgc aagatgcaat cggcgatatg agtcgtcaga ttgatttatt
    15601 ggagttagat gagagtcgtt tagaagaagt tacgcaacgt ttagaattaa tccgacagtt
    15661 gaaacgtaaa tacggcgaat ccattgagtc aatattggct tactatgacg aaatcaccga
    15721 agagctagct tcttctgatt tttcagaagg tcaattggac aaaatgaaag aggaactaga
    15781 gcaaaaagag cttctacttc agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat
    15841 cgcaaaagaa ttggaaaaat ccattctgca tgaattgaaa agtctgtata tggaaaacac
    15901 tgagtttgag gtacgtttct taaaagaaga aaatagacaa ttgaatcatg atggatttga
    15961 ccaaatcgaa ttttacatta cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt
    16021 cgcttctggt ggcgaactgt ctcggatgtt gcttgctttg aaaactattt tttcttctga
    16081 gcaaggtgtg acaagtatta tttttgatga agtggataca ggtgtgagcg gaagagttgc
    16141 acaggccatc gcagataaaa tttctcaaat atcgaaatac tctcaagttt tgtgcatcac
    16201 tcatctgcct caagttgctg cagtcgccga ctatcaatat tatattgtga aagcagtgat
    16261 tgatggacgt acgcaaactt ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat
    16321 tgcacgtatg ctggctggaa gtgagattac aaaactcaca ctcgaacatg caaaagaact
    16381 attgaaactc gcaaaaagga cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc
    16441 tgatagctat attttatttt tacatgacac tcattacagc agtcaataca acagatccaa
    16501 tcgttaaaat cagcattgtc caaatgacaa attttgttac tttagcaaaa gtacttgttt
    16561 gttttttttc acgcattgtt ttcaacctcg tcattaatta gttgattcaa gtgtacaatc
    16621 ttttttccat ttccacaaga gggctttttt ataaaaactt cccttttttt aaataatagg
    16681 tagttacgat acaaataaat aatgttaata gaactagcca tataaaggca tcatcccttt
    16741 cagcaatagg taacttaatg ttcattccat agatactgcc gacgattgtc ggtatagtta
    16801 aaataatcgt taaagaagtt aaaattttca taatattatt cagattattc gatacgattg
    16861 ctgaaaaagt ctgattcatt tgttctacta gttttaattg aatgcgagaa gtagtcattg
    16921 cctgctctgt ttccaccaat acatcacgta aatgattttt caaagcatga ttttcttgga
    16981 aaagtttatt ttgcgctagt tctttcaatg catctaaatt agcagaagtc gcttctctga
    17041 aataaacgag gcttttttgg atatccatga tttgataaag ttgtttgttt tcagtggata
    17101 cttgaagttt ggcttccaaa gtatcgcatt gagattgaag cgtagctaaa taactgttgt
    17161 aagagagtgt gacctgccaa atgaattgaa agaagatatt gagattaggc gaaatatcac
    17221 tttttggaaa ggtctggcta aagactttct ttaaaaacag tggttcatga ttcgtaattg
    17281 tgataagctt ctttttgggc gtcaaaataa tagagagcgg gtatgtatcc acttgaaaat
    17341 aaccgctagg acttgtttta gcaaaaggat attgtaacaa taataacgct ggctttgtca
    17401 actttgattg attgaattct tctgtacggg aattttctgc atcgtccaat atggaagtga
    17461 tgtaatctga cggaatttca aattgatgtg tcagctgatg aatctcttct tgattaggac
    17521 gttctacaca ataccagatc gtttggtcag tttcctttcc ggtattacgc agtttctcat
    17581 tatctaatgt aaaatatttg atcatgattt tcctcctgtt gcctgtttct tttattataa
    17641 cggataggga attggaatac atggaatgtg tttgtaagat atagaatata actgcataaa
    17701 tttaaataaa atgaaacagt tgtgtaaagt atatacagta tacttgaagc aacgataaaa
    17761 aaggagttta cgttcatggg cacattcaca ccacttatct tgttttggta tatttacccg
    17821 gtaatcgttt tgtttggttg ccaatttctt gtaaaattat tttcgttaaa tcgtcgtttt
    17881 aaaataaaag cccctgattt ggcgattcct tttttgtggg gcggtatgca tgcattgtct
    17941 agaaatacag ggacaatttc tatcttaccg tttttcttta tttctgtttt gctaatggga
    18001 atttgtattg ctgttttcca agcatattat tatgaagaaa ttatttatcc gcgttatttc
    18061 aagatgactt ggcgtttggt ttttctgttg acaatcgtct tgtacgctgt cttgatcatt
    18121 gtgaacatac tttcttacct atgaaaaaaa tagaaaagct gtaaaaagtc ttttaaaagg
    18181 ctttttgcag cttgattttt aaaaaaatgt ttatgaataa aaaaagttgt tcccggattt
    18241 cccagaaaag gtggtagaaa gtgggggatt gtggtagact aattttagtt agtggaggaa
    18301 tggggggctt tcaatgttga tgggtgaatt ccaacataat attgatgcaa aaggacgttt
    18361 aatcgtgccc tcaaaacttc gtgaagaact tggcgaaaag tttgttctga cacgcggatt
    18421 ggatggctgt cttttcggct atccgatgag cgaatgggaa aatctggaag ctaaactgaa
    18481 cgaaatgccg ttggctaaaa aagacgcacg tacttttgtg cgattcttct attcggctgc
    18541 cactgaatgc gaacttgata aacaaggacg aatcaatata cccagcacgt taagaaatta
    18601 tgcagctttg acaaaaggtt gcgtagtcat cggtgtatct aaccgaattg aaatttggga
    18661 tgagacacga tggcaagatt tttctgcagc agcagaagag aactttgacg aaattgctga
    18721 aagtatgatt gatttcggac tataagaata ggaagaggct gactaatgac tgaaacattt
    18781 caacactata cagtcatgtt gaaagaaacg gtagatggat tgaacatcaa accggatggc
    18841 gtttatgtag actgtacact cggcggtgcc ggacatagtg aatatttatt aagccaacta
    18901 agttctaaag ggcatctgta tgcgtttgac caagatcaaa aagcaatcga tcacgccaag
    18961 ctgcgtttag cttcttatgt cgaacaaggt atggtcacat ttatcaaagc gaacttcaga
    19021 gaattagaag aacgcttgag tgagcatgta gataaggtag acggcatttt gtatgatcta
    19081 ggggtttcct ctcctcagct agatgaagca gaacgtggat ttagttatca tcaagatgca
    19141 ccgttggata tgcgcatgga tcagtcagct cctttatccg cttatcacgt aattaatgaa
    19201 tattcctacc atgaattagt aaagattttc ttccgctatg gcgaggagaa attttccaaa
    19261 caaattgctc gtgaaatcga acgagtacga aaaacaaaac caatccaaac aactggtgag
    19321 cttgtcgaaa tcattaaaca agtcatccca gctcccgcaa gaagaaaagg cgggcatcct
    19381 gcaaaaagaa tttttcaagc tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag
    19441 gcctctttag aacaggcaat ccggttattg aaagtcaatg gaagaatcag cgtaatcaca
    19501 ttccattcat tggaagacag aattgtcaaa agtatgttta aagaatatag tacggtacaa
    19561 gatctaccac cgggacttcc tgtcatacct gacgaattcc aaccagaact gaaagtcatt
    19621 actcgtaaac caatccttcc ttcagaggaa gagctaacag aaaacaatcg atcacgcagt
    19681 gcaaagttac gtattgcaga aaaaataaga atgaaagagg agtagaatga tggcagaatt
    19741 aaagaaaatg caagattacg aatatgattt acctgaattg gtggatcagc cggaacaaca
    19801 aatgcctgat tcttcttcga atcaagaaat cattcttcca caatcaccgg caaaacgatt
    19861 aaagcagatt tctgctttgg aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc
    19921 tttatgtgtt ttaacagtta tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac
    19981 gtcaatacaa acagaaatca atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa
    20041 aaacgaactt tctcgtactg aacggattaa acaaatcgca gaagaaaaag gattgtctat
    20101 taatgacgac aatttaagga aagtgaagta aatgagtctt aaaaataaaa ttcggcggtt
    20161 tatggaaaag aaaaatctaa atccgatgaa caatcgcaaa aaagtaggta tcattttatt
    20221 tgctacgagt attggattgt tctttttatt tgccgtccgt ttctcgtata tcgttattgg
    20281 aggtcatgtt gctggtacct cattagctga aaaaacaaaa cagttgtacc aaggaagtga
    20341 agtagtaaaa gcaaaaagag gaacgattta tgatcgtaac ggtgtggcgc tcgcagaaga
    20401 tgcctcctct tattcaatta aagcgatact ctcaaaaaca tatacctctg gggataaaaa
    20461 actgtatgtt gaagaaaaga attttgataa aatagcagaa atcctgcata agaatctttc
    20521 tatagacaaa aaagatgcac tgaatatttt agaggacggc gcaaaaaaag agctgtatca
    20581 agttgaattt ggtagctatg ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc
    20641 ggacatgaaa aaagaaggtg tcgctggtct ttattttgtc gaccatcagg ctcggatgta
    20701 tccaaatggc gtcttttctt ctcattttat tggatacgca gttccagaca aagatgagaa
    20761 tggcttagtt ggtaaactag gtttggagtc tgcttacaat gatatcttga gcggtaaaga
    20821 tggtaagatt atttatcaaa aagataattt tcaaaatcct ctgccaggta cagtggcaga
    20881 agaagaaaaa gctgtcgacg gacaagatat ctacacgact ttagacagca gactacaaag
    20941 ttatttagaa acactgatgg atcaagtaaa tgaagaatat cagccagaag aattgacagc
    21001 agtcctgatg aaagcaaaaa ccggagaaat cttggcaatg ggacaacgac ctactttcaa
    21061 tcctgaaaca atggaaggat tgactggaaa agatgctatc tggcgtaatt tcttggtaca
    21121 agatagttat gaaccaggat caaccatgaa agttttcact actgcagctg ctatcgaaga
    21181 aggagaattc aacgagaatg aaaccttcca atctggaaaa atccaagtag aagatgctac
    21241 gatcaatgac catgactttg gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg
    21301 gtcaagtaac gtcgggatgg tcatactgga acagcgatta ggcggacgat ggtataacta
    21361 tttacaaaaa ctgggatttg gacaaagtac tcattctgga ttagatgatg aagtaaacgg
    21421 tgctttacct acattaaata ttgtcgaccg agcaatgagt gcttatggac aagcggtagg
    21481 tgtgacgaat ttccaaatga tgaaagcttt cacttctatt gcgaataacg gtacaatgat
    21541 tcaacctcga tatatcagca aagtggtgga tccccaaact ggagaagagc gaacaactca
    21601 aacagaagtt ctagggcaac ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat
    21661 gagagatgtt gtcgaaagcg aaaactacgg tagtgcttat ggtgtatata gtgtacctgg
    21721 atacaatgta tcggcaaaaa acggaacagc ccaaatcgct tctgatacag ggggatatca
    21781 aacaggagat acagcctacc tgtattcgat tgtggagatg gttccttctg aagatccaga
    21841 ctatgtcttg tatctaacaa tgaaacaccc taagacatat gatcgaatgg cattggctaa
    21901 aatcgcaaat ccattgatga aacgggcaat ggattttaaa gaaactgaag aagattcaga
    21961 tacagaaaca aaaacagaaa aagtatccgt agccgattat cgtaatcttg aagcggacgt
    22021 tgcagcagct gatgctcaaa aaagtggact gcaacctgta gtgatcggta atggtaaaaa
    22081 agtacaaaaa caatctactg ccaacggtga tcagttgatc tctggagaaa aactgatcct
    22141 atatactggt ggtgataagc tgatgcctga cgtaacagga tggtcaaaag cagatatcat
    22201 gaagctgggg aaaatcctcg gtattgaagt tagttttgat ggagatggtt attgtgtcaa
    22261 acaagaactt gctccatatg aaaaaatcac tgaagacaaa cttagtttta ctttagaaga
    22321 ataagaagag aaataggaga gaaaagagca tggaatggac acaagcattg attcccattg
    22381 tcagcagttg tgcaatgaca attgccgcaa tgccgctatt tattggttat tttcaaatga
    22441 aaaagcaagg tcaggcgatt cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa
    22501 ctcctacaat gggcggctta gtgtttctga tcggctctat tttgacagga atttgggttg
    22561 gagcttggca aaaacagcta actcctactc ttttcatctt gctttttgta ttagcgttat
    22621 acggagtaat cggctttctt gatgatttta taaaaatatt caagaaaaga aacatgggac
    22681 tgaactcgaa gcagaaattg attggacaga ttctaggggg aatcatcttt tatcttgttt
    22741 accgttctga aggttatcca ggtgtattga atttcttcgg actagatcta cctttaggaa
    22801 tcatttatgg tattttcgct ctcttctggc ttgttggatt ttcaaatgca gtaaatctga
    22861 cagatggcat cgatggatta gttgctggct taggaagtat ctcttttgca gcttacggtc
    22921 taattgcttg gcatcagcat cagtatgatg tattaatcat ttgcttaagt gttttaggag
    22981 gtttactggg tttcttcgtc tacaacagaa aacctgccaa gatttttatg ggagatgtcg
    23041 gttcattagc gttgggcgga cttttagcgg caatttctat aatgctaaac caagaatgga
    23101 cattgctttt gattggtttg atctatgtta tggaaacagc aagtgtgatg cttcaagtta
    23161 cttcctttaa gcttacagga aaacgaattt ttaaaatgtc tccaattcat catcactttg
    23221 aaatgtgcgg atggtcggag tggaagattg atacagtttt ttggttgatc agcattgtga
    23281 cttcgttgat cacattatgg atcgtttggt aggagatgac gggaaatgaa aaaaatatca
    23341 acatatgaaa ataagaaagt gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc
    23401 aaattattac atgaattggg cgcacttgtc accgttaatg atggaaaacc ttttgacgaa
    23461 aatccggaag cacaagagct gctttcttta gggataaaag taataacagg tagtcatcca
    23521 atcgaattgt tggatgaaga attttcattg atggtcaaaa atccagggat tccctacagt
    23581 catcctttag tagcaaaagc ccaagaaatg ggtattccgg taattacgga agttgagcta
    23641 gcttacgaag tagcggaatg cccaattatt gggattacag gaacgaacgg aaaaacaacg
    23701 acaacaacga tgacaggact actactgaat gcaggagcag atcaaggaat tgcacgttta
    23761 gcgggtaata ttgggtatcc agcaagtggt gtagcgcaag aagcaaaatc agaagataaa
    23821 attgtgatgg aattgtctag cttccaactc atgggaatca cagatttccg ccctcatata
    23881 gcggtgatta ccaatatcta tgaagcacat attgattatc atggtacaag aaaagaatat
    23941 gtcaaagcta aatggaacct tcaaaaaaac atgacagaaa aagattatct gatccttaac
    24001 tggaatcagt cagaactaca agaattagcg caaagaacaa aagcacgcgt actgcctttc
    24061 tcgacaaaag aagtgttgga agacggtgtg tatgctgacg attactcaat atactataaa
    24121 aaagaaaaaa taatggaaat atctgaactt ggcgtaccag gtaagcataa tgtagaaaat
    24181 gcactagctg ctatttctgt agcaaaattg tatggtatat caaatgaagc tatacgagaa
    24241 acgttgcatt ttttccatgg cgtaccacat cgtacgcaat atgtaggaga aatccaaggc
    24301 agaaaatttt ataatgattc aaaagcaacc aatattttag cgactaaaat ggctctaagt
    24361 ggatttgaaa cgtctaaggt cgtgttattg gctggcggat tagacagagg aaatacattt
    24421 gatgaattaa ttccttcatt aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa
    24481 gaaaagttga tggatgctgg aaaaaaagca ggcatcgaga caatagtaac agccgattct
    24541 gtagaacaag cagttccttt agcattagaa aacagtacag atggtgatgt agttttatta
    24601 tcaccagcga atgccagctg ggatcaatac ccaaattttg aaactagagg aaacagattc
    24661 atggaagctg tgaaccgatt aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat
    24721 tttggttaca ggcggaggaa cagggggaca tatttatcca gctcttgcct ttgtcaatta
    24781 tgtaaagact caagaaccga atgctgaatt catgtatgtg ggagcaaaac gaggtctaga
    24841 aaacaaaatc gtccctgata caggaatgcc tttccatacc ttggagattc aaggatttaa
    24901 acgaaaaatt tccatgcata acatcaagac ggtccgattg tttcttaaga gtatccgtca
    24961 agcaaaaaaa atcctcagtg attttcaacc agatatcgtt atcggtactg gaggatatgt
    25021 ttccggagcg gtagtgtacg ctgcagccaa aatgggtatt ccaacagtta tccatgaaca
    25081 aaacagtgta ccaggtgtta ccaataaatt tttaagccga tatgtggatc ggatcgctct
    25141 ttcatttaaa gatgcggctt ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc
    25201 tcgagcacaa gaagtagcag atactaaaaa atcagatatt ttgaaaacat ttgatttgga
    25261 tccagaaaag aaaacagtct tgatttttgg cggtagtcaa ggagcactta aaatcaatca
    25321 agcagtatca gtttttttaa gtgaatatat tccttcttct taccaagttt tgtatgcttc
    25381 aggagataga tattatgaag agatcctaaa taaaataaat cctgaaatac aaaatattag
    25441 tgtccagcct tatatcaaaa atatggcaga ggtcatggct tccagtgatc tgctggtggg
    25501 gcgtgctggt gcaacttcga tcgcagaatt cacagctctg gggttaccgg cagtgttggt
    25561 tcccagccca tatgttacga atgatcatca gactaaaaat gcaatgagtc ttgttcacgc
    25621 aggagcagca aaaatgattg cagataatga actaacaggt gaatcactaa gtcagacagt
    25681 caacgaaatc atgggtgatg aagaattgca aaagcaaatg tgccgagctt ctaaagaaca
    25741 aggaatccct gatgcttcaa aacgtcttta tgacttagta aaacaaataa tcaaaaaata
    25801 aaggcggtgg tcttagtgag taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct
    25861 gatgaaaaca atttgactcc ttggcaaaag gctaaccgaa aatatctggc agaacaagag
    25921 atgaaaaacc aagagccaaa aaaagaagac ataacattag atacacccct ttcagattct
    25981 gaagaagaaa aaacggttca agaggaacag caagaagaaa gccagtccga gaaaaaaatc
    26041 gaattttttg aagagatcaa gcaaaagaaa aacggtggac catataacgg ttcttttttg
    26101 aaccggctgc ccaatttaaa aagtcaacgg cacaaagtac tatttcgtcg gttagctttg
    26161 atcatcactg tattgggtat accgctgatt tttttgatct attatgtttc tccttatagc
    26221 aaattacaag cagttgcagt atcaggcaat aagacagtca acagccaaga aatcatctct
    26281 gatacaaaat tgtcattagg agaaaatgtt tggggacagt attttcatcg atctacttat
    26341 atcgatcgcc ttaaaaaagc acagccaaga atcgaaacag caaatataca tttcaaaggg
    26401 atgaatgaat tcgatttgga tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac
    26461 aatcaatact acccagtgat tgaaaatgga acagtattag atgaaaaagt agcaaatcct
    26521 acaaaaaatc tccctatctt ggaagagttc aaagactctg caaaaatcaa agaattaaca
    26581 aagcaataca atcagttatc atcagaatta caaaaagcaa tttctgaaat caagtacacg
    26641 ccaagagcaa gcaataaaaa tctgatccaa ttgaatatga atgacggcaa tcaagttatt
    26701 gtaaatatca ataatcttgc gaaccaaatg aaatattatt cgcaagtagc caaagatatg
    26761 gatgaaaaag gtgtgatcga catggaagtt ggtatcttct cgcatcctta cggtagcact
    26821 caagaaacgg aagaatcggg ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat
    26881 caaacaaacg aatcaagtga agaggcagaa acgcaaaaag cacaaacacc tggatcgtct
    26941 agcacaaacg gatcatctga ttcaaatgat tcaagtgaaa caagtcaaat agaagataat
    27001 ccaagtacaa ctccgtcaag tttaaatgaa aattgataaa acaactttaa aaagtaagaa
    27061 tgtactttct aaaaaccagt aatttatggt agaattggaa gcagtgaata tatctattaa
    27121 taagtatatt ttaaaacgga cgctagttgt tcgcttgtat atggaatagt aagtaggagg
    27181 gaatcccatt catggcaaaa acaggaatgt atgtaggcct tgatattgga acgacatctg
    27241 ttaaagttgt cgtggctgaa tatatcgaca gccaaatgaa tattattgga gtaggaaacg
    27301 caaaatcaga agggattaac cgaggcatta tcgttgatat cgataaaacg gtccaagcga
    27361 tacaacgagc agtacgacaa gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg
    27421 tcggtttgcc agcaaacatg cttgaagtag aaaattgcca gggaatgatt gcagttaacg
    27481 gagattcaaa ggaaatcacc gatgaagatg tcagaaatgt ggcttcagct gcccttgttc
    27541 gttcaatacc tcctgaacgt caaatcgtgt caatattgcc acaagatttt actgtcgatg
    27601 ggttcgaggg aatcaaagat cctcgtggaa tgatcggcgt aagattagaa atgtacggtc
    27661 tgttgtttac aggacctaaa acgatcatcc ataacatacg taaatgcgtg gaaaatgccg
    27721 gattaatcgt caacgaaatg gtgattactc ctttagcgct gactgaatca attctttctg
    27781 acggagaaaa agattttggt acgatcgtca ttgatatggg tggcggccaa acaacaacgg
    27841 ctgttatgca tgataagcaa ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg
    27901 tcactaaaga tatctctatt gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa
    27961 taaactacgg ggatgcatac ccagaacgga catcaccaga tgaagaattt ccagtagatg
    28021 tgattggtca gtcagaacca gtgaaagtcg atgaacgcta cttgtcagaa attatttcag
    28081 ctcgaatgga acagattttc aacaaagcaa aagatgcatt ggaccaaatc gaagcattag
    28141 agttgccagg tggcgtcgta ttgacaggcg gagctgctag tcttcctggt gttgtggact
    28201 tggcacaaga aatcttcggc gtaaatgtaa aattgtatgt tccaaatcac atggggctga
    28261 gaaacccggt atttacaaat gtaataagta tcgtggatta ttcagcaaac ttgagtgaag
    28321 tgtatcaatt ggcaaaaatt gctgttacgg gtgaaacctc agcagctcgt caaatggtag
    28381 ttgaacaaga aacaacaaac acatatgaga gctatgaagc acctgaagaa acaatctacg
    28441 atgagccgga accaaaagag tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta
    28501 caaatatttt tgattaattc atcctgggag gaataagata tggaattctc aattgataat
    28561 aatatcaatg acggcgcagt aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct
    28621 gtaaaccgaa tgattgaaga aaacgttaaa ggtgttgaat tcattacagc caacacagac
    28681 gtgcaagcat taaaaaattc aaaagctgaa acagttatcc aattaggccc caaatatact
    28741 cgtggtttag gtgctggttc tcagccagaa gttggacaaa aagcggcaga agaaagtgaa
    28801 caatccttac gcgaagcttt agacggtgca gatatgattt tcatcactgc aggaatgggt
    28861 ggaggaaccg gtacaggtgc tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca
    28921 ttgactgttg gtgtcgtgac acgtccattc acttttgaag gaccgaaacg tggccgtttt
    28981 gctgcagaag gtatcgctcg tttgaaagaa aatgtggata cattattaat tatctcaaat
    29041 aaccgtttat tagaagttgt agataaaaag acaccgatgc ttgaagcttt ccgtgaagca
    29101 gataatgttt tacgacaagg tgtacaaggt atctctgatt tgatcacagc gccaggttac
    29161 gttaaccttg actttgctga cgtgaaaaca gtgatggaaa accaaggtac agcattgatg
    29221 gggatcggtg tggctagtgg agaagatcgc gtagtagaag caacgaaaaa agcaatctcc
    29281 tctccattgc ttgaaacatc tattgatgga gctgaacaag tacttcttaa catcacaggc
    29341 ggtttggata tgacattgtt cgaagcacaa gatgcttcag atatcgtagc aaatgcagca
    29401 actggcgatg taaatatcat tctaggtaca tctatcaatg aagaaatggg cgatgaaatc
    29461 cgtgtgacgg tcatcgcaac aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga
    29521 ccagctagac aagcacaaat gcaatcacct gctcaaaaga ctgtattaga tatggatcaa
    29581 gctaaaccaa cttcttcaga agaagaaaac agttttggcg attgggatat cagacgtgaa
    29641 caaaatgttc gtcctagagt tgatgattcc aattttgaca atatcgaaaa aaaagaattt
    29701 gatacattca accgcgaaga aacaaaatct catggcgatg atgaattaaa tacgccaccg
    29761 tttttccgtc gtaaaaaata aaggagagga agagcacatg attgctgaca acttgcagaa
    29821 aatcaagcaa gaaattcagg attcgtgtgc tcttgttcat cgtgatccga aagaagttac
    29881 attagtagct gtaacaaaat ctgttgaatc cgctcaagct gaagaattgg ttagagctgg
    29941 cgttatgaat tgtgcagaaa accgagtaga taaattatta gaaaagaaac aagatttatt
    30001 tgattttcct gaaataaagt ggcatttaat tggtaattta cagcgcagaa aggtaaaatt
    30061 gattgtaaat gaaattgatt actttcatgc gttggacagt ttaagattag cagaagaaat
    30121 ccagaagcgg ttagagaaag aattatcctg ttttattgaa gtaaatgttt ctggtgagga
    30181 atcaaaacat ggaatttcac cagatgagct tttaccgttt gtggaatctt tagctgcctg
    30241 cacaaaaatc aaggttgttg ggttgatgac tatggcgcct aaagaagctt ctgatgagca
    30301 aatccataaa gtgtttggta cgctgaaaga attgcgtgat cttgttcaag caaaaggtta
    30361 tgcccatgca ccatgtagtg aattaagtat ggggatgagt caagactttc aaattgcgat
    30421 tgaagaaggt gcaacattta tccgtgtagg aacagcgcta ttcaaagacg aataaaggag
    30481 gaacaaaaat gccgctttta aacagaggag caattgctaa ttttttcgga ttagctgatg
    30541 aggaagagta cgaataccaa gattatcctg aaacaacaaa aaaggctgta cagccaacta
    30601 aaaaaaatac aacacagaat caatcaactc attctgtatc tgctgcgaat actcatgaaa
    30661 aacaaaaaac ggcgacttca cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga
    30721 aaaaagtaat tgaactacat caatcaccta caaaaactgt agcgaaagaa agaacagctt
    30781 caagaacctc tacacaacct aaagcaaatc ttgctggaaa aatcactgta atggaaccaa
    30841 gagcttattc tgaagctatg gcgattgcta aacgaatcat tgctggagaa gcaaccttag
    30901 tgaattttca tttagtagaa gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa
    30961 cagtttatgc actagatgga gatatcaaac gtgttggaga tgagattttt ctttgtacac
    31021 cgactgggat ggagattgac agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg
    31081 atctgtaaga ggaggaacta cgatttatac tattgcacta ttacttcatt tacttcaacg
    31141 ggcagcctat ttctatagca ttatcttgat ggtatatgca ttgctctctt ggtttccagg
    31201 cgggtatcaa tcagggctag gacgattatt agctaagatc tgcgaaccgt atatcagttt
    31261 gttcgatcgt ctgcctttga ggattggacc cttggatttc acaattttag gggcaatcct
    31321 gatcttgaac ttagccgtac aaggtttagc acagatcgtt atatggataa ctgtccattt
    31381 agtttattga ttgaagcaac aaaatcttta ttctcagaat agataggagg ggaaaatttt
    31441 ggatgcaaat gtataccagc actttcgggc agatgaacgt acatttatcg attcggtagg
    31501 cgattggatt gaacaggttc aaggacaata tgcaccctat ctgaccgaat ttcttgaccc
    31561 aagacaatcc tatattttag aaacactgat tcgacaaagc agtgatttgc gtttcatgtt
    31621 ttatggcgga tatgaacagg cagaaagaaa aagatgtttg attttccccg attattacga
    31681 acctgtagag gaagattttg aaacagcatt gtatgaaatc cattatccta gcaaatttgc
    31741 cagcctaagt catgggaaaa tccttggaac tttagtggga acggggatca aacgctctta
    31801 ctttggcgat atcatctcgg acggtgatcg ctggcaagta tttattgcaa atgaggtgga
    31861 tcattttgtt gctgcacaag taacaaagat tggaaaggtt gctgtacgct tggaaccaat
    31921 caaatacaca gaaatgatta tgccgaaaga tagctggaca caagaaagag gaacagttag
    31981 ttccatgcga ctagacagtg tgatctctga gatttataat atttccagac agcggtccaa
    32041 gcagctgatc gaagctggta aagtaaaagt gaactggaca gaaaatacaa gacctgattt
    32101 tgtacttgaa ctattagata ttatttcagt cagaggttat ggcagagtcc agatccaaga
    32161 acttgagggg aagacgaaaa aagaaaagta cagggtactg ttcggtgtat tacgtaaata
    32221 aaataaaaag gtggtttact catggcttta actccattag atatccaaaa taagacattc
    32281 ccgacaaaaa tgcggggata taatcaagac gaagtagatg atttcttaga tttagttgtt
    32341 cgtgactatg aggaattgac tcaaagaaat cgagaattag aaaaagcagt caaacattct
    32401 gaagaaaaac ttgagtactt caatgaatta aaagatgctt tgaaccaatc aatcatcgta
    32461 gctcaagata cggctgataa agtaaaaaca agtgcaagca aggaatccga agtgatcgtt
    32521 acttctgcac aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa
    32581 ttaacaactg atgcagaaga aaaagcaaga aaaatcttga cagatgcaac agaaaaagca
    32641 agacagcttg cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt
    32701 atcagcttga tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt
    32761 ttacaaccat tctccagcta cgtgactgac agccatgaag tgatcaaaga agttttagca
    32821 aaacagcttg acaatgaaaa tgatactgat gtaaactcag acacaggagt catagaagca
    32881 cctatgacgg catttgatac acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa
    32941 aacgagtaga acagaaaagc actttgttat tcttaaagcg agccgatgag agtggaagtt
    33001 cggtaagaca ctgcatggtg aaagatcctc tacttgagac ctgcttttcc gaaattagag
    33061 taagaagcag cggctgacct cgttattggt cacagagagt ttgcactaga tagatgcaac
    33121 taaatttggg tggtaccacg gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc
    33181 cggaaaacaa ataaaggagt gtatggttga atgaaaatga aagaaacctt gcaattagga
    33241 aaaacaaaat tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa
    33301 tgggaagaaa atcgaatcta ccaaaaaaga caagagttga acgaaggaaa acctagcttt
    33361 gttcttcatg atggacctcc atatgccaat ggtaatatcc atttaggaca ttcattgaat
    33421 aaaatcagta aagacattat cattcgttca aaatcaatgt ctggctatcg tgcaccatat
    33481 gttccaggat gggatacaca tggattgccg attgaacaag tattggctaa taaaggcgtg
    33541 aaacgtaaag aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc
    33601 caagtagata agcaacgagc agatttcaaa cgcctaggag ttgcaggaga ttgggaacat
    33661 ccttatgtta cattagatcc ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg
    33721 gctgaaaaag gctatattta caaaggattg aaaccaattt actggtctcc gtcaagtgag
    33781 tcttctttag cggaagctga aattgaatat aaagatgtaa aatctccatc tatttttgta
    33841 gctttcaatg tcaaagacgg caaagatatt cttgatgaag atacagcttt tgtcatttgg
    33901 acgacaacac catggacatt gccagcaaac caaggtattg ctgtaaatcc aaaatttacg
    33961 tatgtattgg tggaagccga tggccgtaag tttgtcgtag cgaaagattt gctagaaaca
    34021 gtccaatcag aaatcggttg ggaaaatgta acagttctaa aagaatttgc tggacaagaa
    34081 atggaatata tgactgcaag tcatccattc tatgaacgtg aatcacttgt gattttaggc
    34141 gatcatgtta ctttagacgc tggtactgga ttagtccata ctgctccagg acatggggaa
    34201 gatgactata tcgctggtag caaatataaa ctatctgtag tgtcacctgt agactcaaaa
    34261 ggattgttta cagaagaagc acctggattt gaaggtatct tttatgacaa agcaaatcct
    34321 atgatcactg atctattaaa agaaaaagga gctttgctga agctagattt ctttactcat
    34381 agttatcctc acgattggcg gacgaaaaag cctgtgatct atcgggcaac tccacaatgg
    34441 tttgcatcta tcgataaatt ccgtcagaat attttggatg aagtagaaaa agtagagtgg
    34501 gttatcccat ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg cgattgggta
    34561 atttcccgtc agcgtgcatg gggagtacca cttccgattt tctatgctga aaatggagaa
    34621 gcaatcatca caccagaaac aatcgaacat gtggcaaaat tatttgctga acatggatca
    34681 attatctggt ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac ccacccaggt
    34741 tctcctaatg gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt tgattctggt
    34801 tcttctcatg aagctgtttt acgtcagcga ccagaattat cattcccagc agatatgtat
    34861 ttagaaggat cagaccaata tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct
    34921 atcaatggtg tagcaccata caaatcagtt atttctcaag gatttgcttt agatggtgaa
    34981 ggccgtaaga tgagtaaatc tttaggaaac atcattgcac cagaaaaagt gatcaaacaa
    35041 tttggtgcag atatccttcg cttatgggta tctagtgtag attatgaagc agatgttcgt
    35101 gtttctatgg atatcttaag tcaagtggca gaagtatatc gtaaaatcag aaatacgatg
    35161 cgtttcttat tagcgaatac agaagatttc gatcctaaaa aagatactgt ttcgtacaat
    35221 gatttacgtt ctgtagacaa atatatgacc gttcgattga accaagtaat caaagaaatc
    35281 cgagaagaag ggtacgacaa atataacttc atgcatattt accgtacagt tatgaacttc
    35341 ttgacggttg atctatcgtc attctacctg gattttgcta aagatgtagt ttatattgaa
    35401 gcagaagatg attatcaacg tcgttgcatg caaactgttt tctatcaaac agctgtagct
    35461 ttgacaaaat tattgactcc tatcattccg catacagcag aagaaatctg gtcattcctg
    35521 aaagaagaag aggagtacgt tcagttaagc gagtttcctg gttatgaaga gtttgcaaac
    35581 caagaagaat tgatggatac ctggactgct ttcatggact tccgtgataa tgtcttaaaa
    35641 gcattggaag aagcaagaaa cagcaaattg attggtaaat ctttggaagc aaaactaact
    35701 gtttatccaa aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa
    35761 ttattgatcg tttcaccaga ctactttgaa gttatggcag cagatgaaca agtgccagat
    35821 aacgctatgg tatttgatga tgttgctatt acagtggaaa aagcagacgg ggaaacttgc
    35881 gatcgctgtc gccaagttcg taaagatgta ggagtagatg aaaaacttcc tcatctatgc
    35941 ggtcgttgtg caaaaatcgt tgaagacttc tatcctgaag ctgtagctga aggattcgaa
    36001 gaaaagtaaa agggaaaagg gggtgtgaca tgttctttgt cacaccccct tttgttatca
    36061 atcgtttaat tttccacgtt cacgatacca gttatctggt tgccagatcc attcgaatcc
    36121 gtctttttca agtagttcga aagctgcttt aggtcccatt gatccagctt tgtagttggg
    36181 aaaatccggt tgtgtttggt cccaagcttt tcgaatgaca tccacgatat gccaagattg
    36241 agcgacttct tcccaatgag agaagttcgt tccatcacca ttcaacgcat ctaatagtaa
    36301 tttttcgtaa gcttctgggc tattttcaac catttctgca ctatttctaa attccaattt
    36361 gattggttcc gtttcaaatc cttgccctac ttctttccca ttcaaggata aagaaaatcc
    36421 ttcagttggc tgaatataga tcgttagaac attaggtgga agtgtcgtat cgtcacatgg
    36481 ttcatcgaca gatgttttga atacattaac aggaacttgt ttgaatacga tgttgatccg
    36541 agtccctttt tcagtcaaac gtttgcctgt acgtacatag aaaggaacac ctgaccaacg
    36601 gaagttatcg atagtgaatt tgcctgcgac gaatgtttca gtctctgatt gagcatccac
    36661 tttgtcttct tcgcgatagc ccttgaattg tacaccatcc aattcacctt gagcatattg
    36721 cccgcgaacg aagttttcta acacttcttc ttcgttataa atgcggatag ctttcaatgc
    36781 tttgatcttt tctgtacgga tttctttttc ggaaaatgct actggcggtt ccattgcaag
    36841 taatgctaca acctgtaaaa tatgattttg caccatgtct ttaagtgcgc cactatgatc
    36901 gtagtagccc ccgcgatctt ctacaccgag gttttcgcca aaagtaattt ggatattgtc
    36961 gatatagcga ttgttccatt gagcctcaaa aatattattg gcaaaacgga tagctgagat
    37021 gttttggatc atttcttttc ctaaataatg atcaatcctg aaaatatctt gttctgggaa
    37081 tacttctctg atttgctggt tcaattcaaa agcagactgg taatcagaac cgaacggttt
    37141 ttctatgatc aaacgattga agccattttt tgtcataata ttttgtgatt tcaaatgaga
    37201 aacgatcgtt ccaaaaaatt gaggtgccat agccaaataa tagacacggt taccgtttaa
    37261 ttgatagtgt ccatctaatc ggtcagacaa ttcttttaat gtctgataat gcgttgaatc
    37321 atttacatca tgggattgat agtaaaaatg gcttgcaaag gtagttgctt cttcatctga
    37381 aggattcaat gcacggattg tttctttgac aacttcacgg tagtgctcat cgctccaggg
    37441 acggcgagcg gttccgatta cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg
    37501 gaacaaagac gggtacaatt tacgctgggc taggtcacct gtaccgccga atatcgtaaa
    37561 caatacattt ttttcattag acatgtggat acactccttt atattttatc gacaaaaatc
    37621 gtgctggccg ccttaaagct gacggggatt ttctttctat cattttggat agtgatcggt
    37681 ccttcataag ctgcgatgtc aacgatctca tattcttcgt tgatattcag gtcaatagtg
    37741 actaagtaat ccaacaattc tttttcatca agcactcgtg cgatacgcac ttttgtaccg
    37801 acaggatagg actccaatgt ttgtcggcgt tcttcatgga caacttcctc ttttttagga
    37861 atcgatcctc catgaggaca atgttcagga tgattcaaat agttttctaa atgatctgca
    37921 agatgttccg aagtcacatg ttccaatact tcagcatcat catggacttc attccagcta
    37981 tagtctaaat gttcaactaa aaaaacttcc cataagcgat gtttgcgaat cagactgcta
    38041 gctttttgca gtccgcttgc agtcaattgt acaccttgat aaggggaatg ttctacaagc
    38101 tcttctttca caagtttaga aatcatttca ctgacagatg cagctgagac atctagacta
    38161 gatacgattt gtttattgtt gactttcgtt gtatctccac ctagttctaa aatgattttc
    38221 aaataatctt ctcgatttgg tgtcatattc gatcatcctt tcatggtaaa aaaccatcat
    38281 tagtaagtgt aacaaaaaaa acgttaattg actatttttc taaataaaaa acaaaggatt
    38341 tttagacgtt tttacaacaa aaaacgaaag atgatagaga agactcattc tttcgtttat
    38401 aaaggatata tatatacatt gggagggatc tatttttcag ataacaactt atatgaagtg
    38461 tctgcagatt cgtcataaag agtttgttcg ggataagaaa agataagata aaaaccatac
    38521 tgatcacgta cgactaaacg atattcatct atttctacca cagatgtgcc aagagactga
    38581 ccgttgatca aaaacgtgta atcagatgta atctgtaatt gttgtttttg ctggctatca
    38641 ctaaagaacc atgtaccttg atactttgag agtccttctg acttttccgc ttgttttttc
    38701 tttttcaagt aaataaaact tgagagaacg gtaatgagtg aaccaagaaa atagatcatt
    38761 ttttttatca ccaaatcact cctttatgag aaaataatcg catagacata ttctttcttc
    38821 tttatttcat tgtaccgaat aaaagctaaa atttctctaa gactttcgac tggttttttt
    38881 actgtttttc tacaaattat gataggatac atagtaggaa gaaaggtgac ataatttgaa
    38941 gtatcagcaa gtggaaatag ctttttttat tgaccgcccg aatcggttta tagcacattg
    39001 tatgaaaaaa gacggagaaa taattattgc gcatgtcaaa aatacaggta gaggaaaaga
    39061 ggtcctgata ccaggcgctg aagtggctat tgtatttgct cctggaccta aacggaaaac
    39121 cgcctatgat ctgattgctg taaaaaagaa gggtgactgg tttaatatcg atagtcaatt
    39181 gcctaatcgg ctggcaattg atggtatttt agacggcacg atccttttac caggattaac
    39241 tggtccattt acggtataca agcgtgaagt aacttatctt cactccaaat ttgatattta
    39301 tttagagacg gtggtcggac aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa
    39361 taaagcgatt ggtgcttttc ctgatgcgcc cacactcaga ggattgaaac atataggaga
    39421 attaacgtat gccgcacaag atggttatgc cgcttatgtc ttgtttattg ctcagttcga
    39481 acatcttcat ctggcaacga tccatgaaga aatgcaacca gcactagctg atatggttcg
    39541 acatgcgcag caaagcggag ttcaaatcct cgcctataat tgtcaagtat cggcagatga
    39601 ggtaactgtc aagcaggcca ttccatttga tgtagatatc ccttttgaag atcctataca
    39661 atagaaagaa ggtaaaataa tgatcaactt aggtattatc ggaacaaatt ggattactca
    39721 tcaatttgtc caagcagctt taagcacaaa aaaatatgaa ttgacagcgg tatattctag
    39781 acatttggaa aaagcacagg aatttggttc tcattatgaa ggggatatcg aattcgcaac
    39841 agatttgaag acttttttca atattactca tatggacacc atttatattg cttctccgaa
    39901 tagtcttcac tttagtcaag ctaagcaagc aattttggca ggaaaaaatg tgatagttga
    39961 aaagccagct ttttcgacgc caaaagaaat ggatgaaatc atccatttgg caaatgaaca
    40021 caatgtgtat ttttttgaag cagcaagaaa tatccatgaa tcaggtttta aaaagattgc
    40081 cgattactta ccattaaaag acgagataat cggtgcaaat ttcagctata tgaaatattc
    40141 ttcgcgctat gaccaagtat tagatggaga agaaccgaat atcttctcga ctcatttctc
    40201 aggtggtgca ttgatggatt taggtgttta ccttgtatat gcagctgttg cttggtttgg
    40261 aatgccaaaa gaagtccatt atttcccaag aaagatttct actggtgtag atggtcttgg
    40321 atgggggatc ttacgttatg agaattttga tgtatcgatc cagccaggta aaatcggtga
    40381 ttcttattta ccgtcagaga tttattttga ctcaggaacg ctagtgatga atggagtcaa
    40441 tgcaattgaa caagcacaat ttcatgatcg cgcgcataaa gaaatcattg atttggcggt
    40501 aacagctgca gaaaatccaa tggcagatga agcagaggat tttgccaatg tcatggctca
    40561 tccgaaagat ccagcttggg gattgttgta tgaagagtgg atcgagctag ctagaaatgt
    40621 gaaccaagtg atttatgatt taagaaaaaa tggcggcatc aaattcgatg cagataatga
    40681 ggaagatttt taatgaacga atttgtacaa gtatttcccg aaaattggca gaaaaaatat
    40741 gaggataaag ggtttatctc accttctctt attcaacgag cagtttttca gccactaagt
    40801 aataaacaaa gtatagtagc ggtttctcct acaggttcag gtaaaacact ggcttatctt
    40861 tggcctctac tactaaacgt ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct
    40921 tctcaagaac tagctattca agtagcagat gttgcacgtg aatggggaaa agacaaagag
    40981 ttgaaagtcc aatctttggt gggcggagcc aacgtcaaac gtcagattga gggtttgaag
    41041 gaaaaaccag aaatcttgat tggtacacca ggaagaatat tggaattgat gaaagcaaaa
    41101 aaaataaaag cacatcaagt gaaaacaatg gtatttgatg aagcagacca gctatttgac
    41161 gctgggaaca gccaaatcat tgatcaaatt cttcatcaag ctccaacaga gtatcagcta
    41221 gctttttttt cagcgacagc agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa
    41281 acgtttgaga caattgacgt gacagcagag gacgattctc gaaaaggact gagacattac
    41341 tttttacgtg taccgatacg aaagaaagat gactacttga gacgtctcac gcatatagaa
    41401 gattttcatg gtctgatttt ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa
    41461 ttgctgtata gaggtgtacc ggtaggaagt ctggcctctg atcagaacaa gctactccga
    41521 aaagcagcgc tagaacaatt caaaaagaaa atggtcagag cacttttgac cacagatgtg
    41581 gcggctagag gtttagatat tacaggtctt ccttatgtag tgaatgcaga agttccgtta
    41641 agtgaagaag cttatttgca tcgttcggga agaactggtc gaatgggtaa tgaaggaata
    41701 gtaattacac tcgtacaaga taataatcta agagatttaa aacggctttt acgtccatca
    41761 aatataacac ttgaagagat ttttttatac ggaggtaagc tgcaaactga gcagccaacc
    41821 aaagaagatg cagctcctgc agtaaataaa aagtatgcga aaactttttc tggaaaggag
    41881 cagaacccag aatataacaa agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa
    41941 gagagaaata aaggagcaag gcgcaaataa gcgccttttt atgctatgat tatgaaaacg
    42001 ggaacaaaat tggttgccag aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc
    42061 agctattgac agaaaaccaa caaggattga cagcaagtga tgtagctgag cgcttaacta
    42121 tcgatcgaag caatgcaagc cgatatttaa gtgaactata caaggcccat catattgtaa
    42181 aaaccgctgg ccgaccagtc gtttactctt tgccaacgga aaagtcaaaa tctgatgaag
    42241 tgcatgtcga ctcttctacg caagtgactt ttgaaacact cgttggagaa aatgattctt
    42301 taaaagtcag catacagcag gcaaaagctg cgattcttta tccaccgcgg gggttgcata
    42361 cgattatttt tggagaaact ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt
    42421 ttgcaatcga ttcagagatg ctttctgcag atgcgccatt tgtttctttc aattgtgcgg
    42481 attatgatca aaatccgcaa ctgctctttg gacacatctt tgggatcaaa aaaggggctt
    42541 acacaggagc agctcaagat agtcccggtc taatagccaa agcagacggc ggtatattgt
    42601 ttttagatga gatccaccgt ttaccgccgg aagggcagga aatgctgttt actttcattg
    42661 ataaaggtat ttaccgtccg cttggagaaa gtgcacaagt acatgaagca tctgttcaga
    42721 tcattggggc gacgactgaa tcttcagaaa gtttcttatc tacgttcaat cgacggattc
    42781 ctatggcaat caccttgccg aatcttgctt cacgttcact ggatgagcgt tatgaaatca
    42841 tctctctttt tatcaaacaa gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag
    42901 aagcgattct tgccttcatg ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag
    42961 acttgaaact agtctgtgca aaatcttttt tacattaccg tactcatcat gagaaaaagc
    43021 taatcattcg aaaagaagaa ctttcccttc aagtacaaaa aggattactg aaaatcaagg
    43081 aaataccgga acgtctcgac cgattcatgg acagcaaaag tcagtatttg acatttgaac
    43141 caggatttgc tgatgttgtc tggtcacaag atccagaacg aaacatgcag gtctataacg
    43201 atattgagga aaaagtcact tctctttccg aaaccggtgt ggagaacatt gatctagaga
    43261 ccctcatttc aaaagatgtg gatgcttatt tccagacata tgtcaaagaa ttgactaaaa
    43321 gttcgattcc caaagatctt ttgccagatg aaatctggcg gttaaccaat caattgtatg
    43381 atgtggctga aaaacggtta gaccgtattt ataatgaaaa agctcgtttt gcttttgctt
    43441 tgcatctgca aagtacattg gatcgtgtga gagaaggtca tatgatcgtc cacccagatt
    43501 tgaataacgt acggaaaaat tttaagaggg aattccaggt agcaatcgat ctttcaacga
    43561 tcattgaaga agaagaacaa gttgagattc cttttgatga aatcggcttt attagtatgt
    43621 ttctttctat caatctagga gaaagtgagc ctgttaaaga aaacaaagta gatgttctca
    43681 tattgatgca cggcagacag actgcttcaa gcatgttgga gatggcacaa gaactattag
    43741 gaacaaaagt agggaaagct tttaatatgc cactggttat ggaagttcaa acaatgtatg
    43801 aagaagtgtt acaatatgtc aaaaactatc aagagcattt gacgaatggc ctactcttgt
    43861 taacagatat gggatcatta aacaccttca gtgatttgat ttatgaagag actggcattc
    43921 ggacgaaagc tatcacgatg acaagtacca tgattgtgat agaagcgata cggatggcaa
    43981 gtgttggaag aagcttagaa gatatttatc aaaatatcca gctttccttt gaatcgatcg
    44041 tacgtgatca gttcaaagcg gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg
    44101 tgacatgctt tactggtgaa ggagtagctg ccaaattata tcagcgtatt atgccagtga
    44161 ttaatcaaaa taaggtagaa ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa
    44221 aacacatcga tgccttattg gaagaatatg agatcaaagc aattgcggga acagttgatg
    44281 ttgattatca aaatatccca tttttttcag cctatgacgt ttttgatgat gaaaaactaa
    44341 atattttaaa gcgtatagct gatgatgaga tcccgacagc taaaattgtc gattcacttg
    44401 ctggatcatt aagcgatgtc ttttctattg atgaactgat ccgttcattg caaaagacag
    44461 ttcagcagat ccagaccgat ttgtcgatta tcgtggaacc tggagtagat actggaatcg
    44521 ttatccattt agccttcatg ttagacggat tgatcaaaca agaaagccat cggcaatttg
    44581 agaaactgtc tattttctca aaaatttatc gtttggaaat ggatatcgtt cgggcaaact
    44641 tgatggcact agaacgaaaa tatcaggtaa aaattccaga agatgatatc gcatttctta
    44701 cacaaatgtt tcttgaaaat aaaataaaaa aatgaacaca ctaatcatac tgtttacaca
    44761 ctaaacatct gtgtgcgcac tataaaaaag agctaaaaag catgaaaacg ctatttcagc
    44821 tctttttttt attggcatgc ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg
    44881 ttaaaaatgg caaaaaaaac aatcatgtta gtttgttcag cagggatgag tacgagttta
    44941 ttggtcacaa aaatgcaaaa agcagcagaa gaaaaaggga tggaagccga tatttttgcg
    45001 gtctctgcct cagatgcaga taataattta gaatctaaga atgtcgatgt attattatta
    45061 ggtcctcaag tacgtttcat gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt
    45121 ccattagatg tcatcaatat gcaagactat ggcatgatga acggagaaaa agttcttgca
    45181 caagcagaaa atctaatgaa ataaatcaag tatgcagttg ggggtataac aacgtggaag
    45241 atcaacaaaa tctagaagcc atcatggggt taatcatgtt tggcggtaat gcaaagagcg
    45301 atgcaatgga agcgattgcg gctgctaaaa tcggtgattt tgagttagcg gataggaaaa
    45361 tcgctgatgc agaagaatca cttatacaag cgcatcatgc acaaacagac atgttgacac
    45421 aagaagcaca aggaaatcat atgcaagtga cattattgac tgttcacagt caagatcact
    45481 taatgacatc tattgcgttt actgatttag caaaagaaat catcgaactt tatcgtcgcg
    45541 tatacagcga ataaatacaa cttctttttt gaacggaaga agaaaaattt ttgaacggat
    45601 tttttatttt gttataagga gaactctctt tatataaaaa acttatagga ggaagtaaaa
    45661 aatgaacgga ctatcaagtt ggatggagaa atacatcctg ccggttgccg caaaaatcgg
    45721 agcgcaaaaa catttagttg ctttgcgtga tgcttttatc ggcatactgc ccgcaacaat
    45781 ggcgggatca gtcgctgtaa tgatcaatgc aattattcgt gatttaccgc aacagtttaa
    45841 cagtacctat gctgacagtt tagcagcaga caaaggaatc tttgccgtat tgaatgtcat
    45901 catcggaatc aatggttttg tctggaacgg tacactagca atcgccggat tgatctttgc
    45961 cttttcttgg gggtataatc ttgcccgagc ttatggagta aatgatctag ctggggggat
    46021 cgttggttta gctaccttga ttcaaggtat ttcctttggt ccagcattat cagcagcatt
    46081 agatgtgaat gttccttcaa atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac
    46141 gattgcagat ggcacactgt cagttggatt atggggttgg ctaaaactag atcatttgaa
    46201 tggaaacgct tattttacag ttatgatcat gggagctatt tcagtcatta ttttttgtaa
    46261 attgatgctt gccaacatta cgatcaaaat gcctgattca gtaccaccag cagtatctaa
    46321 agcatttgca gccattttac cagcgacgat cgctttatac gtcattgcta ttatcaatta
    46381 tatcgtaggt cgtgtagctg atgggcaact gatcattgat ttggtgcaaa aatatattgc
    46441 agaaccattc ctaggattat ctcaagggtt gggagcagta ttgatcgtca cagtatttgt
    46501 tcaagtattc tggttctttg gtattcatgg atcaaatgta ttggcaccag tgctagaggg
    46561 aatctgggga caagcacaac taatcaatat tgatattttc caaaaaggtt ataacggaaa
    46621 aactggtaca ccagccgtgt tagatgcaat cgaagatgga aaagcttata tgtgggtccg
    46681 cggatcgttc gatgcttttg catggtttgg cggatcaggc ggaacgatcg tcttactgat
    46741 agctatcttg attttctcta aacgtgcaga ttatctaaca gtagctaaat tatccattgg
    46801 gcctggtatc ttcaatatca acgaaccagt gatgtttggt ctgccaatcg tattgaatgc
    46861 aatcatgttt atcccattct tgattgctcc agtagtagca acagcaattg gatggacagc
    46921 tacttatctt ggcctagtcg ctccggtatc gcagcaagtc acatgggtag taccgccgtt
    46981 cttattgtca ttcttagcaa caggagcgga ttggcgggca ccgatcgtaa cattagtatg
    47041 tatggtcgtg actttcttga tttgggcacc atttgtcatc tctgcaaaca aaatggaccc
    47101 tgccgcaggc gaacaacaat aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc
    47161 tcataatgaa ttttcgagga ggaactgaaa tgattacaat tgccagtgca atgcaacaag
    47221 atgaaatcaa agaattgtta gaaggataca atgaagaagg aatgactttt acatttaaag
    47281 aaaaacaagg aatcaagctg ttctttgaaa caagtgcaga agatccggaa gttgcggcta
    47341 aaaaagcaaa ggaactaatc aaagcacagc cttggggaac agtcctttat tttcaatcaa
    47401 cagctgttta gaaaatggtt taaaaagata ggatggttga gatgataaaa aacggtttat
    47461 ttttatgtag tattggttct ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt
    47521 atgatttgta tagtatggtc acaggtcttt ttctaacagg attaggaatc tatttcttca
    47581 tgaaggggaa aaaaagagaa gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa
    47641 attaaatatt ccagcatcgt ttttttacga taaagttatg gactctgtcc ttttcgatgt
    47701 acgtaaagcg accggcaaat cagtaactag aaaacagctg aaaaatttgg agtacgtcaa
    47761 acaattctcg caaaataacc gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc
    47821 ttataagttt cgtacatcga caacgaagaa tgaattcgtc gttcattatc aaatcaaacc
    47881 attggacgat tcgacctgtg aggttcatta cacagaaaaa atggagtctt atggcatgct
    47941 tcaaaagttg aatgatatgc ttttaggaac aatcttgatg tattttaaac ggagacgttt
    48001 caaaaaaatg ctagatatga tcgaagcaac gtattaaatc aatgaaacat ggtactattt
    48061 agttattgaa aaatgaagta agctagaagg aatttttcat aggaagatag atctttcctg
    48121 cgaatcctca ctagcttttt tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag
    48181 ctaggtgtat cttcgattga ggaaatgttt gatttagcca cttatgcatt taatggggca
    48241 gatacacctg aaaggcgaga acgttttcgt acacttgcgg agaattcttg gaattacgga
    48301 ttttttgatg aggaggatcg gctaacaagt caagtcatgg cgaccccttt tccagtgaat
    48361 ttttacggac aagagtacct aatggccggc attggctatg ttgcttctta tccagaagca
    48421 aggggacaag gcgggatcaa tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt
    48481 aaagtaagct tatcttatct ggcgccattc tcctatccct tctatcggcg ctatggctat
    48541 gaacaatcat ttgataaaat cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa
    48601 aaaaaatctg gaaaaataaa gcgtatggat tttgaagatg caaaagctgc aatcagaaga
    48661 atttatgaac agatgccgga aaaccaacga gctggtctga agcgcgaaga ctggtggtat
    48721 acgtataaat tcaagcaaaa aggcaattac cagtttgctt tgcattttga tgataatgag
    48781 gaagcaacgg gatatatcgt ttatcagctt gcaacgtctt cctttattat tgcggagtgg
    48841 ggatttttgg atcatgatgc tttttgcagc cttgttcgct tcgtaagttc tcacaatggt
    48901 gcttttgaaa catttgaata ttcatgcggc tacggcggta atgatcaaaa ttatctatta
    48961 gaaaatccat ttgcttctgt aatgatcaca ccttatatga tggcgaggat cgtagatatt
    49021 tctttgtttt tggaaaatta tccatttaaa aagagacaag ctgcatttgc cctacaaata
    49081 gaagaagatc attatgcgaa atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga
    49141 aaaaatcatc tctcaaaagt agaacaaaca aaactcccaa tcgttcgagg aagtatccaa
    49201 catatcacgc agctattatt agggtatcgt aaagtggaag agttggtatt ccaagaaaga
    49261 atacaggtga caaaagaata tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca
    49321 attcttaatg actatttttg ataaaaaacg ggtgatgaca atatttttgt caccaccctt
    49381 ttgtttttca tttggcattt gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt
    49441 tgaatttttt gttttctttt gttttagggt tttctttttt gtggtcttgg attgcttctt
    49501 tcgtaatctt ttcccgattt tttttagatg aatctaagcg ttctgcgtat actttttctt
    49561 ttggcttatc attcattgga aaggacctcc ctacattatt tcattctacc tctagtttat
    49621 catctgttaa ggactagtgg aaacgttttg cctaacttat aagctgattt ttttagcaga
    49681 taagcttgca agtaaaaaac aaacaagcta caattgattt attgaaattg ttcctgtagc
    49741 tcagtaggat agagcaatcg cctcctaagc gataggtcgc tggttcaatt ccagtcagga
    49801 acgtataaat aacccctgta tcctttgcgg tacaggggtt tgttttgtat ttgtgccaaa
    49861 aatgtgccaa actagttatc atttttatat aaactatcga acacagtatt gattttttct
    49921 ttatcctgat cttcaagctc tcgaacaata tgaagataag tagacattgt agtttctaaa
    49981 ctgctatgtc c
//
LOCUS       DASJUF010000007        45436 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJUF010000007 DASJUF010000000
VERSION     DASJUF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.007"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            447..1637
                     /locus_tag="U4B62_000375"
     CDS             447..1637
                     /locus_tag="U4B62_000375"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN2140560.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            1739..2692
                     /locus_tag="U4B62_000376"
     CDS             1739..2692
                     /locus_tag="U4B62_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN2140561.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            2761..4110
                     /locus_tag="U4B62_000377"
     CDS             2761..4110
                     /locus_tag="U4B62_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2140562.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(4293..4616)
                     /locus_tag="U4B62_000378"
     CDS             complement(4293..4616)
                     /locus_tag="U4B62_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140563.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            4745..5908
                     /locus_tag="U4B62_000379"
     CDS             4745..5908
                     /locus_tag="U4B62_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2140564.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            5978..7327
                     /locus_tag="U4B62_000380"
     CDS             5978..7327
                     /locus_tag="U4B62_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2140565.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7372..7803)
                     /locus_tag="U4B62_000381"
     CDS             complement(7372..7803)
                     /locus_tag="U4B62_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2140566.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            8191..10833
                     /gene="alaS"
                     /locus_tag="U4B62_000382"
     CDS             8191..10833
                     /gene="alaS"
                     /locus_tag="U4B62_000382"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN2140567.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            11033..11629
                     /locus_tag="U4B62_000383"
     CDS             11033..11629
                     /locus_tag="U4B62_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2140568.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            11751..12899
                     /locus_tag="U4B62_000384"
     CDS             11751..12899
                     /locus_tag="U4B62_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2140569.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(12937..13350)
                     /locus_tag="U4B62_000385"
     CDS             complement(12937..13350)
                     /locus_tag="U4B62_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN2140570.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            13429..13860
                     /locus_tag="U4B62_000386"
     CDS             13429..13860
                     /locus_tag="U4B62_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN2140571.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(13959..14192)
                     /locus_tag="U4B62_000387"
     CDS             complement(13959..14192)
                     /locus_tag="U4B62_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN2140572.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14375..16045
                     /locus_tag="U4B62_000388"
     CDS             14375..16045
                     /locus_tag="U4B62_000388"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN2140573.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16192..16578
                     /locus_tag="U4B62_000389"
     CDS             16192..16578
                     /locus_tag="U4B62_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140574.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            16565..17263
                     /locus_tag="U4B62_000390"
     CDS             16565..17263
                     /locus_tag="U4B62_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2140575.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            17268..17732
                     /locus_tag="U4B62_000391"
     CDS             17268..17732
                     /locus_tag="U4B62_000391"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN2140576.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            17729..18640
                     /locus_tag="U4B62_000392"
     CDS             17729..18640
                     /locus_tag="U4B62_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2140577.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            18875..19414
                     /gene="pyrR"
                     /locus_tag="U4B62_000393"
     CDS             18875..19414
                     /gene="pyrR"
                     /locus_tag="U4B62_000393"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN2140578.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            19428..20711
                     /locus_tag="U4B62_000394"
     CDS             19428..20711
                     /locus_tag="U4B62_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN2140579.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            20724..21650
                     /locus_tag="U4B62_000395"
     CDS             20724..21650
                     /locus_tag="U4B62_000395"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN2140580.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            21669..22949
                     /locus_tag="U4B62_000396"
     CDS             21669..22949
                     /locus_tag="U4B62_000396"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN2140581.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            22954..24030
                     /gene="carA"
                     /locus_tag="U4B62_000397"
     CDS             22954..24030
                     /gene="carA"
                     /locus_tag="U4B62_000397"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN2140582.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            24035..27220
                     /gene="carB"
                     /locus_tag="U4B62_000398"
     CDS             24035..27220
                     /gene="carB"
                     /locus_tag="U4B62_000398"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN2140583.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            27274..28050
                     /locus_tag="U4B62_000399"
     CDS             27274..28050
                     /locus_tag="U4B62_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN2140584.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            28047..28979
                     /locus_tag="U4B62_000400"
     CDS             28047..28979
                     /locus_tag="U4B62_000400"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN2140585.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            28976..29692
                     /gene="pyrF"
                     /locus_tag="U4B62_000401"
     CDS             28976..29692
                     /gene="pyrF"
                     /locus_tag="U4B62_000401"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEN2140586.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            29699..30328
                     /locus_tag="U4B62_000402"
     CDS             29699..30328
                     /locus_tag="U4B62_000402"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEN2140587.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30316..30630
                     /locus_tag="U4B62_000403"
     CDS             30316..30630
                     /locus_tag="U4B62_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140588.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            30668..31309
                     /locus_tag="U4B62_000404"
     CDS             30668..31309
                     /locus_tag="U4B62_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2140589.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31352..32044
                     /locus_tag="U4B62_000405"
     CDS             31352..32044
                     /locus_tag="U4B62_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN2140590.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            32041..32943
                     /locus_tag="U4B62_000406"
     CDS             32041..32943
                     /locus_tag="U4B62_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2140591.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(33101..34807)
                     /gene="efbA"
                     /locus_tag="U4B62_000407"
     CDS             complement(33101..34807)
                     /gene="efbA"
                     /locus_tag="U4B62_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN2140592.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            34997..35620
                     /locus_tag="U4B62_000408"
     CDS             34997..35620
                     /locus_tag="U4B62_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140593.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            36070..37119
                     /locus_tag="U4B62_000409"
     CDS             36070..37119
                     /locus_tag="U4B62_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2140594.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            37116..37997
                     /locus_tag="U4B62_000410"
     CDS             37116..37997
                     /locus_tag="U4B62_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140595.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            38000..38770
                     /locus_tag="U4B62_000411"
     CDS             38000..38770
                     /locus_tag="U4B62_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2140596.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            39049..39903
                     /gene="aroE"
                     /locus_tag="U4B62_000412"
     CDS             39049..39903
                     /gene="aroE"
                     /locus_tag="U4B62_000412"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN2140597.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            40081..41244
                     /locus_tag="U4B62_000413"
     CDS             40081..41244
                     /locus_tag="U4B62_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2140598.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41361..41771
                     /locus_tag="U4B62_000414"
     CDS             41361..41771
                     /locus_tag="U4B62_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2140599.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            41945..44173
                     /gene="topB"
                     /locus_tag="U4B62_000415"
     CDS             41945..44173
                     /gene="topB"
                     /locus_tag="U4B62_000415"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2140600.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44344..44628
                     /locus_tag="U4B62_000416"
     CDS             44344..44628
                     /locus_tag="U4B62_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140601.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            44788..45024
                     /locus_tag="U4B62_000417"
     CDS             44788..45024
                     /locus_tag="U4B62_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2140602.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            45021..45420
                     /locus_tag="U4B62_000418"
                     /pseudo
     CDS             45021..45420
                     /locus_tag="U4B62_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT        14903 a         7880 c         9864 g        12789 t
ORIGIN      
        1 tattcggaag aataggactg tgacaagact tttgtcacag tccttttttc gaattcaaca
       61 agtaaaactt ttaagaaata ttcagagagt aaaattaaaa attgctgata aacgaagatg
      121 gaaattttat gtatttttat tggtctatgc aataaataaa ttaaatattc agaatacaat
      181 tgattttaac gaaagggctt ctatcaatgg ttgtgttgta tttatctgcc caggtagagt
      241 aaaatagatc attatctaaa aggtaattgt tcatactata taagtcattt gaaagtaaaa
      301 agatactgta aataaaaaat gtggttaaat aattctcata atcagtgtaa gcgttatcaa
      361 aaatatagca tacttgcttt gtagatgaat gacacgatat atgtttggta tgttctgcaa
      421 gacagatgaa tatggaggtg gagacaatgt cttccaaaga actaacaatg tatttagaga
      481 aagaattgga tcaactgaaa gaaaaggggt tatacaatac gatcgacata ctagaaagtg
      541 aaaatggtgc ttgtatcatc gtagacggaa aaaagatgat caatctagca tccaacaatt
      601 atcttggatt tgccaatcgc gaagaattga aaaaagcatg tattgaagca accgaaactt
      661 atggtgtagg agcaggagca gtccgaacga tcaatggttc gctgaaaatc catcagcaat
      721 tagaagaaaa aatcgcagaa ttcaaaggga cagaagcagc aatcgccttt caatctggat
      781 tcaactgcaa tatgggcgct atctctgcag taatgacaaa agaagatgct atcttatcgg
      841 atgaattaaa ccatgcatct attattgacg gttgccgctt atccggagca aaaatcatcc
      901 gaattaaaca tcaagatatg aaagaccttg agaaaaaagc aaaggaagcg atagaaagta
      961 agaaatataa aaaaatcatg tatatcacag atggtgtttt ctccatggat ggagacattg
     1021 cacgattgcc ggaaattatt ccgattgttg aaaaatatgg actgatcacc tacgtcgatg
     1081 atgcgcacgg ctcaggcgta actggaaaag gtgcaggaac cgtcaagcat tttggattaa
     1141 gcgacaaaat cgatatgcaa atgggcacgt tatcgaaagc tataggtgtt gttgggggtt
     1201 atgtagctgg atcgaaaaca ctaatcgact ggttgaaagc tagaagtcgg ccattcctat
     1261 tttctacttc gctaactcca ggtgcagctg cagctgcatt ggcttcgatt actttgatgc
     1321 aagaacatcc ggaacttgtc gaaaaagttt gggaaaatgc taattatttt aaagaagaat
     1381 tgaaaaaagt aggctataac atcggtatgt ctgaaacacc tattactcca gtcattttgg
     1441 gagacgaaaa agtgactcag actttctcta agaagctgat cgaacatggg atttatgcta
     1501 aaccaatcgt ttatccaact gtgcctcttg ggacaggcag gatcagaaat atgccgactg
     1561 cggaacatac gagggaaatg cttgatgaag cagtagcagt ttatcaaaaa ataggaaaag
     1621 aaatggaaat tatttaacta aatttagttt tttcgtctat ttaaaattag agaacatgtt
     1681 cacgcatata ttgttaatca tctgaagata gtagtaaaaa agatcaggga ggatcatcat
     1741 gaaaaaagta ttggtgacag gatgtttagg acaaattggt tcagagttga tcacacggct
     1801 acgtcaagaa ctaggcagtg aaaatgtcat tgcgacagat atacgcatgc ctgaagtcat
     1861 tgagaaaaac gatatctttg agatcctaga tgtaatggat tacgaaaaaa tgcgcagcat
     1921 agcagaacaa tatcaagtgg acacattgat tcatttggct gctcttttgt cagctgttgc
     1981 agaagcaaaa ccacaattag cctggaattt gaacgtgact gggctggtca atgcattgga
     2041 agtcgctaga gaattccagc tgaaattttt tacgccaagt tcaatcggcg cgtttggtcc
     2101 aaatactcca aaagatcata ccccacaaga tactcttcag cgtcctacaa ccatgtatgg
     2161 ggtaacgaag gtagctggag aactactatg tgattattat tatgtgaaat atggcgtaga
     2221 tactcgtggt gtccgtttcc ctggattgat ttcttatgca accttgccag gaggcggtac
     2281 gacagactac gcagtcgata tttattattc agcaatcaaa gaaggaaagt acactagttt
     2341 cattgatcaa ggtacatcaa tggatatgat gtatatgcca gatgcgatcg atgccattat
     2401 aaaactcatg aatgcagatc ctgatcgatt ggttcatcga aatgccttca atatatcggc
     2461 gatgtctttt gacccggaag agatcaaagc atctattcaa aaaatcatac cagattttga
     2521 aatggattat gatgtggatc ctgtacgtca gtcaatagca gaatcttggc caaattcact
     2581 agatgtttct tgtgcgcaag aggaatgggg cttttcgccg aaatacgact tagatgtgat
     2641 gacaaaggat atgttggaaa aactgaaaga aaaattggta gagacggtat aaaacaagaa
     2701 ccacatccaa aaagtttttt atacttttgg gattggctat ggtatgaaag gggacaaggg
     2761 atggaagaat atcaagccac gccagtaaaa gaagtaaaaa tagaaaatga attaaaacgt
     2821 acgatgggat tttttactgc tttatcgact gttatgggca cagtgatcgg tgcaggggta
     2881 ttttttaaag ccgcaagcgt agcagaagtt acaggatccg ccagtttgca tatgttttct
     2941 tggtttctcg gtggtatgat ttctgtctgt gcaggactaa caggagctga actggcagct
     3001 gctattccag aaacaggagg gatgattaaa tatatcgaac ggatttacgg aaatacagca
     3061 gcttttttat tgggctgggc gcaagtcgtg atttattttc cagcaaatgt agcagccttg
     3121 tcgatcattt tcggtacaca gttcgtcaat ctatttggtc tttcccagtc aatgattgtt
     3181 cccgtagctg ttacagctgc tgtatcgatc ctgttgatca attttttagg ttctaaagct
     3241 ggaggggctt ttcagtcgat tacgcttgtc tgcaagctta ttcctttgtt tgtgatcgtc
     3301 attttcggtt tgttccgaca agaaggagta gacttccaac tgtttccgat ccaagcagga
     3361 gaaaatcttt catttttctc agccttggga gcgggcttgc tagcaacgat gttcgcatac
     3421 gacggttgga tacacgtagg aaatatttcg ggagaattga aaaagccggc gaaagatttg
     3481 ccaaaagcaa tttctttagg aatcatcgga atcatgatcg tttatttatt agtaaatgcg
     3541 gtgttcttaa gaaccgcatc tatcgacgga gtcgcaggaa acagcaatgc ggcaagtgac
     3601 gttgcaaaaa tgatttttgg cggcttcggt ggaagactgg tcacagttgg tatcttgatt
     3661 tctgtttatg gaaccatcaa tggctatacc cttactggaa tgcgtctccc ttatgttatg
     3721 gcaaaagaaa acaacctgcc ttttagcaag ctctttgcta aacttcatga taaaactaaa
     3781 gttcctgtag ccgcaggtat cttagagcta gtcatcgcta ttggcatgat gatgatcggt
     3841 ggttttgata cactaacgga tatgctgatc tttgtcattt ggatttttta tacgatggtc
     3901 tttgttggtg tcattcttct tcgtaaaaaa gaacctgatt tgtttcgacc atacaaagtt
     3961 ccaatgtatc cgtttattcc actagtagca atcattggag ggacttttat cgtatcaagc
     4021 acgcttatca ctcagacctt tttagcttcg atggggatag tattgacact tgctggtatc
     4081 ccgatttatc tctatttaaa acgtagatag ctaaaacatt cgaattttat tagaactgca
     4141 cttctctgcc aaacaaaata ttttatttaa atgaggagta cgtggaagaa atgtcaaaag
     4201 tactcgttaa ttttgatcac gtaaatgtcg ttttgtctac gatgaaatag caaagtaaaa
     4261 gacagaaatc gggctcaacc gatttctgtc ttttactttg atttgcgtaa ctgaactatt
     4321 cggcgtacgc cgcgataaat atttaataaa accaagcaac cagaaatgaa aatgggaagc
     4381 caaatcagat agacagggta gccaagcact ttagacaaag catgtggagt cattttatcc
     4441 atttcaagaa gtgcttgctg ccaagtctga agattgaaga ctagcatgaa acccgaaata
     4501 atcaggagaa taaatcctaa ataatgaatt gtagttcttt ttcttacata agcgatcgta
     4561 gcataaagga acagtaaaaa aataggtacg ccaaaaacaa agtacatatt cagcatctcg
     4621 acaccccctt tgtgtttatc ttactttact ttgaaagaaa tgagaaataa tagcctcgtt
     4681 acttgcttga aacagcaaag gactgctaag ataagagaga tacaaagaat caaggaggag
     4741 tcatatggca agttatatcg aaccgattcg ttttgcgctg attgtttttc catttttagc
     4801 cttagcgatc tcatctgttt tttttattta tgaatatcga aagtatggaa catttctagt
     4861 aacaagggca gttattttgt attctttcgt tttttattta ttgtgtgcgt attttttggt
     4921 gatcttacca cttccggcaa aggagacggt tgcccaaatg acagggccaa agatggagct
     4981 gcatttattt gcttcatggc aacatttcag agacaacacg gtattggtct tgtctgaacc
     5041 tagtacctac ctgccggcaa tgaagcagag tgtatttttg gaaccagctt ttaatgtttt
     5101 attactcata ccgtttggca tttatttaag atattatttc cggctttctt ttatgaaaac
     5161 gatgattgct agtttctgcc tttctctatt ttttgaattg acgcagctaa gtggacttta
     5221 ttttatctac ccaagaccat accgcttatt tgatgtgaat gatttagtga caaatacatt
     5281 gggtggaatc attggttttg tcgttacgcc gttatttaca tttatgcttc ctacaagaag
     5341 acgaatggat gaactttctt atgaaaaagg aaagactgtc tcattgactc gacgcttggt
     5401 cgcttatttg atcgactgga catttttatc tcttattaat ttggtagcgg cgattttctt
     5461 taaaattgcg ttcgattcag gggcagttac ttctcatatc tggtggcttt tattagaagt
     5521 aggggtatac tttatgattg taccgtttat aaccaatgga tggacacttg gcaaaaaaat
     5581 tgtgcgtatc cgtttggtgg aagaagaccg cgaacgaatt tctttaaaag cattgatgat
     5641 ccgctacggg tatctgtact ttctttttta tggtttaagc tggttggggc agttgcttca
     5701 gcaaatcatg aattcgacaa cgaatacggt gttgcagatg agtgcagtat tcatttctct
     5761 cggtgtcttg ttactacaag gtatttttat tttaaatatc ttgctatcat ttttgagaaa
     5821 aaaacggatt ctgttttatg aaaaagctag tcacacatat actgttagca cgattggcca
     5881 aacaaaggat gatgattctc gaaattagct gagcaaaaac gctttgtctg tgttataatg
     5941 ccaaagtcgt gataatttaa agagaagagg tgcacgaatg aataattttg atatattccg
     6001 cttcaaaccg tttatcaatg aagcgttaac cgcaaagggc ttcaaacagc ccacagaggt
     6061 ccaggaacgt ttgattcctt taattgcaaa aggaaaaaat atcattgggc aatctcaaac
     6121 tggtacagga aaaacacata cgtttttgtt gcctttgttt gaaaaaatca atgcagaaaa
     6181 agaggaagta caagttgtta ttacagctcc aagtagggaa ctagctgcac agatctatga
     6241 agcagctaca caaatcgcta aattttctac accggagatc cgtgtctcga attttgtcgg
     6301 cggtacagac aaacagcgtc aattaaatcg tctgcatcac caacagccac aggttgtagt
     6361 aggcacgcct ggtagaatat tagacatgat gaatgaacaa gcgctgaaga ttcacaccgc
     6421 atttgcattt gttgtcgatg aagcggatat gacattggat atgggttttt taaatgaagt
     6481 agaccaaatt gcaagtcgat taccagaaaa gctgcagttt ttagtttttt ctgccactat
     6541 tccagaaaaa cttcgtccat tcttacgaaa atacatggaa aatccaatca ttgaagaaat
     6601 caaaccaaaa gcagttattt ctgaagacat tgaaaattgg ctgatttcaa caaaaggtaa
     6661 agatgagaat caaatgattt atcaactgct gacaattggt catccttatt tagcaatcgt
     6721 atttgccaat acaaaacagc atgtggatga aattgccgat tacttgaaaa accaaggctt
     6781 gaaagtagca aagattcatg gagacatcac cccaagagag agaaaacgag tgatgcgaca
     6841 agtacagaat ctggagtatc aatatgttgt agcgactgat ttagctgcta gagggatcga
     6901 tatcgaaggt gtctctcatg ttattaatgc agaaattcca gaagatctag acttctttat
     6961 ccatcgtgtc ggacgtactg gacgtaatgg cttaaaagga acggccatta cgttatattc
     7021 gccaaatgat gagcaagcca ttgatgcgat agaaaaattg ggcgtctcat ttgaaccaaa
     7081 agaaatcaaa aacggggaaa tcgtcaaaac atacgatcgt aatagacgtg ccaaacgaga
     7141 aaaatcaaaa gatgtattag atcccacgct gattggtctt gtgaagaaga aaaagaaaaa
     7201 gatcaagcct ggttataaga aaaaaatcga ttgggcaatt gcagaaaaaa ataaaaaaga
     7261 acgcaaaatc gaacgcagac agcaaagccg tactgctcgt aaaaataaga aaaattcaca
     7321 taaataaaaa acaagaggtt gtgacaaaag gcgtgtcaca atctcttgtt tttaagctag
     7381 actttttttc attccgatac aaagtacttc atcttcaata aaaggttctg tgcaagtatg
     7441 atacccttgt ttttcataaa aatctacagc cgtcatttca gctactacat aactagtctg
     7501 acaaccttct gaaaccgccc gtttttcagc agcttcaagc aactgaccgc ctaacccttt
     7561 tcctcggtat tcttttgcta cgcaaaatcg ttccggcttg aagcaagtag gtgttttctt
     7621 ttcataacgc aaagtagcta ctggtctttt ttgatcaaga aatagataat aggtttgttg
     7681 cgggatatct tttccgtcga attctagttc tgcaggaatc ccttgttcta atacaaaaac
     7741 ttgatatctc aaaaagcagc tagctgcgcg tatccattca tccgctccaa ctattttttt
     7801 catcatattc ctccttgtca tgaatagcag agatgtattg attcctttat tcttatcgta
     7861 ccaaaaatcg tggagtatga ccaaaaggaa atgaaaataa aaattgagat attgacagtg
     7921 atcaatagaa tgcgtatact aattaagtac gaaaaggata tgatttgttt tttaagactc
     7981 acagaaaatt ctttcttggc tgaaaaaaga atagtcacta atcaaattgc tccctttttc
     8041 tattttggta gaaataccga cgcagctcag cgttatcgag tttaagaggt aatgattaca
     8101 catcattgca atcaaggtgg taccgcgagt tttcgtcctt ggctgtgatg gtgttttttt
     8161 gtatttaatt tatagaaagc aggtaatgac atgaaacaat taagtagtgc acaagttcga
     8221 caaatgtttc ttgatttctt ccaatctaaa ggacatacga ttgaacctag tgcgtctttg
     8281 atccccgtga acgatccaac cttattgtgg atcaactctg gtgttgcaac attgaaaaaa
     8341 tattttgatg gttcagttgt gccagaaaat ccaagaatca caaatgccca aaaatctatt
     8401 cgaacaaatg atatcgaaaa tgtcggtaaa acagcacggc accatacgat gtttgagatg
     8461 ctgggtaatt tttcgattgg tgactatttc aaaaatgaag ccatccactg ggcatgggaa
     8521 tttttgactg atccaaaatg gatggcattc gatccagaaa aactttatgt aacagtttat
     8581 ccaaaagata cagaagcaaa acgcatctgg catgaagaag ttggcctgcc actagatcac
     8641 attattgatg tagaagacaa cttctgggat atcggtgcag gcccatgtgg tccggatacg
     8701 gaaattttct atgaccgtgg agaagcttat aacgatttag cagaagacga tcctgaaaac
     8761 tatccaggag gagaaaatga acgttattta gaaatctgga acttagtatt ttcagaattc
     8821 aaccataagc ctgataacac atttgaacca cttcctcata aaaacattga tacggggatg
     8881 ggattggaac gcatggtctc aatcgttcaa gatgcgtcaa cgaactttga gacagacttg
     8941 tttatgccta ttatccacgc cgtagaaaag ctaagcaaac aagtaaaata tggtgaaaac
     9001 ccagtaacag atatttctta caaagtaatc gcagatcata ttcgcgccct ttcatttgct
     9061 attggcgacg gtgcattgcc atcgaatgaa gggagagggt atgtcttacg ccgtttgctt
     9121 cgtcgtgctg tcatgcatgg gaaaaaatta ggtattgaac aagcattctt atatcgttta
     9181 gtccctgttg ttggggaaat catggtaagt tattatcctg aagtacttca acaaaaagaa
     9241 tttattgaaa aagttatccg tacagaagaa gaacgtttca atgaaacaat caatgaaggt
     9301 ttggaaatct taaatcaagt gatccaagaa gtaaaaaacg aaaacgggac gactctagca
     9361 ggaaaagaca tcttcaaact atacgacaca tatggcttcc cggtagaatt aacggaagag
     9421 gtggcagaag atgaaggact gaaagttgat catgctggtt ttgaaaaaga aatggatgca
     9481 caacgcgaac gtgctcgctc tgcccgcagt actgaaaaat caatgggggt acaatctgct
     9541 ttattgacag atatcaaagt agaaagtaaa ttcattggat acacagaaca agaatcagat
     9601 agtcaattat tggtcatcgt taaaggtgag tctcttttga atgaagtcgc agaaggtgaa
     9661 gctcaaatga tctttgatca aacacctttc tatgcagaaa tgggtgggca agtagcggat
     9721 caaggatgga tcatgaatga agtaggcgaa atcgtagcag ttgttacaga tgttcaaaaa
     9781 gcgcctaatg gtcaatttat gcacaccgta gaagtaaaag caccaatgag tgaaggacaa
     9841 acctatcatc tattgatcga tcaatcgatg cgcaatcgga tcatcaaaaa ccatacagca
     9901 acccatctac tgcacaaggc attgaaagaa gtacttggcg agcatgcaaa tcaagctggt
     9961 tctttagtcg ctcctggaca tttacgattc gactttaccc actttggtca aatcacttca
    10021 gatgaattag ctgaaatgga aagaatcgtc aacgaaaaaa tctgggaagc tatcccagtt
    10081 gaaacaattg aaacagatat cgatacagcc aaaaacatgg gtgcaatggc tttgtttggt
    10141 gaaaaatacg gacatgacgt tcgagtagta aatattggcg gctggtcaat cgaactgtgc
    10201 ggaggcacac atgtagcaaa tacagaaaac atcggaatct tcaaaatcgt ttctgaatca
    10261 ggaattggtg caggcgtgcg acgaatcgag gctgtaacaa gtaaagaagc gtacgaacta
    10321 cttgaagaag aagaaaaaca actaaaacaa gtagcaacaa ttgttaaatc acctcaattg
    10381 aaagaagttg tttcaaaagc ggaacaattg caacagcaag taagagagct acaaaaggaa
    10441 aatgaacaat tagctagcaa gttagcgaat caacaagctg gtgatgtatt caaagatcca
    10501 gaaacgatca atggaaaaac aatcattgca gcacaagtaa aagtgaaaga tatgaatcag
    10561 ctgcgtcaat tagctgacca atggaaacaa aaaaatattt cagatattct tgttttagca
    10621 aacgaacaag aaggaaaagt cagtctgcta gcggcaatga cgaaagaatc aaatgaagct
    10681 ggactaaaag ctggcgactt gattaaagca atcgcaccga aagtaggcgg tggcggtggt
    10741 ggacgtccag acatggcaca agctggcggg aaaaatcctg ctggtattcc agatgcactt
    10801 aaagctgcga aagaatgggt tgcgcaacaa tagggcgttt acgtttgaca gaaaaacaaa
    10861 agttcaaaca ttgaacgaaa catgattatg ggggcgaaga caaacaagtc ttgccctcat
    10921 acttttttca attggatggc gcatctaatc aaatggtcgg attcggatgg tctttagttg
    10981 acgaaagtga cagaaatttt tacgattagt ataaataaaa aggggtgaaa aaatgaatag
    11041 tagaaataag acctttcgac tagtcttacg tgcgatcttg ttagcaatca tcatcgtaca
    11101 agcgatggtt ccttggctgg ggtttattcc attaggattc attagtttaa caattatcca
    11161 tatcacagta attattgctg cagtagtatt aggcccaaaa gatggcatgg tgattggctt
    11221 gttttgggga atcgctacaa ttgttcgtgc ctatgccatg ccaacaacgc catttgatac
    11281 attagtgttc acgaatccga ttatctcggt agttcccaga gtacttgttg gcttagtagc
    11341 aggcttggtt ttccactgga tttatcaaaa aaataaatca ataacgatca gctcagcctt
    11401 tgcaggggta ctgggttcat tggtcaatac agtcttagtg ttaggattca tgggattatt
    11461 ttatacaggt gcaacggcag atgcctatgg tgtagactct gctcttttat ttaaaacctt
    11521 agcaggggtt gcagcaatca atgggatacc agaagcaata ggtgcgggaa tcataacacc
    11581 attactagca aaagcgttat ttgcagcaac accgttgaaa ccagaataat gaccatcttt
    11641 taatagtaat aggacgcacg atagatagaa aaaggaagag aggagaggaa aacaatcgtc
    11701 ctctccatct ttttacctga gcaacaagca aactagaagg ggtaaaaaac ttggctaaga
    11761 aacaagaaga aaaaaagaaa caaataccgc aacagcggac aaaaaaagga gtactagcgg
    11821 gtatcttagg attggttacg aatctgatct tgtttgtctc taagctaatg atcgggcttt
    11881 tttcaggaag tgtctccatt atggctgatg cagtgaacag tctatcagat actgcttcat
    11941 ccatattgac tttgatcgga tttaaaattg ctgcaaagcc agcagatcag gaacatcctt
    12001 atggacacga acgcttcgaa tatatcagtg ggttatttgt ttcgatcatc attacttatg
    12061 taggatttca atttttagag tcttctgctg aaaaaatcat ccatccagaa aatgtctctt
    12121 taacacccat cgtctttttt gtcctaatca tttccatctt tttgaaattt gcacaaggca
    12181 aaatgtatca ggttattgcg caatccattg attctgaaac attgagagct acagcaacag
    12241 atagttataa tgatgtcttt actacactgg ctgtattgct ttcagcagca atcgaatggg
    12301 taactggatg gcgtattgat ggatatatag gttttgcttt agcattatat atcctttata
    12361 gtggattgac gatgcttcga aattttgtat acgaactatt aggaagtcgc ccaacgccag
    12421 cagagattga agaaatggaa gagcatttaa atgcttgcga tgatatactt ggctatcatg
    12481 atttattggt gcataactat ggacccaata aacgctttgc atcagtccac atcgaagtag
    12541 atgaatcaat gaatttgaat gtggctcata agattattga tctgatagaa aaagacttta
    12601 aaaagtcttt ggatgtggaa cttgtctgtc acttggatcc aatgcctgtt gataacgaaa
    12661 aatactataa tatcttgcgg cagttaaagc aaattcttct tgaaacaggt acagggctta
    12721 atattcatga ttttagagta attcatcaag gaaaaacgct gcaatttgat gtagtggtcc
    12781 ctgagaaatt ccagtttgtt gatacagaac tagaaagaat cttacgtagt caaatagaag
    12841 aaaaaatcgg agattaccaa ttggaaatta cgtttgatca caattatctt ttatattaaa
    12901 tgagaaacta taaaaagact ccttaaagtt tttaatttaa agagtctttt tttcttttaa
    12961 ttgtttatgg agagcctgac gtgcaagatt atctgctcct ttatttttgt tttcgggcaa
    13021 ccattgcagg atcaataaag aaaattcgga tagaagttct tggattcttt gaaggtaggg
    13081 aaggaagagc tcgttcttcg ttgcgttttt gtcgaccgta gaaactagtg ttttgctgtc
    13141 tgaaaaacag aaaatcgttt cattctgtaa gtcgtttttc tttagaaatt ctaaagtttt
    13201 taaaaagact tcgaactctg cttgatggtt tgtccctata gaaagcggga tagatagctg
    13261 ttcttgttta ccttgataga caatcaagat tccacctcca ctaggaccag ggttgccttt
    13321 tgtagaggca tcaatgtata cttttaacat aaaaagagat tctccttttt gttcaaacta
    13381 atgttatggt aagattatat cattactgaa tcggtgagag gttagagaat gaaaaaaaat
    13441 attcgatggc agcctgaact ggcacatacg atcatctatt ggtcattgac cttgatcatt
    13501 ctgtttttta gtctgattct ttctttagaa aacacaggtc cttattggaa aagcaatttt
    13561 gttttaggta tttttcttgt ttttctattt ctaggtttgc ggcgttcttt tattttaaaa
    13621 tcaaactctg tgattatacg ttatgctgct ttttggaaag accgagaaat atctgtcgga
    13681 cagattcgat ctttgacgcc aactaatact ggggtgcaaa tgatgttgca agatgagaaa
    13741 gccccatctg ttttcatgat gcgaaaaaaa gatcaagtag tcctttttga tcattttgaa
    13801 aaaaacggac ccaaatcaga atcaattcct tatccttata cgaaaaaaag aaaaagctaa
    13861 ttttaaaaat cagaacctaa aaaaacgaat ttttacagga aaagaaaaga gtgagacgta
    13921 cggttcatcc cgtttagcgt ctcactctat ttgtttatct attcatgcat actagtatcg
    13981 gtagtctgaa cgttgccttt cttgacatgg gcagcttgtg ttcctcgatt cccttcgatg
    14041 atctcaaatt cgaccacttg attttcttcc aaagttttga atccctcttc ttcaatcgca
    14101 gtaaaatgta caaaaatttc ttctgtgtca tcataactga taaagccgta tccttttttg
    14161 ttgtcaaacc attttacaat tcctgtggtc aaagcactca cgacctttct aaagtctgat
    14221 catagccttc attataaaga gtatttataa gtacgtcaaa taaattaaaa acctgtaatt
    14281 ttatgttttt tttctgaata aaaaaaggta taattggcat atcttaacaa agaagatatt
    14341 ttgttgaata aagttcgtga aaaggggtaa tcaaatgaag aatgatctgc aaatttcaca
    14401 agaaacagag ctagaaccga ttgttgaagt ggctagtaaa ttagatctta aggaagacga
    14461 tttggaactg tatgggaaat ataaggcgaa aatcaatttt tctggaatca atcgatcaaa
    14521 ggataacgct gaggggcatt tgatcttagt tacttccatc aatccaacac cagctggcga
    14581 aggaaagtca acgatcactg tagggttagg agacgcgctc aataaaatca ataaaaaatc
    14641 tgttattgca ttgcgagaac cctcacttgg tcctgtgatg ggaatcaaag gtggtgctgc
    14701 aggcggagga tatgcacagg tattgcctat ggaagatatc aacttgcatt ttactggtga
    14761 catgcatgct atcacaacag ctaataatgc attatctgct cttttagata atcatattca
    14821 tcaaggaaat gaattgaaca tcgatgcccg ccgagtgatt tggaaacggg tagtcgatct
    14881 caatgatcgt gaactgcgta aagtaattgt tggcttgggt gggccaatcc aaggtgttcc
    14941 aagagaagat gggtttgata tcacagttgc cagcgaaatc atggctatct tgtgtcttgc
    15001 ggcagacctt gaagatttaa aggctcgtct tgcaaggatc gttgtcggct atacatttga
    15061 ccgtcagcct gtgactgctg gagacttgaa agcagaaggc gctctggcat tactgctgaa
    15121 agatgcaatc aagccgaatc ttgtacaaac aatttacgga acaccagctt ttgtccacgg
    15181 gggaccgttt gccaatatcg ctcatggctg caatagtatt ttagctacca agacagcatt
    15241 gagattagca gattacgttg tcacagaggc aggatttggt gcagatcttg gcggagagaa
    15301 atttttagat atcaaagtac ctaacttgaa aaaagcgccg gatgctgtgg tgattgtcgc
    15361 cactgtccgt gcattgaaaa tgcatggcgg aatgaaaaaa gatgaactta aaaatgagaa
    15421 tcttgatgct ttgaaagtag gttttgcaaa tttaaagcgc catatacgta atatggaaca
    15481 atatcagcta cctgtgatcg tcgcaatcaa tgaattcgta acagatacag acaatgaact
    15541 cacactgttg gagcacttgt gtgaaaatca ggggattctt gccaagagag cgagtgtatg
    15601 ggcagatgga gcagaaggcg gcgttgattt agcaaaagct gtagtacgat tgattgatag
    15661 aaaagaagct aattacaagc cattataccg cctagaagaa acgatacaag aaaagacaga
    15721 aacaatcgtc aagaaaattt acggaggaaa tggtgttgtt ttcagcaaaa aagcgcaaaa
    15781 acaaatagaa gagtttacga agaatggatg ggacaaactg ccgatttgta tggcaaaaac
    15841 acagtattca ttttctgatg atccttcact gctaggtgca ccggaagatt tcaccatcac
    15901 gatccgcgaa atcatcccaa aattaggggc aggattttta gtagccttga ctggagatgt
    15961 tatgacgatg cctggtttgc ctaaaaaacc tgctgcattg aatatggacg tgacaaatga
    16021 cggagaagtg ttgggattgt tttaagcttg attataatct accatcgaag gagctgtatt
    16081 ttgactacag cttctttttt tagacgatag ggaattaatt ttgtttacac taggatcatt
    16141 ctttttcttt attgtatttt gagctatact aaagcataat aggagggtat gatgaaaacg
    16201 acatttataa gaaaaaccgg tttttacggt atgggtagct cgattcagct aagaatcaac
    16261 aatgaaaaac gcagtgttct cagtcatgaa caaaagataa cgcttgatct ggaagtgccc
    16321 tttacgatgc aggtcacctt ttattggcta aaaagccccg tttatacagt gaaagaagca
    16381 aagcctgctt atgtgatcac aatgaattac ttgattcttc aaatctatcc gtttttattt
    16441 ctagcaactg ggttgggggc cgttttccta caaaatttat ttttcagttt ttttgcagtt
    16501 attgtgttga ttgcgttctt tttctatata aaaaaccggg catatttgat caaggaggaa
    16561 aattatggca aactctgatc tttttttaat cagtttcaat cgaatcgaaa aatggatgag
    16621 agacgagatg ggaaatgcac gaaatatggg atttaccgaa cttgtcagac gcctagctca
    16681 aagaaaacaa ttaatgatca gaaaatacga agatgatctt ttacagcttg ctcagttaag
    16741 gaatgcgatc gtccatgatc ggatcgcagt cgattttgtc attgcagagc caaatgattg
    16801 gacagtcaag cgaatccagc tgatcgaaca agaactgatt cagcctgaaa ccgtgctgcc
    16861 tcgatttgca aaacgtgtaa caggttttga acaagatctt ccgttacttg aactgttaaa
    16921 gatagtagca gaaaaaaggt attcacaatt tccgttatac aataaaggaa aatttgtagc
    16981 attgattact ttacgtaata tcgggttttg gctagcaaaa gaaagccaaa aaggtccggt
    17041 tgatttaaca aataaacgag cgttggattt gatcatccaa aacggaaagt acacaaatta
    17101 tcattttgtt ccagctaaaa cacatattta cgaggttgaa gcgatgtttc gtgagcaagg
    17161 gactttggaa gccgtactta tcactaaaga tgggaatccc gatggtaatc tgctcgggat
    17221 cgtacgacct cgtgatattt ataaacaaat agaaaaggat tgattttttg ttaattattt
    17281 attggatatt cagcgctttg ctagtaggag tggatcagtt ggtcaaatgg tggatcactg
    17341 ataatctagc gcttggagag acgaaaaatc ttataccagg tattttgtca ctgaatcata
    17401 ttcgaaacac tggagccgca tggagtatgc tagaagggaa gatgtggttc tttactatag
    17461 tgacactcat tgcagtcgtt gtgattctca cattgatgat taaaaatcgt gaaaaaggta
    17521 atcgctggtt catgattgga ttgagtttga ttctagctgg tgcgattggc aattttatcg
    17581 accgtattcg tcttggctat gtagtagata tgttccagac agattttatg agctttccga
    17641 tttttaacgt agcagatgtg acacttgtat gtggagtgat ttgtatgtta atttatatta
    17701 ttttagatga aaaggatcaa agaaaaaaat gacagaactt aaaacgacga ttcaccatga
    17761 atctggtagg atcgataaag tattgactaa tttattcacc gattacagtc gttcgcaaat
    17821 tcagctgtgg ctgaaaaatg gagcggtttc tgtcaatgga cagccagtca aagcaaatta
    17881 caaagtaaaa caaggagacg agattactgt cttgatcccc gaaccagaaa cattagatgt
    17941 ggtcccagaa aatatcccgt tgacgatcgt ttacgaagat gaagatgtag cggtgataaa
    18001 caaacctcaa ggaatggtcg ttcatccttc agcaggtcat acgcatggca caatggtcaa
    18061 tgcgctgatg taccatatca aggatctttc tacgatcaac ggcgttattc gtccaggtat
    18121 tgttcaccga atcgacaaag atacttccgg actgctaatg gttgccaaaa atgaccaggc
    18181 acacgaatca ttagctcagc aattaaaaga taagacctct ttgcgcaaat atgttgcttt
    18241 agtccatgga gagattcctc acgaaaaagg aaccatcaat gcgccaatcg gccgttcgaa
    18301 ggaaaaccgg aaaatgcagg cgataagaga agatgggaag tcggctgtca cacattttac
    18361 tgtattggaa cgtttcgaag gatttactct ggttgaatta aaactagaaa ccggaagaac
    18421 acaccagatc cgtgtgcata tgaagtatat cggttatccg ttagctggag atccagtata
    18481 cggaccgaag aaaactttga aaggcaatgg tcagtttctc catgcgcaaa tgttaggttt
    18541 tacgcacccg actacaaacg aaaagatgat ctttgaagca cctcttccag aagtttttga
    18601 aaaaacttta gaaaaattaa gaaaaaacat tgcattttaa tttagataac ggtatagtaa
    18661 ttaacaacga aagcataaaa cttaatagaa ttcctttaag tttagtcccg tgaggctaag
    18721 aagggtggga gcaaggatac gtatggtgcg ccataagtag attgctcatt gacatagtat
    18781 agctatcagt gtatcagtat aagctcagtc taaacattcc tcctgtcctc ttagggcagg
    18841 agttttttta tgcaaaaaag tagggggaat gaaaatgcaa gcaaaagaag tagtagatca
    18901 ggtaaccatg aagcgagcat tgactcgaat tacttatgag attatcgaga gaaaccatag
    18961 tatccaagat atcgtattgg taggtatcaa aacacgagga atctacattg catcaagaat
    19021 tgccgaacgc ctgaagcagc tagaagatat cgatattcct gtaggagaac tggatatcac
    19081 tttataccga gatgacaaaa aagaaaatcc agaagaaccg gaacttcatt cttctgacat
    19141 cccagtttcc ttagaaggaa aagaagttat tttgattgat gatgttttgt atacaggacg
    19201 aacgattcga gcagcaatgg acgcagtaat ggattttggc agaccaagaa aaatttcatt
    19261 agcagtcctg gtcgatcgtg ggcatcgaga attgccaatc cgtgcagatt atgttgggaa
    19321 gaacattcca acatctcggg cggaagagat tttagtagag atgcaagagc ttgatggaca
    19381 agatcgcatc atgattttga aagaggaaga ttagaggaga gagaaacatg tcggagaaaa
    19441 aaggatttca taatgatgac gtggtgttag atatccacga tcgcccaacc ccaggacatt
    19501 gggtaggatt aagtttacaa cacttgttca cgatgttcgg agcaaccgtg ttagtcccca
    19561 tcttagtagg tatcgatcca ggtattgcgc tagtcagttc aggactaggt acattggtgt
    19621 acttatttac tactaaagga aagatacctg cgtacttagg aagcagcttc gctttcattg
    19681 cagcgatgca gcttttgatg aaaacagatg gttatccagc gattgcacaa ggagcagtca
    19741 caactggatt agtctatttg attgttgcgc tgatcatcaa aaagatcggt tcagcatggc
    19801 tggataaaat cttgcctcca atcgtcgtcg gtccagtcat catggttatc gggttgggac
    19861 ttgcggcaaa tgcagcaaca aatgctatgt ataatcaggg cacgtatgat ttcaagtata
    19921 ttgctgtagc gctgatcaca ttaggattaa caattttctt caacatgtgg ctaagaggtt
    19981 ttctcggatt gattccgatt ttactaggta ttattagcgg atacatcgta gccttgctgt
    20041 ttggaatcgt ggatactaag ccaatcgtag acgctgcctg gtttgctgtt cctaactttg
    20101 agattccctt tgtacaatat gaaccaaagc tatatcttgg agcaatcaca acaatggcac
    20161 ccatcgcttt tgtgacgatg acagagcata tcggccatct tatggtattg aataaactaa
    20221 ctaaacgtaa tttctttgaa gaaccgggtc ttcataagac gttgacaggt gatggattag
    20281 cacaaatcgt ggcagccttt gtcggaggtc ctcctgtaac aagttacggt gaaaacattg
    20341 gtgttctggc tatcacacgt gtccatagcg tgtttgtgat cggtggagct gcagtatttg
    20401 cagtcatttt aggattcgta ggaaaattaa gcgctttgat tttaagtatc cctggaccag
    20461 tcatctcagg aatcagtttt atcttatttg gagtaattgc cgcaagtgga atgaagattt
    20521 taatcgagaa caaaatcgat tttgataaaa agaaaaatct gttgattgct tcagttatct
    20581 tagtagtagg aattggcgga ctcgtctttg aagtaggcac atttacattg tcggcaatgg
    20641 cattagcaac tgtacttggc atcattttaa atcttatctt accagaaact tcacgaagcg
    20701 aagaaaacta aggaggaaac attatgatca tcaaatcaga acgtatcagc ttaaagcatt
    20761 tattgaccgt tgaggcactt actgatcaag aagttatggg cttgatccgc agagggcagg
    20821 aattcaagca aggtgctaat tggactccgc aaaagcgcca gtactttgct acaaacctct
    20881 ttttcgaaaa tagtactcgc acacataaaa gcttcgatgt agcggagaaa aaactagggc
    20941 tagaagtgat tgaatttgaa gcaagcacaa gctctgtcca aaaaggggaa acactatacg
    21001 atacagtact gacgatgtca gcattaggcg tagacgtagc ggttatccgt catggtgatg
    21061 agaactatta tgacgaactg atacaaagta aaacaatcca gtgttcgatt atcaatggag
    21121 gagacggaag cggacagcat ccaacccaat gcttgcttga tttgatgacc atttatgaag
    21181 aatttggtca ttttgaaggt ctgaaagttg ccatcgtagg tgatatcact cactctcgcg
    21241 tagctaaatc caatatgcag atgttaaaac gtctaggcgc acaagtattc ttctcaggtc
    21301 ctagagaatg gtacgacgat gaattcgaag tgtacggaca ttatatgcca ttggatgaac
    21361 tactagatca agttgatgtc atgatgatgc ttcgggtgca acacgaacgc catgatggaa
    21421 aagaaagttt ctcaaaagaa ggctaccatc aggaacatgg gttaacagtt gagcgtgcga
    21481 agaagttgca aaaacatgca atcattatgc acccagcacc agttaaccgt gatgtagaat
    21541 tagctgattc attagtggaa ggattacagt cgcggatcgt tcagcaaatg agcaatggag
    21601 tattcgtcag aatggcaatc ttagaagcag tgctaaatgg aaaagcgtaa ttataggagg
    21661 atgaaaaaat gaaaacactc attaaaaacg gtcagatcaa tacacgaaaa aatatgacga
    21721 caccagcaga aatttggata gaagatgggc gaatcaaagc aatcggtaca ggattctcag
    21781 aagccgaatt tgatgaagtg tttgatgcaa agggacaatt gatcacacct ggtttagtgg
    21841 acgtccatgt tcatttgaga gagccaggat ttacatacaa ggagacgatc gaagctggga
    21901 ctcgctcagc tgctcgtggc ggtttcacta ctgtatgcgc gatgccgaat ttaaatcctg
    21961 tacctgatac ggcagaaaag ctgagacaag tatatgacat cattagaaaa gatgcagttg
    22021 tgaaggtatt gcagtatgcg ccaatcacag aaaatctgcg aagtgagaaa ttagttgatc
    22081 aagaagcact gattgaagaa ggtgcttttg ctttcacaaa tgacggtgta ggtgtccaaa
    22141 cagctgggac gatgtatctg gctatgaaag aagctgcaaa aaacaataaa gccctagtag
    22201 cccatacaga agatgaatct ttattatttg gcggcgttat gcacgctggc aagaaggcag
    22261 aagaacttgg tcttcccggg atcttaagcg taaccgagtc ttctcaaatt gctagagatc
    22321 tgttgctagc tgaagcaact ggtgttcatt accatgtttg ccatgtttct actaaagaaa
    22381 gtgtccgtgt aatcagagac gcaaaaaaag caggaatcca cgtaacagca gaagtttcac
    22441 cccatcatct tattttgatc gacgaagata ttcccgaaga tttcggcttt tggaaaatga
    22501 acccgccgct aagaggacgg gaggacagag aagctttgat cgaaggcttg cttgacggta
    22561 cgatcgattg catcgctaca gaccatgccc cccatggatt agaagaaaaa agccaaagtt
    22621 tcatgaaatc accatttggt atcgtaggaa gcgaaacagc gtttcaactg atctatactc
    22681 attttgtaga aactggtcga ttcacgttgg aacaagtgat caattggttg gcagttaaac
    22741 cggctgagat ttttggtttg aatgcaggaa cgctaacggt tggtgccccc gctgatgtag
    22801 cagtttttga tattacacaa acttgcacga tcgataaaga agacttttta tcaaaagggg
    22861 agaatacacc ttttatcggc tggaaagtaa aaggcgaaac acaaatgact tttgtcaacg
    22921 gaaaacttgt gtggcagaag ggagaataaa agtatggaac gtttgcttat attagaagat
    22981 ggaacagttt tcgaaggaaa ggcttttggt gcagatgcta gtgtcttcgg agaaatcgtt
    23041 tttacaacaa gtatgactgg ttatcaagaa actatcactg atcaaagttt taacggtcaa
    23101 atcattacat ttacctatcc tatggtgggt aattatgggg taaatagaga tgattatgaa
    23161 tcgatttctc caacatgcaa aggagtagta gtaaaagaac atgcacgtgt agcttcaaac
    23221 tggcgaaacc aaatgacgct agattcattt ttgaaacaaa aaggaattcc tggaatatca
    23281 ggtatcgata cgcgcgccct tactcgcaaa atccgtgaag tcggaacgat gaaagctagt
    23341 atagttgatg ctggagattc cttcgaacat gcatttgatc agctgaaagc aaccgttcta
    23401 gcaaccaatc aagtgcagca agtctcaact aacaaacctt atccaagtcc aggaacaggt
    23461 cggaaagtca ttgtcattga cttcggcttg aaacatagta ttttacgaga attgagtaaa
    23521 agaaactgta acctcacagt acttccctat aacacagatg ccgagacgat tttagcgcta
    23581 tcaccagacg gagtcatgct gacgaatgga cctggtgatc caaaagatgt gccagaagca
    23641 atcgaaatga tcaaaaaaat ccaaggaaaa gtaccgattt ttggtatttg tctcggtcac
    23701 cagttatttg ctttagccaa cggtgcagat acctataaaa tgaaattcgg acatcgagga
    23761 ttaaatcacc cggtacgaga agtagcgacc ggaagaattg attttacttc tcaaaatcat
    23821 ggctatgcag tcgatgaagc cacgattgat ccagaaaaac tcatcgtaac tcatgtagaa
    23881 gtaaatgacg gaacagtaga aggagtcaaa cacagagatt atcccgcttt cagtgtgcag
    23941 tttcatccag atgctgcccc tggtccgcat gatgcactac atttatttga tgaatttatg
    24001 gaaatgatgg acgcaggaaa ggagcagtaa acagatgccg aaacgtacag atatcaagaa
    24061 aattatggtg attggatctg gtccgatcat cattggtcaa gccgcagaat ttgactacgc
    24121 aggaacacaa gcctgccttg ccttaaaaga agaaggatat gaagtggttt tagtaaattc
    24181 taatcctgcg acgatcatga cggataaaga agtggcagat caagtatata tcgaaccgat
    24241 cacacttgaa tttgtatcaa ggattcttcg aaaagagcgt ccagatgctc tccttccaac
    24301 tttaggcgga cagacaggcc tcaatatggc aatggagctg gcagcatcag gtattttaga
    24361 agaattgaac atagaacttc taggaacaaa gctttctgcg atcgatcaag cagaagatcg
    24421 tgatctattc aaacagttga tgaaagaatt gaatcagcca atccccgagt ctgaaatcgt
    24481 caatacagta gaagaagctg tagattttgc aaataaaatc ggctatcctg tcatcgttcg
    24541 tccagccttt actttaggcg gaactggtgg aggtatgtgt gaaaatgaag aagaattacg
    24601 tatcgtatca gaaaatggat taagtctttc ccccgctacc caatgtttga ttgaacgaag
    24661 tattgcaggt tttaaagaaa ttgaatatga agtgatgaga gattcggctg ataatgcaat
    24721 cgttgtctgc aatatggaaa attttgaccc ggtgggtatc catacaggtg attctatcgt
    24781 atttgcccct agtcaaacat tgtctgacca tgaatatcaa atgcttcgtg atgcttctct
    24841 ttcgattatt cgagcactta agatcgaggg aggctgtaat gtccaactcg ctcttgatcc
    24901 acatagtttt aattattatg tgattgaagt caatccgcgt gtttctcgtt catctgcgtt
    24961 agcaagtaag gcgactggct atccgatcgc taagctagct gctaaaatag cgcttggatt
    25021 aacattagac gaaatgaaaa atcctgttac aggaacgact tttgcagaat ttgaacctgc
    25081 attagattat gttgtagcta aaattcctcg ctggccgttt gataagtttg aaaacggaga
    25141 acggctttta ggcacgcaga tgaaagcaac tggtgaagtg atggctatcg gtcggaatat
    25201 cgaagagtct ttattgaaag ctgtgcgttc tttagaaatc ggtacacacc atatcgaact
    25261 tcctgagttg gcagacatca cggacgatga cctaatccaa aaagtggttc gagcacaaga
    25321 tgatcgcctg ttttatgtag ctgaagctat tagaagaggg tacaccatag aagaattagc
    25381 ggatttgaca aaaatcgatt tattcttttt agataaactg ttgcatatcg tagaaatcga
    25441 acagcagttg aaacagcagc cgttggatga caagatattg aaaacagcga aacaaaacgg
    25501 ttttacagac aaaaaagtgg cggagctgtg ggaaataaac gaagaagccg ttcgccagat
    25561 gagaaaagaa aatgatttga ttcctgtata taagatggtt gacacttgtg cagcggaatt
    25621 cgaatcaaaa acaccttatt tttatagttc ctatgaatat gaaaatgaaa gttcacgatc
    25681 agaaaagccc tcaattctag tactgggatc tggtcctatc cgtatcggac aaggggttga
    25741 atttgactat gcgactgtgc attctgtcaa agcaatccag caggcaggat acgaagcaat
    25801 catcatgaac agcaatcctg aaacagtttc gactgacttt tctatttcgg ataaattata
    25861 ttttgagcca ttgaccttcg aagatgtgat gaatgtgatc gagctagaac agccgatagg
    25921 agtaatcgtt caatttggtg gtcagacagc aatcaactta gccgaacctt tgactgctgc
    25981 tggtgtaaaa atcttaggga caacgatcga agacttggat cgtgcggaaa atcgtgattt
    26041 attcgagcaa gcgctgaaag agttggatat tcctcaacct ttaggggata ctgctacaag
    26101 taaagaagaa gccgtggtta tcgcttcgag aattggttat ccagttcttg ttcgtccaag
    26161 ctatgtatta ggcggacgtg cgatggaaat cgttgaaaac cagcgagatt tagaagatta
    26221 catggaacat gctgtgaaag catctccaga acatccagta ctagtggacc gttacttgct
    26281 tggtggtgaa tgcgaagtgg atgcgatttg tgacggtgaa acagtactca tcccagggat
    26341 catggagcat attgagcgtg ctggtgtcca ttcaggggat tcaatggctg tgtatccacc
    26401 tcaaacatta tctgaatcga tcaagaaaac gatcgaagat tacacgatcc gcttagcatt
    26461 aggtttgaat tgtgtcggga tgatgaatat ccagtttgtc atccatgaaa atgaggttta
    26521 tgtgatcgaa gtcaatcctc gagcaagtcg gactgttcca ttcttaagta aggtgacagg
    26581 aattccaatg gcacaaattg cgacgaaagc tattttggga gagaaactaa agacacttgg
    26641 ttatgaaaac ggactttatc cagaaacaaa agaagttcat gttaaagcac ctgtcttttc
    26701 ttttacgaaa ttgcagaaag ttgatactta cttagggcct gaaatgaaat cgactggaga
    26761 agtaatggga tctgacagac aattagacaa agctttgtac aaggcattcg aagcttctgg
    26821 attacacttg cctgaatatg gagccgtatt atttacgatt gcggacgaaa caaaagaaga
    26881 agcactagaa ttagctaaac gttttacaga aattggctat agtctgatag gaacgaaaca
    26941 tacagctgct tatttcgaaa aaaatggtct ggtcgttgaa acagtagcga aaatttctga
    27001 ggaagcagca gaaaaaaatg tagtcgactt aataagagat ggtaaaacac aagtggttgt
    27061 caatacgatt gataaagatc ggggaaatgc ttctaaagac ggtttcatca tccgaagaga
    27121 agcagttgaa catggtattc cattgtttac ttctttagac acagcagatg cgatcgttcg
    27181 agtgatggaa tcccgtgctt tttcaactaa ccctatttaa cggcgagcac atgttaagac
    27241 aaaaagacga aaaaccgttg caggagggga cagatgaaac aagaaatgat ggaaatcgtt
    27301 cagcaaaaac agctggcacc aaagattttt gaaatgacat tgaaaggtga tctagtaaaa
    27361 gaaatgcagc aaccaggaca gttcttgcat attctcgtgc cgcgtgcaga cttgctatta
    27421 cgccgaccga ttagcttgaa tcatatcgac cacgaaacaa atacttgccg tatcatttat
    27481 cgggtggaag gtgaggggac tagagtcttc tctactcttt ctgccggaga ccatttagat
    27541 gtcatgggtc ctttaggcaa tggttttgat ttgtccaatc ttcatacagg agacgaagta
    27601 tttataatcg gtggaggaat cggtgtccct cctctatatg agttatcaaa acagttagtc
    27661 gaaaaaggaa ttaagccaac gcatttttta ggttttgcca cacatgaagt cgtttattat
    27721 gaaaaagaat ttaatcgtct aggagaaaca agaatcgcta ctgacgatgg aacatacggt
    27781 atgcacggaa atgtcggtaa tttattgtta ggaacgaaga cggaacctac agctgttttt
    27841 gcatgtggtt caaacgggtt attgaagaca gtggaacagc tgttttcttc gcatcaaaat
    27901 gtccagttat cattagaatc ccgcatggca tgtgggatag gggcttgcta tgcttgcgtt
    27961 tgtcatacgc cggaagatga aacaggaaca aaaagtgtga aagtttgtga cgaaggaccg
    28021 atttttaaaa taggagaggt aattatatga atgagaatcc tttagcggtc aaacttcctg
    28081 gcctagaatt aaaaaatcct atcatgccgg caagcggctg tttcggtttt ggcaaagaat
    28141 acggaaaata ctatgatttg aatcagcttg gatcgatcat ggtcaaggca acgactccaa
    28201 atgcccgctt tggcaatccc acgccaagag tggcggaaac accatctggc atgttgaatg
    28261 caattggttt gcaaaatcct ggtctagacg ttgttatgca taaacttctt ccagaattag
    28321 aggagtacaa tgagttaccc atcatcgcaa atgttgctgg tgcttgtgaa gaagactatg
    28381 tagaagtttg tagcaaaatt ggcgaagcac caaatgtgaa agcaatcgag ctgaacattt
    28441 cttgcccaaa tgtcaaacat ggcggaattg cttttggaac agattctgat gttgcttttc
    28501 aattaacaca agcagtaaag aaagtttctt ctgttcctgt ctatgtcaaa ttgtccccaa
    28561 atgtaacaga catcgttcca atcgctcagg caatcgaagc tggaggggca gatgggttta
    28621 cgatgatcaa tactcttctt ggtatgcgga ttgaccttaa gacaagaaaa ccgattttag
    28681 ccaatcaaac aggtggactc tccggaccag caatcaagcc tgtcgctatt cgtttgattc
    28741 accaagtagc aagtatatcc agccttccaa tcattgggat gggcggcgtg cagacagtgg
    28801 atgatgttct tgagatgttt atggctggag caagtgctgt agctataggg accgcgaatt
    28861 tcaccgatcc gtatatctgt cctaaactaa ttaaagagct ccctgtgcga atgagcgaac
    28921 taggcatcga atcgctggaa agattaatca aagaagtgag agaggaacgt gaacgatgaa
    28981 tcaactagac caacgaccaa ttattgcatt agatttttca actaggcaag aggtagaaga
    29041 ctttcttcga tttttcccga aagaagaaaa attgtttgtg aaaatcggaa tggaattgtt
    29101 ctaccaagaa ggacctgaaa tcgtacgtta tctaaaagag gctggtcata atgtattttt
    29161 agatctgaaa cttcacgata ttccgaatac ggtcgaaaaa gcaatgcggg gattggcaaa
    29221 gcttggtgtt gatctcacct gtgttcatgc cgctggcggt attcgtatga tggaggcggc
    29281 aatgcgagga ttagaagagg ggactccaga aggtggaaaa cgtccgctac ttctagctat
    29341 cactcagctt acttctacaa gtgaagaaga aatgcatgcc gatcaactaa tcgaagtttc
    29401 tttagaaaaa agtgtgatcc attatgctag ctgtgccaaa aaagcaggat tagatggtgt
    29461 ggtttcttct gcttgggaag tagaagcgat caaagaaatg gcaggagacg aatttgtttg
    29521 cttaactccg ggtataagac cagaaggaac agtcgcagga gatcagacaa gagtagtcac
    29581 accttctcag gcaaaaaaga tcggctcaac ttttatcgtt ataggtcgac caatcactca
    29641 gtcaactaat ccttataaag catatcaaac gattcaaaca gaatggagct aacctaaaat
    29701 gaatgttgaa caaagtatcg caaaagactt attagaaatt gaagcagtat ttttaaatcc
    29761 aagtaatcct tttacatggg caagtggaat caaaagccca atttactgtg ataaccgaat
    29821 cacgatgagc tatccaaaag tccgaaaaga gatcgcaaaa ggattagcaa gcaaaatcaa
    29881 agaagcttac cctgaagtac aagtgattgc tggaactgca acagcaggaa tccctcatgc
    29941 cgcatgggta gcggaaatac tggatttgcc aatggtctat attcgaagta aggcgaaaga
    30001 tcatgggaaa ggtaaccaaa tcgaaggacg tatatttgaa ggtcagaaga tggttgtgat
    30061 cgaggatttg atttctacag gcggaagtgt cttggaagca gctgaagcag caaaaagaga
    30121 aggtgcggat atccttggtg ttgcggctat ctttacgtat gagttgccaa aaggaaaagc
    30181 aaatttcgaa aaagccgaaa tccctcttat gacgttgacc aattattctg tattgattga
    30241 agcagcttta gaagatcgtt atatcgatga gcaagaatta actctgctaa aagaatggaa
    30301 aaaagatcct gaaaattggc agatttaagc gcgcttgatg aagatagaga aatccgattt
    30361 gtgtctccag aatcatcaat tggaatagca aaaatccaaa aactgattgc gagaagcatc
    30421 ttttcatcag gtatgaaaag aaacttctat cgtgatcagt ttttgatttt tttaaaattc
    30481 atttttctaa gactggttag cagaaagaag agaataatct cttctctttt ttctattata
    30541 tatagatttt ctctattttt tggtaaggga gctgtttcaa aaatggtaaa gagcggaaat
    30601 gttgcgaata tcagttattt aatgatatga tattcggaaa aagaaatctc aaagggaggt
    30661 aacttttatg gggttgaatg aatttatttt aggattgaac aatttagaga cgatcacaag
    30721 ggcacctgga ttttttaaat atacagagca tacagttgca gcacattcgt atcgtgtagc
    30781 atctattgcg caagttctag gagacattga agaagcgaat ggacaaccga tcgattggaa
    30841 actgctctat gaaaaatcat tgaatcatga ctacacagag cgttttattg gtgatatcaa
    30901 aacgccggtg aaatacgcaa gcaaagagtt aagagggatg ctgcaaacag ttgaagaaaa
    30961 gatgacggac gaattcatca accaagagat tccagctgag ttccaagaag tttatcgccg
    31021 tcggttgttc gaaggaaaag atgaaaccgt agaaggagaa ctattagcca tttcagataa
    31081 ggttgacctt ctttatgaat cttttgatga aatcatcaaa aataacccgg aaaaagttta
    31141 taaggagatg ttcattgagg cagtgactac gattaaaagc tttgaacatc ggccttctgt
    31201 tcagtatttc tttaaagaga tatttccgga actattgaac caggaatttt atggaaaaga
    31261 ggcgttttta gaaaccgtct caacgattgt aaagaaagaa gaaaaataaa aatgatatga
    31321 gatgcagggg gaagtatagt tgaaaaagaa tatggatgtc gaatggggct attctggaga
    31381 caaaggtccg gaaaattggg cgagtttatg tgattggttt gcgagaggtg cggaatttcc
    31441 attgcagtcg cctattcatt tgaccaaggg ggaagaaacc aaaatagaag gagacacgct
    31501 ttcctttcat tatcaaaagc aacggtttac tgacaaggaa ttcaaaaaca cgattcattt
    31561 agtacctttt gaccaactca gttatgtaga gttcaagaaa gaacgctatt atttgacaga
    31621 tattcatttc cacttgcctt ctgaacatat catcaacgga aaacaggaag atattgaatt
    31681 tcattttgtt catatgaaca gcaaaaaaga gaatctagtt gtgggtgtca tgtaccagct
    31741 aatggtacag gaaggttggc tgtataatca agaaaatggg gaaagttgga acttagaaaa
    31801 acatgagcat tgggtaaatc cggatgtgtt ctttccggag caaaaaagcc atttccatta
    31861 tattggctca ttgacaactc cgcctaccag cgggccaatc caatggtttg taatggatca
    31921 tgtaggaaaa ctgaaccaag agtttcttct ccccttcgat ggtcagtatg ctcgaccgaa
    31981 caaccgacca atccagccct tgaatggaag agaaatttat tatttcgagg agtttgagca
    32041 atgaatattc agcagatgaa atacgtggtc gctgtagcaa acaatggtag tttcagagaa
    32101 gcggcaaaga aaatgtttgt ctcccagcca agtctttcag ctggtatcaa agaattagaa
    32161 accgagttag ggatcacgct gtttactcga acgaatcgag gggcatattt aacagaagaa
    32221 gggcaagact tcttaaatcg tgccgagaaa atattggtcc agcttgatac attagaaact
    32281 cactatctaa cgagtgaaaa gagagacaat ttttcgatcg ctgcccagca ttatgatttc
    32341 ttaggtcctt tgaccgcaca gctactcgag gaattcaaag acgagatcaa acaatttcgg
    32401 atcatggaga cgacaacagc gagagtgatc gaagaagtca aagagcagca cagtgagatc
    32461 ggcattattt atatgaatga gcataatcaa gctggtatca gaagatattt acaacaagga
    32521 gagctaagtg gccgcagcat tggaaaattc aaaacgcata tttttttaag gcaaggacat
    32581 ccattagctg aacacgcaga gatcaccaaa gaagaattgt tatcttatcc tcaagttcgt
    32641 tttacacagg atggaaataa tttcccgtat ttttatgaag accttatcga gatccctgat
    32701 caagaaacag tgatttatac gagtgatcga ggtactttga tgaatatcgt tcttgaaact
    32761 gatgcttacg cttcaggatc tggtattgtg attggaggga ttcgcgagca cttgcggtta
    32821 atccctttgg cagaaggcga actaaatgaa ttttatatca tttattcggc gaaacgtccg
    32881 ctcagtgaaa tcgctcaaag atttatcaaa gagcttactc ggttactgga ccagcagagc
    32941 taagaaaagc agatgagaac gctgtttatt gaacaccgtt gatcaaaaaa gaggggttgt
    33001 gacagaattt tgtcacaact cctctttttg tagcaaacaa tttataacta attgctgttg
    33061 tgggaaagta cgcaaatatg attctagaaa cactcattga ttacctgttt tgtttcatag
    33121 ctgtaatttt ttcttcttct ggtgtcacga taatcgtctt ttggttttca taaataacat
    33181 aaccaggctt ggcgccattt ggttttcgga tatgctttac ctgtacaaga tcaaccggca
    33241 cttgggcaga gtatcggtat tttgaaaaat aggcagctaa ctcggcagct tctgtaattg
    33301 tttcatctga gggcttgtca cttttaatga tgacgtgcga accgggtata ttttttgcat
    33361 gaagccaata gtcggttttt ttagctgtct tcattgtcag ctggtcattt tgaagattgt
    33421 ttttaccaac aagaatgagc gttccgtcag aagaaaagta ttgatcaggc tgagatggtt
    33481 tcttacgttt ttgttttttt aagttcttct ttttcaggta tccttctgaa gtcaattctt
    33541 ctttaattgc ttcgatatcc attgggccgg caatttccag ttgagataag acagattcaa
    33601 gatattggat ttcatctttg gcttcttgga tttgttcgcc aatcagctta acagcgttct
    33661 tcagcttttg gtatcgatgg aaatatttct gtgcattttg atttggtgtc aatgccggat
    33721 ctagtgctat tttgattggc cgatcttctt cataataatt aggtaaaaca acttcttttg
    33781 cgccgcgtgg tacttgtgtc agaaaagtag taagtagttc gccatttcta cggtaattct
    33841 cagcgttttc ggattctttg agtgtttgtt cacgtttttt caatttattt ttatttcgtt
    33901 ttagttcatt ttcgattttt cgaatcagtt ctccgccttg ttgttttgcc cgatctttct
    33961 ctgctttttc gtgataatag ctatctaata attgactgag tgttggataa gctgttacag
    34021 cagatgcctg ttcggacaat acctgataag caatcggtgt gaaaaaatct ttattttccg
    34081 tttcgtaaaa agtaggaata ggctgatggc tgatggctga gaaaaattcg tcccatacaa
    34141 ccatcttttc attaggacgt tccagtaatc ttttggtcag ctcttgagcg atatcgaaac
    34201 cgattccctg aaactgacgt tggattgctt ttggatctag ttcagtttgg gaaagccgct
    34261 gaaagatttt ttctttctcg gagctaaatg ggttcaagac ctcttgtttt ggcggagcaa
    34321 catattctac accaggaagc aaagaacgat aagtgttttg agagcttcca atgtgtttga
    34381 tcgcatccag tatttttcca gtttctcgat taactaagac gatcgtacta tgacgtccca
    34441 ttaattcaac gatcaatatg atattttgta gatcacccaa ctcgtttcgt ttagcaaagt
    34501 ggaaatggat cactcgatca ttttcgattt gttcgatgtc ttctaaaata gcgccatcca
    34561 aatattttcg aagcatcatc acgaagttag gcggattatc aggattctga taatcgattt
    34621 gggtaatctg tacacgagca taacttgggt gcgcagataa taataatcga tggtttttgc
    34681 cgcgtgaacg aatcaccagc acgatttcat tttcataagg ctgatggatt ttcgagatcc
    34741 ggccagatag taacgtttca cgcaattcgt tgaccatcgc gtgggtaaat acaccatcaa
    34801 aggacatggt tcattcctca tttcttttca taagtatatt cattataacg aaaaaaagaa
    34861 gaaaaaccaa tttggccgaa tagtaaagca aaagtctaga aaaatcttgg actaattaca
    34921 taaaaaacag tgtttctatc gttgaaatcc aaatttttag gtataataaa aaataggaga
    34981 aaaagtagaa aagaaggtgt ttgttatttg ggaagtagtt aaatcgagag tacaagaaat
    35041 gaatgaacat ttctcagtgt cattcgcaga aaatccaagt aagtattttc gacgtcttgt
    35101 ttttctgctc atcttatcat tgctgttaac agttgcaatg tttttattgc cggaaaaaat
    35161 ccgagaagag caagtaaata gtctcgttta ttcgtccata ccaaccagca aattgaaacc
    35221 ttttgattac agcaaagctg atcagaaaat caaacaaagc cgggcgatat cggtgatgtt
    35281 cagcaaaccg aacgggaaaa cctatcagga catgcttgat gtttttaatg atcctgagct
    35341 tatggaagaa ttgaatcgac cgatctttta ttatccgatc gtttatgatg tacatgaact
    35401 ggaacaaaaa tataacatac gaacggatga agtgacattt attttctttg atggaggaaa
    35461 agaagcaaat cggataactg cgggtaaggg aggaataact gattttgata aggagttgat
    35521 ccctgaactg aatcgacttc cgttagccaa tatcaaacaa ttagaagaag aattggatag
    35581 aacgggagta acttcccaaa attccgagat tgctaattga gacattggcg cgatgaaacg
    35641 gtgggtctat atagcgctat ttttaattat cagaacaagt gatgtttgat cttcaaaata
    35701 acaaataaaa aattaaaaaa tatagttgac gaaattcaaa aattcgatta gacttaaatc
    35761 aattcttaag gaaagggtga cctgtaatga acttatcaaa acgtatttca gcagaaaaca
    35821 aaaaaaattg gcgcagctgg cgtagataag ttgtattaat ggtgagactt tcccatagat
    35881 acaacttttt agagtgcatt tctaaagctt gtatctatgc tagacatgaa cattgactgc
    35941 cgcatagatt agaatctatg cggttttttg tgtaaagaca atgaccgctt tggccattgt
    36001 ctttttttgt agactggata cttgcctgat tataataaat aaaaagattg actttgcagg
    36061 aggaaaaaaa tgaaaaacaa acgattaata acagttgtag taattatttt agtttattta
    36121 gcaggtgcat ttatttatgg aaaagcctca ggcgaaaaag aaagtacttc gacgaaccag
    36181 acaaaaaaaa caacagttgt tggggtattg caatacgtga gccatccggc attagatgag
    36241 atttatcgag gaatcaaaga tggtctgaaa gaagaaggat taaaagaagg agaaaatctt
    36301 gagatcaaat ttcaaaatgg acaagccgat cagagcaagc tagccacaat gagtcaacag
    36361 ctagtacaat ctgatccaga tgccttgatt ggaatcgcaa cacctgccgc tcaatcatta
    36421 gcaaacacga cttcatcgat cccagttgtg ttaggtgctg taacggatcc tgtgggggct
    36481 ggattagtcg cttctttaga gaaacctggt ggtaatgtaa caggtgtttc tgatcagccg
    36541 cctgttactt cacaaatcaa gcttgcttct gatttgttgc caaatgcaaa aaaagtcgga
    36601 atcttgtatg cttcttcaga ggataattca aaataccaag tagcacaagc agaagaagct
    36661 gcaaaaaaag aagggttaga atcagtaaaa tatgcggtgc cttccactaa tgagattgca
    36721 caaacggttc aagtgatgag ccgacaagtc gattttatct atgttccatt agataatacc
    36781 attgccaatg cgatgcaaac agtggtaaaa gaagcaaaca aagcaaatat ccccgtcatt
    36841 ccatctgtgg atacaatggt ggaacaaggt ggactagcaa caatcgggat caatcaatat
    36901 caattagggc ttcaaagcgg taagatggcg gcagaacttg cgagtggaaa agaaaaaccg
    36961 gaaacgacgc ctgtttatac atttgatcaa ggggatacag tgatcaatca atctcaagct
    37021 gatcgcttag gaatcattgt tccccaatcg atgaaggaaa aagccaaaat cattacagat
    37081 gactcgcagc aagaaacatc taaggaggat gacaaatgat cgtatcagct atttcacaag
    37141 gcatgctttg ggcaatatta ggattaggga ttttcatgac ttatcggatc ttagactttc
    37201 ctgatatgac gacagaaggg tccttcccat taggtgctgc tgtctgtgtg acagctatta
    37261 ctcatggggt agcgccgatt gtagctacat tattaggcgt aggtgctggc atgatagcag
    37321 gactcgttac ggggttactt tatacaaaag gaaagatacc ggttatttta gcagggattt
    37381 tagtgatgtc aggattgaac tcagttattt tgtttgtgat gcgaacacca aatctttctt
    37441 tattgaatca tccgattcta caagacagct tccatcgctt tgggttacct gattactttg
    37501 acacagtcct tcttggcttc atctctttgg gcatcttgat tgctgtactg ctattcttct
    37561 tcaatacaga tttgggtcaa ggctatattg cgacaggaga caacgaaacg atggctcggt
    37621 cgttagggat aaaaaccgat cgtatgaaaa ttttaggttt aactttgtcg aacggtgtca
    37681 ttgcattatc cggtgcgctg attgcccaga acgacggata tgcagatgta aataaaggga
    37741 ttggcgtaat cgtcatcggt cttgcttcca tcatcatcgg tgaagtgatc ttccaagaat
    37801 tgacgctagc agaaagattg attgctattg ttgtcggcag tatcatttat cagctattga
    37861 ttttagtagt aatcaaacta gggtttgaca cgacttatct gaagttgttc tccgctatca
    37921 tcttagctat ttgtctgatg atcccgcaat tgaagaaagc actgaactta aaaacgggtt
    37981 ttgagaagga ggcatagtaa tgactgatat attgacaatc aaaaatggca gaaaagtcgt
    38041 aaacaatgga atgaatgaag agaaagtact attggatgaa attaacctta caatcagaga
    38101 aggggacttc atcactgtcc ttggcgggaa tggtgctgga aaaagcacac tgttcaatac
    38161 gatcgctggt acgcttcaac tcagtcaagg atcgatctct tttaaagatc agctgatcac
    38221 aaaagattca gaagaaaaaa gagcacgatt cctttctcga gttttccaag accccaagat
    38281 gggaacggct cctcggatga cagtagcaga aaatttgcta cttgcacaaa aaagaggtca
    38341 aaaacggaaa ttccgtttac gtaacttgaa aaatcaaaaa gaagattttt tcagactatg
    38401 tcaagaagtc ggtaatggcc tagagcaaca cttggatact ccagcgggtg aattatctgg
    38461 tggtcaacgc caagcattaa gcttgctgat ggcgacgatc acagctcctg agttactatt
    38521 attagatgaa catactgcag ctttagatcc taaaacagct aaagcattaa tgcgattaac
    38581 aaatcagcgg atcaatgaac aaaaattgac ttgtctcatg atcacgcatc gaatggaaga
    38641 cgccttgaaa tacggaaatc gtctgatcgt actccaaaaa gggaaaatcg ttcaagatat
    38701 ttctgaaaaa gaaaagcaac atttgacaat gggcgacctt ttgcagttct ttgaagaagt
    38761 agtggattga tttcgacaga aaaattcctt tcagtagaag gacttgaaaa ctcattgaca
    38821 tcttcctttg attcatccta tacttaaaac aataaacaat taaacatatt tgggtgtttt
    38881 gtaggatcac gtagtcaatg attttaacaa ttaaatagaa tattctttgc tctgacaagt
    38941 aaacgatgag aaaagaattt gctactttgc cacctagacg tttcgtactt tttcgtatca
    39001 aacgctaagt ggcttttttt attgaagtaa gaacaaagga ggaataagat gatttctgga
    39061 caaaccaaat tagctgggct ctttgctgat ccggcttctc acagtttatc cccattgatg
    39121 cacaatactg cttttgaagc aaacaacatc gatgcggtgt atttagcttt ccaagtaaat
    39181 ccaatgaatt tgaagcaagc agtcgaaagt attcgaactt ttgatatgtt aggtgtaaat
    39241 ctttcgatgc cgaataaaac agcggttatt ccttatctgg atgaggtaag ccaggaagcg
    39301 caattgatcg gagcagtgaa tacgatcgtg cacgaaaagg gtcgtttgat tggctataat
    39361 actgatggtg cgggcttcat gcaatccgtg caagaagctg ggatttcgat caaaaagaaa
    39421 aaaatgactc tactgggagc tggaggtgca gcaaaggcag tcactgttca agcagcgtta
    39481 gacggcgctg aggaaatcgt agtttataaa cgaaacaacc aaacattcgc tcaagtaaaa
    39541 gaaacattcg aacaactcag ctatcgaact tcttgtaaga taaaagttcg tgactttgcc
    39601 gatacgcatc aattaagaaa agacttgaaa gaaagctgtc ttctagtcaa tgcaacagat
    39661 atcgggatgg gaaagaaaat caatcagtca ccgctcttag atataagcat gatgcatgag
    39721 aatctagcgg tttttgattt gatttatgca cctagagaaa cacgtttatt aaaagaagca
    39781 aaaaaagtag gagcaaaagg atacaatgga cttggcatgt tgatctacca aggagcaatc
    39841 gcgtttgaat tatggacaaa ggaaaagatg ccgatccatg tgattgaaaa gctgtttgat
    39901 taaaaaagga taatagataa agatctttta ttctcgcata aacggtctca agaaaagtaa
    39961 tcctttatgt atcgaagcat ttatttcgtt attttttatt aaattatcaa aaaatgatag
    40021 gcttttgtcg attggttatc gtatccttta tttatcagta agaaacgagg aggaaccaca
    40081 atgagtcgga tggatcgcta taaggacatc cataagaagg cgaaacctct gaagcgggat
    40141 acggggagta tttttgctag aagagaacga aaacgaacgc gtatggaaaa tgaggaagat
    40201 gctacccgta tttatgaaag agagcagcat tcatttgaac atttgaacca atacgagcaa
    40261 cagatataca gagatgcacc aagagcagaa gaacaacaaa aaaataaaaa atttttcaat
    40321 cggcaaaaga agaacaaacc gttaaaacct cgaagaaagc gatcgtggaa aaaaatcatt
    40381 ctatcgattt tgcttgtttt gatcctttat tcaggtatct cattttttat tggtaaatat
    40441 gtagctgaac atgacacaag cttagaggca gcgacttcag aaacctttca tgggcaaaaa
    40501 gcgacgaatg gtgcgcataa tattttgatc cttggaagtg atacacgcgg agaagatgcc
    40561 gggcgagccg atacgatcat ggtcttacag ttggatggtc cttcccataa gccgaaactg
    40621 atttctttta tgcgtgacag cttcgttacg atcccagggg taggacaaaa taagatcaat
    40681 tcagcttatg catacggagg agccgatcta gtgcgtcaaa ctttagtaga aaattttgga
    40741 atcgattgtc aatactatgc gaaagtcgat ttcaaatcgt ttgaaaaagt gatcgatgcc
    40801 ttattcatga acggagtgaa gatcgatgca gaaaaagatc tgaatctgga tggtgtagat
    40861 attaaaaaag ggggccagaa gatggatgga catgttcttt tgcagtacgc acgtttccga
    40921 atggatgaac aaggagactt tggccgtgtc agacgccaac agcaagtaat gaacgctatt
    40981 ttcagccaat tgaagaatcc attgaatctg atccgtgcac cttatgcagc tggtaaagct
    41041 ttaggatata catccacaga tgtcactgct tggttcttga cgaaaaacct cttttcgatt
    41101 gcacgaggag ctggtggcat tgatcgaata agtgtgccag tagacggttc gtggaattat
    41161 gggcaaagtg attatgctgg cagtattctt gaaattgata atcaaatgaa tcaatctgct
    41221 attacagatt ttttgaataa atgatcataa aaaagatcaa gaagaagctg cgcaaggctt
    41281 ttgtcatgaa tcaatcaaac gattcactcg taaaaaacgc tttcttatgg tagatttaac
    41341 caagagagga gctgtttaca ttggatatct atctgaactt caaaaatgag tcgcgtgaag
    41401 ccatcgcttt ttatgaaaaa gtttttcaga ctgaagcatc aggtatcacc acatacggtg
    41461 aaatgcctgg aatagaacaa atgaacttgc cagaagaagc aaaaaatcta atcacgaatg
    41521 cacggctgaa catttatgat acattagtta tgtttgcaga tacgccagaa ggaattggtc
    41581 ctgaattagt tgtgggaaat aatgtttctt tagtcatcca gacaagaaat gaagaagaaa
    41641 cgaagcgcct atttgaaagc ttagctgagg gtggtaagat tgagatgcca ctggatgctg
    41701 ttccgtgggc tcagttgtat gggatggtaa ctgatcgatt tggcatcaac tggcagatta
    41761 accaagagtg acagctaaag atgaatattt tctggaaaca tcaagcagca aacggatcat
    41821 cgtttgtttg cttgatgttt ttgtgtctat ttcgactata cggtatcttt tttttattgc
    41881 cagatgcgaa aatatgttcc ataatagaga agtccgcaaa aaattaagta acggaggtca
    41941 gaagatgaag acggtgatac tagctgaaaa accttcacaa gcaaaagcct atgccgaaac
    42001 cttccaacag caaaagcggc atgaagggta ttatgaaatc agcgatccat tgtttgctgg
    42061 tgaagtaacc atcacttatg gttttggaca tttggtagat atggttgctc caggagctta
    42121 tgaagagcgt tgggcaaaat ggtctttaga aaatttgcct attttcccag aaaccttccg
    42181 ttacgaagtt ccaatggaca aacgtgcaca attttcgatc gtaaaacgag aattgcagtc
    42241 agccgatacc atcattattg caacagatgg tgatcgagaa ggtgaagcca tcgcgtggtc
    42301 gattattcga caagcaaacg cattttctaa ggaaaagaac tatttacgtt tatggatcaa
    42361 ttctcttgaa aaagaagcaa tctatgaagg ttttaagaat ctccaacctg gaaagaacta
    42421 ttttccaaaa tataaagaag cgcaagcaag gcagaacgct gattggctga ttggaatgaa
    42481 tggaagtcca ttatatagct tgctacttca acgaaaaggg attgatggca gtttttctct
    42541 tggtcgtgta caaacaccga cactctatat gatttatcag ctgcaagaag aaatcaaaca
    42601 cttcaaaaaa gaaacgtact ttgaaggaga aggtttaatt acgacaaaca aagggcagtt
    42661 ttctggaaaa atcgtaccaa aaaaagcatt taaaacccaa gaagagttaa cgaaggaaat
    42721 agaaaaactt ggagcgcatt taggtaagca aaacggacgg attcttgaag ttacgaaaaa
    42781 ggaaaagagg ttgcatagtc cacgtttatt ttcgctatct agtttgcaga caaagatgaa
    42841 tcagctaatg aaagcaagtg ccaaagctac acttgatgct gctcaaggat tgtacgaaag
    42901 caagtttcta agctatccaa gaacggattc cttttacatt acagaaaatg agcatcagta
    42961 tctggttaaa catctgcaaa attataaaaa atttttagga atagaagaag tacagacaac
    43021 tgagatcaaa gcaaagaaac gatatgtcga tgcgaaaaaa gttcaagaac atcatgcaat
    43081 tattccgaca aagacgattc cgactcctgc acgctttgcg aagctttcca aattacagca
    43141 agcaatctat ttgcaagttt tgcgcactac tgtggctatg tttgctgcag attatatcta
    43201 cgaagagacg accgtaatga cgggcgtaca gcaattaaag ctaaaaagca tcggaaagat
    43261 cccattacag caaggttggc aggaaatctt acgcatcaag tcaaaaaaga aagaagctca
    43321 gactcttcct cctatcgtag aaaatgaaga agcagaagta gaactgaaaa gtatggagaa
    43381 agaaacacaa cctccaaaac cctataccga aggaacgttg atcacagcaa tgaaaactgc
    43441 gggaaaaacg ctggataatg atgaagcgca agaaattctc aaagaagtag aaggaatagg
    43501 gacagaagct acacgagcca atatcattga aaatcttaag cagaagcatt acatcgaagt
    43561 caataaaaac gaaataactg ttacgccaaa aggaatcact ctttgtaagg cagtagccaa
    43621 tgaaccatta ctgacgagcg cagaaatgac tgcgcgctgg gaagggtact tgagaaaaat
    43681 tggtaaacaa gaggggacac catctgtttt tttagagaat atcaaaaaat tcatcctaca
    43741 tttattggca gaagttccta atcaaatcga caaggtagac ttttcagatg agattagcgg
    43801 gacacgtcag ataaaagcga tggagaagaa aaacgatcaa ttaggaagat gtccaaaatg
    43861 taaaaaggga atagtcatgc tttatcctaa aatcgctaca tgcttgaatc cggaatgcga
    43921 cttcaaattg tggccaacag tagctaaaaa gaagctgact aaaacgaatc tcagagattt
    43981 gctttcaaaa ggaaagacaa gtaaagttgt caaaggtttt actggtaaaa aagggaaatt
    44041 cgatgcagtt ttagtattaa aagaagatat gacaatcggt ttttcttttc catggatcga
    44101 aaacagtgga acaaaagcag ttgatggaaa caaagaaaca tcaagaaaaa ataatgaaaa
    44161 cgaagtagag taaatgaaaa caatgaaata gcggaatatt tattccgaaa agtgagaata
    44221 taaataccaa taaaactaaa aaatatcagt gaatcaagca aaaacgttgg ggtaggctga
    44281 ttttatatag atagcaagga gtttttttat ttgaaaaaag tgcattcatt tttaaattat
    44341 cttatgggag caatcacgat tgtggctgtg taccaagcat ttctaaagta tcctgattac
    44401 gggacgaagt tgttgtcatt gacgcacaat caaacagcat attcaccaac gttgatttta
    44461 tcgactttga ctgcaggagc taaaagcgct acttattttt ttcataaaac gacaagcatg
    44521 ctaaagcgaa agaaacagcg aaaacaattg ttggtggtgg tagcggctat gatcattatt
    44581 tattttctac cagttattac acaagtattt catcaatatt ttggttaaga atacgagagt
    44641 ttgtgacaaa agtcatgtca caaacttttt tctttgtcat tttttcgtaa gaaaaagcaa
    44701 aataaaagta tatacattga ttaaaagtat atacttggat ataatgtact tactatagaa
    44761 tgagaatggt atagagggtg gcgaaatatg aaaaaaatga aaaagtataa aattagaaaa
    44821 tctggcaata gcgatgtaac aactatccca ccagaagtaa aagaattcat gggtgtgcag
    44881 acaggtgacg caatcagtta tgtttttcaa tctgatggtt ctgtacgaat gatcaaagca
    44941 caagaagaac ctgatattga ttcattagtt gattcgatca tgaatcaata tgaagacgcg
    45001 ttaaaagatt tggttgattt atgaggtatc tttcgactaa agaaattatc aaaatcaacg
    45061 cgaaagttat tttaccgtca ctcgccaggc gaaatgatcg gtgtaaaaga tgcaaatgca
    45121 ttagatatgg ccgtcaaaca gccttcacaa gcagtattca atcaagaact ctatcctgaa
    45181 atctatgaaa aagcggcgat tttagcaatt aatttagcta aaaaacatcc ttttcataat
    45241 ggcaataaac gaactgctct tgttgccatg ttgttgtttc ttcaattgaa taattgtcca
    45301 gttgcttttt ctcgtcaaga agctgttgat tttatcataa tgattaccac tagttcattg
    45361 gatttcgatt cactaaagtc caagataacc agttatttac gacaaaccgc aataaaatga
    45421 acgaattaaa aacttt
//
LOCUS       DASJUF010000008        44520 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJUF010000008 DASJUF010000000
VERSION     DASJUF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.008"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..1189)
                     /locus_tag="U4B62_000419"
     CDS             complement(38..1189)
                     /locus_tag="U4B62_000419"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN2140603.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="U4B62_000420"
     CDS             complement(1202..1639)
                     /locus_tag="U4B62_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2140604.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="U4B62_000421"
     CDS             complement(1654..3420)
                     /locus_tag="U4B62_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN2140605.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="U4B62_000422"
     CDS             3684..4361
                     /locus_tag="U4B62_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2140606.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="U4B62_000423"
     CDS             4364..5491
                     /locus_tag="U4B62_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140607.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="U4B62_000424"
     CDS             complement(5691..6131)
                     /locus_tag="U4B62_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2140608.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="U4B62_000425"
     CDS             complement(6142..8175)
                     /locus_tag="U4B62_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2140609.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="U4B62_000426"
     CDS             complement(8177..9880)
                     /locus_tag="U4B62_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2140610.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U4B62_000427"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U4B62_000427"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN2140611.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="U4B62_000428"
     CDS             complement(10963..11988)
                     /locus_tag="U4B62_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2140612.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12197..13066)
                     /locus_tag="U4B62_000429"
     CDS             complement(12197..13066)
                     /locus_tag="U4B62_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN2140613.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="U4B62_000430"
     CDS             complement(13525..15417)
                     /locus_tag="U4B62_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2140614.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="U4B62_000431"
     CDS             complement(15414..17141)
                     /locus_tag="U4B62_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2140615.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="U4B62_000432"
     CDS             complement(17157..17807)
                     /locus_tag="U4B62_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2140616.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="U4B62_000433"
     CDS             18011..18307
                     /locus_tag="U4B62_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2140617.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="U4B62_000434"
     CDS             18479..19099
                     /locus_tag="U4B62_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN2140618.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="U4B62_000435"
     CDS             complement(19191..20357)
                     /locus_tag="U4B62_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2140619.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="U4B62_000436"
     CDS             complement(20369..20722)
                     /locus_tag="U4B62_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2140620.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="U4B62_000437"
     CDS             20850..21872
                     /locus_tag="U4B62_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN2140621.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="U4B62_000438"
     CDS             21891..22052
                     /locus_tag="U4B62_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140622.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="U4B62_000439"
     CDS             complement(22311..22745)
                     /locus_tag="U4B62_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140623.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="U4B62_000440"
     CDS             complement(22855..24714)
                     /locus_tag="U4B62_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2140624.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="U4B62_000441"
     CDS             complement(24832..25992)
                     /locus_tag="U4B62_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN2140625.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U4B62_000442"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U4B62_000442"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN2140626.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="U4B62_000443"
     CDS             complement(27739..28437)
                     /locus_tag="U4B62_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2140627.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="U4B62_000444"
     CDS             complement(28394..30055)
                     /locus_tag="U4B62_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN2140628.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="U4B62_000445"
     CDS             30273..31673
                     /locus_tag="U4B62_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN2140629.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="U4B62_000446"
     CDS             complement(31925..33004)
                     /locus_tag="U4B62_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2140630.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="U4B62_000447"
     CDS             complement(33090..34049)
                     /locus_tag="U4B62_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2140631.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="U4B62_000448"
     CDS             complement(34061..35572)
                     /locus_tag="U4B62_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2140632.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="U4B62_000449"
     CDS             complement(35594..36157)
                     /locus_tag="U4B62_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN2140633.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="U4B62_000450"
     CDS             complement(36163..37026)
                     /locus_tag="U4B62_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2140634.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="U4B62_000451"
     CDS             complement(37040..37936)
                     /locus_tag="U4B62_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2140635.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="U4B62_000452"
     CDS             complement(38111..39400)
                     /locus_tag="U4B62_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2140636.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="U4B62_000453"
     CDS             complement(39372..40889)
                     /locus_tag="U4B62_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2140637.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="U4B62_000454"
     CDS             41087..41977
                     /locus_tag="U4B62_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN2140638.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="U4B62_000455"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="U4B62_000455"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN2140639.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="U4B62_000456"
     CDS             complement(42886..44358)
                     /locus_tag="U4B62_000456"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN2140640.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12832 a         9319 c         7720 g        14649 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgcc ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgt
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
//
LOCUS       DASJUF010000009        43737 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJUF010000009 DASJUF010000000
VERSION     DASJUF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.009"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..451)
                     /locus_tag="U4B62_000457"
     CDS             complement(107..451)
                     /locus_tag="U4B62_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2140641.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(444..878)
                     /locus_tag="U4B62_000458"
     CDS             complement(444..878)
                     /locus_tag="U4B62_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN2140642.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1016..1753
                     /locus_tag="U4B62_000459"
     CDS             1016..1753
                     /locus_tag="U4B62_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2140643.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1746..2957
                     /locus_tag="U4B62_000460"
     CDS             1746..2957
                     /locus_tag="U4B62_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140644.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3161..3943
                     /locus_tag="U4B62_000461"
     CDS             3161..3943
                     /locus_tag="U4B62_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN2140645.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4013..4675
                     /gene="trmB"
                     /locus_tag="U4B62_000462"
     CDS             4013..4675
                     /gene="trmB"
                     /locus_tag="U4B62_000462"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN2140646.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4773..6527)
                     /gene="ade"
                     /locus_tag="U4B62_000463"
     CDS             complement(4773..6527)
                     /gene="ade"
                     /locus_tag="U4B62_000463"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN2140647.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6659..7588
                     /gene="tadA"
                     /locus_tag="U4B62_000464"
     CDS             6659..7588
                     /gene="tadA"
                     /locus_tag="U4B62_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN2140648.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7545..8594
                     /locus_tag="U4B62_000465"
     CDS             7545..8594
                     /locus_tag="U4B62_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN2140649.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8591..8869
                     /locus_tag="U4B62_000466"
     CDS             8591..8869
                     /locus_tag="U4B62_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN2140650.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8844..9314
                     /locus_tag="U4B62_000467"
     CDS             8844..9314
                     /locus_tag="U4B62_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN2140651.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9331..9633
                     /locus_tag="U4B62_000468"
     CDS             9331..9633
                     /locus_tag="U4B62_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140652.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9650..10021
                     /locus_tag="U4B62_000469"
     CDS             9650..10021
                     /locus_tag="U4B62_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN2140653.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            9993..10352
                     /locus_tag="U4B62_000470"
     CDS             9993..10352
                     /locus_tag="U4B62_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140654.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10524..11531
                     /locus_tag="U4B62_000471"
     CDS             10524..11531
                     /locus_tag="U4B62_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2140655.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11556..12740
                     /locus_tag="U4B62_000472"
     CDS             11556..12740
                     /locus_tag="U4B62_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN2140656.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13177..14760)
                     /locus_tag="U4B62_000473"
     CDS             complement(13177..14760)
                     /locus_tag="U4B62_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2140657.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15338..15481)
                     /locus_tag="U4B62_000474"
     CDS             complement(15338..15481)
                     /locus_tag="U4B62_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN2140658.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15704..16183)
                     /locus_tag="U4B62_000475"
     CDS             complement(15704..16183)
                     /locus_tag="U4B62_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2140659.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16256..16540)
                     /locus_tag="U4B62_000476"
     CDS             complement(16256..16540)
                     /locus_tag="U4B62_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140660.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16618..16812)
                     /gene="ssrS"
                     /locus_tag="U4B62_000477"
     ncRNA           complement(16618..16812)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U4B62_000477"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16884..18170)
                     /locus_tag="U4B62_000478"
     CDS             complement(16884..18170)
                     /locus_tag="U4B62_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN2140661.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18308..18979
                     /locus_tag="U4B62_000479"
     CDS             18308..18979
                     /locus_tag="U4B62_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN2140662.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18945..19433
                     /locus_tag="U4B62_000480"
     CDS             18945..19433
                     /locus_tag="U4B62_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN2140663.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19446..20393
                     /gene="prmA"
                     /locus_tag="U4B62_000481"
     CDS             19446..20393
                     /gene="prmA"
                     /locus_tag="U4B62_000481"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN2140664.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20394..21158
                     /locus_tag="U4B62_000482"
     CDS             20394..21158
                     /locus_tag="U4B62_000482"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN2140665.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21391..23604
                     /locus_tag="U4B62_000483"
     CDS             21391..23604
                     /locus_tag="U4B62_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN2140666.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23615..24061
                     /locus_tag="U4B62_000484"
     CDS             23615..24061
                     /locus_tag="U4B62_000484"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN2140667.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24232..25491
                     /locus_tag="U4B62_000485"
     CDS             24232..25491
                     /locus_tag="U4B62_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN2140668.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25580..26527)
                     /locus_tag="U4B62_000486"
     CDS             complement(25580..26527)
                     /locus_tag="U4B62_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2140669.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26921..28213
                     /locus_tag="U4B62_000487"
     CDS             26921..28213
                     /locus_tag="U4B62_000487"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN2140670.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28228..29997
                     /gene="aspS"
                     /locus_tag="U4B62_000488"
     CDS             28228..29997
                     /gene="aspS"
                     /locus_tag="U4B62_000488"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN2140671.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30106..30981
                     /locus_tag="U4B62_000489"
     CDS             30106..30981
                     /locus_tag="U4B62_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2140672.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31247..33130
                     /locus_tag="U4B62_000490"
     CDS             31247..33130
                     /locus_tag="U4B62_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2140673.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33205..33489)
                     /locus_tag="U4B62_000491"
     CDS             complement(33205..33489)
                     /locus_tag="U4B62_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2140674.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33479..34213)
                     /locus_tag="U4B62_000492"
     CDS             complement(33479..34213)
                     /locus_tag="U4B62_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN2140675.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34342..34965
                     /locus_tag="U4B62_000493"
     CDS             34342..34965
                     /locus_tag="U4B62_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN2140676.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35157..36278
                     /locus_tag="U4B62_000494"
     CDS             35157..36278
                     /locus_tag="U4B62_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN2140677.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36285..39413
                     /locus_tag="U4B62_000495"
     CDS             36285..39413
                     /locus_tag="U4B62_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN2140678.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39432..42626
                     /locus_tag="U4B62_000496"
     CDS             39432..42626
                     /locus_tag="U4B62_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN2140679.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42772..43479)
                     /locus_tag="U4B62_000497"
     CDS             complement(42772..43479)
                     /locus_tag="U4B62_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2140680.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14107 a         7767 c         9237 g        12626 t
ORIGIN      
        1 gttgtcgtgg tagtttcaaa caaaattcaa aatttcatca cattcctagg aaatacgcaa
       61 acttttctca cttatatagt taaatctgtt gagaaaataa tcattattat atttgttcag
      121 gatactctgg cagatcagct tttagttttt ctacttgttc attgatttgt gtgatacgtg
      181 gttcggcttg gaatttaaaa tcttcgatat cttttttgat tgcttcttgg aaagcaggaa
      241 tcacagattc agctgtttct tttgtttcga tcaatgcgct tttaaaatga ttcaggctgt
      301 tatttaaatc atttgtcaga gttgttgctt cttccaattc ttcgatcagt ttctttcttg
      361 tttcattccc gctcctgggc gccaataaca gacctccgac agtgccagca gcagctccaa
      421 ataacagtcc tttgaaaaag tttttaatca tcgcaggaca cctgctttac gattgtttct
      481 tgaatagctt ttagttcttc cgatccataa ctatcttgat gattgacaaa gtgaatagaa
      541 aaatcatcct ctttgctgta gcgagggact aaatggatat gtgaatgaaa aacagattga
      601 taggctagtt cacggttgtt attcaagata ttaagacctt tcatttctgg aaaagctttt
      661 tccaatgctt gagcaatctt aggcacacgc gaaaaaacct ctcctgcaag ttcgggatca
      721 tattcaaaaa tatctgctac atgacgttta ggcacaacta atgtgtgtcc tttcgttgct
      781 tgagagatgt ctaaaaaagc atagaccttg tcgtcttcat atattttata gcttgggatt
      841 tcttgattga tgattttgca aaaaatacag ttttccatat aaaacatcct ttctataaag
      901 aataaatact tcagcttaac tttaccataa gttaaagcta ttgtaaggaa aaaaacaaaa
      961 taccgtcatt ttcttcgtgg tataataaat aaaaataaat ggcggaggaa aaagaatgag
     1021 tttaattatc gatcatgtca caggggggta cggacatctc cctgtgttaa aagatatcag
     1081 tttcgatgta cagcctggag agatagtagg gttgattgga ctgaacggag cagggaaaag
     1141 tacgaccatc aagcacatta tcggtctgct ggaagcttca aaaggaacga tagaaatcaa
     1201 tcatctatcc ttaaagaaaa atacggaaga atatcgcaaa caaattgggt atattccaga
     1261 gactccttct ttatatgaag aattgacatt gagagaacat atcgaagtca ctgcgatggc
     1321 ctacgatata cctatggata tagcaatgaa aagagcagaa aagttgttgg agacttttcg
     1381 gttatctaat cgtcttgact ggttcccagc taatttttca aagggaatga aacagaaagt
     1441 gatgatctta tgcgcctttt tgatcgagcc ttctttatat atcatcgacg aaccattttt
     1501 aggcttggat ccactagcta tcaatgcatt gcttgaattg atggtagaaa tgaaaaaaga
     1561 aggagccggt attttgatgt ctacgcatat tttagctact gcagaaaaat attgtgatcg
     1621 ttttgtggtc ttacataacg gagagatacg agcagacggt tctcttgaga tgttgagaaa
     1681 agaatttcag ctgccggatt cttctttgga cgaaatctat attgctttaa caaaggagca
     1741 ggcacatgag tagttttttt caaaaacgtt tagcaagaca tcaaaagaaa atgatgaaat
     1801 atatgcgtta tgtattgaac gatcattttg ttttagtttg tcttttcttg attggcggtg
     1861 tgggattata ttattctaat tggttgaaaa ccctgcagcc accgtttgca attggagggc
     1921 tcataatctc tgtattttgg atgctctgtt tattcgtggg taagatggct acttttgcag
     1981 aggcagcaga tattgttttt ttacttccta aagaaaaaga aatgcaaacc tatttggaac
     2041 gagcttttcg ctattcttgc atttttccgt tgatcttttt gatattggca tgcggattgg
     2101 ccatgccatt agtggtggtt agtacggagc aatccttttc gatgtttttc gtctatctga
     2161 tcatattatg gagtttgaaa ttcagtcatc tgcaaatcaa gagagcagaa ttgtttcgac
     2221 tagatcaaca agttattcgt tattggaaat taggctggtt tttttcttct ctgatctttt
     2281 tgtttctatc attttattgg ctaatatggg caggaatagc aggtgcatta gtacaggcaa
     2341 gtctatatta ctggtttttg tggaagcaac aagaaaaagg attggattgg gagaaaatgg
     2401 tgcatgtaga agaacatagg ttgcatcaga tctatcaatt tatcaattta ttcacagatg
     2461 ttcctgaaat tacggcaaag atcaaaagaa gacgctattt ggatcctata ttgactcata
     2521 ttaaaagaga atcaaaaaac acttatcttt acttgtttgc aagaagattg gcaagaggtt
     2581 ctgattttag tggtctttat ttccgattag taattatagg tgggttattg attgccggag
     2641 tagtcgactt ttattttagt ctgattatcg gtttcttatt tttatatttg atcggtttcc
     2701 aactgttacc tttagcgaac cagttccgct atatggttct cgttcagctt tatccaatcc
     2761 ataagaatca gaagaagcag gcgattcagt atctactcac atgggtatta attatcgcgt
     2821 cggttatttt tggtttgatt gctgcagtcg tattagatgg gcagcagcga tgggttccgc
     2881 tagcggctta tttgattgtt actatgctct ttgttaaact atatgtacca actcgtttga
     2941 agaagatgtc agactgaaaa caaagctgtg tagggtaaaa atgagaatga aaaatagagc
     3001 gttgacagta agaatgctgt tcgttctatt ttttttgaga aaagctggtt ttttgaatgg
     3061 caattaaatg aaaaagaacg tatatgtgct tgacgtgtag tgttcaggcg actagaataa
     3121 taagtactgc aaaacaaaga gtaagaggac gggagaatgc atgatggagt ttcagttaga
     3181 ccaagagtgg cggttacgtc ccatcaaagg cgacacagga aaaacataca taggcatgcg
     3241 tgatgaggac aaagtcttta ttaaacgaaa cacgactccg atgttggctg ctctttcaag
     3301 ggaaggaatt acaccaaaac ttgtttggac aaaacgaacg gggaatggcg atactttgac
     3361 agcacaagag tggctggaag gtcgcttgct gactcctaat gagattggtc aaagaaacga
     3421 cgtgattgat gttttgtacc atttacatca ttcaaattct cttaaaacca tgctgacccg
     3481 tatcggtggt cgtgtcatga cgccggagaa gatgctgcaa aaatatgaaa aagaattacc
     3541 ggaagaactc cagaaaaatc aatttctgca aattgtccat cgttttcttc gtgagcagat
     3601 cccgtcaata gataaggaaa acttatcagt agtacacgga gatgtaaacc atcgtaattg
     3661 gttagtatgt aaaaattatt tatatctagt cgattgggat tcaatcatgt ttgcagaccc
     3721 tgcggtggat attgcaacga ttttaggaaa ttatgtgcca ctgtctagct ggaatcagtg
     3781 gctgatcagt tatggcatac gcccgactga cgaaatgctt gaaaaatttc attggtatgc
     3841 ggccatgaat ttgctccaag aaatcaccag atattgcaaa cgtgaagatc atcgacggat
     3901 gaacgaagaa attttacaat taaaacgaat tttcagtggc tgaggtttgg cccaaactct
     3961 gcggagattt ggccagacct ttttaattag aggaaaggaa tggagataaa taatgcgtgt
     4021 ccgaaatcgt ccgggagcag cggaaatgtt agcggctcac ccgaattttg taattagtga
     4081 tccaacattg tggaagggaa aatggaatga attgtttgaa aacgatcatc caatccacat
     4141 tgagattggt atggggaaag gccagttcat cactggaatg gcaaaagccc atcctgaaat
     4201 caattatatt ggagtagaaa tgcaggtcag tgttgtttcg attgcactag acaagttgat
     4261 cgaacaaccg cttcctaacc tgaaacttct acatgtggat ggatcagcgt tgacagagta
     4321 tttcgctgac agcgaagtag atcaaatcta tctgaatttt tccgatccat ggccaaaaaa
     4381 acgtcatgaa aaacggcgat tgacatataa gacttttctt gctgtagatg aacagattct
     4441 tcgtccaaat ggagaaatcc atttcaaaac agataatcaa ggattgtttg agtattctct
     4501 ggcaagtttt tctcaatatg gcatgatcct caaacaggta tggttagatt tacatcaaag
     4561 tcaatttgaa ggcaatatta tgacggagta tgaagaaaaa ttttcttcaa aaggtcaacg
     4621 tatttatcga gtagaagctc aatttcaaga taagaccaaa aagaaaagtc tgtgaaacta
     4681 actttctgct ttcataaaaa cattaatatc cataaataaa tcccagaaaa tgaacattac
     4741 agatgtaatg tattttctgg gatttatttg atttaaaatg tatttatttc atcggttttt
     4801 ccacaaggtg gaatcaattg gaagcgagaa aatgaaaata atcctttgtc ggtgatcttc
     4861 aattcgggta tgacagataa tgttaagaaa gaaagtgtca aaaaaggatc aaaggtttta
     4921 gctgctccta gttgatgtgc tttagttatt aattcattca actgttcata gacgatcgaa
     4981 aaaggttcct gtgtcatgat tccgcctata ggaagagaaa cacaagctat ttcctgctgt
     5041 ttgttgacaa cggtcatccc gccacctttt tgaatcaaaa gattagcagc atacagcata
     5101 tcctcgtcgt ttgtcccaac gacaactaga ttatgcgagt catgggcaat cgttgtcgct
     5161 atcgctcctt gtttcagctg aaatcctttc actatgccta atccaatgtt tcccgtttga
     5221 tggtgacgct cgatcaccgc aatctttaat tggtcttttt cgattgaagg aatgaatcgt
     5281 tcgccttgtc ggtccacttt ttctaccaaa tctttagtca ctagactatt cgggataatt
     5341 tcgatcacat gggcttgtga agaagaaaga agcagctcta acgaatgtgg atttaatggt
     5401 tgagcgttta gttttggcag acaacacgta tatgaatttg aatgatcagc aaataaatgg
     5461 tcttggagtt gcccagattg aacaactaat gttcccttat agaaaacttc atgaattggc
     5521 agcttggtaa gatcgtctgt taagaagaaa tcagcttgat acccaggggc aatcgcacct
     5581 agtgttttca aatcaaaaca ttctgctgca ttcaatgttg ccatctgaat ggcttgtaat
     5641 ggatcgattc caagtgcaat agcttgacgg acgatatgat cgattgatcc ttcttttact
     5701 aaatcattga tcagtttatc atcggtgaca aagaagcatc ttcttgaatt ttcaggtgtg
     5761 atcgccggta gtagcgatgc cagatctttt gcaacggtcc cttctcgaac cattaaatac
     5821 ataccaagat ccaaacgttc tttagcttct tttgcagttg tagcttcatg atccgtccga
     5881 atacctgctg caggataaac atttaactct tccatgccga taccagctgc atgcccgtca
     5941 atttttttct tatgctggtg catcaattgg atcttttcta ggatgtctgt ttcattattt
     6001 gctactgctt gatagttcat gacttcagct aagcctaaaa cttcgggttt ttgtaaaaaa
     6061 ggggctaatt tttttgcatt taaatttgca ccattggtct caaaaggggt tacaggtaca
     6121 caagaaggta gcataaaaaa gatattcatg agtgtttgtc ttgcatcttc tatcataaac
     6181 tcaagacctg tactcccagc aatgtttcct atttcatggg gatctgtcac tacagtagta
     6241 actccatgaa gcaaggatac ttttgaaaat tcgcttggtg ataaaagtga gctttcgata
     6301 tgcacatggc catcgatgaa tcctggcact agatattgcc cattagcatt atgtacgctt
     6361 tttccttcgt attcaccgat tcctacgatc attccttcgc agattgctac atctttttcc
     6421 atgatttctt tcgtaaagac gttgacgatc ttagcatgct tgatcactag gtcagcaggc
     6481 ttttttttac ttgctacttc gatatatttc cttaatgttg tttccaatgg agtgcctccc
     6541 aagataaatt tttataagta taacataaat tcggtttttg ccaatatttt tttgtaaatt
     6601 cattttattt gaaacaaaaa gatcaaaaag acgtatgttt tttgtggagg ggagaataat
     6661 ggaaataaaa cgattatcgg atcaaatgat aagtgaaagt tttgccaaac atgcccaaga
     6721 cgtatatata ttgccagctt cagaagtatt ttacatctat tttcgctatg gaaaaaaacg
     6781 aacattctgg aagaaagtct cttcggcaga tggacagcaa ctgatctcaa gatttaagta
     6841 tttgggacag atggatatcg gagaaaaaag aaaagctcag ttaggtgcat ttacttattc
     6901 tttagaaaaa caggaagttc gtctgcggtt gtcttctgta ggagattacc ttcaacgaga
     6961 gagtttagtg attcggctat tatacggtac ttttgaaaca ccccatcgtt gctttagttc
     7021 tcgagatttt gcaactattt tacagaaaac cgtcagacgt ggattatacc ttttcagcgg
     7081 accagtcgga tcaggcaaga cttctctaat gtatcggttg gctttggaag aaaaattgca
     7141 agtgattact atcgaagatc ctgtagagat agaagaaccg ttttttctgc agctacagat
     7201 caatccgaaa atagcacaga cttatgatac acttttgaga ctcgcattac ggcatcgtcc
     7261 agacttgcta atcattggcg agataagaga tgaatcaact gcacatgctg cggtaagagc
     7321 agcactaaca gggcaccggg tttttgcaac cgtacatgca cgcaatcttg aaggtacatt
     7381 ggtcagaatc catgaactga caaagaaaaa agaagagcta tctgaatgtt tagctggtgt
     7441 catttatcaa gaacttttgg aagatgccaa tcaccatcct tctgttttat ggagttatca
     7501 atttatggag gaagaatcag taaaaaaatc atgggaggaa agttatgaag aagctcagga
     7561 aaatatttgg cagtcagaac tattctaaag gattaacaag aagccagcaa aaccagtttg
     7621 cacaaaccat gggagaactg cttttatctg gttttagtct gcaagaatcc ctttatttat
     7681 tactcacgat ccgttcgttt ccacctgtta ttttggcaaa tatccaacat cagctaaagg
     7741 aagggcaagc gtttagtgag gtactttcgc aaataggata tggagcagag caactggtac
     7801 agatcgagtt agctgaacgt catgggaatc tgccgacaac tttgttggaa attgccaaac
     7861 agtttcggct tgttgaagga tttcgcaatg atttgaaaaa gctgcttgca tatccgatgc
     7921 tattgctcac ctttttgcta ggtatcttgt ttagtatgcg gttgttcgtt ttgcctcagc
     7981 ttattggttc aggaatggtc atgcaagagc attggggtat tcagctgata caagtctttc
     8041 attggtatgt tctactattc atcctagcgg ttactctatt ggctttacta gtaaggtatc
     8101 gtctgatgaa gttgtctcct gttgatcaga gtgaatggat aagcagacaa ttattgattg
     8161 gaccgatata ttccttgtat caatcggctt atttgtcact agagattgga aagctttttc
     8221 atgaaggttt cgaattaagg caaatcatag tctgcttaaa ggaaacgaaa caagaaagtc
     8281 tgattcagca tcttgcctct aagttgattc aaggattgga ggcaggtatg tcattggctg
     8341 agcagttcca gaattatcgt tttctatcag aagaattcag taaaattgtt ttacagggag
     8401 aggcaaaggg aagtttaggt aaagaactga ttttttacag cgaatggaca agaaaacagc
     8461 ttttcaaacg tcttcatcac tggatgcatt ttattcagcc aatcattttt ttgggcgtag
     8521 cggctttgat tttattagtt tatgccgctg ttttattacc tgtttacgga agcatagagg
     8581 aggttttgcc atgaaaaaga gaaaaatatc ttattatagc ggttttacat tgatcgaaat
     8641 gctaatcgtc ttactgatca tcagtgtatt agtgttgttg ttcgtcccta atttgtcgcg
     8701 ttataggaat catgtagacc aagaaagtcg tgaagcgatc atccagttgg tagacacaca
     8761 aaaagagctc tatgcgcttc aaaataatgg aagagttcct actgtggagg aattgttgaa
     8821 tgaaggctat atcaaacgag aacatgcaga aatctatcag cggccttgaa ggtaaggttt
     8881 tagcatcagg atatacgttg tttgaatcac tcttactgat tttattactt acaacgatcc
     8941 tttcttttcc agttctcgca ttttctgcat ggaagaatga attggcagtc tatcaatttt
     9001 tcggccaatt tgaaaaacga atctattcta cgcaaaaaat cgctattgtg aatcagatcc
     9061 aaacaggctt ttattggaat agtgaagaga atcaaatcat ttttcatgtt ccgaatccag
     9121 ataaaataaa ttgggaagta ttagatattc ctgatgagat taccttgaaa cgccatgctt
     9181 ctattacttt tgctgctaga accgggaatg aaagttcact aaaagcttat caattttatt
     9241 gggaagaaaa agagcggacg attacgtatc aattccagat agggagtggt cgctatacaa
     9301 aaagaattga ataaaacagg atttgttttg ttggagtccc ttacagcgct tatgatcgct
     9361 tctgtctgtg tctacttagt gagctttggc cagctgtttt tgcttcaggc agaaaagaaa
     9421 agtggggagg aactgctatt tattcgaatg atttatgaag atatcaagac gcagcggatg
     9481 tatcacaaaa gaccagagcg aagggaagaa atgatcccat acgatatcca aattgtcgat
     9541 aataatggag taatcgaaaa agtgtttgtc aatatggaga aagaagcgat cagtattgaa
     9601 aaaacaagta attccagcat ttacgctgat tgaatgtcta attggactga tgatattgag
     9661 cagtctattg ctcacattca atcttttgat tcagcaaacc atccaagtaa gtgaattttt
     9721 gcagcgtaaa gatcaaaaag agtggctaat atttttagca caattagaag aagaactcaa
     9781 gagcagtcac aatgtctctg tgaaaaatca gcaactgcac tacacgatcg aggataaaca
     9841 gtatatcatc gaaagatatc aggcaatgat acgtaaaaga agaacttccg gcggacatca
     9901 acctatgtta acaagtatca ctgagcttca attattggaa aaagaacaca caatttcttt
     9961 ttatgtacac tttgaaaatg gagaagatgg atatgccata tggacaaaaa acgatcagta
    10021 gaaaatgtta taagggctct attttatttt ctgtcttgct tttatttctt ttatgtacgt
    10081 ttttattgat ggcagcatta gaaagctacc gtttgtcaat ggatcttaat atacgaatca
    10141 aaaattatta tatagcaaaa ataatgaagc agatggcttt atcggaacag tctacgcttg
    10201 ccgaaagttc ggaaggagtt ttttactaca caactggatc tgtaacatat cagtgggtac
    10261 aacagtccct attcttagag gtggaagtct caccgtttat ctttcggttc acagaagaag
    10321 taaaaaatga tacagatact agtacagaat gatggaatgt tcgtgaaaac gccctctttt
    10381 ctgttataac actgttttta ataaaaaaat gtttgccgtt tcacaaataa aaaacagtga
    10441 aaacgattga aattttgtca attgttcgat agaattaaac aggtgaaaaa attgttataa
    10501 tatatctaga aatgaggtgc accatgttac cagaaaaaat ggaacaaggg tttaatcaaa
    10561 acctggaagc tattcagctt ttacagaatg cattgggaac ttccttttta gaagcctatg
    10621 tcgaaaatgc agaaaattta atagatgatt atcaagtacg tgtggtggat ggagtgccga
    10681 caaaagagac aacgcaacgg atcacagctc tttatgaaga gttgaagaaa ctttctttcg
    10741 agccagaaga atggcgcaga ctgtctcaat tacttttatt gaagggcagc cagacagaac
    10801 atttgcaggc gaaccatcaa ctcactcctg atagtatcgg ttttttattt gtttttctta
    10861 ttgaacaact atacacaaat aaaaaagcac cggttaagat tctggatatt gcggcaggaa
    10921 tgggcaactt gttattaaca gttcttttaa acctatcgaa tgcggggtac caaacagaag
    10981 gaattggtgt ggatattgat gatacattgt tggctgtcgc agcttctaca agtgatttga
    11041 cacaagcaaa tgttcaatac tttcatcaag acgggttgca ggaattatta atagatcctg
    11101 tcgattttgc aatcagcgat ttgccaatcg gttattatcc gaatgacgaa aaagcaaaag
    11161 agtttttaac aagtaccgaa gaaggccata gttatgcaca tcatttattg ctagagcaat
    11221 caatgaaata tgtgaagccg gatggttttg gtttgttttt gatgccgagt ggatttttag
    11281 aaacggatca aagtgaagaa atcaaaaaat ggtttaaaga agaaggttat cttcaaggaa
    11341 tgattcaatt gccagatgaa ttgttccgta ataaacaatc gcaaaaaagt attttgatct
    11401 tacagaaaaa aggaccacaa gcacaacaag taaaagaagt cttgcttgtg aaacttgctt
    11461 cattaaaaga accagagaag gtgacggaat ttttcaacga gtttaaaaac tggaaatcat
    11521 ctgctctgta atcattcaac gagaaggaga gtcatatgtc taaaacaatt gcaatcaatg
    11581 ctgggagttc aagtttgaaa tggcaattat atcaaatgcc agaagaaacc gtagttgcga
    11641 aagggatcgt cgaacgtatc ggtttacaag attctatttt taccatcaag tacggcgata
    11701 atcaaaaatt cgaacaaatc atggatatcg aaaaccacga agtagcagta aaaatgctgt
    11761 tagatcagtt gattgaactg caaattttaa gctcttatga tgaaatcacc ggagtagggc
    11821 accgtgttgt tcacggagga gaaacttatg gggattcagt agtgatcgat gagaaagtaa
    11881 tggaccgcat tgctgaatta gcagaatttg ctcctttgca taatcctgca aacttaatgg
    11941 ggatcaaagc gtttaaaaag attttacctg acattttgag tgtggctgta ttcgatacat
    12001 ctttccactc aacaatgcca aagcaaaatt atttatatag cttaccggtg tcttattatg
    12061 aagatttcaa agtaagaaaa tatggcttcc atggcacaag tcatcgttat gtgtcggaac
    12121 gtgcggcaaa attattgaac aaaccgatcg aagaactaaa aatcatcact tgccacttag
    12181 gaaatggtgt atcgatcaca gcagtcgatg gaggtaaatc catggatact tctatgggat
    12241 tcacaccatt agctggggtg acaatgggta cacgttcagg tgatatcgat ccagctgttc
    12301 tcccatattt aatggaaaaa ttagagattg atattgatga aatgatcaat gttttgaaca
    12361 agaaatctgg tctgctaggc ctaacaggat tgtctagtga tatgcgtgat cttgaaaaga
    12421 actatgaaaa agaacatatt cgtttagctt atgacatttt cgtggaccgc atccgaaaat
    12481 acatcggcgg ttatgtgaca acgatgaatg gtgttgatgc aattgtcttt actgctggta
    12541 tcggcgagaa tgacggacat gtacgtagtg aaatcatcaa aggtatgtct tggtttggtt
    12601 gtgaaatcga tccagcgttg aatgaattgc gtggtgaaga agtagatatc tctacaaaag
    12661 aatcaaaagt aagagtatta gtgatcccaa cagatgagga actaatgatt gcccgtgatg
    12721 tggagcgtct gcgcggctaa gccaaagcat tcgttaggat aaaagatgaa acgcatgcaa
    12781 gagggaattc ttgcatgtgt tttttttgcg gagaagaccg caaaatggta atttaacttc
    12841 aatttccctt cttttttaca ggatcttctt agggaatttt gcttggaaaa gtggtcaaaa
    12901 gggaaaagac tggcatttta tttattgatt ttcaggaaaa tcccaatatt ttattttcat
    12961 tcatctttat aagggacatt acatatgtaa aaagaaaaaa catttttaga tcagcctcgt
    13021 gggctgtcga ctttattctt cgctttatgt ttagctagaa aaccgatccg aaacattcat
    13081 tgatcgaata ataaaaactt gattttccac ctgtactttg cctgaatcgt gaaagcacag
    13141 gtatgaaaat caagtttttt gttgcgaaaa aacgtcctag tcaatcagtt ggccgagcgg
    13201 ctggcggctg atctgctcct gatcaataac gagtgcttct tggaagtgtt gctgcaaaaa
    13261 gtagcggtca tggctgactg ctaggagcgt tccttgatag tcttgcagca tttcttcaat
    13321 ttcttctcga gcatagctgt cgaggtgatt ggttggttca tccaaaatca ggacgttgat
    13381 tttttctaaa aagagtttca gcaacgccaa acggattttt tcaccaccgg ataaatcttt
    13441 gatccgttta cccacatcgc tttgataaaa gccacttttg gctaattgcc gtcgagcgac
    13501 ttcttcgttt ccagtcaatg tgatggcgta agccagcaaa cggctatcgg gatcggcaaa
    13561 ctgcagctgc tgcggcaaat aaccgatttg caagctacta cctaatttga tggtgccacc
    13621 gtaaggcttt tcctcaccga gtaataattt gagtaaggtt gattttcctg caccattgtc
    13681 gccgatgatg gctaaatgat ctccacgata gactgcaaaa ctgctgtctt tgaatagtac
    13741 ttgatcagcg tactgctggt gcaagttttg aatgatgaaa acttctttgc cagattttcc
    13801 accattagac aagctttgca gccgattttt cgggggaggt ggcggtttga cgagtgtcag
    13861 tttcgccagg cggcgttcca gttctttggc ttttttgaag aaggactcat tgtctccttc
    13921 atgtgcccac tgacgaaaac gacgaatcat gactttcaac cgctggactt ctttttgctg
    13981 cagttcgtaa ttttttgtca gctcagccaa gcgagcttct ttcaagactc gaaaacggct
    14041 gtagtttccg ggatactcaa tcaatgcacc ttcttccagt tcccatgttt ttgtggtgac
    14101 ttgatcgaga aattgccgat catgagaaac gaccagaacc gatttttttg tattctgtaa
    14161 atagttttcc aaccagcgaa tactgttaag atctaaatga ttggtcggtt catcgagtaa
    14221 aagtaaatcg gcatcggctg ctaaaatttt ggctaaagcg actttaactc gttcgcctcc
    14281 gctaagttct gttaaccgtg cttcctgacg gtgtggaatc cctaagcctt gcaagatcgc
    14341 tgcaatccgg tcttctaata cgtaaccgcc tagttcttca aagcgatgct gcagctcacc
    14401 gtaacgggcc agacacttct ctaagtttgt atctggttct gtcatcaatg cttcgtagcg
    14461 actcatttgc tgtttgaggt cctgtatctc agcggcagaa tggagcaagt agtggttgac
    14521 ggtcgcttcg gaaactgtaa gttcttgttc gaccgtcccg atctttaagc cttttttgcg
    14581 actgataaca ccgctgtcga caccttctct gccggtcaag atttgtaaaa aggttgattt
    14641 gccagttccg ttgataccga ctaaggcaat tttttcacca gcttcaattt gacaggataa
    14701 atcctcaaat aatggattcc cgccgattgt ttttttaata gaaattgctt gtatgatcat
    14761 gatctctctc cttgagacca ctcaaaaagg ccatggttat ccatggcctg tgttatttaa
    14821 actcagatta tcatgagaaa aataccttga gatggccatt gattttacgt gaattcaact
    14881 gttttggaca aacttgtccc gtcaacagca actgtaaaac caattgcatg gctaaatgcg
    14941 atatggtatc gttggtaatt ttcattactt gttattttag tcatgcgcat catagtattt
    15001 tccctccttc gtgtagttgt cttttccagt gtacctgaaa gtgtcagaaa gttcaactag
    15061 cgctgacatt tacttaatat ttatcgatgt gtaattctgt cagagagctt tttttatctg
    15121 actgagggta atgtctcgtg gaccacatca ttgtattctg agctaaacca gcgttcaggc
    15181 gaaatcatat ggtgtttgat aaaaaacttt gtctacatga cttccttttg ccatttgctt
    15241 aataccgttg cattaaacag ctcttctctt tggaaatctc accaacatcg cgttcaggag
    15301 tatcgttgtg gttgcaatgg caagcggtag cctttcatca attcttgaat gatttaccga
    15361 atgtagcact tgccttctcg cggtacccga acccgaaata aaattttagt ttggcgataa
    15421 aatcggtatc cgccatcgat acttcatatt ttttcgtatc agattcatcg gaaacttcca
    15481 cattgacgaa ccaataagct ttggcatgct taggtcgttt taaaccacac cagggcgact
    15541 aatcgccctg gtgttcactt ttttttattt tacttgaact cattgactgt tttcccttta
    15601 ttttaaacat tataaagacc gatgagtgct tgccgcgtct tttgttttta tcacggtttt
    15661 ttgtgcaaaa aaacctgccg atagttggca ggtcgtctac gtgttaagct ttggtgtggt
    15721 ctttagctgg caattggttt tctaaatcag ttcggacaac aagcacatcg caagctgcgt
    15781 ttctaataac atattcagaa acagaaccaa taaataaacg ttctacagca ttcaatcctg
    15841 tggctcctaa cataatcaaa tctacttgat tatcttctgg gatttctctg gcaataattt
    15901 gttttggtga gccatattct acgacagagg ttacattatt caagccagct ttcttcgcat
    15961 tgctttcata atctgccaat gtttgctttg ccatttcagt agcttgttct gcaagcattc
    16021 catcaaagga tgaaacggtt tggaaagaac gtgtgtcaat gacatgtgct aatagaagtt
    16081 ctccgttatt tcggatagct acattgaccg cctttttaaa agctaattca gcttcatcag
    16141 aaccatctac ggcaaccatg atttttttgt attgttgtaa cataagcatt cactcctcaa
    16201 gtcaatcgta ttgtactctt ttattgtaga acactttagg gaaaaagaga attatttact
    16261 gctttggttc agcaattttt tttgcaaatc ggatactgaa ggtggcagaa acaataatta
    16321 cgatcagtaa aaagaccaag gcaataaagc gttgattgaa aaaagctgtt aaaataccca
    16381 ttacgcctaa aacagcatat gcagctccgt aaaattgtag gaatccttga tttttatcat
    16441 ttttgttgat caatacaaaa aagatatcct gtttattaaa taaataatag ctaacgaata
    16501 ataatactaa agtggcgata atcattaaaa tcctaatcat gaacaaatcc tccaaaataa
    16561 gcaacaaata gaaaaagcaa tttgccaaga gcaaattgct ttattcttta aaagatggta
    16621 atctccgtta atgccgttgt ttgtcctgta gtattgatcc cgcgaattca agcaggtggg
    16681 tcccatggtc gtagcttcga actaccaaat gataaggcat gttaccagca gtgacacaag
    16741 acgggtccca agatatcaat aaaaatgttc ggtcaacaca caaagaggac aattcgaaca
    16801 tcacagattt cccacctcta tagtagcata tgaatttaaa aagagcaatg agagagctcg
    16861 cagaaacaga accgttttct tgtctagttt tttggaggat gctttttcca ctcttttata
    16921 cggtaatact gttgacccag agcctgttca tatttccctg tatcttttgg ctgataatac
    16981 tgagcattct tcagtttatc tggtaaatat tgctgattga cccaagcctg atcaaaatga
    17041 tgaggatact gataacccac gccacggttg agtgatttcg cacctttgta atggctatct
    17101 ctcaaatgat caggaacatc tccagctttg ccctctcgaa tatcagcaag tgctgcatct
    17161 aatgccatgt aggcagaatt ggattttggc gacaagcaaa gatcgacgac gacgtctgct
    17221 agtgggatac gtgcctcagg caatcccaat ttttcagcag ctaatacagc gttcactgta
    17281 cgagccgcag cagcaggatt acctaaacct atatcttcat aaccgataac catcagacgc
    17341 cggcaaatac ttgccagatc acctgcctca actaaacggg cgagataatg aagtgcagca
    17401 tccacatcac ttccacgaat cgatttttga aaagccgaaa taacgtcata atgagcatct
    17461 ccatttttgt catgggtaag agcttttctt tggacacatt cttcaatgat cgacagtgta
    17521 agatgaatcc ttccttcttt atctgaagga gtcgatagtg tagccagttc cagcccattc
    17581 aatgcactcc gcaaatcgcc gttcgttgca cgcgaaaggt gaagaagagc atcttcgtct
    17641 aactggattg cttgttgtcc aaggccgcgt tctttatccc ttaaagcatg ttcgactgcc
    17701 aactggatgt cttgttctgt taatggtttg acttcaaaga tctgtgtacg gctgcggatc
    17761 gctggattga tggtaatata aggattctca gtagtcgctc cgatcaagat gattcggcca
    17821 ctttctaaat gaggaagcag aaaatcctgt tttgttttat ctaatcggtg tacttcatcc
    17881 aataagagga tcactgtacc actcattttt gcttcttccg ctacaacttg tagatccttt
    17941 tttgaatctg tagcggcgtt tagcatgcga aatgcataat tagtagagcc ggcgatcgca
    18001 cttgcgatac tcgtttttcc agtaccagga ggtccatata gaatcatgga agagagcatg
    18061 cgagcatcga ccatgcggcg tatgattttt ccttccccga ctagatgctg ctgaccaacg
    18121 acttcatcga tcgttcgagg tctcatgcga taagccaatg gctgttgcat aggattttcc
    18181 tccttcttgt ttttacgtcc cttaattata tcatatttag caagatgcct agaaagaaca
    18241 gccttctgtt gttttgtttt catagcttat ccaactaata ttgtgtatga taaagtaggt
    18301 gagaaaaatg acggataaac aaaagataga tgaatttttt tggaataaaa cgacgccaga
    18361 agtggcacgg gatttattgg ggatgtattt ggagcatgac acaccagaag gaagattagc
    18421 tggatatatt gtagacgcgg aagcatacct aggtccagaa gacgaggccg cccatagcta
    18481 tggtttaaga agaacaccgc gtgtgcgggc aatgtatgaa aaaccaggta cgatctacct
    18541 ttacacgatg catactcatc ggattttgaa catcattact cagccagaag ggattcctca
    18601 aggagtaatg atacgtgcaa tcgaacccgc cgcaatgatt gatcaaatga gtaaaaatcg
    18661 tggcggaaaa actggtccag atatcagcaa cggtccggga aaattagtgg aagcattagc
    18721 aattcctcaa gaactttatg gtcagtctat agcagatagt tcgttacgct tagtttttga
    18781 gaaaaaaaag acaccaaaaa agatcaatgc tttgcctaga atcggtatcc caaacaaagg
    18841 tgtatggaca gaaaagccgt tgcgttttgt tgtgtcggga aatccttata tctctttaca
    18901 aagaaaaaac caaatcgaga aagattgggg ttggaggaaa gaaaatgaaa aagaaggatc
    18961 tattaacata tttagatgaa ataattgaaa aacaagtgac agactatgat gtagctttag
    19021 attgggatac aaaaaatcat acgatcgaga tcgttgttcg cttgtttgca gaaaataaag
    19081 cacaatttga gattgatgat gcagaaggga tcgtttcgca agaaccaatt atcgaatttg
    19141 aagacggcgt acttcttttt aatcctcaga aatctgtttt tgatgaacgc aattatctag
    19201 cagtaatccc ttatgaagga aaaaaagggt tggcaaaatc agtagctgat tctttagtgg
    19261 aatatctaaa tgaagtatta gctcaagggc aaagcgattt gcttgatttt ttggatgaag
    19321 acgatgacga agctgtattc gagcttcact gggataatca gcaactagca gaattagtag
    19381 aagtaaaaca gcaaaatgaa gcagatactt atttaccgta cccaagttat taaggagtga
    19441 cagatatgaa atggaatgaa gtaaaagtag aaactgaaag tgaagcagtc gaagcagttg
    19501 ctaatatttt gatggaggca ggggcgagtg gtgtcgcgat tgaagattct ttagatgttg
    19561 aacattttca gtcggatcaa tatggtgaga ttttagataa aaatgatttt acatcgctga
    19621 aagaaggcgc actagtcatg gcttattttc cggaaacgac tttcttaccg gaaatcttgc
    19681 cttttatcaa agatcgtatc acacaattac ctgagtttgg tctagctatt ggaaaaaata
    19741 aagtaacagt tagtgaagta gaggaaagca actgggcaac agcctggaag aaatactatc
    19801 atccggtgcg tattacgcga atgctgacgg ttgcgccaag ctgggaaaac taccagacag
    19861 aagatcctct agaaaaaatt atcacactag atcctggtat ggcatttggc acaggcactc
    19921 atcccacgac ttccttgaca ttgacagcac tagaaacagt cttgcgtggt ggtgaaacat
    19981 tgctagatgt gggaactggg tcgggcgtgc taagtattgc agcaaaatat cttggtgcaa
    20041 aagatgtcta tgcctttgat ttagacgaag tagccgttcg ttcggcgaaa gaaaatatgg
    20101 atatgaatga aatagccaaa gatgtccatg tatcagctaa cgatcttttg acaggaatcg
    20161 agattgaaag tgatgtaatc gttgcgaaca ttttagcaga tatcatctta ttgatgatcc
    20221 cagatgcttg gcgtttactg aagcaaacag gcacgctgat cgtttcagga atcattgaag
    20281 agaagaagca aatggttctg gatgccatgt tagaacaagg gtttatcgtg gatcaaattt
    20341 tccagcaaaa agactggtat gcaattattt tgaaaaaacc agaggtggaa tagatgcagc
    20401 ggtatttttt aaaagaatca tatgaatcaa aagactattt tgttttagaa ggggaaaact
    20461 atcaccacat tgtacgggtt atgaggatgg aacccgagga tgaagttttt ttggcattca
    20521 atgatcaaat agccattgtt gcagaaatca cggagattac tgaagaggct gtttatctga
    20581 aagaaattaa aaaagaagaa aatcaaaagg aactgccaag ttcgattacc atagcgagtg
    20641 gctatcctaa aggggaaaag ctggattgga tcgttcagaa atccactgaa ttaggagcgc
    20701 aagcttttct tggtttcccg gcatcagcct ctgtcgttaa atgggatgca aaaaaacgta
    20761 tgaaacgaca acaacggctt gaaaagattg ctcaagaggc agcggaacaa tcccatcggc
    20821 agatcgtgcc atcagtagag ttattggaaa cgaaacaaga tttatttgag cggctagatg
    20881 attatgacgt ggtcttggtt gcttatgagg aatcagcaaa gcaaggcgaa aaatcaaatg
    20941 cagccaaaat tcttagtgaa acaaaaccag atagtcgaat cttagctgtc tttggaccag
    21001 aaggtggatt atcccgcgat gaagtcgatt attttgtaga acacaaaggg atactttgtg
    21061 gattaggacc aagaatcctt cgtacagaaa cagcacctct ttatttatta agtgctgcta
    21121 gctatcaatg ggaattaatg aatccttctt tcagctaacg ttcttcatta ataaaagagt
    21181 atcgacagaa agaattgaaa aaatagtcaa caatttgtat aatagaaaca attatttata
    21241 caatggatac gaaaaatcgt ttgaaaaagc ttttaaaaag cgaacatttc cgtaaaaaaa
    21301 cgtataactg cacggatttt gggacaatta tcaaacagat atttgtcccg attcccgttt
    21361 taccgaaaaa agaaaggggg atcgattcct gtgacaaaag aagaaatttt aacgggaccc
    21421 ggtgtcataa agctcgttag tcaatacatg ggtcccgaac atgtagcatt tgttgagaaa
    21481 gcatgcgaat acgcgactgc tgcacatgac ggacagttta gaaaatcagg cgaaccctac
    21541 attattcatc ctatccaagt cgcaggtata ttagcagatt taaaaatgga tccccataca
    21601 gtggctacag gcttcttaca tgatgttgtt gaagatacag aaatcacttt agaagatctg
    21661 agagaagaat ttggcgatga cgttgctatg ttagtggacg gcgtaaccaa attagggaaa
    21721 ataaaatata aatcccacga agagcagcta gcagaaaacc accgaaagat gctgctagca
    21781 atggctcaag atttacgagt tatcatggtc aaattagctg atagactaca taacatgcgt
    21841 acgttgaagc acctgcgaga agataagcaa agaaggatcg ctcaggaaac tttagaaatc
    21901 tatgcgcctc ttgcacatcg tctagggatc agccggatca aatgggaatt agaagataca
    21961 gcacttcgtt atctaaatcc aaaacagtat taccgtatcg tccacttgat gcagacgaag
    22021 agagaagaac gtgaaaaata cgtaagtggt actgttgaag atattcgaat agcgacagaa
    22081 gagctgggga tttttgcaga aatctatgga cggccaaaac acatttattc aatctatcgt
    22141 aaaatgaaag atcagaaaaa gcagttcaac gaaatttatg acttgctagc tattcgagtg
    22201 atcgtagatt caatcaagga ttgttatgct gtattaggag caatccacac aaaatggaaa
    22261 ccaatgcccg gcagatttaa ggattatatt gccatgccaa aagctaatat gtaccaatct
    22321 ttgcatacta ccgtgatcgg tccggcagga aatccggtag aaattcaaat ccgaacacag
    22381 gaaatgcatg aaatcgctga attcggggtt gctgcacact gggcctataa agaaggaaaa
    22441 aatgaaaaag tagaaccaga tggtatgacg aaacaattaa gctggttcca tgagatactc
    22501 gaacttcaag acgaaagcta tgatgcttct gaatttatgg aaggcgtaaa aggagatatc
    22561 tttagtgata aagtctacgt cttcacacca aaaggagacg ttactgagtt accaaaagga
    22621 tccggaccat tggactttgc atacagtatc catacagata tcggtaacaa aaccactggt
    22681 gcaaaagtaa atggcaaaat ggtgcagctt gattacaaat tgaaaaacgg agatatcatt
    22741 gagatcatga cttctccaaa ttcatttggc ccaagtcgcg actggttgaa attagttgct
    22801 actagcaaag caagaaataa gatcaaacgt ttcttcaaag cccaagatcg agaagaaaat
    22861 gtgatcaaag gccacgaatc cgtggtcaaa tgtattacag atctaggatt tacgcctaaa
    22921 gatattttga cgaagaacaa actgcaagaa gcactcgatc gttttaatta tcaaacagaa
    22981 gatgatctct atgcagctgt agggtatgga gaagttagcc ccttgacgat ggccaatcgt
    23041 ctgactgaaa aagaacgtaa agaacaaaaa atcgagcagc aaaagcaaga agcagaagaa
    23101 atcatgaatc agccgaaaaa agaacctgac aaaatgaaag tacgtcatga aggtggcgtt
    23161 gtcattcaag gggtagaaaa cttactaatt cgtatcagtc gctgctgtaa tccgattcct
    23221 ggtgatgata tcgttggtta tatcactaaa ggcagaggga tatccattca tcgtcgagat
    23281 tgtccgaatg ttcagcctga caaaccaaat gtagcagaac gtttgattga agtcgaatgg
    23341 gaagatacat cgaatacacg aaaagagtat gatgcagatt tggaaattta cggctataat
    23401 cgttcaggct tattgaatga tgtacttcaa acagtcaatg cgctaacgaa aaatctcaac
    23461 agcgttgaag cacggacgaa taaagataaa atggcgacga tccatttgac ggttggtatc
    23521 cagaatttat cccacctaaa gagtatcgtg gataaaatca aagcagtacc tgatgtctac
    23581 agtgtacgcc ggacgaatgg ataggaggac agatatgaga gcagttgttc aacgggtaag
    23641 taaagctgct gtgtcagtcg atcagcaaga aattggaaaa attggctacg gcctacttgt
    23701 tttattaggc attcatgaaa ccgatacgca aaaagatgtg gactatctgg taaaaaaaat
    23761 tgcacaaatg cgtatttttg aagatgaaca aggaaaaatg aatattagtg tagaagatgt
    23821 gggaggacaa attttaagta tctctcaatt cacactgttt gcggatacga aaaaaggaaa
    23881 tcgaccaagc tttgtggaag ccgcacgacc agaaacagct attcctttat acgaatcatt
    23941 caatgaagga atcagaagtc gcggcatcac agtcgctacc ggacaattcg gagcagatat
    24001 gtcggtttct ctcgtcaatg atggacctgt aacgatcatt attgattcta aaaacaaata
    24061 aagtaatctg gttttcacta gtaaaaacta tgaaacacgt cgcttaagtt agactgataa
    24121 actctaactt aagtggcttt tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa
    24181 aaaagttaag cttaaattat acaagttatt ttttatagga ggttgctgaa gatgaaagaa
    24241 gtaccagtag tagatatcaa aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac
    24301 atgttggagg agttacgcca tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat
    24361 aaagaagcta tcacgatcta cggatcatca gcagcaaaag tattttatga tccaaggaat
    24421 ttcaaacgaa aaggtgctat gccaaaatta gtattgaaga cactttttgg tcaaggtgga
    24481 gtacagactt tggatggagc tgctcatcac catcggaaaa atatttttat ggatctaatg
    24541 acaccggaaa gaatggaaga ttatcatcgt atcttagata agaatctgac acaagcactt
    24601 gaagcacagc atggtcaatt tgaactattt gatttaagta agatggtttt ctttacatct
    24661 atttgcgagt gggcaggtat caatttatca gcgatttcta aagatgaggt cgaaaaatta
    24721 gcagaatatc aaatctctat gatcagcgga acgtttactt ctccgattga tcacataaaa
    24781 ggtgtcgaaa atcgcaaaaa atcagaaaaa tgggcgcaag gcttgattga agaagctcga
    24841 caaaatcctg tggctggaaa agaaaatgtg gcattatatg cttttgccaa tgccacagat
    24901 ttagatggtc agcttttacc tctggaagtg gcagctgttg aattattgaa catcatccgt
    24961 ccaacggttg ctctaactgt gtgggcggca ttgatgggac atgcactttt cagtagacca
    25021 gatttgtatc agcaattaaa aaatgacttt tcgacgttac aagatccgtt tattcaagaa
    25081 atgagacgct attatccttt cttcccaatg ctacctgcga tttcgctgaa agaagtagaa
    25141 gtagatggtt atcgtatacc agaaggcagt tgggttattt tagatttata tggaacagac
    25201 catgatgaac gaacagttga agcgccagat tcctttatga tcaaacggta tgtagggaaa
    25261 gcaaaagata tttcctataa agaagaatat gagatgattg cacaaggcgg gggaaatttt
    25321 cgacaaatgc atcgctgtgc aggagaatgg atcactcttc atagtctacg tgtgttttcc
    25381 gatcagctag taaataagtt tgagttctct gtacctgaac aagattggac gattccattc
    25441 aatcagtttc cgacttatcc gaatagccga gcgctacttt acaagaatta gattagcata
    25501 aacttatcga aaaaaaggaa gatcaaacag tgctgatctt ccttttttca tattactata
    25561 aattgataag gtcaattgat caaaatccac gaaatgcttc atcaatggtt tgataatctt
    25621 cattggataa atgcaaatct aaagcttgga cattgtcaga tacttgctct ggacgcttag
    25681 ctccaggtat aactacagac actcgtggat tcttcatgta ccaagctaaa acaagttgcg
    25741 caggagtggc ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt
    25801 ctctgaaacg ttctccttgg aaatctggat ttctttttcg aggatctcct tcaccaaatt
    25861 gtttttcctc accaagttca tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg
    25921 gaacaaaact gattttgttt ttttcaagat aagggaataa ttctttttca gcctgccgat
    25981 ggatcaaact gtatttatct tctactacat cgacatatcc atctgcattc gcttctttca
    26041 attgttctaa cgtgaagttt gacacaccga ctgctcggat ttttcctgct tcttttaatt
    26101 cgtgcaatgt agcaactgat tcattttttg gtgtgctttc atccggaaag tggatataaa
    26161 aaatgtcgat gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa
    26221 actcaggaga attatcaaat gttcgccctt tatttggtac atgagcagct tttgtagcga
    26281 tgacgacacg tgagcgatca tattcttttt ccttcagtac ctcgccaatc agttcttcgg
    26341 aacggccgtt tccgtaagca taagcggtat cgatcaaatt gatcccgcta ttcaaagctg
    26401 tacgaactac ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa
    26461 cggcatttgc tcctaatcca agctgttctg cgtagacttg tgaatgtccg attcttattt
    26521 ctgccatagc tgattcctcc tttttgtcta gtctaaaaca aaataccaaa aagctgaact
    26581 aaaacgtttt tgtgagggga aatagtcaga tatctcttga ctttgagact ggtttttagt
    26641 aggttataag taatatcgaa tgtcaatgaa agagcgagta agtttttgaa actttacaag
    26701 agaggatcat cgaggctgaa aatgatctaa agtttcatta gcctaaagac actctggaga
    26761 tggacgagtg aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa gagaaatctt
    26821 tcaaaattag gtggtaccgt gtatgaattt cattcaccct tgttcatgat gaacaagggt
    26881 gattttttta tttacatagc taagaaaggg agagaaaact atgagtttcc aaagaccaaa
    26941 aggaacaaat gatattttgc caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc
    27001 acgtttatta ttcagagatt atcagtataa tgaaatgcgt acaccgattt ttgaacacta
    27061 tgaagtgata tcccgaagtg taggggatac aacggatatc gtttcaaaag agatgtatga
    27121 tttttatgac aaaggggaac gccatgtgac gcttcgacca gaaggtacag caccaatcgt
    27181 tcgttctttt gttgagcata aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt
    27241 ttatatggga ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac gtcaattcca
    27301 tcaaatcgga gtagaagcat ttggatcaga aaaccctgca actgatgtgg agagcatggt
    27361 gatggcactt gatttcttca aacagttggg gatcacgcaa atccggctag tcatcaattc
    27421 attaggtgat aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact atcttttacc
    27481 ttttgaatca gagctgagtg aagactctaa acgccgcctt catgaaaatc cattacgcgt
    27541 attggacagt aaagacaaac gagatcaaaa atttgtggca gaagctccat ctatcttaga
    27601 ttatctaagc gagcctgctc aaaaacattt tgataccgtt gtttccatgc tggatgcatt
    27661 ggctgttcct tatgaaatcg acagtaatat ggttcgcgga ctagattatt acacacatac
    27721 gatttttgaa atcatgagtg acgcaccaaa aatgggggca caagcaacga tttgcgcagg
    27781 cggacgttat gacaatctag tggaagaact aggtggtcct tcaacacctg gcttcggatt
    27841 tgcaatgggt atcgagcgat tactcttgac tatggaggca gaggaagtag ttatccctgc
    27901 atttaatgag ttggatgctt acgtggtcgc actcggagac gaaacaaata tcgaagcatt
    27961 gaaagtggtc caagcgattc gaaactttgg tttttcagct gaccgcgact tcatgaaccg
    28021 taaagcaaaa gcgcaattta agacagcaga taaagcgaat gccaaattag tattggtgat
    28081 cggtgaagat gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga
    28141 aaaaagtttt ccattagaag agatatatga acattttgat gaagtctatg atgaaatgac
    28201 tttattcgag taggaggaaa aaaataaatg gcacaaagaa cgatctattg cggattagtt
    28261 tccgaacaac aattagaaca aacagttaca ttaaaaggat gggtacaaaa aagacgagac
    28321 cttgggggag taatctttat cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt
    28381 aatcctgaaa tcaacaaaga agcatgggaa atcgcggata aatgtcgcag tgaatacgtg
    28441 atcgaagtga ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc
    28501 actggtgaat tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg
    28561 ccatttcaaa ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat
    28621 ttggatctgc gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc gacaacaaaa
    28681 gcgattcgtc attatttaga tgataatgac tttctcgata tcgaaacacc ttacctaggg
    28741 aaatctacac cagaaggcgc aagagactac ctagttcctt cacgtgtcca tgctggacat
    28801 ttctatgcgt tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa tgctggattc
    28861 gaccgttatt atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag
    28921 cctgaattta cgcaagtaga tattgaaaca acgttcttaa cagcagaaga aatccaaact
    28981 tacacagagg gattgattgc gaaggtaatg aaagaagttc gcggtatcga agtcacattg
    29041 ccatttcctc gtatgaccta tgatgaagca atggctcgct acggcagcga caaaccggat
    29101 acacgttttg atatggaatt gatcgatcta agcgatacag tcaaagaagt agaattcaaa
    29161 gtcttccaaa tggctcttga aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct
    29221 gcagatcgct actcacgaaa agacatggat cagttaggtc aatatgtagg acaattcgga
    29281 gctaaggggc ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa
    29341 ttcatgggag aagcaacaga agcaatcatt aaagcaacag atgcaaaacc aggtgacttg
    29401 ctgatgtttg gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc tatccggaca
    29461 cgtttaggaa aagaattagg actaattgat gaatcgaaat tcaatttctt atgggtaact
    29521 gattggccac aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca
    29581 ttcacgatgc caaaagaaga agatatcccg ttattggctg aagaccctgc aaaagtctat
    29641 gcagaagctt atgatatcgt cttgaacggt tatgaattag gcggaggttc tatccgtatt
    29701 catactcgtg agcttcaaga aaaagtacta gaaactctag gctttacaaa agaacaaatg
    29761 gaagaacaat ttggtttctt gttaacagct ttagattacg gattccctcc acatggcggt
    29821 attgcattag gattggaccg ttttgcaatg ttattagccg gagaagataa tattcgtgaa
    29881 gtcattgcct ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc accaagtatg
    29941 gtttcgcctt tacaattgtt cgaattgaat attgatgtaa cgaatataga agaatagccg
    30001 ataaaaaggt aaaaaggatt tgtcgaatac gcttcttttg acaaccttga tttaattgaa
    30061 aaacacttta taatgaagcc ataacatata aagcggggct tttccatgaa aaaaacattc
    30121 gaaaattggg cttatcatga ttacacgacc aagctttcgg tatctatcgt ttatgcgttg
    30181 atcgcatcag tcgcattgaa tttcttttat cagccaggac atatttattc aagtggtatt
    30241 actggtctag ctcagatttt gacgactttg tcaaaatcac tgataggatt cgaagtaccg
    30301 gtatcaacaa ctttgtattt actgaatata cctttgtttg tattggcgtg gttaaaaatc
    30361 gggaagaagt ttacaatttt cacttttatc acagtcactt tgacctccct ttgtatccat
    30421 attttaccgg aaacagtcct atctaccgat ccaatcatct gtgcgttgtt tgggggggct
    30481 gtgatgggac taggtattgg gctagcgttg agaaacggcc tatcatcggg cggtctagat
    30541 atcgtgagta tcacgattcg taaaaaaaca ggaaagtcga ttggttctat ttcgatctac
    30601 ttcaatgcat tgatcgtttt tgttgccgga attctatttg gctggcaata tatgttttac
    30661 agtacgttgt ctattttcgt cagtggaaaa gtaactgatg tcgtttatac taaacaaaag
    30721 aagatgcagg taatgatcgt aacgaaacat ccagaaaaag tagtaaatga aatccaaaac
    30781 tgtatgcgta gagggatcac gatcatcaat gaagcagaag gtgcttataa gcatgataag
    30841 cagacagtat tgatgacgat cgttacacga tacgaactgc ctctacttag agaagcaatg
    30901 aaagcttctg atccacaagc ttttgtaagt attgcagaaa acgtccagat acttggtcgt
    30961 ttctatgagg atggcattta gatcgagttt gacaaaaatt catgaaatac tgctgtagac
    31021 agacgttcgt ttgtctgcag tttttttcta ccatagtaga atcagcaaag aaaaactgct
    31081 tttttatttg aaattttctt aaagttgaaa aaaagcatgt ttattttttt aaatttatgg
    31141 gatgatagaa aaaaatagta gagaaaggac acgattctac ttgctatttt cacttaatac
    31201 ccgtatatta cgaatgaaaa aagtttatga aaagttggaa tattgaatgg aaaaacaaaa
    31261 aaggttaaaa aacagccgct acatcactgg gtttgatggg ctccgttcat tagctgtggt
    31321 gggtgttatc ttctatcacc ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc
    31381 gatttttttc gtcgtatctg gttatttgat cacggatctg ctgcggcagg aatgggaaca
    31441 aaatggaaaa atcaatattt ggcaatttta tgtcagaaga atgaagagac tctatccggc
    31501 acttgttttt atgcttgtga cagcttcggc ttatatcact ttgtttcagc gagggctgtt
    31561 aaacaatctt cgaggagttg tggtcagcag cttactgtat gtcaacaact ggtggcaaat
    31621 caataacggt ttatcttatt tcgatcgatt tgctaatgag tcaccgttta cgcatatctg
    31681 gtctttagca gtagaaggtc aaaactattt ggtttggccg atcttgtttg tattgttgat
    31741 ggtatttgtt cgtaaaagaa aatggatctt ttatactgtg ttaggtacat ccttcctctc
    31801 cgccatcttg atgatgatct tgtatacacc aggaggcgat ccaacccgtg tttattacgg
    31861 taccgacact cgtctgtttt ctatatggtt gggaagtgcg cttgcgtttg tttggccaag
    31921 tacgcgttta aagaaaaaca tcccgaaaca agcgaaaaat attctgaata tcgctggagg
    31981 aatctcgctt ttggcactga ttctttgctt cttcttttta gatgatcatt acaatttcat
    32041 ctattacgga ggaatgctcc tagtgagtct gttatgcgta gtgctagttg cagtgacagc
    32101 acaccccgga gcaagtttga atcgatggct gacaaatcca gtatttactt ggattggcaa
    32161 acgaagctat ggcatttatc tgtatcagtt tccagttatg attttttatg aagcaaaggt
    32221 gactgatatt gccgatcatg ttttgttgca tactttagtg gaaatcacat tgattctaat
    32281 catcagtgaa ctttcttatc gatatgtgga gcgtccgcta gcacgttttg attatggtcg
    32341 tacattccaa gtgatgaaag gatggttcag caaaccgatc atcagtaaga gcaaaccatg
    32401 ggttgttcca ggcacattac ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa
    32461 aaacgctgta acagccgatc aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt
    32521 agcagaagaa accagaaaca attcagaaaa caccactagt tcggttgacc aagcgatcct
    32581 tgataagtac gagttgtcca aggcacaagg gaataaggca caaaaattag aactaacggc
    32641 atttggtgat tcagttatgt tggatgctgc aacggatcta caagaacttt atccaaaagt
    32701 agtggtagat ggagatgtcg gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt
    32761 aaaggaacaa gatttgttga aagacactgt tctgattgga ctaggaacga atggctcatt
    32821 ctcggaagct cagtttgata gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt
    32881 caatgtccga gtaccgacga agcgctggca aaatgacgtg aatgcgatgc ttgaacagat
    32941 gacgaaaaaa tacgacaata tgacgctgat tgattggtac agctacagta atgaccatag
    33001 tgactggttt tatgatgatc aagtccatcc aaatcctgaa gggatgacac attacattca
    33061 cttagtcagc gaagccttgc taggaggaag ttcaagtacg aacagcagtg agacaaacgt
    33121 gacagaatga tagctacaaa aaacttcagg caaaaacatt tgtttttgcc tgaagttttt
    33181 ttatatttac cgtgttttta acattcatat agattcagga agcaaaacgg ttgagtgtgt
    33241 ctgtaaataa cgttcttttt gttttcgggt gagttttttg aatgcagctt ctgcttttgt
    33301 tgcttcgctt cttgaagaaa atacttctgc atggatcatc ttaacaggtc ttcttttttg
    33361 aagacgagta tatttagcac cagtaccatt gttatgttcc tctaatcgtc tagaaagatc
    33421 ggttgtatat ccaccataaa atgtctgatc tttacagaat aatacgtaaa aataatgttc
    33481 atttgccata gagcatctca cgaatctctg cagtgtattc tccatccata ttgtaagtga
    33541 tcaatggtgg agcgactttg aatccgtcta atttgccttc tttgattcct tcaactaaca
    33601 aaatatttgc ctcttttcca ggttttgggt aaacaaattg gatcctttta ggtgcaattc
    33661 gatagtcttc catgacctga atgatttcta agaaccgatc aggacgatgg accatagtaa
    33721 agcgtccatt tgtttttagt agcttacttg aaatatcgat cacttcttct aacgtcgtat
    33781 gaatctcatg acgggcgatt gccaggtgtt tattagggtt cgttttattt gttggcagcc
    33841 ctttgaaata aggtggatta cagacgacta agtcacaaga attatgtcga acgacagata
    33901 agctgtcttt cagatcgatg gtgtggacag tcatttgttc atttaaatgg ttaagcgcga
    33961 tactacgtct tgccatgtct gctagacgag gttgaagttc gatagcgtcg atagccgcct
    34021 gggttcgttt acttaaaaaa agtccaactg cgccatttcc tgcacaaagg tcaacaatct
    34081 tccctttttt aggtacacgg gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa
    34141 acactttgga actttggata atcttgatat cttctgcgta taattggtcg attcgttcgt
    34201 cttcgtgaag catggattcc ctccaaaatg aaaaaaatag tcgtctgtat tatactattt
    34261 ttattaaaaa gaaccaaggt ggcttgtcaa acctctattt tttatgcata ctatgaccat
    34321 aagaagggaa aggaagagac tatgttttat cgatttatgc gcggggtagt tcgagtacta
    34381 ttagccttag ttaatggaaa tgcccgctat gagaataaag aagttttacc gcaagatgag
    34441 aattacatac tagttgctcc ccatcgtact tggtgggacc cattatatct ggcggtagct
    34501 gcagcaccca aggaattcag ctttatggca aaagaagaac tgttcaaaaa tccagttctg
    34561 cgatttatat taactcgatc caatgctttt ccagttaaaa gagaaaatcc aggaccaagc
    34621 gtcattaaaa cacctgtaaa gattttgaaa tcgacaaatt tgagtttgat catgtttccc
    34681 agtgggacaa gacatgctac agaacttaaa ggcggaatgg cattgatttc cagaatgtct
    34741 aaagcgaaaa tcgtgccagc tgtatatcaa ggtccgttga cacttgggga tctgttcaag
    34801 cgtcagagag tgacagtgcg tttcggcgaa ccaatcgact tatcagatat caagaaaacg
    34861 gataaagaag gtatggcgga gatcgaacga cgaacacaag cagcttttga caaattggac
    34921 aaagaagtga accctgattt caaatatgaa gtcaaaaaaa aataaaatga taagtttatg
    34981 cgtgggcggt cttttgaatc tactgtcctg ttcagttcag actttttcaa tgaaaacgag
    35041 ggagagacgt caagtctgcc ctctttttgc gttccaatat gtaaaataga agacaggtgt
    35101 catgtaatgg atcaaagtgt tataataatt agaaggacat tttttgaggt gattgaatgc
    35161 gttttttaca tacagccgat tggcatatcg gaaaaaaact acaaggttat gacttgattg
    35221 aagaccagcg tcatgtacta tcttctatac tagatatcgc gaaagaagaa aaagtagagg
    35281 caatcgtgat tgccggagac ttatatgata ggagtgtccc agctgttgat gccgtagaat
    35341 tatacaatga attgatggtc gattggaatt taaaggaaaa gatcccgatt tttgctatat
    35401 caggaaatca cgatagcagt acgagacttt cagcgggtac gccatggttc agttattcga
    35461 actatttttt gcgaacacag ctagcagaag catttgaacc agtcgagttt aaaaacgtac
    35521 aatttttctt tcttccttac tttgaaccaa ttgcagcgag attatatttt gaagatgaca
    35581 atatccgaac gatcaagcaa gcggtagcca aggtgataga taaaatgcag gagctatttg
    35641 attcagaaaa aaaacaagta ttgattagcc atttttttgt aatgggaagt gtaaaatctg
    35701 attcggaaac gaaaatcgaa gtaggcgggt tggattcgat ccctggtgaa ctacttacac
    35761 cgtttgatta tgtagcacta ggacatttgc ataatcaagc tgctttgaga caggataatg
    35821 ctcgctatag cggttcccca ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg
    35881 tatggatcgt tgacctgaca acaggcctgg agttgaattt tagggagatc gaacccctgc
    35941 atgatattga agaaataaca gcgagtttca aagaattgct ggatcctgcc ttttatcagt
    36001 ccgtcaatcg ggagaattac atctatatct atctcactga tagaacgatc atcccgaata
    36061 tgatgaacca gcttcggcaa atatatcctc gtattttggg gattgaacgg gcaaacggtc
    36121 gagcagaaca aacaaagcgc aaacgaaaag tactagaaat gaaagatcct caaaaattag
    36181 cagaagcatt ttttagtgaa atgacggaag agcagctaac tgcaaaccaa gaaaaatggc
    36241 tcggagaaac gttgcaagaa atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat
    36301 tacaacttaa aaattttggt ccttttatca atgagaccgt agatttttca cgtttgacag
    36361 aagcaccgct ttttttgatc agcgggaaga caggagctgg caaaacgacg atttttgatg
    36421 ggatcacctt cgcacttttt ggtgaaactt ccggaagact tcgctctgga aaagagatgc
    36481 gttctttgtt tgctacacca gacgaagaaa caagcgtgac tttttctttt gaacatcagc
    36541 agatgagcta tgaaatcgaa cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg
    36601 gaacaaaaaa acaggcagcc aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa
    36661 taaaacaaat cacaaaacgt acagaagcag atcagttgat cagagaactg atgaatctcg
    36721 atgcgaaaca gttttctcaa atcgttttac taccccaagg agaattccgt aattttttgg
    36781 tttcatcaag cagtgataaa gagactgttt tacgtcattt attcggtact caatttttcc
    36841 aacagttcaa tgagcgatta aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt
    36901 tagagcaaga gctgcatctt ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag
    36961 cagcagggtt tgaagccgtt ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac
    37021 aaatcacgga agaaaaagaa gctctaacgg aacttcagga aaaacaaaag caactagaaa
    37081 attcctacta tcgttttgaa agcttgaaaa aaagttatga agaaaaaacg atattaataa
    37141 acaagcaaaa ggaattagct gctcaaaaag acgaaataga ggaaaagaaa cgatggattg
    37201 ggtattatga atttacgcaa cgattagtcg aaccaatcca gcgcctacaa gaaataaaag
    37261 ttgaaaaaat agaactgtca caaaaaagta gtgagcaaac aaagaaacta gcgatgacta
    37321 cgcagaatta tgaagagtgg caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa
    37381 tcaaagaagc tgctcaaaaa ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct
    37441 tacgtagtaa aaaacaagac gcagaacaat taacaatttc attggataaa gaagaattga
    37501 atgccgagaa gctgtatcaa aatctcgcta tctatcagga aagaactcga attcttcaag
    37561 aaaatttact ggatcaggaa aaactgcaag aagatcgtct tgcttttgga cagttaaaat
    37621 atctagatga aaagcagcaa gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg
    37681 cacagataag agaagaactc aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat
    37741 tgaaagaaca gacagaacaa gcagaacagc tgaaaagcca atgggcaaag cagcaaattg
    37801 ctcgtcttca gctttttttg cgtccagggg agccatgtct tgtttgtgga tcaacggaac
    37861 accccataca acaattaaaa cacgaacagc caaataagga agatatctta aaagtagaac
    37921 aacaactggc agaagctgag tctgctgtag aaagaactca gcaaatcatt gccaaaagtg
    37981 aagcacatga aaataatctt caacaacaat tatcagattt agaacaaaaa atcatggcaa
    38041 cgaatataca attggctgaa caagaagaac acttgcaaca ggctttgaaa aaggcatttc
    38101 ctgagataac agaacaaaaa acaaaaacaa ctcttgtagt aatcgaacaa tctttagata
    38161 gacgaaaaag caagatggcc aatgcaagaa tagagctgga agaaataaga caacaagcag
    38221 aaaagctcga acaacaatta gctgtctaca aacagcagca gacagaaaaa aataatcagc
    38281 tccagcaact gcaaggagaa atcaaaagtt tagaacaaca attgcaagat acggatagta
    38341 actgtttaga agaggagata cgacgacttg aaaaacagtt agaagaagac tcgaatcaac
    38401 tagctgttga gcagaaagca ggagtgcaat tgttgcaaga tcttcatacg cttcggacac
    38461 agcttgatct tcaacatgaa caaatagaaa aaatagatga aaaaatagaa aaggaacaag
    38521 aaagaatagc taaaaagtta gaaagccagt cgtatttttc aacaattgaa gaaatctgtg
    38581 catttgcaca acagcaagca caatataaag aaaaccaacg tacagttact tcttatcatg
    38641 aagagcaatt agtgacagct gaccggctgc gtcaattaga aaatatccag ctaactgaga
    38701 cattcccaga tacagaagag atcagccaac agttagaaga aataagacgt atgatcgaaa
    38761 taaagcaaga aaagatatac ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg
    38821 atgaatgtct ttcgatttat cacacaagcc agacgaaatt agatgagctt acgcaattac
    38881 agcagctttc gcaaacattc aacggagaaa atcctaagaa gactagtttg gaacggtatg
    38941 tgcttcaggt ttatcttcaa gaagtgttac aagtagcaaa tgatcatctc caacgtttaa
    39001 cgaaaaatcg ttatcaattt gagctagcgg ataccattgg cagttatcga gggaaaaccg
    39061 gattagaaat caatatctat gatgatcagg caggtatgtc tcgaagtgcg cataccctgt
    39121 ctggcggtga gagttttatt gcagcacttt ctttagcatt agctcttgcc gatgtgatcc
    39181 agacacaagc tggtggtgta gcaatcgatg cattgtttat tgatgaagga tttgggtcat
    39241 tagatgaaga agctttggaa atggcaatgg aagctttaga aacgatcgaa agtgaaggaa
    39301 gaatgatcgg catcattagt catgtaagag aattgaaaga acgagtatta caacaaataa
    39361 gaattgatac agaaggtagc ggacaaagca aaatacgcta tcaacttgga taaaaaggga
    39421 gattggtaac tatgaaatgg agtattccag aaaaagtcat tgcacgtgga cgtcaatact
    39481 tgcaagaagg ccgagtgtta tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag
    39541 aagtcttggg cagtgaattg tatatggttg atttggatgg tacagcaaaa gagatagatt
    39601 actgccaatg tccctattgg gaggaacata aatattgtaa gcatacagtt gctgtggaac
    39661 tttatttacg gtcgaaaaat ctatctcgta tcatgaaaaa agaccagcca atgctagaac
    39721 cggtaaaagc aacaagtgag ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac
    39781 aaaacaatcc tcaagcggtg caacctttga ttattgaata ccaagtagaa acgattgaga
    39841 ctaatcagta tcacccagag ctttctattt tggcacttta cttgcgtatc ggttcattgg
    39901 catcaccgaa aaaaacatat atcgtaaaaa acatctatga ttttttacaa gcttacacag
    39961 aaaaagaaac ttacactgtt aataaacagt accagtttcg tctagataaa cgggcgtttc
    40021 aaaaagaaga tcaaaaaata ctcacgcatc ttgcaggaag tgctcagacg catcaattat
    40081 taggtacgaa cggcttacaa gtcaaaggaa aactagataa aagatatctg cttctcccag
    40141 tagaacaagc ccgcttcctg ttagagcaga tgaatcagac tacacgtttg catctgcaga
    40201 taaatgaaca aaacgatcaa ggaatacaat tttccgatca aaacaaacct ttatcattca
    40261 aagtggaaaa atttggagaa gattatttgc tgcaagcatt gaatgatttt gattttttct
    40321 ttatgcatta tcagtgggga gttatccaag ggacatttta ttctctgacg aaatatcaac
    40381 aaacggttta tcaaacttgg aaacaattga ttcgccgatt ggagaaacca gaagtagtgt
    40441 tcaagaaaaa agaattaccg tctttgttta aagagattat ccctctgctg tcagagatcg
    40501 gcgacgtagc tattgcccct gcagtagaag aggaagtagc agatatcccc gtcgaattta
    40561 ttttcttttt ccgaaaagca aaaggaaaaa tccaagctcg agtcgatttc gtctatggtg
    40621 aagtcatcta ttcgactgac ccgaagcatg aggtctcgac agaacaggct tttcaggtgt
    40681 tacgtgacac agtagaagag aaacgagtac ttgatctatt cagaatgtat cgctaccaga
    40741 aaaatgaaac tggttatgaa cgtccattgc caagcgggga agaactatat gcctttttcc
    40801 gcacagaact atctgtcttc cgtcagtttg gtgaagtcag acttggaaga aaattgcgag
    40861 agttgtattt agatgcgcat aaacatcaac cgaaaatcga agtaaccgag accggttcat
    40921 ggctcgatat tcgattcgat gtgacaggga tagaggagca agaaatcgat catgtcctgc
    40981 aaagcttgct gagaaatgat gctttttata cattagaaaa tggtcaggtt ctttcttttg
    41041 attcagaaga atttcaacaa acaagccaag tgttgcaaca attacgggaa tcgatacgta
    41101 cagaagaagg aacgatccat gtacctaaaa atcaaggatt gatcattcaa aatcaattgg
    41161 aaaaaagcaa tgctactttt agtgaatcgt tccaaacgat ggtacaagat ctcattcatc
    41221 cagaaagata ccaggcacag cttcccaaag gattgaatgc aacgatgaga gattatcaaa
    41281 agcaaggttt tcgatggttg aagatgttag gtcattatca gtttggcggt attttagccg
    41341 atgaaatggg acttgggaaa acactacaga caattgcctt tttactttcg gaaaaagaag
    41401 aacggaaaag cttctctgct ttgatcgtag cgcctgcgag cttaatttac aactggcagg
    41461 cagaggtgag aaaattcgcc ccttctttaa gtatccaagt aatcaatggg aataaaaaag
    41521 aacgtgaaga attactggct aaagacacag atatccgagt gacttcttat gccagcttaa
    41581 gacaggatct agctaattat cagtcgcaaa aaattgatta tttgattctt gatgaggcac
    41641 aaatggtcaa aaatagcagt acgaaaactg cacaagcatt gagagaatta gctgtacctc
    41701 agcggtttgc actgagtggg acaccaatcg agaataattt agaagaattg tggtcattgt
    41761 ttgcaacgat tatgccagga tttttcccga ctaaaacaaa attcagggaa cttgccgcag
    41821 aagaaattgc gcaaatgatt cgaccgttta ttttacggcg agataaacaa actgtgttga
    41881 aagacttgcc ggaaaaaaca gagatgaatc tatattctgc acttacagaa gaacaaaaaa
    41941 ctgtttattt agcttacttg cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag
    42001 cattcaagaa aaaccggatc gggatacttg ctggtctgac aagactgcga caaatttgct
    42061 gcgatcctcg tctgtttatt gaagattacc aaggcggttc cggcaagctc gaacaagtaa
    42121 aagatctact ggtagctgcc aaagagaaca aacgaagagt tttgctgttt tctcaattta
    42181 cagggatgct gactatcttg caagaagagt tagccgagct agggatctcg acattttatt
    42241 taagaggcag tacgaaacca caagatcggt taagtatggt agacgcattt aatgctggcg
    42301 aaaaagatgt ttttctgatt tccttaaagg ctgggggaac tggattgaat ctcacgggag
    42361 cagatacggt catcttgtat gacctttggt ggaatcctgc cattgaagaa caagctgctg
    42421 gacgtgcaca tcggatcgga caaaaaaatg tggtagaagt gtggcgtatg atctctgaag
    42481 gaacgattga ggaacgcatg gattcactcc agcaagaaaa acgcgaactg ttccaaaaag
    42541 tgatccaagg taatgaagaa cagttgacga aactgactga agaagacatc cgcatgattt
    42601 taagtgtagg agaaatcgag gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa
    42661 agcctttcta aagaagcgat tgtttatttg atttaaaaaa tagcaaggag gctgggattc
    42721 cattttatcc cagcttcctt gctatcattt ttttatttct atttttaaag gttatacaaa
    42781 gattgtcttc attgtctcta acagctgatc aaaagaagaa atctcgatat cgcaaattgg
    42841 agtctgttca attttgcgtt ctcgatggtt gaaccaaagc gcttgccagt tagcactttt
    42901 tgcacctaga acatcattgt cgtaattatc gccaacgtat aaggtctctt ctggaagcat
    42961 atgaaattct ttctcagcca gctgaaaaat ctctttttct ggtttttgga aacctgttgc
    43021 ttgagaaatg agcatattgc gggaaggaat ccaattgttc aattgaagtt gcttcagttt
    43081 ttttgtctga tggtcagtcg gcccatttgt gataattcct aatgggacat tcttttcctt
    43141 caagaaatcg agggtttttt tgacctcctc atgcaagcag atattatcaa gttcttcctc
    43201 gtaaattttt tgaaataaga ggccatcttc ttccgtaata tgagggtagt ctaaatcttt
    43261 tagtgattgg ctgatccgat gtgctcgcat atattctagt gtccaatctc ctgccattac
    43321 ttttgggaag ttttcatcac tatgatggcg gaaccggata tatagtggat gcatatctgc
    43381 atctgaaaca agcggaacga ctcttttgac cgcgtttcga aatggctgct gctgatcata
    43441 catcgtatca tcaacatcaa acacaactga tttaatcaaa attttacacg cctttcggtt
    43501 ttcattgatt ttttcacaaa tccttgacta ttgtaacaga aaaaattgtc taaaaaagct
    43561 gataaataaa ggtttttctt actatatttc tatttccttg acagaataag gtttttatat
    43621 ttcagttttt ttcactaaaa atcgtgaaac acgtcaataa gtttgatgaa caaattaatc
    43681 attatggtag aagtcgattg atttatttta atgcaatctc tgtggaattt tcgaaag
//
LOCUS       DASJUF010000010        43272 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJUF010000010 DASJUF010000000
VERSION     DASJUF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.010"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="U4B62_000498"
     CDS             complement(<1..132)
                     /locus_tag="U4B62_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2140681.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..781
                     /locus_tag="U4B62_000499"
     CDS             542..781
                     /locus_tag="U4B62_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140682.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            848..1057
                     /locus_tag="U4B62_000500"
     CDS             848..1057
                     /locus_tag="U4B62_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140683.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            1117..1788
                     /locus_tag="U4B62_000501"
     CDS             1117..1788
                     /locus_tag="U4B62_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN2140684.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1841..3817
                     /locus_tag="U4B62_000502"
     CDS             1841..3817
                     /locus_tag="U4B62_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2140685.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3830..4585
                     /locus_tag="U4B62_000503"
     CDS             3830..4585
                     /locus_tag="U4B62_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2140686.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4582..5406
                     /locus_tag="U4B62_000504"
     CDS             4582..5406
                     /locus_tag="U4B62_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140687.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5707..7806
                     /locus_tag="U4B62_000505"
     CDS             5707..7806
                     /locus_tag="U4B62_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2140688.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7844..9901)
                     /locus_tag="U4B62_000506"
     CDS             complement(7844..9901)
                     /locus_tag="U4B62_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEN2140689.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(10015..10227)
                     /locus_tag="U4B62_000507"
     CDS             complement(10015..10227)
                     /locus_tag="U4B62_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140690.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            10414..10881
                     /locus_tag="U4B62_000508"
     CDS             10414..10881
                     /locus_tag="U4B62_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140691.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10878..11120
                     /locus_tag="U4B62_000509"
     CDS             10878..11120
                     /locus_tag="U4B62_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEN2140692.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            11138..11440
                     /locus_tag="U4B62_000510"
     CDS             11138..11440
                     /locus_tag="U4B62_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140693.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11512..13566
                     /locus_tag="U4B62_000511"
     CDS             11512..13566
                     /locus_tag="U4B62_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140694.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13566..16178
                     /locus_tag="U4B62_000512"
     CDS             13566..16178
                     /locus_tag="U4B62_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN2140695.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            16175..18706
                     /locus_tag="U4B62_000513"
     CDS             16175..18706
                     /locus_tag="U4B62_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140696.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18758..19090
                     /locus_tag="U4B62_000514"
     CDS             18758..19090
                     /locus_tag="U4B62_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140697.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19091..19684
                     /locus_tag="U4B62_000515"
     CDS             19091..19684
                     /locus_tag="U4B62_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140698.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19847..21541
                     /locus_tag="U4B62_000516"
     CDS             19847..21541
                     /locus_tag="U4B62_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN2140699.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            22229..24106
                     /gene="ltrA"
                     /locus_tag="U4B62_000517"
     CDS             22229..24106
                     /gene="ltrA"
                     /locus_tag="U4B62_000517"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2140700.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            24237..24614
                     /locus_tag="U4B62_000518"
                     /pseudo
     CDS             24237..24614
                     /locus_tag="U4B62_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24614..25819
                     /locus_tag="U4B62_000519"
     CDS             24614..25819
                     /locus_tag="U4B62_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2140701.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25835..26479
                     /locus_tag="U4B62_000520"
     CDS             25835..26479
                     /locus_tag="U4B62_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140702.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26490..27296
                     /locus_tag="U4B62_000521"
     CDS             26490..27296
                     /locus_tag="U4B62_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140703.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            27318..27548
                     /locus_tag="U4B62_000522"
     CDS             27318..27548
                     /locus_tag="U4B62_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140704.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            28094..28369
                     /locus_tag="U4B62_000523"
     CDS             28094..28369
                     /locus_tag="U4B62_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140705.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            28411..28833
                     /locus_tag="U4B62_000524"
     CDS             28411..28833
                     /locus_tag="U4B62_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140706.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28852..30444
                     /locus_tag="U4B62_000525"
     CDS             28852..30444
                     /locus_tag="U4B62_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140707.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30456..30791
                     /locus_tag="U4B62_000526"
     CDS             30456..30791
                     /locus_tag="U4B62_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140708.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30938..31132
                     /locus_tag="U4B62_000527"
     CDS             30938..31132
                     /locus_tag="U4B62_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140709.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            31164..33536
                     /locus_tag="U4B62_000528"
     CDS             31164..33536
                     /locus_tag="U4B62_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140710.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33597..35057
                     /locus_tag="U4B62_000529"
     CDS             33597..35057
                     /locus_tag="U4B62_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN2140711.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            35068..37272
                     /gene="topB"
                     /locus_tag="U4B62_000530"
     CDS             35068..37272
                     /gene="topB"
                     /locus_tag="U4B62_000530"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2140712.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            37288..37437
                     /locus_tag="U4B62_000531"
     CDS             37288..37437
                     /locus_tag="U4B62_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140713.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            37511..38104
                     /locus_tag="U4B62_000532"
     CDS             37511..38104
                     /locus_tag="U4B62_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN2140714.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            38120..39019
                     /locus_tag="U4B62_000533"
     CDS             38120..39019
                     /locus_tag="U4B62_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN2140715.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            39022..39507
                     /gene="ssb"
                     /locus_tag="U4B62_000534"
     CDS             39022..39507
                     /gene="ssb"
                     /locus_tag="U4B62_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2140716.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39873..40133
                     /locus_tag="U4B62_000535"
     CDS             39873..40133
                     /locus_tag="U4B62_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140717.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40574..40780
                     /locus_tag="U4B62_000536"
     CDS             40574..40780
                     /locus_tag="U4B62_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN2140718.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40780..41031
                     /locus_tag="U4B62_000537"
     CDS             40780..41031
                     /locus_tag="U4B62_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140719.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            41046..41483
                     /locus_tag="U4B62_000538"
     CDS             41046..41483
                     /locus_tag="U4B62_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140720.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            41476..42183
                     /locus_tag="U4B62_000539"
     CDS             41476..42183
                     /locus_tag="U4B62_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140721.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42564..42743
                     /locus_tag="U4B62_000540"
     CDS             42564..42743
                     /locus_tag="U4B62_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140722.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(42883..>43272)
                     /locus_tag="U4B62_000541"
     CDS             complement(42883..>43272)
                     /locus_tag="U4B62_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2140723.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT        15895 a         6878 c         8838 g        11661 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaatagta
      541 gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag gaaggtatgt
      601 acagctgtta gctcgtggtt attcacctaa agaagtattc aaagccttat ctaaaggaac
      661 agatatggaa aaagaaagat tgcgaaaaga attcgattat tggagaaccc ataatggaat
      721 caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa cccaagactg
      781 aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa ggaaggtagg
      841 aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaag aaatggaaga
      901 aaagttacta aaaacgatga agcaaaaaca ccttaaaaga ttatctgtcg cgcaatatat
      961 caatgatatg cagttaacag gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt
     1021 tgaacagttg agacgaactt atagaattca tttttaaaac tttttctatg gaaatgagaa
     1081 aaagtttttt tgttttgtct gaaaggaggc agatggatga aaaaatggtt atttggtttt
     1141 ttaggagtca ctttaatcgt cttttgcagt gtgtttggct atgttagcta ccaaaaacat
     1201 gaaggagagg tgtttaagca aaatatagaa aaaaagatgc ccgtagatca aatcaatgca
     1261 catgcaaaga gttataagga ggatgcaacg aacgtcaata atgatatgtc tttggggcaa
     1321 atgttgtcca tacaaaaaga agcaatcgaa atgggcgtca ataaacaagt atttgcacaa
     1381 attcagattc ctgcacttgg tttagcattg ccaattttta aaggggcaaa tcaatacact
     1441 ttgagtcttg gagcggctac ttatttttat gaagatgccg aaatgggcaa aggaaactat
     1501 gtcctagcag gtcataacat ggaaatgcca ggtgttttat tttctgatat tcaaaagttg
     1561 tcattagggg aggtgatgga cttagttagt aacgatggcg tctatcgtta caaggtaacg
     1621 cgtaaattta tcgtacctgg gtactttaaa ttaatcgatg gcgttcctga agagaactct
     1681 ttcttgtctt tgccaaagaa aggagagaaa cccttattaa cgttgtttac ttgtgtttat
     1741 acgtcacaag gaaaagaaag atacgtggta cagggagagc tacaataaca aggttttaat
     1801 caacgcgagt cgttgaaata tgaaatgagg agagtaaaaa atggcaataa atcggtatgt
     1861 aaaaaaagct ggaatgcttg ttggaatgtt aggaatcatt gcaggctgct ttcaggggta
     1921 tcaagtacat gcagaagatg tcacgcaaaa aacaccacca gagaaggtca atatcacggt
     1981 tcataaatta atgtacgatc aaggaacgca attgaatgtt gatattgacg ggatcaaaaa
     2041 cgatggttat acgcacgatc aatatcctac aggtgtaacg aaatacaaca aggcagatta
     2101 tggtgatgtt gagtttacgt taggaaatat tacggatcaa gtattaccga gagaagattc
     2161 tgatctaacc aacgcgaaag tggacgagat tgtacaagat gtagaagaca agggatctaa
     2221 cagtgagtac gtcaagaatg caaccaacaa aattacttca gctgtagatg aaaatggtga
     2281 gatcactttt actgatcaac cagcctatgt caattccaaa gggaatgtat atgtggttta
     2341 tgaaagtaaa tccgctgctg gattagtcac acaaaaagca aaaccaatgg ttgtgattgc
     2401 gccaatgaca gataaaacag gatcaggatt tttaaaaaat attcatttgt atcctaagaa
     2461 cattgtttct aagttgagtt ttgaattaac gaaatttggt gacgatggcg cagaccaaag
     2521 caaacaaacc cttttaaaag gggcaaaatt tgagttatac aagggcgaac ctggaaaagg
     2581 aacaaaattg ggtgatcttg tatcggatga tcaaggaaag ctgactgcga ctgatttgac
     2641 gttaggaaaa tactattttg tggaagttcc ttcggaagtt gtagtgggat cagatcaaga
     2701 accaacagca gatcaatatt tattaggcgc agacgctcgc aacgatgcgc acaataaatt
     2761 aacctttgaa attacgaatg atggcgtgac aagtgactta aaagcttcat acgtaaacta
     2821 taaagcacca gtgatcgata aaacagtaac aaatggaaca ggacaagaac attctttcca
     2881 aattggtgac gcagtaaatt atcaaggaac gattcatatt ccaacagaca ttgcaggtgg
     2941 cgcagacggt atcacagtaa acggtgtgaa aagcgaaact tctccttaca gtgtatttaa
     3001 atggggagat acagcaggta aaggactttc ttatgttgct gaaaaagcag atattaaagt
     3061 aacaaataat gatggttcag tcgtattaaa agaaaatact gactataaaa tcgaaaattc
     3121 tgatagtggt tttgtgatcg attttatcgt aaataatgga caagtaagtg atacagttgc
     3181 gaatttacat ggtcaagatc taaaaatgac ttataacatg tttgtgaatg atagtgctga
     3241 aatcgcaaat ccattaacaa atagcgtgga tttcgtttat aacaataatc catttaatca
     3301 ggaagaacac cacgaaaaaa cgaaagctga cgttgtgact tatggcgcaa aattcttaaa
     3361 agtcgatagc ggcctatttg gaacaggtat caaagcaaca ccgcttgaag gcgcagaatt
     3421 tgcagctaag aatgcagaag gtaagtacta cggtggactt actgatacag ataaagatgg
     3481 tgtcaaagaa gcagtttggg tagacgatgt agcaaatgca gcaatcctaa aatcagataa
     3541 agagggtcat tttgaaatca ctggtttaac tgaaggagat tactcactgg aagaaacaaa
     3601 agctcctgaa aactatcaaa aattaacaaa agagatctct ttcaaagtag acaaagattc
     3661 atacaaagaa gaaaaccgaa tcacgatcaa aaataatcaa aaagcttccg taccaatgac
     3721 tggtagcaac ggtttccaat cttacgtatt gatctcttgc ttgttgctag gagcaggggg
     3781 actttctgca gttgtttact ttaagaagaa agcctagaag gatcagctaa tgattattcg
     3841 taagccccca aaaagaagag tcatgcacaa actgatgata tttttttggt tgctgtcggc
     3901 agtagcagtc tatttaattc cagttgtgga aacaaatgta gcgaaatttc aacaacagca
     3961 aagaatttta gccattgaaa atcttgaaaa aacagattct tctttggatg ttgcgaaaat
     4021 agagttaggc gatcctgttg gtgttcttac aattccaagt atttctttaa aactaccaat
     4081 ttatgacgga acttccgaca aaattttaga gaatggtgta gggattacgg aaggaactgg
     4141 cgatattaca ggaggaaacg gtaaaaatcc tttaatcgcg gggcacagtg gactttataa
     4201 agataatctt ttcgatgatt tgccttcagt aaaaaaaggg caaaaattct ttattaaggt
     4261 aaatgaagaa caacatgcct atcaaattta tcgaattgaa gaagttaaag cagaagaatt
     4321 acaaaaaaat tatttagatt atcttatgcc tgaaaaagac caagatagga tcacgttaat
     4381 gacatgcaca ccaaaaggtg tcaactcaca ccgcttttta gtgtatggtg aacgagtttc
     4441 cttcagtaaa gaagatttaa aactacagaa aaaagcaaaa aataggaaat caatttcatt
     4501 ggattggcga ttagcactgc tattcgcttt tttttgcgtt cctattattt ttatttataa
     4561 aaaaataagg aggagatcgg aatgaataag agaaaatggc tgtttgtttg tgtagcagct
     4621 ctcttttcta ctgtagctta ttcgcaactg tctttcgctg acagcagtga tagtagtacc
     4681 actcaaacaa tagataattc aattactgca ccaagcgaaa cggacagttc ggagaatact
     4741 acagaagaaa cggcagattc aaatataagc tttactgaat caacgaatcc aacgtcagaa
     4801 agttcttcag agacttcaga aaagccgcaa gacgtagaag atacgcaagt gatcaagcaa
     4861 gccattcata ttcaaaaaat tatttcttcg aatcaaatca acaatcaagg cagtcaagta
     4921 gcccagcaag aaggtatcaa tggcgcgaag tttattgtct acgatatcac gaatatctta
     4981 gaagaaatgt cacaagatga taatgagaaa aatcaatctg tagaaataat tgagtcaaaa
     5041 ttgaaagatc gagcaaaaaa actttcatac gaccagttga aaaaagtggc tgaaggagaa
     5101 accaaaacga ttgatgatca ggaaggcgtg attgaatttt ccattgaagt acctgcaaac
     5161 aaaaaacaag catactatat cgttaatgaa tcaagccctg aaaacatttc aaacagtgaa
     5221 gatattgttt tattgacacc tgtttcaaat gaaaaaggtg aatttttgac ggatgtttgg
     5281 ttatacccta aatcagagaa atcagagccg aaagaagaaa ttaaaaaagt tgtgtcaact
     5341 ggtgtaaaga agaacttttt tgaaaattgt tgggaatttt ttgctcaatt attttttgat
     5401 aattaggatg aaatgatgaa aataataaga atattagttt ttaatctatc ctttttagcc
     5461 gcaacaatat tgtgttgcgg tttaattttt tctcaattat caatttatcc aagatatcaa
     5521 cgattgttca ttcattttct aaacattcaa tgtgcttcca tgccattaga ggtgattaaa
     5581 aagctcttga tcaatccaat ttttatggtt agtttggttc ttcttacagg attggtaacg
     5641 tgggtattag gaaaaaaaga atgaaaagct tacgatcgtt taaaaaaata ttagatggag
     5701 ggcattatgg aaaataaaat tcattgcact acgggaagag taattacttt agtgttgttt
     5761 ttgttccttc ttctttttgc gaggggaact gcagtccagg ccttagaaaa agttgatccg
     5821 agtcaaatca aagaaaataa taatctggtg ctttatcatg atagtgattc attgagaaat
     5881 atcgaagtaa ctgggatcaa tggatttaag atactagatg agtattcaac taaaaaacaa
     5941 aaagtacttg cttggccaaa agtcaaagat ttgaaaagta cgtcaaaaat cgttgcccat
     6001 tatcaaaaag taggtactta tctaggaaaa gaaattgatt gtgacgtgac tttttctaat
     6061 tttgtagcgc acgctggggt taaccaagac ggattagcta aaacaattaa tggcaaaaag
     6121 tattataatt atttgctctt tgataaaaat atttatcgag gatttttcat gcagtcaatc
     6181 aatgtcatga atgcaaaact agaatttaag tatgcaggaa caaaaacaaa agtagctttg
     6241 agtgaaccac agccagaaat tcaatcaaaa atgagatcga tgagagcagc tggtattgat
     6301 ttaaacgata catttctatc catttacagt ttaaatggac agtatcagca acgtgtgtct
     6361 tggtataatg acaataatga atctgtcggt gaacgtaata ttgatatgag agaatttgtt
     6421 gggtatgaaa aaatgggtag cttaccttat tttgtcacaa cagatacagc atgtgcagaa
     6481 tacaaagctc ctgcgttatc aacaaacaat ttaacttcaa aagtagtggg aggacgtgca
     6541 gaaaaggctt atagctcgaa tgatcatttc accgatgttg taggagctga tacttatcac
     6601 agaagtggtg taacgtatac gcttcaaggc gcttccccaa cattcatgat tggcgcaaat
     6661 acgaatagta tgatgtttag ctttgatact gcattgctat ggacaccaca accatcgaag
     6721 cctacaaaag aagtgtttaa caaagctaat actgaagagg cagcacacaa tattgacaaa
     6781 aaagtgattc cacaaggatc agatgtttac tatcatattc accaaaagtt tgatgcatta
     6841 acagtcaaca caatgaacaa atacaaatca tttaaaatca ctgatacctt tgacagcaaa
     6901 aattttgata tggtatcgga tgggaaaaac tatgatggcg cattgtggta tgttgaagcg
     6961 aatggtactt ggaaaaaagc tgatacagga ggaaagttaa ctgtatcagg taatacagtc
     7021 acttatactg cttctgacag ctttttagct aacgctgcaa attacggtag ggattatatt
     7081 ttgcagctcc atatgagagc aaaaaatgat gcaaaaggaa ttgcaccaaa tgtggctact
     7141 gtaacgttta acaatgatcc gaacaaagga acacaaaaaa caaatacagt agaaaactat
     7201 ttctctctta aaccaaataa aaaagtggaa caagatggga aagatgttga tggtcgaaat
     7261 gatggtaaaa aaggaacgcc tacagctcct ttaaatgcag gatctgaagt acaatatcta
     7321 gtcacacaaa aatggcatac aaaaggtgta gacattgcct cagatcatta caagcaattt
     7381 agtattcaag atccagtgga agatcgtttg acgtataaag aaggttctgc gcgagtgata
     7441 gatatgagta caggaaaaga tattacttca gaaggtacac taacctatga tagcaattca
     7501 agaacgctga agtgggaagc ttccgctgat ttcttaagta aaaatctttt agatggacga
     7561 gaaattcagc tgatatttac agctaaaact cctttgaatg aagaattgga tatcgacaac
     7621 caagcaaaag tttcagtaga aagtattgag aaagcaacta atgtagtaac gattggggta
     7681 aaacctaact tacctcagat cattgtgcca aaaactggat cgaatcattt agtgctaact
     7741 gttattggaa gtatattatt tcttacttta gcatcattgg gctatgtagt tttaaaactc
     7801 aaataaatat aaaaacaagg ctgagaatca gccttgtttt cttttatttg aagattggat
     7861 tcgcactaat aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat
     7921 gacctaatcc gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat
     7981 ttttccatcc taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca
     8041 gcccagtttg acttatgttt tctcccttag caccgtagca gcctaagctt tccaataaat
     8101 cttggaaagt accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc
     8161 gaatatctgc agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt
     8221 ggcgataatc atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta
     8281 atgaaatctt tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc
     8341 gttggccgtt tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt
     8401 gatcagtcat gtttttccaa ggagtagcaa agctatcccc ttcgtatact gtttttgatt
     8461 cactagagag aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac
     8521 gagtgtagtt gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg
     8581 ttggttctac ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac
     8641 tgtcaccgtg agtgacattg actatctgtt ctttcaaaat attaactcgt ccttcgtttt
     8701 tgtagttgtt attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg
     8761 ttgtattacc tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat
     8821 tatctttagc gccttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag
     8881 aagttacttt tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa
     8941 tagtaaccgt atattctttt ggtgtttcag gtttgtgttg caacccttta aatgcagcat
     9001 caagactatt tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt
     9061 ttttagcatt ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt
     9121 tagcagttga ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa
     9181 ctggtttttt ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt
     9241 gcttttgagt cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga
     9301 atgcgttcca ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa
     9361 gagcttctag agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga
     9421 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
     9481 tgtagtgatg tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat
     9541 ttttttcggc agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc
     9601 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac
     9661 gatagacaag atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc
     9721 attttatctt tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat
     9781 tatttggatt gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa
     9841 gactaagtga ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca
     9901 ttttttcaac tcctaaattt aattaaaaat gtttacatga ttattatata tcggctaacc
     9961 gatatagtca ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt
    10021 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
    10081 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
    10141 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
    10201 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
    10261 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
    10321 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
    10381 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
    10441 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
    10501 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
    10561 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
    10621 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
    10681 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
    10741 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
    10801 gatgagaagc gaacgaaaga gaaattaatt catgatggat cagagttaaa taaatacaaa
    10861 ttacaaaaga ctagtgagtg attgctagtc tttttattta tcctgaagga ggaatcacag
    10921 tggcaaaagt taaaacacca ttcgaaaaag ccgcgtctaa aatcttagaa cgccgtggtg
    10981 tttctgaaat acagtggaaa aaagaagttc ttgaaaaagc acaatttgac ctattacgag
    11041 gaaaagataa ggacttagaa gaatatatct atcaacgtga acgtgaaaaa cttgtattcg
    11101 atgaaattat taaacattaa aaggagcgaa taatcttatg tttgaatcaa ttacaatgaa
    11161 ttttttaact caaccgaaag tagtagctgc agttgatatt tttagagcat tacaaactgc
    11221 cgcaactagt ttgaaaaata atctgatgat ttttgcggct gtagctgccg ttgcctccat
    11281 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
    11341 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
    11401 aatgttctca atgtttggac aaaccccaca aggactataa aaaaaacaag taggaggagt
    11461 aaaaagtacg tggctacttt ttactccttt ttttatgaaa ggagaatatt catgtttttt
    11521 acgatttcag gaaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
    11581 ggaaagcgta cttccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa
    11641 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
    11701 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
    11761 ccactggatt atcaaatttc agaaggtttt tatcctacat tgatagattt aatccgcctt
    11821 gatgtgttta aagatacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa
    11881 ctgctacaag ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt
    11941 gaagtcgaag taaatgaaga agtaccatgg gaacgaccaa tctttccaaa gaaggagtca
    12001 aaagaaaaag agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata
    12061 tttgaggaag agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg
    12121 gaggatgaag agacggacga agtagaagtt gatccagttg aagctaatga agagtcaaca
    12181 tggactgaag gggattttca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa
    12241 aacgaaacag agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca
    12301 gagcatgaat ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat
    12361 caattacccc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
    12421 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca
    12481 caagaagatt tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat
    12541 gagtatgcga cggaacagtc gtctaattgg gagaaagtaa aagaggaact aaataaagaa
    12601 gaacagcgag tgattgaaga agaagtacaa aaagtcaaag agcggtacga aaagaaacgc
    12661 gacaatgctt attccttgtg tgtagaaaag atcaacaatt ttactaagac gaatcaagaa
    12721 gcgttatcga aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagag
    12781 ttctaccgcg gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg
    12841 aataagctat atcaaacaac gactgaagtt cttatggaaa aacacgaaca gatccagcaa
    12901 gaattacaag tacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg
    12961 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
    13021 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
    13081 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
    13141 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
    13201 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
    13261 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    13321 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
    13381 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    13441 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa
    13501 ggatcaagcg attcaactgt acactcaaac acttccgaaa gttcagaatc aaatgaggtg
    13561 aaataatgcg tattagagaa acaacgagac gaaaagacaa acgattcaat ttagaaaaga
    13621 ataggtatct ttctcatttc tatcgctata ctccttcagg agaagagcta ccagaaaaac
    13681 atgacaaacg tatttctcaa aagatttttt atagtggctt agcttatctt cctttaaact
    13741 atgttgttac aagaatacca gaagcatttc atcgatttga gattattcca ggaagtttac
    13801 aaggagtgaa tatttttcag ttacaagaaa tgccgcagtt attccatttt gattcaactg
    13861 gtttttggaa tcctttgacc ccaacaacaa gtttaggcac acgtttagtt gtttctacga
    13921 tttgtgttgt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtccgctag
    13981 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    14041 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    14101 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    14161 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    14221 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    14281 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    14341 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    14401 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    14461 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    14521 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    14581 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    14641 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    14701 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    14761 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    14821 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    14881 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    14941 taggctataa aggatttagt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    15001 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    15061 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    15121 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggaaaaaagt aacttattag
    15181 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    15241 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    15301 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    15361 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    15421 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    15481 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    15541 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    15601 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    15661 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggagaa acgattgtac
    15721 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    15781 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    15841 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    15901 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    15961 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
    16021 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    16081 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    16141 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    16201 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    16261 gaaagtctta caagattatg ggaattatct gcagtatcaa gatgtattta gttggatttg
    16321 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    16381 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    16441 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    16501 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    16561 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
    16621 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggatcacc
    16681 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acgttactag caactgaccc
    16741 gaataaagta gaatccctac caacagataa aagaaatgct ttaacagcga aaaattttaa
    16801 aacagcgaat atcaatggga ttattactcc tgaacaagct aaaggtatgg caggtaatga
    16861 taaaagtggg ttatcagata tacaaaagga tcggttaaag gcattacaat accgattgga
    16921 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    16981 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattattt tagctggtga
    17041 atcttctttg gcagtagcct atttatttat cctatttacg atcgttactt gcattattga
    17101 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggtca
    17161 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    17221 tttagaatta cgaatctata ccttaatgct ttcaggtatc ggtgatttgc atgcggcagg
    17281 aaagatcaat ggattcttgt atgtcgttgc tttgattgta ctaaccattg cgctattcaa
    17341 aggctctcaa tcagttacga aaattttcgg cgttgataca agcctgaaaa acggcagtaa
    17401 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    17461 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    17521 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    17581 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    17641 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    17701 tgaagatctt acagaaaaag ccaaagacac cttagaagat aaattagacg gcacgagagc
    17761 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    17821 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc
    17881 agtgaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    17941 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    18001 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    18061 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    18121 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    18181 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    18241 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    18301 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    18361 agatagtcgt ttgaatcaat tcaaggataa tgatcaaagt tttgttcatc aatcacctgc
    18421 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    18481 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    18541 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    18601 atttactgca ccaacaaaaa taaatgtgac aagagaaaca aaaaacagga acaatgcatt
    18661 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    18721 aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata gtaacatgaa
    18781 aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt tagatctatt
    18841 aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc acttaccact
    18901 cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga ttgtttggcg
    18961 accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta ttttttttgc
    19021 ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    19081 ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca ttttgcagtt
    19141 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
    19201 gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag tttctacaga
    19261 cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    19321 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    19381 acaaatcgtg caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag
    19441 aaatctacaa cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa
    19501 acgagcagaa caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat
    19561 caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt tagggttaaa
    19621 accactctac aaaaaagaat tgatcaagat tctgtatcga atgaacaata tgaatgatga
    19681 gtaggaatgg agaagagaga atgaaatatc cgttgatatg gttagaaaaa gatgttccga
    19741 aggaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg ggaaaagggc
    19801 aatcttttaa ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt tagaagcaat
    19861 taaaacagct ttttcaccac aaaatgatca aaaagtactt gatccattaa agcaactagg
    19921 aaatcgggaa atagaaattg taaaatccaa agggtatgat ttagattttt taaaacaagt
    19981 gatgccacaa ggtgggttaa attttgatga agaatatgtg acgtatggta atggttattc
    20041 acgatgtgtg acagtttatc actacacaaa aaatcctacg cttttttggt tggccaatct
    20101 catgaacaat cctcatacag tggccacact cgatgttagc acagatgaaa agcaaaaagt
    20161 gattgatcaa atcaatcgtg ccttagatga attaaatgat cgatcagata atgaacgaaa
    20221 agcgactgac cgaaataaaa gcgcggaaga atacctggaa ctagcggaat atgctagaaa
    20281 attgacttct aatggggaaa tttctaagca agtcaaagtg agagtacatg tttacggaaa
    20341 cacgattcaa gaagtagatg aacgagcgca ggaactgatc gaaaatctca aagggatgga
    20401 ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat
    20461 gttcgatgaa caagctgaat ggttcggaaa tatcaaaggg caatcactac catcgttaaa
    20521 cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggag ggacacaata
    20581 tggggaaacc atgacaggcg gcccatttgt ctggaatgcg acgtataaaa atagtgtacg
    20641 gttgtctact aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa
    20701 aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa
    20761 agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga
    20821 cggaatgatc aatcctttag agatatttgc cacacggacg tatgatgaag catccagttt
    20881 atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca
    20941 cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa
    21001 agcagaattt aagacctact taaatcgttt ttatgaagag tatggaacag taccacgcgg
    21061 ctttacttcc tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga aagatccaga
    21121 agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat taccaggtgc
    21181 ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat ccttatgtga
    21241 gacgtatggc atgatttttg atggtcatag tacgattcga caccttgacc aacaacagtt
    21301 ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac aatgtcaaat
    21361 gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa gacaacgacg
    21421 tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga
    21481 tgagtgccat aatggtgcga cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta
    21541 aaaactattg taataactct tataacgaga agtcaaatga gtgggtaacg ccacaaaggg
    21601 cttccactga tactccgaca tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct
    21661 cggtgaagtc ggctgaagga taccgtaagg taaaagaaat agtgaatacc acgaaagttc
    21721 tctagaggta aagggtgtat ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt
    21781 atgtacaaac gtactgacga actagcgaat gtactgtccg aaaggcatag ctcctagaaa
    21841 tgggagagac agtgagcaaa aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg
    21901 ttgttatgaa agaccatgct gcgttacagg cgtaggagaa gttagggggc aaaagctagc
    21961 atctaagtta aggcatgtgg atagatataa gggaacaggg gaagccatca acatggagag
    22021 tgcatactcg atgaagtggt agtaagaaat atgtgaatga caaataactt actttagtga
    22081 tggtgaaagt agagtcacga ctgatgaagg ttctgtaatg gaactggagg aacaggctcg
    22141 attcgtctgt aaaagtcatg aaacccgttc aaccatggga ttctcgtatg actaaaaagg
    22201 tcacgagcca attggagcgt gataaaccat gacaaaagaa acaagtccaa aagataggaa
    22261 gttgcggcat gcagaatatt atggcatgac ggaaatcttc gataagatat actccaaaag
    22321 tttgaaggga gataatttca agaatctaat gcagattatc atttctgata acaacattgt
    22381 cctagcatat cgtaatatca agagaaatca cggtagtgtg acaagaggag aagatggagt
    22441 aacgattaaa gatattgaaa atctcaaccg taatgaattc gtagaaattg ttaggaagcg
    22501 attcgcacat tataaccctc ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa
    22561 attaagaccg ttgggtattc catcaatatg ggatagaatc gcgcagcaat gcatcttaca
    22621 agtattagaa ccaatatgtg aagcaaaatt caataatcac agctatggtt tccgcccaaa
    22681 tagatcagca gaacacgcaa tagcagattg tatggcccga atgaatcaaa gtcacatgga
    22741 atatgtggta gatgtagata tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat
    22801 gagacaaatc tggtctatgg gtatacgaga caaacagctt ctcttaatca ttaggaaaat
    22861 gttaagagca cccgtagtac ttccaaatgg gaagttgata tacccaacaa aaggaacacc
    22921 acaaggcgga atacttagcc cattacttgc gaacatcaat ctcaacgaat ttgactggtg
    22981 gatagcaaat cagtgggagg aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa
    23041 tggagcttgg gataaaggta atatctatca caagctcaga aagagtacaa ctctaaaaga
    23101 aatataccta gtgagatacg cagatgattt taaaattttc tgtagaaatt ataatgaagc
    23161 gaaaaaagtt ttctatgcaa ctaaaatgtg gttacaggag cgtctaaagt taccaatatc
    23221 tgaagaaaag tcaaaaatca caaatctttc aaaagaagaa agtgaatttt taggctttac
    23281 gatgaaatta gtgaccaaag gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc
    23341 acaaagcatg attaaagtga aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa
    23401 tagcaaagat acctttatct atattggagt ctataacagt atggttattg ggattcataa
    23461 ttacttcaaa attgcgactc attgtagtgt agcaatgcga aaaattgcaa gagaactcag
    23521 accaagtttt gaaatacgac tacgagaaca aggaatctca aaagaaggac aatacggaaa
    23581 gtacgaagga tacaagcctt acctacggtc aggacaaata cgatatataa gagggtttcc
    23641 aatattacca gtagggtata ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa
    23701 taaatacacc gaagaaggta gggaagccat tcataagaag caacaaaccg tagaagaatg
    23761 gaagatatcg tggttgtgta gcaatccagt gcttagtaag agagcaacga ttgaatttgc
    23821 ggacaatcga atttcaaaat tcatcgctca aaagggaaga tgtgcagtaa caggtgagga
    23881 gcttatctta tctgaaatgc attgtcatca tattatccct tatcatgaaa gcaaaagtga
    23941 tagctatgaa aacctagtga ttgttactga ggaggttcat cgggtaatac acgctacaca
    24001 aagtgaaacg attgaggaac ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa
    24061 actcaatgag ttgagattaa aagttggtaa cgaagaaata tcttaataaa tatttgtagg
    24121 acgggaaaac agaatgtctt ttatagacga gggaacgccg tatgcggtga aagtcgcacg
    24181 tacggtgtgg actgggggaa aagacggaga taatatcaaa ctcttaccta tcagtatcga
    24241 ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga gaaatgcgta
    24301 aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata
    24361 tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct
    24421 ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt aaatcaatga
    24481 cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaattttg
    24541 gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta aaattgtttg
    24601 atggaggaaa ataatggaaa accaaaatga aagtttaatc aagcagtatg ttaagcgtcg
    24661 tgcaaagcgg cgcttatttt tgtggttgtt tggtacttct gcaggtttga ttactatttt
    24721 gatcactgtg ttcgtgaccc tatttctaat acttgcggcg ggctcgattg ataattcgga
    24781 ttcagattca agcagtggtg gcgaagcttt tacaggagaa tattcggaag gcttaccgat
    24841 ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgccgtagg
    24901 tgcagcagtg aaatataaat tattaccaag tgtgatcctt tcgcaatacg gttacgaatc
    24961 agcgtttggt acatcggcgt ccgcaagaaa tgatctcaac tattttggga ttacttggtt
    25021 tgatggttgt ctgtttccta aaggaactgc acgaggaatt ggtggcattg aaggtggctg
    25081 gtacatgaag tttcccaact caaaagctgc attttcctat tacggcttta tggtagccac
    25141 acaatccaat tttaatgctt gtgtaggaaa taaaagccct ggcgcaagct tattgatctt
    25201 aggacgaggc ggttatgccg ctgcaggaat tacggaagat agtccgtatt atacaggttg
    25261 tatgtcaatt atcacttcta ataaattaac cgaatatgat gaattcgcca tcaaacattg
    25321 gggtgaaggt ggcaataaca acggcacgat tactggtgaa tggacgaatc cattccctgg
    25381 cagcagttta gataaaagtt ccttcagcgg tggtcaatta tttggaacga atccaggcgg
    25441 tgaattccgt ccgaacggct tccatgatgg attagatttt ggttcagttg atcatccagg
    25501 aagtgagatt catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc
    25561 aggtttgggt gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga
    25621 atttgcaaat agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca
    25681 agtaattggg atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg
    25741 tcaagcacaa ggacatgcat tcattgatga tggtacatgg atcgatccgt taccattttt
    25801 gaatagcagt aaaaaataga tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt
    25861 cttaacagct gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa
    25921 agaggaacaa gcagcaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa
    25981 gctcaaaaaa aattcctctg aggcacaggc gaagatcgac aagttaaatc aaaccatcaa
    26041 tgagaaaaac caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga
    26101 agtaactgaa gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc
    26161 attaagcaga aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga
    26221 caaattaagt cctacagacg atgatcacca acatgatgat tacggaagtt actcctttac
    26281 attaggtgac gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt
    26341 cgtatttgtt gattatacaa tcggcaatcc gcaattcaaa gatgtcaaac cacaaaaaat
    26401 aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcatagactt
    26461 tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagaa agacaacgtt
    26521 tcaaaaaacg aaaaagttat cttagatggc tgggaatctt tatgattctc ggctttttga
    26581 ttcttgctag tccgatttat acgaatcaga aggtagatat accagatgag gtaagcacga
    26641 acgccttttt aaacacgaaa gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc
    26701 aattagtttt tgaatttgcg atcgatgatg aagatcaaag cttgttaaaa gaactggcta
    26761 atcttaatta tcgcgtagaa gtaaagacag ctaaagggga ttatcaagca atacgagcca
    26821 aagtgattaa ggtgtctgat gattattttg cagtgaagct aacgcatgta ccagaagaat
    26881 atatcgccat gcgtttaacc attattcctg aaaaaattga tccaaaagtg gatatgcaag
    26941 aaccacaaga tctgattttt tatattcacg aagataaggt caaagatcac gtgaaagacg
    27001 aagattatga acaacatgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag
    27061 cagctcaaaa acaaatggaa tctttccaag caaccattga tttgaatgaa gaattagtga
    27121 agaagctaaa agatcaattg ccttatcaag tcgcagatga tcaggaaaat actgaaaaca
    27181 agattaatag ctaccagcaa gaaattgaaa catcgaaaac gcaactgaaa gatcaagaag
    27241 agatcattca aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaag atttagaagg
    27301 acaggtggag tgaaaaaatg aagcgaacaa acttggctga caaagatact ttcgctgagc
    27361 agaaagtatt gattaaagcc tatctcaaag ttgtaaaagc taaagaaata tgtcgagaag
    27421 tgaatttatc taacacaaca atatctgagt tgaagaatgg tcgaaagaat ttagatacct
    27481 taaaattcag atcatttgaa gctctatatg tagctgctaa aaaatatttt acagctgata
    27541 acaaataaaa aacaggagta ttttatgtga aaaaagtata taaaaaatgc ttatgaatca
    27601 gctatatcac cttaaccatt tgttaatgag atgggagaaa acattaacca cgattctaac
    27661 gcaaacatca agcacataaa acatttaaca ctcctccttt ctggctgaag atttcaacca
    27721 aacaggaaga cagcgtatca attatagcat acactttttc ttaaattcat ttttcatatg
    27781 acaaaatatt tccttctaat cgacaaagtt agaaggattt tttattgtct aaatacacaa
    27841 ataaccttaa tttttaaaga gcataaggaa acgtgatatc actttctcac gaaaggtgtt
    27901 aggtaggctg tgcctaccac gtttctaaga aagataaatg atatcaaaat tttggatttg
    27961 tgtgtagatt tgtgggtagg atgaaagcga atgtgggcag taaaataagc gtttgtgtgt
    28021 agcaaaatca aaaaatgtgt agggatatgt gtagctacac atatccctac acataataaa
    28081 tttggaggaa aatatggact tattaattag aaatattgaa gaaaagtata ttaataaaat
    28141 tgacaaacgt tgtgaagaac ttacggtcaa gactggcaaa aaatggagtc gaaatcaata
    28201 cctaaaagtt ttgatcgaaa atgattttga tcacgctctt ctgaattata aaaaagacca
    28261 atttgatcgg ttgttagaaa aatttgtgga tattcaaact tataatacga aaactttaga
    28321 agaatatatt gcaacaaata atcaattaat tggtttgtta attgaataat aaaaatagta
    28381 ttgtgaattt ataaaaggag gaaatcaatt ttggcaaaag caacaagaaa acatgtatat
    28441 ctttacgata tgcatatcga agtacttgat gaaataattt ctactcatga agatatatct
    28501 aattatagtg aagcagtcag acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa
    28561 ataaatccaa ttcaaattaa attgaatgca atgggaaaag aaatttctat tttaaccgaa
    28621 atggtttcag agtttgcgga tcttcatttg aaagatacag gaatactaat tggtacaaat
    28681 tcaactgttt atcaagaagc agaagaacgt gtggaaggaa aaattaaatc caatcaaacc
    28741 aaaaaatatt cttctaagtt aaagaaacca gtgaatgaat ctgttcatac cagagtagat
    28801 ccatttgatc caacgaagat tctaaaaaga taaaagtaga aggagaacgt gatgaataaa
    28861 tcgcctgcag ttattcttac ttgtcaattt accttgccca ataaaaaatc tttttcaaca
    28921 tacattgatt atatgacaag agaaaaagca ctggaagaaa tggaacgacg tacgcccgaa
    28981 gaagaacagg aactgcaacg aatcaagaat gctttagagg atttttatat tcctgaagga
    29041 gaaacttttt cggaaagtaa gacggataaa gaattcagtg aaacgtcgaa tgaagctcaa
    29101 tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt taccaaatat
    29161 ctttcttata tgacccgtca ctatgcgtta gaacaaaaga aagaactgac gcaaagggaa
    29221 caaaaagaat accaacggtt gaaccaagca atgaaaaaat atcaaacaga gccacataaa
    29281 aaagcttcag gaaatcttcc tggagttttt tctatggctt cagacgaagt gaaaggaagt
    29341 gatctgaagg agatcaaaca aatcttccaa agaggacaac aaaaaggctc aatcatttat
    29401 caagatgtta tctctcacga caattcgtat cttgaaaaaa tgggcttgta cgatcccaaa
    29461 acagatacct tagatgaaga aggtttgaaa gcagctggaa gaaaaatgat ggagacatta
    29521 ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca ccgtaacact
    29581 aagcatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa accgtatact
    29641 gagattgaaa atggtgtgag ttatatcgta ccgaagggga agagaagaca agcaacatta
    29701 gacgcaatga aaaccaatta tgtacatgag ttagaacgtt tctcttatga aaaggatcga
    29761 atgatcggta tggagaaacg aaatttattg acacgaaaaa gtgatttgcg taacacctta
    29821 acaaaagtag taaaagaacc agcacgttac gatcgacacg ccatgaactt attaaatgaa
    29881 gtatataaaa ccctacctga aaaacgttcg gaatggaatt atggagatga aaatcgaacc
    29941 aagctttcac catttacaag gggaaaactt gatgagttaa ccaaatatgt tttaacaaaa
    30001 gaacctggat atcaagaata tatacagatt tcaaaagcat tgaaagaaga agccaaaagc
    30061 ctttatgggg aaagcaaaag agaaacaaag aatgcagaaa aaaacgccct ctttgattta
    30121 aaaaagcgca caggaaatgc gattttaaca gaactgaaga gacaggatag tcaaataaaa
    30181 ccattaatag aaagtgtcaa tcagatgcaa tctatttaca atgaacgatc ctttcgaaca
    30241 ctcaaacagt tgccaacaaa tgaatacaag tatagttatc aagaattcgt aaagcgtagg
    30301 aaacaattcc aacaaagagt tgtcacgcag cgaagcttgc gtcaattaaa tcgaacaatt
    30361 aatgcacatg aagaaaaata ccacgcaagt aaagcatatg aagagcaaca gcgaagaatc
    30421 caaatggaac aagaaagaca ataggaggaa gagaaatgaa acaatattta gatcaatgga
    30481 aagtcataga aggatcacta aaagaagaac gaatcgaaca acttcctgat tgtttagaaa
    30541 aagagcattt gtttcagata agcgaaatgt tgagaaacga acagtttgat ccaaatcaat
    30601 ttttagttgt tgagtaccca gctactggtg tgtattgttg taatcatgtg aagggtgaaa
    30661 aatattttat tattcaagaa tacgaaggta agttagcgcc ttattatact acttgggaaa
    30721 taaatgaaga gggaatcaat aatttcccat gtgaaagcat tgaagagtct atttctctca
    30781 cggaatgtta aataaataga gacgtaaaag agtcctgaac gtgattgtcc aggactcttt
    30841 ttcaatgaat tgttttccgc caattcactg agtggtgcga ttcaatttta ttatatcaaa
    30901 agaaagtgag attccaaagt gaagggagga agaagaaatg actcagacaa cgattggtgt
    30961 tgtggttgaa aaaagcaggg ataatctcgt ttttgtgaca gaaattcaaa caggtagagc
    31021 ttttgtagtc acagacaaag ctgcaaaagc gtatcaaaat ggcaatattt taacgttaaa
    31081 tctgactacg aagatgttcg ttgatgcagt agaaaattat ccatttgttt agacaaacga
    31141 tattttacaa ggaggaaacg gacatggcag aagaaaaaga accactagat aatagtcggt
    31201 tgggaaagtc caaaagaaaa ttagtacggt tgcaaaacga attaaataat caatttcaaa
    31261 agctatttga tcaccaaaaa ttgacaaatg ggcaaccaat gaatgacaaa cgaaatggtc
    31321 gttcatggtt taaccagcaa gaaaaaatag aaaataaaat acaaagtttg agagaagaga
    31381 tcaagcaaca agaaaaacag gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg
    31441 gctataacaa gtacggcgga ctggatatga cgatcgataa tattccaaga atcaaagaag
    31501 aaatcgaacg ctttgaaaaa ggtgaatcga ctttttcagc cgccacgatc cgaaaatatc
    31561 aacggaagtt ggaaacatta gaacagctga aagaaaaatc agaaaaagga aaagaaaata
    31621 tcctccctga agtacaagcg atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc
    31681 caaccattta ttttttgaaa ggctatcgaa aagttgcttt agaactgaat gaaaacgggg
    31741 gatttgagga atcaaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg
    31801 aattacttga agaggctgca ccaactgatt ctctgaagga gaaagaaaaa gatcatttct
    31861 atcgagtaga attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga
    31921 tcgtaacatt agaattgatc gatcaactta aaacgttgga taaccaatac gctgcagaag
    31981 caggttatta taaattctat tttgaagaag tgaagaacgg agaagtagta gatcagatcc
    32041 gaatggatct aggggatggc gtcaacagta atcaccccat ctatttagag ttagcaaaat
    32101 tgtgcgaaca agtacctgag aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga
    32161 aagaacaaat gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg
    32221 cagaagagat caaacaagaa gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa
    32281 agcataccca ttcttctgaa gacgtcaaag aacttttgaa ctttatgtct cgttttccag
    32341 aacgatcctt tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt
    32401 taggtcgtgc cgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca
    32461 agatctttgt cagaaaaacg gtcaaaggat tttatgagaa aggacgtggc tttgttcgag
    32521 aaacagaagc tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca
    32581 aaaaacctta ctacacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg
    32641 aagactatcc gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac
    32701 agtcagaact tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag
    32761 atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacgtggg gcgtatgttc
    32821 gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc
    32881 atttagctac aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag
    32941 ataccgcaac taaagaattg caggcagaaa tgacttctta tattgtgtgc aaacattttg
    33001 gaatggacac ttcggagaaa gctattcctt atattgcagc atggacgaag aatggtcaga
    33061 cgttggatga taaggaagcg gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg
    33121 caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa agagaaccag
    33181 agatccaacc agcagaagtg aagcaggagc ataaaaatac tgtagaaacg gcagagaata
    33241 agaatattgt gcccgttggt tcactttgga tcgacaagaa agacgggaaa gtaatggaag
    33301 aagatacggg tcgaacgtta catttgaagg atgcggcgtt tattgaagat ccgcagcata
    33361 atcaagtgat tttaaaagga aaaagttatg gaaaagagat tcaaacggcg ttaccttcag
    33421 aagaatttgc gctaggaaat ccattccgat tttcacttga aacagtgtca gaagtggcca
    33481 aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgc gtataaaaca
    33541 agtaaaaaag actatctgaa aaacggatag tcttttttct tttgggaggt ttgaaaatga
    33601 ccgaaaaaaa aatgagtctt attgatcgat gtaaacagat cgatatagtg gattttgctc
    33661 gaaacaatgg aatggctgta gtcaataaag gacgagacta tcgcttagaa gaccatgatt
    33721 catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat attagtggcg
    33781 acattattga attagctaaa ctttttttca tagacaaaga gatccaagac tctaagcaac
    33841 aatttaaagc agccactgat tttattctaa agaatgaaga caaaacagaa cgagtggaaa
    33901 atcttcattt tgaaacagaa aagtacaaag atcaccccgt tgattatcaa ccactcacag
    33961 aaaaaggaag gaattatctg aaggaagaac gaaaactacc agagtggctg attgattatg
    34021 cagaaaaaga agggttgatt gccgaactaa agccaaaata tgaacggcaa aatttcttag
    34081 ttcgagacga tcgtttagat catgcggtcg cctttttatg gaaagatcca cagacaaaag
    34141 agacagtagg ggcgagttat caaggaacgt tcattgatta tgaacgtttt ggtgagagag
    34201 gcacatataa gcatatagat aaaaattcga cagcgaatca tggctttaac ctaaagatcg
    34261 gtgaccctaa gcagttaaaa ttttttgaga gtagtattga tttacttagc tatgctgcac
    34321 tgaatcgtga tcagttaaac gatacgtggt tagtctctat ggaagggtta aaacaccatg
    34381 tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
    34441 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
    34501 aattaatggg cgcagtcgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
    34561 ctaatgattg gcaagtgcca aaagaataca aaatcattta tgaagaagtc gcaaaagaag
    34621 aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
    34681 cgaaccaact ggtttccgct cacaaagtaa atgcgttttt tggccagcag ctttctgtta
    34741 acgattcaat agaagccatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
    34801 aagtttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
    34861 atattaatgc gcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
    34921 atcatgaaca tgaatttata cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg
    34981 agatccagca acaagaaatc agaaagcaaa aacgtgccat gttgtttcaa caaggcagac
    35041 aacaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca
    35101 caagcgaaag cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag
    35161 atccaagatc gattgtttac aggagaaact gtgatcacgt atggttttgg tcatttagtc
    35221 gagttagatt cgcccgatat gtacgatgaa aaatggaaac aatgggcatt agaacatttg
    35281 cctattttcc ccaatcaata ccactatcat gttcctaaag ataagaaaaa gcagtttagt
    35341 gtcgtcaaac gacagctgca gtcagctgat acgattgtga tcgcgactga tagcgatcgt
    35401 gaaggggagc tgattgcctg gtcaatcatt caacaagcag gcgccaatca aggaaaaacc
    35461 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccaacag
    35521 ttacgagacg cagaagagac ctatccaaaa tttgaagaag tacaagcacg tcagatcgcc
    35581 gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc
    35641 ataccaggaa gtttttcttt aggtagagtc caaaccccaa cactctatat gatttatcaa
    35701 ttgcaggaaa agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta
    35761 atcgctcaaa atggcgcatt tgatgcgaag cttgatccaa atgaaaccca agcgacacaa
    35821 gaagcatttg aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg
    35881 atccttcaag tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg
    35941 agccttcagt ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac tttggaagcc
    36001 atgcaagggt tgtatgaagg aaaatatcta agctatccgc gaacggatac cccttatatt
    36061 acggaagggg aatatgcgta tctgttggat cacttagacg aatacaagca ttttttaaaa
    36121 gccgaagcga ttccgactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa
    36181 gtccaggaac attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa
    36241 caactaagtc cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg
    36301 tttgcggaaa agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag
    36361 ttaaaggcaa tcgaaaaggt gccgctagat ctaggttgga agaagttatt tggaaaagag
    36421 agcgacggaa aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg
    36481 gtgacggtgg atctacaagt tcttgaaaaa gaaaccaagc caccacagcc ttataccgaa
    36541 ggtacgttga tcacggcgat gaaaacagct gggaaaaccg ttgatagtga agaagcgcaa
    36601 tccatattga aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa
    36661 accttgaaac aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa
    36721 gggattttgc tttgccaagc cgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc
    36781 gccaaatggg aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt
    36841 ctagccaata tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa
    36901 tccactgatt ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc
    36961 gcgcgtcatt taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag
    37021 gtggcagcat gtacgtctga agcctgtgat ttcaaactat ggacaacgat tgccaaaaag
    37081 aaacttacag cgacacagct gaaagagatc attcaaaata gccgaacaag ccaaccagtg
    37141 aaagggttaa aaggtcagaa gggttcgttt gaagcaacga ttgttttaaa agaagacttc
    37201 actacaggct ttgaattttc tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc
    37261 actacaaaat aatggagggt taatcttatg cgtaatacaa cacacaaaca acaagaaatc
    37321 gaaaatcaaa aagccaaaat tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga
    37381 ctgcagcaat tgaagaacca gcagcggatt caaaaagaaa tcgaattaga tcgataaatt
    37441 cctttcttgt taatataaaa ctaacatgtt atagttgtgt tagttttata ttaactttag
    37501 gggaagagta atgaaagaca aaatccaatg tatttttgat aaacataatg gcaacctatc
    37561 cttgaaggat gctagaagtg aaggattatc gcctatgacg ttagatcgat tagccgaaaa
    37621 ggataaaata gaaaaggtag caccaggttt ctatacctta aaggatgcgt tcgtggatga
    37681 attgtatctc gctcagagta tctattccag agggattttt tctcatgaga ctgcgctcga
    37741 tctttttcaa gtaggaacat atatccctaa aaagatcaat atgatgtttc ctaaaggata
    37801 caatgtatca aaggagaaat tagatcagta tgccattcaa ccgcattaca ctaataaaga
    37861 aaattttgac ctgggagtca cagaaacaga aagcttttac ggcaataaaa ttttagtcta
    37921 tgatttagag cgaacgttat gtgacatgtg gaatccaaga tacaaagcaa gcgtagaggt
    37981 aaaacaagaa gcattaaaag aatatatgac aagtaccagt agaaatacaa ataagctaag
    38041 aagatacatg gatattttaa aatcaccaaa agaaatgact ttgtatatgt tgccacttta
    38101 ttgagaggag taaagatgaa tgacaccgac acaatttaaa gagaaaacaa gaaatttcgc
    38161 agcagagaaa catttaaacc cacaaattgt ccaaagatat tatatgatgg aaaaattatt
    38221 agaatgtatt gttgaatcgc cttatcatga tgattttgta ttaaaagggg gatttctgat
    38281 tggatcaaaa tacgggttag aaaaccgttc aaccgttgat attgatacga cttatcgaaa
    38341 ttcgaaacta acaaaagaaa agttagaaac tgttttttct gaattaacag ctaatcctac
    38401 aaaagatgga attcgttttg aattaaaggg attaacagag acgagggaag ccgattatta
    38461 tccaggtttt caagcaaagt ttattgctta tcttgaaaat gcacgtgtac cttttaaatt
    38521 agatattact tcaggtgatt ctattattcc agcagcattg atgtatgaac acaaattaat
    38581 gtttgaagat aaaacgattc atattccagc ttatcctacg gaacaaattc tagctgaaaa
    38641 attacatgcc acgtttagtt ttggaaaaga taattcgcgc atgaaagatt tttatgacct
    38701 gtatatgatt ccgaaattag aacgtatcga tgagaaaaat ttgtataaga gtgttcaagg
    38761 aacattcaag gaaagaaata atcaatcggt gtttaagcat ttgtatgatc aggaaatgcc
    38821 tcaattggtg aaagatgagg gcttgagaga aaagtggaaa agataccaac aagaaaacta
    38881 ttttgccaaa gacatttctt tcgaatctac actaacttcc gttgattccc tgatgcaatc
    38941 aattgtagag caagagaagg cagaacgaaa acaaaatttt aagagatcac aacaaatgaa
    39001 tcaagaaatg gagagataaa gatgatcaat aatgtaactt tagtagggcg attaactaaa
    39061 gatccagatt tacgttatac cgcaagtggc acagcagtgg cgactttttc cctagccgtt
    39121 aatcgtaatt tcacgaatca aaacggcaat cgagaagctg attttatcaa ttgcgtgatt
    39181 tggcgcaaac cagcagaaac gatggcgaca ttagctaaaa aaggcagctt gatcggcgtt
    39241 gtcggacgaa ttcaaacacg gacttatgac aaccagcaag gacaacgtgt gtatgtgaca
    39301 gaagttgtgg ccgataactt ccaactatta gaaagtaaag cagcgacaga aagccgtatg
    39361 cacgctgatc aatcgtccag cagtccaagc cctaccacct ttgaacaacg ggatacagcg
    39421 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
    39481 attagcgatg atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt
    39541 ttatgttata atttagacaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg
    39601 gcgggctagt ggctttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc
    39661 tttcttggca aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat
    39721 gattgcaaaa acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat
    39781 taggccgggg tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg
    39841 atttttgcta taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata
    39901 aaatccgagt aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg
    39961 aatcgattaa ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg
    40021 atggaaaata caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg
    40081 ctagcatgta tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaataaaag
    40141 aagagttgta gtcctaaatg aggattgcag ctctttttgt tttacataga aacaggaaaa
    40201 gaccttttta ggaagacaca agtgccttct caattgaaaa acctttctct cactccaacc
    40261 gccaccgccg ttcgagaaga aaggaagtga acgctgcacg ttcactaaga tatggtgtct
    40321 tgcaaagcaa gacaaaatag acaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
    40381 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
    40441 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgatttattc
    40501 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
    40561 ggcagaatca aacatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
    40621 cccaaactat catgcaccac atactgttga atgcgattgt ggagaaagag cgaaatgtat
    40681 caaaagctat cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg
    40741 cgattacatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acacaaatta
    40801 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
    40861 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
    40921 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
    40981 aacatggaaa cttatgaaat cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
    41041 aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac
    41101 aaaaaatgga ggatatgacg ttcctatacc atgaaacacg tgtacccaaa aaacattaca
    41161 aggaaaaatt gtcagtcgca gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca
    41221 ttgcgacgta ctatcgaaca ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc
    41281 aagccttact atgcttggaa gtaggtgtca aaccaagcac tatcaaacct tccgaatatc
    41341 aagccttaga actgacttat gcaaaattcg cacagattaa gaaagctaaa gcaatggcta
    41401 gcgactggct agatactttt gaaagtatcg aaaaaaatgg tgcattttat acgattgaat
    41461 cggaggagaa agataatgaa taaggtggtt tatcagctaa caacagaaga tgtttcgcaa
    41521 tttttaacaa gcaaagaaca cttgaatgaa gtaatcagac ggattcaaaa agactttagt
    41581 tgtatggaag aaatgaagta ctttattcaa agagtggaag aacagttgta tatggaaagc
    41641 gaaacaagtc aaacgaaggt cacagtcagc cataatcatt tagacgtgta taaacgtcgt
    41701 cctgaaacgg taaaaaaagg aacagagctt ctacgactag aaacagaaaa atacatcatg
    41761 aatgtgtacc tagaaaggag tgagctggtc gaaaactttg tcagctacac gatttgtagg
    41821 aaacactatt actcagaatc agaatattat tcaattttcg agagcatgga attaatggaa
    41881 gaagaaatgg aagagctaaa cagcaaaaat gcgacggtag acgattatta ccttgtattt
    41941 actaggtttt tgacgtgtgg gacggaatct atcgtgttaa tgggaatgga ttatgaactc
    42001 gctgtacccg aagcattttt gagggaatat gtagaagaag tagataaccc aggcgacgac
    42061 tggtacacga ctttaactga ttttttagac acctacactt acgacgatac agaagaaatc
    42121 caaaatgaac ttgaccgtaa acgaatgcca tatcaacgat ttgaaaggaa aaaaggtaaa
    42181 taaccattta taccgaaaag tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa
    42241 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    42301 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    42361 ttcctctatt gttcgtactg tttgttggcg agatacttat gcgtcatgct gcagctgtac
    42421 atttaaactt cgtagtattc ttaggaatac tggtattttg tttgatcttt tggcgatttg
    42481 tattagccct tgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat
    42541 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    42601 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    42661 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    42721 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    42781 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt gggagcgtca
    42841 ataattttgt gtaaataaat tgtcctcctg caaaataatt agttactcag taaacattga
    42901 aactaatgta tcggttacct gttgaaaacc tttatggctt ctgtttagaa atttttgatt
    42961 gtatgtatca aaaatgctga ctagaaagcg ttctagtgat tcttcatttt gaaactgctc
    43021 ttttctacgg ctgtatcttt taatttgctt attgaaagac tcgattagat tggttgagta
    43081 aatggttcta cgaatgctag gtggaaaatc ataaaaagtt aataagtctt ggttttctat
    43141 gagtgactgc gtcactttag gatagttttt cttccatttc tcaatcatgc cggataagaa
    43201 ggtattcgct tcttcttttg agttagcttg ataaacagcc ttaaagtcat cacagatttc
    43261 ttttcggtct tt
//
LOCUS       DASJUF010000011        40624 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJUF010000011 DASJUF010000000
VERSION     DASJUF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.011"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(609..2105)
                     /gene="lysS"
                     /locus_tag="U4B62_000542"
     CDS             complement(609..2105)
                     /gene="lysS"
                     /locus_tag="U4B62_000542"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN2140724.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2216..3220)
                     /gene="dusB"
                     /locus_tag="U4B62_000543"
     CDS             complement(2216..3220)
                     /gene="dusB"
                     /locus_tag="U4B62_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN2140725.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3221..4108)
                     /gene="hslO"
                     /locus_tag="U4B62_000544"
     CDS             complement(3221..4108)
                     /gene="hslO"
                     /locus_tag="U4B62_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN2140726.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4325..6436)
                     /gene="ftsH"
                     /locus_tag="U4B62_000545"
     CDS             complement(4325..6436)
                     /gene="ftsH"
                     /locus_tag="U4B62_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN2140727.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6526..7071)
                     /gene="hpt"
                     /locus_tag="U4B62_000546"
     CDS             complement(6526..7071)
                     /gene="hpt"
                     /locus_tag="U4B62_000546"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN2140728.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7071..8516)
                     /gene="tilS"
                     /locus_tag="U4B62_000547"
     CDS             complement(7071..8516)
                     /gene="tilS"
                     /locus_tag="U4B62_000547"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN2140729.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8636..9106)
                     /locus_tag="U4B62_000548"
     CDS             complement(8636..9106)
                     /locus_tag="U4B62_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN2140730.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9152..9577)
                     /locus_tag="U4B62_000549"
     CDS             complement(9152..9577)
                     /locus_tag="U4B62_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN2140731.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9644..9913)
                     /locus_tag="U4B62_000550"
     CDS             complement(9644..9913)
                     /locus_tag="U4B62_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN2140732.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9924..11519)
                     /locus_tag="U4B62_000551"
     CDS             complement(9924..11519)
                     /locus_tag="U4B62_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2140733.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11534..15055)
                     /gene="mfd"
                     /locus_tag="U4B62_000552"
     CDS             complement(11534..15055)
                     /gene="mfd"
                     /locus_tag="U4B62_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN2140734.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15072..15632)
                     /locus_tag="U4B62_000553"
     CDS             complement(15072..15632)
                     /locus_tag="U4B62_000553"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN2140735.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15985..16959
                     /locus_tag="U4B62_000554"
     CDS             15985..16959
                     /locus_tag="U4B62_000554"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2140736.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17129..18529
                     /locus_tag="U4B62_000555"
     CDS             17129..18529
                     /locus_tag="U4B62_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN2140737.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18607..19329)
                     /locus_tag="U4B62_000556"
     CDS             complement(18607..19329)
                     /locus_tag="U4B62_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2140738.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19408..19914)
                     /locus_tag="U4B62_000557"
     CDS             complement(19408..19914)
                     /locus_tag="U4B62_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2140739.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            20037..21836
                     /locus_tag="U4B62_000558"
     CDS             20037..21836
                     /locus_tag="U4B62_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN2140740.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21904..23514)
                     /locus_tag="U4B62_000559"
     CDS             complement(21904..23514)
                     /locus_tag="U4B62_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2140741.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTG
                     SSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCS
                     GFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGG
                     QYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(23769..24278)
                     /gene="mreD"
                     /locus_tag="U4B62_000560"
     CDS             complement(23769..24278)
                     /gene="mreD"
                     /locus_tag="U4B62_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN2140742.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24282..25136)
                     /gene="mreC"
                     /locus_tag="U4B62_000561"
     CDS             complement(24282..25136)
                     /gene="mreC"
                     /locus_tag="U4B62_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN2140743.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25291..25929)
                     /locus_tag="U4B62_000562"
     CDS             complement(25291..25929)
                     /locus_tag="U4B62_000562"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN2140744.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25930..26580)
                     /locus_tag="U4B62_000563"
     CDS             complement(25930..26580)
                     /locus_tag="U4B62_000563"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN2140745.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26593..27492)
                     /gene="rsgA"
                     /locus_tag="U4B62_000564"
     CDS             complement(26593..27492)
                     /gene="rsgA"
                     /locus_tag="U4B62_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN2140746.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27657..29726)
                     /gene="pknB"
                     /locus_tag="U4B62_000565"
     CDS             complement(27657..29726)
                     /gene="pknB"
                     /locus_tag="U4B62_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN2140747.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29723..30463)
                     /locus_tag="U4B62_000566"
     CDS             complement(29723..30463)
                     /locus_tag="U4B62_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN2140748.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30476..31834)
                     /gene="rsmB"
                     /locus_tag="U4B62_000567"
     CDS             complement(30476..31834)
                     /gene="rsmB"
                     /locus_tag="U4B62_000567"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN2140749.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31834..32772)
                     /locus_tag="U4B62_000568"
     CDS             complement(31834..32772)
                     /locus_tag="U4B62_000568"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN2140750.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32765..33256)
                     /gene="def"
                     /locus_tag="U4B62_000569"
     CDS             complement(32765..33256)
                     /gene="def"
                     /locus_tag="U4B62_000569"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2140751.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33271..35679)
                     /gene="priA"
                     /locus_tag="U4B62_000570"
     CDS             complement(33271..35679)
                     /gene="priA"
                     /locus_tag="U4B62_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN2140752.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35932..36237)
                     /locus_tag="U4B62_000571"
     CDS             complement(35932..36237)
                     /locus_tag="U4B62_000571"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2140753.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36242..36856)
                     /gene="gmk"
                     /locus_tag="U4B62_000572"
     CDS             complement(36242..36856)
                     /gene="gmk"
                     /locus_tag="U4B62_000572"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN2140754.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37343..37627)
                     /locus_tag="U4B62_000573"
     CDS             complement(37343..37627)
                     /locus_tag="U4B62_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140755.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37645..38535)
                     /locus_tag="U4B62_000574"
     CDS             complement(37645..38535)
                     /locus_tag="U4B62_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN2140756.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38555..38773)
                     /locus_tag="U4B62_000575"
     CDS             complement(38555..38773)
                     /locus_tag="U4B62_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN2140757.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38743..40443)
                     /locus_tag="U4B62_000576"
     CDS             complement(38743..40443)
                     /locus_tag="U4B62_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN2140758.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
BASE COUNT        10979 a         8815 c         7356 g        13474 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcggg agtgtttccc gattcgtttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa gcgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt ttaacgactc
      301 agttatctta tcatgtcgtt agtaacatgt caataacttt tttgttatgt ttttgaataa
      361 atattttctt ctgtcttttg gacaagtaat aatataccaa ctaaatttag cttggtcaac
      421 gaattttttg tattttttca agtttttttc aataaacgat tattcataac ttttctttct
      481 ttatcgttcg ttttcgtctt ctttaatgtc tagaatagtc ggcattagct tttgaacttt
      541 cgttattatt tattcctttc tacacagcaa aagctgccta aggaattcct tagacagctt
      601 ttattcgatt atctcattgt tgggaatagc aaaacatccc gaattgattg tgcatcagtt
      661 agcaacatta ctaatctgtc gatcccgatg cctagaccac cagttggcgg cataccgtat
      721 tctaaggctt ccaagaagtc ttcgtctaca ccgtgagctt cgtcatttcc tagttcacgt
      781 tctttttctt gcgcttcgaa tctttctctt tgatcaattg gatcgttcag ttctgtaaag
      841 gcattagcaa attcgcgccc cactatgaat agttcgaatc gatctgtgaa tcgaggatct
      901 tctggatttt tcttcgctaa aggcgaaact tcgactggat gtccgtatac aaacgtcggt
      961 tgtgtcaatg tttcttcaac aaatgtttca aagaattcat tgataacatg tccgacaccc
     1021 atagcttcgg ttacttctac attgtgtttt tcggctaaag ctaatgcttc ttccaatgtc
     1081 atttcttgcc agaagtctac accagtttgt tctttgattg catctaccat atggatgcgt
     1141 ttaaagtcac tttctaagtc gacagcttgt ccatcatatg tgatttttgc tgttcctagt
     1201 actttttcag ctgcgttacg gataatacct tctgtcagat tcattacatc attgaaatca
     1261 gtatatgctg tgtatgcttc taacatcgta aattctggat tatgtgtagt atcaattcct
     1321 tcattacgga aaacgcggcc gatttcataa actttttcca ttccgccaac aattagtcgt
     1381 tttaaatgaa gttctagcgc aatacgcaaa tataaatcaa tgtccagtgc attatgatgc
     1441 gtgataaatg ggcgtgcggc agctccacct gcctcattgt gcaatactgg tgtttctact
     1501 tcaatatagc cattgccatc taaatagcga cggatttcgc tgataatttg gcttcgtttc
     1561 ataaatcggt caaagctttc gcgattactg attaaatcca agtagcgttg tctgtagcgt
     1621 tgttcaatat tcgttaagcc atggtattta tctggcagtg gacgaagtgc ttttgacaaa
     1681 atcgtgattt ctgaagcttt gatagaaact tcgcctgtgt ctgttttcat gatttgtcca
     1741 gttacaccaa agaaatctcc aaggtctgca tgtttgaata cttcataggc ctcatcacct
     1801 acttggtctt ttcttacata aatttgaatt tggccttcac gatcttgtag atgcgcaaat
     1861 ccagctttcc cttttcctcg gcgagtcatc atacgaccag ctacactagc ggtcaatccc
     1921 atttctgcta gttcttcttt tgttcttgga tcaaatagtt catgcaattc ttctgagttg
     1981 tgcgtgcgtt caaatcgttt tccaaatgga tcgattccat cttcacgcaa ttgttccatt
     2041 ttttgacgac gtacaagcat ttgatcattt aaatcttcta cttgatgttg ttgttcctgt
     2101 gtcacaaaag ttcctccatt ctcatgttaa tctctttcct ataatgccaa tgaaacacaa
     2161 aaaaagcaag ccgaacttgg aatttcggct tgaaaattca cgaaaaactt agaaatcaca
     2221 acgtttgttt caatagtact ttttccgctt tttctacaaa ttgatccagc aggtcattga
     2281 tttcttgctg tttttctgtc tgattgattg ctactttcac tttcgctgct cgaggaatcc
     2341 cttttaaata ataagccgca tgtgttctga attctctaca agctactttt tctcctttga
     2401 gatctgctaa gcgttgtaaa tgaagtttag ctgtagcaat tttttcggct ggtgttggtt
     2461 caggaatcag ttcccctgtt tcgagataat gctgtgtgcg gtgaatcatc catggattgc
     2521 ccagcgctgc acgaccaatc atcacaccgt ctgcaccgac atagtcaagc atccattttg
     2581 catcttctgg cgttttgaca tccccgttcc ccataaatgg aatcgtcaaa tgttttttca
     2641 cttctttcaa tacttcccag tccgcttttc cttcatacat ctgaacccgt gtacgtccat
     2701 gcatagcgat ggcacttgct ccagctgatt gtgctgctaa tgcattttct acagcaaaga
     2761 cgtgctcttc atcccaacca atacgcatct tgattgttac tgggatattt actgctgaag
     2821 aaacagcttg gaccatttcg tatactttct caggatctaa aagccatttt gctcctgctt
     2881 ctgctttgat tactttattt actggacagc ccatattgat atctattata tcggcagctg
     2941 tgttttcttc gacaaattga gcagcttcaa ctagactatc tttatttccg ccaaatatct
     3001 gtaaacttaa tggatgttct gtttcttcaa tgtggagcat ctctaaagtt tttttgtttc
     3061 tgaaatgaat accttggtca ctgatcattt cacatacgac taagcccgca ccaaattctt
     3121 tgacagtgac tcgaaatgct gaatttgtta ttccagccat cggtgcaaca acgacacgat
     3181 tagggatttc cacattgcca attttccatg tgtgttccac ttaaatccct cctaaggctt
     3241 ctttcttcaa tccatctaat tcttcttcgc tatacttgta tttgttatta cagaaagaac
     3301 aaaccgcttc tgcaccgtga tcttcgtcta tcattgcttg gatttcttct gcacctaacg
     3361 cgataattgc ttctgcaaat ttttcttttg aacagtcaca tttgaattga acaggcattt
     3421 tttccaaaat gttcaattct tctgtaccaa gcaatccttt caatatttct tctggtgtct
     3481 gtccttcatc taagagggta gagattcttg gtgtcgtttt taattgttct tcgatcttgc
     3541 tgattgtttc ttcatcggca cctggcatga tttggatcat gaatcctccc gccgtcttga
     3601 tgctgtcgtc agtattgacc aatacactca gtccaacagc tgaagggatt tgttctgaga
     3661 tagcaagata gtaagtaaaa tcttcagcga tttctccaga aacaatcggc gtttgcccag
     3721 aaaaaggttc ttttaatcct aagtcttttg tgacagtaaa cattccttgt gttccaactg
     3781 ctccccgcac atcgatttta ccttcactat ttagaggaag actgagatgc ggatttttga
     3841 tgtaaccttt cacatctccc tttccatttg catctactac aattcctgtt gctggaccat
     3901 cgccttggat tcgaacagtt agtttgtctt ctcctttgag cgtcgcacca agtagcagac
     3961 taccgaccaa tgtccgtccc aaagcagctg aggaggtgtg ccaagtatca tgacgtcttt
     4021 gtgcctccgc tacggtattt gttgcttgaa cggcgtatgc tcggacgaat ccgtcgtgag
     4081 caagtgcctt aaccaaataa tctttcattt gttatgtact cctttttccg tttttaaact
     4141 catagtgaat catactagca actactgctt ttttcaactt tggaatgctt cttttaaaaa
     4201 taattcagaa tcatctagaa aacaggacat caccctattt acaacttaaa taaagaaatc
     4261 tcccgaaaac cggtttttgt tccggttttc gggagtcata tcagatcaat tgacctatat
     4321 ttttttattt gtattgatcg tctgaatgat tatcttctga ttgttgagaa tctgaatcat
     4381 gttctgcttt attttcctca ttcagttcat gttttgcttc ttcaaaatca cgtttttctt
     4441 ccgcttgttt ttcagcgtct ttttcttcca aagcacgttt tgcttcttca aaagttttcg
     4501 cttctttttc gcttggataa tcatttgact cagtaccttc aggcatcaca ccgtgttcaa
     4561 acaatgactt gattgctttg gcatccaatg tttcgaattc caaaagtttt tcagcaatca
     4621 gtttatgctg tgcacgatga gcttcgatga tttctcgagc tttatcatga gcttccatca
     4681 agatttttcg tacttcttga tcaatctcga aagcgacttg ttccgagtaa gcttttgttt
     4741 gaccatagtc gcggccaaca aacacttgat ggtttccttc atattgaaca ggacctaaac
     4801 gatcactcat tccatattct gttaccatgc ttcgtgctag agctgtcgct tgctcaaagt
     4861 cattggaagc accagttgat tggacattga agatgatttc ttcagctgta cgtccaccaa
     4921 gtaagcctac gatttgttca aacatatctt ctttcgtcat caagaactga tcttcttttg
     4981 gtaaagcgat catatatccg ccggcgcgtc cgcgagggat gatcgttact ttgtggacga
     5041 cccgagcgcg actcaatact aatccgacaa tcgtatgacc agcttcgtga taagcaacca
     5101 tttcgcgttc ttttttgctg attacgcgat cttttttagc tggacctgca atcacgcgat
     5161 cttccgcttc atcgatatca gaagcatcga ttttcttttt atttcttcta gcagctacta
     5221 acgctgcttc attcaagaca ttttctaaat ctgctccggc aaaacctggt gtctgttgag
     5281 ccacaacttt caagtcaacg tcatctgcca tcggtttatt ccgtgcatgg actcgcaaga
     5341 ttgcttcacg accttttaca tctggacgtc caactaaaat ctggcggtca aaacggcctg
     5401 gacgtagtaa cgctgggtct agtacatcag aacggttggt tgcagcaatg acgattactc
     5461 cttcatttcc gtcgaagcca tccatttcta caagcaattg gttcaatgtt tgttcgcgtt
     5521 catcatgtcc gccgcccata ccggcaccac gttgacgacc taccgcatca atttcatcaa
     5581 tgaaaatgat agctggtgcg tttttcttcg ctgtttcaaa caaatcacga acacgacttg
     5641 ctccaacacc gacaaacatt tctacgaaat cagaacccga gatcgagtaa aacggtactc
     5701 cagcttctcc ggcaacagct tttgctaata aggttttacc tgttcctgga ggtccttcta
     5761 aaagtacacc tgccgggata cgggcaccta attcaacaaa tcggcgagga tctttcagaa
     5821 attcaacaac ttcaactagt tcttgttttt cttcttccgc acccgctaca tcagaaaaac
     5881 gtacgcgatt agcttttttg tctgcttctt tggcttttga tttaccaaag ttcatgacac
     5941 ggccgccacc gccaccgccg ccgccttgtt gtcccatcat catgtaaaag aaaaagatga
     6001 tgattacgat tggcaagaag ctaagcaata gggaaaccca tataccgctg cttgattctt
     6061 cttttactgt tgtagcaact ttgttttctt ttgccagttc tgttacttgg ctcaatgttg
     6121 tatcatttgg caaaatgatt gttgtgaaat gggtagtagt cgtatctgtt gaacctaaga
     6181 tagctaaccc accgctgtta gcgacggtct gctgttcttt gtattcacca gtgatcttat
     6241 atacaccatt tgcaggctgc acttcaaatt ctttgatctt gcctgcttgt agttgttctg
     6301 tgaattttga gtactcgata tctgacgatc gctggccgtt attaccaaaa atgaaataaa
     6361 cgaccattac catcgctaag attacgagga cataatacaa ggcatttttc atcatgccgt
     6421 tgttcttttt gttcatagtt gccctcccta agcgtcaatt taatttaaca gttttttatc
     6481 aatcctgacc attctattgt accataattt aattgagata cagcattaat ttgattgata
     6541 aacactaggt ttcaatacac cgatgtacgg aaggttacga tatgcttctg cataatccaa
     6601 gccataaccg accacaaatt catttggtac gttgaagcca acataatctg cttcgatatt
     6661 tactactctt ccttcaggtt tgtcaagtag tgtaacaatt ttgactgatt ttgctttgcg
     6721 gtatttgaaa agatccacta aataagcaag tgttcttccg ctgtcaatga tatcttcaac
     6781 gatcaacaga tcgcgacctt ctacattcgt atctaagtct ttgacgattt ttacttcccc
     6841 tgacgagact gttgcattcc cgtagcttga aacatccatg aaatccaatt ccaaatacgt
     6901 atcgatagaa cgaacgatat ctgccatgaa tggtaccgct ccttttaaga cacctactac
     6961 taggggattg gtgttttggt aaatttctgt taactctttg cctagttctt tgcaacgaac
     7021 ttgtatctct tcttgtgaga tcaaaattct ctcaatatcc ttttccaaca tcaggttctc
     7081 cttccaattg cttctttttg atatttgtaa agaagtatgt agtgtatttt atcagtttct
     7141 tcacgaatac tcaaatatga aaaggcaaaa ggtatcaagc tatatatatt tcttttggca
     7201 tctgtaatga tccaagcatg ctgcctttgt gaattaggta ctttctgatc gataaaatat
     7261 ctagatactt tttttgtgag attctctgtt aatttaatgc ggtctcctgc ttttcttttt
     7321 tcgacataca gttgcacatc agcaggaatc caaatgtcat gagaaaatag agaccatgaa
     7381 tctgttgctt tgatgtcccc tgcattttca ggtttgacca gaccaatcca ttctgtttca
     7441 ttcaaatata ttccttgttc tggaatcagc aacaagcgct ggtcttctgt ctggctagtt
     7501 gcttgttttt cttttttaga gagtacaaca aaatgatact ctcttttcag tatccaatta
     7561 ttgccaatac tgagttgcca ttggcttttt ttctgttgcc actggtctaa aatcgactcc
     7621 atttgttgtt ctttcaaaat aattcctgtt tttgagaatg ctttgtcgaa aaaggagtaa
     7681 aaaaaatagt atcgttcagc atctgtcatt tcttcaatca tttctgcaga aaatcgaaat
     7741 tgttcctccc actgttcgac ttccttttcg atcaactgtc ccatatattt ttgaatgact
     7801 tggtctgccc attggatctg ttgcgaaaat tgctggaaat gtctcaacgc ctgtgtattt
     7861 tcttgtttca attgaggaac taccaaatga cgaagccgat ttctctggat atctaacaat
     7921 tggtttgtct gatcctcaaa gtaaacaagc tgcgattccg ctgcataagt atataggtct
     7981 tctttgctaa aactcagcaa cggacgaatc agtctgcccg tagcaaatgg ttgtacttct
     8041 ttgattccag aaaaagtatt taactgtccc ccacgaacga ttttcattaa caccgtctcg
     8101 atttgatcat ctccgtgatg agcagtcatc aacgtgtcat attgtccttc ttccatcacg
     8161 cgagtgaatt cttgataacg aaagttacga gcttctgttt ctatggcgga tttggctgga
     8221 tcttcccaaa tacttacata taaagggaga ttcttctctt cacaatatga gcgaagatag
     8281 gcagcttctt ctgctgattc ttctcttaat tggtgattga tgtggacgat acctaaagaa
     8341 aaaccgattt ggcgttgcac ttgtaaaagt aaatggagca aaaccatcga atcaacacca
     8401 cctgataccg ctgctaaaat tctcgatcct tgttgccaga atccttgttc attcccgtgc
     8461 cgaataaatg actgctgtaa tagcggtgct tttttcccgc ctttttcaac gttcatcctt
     8521 tccacaccct ttcttcctaa aaaaaagaga gcgggaaaac gtctaaaacg acaattaacc
     8581 tgccctctgt tgatactatg acccaaatag tcgcactatc attatttgcg gctttttagc
     8641 tgcgacgtcc gccacggcca ccgcgctttc cttctgtgtt tcgcttcaat gaagataagc
     8701 ggtcatcgct atctttcaaa aatgaactca tcaatgaatc gaagtcttct ttttgagctg
     8761 aaggttgact gcgagaaaaa gattttttct gtgctggacg atttcggttg tcacggggat
     8821 ctttttgttg atatttttta ggacggcttt cttctccaga ttcagcaggt tgttcctgcg
     8881 ctttccggat agatagtccg atttttccat catcatttac tgaaataact ttaactgtta
     8941 cttcatctcc tacacttaaa acatcatgga tgtcttttac atagccattt gacacttcgc
     9001 taatatgaac tagacccgtt tttccagcac ctagatcaat aaacgcacca aaatttgtga
     9061 ttcctgatac ttttccttgc agttttgctc ctacctcgat tgacataaaa aaaatgttcc
     9121 tcctaattat ttcccttttt tatattcctg cttattttgc agttgtagaa ctagttgatt
     9181 gatctgtttc atttgtcgag ctgtctgtat ttgattctaa tataggataa actttttcac
     9241 cgtcttttga gtaaaagaat ttgcttctgg caagtttagc tacatactca tcatctttca
     9301 gcaatgaaac atcttggtgc agctgatcaa cgtctgcgtt gacttctttc atttcagtat
     9361 ttgcttcgac tctcaattcg ttcaattgat gcatcctttg catgtcatgg aaaatctgca
     9421 cacctattat cgcgaaaatg accaaagcac caacaaaaat tgcagccagt ctgcgtcttc
     9481 gaaaaatcag ttgacgttgt tgttgctgaa attcagcata ctttttcttt gcatattccg
     9541 tatctagcgc agcgatttta ttgctttttt tcgtcacttg gattcgctcc aatctttttg
     9601 attgtctttt tcattatacc aatatcgtga aagcttgtct aatctatttc tctagtgttt
     9661 ctgtgcgatt ttcagaaata atttcgtaca ttttttgtgc atcttctttt ttggttgatt
     9721 catgtaattc cgtaattttc acttccaacg ttttgttgcc aaactggact ttcaaaatat
     9781 cgcctacttt gacatcactg gaagatttcg ccaatttacc gttgatttga attcttcctt
     9841 tatcagctac ttcttttgct actgatcgtc ttttgatgat tcgggaaatt tttaaaaatt
     9901 tatctaatcg catatcttat tcctcacttt ttaatttttt tatttaatcg caggattttc
     9961 ttaccaaaag gcaacattag ccattctcgt actgtaaaca gacgaagacg aatcactgca
    10021 taaatgaaca cagaagcccc gatcccaact cctatcacgc tgacaaaaaa agcagtggaa
    10081 cgatgagtta caggaccaaa tactatattc aatacaccat aatacagcag cagtactact
    10141 gtcatacaaa gaaggctgaa catcaacttc cgtccaaaat gccgttcttt ccaaaaagca
    10201 tttagctctt ttgtttcaat tcgaacaaaa taccaaagcg tcacacctaa tcctaaaagg
    10261 gtagacaggc ttgcacctgc tgttcctaat aaaccggtca gaaaagaagt agtcaatcct
    10321 tttaccaata gtccccatcc tgcgcctttt aaagaagggc gaaacgagtt cttgctttga
    10381 gcaatacttt gataagcttg gatgactgca gtgaaagcaa tggaaaaaac aaataataca
    10441 agcgctgcat ttcccgcata atctttaaac aacgcataat tgatataagg gagcagcaag
    10501 gctaatccca aagacgcagc taatgctaaa gcggtcgtca atctaagata gattttggct
    10561 gtctgtaaaa actgacgata gatccggttg gtcaaatgac gagtcaacgc aggcaggaat
    10621 gtcgcactta atgcagtggc aatcactaaa cctaattgta ctaaaggctg acctcgatca
    10681 tagacccctt ttgctatctt cgcaccgtat tctgacagtc cgtaaacttc caaagcattt
    10741 tttacaaaga aagagtcgat cagctggaaa aagataagca atccactata aatcgaaacc
    10801 agtccgcctt ctaccagaaa acgccggatc aagggctttg acggctgttt gaacaatgga
    10861 aaccgtcgga agcttaacgc tcctccatga atcttttgtt catagtagtg aaggatccca
    10921 tatgcgcata aaccgccaaa aacagctcca ctcattgctg ccgtccctgt ttggtacacc
    10981 gtccatccaa gctttctaaa agcaaacgca ctacagacaa tcacaaaaac acgaacgaac
    11041 tgctcaacta cttgagaaac agcagttggt tccatcaaga atcttccttg gaaattccct
    11101 cgataaaaag atagaccagg catcagcaaa aaggtaaagg aaactacttg gatcagcggt
    11161 ttcaattgta tgtctcccat catccaagca atccagctgc taaatgcaaa gacaaacaac
    11221 cacaaaaaga cacctgtcca aaatactaag ggataaagat tttgcaaagc ttgctttcgc
    11281 tcgaaaggat tctttctttc tgcaacgtac tttgatatga actgcggaag accggataaa
    11341 gctaaagtca ttgctatccc ataaattgga taaacttgct gataaacata aaaaccttcg
    11401 tcgccgacta agttttggag gggaattcgg taaagggcac ttaatacttt tgctatgaaa
    11461 gaagcaatcg tcaatacaaa tgctccttgc atgacgcgcc gcatttcttt atgatccaaa
    11521 tcattgctcc ttcctacgct gacagatatt tttgctcacg caacgctttg acaaactttt
    11581 ggatttcctg aagccatact gcttcttcca tttggttggg gatcgtcaag cgtatcacca
    11641 tctgttcttt ttcaacgcct agactggctt ttagttttgt cgctgtcagt gcttcgaata
    11701 actgttccac actatagcgt tttgttccaa ctttacttag agtaaagact atatgcgcct
    11761 cctgtttcct tatcgtttct aggagcgctc gatcgccatc cattttgatc tgcccaattg
    11821 tcaagagatg tgctacttct tctggatatt ctccaaaacg gtcgagcaga tcatcttcta
    11881 gttcatctag catttcttgc gaatcaagtt cacgaattcg tttatagatc tcgattttct
    11941 gccgttgatc tgccacgtat gtttcaggta gatacgcatc gacaccgagg tcgatctcaa
    12001 cagtggtctt ctccacttga ctattctttc cttgtttacg agaaacagct tcgcttagca
    12061 tttgagaata catatcgaaa ccgactgcat caataaatcc atgctgctga gcacctaaga
    12121 gattacctgc accgcgaatg gaaagatccc gcatcgcaat cttgaatcca gatcccagtt
    12181 ctgtaaaatc tttgattgcc tgaagacgtt tttcactgac ttcgttaagg atcttctgct
    12241 gctcatacat aaagtaagca tatgcgacac ggctgcttct tcctacccgc cctcgcaatt
    12301 ggtataacgt ggataatccc atataatcag cgttctcaac gaacaacgta tttgcgttcg
    12361 ggatatctac tcctgtttca atgatggttg tagtgacaag aacatcatat tgaccttcaa
    12421 taaattcaaa aagcgtattt tctaattgcg cctctgtcat ttgcccatga gcataaccga
    12481 tccgtgcttc cggaacgagt tcttggattt cttctacttt ttgttcgatc gtttctaccc
    12541 gattatataa atagaatact tgtccacctc gtcccatttc tcgatggatt gcttcgcgaa
    12601 tagccccagg atttttttcc ataacatacg tttggatcgg ataacgattc gcaggcggtg
    12661 tttcgattac tgaaagatcc cgcacgccta acatcgacat atgcaaagtt ctcggaatcg
    12721 gtgttgcagt cagagtcaaa acatccactt gtgcacgtaa ctgtttcaat cgttccttat
    12781 gttttacacc aaaacgttgt tcttcgtcaa tgatcagtag acccaaatcg ctaaattcaa
    12841 tatcttttga caaaattcgg tgggttccta caacaatatc aacttgacct gtacgcagtt
    12901 gttcgatggt ttctttttgt tgctttcttg ttctgaaacg acttaatagc ccgatattta
    12961 cggggaaacc ttcaaatctt tccagcatcg tttcatagtg ctgctgtgcc aagatcgttg
    13021 ttggaactaa aaaggctact tgtttgcttt cctttatcgc tttaaaggcc gctcgaagcg
    13081 ctacttctgt tttcccataa ccaacatcac ctacgagtag tcgatccatt ggtttttctt
    13141 tttccatatc ccgtttgatc tccgctgcac ttctcaactg gtcatcagtt tcagagtagg
    13201 ggaaggcatt ttcgaattct ttttggtagg cgtcatcagg tccaaaagca tatccttttt
    13261 ctgactctct tgcggcatat aatttgatta gatcgtcagc gatatcctcg atttttgaag
    13321 agacttttcg cttggttttc gtccattcac ttccgcctag cttattgatg cgaggcgctt
    13381 tggcttcaga ggcgacatat ttttggatca gattcagttg agtaacagga ataaacagtt
    13441 tgtcgtcgtt ttgatacaaa atagtcatat agtcttggtg aacgccatct acttctagcg
    13501 tttccatccc aatgtatttc ccgataccat gattggcatg gacaacgtaa tctcctgctt
    13561 ttaactcatt atagcttttc aatcgctctg cgtttgttac tgtttgacgt ctcgtttgtt
    13621 ttttcgtcgt tttttggaaa atttcttttt ctgtgatagc tactattttt tcttgaggaa
    13681 gttcaaaacc agcctgcaaa gctccttcaa ctaattgtac ctgtcctctg atcaatgcat
    13741 ttttttctgt ttctactact aatatgtctt catctcgaag catttgttct gctttgcgta
    13801 ttctgtcctt tgtcggaata aagatcacaa cagtctgttt ttgcttgcgc caacggtcca
    13861 tttctgtttt cagcaatggc atttgtccga agaactgttg catcgtccga tattgaaaat
    13921 tatggattgc ttggaaacga agatttccca tccctttttg gaagagtgag aaaaaggttg
    13981 tggtgaactt ttccttctgc acgatttttt gtatatctgc tgaaaatgtc tgctcagaaa
    14041 acacccgcaa ttcgctgatt ttttgagtct gccattctgc cgcttccctt tcgatttccc
    14101 gatttgtttc catgattcgc tgataatcat caacgactag taaactattt gaagggaaat
    14161 aatccaagat cgtcacagat tcttggtaaa gaagatctga gtagaatttt gcttcttctg
    14221 tcgggacacc tgcttgccag cttgaagtca gctgaccgaa gtaatcttgc aaaaactcgc
    14281 ggtctgcttc ttctgtggtc gcagcaagcc gtttttctag cattttttgc atttgctcta
    14341 ttccatgttc tagatcctct ggtccgtaga cttgttcact cgttggggaa acccagattt
    14401 cttccgtttt ttcgattgag cgttgtgttt ctgcttcaaa ataccgcata gaatctatct
    14461 ctacatcaaa tagttctaca cgaacaggat aggttgtcgt taacgggtaa atatcgataa
    14521 tgcttcctcg aatactgaat tctcccggtt ttgcgaccat ctcttctcgt acatagccca
    14581 tcaaagtcaa ttggcgagga agattttcca cgtctatctc atcaccaata tgccaattca
    14641 attgcgcctg tagccaagtt ttcttattcg gcagtcgttt gtgcaaagat gcgactggca
    14701 acaaatagat acctgctttt ttttcttgaa gtgcgttaag cgttgcaact ctctcgcctc
    14761 gcgcttctgg agaagaaaag gccatttcag cagaaagcac ttcatccact ggaaataaat
    14821 ggatctgatc ttgcggaatc acatgttgca gatcttctgc taactgattc gcataataca
    14881 aatttggcat cagaataatc atgtttttat ttgttttctt aaacgctcga gcaaatagaa
    14941 gtgttttcgc tgagcccgct aatcctgtga ttagttgtct aggcattttt tcttctaaat
    15001 tgctgaccca ttcttttact tgcggatctt ttccgactaa ttgaataata tccattcctt
    15061 ttcctccttc ctcaattgaa tcgattcatc gcatccacga acgaatgccc ttctatgaga
    15121 tattcgactg ctttaacaga ttgacacatg ctttcttcta tcaaaggctg attttctttg
    15181 ctgaaaggac taagcacatg ctggactact gtttttttcc cttctggcct gccgattccc
    15241 accttgattc gatcaaagac attcgtattc aaatgggaaa taatactttt gataccgtta
    15301 tgcccgccgg cacttccctt ttgacggagc cggatttttc ccactgccag atccaaatca
    15361 tcgtagatta cgactaattc ctctggataa atcccaaaat aagtcattag tgggccgact
    15421 gcacgaccag attcattcat gaatgtttgt ggtttgacta aaaggatctt ttctccatta
    15481 tgaaaaaatt cggcaacttc tgcttcgaac ggattttttt tgaaagtagc ttgatgctcg
    15541 cgtgcaattt tatccacgac cataaagccg atattgtgtt ttgtatattg gtatttggta
    15601 cctgggtttc ctaacccgac gatcattttc atttttgttt tccacctaat tctaaaaagc
    15661 agcacaaaag gctggactgc taattagcgg tccagtccat cgagcttttt tcttttttac
    15721 acttgtcaat gcatagtata acacaaaaca aggcgtttcg gctaaaagag aatcgttaat
    15781 aaatgataaa gaaattaaaa atttcttcaa agtttaatga aataattgat atataaaaat
    15841 agtgaaataa cgcacatgta agcttttaca tataatacaa ccttgttatc tgttatattt
    15901 tatagccaaa tagcgtgaca tggggcttga gaaatgttag cataagcatg ggaaaaaaca
    15961 aatcaatgga aggattgata ctaaatgact gcagctgcag aaaaaaaaga tcatcaaaaa
    16021 gttattcttg ttggagacgg cgccgtaggt tctagctatg cctttgctct agttactcaa
    16081 aatatcgctc aagaagtagg tattgtggat atcaatactg ctaaaactga aggagacgca
    16141 attgacttgt ctcacgcttt ggcatttact tcacctaaaa aaatctattc tgctacttac
    16201 gcagacgcac atgatgctga cttagttgtt attacagctg gcgcaccaca aaaaccaggt
    16261 gaaactcgtt tagacttagt aaacaaaaac ctaaaaatca accgcgatgt tgtcacacaa
    16321 atcgttgaat caggatttaa cggtatcttt ttagttgccg ctaacccagt agatattttg
    16381 acttactcaa cttggaaatt ctcaggtttc cctaaagaac gcgtaatcgg ttcaggtacc
    16441 tctcttgact cagctcgttt ccgtcaagca cttgcggaat tagttgacgt tgatgcacgt
    16501 aacgtccatg cttacatctt aggtgaacac ggagattcag aattcccagt ttggtcacat
    16561 gcaaatgttg ccggcttgca aatctatgaa tgggtaaaaa acaacccaga aatcgatgaa
    16621 gaagctatgg ttaacctatt ctttggcgta cgtgacgctg cttacacaat tatcgagaaa
    16681 aaaggcgcta ctttctatgg tatcgcagta gcattagcac gtatcactcg tgccatctta
    16741 gatgatgaaa atgctgttct tcctttatca gtattcatga atggcgaata tggattgaac
    16801 gacatctaca ttggtgcacc tgctgttatc aaccgtcaag gtatccaaaa agtaatcgaa
    16861 attcctttaa gtgacagcga gcaagaccgt atggctgctt ctgctaagca attaaaagaa
    16921 attctagatg aagcttttgc taaactagac gctgaataat ctttgaacat gacggaaaac
    16981 atctattcgg tgttttccgt ttttttattt tatcaccctt atggtttcta aattttttca
    17041 taaagtagag ataaccttcg tcttttcctc taaattatgc tatcatgaga ccgttccacc
    17101 ctaaactttt acttaaggag ttgtcgatat gacaagatct tcgcatcgaa aaaaatcgaa
    17161 acatgttgct ttgctttcag ttcttggtgt tctcttagtg ctgatcagct tttataccta
    17221 tcgcagtaca tactacacaa agcatttttt gccgaataca gaaataaatg gaataaatgt
    17281 cagcaatcta acagtcgaaa aagcaaatga aaaattaaaa gaagcttatt ctgataaaaa
    17341 actggctatt aaagaagatg gaaaagtctg gcaggaagta ccaaaatccg agctcggcta
    17401 caaagatgat ttcacttcgg agctttctcc gatcctaaat gaacaaaacg gatggacttg
    17461 gggaatgact tatgtctcag cggcagaaaa acaagaaata gatcctgtca gtcaaaacca
    17521 ccaaaaacta gacactaccg ttcagacatt gacaactaag ctgactgatt tgaacaaaga
    17581 ccggacacct actactgatg caacgatcga aaaagcaggt gactctttcg ttatcaagcc
    17641 tgaagtcaat ggaaatacga tcgacgtcga tgctgcggtc aaacaattaa aaagtgcggt
    17701 aaactctggt aaagatacaa ttgaattgac tgaattcaaa gaaaaaccaa aagtaacatc
    17761 tgaagacagc tcactgaaag aacaactcgc atccatgaat gctatcgcaa atgtaaaagc
    17821 tacttacagt ataaacggtg agactttcca gatcccatca agtgatatta tgagttggct
    17881 gacctacaat gatggaaaag tcgatttaga tacagaacaa gtacgtcaat atgtaacaga
    17941 tttaggcaca aagtataaca caagtacgaa tgacacaaaa tttaaaagta caaaaagagg
    18001 agaagttacg gtcccagttg gcacttacag ttggacgatc caaacagaca gtgagacaga
    18061 agcattgaaa aaagcgattc tggcaggcca agattttact cgttcaccca ttgtgcaagg
    18121 tggcacaacc gctgatcatc cattgatcga agatacgtat atcgaagttg atctggagaa
    18181 ccagcatatg tggtattaca aagacggaaa agtggccctt gaaacagata tcgtttccgg
    18241 aaagccaact acacctacac ctgctggagt cttctatgtt tggaataaag aagaagatgc
    18301 aactttaaaa ggaacaaatg acgatggaac accttacgag agtccggtaa actactggat
    18361 gccgattgac tggacaggtg ttggtatcca cgattcagac tggcaacctg aatacggtgg
    18421 agacttatgg aaaacccgcg gttcacacgg ctgcatcaac accccaccaa gtgtaatgaa
    18481 ggaattattc gggatggtcg aaaaaggaac gcctgtattg gtcttctaac aaaaataata
    18541 tataaaaaga aagcacccgt acgattggta caggtgcttt ctttttatta tctaacaatg
    18601 tgctacttac atttcagcag cagtaaagac ttctgaaaca tcatcatcgt cttctagctt
    18661 atctactaga cgttccaatt gctctttttg ttcctcattt aaatcaatcg tcgtttgtgg
    18721 aatcatcgtc aattctgctt gcgccaaagt gaactctttt tccaactcat ctcgtacagc
    18781 agcaaaatct tctggtgcag tataaatctc gaatacttca ggagaagttt gcagatcttc
    18841 tgcccccgcc tctaatacat cctcaaacat cgtctcttca tcaacagtca acccatcgcg
    18901 ttcaatagcg atatatcctt tgcggtcaaa caagtaactg actgaacccg tttctcccag
    18961 tgatccaccg tttcgagtaa aagctactcg gacatttgtc gctgtccgat tacggttatc
    19021 tgtcaatgca tggactaaga ttcctactcc acccggacca tatccttcat acgtgatttc
    19081 atcgtaattc gtttcatcta cggcactcgt agccttttta atcgcccgtt ccacattgtc
    19141 gttaggcata tttgcagctt ttgctttgtc cacaacaagc cgcaaagctg gattcataga
    19201 tggatcagga ccgcctgctt tagctgccat ataaatttca cgagaaagtt tctgaaagac
    19261 tttccctcgt tttgcatctt gggcattttt tcttccttgg atattatgcc atttactatg
    19321 tcctgacatg atttttcctc ccttgttctt ttgagaatac acttctattc tagtactatt
    19381 tcacaaaaaa acaaagccac agccaatcta taaacgcttc ttgaatgccg caaaaagcac
    19441 acataatatc acagcagtca gagcaccgat cgaatccaac atgacatctt ggaacatagg
    19501 tgttcgtcca ccggtcatca tctgatggaa ttcgtccaaa cctgcgtacc ctgttgctga
    19561 aagccaagca aggatcgctg tcagccacca aatttttagc ttggggaaaa cacctaaaaa
    19621 caagctgcct cccaaaataa aataggtacc gaaatgtgcg cccttcctta aaaagaactc
    19681 taaaaatttg gcgtagccca gatggttgat actcaccata cttccaccat aattaaaacg
    19741 tatacccgct aatgtgtctt taaatggctg attaggcagc cagctttcta atcgcgaaac
    19801 ttgtgattgc tgtccgtaag tttgagaaga actgtaaaat aagattataa cgactatcag
    19861 tgcgattact aaataaatat ttccgttttt catttgtttt tttgaagaat tcataagtca
    19921 cctctgtatt ttatcttaga ctttttttga agaaacaaca atcattttca tagagacttc
    19981 ttccataaaa aagtgctata cttaattttg tattacaata tgaagggagt tttttcatga
    20041 gttattcatt aaattggaat ctagattcta ttttttccgg aggcagtcat tctgatgctt
    20101 tgaaccaacg gatgaagcaa ttggaagacc aaatgaacga gtattatcac cgcgtaacaa
    20161 aatggtcacc tagttctgac aatgcagagc agttaaacgc tattcttcaa ttgcaagaaa
    20221 cgattacgaa tggttttacc caatgcagca gctatattac tgcattgctt tctgccaatg
    20281 tcaatgattc agatgcaaag gtcttatcag gcaagcttta cgcaatgctg cctcgtctgc
    20341 aatcagccga aactgtattg tcaaagaaat ttgcagaaat atctgacaat gattggaatc
    20401 agatgctttc tcaatcttcg tttgaaacaa ttgcttttcg tttaaacgag attcgtcgtg
    20461 atggctcaca attattatca gaagctgaag aaaacattat aaatacgctt tctttagatg
    20521 gtctaaatgc ttggagcagt cattatgata cgattgtagc cagcatttcg attcccttcg
    20581 aacaagatgg acaagtagtc gaattatctg ctggacaagc cttcaataag atgatgggcg
    20641 atccagatcc aaaagtcaga gaaacacttt tcgcagcatg ggaagaagcc tggaaagaaa
    20701 aggcacccct cttcacggat actttgaatc atttagatgg ttttagactt tctgactatg
    20761 aacttcatgg tgtcacagat tttctacaaa aaccattaga gtacaatcgg ttgaagaaag
    20821 aaacattgtc tgtcatgtgg gatacaatcc agaaaaacaa acaacccctt gttaattact
    20881 tgactagaaa agctaatctt ttcggtaaag aaaaaatgga atggcaagat caagatgcac
    20941 ctattatttt aggcgatttg aaagaaaaaa cctttagttt tgacgaagca gcagcattta
    21001 ttatcgaaaa ctttaacaaa ttcagtccaa aaatggctaa atttgcccaa tctgcttttg
    21061 aaaagagctg gatcgaagca gaagatcgtc ctgggaaacg tcctggtggt tattgtactg
    21121 agttaccaga aacaaaagaa tcaaggatct ttatgaccta cagtaattct gttaatgagg
    21181 ttgcgacact tgctcatgaa ctaggacatg ctttccatag cagcacgatg tgggatttac
    21241 cttctttaaa tcgcgaatat gccatgaatg tcgcagaaac agcaagtaca ttcgcagaac
    21301 tgatcgtagc cgatgctaca ttaaaagtcg caaaaacaaa agaagaaaaa atcaatttgc
    21361 tagatacaaa actgcaaaat gctttagcta tgttcatgaa tatccattcc cgattcattt
    21421 ttgaaaatcg attctatgaa gcacggcaag aaggattagt ctcagaagat aagatcacag
    21481 agatgatggt tgaggcgcaa aaagaaggct atcaaggagc tttagctact taccatccat
    21541 atttctgggc tgcaaaattg catttcttta ttgatgacgt accgttttat aacttcccat
    21601 acacatttgg ctacctcttt agtttgggca tctatgctta cgccaaccaa aagggagcaa
    21661 attttgaaga agaatatatc catttactac gcgatactgc ctcaatgacg acggaagatc
    21721 ttgcgaagaa acatttgaat gtcgatctta cccaaccaga tttttggcaa gcaggtatcg
    21781 acatggtgac tgaagatatc caaacgttca tggaattgac agaagaattt atctgatttc
    21841 tattttatca caataaaaaa agagttagtc cagaatgggc taactctttt tgattaatca
    21901 ttcttacatg ctgacagcaa agtcaggggc aaaccattga actgaaccaa cttttacgct
    21961 ttcgcctggt tgaggagcat ggatatattg tcctccgcct aaagcaatcg ctacgtggta
    22021 agttccgcct tgtgaccccc agaataacaa gtctccagct tttgcttgag aaactgaaat
    22081 ttttgttcca gctgattctt gaggaactgt ccaaccaccg atgtcacgac cagttacttg
    22141 catgtaaaca tagcgtgtga accctgagca gtcaaagcca cttggatctt taccgcccca
    22201 aacatatgga gtaccaatat atttgtaagc ttctgctacg attgctgcac cattcacact
    22261 tccagaagga gctggagcag gtgttacagg ttttggttgt tcaggtgttg gtgttggcgt
    22321 tggagccgga gtactacttc ctgtgccgtt tcctgtatca acactttgat ctgttgatgg
    22381 tgttgtaggc gcaggtgttg ttgattcttc tgttgttgaa gattcaggca ctgttgttga
    22441 ttcttctgtt gctgaagatt caggcactgt tgttgattct tctgttgctg aagattcagg
    22501 cactgttgtt gattcttctg ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc
    22561 tgaagattca ggcgctgttg attcttctgt tgaagattca ggtgttgttg attcttctgt
    22621 tgctgaagat tgtgtagctg atgatgattc ttcagtcatt gaagattgtg tagctgatga
    22681 ttcttctgtt gctgagcttt gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc
    22741 tgcttgttcg cgtgcttctt tttcagcttt ttcttgtgca gcttgttgac gagcttgttc
    22801 tgctagacgt tgttgttcac gaatacgagc ttgctctgct tcagcttctg ctttttgacg
    22861 gttcaagtca gctttcttgt cttcagcagt tgcttgttca gcagctaatg aagttttcag
    22921 tacgttcaaa tcagcttgtt tagataaaag atcgcctttt tgagattcca aagcagcttg
    22981 gttttcagct aattctttta gtttagcatc gttttcagct tttttgtctt ctacagcttt
    23041 tttatcttgt ttttgttgtt ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc
    23101 ttgaacgcgt ccaatagcat ctgctaatga atcagcattc aatacagcat cgatgtagtt
    23161 tgagcttgtg ttgcttactt gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt
    23221 ttcaatacgt tcttgtaaat ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa
    23281 tgtatcttgt tttgctaata ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc
    23341 taaggcatcg atttgtgatt gtgcatctgc ttgttgattt ttcaagtctg caatcttttg
    23401 atcttgctgt tgtatctgag aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac
    23461 ggcagtcaag gtcattgaac ataccattac tgctgatatt aaactctttt tcactcttca
    23521 ttcctccgac tggcttaatt taataaatat ctaaatatat ttaagtcttt cttaacgata
    23581 taaaaattgt accatagcag tgtgtcaacg atataatagt tatgttacaa aaatgtttca
    23641 aaacaacttt taaaaatgac aaaactgttc gtttcagaaa atgatcatga aaaagccgcg
    23701 ttaagtctag catttcttaa gataacaacg attctttttc gctttcgacc taaaaataaa
    23761 aaaagaaatt attcatcact gaataatttc ttaaagggaa aaatgaataa tacaaatata
    23821 atcatgttca acagcagtgt aggtcctaaa aaccttgtaa taaaatacgt gttatttact
    23881 actgctaatt taaaaatcaa ctggaccacc atcgtaaaca attcataacc cgtgacaaag
    23941 atgatcatac tgaaaaattc agtaaagatg ttgacatgga taacgtcttt cattacatac
    24001 atcaaccata caattaaagg gagagctact gcatagatac cgataacgcc aatatagtaa
    24061 gcgtcataga tagccccgag aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc
    24121 atactgcaac ataataatgc caatatcaga aagtgggcat ttgacatata ggtgcctttc
    24181 gaccactccc ctagcattct tgttaaatga ccatctatca acatcaaaaa gaacaaaacg
    24241 attggcagat agtacttcat tgtttctttc ttcaacatgg actaatcctc cgctagccgt
    24301 tggacaatcg tcacaactga cacgtcatac atttctgcat aaggtttcac ataaacttcc
    24361 cgatccagcc cataactatc tggtttcgtt ttaataaccg taccgatcgg caagttagct
    24421 ggagaattcc ctccaagacc ggatgtctgg acaacatccc cttctttgat atccgtatca
    24481 ccagttaatt gggtcaccac taaggcatgg agcttttcat catagttttt aagcaaacca
    24541 aacgcttcgc cattagccga agatacccgt actggaaaat gattggagct ttcattagag
    24601 gatgacagta attcgatttt agacgaagcc gtattgacct cgatcactcg gccaatcaat
    24661 cctttttgcg acatgactgc catatttgct tcgataccgt ctttagaacc cttgtctacg
    24721 atcagcatgt cctgccaact gtcaggcgaa cgagtaatga catttgccgt tactttttcg
    24781 taactagtca gcgtctgatt caactcaagt tcttttttca acgcttcgat ttctctctcg
    24841 taattcttgt tttgctgtga aacgtcatca tagctatcga ttttttcttt tagccgctca
    24901 ttttcggcat aagttgaaaa aagattatgg acagaatcta ctccatttcg tacccaccgt
    24961 actggggcag agattgtttt atcaaccatt gcaacactgt cattaacgat ggattggaag
    25021 aaattgggct ttccttcatt cgctcgctgc gcagcggtca cacttagtat cgtcaccacg
    25081 atgataacaa taaccaaggt aataataata tttttattag gattgaactt cttcacaaag
    25141 acacctcgaa ttaattagac tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa
    25201 ggtaagttgc tcctcttaag ctttttttta gcaaataata gattctgtca tttttagcct
    25261 gaaagacaaa tttttttatt tgtttatgga tcaatctttg ctttgaataa ctgcaatcat
    25321 accatctgaa aaagaaaatt ctgcggtatc tgaaagaaat tcgttacttg cataagatgt
    25381 cggatattct acttctttat tttccaatac atacttgctg ccttttaatg taagattcga
    25441 gacgggagtc aagcagcaat aagctaaata cttcatatct ggtatctgtc ttactatatg
    25501 ctctccaggc aaataataag acaaataatt ttgacaatct ctgataataa gctgatgggc
    25561 aaagggtttg aagcgaggtt ccatacctaa ccagagattg gctaaaagat gatccaatct
    25621 tccgcccgta gctccaatca aagtgacttc cgcttgcggg taacgttcaa atgtttttaa
    25681 gatacctaac tgtgtatctg tatcgtcttt ttctgcaggg gaacgggtat actctttggc
    25741 ttttgcaaat acttccttct gctcttcgtt cgacaatgaa tcaaaatcac ccactgccag
    25801 atctactggg tagcctgctt gaagcaggta cagcgcacca cggtctatcc cgacataggc
    25861 atcgtatttg gacagcagag actgcggcca gttttgcgga ttacctccag cagctaataa
    25921 aattttcatt tagtctaatg cctcttttaa tcgttcgatt tgtccgactg gattttcggc
    25981 tccatagata tatgatcctg caacgaagac atccgcccct gcatctttac agatttttgc
    26041 tgtttctgga acgattccgc catctacttc gattgcataa gtccaattgt tctctctacg
    26101 tagatcagcg agttccgtaa ttttttctac tgtttcttca ataaagcttt gtccgccgaa
    26161 cccaggattg acagtcatga ctagtacttg atcagccaaa ggcaaaacat gtttgatcat
    26221 ggagactggt gttcctggat tgataactac tccagcttta actcctaagc tttttgtcat
    26281 ttgaagtgcc cgatgaatat gaggagttgc ttcgacgtgg acagtaatga tatctgctcc
    26341 agcttttgca aatgctggaa tgtagttttc tggatcaacg atcatcagat ggacgtctaa
    26401 cggtaattta gtaacaggac ggattgcctg aacaatattt ggccctaatg tgatatttgg
    26461 gacaaatcgg ccatccatta catctacgtg gatgtaatct gcgcctcctt tttccactaa
    26521 ctcaatgtct ctttgtaaat tggcaaaatc tgcactcaaa atggatggtg ctatcttcat
    26581 tcttgtttcc tcctattttc gtttaccttc tttttttgca tacattggtt ttcttttttc
    26641 gatttcttgt aagaattgca aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc
    26701 tactctatgt tttacttcgc agccaggttc tttagcatgc atgcactctc taaatttgca
    26761 atggacagaa gcttctacaa attcaggaaa ttgtctcggc agttctcctg cttcaattgt
    26821 caaaaagtca atcgagctaa atcctggtgt atctgctact aacccgccat agataggaat
    26881 cagttccaca tgacgtgttg tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt
    26941 tgctaaattc agttccggtg atatttgatt cagtagcgtc gattttcctg ctccagactg
    27001 acccataaag accgatagcc gttctgggaa gtaacggatc agttctgtcg tatcacccgt
    27061 atgattgggt acaatcaccg gatagcctat tttagtatag gtttgcttga tctcttcgat
    27121 tttttctggg tcgtctgaca agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc
    27181 gtattccaag gtaactagaa agcggtcaag tagattataa gaaaatgcag gctctacgat
    27241 acttgtgaca atgattccaa gatcaacatt tgtgactggt gggcgaacta attgattttt
    27301 tctcggcaag atttccagaa gatagccgtc tgtttgattt tcactctcaa agatcacctg
    27361 atctccgaca agcggcgtga ttttccgatt ccggaaattt cctcttcctc ttgtttgata
    27421 ggtctctccg tttgcgtata catagtaaaa accgctgatt gcttttctga tttgtccttt
    27481 gataagtgcc acctccgttt ttgtttattt taccatacga agaggaaagt aggtgttatt
    27541 ccatgcaaac ttctcaaagt atatggcttg aaaattgatc aaaagagata gaaacacaaa
    27601 aaagagaatg caacaaatca atgtcgcatt cccttctgat atattttatt cagcacttat
    27661 tttgaacttt ctgaggtgct agctgatgaa cttgtggagc tggaagattc ttcttttgaa
    27721 gagttgcttg tgctactgga gctacttgag ctgctagaac tgtctgaaga gtctttttca
    27781 gaactagaag acgctggatc tggtccttta gagtaaatta cgttcacaac cattcctttg
    27841 gtaattgtcg ttcctgcccc tggtactgta cgaatcactt tatctttatc caccgtgttt
    27901 gaatattcgg aagtctcatg cccgatatat tctgcaccga cactagctaa ataactttgt
    27961 gcttcgcttt ttgtatagcc ggagatatca ttcaatttag gctctgttcc ttggcttacg
    28021 acaaaattga tgtaagtttc ttctgctaca acttcacccg aggctggatc ctgactgatg
    28081 atcgaacctg atggtacagt gtcgctatag tcataagtag cactaatatt attgatattg
    28141 aatccttgtg taacaagcca attacgaaca gtttcgtagt tttctcctac atgactactc
    28201 aaagtcactg tttctggtcc tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg
    28261 acttctgtat cttctgatgg agattgcgaa atgatcattc cgctttctac ctctgaactg
    28321 aattcctctt ctttttgaat caagtcagca gaaaacccta attctttcaa acttgcttcg
    28381 gcatcttcat aacttttacc actgtaatca tctaatttga tttttttcgt gccgctacta
    28441 atatacagag tgacttcgcg tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc
    28501 actacattac cttcttcgat tttgtcatct gcaatttctt ctgtttttgt tgctacttct
    28561 aatttcgctt ttgcgagtgt ttctctagca gaagcttcag aaagatcaga aacatcagga
    28621 acaacaactt ctcctcgccc gccagaagca aagaatacag ctgttccgat tcctaaaaca
    28681 gcaagaatag ctaataggat cagccaccat tttttctgtc ctttcttttt aggatcaggc
    28741 agaacttctt cctcctctgg taaggtttcg ttttctggct tgtcttttgg cagaggcata
    28801 gatttgaatg aatcaggcat tggtgcatct tccgtaaggg gtgtgataac ttttgtttca
    28861 ttatccatca catgaggctg ccatttttct tcgtttgcac gagctggtga taatactgta
    28921 gaaagatcat cagacatttc ctcggctgac ttataacggt ctgcaggttc tttagccgtt
    28981 gcccgtaaaa tcacgttttc cagtgcttgt ggcacatttg gatcaagtgc ttttatagat
    29041 ggaagatcat cttgaaaatg ttttaaagca attgttacag cagattcacc atcaaacggt
    29101 acgctacccg tcaacatctc ataaaggatg atccctaaag cataaatatc tgactgcttc
    29161 gttgccatac ttcctctggc ctgctcaggg gacagataat gaacagatcc tagcatcgta
    29221 ttcgtctgcg tgatcgatgt ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt
    29281 acaacacctt cattatcgat cagtacgttt tggggtttca aatctcggtg gatgatttga
    29341 tggttatgag ctaaagaaat agcagaaagg atctgctgca taatatttac tgctgtttca
    29401 taaggaacag gataatgtgt ttggatatat cgtttcaagt ccattccttt gacgtattcc
    29461 atcaccagat actgcatgtt gtcttcttca ccaacatcgt aaacactaac aatgtttgga
    29521 tgaacgagtt ctgttgctgc taaagcttct ctctgaaatc gtcgaatcgc agtctggtca
    29581 ttttgaaagt caaatcgtaa gactttaact gctacatcac gatccaagat taaatcgtgc
    29641 gccaaaaata catttgccat accaccgcta ccgatatttc cagtgatttg ataacggcca
    29701 tttaattttt tgcccaactc gatcatacac tgtcctcctt atattcgata agaagaacgg
    29761 tgatattatc cgctccgcct gcttcattgg cacgatcaat cagctcagtt actttatgag
    29821 acaatgggcc ttcttgattg atgatcgttc ctatttcttc ttcagacagc atgttcgtca
    29881 agccatctga acataacagc agtcgatctt ttagttgcca aagatgagta gaaacatcta
    29941 cttcgactgt tccaggcatg ccgacagaac gtgttaaaat atttttcctt ggatggttca
    30001 ctgccatttc ttcgctgatt tctcctgatt tgactagttc attgactaaa gaatgatcct
    30061 ccgtcaattg catgatttta tcttcacgaa tcaaataagc tctgctatcc ccaacattgg
    30121 caatcgtgaa ccagtcatct gacagagcgg ccgctacgat tgttgttccc ataccgttat
    30181 attccggctg ttcttgtcca cgctgataaa tcagcatatt ttcttcttga atcgattgaa
    30241 tgaaccattg ggcgattttt tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta
    30301 gattcgttac cgccatctga ctggcaatgt ctccggcacg atgaccaccc atcccgtctg
    30361 ctaatacagc aagcttgaga cctgaacggc tggtaaatac actggcatag tcttgatttg
    30421 tgttacgtcg tcttcctaca tctgattgat attcaatctg cattttttca cctcattact
    30481 ttttgcgcaa acaacaaatg aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca
    30541 acattttctc ttcgattgat gattgtagca taggattggt caaaacatca atcctctcaa
    30601 aatcagggtg tctttccaag aaagcagaaa caacgtcttg gttttcttct tttaagatcg
    30661 tacaagtgct ataagtcaga ataccggaag actttaaagt tggtgcgcag ctttctaaaa
    30721 tttccaattg gatttttggt aaattcgcta attcatccgg ctgtttttta taacgaatat
    30781 ctggttttcg ccgaagcagc ccaagacctg aacaaggcgc gtctactaaa atccgatcaa
    30841 actgctctgc ttcgaactgt ttactgactt ctctagcatc catcttttga gcgtagacag
    30901 cagaagagac acctagacgt gctgcattat cttcaatgag tttgactttg tgttcgtgta
    30961 catctaaagc gaccacttct ccaccgcatg acggatctaa aaaagaagca atatgcgtcg
    31021 tcttacctcc gggtgctgca caggcatcta agacatgatg ttcaggctcg atttgcatag
    31081 acggcgctac aagcatcgaa ctttcatctt ggatcgtcat gacgccatcg tggaacaatg
    31141 agctagaagc taaatgccct tttttcgcta ctataccgta tggactgact tggctttttt
    31201 cagctgtgat ctcttcttct tgcaaaacag tgatggcctg atctctggaa agtcggcgtg
    31261 tatctactcg ggcactagca tggctaggct gatatagcga taaaccgagc ttttcagttt
    31321 cttcgtatcc gatttgatcc accagtcgtt tcgtcatcca tagaggcagg ctgattttga
    31381 cagaaagtct ttcgatagga tcagcaatgc cttgcagatc tggtgctcct tgacgctgat
    31441 aattcctcaa gacaccattt acaaatttcc ctgtcccagg atttcctttt acttttgcga
    31501 tttcgaccgc ctcgtttaaa atagcatgtg cagggacttt atctaaaaat tctaattggt
    31561 atacagaaag ataaagaagc atcttgaccc agtcatccac tttttttgct ttagcgatca
    31621 atggtgcaac gtaaaattcc aatagtcgct gatggctaat cgtaccatag atcaattctg
    31681 taaataaacc cacatctttt tgatttagct cgctttgttt gatcatctca ttcaacaaaa
    31741 gattcgagta ggcgccaccc ttgttcactc gctctaatgt cagtaatgcc atatatcgta
    31801 ctgatttttt cattttttta gggatttttt tcattatttc acctgctgac caatctgaac
    31861 ttgctgaccg cttccgttaa ggaaatcatg tacggcctgt tttcctttac cagctggctg
    31921 taattcattg atggctaaga cggtctgttt accgcaagca atccatagat tttttttgtc
    31981 tttccggatg atcgtaccag gtgctttgtc tgtagtttct gccaatactt caaccgataa
    32041 taatttccag cgtatgccct cataggtcgt gaatgcaatt ggccaaggac gcattccgcg
    32101 tacttggttg tcaatttctt ctgcagtttt atgccaatcg attgcttctt gttcccgtgt
    32161 gatgtttggc gaaaaagttg cttctgactc gtcttgaggt actggttttt gtccatccaa
    32221 aatattgggc aatgtttcta gcaatagctt tcttcctaaa aggctaagct tttcaaacat
    32281 cgtccctaca tcatcttgtt tagtgattgg tatgctttct tgcgcataga ttccgcctgc
    32341 atccattttt ttgatcattt ccatgatcgt tacaccagtt tctttctccc cattcatgat
    32401 ggcataatga acaggcgcac ctccccgata tttaggaagt aaagaagcat ggacattgat
    32461 tgcccctaat tttggtactt gcaataactt ttcaggcaag aattggccaa atgccgcagt
    32521 gacgatcaca tcaggcacca attcaatgat ttcttccatt tctggcgaac cggagatttt
    32581 ctctggctgc agcacacgga taccgtgctt cagagccgct ttttttaccg gtgtcggcgt
    32641 aattactcgt ttccgcccaa ctggacggtc gggctgtgtt accactgcct ggatctcgta
    32701 acctgattct atcagccctt caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac
    32761 tagcttagtc atcaagatgt tcctccatat attcttcaag ttcttcttcc gggatctgct
    32821 gtatcatttt ctcaatgaac aagataccgt ctaagtgatc gatttcatgc tggaaggcac
    32881 gggctaaata gccaaaagcc gtcacttcta tctcttctcc gtctcgatca taataacgca
    32941 cggtcacttc atctgctcgc ttgaccgttc catagacatg cggaatactg agacaacctt
    33001 ctacgtcaag gctttcacct tttgcttcga tgatttctgg gttaatcagt tcaaatttct
    33061 cgccttcatc tacttcgatc acagcaattc tcttgttttg tcctacttga ggagcagcga
    33121 tccctattcc gtcattcgca atcattgtct catataaatt gtctaataaa gcgattgtct
    33181 cgtccgtgat tacatcgatt ggctgagcag ttctttttaa tttatcatta gggtggatca
    33241 atataggata gcgcataatt tggccctttc ttaaataaag tttaatggtt cgttgtcaat
    33301 tgccacaaaa agaccttgtc gctgttcttt ctggcttcca ttcaaaattt catccaaaac
    33361 atgaggaaga ttcggttcat gtttatattt gatgattagt tgataataat atcggttttt
    33421 cactctcaaa atcatgctgg gcgttggccc taatagcaag ctttgagggc ttaacacttc
    33481 ttttagctga ttggcaatct gaaagatttt ctttgccgct acttgttctt caggatggct
    33541 ggcggttatt ttcacagtaa agtaataagg aggatagcct ccacgatgcc gtacatgcat
    33601 ttcctgctga taaaactgct cataattttg ttttttagcc aattctatgg cgtaatgttg
    33661 aggattgaat gtttggatca cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga
    33721 aacttgggta agcaattgga atgtatgctc actagaacga aaatccggta aattcaggga
    33781 ggtatcagca ttcaacaccc caactaatgt cacttctgga aagtccaaac ctttagcaat
    33841 catttgtgtc cctaataata tatctgcttc ttttgcaccg aatttttgta aaatctgttc
    33901 gtgtgcccct tttcttcgag tggtatctac atccatacgc aaaatcctag cttgcggata
    33961 aagttctcgg agttcctctt ctactttttg agtacctgtt ccatagtagc ggattttatt
    34021 gccgccacaa ttaggacagc gattgggaat aggttcttca tgtccgcaat aatggcagcg
    34081 cattgaacgt gtatccatat gcagtgtaag agagatatcg caattaggac atggaagaac
    34141 aaatccacag tccctacaca tcacaaaaga cgaatatcct cgacgattga gcagtaggac
    34201 agtttgttct tttttgtcta atcgattttg tattttttcc tgcaaattag cagaaaaagt
    34261 agaggtccga tgattctgaa actcttcacg catatctacg atctcaatag ctggaagctg
    34321 tgcagctgct ttggctcggt ggtttaactg caataactga taaacgcctt tttgagctcg
    34381 tgctcttgat tctaaagagg gcgtcgcgct tcctagaact attgggcaat gatgatattt
    34441 actgcgccaa attgctagat ctctcgcatg ataacgcggt gtctcatctt gtttgtaact
    34501 tgattcatgt tcttcatcga taatgatcag cccgatattt ttcagcgggg caaaaatagc
    34561 agatcgtgcg cctactacta cttgcgcttc ttcccgctca atcttgcgcc attcatcata
    34621 tttttcacct tgggaaagtc cgctatgtaa gaccgcaact gattctccga agcgtccttt
    34681 gaatcgctcg accatttgcg gagtcaatgc gatttcaggc actaacataa tagccgtttt
    34741 attttctgac aaaacatcag cgattgcctg aagatatact tctgttttcc cgctgccagt
    34801 gattccctcc agcaaaaaga cctgatcttt ttgctgctga cctgctgtga tgatttgttc
    34861 gacagcattt ttttgttctg cattcagttc caatgccgtc gtttggtcaa atactcggtc
    34921 tttataaggg tcgcgataac gttctgattc tataaactcc agccagccct tttcttttcc
    34981 ttgattcaag acagcagtac tgattccttt atttttgaaa taagcaattg gttcttcttg
    35041 gctcagttca gataaacaca aaagaagttc tttttgtttc ttggcatttt gccgtaattt
    35101 tgcccattct tcttcgattt gcttttcagt taacaaagaa cggaccaaac gaatcgtttt
    35161 tactttattt cgtgtatgga cttcatattt gatatctact aattgctctt ggcgccagcg
    35221 aatcaattga gaaaggctgc cttcttcttc tgcttccttc caggaaatca cttctcgctg
    35281 atcaaacaat acctgaactt ctggtgtgtc tgccaaagga tagatcaatt tatcatattc
    35341 tgctttcatc acactcggaa gcatcgtttg tagacaagtg attttgaatg cgtaagtaat
    35401 ttttttcata taatcagcta atgaaagtaa ttcttcattg accaccggag caaggtctag
    35461 caaacgaata actgccttca aagatggatt agctgattct tctgattttt ggatagccat
    35521 aacaaatcct tggacatgac gattgccatt accaaaaggg acttctactc gcatcccgac
    35581 ttgcaaagct gtttccattt cagagggaac caaataagtg aatggctgat cggtctgcat
    35641 agtaggtacg tccacaatga cttcagctgt taatgtcatg gttcgtttcc tccttttttt
    35701 gtcttttctt atcaagcgac aaaaagttcc ttgcctattt tactagagat cgatggattt
    35761 gtcttatctt ctgccaaatc ctaacaaaaa attaacagca gataagaaaa aaaagaactg
    35821 tgtcaaaaag ttcagacaca gccttttcta ccagacaata agtggcgaat gtttgaaaag
    35881 gaaacagctg ggtttgcttt tctttttaga tgttcgctct atttttcttt attacatttt
    35941 ttgctcttca cggatacgtg attccaattc gtgttgttca cgctcttttt gtgcttgacg
    36001 ttcctcttct tccatcttca aacgttcacg ttttaattca ggatgtggat cattgactac
    36061 atttcctgca tcgatttctt ctaaagcttg accgacgctt tttactgaat caaaagaatc
    36121 tagagtaggc tgtgcacttg catccaattc atgcgcgcgt ttacttgcta aaatcactag
    36181 agaatatttt gaatttacac gatctaatag tgagtcaatt gaaggtttta acatcataag
    36241 actacatctc ctttaacatt ttaatatatt taccgattac gcgatctact cgaaaatgtt
    36301 cactggcaat aatgtttttg attcgttcaa ctgctagcgg cacttcatca tttacaacag
    36361 catagtcgta aagagccatc atttcgattt cttcttttgc aactttcatt cgtttttcga
    36421 ttacttcatc tgcatctgtt cctcggccta cgatccgtga ttttaattcc gctaaatctg
    36481 gcggagtcaa aaagatgaat acaccatcgg gcactttttc tttgacttgt tttgctcctt
    36541 ggacttcaat ctccaagaaa acatcttttc cttcatccaa tgtcttgttg acatatgaca
    36601 aaggagttcc ataataattc ccgacatatt ctgcgtattc cagcatctct ccagcttcga
    36661 tcattgattc aaactcttct ttgcttcgga agaaataatc aacgccttcg acctcccctt
    36721 cacgcatttt acgggtagtc attgagatcg aatactgaaa atcattatcc tcactctcaa
    36781 aaattgcttt tcgtactgtg ccttttccta caccggatgg accagacaac acgattaata
    36841 atccacgctc tgacatgacg acgtcctttc aactagtctt tttgtactta tcgtttattt
    36901 tgcacttttt ttctgcaact ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa
    36961 agctaccctg taccaagagg tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc
    37021 aaacataaac agcttctcat gtttttcgtt tggttggggt tattatcgca gatgcaagct
    37081 tttgaacgcc gtgtataaga ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa
    37141 ttttgtgaaa atagtttctc acatttttgc ataaatttgg cttaataccg agaaaatggt
    37201 ttttgaacat cacttattca aaataaaaaa agattgtgac acgaattttg tcacaacctt
    37261 taaatgtatt cctagagcaa agaagctaag cgaagaaatc caattctgtt tatttattgt
    37321 atttctttgt tcagcatcct tgctaaaagc ccatattgga caaagtattc actacaccca
    37381 tgatctcatc ttttcgagaa cgtgcttttt ctgtcggatc ttctacctct tctgtgtcaa
    37441 ttcgaacaat aaatttccga ttcatcgtca cgtaagcacc gtcgttgatc aaaataaaca
    37501 gttcgttctc attatagtct gcacaggcat ctttccatac gtcaaagccg tttttttcag
    37561 aagggatatc ccgtttaacc acagtttttc cacttaattc gtggaacgtt acgtcataat
    37621 atttcattat ctctacctct ttcgtcattc aatattttgt acttgttctc tgattttttc
    37681 taagatcgtc ttagactgga cgacttgatt tttaatttcg attgcatttg atttggaacc
    37741 gatcgtgttg atttcccgat tcatttcttg gatcaggaaa tctaattccc gacctacagg
    37801 acattgtgct tcgataagtt catctagttt ttgtacatgg atcgccagac gatccagttc
    37861 ctcgtgaata tctccgcgtt ccaacaagat ggctagctcc gtcaacaacc gttgctcatc
    37921 gactttcgct ccaagaaatt cctccagctt tttttgatac cttatttgaa attccttttc
    37981 atagatttct acaaaactat cgagtttttt tagtacttct ttgaattctt cagtattctt
    38041 tttcaacact aaagctaaag cttgaccttc tttttctcga ctcgcctcaa tagaatcaag
    38101 tgcgcttttc aatgtatcta gagctaactg tgccaattct tccatgctct cttccggttt
    38161 ttcttctacc gttacgaagt tttcgtccag cacgaagcgt tccgccatcc gactaattga
    38221 cacttgattt tgactaccgt atcgttgttg aattccgtct gtcaattgag tgataagtga
    38281 atcaatcaat tcccagttga tgacgatctg tttttcgttt ttgccaatat aagctagatt
    38341 cacaaatact tcgattcgac cgcggcttaa ctgctgttta ataagctgac gaaaatcgtt
    38401 ttcaacaaag ttcaggattt taggacttcg tatttgaaca tctagaaaac gctggttgac
    38461 actttttatt tcaatatcta actgatacgc ttttgtttcg cggatggctt tgccaaaacc
    38521 agtcatactt ttcattcgac agggtccttc ctcattacat attttctatt gctttttgca
    38581 gttgttcaaa ctcttcgttc gtcaatgtca ggcctttccc cattttttca tggtctggcg
    38641 cccaatctcg aagatcgaat tttggcgggc gaccattcca gctgactagg ttcagttctt
    38701 tacgccaccc cttgttgttt tctgacaata tagcgatttc ttctacgatt tcatatgaaa
    38761 attcttgtgc catggttgtt cctccttgag ataatataaa tataggatca gtaatgtgtc
    38821 aactggttgc ttaagcaatt catgctgcaa acgatccttg cgttttcctt gtagcagata
    38881 atcaaaataa acagttttcg tgcgctcatt ttgtattttt tgctggtatt ttcgatacct
    38941 tcgccattct tcagtactta attgaccaat ttgtgccaat aaatcatccg gcatactttt
    39001 tatatcttgt tggcagatca tttgaatcat catgtctact tgatagattt cattgggcag
    39061 taaaaaatgt ttttttgcta aaagttctag catcgccaat ccgcatgtct ctggtacgac
    39121 cctgttctta tataaattac gcagtttttg ccaaaatttt atgcttttga aaaaagataa
    39181 ttctaactga atttcttttg ttaagtaaga caaaaatcct cgccagtatg ttcttgcagg
    39241 tttaacagaa tgaaatgtat accagttccc atagtaatag cctaatcgtt ttttcatttc
    39301 cagatagcct aattgtaaat ttaattggtt cctttggcta tcaaaaagca gaaagctgcc
    39361 tagtgtccac aatgtttgac atttccaatg gataaaaatg tctggcatac gaattttttt
    39421 cgctgggcct ggcccttgca catctacgac aaatgcctct gttgctcctt tgttaatcgc
    39481 aatatcaata ggtattttgt tgcgataccc accgtctgca tatttatttt ttccgatctt
    39541 tcgataagcc atagcgggat aaaatgaagc agaggcgagt atccaatctg gtatttcttc
    39601 tgtttttgtc tgttggatat cagtcactac ttccgctagt tctggcagtt tagtagacac
    39661 cgtatataaa cgtattttgg gattatgttt gagtaaggac aaatccaaat tgtctttgac
    39721 catcttttcc aatggagcag aagaaacccc gccttcaatt agtgctgttt tcgtcatttg
    39781 gcgtctttct cgatagaagc gttcacgaag gtcttcaact gcagccattt ctggtaacgc
    39841 aagcacttgt cttgtcgaca gctttttcca taaattcaca gcttttttca tatctccttg
    39901 taaaatcaat gcgccgttca acgcacctac cgaggtacct gtgataatct caaagttgat
    39961 atcatgttcc ttcagcgcct gccacacacc aatctgataa gcaccgtgcg ctcctcctcc
    40021 acctaaaaca agtgctgtat gatagaccag ctttttattt aattgtgtgg ttccttgata
    40081 gccgttttta tgaaaaatac gcaaccattc ttctgataga ggagattgaa ataaaaaaca
    40141 acagttttct ttaataaaaa aacgagcgca agcttccata taccgaaaaa attttttcca
    40201 ttcaaaagtt tcaccttcaa tcaacagatt attaaaaata acatctcctc tacttttagc
    40261 aatagtaaaa tagacggtat attttttcga tactgcttta aaagcacatt gcttttttgc
    40321 agaacgccaa aaagcgtctc tactttcttg atgactttga aaaaatggaa ggggttgttt
    40381 tgcttgctgg ataggcgaat acagcgacat tttctgtgaa aagtaaactt tttttatctc
    40441 catcgcgctc tcctttttct tcaagtatac cagtacgtca taaaaaggtc atgagtctcg
    40501 tattcgactt tgtagattcc ataagaaaac aattcataaa acctccttta aataaaaaaa
    40561 tacaaaaatc acaaaaatta attgaaaatg agagtgtttt caatgaaaac gtgattttgt
    40621 aatg
//
LOCUS       DASJUF010000012        37136 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJUF010000012 DASJUF010000000
VERSION     DASJUF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.012"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..384)
                     /locus_tag="U4B62_000577"
     CDS             complement(<1..384)
                     /locus_tag="U4B62_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140759.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="U4B62_000578"
     CDS             complement(569..967)
                     /locus_tag="U4B62_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN2140760.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="U4B62_000579"
     CDS             complement(964..1737)
                     /locus_tag="U4B62_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2140761.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="U4B62_000580"
     CDS             complement(1734..2417)
                     /locus_tag="U4B62_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2140762.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="U4B62_000581"
     CDS             complement(2584..3471)
                     /locus_tag="U4B62_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2140763.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="U4B62_000582"
     CDS             complement(3468..4337)
                     /locus_tag="U4B62_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2140764.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="U4B62_000583"
     CDS             complement(4474..5760)
                     /locus_tag="U4B62_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2140765.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="U4B62_000584"
     CDS             6033..6611
                     /locus_tag="U4B62_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2140766.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="U4B62_000585"
     CDS             6611..7540
                     /locus_tag="U4B62_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2140767.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="U4B62_000586"
     CDS             7537..9273
                     /locus_tag="U4B62_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2140768.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="U4B62_000587"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="U4B62_000587"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN2140769.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="U4B62_000588"
     CDS             complement(10169..10678)
                     /locus_tag="U4B62_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN2140770.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="U4B62_000589"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="U4B62_000589"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN2140771.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="U4B62_000590"
     CDS             complement(11979..13070)
                     /locus_tag="U4B62_000590"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN2140772.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="U4B62_000591"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="U4B62_000591"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN2140773.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="U4B62_000592"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="U4B62_000592"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN2140774.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="U4B62_000593"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="U4B62_000593"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN2140775.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17033..18007)
                     /locus_tag="U4B62_000594"
     CDS             complement(17033..18007)
                     /locus_tag="U4B62_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN2140776.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17988..19871)
                     /locus_tag="U4B62_000595"
     CDS             complement(17988..19871)
                     /locus_tag="U4B62_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN2140777.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19889..22114)
                     /locus_tag="U4B62_000596"
     CDS             complement(19889..22114)
                     /locus_tag="U4B62_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2140778.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22229..23263
                     /locus_tag="U4B62_000597"
     CDS             22229..23263
                     /locus_tag="U4B62_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2140779.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23330..24343)
                     /locus_tag="U4B62_000598"
     CDS             complement(23330..24343)
                     /locus_tag="U4B62_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2140780.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24421..24609)
                     /locus_tag="U4B62_000599"
     CDS             complement(24421..24609)
                     /locus_tag="U4B62_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN2140781.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25049..25177)
                     /locus_tag="U4B62_000600"
     CDS             complement(25049..25177)
                     /locus_tag="U4B62_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2140782.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="U4B62_000601"
     CDS             complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="U4B62_000601"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2140783.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26664..27719)
                     /locus_tag="U4B62_000602"
     CDS             complement(26664..27719)
                     /locus_tag="U4B62_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2140784.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="U4B62_000603"
     CDS             complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="U4B62_000603"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN2140785.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="U4B62_000604"
     CDS             complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="U4B62_000604"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN2140786.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="U4B62_000605"
     CDS             complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="U4B62_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN2140787.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="U4B62_000606"
     CDS             complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="U4B62_000606"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN2140788.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="U4B62_000607"
     CDS             complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="U4B62_000607"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN2140789.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35481..37040
                     /locus_tag="U4B62_000608"
     CDS             35481..37040
                     /locus_tag="U4B62_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN2140790.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10262 a         8064 c         6697 g        12113 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag
    16801 aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg aaactttttt
    16861 taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt attgaagaaa
    16921 atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat
    16981 aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa
    17041 taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg
    17101 ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc
    17161 gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg
    17221 gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt
    17281 gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca
    17341 tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat
    17401 aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat
    17461 actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg
    17521 gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt
    17581 ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta aaaatgattt
    17641 caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat
    17701 cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc
    17761 ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc
    17821 gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt
    17881 ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg
    17941 tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat
    18001 ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg
    18061 atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc
    18121 atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt aaaatcccat
    18181 atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca
    18241 cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt ttccgcttta
    18301 ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa
    18361 atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg
    18421 tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata agattcattt
    18481 ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct
    18541 cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca
    18601 aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg
    18661 attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt
    18721 acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg
    18781 atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt
    18841 tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag
    18901 tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa
    18961 ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt
    19021 tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct
    19081 acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca
    19141 ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct
    19201 cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc
    19261 aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa
    19321 taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga
    19381 ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga gatttgtttt
    19441 ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta
    19501 gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga
    19561 aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct
    19621 aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta
    19681 taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca tgatccattt
    19741 aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata acaaacatga
    19801 tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata
    19861 tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt
    19921 ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa
    19981 atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata
    20041 cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat
    20101 cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac
    20161 gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt
    20221 ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa ccaaaaacag
    20281 cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg
    20341 gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt gttccatatt
    20401 ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat
    20461 aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg
    20521 gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta
    20581 cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca
    20641 tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa
    20701 tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag
    20761 gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt tcagaatcaa
    20821 ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta tgagcataat
    20881 ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg
    20941 aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc
    21001 ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag
    21061 tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga cctcttgcga
    21121 ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc tgacttgagt
    21181 aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc cacgaaaaac
    21241 gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt tcaagttcga
    21301 aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta ttttctgtcg
    21361 tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt cctcgacggc
    21421 tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt tcattgacat
    21481 gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag tctagctgga
    21541 aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc tttacagatc
    21601 gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa atcgctgttt
    21661 ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc acataagttt
    21721 gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca tatctcagat
    21781 ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc
    21841 tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg tcaggattta
    21901 agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg tctttgtact
    21961 gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct atctttatca
    22021 gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca aataagatga
    22081 aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca aaacggaaac
    22141 cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt caataaaaat
    22201 tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc
    22261 aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga tccatgtggc
    22321 tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg acatcagtac
    22381 tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga tcggcaaagc
    22441 gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag aagaagctcg
    22501 acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta aacaatggga
    22561 agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag aatcgattac
    22621 cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga
    22681 ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg aattgttctt
    22741 agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata tggatgaatt
    22801 cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc attttatgat
    22861 cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag aaaaacaagg
    22921 tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg ccattcttgt
    22981 cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat
    23041 tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa acctctcccc
    23101 tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg ttcgaatggc
    23161 attggagcga attgacggta tacgggacga accaatcatt ttgacttatc ctgcacgatt
    23221 gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa
    23281 gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact
    23341 tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa
    23401 attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta
    23461 cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat
    23521 ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt
    23581 ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt
    23641 tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa
    23701 caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt
    23761 tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg
    23821 aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt
    23881 cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg
    23941 aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt
    24001 ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc
    24061 aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct
    24121 ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat
    24181 cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg
    24241 ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga
    24301 ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt
    24361 tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat
    24421 ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg
    24481 ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca
    24541 caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg
    24601 ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag
    24661 agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg
    24721 cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg
    24781 ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga
    24841 atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa cgaaaaatct
    24901 gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa cagtccttat
    24961 tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat aacaaaagtt
    25021 aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg ccttttctca
    25081 tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca ccttgtttct
    25141 ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt taacgaatga
    25201 agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa ctttcttcac
    25261 ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg gattacatga
    25321 gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat gatgatctaa
    25381 tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt cttgccattt
    25441 tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt tattatttaa
    25501 agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat acaccttatc
    25561 cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt cgatcacaat
    25621 gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact ctgcttgttg
    25681 agtatttact tgataaaatg actttgctga gattcgatag gtttttccta atagctgatc
    25741 ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca catttgtttt
    25801 ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat gaatagcttc
    25861 agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga ccatcatttc
    25921 atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat ttttttcttc
    25981 attatatcct cgtacttgga accgttgcaa gatatctctg actactttga ttgcttgatc
    26041 gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc tattcttttt
    26101 gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg ctttattacg
    26161 gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg gcatcttagc
    26221 aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt gttcatactt
    26281 caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta caggctgacg
    26341 atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat tgcctgcttt
    26401 tactactagt atttctactt cttctccagg gagtgcattt tcgataaata atggataacc
    26461 atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga ctgtatatcg
    26521 ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc ctatttttca
    26581 atcctactga actctactat aaatcagcta attacacaac taaaaccggc actgacctta
    26641 ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc tgctattttt
    26701 ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa ttctttgctg
    26761 acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat ctgatcggta
    26821 ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg
    26881 tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt ttcaacgaac
    26941 aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc gccattcagc
    27001 atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat gattaaagaa
    27061 aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc aacagagttc
    27121 gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc atacttcaag
    27181 cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag ataagcaaga
    27241 taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt caaatagcca
    27301 cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat ttttacggtt
    27361 tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat ttttaatgct
    27421 cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg gtgttcaagc
    27481 ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc cactactaga
    27541 tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg ttctggtgta
    27601 gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat atccgccaaa
    27661 ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt
    27721 ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac accaggattt
    27781 gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc acgatctttt
    27841 ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt aatgattggc
    27901 agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac ttcttgggct
    27961 tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga gctgatcgtt
    28021 ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt tgtctcagct
    28081 aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca gttagatgct
    28141 tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta
    28201 agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata gcgttcgcga
    28261 cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc gtcatcgatc
    28321 acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc ttctttgaca
    28381 cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct
    28441 cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg
    28501 aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc ttgtccataa
    28561 gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt cacatcgctg
    28621 acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc ttcttctggt
    28681 gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca
    28741 acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc taaatggaca
    28801 cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca tccgtcatga
    28861 ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg gtaaaagtaa
    28921 tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa agctgctttc
    28981 atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata accccagtcg
    29041 atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg agagaaaatt
    29101 tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc aaaattcatt
    29161 cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct tgttcaaacg
    29221 catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc tgcaatccaa
    29281 ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca gccaagttga
    29341 cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga
    29401 ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct gcaaagactt
    29461 tttcaaaatc tcgtttgatc aatgtacgga cttgtcctgc ttttttgaag taagcgtcat
    29521 aataacccgc gcttaaggag aatgtaccta acatgatacg gcgttttact tcttcaccga
    29581 atccttctga acgtgagttg acatatacat catctaatga tttcacattt tcagagcgat
    29641 aaccgtagcg gataccatcg aatcgttgca aattggaact tgcttctgat gaggcaatga
    29701 tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc acaacagcac
    29761 ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca ggggccacac
    29821 cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc
    29881 cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg tcataaccgc
    29941 tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt ccgatttggt
    30001 caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa gtaggtttca
    30061 tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta tcactaccta
    30121 aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa gagccaccag
    30181 gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt tctgtacttc
    30241 cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct gcttgataga
    30301 ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc ttagatgcag
    30361 cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga ataccagcaa
    30421 ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc aatgcttttt
    30481 cttcgttcaa tgtaatgaat gcctcgacct caggctctgt ttctttgatt cgttcaaatg
    30541 ttgtttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga tgcagttctt
    30601 ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat cgatgattgc
    30661 gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt catcgcggtt
    30721 ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca cattcgacgt
    30781 aaatggaaca ccttccgtat ctacttcttc cagcatttca accatatcga tgattttacc
    30841 taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg ctaatttggc
    30901 aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta aacgagttaa
    30961 aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta ttgaaatttg
    31021 tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg catctgccat
    31081 ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct tgctgccagc
    31141 atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca ctttcccgcc
    31201 aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt taccagtaag
    31261 gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat ttcgaagccc
    31321 tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat ggacttcttc
    31381 gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat caagcgcaat
    31441 aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat
    31501 ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc gctcgaccga
    31561 attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt cttttatttt
    31621 gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat ctgctactag
    31681 ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat tcatcgcatt
    31741 tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag gattgatgca
    31801 ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg gacaatgtgt
    31861 aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt gcgaaacttc
    31921 tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga tatccttcaa
    31981 cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg ctactcggac
    32041 tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc attcgatgtt
    32101 ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc atctcggtgc
    32161 tttgatagta aaaccaagtt gatcttgtag cgagaattca ttcactttga tgacgattcc
    32221 atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt atgaccacac
    32281 ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa ttttttctag
    32341 ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag ctactgtgta
    32401 taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac gcaaagaacc
    32461 agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc tgtcttggtt
    32521 caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga tgttcattgg
    32581 ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga tgttttctcc
    32641 tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat aacgcaaaga
    32701 aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc ccactgcttt
    32761 ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta
    32821 aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc ctactcgttg
    32881 agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcgattgcta ctaattctgc
    32941 gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt agtattcgcg
    33001 agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt
    33061 catttcatgt tccatcattt accctccact actaagcttt ttcaattggc gcaaaggcag
    33121 ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga tccttcgacg
    33181 tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact ttatctccag
    33241 gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc gctgtctctg
    33301 gctgtttata gctatatgct gtttttttag cgaatacagg cgttttttta ggcgtaggac
    33361 gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga gaaggtcggt
    33421 tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac aacgcttctt
    33481 ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ctccagttcg ctttcttcca
    33541 tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat acaactggaa
    33601 attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta
    33661 aattgtcaat gtcggaaaca agtgccagat catttaagaa gaccgttagt ttttcttctg
    33721 gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg
    33781 ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt tggattttta
    33841 agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc actgtcaagt
    33901 atgggatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat tgctgtcctg
    33961 cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt ggccattcat
    34021 gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct cgtttgggcg
    34081 aattaacgat acgctcaaaa ctgattgaat cttgaggatt taccagcaca ttcaaatacg
    34141 ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc attttataag
    34201 gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca
    34261 agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc atccggtcta
    34321 cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc ttgtttcctt
    34381 ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca atgacttggt
    34441 ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa attactgcag
    34501 catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg cgccagccgt
    34561 aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct gccaataaat
    34621 tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga atataatgga
    34681 atttgttttg ataatacgtg agtgaatcag gatgttcttc aaataatcga atcgtattca
    34741 tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg taagcagcat
    34801 aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg
    34861 tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga tcatattttt
    34921 tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat
    34981 cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt aaaatacgca
    35041 cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg agaatcgcat
    35101 tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct aggatgttcc
    35161 aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct
    35221 ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga acagcttctt
    35281 tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac aacatccttt
    35341 cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta gctagttcaa
    35401 aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg cgctaaatta
    35461 aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta gataagactc
    35521 agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc gtcgttagcc
    35581 tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat gaaatgagtc
    35641 gggaacagcc gatttactta attggcctag ctatttgctg catccttgca gcatttttcg
    35701 tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca caagtagtgg
    35761 gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa aagttcattg
    35821 gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct tctatacagc
    35881 ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct tcagaagaaa
    35941 agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat gctcctatcg
    36001 caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt gtttggctga
    36061 catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc ggcttgagac
    36121 ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt tccttagtga
    36181 tcttaggagt acttctaggc atcttaggtt ttatttatca gaatgtcttg ctggctttgc
    36241 caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt cctttcttgc
    36301 tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat caaatcgttt
    36361 tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt gttcttcgat
    36421 tcgttttttc catgatttct tacggtgggg gtcttccagg ggggattttc cttccgatat
    36481 tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat ttcgggatgg
    36541 atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc acagcgattg
    36601 gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct cttaatcatc
    36661 tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt ctcggcggta
    36721 atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc gcctcactaa
    36781 aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta gacggattaa
    36841 tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc agaggaaatc
    36901 aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg gttatcttga
    36961 ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct tctatcacaa
    37021 atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact attcatctac
    37081 actacttgat tctatatgat tctataaaaa aagaagaggc tgggaaaacc cagcct
//
LOCUS       DASJUF010000013        36780 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJUF010000013 DASJUF010000000
VERSION     DASJUF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.013"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            159..3437
                     /locus_tag="U4B62_000609"
     CDS             159..3437
                     /locus_tag="U4B62_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN2140791.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3978..5885)
                     /locus_tag="U4B62_000610"
     CDS             complement(3978..5885)
                     /locus_tag="U4B62_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN2140792.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6015..6890
                     /locus_tag="U4B62_000611"
     CDS             6015..6890
                     /locus_tag="U4B62_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN2140793.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6970..7944
                     /locus_tag="U4B62_000612"
     CDS             6970..7944
                     /locus_tag="U4B62_000612"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2140794.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8124..9269
                     /locus_tag="U4B62_000613"
     CDS             8124..9269
                     /locus_tag="U4B62_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2140795.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9406..9750
                     /locus_tag="U4B62_000614"
     CDS             9406..9750
                     /locus_tag="U4B62_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN2140796.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10021..11307
                     /gene="efmA"
                     /locus_tag="U4B62_000615"
     CDS             10021..11307
                     /gene="efmA"
                     /locus_tag="U4B62_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN2140797.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11445..12044)
                     /locus_tag="U4B62_000616"
     CDS             complement(11445..12044)
                     /locus_tag="U4B62_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN2140798.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12448..13569
                     /gene="mnmA"
                     /locus_tag="U4B62_000617"
     CDS             12448..13569
                     /gene="mnmA"
                     /locus_tag="U4B62_000617"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN2140799.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13815..15236
                     /gene="arcD"
                     /locus_tag="U4B62_000618"
     CDS             13815..15236
                     /gene="arcD"
                     /locus_tag="U4B62_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN2140800.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15250..16368
                     /locus_tag="U4B62_000619"
     CDS             15250..16368
                     /locus_tag="U4B62_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN2140801.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16428..17720)
                     /locus_tag="U4B62_000620"
     CDS             complement(16428..17720)
                     /locus_tag="U4B62_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2140802.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17852..18430
                     /locus_tag="U4B62_000621"
     CDS             17852..18430
                     /locus_tag="U4B62_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2140803.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18559..18936
                     /locus_tag="U4B62_000622"
     CDS             18559..18936
                     /locus_tag="U4B62_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN2140804.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18869..20941
                     /locus_tag="U4B62_000623"
     CDS             18869..20941
                     /locus_tag="U4B62_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2140805.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21152..22099)
                     /locus_tag="U4B62_000624"
     CDS             complement(21152..22099)
                     /locus_tag="U4B62_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2140806.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22345..22809
                     /locus_tag="U4B62_000625"
     CDS             22345..22809
                     /locus_tag="U4B62_000625"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN2140807.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22871..23914)
                     /locus_tag="U4B62_000626"
     CDS             complement(22871..23914)
                     /locus_tag="U4B62_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN2140808.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24291..25256)
                     /locus_tag="U4B62_000627"
     CDS             complement(24291..25256)
                     /locus_tag="U4B62_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN2140809.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25490..26335
                     /locus_tag="U4B62_000628"
     CDS             25490..26335
                     /locus_tag="U4B62_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2140810.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26526..27713
                     /locus_tag="U4B62_000629"
     CDS             26526..27713
                     /locus_tag="U4B62_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2140811.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27719..28075
                     /locus_tag="U4B62_000630"
     CDS             27719..28075
                     /locus_tag="U4B62_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN2140812.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28077..28415
                     /locus_tag="U4B62_000631"
     CDS             28077..28415
                     /locus_tag="U4B62_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN2140813.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28491..28747
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28810..29844
                     /locus_tag="U4B62_000632"
     CDS             28810..29844
                     /locus_tag="U4B62_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2140814.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29837..30523
                     /locus_tag="U4B62_000633"
     CDS             29837..30523
                     /locus_tag="U4B62_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140815.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30547..31395
                     /locus_tag="U4B62_000634"
     CDS             30547..31395
                     /locus_tag="U4B62_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2140816.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31592..32365
                     /gene="sufC"
                     /locus_tag="U4B62_000635"
     CDS             31592..32365
                     /gene="sufC"
                     /locus_tag="U4B62_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN2140817.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32378..33664
                     /gene="sufD"
                     /locus_tag="U4B62_000636"
     CDS             32378..33664
                     /gene="sufD"
                     /locus_tag="U4B62_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN2140818.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33661..34896
                     /locus_tag="U4B62_000637"
     CDS             33661..34896
                     /locus_tag="U4B62_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2140819.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34883..35353
                     /locus_tag="U4B62_000638"
     CDS             34883..35353
                     /locus_tag="U4B62_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN2140820.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35358..36749
                     /gene="sufB"
                     /locus_tag="U4B62_000639"
     CDS             35358..36749
                     /gene="sufB"
                     /locus_tag="U4B62_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN2140821.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        12021 a         6791 c         7870 g        10098 t
ORIGIN      
        1 tttttccgtt aataaatctc tttattatca acaacttaag ttatattttt tctttttata
       61 aaatacactt attataattt tgttttttat tgacagtaaa aataaattag gtaatacttt
      121 aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa cgtataaaat
      181 acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt tctgtcgttg
      241 aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg aacattgaag
      301 ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg gaagaaggaa
      361 atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct gaagaagcaa
      421 atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg tatgccattg
      481 aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa ttccagtcaa
      541 gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc aactttagga
      601 tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa ggatttgctt
      661 taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt tattcaaatt
      721 catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga caagaagtgg
      781 tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg gcaatcacag
      841 gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca gtggaaacaa
      901 cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat aatatattcg
      961 gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg gacgtagaga
     1021 tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca ggagaagaaa
     1081 tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa tcggttattt
     1141 atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc gaagttgctt
     1201 actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg attgatccga
     1261 tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg tcctttagag
     1321 caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg cttaagaacg
     1381 caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa ctgatggaag
     1441 gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca gaagaatacg
     1501 tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac cgtaaaccta
     1561 aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga agcagacaaa
     1621 atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca atcgtcatgg
     1681 cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt aacgggacaa
     1741 gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt cctgtacttg
     1801 attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt ttacgagggt
     1861 ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga aatcataatt
     1921 tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc agcggtagct
     1981 caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt acatcttttg
     2041 aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg acaataggag
     2101 aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt tgggaccttt
     2161 ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca gttgcttatc
     2221 gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc aaaatgggcg
     2281 gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa aatcttgcga
     2341 aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca agttttaata
     2401 acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac taccatgctt
     2461 ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc cgcccattga
     2521 atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa cgagagaaac
     2581 aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct ctaaatacat
     2641 gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc catgttcatg
     2701 gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg gatggtcttc
     2761 cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa tatccagagt
     2821 atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat ccgattatta
     2881 tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa ctgaagcctt
     2941 ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt gaagatatgc
     3001 gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa ttttctaatc
     3061 ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc acttatcgtt
     3121 cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc cagcaaaaag
     3181 cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca gaaagagaaa
     3241 atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat ccggatagga
     3301 cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata
     3361 acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga
     3421 aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa
     3481 agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga
     3541 tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag gacagctttt
     3601 tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac
     3661 ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc aggacgctga
     3721 tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta agtcaaattt
     3781 atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa actaagcagc
     3841 tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa ataagccgaa
     3901 atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt gaagctgacc
     3961 acttctggca caacctctta ccagattcgc actctttttt ctggcgctag atacatagcg
     4021 tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg ttgcacgttt
     4081 gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg gtattctttt
     4141 tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat agaaagatct
     4201 gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc aatattttcc
     4261 gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc gaattcttcg
     4321 atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca ccagttgttc
     4381 aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga aatctcatga
     4441 gcgataacag ctccgattcc accataatta gcagagcttg attgttctag actgtaaaat
     4501 ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg ataataagca
     4561 ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt ccatttgctg
     4621 aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa agtaccaccc
     4681 tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc ggggtagcct
     4741 acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc atccccaagc
     4801 cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac catttgttct
     4861 acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc aactacttga
     4921 tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc ttcgtctgtt
     4981 ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga tagataacca
     5041 ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa atgagcttct
     5101 gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac tttatctgga
     5161 gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt gtatttaata
     5221 aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc ttctgcactt
     5281 tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat tgcttctgcc
     5341 tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa gaaaacgcct
     5401 attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg aaggaataaa
     5461 gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc atctacatcc
     5521 agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt attcaattcg
     5581 tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg agcatgcaac
     5641 tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg tttcattttt
     5701 tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat caaaagttgg
     5761 tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc agggattttt
     5821 gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa tcgttctttg
     5881 ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa aatgaaggaa
     5941 ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt gtttttttca
     6001 atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta gtggataaac
     6061 ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag cagctgcagt
     6121 caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt tatgtgatta
     6181 tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat caagagggtc
     6241 tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct agatggatca
     6301 acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag gaactcgcaa
     6361 attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg gctaccccct
     6421 caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat caagactatc
     6481 gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat tcgttggttt
     6541 tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt ccttttcagg
     6601 ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt gcacgagcaa
     6661 ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa cctgtgatga
     6721 ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca gcgtacgctt
     6781 attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg ggcgattatc
     6841 ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa gaatttgctg
     6901 aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag aagttttggg
     6961 gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag tttgaatggc
     7021 aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg taaaagcgtc
     7081 gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat tcgaggggat
     7141 catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat ggaactactg
     7201 atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt attgccttac
     7261 tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac tgcgaaattg
     7321 atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga tctgcacacg
     7381 gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat gccgttgttt
     7441 gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt ttcgccaaaa
     7501 aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac attagccatc
     7561 gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga tgttcaagga
     7621 aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc acgagcagca
     7681 aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca tggattattg
     7741 tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg tatcactgat
     7801 tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac atgttcagaa
     7861 ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc gttattccgt
     7921 ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt gacagaagtg
     7981 agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct atgcccatga
     8041 aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta tgctagaatg
     8101 tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg gatcatgcgg
     8161 caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg caagacacgt
     8221 ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa ttggaaacag
     8281 caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc tttaacagtg
     8341 ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt caaaaagaag
     8401 gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc atgaaatatt
     8461 tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg aatctgtcga
     8521 tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg atgtacggga
     8581 acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta gcggatcatc
     8641 cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc catccagaag
     8701 aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca aaaggcgtcg
     8761 gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc ggggagcagg
     8821 aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg gcaaaagcag
     8881 ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa ggattccagc
     8941 aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat ggtgatttaa
     9001 cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat gatttgctac
     9061 tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt acagcaggaa
     9121 atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg atactaaaag
     9181 aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag tatttccttg
     9241 atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat gaacagatgt
     9301 ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa aaatcaggat
     9361 aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc atttgaaaca
     9421 acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc aaaaagatat
     9481 acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt gatccgacca
     9541 ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt atcaaaagat
     9601 attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt caatattttc
     9661 aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga tgggatgatt
     9721 agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca actagttttg
     9781 aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt tgtctttttt
     9841 atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt gtttctaagt
     9901 aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt gaaaaaagaa
     9961 tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag agggatgttt
    10021 atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt gttcttttta
    10081 tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat catctggtat
    10141 ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact aggaatgatt
    10201 cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga taaaaaagct
    10261 cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc agtagtcgga
    10321 acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat gcgttcggtc
    10381 gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt tccatcttct
    10441 cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt catcattgcg
    10501 ccagctctag gtgcggcttt gttttcagtc gttcctgtca attaccttat tttattagat
    10561 gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc aaaagtttct
    10621 cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg gctgcagcaa
    10681 ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt gatgctgttg
    10741 ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg tggttccgtt
    10801 ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg cggtgcactg
    10861 atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc ctacatcatt
    10921 atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg gttcttgtac
    10981 tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac actgctgatg
    11041 gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg gaacttcaac
    11101 tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt agctgatcgt
    11161 ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg cggaatcgtt
    11221 ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa actggtaaaa
    11281 gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa gtcttgttac
    11341 aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg aaaaatatga
    11401 gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga cctatcgtac
    11461 ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt gccaaatggt
    11521 agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac atcgttctcg
    11581 ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc gttaggcccg
    11641 agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac gatggcacct
    11701 acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt tttttctaaa
    11761 taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt tccttgcagt
    11821 tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg catagcaagg
    11881 tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct agtgatcaac
    11941 tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt ttttcctaat
    12001 aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct tttgcctccc
    12061 ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct aaaaagaaaa
    12121 ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa atagtttttc
    12181 acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga gtatgccaat
    12241 tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat gtttgtgtac
    12301 tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg aaattttcag
    12361 aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt agatgtttca
    12421 gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg cgttgtcgta
    12481 gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga acaaggatac
    12541 gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg tgtatgcacg
    12601 gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat tccttattac
    12661 tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct agccgaatac
    12721 cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa attcaaagca
    12781 ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca ttatgcgcaa
    12841 gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa taataaagat
    12901 caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat gttccctcta
    12961 ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct tgcgactgct
    13021 aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa acaatttttg
    13081 agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga agtcaaagga
    13141 caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg gatcggtggc
    13201 ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac aaacacatta
    13261 tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga tgcaagtgaa
    13321 atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc gaaattccgt
    13381 tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg tgcagaagtc
    13441 gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt ttatgatgga
    13501 atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa agttttacaa
    13561 tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga aacacctttt
    13621 cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat tttatacttg
    13681 aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc tttttgttaa
    13741 gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca attttataag
    13801 tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga ctggttgctc
    13861 tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat ctggctagtt
    13921 ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt attttagctt
    13981 tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt ggtattttcg
    14041 gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg ggttactggt
    14101 tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc atgggagaat
    14161 ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca agtattttta
    14221 tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc atcaatacaa
    14281 tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta tttatcgcat
    14341 ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat ctagtgaatg
    14401 gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg gttatgatgt
    14461 gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag aaacgttcag
    14521 aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat atccttgctt
    14581 cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca caacctgcta
    14641 tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc aatatcggct
    14701 tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca gaaacaacga
    14761 tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat aaaaaaaatg
    14821 ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta ttgactttct
    14881 tagttactga ttatgcctat caatttgcct attctttatg tacagcggca attttgattt
    14941 gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga atcaaaaaac
    15001 aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt ttagcaggat
    15061 tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc tactggatcg
    15121 cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt gggctgattt
    15181 gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag atataagggg
    15241 gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa atgggaaaag
    15301 aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga cgaactcgtt
    15361 ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga accattggac
    15421 tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct ttatcaaacc
    15481 gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct tttagctcta
    15541 tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta tcagcaatta
    15601 tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa ggaagaagaa
    15661 gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac aaaaatcatc
    15721 tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac tttagaaaaa
    15781 atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt atatgcgcct
    15841 ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg tatagcaacc
    15901 aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac agctactgga
    15961 cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg cggcggagtc
    16021 ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt ggcgcgaaac
    16081 aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa tgaaccgaaa
    16141 gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc catcgaagaa
    16201 gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt agtaccagga
    16261 agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa agaaaccttg
    16321 gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac aaaaacactg
    16381 caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta ttgtctatga
    16441 gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc agtttcttgc
    16501 tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac aagaagagaa
    16561 gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa cactggagcc gatcataggc
    16621 cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg tccagcagct
    16681 tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca agcatcagag
    16741 ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa tgccattggg
    16801 atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact cccaatcttg
    16861 tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg tatcgaagca
    16921 atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg aatgtacaga
    16981 ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt gacgaacata
    17041 ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga aaccatgttt
    17101 tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa gaagacgaaa
    17161 aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga agcagagatc
    17221 ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa ggttccaaga
    17281 acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc attactgata
    17341 taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac actagtcacg
    17401 caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag catcaatttt
    17461 cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt caccaagaaa
    17521 gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata attacctaac
    17581 gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc agccataaat
    17641 gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct aaataatttt
    17701 tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc tacagctctt
    17761 tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct ttctttttcg
    17821 tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa tgaaaaaaag
    17881 aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag agatggatgt
    17941 tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc cctcgctgta
    18001 taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac ttctgcagga
    18061 acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag cactgcagat
    18121 ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg agttactgat
    18181 cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa cgaatcagat
    18241 cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc atcgaagcag
    18301 ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat actttacaaa
    18361 agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact ttatcgctac
    18421 tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta aatgaccctt
    18481 ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc tagaaaagct
    18541 gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa tgagccttgg
    18601 tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga gtctttcgaa
    18661 gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta tgaatatatc
    18721 aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg ctggtgtgaa
    18781 gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa tcatcagccc
    18841 atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg aaaaaccaag
    18901 cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa attattatga
    18961 aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga aaatgccgga
    19021 agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga tccctaaaaa
    19081 actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca agcagctggc
    19141 tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag aaggggaact
    19201 tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc ggttatggat
    19261 ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac ctgcgaaaga
    19321 atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc tggtaggatt
    19381 gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag gccgtgtgca
    19441 gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt tccgtccaca
    19501 aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc ttcaaaaaaa
    19561 tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga tcagtacaag
    19621 caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc cattgcctta
    19681 tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg caaagaaaac
    19741 attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc cgcgtacaga
    19801 cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc aagcagtcag
    19861 cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc aacagacaaa
    19921 agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg aaaacagagc
    19981 acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg tctctcgttt
    20041 tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg ttgaatttgg
    20101 taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga aaggcgaaac
    20161 tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc ccgatttcgc
    20221 catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct tgcttggtca
    20281 aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg agaaactaat
    20341 caaatctgaa ctgatggaac gtacccctca aggactgcaa gtatctccta aaggtaagca
    20401 attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa gctgggaaaa
    20461 agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga atggaatcga
    20521 aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat atcaagattt
    20581 ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga aaaacacacg
    20641 tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa gaaaagatcc
    20701 aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca tcgaaggtaa
    20761 aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc ttgataaaaa
    20821 agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa aatatgcaca
    20881 agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa tgaagaagta
    20941 ataagatatg aaagaggttg tgatagaagt ggtcagcttc aagaaataag aaggaattta
    21001 cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa gctacttctg
    21061 gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt cctttttcat
    21121 ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat tcatttggct
    21181 tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct aagaagattt
    21241 tcccaatata tttcccgatg attccaagac ataacaattg gattccacca atgaacagga
    21301 agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat gcccgcaaaa
    21361 taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata aatgaagcaa
    21421 tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg aataatttcc
    21481 agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa atatattctg
    21541 ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct gttaattcta
    21601 ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca tcgaccattt
    21661 ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa ctgcgtatcg
    21721 caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca gaagtagtga
    21781 tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca tccatcacgg
    21841 tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct ttcccaaaat
    21901 ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc ttgattgctg
    21961 ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca tgggcacctt
    22021 tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta aaacatggga
    22081 caacaattga tactttcatc gtttctatcc tttcaatcgt atgtttatat atggtttaca
    22141 ttcaaaattt aataaacctt aaataacacc tttaaaattt gattttacgt aaaagaacag
    22201 gtattctttt tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt atcaaacttt
    22261 taagaacaat ttttattagt tggcaagtag ttttggatag agaaaaagga ggaagttcca
    22321 gtctattgac taggagcaaa caaaatgaaa atacacacac ctatcggctt tttatggtta
    22381 gatgcagatg aatccggatt gactgctgta tcttttcagc caatttcaga aagcgaatca
    22441 gcgaatcagc gaattttaac gcgcacacaa gaacagtttg aagaatattt tgcgggaaaa
    22501 agaaaagaat ttgatgttcc tttatctata aagagaggta cgccttttca gcaaaatgtc
    22561 tggaaagctt tgtcgaccat tccttatgga gaaatccgaa cgtataaaga agtagccgtt
    22621 gctgttgata gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac caatccattg
    22681 ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac aggatatatg
    22741 ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt tgttgttaga aggatatctg
    22801 caaaattaaa aacagttgtg attcggacat tgttccatgc gagaatcaca actgtttttt
    22861 catgttcatt ctaaactttg atcagccaaa taaatagtaa agccatcact gtttggagaa
    22921 gcccgacagc taatccataa gataagaagc gtgtaccttc tttcatgaat tttgcaaagt
    22981 ctaaacgcag accaattgcc gctaaagcag tgatctcaaa ccagctgctg ataaaatgtg
    23041 ccgattggtc aataactaca ggtaaagaaa acaagctgtt gaccaaacaa aagatcacaa
    23101 accctaatac ataccaagga agcggtaatt ttcgtttttt ctgcgtatca gttggcgttt
    23161 caaacagagc tggttgtttc ttgtgcatga atttttcaaa cagatagaca acaacaacga
    23221 gcatcataat acgtagaatc ttaaatagca tggcgaattc gacaacagaa ttgtcaagta
    23281 agcttgcccc agcgactact tgaccaacag attggagtgt accacccaaa agtgcacttt
    23341 tttcaatgag atttccacca aacaactgta atccaagaaa agggagagtc aacatcatca
    23401 cagtacccag tagattgact agtgtgatga tctgtccttt ttcttcctct ttcgcatgga
    23461 tggaaggggc gattgaagca atagcagaag aaccacaaac agcgtttcct ccagccatca
    23521 tcaaagaaga agtatcgctg aattgcagtt ttttaccaat caagtaagca ccgatgatag
    23581 tgactgacat ttgcagtaac acaaaaacaa ttccttttac accaatttga gcaatcgttt
    23641 gagctgtgac agttgcacct agtaaaaaaa cagagaattc taatagtttg ctttcagcaa
    23701 attttgtgcc gattgctaga acaggctgtt taaaaaaagt atttcctaat aaaataccca
    23761 ataaaatagc aatagtcgct ccacctaact gtggcagata aatagcaaga agcctgctga
    23821 aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat ttcgcggacc gctgaatcct
    23881 ttacagattt tattggatgt gacaatgatt tcatttgatc ggttcctttc gtcaataaat
    23941 actaaaagca tactcgaaca tagtagcact aaaccagtat tttgacaaaa gaacaagagg
    24001 tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa cggtgttcca gaagtagctt
    24061 ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct
    24121 tatttcttga agctgaccac ttcttttacg acctcttggt aaacggtgtt caaaagcttg
    24181 catctgcggt aataagccca accagtacaa aaacatgaac agctgttttt gtttggccgt
    24241 tcttattact tgatgcagaa cagctttttc acaacctatt gaatcgatta ttaagcgatt
    24301 tttttagaaa caaattttcc atgtttgcta tgttctttaa ttaagaaacg tacgtaatga
    24361 gcaagaacgt agaagacaac tacacttgca aacagacatt ccgcaacttt taagaaatcc
    24421 aatacgctac tatagatacc taaactgttg aagtaatgaa cagtagtaaa tatagctgtt
    24481 gcagagataa caagcgggaa tgtaaaggct gcatagcttg gataaaaacg aagcttgaac
    24541 aaacgtggaa gcatagacag cacaacgaaa tacaatactt gagataaagc gaacaatgct
    24601 acgacaagat aaaaattagg ttcagcaaag tttttaagat aacctgttag gcaaagagaa
    24661 ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat gagccatatt tcttacgaaa
    24721 aagacacgtt tgatgatgat cggcaataag aacgcatagc agatcaatga aatccaaaag
    24781 aaaatttttc caataagtgg gaagaagttc ccggcagtta cagtgatgac cccaaatcca
    24841 acgtaaatga taaaccagct tggataaatt gcttcaattg aaagatcagc tttgataaca
    24901 tggtgataat tgaaataaaa gacaaggatc acttggaaga taacagcgca cagccaaatg
    24961 aatttgacaa ttggtgcaat actttcatat gaaacaaaat aagtacaaat aaccatgagt
    25021 gacatcgtaa aagttggtga aacggaggcg atgattggat catgcaacgt ttgtttcgtg
    25081 tgttcgaata agataaacaa tttaacaaaa atgaatatca taagaatcat agagataccg
    25141 ccagttagat tgcctaaagc aacatggtga tagtctttaa acagatttcc aagggaagct
    25201 aacccaagga tcaacccgca gatcgggatc ggaataattt tcaaaaagtt cttcatatat
    25261 aatattctcc tttttattta atagatttta gtaccaagat gaacgaatac attgtaaaat
    25321 gaataggaat gtatgattac gagcaagcag acagctgatt gtcttcatct aagttactag
    25381 ttggatcagc aactgttttg ttgtacattt ctattataga tggttttttg tatatttaaa
    25441 tcaaatagtt tttatagtaa ttatcataaa aagttatgga agtggtgcca tgtttaagtt
    25501 attaacgact tttcgagcag tatatgaaac aagaaatttt tccagagcag cagaagtact
    25561 gtttttatct caaccagcag tctcgaatca aatcaaacag ttggagcggg aattgaatat
    25621 cgatcttttt caacgtaacg gaagaaaaga gatgcttcca actaagcaag ccgacttatt
    25681 atatgaacgg acactggttt tgttagaaga atgggaagaa actcagcaaa aattgctcaa
    25741 tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc cacacctttg ctgtctatct
    25801 tttgcctcag ttgatgaaac atctgatcaa aaggtttcct aaagtgcatt ttgagatcga
    25861 aatcacgaat tctcatgaat cattagaaca agtagcgaaa catgaatccg attttggttt
    25921 tatcgaaaag ccattagaga cttccggcgt acaaagatac tctttgatgg aagaccagct
    25981 agtcatcgct ggggacccaa aaagcaaatt gtggcttgta agagaagcaa actctggtgt
    26041 ttttcattac acgcagcgtt attttgaaga gatgaatatc caagataaga agatgatcgt
    26101 gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga atcggtcgct cgattttgtc
    26161 taagcgctct gttccagaaa atgtgccaat gactccgttg aatttccatc gatattttta
    26221 tttcatccaa cgaaccaatc tggtttctga tcagctgcta gagattgccg aagagatcct
    26281 tcgatacaat gaaagcacag aaacttctca cagcgaggag aatctcagct ggtagataat
    26341 tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa gcagtaggct tttgtactgc
    26401 atttgctcaa gcaactgcac aaacggaaag ttaatttttc ccaaatgtct atggaaagac
    26461 tttccattat ctaactggac cgctataata atcagcagta tggtttttaa gaaaagggat
    26521 gaaacatgga aaaaaaatta aaagcttcga acgataatcg tattgattat ggcgtgattt
    26581 taccagtctt tctgttatgt ttgattggtc tgctctcatt atatgtagca cttagccatg
    26641 atccgaacaa tccagcagtc ttgaaaggcg tagggatgca agttgtctgg tatttagttg
    26701 gagcggcagc aatcgttgtc atcatgcatt tgaattccaa atggatctgg aaactgactc
    26761 cttaccttta tggtttgggg ttagtggtaa tgggactctt gctcaaattc tatgatcaga
    26821 atttagctgc ttcaacaggg tctaaaaact ggtttcgatt cggtgcgttt actttccagc
    26881 cagcagagtt gatgaagatt gcgtacatct tgatgatggc actgatcgta actaaacata
    26941 atacgcaagt aaaagaacgg acgatgaaat cggacttttg gctgattggg aagctcttga
    27001 tcgtgacaat accggtttta gcattgatca tggcccaaga tgactttgga acgatgcttg
    27061 tctttcttgc gatctttggc gggattttcc tgatgtctgg aatctcttgg cgcatcatcg
    27121 cacctgtagt aatcttggca gtagtcgttg gagcaggaac gatcttcttg gttacgactg
    27181 aaggcggaag agacttgctt tataaagttg gattcaaatc ttatcagttt gcacgtattg
    27241 attcatggct ggaccctttc catgatacgt caggaatgag ttatcagccg gcccaaggac
    27301 ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt caacgtaagc aatatctatg
    27361 tgcctgttcg tgagtccgat atgattttta ccgttatcgg tgaaaacttc ggctttatcg
    27421 gtggtgcatt cgtcattttc ttgtatttta ttttaatcta tcgtatgatt cgggtgtgtt
    27481 tcgatacgaa taatgaattt tacgcctata tcgcaagcgg tttgatcatg atgctgctgt
    27541 tccatgtctt tgagaatatt ggagcaaata tcggattact tcctttaaca gggatcccat
    27601 tgcctttcat tagtcaggga ggatcctcga ttttagggaa catgatcggt atcgggttga
    27661 tattatcgat gagataccaa aatgaagcac ctgcaagatc ttcaaggagg taagaaaatt
    27721 gtacaccttt tactggtacc cgaaatgttc aacatgcaaa aaagccaaag cgtggttaga
    27781 ccagcatggc gttagttatc aaaccgttga tatgatcaaa gaagtaccga aggcctcaac
    27841 gattgagact tggctagaaa cgagtcaagt accaatccgt cgcctgttca acacaagcgg
    27901 gatgaaatat cgggaacttg gactgaaaga tcaagtaggc gagtttacgg cttcaaaagc
    27961 gagccaagtc ttatcgacag atggtatgct gatcaagcga ccactgatct tacgagagga
    28021 ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa ggagaactga tataaaatgg
    28081 caggcaagca tttgaagaaa aaggacaacc tttggatctt atttaatgga aaagaatact
    28141 gtgttggttt gacaaatgaa gcgcaagaag aacttggaga catcacgttt gccaatctgc
    28201 caaaagcagg acaatcgcta aaaaaagatg aacctctgat cgaagttgaa gcggaaaaag
    28261 cggtcaatga atttgccagc ccgctaacgg gtacagtttc ttctgttaat gataaaatcg
    28321 gtgaagatat caatatcttg aatgattcag atgaaatgaa tgcgtggatc ttaagtttca
    28381 aagatgttga tccaaaagaa tttgacgaat tataaaatca gaaaatcact tgaaaaaagt
    28441 tgacactgat tcttccagtt gctataatca taagcaatcc tatatgaaac gagaaagctt
    28501 tgaaaagaag agtacatgtc acggacgttt gaagagagcc ctgtttgctg aaaatgggta
    28561 acgaaaatga gatggaagat ggtcttagag cagagtaagc gagcgtaagc aaacttacta
    28621 cgggagtgcc cgttacagcg cgacagtata cggaaagaag ctaagtccaa gtactggaag
    28681 aggctgttat tgcgaagtaa cggtaaatca aggtggtaac acggaagttc aagctttcgt
    28741 ccttttgctt aaggcaagag ggcgaaggct tttttgtttg aaaaattata agagaaagat
    28801 gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa agacattcaa tgggaaaaac
    28861 ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg aaaaagggga tatttatgga
    28921 atcgtcggct attctggcgc aggaaaaagt acattagtcc gtctcctgaa tggacttgag
    28981 cttcccacga gcggcgaagt gatcgtaaac gatcaagata ttacaaaatt aaaaaacaaa
    29041 gaattacgta cattccgaaa gaaaatcggt atgatcttcc agcatttcaa cctgttatgg
    29101 tcaagaactg tacttgaaaa tatccagctg ccgctggaat tagctggcgt accaaagagc
    29161 aaacgtaaag aacgggcaga agaactgttg cgtttagtcg ggttagaagg acgcggacaa
    29221 gcatatcctt cacaactttc cggcggacag aaacaacgcg tcggtatcgc acgagcattg
    29281 gcaaatgatc ctgagatcct gctttgtgat gaggctacta gcgcattgga cccgcaaaca
    29341 acagaagaag tattagactt attgttagcg atcaataaga aattgaattt gaccatcgtc
    29401 ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca acaaagtcgc tgtgatggag
    29461 ttggggaaag tcgtagaaga aggagacgta ttgactgtct tcagacaacc gaaaaaagaa
    29521 gtaaccaaac gctttgtaca acaagacatc gaaccagaag ctgattcgac agaactatta
    29581 gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa ctttgacctt tagcgaaaga
    29641 aatgccaacg agccagtcat ttcccaagcg attcgacgct ataacatcga tgtcaacgtt
    29701 gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg gttcattgac tgtcttgatg
    29761 actggtgaaa ccaaagaatt agatcaagca gaagtgttca tcaaagaaca aggagtagga
    29821 attgaggtga tccatcgtgg ataaaagctt ttcagaaaca tatctagatt ttaaacaaat
    29881 caacccggaa acgatgaatc aggctatcct tgatacacta tatatgacat taatctcaat
    29941 ggtcttagtg acgatcatcg gattagtttt gggattgatc ctttactcta ttggacgcaa
    30001 aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg atcatcagta atattttccg
    30061 gtcaacgcca tttatgatcc tgatggtttt gatcattcca tttactaaag caatcgtagg
    30121 cactatgcta ggtgcgcaag cagccattcc agctcttgtt ttatccgcag caccatttta
    30181 tgcccgtcta gtagagattg cttttcgtga agtcagccct ggtgtactgg aagcagcaga
    30241 tgcaatggga gcaagttact gggaaatcat ctggaaggtc ttgatacctg aaagtattcc
    30301 cgcattgatt tctggattaa cagttacaac gatctcgatg atcggattta ctgcgatggc
    30361 cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg caggaaggct accagcgtgg
    30421 taatctaaca gtgacattcg ttgcgacttt aattatttta ctaatcgtat ttatcgttca
    30481 aggaatcggt gatttcttaa ctaagaaaac agacaaaaga taataggata tagggggatg
    30541 taggatatga aaaagaaaat ttttggattt gcagcagcgc ttttgctgac tgtaggatta
    30601 gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa ataaagcaga cgacacgaca
    30661 ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt tggaacatgt gaaaccatta
    30721 ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg atgactatgt attaccgaac
    30781 caatctttgg aagaaggcga cattgatgcc aattacttcc agcatattcc ttatttgaac
    30841 aaagaaatca aagaaaaagg atatgacttt gtcaatgccg gtgctgtcca catcgaacca
    30901 atgggcttgt actctaaaaa agtcaaagat gtttccgaat taaaagatgg tgcaacaatc
    30961 attacttcaa attctgaatc agactgggga cgcatcatca caattctaca agatgctggc
    31021 ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa cattcgatga tattgaagaa
    31081 aaccctaaga acttgaagtt caaccatacg atcgacccat cacttttagc ttctacgtac
    31141 caaaatgatg aaggtgacct agttgcaatc aacgcaaact tcgcttacgg cgcagggttg
    31201 aatccattag aagatgccgt attgcttgaa aaggacaact ctccttacgt gaatattatt
    31261 gcgactcgta aaggtgatga agacagtgac aaaatcaaaa aactgattga agtgcttcac
    31321 cgcaaagaag tccaagaatg gatcacagac aaatggggcg gctctgttaa accagtaaag
    31381 gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa taagtctgta atggcttatt
    31441 tctcatcttt tttataccta ttctaattta gaaatcaaaa ttgtttaata ataaataaaa
    31501 gatatttgat tttagtaagg aaggtcattg cgcttcattc tcaattagtc taaaatgagt
    31561 gagaacgaaa gagaatttgg gggtataatg catgtctgtc ttagaaataa aaaatctaca
    31621 tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac ctaacaatga aaaccggaga
    31681 aatccatgcc atcatgggac ctaacggcac aggaaaatct accttatctg ccgctatcat
    31741 gggcaatcca aactatgaag taacagaagg cgaaattttg tttgacggga aaaatgtttt
    31801 agacttggaa gtagatgaac gtgcgcgact tggcttattt ttagctatgc aatatccaag
    31861 tgagatacca ggtatcacga atgcggaatt catgcgtgca gcgatcaatg cgaaacgtga
    31921 tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag ttagatgaaa aaatggcttt
    31981 actaaatatg ccagaagaaa tggctgaacg ttatctgaac gaaggttttt ctggcggaga
    32041 aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa cctacatttg caattttaga
    32101 cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc gttgctaaag gagtaaatga
    32161 aatgcgcgga gacaatttcg gtgcattgat catcacccac tatcaacgat tattgaacta
    32221 tatcacacct gatgtcgttc acatcatgat ggaaggccga gtagtcatga cagggaatgc
    32281 tgacctagct aaacgtctgg aagcagaagg ctacgctggt atcagtaaag aattaggaat
    32341 cgactataaa gaagaagaag cgtaaggggg agcaagaatg aaagaaaaaa actggctcga
    32401 ttatcttgac gcggtaaatg atttttcttt aagtaaaggc gaacctgatt ggatgagaac
    32461 cttccgtcaa gacgcactag cgaaagcaga tgaactgcca ttgccgcata tcgatcgggt
    32521 gaaattccat cgctggtcgt tatttgatgt gaaagaaaca caaaccatca gtgaaacagg
    32581 cacgattccg gcatttgatg caatgaaaga caatcctgtg cttgtccaac aaggttcatg
    32641 gacgattttt gaacaactgc ctgtggaatt agcagaaaaa ggagtcattt ttaccgactt
    32701 gttcactgca atgatcgaat atccagaact agtccaagaa tattatatga aaaaagcagt
    32761 aaacatgaac gaagaccaat tgactgcact ccatgtagct tttatgaaca gcggtatttt
    32821 cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc gagtccttat tcattcaaga
    32881 tggcgcatcc gatgagcact tcttcaagca tgtgttgatc gtggcagatg aacatagtga
    32941 attttcttat ctggaacgtt tccagacaac caaagaacaa gtagcaaaat catcaggtaa
    33001 tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc aaatattcag ccgtagatca
    33061 attaggagaa aatattactt cttatatgaa tcgccgtggg cacattttac gagatgcttc
    33121 tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg atcgctgact ttgactcaga
    33181 tttagcagga gaaggtgcac acgcagaagt caaaatcgtg gcaatcagct cgggcagaca
    33241 aatccaagga atcgatacac gagtaaccaa taaagcccct catacgatcg ggcatatttt
    33301 gcaacacgga gtgatccgag aaaaaggaac attgaccttt aatggtatcg gccacatctt
    33361 gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt gtcttgatgc tttctgataa
    33421 agcacgtggt gatgcgaatc ctattctttt gatcgatgaa aacgaagtaa ctgctggaca
    33481 tgctgccagt gtaggacgag ttgatccaga agaaatgtac tatctgatga gtagaggatt
    33541 gcataaagaa gaagcagagc gcttagtgat tcgaggattc ctcggttctg tcttaacagc
    33601 aatccctgta gaacaagttc ggaaagaatt agtcgaagta atcgaaggga agttgaatgg
    33661 atgatcgatg ctaaaaagct acgcaatgat tttcctatct tatttcaaga cgtcaatgat
    33721 gaacctttag tttatctgga taatgcagcg acgacccaaa agccaaaaca agtactccag
    33781 gcattagcgt actattacga acacgataac gccaatgttc atcgcggtgt ccatacgtta
    33841 gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa aagtacgggc gttcatccat
    33901 gcaaaagaaa cagcagaagt gctgtttact cgtggtacga cgacaagctt gaattgggta
    33961 gccagaacct tcggcgaccg ctatatcaaa gcaggagacg agatagtgct ctcttacatg
    34021 gaacatcatt ctaatgttat cccgtggcag cagttggcaa aacgaacagg tgctgtcttg
    34081 aaatatatcg aactaacaga agaaggcttc ctggatatgg aggatgcagc gaaaaagatt
    34141 acggacaaaa ccaaaatcgt ctccattgct catgtatcca atgtcttagg tgtcttcaat
    34201 cctgttcaag aattagctga acttgtccat gaaaacggcg gggtcatcgt agtagatggt
    34261 gctcaatctg ccccgcacat ggccattgat gtccaagcac tagactgtga tttctttgct
    34321 ttcagcggac acaaaatgtg cggaccaaca ggtattggtg ttttatatgg taaacgaaaa
    34381 tggttagaag aaatggaacc tgttgaattt ggtggcgaaa tgatcgattt tgtcgaatta
    34441 tacgacagta catggaaaga actaccttgg aaattcgaag cagggacacc aaatatcgcc
    34501 ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa aactcggtat ggacaatatc
    34561 catcgatatg aagaagaact agccgcttat gtgttgccaa aattacaagc aattgatgga
    34621 ttgactgttt atggtccaca agacccgaat tttcggacag gagtcgttgc attcaatttg
    34681 gatgggttac atccccatga tgtcgcgaca gctttagaca tggaaggggt agccgtcaga
    34741 gcaggtcatc attgtgcgca accgctattg aagtaccttg aagtatcagc gactgctcgc
    34801 gcgagctttt atttctacaa cacaacgcaa gacgccgacc gtttagttga agcaatcatg
    34861 gcaacaaagg agtttttcca acatgggact atctaaatta gacaatttat accgtcaagt
    34921 gatcttggat cattcttccc atccgcatca tcgtggaagc ttgactgact cgacaagcaa
    34981 gatcgaattg aacaacccca catgtggaga tgtgatcgaa ttgcaagtcg atgtaaaaga
    35041 cggaatcatc aaaaatatcg cctttgacgg cagtggctgc tccatcagta cggcaagtgc
    35101 ctcgatgatg acagacgccg tcatcggaaa atcaatagaa gaagcagaac atttaactgt
    35161 agactttcta cagcttgtcc aaggaaaaga agtcaaagat cccgaccaat taggcgatgc
    35221 tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc aaatgtgcga cacttgcctg
    35281 gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca gctttccatc attaccacaa
    35341 ggaagaggag tgagaacatg ggcgtaccag aattagaaga atataaattc gggtttcacg
    35401 atgatgtcga accggttttc agtacaggag aaggcttgac agaagaagtg atccgtgaga
    35461 tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt ccgtctgaaa tctctagaaa
    35521 catttaacaa aatgccaatg caaacatggg gccccgacct ttcagacatc gatttcgatg
    35581 ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg tgactgggaa gacgtaccag
    35641 aaaaaatcaa agatacattt gaaaagattg gtataccaga agctgaacga gcttatctag
    35701 ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca taatatgaaa gatgaattcg
    35761 aaaagttagg aatcgtcttt actgacactg actctgcttt gaaagaatac cctgaattat
    35821 tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa caaattagca gcgctgaatt
    35881 cagccgtatg gtcaggtggg acctttattt acgtgccaaa aggcgtcaaa gtcgatgttc
    35941 cattacaaac gtacttccgg atcaatgcgg aaaacacggg gcaattcgaa cgtacgctga
    36001 tcattgttga cgaaggagca agcatccact atgtcgaagg ttgtacggca cctacttatt
    36061 caagtaacag cctgcatgcc gccatcgtag aaatctttac aagaaaagat gcgtattgcc
    36121 gttacacaac gatccaaaac tggtcagaca atgtgtacaa cttagtaacc aaacgagcaa
    36181 aagcatacga aggcgcaacc gttgaatgga tcgacggaaa cctaggtgca aaaacaacga
    36241 tgaaataccc aagtgtttac ttagatggaa aaggtgcacg aggcaccatg ctctccatcg
    36301 cctttgcagg agcaaaccaa atccaagata cgggtgcaaa aatgatccac aatgcaccaa
    36361 atacatctag ctctatcgtc tctaaatcaa tttcaaaaga cggcggagaa gtaaactatc
    36421 gaggacaagt aacctttgcc aaaaacagtg cgggttcgat ctctcatatc gaatgtgaca
    36481 cgatcatcat ggacgaatgg tcgaaatccg atacgatccc attcaatgag atccacaaca
    36541 gccaagtctc tttagaacac gaagccaaag tctcaaaaat ctcagaagaa caactctact
    36601 atctgatgag ccgaggcctg accgaatctg aagcaactga aatgatcgtc atgggctttg
    36661 ttgaaccatt caccaaagaa cttccaatgg aatatgcagt cgaactcaat cgattgatca
    36721 gttatgagat ggaaggcagt gttgggtaaa cctgttatag gagctagaac ccttgatata
//
LOCUS       DASJUF010000014        36470 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJUF010000014 DASJUF010000000
VERSION     DASJUF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.014"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            271..591
                     /locus_tag="U4B62_000640"
     CDS             271..591
                     /locus_tag="U4B62_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN2140822.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            745..1650
                     /locus_tag="U4B62_000641"
     CDS             745..1650
                     /locus_tag="U4B62_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2140823.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1647..2420
                     /locus_tag="U4B62_000642"
     CDS             1647..2420
                     /locus_tag="U4B62_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2140824.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2601..4025)
                     /locus_tag="U4B62_000643"
     CDS             complement(2601..4025)
                     /locus_tag="U4B62_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2140825.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4236..4682
                     /gene="nrdI"
                     /locus_tag="U4B62_000644"
     CDS             4236..4682
                     /gene="nrdI"
                     /locus_tag="U4B62_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN2140826.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4747..6729)
                     /locus_tag="U4B62_000645"
     CDS             complement(4747..6729)
                     /locus_tag="U4B62_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2140827.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="U4B62_000646"
     CDS             complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="U4B62_000646"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN2140828.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7642..8703)
                     /locus_tag="U4B62_000647"
     CDS             complement(7642..8703)
                     /locus_tag="U4B62_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2140829.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8914..9480)
                     /locus_tag="U4B62_000648"
     CDS             complement(8914..9480)
                     /locus_tag="U4B62_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2140830.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9508..11010)
                     /gene="eat(A)"
                     /locus_tag="U4B62_000649"
     CDS             complement(9508..11010)
                     /gene="eat(A)"
                     /locus_tag="U4B62_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2140831.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11268..12389)
                     /locus_tag="U4B62_000650"
     CDS             complement(11268..12389)
                     /locus_tag="U4B62_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2140832.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12515..13456
                     /locus_tag="U4B62_000651"
     CDS             12515..13456
                     /locus_tag="U4B62_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2140833.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13606..14502)
                     /locus_tag="U4B62_000652"
     CDS             complement(13606..14502)
                     /locus_tag="U4B62_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN2140834.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14771..16111
                     /locus_tag="U4B62_000653"
     CDS             14771..16111
                     /locus_tag="U4B62_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2140835.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16329..16772
                     /locus_tag="U4B62_000654"
     CDS             16329..16772
                     /locus_tag="U4B62_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN2140836.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="U4B62_000655"
     CDS             complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="U4B62_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2140837.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19097..20320)
                     /locus_tag="U4B62_000656"
     CDS             complement(19097..20320)
                     /locus_tag="U4B62_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN2140838.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20351..21190)
                     /locus_tag="U4B62_000657"
     CDS             complement(20351..21190)
                     /locus_tag="U4B62_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2140839.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21207..21887)
                     /locus_tag="U4B62_000658"
     CDS             complement(21207..21887)
                     /locus_tag="U4B62_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2140840.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21889..22962)
                     /locus_tag="U4B62_000659"
     CDS             complement(21889..22962)
                     /locus_tag="U4B62_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2140841.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23018..23256)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23299..23532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23609..24280)
                     /locus_tag="U4B62_000660"
     CDS             complement(23609..24280)
                     /locus_tag="U4B62_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2140842.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24367..27045)
                     /gene="mgtA"
                     /locus_tag="U4B62_000661"
     CDS             complement(24367..27045)
                     /gene="mgtA"
                     /locus_tag="U4B62_000661"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN2140843.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27704..28141
                     /gene="ndk"
                     /locus_tag="U4B62_000662"
     CDS             27704..28141
                     /gene="ndk"
                     /locus_tag="U4B62_000662"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN2140844.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28163..29029)
                     /locus_tag="U4B62_000663"
     CDS             complement(28163..29029)
                     /locus_tag="U4B62_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2140845.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29189..31282)
                     /locus_tag="U4B62_000664"
     CDS             complement(29189..31282)
                     /locus_tag="U4B62_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2140846.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32031..32654)
                     /locus_tag="U4B62_000665"
     CDS             complement(32031..32654)
                     /locus_tag="U4B62_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140847.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32813..34210)
                     /locus_tag="U4B62_000666"
     CDS             complement(32813..34210)
                     /locus_tag="U4B62_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2140848.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34242..35213)
                     /locus_tag="U4B62_000667"
     CDS             complement(34242..35213)
                     /locus_tag="U4B62_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2140849.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35587..36012
                     /locus_tag="U4B62_000668"
     CDS             35587..36012
                     /locus_tag="U4B62_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140850.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        10553 a         7602 c         6390 g        11925 t
ORIGIN      
        1 tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag cattcatctt
       61 cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa gtatgagatt
      121 gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg ccaccgggta
      181 ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct ctttttgtgt
      241 atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat catagcagga
      301 attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc taaaatcaat
      361 tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc aaaggcaatc
      421 aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc agttggttcg
      481 gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg gttctttgtg
      541 gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta gacacttgga
      601 ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt ttatgaaaaa
      661 ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat gcttgtacta
      721 tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac
      781 tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg caaaatcgtt
      841 ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg
      901 atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc ctctagtcat
      961 aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc attcttaaca
     1021 ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat cgacactatc
     1081 gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta ctcgttggga
     1141 atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta
     1201 gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc
     1261 gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa
     1321 aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca atgtacaatg
     1381 gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa tgatctagct
     1441 gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa aatcaagctt
     1501 ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc acaaaatatc
     1561 catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt ggaaattctg
     1621 gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa ttatttaaat
     1681 tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg ctcataggaa
     1741 cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc
     1801 tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc acgatcattt
     1861 cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag
     1921 aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta taccttttag
     1981 ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc ttcacggaga
     2041 aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg ggtagttata
     2101 tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc aatagttccg
     2161 gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata tctggtatcc
     2221 ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt atgttgaatc
     2281 tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt tctactaatc
     2341 aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga tatctcgtat
     2401 ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag acgttcagcc
     2461 ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc tgtgacaaca
     2521 tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc acaacctctc
     2581 tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa aaccattgca
     2641 atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat atgttgtaca
     2701 ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa gaaaagaaca
     2761 agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg aaaggcactt
     2821 gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag tttcatttgg
     2881 tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt gtaagcaaag
     2941 ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca taacgtcgtc
     3001 aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc tttaggcaaa
     3061 agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca tgagagccaa
     3121 acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca tttaccaacc
     3181 atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag ctgattcatc
     3241 gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa gccgaatatc
     3301 cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc tacgacagag
     3361 gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa tacttgactg
     3421 gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa gttatcaatg
     3481 aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa tggcactaat
     3541 ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg attcactagc
     3601 catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt ttgcccacct
     3661 tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa agctacgttg
     3721 cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa
     3781 ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt attgatatta
     3841 ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact gaaaatagct
     3901 gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc tccaatcgct
     3961 gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg ttgctctgta
     4021 tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac aaaccttttt
     4081 tatttttact taattaataa aaactttaat agaaatataa ttctaccggc ttttttctca
     4141 ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga
     4201 tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt
     4261 caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag
     4321 aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga
     4381 aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga aatggtttag
     4441 ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca
     4501 atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt
     4561 gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat
     4621 tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat
     4681 aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct
     4741 atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc
     4801 caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt
     4861 gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg
     4921 ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc
     4981 catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg
     5041 gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac
     5101 aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga
     5161 aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg
     5221 tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc
     5281 taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc
     5341 caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc
     5401 tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc
     5461 gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta
     5521 aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt
     5581 taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc
     5641 accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac
     5701 atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc
     5761 tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat
     5821 gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc
     5881 aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag
     5941 ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg
     6001 tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc
     6061 tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg
     6121 tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt
     6181 catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt
     6241 tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg
     6301 cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga
     6361 tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga
     6421 tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa
     6481 atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg
     6541 ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag
     6601 atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt
     6661 gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag tcagttcgtt
     6721 ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta
     6781 attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa
     6841 cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt
     6901 gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca
     6961 tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga
     7021 taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg
     7081 catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa
     7141 tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg
     7201 tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta
     7261 gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt
     7321 cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc
     7381 attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac
     7441 gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt
     7501 ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag
     7561 aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct
     7621 ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc
     7681 agctttgatt tggttgatca atggtagctc ttctttgata actttagcta ctacattcac
     7741 tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata
     7801 cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat
     7861 aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg
     7921 ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg
     7981 gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag
     8041 acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc
     8101 aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct
     8161 caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg
     8221 attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg
     8281 ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg
     8341 cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg
     8401 attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag
     8461 atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc
     8521 tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac
     8581 atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg
     8641 gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa
     8701 catattatcc ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa
     8761 gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt
     8821 atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt
     8881 gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt
     8941 atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac
     9001 tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc
     9061 tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg
     9121 tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata
     9181 atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc
     9241 agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat
     9301 tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc
     9361 agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc
     9421 gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat
     9481 accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga
     9541 tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa
     9601 gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca
     9661 tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc
     9721 catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag
     9781 gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc
     9841 tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt
     9901 ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc
     9961 tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg
    10021 ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg
    10081 gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt
    10141 ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt
    10201 ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa
    10261 tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc
    10321 tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa
    10381 ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag
    10441 ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg
    10501 gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc
    10561 agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa
    10621 taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca
    10681 taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa
    10741 atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg
    10801 gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag
    10861 aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg
    10921 tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa
    10981 tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc
    11041 ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa agacccatac
    11101 atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta
    11161 cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt
    11221 gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg
    11281 atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg
    11341 tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga
    11401 aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt
    11461 cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga
    11521 tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca
    11581 gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct
    11641 ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa
    11701 tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct
    11761 attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg
    11821 gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa
    11881 tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc
    11941 aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata
    12001 atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa
    12061 gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca
    12121 gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc
    12181 tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag
    12241 attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta
    12301 cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta
    12361 aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac
    12421 actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta
    12481 aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg
    12541 agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat
    12601 cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa
    12661 cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa
    12721 cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg
    12781 ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg
    12841 tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat
    12901 gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga
    12961 actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca
    13021 agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc
    13081 ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact
    13141 aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat
    13201 cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac
    13261 ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa
    13321 ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata
    13381 tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat
    13441 ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct
    13501 ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt
    13561 gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac
    13621 agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt
    13681 tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt
    13741 atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg
    13801 acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata
    13861 catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat
    13921 cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc
    13981 attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg
    14041 cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt
    14101 ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag
    14161 cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    14221 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac
    14281 aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc
    14341 taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga
    14401 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag
    14461 tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc
    14521 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct
    14581 atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat
    14641 taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag
    14701 tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa
    14761 gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat
    14821 gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg
    14881 gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt
    14941 gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg
    15001 tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc
    15061 tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg
    15121 gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat
    15181 caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat
    15241 cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg
    15301 cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg
    15361 tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact
    15421 gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa
    15481 aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct
    15541 ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa
    15601 aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc
    15661 atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg
    15721 aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt
    15781 tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa
    15841 attatttggc tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat
    15901 cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag
    15961 cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat
    16021 tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa
    16081 gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg
    16141 tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt
    16201 ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa
    16261 ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt
    16321 tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta
    16381 tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac
    16441 gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt
    16501 ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt
    16561 ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga
    16621 aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag
    16681 atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca
    16741 aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg
    16801 atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa
    16861 taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    16921 ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
    16981 tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat
    17041 agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt
    17101 ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat
    17161 ggctttccac atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg
    17221 tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt
    17281 gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc
    17341 cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga
    17401 atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc
    17461 cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag
    17521 ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag
    17581 ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa
    17641 tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc
    17701 caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca
    17761 tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg
    17821 aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa
    17881 taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct
    17941 ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa
    18001 agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg
    18061 tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca
    18121 tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg
    18181 attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa
    18241 ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg
    18301 acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag
    18361 aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga
    18421 tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga
    18481 tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt
    18541 ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga
    18601 cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg
    18661 cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc
    18721 ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc
    18781 ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct
    18841 taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca
    18901 tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca
    18961 cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt
    19021 gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac
    19081 acaagattat tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt
    19141 gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat
    19201 tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag
    19261 ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga
    19321 ttgttggtat gacttggctg acatttccta catcagaaga accgagactt gttctttgtt
    19381 ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat
    19441 caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag
    19501 tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca tatactttat
    19561 ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga
    19621 cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt
    19681 gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc
    19741 ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat
    19801 cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta
    19861 catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc
    19921 ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt
    19981 gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat
    20041 gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat
    20101 tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa
    20161 acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta
    20221 cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga
    20281 ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta
    20341 gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg
    20401 ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg
    20461 ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt
    20521 catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta
    20581 caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat
    20641 ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt
    20701 gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc
    20761 cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat
    20821 ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta
    20881 aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca
    20941 aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt
    21001 attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat
    21061 cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc
    21121 cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa
    21181 tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg
    21241 ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct
    21301 gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact
    21361 gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg
    21421 ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc
    21481 atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga
    21541 cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc
    21601 gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg
    21661 atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt
    21721 aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca
    21781 atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac aaattcatcc
    21841 ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc
    21901 ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc
    21961 agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac
    22021 gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc
    22081 tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat
    22141 ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat
    22201 gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc
    22261 ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc
    22321 cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac
    22381 gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt
    22441 tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa
    22501 agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga
    22561 tgctttttct tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg
    22621 tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc
    22681 cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt
    22741 gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg
    22801 cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc
    22861 ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc
    22921 tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct
    22981 aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt
    23041 taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct
    23101 agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga
    23161 tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag
    23221 gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac
    23281 taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt
    23341 tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac
    23401 agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt
    23461 acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt
    23521 caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag
    23581 tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa
    23641 taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg
    23701 tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag
    23761 cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg
    23821 ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc
    23881 cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc
    23941 ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca
    24001 atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat
    24061 attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta
    24121 aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga
    24181 tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt
    24241 caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca
    24301 aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct
    24361 tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag
    24421 cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat
    24481 caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa
    24541 tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt
    24601 acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg
    24661 gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac
    24721 tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact
    24781 tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc
    24841 gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat
    24901 cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc
    24961 aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat
    25021 gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt
    25081 ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat
    25141 accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac
    25201 agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt
    25261 tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat
    25321 tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt
    25381 aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt
    25441 caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac
    25501 catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc
    25561 gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc
    25621 ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt
    25681 cagcatttct tcaatcgcac cttttgtgat catttgtgtt ttaccagctg gatcagcaac
    25741 cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt
    25801 gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt
    25861 tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc
    25921 atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt
    25981 tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat
    26041 cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat
    26101 tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag
    26161 ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa
    26221 tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt
    26281 ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat
    26341 tccttccgca cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt
    26401 ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc
    26461 tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg
    26521 tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat
    26581 ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt
    26641 ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac
    26701 catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata
    26761 ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta
    26821 tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc
    26881 gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa
    26941 tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc
    27001 tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt
    27061 ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat
    27121 agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg
    27181 ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt
    27241 atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa
    27301 aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc
    27361 agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct
    27421 accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt
    27481 tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc
    27541 ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact
    27601 ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca
    27661 cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt
    27721 tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga
    27781 agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa
    27841 agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac
    27901 ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa
    27961 gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc
    28021 gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga
    28081 gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg
    28141 atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct
    28201 ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt
    28261 agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca
    28321 tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt
    28381 cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca
    28441 ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt
    28501 ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag
    28561 ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga
    28621 ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca
    28681 tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat
    28741 tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt
    28801 ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg
    28861 tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat
    28921 ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg
    28981 tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc
    29041 tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg
    29101 aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg
    29161 tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg
    29221 ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg
    29281 tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt
    29341 catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt
    29401 cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga
    29461 tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag
    29521 cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta
    29581 cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga
    29641 ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga
    29701 ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt
    29761 tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat
    29821 ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac
    29881 cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc
    29941 gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt
    30001 catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt
    30061 gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac
    30121 ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt
    30181 ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg
    30241 tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa
    30301 gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag
    30361 catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt
    30421 gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa
    30481 tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc
    30541 caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa
    30601 tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat
    30661 aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct
    30721 gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg
    30781 aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt
    30841 tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga
    30901 catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa
    30961 agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca
    31021 agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg
    31081 gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc
    31141 acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa
    31201 tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc
    31261 acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt
    31321 gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc
    31381 taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact
    31441 aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt
    31501 ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct
    31561 tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc
    31621 ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg
    31681 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgaccc
    31741 tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa
    31801 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta ttttttgaaa
    31861 ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca
    31921 atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact attttgccgt
    31981 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt tcattgtcca
    32041 taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg attttcttca
    32101 cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt cgactcgttt
    32161 ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca ttccgctaaa
    32221 cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac ttttccattt
    32281 ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa atgcttgtct
    32341 ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt aataaattga
    32401 ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta attggcagtt
    32461 attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc tactgattct
    32521 ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa
    32581 aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt
    32641 ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta ttatatacaa
    32701 aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc
    32761 acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt tcttaagcct
    32821 caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg attgttcttt
    32881 ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat gcattggtcc
    32941 acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca ccttcttgaa
    33001 tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa tcatcgtgga
    33061 tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg ttttcagaaa
    33121 taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca tagattcctt
    33181 gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt tcataattgt
    33241 cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct tttgctttta
    33301 cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct ttgataaccg
    33361 tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc tcaatcaatt
    33421 cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag aacgcatctg
    33481 ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag cttggtgtca
    33541 gattcaagat gataccaata acaccacctt gctccatttc atggtaagat ttgatggcta
    33601 acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata tctgactctt
    33661 ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt tcattatgag
    33721 taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca tatttttcat
    33781 aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac atcggcatat
    33841 cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca tcaaaataac
    33901 gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt atcaaacggc
    33961 tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac tgtacatcct
    34021 cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca aagaactttt
    34081 gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc
    34141 ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct ggaaattgat
    34201 attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat tccatgtatg
    34261 atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat tcgttttaac
    34321 agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt gattttcgga
    34381 aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat atccttcccg
    34441 attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt gtaattgctg
    34501 acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata gccgcttttt
    34561 ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg gaccgcttct
    34621 ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc aataaatccg
    34681 atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact ttctcgatca
    34741 ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc gataactgga
    34801 atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa gaagacaatc
    34861 agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt ttctggctga
    34921 tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct ttcgatccct
    34981 gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc aatagagaag
    35041 cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa cgctgaaatc
    35101 gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa gtaacgggaa
    35161 actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc catacattct
    35221 cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat gaccgcattc
    35281 tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt agagcaactt
    35341 cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt aatatagcat
    35401 ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg attttttgcc
    35461 tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg tttttctctt
    35521 ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc aaatggggga
    35581 agtattttga agaaattaat cgtactattt attttgtctg cgggactttt gacattgact
    35641 ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc
    35701 ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt acgtaaagat
    35761 atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg
    35821 tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat
    35881 atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa
    35941 acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga tggcaacatc
    36001 tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt ttctgctata
    36061 gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc ctgattattg
    36121 tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa gcttgagcat
    36181 atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat cttatcctgt
    36241 tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc actatggctg
    36301 atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag taaaacaaac
    36361 agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca aatatttgaa
    36421 tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt
//
LOCUS       DASJUF010000015        36454 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJUF010000015 DASJUF010000000
VERSION     DASJUF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.015"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(183..617)
                     /locus_tag="U4B62_000669"
     CDS             complement(183..617)
                     /locus_tag="U4B62_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2140851.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            765..1906
                     /locus_tag="U4B62_000670"
                     /pseudo
     CDS             765..1906
                     /locus_tag="U4B62_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2032..3111)
                     /locus_tag="U4B62_000671"
     CDS             complement(2032..3111)
                     /locus_tag="U4B62_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2140852.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3220..3660)
                     /locus_tag="U4B62_000672"
     CDS             complement(3220..3660)
                     /locus_tag="U4B62_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2140853.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3717..4127)
                     /locus_tag="U4B62_000673"
     CDS             complement(3717..4127)
                     /locus_tag="U4B62_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2140854.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4308..4742)
                     /locus_tag="U4B62_000674"
     CDS             complement(4308..4742)
                     /locus_tag="U4B62_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2140855.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4842..5690)
                     /locus_tag="U4B62_000675"
     CDS             complement(4842..5690)
                     /locus_tag="U4B62_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2140856.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6079..7845
                     /locus_tag="U4B62_000676"
     CDS             6079..7845
                     /locus_tag="U4B62_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN2140857.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7947..9206
                     /locus_tag="U4B62_000677"
     CDS             7947..9206
                     /locus_tag="U4B62_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2140858.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9353..10654
                     /locus_tag="U4B62_000678"
     CDS             9353..10654
                     /locus_tag="U4B62_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2140859.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10657..11508
                     /locus_tag="U4B62_000679"
     CDS             10657..11508
                     /locus_tag="U4B62_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2140860.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11521..12336
                     /locus_tag="U4B62_000680"
     CDS             11521..12336
                     /locus_tag="U4B62_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN2140861.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12487..13653
                     /locus_tag="U4B62_000681"
     CDS             12487..13653
                     /locus_tag="U4B62_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2140862.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13883..14530
                     /locus_tag="U4B62_000682"
     CDS             13883..14530
                     /locus_tag="U4B62_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2140863.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14614..15558
                     /locus_tag="U4B62_000683"
     CDS             14614..15558
                     /locus_tag="U4B62_000683"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN2140864.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGV
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15693..17144
                     /locus_tag="U4B62_000684"
     CDS             15693..17144
                     /locus_tag="U4B62_000684"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN2140865.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17165..18001
                     /locus_tag="U4B62_000685"
     CDS             17165..18001
                     /locus_tag="U4B62_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2140866.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18001..18660
                     /locus_tag="U4B62_000686"
     CDS             18001..18660
                     /locus_tag="U4B62_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2140867.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18786..19475
                     /locus_tag="U4B62_000687"
     CDS             18786..19475
                     /locus_tag="U4B62_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140868.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19481..20437
                     /locus_tag="U4B62_000688"
     CDS             19481..20437
                     /locus_tag="U4B62_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN2140869.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20481..20993
                     /locus_tag="U4B62_000689"
     CDS             20481..20993
                     /locus_tag="U4B62_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2140870.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21249..21797)
                     /locus_tag="U4B62_000690"
     CDS             complement(21249..21797)
                     /locus_tag="U4B62_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2140871.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21886..23067)
                     /locus_tag="U4B62_000691"
     CDS             complement(21886..23067)
                     /locus_tag="U4B62_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN2140872.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23303..24811)
                     /locus_tag="U4B62_000692"
     CDS             complement(23303..24811)
                     /locus_tag="U4B62_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN2140873.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24970..25161
                     /locus_tag="U4B62_000693"
     CDS             24970..25161
                     /locus_tag="U4B62_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140874.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25364..26281
                     /locus_tag="U4B62_000694"
     CDS             25364..26281
                     /locus_tag="U4B62_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN2140875.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26274..27347
                     /locus_tag="U4B62_000695"
     CDS             26274..27347
                     /locus_tag="U4B62_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140876.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27495..28013
                     /locus_tag="U4B62_000696"
     CDS             27495..28013
                     /locus_tag="U4B62_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140877.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27994..28386
                     /locus_tag="U4B62_000697"
     CDS             27994..28386
                     /locus_tag="U4B62_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN2140878.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28462..29397
                     /locus_tag="U4B62_000698"
     CDS             28462..29397
                     /locus_tag="U4B62_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN2140879.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29530..30309
                     /locus_tag="U4B62_000699"
     CDS             29530..30309
                     /locus_tag="U4B62_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2140880.1"
                     /translation="MNKKKSAPWLQALHAVMPLYISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30302..30619
                     /locus_tag="U4B62_000700"
     CDS             30302..30619
                     /locus_tag="U4B62_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2140881.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30863..32473
                     /locus_tag="U4B62_000701"
     CDS             30863..32473
                     /locus_tag="U4B62_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2140882.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32630..33478
                     /locus_tag="U4B62_000702"
     CDS             32630..33478
                     /locus_tag="U4B62_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2140883.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33709..34188
                     /locus_tag="U4B62_000703"
     CDS             33709..34188
                     /locus_tag="U4B62_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140884.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            34221..36449
                     /locus_tag="U4B62_000704"
     CDS             34221..36449
                     /locus_tag="U4B62_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2140885.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
BASE COUNT        12167 a         6505 c         7125 g        10657 t
ORIGIN      
        1 acttcttttt taaaaaaagt attcatcata aaagccacta cttgcggatc ttcatcataa
       61 aattctgtag cagtacttga aaagatgttc caataaatat ctaatgctgc atattgaatt
      121 tgtacctctt ttccagaaaa agactttctg ccattattag aaaaggagat agacaaaccg
      181 tactatgcta aaaatgcatt aatctttttc attttcgtat acaaataaga ggcactatag
      241 tttgtttgct gactgaaact gataatcgaa tttgtctgac agctgaacaa agaagtcaag
      301 atctgataac tgactgattc gttgcagtat cttccaaata gacgataata gcagtaagcc
      361 ggatcaattc cttcaaaatt caactttaaa aaaggttttt gcttatcaat atgcagttct
      421 gttagattga gacgctcaaa gtcttgttct aattctttga tgtatagtaa aaaatttgat
      481 tcagacatat ggaaatgatc tgctaaatat tgtgtagtaa taaagaaacg atgttcagat
      541 aggaaaagaa aaatatctaa ttttgtattg ctttttttct tcaagataaa cgttttaatc
      601 gcttcttgag tttgcatgaa agaggctcct ttttacttaa attgttaaaa cttgaaaata
      661 ttactgtttt tttcataaat ttagacaaaa aaacagtaaa catataggta gaatctctac
      721 tatcttattt cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca
      781 tcttaatatc aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca
      841 cagaggagac agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta
      901 cctttcagga tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa
      961 cagcgaccat caaagcaaaa attgactatt cgggagactt agcacctata ctacaatcag
     1021 ctgttacatt tgagaatgtt gatcccactt tgtcgattgg ctatgacgag aacactttga
     1081 ctatccaaca caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa
     1141 aaccagcgct ttttactgtc aaggtatcag atggactacc gaacgatcat catttaacgc
     1201 cgtatagtca gcggcagaca attgagcaag attcagccat cgactcagga ggagatcttg
     1261 ttgaagaacc aacagataaa ccagaaaatg aaaataagcc agaagtaccg cctacagaaa
     1321 atccagacgg agaacagaaa ccggaaatag aacctggaga agaacccgat acagaaactc
     1381 aaccagagcc agacaataaa tcaaaacctg aaataacccc tggagaaaaa ccagatgtaa
     1441 ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag
     1501 ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa ccagatgtga
     1561 ctccagaacc agatacggac gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa
     1621 caattcaaga ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa
     1681 atgaagagca gctgaataaa aaatcacgaa tatttgatag cgcccaatca gctgaaacct
     1741 taaaaagtag caaggataca acctttgctg tcctgaaacg aaaaataaac agctaccaaa
     1801 atccggagag tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc
     1861 aacaatgctt tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt
     1921 ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa atgaccccaa aataccagat
     1981 tgaaatctga tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat
     2041 atctgtttct tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa
     2101 ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag
     2161 taagaccaca aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt
     2221 cagcacataa gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc
     2281 tcggatgcct ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc
     2341 tgctactggg atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa
     2401 gccaaataca gccaaggcga ttgtagtgag caacgtattt aaaagagcga caacaaattg
     2461 agcttctaat accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg
     2521 aaagaaccaa gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt
     2581 ttcaatcata aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc
     2641 tatatcctgt aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc
     2701 gtttcgctca atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg
     2761 ctcgtaaaaa tcaataacgg actggatcat atcaaatgtt tgatgaataa gcatcgggat
     2821 atactttgtg aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa
     2881 aacacctgta gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata
     2941 agtaaagata aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa
     3001 aacgacaaat aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata
     3061 aacggtcaaa tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg
     3121 gaatatgaaa ataagaaaga gaaagagcaa aaagaaaaac cgctaaatga cgttatctta
     3181 tgagttggtt ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg
     3241 atgaaaatta tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc
     3301 aagtggaaga taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc
     3361 accttgagga gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt
     3421 aatataattt ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca
     3481 caaatcattt cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac
     3541 aagatactgg gtataggtaa gattcagcgg cttgaggagg gaagtgtagt gtctaatgat
     3601 ttccttagag gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat
     3661 ttttccacct ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat
     3721 tcactaactg ctttcagagt aacttctata tttccagctg tagcttttga atacggacac
     3781 actttatgcg ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat
     3841 ccttcaatct ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt
     3901 gcactgacag tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg
     3961 ttaaaacatg cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg
     4021 cgtttaccag gagctgcaat attcaattcg aatgtacgat cgggattatg aacttctcca
     4081 gagcgtccgc ctgtgttgat cattttcgta gaatacattt ttttcataaa attaccacct
     4141 ttaattatat tttatacaat tgaattttac acaatataat tgtggaaagc aaagaaatgg
     4201 attggcataa gataaataag agaaagcaca tgaaacagac gcgctttctc gaatagcaaa
     4261 catatacaaa aaaatatcag agacaaaaac taatctcatc ataaacatta catatatttc
     4321 tcaaaaagtt gttttctttc ttcaaaactt acttcacgat ggacctgatg aagcatccat
     4381 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
     4441 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
     4501 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
     4561 agagacgtgt tttcatccag catatggaat ctcgtgccat acaaggtaaa aatgacttca
     4621 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
     4681 tacagttcta gtgctttcaa actatcattt aatacaaaat ctatttccgt tcctttatgc
     4741 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagtta
     4801 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
     4861 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
     4921 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
     4981 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
     5041 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
     5101 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
     5161 gataaaatag cataaagaaa ctcttctatt ccaaaaacaa acagattaag agaaacaacg
     5221 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
     5281 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
     5341 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
     5401 tctggaacta ttgcaatgat taaaggaaaa aacaagcttc cgaaaagtac tttaaaaaag
     5461 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
     5521 actatagctc ggtcaaatcc taaaacagct tccagtaata ttcctagacc gctgacacct
     5581 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
     5641 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttatccactt
     5701 ccttaattca ttaataatac tcttatctta ttataactta cttttcacct taagttaact
     5761 ttaattaata aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac
     5821 ttctacgtta aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt
     5881 aagataaatt caggacacta aatagtacag gaagtagatt aaaactttta aaagttttaa
     5941 gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata
     6001 acatttttga aagcgttgtc ttttgtttct ctatcactta tagtgatgac gtcatattaa
     6061 aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat
     6121 gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc
     6181 aaaaaagtat atgtgctttg tggagatcca tataccataa caactgaaaa atggtatcaa
     6241 aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa
     6301 gtgacgagcg aaacaaggcg cttacagtat gcgttccatg ttgttgggga agataacacg
     6361 gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca
     6421 aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg
     6481 gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca
     6541 aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga agacttcttc
     6601 gggggagatt tgcaaggtgt gagggatcat ttggattact tgacggacct tggcgtcaat
     6661 ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat
     6721 tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa
     6781 gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat
     6841 tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc
     6901 cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa
     6961 aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat
     7021 ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt
     7081 gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga
     7141 gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct
     7201 caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt cacagattcg
     7261 attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat
     7321 catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct
     7381 catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg
     7441 atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg
     7501 atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa
     7561 gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag
     7621 attatttctg aaggttctac tcagtggcta atagtatatg atcgtgaaga taagctctat
     7681 ttcacacggg aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct
     7741 tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac
     7801 gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta
     7861 acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca
     7921 cgttgaatac acaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag
     7981 ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag
     8041 cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag
     8101 attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag
     8161 tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg
     8221 gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc
     8281 tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga
     8341 cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata
     8401 acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag
     8461 acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga
     8521 ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg
     8581 atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt
     8641 atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag
     8701 gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat
     8761 ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc
     8821 taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt
     8881 atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc
     8941 aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag
     9001 caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag
     9061 cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg
     9121 atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca
     9181 gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg
     9241 gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg
     9301 ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca
     9361 agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat
     9421 ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct
     9481 attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg
     9541 aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat
     9601 cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc
     9661 gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat
     9721 aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac
     9781 gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca
     9841 tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt
     9901 tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt
     9961 atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa
    10021 atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc
    10081 ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac
    10141 tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa
    10201 aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc
    10261 ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc
    10321 agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt
    10381 cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc
    10441 catgatctat ctgtttaaca atggtggtcc cggaagcgta ggcggaggag ctggttctac
    10501 tgatattctt atttcctgga tctataaact tacgactgga acttctccgc agtattctat
    10561 ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt
    10621 taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct
    10681 cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc
    10741 attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca
    10801 gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt
    10861 gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc
    10921 gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc
    10981 cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg
    11041 actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca
    11101 atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac
    11161 actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt
    11221 tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct
    11281 tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat
    11341 cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct
    11401 ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta
    11461 caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc
    11521 atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg
    11581 ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg
    11641 atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa
    11701 tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa
    11761 ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg
    11821 gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag
    11881 aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat
    11941 tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta
    12001 gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt
    12061 ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg
    12121 atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg
    12181 ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac
    12241 tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt
    12301 gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca
    12361 atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac
    12421 ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg
    12481 aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct
    12541 gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc
    12601 caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcttct
    12661 ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca
    12721 ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa
    12781 tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat
    12841 tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt
    12901 gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat
    12961 atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg
    13021 ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta
    13081 agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa
    13141 tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca
    13201 agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta
    13261 aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc
    13321 ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt
    13381 actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta
    13441 cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca agtccaagat
    13501 caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct
    13561 tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct
    13621 ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact
    13681 atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta
    13741 ggattgtctc taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct
    13801 ttaattttta aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt
    13861 tataacaaag gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc
    13921 tttgttttta gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc
    13981 cagtagcgaa cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc
    14041 gtctagccag acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc
    14101 agctcgtgcg atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga
    14161 attagatagt gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga
    14221 atacgaagca agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct
    14281 agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt
    14341 gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac
    14401 agattgggat ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg
    14461 caatcaaagt acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca
    14521 agatgattaa gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact
    14581 agacaaaata acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat
    14641 catatctttg ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa
    14701 ttagatgaac tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat
    14761 gaacgaaaag gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct
    14821 atgggacttc caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa
    14881 gtgcaggaaa accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa
    14941 aacttggaaa tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat
    15001 ttatcttgtc ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat
    15061 gaaacactga aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct
    15121 tattttgatt ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc
    15181 tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca
    15241 gtagtcatca aaccaaaaga aggggtcggc ggcattggcg gagagtatat caagccgact
    15301 gctttggcga atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt
    15361 acaggtggga ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg
    15421 ttacagattg gtacagagct tcataaagaa gggccggaaa ttttttctag aatcataaaa
    15481 gaattaacgc agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa
    15541 ttacgcacca tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata
    15601 aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt
    15661 gggaaaataa cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag
    15721 agcttaaaga aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa
    15781 agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc
    15841 acgagctatt gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa
    15901 aaatgctcgg tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa
    15961 cacaccgagc ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc
    16021 cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct
    16081 atggcccagc aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag
    16141 ctgctgctgc agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt
    16201 ctattagaat acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga
    16261 tgccagttgg tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga
    16321 cagtttctat gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg
    16381 cgccatatca agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga
    16441 tagctttttg tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg
    16501 taaaaaaagc tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc
    16561 cactagatgg tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag
    16621 caatgatccg ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac
    16681 ccatttcttg gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt
    16741 ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc
    16801 tattcctttc accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg
    16861 atgcgataag agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg
    16921 ttgtttcttc catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc
    16981 tgactggaca accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag
    17041 gaatccaatt tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat
    17101 ttgaagcaaa tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag
    17161 aagaatgaag aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc
    17221 ttgcggtaca gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa
    17281 agaattagta gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa
    17341 tgaattagtt ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa
    17401 aatctccttt aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc
    17461 agattttgct gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc
    17521 agagactttc aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat
    17581 ccactcactt gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat
    17641 gcgtttagcg gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca
    17701 gtatttaagc gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca
    17761 gaaaatggcc gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt
    17821 caatgaaaac gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat
    17881 cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata
    17941 ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg
    18001 atgtactttc ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa
    18061 ggcttggtat acacattagg gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg
    18121 ttgcttttga cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt
    18181 tatttatcgt tctttagagg cattcccgca ctagtcctgc tgtttctgtt atattttggg
    18241 ttaccttatc aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt
    18301 gcatttattg gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac
    18361 gctggtttag cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa
    18421 gcttttcgaa tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact
    18481 tcgttagcag caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt
    18541 cggacattcg attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt
    18601 atcgggatcg aaaaagtgca acagtattta gaaaaacgtt ctatgcatca gtcaatctga
    18661 aaaatagaaa tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc
    18721 tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag
    18781 aaaacatgaa gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg
    18841 agcaaggcgg cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata
    18901 cgcttagctg cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag
    18961 tgacaaaagc agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt
    19021 ttgttttacc aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg
    19081 ttttagtgta tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga
    19141 ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct
    19201 tcatggcttc tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat
    19261 attttccaac agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat
    19321 tttctaacga acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg
    19381 agttgcaaga agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc
    19441 agatccgtcg gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag
    19501 aggaaaacaa tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa
    19561 taaaatccaa ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga
    19621 attatttcaa aaaaagcacc atcgaatcgc tgtgatccaa aatccccaag taggacaatt
    19681 caaattattc gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc
    19741 tgcacagatc ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct
    19801 tgttttcgat ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt
    19861 tgattcgaaa tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa
    19921 aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac
    19981 agatgacttt tttagtgatg actatctttt tttccacgca gacggatata ttggattttc
    20041 tgatttcacg atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc
    20101 tatccatcta acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga
    20161 cagtaacgac tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa
    20221 aaaatggatc atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt
    20281 ggctctttac cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat
    20341 gcatcatttg gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta
    20401 ttcaaaagtt caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg
    20461 aatagaaaag aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag
    20521 tggtagaaat cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc
    20581 aaaacgggca gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct
    20641 atgtattaat agtggaagat caaattgctg gaacagctgc attagtctca agcgtggatc
    20701 ccgtttacac aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc
    20761 atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac
    20821 atttgttgac gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag
    20881 ctaatgaacc gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg
    20941 aatttccttt tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa
    21001 atcatctgag caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca
    21061 agaggttgta acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc
    21121 tcaaattttt gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc
    21181 atcgagaggt cgtgacatag cttttgtcac gacctctcgg cttatcctac gagtattatt
    21241 cgatacattt atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca
    21301 tcgtttgaca cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct
    21361 tctacataaa ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg
    21421 cgcgattgca atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa
    21481 tactcatata aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc
    21541 agtttctttt taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac
    21601 tctgcaaact catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa
    21661 tcctcttgat ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt
    21721 tccggctgaa aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat
    21781 acatagcaat aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg
    21841 aaaatatttc caaagctttt aaaagtaact ttgctttttt gtatattata ccagcaagcc
    21901 agtcacaata gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg
    21961 ttttgcgaca agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc
    22021 gatagaagag aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc
    22081 agtaaaatgg aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag
    22141 tttagtcgcc atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa
    22201 ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg
    22261 gaagatagca ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt
    22321 gatcgccaca tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc
    22381 tttgttttct tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat
    22441 tgaagccaga atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga
    22501 atcgatcagt gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac
    22561 actcatggtg aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa
    22621 aacttccgat tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat
    22681 cccgttgatt ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat
    22741 atagcgtaga ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa
    22801 gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc
    22861 aaaagcaaga agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt
    22921 tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt
    22981 caatttgatt gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa
    23041 accaataata cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt
    23101 tacctgtttt gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg
    23161 aaacgcattc attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta
    23221 acgtcttagt aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc
    23281 aacatttata agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg
    23341 cgcaggtaat atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca
    23401 cagtagagtg ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga
    23461 ttggattcgt tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat
    23521 ccaaagagga atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg
    23581 acaataaagg aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg
    23641 ccgctgacgc atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt
    23701 cgattgcaat cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat
    23761 agtcatgggc agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa
    23821 tcagtttgat tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg
    23881 tttggaaata acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca
    23941 cgatcccaaa tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt
    24001 taccagtaat tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat
    24061 agttcacagc tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt
    24121 ttagatcgac tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc
    24181 atactgggaa agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat
    24241 acagttggtt caaggaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa
    24301 cgattggttt agttgtttca atatactcat tttttccaaa accatcttct gagacgattt
    24361 tttctacgtt gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc
    24421 tttctagttt attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga
    24481 cactgttgat taataagcca taaccacgta attcatcgat taaccgcaag atatccatat
    24541 cataagtaat cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc
    24601 aaatcagtat ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt
    24661 caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac tagcttccca ccaaattcta
    24721 aatataattt gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat
    24781 atttttctga atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt
    24841 ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa
    24901 aatggtttaa accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga
    24961 aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg
    25021 atttttaccg gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact
    25081 tatctaaacg aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca
    25141 agagatctgc ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat
    25201 cagatggaaa gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt
    25261 tatggagcat tgcccgctct tttttataac aattttttac agaaaatggt actattgaag
    25321 tattaataag tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac
    25381 aaatgcaaat tatgagccag acgaactaga ggagacgaat gcggatcagg atacaggaca
    25441 agaatcgtct tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt
    25501 agaagactta caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg
    25561 tttatacaat ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat
    25621 cgatcaattg ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga
    25681 acaagtagaa aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga
    25741 tcggcccacg gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga
    25801 tgaagagtgg gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga
    25861 gctagacgca aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa
    25921 agaaatcaat cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa
    25981 aatcgaggaa gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt
    26041 aggctggctt ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa
    26101 gcaagaagca agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat
    26161 cagacgctat ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa
    26221 ttatcttggc aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg
    26281 aacacgatga agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga
    26341 acacttcatt ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc
    26401 tagaggaaac aaaaatacac gaaaaagaaa aagtaactcc gacgaatacg aatactgcag
    26461 aaaacaccca tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag
    26521 atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta
    26581 tgagaaaaga tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta
    26641 tattgaatga agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt
    26701 atgaattact gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca
    26761 catggaaaac aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct
    26821 tttcctttgt attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa
    26881 acgtatcacc agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc
    26941 gacactggtc agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat
    27001 tgaatcaatt attagcccat gcaagaacac ttggttttac cgttaatcag acattactag
    27061 acaatacgaa gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat
    27121 tggatgatat ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg
    27181 aaactactta tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa
    27241 aagatcaaac agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct
    27301 ttatcaatta cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg
    27361 attaccagat gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata
    27421 attgtcacaa gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag
    27481 ggatttcatt aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt
    27541 gtgtagcagg taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt
    27601 ttttatttat agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta
    27661 ttgcgatcag cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct
    27721 atactgttgt tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc
    27781 tatggttaaa gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg
    27841 tttttgatac gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt
    27901 cgtttgattt gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg
    27961 tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt
    28021 gcaatgagtg tcagtatact cattttgaca agttgcagta caacctatac acaatcggct
    28081 tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat
    28141 ggtcaaaact tttcggaaaa agaagtgttc ttcaaaaaag gcgatgattt actgagtatt
    28201 ttgaaacgaa attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac
    28261 tcacaggatg agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact
    28321 actggtgcca aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa
    28381 ctataagttt cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt
    28441 tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt
    28501 gctgctctag gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt
    28561 ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt
    28621 ttgctgttat tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt
    28681 gaaggacgag caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt
    28741 ggggcacttg gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt
    28801 aatccgatca ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta
    28861 cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt
    28921 ttgcgcgaaa gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta
    28981 atcggtttgg tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca
    29041 tttgatgcga tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta
    29101 gcaaaagaat tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa
    29161 atcaagttag cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat
    29221 ttaagtccaa cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat
    29281 gaagcttact cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag
    29341 aagatgtata tatacattga aacagataaa tatgacccaa attattctcg taaataaaac
    29401 gtttattcat gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg
    29461 ctacaattta gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga
    29521 gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg
    29581 ccactttata taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt
    29641 ggatttgatc ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag
    29701 tttttagttg catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt
    29761 ttctttttag aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa
    29821 gaaaagcgga cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg
    29881 aactacttaa agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat
    29941 tggttctcaa tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg
    30001 attcgagtag atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt
    30061 atcatgcaga tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg
    30121 gtcgtattca tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct
    30181 tcatttgccg gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga
    30241 gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac
    30301 catgactaaa ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc
    30361 gcgcttgttc ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg
    30421 gatgaaatat gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac
    30481 tcatgaacat ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg
    30541 cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt
    30601 tttgctgtct atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt
    30661 gttcaaactg atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc
    30721 gtagggataa aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt
    30781 agagagggat ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga
    30841 aagaatagtt gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac
    30901 ctttgctaat cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat
    30961 gatggggaac aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc
    31021 ttatggattg ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt
    31081 agctgtagcc acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat
    31141 caatatcatg tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt
    31201 actagccgga atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt
    31261 atgcccatct ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa
    31321 aaatgatctg cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt
    31381 tatcttggct ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt
    31441 attgatcagt acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat
    31501 cgtcggacgc agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt
    31561 cgaaagagaa aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt
    31621 tgtcggctca gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct
    31681 tcactttttc cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc
    31741 tgtcaatcca aataaattga cattgatcct gatctccatg gtaggaacag tagctataag
    31801 cagcttgcca atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt
    31861 aggagatagt ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt
    31921 attagctgga ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt
    31981 ccaaatggct ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct
    32041 tcaatctttg aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa
    32101 agtcatcttc caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa
    32161 tggcttagca ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg
    32221 aatagcacct ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat
    32281 tatgcttgca gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc
    32341 aaaaagctat gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt
    32401 attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag
    32461 aagcaaagaa taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat
    32521 gattacgctt gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga
    32581 tatagttata aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc
    32641 agtagttaca gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt
    32701 cgttattcca atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga
    32761 agcggatgaa tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc
    32821 tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat
    32881 tttaagtatc catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa
    32941 agatgacatc agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat
    33001 gggacacatg gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat
    33061 acttgcacat atcgatcgaa tccgagataa tacctacgct tatctgattg tagatgattt
    33121 gaacaattta gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt
    33181 aaaaatcaaa ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg
    33241 ttcaagtaaa aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat
    33301 acaaccgcca gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga
    33361 gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc
    33421 agtcattggt acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt
    33481 ccttttattc aaaaaacata ccaaaaatat tccaacatca tcttggactt tctacatgtt
    33541 actaatgagc gtgtaaaaga acgagatggt gtttttattt ctatcaatga agctcttttt
    33601 ctttgcaatc ctcacttcaa tcctctataa tgtcgcatat agtttagtaa atgagtaaac
    33661 tactaattta cttatttact aaaaataaaa ggatgaggag ttaaaataat ggaaatacca
    33721 acaaatttaa aacataaacc agtaatcgtt gtagaagact atgataaagt tgatggaaga
    33781 aatgcattaa atacggatgc aaaagggctt tcactgggcc ttgctcagtg gaatgatcgt
    33841 ggaaaagtcg atatctcagg caaggtatgg cgacatactg gagaaaaatg gtcacgacag
    33901 tcggaagaat taccaatcac acgtatttta gatttagcga tcttagctgc acaaggaagt
    33961 ttatacttcc aagatgctta tcgttatgaa aaattttatg atccagaaaa tccagtcgta
    34021 gacatcattg gattacaagg gaaccgaatg acggtagaag tagataccaa aaatccaaag
    34081 atcgatgaag acatcatttt gtattacgat acactacaaa aagacggaga actgattggg
    34141 gaacgattcc gtattttaaa acgtctttta gatgagctag gatattaata gacagagatg
    34201 atcaaaaggg gaagagaagc atggagaatt tctcactaac agaaaaccga accttttcac
    34261 ggctagaaaa gcaaatgatc tggaagaaac cgaaaagcca tcggacaagt gcagaagaat
    34321 ggcggatcgc ctccgaaatc aaagggaatt ggcaagatcc tgaaatgaaa atcagtaacg
    34381 tacttttgga aggagatgcc gggtcgggga agacacaatt agccaaagca ctatctgctg
    34441 atctgcaact tccctataca aaagtgacct gttttgcaga tatggacaaa tcagatgttt
    34501 tcggagcctt attacctgta gtagattcta ataaggaaca agaccaagaa ttactagaag
    34561 ctatttacca aacagataca cttgaagctg tactgacact gatccagaac cattacggca
    34621 ttgatccttt ttctgctaaa gaaaaattag cagagctagt tcaaaggatc gaagctgatt
    34681 atccagatgc aatccattac agctattacc cttctgaaat cgttcgagcg attgaaaaag
    34741 gatatttatt agagattcaa gagccgactg tcatccgaga tgcctctgtt cttgtggcac
    34801 ttaactcagc gctcgaacca aatgggatgt tgaatttacc gacaggaatc attcgtcgcc
    34861 atccagattg cgtcattgtg atcactacaa atcgaaatta ccaaggaaat cgcccattaa
    34921 atgaatcttt aagagatcgg atgcaacatg cggaaaaaat ggatcttcct gccttagaag
    34981 tcatggcaga acgtgcgatg gcgaaaacta tgatccaaaa acaagaactt ctcttaaaga
    35041 tggcagagat tatccgtttg ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag
    35101 caggaatgcg ttcttatttt tattgggcaa atacctataa gcaaggacaa gatttactcc
    35161 agtcgattta tccaaaagtt ttgtataaat tatccactga ttcagatgaa ttggcaattc
    35221 ttgaacaagc tttgtctgac agtggattgc tactggaact tcaggacata ttgagaaaag
    35281 aaaaatgggg tcatcctcaa tctgagcata caaaaggaag aaccattagc gccgaagaag
    35341 caaatgaacg aaacatcact gaaaatgctg aagaaattct gcgcttagat gaaaagaaat
    35401 cgttgtctgc tgaagaacca aatgagggag aagagaaaca agaagaaaca atagaaaaaa
    35461 acgagattcc tcctcaaatg caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa
    35521 aagaacaaca aacaggagat gattctcaag aaatgtctgc ttctgacatg gaagcacatg
    35581 ataaggaaat caagaagcaa ctgaataaag aagcgcgaca aatcatgaag gaaacgattc
    35641 atgaaaaaga aggtctcatc gttcaccgac cagctttcca aggaaaaacg ccggaagcac
    35701 gtgagctaca catgcaagta atgccaatag tagaatcact tagccggcaa attttagagt
    35761 tactggataa tgaacaaaca gagacgtacc aaaaaggaaa atacgaaggt cagcggttca
    35821 atgcaagcaa agtagcatat ggtgatttga gaaattttga caaaaagaac ccgccacatg
    35881 agcaaccttc tttagcggta gctgtacgga tcgatgaatc tgggtcaatg atacgggatg
    35941 atcggatcga agcggcaaaa aaagcagcga ttgccattgc cgaattttct aagaaagtcg
    36001 atatcccatt gttgatttac ggagatacag cagaccgttc gtcaagagaa aagacttctt
    36061 tgttttctta taaagaattt gaagacggat ttcaatggtt aaatgaaaaa ttagtcacta
    36121 tgaaaccaag gcaaaacaat cgtgatggtg ctgcacttcg tttagcagca gaaaaattaa
    36181 acagacaatc cgcaacaacc aaactgttgc ttaatattag tgacggtcag ccaaaagcgt
    36241 tgccggatta tacaggcaaa aaagcaaaag aagatatcca gtctgttttg aaagaatatg
    36301 aacgtcaagg aatcctattt gtctccgcag ctattggcca agacaaagaa gaaatcaagt
    36361 cgatctacgg tgaaagtcgt tttgtagata ttactgactt gaatgaattt cccaagcaaa
    36421 tgattcaact gattgcccgc tatctatgat aaaa
//
LOCUS       DASJUF010000016        36424 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJUF010000016 DASJUF010000000
VERSION     DASJUF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.016"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1586)
                     /locus_tag="U4B62_000705"
     CDS             complement(99..1586)
                     /locus_tag="U4B62_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140886.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
     gene            complement(1909..2388)
                     /gene="rlmH"
                     /locus_tag="U4B62_000706"
     CDS             complement(1909..2388)
                     /gene="rlmH"
                     /locus_tag="U4B62_000706"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN2140887.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2816..4105
                     /locus_tag="U4B62_000707"
     CDS             2816..4105
                     /locus_tag="U4B62_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN2140888.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4144..4749)
                     /locus_tag="U4B62_000708"
     CDS             complement(4144..4749)
                     /locus_tag="U4B62_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN2140889.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4954..5418)
                     /locus_tag="U4B62_000709"
     CDS             complement(4954..5418)
                     /locus_tag="U4B62_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2140890.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5440..5760)
                     /locus_tag="U4B62_000710"
     CDS             complement(5440..5760)
                     /locus_tag="U4B62_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2140891.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5904..6983)
                     /gene="pepA"
                     /locus_tag="U4B62_000711"
     CDS             complement(5904..6983)
                     /gene="pepA"
                     /locus_tag="U4B62_000711"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN2140892.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7183..7533
                     /locus_tag="U4B62_000712"
     CDS             7183..7533
                     /locus_tag="U4B62_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2140893.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7756..8703
                     /locus_tag="U4B62_000713"
     CDS             7756..8703
                     /locus_tag="U4B62_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2140894.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8760..10178)
                     /gene="pepV"
                     /locus_tag="U4B62_000714"
     CDS             complement(8760..10178)
                     /gene="pepV"
                     /locus_tag="U4B62_000714"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN2140895.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10327..11172
                     /locus_tag="U4B62_000715"
     CDS             10327..11172
                     /locus_tag="U4B62_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN2140896.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11176..11616
                     /locus_tag="U4B62_000716"
     CDS             11176..11616
                     /locus_tag="U4B62_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN2140897.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11666..11860
                     /locus_tag="U4B62_000717"
     CDS             11666..11860
                     /locus_tag="U4B62_000717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140898.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            11996..12682
                     /locus_tag="U4B62_000718"
     CDS             11996..12682
                     /locus_tag="U4B62_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2140899.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13090..13779)
                     /locus_tag="U4B62_000719"
     CDS             complement(13090..13779)
                     /locus_tag="U4B62_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140900.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13754..15883)
                     /locus_tag="U4B62_000720"
     CDS             complement(13754..15883)
                     /locus_tag="U4B62_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN2140901.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15931..17268)
                     /locus_tag="U4B62_000721"
     CDS             complement(15931..17268)
                     /locus_tag="U4B62_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN2140902.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17520..17879)
                     /gene="rplT"
                     /locus_tag="U4B62_000722"
     CDS             complement(17520..17879)
                     /gene="rplT"
                     /locus_tag="U4B62_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN2140903.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17932..18132)
                     /gene="rpmI"
                     /locus_tag="U4B62_000723"
     CDS             complement(17932..18132)
                     /gene="rpmI"
                     /locus_tag="U4B62_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN2140904.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18204..18728)
                     /gene="infC"
                     /locus_tag="U4B62_000724"
     CDS             complement(18204..18728)
                     /gene="infC"
                     /locus_tag="U4B62_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN2140905.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18772..18897)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19048..19680
                     /locus_tag="U4B62_000725"
     CDS             19048..19680
                     /locus_tag="U4B62_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN2140906.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19682..20587)
                     /locus_tag="U4B62_000726"
     CDS             complement(19682..20587)
                     /locus_tag="U4B62_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2140907.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20672..21331)
                     /locus_tag="U4B62_000727"
     CDS             complement(20672..21331)
                     /locus_tag="U4B62_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2140908.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21647..22591
                     /gene="mvk"
                     /locus_tag="U4B62_000728"
     CDS             21647..22591
                     /gene="mvk"
                     /locus_tag="U4B62_000728"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN2140909.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22584..23561
                     /gene="mvaD"
                     /locus_tag="U4B62_000729"
     CDS             22584..23561
                     /gene="mvaD"
                     /locus_tag="U4B62_000729"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN2140910.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23574..24659
                     /locus_tag="U4B62_000730"
     CDS             23574..24659
                     /locus_tag="U4B62_000730"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN2140911.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24656..25720
                     /locus_tag="U4B62_000731"
     CDS             24656..25720
                     /locus_tag="U4B62_000731"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN2140912.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25969..26772)
                     /locus_tag="U4B62_000732"
     CDS             complement(25969..26772)
                     /locus_tag="U4B62_000732"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN2140913.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26792..27634)
                     /gene="kduI"
                     /locus_tag="U4B62_000733"
     CDS             complement(26792..27634)
                     /gene="kduI"
                     /locus_tag="U4B62_000733"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN2140914.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27650..28657)
                     /locus_tag="U4B62_000734"
     CDS             complement(27650..28657)
                     /locus_tag="U4B62_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN2140915.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28664..29308)
                     /locus_tag="U4B62_000735"
     CDS             complement(28664..29308)
                     /locus_tag="U4B62_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN2140916.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29408..30166)
                     /locus_tag="U4B62_000736"
     CDS             complement(29408..30166)
                     /locus_tag="U4B62_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN2140917.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30293..31444)
                     /gene="fucO"
                     /locus_tag="U4B62_000737"
     CDS             complement(30293..31444)
                     /gene="fucO"
                     /locus_tag="U4B62_000737"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN2140918.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31526..31837)
                     /gene="rhaM"
                     /locus_tag="U4B62_000738"
     CDS             complement(31526..31837)
                     /gene="rhaM"
                     /locus_tag="U4B62_000738"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN2140919.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31839..32684)
                     /gene="rhaD"
                     /locus_tag="U4B62_000739"
     CDS             complement(31839..32684)
                     /gene="rhaD"
                     /locus_tag="U4B62_000739"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN2140920.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32698..33954)
                     /gene="rhaA"
                     /locus_tag="U4B62_000740"
     CDS             complement(32698..33954)
                     /gene="rhaA"
                     /locus_tag="U4B62_000740"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN2140921.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33951..35411)
                     /gene="rhaB"
                     /locus_tag="U4B62_000741"
     CDS             complement(33951..35411)
                     /gene="rhaB"
                     /locus_tag="U4B62_000741"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN2140922.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35564..>36424
                     /locus_tag="U4B62_000742"
     CDS             35564..>36424
                     /locus_tag="U4B62_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN2140923.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRK"
BASE COUNT        10575 a         7683 c         6695 g        11471 t
ORIGIN      
        1 aaagaaagag gtaactacat aaactaatta aaaaagctaa cttcatagtt ggaggataaa
       61 tggaagaaac ttcaatgatt gtttctcctt gtgttccttc aatcgacact agtcctatta
      121 tcaatctctc ttttttgtcc atttactttt gcgactgttc cccaataatc aaatacagga
      181 agcgttattt tttgtccttt agattcatgt actgtataga ctattttcga atccatcact
      241 tgataaggca tagtatgcca ttccttgcca attcttactt catgattctg caaatgttct
      301 ttgtattgta atgcttccgt gggtgcatag tcataaagtc ctccgggaag gagtgtcgtg
      361 taacgaataa gattttcttg attcattacc aagtgatctc tctgagtgac aactttatgt
      421 tcatagtttg cttttgttgc agcagataca gccaacagta ataaaacaat tgccgttata
      481 ttaactactt ttctttgctt agctgatgtg ctcttcatga aaacgaacga taaaatcaac
      541 gagccaaaca gtacttgaaa gcctaagatt cgccaaggaa attgaatcat ttgaatgggt
      601 gtattttgta ataaagacca tggaaaaaat ttggtggata agagccaaga gactaccgca
      661 ctaaggtaaa tcactttttc tttgcgatta aatcctttaa aaaagaagag tggacagatc
      721 aaagaaataa taccaatgat tcctaccgta aaggttgtca ggtcattatt catcgcacca
      781 atcaaactat ccgagacatt taatgcttgg tgttgtaagg tcgttttatc tggctcatta
      841 atcgattgaa ccatgatttg tcggaacatc ggataccaaa agtatgctgt taaaagaatc
      901 gtcattccta ctgcctttga caactccaca atcaaccgga tgctttcttt ttttcttgtt
      961 tcacttacta caaagccgcc taagactagt aagccaacgg ctattacact cataaaagtg
     1021 gaaagaacat gtgtataaat gagtaagctc atactgactg ctaatggaat tgcacatggc
     1081 tttttctcat aaatcagtgc gtaagcataa tacataacta tcggtataac cgtaagtgca
     1141 atcgcttcag caatagcaga acgatgataa atatcaatcg tacggtaccc ataaaatgta
     1201 tataaacagc taaatataaa agccgcatcg attcttttta aaaattttag tccataatga
     1261 aacatcagca tgatagtaca aacattcaat agccaaacat ataagatata actaacgatt
     1321 aaattattac ttatccaata aaaaatgact gccggaaaaa aggtcaagta gggataaaaa
     1381 taattcactc cgctcccata atgattaaac gtcgtaaaat tgattggacc agaaaaaatt
     1441 gaagataacc ctttgactct agcaaaatgg aaggcaatat cccccgatgt agaaatagta
     1501 tgaacatttc taaaataatt gattgaataa agtccagcaa taattgctag gagaatggac
     1561 aatattattt ttttctttac ttgcattact taattatata ctcctttcat ggattgcttt
     1621 atctagtata acttaaattt taaataaatc gttatttatt tttactctca cttaaaaaag
     1681 cactaaaatt ataagagagg gaggtaatgg tctgaacacc aagaattgtt ccaacatcga
     1741 attgcttaac taatgagttc aaagcttaat tatgtggctc ttatatttac cgagcgtgga
     1801 ttgcgatctc tttagcgaaa caaacatctt tcataaaaaa aacagccata tccgctttct
     1861 tggatatgac tatttactac tactttacta tctttcattc ttcttgtctc acttatgata
     1921 aggcgccccc gcattgatcc ggaaggcacg gtagatttgc tcgacaagga tcagacgcat
     1981 caattgatgt ggatacgtca tttttccaaa agagatctga gcattgcttc ttttcatgac
     2041 ggcttcgctg agtcctaaag agccgccgat aacaaaaaca aggtggcttt ttccttggat
     2101 acctaattgg tcgatttgtt tagcaaaaga ttcacttgta ggatttttcc cttcaattgc
     2161 taaggcatag acgtattctt gttctttgat tttcgctaag atccgttccc cttctttttc
     2221 ttttacttgt ctcatttgcg cttcgcttaa attctccggg gctttttcgt caggaacttc
     2281 gatcaattcg atttttgcat aggcattcag ccgtttgaca tactcattga taccttggat
     2341 caggtatttt tcttttaatt ttcctactga gataattttg atattcatgt tttctctcct
     2401 ttggtcttca tgctcacttt gtttattata atcaattttt tcgtctatat gtttattttg
     2461 gctatttttg ttaaaaagat ttccccaaca gttatgcaca acatccacag gtttatccac
     2521 agaaaacagt gttattttac cttatttcat cacattcatt aggcaaacat acgttttata
     2581 agaatttatc cactttatac acaggctgtg gatatgtttt atcccttatt tttccaatcc
     2641 tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa tcaaatattt tcatctgaaa
     2701 acaatgattt ttcctaaagt ttttcataaa aagttcacag tctcaaagta gactaaagtc
     2761 aacaaataag actatactaa cagtagataa atgaatgtaa ttggaggagg aacacatgga
     2821 tagaaaaaat gtgacaccaa agatgaaaaa aagtaaaaat aatagcctct ggcgtaagtt
     2881 aggtctgggg cttgttggag ggatcgttgg aggactactg acagctggca ttttttatgc
     2941 agcgatgggc actggcaaca atagttctac tgctacaagc ggcaatcaga attctgccgg
     3001 tgaaacagtt gtggaaaatg tcaaagtcaa tgtagattct gatatcacca aagcggtaga
     3061 taaagttcaa gacgctgtcg tttctgttat caacttacaa agtcaaaacc aatcaagcgg
     3121 ttttggcgga ctattcgggc agcaagaaga aagtagcagc tcagatgata gtaatttgga
     3181 agcttatagt gaaggaagcg gcgtgatcta caaaaaagac gggaatactg cttatgtagt
     3241 cacgaataat cacgtagtag atggccagca aggtcttgaa gtcttgatga aagacggtac
     3301 gaaggtaaaa gctgaattag ttggaacaga tgcttattca gatttagcgg tgttgaagat
     3361 caattcagac aaagtcgaaa cagttgcctc ttttggtgac tcaagtgctc tgaaggttgg
     3421 tgagcctgcg attgcgattg gttccccact aggatctgaa tatgcaaatt ctgtcacttc
     3481 aggaatcatt tcttccttga accgacaagt gacaagtacg aatgaatcga acgaaacagt
     3541 caatatcaat gcgatccaaa cagatgcagc aatcaaccct ggaaattctg gtggtccttt
     3601 agttaatatc gaaggacaag ttatcggtat caactcaagt aagatcgcaa gtacttctga
     3661 atctacttct aatgtaagcg tagaaggaat gggatttgct attccaagta acgatgtagt
     3721 gaatatcatc aatcaactgg aaaaagacgg aaaagtgaca cgtccagctc taggtatcac
     3781 aatggtcgac ttatctgccg tttctactca gcaacaagaa caaatcttga agattccttc
     3841 atctgtaact aacggtgtca tcgtgacaaa tgtccaaaca gctacaccgg ctgaaaaagc
     3901 tggattgaaa caatacgacg tcattactaa gatcgatgac aaagaagttt cttctggtgt
     3961 agaacttcaa tctgctttgt ataagaaaaa agtgggagat tctgtcaaaa tcacttacta
     4021 tgaaggctcg aaagaaaaaa caactactgt tgaattgacg atcgatcaat cggcattaaa
     4081 acaaaatcaa aatagtgaaa attaataaaa aagaggttgt gacaaagact tgtcacaacc
     4141 tctttatttt ggaaatgctt ctccgactac ggcatcctca ggcaatacta agattccttt
     4201 ttttgctgga gcatttggca aatgcaattc acgagcggaa cagatcattc catagctatc
     4261 tacccctcgc aaagttcctg gccagatcat cattccatct ggcatcatag ctcctggttt
     4321 tgcaacgaca actttttgtc cagcttcgat attgggtgca ccgcaaacga tttgcaatac
     4381 tgtaccattg tctacttctg tctcagtaat cgataaatgg tcagaatcag gatgtgggac
     4441 gcatgttttt acgtacccga caacgatctt cggttcatga tcagcttcaa gctgttcttc
     4501 gaatcctgct tctttgatca atcgattcaa tttcgctaat tcctcatctg ttaagcttac
     4561 ttggccgttc ccttcgattg ttagatgatc ggagatatgg aaaaagttcc aagcaacaac
     4621 ttgaccttgt tcatctttga tccgagcaac attccatttg cgttcaacag tgttttctgt
     4681 tcctttatcg tcggctataa taaccatcaa ggtgtctccg acgtgtgctt tgttataagc
     4741 aaaaatcatg ttctttcctc cattatcctt tgctgagatt tatcttaaca aaaaattgca
     4801 ttcactgcat gaaatttttt tcatgcttta tttccgaaga agcctcttct ggaacaccat
     4861 ttattcgtaa caagggactg tgacaagact tttgtcacag tccctttact gttttagtta
     4921 attgattaaa aaggtcgtac aagttccact tgcttaatta cttggctgtt cttcttcgtc
     4981 agaaacgatc aagacatcac aaggtgaatg acgtatcacg tagctggcta cacttcctgt
     5041 cataaagcgt tcgactgcat ttagacctga ctgcccaacc atgattagat cgatctcata
     5101 tttttttggc aatttgactg ccatggattc acgaacagaa ccatatgcaa taattccttc
     5161 tatatttgtg taatcaacag atttcccata ttctttacat tcttcgatca tttccttggc
     5221 tgtttcagtt tgctgatcga ctagattttg atcaataggt gcatagccca taaagttata
     5281 aaattgctga tcgattacgt ttgctacata tactttccca ttattacggc gagccacttc
     5341 taccgctttt ttgaaagcag caagtgcttg ttcactaccg tcgataccta ctaaaatatt
     5401 cttatattct tgattcatct tgacccctcc taacagatgt cagccttttg ttacactttc
     5461 taggaacgat tcgatttcct gttttgtttt acgatcttta ttcactagac gacctaattc
     5521 ttttccgtct tggatcacga caaaacttgg gataccaaaa atgttccatt cagcggctac
     5581 atctatgtac ttgtcacggt ctacttcaat aaattgccaa tcagaaaagt cattttcaat
     5641 ttcaggcatt tgcggtttga taaaacggca gtctccgcac cagccggcag taaagaaaaa
     5701 tacactcttt ccagtttcta cgtagcctgc taattcttct aaactatttg ggataatcat
     5761 tctttgtcct ctactttctg tttatgtatt tttcaattaa attatacacc tttctttcga
     5821 actaacaaac attgatcctc tttttataaa aaaaatctgg caaaaagcaa tcttttgcca
     5881 gattttatag tttcactatc gcattatttt ccgtatacga tcgtatttac tgttgattta
     5941 tccaaacctt tcagtgtttg aatcaacatt tctcgtgcag cttcaaaatc gcgaatactg
     6001 aacatcgttt gatgcgtatg gatataacgt cccactacgc caatcactgt acttgggatt
     6061 ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc cttttgaaac aaagtattga
     6121 taagggatat tgtttgtttc agcgatatcc agcaagtact cgcgcaaacg aggaagcatg
     6181 atcaaacctg gatcttggat acgcatcaat gtaccttctc ctaaatgtcc gaatgtgcca
     6241 tttttcgtca ctgtatcatc tgctgcagag cagtctactg caaagaacag atctggcttg
     6301 aatttattga cagaagcttt agaaccacgc aagcctactt cttcctggac attcgcgcct
     6361 gcaatcaatg tatgacctaa tgtttcattt tgcaacgctt ctaaagcttc taataccatt
     6421 gtacagccat agcggttgtc ccatgattta ctgataatat tctttccgtt ggctgttttg
     6481 atcgtttcag cgtaaggaac gattgtgtct cctggaagga caccgtaact cattgcctct
     6541 tctcttgatt caaacccagc atcgaacaaa atatcggtta cttctactga tttttgtccg
     6601 cttgttcctc gcaataaatg tgggggaacg gaagaagaaa tacatggata attcttacca
     6661 gtagaggttt taagtgtaaa acgctgagca gaaacaacgt aaggattcca gccgcctaga
     6721 ggtacgactt taaaaagccc gttttcttgg atttgtgtca gcatgaatcc gacttcatcc
     6781 atatgtgctg cgaccattac acggggtgca tccgcttctt catgatggcg cagaccaaaa
     6841 atgccgccca aaccatctaa ctgtaactca tctaccagcg gttccatatg actttccata
     6901 taagcgcgaa catcttgttc aaaaccgctt gttccttgta attctgttaa ctctttgatt
     6961 cgttgaaatg ttttttcttc catatgctat tcgtctaaat ttcagactaa actcctctca
     7021 gacttatatt gatctgcgga taaaatatga ttttttctgc agaccgatcg tatttattat
     7081 agcttatttt ttctggtagc ggttatttat ttcaaagaaa atatgagagt gtggtaaaat
     7141 aagcacaagg agtaacaagt gagaagaagg aggtatgtcc acatgtatga agaaagcgaa
     7201 ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc taggattatt gagcggggta
     7261 gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag cagaccaagt acttgaaaat
     7321 atcaagtcat cttttctaaa agaaggtgca atcgaaggtt cttggatctc ttttgaaaag
     7381 gaacctatta ggaaattcgc cgttcggtcg agtgcttatc ggggaggtct ttctcgaata
     7441 gaagatggcg aaactgttta ttatgaattt ttagcagatg ctcatacagg aaccgtatta
     7501 gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca aaaaagtgag agaacgaata
     7561 cgcacccact attcaaaaga gaggtatcat gtccttcttt tgtagtgggt ggttttttag
     7621 atagagcttt ccaaacatat attccagtat tttcttttta aataaatgat atttagcggg
     7681 aaaaactagc atttcctttg attatgaggt atttttaatt tagcttttgt tttctatagt
     7741 taaggagagt gaaaaatgga acaacaagaa aaaattgctg ttctgattcc ttgttacaat
     7801 gaggaagcaa caatttcaac agtcattgca gactttaaac gagaattacc agaagcagat
     7861 atttatgtct atgataataa ttcgaccgat cgaacatacg aattggccgt tcaaggtgga
     7921 gcaatcgtaa aaaaagaacc tcgtcaagga aaaggaaatg tgatccgcca aatgttcttt
     7981 gatatcgatg ccgactatta tttaatggta gatggcgatg atacttatcc tgcagaagct
     8041 gtacatggtt tgctcgagaa attacgttct ggtgaagcag atatggtcat tggtgatcgc
     8101 ctatcaaatg gaacctactt tgatgagaat aagagacctt tccacgattt tggaaataac
     8161 cttgttagaa atacaattac tagaatgtat aaaacaaaaa ttcttgatgt catgaccggc
     8221 tatcgaggat tcaatcgtat ctttgtcaaa agttttccta ttatgagttc tggttttcaa
     8281 atcgaaacgg aattgacgat ccatgcttta gataaaaaat tcaagttcgt tgaaatccca
     8341 atcgattatc gtgatcgtcc tgaaggcagc gaatcaaaat tgaatacatt ctctgacgga
     8401 ttcaaagtga tcatgatgat tattaaaatg tttaaagatt ataagccttt gcttttcttt
     8461 gggatttgga ccatcttctt ctttttattt ggattggtca cggggattcc tgtgattcgc
     8521 gaatatatgc tgacaagctt tattacacgc atcccttctg cgattttaag tactggattg
     8581 atgactttag cacttctttc attagtaaca gggttgattt tggatacagt cgtcacgaac
     8641 gctaagaaag aatatgaatt gaatctatat catgtttatc atgaatacca cagtagaaag
     8701 taaaaaaagg atcaagactg caaaaataat ttgcaattct tgatcctctt tttaattaat
     8761 tatttcgcca aacgatagat ggcatctgca tagattgctg ttgctctgaa caagtcatca
     8821 agactcataa attcgtttgc ttgatgcatt gtatcagtat atcctgggaa catcgctcca
     8881 taagcaacgc cacgtttcaa caaacgtccg taagttccgc caccgatgat ttgttcgtag
     8941 cctttttctc cagtgtggtc ttcatagact tgcaataatg tagccaccaa tggatcatcc
     9001 attggtacgt aatgtggttc cattacgcga gcacctcgag taactgttgc gccttcttgc
     9061 cccactgttt gttcgatgtc actttgcaat ccgtctactg ttacaccttt tggaaaacgg
     9121 aaattcatat tgatgaagtt cgcttcctct gtaccatttt cttcaaaagc aaatagtcca
     9181 gcattcattg tcaatttgcc cattttttca tcttcataag cgactcctag tttttctcca
     9241 taaaaatctt catggacata agcagctgct acatggataa attgtttagc agatcctagg
     9301 aattcatagt tatctaagaa caatgccaag aaagaacctg cattgatacc agattgtggg
     9361 cttgcaccat gtgcgccttt accaactaat tcgattttta catacgtatt gtccgcttca
     9421 atcgttccgc ttactggatt tgcttcgatg aattggtaaa atgcttcttc catcgcgata
     9481 gctgcatctg cacttggtac ttgaagtgct gcagttgctg ttcctggcac catattttcg
     9541 cgaagtccgg aaacaaaaga tttcaagaca taatctcctg cattgtcgcc tttgaagcgt
     9601 aaagctaaag aaacatttcc tttttcacca ttgatgattg ggaattcagc atctggtgag
     9661 aaaccaaaat ctggttcttc ttcatgttcg aaataataag ccatatcgcc ccagccgctt
     9721 tcttcatcgc tgccgactac gaagcgaact ttttttgata gtttcaagtc aagttcttta
     9781 atgatcttca tcgcatagta agctgccata cttggacctt tgtcatcgct tgatccgcgt
     9841 gcaaagatct ttccatcctt gattactggt tcataaggat ctgtgtccca gccatctcct
     9901 gcaggaacga catccatgtg tccaaatatc cctagcgttt cgtctccttc gccaagatca
     9961 atatgccctg catagttatc gacatttttt actttaaatc catcacgttc gccataagct
    10021 aacatatgtt tcaatgcgtc gcgaggtcca ggtccaaatg gtgcatctgg tgttactttt
    10081 gagtcatcgc gctcgctgtt gacacgtaaa agatttttca agtcttcaag caagtcttct
    10141 ttacgtgctt ctacttcttt ttgccaatca attgtcataa agttaatcct ccacttattt
    10201 aagtcttcgt gttcatgata ccacaaatta cagaaaaaaa tagttcaaaa aatctgacaa
    10261 ttccttttct tatctgtttt tctctaaaaa aaatatacgc tataatgagt aaaaggaggt
    10321 ttttagatgc tgataaacga acaactagtg aaaagagtca tcacacctcg tccaaaaatt
    10381 tcacataaag gaacatttgg acgtatcgtt ttgattggcg gcaatgaaca atatggcgga
    10441 gcaatcatta tgagtgcttt agctgccgta cacagcggag cgggtctcac gactgtagta
    10501 actgatgaaa aaaatcggag tgctcttcac agtcatctgc cagaagcaat gattgtagtt
    10561 tggacggata ctgaccagtt tctttccgta ataaagcaag cagatgtact cttgattggt
    10621 cccggattgg gtacttcttc taagagtttg gaacgtttca aacaagtact agaaaaccaa
    10681 tcaaaagaac aatggctggt gattgatggc tctgctttaa gtttacttgc tgctaatcat
    10741 cttttgctac cctatcccga acaaactgta cttacacctc atcaaatgga gtggcaacgg
    10801 ttgagttctc taccgataca agaacaacgg gataaagaaa acaaaaagaa gcaacaggaa
    10861 atcggtgctg tgctcgtttt gaaaagtcat gagacaaaaa tttatggtca ccagcacttc
    10921 tacaccaatc ctttaggttc tgctgctatg gcaacgggcg gcatgggtga cactttagct
    10981 gggatgatca caggattttt agcccagttt cctcaaaatg accaaacgat tgcagcagct
    11041 atttatcttc atagtttcat tggagaccag ctggcaaaag aacaatatat tgttttgccg
    11101 acacaaatca gcgaagcact tccgaaatgg atgaattatt tttgtaaaaa atcaatggaa
    11161 aacgaggcat gagtcatgga gaaatttaca ggttatgtgc gagaaccttt ttattatgaa
    11221 acagatcaaa tggggattat tcatcacagc aattatatcc gctggtttga agaggctcgg
    11281 gtagccttat tggtacatct agggtttccc tataaaaaaa tcgaagaatc tgggatcatc
    11341 atccccgttc tggaagtaaa ttgcagctat aaagaaatga tccattacgg agaaaaagtg
    11401 agcatccatc caactatcca aaagtatacg gggacacggt tggattttag ctacgaaatt
    11461 gtgggaactg aagacaaaaa attaaaaact actggcagta gtaaacactg ttttttactt
    11521 tctgattcgc aacgattagt gaagctgagc aaagtcaatc cggagttaga tcagttattc
    11581 caagcgtacg cttctttgga tgatgtaaat tcttaataaa gaccgtctaa gcatttttct
    11641 tgtattgtgt cgagtagttc gtataatgaa aagaaaaaag caggtgatcc ccatgtatgt
    11701 cgttaaagta atgcacggtt acatcgataa aactggctgt cgcactcggg aaaaaaactt
    11761 agacaatctg ctcattttca aagacaaaaa agaatccgaa gcttttgcta aaagaatcgg
    11821 cggtcgtgtc aaaccgatcc aagaggtacg accagattga ttcaatattt ttcttttcga
    11881 ggctctaata ggcatgccta tcagggcttt ttttgcagac tgtgttttta tgcccacacg
    11941 atttgtaagc gatttatgtt ataatagatt atatctgcat agaaaggaag gttaaatggt
    12001 tatcaataat ttgcaacagc aagcttatga atctatacgt aaacaaatca tttattccga
    12061 gttagcacct ggaagtaaaa tctccgataa agtgttagaa gaacaactag ctatcgggcg
    12121 tacaccgatt caagaagcac tgatccaact gcgcaatcaa gagcttgtaa aaaccattcc
    12181 acagtccggc acttatgttt caaaaatcga tatccgatct gcttcattag cccgttatac
    12241 gcgggaaaag ttggaaaaac cgatcctgca agaatgcagt gcgaaaatga ccaagcaaga
    12301 tcaagctaaa ctagaaaaaa tactagaaca aaccgatcag gcagtaatcg ctcaaaataa
    12361 aaaagctttt tttgatttgg atactgattt tcaccgaact tgttatgaaa ttgctggaaa
    12421 acgagaaatt tgggattggc tagaaagtta tagtacgcac ttgaaccgtt tcagatggtt
    12481 acgtttgacg atttcagaat tagactgggg tcgagtatta gatgaacatc agacaatgct
    12541 tcaatcaatg attgatcata actttgatga agtcggattt ctttgcacga tgcatttaca
    12601 tatgattatc gaggagcaag aatacgtgat tcataattac cccgattatt ttgaagatat
    12661 tcaggacaga cccatcaaat aagaggtttt ggaatcagcg ttttgacctg agtattagag
    12721 aaagaaacgg caaaatagtt cctcatattt tgccgttttt tttgagctaa taccgagggt
    12781 cagctgattg cacaccgttt attgagaggt tgtgaaaaag ctgccctgca tcaagtaata
    12841 agaacagcca agtaaaaata gcttcttatg tttttaactt ggctggactt attaccgcag
    12901 atgcaagctt ttgaacaccg ttttccaaga ggtcgtgagc ctgccgttca tctccgagtc
    12961 acaaggaaca atttcaaaaa acagcttctc atgtttgttg aatattttga cttgtgaccg
    13021 aggagttgac aggagaacat cgtttattcg gaaaaaaagg actgtgacac gacttttgtc
    13081 acagtctctt tatttgtaat ctaaatcata ctccgtacat cttttttcta ttttttttat
    13141 ttcttctaag atcccttggc tacgaatttc attatatggc caaagtaaga ctgcctctaa
    13201 tacataaggc gaaaaatcgc tttgattcat ctcttttgaa aaactcatcg ctgctatctc
    13261 gctcaatgca cgaatgtttg attgatgtgt ataaaaaggc aaccgccaca gcacaatttt
    13321 ttcgctttgc gtataaattc ccttaacaga agcttcgatg tggtgaaata gttcatgtcc
    13381 aataatcaaa gcttgcgctg tttctttaga gatcgaagct agtcctgaac attttaattt
    13441 ttctaacggc tccgtcatca atcgaatccc ttcatcttct gcataagttg caaaagtgat
    13501 tcgttcgttt atttgctctg attcacagga aacgattgga acagccattt gttcacaaat
    13561 ttctcctaca tcggcaaaag ggtacttttt tttgatgcta catgccgttt cgataccaca
    13621 atcgattgat tgttgtataa gaattttctg cttggacaaa ggaatctttt tccaaaataa
    13681 atctcgccga aactggtaga atccccaaat ttcttcgggg atctgttgta attcgtttag
    13741 taactcttct aacttatgtt tgtttgatat ttccggcaac gacgatcact tcttcctccg
    13801 gttccatagt agatacttcc agatccatca aaagctgtag ttgttccaga tccgtcgctg
    13861 taaaatcaga gactcttgct cctaagcaga gataaaattc tggtcgggca atcaattcag
    13921 tttgataaac tgccatatct tcccaaagtt gttcgacagc tgtcatataa ttggctgcag
    13981 ttgttttcat acatgaagca tgtccacgtt ggaccttgat aaatcctttt tgatcgatga
    14041 tccgaagact tccagcattt tttgaacgat tctgttcgcc gcatacataa aagaaaggtg
    14101 tattttccaa taagcaaact tcatttttat ttaatcgcat atcctctgca gcaagctcta
    14161 gtgcttcttc tgttgtgatc tccttggtta gatcggttgc tttcacttcc gtagaaccag
    14221 tagcgatagc cgttactttt gaagtctgtg gatcgatttc tacgtggact tcgatcgatt
    14281 ctggtgtagc gcctgattca atcgctttat tcatcgcttc atttttcaac gatcgtatat
    14341 cttctttact tggcgaaggg atgatacgtt ctacaacatc tcggaccatt gcaagtgcta
    14401 caccaataga agaaatcact tcggcgtttt caggaatcga atatttcaca cccattttat
    14461 tactaaagta agtaatcaat gaggcagcgc cgccccctac acctacaaga gaaatctgat
    14521 ctttctccaa gtgatacttg tctgccaatt ctaaaatcac cggttcaatc ttagcatagg
    14581 atttttccat gatctgttct gcgatgtctt caaccgtcgt atgacaataa tcagctaatg
    14641 cttggatggc ttttcgtgca gatgggacat ttccataaga aaaatgttct tcttgcacta
    14701 atccaaggac attggctgca catgtatttg taatagtcac ttcttctccg tcttccatca
    14761 ctaccttgac gtaatcagca gggtctcctt ctttaggaga gaaaaattca accttaggac
    14821 ctttgatttt ttctgtctcc gtaaatactg cataatctag accggcaata tgtgcagaac
    14881 gtggaccgac atcgatgatt cctgactgat tagcccgtac catcgaaccg cctgccacac
    14941 ctagaacccg aacatctaat gaagagatat atgttgggtg gccacctaca attgaataat
    15001 cgattgctgg acgtccattt ttaatgaccc cgatattggt tgttgttccg cctacttcaa
    15061 aataaacacc attagacgct ctcaagtaca tcaaagagcc catgactgat gctgctggac
    15121 cggaaagcat ggttaaaaca ggtcgttttt tcatttcatt gatttccatt actccgccgt
    15181 ctccgcgcat aatcatcaaa gaaacattga cccctgcttc tcgtacggaa tcttctgtag
    15241 aggttgccgt atctagcatt tttggtaaaa tagatgcatt gattgctgct gtccgagttc
    15301 ttcttgtcaa tccatataat tttgtgatgt cagaagccat cgttgtaggg atcgatttta
    15361 ccgatgctgc ttcataaaca actcgttctg gttccccatt atctactcca aatgccatag
    15421 aagaaaccaa gacctccgct ctttctcttt ctaaagaaga aatcgtttct gaaacacggt
    15481 ctacatttaa gtgtttgaca ggtaagaatg cattgacaat ctctattttt ttcttattcc
    15541 ctaaatcgat atcgttcaat ctagtttgtc gttttgctaa aaaaccttct aatccacctt
    15601 ttgccatccc gattacgccg acttttgcta cgtctccttc aatcagtgca ttagtcgctt
    15661 gtgtggtaga atgtgcaaca aacacaacat cttctggact gatattattt tccagcaaac
    15721 aattttgaaa agactgaacg acacctgcgg ctactccacg gacatcatcg tgggttgttt
    15781 tcactgaaga ttttccgatg atttcgtgcg tcgcattatc gattgcgact gctttcgtat
    15841 gtgtaccgcc aacatcaatt cccattcgca caaaacgttt cattttcttt tcctccattc
    15901 atctaatcgt ttttatcgac gttttgttta ctaggcgttc ccgtacaata agaacgtgat
    15961 caggcataag atcgctccga tgatccaacc agttgggatg gatttcttca tatattctcg
    16021 agaagttact tttgtataac ctaatcccca agcaacccaa gattgtgtaa tatctaaatg
    16081 ctgaggagca atcgttgtga ttgcaaataa cgggaacaaa aaggctggcg acatcgttgg
    16141 attaacagct aatacgacag ctaaaatagc tgagcctgaa ccgactagat tcattggacc
    16201 gcggaaaaat cctagtggtg tcaatatagc aaatacgatt gccaagacta atggtgattg
    16261 cggtatccaa tctccaaaaa tagctgaaaa ataaggagaa gcatacgcag ctgaattatt
    16321 gaacatcgct aaggtcatca agaaaccaac cattggcgca acatctactg cgccatctgt
    16381 aaataatttc gcaaactgac gacagatttc tttataacct ccgcgcaatt ttccagtaaa
    16441 cagcaatgcc cagatacctg ctagaataaa acctaagatg attggaagat ctaaaagaac
    16501 gacccctaaa actggtaata atacggctaa ccatgagatc attggtgcgt cgccaccttc
    16561 agctggtacg tttgctgcca ttgcatagcg tttctttttg ttcattgata tattagcgac
    16621 cgttagtacc acaactaagc ttacaatcat cccaatcata ccaatttgga aatagtcatt
    16681 ataagtatag ctttcagccg ctggattgaa accagcataa atcgtttgat attgtttgaa
    16741 attgacgata ttagcaaaga ttccagccat aatcgagccc ataaatgaga acagtgcaac
    16801 cggtactgaa atacctaatg acatcaagat tggcaacaca ataactgcaa tagaaatcac
    16861 tggtccgatt cctgtcatcg acatgaatat aacagcggtt acaatacata gtaagctcat
    16921 cgtgattctt ggcttgtccc ctccaagttc aactaccttt cgaatcaggg tagctgcgat
    16981 tccgctatct actagaacac gcccgaaaaa ggcaccaaag aagacattta ccaagatcga
    17041 tttggcgtac tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa ttactccttg
    17101 cccttccatc gccgggtttg gtgaaaacgt gtttccgact agcgctaatc ccatccatac
    17161 agtagccatg atggcaaagc cgaccatcaa gttgtagcct tttacacagt accagaccat
    17221 gaaaaagaat gagataacta acaaaatacc aataataata tcactcatgt tattcctccg
    17281 tccattttgt ttttctgata agcaaactaa tatatcagtc aaactcattc taacttatgt
    17341 agttagcgct ttcattaaag ggtgaccata aatgttcagt tttcaattaa aaaaccttaa
    17401 ttatgctatt ttagacttat acgttattgt cctgtttcat ttgatggagg attgtaaata
    17461 aaaaaagagg aaagaacata acttttcgtt atcttctttc ctctttataa ttgtttagct
    17521 tatttagcca aagcttcttt tgcttggtca gccaatgcag taaatgctgc tgcatcgtta
    17581 acagccaagt cagctaacat tttacggttg atgtcgattt cagcaacttt caaaccgtgc
    17641 attaattttg agtagctcaa gccattcata cgagctgctg cgttgatacg agcaatccac
    17701 aatttacgga agtcgcgttt cttttgacga cgatcgcgat atgcgtagtt gtatgaattc
    17761 attacttgtt cttttgctgt tttaaataaa gtgtgttttg aaccgtagta accttttgct
    17821 aattttagca ctcttttacg acgtttacga gttactgttc cacctttaac acgtgccatg
    17881 tataattcct cctaataaaa ataaatcttc agattctgtt atttgtactt cttatttcat
    17941 ttttgctagt tgttggcgaa tacgtttgaa gtcgccacta gaaaccatgc ttgctttacg
    18001 caattgacgg cgttgttttt ttgttttgcc gtggaaacgg tgacttgtaa acgcgcggaa
    18061 gcgtttcaat ccgcctttac cagtacgttt gaaacgtttt gctgatccgc ggtgtgtttt
    18121 ttgttttggc atgactatat ttcctcctca aaatctttcg ttatccgact atcggctgtc
    18181 caacagccag ttagtcattc aaattactta ctgtcgtttt tcggtgccag cgtcaagaac
    18241 atgctgcgtc cgtccatttt cgctttttgt tccactgttg caatatccgc agtttcttca
    18301 gctaagcgat tcaagacttt ttgaccaatt tctttatggg taatggcacg gcctttgaaa
    18361 cggatagaag ctttcacttt atcgcccttt tccaagaact tacgtgcatt acgaagtttt
    18421 gtattgaagt cattcaagtc gatcgtagga cttaagcgaa cttcttttac attgatcact
    18481 ttttgttttt tacgcgcttc gcggtctttc ttttgttgtt cgaaacggta tttaccgtag
    18541 tccatgattc gcgcaactgg tggtttcgca ccaggggcaa ctaacacaag atccaagttt
    18601 gcttgttctg caatattcaa tgcttctgct tttgtcttaa ctcctaattg ctcaccatct
    18661 gaaccgatca atcgtaactc tcgtgcacga atgccgtcgt taaccatcat atcctttgct
    18721 atggtcattc acctccaaat attatgagag aaagaaaagc ttaccgaata aacggcaata
    18781 aaaaaacgag ttctcttaca gaacccgcgc gtacttcacc taatagaacc agtgaaaact
    18841 atctagccca gtgacattat caactaaggc gagaagcggg tgcttctgct tttatttctc
    18901 aaccatcata gtatagcaaa aactaatctt tttgtcaatg tttttcatac agtttgtctt
    18961 ccttttctga aaatgattag attagcagac tttcttgcag tgattcccgc ttctttgcta
    19021 tactaaagaa aaacgaggtg aacccacatg aaaaaaatcg aacctattcc accttctctt
    19081 cctcttctta caaagggaga gttttattta gaagatgagg agatcttctc tggtcttctg
    19141 atcaacgggg gtgaccaaag ctatctcgat gccaaaaata tggtattaag agagagtcat
    19201 atccaaaaac ttacgatgca aaaaacagtt ttacgtcatt ttgaatgcag taatgtcatt
    19261 tttgaaaaat gtgatttttc caatctggag tggattggtg gcagtttcca ccaagtcgtg
    19321 tttcatcaat gtaaattaac aggtacgaat tttgccgaaa gttatcttcg agactgtact
    19381 ttcactgatt gtattgcttc gatggcttcc tttagtagta caaacttgaa agctgtctcc
    19441 ttcgatcatt gtcaattaga agacagcgaa ttttatgagg tcacttggaa aaaccttttt
    19501 ttgtctgaca atcagttgac cggttcgaac tggtttcgaa cttctctgaa gggattagac
    19561 tttacgacta atttcttcac agcaatcgct ttatctcaag attctttggc gggactaatc
    19621 gtcaaccaag agcaagcact tgtgatcgcc caagcccttg gtttgcagat caaagactag
    19681 atcaacgaat ccgccgccac agtgcaataa gcatccaaat agataagacc aaactgatga
    19741 tcaaggcgat tccccatccg aaaacggaat gggtaaaggg taactggaga ttcattccga
    19801 agaatcctgt cacaatagtt ggaacggtca agatcagcga ccagaccgtc aaaaatttca
    19861 tcgtgtcatt caagttgtta ttcagcaaat tattataagt gccagagagc tggtctaaaa
    19921 tctgcgaagc taagttcgtc atttcaactg cttgtcgtgc ttcgatcagc gcatcgtcca
    19981 actcttcttt ttccttttct gacaacttgc gataaatagc caatgccttg atttgttcta
    20041 aaagtactac attttgtttt gttcctgtaa ctaagtaaac caaaccgatc tctaaatctg
    20101 aaagttgcag cagattcttg ttcgttgttt tttctcttaa tttcagatta agccgctgac
    20161 gttcactatt cacttcctcg attaaaggaa aaaaggaatc agaaatcaaa aacaatgtac
    20221 gaaacaataa actgtataca ctcatgtctg gcattcgttc caaaagcgat cgaattagtc
    20281 gaatcacgta ctcggtattc ggcgtggtaa atgtaaacaa accgtcttct tttaaaataa
    20341 aagcgatagg actagtttca taatgattat cgactttttg ctggtgcgga acatttgata
    20401 caagcagtaa cgcttcatca aaagaatcgt actctacgcg cgcccgttca tttttatcta
    20461 gagaataagt cagcatctcg tcactaatgc catattcttc ttttaagttt ttaacagcta
    20521 gcacatcgtt ttgctgtaca ttgatccaaa aattacggtc ttgattaaaa tagtgttttt
    20581 cccgcatact ctatcctcct gacatttgtt tccagtataa agaaaaaagt cgctcaaata
    20641 aaggtttacc ctttatttga gcaactgtaa atcaaatggc tactttggtt tggcgaatct
    20701 ctgctaattt ttcttcgatc attttcaaga cttcttcttt tgcttgagga tcttccacaa
    20761 aattatagcg gtctccatcg atttggattt ttggactttc tttgtattct tcataccatt
    20821 gatcgtaacg tctgttcaat tctttgtaat aatcgtatag acttggatca aaggacaatt
    20881 gttcatacga acgaccacgt ttttcgatac gctccaacat cgtatcaaaa gatacacgaa
    20941 tatgaactaa taaatccgga tgttttttat gggcagcata gggaagttct tccatcatgt
    21001 tttccagcag ttcatcatac acacgaactt ctgtttctgt tgctcttcct agatctgcat
    21061 ttagatggaa aagcagtgaa tcttcataaa tcgaacggtc caatacatta ttgtcatctt
    21121 tcattgcttg tttgatgctt gcaaaacgtt tattcaaaaa ataaatttgc agcaaaaaag
    21181 catactgttc agggtttgca taaaataagg gcaagacttc attatcatca atcgattcat
    21241 aaaatgcctg actatcaaag tgttcagcca tcatttctgt taaacttgat tttccggcac
    21301 caattgtacc tgctaaaacg atcacgctca tttacctcca gctcacttca tcactaaata
    21361 ttgtgtttaa ctccaacctt tttaaatata cccccaatat ctagtagtgt caattgtatt
    21421 tttttctagt caaagaagaa aaaaagtcaa aaaattctta agaaaaaagt gttttttctt
    21481 tagaaaacgt attcttagtt ttacttctga gatggtttta gtaaaataaa ggaggaatgt
    21541 tttttgagac ccttagtgga caaaaaaatt cgtgaagcag cgcaactcgt tttgggcgct
    21601 ttattttttg cttaaaaatc acttttacgt gagaaggagt tagattatgg caaactatgg
    21661 ccaaggagaa tcaagcggaa agatcatatt gatgggcgag cacgcggttg tttatggaga
    21721 accagcgatt gcctttcctt tctatgcaac aaaagtcacc gcattccttg aagagctgga
    21781 tgcaatggac gatcaactgg tttcttccta ctattcagga aatttagccg aagctcctca
    21841 tgcattaaaa aatatcaaaa aattattcat tcacttaaaa aaacagcatg acatccaaaa
    21901 aaacttgcaa ctgaccattg aaagcacgat tcctgctgaa cgtggaatgg gatcaagcgc
    21961 tgcagtcgcc acagcagtca ctcgtgcttt ttatgattac ttagcatttc ctttgtctcg
    22021 tgaaatacta ttagaaaatg tccagctttc ggaaaaaatc gcccatggta atcctagtgg
    22081 aatcgatgca gccgctacta gcagcttgca gccgatttat tttacaaaag ggcatccttt
    22141 cgactacttt tctttgaaca tcgatgcttt tttgattgtc gctgatacag gaatcaaagg
    22201 acaaacaaga gaagccgtca aagatgtcgc tcacctcttt gaaacccagc ctcatgaaac
    22261 tggacaaatg atccaaaaat taggatactt gacgaagcaa gcaaaacaag caatcattga
    22321 aaattcacca gaaacgttgg cacagacaat ggatgaatca caatcacttc tggaaaagct
    22381 gacaatcagc aatgattttc ttaatctatt gatccaaaca gcaaaagata ccggggcatt
    22441 gggtgccaaa ttaactggcg gtggacgcgg tggctgtatg attgcattag cacaaacaaa
    22501 aacaaaagcc caagaaatca gtcaagcact tgaagatgcg ggtgctgctg aaacttggat
    22561 acaaggatta ggagtacata cctatgttta aaggcaaagc acgcgcatat accaacattg
    22621 ctctaatcaa atattggggt aagaaaaatg aagaactcat ccttccaatg aacaacagcc
    22681 tttcgttaac attggatgca ttttatacag agacagaagt catcttttct gatagctata
    22741 tggtagatga attttatcta gatggcacct tgcaagacga aaaagcaaca aaaaaagtca
    22801 gtcagtttct tgaccttttt cgtaaagaag ccggcctctc actaaaagct tcagtaatca
    22861 gtcagaattt cgttcctacc gcagctggat tagcctcctc tgccagcggg ctagctgctt
    22921 tagcaggcgc ttgcaatact gctcttaagc ttggattaga tgatctctct ctttcaagat
    22981 ttgctcgacg cgggtctggt tcagcttgcc gaagtatttt cggtggtttc gtcgaatggg
    23041 aaaaaggcca tgacgactta agttcttacg ctaagccagt cccttccgat tctttcgaag
    23101 acgatttagc aatggttttc gttttgatca acgaccagaa aaaagaagtg tccagcagaa
    23161 atgggatgcg tcggacagtc gaaacatcca gtttttatca aggctggtta gattccgttg
    23221 aaggggatct atatcaattg aaacaagcaa tcaaaacaaa agatttccaa cttctcggag
    23281 aaacgatgga aagaaacgga ctaaaaatgc atggaacgac tttagctgcc cagcctccat
    23341 tcacttactg gtctcctgac agcttaaaag cgatggatgc ggtgcgacaa ttgagaaagc
    23401 aaggcattcc atgctacttt acgatggatg ctggccctaa tgtcaaagtt ttagtggaaa
    23461 acagccattt atctgaggta caagaaacat ttaccaaatt atttagcaaa gaacaagtaa
    23521 ttactgcaca tgctggtcca ggaattgcga ttattgaata aagggagatt tttatgattg
    23581 aagtatctgc accaggcaaa ctctatattg ccggagaata tgcagttgtt gaaacaggcc
    23641 atccagcagt tatcgctgca gtcgatcaat tcgtgacagt cactgtagaa tctgcacgaa
    23701 aagtcggaag tatccaatct gcacaatata gtgggatgcc tgtacgttgg acaagacgca
    23761 acggagaatt agttttagat attcgggaaa atccttttca ttatatcctt gctgctattc
    23821 gcttgactga aaagtatgca caagaaaaaa acattctttt atccttttat gatctgaaag
    23881 taacaagtga gttagacagt tcaaatggcc ggaaatatgg attgggttct agcggtgccg
    23941 tgaccgttgc aacggtcaaa gccttgaatg ttttttacgc gttgaattta tctcagttgg
    24001 agattttcaa gattgcagca ctagccaatt tagcagttca agataatggt tcctgcggcg
    24061 acatcgctgc tagctgttat ggtggctgga tcgctttctc aaccttcgac catccttggc
    24121 tccaagaaca agaaactcag cattctatca gtgagttact tgccctggat tggccaggtc
    24181 tatccattga gccattgatt gctcctgaag atttacgttt attgattggt tggacgggta
    24241 gccctgcctc tacttctgat ttggtcgatc aagttcaccg ttcgagagaa gataaaatgg
    24301 tggcttatca gcttttctta aaaaatagta cagaatgtgt caacgaaatg atcaaagggt
    24361 ttaaagaaaa taatgtaacg ttgatccaac agatgattcg aaaaaaccga caattactgc
    24421 atgatttatc tgcaatcact ggggtcgtca tcgaaacgcc tgctttgaac aaattgtgta
    24481 atttagctga acagtatgaa ggagccgcaa aatcttctgg tgcaggtggg ggcgattgcg
    24541 gaatcgtaat tgttgaccag aaatctggca ttcttccttt aatgagtgca tgggaaaaag
    24601 cagaaatcac tccactgccg ttacatgtct atagcgatca aagaaaggaa aaccgatgaa
    24661 tcgaaaagac gaacatgttt cactagctaa agcctttcat gataaacaaa aaaacgaatt
    24721 tgacttcgta cggatcatcc ataacccttt gccgcaaata gcagtttctg atgtcgatct
    24781 aagtacacag gcagttgggt ttacactaag cagcccattt tatatcaatg cgatgactgg
    24841 cggaagcgaa aaaacaaaaa aaatcaacca agatcttgca atcgtcgcaa gagaagcaga
    24901 tttgatgatt gccactggtt cagtcagcgc tgccttgaaa gtcccttctt tagccgatac
    24961 ttatacgatt atgcgacaag aatatcctca cgggaaaatc attgccaaca tcggtgcagg
    25021 gacttctgtc gaaagagcca aagaagctat ccgattgttt cacgcagatg ccttacagat
    25081 tcatttgaat gcacctcaag agttggttat gcctgaggga gaccgtgatt ttaccaattg
    25141 gaaagtactt attcaagaaa ctcaaacagc cattgacgtc cccctcatcg taaaagaagt
    25201 cggttttggc atgacaagag aaacgctcaa tgatctagct gctttaggag ttcatacagt
    25261 tgatatcagc ggtcgaagcg gaacaagctt cacccagatc gaaaacgccc gtcgctccaa
    25321 gcgggaactg agttatttag ctgactgggg gcaatcgaca gtctcttcat tgcttgaagc
    25381 aaatgaagca gacacttcta tggaaatcct agcctccgga ggtatccgca atgcctatga
    25441 catatttaaa gccctttgtc tcggcgcaaa tgccgtgggt acttcaggaa ccgtactgac
    25501 gcatctgatg aatcacggcg tagaagaaac tattatactg atgaagcaat ggcaagaaga
    25561 gcttcgtctg ctttatacta tggtcggcgc aacgaatacc gcggccctac accaacaatc
    25621 tctgatcttt tccggaccag tgaaggattg gtgcgaagca agagggatcg acttagtgaa
    25681 gtatggaaga cgcacagaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
    25741 tctaaacacg tacaaacggc ggaagtaaaa gcaactcctt cggaaataag ctaaaattca
    25801 caaaaatttg aaaagcaatt ttcgtgaatt ccttcttatt tcttggagtt aaatgcttct
    25861 gtcccagcct cttttttcga ataaatgatg tttaatcagc tgtccttcgg tattcgttca
    25921 atccgctgat ttcacaacct ctccactaaa cagtgtacta aagctctttt aaggttgttt
    25981 tccgatatag gcaagtatgc ctccgtccac atacaagaca tggccgttaa cgaaatcaga
    26041 tgcgctagaa gccaagaaga cagatggtcc agccaaatcg attggctcgc cccagcgtgc
    26101 tgctggcgta cgtccaacga taaattgatc gaatggatga cgttcaccgt tttcttgtgt
    26161 ttcgcgtaat ggagcagttt gcggtgtagc aatataacct ggtccaattc cattacattg
    26221 gatattgtat tgaccgtatt cagaagcgat gtttttcgtc agcattttta atccgccttt
    26281 tgccgcagca taagcactga ccgtttcgcg tcctaattca ctcatcatgg agcagatatt
    26341 gatgatttta ccgccgcctt tttcgatcat atccggaata actgcttttg aaacgatgaa
    26401 tggggcattt aaatccacgt cgattacttg gcggaagtct gccgcatcca tttcgatcat
    26461 cggtgtccgt ttgatgatcc ctgcattgtt gactagaata tcaactgaac ctacatcttc
    26521 tttgatctgt tgaaccatcg cttgaaccgc ttcttcatcg gtcacatcgc agacataacc
    26581 ttttgcatcg atgcctgctt ctttataatg cgctatacct tcatcaactg attcttgatt
    26641 caagttatta aatacgattt ttgctcctgc tgtagctaaa gagcgtgcaa tctcaaatcc
    26701 aataccatag acagcaccgg tgactaatgc tacttttcca tctaaacgaa acatatccat
    26761 actaaattcc atcattttct ctcccttatg cttattttaa ttgatccatt tgcaccatat
    26821 ccatgtctgt ataggtgata ttttctccgc acattgccca gatgaatgag taattgcttg
    26881 tacctacacc tgaatggatc gaccagcttg gcgaaataat ggcttgttca ttgtctacaa
    26941 ctaaatgttt tgtttcatct ggtttgccca tcatgtggaa cacacgtgta tcttcattgg
    27001 cataatcaaa gtagacataa gcttccatac gacgttcatg tgtatgacaa ggcatcgtat
    27061 tccaagcgct tcctggttct aaaatcgtat aacccatttg taattgacag ctttcgcata
    27121 catttggatg gatgtattgg tagattttac gttcattcag tgtcaggcct tcaccagtct
    27181 ccattggttt tacttggtcg atactgattt ttacatttgg atatttatga tgagctggaa
    27241 cagaactaat atagaattta gctgggtttt cagcatcttg agaagaaaat acaacatgtc
    27301 gtgtttcttt tccgatatag tatccgtctt gttttttcat ttcttctttt tgcccatcga
    27361 tctcgatgaa tcctggtcca ccgatattga tcacacctag ttcacgacgc tctaagaagt
    27421 aatcaacacc tagttctttg tctaatgtga tttctaattc tttttcagta ggtgtcactc
    27481 caccaaagat cattcgatcg ttgtgcgtat aagtcaaact gattttccct ggttcaaata
    27541 ccttttctac taaaaattct tttctcaatt gttccgttga ataatgacgg atatcttctg
    27601 gactgtgtgt ataacgtgtt tccatttctt gcattttctt ctactccttc tatcgaatga
    27661 tttttccaga gccgcatgct aaaaattgtg cgacttcttc ttgactaaat tggttgcagt
    27721 ccccatggat cgtatgcttc aaggcagaag ctgccgtagc aaaatcaatg atttcttgcg
    27781 gagacatatt ggataaaatc ccatggagca cgccacctgc aaaagcatct ccgccaccta
    27841 ctcgatccac gattggggtg attctatgga cttgggattc cacatattca tggttcatcc
    27901 acaatgtccc cttcaattca ttttcacttg ctgaatgaac ggtacgcttg gtcgaataaa
    27961 acacctgaat attcgggaac ttttcctgca ttttttgata ataataaatt agaggttgtt
    28021 cttctgcttc tggcgctttc tcgataccta acaaataaag agcatccatt tttccagctg
    28081 agcagatatc tactaaaggt aaaagctgat ggattacttc gctggcttct tgctgtgtcc
    28141 ataattttcc acgatagttg atatcgaagc tgatcaggca gccagctttt tttgcagctt
    28201 ctatcacctg tttcgtcatt ttttgccatt ttttagacaa agctggggtg attcctgaaa
    28261 catgaaatat atctattcct tggaataact cctctagcga ccattccaaa tgatccattt
    28321 cagcaaaact tgatccggca cgatcataga tcactgaggc ggcgcgttcg ccactaccag
    28381 tttccatata ataagtacct agtcttgatc caccagataa tacctgtttc gtatctactc
    28441 cataacattg caagtgcttt ttgactgctt ttcccaatgc gttatcaggt acttttgtag
    28501 caaaaatcgc ttcatgtccg taattagcta aagaaattgc tacgtttgct tcccctccgc
    28561 cataatgggc agcgaatcgt tcacttgaat cgatacgggt tccctgctct gtggacaatc
    28621 gcaacatgat ttctccgagt gtcactactt ttcccataag cagctacctc ttgatttctt
    28681 tcatttttgc tgcatactgt tgcgcgactt ccgttacttt gtcaaaatct cctgtagctg
    28741 cgggtgctaa aaggttccca cctactccga ctgttacgac accagcatcg aaccactctt
    28801 tcatattgtc cagactcaca ccgccagttg gcatgatatt gacttgaggc atcggcgcct
    28861 tgaaggcaga aatgatactt ggtccataag cgcttccagg aaatagtttg acgatatcta
    28921 ccccgctttt cagcgctgtt ttgatctcgg taatagtcat acatcctggt aagtaaggaa
    28981 tttgatacag attgcacatc tctgccgttt cttgatcaaa ggaagggcta acaatatact
    29041 ctgcgccagc taagatagcc aagcgagctg tgacagcgtc taatactgtg cctgcgccaa
    29101 tcactatttc tggttggtct ttgtagaatg acaaaagttc tttgattact tgatctgctt
    29161 gagggactgt aaaagtcaac tcaataccag ttagaccacc tttgatgatt gcgtggcaag
    29221 cattcaaagc ttcttctttg ctttttccgc gtaccaccgc aatcactccg gcgtttttca
    29281 atcgagataa aatatcaatt tttttcatct tctccaccta tttcctattt agaaatttta
    29341 ttttataact tggaaatcta tacccatatt atacggcaga aaagaaaaat tgcaaacgct
    29401 tttttcatta tttttgaatt ctttgctcga tttcgctttt tgtttgcttg agggcactga
    29461 ttatttgttc ttttgtctca tctgttaaac gatatttcgg catacttaca ctaaatgcac
    29521 caatgatttg ttcgtttttc acgattgagg ctcctataca aaaaatatca cgttcgattt
    29581 cttcatcgtc aaatgcgatg tgggtttctt ttaccgtttt gatttccttt ttcaaccgta
    29641 atgcattggt gatcgtatat tctgtgtaag ggatcaacgg atgcttttca agataatcag
    29701 tgtactgctc ttcactgaat tctgctaata cggctttgcc catcgcagaa ttgtataatg
    29761 gtcgggtgat tccgattttt gaagacatgc ggatcgtttg atgttttgga tctagtttgt
    29821 tcacgtacaa gatctcattg ttgtccaata cccctaagtg gatagtctca tcgatctttt
    29881 gctgcagctg ttctaaaaac ggtaaagtca actctaccaa atcgatttgt tcaatgtttt
    29941 tattcgcgta gcgaataagc tttgcaccta aacgatagtt tttctctgaa tttttattta
    30001 cgtagccgat catcaacaaa gtatctagta ttttcaagac tgtagaagag gtcattccca
    30061 caccctttgc gatcgtctgc aaagagacat cttggttttc cgctaaatag tctagtattt
    30121 tagaggcttt gatcaataca gttccataag gctttttatt ttccatataa tattctccta
    30181 tttctttata agaaaaatta tagcggaaat accatcaatt taaaagagat atccagatat
    30241 aaactaccat ttctctttac aggcaattta atgttgaaga tccgcttcgt ttttacatca
    30301 aactttgata taaagcaatg atttcttcta ctgtcgtttc tcttggattt cctggtgtgc
    30361 atacatctcg caaagcatct tctgcaattg ctggaatatc tgcttctttt acacctaatt
    30421 cagaaatggt ttctgggatt ccaaccgctt tgctcaaccg atcgatctca ccacaagccg
    30481 cttctcttac atcttctaaa gacatttcag ctgctccttt aatccctaga acaagagcga
    30541 tctcacggta cttttctcca gtatattctt tgttatattt catgacagtt ggcaaaagtg
    30601 ctgcacacgc tacgccatga ggaatgttgt accatgcact taacgggtga gccatcccgt
    30661 gtaccagtcc taaaccgaca tttgagaagc ccattcccgc aatatactgt cctaatgcca
    30721 ttttttctcg cccattttga tcgccatcta cagaagcttc caaagaatgg ccgatgattt
    30781 cgattgcttt taaatgcagc atatcagtca tttcccaagc gccttttgtg ataaaccctt
    30841 caatagcatg cgtcatagca tccatacctg ttgatgcagc caattttttc ggcattccca
    30901 tcatcatatc gctgtcgatg aacgcgacga ttgggatatc atgaggatca acacaaacaa
    30961 attttcggtg atttacttca tctgtgatga cgtagttgat cgttacttct gctgctgttc
    31021 cagaagtcgt tggtatagct aatataggta aacatggatt tttcgtatca gcaacacctt
    31081 ctaaactaat gacgtctgaa aactctggat tcgtcaaaat aatcccaatg gcttttgccg
    31141 tatcgattgg cgaacctcca ccgattgcga tcaaacagtc tgcctctgcc tctttaacaa
    31201 aagccactcc atcttttaca ttttggatcg ttggattagg tacgatcccg tcgtataaag
    31261 cataagcgat tgctgcttta tctagaagtt ctgttacttt tgttgctata ccgtgctcga
    31321 tcaatccttt gtcagtaatg acagctgctt tgccgaatcc tcgctgctga aattcaggga
    31381 cgatcgactc aatcgctcct tttccaaaat acgatgtttc atttaaaatc attcgattac
    31441 tcattttcta ttcctcctaa tttgtattat ttttcacaat aaaaacgaaa gaaccccaag
    31501 caaccggttg cccagttgct tgttgctaca gttcaaagac tggcatcaat tcccaactga
    31561 ctggagaggc atctggattt gtctccatga tatcttccat gtacttccac catttttgat
    31621 tgatagatgt atttgccatt ttttcccata acttttcatc ttcgatcact aagtaaccaa
    31681 ataacaaact tgtactcgga tcaagataga tcgaatagtc gatgaccccg tgttcacgaa
    31741 gtgctgtacg catttctggc caaagttcat catgtctttt tttgtattct tcttgttttt
    31801 ctgggtaaac tttcatgcaa aaagcttttt tcaccatgtc agacacctgc tttcgtatga
    31861 acttcctcca aataacctgc ttttggagtg acaccaaacg catctgctaa atgccacagg
    31921 ttttcgtccg tgattgtttg caagattggt ccttgcgctt tgacgtacgt atagacttct
    31981 gctgcctttt ctgctgtctc gatcaaacca aagacttcat ccatgtcttt tcctgacccg
    32041 tagattccat gttgtggcca taaaaccaat cgagtttctt tcattttttc agctgttgct
    32101 tccccaatct catttgttcc cggaaccatc cacggaatga tggaaattcc ttctggaaaa
    32161 acaactaagc attccgtaca catttgccat agcgtacgac taaaggaacg ttcgtctaat
    32221 tcatgcgtaa aactcatcgc taatagatga ctagcatggc aatgcataac gatccgatta
    32281 tctgggtcta cttctaaacg ggcgatatga ctcatgaaat gacttggcaa ctcgcttgta
    32341 ggaaggcatt cgttttccag cccccacaaa agttccagtg ttttgccatc ttcagcaact
    32401 cgaacgattc caagattctc ggcaggttcg ctcgctacat ttttaaaata tcttcctgaa
    32461 ccagttacaa taaaatagca tcctgctaaa cggctggcat caaaaatcat cgagatgcgg
    32521 cggatcactt tttcaggatc caagaacggt gtgatctctt cttcttttag taaataagaa
    32581 atattcccgc catttctttc atcccatccc aagcgataaa gattcgacgt cgtttcgatc
    32641 atttctttca cataaggcgc ttgtaaaata tctatttttt tcaatatagc cacactccta
    32701 ttttcttaat tccaacacgt ctttttgata ttcctggact tcttccagcc aatccaaacc
    32761 aactggaact tgatttttgt aacagaaata attccataca tcggcatacg ggaaatcttt
    32821 caattcttcc gtataagcca ttcttgaagt aaagtcaaaa gccagctcca tttctttcaa
    32881 tcgctcaact ggttccagca tagctttcag cagtgctttt tgtgtatttc tcgtaccgat
    32941 gacccaagca gcgatacggt tgatcgttgc atcaaagaaa tccagaccga tatttgtttt
    33001 ttccaacaat tcgttacgga ctaattcttt agcgatttcc tgcaattcat catccatcaa
    33061 aaccacatgg tctgaatccc aacgcactgg acgggaaaca tgcaacatga ttcctttgct
    33121 gaataaagct aatgaagata atttattgga aatcacttct gttggatgaa aatgtccagc
    33181 atctagacaa acaagtttgt cacgagtcaa cccgtaaccc atataaaatt catgggaacc
    33241 gactgtataa gcttcagcgc ctaaaccaaa tagtttgctt tctactgcat cttgtacata
    33301 ccgttcatct aactcttctg taaaaatttc atcgagtgat tccattaaac gttcccgtgg
    33361 ggtaaaacga tcaatcggat tatctttcat cccatcaggt acccaaaagt tattgatact
    33421 tgtttgccca gtttctttcc caaagtactc cgcgattttg cggcttcttt tcccgtgttc
    33481 gatccagtat tcacgaactt ctttatctgg atgagccaat gtaaagccgt ctttcatcat
    33541 tggatgcgaa aagaaagtcg gattgaagtc aagaccgatt tcttcttttt ttgcccactc
    33601 tacccatttt tcaaaatgtt tcggctcaat ttcatttaga tcgacagatt cttctgtatc
    33661 caaatagata gcatgaaggt tcaatttgtg tttgcccggt atcaacgaat aagccttctc
    33721 cagatcttgt ctaagctctt caggtgttcg tgcggctcct ggaaaattcc ctgtcgccat
    33781 gattccaccg gtcaattcgc catccggtgt caagaaacct ttgacatcat ctccttgcca
    33841 gcaatgcata gaaattttga tgtcctgtat cttttctagt acagcatctg tatctacacc
    33901 gatagttgca tatttttctt ttgcttgttc gtaatttctt tcaatcgtgt tcatacgatt
    33961 actcctttga ttttattcac gtaccattcc atcgtgtttt gcttattttc atctgggtaa
    34021 aaagtttcac atggaaacga ctgtttgatt acatttcttg cttgttctat attcgaaaac
    34081 tgacctgtac tgagcatttg catggcaaca ttcccgattg ctgtggcttc actaggtcct
    34141 gcttctacaa tgattccggc ataatctgct gtcaattgat tcagtagacg attattcgat
    34201 ccgcccccta cgatgtgcag cttttccaat ttcgttccag ttaagtgttc tactttgatc
    34261 agctcttgtg cgtaacaaag agcaagatta tcatacacac aacgagcgat agctcccgat
    34321 gagacaggga ctggctgctc tgtttcaaaa cagtattgct gaatctcttc tatcatattt
    34381 gggggattca aaaatcgagg gtcattgatt tccatcactt gctgcagcgg tttttctttt
    34441 gcagcaagct ctgccaattc cgcataactg tactcgtact cttgcattcg ggcaacttcc
    34501 tggatcaacc acatccccat gatgtttttc aaaaaacgca ccgtgttatg ggcaccccat
    34561 tcattcgtat aattttcaga aaaagcattg cgagaaacat tggcgatctt tgtttcaatc
    34621 cctaaaagcg accatgtccc actgctgata taaccccaac gttctccttg acctggcgtt
    34681 cctaatacag ctgacgccgt atcatgactt gctactgtga taaaggtcac atctggcaaa
    34741 tcatattttg aaaattgttc tttttgcaga tctcctagta tcgttcccgg ctctgtcaat
    34801 ggtggaaata attccttcga gacaccgatt ttttcaagaa gttctatttc ccattctttt
    34861 gtccctgcat tcagcagctg catagtagat gcattagttt tctccgtcac catgttacca
    34921 gtaaagacat accctaaata atcaggtatc agtaacaatt tatgtgcttt tgccaaattt
    34981 tctttttctt cagcaaacaa ttggaacaag gtattgaaag gctggatctg aatccctgtc
    35041 tgctcataca acttatctag agaatagatt tcagaaaact gttctactgc tccattggta
    35101 cgcttgtccc gataagcaat cggatcattt atccgatccc cttgttcatc aatcagacag
    35161 taatcgactg cccatgtatc gatccccgca aaaaggcgat caatccctgt ctgctttaat
    35221 ttttcaaatc caatcaaaat ctctttgata agataatcga tcttccagcg atcgtgaccg
    35281 tctttgttcc gaaagccgtt tttgaaccgg tgtacttctt ccaagatcaa agaacctttt
    35341 gcatccctcg tacttttcat tagtcgtccg cttgatgcac cgatatcgat ggcaacaaat
    35401 gttggattca tttctccacc tcttttactt tattgttgat tagcacttta ttaataaagc
    35461 gttatcattt attgacaatg ctatcataat acagaatctt atattttgct tcacactatt
    35521 ttggtatata attgcattat cttgacaagt tgggagtgtt tacatggatt actgggaaaa
    35581 agccaaccac tcatggtcgg aagactcaaa aagagtgatc ttgacgccta cacaaagaag
    35641 caggaattta tttttttata tccaagaaat cggttacttc aaagcaccaa tcccttacta
    35701 tactgaacga gcacatttac cttcttactt gataaagttt actttgtctg gcaaaggttc
    35761 gcttacatat aatgataatc agcaacagct aaaagctgga gacttatttt ttattgactg
    35821 ccaagaatat cagcgttatc aaacgatcag cgaagaacca tgggaaatgg attggatcca
    35881 tttgaatggt cagaacgtcc aatcatttta tcaggagttc acaaaagacg gccataacac
    35941 cttccattca acaggagatc catcagaaaa caagatccac catttgatca cagaattgat
    36001 ccacatgcaa caaggcacga atgcccgtac cgatttccag tcctctgtag ctatccacga
    36061 gttgctgaat gaactgattt tgcaaaagta tcagctagat ttcgaagaag aggatattcc
    36121 cgcctacgtt tacaaactga agcaatactt agacgaacat ttcaaagaaa cgattacact
    36181 tgatcaatta gaacaacaat tcttattgaa taaatatcag ctgagcaaag atttttccaa
    36241 atttatcggt tctccaccaa tcgattattt gatcagcaaa aaactttctt acgctaaaga
    36301 ccttcttcgg tatacagaat ttacggttcg aatgatttcc gaagaaatag ggattgagaa
    36361 ttttgcttac tttagccgcc tgttcaaagc aaaaacagga ctatcgccga gagactttcg
    36421 taaa
//
LOCUS       DASJUF010000017        36284 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJUF010000017 DASJUF010000000
VERSION     DASJUF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.017"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(140..2275)
                     /locus_tag="U4B62_000743"
     CDS             complement(140..2275)
                     /locus_tag="U4B62_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2140924.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2358..4013)
                     /locus_tag="U4B62_000744"
     CDS             complement(2358..4013)
                     /locus_tag="U4B62_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2140925.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4015..4329)
                     /locus_tag="U4B62_000745"
     CDS             complement(4015..4329)
                     /locus_tag="U4B62_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2140926.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4499..4759
                     /locus_tag="U4B62_000746"
     CDS             4499..4759
                     /locus_tag="U4B62_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN2140927.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            4773..5087
                     /locus_tag="U4B62_000747"
     CDS             4773..5087
                     /locus_tag="U4B62_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN2140928.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5817..6371)
                     /locus_tag="U4B62_000748"
     CDS             complement(5817..6371)
                     /locus_tag="U4B62_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN2140929.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6497..8426)
                     /locus_tag="U4B62_000749"
                     /pseudo
     CDS             complement(6497..8426)
                     /locus_tag="U4B62_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(8732..9448)
                     /locus_tag="U4B62_000750"
     CDS             complement(8732..9448)
                     /locus_tag="U4B62_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2140930.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9449..11272)
                     /gene="glpO"
                     /locus_tag="U4B62_000751"
     CDS             complement(9449..11272)
                     /gene="glpO"
                     /locus_tag="U4B62_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN2140931.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11286..12782)
                     /gene="glpK"
                     /locus_tag="U4B62_000752"
     CDS             complement(11286..12782)
                     /gene="glpK"
                     /locus_tag="U4B62_000752"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN2140932.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(12918..14378)
                     /locus_tag="U4B62_000753"
     CDS             complement(12918..14378)
                     /locus_tag="U4B62_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2140933.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14368..15663)
                     /locus_tag="U4B62_000754"
     CDS             complement(14368..15663)
                     /locus_tag="U4B62_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2140934.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(15995..16672)
                     /locus_tag="U4B62_000755"
     CDS             complement(15995..16672)
                     /locus_tag="U4B62_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN2140935.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16702..17793)
                     /locus_tag="U4B62_000756"
     CDS             complement(16702..17793)
                     /locus_tag="U4B62_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN2140936.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(17894..18328)
                     /locus_tag="U4B62_000757"
     CDS             complement(17894..18328)
                     /locus_tag="U4B62_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2140937.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(18912..20369)
                     /gene="ascB"
                     /locus_tag="U4B62_000758"
     CDS             complement(18912..20369)
                     /gene="ascB"
                     /locus_tag="U4B62_000758"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2140938.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20366..22261)
                     /locus_tag="U4B62_000759"
     CDS             complement(20366..22261)
                     /locus_tag="U4B62_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2140939.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22319..24094)
                     /locus_tag="U4B62_000760"
     CDS             complement(22319..24094)
                     /locus_tag="U4B62_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2140940.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24144..25640)
                     /locus_tag="U4B62_000761"
     CDS             complement(24144..25640)
                     /locus_tag="U4B62_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2140941.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25834..27456)
                     /locus_tag="U4B62_000762"
     CDS             complement(25834..27456)
                     /locus_tag="U4B62_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2140942.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(27784..29097)
                     /locus_tag="U4B62_000763"
     CDS             complement(27784..29097)
                     /locus_tag="U4B62_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2140943.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29342..29566)
                     /locus_tag="U4B62_000764"
     CDS             complement(29342..29566)
                     /locus_tag="U4B62_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140944.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29586..30008)
                     /locus_tag="U4B62_000765"
     CDS             complement(29586..30008)
                     /locus_tag="U4B62_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN2140945.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(30150..30860)
                     /locus_tag="U4B62_000766"
     CDS             complement(30150..30860)
                     /locus_tag="U4B62_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140946.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(30878..31375)
                     /locus_tag="U4B62_000767"
     CDS             complement(30878..31375)
                     /locus_tag="U4B62_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2140947.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31412..31864)
                     /locus_tag="U4B62_000768"
     CDS             complement(31412..31864)
                     /locus_tag="U4B62_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2140948.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(31999..32604)
                     /locus_tag="U4B62_000769"
     CDS             complement(31999..32604)
                     /locus_tag="U4B62_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2140949.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33054..33674)
                     /locus_tag="U4B62_000770"
     CDS             complement(33054..33674)
                     /locus_tag="U4B62_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2140950.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33676..34545)
                     /locus_tag="U4B62_000771"
     CDS             complement(33676..34545)
                     /locus_tag="U4B62_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN2140951.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34568..34975)
                     /locus_tag="U4B62_000772"
     CDS             complement(34568..34975)
                     /locus_tag="U4B62_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2140952.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(34994..36283)
                     /locus_tag="U4B62_000773"
     CDS             complement(34994..36283)
                     /locus_tag="U4B62_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2140953.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        10287 a         7790 c         6063 g        12144 t
ORIGIN      
        1 gtttaccata catttcattt cttttcacat aaagtgttcc taaatttttc ctaagaaaaa
       61 gaacaaaaca tttctcatca gcaaatgaaa aatattttgt tctttttatc agttgttctt
      121 tttatttttt atctcttaat tatgttgctc tttccaatac gacaatgctt ttcattcctc
      181 tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc atctccatcg ttttcaaatg
      241 gatataagat tacatgatca aattccagtc cttttgctag actcaaagga atgatttggc
      301 agttttctaa tccatatgaa ttttttttaa tcgattcttg tagcgtttgg gtttcctctt
      361 ctgtttgggt gataactgcc gctttttctc cttttagaga aaccaaaatc tcagctagta
      421 ttttcaacaa ttcttcagag gatcgaaaag agcggtattc gggttctttt ccttgcggtc
      481 ggatcgatac gatgtcgatc ttctcttgat ccacagcata ggttttgaaa agctctgtga
      541 ttgctccgct agaacggtaa ctatttctaa gatcataccg atggatcggc agatggtagt
      601 cgtcgaaaca acgcataata tcagcaaaag tagtggaaga attaaagatt gcctgattct
      661 cgtcacctac taaagtaaag tgagtttttg gaaataattc gatcaataga cctaattgtg
      721 ccagggtata atcttgtact tcatcaatca atagatagcg aaatgctggt acatttacct
      781 tttcaacaag taaatgacga atagctaata taatgactgc ttcatcaaca gtacgtgtgg
      841 gttgccaagc ataagctgag ccatatcgtt tttcataaat tctttcaaac aactgatgct
      901 catccaccca tgccatttct tcgatttgtc tggttatttt tcgatatttt ttttgtaaaa
      961 gtttccttgc ataagctaca atactttgtt ccgaatcatc agaaatcaat ttaccgaata
     1021 attccaactg cctatcttct gtcaacgaga gtacctgatt ctgcacagca ggactttttg
     1081 cttgtttcaa aagatgattc ttccattcgc tcatcagtgc ctttttcgtt gcttgaagcc
     1141 tatcgatcaa tttaggatga gcaggaactt tctcatatat tttttcgatc gtttttttag
     1201 aaaggactgt ttttccattt ttagtaagtc cacggataaa attcggatga tctaaaaaca
     1261 aaggatctga ttgttttaga ttatcgataa actttttgct acgcaaacgt tctgtctgct
     1321 ccgatacatt ttctcccgtg atacgtttga aataagcctc ttcaccttct atttcctctg
     1381 ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag tggatttctt tcacctaatg
     1441 atggtaggac attcgaaata tagtcgataa accgatgatt aggtgataga atcagcaagt
     1501 catcggatgt catcgtatct cgatataaat agagcagata cgcaatacgc tgcatgatag
     1561 cagaggtttt accacttcct gctacgccat ttaccaagat attttgactt ttcacatccc
     1621 gaatgattgt gttttgttcc gattggatcg tagcagtgat agccttcatt tcattggtct
     1681 catcttggcc taagacttct aataatacgt catcttgaat agctacagac gtatcaaaaa
     1741 acttgaccaa acgatctctt tccaaaagaa attgtcgtct ttctttaata accgtatcga
     1801 ttttattttg acgaaccgta taagaagatg gaccaatcat gttattgtaa aaaagttcgg
     1861 caattggtga acgccaatca taaacaagat tttcatctgc atcatctgtg aaatttgctg
     1921 taccgatata aaaggattct tttggctcat tatccagaaa gtctacaacg acttttccaa
     1981 agtagggact tttcaataaa cgttcgatct ttgctagctg attttctgcg tttttctgtt
     2041 tgatattcat ctgatcgatt tctctatttt tcatttcaat catcgaaaaa gtatccaggt
     2101 tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc catttctttc aacgactggt
     2161 ttccttctat ttttgccgta tccatcgatt cccgcagttc gtttttttta tcaattagct
     2221 tttgatacac gttcttcaga tatgcttctt ctaactgttt ttgtgtgttg accatacaca
     2281 ttctcctctt tttttaaagt ggtggaaccg attagtttac ctcttttaag agtcgaatgc
     2341 aagcttttta acgagtatca tttgttagga tatatcagtt cttcaggtcg gacagtttga
     2401 tataaagaaa atgcaccatc aaggtttttt acgtggtagc cagactgctt caagattctt
     2461 tccgctagat agctacgtaa tccgctatga cagctgacga tatattcttg ttggctgtct
     2521 aattcattta atcggtcccg aagttcattc aaagggatat gtttggcttc cttgaatttg
     2581 cccttttcca attcttctgc tgttcgcaca tctaataaaa tttttccttt tgctagttct
     2641 gtcggcaatt catgccattg tacggtttca ctaagccctt ctgcaatgtt cattgctgca
     2701 tatcctagca tgtttactgg atctttggct gagccaaatg gcggtgcata cgtcaattct
     2761 aattccggta aatcgaagat cgtcaaatgc cctttgattg ctgttgctaa aatatcgatc
     2821 cgtttatcca caccttttgc cccaacacct tgtgcaccat aaatctcgcc agtctctgga
     2881 tggaagatca acttcagtaa aatatctgtt gcttctggat aatagcttgc atgatctttt
     2941 cctgatacat gaatgacttc aaaagacagt tcgttttctt tggctgttct ttcacttaaa
     3001 ccggtggatg cggcagtcat tccaaacgtt cgtataattg ctgttccaat accgccttga
     3061 tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc taccttgacg attggctggt
     3121 gaagcaagag aaataagtgc atcttttcca gtgatttgct gttttacaac gatagcatcc
     3181 cccaccgcat aaatatcagg aatattcgtt tgataacgtt cgtctactag gatacccccg
     3241 cgtatgccta attcgattcc agcggtcact gctagctggt tatcaggacg aacaccaatg
     3301 gataaaataa ctaaatcagc agggatggta cgcccatcat ccagtcgcaa tcgtttcccg
     3361 tggtcttcaa atccagcgac agcatgagaa gtaatcactt ctactccttg atgagacaat
     3421 tctttttcta taaaagctgc catttcttca tccagtgttg gaagaatatg tggtgcttgt
     3481 tcgatcacca tgacttctaa acctcttctt tttaggtttt ctgccatttc caatccgatg
     3541 aatcctgcac caacgatcac ggcacgcttt ggctgttttt ctaatgcatg catcacttta
     3601 tcgatatctg gtacatttct aatagaaaaa acattgtcgg cttctgttat ccctggaatc
     3661 gatggaacaa ctggttttgc accaggagat aaaaccaatg catcgtactg ttcaatgtgt
     3721 ttttgaccct catgtacgac ttctaccgtt ttgttttcgg gaaagatggc tgtcacttca
     3781 tgatttggac gcacatctaa acaaaaacga gcggataatg attctggcgt ttggacaaga
     3841 agattttccc gttcactgat ttctcccgaa agataataag gcaagccgca atttgcaaaa
     3901 gaaacatacg gtcctttttc aaaaacaacg atttctgcat cttccattaa tcggcgaagc
     3961 cgtgtagcag cagacattcc acctgctaca cctccgacaa taacgatttt catcttattt
     4021 ccctcctact gttcgatcaa accattgatt catgccacca cgtacattca ctacatcata
     4081 accagatttc tttaattctt tagctgcttg tttactcctc atccctgatt gacaaatcac
     4141 atagacggtc ttttctttgt ctccatcata tctgtttata ctttgtaatg gaacattttt
     4201 agctcctttt atatgcccgc cccggtattc tgcaggtgtt ctaacatcaa gtaacgctgg
     4261 attttccaaa agcttttcct ttaattctgg gactgttatc gattttattg attgaaatag
     4321 caaattcatt ttctgcacct catttacatt tttataaata cctcatgggg tatattaaac
     4381 aggagcttta ttaatatgtc aaccagaatg actcttcatt tttttcacta ctttttatat
     4441 ttgcataaaa taccgataag gggtattatc taattataaa aatagaggag aatgtgttat
     4501 ggagattgat aataaaaata tcctgaaccg tttgaaacgt acagagggac aaattcgtgg
     4561 tatccaaaaa atgatcgaag acgaaaaaga atgtatggat gtcatcactc aattaagcgc
     4621 tgttcgttct agtattgatc gcgtaatggg catgatcgtt gcagataatc tgaaaaattg
     4681 ctttgagaaa cctgatagta atccagaaga acaagcggct aaactagaac aagcgatcaa
     4741 gatgattatt aaaaagtaaa ggaatgattt caatgaaagt cgttttccat attgatgaat
     4801 tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct gataaaagct tctccagaaa
     4861 cgacgattgt cgtatctgta aatggcatcg cgattaccgg ttatctagac tctgccaatg
     4921 cagaattctt agatttgcaa ggagtcgttt ttcacgcttg cgccaatgct atgcgtgcta
     4981 atcatatttc agaaagttct ttacctgaac aagtaatcgt tgtaccagca ggtgtccttg
     5041 atttagttga actgcaatcc caaggttatg cttatatcaa gccttagtca gtagaggttg
     5101 cgacagaagt gatcagcttc aagaaatttc ggcttatttt tcgaagaagc tacttctgga
     5161 aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc aagtaataag aacagccaaa
     5221 caaaaatagc ttctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     5281 gaacaccgtt taaggaagag gttgtgaaat cagcggtttg acctaagtat tagagaaaaa
     5341 aacgggaaaa tagctcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     5401 ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt agtttcgagt attaagaaaa
     5461 attttgtgaa aatggatttc tacatttttg cataaatttg gcttaatacc gagaaactgg
     5521 cttttgaata ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag
     5581 aaacaaacgg aaaaatagct gctcatattt tgccgttttt tgagctaata ccgaaggtca
     5641 gctgattgaa caccgtttac caagaggttg tgagcctacc atttaactcc gaatcacaag
     5701 aaataatttc aaaaacaact tctcctgttt ttggttcggt tggcttatta tcgagaagta
     5761 gctactggga gtctatttat caaaaaaagg aatgcgacat aggtcacacc ccttttttat
     5821 tttctttgaa acatctttcg gtactcgaca gcatacaaga caaaagattg taaaagaccg
     5881 taagtgatga cggcaaaaat gactctcacc cacagcgttg gtaattctgg gaaaagcagc
     5941 aaaaggatca tgccaacata gatgacagca gtcgctactt tgccatacca cttcgctcca
     6001 cttaatttga tatttttaga tagcccgatt aatccagcta caaacataaa gccttctttt
     6061 atcaagaata gaccaaacaa cagccaaagc cacggtcgat accatgccat agacaaaatc
     6121 agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca ccttccctaa gtctgttacc
     6181 tgattatatc tccgagcaat gaccccgtct agataatcag acaaacctga gacaacaatg
     6241 accgctcctg ctatataaaa tgactgtctc agcacaaaat aaagataaat cggcagcaaa
     6301 aaaatacgga atatcgataa aagattcgga atcgtttgta tttctttacg ccagtctgtg
     6361 cttaatttca ttttatcgcc ctacctcctc agcactcttt tctttttatt ttagcttaca
     6421 cggggaataa aaccaatgat ttcactgttt ttatttttct ttacagacac tgatattatg
     6481 agctaatttg agataatcac ttgctgtttt catcgtttac tcttctttct tcctgtcgtt
     6541 tgcgcatttc ttttactagt actttatgag ctttttttga tcctcgctct aggtcacgct
     6601 gccgtttacg tgttttataa tcgataaaca gactgtctaa atcgtaacct gtcgggtaca
     6661 gatcttctgc tgaacgcacc agacgaacac gctgacgagg cacgtggatt atttttttgt
     6721 ctaaatatac atcaatcgta tcttcaccta tatcttcata aactaacgct tctttttggt
     6781 attcattgac aaatatccga tcaccttttg caaagagctg acgttctttt ttattctcct
     6841 tcctatttgt aactgctgtc ttaggaagaa attctttttt ctgtgtccga taggcttttt
     6901 ccgccaagta acgctctgct tgatggatca atttcatcga catatccatt ttatgagcaa
     6961 tccacaaagc ttggctttct cctgtttctt ccaacccgca actgatattt aggacgaagt
     7021 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     7081 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     7141 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     7201 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     7261 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     7321 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     7381 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     7441 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     7501 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     7561 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     7621 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     7681 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     7741 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     7801 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     7861 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     7921 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     7981 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     8041 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     8101 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     8161 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     8221 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     8281 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     8341 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     8401 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     8461 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     8521 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     8581 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     8641 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     8701 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     8761 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     8821 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     8881 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     8941 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     9001 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     9061 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     9121 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga
     9181 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     9241 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     9301 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     9361 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
     9421 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     9481 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     9541 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     9601 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     9661 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     9721 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
     9781 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
     9841 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
     9901 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
     9961 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
    10021 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
    10081 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
    10141 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
    10201 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
    10261 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
    10321 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
    10381 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
    10441 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
    10501 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
    10561 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
    10621 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
    10681 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
    10741 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
    10801 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
    10861 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
    10921 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
    10981 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
    11041 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
    11101 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
    11161 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
    11221 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
    11281 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
    11341 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
    11401 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
    11461 gccgttgttt ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
    11521 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
    11581 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
    11641 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
    11701 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
    11761 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
    11821 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
    11881 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
    11941 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
    12001 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
    12061 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
    12121 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
    12181 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
    12241 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
    12301 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
    12361 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
    12421 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
    12481 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
    12541 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
    12601 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
    12661 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
    12721 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
    12781 attgtgattc cctccatcat atgcaaacgc tttctttaac tactcttatc atactactgt
    12841 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
    12901 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
    12961 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
    13021 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
    13081 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
    13141 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
    13201 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
    13261 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
    13321 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
    13381 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
    13441 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
    13501 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
    13561 tctactatcg aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt
    13621 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
    13681 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
    13741 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
    13801 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
    13861 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
    13921 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
    13981 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
    14041 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
    14101 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
    14161 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
    14221 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
    14281 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
    14341 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
    14401 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
    14461 ttcaaacgtc catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc
    14521 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
    14581 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
    14641 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
    14701 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
    14761 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
    14821 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
    14881 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
    14941 ccacatctga ttgtcgggac gagaatgatt agccagcaaa acaagatcat agttctcctt
    15001 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
    15061 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
    15121 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
    15181 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
    15241 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
    15301 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
    15361 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
    15421 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
    15481 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
    15541 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
    15601 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
    15661 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
    15721 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
    15781 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    15841 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    15901 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    15961 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    16021 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    16081 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    16141 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    16201 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    16261 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    16321 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    16381 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    16441 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    16501 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    16561 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    16621 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    16681 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    16741 tggcaaataa agctctttat atgctttttc aaaaggtttc gttacaaatt catcctcacg
    16801 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    16861 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    16921 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    16981 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    17041 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    17101 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    17161 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    17221 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    17281 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    17341 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    17401 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    17461 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    17521 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    17581 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    17641 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    17701 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    17761 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    17821 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    17881 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    17941 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    18001 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    18061 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    18121 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    18181 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    18241 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    18301 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    18361 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    18421 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    18481 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    18541 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    18601 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    18661 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    18721 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    18781 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    18841 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    18901 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    18961 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    19021 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    19081 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    19141 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    19201 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    19261 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    19321 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    19381 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    19441 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    19501 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    19561 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    19621 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    19681 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    19741 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    19801 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    19861 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    19921 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    19981 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    20041 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    20101 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    20161 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    20221 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    20281 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    20341 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    20401 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    20461 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    20521 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    20581 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    20641 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
    20701 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    20761 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    20821 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    20881 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    20941 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    21001 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    21061 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    21121 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    21181 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    21241 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    21301 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    21361 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
    21421 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    21481 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    21541 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    21601 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    21661 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    21721 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    21781 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    21841 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    21901 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    21961 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
    22021 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
    22081 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
    22141 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
    22201 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
    22261 ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt
    22321 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
    22381 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
    22441 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
    22501 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
    22561 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
    22621 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
    22681 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
    22741 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
    22801 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
    22861 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
    22921 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
    22981 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
    23041 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
    23101 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
    23161 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
    23221 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
    23281 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
    23341 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
    23401 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
    23461 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
    23521 tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
    23581 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
    23641 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
    23701 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
    23761 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
    23821 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
    23881 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
    23941 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
    24001 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
    24061 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
    24121 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
    24181 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
    24241 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
    24301 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
    24361 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
    24421 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
    24481 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
    24541 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
    24601 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
    24661 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
    24721 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
    24781 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
    24841 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
    24901 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
    24961 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
    25021 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
    25081 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
    25141 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
    25201 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
    25261 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
    25321 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
    25381 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
    25441 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
    25501 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
    25561 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
    25621 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
    25681 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
    25741 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
    25801 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
    25861 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
    25921 aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat
    25981 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
    26041 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
    26101 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
    26161 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
    26221 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa
    26281 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
    26341 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
    26401 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
    26461 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
    26521 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
    26581 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
    26641 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
    26701 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
    26761 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
    26821 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
    26881 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
    26941 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
    27001 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
    27061 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
    27121 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
    27181 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
    27241 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
    27301 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
    27361 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
    27421 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
    27481 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
    27541 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
    27601 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
    27661 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
    27721 aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc
    27781 tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat
    27841 gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa
    27901 acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa
    27961 tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa
    28021 tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg
    28081 agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg
    28141 tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa
    28201 taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat
    28261 cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc
    28321 aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc
    28381 ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat
    28441 ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc
    28501 gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa
    28561 aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt
    28621 cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc
    28681 agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa
    28741 gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca
    28801 tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg
    28861 tgccgttcca attggataac gggctaatac ccccattaaa acacagccga tcgcactggc
    28921 taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg
    28981 atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg
    29041 ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt
    29101 ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa
    29161 aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta
    29221 agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca
    29281 ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa
    29341 atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg
    29401 tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc
    29461 cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg
    29521 ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt
    29581 tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa
    29641 aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg
    29701 tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct
    29761 ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg
    29821 ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca
    29881 attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt
    29941 gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact
    30001 gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa
    30061 agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa
    30121 tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt
    30181 agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg
    30241 attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct
    30301 gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag
    30361 atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct
    30421 gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg
    30481 atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag
    30541 atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa
    30601 gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac
    30661 tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt
    30721 gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg
    30781 ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta
    30841 tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata
    30901 gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc
    30961 cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg
    31021 ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac
    31081 cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca
    31141 tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga
    31201 tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg
    31261 cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat
    31321 ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc
    31381 gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga
    31441 caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt
    31501 ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa
    31561 tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg atttttttat
    31621 tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg
    31681 cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat
    31741 tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt
    31801 cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag
    31861 ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg
    31921 caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt
    31981 cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa
    32041 ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc
    32101 catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc
    32161 aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa
    32221 aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg
    32281 cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt
    32341 cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc
    32401 tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt
    32461 ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc
    32521 tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt
    32581 gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg
    32641 taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa
    32701 ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat
    32761 aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg
    32821 agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc
    32881 cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg
    32941 agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc
    33001 tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta
    33061 ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag
    33121 tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg
    33181 atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat
    33241 acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca
    33301 cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc
    33361 tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga
    33421 tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag
    33481 aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg
    33541 gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt
    33601 tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg
    33661 aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag
    33721 tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg
    33781 aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac
    33841 taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc
    33901 ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat
    33961 ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc
    34021 gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg
    34081 tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt
    34141 tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt
    34201 cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc
    34261 gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc
    34321 atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat
    34381 atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc
    34441 gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt
    34501 caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct
    34561 tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca
    34621 ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct
    34681 tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca
    34741 aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc
    34801 caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt
    34861 tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt
    34921 cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt
    34981 tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg
    35041 ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga
    35101 tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga
    35161 tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga
    35221 aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg
    35281 caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag
    35341 gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc
    35401 ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca
    35461 cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg
    35521 ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca
    35581 tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga
    35641 tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt
    35701 caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt
    35761 tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac
    35821 caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca
    35881 tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac
    35941 gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta
    36001 aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg atcccttgca
    36061 tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg
    36121 aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc
    36181 ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg
    36241 cctgagtcaa aatattttcg atgataaaat tgataacgcc catg
//
LOCUS       DASJUF010000018        34469 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJUF010000018 DASJUF010000000
VERSION     DASJUF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.018"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..399
                     /locus_tag="U4B62_000774"
     CDS             82..399
                     /locus_tag="U4B62_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140954.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            332..811
                     /locus_tag="U4B62_000775"
     CDS             332..811
                     /locus_tag="U4B62_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEN2140955.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            828..1004
                     /locus_tag="U4B62_000776"
     CDS             828..1004
                     /locus_tag="U4B62_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140956.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1261..1692
                     /locus_tag="U4B62_000777"
     CDS             1261..1692
                     /locus_tag="U4B62_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140957.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            1698..2015
                     /locus_tag="U4B62_000778"
     CDS             1698..2015
                     /locus_tag="U4B62_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN2140958.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2134..2820
                     /locus_tag="U4B62_000779"
     CDS             2134..2820
                     /locus_tag="U4B62_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2140959.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            2795..4144
                     /locus_tag="U4B62_000780"
     CDS             2795..4144
                     /locus_tag="U4B62_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2140960.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4135..4608
                     /locus_tag="U4B62_000781"
     CDS             4135..4608
                     /locus_tag="U4B62_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2140961.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            4583..5110
                     /locus_tag="U4B62_000782"
     CDS             4583..5110
                     /locus_tag="U4B62_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEN2140962.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            5125..7437
                     /locus_tag="U4B62_000783"
     CDS             5125..7437
                     /locus_tag="U4B62_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN2140963.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            7684..8001
                     /locus_tag="U4B62_000784"
     CDS             7684..8001
                     /locus_tag="U4B62_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEN2140964.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            7998..8159
                     /locus_tag="U4B62_000785"
     CDS             7998..8159
                     /locus_tag="U4B62_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2140965.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            8162..8461
                     /locus_tag="U4B62_000786"
     CDS             8162..8461
                     /locus_tag="U4B62_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140966.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            8461..8775
                     /locus_tag="U4B62_000787"
     CDS             8461..8775
                     /locus_tag="U4B62_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN2140967.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            8768..9298
                     /locus_tag="U4B62_000788"
     CDS             8768..9298
                     /locus_tag="U4B62_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN2140968.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            9295..9564
                     /locus_tag="U4B62_000789"
     CDS             9295..9564
                     /locus_tag="U4B62_000789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140969.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            9494..9697
                     /locus_tag="U4B62_000790"
     CDS             9494..9697
                     /locus_tag="U4B62_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN2140970.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            9694..9981
                     /locus_tag="U4B62_000791"
     CDS             9694..9981
                     /locus_tag="U4B62_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140971.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            10468..10881
                     /locus_tag="U4B62_000792"
     CDS             10468..10881
                     /locus_tag="U4B62_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN2140972.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            11501..11665
                     /locus_tag="U4B62_000793"
     CDS             11501..11665
                     /locus_tag="U4B62_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140973.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            12077..12622
                     /locus_tag="U4B62_000794"
     CDS             12077..12622
                     /locus_tag="U4B62_000794"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2140974.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            12623..12940
                     /locus_tag="U4B62_000795"
     CDS             12623..12940
                     /locus_tag="U4B62_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2140975.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(13097..13261)
                     /locus_tag="U4B62_000796"
     CDS             complement(13097..13261)
                     /locus_tag="U4B62_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEN2140976.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            13363..13884
                     /locus_tag="U4B62_000797"
     CDS             13363..13884
                     /locus_tag="U4B62_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN2140977.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            13871..14344
                     /locus_tag="U4B62_000798"
     CDS             13871..14344
                     /locus_tag="U4B62_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN2140978.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(14351..14509)
                     /locus_tag="U4B62_000799"
     CDS             complement(14351..14509)
                     /locus_tag="U4B62_000799"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEN2140979.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            14602..15363
                     /locus_tag="U4B62_000800"
     CDS             14602..15363
                     /locus_tag="U4B62_000800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2140980.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            15583..16902
                     /locus_tag="U4B62_000801"
     CDS             15583..16902
                     /locus_tag="U4B62_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2140981.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            16918..18138
                     /locus_tag="U4B62_000802"
     CDS             16918..18138
                     /locus_tag="U4B62_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2140982.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            18173..18850
                     /locus_tag="U4B62_000803"
     CDS             18173..18850
                     /locus_tag="U4B62_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN2140983.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            18847..20040
                     /locus_tag="U4B62_000804"
     CDS             18847..20040
                     /locus_tag="U4B62_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2140984.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            20055..20381
                     /locus_tag="U4B62_000805"
     CDS             20055..20381
                     /locus_tag="U4B62_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2140985.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            20385..20720
                     /locus_tag="U4B62_000806"
     CDS             20385..20720
                     /locus_tag="U4B62_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2140986.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            20717..21112
                     /locus_tag="U4B62_000807"
     CDS             20717..21112
                     /locus_tag="U4B62_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140987.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            21109..21480
                     /locus_tag="U4B62_000808"
     CDS             21109..21480
                     /locus_tag="U4B62_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140988.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            21496..22056
                     /locus_tag="U4B62_000809"
     CDS             21496..22056
                     /locus_tag="U4B62_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2140989.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            22181..22492
                     /locus_tag="U4B62_000810"
     CDS             22181..22492
                     /locus_tag="U4B62_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140990.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            22516..22707
                     /locus_tag="U4B62_000811"
     CDS             22516..22707
                     /locus_tag="U4B62_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140991.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            22707..26555
                     /locus_tag="U4B62_000812"
     CDS             22707..26555
                     /locus_tag="U4B62_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN2140992.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            26556..27428
                     /locus_tag="U4B62_000813"
     CDS             26556..27428
                     /locus_tag="U4B62_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEN2140993.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            27425..28474
                     /locus_tag="U4B62_000814"
     CDS             27425..28474
                     /locus_tag="U4B62_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140994.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            28478..29302
                     /locus_tag="U4B62_000815"
     CDS             28478..29302
                     /locus_tag="U4B62_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140995.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            29320..31380
                     /locus_tag="U4B62_000816"
     CDS             29320..31380
                     /locus_tag="U4B62_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN2140996.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            31397..31546
                     /locus_tag="U4B62_000817"
     CDS             31397..31546
                     /locus_tag="U4B62_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140997.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            31543..31989
                     /locus_tag="U4B62_000818"
     CDS             31543..31989
                     /locus_tag="U4B62_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2140998.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            31991..32128
                     /locus_tag="U4B62_000819"
     CDS             31991..32128
                     /locus_tag="U4B62_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN2140999.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            32163..32405
                     /locus_tag="U4B62_000820"
     CDS             32163..32405
                     /locus_tag="U4B62_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HEN2141000.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            32421..32618
                     /locus_tag="U4B62_000821"
     CDS             32421..32618
                     /locus_tag="U4B62_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2141001.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            32629..33645
                     /locus_tag="U4B62_000822"
     CDS             32629..33645
                     /locus_tag="U4B62_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN2141002.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            33760..>34469
                     /locus_tag="U4B62_000823"
     CDS             33760..>34469
                     /locus_tag="U4B62_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141003.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFG"
BASE COUNT        12112 a         5763 c         7645 g         8949 t
ORIGIN      
        1 acacgaacgc cggcaagcat gatcgtgtca gggaaataaa ctatctttct atattttacc
       61 acagaaagga atgaaccgta aatgctaatt gcaacggata cactagaccg catcttttta
      121 aaagacgaat acaaactgcg caatatagat gcgtcaggaa ttttagtatt tgatctttat
      181 gacaatggaa aaattggtat ctatcaagca agtgatattg aagaaacaaa ccttgctttc
      241 gaacaaattg atgattctgt ggaattggat ttagatgagg caatcctagc ttttgaacaa
      301 attgcaaaat tattaaagga ggcacaaaag aatggcaact ctttaccaac tcagcgagtc
      361 atatatcaaa gtcctggaac tggcagaaca attggatgaa gaaattcttc gcgatactct
      421 cgattcgatt aatgaagcga tcgagtataa ggcagaaaac ttagcaaaaa tagttaaaga
      481 agtagaaggg aaagctgagt taatcgatag tgaaattaaa cgtctgcagg aacgtaagac
      541 atcgcttttg aacaatgcta agagtatcaa gcactattta caagaggaaa tggaaaagac
      601 tggtaaaacg aagattaaag gtgaattatt caacattgga attcaaaata atccagtatc
      661 ggtcaatgta atcaatgaga acttaattcc aaaaggattc tttacccctg tgcctcccaa
      721 attggataaa aagcaattga aggaggaact gaagcacgga gatattcctg gtgctgaact
      781 cgtccaaaca aaaggtttga ggataagatg atgaaggagg agaaataatg gaacacgaaa
      841 acgtaaaaga cgctttgaaa gctgctatcg aaatcgcgga agctaaagga ataaaggttg
      901 acggcaaacc agcaactgtc catgacattc aaaatttgac gaaagaacat ctttatttta
      961 tcgccgactt gcttggtttg tcggaattgt atttagacaa ataaccccac acggaaggag
     1021 tggctagggt ggttgtatta caagtaatag tctgcgtaac attcatttgg tcgtgcatac
     1081 cggtttgtat atatatcatg aagaagcttc gccctgagtc ttacaaatta tacgcaattt
     1141 acagcataac agtagctctt gcatttacat tgtttgcaat ttctaggctt ccgttttaat
     1201 cgtgggaatg aatttttaat gaactaaatt atctataaag caaaaaaagg agaaacaaca
     1261 ttgaaaaaca aactagcaaa aacaacagca atcatcggac tagcactagg tagcggcgtt
     1321 atcggctacg cagcaagcaa tgcgtttcaa gatttggaca cgatcaaagc aaatttcaac
     1381 acagtcctac aatacggtca aacgaaatcg caacgtgtgt cagaacttga atcacagcta
     1441 tccaacaaca ctcgcacaca ggagcagctg aaagccgaaa ttgagcaaat caaatcggac
     1501 aagcagaagg aaattgaagc caagcaacga gaagtcgaac aaaagcaaca ggagatcgct
     1561 acaaagcaac aggaagccga tagcttgcgc caacaactat ccacagtgca aaacgacaag
     1621 gagcagttag aacagcgcgt gagcgagtta cggcagtata cggatcaaaa ggtaggggag
     1681 ttggggaaat gaaagagttg actatttatg acccaaatat ttcatgggct aaacacacaa
     1741 ttaaagtttc ttttatgatt tggggttaca aaggatatgt tacttacaaa gtgggcggta
     1801 acactaaggg tttatcatta atagctattg attcagatga tctatatgat gctaatttcg
     1861 aggataaccc agttaatttc cgtgaccttg atgaagattg gttctctatg gaacttacta
     1921 acgataaagg cgacagtaca cttgtagaag atgaattcga cagactaggt gattatattg
     1981 tcggtgttga aattattgcg cacgaaccag agtaggagag tgaatacaaa atgattaaaa
     2041 gaatcactat atcagttgaa gaagatagtg gatcaacaat gcagcatacc gacaatcgaa
     2101 aaactttttt aaccaataga aaggagaatt ttgatgcaga ttaagaaggc gacggatatc
     2161 gaacaaacca agggaacata tttgatctac ggtgctccag gaaaaggtaa aacttcaacg
     2221 gctaaatatt ttccagggaa aacactgatc cttgatattg atcggacttc aaaagtcctt
     2281 agaggtgaga aaaatgtcga tatcgtatat atcgacaacg aagatacctg gaacgactgg
     2341 ggcaacacgt tggctgattt aactaccaat tatgtaggtg tttatgacaa cgtggttgtc
     2401 gataatgtca gcgaattgga acggtgtatt ctgtccagtt taggcgcaga aggaaaaaac
     2461 aacggcgttc cttcgcaagg cgattatcaa tatatgcaat ttagaatggt gaacagccta
     2521 cgctatatga aaaatttaga cagcaacctt atctggaccg catgggaaga aatcgatttg
     2581 tggacggatt cggatggatc ttcttacaac atcgctttgc cgcagatcaa tcgaaagatt
     2641 cgaaacaata ttttgggatt atgtgacgtt gttggtcgat tgatggtaaa ggaagacgat
     2701 gaacgcggat ttattttaca agccacagat tcaacctatg ccaaaaatca attggataat
     2761 agatctggct gtaagcaaag tgagctgatt ttagatggaa ttgtacgact accagaatga
     2821 tttagtcgat cgtgcaagac aagcctatgt tgatggttac aaggcaccat gtatcgtatc
     2881 accgtgcggt tctggaaaat cagtcatggt tgcagagatc gctaaacgaa ccacacgaaa
     2941 aggcaacaga gtgttgttct tggtccatcg tcgtgaattg attgatcaga tcaaagatac
     3001 gtttcaaaag atgggcgtag ataatcagct tgtaaatttc ggcatggttc aaacagtcgt
     3061 acgacatctt aaaaccatca agaaaccaca actgatcatc actgacgaaa accatcacgg
     3121 gttagcagcg tcctacagaa aaatctatga gtattattcg gacgttccgc gattaggttt
     3181 taccgcgaca cccatccggt taaacggtag tgggttgggg gatgtgaacg acttgctaat
     3241 tgaaggagta tcggctaaat ggttgattga gaatcataga ttagctcctt atgaatatta
     3301 tgcacctaaa ttgatcgata cagccgaatt aaagaaagct tcgactggag attttacaaa
     3361 gaaatcaatg gataaggcag tgaagaatac tatttatggc gacgtgttga aacattataa
     3421 aacgttagca gaaggtgaac aggcaatagc atactgtcac agtatcgatg caagtaaaca
     3481 cactgctgaa atattcaacg atgctggtta taaagcaaca catatcgatg caaaaactcc
     3541 aaaagatgaa cgtgcagata ttatcgaagc gttcagaaat catgaaatta aaatcctatg
     3601 caatgttgat ttgatcggag aagggtttga tgttcctgat tgttcgactg taattatgtt
     3661 gagacctact caatcgttat cattgtacat tcagcaatcg atgaggggga tgcgttaccg
     3721 accagataaa acgtcaatca tcattgacca tgtgggtaat gtgaaccgtt ttggattgcc
     3781 tgatatggat cgtgaatgga gtttagatac aaaaaagaaa acaaaaactg atagtgattt
     3841 atctatcgtt caatgcgcgt tttgttttgg tgcttacgaa cggccaaaag gcgataatat
     3901 ttgtccgtat tgcggagagt tgcagccgat tgaagaacga aagtcagagt tagaaatcga
     3961 tgaatcggct gaattaatga aagtcggaga aacgaaaatt actttgaatt ttgagaacaa
     4021 taaatattac aacatgacgg aagatgaagc agaaagtata gaggacttat atgccatagc
     4081 aagagctaaa ggatttaaac ctggttgggc ttatatggct gctaagagga aggggtggtt
     4141 gtaggaatgg gcggtatttt tggtaaaaaa gcagaagatg ttcaaggttc aataataaat
     4201 ggcgtaacaa taataggtga tactggaaaa agagatagtt caggcaatca aattgtaatc
     4261 gctagaaata aaaataacga agtttttgaa tcttctctat ctaatataaa aagaggtgtt
     4321 gttaacggga caggcgaaga gagtggctac gttaatttaa aaaaagctaa tgaaaaatac
     4381 catgttgaag gaacttacgt taaggctttg tctgtcggtg gaacaaaagg cgttaattgg
     4441 gataaacgaa gaaataaatg gaaagcacga ttatatttca aaggagaata tgtcttacaa
     4501 aaggatttca acaccaaaga agaagcaatc caagcacgac ttgcagcaga agaaaaatat
     4561 ttcaaaccta tcttagaaaa atatgaaaat aaaaaggaga atgattaaca tgacaggatt
     4621 taacttagac ttttcaaaag cgcaacaagg gaacgaaatc aaagacggta cgtacgaagt
     4681 ggtcgtaaac aaagctgtag aaaatgcaac taaatcaggc gcagaattta tcgatatcga
     4741 tttgatcgtt cgtaacgatg tggatcaacc gttccaaaat aaacacattt tcgcaaaaat
     4801 ctggaaagcc aaagctaccg gcaaatataa cgaaggtatg atcatggcaa ttgcacaagc
     4861 gctacagttg gaagacggaa aatcatataa cgggtttgat gaattgctgg cggactttgt
     4921 tttgaaaacc gcctcagtac gcgtgaaaac tgaagagtca aatggatata aaaacgtcaa
     4981 tgtgaagtct tgggataaaa cgaacacacg gggcgtaatg aatcaccaat tcaaaaatgg
     5041 tgatgaacca tccttcggac cggaacgttc aagagcgaca acggttagaa atgacaatct
     5101 tccattttaa ttgaggtgta atcaatgtat gaaaaaattc caaaagagct taaaaatctc
     5161 aaacaatggt gtgtttataa gctagtttgg gatgaaaagc ggcagaagca cacgaagatc
     5221 ccttataacg ccaacaacgg gcataaagcc aaatccaatg acgagagtac ctggtctgat
     5281 tttcagaccg ctctcgctgc tattgatcac tacggaatga gtggactggg gttctttttc
     5341 aaaccacctt atttcggcat tgatatcgat aatgctgaag gtgaagttga gcgatttaaa
     5401 tcaggtgata tagaagaaaa tattatctat gaatttatcg aaagcatgag atcctatgct
     5461 gagtattctc aatcaggtac tggcatccac attattgctc gcggagaatt gcccggcgga
     5521 agacgcagga aaggtgacgt agaaatgtac cagaacggcc gattcttcgt tatgactgga
     5581 aatgccgcat caaaatattt agaaataact gagccgaatc caaaggatat caaacgccta
     5641 tatgatcgct acgttggaga tgaaaaaatc atccaattca aagaagaaaa tccgctgatg
     5701 aataccgttg atttaccggt tgaagaaatt atcaaacgtg ctgaatcgtc ttcccaaggg
     5761 gcaagattca aagtctttat gaacggcggt tgggaagctt tttattcctc gcaatccgaa
     5821 gcggatatgg cgttcgccaa cgacttggct ttttggaccg gtagagactt tgaaaaaatg
     5881 gatgagattt tcagagcgtc tgcaatgatc cgtagcaaat atgaccaaaa aaggggatcg
     5941 gcaacttacg gagaatctct actaaataag gcaattgctg atacaaattc cgtctacaac
     6001 cccaaacgga aatctgattt taagattttt atcaaagacc aggaacagcc aaaagaggaa
     6061 aaatactatt cgtacgacga caccggcaac gcggatcggt ttactgatat ctacggaacg
     6121 ttagtcaaat attcgtacat tgataaatct tggtattact acgatgggaa agtttggcta
     6181 caggacaata ccggcgaagt tcggaaaatg atcgatacga cagtagacat catgggaaaa
     6241 gaaccgttaa cgatccctga aggcgcagac gatgaaacca aagaagcttt gatgaaagcg
     6301 aaagaaaaac acgtcaagcg ttctcgcagc aatgccggaa aaaacgctat gatggacgaa
     6361 ttgaaacatc gactatccgt attgccggaa gagttcgaca aagataaaac gctgttcaac
     6421 acacaaagcg gctatctgtc tttgcatgac ggtcttctac acgagcatga gatagacaaa
     6481 atgtttacac gtgtttcaaa tgtcgagtac acagaatcgg tcgattgtcc tatgtgggaa
     6541 gaatttatta atcagatttt tgacaatgat caagaattaa tcagatatat ccagaaatgt
     6601 gtcggatatt ccctgaccgg atctaccaga gagcaatgta tgttcatcct ctacggtcat
     6661 gggtcaaatg gaaagtcggt atttttggaa attatttctg aattgatggg aaactatgcg
     6721 atgactatgc aggcacaaac gatcatggtc aagcaaagcc aatcatcagc aaacagtgat
     6781 attgcacgat tgaaaggtgc gcggttggtc acttcgtcag aaccgaatga aggcgtccga
     6841 ctggacgaag gtctggtcaa acaattgact ggtggtgata aagtcacggc tcggcacctt
     6901 tatggaaaag aattcgagtt cgaacctgaa ttcaagttgt ggctggcaac gaaccacaag
     6961 cccatcatcc gtgggaccga tgacggcatc tggcgacggt taaatttaat tccattcact
     7021 gtccaaatcc cggatcacaa aaaggataaa aatctgaaat ttaaattgca gacggagttg
     7081 caagggatcc ttaaatgggc aatcgatggt tgcctgttgt ggcagagaga gggcttggag
     7141 aaaccaaagt cggttgttgc agcgagtcag gattatcgaa acgaaatgga tcaaataggt
     7201 acatttatcg aaacgtgttg cgaaacaggt ccaggactaa aaatatcagg cggagaatta
     7261 tacaaagttt atcgtgaatg ggcatcagat aatggcgagc atactttcac taacacgaag
     7321 tttggcagag agatttcaaa gaaatatagc aaagaaaaag ctggtggatt tatggtttat
     7381 aaaggaatca cattaaaacc aagaaaatac gataacgtta gagaactttt taaataaatc
     7441 tggagggttc tggagggttg agaacgaaac cctccaaccc ttagaaacgt tgatatatca
     7501 gtatttatat tatatttttc tttctttctg gagggtttta aaaaaataat aaagtattaa
     7561 agtaaaaaat aaaaagtaaa ttatataaaa aagaataggt ttttgggtct gaccctccaa
     7621 actctccacc aaaatcttcc taaatgcttt gataccaacg attaaacagt ctggagggtt
     7681 acattgaaat cagaacatca aatacaagac gagatcagaa ttgccttatc aaaacatggt
     7741 tgcacgattt ttcgatccaa tgcgggacaa gtgacaacga tcgaagggcg gcaattttac
     7801 gggatgccaa aagggttccc agatctatgt ggacatcgta attctgatgg gaaagcaatt
     7861 tatatagaag taaaaaatga acgcggtaag ttgcgtgaag atcaaaaaag gtttgctgaa
     7921 tttttaaacg gtcaacctgt actatacggt gttgcacgat cagtggaaga agcattgaaa
     7981 attgtggagg aatcatcatg acaacagaag aagtgattca aatgcgtatt cgaagccttc
     8041 agcgtgagat tgacgatctg gaacggacaa aggcagtgat ggtcaatgaa acggcgagga
     8101 aggcaatcga tttgcacata gagaatttaa gaagggaaat ccatcgattg gaggaatgag
     8161 cgtggataag aaagcagcaa tgaaacgaat catcgaactg acacattctg agaattggca
     8221 agaagacaaa gaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggagtgaaaa
     8281 aaccaaacgg agaacaccga gaaaaattgc aatctggcat ggtgaccgaa ttctagtaac
     8341 aggtactgct gaacagttat ctgaaattac tggattaagc aaaaacatta tctgggatag
     8401 agctaaacgc gagaacgttg attcgaaagg tcgtcaattt aaacattggg agaagaaata
     8461 atggaggaac taatcacaaa agtagagcag tgggcaaaag ataagggatt ggatcaagct
     8521 gattccagca agcaaatgtt aaaaacgatc gaagagattg gggaagttgc cgcttctcta
     8581 gctagaaaag atgaatatgg tttaagagac ggaattggag acgtagtagt aaccttgatt
     8641 attttagcta tgcaaaatga tatggatttg tacgagtgtc tgaaccaagc atacaacgaa
     8701 atcaaaggac gcacaggaaa aatggtagat ggtgtattcg tgaagtcgag tgatttggag
     8761 gacagcgatg aataaaaaag tattaattga taaacaagca ttgattgatg aattaatgaa
     8821 aatacctggt gtgggatcta atagtgacgc tttagaaacg attaaacgtt tcccatcgta
     8881 tgaaccgcag aagccagttg tgcctaagtt tgtggcggaa ctactcgact attatcgtca
     8941 gtcaacagat gttgatttat tagctttatt gataactttc catgattggt attatcgcaa
     9001 aactaaagac ggtgagcatg aagaagcaat tgattggcta gttgatcatc ctgaaattta
     9061 tatgcgtgca tggctgtttg gctacgagct cgagaaagag ccgttgtatt gcgtaataat
     9121 caatggtaga tatcttgtca aagtattcag caatacaaac gttgtcatat tagttccagc
     9181 agatgaattt tcacagtacg ttactcaaaa atttcaatta actgaaaaac aaatcaaagc
     9241 aattgatgaa agatactggc cgtttgctgt gccagtggat gaggtggtag aaggatgagc
     9301 gacccataca ttactaaatt agaaaagttg tccgaagatt ttgagaagaa taaaaataaa
     9361 ctaactaaag ataaacttat agaactggtt aggttggtta tcagaggagc tattacaaga
     9421 gaaaaggcgc agcaaaacat agaaattttt gctcgtagtg tccgagatgg attagagaag
     9481 aaagttaaac aaaatgaaca acagacaccg cagaataaca aaactaagaa aacgggaact
     9541 gaatgtacta aagacaaagt ttgaaaaaga atatggaatt tcagcagaag aaacatataa
     9601 agtggcaagt cagtgtgttg ctgatgcgag cggcgctatt cgtaagtttg gaatttcgat
     9661 attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg attttactcc
     9721 agcagtcatg gtatcggcgg tttaatgcta gatatgccga caaagaaccc taaaacacgt
     9781 gagaaaccaa aattcaaagt cggtgacatg gttcgctgtg aagcagaagg attcatctat
     9841 ccattttgtg gatatgtaga acatctctat aatcactcag caatcattcg tattgaaaac
     9901 acgatggaat gtgacaagtg gttagcgaaa agcaaagaga atttagcagt agcgagattg
     9961 gtggatatgg aggttatata aacagcgaaa aaatgtactt ctaagagaaa aatttatttt
    10021 ttataaaaat aggaaagtat cattatgaaa aagaccacag agataaataa acctaccaca
    10081 taaaaaatga atatttcttg cgattcaata taggtatatt taacaatttg tacaataaaa
    10141 aatactatag gtaaaaaaga taacatgagg agcttatttg ttctagagtg tttgacaaag
    10201 taacaaaatg cgacgatacc aatacaaacg ggaatccaaa ataacagcat gctccacata
    10261 gtcactcaac catctcctat acgctttatt atcaatagat aatatcattt ttcgtaataa
    10321 agataaaaaa ataagtaagg cttctcatta aaatataaaa aagacagccg accactgact
    10381 gcctatataa gagtattgaa ataaaaagct gctgatataa taaattccac aagtttatta
    10441 tatcacataa aggagcggtt tgacttgatg caattgttac gagaggtaga tttcaaacag
    10501 acaagatgta atgcgagaga tgtgctgaag aactttcggc gtttggagcg gatggcaggt
    10561 cgctctttga tagatattaa gtcgccgatt attacggata tgccgaaggc gccgaagcac
    10621 ggcaataagg cagaagacgc aatcattcag atgatggata tagaagcgga gagagacgcg
    10681 attctagcag ccttgatggc tcttagtctg attagtcgtc agatactcta ctatagcttt
    10741 tgtgtgccag atagcttctc aaactacaga attagccgtg aagtgggtta ttcagaaaga
    10801 agtatacaac ggatgaagtc ggaagctcta atagagtttg cagaagcata taaacacgga
    10861 agaataattg cttataaata atttggcggc tttttggcgg aatgatggcg gtttttagct
    10921 atttaccagt gatattatgg tagtgtcgaa agattagtga taggtctaag acaaaataat
    10981 aataaaagga acatcgtttt attattgttt cacaattaag cttcgataga cagcaacgga
    11041 aatattaaga ataaggatgt gaattttaac tccttctaaa ttgttcttat tatctatcat
    11101 ccgttgctgt ctattgtttt tttaattatt cacacgataa ctaaaggtga gcgaagagaa
    11161 atgattccat taataatttc aatttttgcg ctttgtctta atgtctatat gattggattt
    11221 aaaaatgggc aaaataaaag atagtagtaa ctaagaaaga aatttttata ttgtcactgt
    11281 ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa ctatgcaatg
    11341 ttcgattcat tgccagtgac tttggcaacc gaggcatcgg cggtttaaaa atataggggt
    11401 gcgcaatttc gtacgcgttt tgtgcatcgt gtaggttgct attacatatt agatcactcg
    11461 ttgagtggtc tttttatttt acataaagga ggtaacaacg atgtatagac cacaatactt
    11521 agaacagaag tatgaagtaa tcactgtgca aaacggtaac ggtgagatag tacgaaagta
    11581 tagaagacca ataaagagcg atacatataa acgaaaggaa aacaatgaag ttatttcttt
    11641 tcgcagaagg agaaaagcca aatgagaaac tactggtatg tatcgctaac taatgaatat
    11701 cctcgaacca ttgatgattg ttcagtgcgt gttgtgcgtt ctgtacaaat caaagggaag
    11761 tactctattg tcgaaatgct aagagaagct acaccaaacg aagtggataa atgcaagctg
    11821 atatattgcg gtcatggcta ttggaaagac gagtatatcc aacaaaacat tgagaggtgg
    11881 atagatagat gagttacctc gaacatttga aacgttgcta catgcattct aagaataaac
    11941 ttcctgacag ctacactgta gatgatgttg ctatacatgt attgaagact gagagccata
    12001 gcagtcctga tggtagcagt aaggagcaga cgctcgcgtg gttcaagttc tttaagtgga
    12061 ttaaggagga agagtaatgc acatgcacat aggagaaaag atatataaat tgagacggct
    12121 taataacgaa acaatgaacg acttagttaa acaaactgga gtgtcaaaat catggttatc
    12181 tgatattgag tctggaaaaa agcaacgcgt tgattttaat aagatatgtg caatagccaa
    12241 acattataaa gttagcttag aatactttcg ggatgattat ttggatagtg atgaacacga
    12301 ggatattatt cctttgacag gcgaaacaag agttaaatca gtgaaatcgc ttgaacaaag
    12361 cctaaagtat ctgaataaca aacaagaacc taaccttcta gagatcaaac taaaagatac
    12421 tgactcagta ccagaggttt ggtacaaggg tgagaggttg gatgaattgc ctaaaggatt
    12481 agtagatgtc tcgtatcatt ggaagactga tgattttact aatgatgata gaggagcgaa
    12541 cgacatcacg attcaatact tttctagctt taatgataag tatccagata agaaaacaat
    12601 cggacacaag agagatatgt aaatgaaaga agttagacct agagacgaaa tagacaaact
    12661 atacaagacc aaacgatggc gagacctaag gcaagtagta atagctaggg acttcggcat
    12721 gtgccaagag tgcaagcgtc gagggcggaa cacaaggggc acgatcatcc atcacatagt
    12781 cgaggcgagg gaagacctgt cactgttctg gtccgtagat aaccttgaat gtatctgtgt
    12841 agcttgtcac aacagagagc atccagagag atcaggcggg aagaagaaac caaaacctaa
    12901 atcacatatc gttaaaatgt attcaactcc tgaaagataa gtttgcagcg aaatgaaggt
    12961 agccccccta ctctaaaaga ttaaagagta aggcttgaga agaacggtgc tgtccttcct
    13021 tcgtaaaaag accgcttttc aagttttttg gagaaaaagg aaaaagccga ccaatttaag
    13081 ccggctttgg acgaagctat ttcttagtcc atttgtttcc tttttgagaa gtagggggta
    13141 atcggtcgcc tggatcaatt tttacttctt gtccgccttg gacatttcca ccacgaggac
    13201 ccacttcttt gtaggttcct tttggtttgt tgtcttcgcc gggtttatag agttctccca
    13261 taggaatccc tccttaacaa atttcggcac agcactgccg ataacctaat tataaggatt
    13321 gtgataacga tttcaatcta tcttttgaaa gaaggtgata ttatgccgca accagcgaag
    13381 agtgcaaaat tacaattatt aaacggaaac ccaaataaga agaataccga agaactccgc
    13441 aagcgagcgg ccgcagaaga caaattaaaa atggctactg acaaaatcaa accgccgtta
    13501 tggctagatt cgctaggaaa ggataccttt gagtttatcg ccgatgaatt gctgtctgtg
    13561 gatttaatca gtaatccgga cgtccataca atggctctat actccaattg gtattcgcaa
    13621 tacgtttctt tagaaaaaca gcttcgaaaa ctacaacgag agtacaagtt gaactatgcg
    13681 cttgcgaaaa aggaggcaga ggcgagaggt gagccgttta atgaacctaa tgaattaatt
    13741 ggtaatccgc tctctcggca gatggataca gcgtctcgga atctccgttc ttttggcgct
    13801 gatttaggac tatcaccagc agccagagct aagttagcta ttaagatggc tgatgatggt
    13861 ggtgatgacg atgacgactt ctaatatttt ggatatgtct tacacagaac gtgtggacta
    13921 ttggcaaagc tatcttgagg agcaagcttc ttggggtggc tttttaaaat gtccatatcc
    13981 ggaattgtta actacttggt atgcggaacg attaatcgat ggaagcatac cagccagcaa
    14041 agaaaatatt caagctgcta aacggcatat gcgtgatttg cagcgccaag gaacagatga
    14101 ttttccttgg atctttgacg aagaaaaagg tcaccggcct attagatata tcgaaaaaaa
    14161 atgtaaacca actgaaggcg actttggttc gtttgttttg caaccttggc agcatttcat
    14221 aattggatcc atgtacggat gggtacatcg tgatacagga gagcgtcgct tccgcgaggc
    14281 tcttattttt gttggacgta aaaacggtaa gttttgccgt ttaaaaatcg ggcaaaatcg
    14341 gtgaaaacct ttatttttgg ctttctttta gtaataattc gatagcttta tcaagcaatt
    14401 tagacatagg aaccatagtt tcttccgaca ttttttttaa tccttcgtat aaatctttat
    14461 caattgcgtt agaaattcta attctgtttt ttagtccgta tttgtccatg tgtgacactc
    14521 ctttttattt tactataaca tccgattcca ccgcttgcaa ctaccatcaa ttgatggtag
    14581 tatataaaag aggtgagtag attgaaaaaa tacattattt acaaacactt aaataaaata
    14641 aatggcaaaa tttatatcgg tgtcacaaat gatattggca gaaggtggag gagcggtggg
    14701 attgaatata aaccaccaaa gaacgaaacc caacaccaca gaagtttttg gaatgcaata
    14761 caaaaatacg ggtgggaaaa ttttgatcat ttgattattg aagaagactt aacgatgaaa
    14821 gaagcttttg agaaagaaaa gttttatatt gaactttatg actcaactaa taagaaaaaa
    14881 ggttataaca tcgcaaaagg cggaaatggc ggaataatat ataaagttca ccccaaagga
    14941 atgctaggga aaaaacaatc taaagaattt tcttcaaatc acagtaaatg ggcaaagaac
    15001 cataagaata attgtatgac aaacggtgat gttgtatggg gtgttactca cgaacaccca
    15061 aaaggcatgt tgggaaagca ccacagcaaa gaatcaatta tgaaaaagaa agcatatagt
    15121 ggtgaaaatg ctgtaacctc aaaaccaatt gtcgcaattg aacgggatgg aactaaaaga
    15181 gaatttcaca gcgctaagct ttgcatggct tactattcga ttagtacatg tgttttttac
    15241 cgacttttaa aagatggcgc cccttatgtg ataaatccta aagcgaacta cagaaataaa
    15301 gaaaaaatat tagctatcga aggaattatg ttcaaacata aagaagatac cgaggtaagt
    15361 tagtagattg cgaaaggtta ctaactaccg taacgcgtag gagttgaata aatataatgc
    15421 tcccaagagt gtccgacagc agataatttg ctgaaaatgt acgctaaact gggccagaac
    15481 tgactgaccg atgaaaatga gggtgacctc cagagtgcga gataaaaagc tcgcagataa
    15541 taacaatcga aaacatcact aatttcaggc ctttccacat acatggtcgc ttatgatgat
    15601 gaacaaggcg ccaacgttta cgtattggca aatgctcgtg atcaagcaag cttgttgttt
    15661 gataaggccg cagaaatggt caaacaatcg ccggcgctct ttaagaaatt tggtaagcct
    15721 aaacgatcaa gtattaatta tgctcccgcc ttttctaaaa tggaaccacg cgcctcagat
    15781 agccggaaat tggatgggct aaacactcac tttggtattt ttgacgagat ccacgagttt
    15841 acgaattaca atctgatcaa cgttatcaag aaatcaagag gaaccagaaa acagcctctg
    15901 atagtttata tcacaactgc tggatatgta ttagatggtc cgttgatgtc ttattttgag
    15961 caaggtgtgg actgtttgga acatttggaa gatgacatcg atgaacggac tttctattat
    16021 ctggcaaaac ttgacagtgc ggaagaggct gatgacccaa gattatggat caaagccaat
    16081 ccgaatattt gtctaatgaa ttttgttggc atgctagatg actatgttaa ggataaaaaa
    16141 gatccaaaag aatatgctga ctggattacc aagcaattta acttgttttc cgatatcgat
    16201 gagctgtcat ttgtcgatat gcctaccatt aaacgaaaca ataaaaccat cgatattgaa
    16261 acgctcaaag gtaagaagtg tgtcggtggt tttgacttgt ccgaaacgga agactttacc
    16321 gcagccgttt tagaatttcc gcttgaaaca ggcgaggtat tcattttgca acacacatgg
    16381 atcccacaag ctagatttga tcgagataac aatcaagagc gtatcaaagc gtgggagaag
    16441 gtgggagatc taacgattat tcctggtgat tacgtcaatt atgaatacgt cttaaattgg
    16501 tttgtagaaa attcgaaaat ctatgacatt gtaaaaatca attatgacaa ggccaaggcg
    16561 ctacgattaa ataaagaact agaaaatgca ggatttgaaa ccaacgagat tcggcaaggg
    16621 tttctatcat taggtgggcc aatgcaaaac ttcaaggaaa tgctattgga cggtaaggtg
    16681 attttcaaca attccaagct ttaccgatgg tatctatcca acgtcaagct ggtgatggat
    16741 cgcaactcaa actggatgcc gtctaagcag tccaagagta gaaaaataga tggttttgca
    16801 gcaagtttga acagccacgc cgaagtgttg aatatgttgg ttaatcctgt cggaaccggg
    16861 aaagtaacct attactcgat ttccgattta atgaatatgt aagaaaggtg tggaggaatg
    16921 agtattttag atcgtttgcg ttcttttggc cgagcgaagc cgaaagcgag caaacaagag
    16981 tattttttga atgacccggg attgataccg tatttagtcg gaaaagatga aatatcagaa
    17041 gggatttttt ccgtaattag ccgtgtatcg aacgtttttg cgtctctccc tctcaaaatg
    17101 atagatgtgg agtttggcca accggacgac tgtcctgcat acaacttgtt gagcgaaggc
    17161 cctcgatatt ttacaaagtt tgattttttc cgggacgtgg aagttttgag aaactaccaa
    17221 gggaatgcgt atgtgcagat tttccgaaat atcaatggag aagtagcaga tatggcgtta
    17281 gtaaaacctg gtgcttgcca tccagtgatt gatatggata gcggggagct ttactaccaa
    17341 gtaactgcga ctgacaaagg cagttacaag caagttatct atgtacatta catggaaatg
    17401 cttcacttta aacaaccgag gtttggcggc ttggaaggta cagaccccac aaaagtatta
    17461 acgaataccc tcggatatga tcgagaagtc cgaaaaatct ctttaagtca gcttaaagga
    17521 agtaatgaag ggctaaaagt taagtttgct agcaatatgg atgaagaagc taaaaaagct
    17581 acagttaaaa acattgctga tttttatcga caaaacggtg gactacttgt ggaagaaaac
    17641 ggtgtagaaa tcgaacgttt acaacgagag ctggtagaca gcaagctttt agatactgat
    17701 aaagtatctc gctccagaat cgctatggtc tacaatgtgc cggagcattt catcgggaat
    17761 aaccagtcga gttactcctc tcaggaacag ctcaatatgg aatttttgac gtacaatcta
    17821 gtaccgaccg ttaatcaata tgaagcggaa ctaaataaga aaacactatc gagaaccgaa
    17881 aaagcaaaag gttatcgata taagtttaat gtcgcaagtt tgctaagagc tgacactcag
    17941 gcaaggggac aattctatca aatcatgcga cgaggcggag catactcagc gaatgatgtc
    18001 cgaagatttg aggacttgca accgataaat aaaaccggta tggatgatta ccatatttcc
    18061 ggagacctat atccaatcga tatggatcca acattaagaa aaacaacctc gtctaaaagc
    18121 gtagccgaaa acggttaggt ttttttagtt tgcaccgaag ggaggtggaa aaatgaaaaa
    18181 agtgacgtta agcggcgatg tcgtggataa cgataccgcg tggctttatg actggtttgg
    18241 gatcaattgt atctcaccag ggaaaatttc tgccgctctt acagaagcag cgggggatga
    18301 agtagaactt gatatctcat cgaacggtgg ggatgtccta gcggcaagcg aaatatatac
    18361 cgctatccgt gcctatccgg gaaaggtatc cggaaatgtt gtgagtattg cagcaagcgc
    18421 tgcaagtgta atcgcttgcg cttgcgaacc gcttagaatc tcacctacgg cacacatcat
    18481 gattcataac gcatgggtga ccactagtgg caacgctgag gaattaaaag ccaatgcaga
    18541 aatgttaagc agtgtggatg agtctattgt taatgcttac gagatcaaaa caggactcga
    18601 tcggaaaaaa cttgctgatt taatggcgaa agatacttgg ttaaatgctc aaacagcagt
    18661 agcagaaggg tttgcagatg aaattatgtt tgcagaagca ccagtaacgg tactcaatgc
    18721 ctctcaaccg gttattccaa aaaacgcagt aactaagttg aaaaatttaa tactcaaagc
    18781 ggaagcaccg caagaagaaa cactcttaca gaaaaaacta aaagccttaa atggagggaa
    18841 aaacgaatga atttagaaca attaaaaaat gcgtgggtcg aggcgggaag taaagtctct
    18901 gacttaaatg cacaactcaa tgcagcattg gttgacgatg aaaaaacaga agaagatgta
    18961 gtaagtttgc aagcacaagt aaaagcagca cgggctaaac gggacggatt gaaagagcaa
    19021 gtggcaaata tggaagccga acaagtctta aacgtcaaaa aagaaccatt agataaaaaa
    19081 gatgaaaact tgaaaaacaa gtttatcaaa gactttaaag cgatggtcaa tggtgatcct
    19141 gctattatgg ctactttgac atctaatacg gatgaatctg gtaatgctat cggattgact
    19201 attcctgtag atgtgcaaac gactattcat actttggttc gtcggtttga ctctttgcaa
    19261 gaatacgtaa acgttgaaaa agtgaccact accagcggtt ctcgcgttta tgaaaaatgg
    19321 tctgatatta caccgttgac tgctttggat actgaagacg gtgaaatccc agcaaatgat
    19381 gatcctgcac tttacttgat caaatacttg atcaaacgct atgcaggtat ttccacagta
    19441 actaacagct tgctaaaaga tactgccgaa aacattttgg catggttgtc taaatggatc
    19501 gcgaaaaaag tagttgttac tcgcaataca aaaatcttgg cagctattga tggaatcaaa
    19561 gcggcacaaa agaaagatgt tacagatgtt gatggaatta aagatatcgt aaacgtccaa
    19621 cttgatccag ctatcgaagc tacatctatg tttattacaa accaagatgg cttcaatgtt
    19681 ttagataaag tgaaacgtgc tgatggatct tacttgttac aaaaagacgt agcttctgca
    19741 actggatata ccttcttagg taaaccaatc aagaaaattg cttctcgttt cttgccaaac
    19801 aaaggtacgc aagctactcc taaatatcca ctgtacattg gtgatctgaa agaagccgtt
    19861 acattgtatg atcgcgaaaa catgagcttg ctgacaacga atattggtgg tggagctttt
    19921 gaaacagaca ccactaaagt acgcgtcatc gatcgcttcg atgtgcaact agttgatgat
    19981 gaagcggttg ttttggctac ttttacaact attgcgaacg agacaccggc gaaagtttaa
    20041 ggagctgatt ccttatgatt cttgatccta aaatggattt aggcgaaatc aaaaacgcac
    20101 taaagattga taccgatgat gacgatgtgg aagtaagccg tgcggcacaa gctgcaattg
    20161 catacattag aggggctatc gggaatgata agccctcttt ttataagcaa gaaaatgaaa
    20221 cggttgatct gattaattta gctatcctgc aattagcaga tcattattac aacgctcgat
    20281 cagcaaccgt gagtgggaac ttgcgagagt acgatttagg ttttacaagc ctaatcttgc
    20341 aactcaaagc aagttatttg ctttttgtgg aggaggagta gcgtatgccc cttatccaaa
    20401 caggaaattt aaatcaacgc atcaagtttg tccgagatac gacggttaag gatgaggacg
    20461 ggcaagttgt cccgacttct acaaccattc ttacttgctg ggcaagtgtg cagacacaac
    20521 gcctgaacga tattaagacg tcgattggta cggctttgga aggaacactg acgttcatta
    20581 tccgctacca acaaaaatca gagctgacca atgatatgaa agtgcgttgg aatggaaaaa
    20641 cgtttgaaat cattacgatt acgaaaggcg agtttgcgaa ggacttcacg acaatcattg
    20701 caaaagaggt ttcaaaatga gtgtagaagt cgatgcaacc gaagtgtaca aagcgcttag
    20761 ggaagtaaaa gcaaacgttc aacgagtgga aagcccagca cttagaaaag ctggggagta
    20821 cgctcaagaa gagttacgac aaaacacacc ttactgggat ggaacgaagt caaacggtaa
    20881 acgtggttcg tatatgcaag aacatgctaa gaaccatgtg gttacaagct cgataaaaaa
    20941 cggattggta gaagtcggct atgacaaaga tgtttcttgg cggatgcact ttatcgagtt
    21001 cggaacaatc aaacaacgtc caaaaggttt cgtacaaaaa acacaaaagc aaatcgaaaa
    21061 acaagtaaca caaatcattg ctgacgaagt aaaaaggagg ctaggacttt gaaaacggca
    21121 gtatcacaag tctattcaat tctgaatagc aatgaaaaaa caaagaacat tgatttttac
    21181 accaatagtg ttccggaatc agctcaaaca gtacctagcc ttccagttgg cagaattaca
    21241 gagatatccg gcaactatga agattttgca agcaacaatc ctttgaccat tcaatttaac
    21301 gtacaggtag atgtatgggt gtcaaccatg aaagaggttg atgcctttta ttttgccctt
    21361 gatgaggtta tgagggggaa tggttggcaa tgcgcataca cggaacaaac agatgacgag
    21421 gacttggaag gtgcaaagcg gattatcaaa cgatatgtag caaatatttc actaaattaa
    21481 aaggagagaa aatagatggc aacagtagga ttcgagagcg tcatttttgg cgtaaaaaca
    21541 ggtgcaggcg gcactctaaa agaattagta gcagataagt cgaaaggcgg agcgatcgaa
    21601 gctaaaatta ctggattagg cgcaacttct aacacaacat acgcttcaaa cgtaccgttc
    21661 ttcattgcaa gtaaaggggt ttcgtcgcca aaagttacgc ttgacgtggc agacttaatg
    21721 gataacggca tttacagcga aatcattggt gctaaaaccg tggatggtgc aaatgtaatt
    21781 ggttcagaaa ctgaagcgcc ttacgtgtcg gtagtcatgg ttacagcgaa caaagaagga
    21841 aaacgcttgt tcatgggatt gacaaaagga aaattcagtc atccagatat cgacatgaaa
    21901 acagctgaag acaaaggggt agaattgcaa accgattcca tcgaagggga attcatttct
    21961 gatgaacgtg gctatgtata cttaacagcc gtagaatcag aaggaatgac cttacaaaaa
    22021 ttcaaggact tggtaaataa caaagagggg gagtagttaa ccctgcatct acaccaacga
    22081 cagatacagg gacaccaaaa gaaccagaac caaaaattga tacacaaggt tagccatttt
    22141 tggctagcct tattttttgt aaaaacaagg aggaaaacaa atgattgaat tgcaattgaa
    22201 acttgacgga aagaaaaaaa cattcaaaca acaagatatt tccgcacgtg caatgcgtga
    22261 gtgtatcaaa ttttacgaga aagcggaaaa agcagaccta actgatttag aagcgattga
    22321 ttcaatgatt gcaattacag cagatatttt ccaagatcca gcagttacat ttgatgctat
    22381 tttagacggt ttgactgcga gcgagttagt accggcatta gaaagtgttt ttgaacaaat
    22441 aaatgaactg ggaaacaatg aaaaaaagca gacggcgagc aaaaagaaat aagtttttct
    22501 gaagctagga aagcaatgga tcaaatctac aaagatttaa tcgaagcagg ttggacgatg
    22561 agagatgtgg acgaagccga ctatcattat ttgttacacc tttttggaga agtggagagt
    22621 ggcgaagaat atgtagatgg tgctgatttc atcaaacaat ttttatcggc tgaagactta
    22681 gtaaaacttg aggaaggagg taaataatgg caggaaaagg acaaccggca ggaaatatca
    22741 agctagggat tagtttagat agcactagtt ttggtaacac gctggacgaa atcaatgcga
    22801 aagtcaaaca agctgagtcg aatatgcgtg ccaatctaaa ggcttatgat tcagcaggac
    22861 gttcatacga agcacttagt caaaagacga aagacttgtc tacggttatg gaagggcaaa
    22921 acgccaaagt aagagaatta acaaagcgcc gtgatgaagc gattagcaag tatggcgagg
    22981 aatcgaaaca agttgctaac cttaacacac agataaacaa tgctaccgca aaatataatg
    23041 cttacagtcg ccagttgaac gacacaaaaa aagaattggt gtattccaaa acagccgtca
    23101 atgatttatc taatgaaatc aaagaaaatg aacgacaaat gaacgccgaa gtaaaagcgt
    23161 tgaaagccgc tggtgatgaa tctggtgctt ttgaagcaaa acaaaaaggg ctagccaaac
    23221 aaacggaatt atccgagaaa gctatcgaag aacagcgcaa agttgtgaaa ctgatggctg
    23281 atgagtttgg cgattcagca aatgaaaccg aagatgcaaa aagggcatta gaaaagttag
    23341 aacgacaaag ccaaatatct agcaggcaat tagaagcact caaaagctcc agcgatcaat
    23401 caggaaaaga aatagaagat tttggcgaca agtccacaag gtcagctagg aaactggacg
    23461 gactaaaaga caaattaagc tcgctaaaaa gcgcattttc gtttggtgca gttgctggat
    23521 tagcgcataa cgctattagc agtgtagtaa gtggcgtgca aggtttggtt ggcgaagcag
    23581 taaacgcatc ggattcattg atgaagtttt ccaaaaccat ggagtttgct aactttggga
    23641 agtcacagat agaaagctcg aaaaaagaaa tgaaagacta cgccgataag acggtttatg
    23701 gtttagaaga aattctgaac acaaccgcac aattggcatc taatgggatt cctaactata
    23761 cagaactaac caaggcggca ggtaacttga atgccgttgc aggcggttct agtgatacat
    23821 tcaaatccgt tgccatgatg ctaacgcaga cggcaggagc tgggaaacta acaactgaaa
    23881 actggaatca attagcagat gcgataccgg gtgcttcagg attgttacaa gacgctatgt
    23941 tgaaaaacgg agcttataca ggaaacttcc gtgatgcaat ggcgcaaggt caaatcactt
    24001 ccgacgagtt caaccaagca attgtacagt taggtatgaa tgacggagca gttaaggcag
    24061 ccacttctac agacacattg agcggttctt gggagcagat gaaatccact gtaataaatg
    24121 ggctacaaag tattatagaa aaaataggcg ttgaaaatat cactggtttc atcaacagag
    24181 taacaaaagg gattgaaaat tctattccta aaattactca atttataggt tggttgagag
    24241 atattggaac gtggatcgtt gaaaatagag agccactaac atggattgtc ggaatcatag
    24301 gcggaattac attagcagta aaagcattga acgtagcaag tatgttgctg gcaattactg
    24361 gcggaacatt ggcagcccct tttgtggcga ttggtgtagc attaggcgca ctagcaggtg
    24421 ctttggtgat agcttataca aaatctgaaa catttagaaa tatagtcaat gcggctttta
    24481 cagctgtgaa aaacgtagtt atgagcgtta tcaataattt ggtggaatac tacaaaatgt
    24541 tgtggagcgt gttgcagtgg ctttgggaaa aaataaaaga atgggcttca tggattggca
    24601 ataaattcat tgaaatgaag aacagcgttg tgaatacggt tcagaaccta tggaatggcg
    24661 tgaaaaactt cttcagcaat ggcgttggag atacgtggaa taaggtagtc ggttgggtaa
    24721 aaaacatttt caacaaagca actgaattga agaacaacgt ttctgatgta atcggtaacc
    24781 tgtggaatgg tatcaaagac acattccgta gaggtatcga tacggtattc aattggtttt
    24841 cagaactacc aacgaagatg aagaatgcca ttattggcgg taaaaacgcc attgttgatg
    24901 cgttcaaaag tattttcaac gcagcactta aagcgatagg taaaccagtt aacgcaatca
    24961 tccatggagc ttcatgggta ctagaaaaat tgggcgcaga acctctacaa gaatgggatg
    25021 taccacaata cgctacaggt acaccagcag gtgggcaccc aatcaatggt ccaatgatgg
    25081 ttaatgatgg acgtggagca gaaacagtta tcacaccaga tggtagagcc ttcattccta
    25141 aaggacgcaa tgttgttcta aacgcaccaa aaggaacgca tgtcttgaca gcagaagaga
    25201 ccgcgcaact tcaaggttct aaagctccta agtatcgtta caaaaaaggt actaacttct
    25261 ttggtaacat gtgggatagt gtgaaaaagg ttgctggtaa tgtaggtcac acaattaaaa
    25321 acgtagtagg tgacgtgtgg gactttattt cagacccggg agcgttagct agaaaagtac
    25381 ttggggggtt agatgtatta ggtggattga caaagtatcc attagaagta ggtaaaggca
    25441 tcctatctaa agcaacaagt gcactgactg aaaaaatcac tgggttgttc tcatctggta
    25501 acttagatac ctccgtagga acaaatggcg tctataaata tttggctgat gttgctaagt
    25561 ctgtgatgaa gaaattccca ggatttgtgg caactagtgg gtatagacca ggtgaccctt
    25621 attcacatgg taaacgtaat gccattgata ttgcattacc aggcgtcaca ggaggctcac
    25681 ctagatacac agaagcagca aactacgctt ttgacaaatt cgcttccaaa attggttacg
    25741 taatcactaa tgggaaagtt cgtgaccgtt caggacaatc aggtcaacca gcaactggtg
    25801 catgggagcc atggcccgat ggagatcact atgatcatgt gcatttaaac ggtgtgaaag
    25861 acccacaaaa cactcaaatt tcaggagata gcgtgggagg cagtggggta gaaagatggc
    25921 gcaatgtagc aattagagcg ttgaaaatga ccggtcaata cagtactgca aacttaaatg
    25981 cattactaaa tcaaatgcgt acagagtcaa atggtaatcc taatgcagtt aacaattggg
    26041 atattaacgc caaaaatgga acaccatcaa aagggttgct ccaagtgatt gacccaacat
    26101 tcagacagta tgcaatgcca ggattcaaca gcaatatttt tgacccacta tctaacatct
    26161 tagcttcaat cagatacgca ctatcaagat atggctcact aacaaatgcc tatcgtggag
    26221 ttggttacgc aaacggtgga attgtaaacc aacatcaaat tgcggaaatc gcagaaggaa
    26281 acaagccaga aattattatt ccgttagata aggctaaacg atcaagagcg atgcaattat
    26341 tgtctatcgc aatggacaaa ctaggagttt ctcctaaaac ttatggaaat gcgacaactg
    26401 tttccagtga ttacgaaatc ctagaagcca ttgaaaaaca ggcggcaaca acgaatcagc
    26461 tgttatcgtt attgctggca tttttcaaag gaaatagccg aactgataga gatttagctt
    26521 tagatattca gaagatcttg gttaggagga tgtagatgcg aactgtatta ctgagaaata
    26581 gaataaacga agaaattgat ttgtccacag aagactattt tgctactggg ttgagtaata
    26641 tggggtttga agtaaaaaaa gaacatgtgg gacagtgggg gaatttcaga gaaagcagtg
    26701 agagcgttga aatatctgaa tttcagtcat ctgtaatcat ttctgtgcat gggtttcgag
    26761 aaaaagaact gtacaattca cttgtgcagt tcctatcgga aggtccgttt gaactggaat
    26821 ttgcttttga cggcgaaaca atggtaagaa gatgcagtct aaaatcttta agtaagactg
    26881 aaatcgatcc gaaaacatcg ttactaacag acactttaga actatatttc acatcaaact
    26941 ggtattccgt aaaacgcgaa aaattaattc agcgaccgaa tgtagtgaaa acacgtggta
    27001 aggtttttcc gtataaaaga tcttatatct acactcaaaa cttgtgggag aaaaaaggtg
    27061 tattcaaatt caataataat tctgtatacc taacgaatag caaggaacgg atgtccccgc
    27121 taaaaattcg tgtgattggg aagtgttcaa atccgtattg ggaagtaatc caaaactcac
    27181 aaatcatcgc aacggatgga tacttcatag atatgactga aacacagact ctagaagtat
    27241 caagcctttt tgaagataca acggcgattt taaaagatat tgcaggggta gaatcttctg
    27301 tctaccaaca acaggattat acaaaaacta attttgttca ggccccaact ggagaattca
    27361 gcattgtgtt tcatgttggg ggggcagacg tagagattga gttatacgag gagcgtgatc
    27421 tgttttgatt ttagcagtca cgctttttca tcgtgattta atgctttata acgaacattt
    27481 attctctaac agttttgaat ttggtgtgga cgagatcaac gaagaggcta gtagctttac
    27541 gatggataag tacgttcctg taaaaacagg cgattttcta ctagcaaaat atattcctag
    27601 tggaaaattt gcatattttg gtgtaattac gtcgcaggaa gacgaaaaaa tcagttgtaa
    27661 aagcttactt agtttagctg atagtgagat accgactgca cgtgtgtcag gagataacta
    27721 cgaagagcat atccggcggt tgattgaata ctatttgctg aatgatccaa cgaaacaact
    27781 aaaagatatt ttagacgtca aagcagaaag tgcgacctct cattcgtatc aagctaccga
    27841 tacgaataaa cacaagttaa gtgcgtatat tctcaatggt ttcaaaaaat acaacgtaaa
    27901 atggtatttc aagggaattc aaaacagaaa aatttataca ggcatacgtg ctgtaaatga
    27961 atcaatttac attaaagaca attcttctga atttagcgat tgggatgtgt ttgttcaggc
    28021 gccgggcgct ggaaacgaga acaagctatt aatcgttgat aaagcaatga aagatataga
    28081 gaaaccgata atactgtcaa catggtattt ggacgaagaa aacaatttga cacaagatgg
    28141 atcgaaagaa aatatcacga aaccaactgt caatttggtt aatatctacg atcaaaccgc
    28201 agaagataaa gcatcctacg aagatgtggc aaaatcagag ttgaaaggca atacgtattc
    28261 gcatgaaatc aaagtgaacg ttgtaagaaa tgcaaaaaat ttgaatgtcg aaacgattga
    28321 aacagggatg tttgccacga tttcttataa aggaaagata tacaagtcgg ttttgacagc
    28381 ttggcgaata tcaagtgata aggagtttgt ggaattgact ttcggaaaca taagaagtcg
    28441 ttttatggat tattttgaag ataatggggg ataaaaaatg gttagcaatg tggatggata
    28501 tcaatttgaa aacgtgaaag taagcgcaga aaatgatgct agactttatc acgttttata
    28561 taatcggaaa aatcaggtta ttgatggtta cgatcagtct atgaatttat cttcaagcgg
    28621 attaacagta aaagttgctg caggagcagc gattattcaa ggtcgtatgg tcgttgttcg
    28681 acaagaagaa agtataacag ttccagcaaa ctcaagtggt tatatagcat taacagtgga
    28741 tttgacacaa gaagttatac ctggatctat tcttccagaa tcggaagaat atgaatggac
    28801 taataatcaa gtcaagctag aatttataac aaaagttata aaaggtaatt taaacaatgg
    28861 tgataaggtc tataatttac cactatgctc agttaattct actggatcaa ctgtttcaat
    28921 ctcaaagata tcggatagtt acgagctgac tctctctaaa ggagaaattt tgtggagggg
    28981 gactgcgtta atgcatgata ctcaaactgt ccaaccttca aaaaaaattt ggcagacagt
    29041 ttcaggtttt ttgttagtat ggctcccata cgaaaacggg caagcaattg aggatagata
    29101 tgtaactacg cctttttata aggagcgtgt aacttttact aatgttttag gagaaattgt
    29161 ttcaggattt gatgactatc acaaaaagtg gtttagtaaa cgaataaact ataattcgaa
    29221 tactaatata tttacaggtg cagcaagcaa tgcaagtgga gataacgcaa atatggtgct
    29281 taggtatata gtttcttttt agttaggagg tatggaagag tggcaaattt agaaattaaa
    29341 ttatctgcaa ataagagaca gccttatcta cgtcaccgtg ttgttggtag agttggtgat
    29401 ggggggctca caacaatcaa tgtacaactt cttgaggaag atgaaattac accttttgta
    29461 attaatctaa acggtacttt gaaatttgtg ggcgaagttt caaacggtaa ctataccgag
    29521 ggagaaccag aaataatcga ttcgactaat gggttaatta gttacacgtt cactaagtca
    29581 aatttcagca cgagtcatca attcaaacaa gcatattttg aatatgtaga tcctaacggc
    29641 aaaaaagtaa cttttcagaa cttcatcata gacgtgttag aacgagtgga tattaattcg
    29701 gagcaagcga aatactatat ttcttcattg gaaaaattac agagtgaaat gcaaaccact
    29761 ttcaatcagt tcattagtga taaacaggtc caatacgatc aaatctactc gaaatataac
    29821 gaattagtaa gattgataaa tgaatcagat aagcaagtaa atgatcgtat tgatcaaacc
    29881 aatcagcaaa tcggcgatct cggcaagctg aaaaagatgt acagtaacag catcgacttt
    29941 gggggctatg attattcggg gaatccgaat ttgttaagta agctatcata cgacttaatt
    30001 gaaaatcaaa atacttcagc tggaacactt tctaagggtg aaaactcgtt taaatataat
    30061 aagatatcag ctgaagtgga aggtggagta gagttatatt ataaacgaag aggtatagct
    30121 aactggttac cctctaataa aacgcttgta atgactgtta agcttagagc tggagcggac
    30181 tatagtccag ttgacggaaa acttatactg attagatata ggtatgttga cagtggaact
    30241 ggcaagattg ttttagactt acctattaac agtaattcga taactcagga atggaaagag
    30301 tttagtatta ctggaactac tccaacattt agcccacaag cataccatcc ttggatacaa
    30361 tttagggctc aagatggggt acttggggaa atagaaatga gctatgacat caaaatcgaa
    30421 gaaggctcta cagctacacc atatcagcct aacttacttg atgactctta ctggctaggt
    30481 aaaacaccgt tgggtgagaa tttgataacg aatgccaagt tcccaattat aacacgaagc
    30541 aatcctattt ctggttttga tatttcagaa gaattaataa taggtgaaac ttatacggta
    30601 tctttaaaag ggactaagcc agctactaaa gaattccact tatactacgg agaagcaaat
    30661 taccagcagt tgcagtatca ggcaacttta cttcctgtgg aaggattaac agatgtgtgg
    30721 agtgccactt ttacagccag aaatactagt gaaacgacaa atttacttag agtagcgtta
    30781 tggcaaaaac ctaattctgc aacatatgat actgttcaaa ttgactggct caagattgaa
    30841 aaaggcgaca cacgcacacc aaatatcagt cagtttaaat actttggtga aggcttgaaa
    30901 gacagtaaca atccgaacga ctacagctgg gatgtcacac cagagtatac tgaaaaaagc
    30961 ttgaataata cggttagtct gactgaaccg caatccatcg aaggattgaa aaatttcgaa
    31021 gatggtattc aatcaaaagg aaaatctgta ttgacatcag acgacaacaa atatgaagtc
    31081 gtaaccttaa cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa
    31141 accgtcacta tttatttttt tgcgttgaat gggaaaagca gtggtggaaa tgattcaacg
    31201 atactaacaa ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg
    31261 atagaccgtt ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa
    31321 tggcgaagaa actcaagtta tggatcggat tatacctttg caattactta cacgatttag
    31381 aaaggcgtga atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca
    31441 aatcattgtg aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat
    31501 tccaaattgg aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa
    31561 tatggccgta ctggcggaga gtatgcagga aaagtattgg acgacatgct tgtatccgtt
    31621 ccttacacag atcagccacc gctcgaaggg gttcgttcag atggcgaacc gctaacgatt
    31681 gctgatcaga tgtttgatcc taaattgaac caatggatta ttttagcgaa cgcgttagat
    31741 cacaacgatt taaacaatct caaagcgatg tacgaggctc ttgaacatga aaacggcaac
    31801 ctaaaacagc taaatgccaa actcatgcta agcgatgtag cgattaaaca ggaaaatact
    31861 gcattgaaag aaaaagctga cagtttagca caaatcaatt caaaaatgat gcttgcttcg
    31921 ttacaaaata gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga
    31981 ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac
    32041 tgacgatcaa gttcgtctat tcgttccact atgtatcaca gatgaagaag cagatagaat
    32101 tattagcaaa gaagagagcg catcttaagt gatgcgtttt tttgttggaa agttggtgga
    32161 acatgaaaga agaagcgctc caagacgttg tggagagatt agtaagaatt gaaacaaaat
    32221 tagacaacta cgaatcactt agagaaaaag ctgatagtgc aaaagatttg gcagacaaag
    32281 cctactcagt agcgctaaac aacgcagaag atatcaaaga aatgaaagcc aataataaat
    32341 ggtcgtgggg ttacatgatc ggtttaggca ttacgatcat tggctatttc ttgactaaat
    32401 tgtaaaggag ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga
    32461 cggttttaac agtattacca gctgcatctg ttttagtagc cacgttagga aaagcgtatg
    32521 gatggaatgg aacagatatg acagtactca ctatcaatgc agtagcaaca tttttaggcg
    32581 ttatcactgg tgtgtctgca tataacttaa aaaaatagga ggaaaaaaat gaagaagaaa
    32641 atcattttat cattgagcct aataatggct ctttttttat tgccaattaa cgggttcgcc
    32701 tatacgatca acaatgaatt taatttaggt gtaaatgaag gtagctcaca agtagcaaat
    32761 aaccaataca tcctattgca tgaaacagca aacgaaactg cgacaggacg aaatgaagcg
    32821 cagtacatga aacgttcttg gtacaatgcc tatacagcgt atattgttgg cgatggtggg
    32881 atcgtttacc aagttggaca acctggttat gtacaatatg gcgctggttc atatgctaat
    32941 gcaaatagtc ctgtgcaaat cgaactgcaa catacgcacg ataaagtaac gtttgaaaag
    33001 aactacaagg catatgttga attagcgaga gattccgcta ataaatacgg tattccactt
    33061 acattagaca caccgtataa tcaaccagga atcaaatcac atttatgggt aactcaaaac
    33121 atttggggcg atcacactga tccgtatgga tacttatcag aaatgggtgt aagtaaggaa
    33181 aaactggctt acgatttagc tcatggattt acagacgaaa acccaacgac ttctgagaac
    33241 aagcctgtca ttgatccaac ccgagcaggt gcagcaaatc caactttatc agatggaacg
    33301 aaccattctc acattgatca gtttggagaa atcgaaaatg cgaacttaca cgtcgctgga
    33361 tggcacatcg ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa
    33421 gaattagctc gagtaagagc tgatggaatt tatagaccgg atgtaaatca agcttataat
    33481 acttctggaa acgttggcta tcatgtatct tttaacatgc gcaatttccc taataagaaa
    33541 gtatacgtca tgatgcgggc aacgaatgat ccagaaggaa acactaaagg cggtgcgcaa
    33601 gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt
    33661 gaggagcagt acatagaatt gaaaattaac gttaaatcaa aaaaatattt acaagatgtt
    33721 tatttaagta gataataaaa gaaaaaagca ggtgaaattg tgacagcaga aattggtatt
    33781 atgaataaaa gtggaatcgt gttagcttca gattcagcat ctacgattgg agatagcaaa
    33841 gtatataata ctgcaaaaaa attatttact ttggattcca tgcattctgt aggtatcatg
    33901 atatatggta atgctgaatt taatggtatt ccttgggaaa taatcattac tcaatataag
    33961 aaaagcattg gtagttctgt ttttaatact ttggaagaat acgcggataa ttttatagaa
    34021 tttgtcaaaa cagcatcttt tattagaagt gaacagaccg agcaagaaca aatgattggg
    34081 gtttttcaaa aaataatttc aggtttattt gagagtatag aagtagatat taacttttta
    34141 ataagccaag gtacacaaat agacaaagat gtcctagtga agttattaca aacaaaaatg
    34201 aataccaatt tgtctcaaca gtcccagaca ttcattttag atattgaaaa ggcgttattt
    34261 cttagtaact atggagaaat attaaaagat attcttaata gtatttctac aatggaaggt
    34321 gtttcagaag ctatatcaga ggaaattcaa agttacgttt atgaaattat aattagagac
    34381 gatgtttatt cttcccctac aggaatagtt atagcaggat atggaagaat ggatattttc
    34441 cctaaactat attcttataa tatgtttgg
//
LOCUS       DASJUF010000019        34448 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJUF010000019 DASJUF010000000
VERSION     DASJUF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.019"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            352..1041
                     /locus_tag="U4B62_000824"
     CDS             352..1041
                     /locus_tag="U4B62_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2141004.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="U4B62_000825"
     CDS             1028..2236
                     /locus_tag="U4B62_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2141005.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="U4B62_000826"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="U4B62_000826"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN2141006.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="U4B62_000827"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="U4B62_000827"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN2141007.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="U4B62_000828"
     CDS             complement(6339..7034)
                     /locus_tag="U4B62_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN2141008.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="U4B62_000829"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="U4B62_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN2141009.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="U4B62_000830"
     CDS             complement(8233..8424)
                     /locus_tag="U4B62_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN2141010.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="U4B62_000831"
     CDS             complement(8445..9335)
                     /locus_tag="U4B62_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN2141011.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="U4B62_000832"
     CDS             complement(9322..10089)
                     /locus_tag="U4B62_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2141012.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="U4B62_000833"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="U4B62_000833"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN2141013.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="U4B62_000834"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="U4B62_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN2141014.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="U4B62_000835"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="U4B62_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN2141015.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="U4B62_000836"
     CDS             14660..15043
                     /locus_tag="U4B62_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141016.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="U4B62_000837"
     CDS             complement(15211..15969)
                     /locus_tag="U4B62_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN2141017.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="U4B62_000838"
     CDS             complement(15987..16805)
                     /locus_tag="U4B62_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2141018.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="U4B62_000839"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="U4B62_000839"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN2141019.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="U4B62_000840"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="U4B62_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN2141020.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="U4B62_000841"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="U4B62_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN2141021.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="U4B62_000842"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="U4B62_000842"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN2141022.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="U4B62_000843"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="U4B62_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN2141023.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="U4B62_000844"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="U4B62_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN2141024.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="U4B62_000845"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="U4B62_000845"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN2141025.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="U4B62_000846"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="U4B62_000846"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN2141026.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /locus_tag="U4B62_000847"
     CDS             26847..27146
                     /locus_tag="U4B62_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN2141027.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="U4B62_000848"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="U4B62_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2141028.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /locus_tag="U4B62_000849"
     CDS             27734..27970
                     /locus_tag="U4B62_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN2141029.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="U4B62_000850"
     CDS             28113..30086
                     /locus_tag="U4B62_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN2141030.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="U4B62_000851"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="U4B62_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN2141031.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="U4B62_000852"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="U4B62_000852"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN2141032.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="U4B62_000853"
     CDS             32965..34257
                     /locus_tag="U4B62_000853"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN2141033.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10510 a         6702 c         6821 g        10415 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34441 tttccgaa
//
LOCUS       DASJUF010000020        32497 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJUF010000020 DASJUF010000000
VERSION     DASJUF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.020"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..563)
                     /locus_tag="U4B62_000854"
     CDS             complement(<1..563)
                     /locus_tag="U4B62_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEN2141034.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="U4B62_000855"
     CDS             complement(750..1400)
                     /locus_tag="U4B62_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141035.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U4B62_000856"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="U4B62_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN2141036.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="U4B62_000857"
     CDS             complement(2912..3910)
                     /locus_tag="U4B62_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN2141037.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U4B62_000858"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="U4B62_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN2141038.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="U4B62_000859"
     CDS             complement(4926..5657)
                     /locus_tag="U4B62_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN2141039.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="U4B62_000860"
     CDS             complement(5661..6926)
                     /locus_tag="U4B62_000860"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN2141040.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U4B62_000861"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="U4B62_000861"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN2141041.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U4B62_000862"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="U4B62_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN2141042.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="U4B62_000863"
     CDS             12268..13770
                     /locus_tag="U4B62_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN2141043.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="U4B62_000864"
     CDS             13772..14509
                     /locus_tag="U4B62_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2141044.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="U4B62_000865"
     CDS             complement(14680..15873)
                     /locus_tag="U4B62_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2141045.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="U4B62_000866"
     CDS             complement(15952..16158)
                     /locus_tag="U4B62_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2141046.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="U4B62_000867"
     CDS             complement(16151..17293)
                     /locus_tag="U4B62_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2141047.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="U4B62_000868"
     CDS             17805..18440
                     /locus_tag="U4B62_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2141048.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="U4B62_000869"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="U4B62_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18916..19032
                     /locus_tag="U4B62_000870"
     CDS             18916..19032
                     /locus_tag="U4B62_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN2141049.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="U4B62_000871"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="U4B62_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN2141050.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="U4B62_000872"
     CDS             21086..21253
                     /locus_tag="U4B62_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN2141051.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="U4B62_000873"
     CDS             complement(21313..21666)
                     /locus_tag="U4B62_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141052.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="U4B62_000874"
     CDS             22170..22589
                     /locus_tag="U4B62_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2141053.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="U4B62_000875"
     CDS             22615..22821
                     /locus_tag="U4B62_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141054.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="U4B62_000876"
     CDS             complement(23049..23222)
                     /locus_tag="U4B62_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141055.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23198..23863
                     /locus_tag="U4B62_000877"
     CDS             23198..23863
                     /locus_tag="U4B62_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141056.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="U4B62_000878"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="U4B62_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN2141057.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="U4B62_000879"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="U4B62_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN2141058.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="U4B62_000880"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="U4B62_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN2141059.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="U4B62_000881"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="U4B62_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN2141060.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /locus_tag="U4B62_000882"
                     /pseudo
     CDS             complement(29076..30241)
                     /locus_tag="U4B62_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(30257..30484)
                     /locus_tag="U4B62_000883"
     CDS             complement(30257..30484)
                     /locus_tag="U4B62_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN2141061.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31558
                     /locus_tag="U4B62_000884"
     CDS             30968..31558
                     /locus_tag="U4B62_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN2141062.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            31748..32461
                     /locus_tag="U4B62_000885"
     CDS             31748..32461
                     /locus_tag="U4B62_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141063.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DASJUF010000021        32228 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJUF010000021 DASJUF010000000
VERSION     DASJUF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.021"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..157
                     /locus_tag="U4B62_000886"
     CDS             <1..157
                     /locus_tag="U4B62_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEN2141064.1"
                     /translation="DLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAV
                     TGLNIET"
     gene            269..1720
                     /locus_tag="U4B62_000887"
     CDS             269..1720
                     /locus_tag="U4B62_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN2141065.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2069..2701
                     /locus_tag="U4B62_000888"
     CDS             2069..2701
                     /locus_tag="U4B62_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN2141066.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3289..4728
                     /locus_tag="U4B62_000889"
     CDS             3289..4728
                     /locus_tag="U4B62_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2141067.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5023..5241
                     /locus_tag="U4B62_000890"
                     /pseudo
     CDS             5023..5241
                     /locus_tag="U4B62_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5490..5705
                     /locus_tag="U4B62_000891"
     CDS             5490..5705
                     /locus_tag="U4B62_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141068.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5922..6178)
                     /locus_tag="U4B62_000892"
                     /pseudo
     CDS             complement(5922..6178)
                     /locus_tag="U4B62_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6365..7276)
                     /locus_tag="U4B62_000893"
     CDS             complement(6365..7276)
                     /locus_tag="U4B62_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2141069.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7295..8299)
                     /locus_tag="U4B62_000894"
     CDS             complement(7295..8299)
                     /locus_tag="U4B62_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN2141070.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8296..10419)
                     /locus_tag="U4B62_000895"
     CDS             complement(8296..10419)
                     /locus_tag="U4B62_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2141071.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10424..12871)
                     /locus_tag="U4B62_000896"
     CDS             complement(10424..12871)
                     /locus_tag="U4B62_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2141072.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12858..13247)
                     /locus_tag="U4B62_000897"
     CDS             complement(12858..13247)
                     /locus_tag="U4B62_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2141073.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13307..13810)
                     /locus_tag="U4B62_000898"
     CDS             complement(13307..13810)
                     /locus_tag="U4B62_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN2141074.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13823..14047)
                     /locus_tag="U4B62_000899"
     CDS             complement(13823..14047)
                     /locus_tag="U4B62_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141075.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14151..16061)
                     /gene="ltrA"
                     /locus_tag="U4B62_000900"
     CDS             complement(14151..16061)
                     /gene="ltrA"
                     /locus_tag="U4B62_000900"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2141076.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16807..16944)
                     /locus_tag="U4B62_000901"
     CDS             complement(16807..16944)
                     /locus_tag="U4B62_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN2141077.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16941..18125)
                     /locus_tag="U4B62_000902"
     CDS             complement(16941..18125)
                     /locus_tag="U4B62_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2141078.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18384..18674)
                     /locus_tag="U4B62_000903"
     CDS             complement(18384..18674)
                     /locus_tag="U4B62_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141079.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18664..19806)
                     /gene="dcm"
                     /locus_tag="U4B62_000904"
                     /pseudo
     CDS             complement(18664..19806)
                     /gene="dcm"
                     /locus_tag="U4B62_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19882..20046)
                     /locus_tag="U4B62_000905"
     CDS             complement(19882..20046)
                     /locus_tag="U4B62_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141080.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(20039..20713)
                     /locus_tag="U4B62_000906"
     CDS             complement(20039..20713)
                     /locus_tag="U4B62_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2141081.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20791..22131)
                     /locus_tag="U4B62_000907"
     CDS             complement(20791..22131)
                     /locus_tag="U4B62_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN2141082.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22214..22549)
                     /locus_tag="U4B62_000908"
     CDS             complement(22214..22549)
                     /locus_tag="U4B62_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN2141083.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22559..22933)
                     /locus_tag="U4B62_000909"
     CDS             complement(22559..22933)
                     /locus_tag="U4B62_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2141084.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22946..23260)
                     /locus_tag="U4B62_000910"
     CDS             complement(22946..23260)
                     /locus_tag="U4B62_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2141085.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23373..26600)
                     /gene="fss3"
                     /locus_tag="U4B62_000911"
     CDS             complement(23373..26600)
                     /gene="fss3"
                     /locus_tag="U4B62_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN2141086.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27388..27711
                     /locus_tag="U4B62_000912"
     CDS             27388..27711
                     /locus_tag="U4B62_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141087.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            27875..28792
                     /locus_tag="U4B62_000913"
     CDS             27875..28792
                     /locus_tag="U4B62_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2141088.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28785..29567
                     /locus_tag="U4B62_000914"
     CDS             28785..29567
                     /locus_tag="U4B62_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141089.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            29568..30248
                     /locus_tag="U4B62_000915"
     CDS             29568..30248
                     /locus_tag="U4B62_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2141090.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            30241..31137
                     /locus_tag="U4B62_000916"
     CDS             30241..31137
                     /locus_tag="U4B62_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2141091.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            31349..>32228
                     /locus_tag="U4B62_000917"
     CDS             31349..>32228
                     /locus_tag="U4B62_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141092.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAM"
BASE COUNT         9590 a         6473 c         5786 g        10379 t
ORIGIN      
        1 agatttatct ggactattat caatgttttc tcttgctata gaagaacaaa ttacattaga
       61 caaattgaaa atgttagatt atttatttca tcctagtttt agccaaccct ataattttat
      121 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
      181 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      241 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      301 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      361 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      421 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      481 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      541 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag
      601 tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat
      661 aacttcttcg aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa
      721 aacggaagga caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt
      781 ccaatcacaa ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt
      841 agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt
      901 atatgcttct gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt
      961 agctcaggca ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt
     1021 cattatggta acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt
     1081 tagagtgtat gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg
     1141 aattagtgga aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg
     1201 agaagcaact aaattcctta caggacggta tgcaacagat acacaatact atttgaagct
     1261 aaatgaatta attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca
     1321 aatgagtaag actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg
     1381 aaatcgtgga aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat
     1441 tcatgcagtt aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta
     1501 cgtggtgata caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct
     1561 agtaaaaact ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg
     1621 gaatagtact ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag
     1681 catgttagtt gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca
     1741 aagtaacaag aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg
     1801 atatctcgat tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta
     1861 ttattgccgt ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct
     1921 atcttgagga tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca
     1981 gactaagaaa aattggaaat atttttttct gatttattaa aagttaattg aaattatatg
     2041 gtacttcttt cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt
     2101 tgaggagatt actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga
     2161 aacgattgta tatcgatcaa acttactatc aggtagaacc aaaagttgtg gatgtggaaa
     2221 ttataaaaat aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc
     2281 aatcaagcca actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg
     2341 tgggagaaaa aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg
     2401 atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa
     2461 gtatcctttg gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg
     2521 cagtatgtgt gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg
     2581 tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc
     2641 aaaattaccg aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata
     2701 gattgcgtat ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa
     2761 gagtgcagct caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa
     2821 atatcgaggt aatcattaat tattgtggat aataattaac agtgatatat aaacatctat
     2881 aatatattat tcatactatt gatatttagt ataatcctat ggaattgaat atcatagcaa
     2941 tgtatttgaa aagcaaattg gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg
     3001 ggaaaaagta gaatggaaat ttgcttacgt ctcacgagat agctcacact attaatctga
     3061 agaacaatga agtcgacttt tagaaattgc tagaagaaag taaagcgaat tatcactagt
     3121 tactatagag taagttcaaa taaagcatct ccaacttaca tggttacatg aggatatcaa
     3181 atcgaaatta ctcaagcttc ctatggctag agtcgcgaga agttgttggg cagtagacat
     3241 aggttatatt gttcttagct acacgatcat tagaaaggaa atcctattat gtatagttat
     3301 caagaagatg caaaagtcca aaaaaatcgc tggtggattt tgattgctat atctatgttt
     3361 accttcatgt ccacattgga tggcagtatt gtcaatattg cactacctac catctcagat
     3421 gatatgggca taccaatgaa tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc
     3481 tgttcttgtt tgttgctgtt tggtaaaatt ggcgatagcc gtgggaaaat caaagtattt
     3541 cggattggta cgctgatctt tgtcttggga tcgctgttgt gtgggttcaa tcattcgcta
     3601 ggtattttat tgttcgcacg ggtggtacaa gccattggtg ctagtatgac tatggctaca
     3661 aatgccggaa tcatcacgga agtctttccc atacatgaac gtggtcgggc attgggagca
     3721 ataggtgcgt ttgtttcatt aggctcgatt gctggaccag gtcttggcgg cttgattttg
     3781 tcccaatttt cttgggcgta cattttttgg atcaatgtac cggtgggaat cgtaacgatg
     3841 ttgattgctg aaaaattctt accaaaagat atcaccatga gcggccatac aatcgatgga
     3901 gggggctttg ctgccattgc ggctacgatt atcagctttt ttggagcaat ctttttgggg
     3961 caagaaagtg gctttttcca tccagtcccc cttgtactgt tcgtaatttc agtgattgcc
     4021 tttgtcagct ttttaaaagt tgaaaagaaa gcagcctctc cattgatttc tttcggtatt
     4081 tttaagaaca agatttttac catgagcttg attacagcct tgttgatttt ttcttccaac
     4141 ttctttgtca atgtagtgat tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc
     4201 tatgctgggc tgttgatgat ggtctttcct ttgatgatgg tcgtcggggc gcctttaagc
     4261 ggctacctga ccgaccgggt tggcccggag attttggtat tagtaggctt gggcttattg
     4321 tcggtaaccc aggtcatgta tatgtttttg tcagtggact ctcctttgtg gctatacatt
     4381 gtcgctactg ccatcatggg tgccggcaat gccttgttcc agtcccccaa taatacgatg
     4441 gttatgagct cggtcactat cgaaaacttg ggactggcag gctcgatgaa ttcctttgcc
     4501 cgaaatttgg ggatggtcag cgggatttcg ctttctacga cggtcttata tcaagcgatg
     4561 tctgctaaga tgggccagca agtaactaac tatatcgcaa atcggccgga tgtcttttta
     4621 tatggaatga agattacctt tttaggcagc tttgttttgt gttttgttgc ctttttcctg
     4681 actatctggc gtatcaaaaa acaaggaggt attcagaaaa atgtctgaaa aaaccactgt
     4741 tgaagaattt ttggaggcct gcgtatgggt gagtgggatt tccatggatg atggaagatg
     4801 aagtgaactt ctaaaagccg aaaaactcaa gtattatctt caaaatacta gtgctcaatg
     4861 agatgcttga tttgatcaca agaagactgc gaaagccgct ggcaaccgca agctaggtat
     4921 cccaccgatg gactacgttt tagaggctac tggttatgaa tacggagcca acgctctcat
     4981 ctgcataaga atacatcacc cagattatgt aaagattttc gatacttctg tcaaaaaatc
     5041 attaagatag ataaatgatt tccgtctagt caaattggtt cagaatgtag acacacagat
     5101 ggtaagaagt cactcacaat tcgagaatgg acttgtccta tttgccgtat acatcataat
     5161 cgaaacgtca acgatagtat caatattttg accaagggct atgaatacaa gcattgcctt
     5221 agaaaacatc aagaacctta ggaacaacgg ggatagcttg gttaataaga gataccgctg
     5281 tttaggcttg caagtatact cttttatcaa gaattccgtg gctttagtca tgagaggctt
     5341 aatactatga aaattttctt tagcattttt gatttaggtt ccttttctaa ttataataag
     5401 atgaccacta cagtaaccta ctgaaatata taaacagcca ctgtagtacc attttcaaag
     5461 tagtctatga gaggagaatc ttcattctta tgaacgctgg aattgcggat atgaatttaa
     5521 taaaaaaaac ccttaatgat tttacttcca attcaatatc aaaaggtact ggtatcaatt
     5581 taagcacaat aaaaaagctg aaaagtggtg aaagatcagt ggaaaagctg aatcttttag
     5641 atgctataaa aataacggaa tttgctatga aaaatggaaa agctgagatt gaaatatgga
     5701 gataattatc gtctagtgaa ttacttgcca cttagctgaa tttaacgatc cttggtgctt
     5761 gaagggggag attcttggga atagagcata cttgcaagcc tatgtcttta ctaacaacga
     5821 tatctcttat taaccaagct atccccgtag ttctaaggct tttactttta tctaagcaat
     5881 aatcgtattc aaaggaaatt tttgaccctt agaaagtctc attttatcag acgtgagctt
     5941 aggcattgct gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt
     6001 attctttatc acgatttcat tcatctaagg taatgtgatg tgattctgaa attctttcaa
     6061 aggcagtttt tgcataagct gacattacat catcaatgac atgtttacaa tgtttcacta
     6121 ctaaaataag ggggtaacca agaagaaaaa ctgaatgatt atttgtatct aattccatgg
     6181 tatatcaacc atttcctata tagactgatt atgacatgga ttaaaataaa aaggcactat
     6241 gtcttaattg tcggttttcg agttaccgaa tggcattcat cacccaccta tagaggatgg
     6301 gcgacttctg cctgaaataa gttacactac ttataacttc taataaaatt agattgagtc
     6361 attattattt aacatcaacg attttccagt ttccattttt ttgtaatact aatttgtact
     6421 gaaagtagct agtggttttt gatacattat caaggtattt tatctttgta ctcactagaa
     6481 aattttttcc gttttgtaga taaactggat tctctaactc cgaaaacttt aagtcaatat
     6541 tgattggtcg catcgcacca ttttcaacat aatactccaa ttctttttca gaagctgatg
     6601 gataaagttt aaaaaatgtt tctaaaaaat cggtgatttc ttccgttgtt ttggcatcaa
     6661 tttctgaatc gttttctact tgttttgctt catattttgc cttgtctggc tttccaatca
     6721 ttgttggcaa actagttact aaatgattcc tgttttcatc ttcaaagatt gtcactcgat
     6781 atgcagaaga aattgattgc gtcttttttc cctctgtaat atgttgctcc actaaaaatg
     6841 aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttacagat tgaacttcgg
     6901 aactcgtggg aatatctgct cgaaccatat cttggattaa agcgagaccg ttttctgtaa
     6961 ggtactgttc gagattactc attctctttt caattgcttc tttgttattt ttccatgaaa
     7021 aatattcttt cgcaaaatcc tctgtaaaac tttcaacgcc agaagtattc actaccttct
     7081 cttttataac cactttttca tgagttgtat gttgatcaat ggcggtgaaa tttttataaa
     7141 cgccaaaagc aagactacaa gacaataaaa gccaaagggc aagcaccatt tttcgatgct
     7201 tacccacgga tacaactcgt tcttttttct tttttgggct cttttccttt tgattccgtt
     7261 caatttttat cttcattctt tttcctcttt tctcttattt gtatcggcca gctccgataa
     7321 gatgctgccg ccaatagttt tccgttagat tcgtccaccc cactggatct cctgcatgaa
     7381 acatgcgatt ctcccctgcg taaatgccca catgggtcac ataagtaccg gcatcatagg
     7441 ttgaatgaaa gaaaactaaa tcacctggtt tggcatcttt tagatcaata tgagtcatgg
     7501 catcatattg atcctgagcc acccgaggaa gtttcaggtc aactttggca taacaccatt
     7561 gggtcaagcc actacaatca aaccccgttt ctggacttga gccaccaaac acataggccg
     7621 ttccttcata cttaaatgcc tcatcaaaaa tttcttggac tattttgtca tcaaactgag
     7681 ttgtagttag gtattgcttc actaataaac agtaaaacat attgccataa ttataccgcc
     7741 acccaccatt ttccttaatc gcgattgggt tggggtaatc gactttttga ccacctgatt
     7801 tttcctttga aaaagattca gctaaagcaa aactatgctt cttcccgttt ttcccgacat
     7861 gatctagata cccgccacca tagttatagg cctgaataat actatcttga tcacacccta
     7921 gttctttgga acgcttaacc aaagacgcaa aatagagaca gccttgtttg atggatgcct
     7981 cggtacttaa tgaattaggt ggtaatccgg ctgactcaga actttgcata acgtcttcca
     8041 ttgtgccacc agattccact tgaataatcg ccaaaagaat tggaatatac tctgttatat
     8101 ccgattcttt ggcatacttt tccaccattg cacggtgttt caatacttct tctgagacac
     8161 tagttccgcc aggagaagtg ccagaactac catttccctc actgtcttca ccaaacaaca
     8221 aacccacaca catcaaaata cccataaaag agatggtcag aaaagttaat gaaataacac
     8281 ccactttctt caagttcatt ttttctcacc tgaccttgat tttggacgat ggttcattct
     8341 tttttgtact ttttgattgg tctgctttgg cagagtggac ctctgtttca cttgttggaa
     8401 agaatttttc ttacttgctg attggctttt cccttttgaa atgatttctt gactagttag
     8461 ttttcgtttg atttcgggat tcagatttgc tctcacaatc cgctttggtg cttctttttt
     8521 cagcatcata tctttctgag gttgagctgc tttatctatt ggaatattcg cccttctttt
     8581 tccctgacga tttgttgctt cactcagtat cttgcgtttt tcgtccattt tcgcatttct
     8641 tcgttgtcgt tgtttttctc gcaaatccac tcgatccgct ttttcttgga acagtccgcg
     8701 cttgaattct tctggtgctt ttttcgactt ttcgattcct ttatggagat tgtatttcaa
     8761 attcgttggc gtattcctta cctgatcctt tacttgttta gtcttatctt tcaccaaagc
     8821 ttgggtatcc aaaactttgc cgatttttct cccagccaaa ttcattcggg attgtttctt
     8881 attatttgaa actggattct ccttagttaa gtcagatagt atttcgtgat cgttcggtaa
     8941 tcgtgaattc tttcgtagag aagaaccgga tggcttaaat ttggattttg attgttcctt
     9001 tgccaactta tttccgactg ctgcaccacc accaaaagcc aaagctcgtc ccacatttcg
     9061 attgagttgc cgcaattttc gattcattaa catttgcggt tttcgcatga cgcgtcttcc
     9121 cattgactgg ctatcattac tctggaggtt gaacatactc ataatgtcgc ccaatttgaa
     9181 atagataccg gcaaaggtca caatttgcag aaaagcaacc atgaaaaatg gataggttgc
     9241 cgaaatattg aaaaacatcg tggagatgct aaatgcagtc gtaatcacca aagtaacacc
     9301 cgctcgcatc ataatcgtat tgaataaacg aataatcgcc ttctttccta aactttcata
     9361 agtcggcaac atggacaata agaaactaat tggcagaaac atcgcaaaga taatgaagag
     9421 aatttgcgag aacaacataa tccctgttag aagaaaaaca aagaaggaaa tgccaatatt
     9481 gaacaatacc aaaaagacca cggtccctaa acggttcatg gtcttggtaa tactgagatt
     9541 cgcattgtca ttgtcttcaa tctctgcctt gaccgcttct tcccgatctt tgcctttgtt
     9601 ttcatctgga ctcacagata gaatcttgtt gacccggtct tcaccaattt cttctatgtt
     9661 ggaatcatca aattgcagta gtaaccaagg ttgttggact tgaatcgaaa acaaactatc
     9721 ccgaatcaaa tccacactat cttttccttg gctttctgaa ttgggtacaa cgatctcggt
     9781 tccaagggta agggccgctt cactaacatc tgagctaaag tcattgattt ttgtgatata
     9841 ggtaggcgca taagcaatga acgaacccga tagtagaaaa atcatcacaa aattaatcac
     9901 ggctcgaact gctttcgtgg tttctcgttt taataatccg gtatacgcca catacacccc
     9961 cagagctaaa atcatcaata atagaaaccc aaaataaaat ccggaggtct ggagtccatt
    10021 ttcagtaata ccagccaatg tttggatatt ctttccaata ctttcagccg tatccgaaat
    10081 gaaatctaat ttgtaagcct cttgaaccac ataacccgtg gcgttcgaca aatacaaact
    10141 caccgtccaa atgaaattag taatggcata aagaccatac atcacgcttt tccctaagcc
    10201 atcaccccaa ttccaaggca accaatccca agagctatcc acaaagaaat ctagttgata
    10261 gttgttcagt gaatatttgg aatatagatt tcctgcttca actgtatcat caactagccc
    10321 tgtcgcctcg gctaccgtac caactaaact caatagaact atcagagtga gtatgctaat
    10381 aaaaacgatt cccacaatcc gaaaaagttt ctttttcatc ccctcactcc attctctctt
    10441 gaacaggtgg acgggtatca aaggcatgga agagttcttc aaagactgga tgtacctgaa
    10501 ccacgcctac ccgaccatac aaatcctgca tcaagcattg gccattttcc aaatctcgta
    10561 ggcgtttttg attgctttca tcttctcgat ccaagccaaa gaattccaag gttttcttga
    10621 tttcatgaat atcggtactg cgaaacgcaa acttcaaccc gatattattc ttcaagcgtt
    10681 catccagtaa atcatcacta ttttgcgtga caaagtaaac cccggcattc atagcccgcc
    10741 cagcacgaac gagcttattc gataatgttt tcccttgcgc tacttgaaga aaactccacg
    10801 cctcatctaa gtctacaatt ttgaagattt ctcgattact atggataaag tccaaggcaa
    10861 aagtggaaat gacgattagc atggaaacac taagcaattc catggtggta tattcttcaa
    10921 aagtggtttc tgcatctggt aaaaccaaat ccgctacttg aatgatattt aactgcctat
    10981 ctaaactaat cgactgggta atcgaaccat ctgagaaaag caaatgggca aaatcataat
    11041 cggtaaagga ctcgatgtga tccgcgatat tttccgcaac tggtgagcct tcttttcgaa
    11101 gctcattaat tacttgtaat aatccccgtt gattgctttg ggtaaccgtc cgaatggcct
    11161 tacgcaacac tggaaacttc tccccatctc gactagaaat tcctgttaaa aaggtcagaa
    11221 tatcaattgc tagactttca gaatccttct ttttctccat aatcacataa ggatcaagta
    11281 atccttgatt ttttggctta ctagttaagt tgagaatatt gatttcatcc gcaatttctg
    11341 gcaaggtttc tttccagcca ccccgttccc cttttggatc gacaatcaca gcttgtccac
    11401 caaaaaggac ggcgtaataa actaagagat tgttactaaa ggactttccg ccaccgagtg
    11461 aaccgaggaa tgccgctgct agcgcattgg taactgaatc cttgacccct tgtgaggcca
    11521 atgccggttt caggtaaaca ttgcgccccg tatctacgtt ataaccaaag taaatgcctt
    11581 ccaactcccc taacatctga gtggcgccaa aaccaagtcc ggcgagaaaa tcagacgtaa
    11641 catactggat ataatcattc atgtatcgct tactagaagg taagaattct tcatgtagcc
    11701 ccatcatatc gccaaaaggc cggactagtt ttacattggt atcatcataa aaatctagca
    11761 cttcatcaca ccggcgcttt agttcatcta ctgattcggc acttactcga accacatagc
    11821 tcagtttgta catggattct ttggactgat ccaaggtagt ttccaattca tctacggaat
    11881 ccaatgcatc taagacattg gaatttgttt cattgtcaga ttgataggca tggttgtcca
    11941 agtcttttaa ttcctttttc ttattgcgaa cggtagcaag tgcctttttg ttcgtcacaa
    12001 tctccacatt catactagtg tcaattggga atgtaaattg ttgttgctgg taatagaaaa
    12061 gttctgagga gggaaactcc atttccccca caattgtatt aattgttaag tacgctacat
    12121 atgattcatg attctcatgt tccatgcata agtaccgggg cttttcttca atcaaacaac
    12181 gactgggacg taataagtcg taacgtttca ccaaagtttc cgacttcagc tttttctttg
    12241 gtagctgaaa ttcatattct tcaaatggtg tacccttttc gccatagata tgttcaatca
    12301 aatacatcag atcactcggt gtgactcgtc tgactttaaa tcttcgagcc aatttacttt
    12361 ccattagttt ttcaagcttg gtatatcggc gaatttcttc attggataag gaaacaaaat
    12421 cgcccatcaa atgatgattc actccataga caaattcttg aaagccggag aagaaagatt
    12481 tcttgagatt ctttagattg acctcttcat ctgtcgcaat cagtttgaag ccaataaaaa
    12541 agcgataatc aatctgacta tcgccaatca tggatactaa tgcctctgtc tgtaaatcaa
    12601 ttcgttgttt cgctacttct gttaatcgcc cggtaatttc cttttttgat cgttcttgcg
    12661 tggcacgaat actactttct gtggcaattt gtaaggcatg aatcttgcct tcacgatttt
    12721 gcgcaatcaa ttgacgaaag ttatcgtgga cttgatattt ctgttcaggt gacaaaaagg
    12781 agtaattgta aggtattaac tcatagtagg caaaacattc tccctcttga ttaaagacaa
    12841 gattattttc aatatactta atcgggtaca tagttcacac tccttaccag agtaatgggt
    12901 tcgtcccatc gtttcttctg ttctttgact ttctttcctg cgaaagtgac cttaaatcgt
    12961 aagtgatagc taataaaaga tcgtaaaaat cccaatggtt tcttaccgtc aaacgttttt
    13021 tgcgacatga accaagtaac tgccaccggt atacctaagt attttaaaaa ggccccctct
    13081 atcatagata gtggaggcac atgggcaaat acgatgacaa acagcaagga taaaacaaac
    13141 caagccatct ggttaaaagt cacaggaaaa ggcagttgaa agtcattaat tgcataaatg
    13201 accttttcaa ctgcccaaat actggtgtag ctttttattt tcttcatcaa atcattcctt
    13261 taaaaagaag gatagtttag aaatcaacta cccttcaata attttgttaa tatggatact
    13321 caaaaacacc tcgactcgtt accaaataat tgcctccaat ctccaagtct ctaccacacg
    13381 catcaaaatc aatatacgtc ttcaagtgtt ctggtacttc tccaaaatca ccagcttcta
    13441 caatcaagaa atatgctaca tcactcatat catcgcaatc tggataatag ataatatcat
    13501 ccacatgctc taccatctcc tcaaaagaat tgaagaaagc atgttgaatc tcagaagcaa
    13561 cttcaccaac tggcgttcct tcaagttcct ccgctaagcc acacaaccga ttaatttcct
    13621 caattggcgt atactcatca atctcaaagg gaagttcata atcatgaatt gcatattcct
    13681 cgtattcgtc atttaaccca attcgttctt tcacttcatc aaaatcgaca ggtggggtaa
    13741 accacgcccc caccaattca ccttcgttat atttcccgag gttcgcaata taaacacgca
    13801 tctgttccat cgtgcttccc ccttacgcac cgacaattcg gttaaacaaa ctgagtaaga
    13861 catccttcac cccagaagca ttaaagacca atccgaccgc aatcaaagca atcaccaaaa
    13921 agccaatcag tttggaaaac tcccgcttaa atcctaaaaa caaaccaatg accacaattg
    13981 ccagtaagac taaggattgg gcattggtta aaaaccattg atatatggat aggtaagttc
    14041 ctgatattat aaatatctca aacttttccc cccattcaca cggagcgtgc gactttcacc
    14101 gcactccgcg ttccatcgtg tttatattga aaagtattta tgtagtagac ctatatcaag
    14161 tttaattctt gattgccagc taatactcta tatttattta gtcttatgag ctggacttta
    14221 tttagcttca atagagctat atatttttgt attgtttctt ctgttttagc gtgtattaga
    14281 cggtgtaccg cctcttttac caaaataaga tttttataat cgtcatttcc accagctcca
    14341 gttggtacaa tatgatgaca atgtatttca ttagcttgtt gaaattcttc cagagttata
    14401 gcacattttc catgttgagc agaaaatagc gaaattcgat tgtctgcgta ttctattgtg
    14461 cgattttcca aagataattt cattaactgg tacatcagta atttatcaac acttagattg
    14521 gtgtggataa attttcttcc ctctagagtg tagcaattcg ctaaatacac ctttgccatt
    14581 ggttttctgt gttggatata tccaacaggg aaaataggtt cgccctcgcc agcaacatac
    14641 cgaagcattt tagatttacc atatcttgtt gcctcctttg tcgtaagttg tctgcctgtt
    14701 tttctcaatc tgcccctttt gtttgtacct aatctgttag tgaagatagc cattacagct
    14761 ctattcagtt tcatacaatc cagatttaca ttcgttgcaa tctgataata attttgcata
    14821 cctaatacca ttgaattata aagtcttgtt tcttctaatt ccgttttccc ttttgaggga
    14881 gaagcaattt tctttgcctg tgataccagc ttgtcttttt ggtttttgaa ttgtttatca
    14941 cttatatgtg atttaacaac taacttcttt cttctcggta tcattttaat cttgaaacca
    15001 agaaagtcgg agtatctttt tctaacattt acaattcttg ttttttcttg tgatacttcc
    15061 aattttaatc gttgttcaat ccattgagtg attgctattt tggctttttc agcactttcc
    15121 ttgtagcggc agaaaactct aaaatcatct gcataacgaa ccatatacat ttcctttaac
    15181 tttgttttct tcatttcttt gtaagcgttg cttttacatg ggctaccgct tttattgata
    15241 tgaaccacat agttttttgt aacaggattt tcctgccatt ggctttctac ccaatggtct
    15301 aactcattaa gaacaatgtt tgctagtagt ggggatatta ttcccccttg cggagttcct
    15361 ttctctggat atgttattgt accgttagtc attttgattg gagcttttaa tatccttttt
    15421 aaaatgaata tcaagtgctt gtcccgtatt cccatagccc atatctgttt tatcagcttg
    15481 gaatggttca cgttgtcaaa gaaaccctta atatcaaatt caatcacata atgtagctgg
    15541 ctgatttgta ggcggttata ggttgctttt attgcatttt caactgacct attaggtcta
    15601 aaaccatagc tgttttcact aaatttagct tcgcagattg gttctaatac ttgttttata
    15661 cattgttgta taagcctgtc ccacatacaa ggaataccta gaggtctggt tttaccattt
    15721 ggttttggta tttcttttcg cctcactggc ttaggtcgat acccatgtgg actgcctgca
    15781 acaatgtatc ttactttttt gacaactgtt tctgctggca atttgccaat atctttaatt
    15841 attgtaccgt ctgttcctgc tgttttactg cctgcatttg ctttgatatt tcgatatgcc
    15901 agtaatatat tttctctcga taggataata tccataagat gagtgaaaat atctccattc
    15961 ttacttttgg catataaatc atcaaaggtg ttttgcattt cgtaatattc agcgtgtctt
    16021 aaatcttcta cacacaatgt ttcattttta tttttaggca taaggcttca ctcctttact
    16081 tgaaattgag tgagcttttt tatcttactc gtaatcctta ttgaaacaac ataatacttt
    16141 tctaatatat acacgacttg agggcattcc tccattctca ttacaagaat ttcttaggtt
    16201 cgccctctgc ttttcagcat tagttcaact gcttattgtt cttcgtcagt ttattctctt
    16261 gatgagaacc caataccttt cctcgttccg ataatcttat ctatacatat atatccttag
    16321 gtcactagct ataagcctgc tggcttgata ccgcctgtaa cgttatatgg catttcataa
    16381 cctatattct tactttgcca caccagctac gatttctcgt acctttgcat ttctacaaag
    16441 ttcaaattta gacccgtaca ttcgctgttt cgtcagttcc ttatagaaca ttctaaccat
    16501 agatatttta tagacccccg acctgttgga tacgctaccc acctctggat agttttcgat
    16561 agaagatata tgttcatcaa ctattacggt atctttcagc cgtctttacc gagcttcaaa
    16621 cattctgagt ttcctcatac aatgcttgtc ggaggctcag ggcaggcgtt tcagagcgtt
    16681 acttcatcat tccacctgtc agattatcag ttctcctaat aaccaaactt tttcgttctt
    16741 ttattagtgc cttagctttt cactaaggaa cgagtcgcac ataaattttg tccgaaattc
    16801 atcttgtcac tttcctttct tttccattgc tcgatccgct gctgcacttg cattcatcaa
    16861 gcacatcaca gtaacaccaa gcatactgcc accaaatacc aaaaaaatat cttttacaat
    16921 tcccatgacg attcctcttt tcatataatc aaattttcaa ttgccatact ttgttgctcc
    16981 aaaatctttt tatgtcggtc tgataagcgt gcattctgta ccatatcctg gatataggtt
    17041 gtccgattca atcgatccaa ctccttagcc attttccacg taggggcgac ttgtcggcct
    17101 agccaattca aggttcgttc aatggtatac ggttttggct cggaagttaa ttgaatctct
    17161 ttccgattcc gtccaataaa acgcccccat tgttcattca agggccagtt ggttcgcctt
    17221 ttaccttcta ctttatccgc aaatcgcaag taacgattga taatggaaaa cgtggtgctc
    17281 tcaatgctac gaccttttaa caaatcttga atcgcatgaa aagcacggtc atttttcagt
    17341 cgaatctcaa aacgattttt cgtctcagtt tggtctaatg gaatccctaa cttgatatat
    17401 tgttcatagt ctttttcata caaacagaaa taaacctcac tcttgagact gccgatatat
    17461 aaggtattgc ccatgccgtc cttttcatca gcctttaaaa gttccccgga acgataactt
    17521 ttaaaggtac gaaacaagga gatacattct tccttctgac attttttcgt tagctcaggt
    17581 atatccaata gccccactcg atcattgatt gccaagtcca agcgtttcat cacgccaccg
    17641 gcctttagac aatcctcgaa aaagtcgtac cagctacgct tttgagcgag taaaaaagtt
    17701 tcaaattgtc ggcaacctcg cccctttaat tctaaaagca cacctttatc ttcttcatga
    17761 gacaacatca ccacaatatc acccatgaca tactgttctt gataggagta aaaggcataa
    17821 tcttcatgga gcatatactc aaatttcaaa tggagaattt cttgaataat ttttcgtgca
    17881 tccgttgtgg gaaagcgaat ccttacatag tcaaacagta acgtcaaagg caaatcggga
    17941 ttaaagcttt ccagttggtc aaaaattttc ctcatggttt ttatagatgc ggttacgcca
    18001 ccattttcta tctgatttaa gtacggccga ctaagtccac aagccgtagc caatttattt
    18061 tgtgaaacgc cgtattcgtt gcgtcggtct tttaatagcc gataatctaa attccgttgt
    18121 gccaagttct cacctccaaa ttcaaggtgt taactttttc aaatgagtta acacctatac
    18181 aaattactta ctatctttga tacaacaagc attcttaatg gttgcttact tattttttgt
    18241 gtttttgtac ccccctgtta gatacggggg tgagacatca tggtcggcta acgccgtcca
    18301 tgaacaggca cagcaccacg gaggatttct gcttcgcgaa tcctccgcgg tcagcgccta
    18361 acagtcagtt gtgttggttt aggttactca tctgtaatac catttacttc caactctgct
    18421 ttcatttcta acaagtattg acgaatatct ggttcttttg cttggtccgc aatccgctca
    18481 aattctgcaa ttacttgatt tatttcaatt cgagcatccg tctcctcatt cattaaatat
    18541 ggatcgccag cgactttgac accattcttg cgaatatcaa attttaaccg tcgcaattgt
    18601 tcttctaatt ctgggtctga ctgaatcttt ccaatcaccc aataaatttc tccaatcaac
    18661 tcatcataaa ccatgtacat ccaactcttt taatcttctg gcaatatcaa aaatgaccgg
    18721 aactgttaca gagtttccgg cttgcttata caactgacta tctgaattga cttgagaatc
    18781 tcgatcaaaa gcccaatcgg gaaagccctg aagtcgccaa cactctctag gtgttagctt
    18841 ccgaatttga tactgatccg tgacgatcgc ttgttggtct cctgttagta aggtatgaac
    18901 actctgtttt cccagacgac ctcggcgact tgtggaatta ggaaaagcca gattcacact
    18961 gtcttctggc ttcacctctt gatattctgt ttcatttttt cgaatcatga tttttgtacc
    19021 accttgtttc gttgacaagg tgggggcggt tccagtaact tcataaactc gattaccttg
    19081 ttcaaacttt cccggtaata gtcctgctac agagattcca tcccttgctg gcagtgcgat
    19141 tttgattcct tcatcacctg ttaaggttgg cgctacgcca tctgaaccat agacttgtcc
    19201 acccattcct cgattggaag gattgatatt taccaaattt ctgatgtttt gatcagatgt
    19261 tgaagacggt ccaatgatag gaaatacttg ttcggtacat gcgtctcgag aatgtccgat
    19321 aaggaatatc cgctcccggc tttggggaat gtaggccttt gagttaagca cctaccactc
    19381 cacgtcatac cccaatccat ccagcgttcg gaggatcgtc tcgaacgtag cccctccttc
    19441 gtgattgagc aatcctcgga cgttctcaag gaataaaagc ttaggtcgga gaagagtggt
    19501 gaaccgagcg atttcaaaga ataaagtacc tcgagtatcg gaaaatcttt gccgctttcc
    19561 cgcaattgaa aaagcttggc acggaaatcc gccgcaaagg atgtccactg gtccgaggga
    19621 ttgaataaac tcatctgata cacttgtgat gtcatgcatt tccacctcct tacttgtatc
    19681 atgaattgct ttataactac gtctagcaaa tttatcaatt tcacaaaaac cgatacaatg
    19741 atggctcgcc tgttccatac ctaaacgaaa gccaccaata ccggcgaata aatctaaaaa
    19801 tctcatacat tacctcattc attgcaaaga aaaaagccaa gaacattttg ctcttggctc
    19861 atacattatt aatttgtcct actatttttt atttaatcca actaatttcc gttgtttttc
    19921 attggcctga tcggctacat atttaatcac cgttttgaac tcttttgcag tagcattttc
    19981 cgataaatcc gcaatgctct tattgtattt ctttacaatt ttcgttactt gttctttctc
    20041 agtcatgtaa ttgaacctcc aaatctttcc tactataccc tagtggtaaa aataattcta
    20101 gattatccgt agcataatga acttcggaaa tattattcgt ataatctttt attatgatat
    20161 gaagcaatac atctggatgt ttactcttta cttttgcaat gttctctctt gatttgaaaa
    20221 aagcgttaat aaatgccttt ttcggcacat gacgtccctc tgcaatttca cgttcttgtg
    20281 taaatttcca agcgatttta gggtcttgat aaacatagaa aatcgtcact cgaaaatgtt
    20341 ttttcaacgc ccgcgagata tttttttcag cacttaatat tgcaaaagtt gcatccagaa
    20401 taaaagaata gccatgatct aagacatatc taaatgcctg ctctaccaac caagaagatg
    20461 ctttttgaaa gagactagag ttttttccat tataatcagg aaactgggtg cgaaagtaat
    20521 ccgcatcaat aatcacatgg ttatcataat tctcagctaa tccaatggca acctctgatt
    20581 ttccagcccc aggactccct gccataaaaa ctgcgtctcg tccaacttcg ggggatttcc
    20641 ctttgataat ataagaaata aacttatcct tatttgcttt tgcatactct aaataagaat
    20701 cattactttc catattaatt atcccctttc ttcctactat ataaaagtat tatacgttac
    20761 ttgaagtata aagtcagtat tgaagatcac tcaatcattc atcttttcta atttactaat
    20821 tgaatctaag aaatcatacc cttttggtac aagtggcgta taaaactcac taatcacact
    20881 gccacctgta tccacataac ctcgaccttt gatgtgcttc ataaagaagt ttttttcgac
    20941 ctccccaaac atcatggaat atccgagttc actcatacga cccaaagcca cgcgaaagtt
    21001 gaattgatcg cggattccgt ctcctaaata tttcgcatcc ggcctttggc aagccaaaat
    21061 cagaaagaaa ccagattgac gacccaacat cacgatttgt ttgagcttat tcaaaatcac
    21121 cgcactttct ttcgtcgtta acatctccat ataagccacg tattcatcaa agattaaaaa
    21181 gttgggtgga agtccaagat aggcataatt ctctcctgtt ttgtagttag acatttcttt
    21241 cattgccttg ctacgagcca tcatacgttc atagaaatct tccacacaag cagaaatttc
    21301 ttccttttgt gaataaacat gaggcatcac cgtacctaaa tcagctaaat ctgcattttt
    21361 gggatcgagg ataaacaatt ctgcatctga cttcaatagt gcttcgataa tggtgaggag
    21421 gaaataggtc ttccctccac ccgtgccacc agcgattaac atatggggta aagaatcata
    21481 agcccatact tgatttttca tcaatcgcag agtcccattt tctgccacta cttcgtctat
    21541 cccaattcga ttggcgatca tatcatacag taaagtatac tccacatagg agtctttgag
    21601 ttccttttct actaactcac aatacagtcc gctttctaac ttcttttcca attttaaaag
    21661 ctgatcttgg tatttcccaa gtgaaatttg aacgcgaata gacagtaatc catctttcat
    21721 tcgataacag attttgggga agtaactgat agtctctctg gttcggctac tattcaaatc
    21781 tttgaaaaag ccttcacttt gagtttgttt cacttcatac caatggttct ctagaatcat
    21841 ccgagctagc ttttgtcgat gccacaagct tcgccaatga tcaagaaaaa agtgaatata
    21901 aagcaatatc atagctcctg aaaaagcgag acttccaagc agacatacta aatcttttat
    21961 agaccaaacg atttcaatag tagaaagtga tagcttagaa atatcaaatt gtctaaactc
    22021 ttgaaaataa aagacgctca tccaaccgat gaacagagcg agtagccagc caagaacaaa
    22081 gtgataaacc aagtgctgat ctcctgcgcg tatgcgatgt cctttgtaca ttttgatggt
    22141 ttccacctcc aatcgaaaaa gcccaatagg atttcattag gcaatttcaa aaagttatgc
    22201 tagtcattta ctgctacgag gaggggagtt gtttcgtttc aaccaattga tttaaaaagc
    22261 cttcaatagc atccggcatc tccacaccaa tttcttccaa aatggattct gcctgccgac
    22321 ataagttttc atttacttcg acaactataa agaaagactc ttcttctcct aaccagtcta
    22381 gtacatcaga gtcttcttca ctgtcgctat tttctccaag taaaccttga tgtataatca
    22441 gaaacatttt ttcatcagag gttaatgctt taacgagttg ttcctcagtc agctcgcctt
    22501 ttccatattg atcgagacaa tctaaaatat gttcaacatt aatttccata atcttcacct
    22561 atttctttct ttcattttgg ttcgtaggat ttccttgatt cggctgccgc ttcaagacta
    22621 aatcatccgc cttaatgaac caattaacgg tagttccttc tcgaaaaaca taattggcaa
    22681 cggtatccac gattggattg accaactcta cctctgcatc ataagcaaac tcgcgtaagg
    22741 gtacattagc tgggatacta acttgaatca ttcgcccttg tgcagaggat tttaaattat
    22801 aggttcgttc ttttacctca ttaccaattg ttccatcctc attttgcttc cgtacttctc
    22861 ggcgtaatgc ggaaaaacgc aacttcccaa atgttaaacc tgaatctacg acaatacctt
    22921 ctgttaatct catcttcttt tcctcctatg ctttcactaa atcatcaaca tacaacacgt
    22981 aatcagtaaa cccacgattc tcaatctcat agcctttggc aaccaaatgg ggattcacgg
    23041 cttttagcgg ttggtcttga tcaaaatcct tttcaccggc ttccgcagct actaccacct
    23101 caatatcatc ggcccgttgc ttactggaat acaaatgata actgcgacca atcaccgtag
    23161 tattccgtcc atatccttct gttataattt ctccttcacc ggcataactt aactttccaa
    23221 aggtttcagc catatctggc acgacaaatt tcaattccat cttcttttcc tcttttcatt
    23281 gttagttttg gatacaaaaa tagcgcgact acttttttag gaggcggtcg cgcacgttgg
    23341 ttcttaggat ttacttgtat catctcatca ccttagttcc ggcgattcca gaaataatag
    23401 cccgccgtcg caaaaatcaa agtaaaacca atgaacagta aaacattgga atttttctcc
    23461 ccggtttgag ggaaggtctt cactgtggga ctagattctt gagagccact tggaacagtt
    23521 ggtgtttccg gttgtttcgg cgtacttagt atggttggaa cttcttttgg tgttaaggtt
    23581 tgagattttt ctttcaaatc ttcattgtgt tttccattga tattcccatc tttatcgtaa
    23641 ttgatttccg caaaagtaaa cttggttcct tctggatatt ttccggtatc tacttttttc
    23701 gcaagcacag ttttggtgaa ttctttatca tttacttcat aatcaatctt gccagatttc
    23761 caaatttctt tgtttgtacc atccggtaat aaagcataca gaattgtttc gaaagcttct
    23821 tttgagccat ccagtacatc atgagtaatc gacacatcat caaacatatc caccacatca
    23881 ccatgagtaa aggtctgaga accgttttct aggtgggcct ttgtttggat agaaacatgg
    23941 tgttccactg tacaactgac agtttgggct tggttattca agctaacatc cttcgctact
    24001 ggcttatcgc ctgcttcaaa agcttcctta ttttcataca catagttgaa taagacaata
    24061 gttttattta aaacttgttc ggcggttaat ttatgctgaa ttttccaagt tcctgttttc
    24121 tctttgttcg ccaacgttgc gcttgtttct gcaatcacca ctggtttggc tttttcatct
    24181 ttttcttggg cagcttttgt cgcatccaca tcaatggctt gcgctaccac ataccagtct
    24241 tctttgattt catgtagatt gtaactcaat ttatcaacta gctcggtaga atccagagaa
    24301 gttaattctt tgtttccgtc tttccaagtc gcaagagaag ttaaactatc ttcttcctca
    24361 ggcaaatcat agagcattaa acggttcaaa ctaacagaaa atgctttgtt cgtcagcttc
    24421 tcgtaaggaa cggtcaccgt tttaattggt tgtttttggt cttgttcggt aatggtaaag
    24481 acatactcac tcttcacaaa gtcttctttg ttttccttaa aagtagaacg gatttctaat
    24541 ggcgtaatct tttgaaaacc ttctggggct tctacttctt caagtaaata atccccataa
    24601 ggaagatttt caaacttgcc ataaccatca aaccctaatt gctcgttata agctgtggtc
    24661 gcttcatctt cggcacctgt gatttcattg gtcccttcca atggcgacac tttaaaggtc
    24721 agatcattga agccagtttc ggtagtgccc gcagctgatc cagcaaattt aaagaaatca
    24781 aagccaaagc gaatcacttg ttcttttgcc gtaacatttc gggtaatcac ggcatttttt
    24841 tcgctatcat ccaccttttt gatggataca ggatactttg tttcatcaag gctatatcct
    24901 tcgggcgctt ttgtttcttg ccaataatac tcgttgatag ctaaatgttt aacggcaact
    24961 tggttctttt cgtctaaagt caaagtgacc gtttcatcag aacctttcgt tcccttcgct
    25021 aactctggtt taaaagcctc actccattta acagcttttc catccttagc agtaaacaag
    25081 gtgtattcag caccctcaaa gactgcctta ccttgtgctt tatccccagt atctttgtct
    25141 tctttcgtca aagtggtttc gccagtgact tcttgatttt gcccttttac gttactggta
    25201 acaggggcta cggtttgatt ggcatacttc aattcgactt ttacaggttt gaacgtattc
    25261 acgaacccat gactagcttt agtttcggtc acataatagg ttcccaattc caacgcatta
    25321 gcaatctctt ttggtgtttc cgcatgaccg ttttcatccg tggtcatttc ttgaacgatt
    25381 tcaccggttg gactgtcttt gcgaatggca aacgtattac cagctaagga ataattgtca
    25441 ttccaaagat cactccctgt ttcaactccg cttttatcca gaatgatttg ccctttttcc
    25501 cgtgcgtttt ttgaagtcac gtaaatggtt tcgcctgctt taatggtggt agtcatgggg
    25561 gtggtatcaa tcgtataagg cgccggtact gatttttcgg taatggttac ttttgtacca
    25621 tgaggaatct catccaatgt agcaatgcct tctttgtcag tagtcacgtc ttttgcaggt
    25681 aaagtttttc caaagtctaa atggaaaacc gttcctggta caacatcacc cgattcttta
    25741 tcgacttttt tgatctttaa agaaccctcc gtttccacat caattttgat tgtgtaggta
    25801 ttaggtttat caatcgcccc ggccattaaa gtctgttggc cagccttttt ataggcgaca
    25861 ggtgttccgg taccagcaga tttcttaagc gtcagcacac cactttcttt tgaattggca
    25921 tttggggtga ttatcaactg attaccattc acacgataat caatgtttgc ggcattttcc
    25981 acaacctcat cgaactctga caaattcaga ttattcgtat ccgtcacagt agtcgattgc
    26041 cctaatactg ttttagccgt actattatgg aaggttggtt tcttctgata atcgtcaatg
    26101 gcttgattta tttttgcctc aatggctgcg atatttacgg cactaccatc tgatcgtttt
    26161 attgagtgaa gggtataccc attaacttct tgccagatca tcttctgtgc cacaatatga
    26221 gtatctgcat ctgtacctac ttttttccat aaaacggaaa ctaatttggc tttatcggac
    26281 atatctggta acgggttttt ctcatagcca ggagtgaact cattgtaaat cgggactcct
    26341 ggttcaatac agaaaatatc ttgtttaaag ccgttgtaca ctgcataatg cggaacagtg
    26401 acttctgaca ttggacgacc atcactaaaa gttccttcaa cagcatattg atgtgctaaa
    26461 tctagttcta cggttaccgt ttgtggatgg gtaatttcat cagcaaaagt aatcgtagga
    26521 gcaattgctc caacaattgc ttgaacagcc aaactcaaaa tagttaatac acataatttc
    26581 tttttttgtt ttttatacat gaaatgttct cctttcaaat taagcacaaa aaaataagat
    26641 gctcgcttga acatctttgt ttataaagtc tttttaaaat ttttattgac tgaatagaat
    26701 ggatttgaga atgaatgagc ctaaaaattt aatctaaaat acgaaatata ctgcctttaa
    26761 attagaacac attttagaac acaaacattt caaatttaat caaatataat aaaatatatt
    26821 tacgggaaaa cctttataaa tcaaggtttt tcaaacttat tcaatgatac gaagaaaaaa
    26881 ccaggtctta agaaagcccg taaagcttca cagttctcaa aacgttaatt gcgattatcg
    26941 ttcgagttca cgagttacga gaatcgtact gggcgaagca atgctgagcg cagttacctt
    27001 aatcctaact tttctggcac tttccacaat ttggaaagtg ccttttttgt gtttttagct
    27061 atgttaatag gtaggattct tggtctataa tatcattcga tctataaaca aacgggaaaa
    27121 agataaagta tcatcaaaca atattattta tccgcttctg aggttgtgac agaagtggtc
    27181 agcttcaaga aataagaagg aatttacaaa aattgcttct catgtttgat aaatattgtg
    27241 acttgtgacc gaggagttgg caggagaaca ccgtttattc ggaagaaggg attgtgaaaa
    27301 gtcttttgtc ataacctctt atttggtagc taattgtaaa tcccaaacgg catgtattat
    27361 ttttcataag tccgttttta aggattaatg ttttcttttg ctttttatat ggtctggctt
    27421 cagcgagaag ttccaacgca tatacaagga cgacttatgt caacgataac aacgattacc
    27481 atggtggcca gtccacttgg attagcactg tttggtgcgc tctttgattg gaagacaacg
    27541 caaccacgtc tgcgagacag tctaattttt ctgggaggga gtcttgctat agttatcctt
    27601 ataaccggtc tggtaaaatt actcaagctt gatctgaaag aagcaaaaat tttcagtgaa
    27661 gaggaataca gagaaacgac tttggtcgaa aagaaagaat caggaaactg atacaagcgt
    27721 ataacaaact gcctgataat aggaaagaca cgtattgtcc gattgttttt tttgacattc
    27781 aattggaaaa tttacacttt ctttatacat ttctggctgc tttctttata cccgtcaact
    27841 atacttcact catcaataag aaagaaggat aagtatggat tttggaaatg aaacggtctt
    27901 gaaagcaact ggtataacca aaaaatacgg tgctgccaaa gcattagata aagtatcaat
    27961 cgaaatcaaa cgtggaatga tctatggatt gattggggaa aatggagcgg gtaagtctac
    28021 ctttatgaga actattatgg gtttgatctc gattgatgaa ggttcgatcg aattatttgg
    28081 aacgacggat ttgcaggcag caagacgacg tatgggtcaa tcgattgaaa cccctgctct
    28141 ttatccagaa ttaactgcaa gagataacct acgtatccaa gcagcgaacg gcggtgtgtc
    28201 ggatagagaa atcgaagacc tgctgaaaat gatgcgattg gaaaatacag ggaaaaagaa
    28261 agcaaagaat ttctctttag gtatgcgcca gcgtttagct attgcaaatg cactgatcac
    28321 gaatccagaa tttttgattt tggatgaacc gacgaatggg atggatccag cagggatggc
    28381 agaaatgcga gaaatcatcc aacgtttggt aaaagaacgg ggcatcaccg tcttgctttc
    28441 tagccacttg ctagatgaat tgtcacagat cgccactcac tatggtattt tgcatgaagg
    28501 acacttgatc aaagaattat cgaaagaaga attggcacaa gaatctcgtc aatttataaa
    28561 aattgacact tcggcgacgg aacaagcagt aacggttctc gactctcttg gttataggga
    28621 ttattttgta caatctagcc gtgttatcca gctttttgaa ggaattgacc aagtagcagc
    28681 catcaatcaa gcattggtag aagcaaaagt tcctgtggat ggtattcacc tagttgggca
    28741 aaaactggaa gattatttct tacaattaac tggaggcaat ccacatgttt aatctattaa
    28801 ctgctgaaaa aataaaagta tggaagagcc gtaaaatgtg ggtctcacta ggactgatgg
    28861 tcgtacttcc gcttttttat tcttggaacg aatggtatat gcatacaaaa tacggcaatg
    28921 aattaaacca agcaacggat acagtcatca acggtgcaac agggatttta atggtagaaa
    28981 aaatggctgc atggattctt ctggctttcg cagcatttgc gtgcttttat attggggaag
    29041 aatttcaaaa cggtacgatt cgcaatacgt tatctttagg acggaatcga atgacttatt
    29101 atgtgtcgaa acttctcgtt acgcttttga tcacaacact tggcgtggtt ttgatcacgg
    29161 gactagccat gcttgcttac acacttgctt tcggatttgg tgaagttgag ggaatcaaaa
    29221 actatggaaa ttatgttcta aaagtatttc ctgtattgct tctgctgaca ttagggacat
    29281 tatctgttcc agtggctttg acattcatca ctagaagtac cagtgtcagt ttgcttcttt
    29341 cttttcttta tatcatggga acggccttca ttcccggtgt tttcgcaaaa atcaaaggct
    29401 tggaatttct aacggagtgg tttacggaaa cgtggctcat gtatacggac tttgcgcaac
    29461 aagcaactta ttcgcaagca ccgaaaatga ttctggtcag tctagtaaca atcgtggtat
    29521 catcagctct aggaatgttt attttccaaa aatctgacat taagtaggtg gtcctgctgg
    29581 aaactatttt gattatcgaa gacgatgaag ctgttcattc cttactggag gaggttcttg
    29641 aacagagata caaacttctt gatgcttatt caggtacgga aggaaaatta ttgttaagca
    29701 catatcctgt cgatttgatc cttctagatc tcatgcttcc agggctttca ggtgaagcat
    29761 tgttagcaga aatccgacag acatcgaatg ttccgatcat cgttctttca gcaaaaagcg
    29821 atcaaagaga taaagtttcc ttgttggcag caggagcgga tgactatgtg acgaaaccat
    29881 ttgatatcga agaacttttg ttacgaatcg gcatccaact tcgtcatcag acttctgtcc
    29941 aagtaaaaga tctgtcgcaa cgcatctatt acaaggaaat cttactggac aaagagtcga
    30001 gggatgtaaa tgtgaatggt gtggctgtcc atttgacagg aagagaattc gatctgctga
    30061 agcttttttt agaacatccg aaaaaagtat tttcgcgagc aaatctttat gaaacagttt
    30121 ggcaagaacc atattttgat agtgagaaaa cagtgaatat gcatatttcc aatcttcgag
    30181 ggaagctagc aaaggcacat gctgtgtata ttcatacggt gtggggagtg gggtttagat
    30241 ttgattaaat ggctgttgct tctcgttgtt atctttatgg gaagttttat tttcttcctg
    30301 caaagggaaa tcaagagatt aactaagaaa atcgccactc tttctgaacg acaagaatat
    30361 ggtggtcgtc tttcagtcga atttcgagaa aagaatctaa tggacctagt agatgcactg
    30421 aatacatggt tggatgaaaa tgaacaaaaa ggacaagcat ttcaagaaat ggaagaaaac
    30481 atcagactat cgatcgtcgg cctttctcat gatctcagaa ctcctttgac tgctattaac
    30541 ggttatgtac aattactgga acaggtagag gatacggaga aacgggagaa gtatttaacg
    30601 atcatcaaac aatctgttgc aagattactc agtatgacgg atcagtttta tgatcttgct
    30661 cgaatagaga cgaagcaaaa agaaatggat ctccatacgc tttctttttc taaagaggtg
    30721 gaagaagtat ttttctcttt ttatgaacaa ttttctgaga aaaatatcga ggtagcgttc
    30781 gctgaacact cgataaatac aactgtatta gcagatccgc tcatgttgct gcgagtgctg
    30841 caaaatatca tacagaattt attgcgttat gcagatggac aagcaaaggt gacattttca
    30901 aaagaggatc cattcattcg attaacagtg gccaatccta tcaaaccagg aacgcaaacc
    30961 tctgttgaaa aagtcttcca gcgtttttat accgaaaata ccagccgcac aaatacggaa
    31021 tctagcggaa tcgggcttta cctgtcaaaa aaattggtgg aaggaatggg aggagagatg
    31081 accgctagat tagacggggg catcttctcc atctccgtca agttgcggag ggtttaatta
    31141 tacaagatta gtgagaacta gtagaaaaat gtattatctt atatatattt aatagagaaa
    31201 aaagcctgtg atataaatat tgcaggcttt tttattgatt tgaaataatc aagaatatta
    31261 gaaacttcta cattaatcaa gaactttctt ctgtatgaaa atagagataa taattgtaca
    31321 cttaagctgt attcaggagg tgaagataat gggcatcgaa tttagcgaac ggcaaatgaa
    31381 aatactatac tttttgattg aaaatgagca cacaacttac aaagaattat cagaacaatt
    31441 atatgtatct acgaaaacaa ttagcaaaga tattttaatt ttgaaagaaa taatccaaaa
    31501 aatgccagat gtacaaatgt tggttcaacc taaaaaaggc gtttcgctta ttggaaaaaa
    31561 agaaacactt cgaaatttat tatccagtca taaaatcaat atcgcagact ctccggagga
    31621 ccgtctaact tatgtgtttg ctaaattatt gaatactaat gattctctaa agattcaaaa
    31681 tcttgctgac gaattatacg tgagcaggtc gacggtagaa agcacaatga aagatgttag
    31741 aaaaagacta aacaagcatg ggatgtcctt agtttcagat cgaaatggcg tgcgcatcaa
    31801 agctgatgaa aaaagtaaaa gaaggttgat gtcagaagtt gtgaactatt attggggtgg
    31861 ctttacagca tctaacagta aaacagatct cttagacttg aatatcaatt tcattggtga
    31921 agcgaataat ttcttaaata aggacatatt gaaaaaagta gccgaattgc ttaatcgata
    31981 catgaataag acacaattga aatttacaga ttatgaattt caaactttag ctatccacct
    32041 tgctattgcg atagaaagga ttgaaacaag caatttctta tcacgtagta ataattctga
    32101 agaagaaatc tcaaagaata cacttatttt aatcaaaatg attgaaaaag cattttcaat
    32161 tattgtgcca atagatgaac aagcttatgt tgacattcac attacttcga ttgaaaaaaa
    32221 tgctatga
//
LOCUS       DASJUF010000022        31696 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJUF010000022 DASJUF010000000
VERSION     DASJUF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.022"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1018..1359)
                     /locus_tag="U4B62_000918"
     CDS             complement(1018..1359)
                     /locus_tag="U4B62_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2141093.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1359..1589)
                     /locus_tag="U4B62_000919"
     CDS             complement(1359..1589)
                     /locus_tag="U4B62_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2141094.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1876..2214
                     /locus_tag="U4B62_000920"
     CDS             1876..2214
                     /locus_tag="U4B62_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141095.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2419..2658)
                     /locus_tag="U4B62_000921"
     CDS             complement(2419..2658)
                     /locus_tag="U4B62_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2141096.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2634..2981)
                     /locus_tag="U4B62_000922"
     CDS             complement(2634..2981)
                     /locus_tag="U4B62_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2141097.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3713..4294)
                     /locus_tag="U4B62_000923"
     CDS             complement(3713..4294)
                     /locus_tag="U4B62_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2141098.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4477..5103)
                     /locus_tag="U4B62_000924"
     CDS             complement(4477..5103)
                     /locus_tag="U4B62_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2141099.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5125..6456)
                     /locus_tag="U4B62_000925"
     CDS             complement(5125..6456)
                     /locus_tag="U4B62_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN2141100.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6611..7012)
                     /locus_tag="U4B62_000926"
     CDS             complement(6611..7012)
                     /locus_tag="U4B62_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2141101.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7098..7304)
                     /locus_tag="U4B62_000927"
     CDS             complement(7098..7304)
                     /locus_tag="U4B62_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141102.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7399..8901)
                     /locus_tag="U4B62_000928"
     CDS             complement(7399..8901)
                     /locus_tag="U4B62_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN2141103.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8919..9374)
                     /gene="argR"
                     /locus_tag="U4B62_000929"
     CDS             complement(8919..9374)
                     /gene="argR"
                     /locus_tag="U4B62_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2141104.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9472..10089)
                     /locus_tag="U4B62_000930"
     CDS             complement(9472..10089)
                     /locus_tag="U4B62_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN2141105.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10310..11683
                     /locus_tag="U4B62_000931"
     CDS             10310..11683
                     /locus_tag="U4B62_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2141106.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11758..12870
                     /locus_tag="U4B62_000932"
     CDS             11758..12870
                     /locus_tag="U4B62_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN2141107.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12942..14579)
                     /locus_tag="U4B62_000933"
     CDS             complement(12942..14579)
                     /locus_tag="U4B62_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN2141108.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14747..15355)
                     /locus_tag="U4B62_000934"
     CDS             complement(14747..15355)
                     /locus_tag="U4B62_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2141109.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15739..16890)
                     /gene="queG"
                     /locus_tag="U4B62_000935"
     CDS             complement(15739..16890)
                     /gene="queG"
                     /locus_tag="U4B62_000935"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN2141110.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16909..17661)
                     /locus_tag="U4B62_000936"
     CDS             complement(16909..17661)
                     /locus_tag="U4B62_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141111.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17802..18752)
                     /locus_tag="U4B62_000937"
     CDS             complement(17802..18752)
                     /locus_tag="U4B62_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN2141112.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18753..19592)
                     /locus_tag="U4B62_000938"
     CDS             complement(18753..19592)
                     /locus_tag="U4B62_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2141113.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19589..20335)
                     /locus_tag="U4B62_000939"
     CDS             complement(19589..20335)
                     /locus_tag="U4B62_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2141114.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20573..21943
                     /locus_tag="U4B62_000940"
     CDS             20573..21943
                     /locus_tag="U4B62_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2141115.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21998..22894)
                     /locus_tag="U4B62_000941"
     CDS             complement(21998..22894)
                     /locus_tag="U4B62_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2141116.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23008..23178)
                     /locus_tag="U4B62_000942"
     CDS             complement(23008..23178)
                     /locus_tag="U4B62_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141117.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(23175..23852)
                     /locus_tag="U4B62_000943"
     CDS             complement(23175..23852)
                     /locus_tag="U4B62_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2141118.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23943..24848)
                     /locus_tag="U4B62_000944"
     CDS             complement(23943..24848)
                     /locus_tag="U4B62_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2141119.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            25023..26477
                     /locus_tag="U4B62_000945"
     CDS             25023..26477
                     /locus_tag="U4B62_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2141120.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            26493..27524
                     /locus_tag="U4B62_000946"
     CDS             26493..27524
                     /locus_tag="U4B62_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2141121.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27531..28718
                     /locus_tag="U4B62_000947"
     CDS             27531..28718
                     /locus_tag="U4B62_000947"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN2141122.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28874..30256
                     /locus_tag="U4B62_000948"
     CDS             28874..30256
                     /locus_tag="U4B62_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2141123.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30383..30613
                     /locus_tag="U4B62_000949"
     CDS             30383..30613
                     /locus_tag="U4B62_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141124.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(30813..31481)
                     /locus_tag="U4B62_000950"
     CDS             complement(30813..31481)
                     /locus_tag="U4B62_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN2141125.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8978 a         6421 c         5711 g        10586 t
ORIGIN      
        1 ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa
       61 tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca acatataccc
      121 tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt
      181 ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc
      241 aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc
      301 tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat
      361 caattagaga ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga
      421 ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg
      481 tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg
      541 tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg
      601 cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa
      661 attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt
      721 agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc
      781 gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa
      841 cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt
      901 aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct
      961 ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta
     1021 aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat
     1081 ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc
     1141 ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt
     1201 aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag
     1261 tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat
     1321 atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca
     1381 ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat
     1441 ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat
     1501 tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt
     1561 ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat
     1621 ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg ttttgtttaa
     1681 agtatatctt attggatata aatagaaata aattgtagcc agttcatttt ttctttaact
     1741 aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa atactatagc
     1801 taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca
     1861 acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt ttgctctgac
     1921 aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac tgttcggatc
     1981 aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag acaagacttt
     2041 gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt acatgatcca
     2101 actcatatca atgtggataa atggcaacct cttatctata aattcagaga atatacaact
     2161 gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc ataatgataa
     2221 acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa tagaagctat
     2281 acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat gagcatttct
     2341 tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg ccgctctttc
     2401 tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct gctgagtaat
     2461 gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt cagtcaagac
     2521 aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag tcattatgat
     2581 tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat acactatttt
     2641 ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcattgg gaaaacacaa
     2701 cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca
     2761 ttaatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg
     2821 aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca
     2881 gctgctaatt ctgctgcctg cttcggttgt ttcgtctggg cttctcgata ataattctga
     2941 aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaatctt
     3001 tttttctatc atacactgca acaagaagtt cgcaccaaca aaacgctttc aatttttgta
     3061 gaactaaaaa agttgtgaca agtgacaaac tctccagtga atggtattct cctgccaact
     3121 cctcggtcac aagtcacaat attcaacaaa catgagaagc tgctttttga aattgttcct
     3181 tgtgactcgg agtgaaacgg caggcttacg acctctgggt aaacggtgtt caaaagccag
     3241 tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gctattttca caaaattttt
     3301 cttaatactc gaaactaagc gactttttca caacctcttc cttaaacggt gttcaaaagc
     3361 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agcagctatt tttatttggc
     3421 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctccactaaa cggtgttcaa
     3481 tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     3541 tgtttctcta atactcaggt caaagcgctg atttcacaac ctctaataca cggtgttcca
     3601 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
     3661 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa
     3721 ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata
     3781 gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg
     3841 cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt
     3901 tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca
     3961 ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct
     4021 catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag
     4081 catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg
     4141 ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag
     4201 aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt
     4261 tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat
     4321 agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt
     4381 gccaaattat ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt
     4441 gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc
     4501 ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc
     4561 ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac
     4621 caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt
     4681 gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt
     4741 atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc
     4801 ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc
     4861 aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata
     4921 atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat
     4981 attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc
     5041 tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa
     5101 catggtggat tctccattcg ttatttattt taatacatca tttagagctt cactcattgt
     5161 cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag
     5221 cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa
     5281 gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc
     5341 tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc
     5401 ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac
     5461 agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg
     5521 ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca
     5581 aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag
     5641 tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc
     5701 caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc
     5761 tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc
     5821 atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc
     5881 agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc
     5941 caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat
     6001 cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat
     6061 tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt
     6121 attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc
     6181 agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc
     6241 tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt
     6301 agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac
     6361 gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc
     6421 aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct
     6481 accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta
     6541 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
     6601 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
     6661 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
     6721 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
     6781 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
     6841 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
     6901 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
     6961 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
     7021 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
     7081 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
     7141 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
     7201 ttttctacat aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc
     7261 ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc
     7321 tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag
     7381 cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa
     7441 taattttgct aattttacta acgccaactt gttctttccg aattcatcat tttctatctg
     7501 taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg
     7561 ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc
     7621 ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc
     7681 gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg
     7741 ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat
     7801 gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc
     7861 gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt
     7921 tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag
     7981 atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat
     8041 tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag cactgacaac
     8101 tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac
     8161 agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa
     8221 tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac
     8281 tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt
     8341 tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc
     8401 ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg
     8461 ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa
     8521 ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca
     8581 cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc
     8641 gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc
     8701 gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt
     8761 cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt
     8821 caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg
     8881 cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc
     8941 ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca
     9001 ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca
     9061 tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa
     9121 acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta
     9181 aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga
     9241 atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc
     9301 agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc
     9361 tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt
     9421 tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt
     9481 ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct
     9541 gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc
     9601 ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc
     9661 acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt
     9721 gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat
     9781 catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg
     9841 cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca
     9901 atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg
     9961 tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg attttttagg
    10021 aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat
    10081 tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt
    10141 tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt
    10201 aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta
    10261 taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca
    10321 aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt
    10381 attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac
    10441 gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc
    10501 gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct
    10561 gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt
    10621 tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat
    10681 cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac
    10741 ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg
    10801 gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta tcggctggtt
    10861 tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg
    10921 aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag
    10981 ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa
    11041 agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt
    11101 gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt
    11161 ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc
    11221 agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa
    11281 gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact
    11341 cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat
    11401 cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca
    11461 aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc
    11521 attagtagcg gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc
    11581 tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt
    11641 tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg
    11701 tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg
    11761 gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat
    11821 acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc
    11881 gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct
    11941 aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt
    12001 tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt
    12061 cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct
    12121 gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt
    12181 ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa
    12241 caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc
    12301 aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt
    12361 aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc
    12421 ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga
    12481 gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac
    12541 gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac taagagtaca
    12601 gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt
    12661 acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc
    12721 ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat
    12781 ctattagcca acttcttaaa tactggtcat tggcctacgg aagattatca aaaggacatc
    12841 tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga
    12901 gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc
    12961 aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca
    13021 atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca
    13081 aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac
    13141 ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg
    13201 atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa
    13261 aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca
    13321 aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat
    13381 cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata
    13441 attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga
    13501 tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt
    13561 atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga
    13621 caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag
    13681 taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca
    13741 tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca
    13801 gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc atattgtttc
    13861 atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa
    13921 gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga
    13981 gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag
    14041 atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa
    14101 ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc
    14161 agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa
    14221 aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt
    14281 tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca
    14341 tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt
    14401 gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttccta
    14461 tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg
    14521 gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg
    14581 gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt
    14641 atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa
    14701 cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt
    14761 ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt
    14821 ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt
    14881 gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg
    14941 cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag
    15001 ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg
    15061 tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg
    15121 ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag
    15181 caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat
    15241 cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg
    15301 cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa
    15361 actccttttt ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata
    15421 tttcataggt aaaagaaaca tgacatttaa tgacaaaata acgaattatt taatatttgt
    15481 aacatttaaa caaatatttt tttgatacaa aaccataaaa gccttgttgt ataagcaaag
    15541 taatcgtttt tctatgtttt tttatttttg aatgaaaaac gcagattctg taacattcac
    15601 tgcaataatc cataaaaaaa agcaaaaaaa tttttgattt ttatcaaatt ctcttaaaaa
    15661 aatgtaaaaa atacaaaaaa accagtaatt gttacaacaa ttactggaca aaaactttga
    15721 ataatgttaa ctattttttt attttaactt tctccgcaat aatttctggg catgttccaa
    15781 ttcgtttata acgacttctt ctgtttcttt agctaatatt tcatctaata catcaagcca
    15841 tttttccggt tcttttgttc ctagacgtcc aatagcccaa gctgctgttc ctctgatgac
    15901 agggcggaca tctgttaaac attctgaaat ctcaggcaat gccgttcgat cacctagatt
    15961 agctaaagca atcaatgcgt ttctctgcaa aggtttcttc ccacgccatg aaccagctaa
    16021 atgcccaaat tgctgtttga agtctttatt agacatcgaa agcattggct ttaattttgg
    16081 atatatttcg tctacttttg gttccatctc ttcatgaaaa tgaaaatctt ttccttgatt
    16141 gtaagggcag actaactggc agatatcaca tccataaatg acatttcgca tttttttacg
    16201 atattcttcc ggcatcatcc cttttgtctg agtttgataa gacaaacatc gctgggcatt
    16261 cattcttccg tcaccaagca atgcttttgt cggacaagca gtcacgcagc gcatacagtc
    16321 accgcaacca aattctcctg gtttatcagg ctcaaataca atattagtaa cgatctcacc
    16381 aagatagaca aatgaaccga actcttcagt gatcaacaat ccgtttcgtc caataaatcc
    16441 taaaccagct cgctgtgcta cagcgacatc gatcagttct ccagtatcta cttgcggagc
    16501 tagacgccat tcctcttctt tttgccattt ttctgctctt gtttggatga attcgatcaa
    16561 tcggttcaaa cggtctctga gtatatcatg gtaatcgatc ccccaagaag cacgagcaaa
    16621 ctgcccgcgt ttttcatccc taggcacttc ttcatggatc ctagtaggat acgcaagtgc
    16681 aatggaaatg atcgatttcg gctgatcaaa tgtcatttct ggataaagac gctcatcgat
    16741 atttggatgt tcaaagccag aagtatggcc agctgctttt tgttcttcca acgattcttt
    16801 taatgaatca aaaggttccg ccgtggtgaa cccgattttg tcaattccta atcgtttgct
    16861 ttcttgtatg atttcttctt ttaatgtcat ggtcttcctc gcttgctgtt aacggctgta
    16921 taacccataa tatagttttt cttttttcag ctggacaaac tggatctgct gatcggcaat
    16981 cttcttttct tccatccatt tttttcttgc agcaaatact tcccaataat ttgttgaaat
    17041 taattttctt ttgttatcta atgcattaaa aaaattcaat gaaactggtt ttgtacttaa
    17101 cagttcatgg agccttacct cttcccatgg cgaaaactca gacagatcgc gggaatacaa
    17161 tacttcttca tagagatttt tttgctgtgt gtacgtgaat gtttgcggga ttttcccagg
    17221 cctagcaatt tctactagac gattataaaa catattcatt aatttgctgt tggcaataaa
    17281 ttgactttta taataggccg actggatctt tttgattact ccttctgctt tgatgactga
    17341 aaataatgca ggccatgctt tgacggttat ttcgtcgttt tgtttaacta atacgccagg
    17401 tgtgaagccg actggagaaa ggaacacaaa ataaccgata ccggtatttt catctaaagg
    17461 ctgaaattca tcaaacaacc atttgcctat gtacagctct tcatctggtt ctgttgtctc
    17521 attttgattt gctatgaata atttagctaa ttctgcttca agaaattgaa ggtcttgatc
    17581 cttgtcggta ttttcctgct ggctgatcaa tcgaaagact tcttctttaa tcgtatattt
    17641 atctttacgt gtaaaattca tagccacacc ttccttcctt actctaacta taacggaatt
    17701 tcttcaaaaa aggtatctgt acgacgaaaa acagcataat tttttttcat atctcttata
    17761 aaaaatgcaa ggtctgctcc tcaagctaga ccttgcactt ttcagctttc ttttgtttgt
    17821 gacaaacctt catggatcgt ttcaatattc catttcatca tctgatagta agaatctcct
    17881 tgttctcctt cttttgctat ggaatcagtg aacagtttat catagatttt caaacccgtt
    17941 tcttttgcta cccgttccat gcttcttgag tctacactgg tttccacgaa taaaaccggt
    18001 acttcttttg ctcttatctg atcaatgatc gctttcattt gatcaggcgt accttgactt
    18061 tctgtattga tctcccatat ataagctgct ggcaaatcat atgctcgtga aaaatacttg
    18121 aatgcccctt cacttgttac taataatttt ttgttctctt cgatagaagc aaaactttcc
    18181 ttcgcttgtg tatctaattc ttttagtttt tccacatatg cttctgcatt ttttttatag
    18241 agtgtcgcat tttctgcatc tttttcagag aatatacgtg cgatttcctc cacatatttt
    18301 attccgttag ataggtctag ccaagcatgg ggatctgctt ttgtatgttc ttcaccgcta
    18361 gttaaataaa gaggttctac atttttgcta actgcaaaat aatcttttcc ttcttctttt
    18421 ttagccgttt ccatcaagtt atcgaaccag ctgttacctg tttcaagatt caaaccgttg
    18481 tataaaataa catctgcatc gcttgccttt ttgatgtctt ctggtagtac ttcatattca
    18541 tgcggatctg ttccgactgg tacgatactg tggatatcta tttgatctgt tccgacctct
    18601 tttgccatgt ccgccaagat cgaattggta gccacgactg acaacttttc tttacttcct
    18661 tcatccgctt ggtcagaagc ctgctttccg caaccggcta ataccaagaa actaactgcc
    18721 aagattaaaa agagtgattt ttttgttttc atctatctgc ttcctttctg aaataaaatt
    18781 ccttttgttg gagaaaataa aaaagcaagg ataaaaaatg cggtacatgc taacacgatc
    18841 gtagctcctg atggccaatt gtaactataa ctgaaaaaca caccgacaac tgcacttagc
    18901 ataccgattc ctgcagctgt aatgatcatt ttcagtaaat ggttcgttag caagtaagcc
    18961 gtggcggctg gtgtgatcaa catggcaatc accaagattg ttcctactgt ctgtaaactg
    19021 actacagcca ctaatgtcaa aaagaacatc aaaagataat ggatcagcga tgtgttcagc
    19081 ccataagctt ttgccattgt cggatcgaat gatgtgatct tcaattcttt ataaaagaaa
    19141 aagacaaata tcaagacgat tccactaacg agtgctgtca gcaacaaatc gctttcccta
    19201 acagctaaga cattcccaaa taaaatatga tacaaatctg tcgcactctg ggcatatgaa
    19261 atcaagataa tccctaatgc aaaaaaggag ctaaagacaa ttccaatcgc tgtatcattt
    19321 ttcaatcggc tccgctgagt cacgaaacca atcactgctg ctgttagcat cccaaaagca
    19381 gtggctccaa agatgtaact aaaaccaaac atataagaaa tggctacacc tggaagaact
    19441 gcgtgagaga tagcgtctcc catcaaagac atccctcgta aaataataaa agaaccgatg
    19501 actccagaag caagtcctac aagcatggaa gtgaccaaag cgtattgcag gaattgataa
    19561 ttcattaatc catcaataaa ggattggatc attttttctc tcctcctttt actaaatgtc
    19621 tgatgatctc accataggcc aattgaaggt tcttcgatgt aaagacttct tgtactggac
    19681 cggaagcgat cagctgttta tttaaaagaa taacctcatc gaaatattct tctactttat
    19741 gaagatcatg atggacgatc aaaatcgttt ttcctgaatt cttcaattcc tgcaagatgt
    19801 caaagatctt tcgttcactt aacgcatcaa tccctacgaa tggttcatct aaaaagatcc
    19861 attctgctcc ttgggctaga gctcttgcaa taaaaactct ctgaagctgt ccacccgata
    19921 gttcgctgat ctgtcttttg gcatattctt ccatccctac ttttttcaaa gcttgtcttg
    19981 cacgttcttt ttcttttttc ccaggtcttt gtccaattcg taaagatgga tatgttccta
    20041 aaagtacaac gcctaataca tcaattggaa aggaaagatc caattcactt cgctgttcca
    20101 cgtaggctat ttttttacgg actgagttta ccgactgtcc agaaaaggtg actttaccag
    20161 tagtagctgg tatcaactct agcaaagatt tcataaatgt tgattttcct gcaccgtttg
    20221 gtccgataat accggtaatt ttttgtggat ttatcgtcaa gttgatagtt ttcaaaacag
    20281 ttttcccttg ataagcggct gaaaggtctt gaattacaat ggccgtacgt tccaaatctt
    20341 acactccttt ttaggtttac ctaacttttt atttaaatat agtttaacta aaatggaaag
    20401 cattttcaag tagaatgatg aaaaagtttt tttcgaattt tcctcgcatt ttatgaaaat
    20461 tttctgtaga atcacatcaa gagttcaata aggctaacct aataatgaaa aacatgtgtt
    20521 gaaacgtttg attatttttt cttgttgact caacaacgaa aggagttttt gtatggaaga
    20581 agaaaagtta actcgaagtc ttagtgcccg tcatatccaa atgatcgcac taggtggaac
    20641 gatcggtgtc ggtctgttta tgggtgcctc ctcaaccata cgttggacag ggccctcagt
    20701 gatgggagcc tatgcgattg caggattctt tttgtatttg attatgcgtg ctttaggcga
    20761 aatgctttac gttgatcctt cgactggttc ttttgcaaat tacgcgagcg agtacatcca
    20821 tcctgtggct ggctacttga ccgcttggag taatattttt caatatattg tcgtaggaat
    20881 aagtgaagtg attgctgtag gatcttatat gaattattgg tggcctgatt tgccggcaat
    20941 tattcccgga atcattgtcg tcttgtttct tatgctggca aatcttattt ctgtcaaggc
    21001 gtttggcgaa ttagaatttt ggttttcgat catcaaagtc ataacgatca ttttgatgat
    21061 cattgcaggt cttggggtaa tcttatttgg atttggtaat catggtcagg cagtagggat
    21121 ttcaaatctt tggaaaaatg gtggattctt cactggaggc gtcaaaggat ttttctttgc
    21181 attatcgatc gttgttgctt cctatcaagg aatcgaactg atcggaatga ctgctggtga
    21241 agcagagaat cctaaaaaga cgatcattga agctgtccag tccactatcg gtcgaatctt
    21301 gattttttat attggtgcta tttttatcat tgtgagtatc tatccgtgga atcaattgag
    21361 tgagattggt tcaccttttg tacaaacttt ttctaaagtc ggcattacat ttgctgcagg
    21421 actgattaat tttgtcgtga ttacagcggc attatcaggt tgcaattcag ggattttcag
    21481 cgcaagtcgt atggtctata cacttgccat aaatggaaag atgtctaaaa aatttctgaa
    21541 actatctcgt cacggtgcgc ctttttatcc tgtcgtagca atctcaggtg gaatcttaat
    21601 tggtctgatc ttaaattatc ttctaccaat gttcatcaac cagtcagaaa atattttcgt
    21661 tttcgtatac agctcaagta ttttgcctgg tatggttcct tgggtagtga ttttgatcag
    21721 cgagattcgc ttcaaaaaga agcacgcaga taaaatgcat gatcatcctt ttaaaatgcc
    21781 actatctcct tatacgaatt atgtgacact tgctttttta gctatcgtat tgattttcat
    21841 gtttatcaat cctgaaacga gaatctcatt gattattgga ctcgttttct tgctttacat
    21901 gacgatccat tactttgtac gcgaaaaaag aaccggaaaa taaaaaaaga acgaagcttc
    21961 accttagtgg aagcttcgtt cttttttatt gcttttatca attcaatttt agcgtctcac
    22021 cgggagtcag gaaataattc tccatgctca tcccattcag cgacaagatc gtttctactg
    22081 gtacgccagt tcgttcggct atttgatttg gaccttctcc ttccaaaact acggttgtcg
    22141 cagcctgtga agtcgcgctg ttggtattag tttcagagga atctgttgtg tcttgtgttg
    22201 ttcgagcttg catttcagaa gcttctaccg actctgctgt ctcagaatca gaagatgttg
    22261 tgtcttgtgt gcttgttgac tctgcttgaa gagaggcgct agtctgcaaa taaatatcgg
    22321 aaatcttagc gacatgattc gtataattag ccgataattg attgatcaaa gtatctaagt
    22381 ctacaccgtc tttattttct gctgcctgat acgctgcacg taattttctg atcccttcgt
    22441 cggagattgc ttgaagctca cgtgcttttt ggttagcaac tgctgataaa ccagctattc
    22501 cctcttgatt tccctgaatt tctgtctggt attcttcaat caatgcaggc gtcttttctt
    22561 cgatcttttt cgaataatca tttacgattg cttgaagttg ctctggcaaa ccatttgctg
    22621 ttttttcttc agaagtttgt gtctcttttg ttttagttga tgactcactt gtttgtttcg
    22681 tctctgtttt ctttgtcgtt gtttccgtta tggtcgtact tttcgcttct gacgaatttt
    22741 ttccatgaaa atatagaccc gcaactcctc ctgctatgat caaaaacacg gcaactgctg
    22801 ccccaatcat gatcatccgc ttacgagctg cctgtttttg ctgtcttctt cgggtcatcc
    22861 gaacattttc tccccactca ttttcatcat acattttttt caccaccaat ttttatgtat
    22921 aatagcgtta tcaaacgctt catattctta ttgtagcaaa taattgtcgc tgtgcacatt
    22981 tttatctttg ttatttataa gaaaatttca ccagttgaga tgattccaag attgtccgtc
    23041 gatttctcct tgcagctgca ttgttcgttc gtctatctca gccatcagtt tctttatttc
    23101 atataagagc gcacggtctt gatattctga tttttcgctt aattcatcta attgttggat
    23161 cagccaatca tttgtcataa tgaagttcct ttcatttctt gcagtgtaaa ggtcaagtct
    23221 tcgatctgct cttttgcctc agtcagtgct tctacgatat atgcttgttt ttcagattct
    23281 tcttcctgca ttttctgatt ttcctgtact ctttgaataa accagtcaat ccgctgagtc
    23341 agcgatggtt tgccttccaa tgagagatac tgctgataag caattagctt ctcccaacgt
    23401 ttttcctggg ataaatacat aaattgtgct aaaacgaatg gcggaagata ggtcatatgg
    23461 aatttatttg cgattcgctg atacggcctc aaaaattcgg aaatcgtata ttcttctttt
    23521 cctccagctt ggtattcttt ttccgccaca cctgttgtca atacgattcc tagttctttc
    23581 ccttttaata catgtgctgc tcgttccaag acattatctt gccaaatttt taaatgagca
    23641 ggagaactat accagtaaaa cggaaactgg aaaatgatcc gatcatgctg ggccaagagc
    23701 tgtagttcag tctctatatc tatttttccg tctggataac ttgattccaa atgatgccaa
    23761 gtagctggta aaccagcaga agcttctttg aaaaattgct gatttaaaga ttcttcgatc
    23821 gttggatggc taatgatgat aagtgtacgc atcccttttc ctccttttta gtcttcgctt
    23881 tacagtgtag cataaccgcc acaagtttct tttgccgatg cttgtcattt taaccagact
    23941 aatcaggttg tccaagaaac tgcggtttgt ttttccaact gttttttcaa ggtatccaaa
    24001 gcaatattct taaaattttc gtttttcata cgtttacgat agttcgtagc cgtttctccg
    24061 gtactctttt tgaacattcg gctaaatacg actagactat tgaaccctac ttgataggca
    24121 atactttcca cggattcatc cgtcagagaa agaagaaacg cccctcgatt gatccgtacc
    24181 ccgatcaaat attccttgat actggtaccc atatgatttt tgaatactcg cgacaaatgg
    24241 ctccgattga ccgctaaatg ctgggcgatt tcctcaacag acaatttttc agcataatgt
    24301 gcctcgatgt atttgactgt ttcaacagct aaagaggaat gaaccttttg tacaccaccg
    24361 ctgacctttc cgccatcttt taaaaggact gataaaaaac ggtaggttaa tgctgttaac
    24421 tgtaattcat tttccaaggt ttcttccgta tctttcatgc attctaaaat aatgtcgata
    24481 tatttttgta tgtcagaaga gaccatcgtg tattggtctt ctctgaaaag cgactgccga
    24541 atgatttctt ctgcagcact cccaccaaat gaaagccagc aatacatcca cggatcttct
    24601 ccatctgata agtaaaacgt cgagtctcct ggacgaatca agaacagatc accttttttc
    24661 aattggaatt gttgattttt cacatgataa gatcctttgc cttccatcac gacatgcagg
    24721 atgtaatcat tgcgaatagc aggaccaaaa gaatgaaacg gcatcgtttt agagaaacca
    24781 caaaaatcaa aatacaattc tttttctact tgttgatgtt ccttttttaa aaaaatcgat
    24841 tgctccacgt ctatccctcc ttactccttt agtataatgc aacaaaaagt taaataaaag
    24901 tcacagaaat atattaaaaa ggaagcgatt tcttgttaag tttaagtcat taaaagatct
    24961 ttgttattcg ttgtaaccgg tttacttagt attatatatt gaacaggaga ggaatacaga
    25021 ttatgaaaag caaaatgatc caacgtgtgt cctacgcttt aggcgctttt ggacacgaca
    25081 cattctatgt tacattaagc acttatttta tggtattcgt gacaagccag atgttcgcag
    25141 gaacagataa ggcaacgaat gcaaaaatga ttgctacagt tacaagctta gttgtaggta
    25201 tccgcttagt tgaaattatt tttgatcctt tgattggtgg catcatcgat aatactcgta
    25261 caaaatacgg taaattcaaa ccatggttag ttatcggcgg cgttgttagt tccgtactct
    25321 tggtagcaat ctttaccaat ttctttggct tagctactca taatacgact ttatttatca
    25381 tattatttgc aatcgttttc atcttattgg atagttttta ttcatttaag gatatcgctt
    25441 tttggtcaat gattccagct ttgagtacgg aaacaaagga acgtgaaaaa tttgctacgt
    25501 ttgctcgttt cggctcttct ctaggtgcaa atggtactac tctagtcgtt gtccctatcg
    25561 ttactttctt tacctatctt gttactcata aacatattga aggcgcttca ggctggttct
    25621 ggttcggtgt gattgctgct gttatttcag gcggtactgc gattatcact gccttgggca
    25681 caaaagaagt cgatactgcg atccgtcagc aaaatgaaaa agctgggatc aaagatgtgt
    25741 tcaaagcagt tgctcaaaat gatcagttga tgtggcttgc ccttgcttat atcgcttatg
    25801 cgattgccaa tgcagcaaca actgccgtat tattctactt cttcaaatat gtacttggcc
    25861 aaccaactga attttggatc gttggtctag ttgctgcctt acttggttta gtagctgttc
    25921 cactattccc aatgcttaca cgcgtcatct ctcgtcgcta cgtttacctt ggagggatcg
    25981 caatgatgat ttcttcttac tttttcttta cgatcgcagg ttcaagtttg ccaatcgtta
    26041 tcatcgggct agtattgttc tacttccctc aacaattgat tttcttatcc gctttgatga
    26101 cgatcacaga ctctgttgaa tatggtcaat ggaaaaatgg tatccgtaac gaagctgtta
    26161 ctctatcaat tcgcccttta ctagataaaa tcgctggtgc tttgtctaac gggatcgtag
    26221 ggtttgtcgc tatcgcttct gggatgactg gtaatgctac tgctgctgat atcactgctc
    26281 atggcgtaac gactttcaaa gcttatgcat tctatgctcc tgctgctttg atgattttct
    26341 ccgctattat ttttgcttgg aagattacat tgacagagaa aaaacatgca caaatcgttg
    26401 agaaattgga aaacaaattg gctccagctg atacaatgga agatgaactt cacgatgcag
    26461 tagatccttc ccattaagaa aggatcttga aaatgaagat tacagaaaaa gattttggcc
    26521 aaggttatca cttgataacc ttggagaaca aaaacaagct agcactttct atctctgatt
    26581 taggtgctag aatcgtcagt ttaaaatcaa atgatcgaga attagttttg ggatttgaca
    26641 ccgctgaaga atatatcgaa aaagatccgt atatcggtgc ttctatcggt cgcaccgcag
    26701 ggagaatcga aaatggccgc ttttctctca atggaaaaac ttaccaatta gccacagatc
    26761 caaaaactgg acataatctg catggtggtg ctcctggatt tgaattaaaa aaatggtctt
    26821 atgtcatttt gaatggcgaa aatgaagctt ctgtcatttt tactactaca agtcccgacg
    26881 gagaacatgg ttttccaggt acaatggatg tggaaatccg ctatactttg acaaaagata
    26941 atatctggcg agtgacctct cgcggcacca gtgaccaaga tactttattc aatccgacaa
    27001 atcatgttta cttcaacttg acgggagatg cttctcagtc aatcgatcaa catgagcttt
    27061 ggctcaatag cgaagcttat gctcctttgc gaacagatag tattcctata ggggtaaaag
    27121 aaaatgctgc aggaagtgct ttcgattttc aaatacctaa aaaattagcg tccgtttttg
    27181 ctagtgattt ggatcaaaaa aatcttgttg acggaatcga tcatccattt ttcttaaaag
    27241 aaaccggctt gggtaaagaa gccgctcgct tgactagccc cgataaaaga atccaagtag
    27301 atattgctac agatgcttct agtgtggtta ttttcactgc aaatttcggc acagaaacac
    27361 cagaaatgcg caaccgaaaa ctggcacatc acggtggcat cacatttgag actcaaacag
    27421 ctccaggtgc agagcgcttc tcagcatttg gttcgattca tttaaaagcc ggatcagtat
    27481 ttgaaacagt aacagaattc aaaatcaaaa caagaaagga ataactcatc atggaaatca
    27541 aaagaagctt aaacacaaaa tttgccgaaa cattcggacc taaaaaaact acacaatact
    27601 tctcaccagg acgcatcaat ttgattggtg aacacacaga ttacaatgga ggccatgtct
    27661 tccccgcttc catcacttat ggaacgcaag gtgtagcagc acctcgagac gataataaaa
    27721 tattagttta ttccacaaac tttgacgata tcggtgtcat tagttttact ttagatgagc
    27781 tagaatatga taaaaaagca aactgggcaa attacgtaaa aggaatggtc cttaaattaa
    27841 aagaagctgg acactcaatc gatcatgggt ttgagttatt agtggaagga acaatcccta
    27901 atggtgcagg actttccagt agcgcttcat tagaattatt agtaggtgtt gtattagaag
    27961 atctgtttga tttaaaaaca gatcgcttgg aactagtcca aacaggtaaa aaagtagaaa
    28021 atgaattcat cggtgtcaat tcaggtatca tggatcagtt tgccatcggc ttcggtgagc
    28081 tagatcatgc aatcttatta gatacaaata cgctgaaata cgaaatggtt ccagtgaaac
    28141 tagatggtta tgcaatcgtc atcatgaata cgaataagcg gcgggaattg gcagattcaa
    28201 aatacaatga acgccgtagc gaatgcgaag aagccttggc acgtttgcaa aaaactttgc
    28261 ctatctcttc tttaggagac ttagatgaag aagaatttga atcaactatt gaccaaatcg
    28321 gcgatgatac attgatacgc agagctcgcc atgccgtttc tgaaaatcag cggactttga
    28381 aggcaaaagc tgaattagaa gctgggaact tagaagcatt tggtcaactg ttaaatgatt
    28441 ctcaccactc tcttcgctat gattatgaag taactgggat cgaattagat acactagtag
    28501 atgctgctca aaaacaagaa ggtgtcttag gtgcacgaat gacaggagct ggatttggtg
    28561 gctgtgcgat tgctcttgtc aaagaagaaa atatacctgc cttcaaaaac aatgtctatg
    28621 atgaatacat gaaagttgtc ggctatgctc cggaatttta tgttgcacac attggaaatg
    28681 ggacaacaaa gatcgatcca gaagctgttc atgtttgata aaaaaatcaa cgatctcagc
    28741 taaaaacaaa tagataaaaa taacctctca gcaatttcca aatgtgagcc ttttgctcgt
    28801 atttgaaaat tgctgttttt tattgacacg gaaagagaac gtgtgttcta attgtgttga
    28861 agaggtgatt gttatgtggg cgaatcaaaa tccggtattt gtttatgaaa aagaaccttc
    28921 aagagatatt ttatgtattg actgcaaatc tttttacgcc tccgtagagt gcgttgaaaa
    28981 ggatcttgat ccccttacaa ctaaattagt agtcatgtcc tatccgtccg attcgacaga
    29041 aacacggggt agcggattaa tcttggcttc cagtcctgca gcaaaaaaag cttacggcat
    29101 caccaatatc agccgtgcca gagatcttcc cttcccctat ccaagtgatt tatatattgt
    29161 tgccccaagg atggcctatt atatggaaaa gaacagagaa atcaattcta tctataaaaa
    29221 atatgcagac gaagcgaacc atcatgtcta ttccgtcgat gaaagcttcc ttgatgtaac
    29281 aaatagcatg gcactctttg gtaaaacaaa tgcgtacgac atggctaaac tgatccataa
    29341 agatgtgttt gagcagaccg gaatctacac gactatagga attggcgata acccgctact
    29401 tgctaaacta gccttagaca atggctcaaa agacggaaag caaatgtttg ctgaatggcg
    29461 ttatgaagat gtaccagaaa agttatggaa aatctcagat atcactgact tttggggaat
    29521 cggcaaacga acagcaaaac gtctgaaaca aatgggtata cattctgttt atgatttagc
    29581 tcacgcggat tattatcact tgaaaagcaa gctaggggtc atcggtactc agctttatgc
    29641 ccatgcttgg ggcatcgatc gaagtttttt gggacaagaa tatacaccaa aatcaaaaag
    29701 tatcggaaat agtcaaatcc tagatagaga ttatacaaga cgagaagaaa tcgagatcgt
    29761 tatcaaagaa atggccgacc aagtcggcac tcgcttgaga cgatctgcag caaaaacaca
    29821 agtagtcagc ttatgggtag gatactcttt aggctatctt gatccaaatg gcaagaccgg
    29881 gtttcaccag caaatgaaaa tcgaagcgac aaatgctagc agagatctag cagatgcttt
    29941 attgaccatt ttccaccagc attaccacta ccaggatatt cggcgtgtag gcgtgaactg
    30001 ttccgacctc gtatacgcga caggtttaca gcttaacctt tttgaagatc ctgaaaagca
    30061 gataaatgaa gcgaaagttg attttgttgt cgatacgatt agaaaaaaat acggctttca
    30121 atcaatcatc catgcgcata gtctcttgca aggaggacgc gccatcgcac gtagttctct
    30181 cgttggcggg catgcaggtg gtatgagtgg attagaaggt ggaaaagatg agacggacag
    30241 caaaaaaatt cattgactat aacgagtatc gagatcggcc atttggatta aaatggggga
    30301 ctgcttttgc gatggaagaa ctagtcaacg gtatcaaaaa aatgaagaag aagcacgcaa
    30361 acagcctctt cctaaacaac aaatgacatt gcaagaaatc gatagtatac tgacagaagc
    30421 ctttttatct cgtaaagcag taatcattca gctcaattta aaagatgaat ttggaagact
    30481 cttggatgag gtcgaagggc acttcattgg tgaagcctat gaagattact ttatgattga
    30541 ccatcagccg atattgtggg aagacgtacg gtatattgag ttaaaggaaa caaaaaaatg
    30601 gtttgtgaca tgagaggttg tgacagaagt gatcaacttc aaaaaataag aaaggatttt
    30661 tcgaaaattg cttcttaaat ttttgtggaa ttttggctta tttttcgaag aagcaacttc
    30721 tggaacaacg tatattagat atcagaggtt gtgaaaaagt cgcttagttt tgagtaacaa
    30781 gccaaggcgc tgatttcaca acctctgtct atctatccat ctattcggta agattcatgc
    30841 atgacgtctt ctttatttag tatgtagagc cctttttctt ctgaccattc aacataaatc
    30901 agctgtttta catctgcata gccttctttc ttcaagttct ccataagcca tgcttcagtc
    30961 aattgattgg actgaagcac tttatgctga atttgccctt catctacaag caggatcgat
    31021 aaagctttgt cttcatctga ttttttcaat acactcattt gaccggcatt ctctaggata
    31081 acatgtttgg cattttcaag aaaaaagcat tcttgctgtc ttaagattgc tcgcaactgc
    31141 tccatctcga tatggttatt tgtcaactct gtcatgttga tgacgccgtc tttgattaga
    31201 acagatggct cgcctttgac ccaacgattg accttttcat ttttttggac gatacgttcg
    31261 ataccataaa tcattactgc ccaaagcgca atggcaaata ggacatgccc tacatggaca
    31321 ttgtcatcat aggttgattc ttctaagatc ccacctaaaa caagcgtata gactaaatca
    31381 aacggtgtga tttctgacat ggatttcttc ccaagcaaac gtaccactaa taataaaccg
    31441 atcaacccga acaccagttt cattgtaatt gaaaaaatca ttttttctcc ccctaactat
    31501 ctgttaatta aattatatat gctttctcac gaacacgcat ggaatataca aggaagaaat
    31561 gtatcctttt actaaaaaaa ggactgtgac actgtagtga cccccaaaag ttagactttt
    31621 ttggagtgga gaaatccact cctttttttt atgctctaaa atgatatttt tacacaaccg
    31681 ttacggttgt tttacg
//
LOCUS       DASJUF010000023        31184 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJUF010000023 DASJUF010000000
VERSION     DASJUF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.023"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(94..993)
                     /locus_tag="U4B62_000951"
     CDS             complement(94..993)
                     /locus_tag="U4B62_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141126.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(1158..1982)
                     /locus_tag="U4B62_000952"
     CDS             complement(1158..1982)
                     /locus_tag="U4B62_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2141127.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(1993..3288)
                     /locus_tag="U4B62_000953"
     CDS             complement(1993..3288)
                     /locus_tag="U4B62_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN2141128.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(3304..3783)
                     /locus_tag="U4B62_000954"
     CDS             complement(3304..3783)
                     /locus_tag="U4B62_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN2141129.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(3789..4817)
                     /locus_tag="U4B62_000955"
     CDS             complement(3789..4817)
                     /locus_tag="U4B62_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN2141130.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(4834..5940)
                     /locus_tag="U4B62_000956"
     CDS             complement(4834..5940)
                     /locus_tag="U4B62_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN2141131.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(6169..8766)
                     /gene="adhE"
                     /locus_tag="U4B62_000957"
                     /gene_synonym="adhC"
     CDS             complement(6169..8766)
                     /gene="adhE"
                     /locus_tag="U4B62_000957"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN2141132.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(9071..9451)
                     /gene="yajC"
                     /locus_tag="U4B62_000958"
     CDS             complement(9071..9451)
                     /gene="yajC"
                     /locus_tag="U4B62_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN2141133.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(9548..10693)
                     /gene="tgt"
                     /locus_tag="U4B62_000959"
     CDS             complement(9548..10693)
                     /gene="tgt"
                     /locus_tag="U4B62_000959"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN2141134.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            10911..11609
                     /locus_tag="U4B62_000960"
     CDS             10911..11609
                     /locus_tag="U4B62_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2141135.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(11777..14416)
                     /locus_tag="U4B62_000961"
     CDS             complement(11777..14416)
                     /locus_tag="U4B62_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2141136.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(15035..16066)
                     /gene="queA"
                     /locus_tag="U4B62_000962"
     CDS             complement(15035..16066)
                     /gene="queA"
                     /locus_tag="U4B62_000962"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN2141137.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            16259..17433
                     /locus_tag="U4B62_000963"
                     /pseudo
     CDS             16259..17433
                     /locus_tag="U4B62_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            17437..18078
                     /locus_tag="U4B62_000964"
     CDS             17437..18078
                     /locus_tag="U4B62_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141138.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            18075..18992
                     /locus_tag="U4B62_000965"
                     /pseudo
     CDS             18075..18992
                     /locus_tag="U4B62_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            18989..19660
                     /locus_tag="U4B62_000966"
     CDS             18989..19660
                     /locus_tag="U4B62_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141139.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(20201..20689)
                     /locus_tag="U4B62_000967"
     CDS             complement(20201..20689)
                     /locus_tag="U4B62_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN2141140.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(20890..21156)
                     /locus_tag="U4B62_000968"
     CDS             complement(20890..21156)
                     /locus_tag="U4B62_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2141141.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(21156..21419)
                     /locus_tag="U4B62_000969"
     CDS             complement(21156..21419)
                     /locus_tag="U4B62_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2141142.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            21895..22080
                     /locus_tag="U4B62_000970"
     CDS             21895..22080
                     /locus_tag="U4B62_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2141143.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(22222..24045)
                     /locus_tag="U4B62_000971"
     CDS             complement(22222..24045)
                     /locus_tag="U4B62_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN2141144.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(24643..27348)
                     /locus_tag="U4B62_000972"
     CDS             complement(24643..27348)
                     /locus_tag="U4B62_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN2141145.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(27654..28601)
                     /locus_tag="U4B62_000973"
     CDS             complement(27654..28601)
                     /locus_tag="U4B62_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN2141146.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            28791..29060
                     /gene="rpsN"
                     /locus_tag="U4B62_000974"
     CDS             28791..29060
                     /gene="rpsN"
                     /locus_tag="U4B62_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2141147.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(29236..29598)
                     /locus_tag="U4B62_000975"
     CDS             complement(29236..29598)
                     /locus_tag="U4B62_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2141148.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(29609..30730)
                     /locus_tag="U4B62_000976"
     CDS             complement(29609..30730)
                     /locus_tag="U4B62_000976"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN2141149.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(30746..31096)
                     /locus_tag="U4B62_000977"
     CDS             complement(30746..31096)
                     /locus_tag="U4B62_000977"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN2141150.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         8844 a         6733 c         5689 g         9918 t
ORIGIN      
        1 attttgaaat atatagataa aaataaaata accgcagttt ctgcttctat gtaaacaaca
       61 gcagctactg cggttatttt ttagtgatca ttctcattgt tcatcttcaa aatgttcata
      121 atgaaccaac tgtttctgtg tatcacgttt caaaaattct ttaggcgtta tgccgaactc
      181 tctcttgaat gctttcagaa aatgcggata agaagaaaaa ccacttttac tatatacagc
      241 actagagctg cggtattctc gcaacaagtg cttggcatag atcagctttt tcttcattac
      301 atactgatga aacgtaaatc cagtgtactt tttaaattct cgtgtgatat ggtatttgct
      361 gacaaaaaac tgcttttcca tttcctctaa agaaagcgga tgagataaat tttttgagat
      421 atattgtaac atttgttcca cacgctcatt aggaactaca gcattctgtt cttgcgcact
      481 ctcttctttt tgtacaactt gattgagaaa aatcaagtat tctaccagag cttgttcata
      541 ttttatgtct tcaccgtaaa tttttttatc aggaggatgg tcgataaatg ataatttttg
      601 cttcaatact tcctgttcta tttgcaatac acgactcctc ttctctccca ttggttgaaa
      661 acaagccgat agattagacc atggcgtgga acgactttct agaaaagaag gggtgacaaa
      721 gatatagaca cgctcgaata catctgaaga ttggcggacg atacgatgca aatcattgga
      781 atggatgagc agcacacttc ctgctttgat cgtaaattgt cgtccgttca aataaaaagt
      841 cgcttctccc tttaatgtag catggatctc atagaaatca tgatgatgat agatcgtttt
      901 attttggctg acgacatctt tgacatgata gatttcatac tgatcagaaa ccatcgtatt
      961 gacttgttca ccgctgattt tcttttggag catgtgccgc acctcctttt gttcacttat
     1021 gcacaatttt attataccac gttttttgta atcaaaaaga gctgctttta tatactctta
     1081 taaaaaagaa gagacgtggc atcttctagc cccgccccct ccattctaaa tattagttaa
     1141 ccaacgtctt ctttacatta ttcaagaaga cttcttccat aacgaatccc atcatctttt
     1201 gtttcttcaa gaaccacata caggtacgga cgattcttca tgatgatttc tttgattttt
     1261 gtgtactcga tcacaccgtc tcccaaattt accggcacga ttttttcctg ttcgacacga
     1321 aaatctttca aatgaaccgc acatatcttt tcaccaaaac gttcaaaagc ttcttctacc
     1381 aattggactt gttcttgata atcactactt gtgatcaaat tggtcggatc taaaatgatt
     1441 cctagatagt ccgaatctac tgcttggatc agctgctcta cccttgctaa agaatacacc
     1501 ggatgattca aacctggttc tatccccacc atcatttggt atttttctcc agcttttacc
     1561 aagcgacgga taacggacac agcttcagca aatgcttcat ctgtgaaatt ttcttccgta
     1621 tattggattt ccggcaaaac acatccagtt tccgaagcaa ccatcgacgc accaaaggaa
     1681 gaagcatagc gaagatactg ctcgaacttt gtcagcaact cttgtcgtat cgttaaatct
     1741 ggatgaatca tattgatata gcaacttaaa attcctacgg tcacttcttg cttttctaaa
     1801 actcgtttga cataatttcc catcccagaa ttgatctcat ttgcgccaga aggtatttca
     1861 ggaaacgaaa gacctaaggc gagctgcagt gtatgaatcc cttgttcttt tgttttttct
     1921 gcaagatctt ctatcgtttg gacttcggaa agatcatgtc ctctaattgt aatgttcaac
     1981 ataactattc tcctaactca gcatattggg tagccacatg acgatttgcg ggaagtaagt
     2041 aatcaaaaac aagacgacta agataacccc ataaaaagga agaagcggtt tcagcattgc
     2101 ctctgctttt acgcctccta cacttgcacc gacatatagt cctgtaccta ctggcggcgt
     2161 gatggagcct acacacagat tcatgattga aaacaacccg taatgtacag gatcaacccc
     2221 tacacttgtg acgattggta aaaatattgg tgtaaaaatc aagatagcag gcgccacatc
     2281 cataaacata cccaccagta ataacacaag attgactaat aacagaataa taaatttatt
     2341 atccgttacg cccataatca acgaactaat tgcctgagga ataccggtaa acgccatggc
     2401 gaaagacatc atggaagaag tagccaataa gaacatgatc gtccctgaca ttgctactga
     2461 ttgaaacaac atattaggta tatcttttag ttttaccgtt ttatagtaaa acatagcaat
     2521 caacagggag taaatgacag ctatcgctga agcttctact gctgtaaata tccccgtcaa
     2581 gataccaccg ataatgatga caatcaatag aaggctagga atcgcctctt ttattatttt
     2641 tccagcatga gccatctgtc ctttttcgac aacagggtat ttattctttt tagcaacgat
     2701 gaatgctact agcatgattc ctagcgccca caaagcgcct acgagataac ctcccataaa
     2761 caaagcagag atcgaagcac caccagcaac aagtgaatac atgataaaag cagtacttgg
     2821 aggaatgacc atacctgtcg gagctgacgc gatattgacc gctgtcgcaa acttccggtc
     2881 atatccttct tccacttgct gagggatcaa cactccgcca atcgcggtag aagcggcaat
     2941 ggccgagcta gagatggaac caaataaagc attccctaac acattcgtat gagccaaagc
     3001 ccccggcacg cgcccgacga acaacatcgc aaaatccact agtttcttag ctatccctcc
     3061 attattcatg ataatgcccg agagaacgaa aaacggaata gcgatcagcg aaaaactatt
     3121 gatacttccc accatttttt gagctgaact aaaagcagaa acatcaaacg gtataactaa
     3181 caataaagtc atcatcgatg agaatgcaat acttatagca atcggcatcc ctaatacaag
     3241 caacacaaca aatacaaaaa ataacactaa tccggcttct attgccattt tattcctcca
     3301 tttttatatt ttttcttgaa ttgctggctt gttgcttaat gtatcgatta gattgatcag
     3361 gctatagata atgatgagta cccccgatac aggtactgac agataaacat atcccatagg
     3421 aatacttaaa gaaggcgaga tctgccccat tgtcaatgtg acagcattcc atccgccata
     3481 caccatgatc atcgcagcaa ataccagaaa taaactttga acaacaactt ctaaaatttt
     3541 actttttttg ccagttaatt tttcacttaa aaccgttacg gctaaatgtg ctttttgtcc
     3601 cacaacatac gcggaagaca acatggcaaa ccagacaaga ccgaatcgaa caagctcttc
     3661 cgtgaccgta tttggatttc ctaaaaccgt tctggaaaat acttgccata aaacaacagc
     3721 gaccatccca acaagcaaaa cagctcccag gatctctaat accttgttta aaatcatttt
     3781 catcacagct acgctccttg ctcttggatc ttttgataaa ggtctttgta ggtaggatcg
     3841 tttttgaata tatcatgaag tggggcaact gcctctcgaa acggtgtatt atctacatca
     3901 ttgaattgga ctccatgttc ttcttgtgct aaagcttttt cttcttcaat tgcttttgca
     3961 aaagcttcaa tttcaaaaac cgttgattct ttcgctgctt cttcaatggc tttttcctgc
     4021 tcatcagtta gctgttcttt gatggcatca ttcatgatga tgatatctgg cacacgtgta
     4081 tgctcatcat aggaatagta tttagccaca ccaccatgtc cagctgtata caagacaaat
     4141 tcattgtttt ctgatccgtt gatcaaattg gattgaagag acgtgtacac ttcatcactt
     4201 cccataggta cgggcgaacc gcctaaaagc tctaccattt tgatagcggt ttcgctttgc
     4261 atcacacgga tcttctttcc cttcaaatct tccggcgtat ggatcggacc gtctaccata
     4321 taaaagctgc gctgaccaga atcataatag gtcaatccgg taaatcctaa atttttggtc
     4381 gattggtaga ttggctccat tatttcttca ttatccatca ccttaaagaa atgttcttca
     4441 tcattgaata gataaggaac tgaaaaaatc gaataatctt tagagaaact ttccaacgct
     4501 gaggcactta cttttgtgaa atctactgct cctgactgtg tcaattcgat cagttcacgt
     4561 tctccaccca gttgtccatc tgggaaatac tggatctgta cggaaccatc tgttttttct
     4621 tttactaaac gcccaaattc attcattgca tcaattactg gctggctgtt gcttgcataa
     4681 gcaaagcgca aggtaactat ctcatttaca tctgcatctg cgacagttgc cacactagaa
     4741 ttatttccgc agccgcttag acctagcaga cctaggctaa gcgaacttgc tgcaaacagc
     4801 agtgatgttt ttttcatatg ggtttccttt cttttaacaa taagagacga gcaattctgt
     4861 caaacaaagc accgttaacg actgtccata aggcattgct gtgattccga tattccgata
     4921 aaaatctaaa ctgtccccca ttcctgtccc gattgaaacg ttttgaactt ctcctttttc
     4981 atcgatttgt tcaagcaatc cttggatagc acgataagcg acttctttat attcttgtgg
     5041 taaataacgt ttatggaccg ctttcaaaat accgtaagca aatcctgcag ttgcagaaga
     5101 ttctacgtaa gaagaaggat catccaacaa cgtatgccat aaaccgcttt cagactgata
     5161 agtttttaat gcacgcactt gtgcttcaag tgtttcgatc aataatttgc gcaatgcatc
     5221 ttctttcgat aattctaaga tttcaatgat ctctggaata gcaatcgtca accagcagtt
     5281 tccacgagcc caaaatgctt cggcatagtt atgatttcct tcaaacgtcc agccgtgata
     5341 ccacaatcct gttcgtttat ccatcaagta tttgatatgg atcataaact ggtaacgagc
     5401 ttcttctaaa tattctgggc gattcaacag tttcccgatt tttgccaaag gaagcactgt
     5461 catcatcaat gtatcgtccc acaactgatt cttattttcc ggtccatatg tggcatgctg
     5521 taacccgtct tcatttgtcc gaggcatgtc attcatgacc cattctgccc attgttctaa
     5581 ataaggcaaa tagcgtgaat ctttcgtgtc ttcatacaag taagccatag tcaatagcgg
     5641 tgccattgta ttgacgtttt tgggcggagc gccttcttcc atacgatcct caaaccattc
     5701 attgaccact tgataagcgc gaggattttt tgtcatctgg taatttttat aaataccata
     5761 tagaccgaca ccttgtggcc aattccacac tgtccagctt ttgtcatcta ctttcagtcc
     5821 atcaaagtcc aacaaaaatt caccattatc gtctttgatc gttgtcaggt tttcaacgag
     5881 caggtcaatt ttttctttca ctaattcttt aggtacaaaa atctcttctt taataatcat
     5941 ctttacgcca cctttttaaa cgctttcatt ttatatcctt aatatagcag taataccgtc
     6001 cactataaat atcgcttttt gaacaaaaac tgtctaaaat tgacctaaat gttaaataac
     6061 aaaagtaact ttcagttact tggaaattaa aaaaagaact tggagaaaca acgttttcca
     6121 agttcttttt catctatttt cttatctttt ttctacgctt tcttttgctt atccgttgta
     6181 agcatcaatg atgatctgtt tcaattcaga gatcaatggt tctttcggat tagctgtcgt
     6241 acattggtct tcgtaagcta attcagccat acggtctacg gttgtatcta gtgtttcttg
     6301 tgtgactcct tgagctttta gactcatgtt gatgccgaca gattttccta attcataaac
     6361 agccgttgct aaagcttcga ctaattcctc tgttgtttcg cctttcagac ctaagaattt
     6421 tgcgatatct gcgtaatctg tatctgcacg gaaatagtca tatttaggga acatcgcatg
     6481 tttttgtgga tcttttgcat tatagcggat aatatgcggt aaaagaatcg catttgtccg
     6541 tccatgaggg atcccgtatt ccccgccgat tttatgtgcg actgagtgac aaatacctag
     6601 gaatgcgtta gcaaacgcca taccagccat tgtagaagcg ttgtgcattt tttcacgtga
     6661 ttccatatca ggtgttttta ctgatttttc taaatagtca aagactaact tgattgcttg
     6721 taggctcaat ccacgtgtat aatctgaagc catgactgaa acataggatt cgattgcatg
     6781 tgttaacaca tccatcccgg tatctgctgt gacagatgct ggaactgaca tgacgaattg
     6841 aggatcgacg attgcgacat ctggcgtcaa tgcataatct gctaacggat atttcacatg
     6901 tgtttcactg tcagtgatga ccgcaaatgg cgttacttca gaaccagtcc ctgatgttgt
     6961 cgggatacat acgaattgtg ttttttctgg tttgtcgatc ttatatgtcc gtttacggat
     7021 atccaagaat ttttgttttg caccaaagaa agatgtgtct gggtgttcat agaacatcca
     7081 cattccctta gctgcgtcca ttgcagaacc gcctccaaga gcgattactg tatctggctt
     7141 gaagtctgcc atcagtttcg ttcctgcgta tacggtattt gtcgatgggt tcggttcaac
     7201 atctgagaag acttcgatct tcacatcatt tttacgtcgt gccaatactt cacgaactgt
     7261 atcgcagtaa ccgaattgga ccataccagg gtcacaaacg atcatgacac gttcaacgtt
     7321 ttccattttc tctaagtaca atagagagtt cttttcaaag aagatttttg gcggtaattt
     7381 gaaccattgc atgttatttc tccgtttcgc tacagttttg acattgatta agtttacagc
     7441 agagacgtta cgagaaacag agttcttacc ataagaaccg catcccaaag tcaatgacgg
     7501 aatcatttcg ttatagatat caccgatgcc gccttctgca gatggtgtat tcactaaaat
     7561 acgacaagct ttcattcgaa gaccgaattt gacatgcaac tcttcgtttt ctgaatggat
     7621 aactgctgta tgtcctaatc ctccaagatt caacatacct tcacacaaat caaacgcatg
     7681 gtccgtgttg ttagctttga tcatggcaag aactggagaa agtttttctc ttgatagcgg
     7741 ataatctggg cctacgcctt ccaattcagc aatcagcatt ttcgttcctt ttggtacact
     7801 gattcctgcc aattttgcga tttccatcgc agaatgtcca acgattgatg gattgaccgc
     7861 atattttcct tcgttcatca ctgcatcttc taatttttgc aattcatctg gttttacgat
     7921 ataaacttgg tgtgcttgga attctgcttt tactgctgca tagatttctt tatccacgat
     7981 cacagcttgt tcagaagcac aaatcattcc attatcaaat gttttcgaaa cgatcaaatc
     8041 gttcactgca cgtttaattt ttgctgtttt ttcaatataa gctggtacgt tacctggacc
     8101 tacacctaaa gctggttttc ctgtagagta agcagacttg accattccag ctccacctgt
     8161 cgctaagacg atcgcgatac ctggatggtt cattagcata gaagtcgctt cgatcgaagg
     8221 atgttcgatc cattggatac aattttcagg agctccggca gcgatagcag catcacgtac
     8281 cactcgtgct gcttctgcag aagatttttg ggcacttgga tggaaagcaa aaacgattgg
     8341 atttcttgtt ttcaacgcga tcattgattt gaaaatcgtt gtggaagtcg gattcgttgt
     8401 tggagttact ccgcaaacca caccgactgg ttcagcaatt tcaatcaacc ctttttggac
     8461 atcttcattg atcacaccaa ctgttttatc atgtttgata ttatgccaaa tatattcgga
     8521 cgcatacata tttttgattg ctttatcttc gtaaacaccg cggcctgttt cctctacagc
     8581 catcttcgct aatggcatat gttgatccaa agctgccatt gccatttcat gcacgatatg
     8641 gtctactttt tcttgatcaa agctttccat ttctttcaat gctgcatttg ctttttctgc
     8701 caaatcatca atcattgctt gtacgtcaat tgtcttcgtt ttttcttttt ctattacttt
     8761 agccatcatt atcctcctaa tgggtcatgg ttatttgtta aataattcac atgtatatca
     8821 tacaatattt atttttctat gtaaacactt ttttgtgatt tttttcacag ttatttttaa
     8881 atataaaatt gctttattta caggtgtttg tatagaaaaa tatataaagt tatttattat
     8941 tatttatatg acacacccga ttattcaata ttgagcaata tatcccatgc tcttatctct
     9001 ttcttttttg aataaaaaat gaggtaggac atctttctgc cctacctcgt ctttaaataa
     9061 gctttgatac ttatttttct tccttctctt ctgtctcttc cagcgtttct gttgtttcag
     9121 tttttacttc aactgtctca gctggttctt ctgttgtttc aacagtagtc gttgcatcat
     9181 ttgttacgac agtccctggt ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga
     9241 tcccttcgca gtctaaaaca actgtccgtt tgtcattgtc gatttctgaa acaactccat
     9301 gtaatccgcc gatcgttacg actgaatcac ctggtttcat gctatctaac aagttttgac
     9361 gttcttgctg ttgtttcttt tgcgaacgtg acataaaaaa ccacattgct aatagcgcga
     9421 taaacatcac aagcattggt aatgttccca tttttttcac ctcactaatt ctgtatctac
     9481 aaaaaacact ttagcagaaa aatgctgata acactagttt tttacttaat ttttaaaaga
     9541 ttttccttta gaagcttttt gcgttttctt tattgaaccc gtattcttca aaaaaggctt
     9601 ctctaaattc aagcaaatta tcatccatga ttgcttgacg tacttgtttc attagattca
     9661 acaagaagta taaattgtgg taagaagtca aacgaatccc aaatgtttca tcacatttga
     9721 tcaaatggcg gatataggca cgtgtatagt ttttacatgt ataacaatca catttctcat
     9781 caattggacg gaaatcatgg gcatatttcg catttttcac tactagacgg ccttgagaag
     9841 tcatacatgt gccatttcga gcgattcgag taggcaagac acagtcaaac atgtctactc
     9901 cacggataac accatcgatc aatgaatcag cagcaccgac acccatcaag taacgtggtt
     9961 tgttttcggg gatcaacggt gtagtgaatt ctaatacacg attcatttct gatttaggtt
    10021 ccccgacaga taacccgccg attgagtaac cagggaaatc catcccgact agatctttcg
    10081 cgctttgacg acgaagatct tcaaaccctg ctccttggat gattccaaat aatccttgac
    10141 ggtctggatt agcatgagct ttcaatcctc gttctgccca tcgagacgta cgttctactg
    10201 attttttcac gtagtcgtaa ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga
    10261 tatcagagcc caatttattt tggatgttga ttgctttttc tggtgataaa aacatacgcg
    10321 aaccgttcaa gtgatttttg aaatgcacgc cttcttcttc gatattccgc atatcactta
    10381 atgaaaaaac ttgaaagccg cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt
    10441 tatgcagtcc gccagcttct tctactaagt cttcaccagg gcgtagccat aaatgatatg
    10501 tattacttaa aataacgcca gcacccattt ctttcaactc ttctggggac attgttttta
    10561 ctgtagctaa tgttcctact ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga
    10621 tcaattcccc taaacgcgcg ccagtatgtt tttctttctt gattaaacga tagcgaatgg
    10681 caggttgcgt cattcatttc cctactttct atataaaata tcttgacaac tcccaccatc
    10741 ataatcgttt caagtgcttt ttgcaagttc cctcggcttc cttctacttt atttaatatt
    10801 ctttttttta tccttttttt atgaataaca aaaaaaagaa aaacattatg acagaaatat
    10861 tttttgatac atggtatatt ggtgttatta aagaaaagga gggaaaaatc atgcaatctt
    10921 ctgcagaatt aaaacgccaa gcaaaagaga gtctaaaagg acgttgggga caagctgttt
    10981 tactaaatct aatccctaca ttgattggta ttgctactat tcttcttatt atgattccta
    11041 ttatcttgtt tgccaccacc atatatgatg gatcagcgag cgtctcagaa gcgacaactt
    11101 caggaggcgg tagttcagga ggcggtggcg gaattatttc cagcatcatt agcgctttgt
    11161 tcatgagcgg tatttcttgg acttatcttg atattttacg tggacaaaaa gatcagatcg
    11221 aaccattcaa agatgcattt cgtggattca aaggtttgtt tatcggcgga gtgatcagct
    11281 tagctgtgct gatctcgatc tttacaacat tatggggttt gctattagtt attcctggaa
    11341 tcattaaaag ctattcttat tctcaaagct attttattta ctacgatatc gtccaagaaa
    11401 caggacaaac acctaaagta ctggatacga tcactgcaag tcgaaaattg atggatggtt
    11461 ataaaggaca acttttctgg ttagatcttt catttatcgg ctggcatatc ttagcattag
    11521 caactgctgg tattggttat ctatggctca gcccatacat ttctgcaaca aaagcagcat
    11581 tttatgaaca attgccaaaa gatatttaaa agagagtgtg acttgtgacc gagggggtgg
    11641 caggagaaca ccgtttatcg agaggcagta aaagaaacat tcagcccatt agcaaaaagg
    11701 aggtgtgaca acattcatgt cacacctcct ttttgtatag ttccagtaaa aagaaaatca
    11761 acttagaaac agttaatcat tcctcttctt ctgcttccgg taatcgatgg aacttgatca
    11821 gttcattcac cgcgattggt acgatagata aaatccctac caagaaccag tgattcaaac
    11881 tgatgtgtac taggccgaat agttcttgcg taaatggcag caaagcaaca gccgttgtaa
    11941 tagctaatgc cagtaacgcc atttctacca aagctttatt ggtcgctaaa ttgactttga
    12001 agatcgattg gctgctgcgt acattgaaca catgcagaat cgatgaataa gctaagatca
    12061 agaatgcaac agtttgaccg acatgctgac ttgcgtctac ccatgggctg atagtatcga
    12121 cataactacc taaataataa ccaaacaagg ttacgatggt gaagacccct gcatttatgg
    12181 caattttttg caataaacct cgagcaaaga tcccttcatt tttcggaatg ggtggctgat
    12241 ccatgatgcc gtgttccgct ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa
    12301 ctacattgac aaacagtagt tgcacagctg taaatggtgc tccccatcct aacagcatcg
    12361 caatcaaaac aataaagatc tccgaaatat tacagcttag caaaaagttg atagctttac
    12421 gaatattctg ataaaccgaa cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt
    12481 catccgtcaa aatcatatct gcggcacttt gcgccacatc tgttcctgtg atccccatgg
    12541 cacacccaac gttacttgct ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg
    12601 ctacgactgc acccgtacgt tgccaagatt tgacgatccg gatcttgtct tctggcgtta
    12661 cccgagcata gacagagaga gctttgacac gcgcatctaa ttcttcgtct gataattgat
    12721 gcagttctga tcctgaaagt gcttctgacg gatcattcaa gatacctaat tctttcgcta
    12781 tggcactggc agtcaccaca tgatctccag tgatcatgac tgttttgatg ccagcctttt
    12841 tcgctcgctt gattgctcct ttactttctg gacgtggtgg atcgatcatt ccgatcagac
    12901 caatcaaacg taagtctttt tccagtgctt cagaagaaat atcttgcggt tcttcgtcat
    12961 aagtagcata accaacagca atcactctca gtgcccgttt accaaagcga tcattaacga
    13021 tcgccgcttg ttctacatcg cctgaactaa agcgcggagc aagaacatcg aatgctcctt
    13081 tggtgattga gatgtatttt tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag
    13141 aatcaaaagg aagctcgttt actcgtggat atttttcttc taattctgcc tttgtatgat
    13201 aattctcttc aacagccctt acgatggcta cttctgttgg attacctttg ataacaaggt
    13261 cgtcatcttc ttgctcgacc gtcacatctg tacatagtgc agccattttc aatatttcca
    13321 tcgcgctgtt cgtcattgaa tcttcgatat tcgttacttc atcttctttg ctccataccc
    13381 ggcggacgcg catttcattt tgtgtcagcg ttcctgtttt gtctgagcag ataacattag
    13441 cggttcctag cgtttcaact gctggcaact gtcggatgat cgcatgtttc ctcgccatct
    13501 tctgcacacc gtatgcaagt gtcaacgtaa caataacagt caaggtttcc ggtacagctg
    13561 caacagccaa ggaaatcgaa gtcatgaaca tctcaaggag tggttctcct tgcagttcac
    13621 caatcacaaa tactaaagcc gcagccgcta aagcgatcat actgattcgt ttgcctagct
    13681 gattcaatcg gacctgtaaa ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga
    13741 tcttacccat ttctgtagcc atgccaatgg cagtgacgat cgctttccca cgacctgcag
    13801 caaccgtaca tcctttaaat accatattaa gttggtcacc taatgactca tcttcttcag
    13861 caataaatgc tgcatctttt tcaactgctt cactttctcc agtcaacgcc gattcttcta
    13921 cacgtaattg tgatgcttcg atcaaacggg catcggcaga aatagcatca ccggactcta
    13981 aaacaagaat atcacccact accagttgct ctgactcaac agtggtcact ttgccgtctc
    14041 gtatgacagt tgccgtttgt ttattcatat cttgcaagga ttccagtgct ttttctgcgt
    14101 ttccttcttg gacaatcgcc aataccgagt tgatcacaac gatagcgatg atcagcagtc
    14161 cttcaaataa atccccatgt tctgtagcaa atgcggtata aaaggaaatc accgctgcta
    14221 caagaaggat gatcgttgta aaatcggaca agcttcgaaa gaattttttg atcatcgatt
    14281 ctttcggcgc ctcatcgaat ttattcaatc cctgcttttt tcgccgttca tccacttctt
    14341 gtgaagacaa cccagtatct ggatctgctt ctgttacctt ctttacttct tctattgtct
    14401 gcttgtattc ttccatccta ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt
    14461 ttactataac atcctttttt tatttacttc aaataaaggg cttatcgatt atttgttaag
    14521 aaagcgtaaa atttaaagtg cttatatttg caaacgctac caaaggatgc tattatatag
    14581 atgtaaggaa ggaggatacc atcaaataga cagaagaatt tctcgataga acagtgtaag
    14641 caaacaaagt tgttagaagt acaggtttca cccagacatg cgtgtagagt acaaacctat
    14701 aaatagtgaa tgcgtatgga tgtttggatc acgaagtatc cgaaacaaga agtaatgcga
    14761 tacgtaacaa ttgaataacg taaacgtctg aaaattgaat aggaacaaga gatgtttcaa
    14821 atgttgaaga tgtaacgaaa acaaaatttt tgtttaacat gaagaaattc aacaccgtgc
    14881 tttgcatgta tgatggaaag ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca
    14941 agaaagccga gcttatcgag agctcggctt tttctttgtc ataaaatctt tgtaaagctt
    15001 ttttgttatt caaaactagc ggcttttcat aaccttattt cacgaacatc gcatctccaa
    15061 aactaaagaa acgatagcgc tcttcaatcg catgctggta agcagataaa gtcaattctc
    15121 ttcctgcaaa ggcactaact aacatgacta aagtcgattt tggcaaatgg aaattagttg
    15181 agaaagcttc taccactttg aattcatagc caggcgtaat gaaaatatca gtccatccgc
    15241 tgtctgcttt gatttctcca ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag
    15301 ttgttccgac agccacgatt ttcccgccat ttttacgtac ttggttcaat cgctcagccg
    15361 cttcttccgt caaacggtag aattcactat gcatatggtg ttcttcaatt ttatctacgc
    15421 tgacaggtcg gaatgtacct aaccctacat gtagtgtcag atagactaat tcaacaccct
    15481 ttgcttcgat ctcatcaagt aattctttcg taaaatgcaa tccagctgtt ggcgctgcag
    15541 cagaaccatt ttctttcgca tagacagttt gatagcgatc tgggtcttcc agacgttctt
    15601 tgatatatgg aggcaatggc atttctccca atgattcaag aatctctaga aaaattcctt
    15661 catactcaaa gtcaatgatc cgtccgccgt gttccaattc ttctttcact actgcttgca
    15721 atctgccatc accaaaagag attcttgtcc caactttggc acgtttcgca ggtttgatca
    15781 atgtctccca cgtatctcct tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag
    15841 tatctggttt ttctccatat aaacgagcag gcagaactcg tgtattattc ataactagcg
    15901 catcaccagg ctgtaactct tcaatgattt catggaaatg tttatcttcc atctctccag
    15961 ttttccgatc caaaacaagt aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga
    16021 ttaattcttc cggcaagtca aaatcaaaat cttctgttgt taacatatcg tcactcactt
    16081 tctatcatgt gcttcatcat tctatcatgt ttttcgttct gttggcaaaa tcagcacttt
    16141 ttcacttcta ttttttgttt tcctaaataa aaaaactacc ctatttgtat tttttcattg
    16201 ttttaggcat actagtaata gatagttctt cattgaaaat agaaaaagag gtggcaccat
    16261 gattgaattt caaaatgtgt caaaaattta taaaggtgga aaagtcgctg tagaggacgt
    16321 caatctttca tttgaaaagg gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa
    16381 aacgacgacg atgcggatga tcaaccgaat gactgatcca acacaaggga aaattttgat
    16441 tgatggaaaa tccatccaag aaattgatcc agtggaattg cgacgtcaga tcggctatgt
    16501 tatccaaagt attggattga tgcctcacat gacgatccgt gaaaatatca ctcttgtcca
    16561 aaaattgttg aaagtcagtc aagaagaacg gaataaaaca gctgaaaaaa tgattgatct
    16621 tgtggaattg ccccgtgaga tgttagatcg ctacccacat gaattatctg gtggtcagca
    16681 gcagcgtatt ggagtcgttc gtgcccttgc agcgaatcaa gatatcatct tgatggacga
    16741 gccattcggt gcgcttgatc caatcactcg tgattccttg caagaccttg tcaaagattt
    16801 acaagagcgt ttgggaaaaa cgatcgtatt tgtaacccat gacatggatg aagcgattaa
    16861 actagcaagc cgtatcgcca tcatgagtga aggccgtgtg atcaatttga tacaccagaa
    16921 aatattttac gtcatcctgc aaacgatttc gtagaagagt tgattggaga agatcgccta
    16981 ctacaagcca agccaaatgc gacaagagtc ggcgaagtta tgctaaatac agcaatcacg
    17041 attacaccgg aaaaatcatt acaagaagca atcaaactga tgagggaaaa acgtgtagat
    17101 acgttgttag ttaccgataa ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg
    17161 aataaaaaac gaggaaaagt atccagtgtc ggcgacattt tgaataaaca tgttttttac
    17221 gtaaaagaat ccgcctatct tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa
    17281 tacgtgccgg tcgttgatga acagaacaaa gtcgtaggta tcctgactcg tgcatcatta
    17341 gtggatatcg tctacgatgt gatttgggga gaagatcccg caactactga tgttgcagag
    17401 tcagctactc ctgaagaatc taaggaggtc tgacctatgc agcagttttt tcatcagcac
    17461 ggttcagaaa ttctctcaaa aggttttgaa catctttata tttcaggact tgctttgatc
    17521 attggtatca tcattgctgt accgcttggt gtcttactga cacgttttcc aaaagctgcc
    17581 acgattgtga ttggcttaac cagtgccttg cagacaatcc cttctcttgc tttattggca
    17641 ttgatgatcc cgctatttgg cgtaggtaaa ttacctgcga ttatcgcact ctttatttat
    17701 tcattattgc cgattttacg taatacgtac attgggatga aaaatgtcga ttggaattat
    17761 cgggatgtgg caaaaggcat ggggatgact gagttccaat ccattttttc cgttgaattg
    17821 cccattgcta tgccaacgat catggctggt attcgtttag ctgccgttta cgtgattgca
    17881 tgggctacac ttgcctctta tatcggcgct ggtggtttag gcgatttgat ttttagtgga
    17941 ctgaataatt accaaccaga tctcattttg gcaggtacga tccctgtaac aattttagca
    18001 ctattggctg attggcttct aggtctcttg gaacaccggc tgacaccgat cgcacttaga
    18061 gaggagaatg aagaatgaaa cgtatactaa aaatgaccat ggttcttttc tctcttctta
    18121 tacttgcgag ttgttccttc cctggtcttg ccagcaatac agatgaagac accatttcga
    18181 tcacaggtgg aatcacttca gaagcacaga tcttagcaag tctagttgcc ggaatggttt
    18241 aacattatac agataaaaat accgcaatca tcaacaatct ggctactaca actatcaacc
    18301 atcaagcgat gatgaacgga gatgcttcaa tttcagcggc tcgctacaca ggaacagatt
    18361 taacgactac attgaacctt cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga
    18421 aagatgaatt tgagaaaaga tacggtcaaa cgtggttccc gtcctatagt tttgaaaata
    18481 cgtatgtctt tcttgttcga aaagacaccg cacaaaaata ccatctatct aaagtcagcg
    18541 atctgaaaaa tgtagcggat gaattagtag ctggtgtaga tacttcttgg atcaatcgta
    18601 aaggagacgg ttatgacggc ttccaagaaa cttatggctt ctccttcaat tcgatattac
    18661 ctatgcagat tggacttgtt tatgacgcag tcgaagcagg aaaaatggat attgttcttg
    18721 gctactctac ggatggacgg atcgccagtt atgatctagt catgttggaa gatgatcgtc
    18781 aattcttccc accatatgat gctgctccag ttgtagacaa caagatatta gctgatacgc
    18841 ctcatttaaa agaagcctta aataagttag gtcataccat cagtacggaa aaaatgcagg
    18901 aattgaacta cgaagcagat aataatctag tggaaccatc agtagtagca caacgtttct
    18961 tacaagaaaa ccactatttt gaagggaagt gaagcaattg gataaaatga atgtctttca
    19021 acaatttttc tattatttcc aggaaaatgg ctcctatatc tttgcacaat ttatccgtca
    19081 cttcctgatt tctatctatg gtgtcctctt tgcagcagtt gtcgggattc cggttgggat
    19141 catgatttct aggagacgta aattagcaaa ctgggtgatt cgtttggcga atatcatcca
    19201 gacgatccct tctctagcca tgatctccat tttaatcatt ggattaggcc tgggagtcaa
    19261 tgtcgtgatt gttactgtat ttttgtattc cctcttaccg atcatcaaga atacgtatac
    19321 cgggatgatc caagtagaca aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc
    19381 tagacaacga ctattcatgg tagagttacc actctctgtt tctgttatca tggcaggcat
    19441 aagaaacgcg ttagttgttg ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg
    19501 tggtcttgga gatatcatta tccgaggaac caatgcaaca gagggcacat cgattatttt
    19561 agcaggtgca ttgccaactg cattaatggc gatcatcacg gactggcttt taggtatttt
    19621 ggaaagacgg ctagatccag ccagccgtac atcacgctga tgttattgta aaaaacaaaa
    19681 gcttctgcga ccatttgttg gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat
    19741 aatccactct gattcttccc tccttatggg caaagagttg cttttcttag ctatttatcc
    19801 attccgtata ctccaacttc atactgccaa cgggttcata tctgcatcca ccgactaagt
    19861 ttttttctta tccgctaagg cattttgcca atccattttc ccatcactcg tcatggaatt
    19921 cataccacat gatctctaaa tactcgtctg cttgttcttt gaattgggca tcaattctta
    19981 catcctttgg cgtggctttc atttaagcct tttccatcag tcctcgattg ttcgatgcct
    20041 tcagctgcat catcaaaact ggtgactgcc gtatcggtaa cttcgatcat cttttataca
    20101 atcgggaact tgccatcctc atccttggct aaaaacatat ctagccattc cagcttctcg
    20161 ccatgggagg tttgcggttc ctaaaaccgg tacacgattc ttatggactg catccgtcgc
    20221 aactagactt ggcgatacga tgatccttca ccagaagagc acccccgtaa accaattgat
    20281 cgatatattg ccaataagaa aagacattgg cgtaaaagat atcgatccct cgtgatgcat
    20341 tgccgttgat actgctattc atggccgaaa gcgccacgac tttaacttct tccgtttgag
    20401 tgacattcat gttcggcaat gtcgctttgg atacccgctc tgctaaagga acgacactgg
    20461 tattgtattt cgtatctgta tcttttccaa aagaccatgc caacgaatcc tctgggaacc
    20521 aataagacga ctccggttga ttgtccattg cttttttcaa catacttttg gctgtttctc
    20581 gataaacgac atttccaatt gtcttcgcct cactttggcg ataatcgatg gtcgttgcca
    20641 ctgccaacac taacaacgat gctccaattt tactgatttt ccttgtcaca cttcttcctc
    20701 cttgttttac ataaacccgt caacattcca ttcctaaact caaacaatga cccttcaaag
    20761 gtggagatat aggaacaaat taaagaaatc atgcaataaa tccaagaagg ttggttttag
    20821 aacaaaaaaa gagtctcctt tttggggaga ctctttgtta actatctaaa aaggttggct
    20881 cttctcactt cactcataat gtgtccaggc agaataaatt tctacaactt tattttcctc
    20941 atcaactttg taaaccactc gatgctgtct attcagcctg cgtgagtatc tgccttcatg
    21001 tgtaggtctc agcttttcaa aagactgcgt gggcatatat ggatcttcct tcaatgtctg
    21061 aatcacttct tcaaattgtt tctttaaatt cgtttgcttg atttttctta aatccacttt
    21121 cgcagagttt ttgattgcga ctgtatagtt actcatcaaa gagcttccca atcaatatca
    21181 tcgatattcg taaagctact gtcgtctttt tcgcggtctc gtactttatc taaagtacct
    21241 gtttgctcaa gaaaaagggt ttcttgaatc gctctccagt cttccagccc aatcaataca
    21301 gcattgtttc cactacgatc actaataatc tcgatttcag tatgattttc atttacctct
    21361 ttcaataact gataaaaatt tttacgagca gtgctaggat tcatcgttgt attagccatt
    21421 gtctctcctc ctttttctga tataattata gcgtacttga ttaagtacgt caaaaaaata
    21481 caataaagca cttaaattca gcaaactttt cctctttttt acatagagaa aaagacccct
    21541 ttttagggag gatctttgtc cacgctcact tcttactcct ttacgactag cggtagccaa
    21601 acgtaaagta attggtaaca acgtatcatc tttagcaaag gtcgaatcca aagcctgtga
    21661 gtctatgcaa caacaccaat acacgaaggt tcctcgttgc agtgtgactt gattgatatc
    21721 ttgaataacc tgaggatacg cacgaaagat ttgtaacggt tattcaaaac tgattttggc
    21781 cgtcaagtct tctactgcct caaaaatgat tgcctctttc gtctcctgtc attcatgttc
    21841 actatcgatt tgatacttgc ttagaccgtt gggtttgtga atcaccaatt aaaaatggca
    21901 ctgagttatg gtaacaaaaa atggtcactt gacgaggctg agattcctac ttatgaagac
    21961 acatggcact taagagtcga ggtaaaggaa gaagctgaaa atataggagg ctgtacatta
    22021 tttggcaaag gattcggaaa ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa
    22081 ataggaagaa taataacatt tcatacgctg ttcccatctt taatggctta tagagggcac
    22141 ctttagctga gaataagaca aaacagaccc tctagcttca attaagagct agagggtctg
    22201 tttttgattt taaaacttgg tttatttctt caaatggtag ctataggtgc tgacaacgat
    22261 attttcacgc caagacaatc gcaatcttaa ccgcaaactt gtttggttat ggagaatatt
    22321 ctgccagcgt ctgtttggta caaattgagg aatcaacaca gtagttgtat agttttgttt
    22381 cgctgcattt ttacttacga tatctacata acggataatt ggattttcaa tcgagcgata
    22441 agaagagtgg acgattgaaa aacggacatc tgggaaatgc tttttaaatt ctgcttcgat
    22501 ttctttttct ttttcgacat cttcgtctag cgaaacatgc atcgcaacga cataatcacc
    22561 gatcgaacgt gcgtaattca acgcaccgat atttacccgt gtcacattcc cgactaatac
    22621 aagaacggta ttcccgtcat aatcatgtag ttttgcatca ttttctaaac gaagctgttc
    22681 tgctactttt ttataatgat catggatctt ataaaaaatg aacataacga ttggcataat
    22741 gatgaagaaa ggccaaatgt cccctaaccg atagacaaac aaaatcgcaa tgattgcata
    22801 tgagatgaac gcacccgtaa tattggcaat cgatttgctt aaccatcttt ttccttcttt
    22861 tttccatttg acgaccatcc ctgtttgaga taaagcaaat ggaatgaaca cgccgataga
    22921 gtataacgga atcaagcgtt ctgttgagcc atggaaaata aacagcaata cgatcgaacc
    22981 tagcgctagt gtgataatcc cattggaata gcctagtcga tccccacgat cttgatacat
    23041 gtgaggcatg aatttatctt ttgccagatt gtatgcaaga actggaaaag cagaaaaacc
    23101 ggtgttagca gcaactgcta aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat
    23161 accatgtcca aagacagctt taccaatttg agacaaaaca gtgacttctt tttcaggaac
    23221 gatcccatac caatagttga taaatgtgat ccccacgaag aagaaaccta agatcaatgc
    23281 catcatcgtc aatgtagcag cggcattttt tgctcttggc ttcttaaaga atggtacagc
    23341 attactaatc gcctccaccc cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat
    23401 caaagcgata gaaacacccg gcacagccgc tcctggaagt gcagtagctt gcaatggcac
    23461 agcacctgtc acgatattat ataaaccaac aacgatcagt aacgagatca caaggataaa
    23521 tgtgtacacc ggaaataata ggaacgatgc agattccctc agcccacgaa gattcagcat
    23581 catcagtagc aaaacgattg tcacagaaat agcgacttga tgaccgtaga gtgctggcac
    23641 cgctgatgtg atcgcttcag cacctgctga gacagatact gctactgtca acatgtagtc
    23701 gatcaataaa gagccaccgg aaatcaagcc agcattcttt ccaagattct cactgctgac
    23761 gacatatgct cccccgccat gaggataagc atggatgatt tgacgatatg atagcgttaa
    23821 tgaaatcaat aaaataataa caaaagcggc aatgggtagc gaataccaga tggctgcagc
    23881 agacaaagcg actaaaacga caacgatttg ttccgtacca tatgcaatag atgataacgc
    23941 atcagacgac aataatgcca aagcagcaaa tcgggttaat ttgtgctcat cattttcagc
    24001 agattttaaa ggcttaccta ctaacaacct ttttaagtaa tccaatttct aaactccttt
    24061 acacacaatt ttacttcaag ttttatagtc gaaaatttat agtaaacaag atgttatagt
    24121 acgctcactg accttaaaag tcaataaact ttaagaaaat aattggaaaa aaacagaaaa
    24181 atatcaagcg ctttttcaaa cgctttaata cagcaaaaat ctaacatttt tttatgggct
    24241 ttttctttga attctataat aaaaggatag aacttgactt agaaaatcaa gttctatcct
    24301 ttttgatatc ttaacttttg gaagagagat gtaaggctgc tttctaccca tcattcttac
    24361 tactcgaagc atagcgcagt tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat
    24421 ctgcggtaat aagcccaacc aaattaaaaa cataagaagc tgtttttatt tggtcgttct
    24481 tattacttga tgcagagcag cttttttaca acctcttggt acacggtgtt caatcagctg
    24541 accctcggta ttagctcaaa aaaacggcaa aatatgagga actatttttc catttttttc
    24601 tctaatactc aggtcaaaac actgatttca caacctcttt ttttaatgaa aaatatggct
    24661 ctcatgcgcc gcttctttga atttcgtcat tttttcttcg atgaacggat agattgccgt
    24721 tcctacgatt ccaaagacaa caaacaaacc gatcatgatc cagattgctt tcgtagcatt
    24781 tggccaataa attcctcctg cagattcccg taacaagttt accgcatgag taaatggcaa
    24841 gaacggattg atcatttgga agaatttccc tgagacttgg atcggatagt tcccgcctcc
    24901 gccagagatc gacagtacta agatgataat agcgatccct ttaccgacat tatcgaataa
    24961 tgcagccaag acatagacga tcatcatgaa cgctaaggcg atcaagacag caaatgacac
    25021 gctgtagacc ggattttgga catctactcc tagtaagaaa atattcccta atgtaacgat
    25081 caagctttgc gcaatcgcca ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg
    25141 tttcgtaaaa ttgtttcggt ctttctttcc taaatagtat tctgtcgtag caacacttga
    25201 aagaagaaca gcgccgaccc acagacatag tgcagtataa aacggggtgc tagctgaacc
    25261 gttatttgcg attggataaa gcgcattggt ttgtagctca acaggttgtg taaagaagtc
    25321 actttctttt gttgcgtcta gtttcaataa tttgatgact tcgcctagat cgacttcttg
    25381 ttcgcctttt cggatcgcgt ctgctgcttt ttggatacca gctttgatat ttggccagtc
    25441 attgttgatc aagtccgcag cagcgttcac agctgtttcg acttctggca ttttttcatt
    25501 tactgttgtc aatgtaccgg tgatttcatt ttttagtgct ggccaatcat tttggataaa
    25561 atcagcggcc aatcctaatt tttgctgaat gatcggtaat tcattgttgt ataggtctgc
    25621 cccttcattg atgccattga cgatggtatc catatttcca ttcagcattg tattggcatc
    25681 atggatttct tgtccaattg caggcatttc tttttgatat ttttctaaaa ttgccactgc
    25741 attcgttaca gttgctttcg ttgagctgag aagcgaatca aagtcgatct ggtccgcttt
    25801 gctcagtgcg ccttgtgcag tcccaatcgt atcgatgact tggtctagta gagaagtgac
    25861 agtggaactg atttgacctg tattgatacc agcaatgatg ccattgatat tattaacaac
    25921 ggcatcaatt tgatctagca attcatcaat agccttcgtg tcttcatttt gaacagctgt
    25981 gttcaaagca gataggcggt tttgcagatt gcttagcaag ggttttaatt gatttagctg
    26041 atcaataatt ggctgtaatt tcgtatttcc agcacttgtt tgcaatttag tcaggaactc
    26101 gaccagttta tcgattgcat tttgttgttg agatattccg tcaataaagc cgttcaatac
    26161 attgctgatg tttttctttt catcatcagt cagcttacca tcttcaatag ctgttcttac
    26221 agtatctatt aacgaacctg tcgttgttgc cacttgtcca attgacttca atgtaacatc
    26281 tacactgttt gtgatgcttg gtatcgcatc tttcagctga gaggcacctg attttgtttg
    26341 gtttgctaga tcacttgcct gattttctaa atttttcaca tctggcaaga tatcttgtac
    26401 ttgttggatg atcgtcaagc cttgttttgc ttcatcgatt ccttggttca ttgtctcttg
    26461 gatcgaagca aaatcgccgt cgatctctgc tagttgctta ccggcatttt ggatctctgg
    26521 gattttttct tgcaaatcaa ggatgatttt tgcttgatct ttcaactcag gcattttgtc
    26581 attcaatgca acgacttttt cacccatctc atctacttta gggatgtaat ccacgaattc
    26641 atttgcttta ttcagttttt ccttgatttc aggtagttga ctttgtaatt cgaggacttg
    26701 ttttgtgtag ccgtcgatcg tgtccaaatt ttcatctgtt gataaaatca tatctttaac
    26761 tttattgata cttactaggt tcgtatcgat gtcgtaacct atctcattga atactttcaa
    26821 taaagtcgaa ctggctgttt tgataaattc atttgtgatt tgcgattgga tcgaacttgc
    26881 tcctttatcg gtgattttcg gtgcaatcgc attgattttt tcatttaccg tatattcgat
    26941 ttttggtttc ttgatatcac cactagtaaa actcagcaga tcttctgaga aatcctttgg
    27001 caagtaaata cctgcgtaat actttccgga acggactccg tcttctaatt gttcttttga
    27061 atcaacgaat tgccatccta attgtttatt tttatgaagc gattcgatga cttgctcccc
    27121 aatggccact tctttacctt gaaactctgc tggcttgtct gcactgtaaa cagcaattgg
    27181 cagctctcca gtatttccat atggatccca taatgctttg atgttaaacc acgcatatag
    27241 cgaagggatg atcatgatcg cgatgatcag aagtgttgca atcgggtttt tgaaaatccg
    27301 tttccagtca agcaaatata atcgaagcgt atttttgatt gactgcatag ttctccccct
    27361 tcttcttttt tcagctgaat tactttacca tgtatttcat acttttttca atcggctttt
    27421 ttcaaaaaag tcgaattttt ggacatatca cggcaattgt ctcattgtaa tctcttacat
    27481 attgagaaat aattaaggat tacagccttt actttatcat gaaataaaag ggaaagataa
    27541 ttttatgcca aagtttttat ctattatacg tatacgcaaa aaagctaaaa cattttttgt
    27601 ctttgcccct acaaaaatgt ttcagctttt ttcgtttttt attcctgtat ttcttatttt
    27661 tcgaatgaca ggatgatttt cccagcagga ggatgtcctt caactagctg atgtgcttgg
    27721 atgacatgct taagtgaagc agacagattc attgaaataa aaacatgaaa ggctcctttt
    27781 tggtaggctt tgattatgcc agcaaaggct tctgcatcta gatattcctt tcttggaaca
    27841 aacgattcat aaaagccttc ctttttttgc cgcaaatcta gatctgggag gtcatttaat
    27901 gcaacatacc tgccgcctgg tttcatgatc tggatgccgg tttctccttt gatacttcct
    27961 ttcgtcgcat cgattacaag gtctgcctga tcagcaaatt gcagacctgg atttgtttta
    28021 tcataagcag caaatgctga agctcctaat ttttttacct tttcctcatt tttactggat
    28081 gcactggtta agattcggat tcccttttca tggagaagct ggatcagact tgacccgacc
    28141 gcacctgatg ctcctagtat cattacagta tctgtcggct ggatttccgt caaatggttg
    28201 attagattat aagccgtaat tcctggcgtg accattccgg ctgcttcttc ccaactcatt
    28261 ttatctggaa tttttgctac ttttgctgat ggcaacacga cttcttctcc gtaagtcccg
    28321 ccgactgcat ggacagctac tcgatcacct acatgaacag ttgtgacatc tgaaccaatc
    28381 tctgtcacaa taccagcgcc atcattacct ggtacataag ggaatttcaa tgttcgtatc
    28441 tctttcattt ctcctaaacg caaggcaaca tcataaggat tcacactgaa tgcttttatt
    28501 tctacacgaa catgtccaga accgacttct ctgggatgag catcgatttc ttcaaaaaca
    28561 tcttgtgctt ctccgtaacc gttaattgca aatcttcgca tggtcaccat ctcccttttt
    28621 ctttaagttt acgtttatgc ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca
    28681 tgattcaaaa caaaatagtt gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag
    28741 cgtaaagatt acgatttacg ctaataaaaa ataattggag gaatttgttg atggctaaaa
    28801 aatcaaagat tgccaaagca aaaaaacagc aagaaacgat tgaacgctat gctgctttac
    28861 gccaggaatt aaaagcgcaa aaagattacg aaggcttgcg taaacttcct ttagatgctc
    28921 gtccagaacg attgaaaaat cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta
    28981 aattcgggat gtcccgtata aaattccgtg aattagcaca taaaggtttg attccaggag
    29041 taaaaaaagc cagctggtaa tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga
    29101 ccactccttg gatcatcaaa aaagatacgt tcgcttcttc tagcagaaat gctggtacta
    29161 gaagcagacg tatctttttt tacgcacagc tggtcctttt ttatatgtaa aggctgcatg
    29221 gttacttcgc tatatttatt tagtaacagg agtcagtgct tcgataccca cactaatttt
    29281 caatgctcgg tcaacttctg ccataatggg tcgatccaaa tggcagacct tttctttcaa
    29341 tcttgattta tcgatcgttc tgatctgctc caacaggata acggagtctc tttctattcc
    29401 tgttccagta gctttgatcc caatgtgagt cggaagctta ggtttagcca tttttgccgt
    29461 tatggccgca acaatgatcg tcgggctaaa atggttacct agattatttt gaataaccaa
    29521 aactgggcgt gttccgcctt gctccgatcc cacgactgga gaaagatcag caaaataaat
    29581 atctcctctt tttaccatag gatcctcctt attcttgata ttctcttggt atccgctgtc
    29641 ctaatccgca agccacttca tagtggatcg tacctaattg atctgctata tcctgcatag
    29701 tgatttcttt gtctgcattt ttgccgataa gcgtcacttt tgttccgaca ggaaattctt
    29761 gaggcaatcg gatcatcagt tgatccatac atacacgccc aatcgtttca cagtagttcc
    29821 cttctaccaa tagagaaaaa ccctgcatct ttcgtggcca gccatctgca taaccgatcg
    29881 gtatcgttcc gatccactct gcttcaggag tgatatatgt ttctccatag ccgattcctt
    29941 cacctgctgg gagttttttg acttgaatca attcagaaac cagctcaagt gctggctgca
    30001 atggataaac ttccggtagt gcgtgcccag agggattaag tccgtacatt gctactccat
    30061 aacgaatcat attgccgatt gtttcatcat gccataaagc cgttgcacta ttgctgacat
    30121 gtacatatcg aggaagttcg ggaagttttt tcagcacttc tataaagcgt tctttttgca
    30181 gattccaata tgtatcatct gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc
    30241 attctaatac acgactttct ttgatgaatg cagctgcctc ttttacttct tcaggtgtac
    30301 aaaatccgat acgacccatc ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta
    30361 gcttactttt ctctaaaaca gcagctgctg cttgcaacca ttcccgtgta cctgcagtta
    30421 ctgataaatc atgagcaaca gctacagtca gtgcgtcgta aggaaccata ttcaagataa
    30481 ggatgggctg agtaaatcca gcttctctca actcgactcc ttcatctaaa tttgaaacac
    30541 aaaagcccga agctccacct gcaactgctg cctcagctgt ttcaatagct ccatgaccgt
    30601 atccgttcgc ttttactact gcaaacaatt cttttccttg cggaagtctt gcaacttcat
    30661 ttgctacatt ttcagttata gcttttttat gtatcactgc tttcgttggg cggtgccaag
    30721 cgacaaccat cgtttttcct tctttctagt cttccaaaat aatttgtgca aaagctgttt
    30781 catttgtatg agtaatactg acccatacat tgccttcatg aggtgaacgc gtcacaacag
    30841 gctgaccatt ttcatttttt aaaatctcga catcttgaaa ggtaactttt ccgatcccag
    30901 ttccccaagc tttagagaaa gcctctttgc aagcgtaacg cccgcctaaa aattctactt
    30961 ggcgatgaaa aggcaaggac tggaatagtt tcatttcatc agaggtcaag attcgtgcaa
    31021 tgaattttgg tttttcttcg atcagccgag tgatcctagg aagttcgact gcatcgatcc
    31081 caattccttt tatcatatgc ttttctcttt tcaataaatt tagaacactc tcattctacc
    31141 aaattataac cattttgcca caaataagtc cacaaataac aaaa
//
LOCUS       DASJUF010000024        30168 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJUF010000024 DASJUF010000000
VERSION     DASJUF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.024"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(159..1469)
                     /locus_tag="U4B62_000978"
     CDS             complement(159..1469)
                     /locus_tag="U4B62_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2141151.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2839)
                     /locus_tag="U4B62_000979"
     CDS             complement(1481..2839)
                     /locus_tag="U4B62_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2141152.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3041..4609)
                     /locus_tag="U4B62_000980"
     CDS             complement(3041..4609)
                     /locus_tag="U4B62_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2141153.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..5023)
                     /locus_tag="U4B62_000981"
     CDS             complement(4613..5023)
                     /locus_tag="U4B62_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN2141154.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5020..5943)
                     /locus_tag="U4B62_000982"
     CDS             complement(5020..5943)
                     /locus_tag="U4B62_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN2141155.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5940..6818)
                     /locus_tag="U4B62_000983"
     CDS             complement(5940..6818)
                     /locus_tag="U4B62_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN2141156.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6805..8142)
                     /locus_tag="U4B62_000984"
     CDS             complement(6805..8142)
                     /locus_tag="U4B62_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2141157.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8145..9248)
                     /locus_tag="U4B62_000985"
     CDS             complement(8145..9248)
                     /locus_tag="U4B62_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2141158.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9322..10830)
                     /locus_tag="U4B62_000986"
     CDS             complement(9322..10830)
                     /locus_tag="U4B62_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2141159.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10796..11488)
                     /locus_tag="U4B62_000987"
     CDS             complement(10796..11488)
                     /locus_tag="U4B62_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2141160.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11510..12484)
                     /locus_tag="U4B62_000988"
     CDS             complement(11510..12484)
                     /locus_tag="U4B62_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2141161.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12784..13656
                     /locus_tag="U4B62_000989"
     CDS             12784..13656
                     /locus_tag="U4B62_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2141162.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14114..16312)
                     /locus_tag="U4B62_000990"
     CDS             complement(14114..16312)
                     /locus_tag="U4B62_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141163.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16544..17536
                     /locus_tag="U4B62_000991"
     CDS             16544..17536
                     /locus_tag="U4B62_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2141164.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17596..19017
                     /locus_tag="U4B62_000992"
     CDS             17596..19017
                     /locus_tag="U4B62_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN2141165.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19010..20509
                     /locus_tag="U4B62_000993"
     CDS             19010..20509
                     /locus_tag="U4B62_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN2141166.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20548..21942
                     /gene="uxaC"
                     /locus_tag="U4B62_000994"
     CDS             20548..21942
                     /gene="uxaC"
                     /locus_tag="U4B62_000994"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2141167.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22101..22784
                     /locus_tag="U4B62_000995"
     CDS             22101..22784
                     /locus_tag="U4B62_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN2141168.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22786..23622
                     /locus_tag="U4B62_000996"
     CDS             22786..23622
                     /locus_tag="U4B62_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2141169.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23633..25387
                     /locus_tag="U4B62_000997"
     CDS             23633..25387
                     /locus_tag="U4B62_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141170.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25663..26004)
                     /locus_tag="U4B62_000998"
     CDS             complement(25663..26004)
                     /locus_tag="U4B62_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2141171.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26072..28243)
                     /locus_tag="U4B62_000999"
     CDS             complement(26072..28243)
                     /locus_tag="U4B62_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2141172.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28412..29311
                     /locus_tag="U4B62_001000"
     CDS             28412..29311
                     /locus_tag="U4B62_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2141173.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29382..>30168)
                     /locus_tag="U4B62_001001"
     CDS             complement(29382..>30168)
                     /locus_tag="U4B62_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2141174.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9028 a         6223 c         5346 g         9571 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgcgaaataa aaaaatgcaa
     4681 aaggaaacgc aataaactga gtgccaatca acaatatgaa tcctgttata aaatacaaac
     4741 cagtggcagt tgttaagtat tttcctttag cagtcaaaaa caatgctact gctaaaaaag
     4801 caagtgaaat aaacaacaca acggcaaaca gaccagtcat gtcctgtaat aatccaaatg
     4861 tattttctaa agacaaagtt gaatgatcta cagcaagcga ctgataactt tttttaaact
     4921 ctgacgcaga catattactt aaagtaactg ctaacccgcc aaagaaaatc aaagatagac
     4981 tgccaccgac cattcctagc aatagttccc attttctttt catttctgaa ccccctgctt
     5041 ttctaaatgt atccattcag gtcttttgat cgatgtttca taattgttat tacgttcaga
     5101 gaatttgaca gtctgttcat ttttcttgtc atcccaatta tcccaaccag ttgggtgaac
     5161 atgcccacct atcttacacg cttcaaataa gacatttgca tacggacgcc acggtcttcc
     5221 taaatagacg tttctagtat caggttctgc agtcaaggta cagctttcaa aacgataccc
     5281 tgttttttga tttttcggcg tagatgctgc tgtgatgtat ccaggatttt tactggctaa
     5341 gctatggata tgacactgat gaaaaacggc atctgcccca ccaaatatga aatccaccgt
     5401 tccttcaatc cagcaatatt cataggtctg acgacaatac tcaggattgg cagaagccgg
     5461 tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac ggacccgtgc ataaggtatc
     5521 ctgataacca gatagtcgac agttttttaa agatacgcga tctcctgaat tgaataaagc
     5581 aacagcctgt cctacctttt ctcctggacc tgctgtattt tcgatgtgaa gattttctaa
     5641 tacgacattc gatccttcta agaaaaaagt agcacttcgg aaagtcccta actggctacc
     5701 gtcaagatac tcctgttttg caaaacgatt accggagata actactgttc ctgctcccac
     5761 aagtttaaaa ttggataatc tgctttctat atattcatca tatcttcctt cttttactat
     5821 caagaccttt ggatggctat ccggcaggcc tgccaaataa tcgattcctt cttggatagt
     5881 ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt atcagtttgt ctttcatcct
     5941 cataaatgct cccttatgaa ttgtttccaa atatttctca tttcttgtgt ttccatatga
     6001 ccgcctgtta aagaaaaaga agaaaagttt tctggactac ccattgattg atactttttg
     6061 tccagccctt ttctcagctt cagtacgcct tcgtccaagc acatcgtatc gttttttcca
     6121 actaagctaa gacgagggcg cggagcgatt ttttcttgga ttgctaacgt tgtataagct
     6181 gtcaatagat ttggcacata gtaataaaac ccatgatgat ctaggcggcg atgtagcaaa
     6241 agtatttcga tgtccacctg tgccgctaga tcaatacaaa ctttgacacg agaatccaat
     6301 gctgtcaccc accagcttaa taagcctccc atagaaagac caatcgttgc gagccgttga
     6361 gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc tgatcacatc ataaatacgc
     6421 attccccata acgtttttcc tgttaataaa aattgtttga acaactcgct ttcagaaata
     6481 cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa cagcatatcc cattcctgtc
     6541 agtacttctg caaaagatgg agaagctaag taaggagcac catggatcaa ttcacttttc
     6601 ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga ccggcggaaa cggtccttct
     6661 ttttctggat aagcatagag ggctggtact gtttccaatt catttaagta caattgatag
     6721 tattcaagca taaaaccgtt ttgtttttct cggtataatc gttttccact aggggtatgt
     6781 ctgctagatg gagcacctaa aaaatcattc aactgcataa gcaaagtttt tacgaacaac
     6841 cagttcatcc atattttctg attgatttga ttggaatttc tcaatactga tttctttagt
     6901 attttctaat agaaatgctg gtccctcgtg attttcaatc gacatctggt taaaggaaac
     6961 cttacccgca aagccaatat aaaatccttg tctgctcatt tcctctattc ctgtcatcat
     7021 agcaggaaca ccgggaactg catcttgggc catcgagaca tcgatattgt caaatgtgat
     7081 ttcttgtatg aattgttccg ctaatccata aataaaacct gcagaagcat ggacattcct
     7141 tgccgtgata ttcgaaaaat ggatccttct gaatgctggt gtccgttcat ctatcggata
     7201 agcctttttt tcccatacat acggttcttt ccctcgcgga ccacagaaat aataaagatt
     7261 cagtataaat ggacacatca cattatccat gactatattg gatactcgaa tatcttcaac
     7321 gatcccgcct cgacctcgtc tagatttcaa tcgtattcct cgatcagttt cttggaagat
     7381 acaatttgaa atggtaatat tacgaatact accactcatt tcacttccta atacgacacc
     7441 gccatgtcca tggaccatcg tgcaatttgt gatcgtgata ttttcgcaag cgatcctttc
     7501 gtacgtatct tcagtccctg ccttgattgc aatacagtca tctcccacgt caatatggca
     7561 attactaata cgcacatttt tgcatgattc tggatcaatt ccgtctgtat ttggtgaatc
     7621 cgctgggttc aagatcgtca agttatcaaa cgttgcatta ctgcaaagaa tcgggttgat
     7681 cgtccaactt ggtgactgga tcaatttaat atctttcaca gtaattcgat gacagttatg
     7741 gaaactcatc aatttaggac gaggataagc gagattatct ggttcatttc taaaagtatg
     7801 ccaccatttt tttccatttc catctaatgt accgaaacca gtgacagaga tgttctctac
     7861 attttgagca tagatacagg aagcataaac ttttcgatgg actccttccc aacgtgaatg
     7921 aacaacagga taatcttttg gatcgtcaga gaatttcaga accgctcctg cgcttaaatg
     7981 aagttcgaca ttgcttttta aaaataaagc tcctgttaga aattcacctg ctggaacaac
     8041 tacggtcccg cctccgtcac ttgctgcagc atcaatggct tgttgtattg cttcagtatt
     8101 caattcatca attgatgcgc caaatgtcaa aatatcatac atatttataa tgcctccact
     8161 tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat cattacaaat aattggttca
     8221 ctgatgtagt aagcatatga accatctcgc tgatctgctc cgcctaaccc tgctacttga
     8281 cagtttttat tcaagttcag ccagccttct tttgtttcca gaacaaattc atcaagtaat
     8341 ccttgatgtg atttcttcac ttgtggaagc cattcttctt tgttcagcag tcccaaacgt
     8401 attcctttag ccattgcgta aacaaacata ctactgccgg aagcttccag ataatttcct
     8461 ttccgttttg tttctgttgt tacttgatac catgtcccgc tgttttcttc ttgatatgga
     8521 agtaatgcag ccagtgtctt ttctagaatt tctttcagca aagaacaatc tgtttcttca
     8581 tgaagtaatt cataggtatc tgctgcagcc atcaaatacc agccaatcga tcggctccaa
     8641 aaatgatggg atagacctgt ttttggatca gcccatggtt ggaccttctt ttcatcccat
     8701 gcatgaatca gcaaatttgt ttttggatcg aatagatgtt tataactgat ttcaaactgt
     8761 ctcaaaacat cttggtaatc tttttcttca ctaaaattct ggataaattc agcataaaaa
     8821 ggagaaccca tatataagcc atccaaccaa atttgataag gataaatttc tttatgccaa
     8881 aaagctcctt cggatgtgcg tggatggttt gccaactgct gggaaaggag ttctgctgct
     8941 tttttatatt tttcttcgcc tgtttctttg tacaataaaa agaaaagctt tcctgtattt
     9001 acatggtcga tattgtattc ttcaacgctg tagccacgga tcgttccatc ttcttgcacg
     9061 aaatagtcga tattgtcttt tatgaaatca aaataaattt tttcttgggt tcgttcccaa
     9121 agtaattgga accctttaag tacgacgcca tagtcataag accattttcc tttataccac
     9181 ttttcttcat ataagcgagg tgttctttcc atgatacttt ttgctaattt ctttgaccag
     9241 ttttccatta tttgctctcc tttaaaaatt aaaataggat ttgggacaaa taccaagaag
     9301 tatttgtccc aatcgtctat attactgtgc cgctgctttg tatgcttcct cgtactcttg
     9361 tgtgattcga tcgcctcctt gtgctttcca gtcttctaca gcttttttga atccatcttc
     9421 gtcaagctgt cccatgatgt attgcaccgt agcatcaatg acgatttttt caatttctga
     9481 tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa ggatccatta cagcaaattc
     9541 ttcattttct ttgatcaatt gattagagaa ttctttttca gggtctgtat ctttgaaaga
     9601 gaacgtcact tcactaggac gagagcttga taacggttgc acctcttgtt gccaaagatc
     9661 catatcgata aattcgatag aaccatcatc attcaattca taatgcgttc cttcgatacc
     9721 gccagtcatc aatttataca catcttcttc taaaagatca tcaacaaatt gaagtaattt
     9781 cttcaattgt gcttcgtctt ttacttgaga tttgggaaat gctagcaagc cgccaatacc
     9841 atttccttct gaccaaacat gataaccgcc gccttcacca tctgagatct tgtttacagg
     9901 cactaattcc atatcatctt gaataccttg acccatcgtc ttcagattct tgatatccac
     9961 cattccagtg tagattccag ttcttccttg tgcaaattgt tcttgctgat cagtcttttg
    10021 ggtaactgca aagtcttgtg ccaaatagcc attctcaaat agatctcttg aaaaattcaa
    10081 agctttcata tactcttctg tatcaaattc tggagtgaat ttgccattat cgtctacagc
    10141 ccagccattt ggtgctccaa aataagaagt gaaaagttta aagctggtat aacgaatatc
    10201 actaggcgca cgatcgccaa aaccaactgt gtcgtccaca ccatttccat caggatcgtt
    10261 ttctgtaaat tgtttagcta cttcataaag ctcatccatg gtagttggaa catctagtcc
    10321 tagattatcc aaccagtctt ttcgaatgac taagccagca cgagcgtagt ctttttgtat
    10381 cggtacgccg tacaaggcac cgtcgatact cgcagcttcg attctatctt cagaaatttg
    10441 acttaaattt ttatagtctt ttaaatattc tgatacatcc cagaatacgc ctgattttaa
    10501 cgcattgcgg acagaagagt ttttcaacat cgcaatttga agtgtaacga tatctcctag
    10561 ttcatcagaa gccaatgcag ttgttaatct ttcttctttt gaagcatcag gaatccagtt
    10621 gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc acaatcgtgt ctgtaggcgg
    10681 tgaagcatga tgcaaaatat ttaaccaagt gattttagct tttccatctt ctgtctctga
    10741 agaactgtta cttgaactgc tgccgcatgc ggcaagtgct agaattcctg caaaactaag
    10801 acctaatttt acgagcgttg cttttttcat aaattttctc cttcactgtt ttaataatat
    10861 ttttgttttc ttctgttttt tctgtatatg agttcttcca ttccaaatgg caaaatatag
    10921 aatttgctgt ccacgtgact gcggaaaaga agagactgcc accgataaag gttaaaacac
    10981 ctggcattgt ttggatgatc atcagataaa tccctaccat acttaatatc aacactaatg
    11041 agtaatgaag atgtgagaat ccgaataaaa atgccatttt cattttgaaa aaatatcctt
    11101 tccaatcata atgagccatg attgggaaaa tatagagtgc tgagagcaca tatatgaacg
    11161 cagcaatata caaagcgatc ctaagatacc aattgtgtat gaaagaaaga tccacaagta
    11221 aaacaatccc gatggcgcca tatatccagc tcatcagcaa ggtttcacga tagttttctt
    11281 taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc ttttgagcgc agccattttc
    11341 taatcactgc aaccatcgca tacgatgcag gaccgaaagt aaaaactccc aaacctgcga
    11401 tgactaaacc gccccacaga aaattcaaat aaaccaactg taaaatacga tttagccttc
    11461 cgtttatttt atctaatgac tgcagcatat ctttccctct ctttctatct tagtaaacgc
    11521 cgtcttctcc tagctttttg gcaatcttat tagctaaaat taccataaac aatcctacga
    11581 cccctttaaa caatcccatt gctgtactga aacttaactg tccattcttc aatccagctg
    11641 tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt caatgaattc aataacaagt
    11701 acatatgctc gaaccctaag tctaatgtct gaccgatttt tagaatcagc aaggtaatga
    11761 ttactggacg aattgccggc aatgtaacat gccatgtttt gcgtaagcgt cctgctccat
    11821 ccatttctgc agcttcatac aactgtgtat cgactgcagt tatcgctgaa aggtagatga
    11881 tcgtagacca gcccaactct ttccagatca cttgtccgat atatactgtg cgtgtccatt
    11941 caggggatgt tagaaaactg attttttcaa atcccattgt tgcaaggaat tcattcaata
    12001 cccctccgtc aacattcaaa aatacataag taatcgaaac aatgattacc caagacatga
    12061 agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa tcgtttgttc gttacttcat
    12121 ttaatagtaa agacaaaatg attggcatcg gaaaaaacac gaagatattt aatccgaaca
    12181 agatcaacgt atttcttaat aatgtaaaga atgtcggttc tgtaaacaaa cgaatgaaat
    12241 gtttgaaacc gacccaagga cttcccatta ttcctaaaaa tggttgataa tcttgaaaag
    12301 caataacgag tccccccata ggaagatacc taaaaatcaa gaaataaagg actcccggca
    12361 gaatcatcac atataaaaat ttattcgttt tgattcctgc aattattttt tttcgtcttc
    12421 tgagcttttc ttcttttttc gatagcgtca gttgaaatgg ttcttgtttc tttacacttt
    12481 ccataagccc ctccttagtt ttgtgaatgt ttgctgctta tgacttcatg atagataact
    12541 atctgaatta cgtatataat catttttacc gtaagtaaaa acgcttacat agcaatgatt
    12601 tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa taaactacgc atagcaaacc
    12661 ttctattttt ttctatctga cacttagcat aggaaatgat tatatacgta atctttcacc
    12721 gatgataaat tgaacatata gaaacagaaa catttaattt attttgagga ggagcctatc
    12781 tatatgaaaa agaaagtctc tataggagaa agaagtttta cacttttcaa caatacgttt
    12841 ctaattttgc tagctttgat ctgtatcgtt ccttttttaa atatcatcgc tacatctttt
    12901 gcttctacac aagaagtcgt cgcgaaaaag tttatccttt ttcctactac cttttcttta
    12961 gacgcgtatc gctacattct ttctacgccg accattttta gagcacttgc tgtttcgatt
    13021 ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa cttcactgat ggcctacggt
    13081 ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact ttcttgttgt attttctatg
    13141 ttgttcagcg gggggatgat tccaacgttc ttagttgttc gttctctagg gctggtcaat
    13201 tcttactggg ccatgatcct gcctgtggct gttaatgcaa tgaatatgat cattatgaga
    13261 aactttttcc aagccttgcc ggatagtttg gaagaatcag caaaaatgga tggttgcaca
    13321 gattttggtg tatttttcaa aattatgctg ccattagcgt taccgtctat tgctactatc
    13381 tcgctttttt atgctgttac ttactggaac acttatatga ctgctatttt gtatatcaac
    13441 gattcatcta aatggccgat acagattctt ttacgccaaa tcgtcattgt atcaagtggg
    13501 atgcaagcag aaagtagtgc cgttgatgtg attccaccag cacaaacagt taaaatggct
    13561 gttatcgtta ttgcaacagt gcctatgctg attgtttatc cttttgttca gaaatatttt
    13621 gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat gtactgaacg caaaatggta
    13681 gttgtgtcag aagtggtcag cttcaagaaa taagaaggca tttacgaaaa ttgcttctta
    13741 aatttttgtg aaatttcggc ttatttttcg aagaatctac tcctggaaca ccgtgtatca
    13801 gaggttgtga aagagacgtt cagccttgag cgatgataaa aactctcaca aacattgcga
    13861 cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa tagcttccca tatttcacaa
    13921 aattatgcct tattgtcgat agcctgtctc ttgaacgcca tttattcgta aaaaagaact
    13981 gtgacaagac ttttgtcaca gttctttttg cgtgcttagc cttgaaattt aactatccat
    14041 aaatacaaat actagggtta ttctaacttc ttttattgct tgaatcagtc cacaagaaaa
    14101 acagcctctt gattcacaaa tatttttttc gatattctcc cggcgtaatc gattccttct
    14161 ttttaaagaa acgaataaaa ttttgcggat ttcgatattg caatctttct gcaatctctt
    14221 ttacggagag gttagtctct ttcaaccatt tttttgctac ctctaaacga tgattttgaa
    14281 caaaatcacc aaatttttct cctgtttcct ttttaaatac attacttaaa tagttagggt
    14341 tataatgcaa cctatcagcg atcacatcta aagacagatc acgatcatat tctgtgtaga
    14401 cgatatgcat aatattctcc gacaaatttt taaattcatg atcagtgatt gcttgagagc
    14461 attctgtgat tggcagaact aattgttcta aaacaagttt ttctagttct ttgggatgat
    14521 aattgttcag tgcttttaca taaatttgtt tcatgttatc aaagtttttt gaatctactc
    14581 ccaataactg tcctaattga acgagttcat tgatcaagcg gaccgaaact acttcccgac
    14641 tcaaaggatt tttattttgt gcaaacaatg catcaatcaa ttcatgtact aaattcttca
    14701 cttcctgatt tccagaacga atggcttcaa acaatcgatt ttgttcttct atcggatatc
    14761 taataagtgt tttttcatga gcagcaggaa cgatttcctg ataaaaaata attgaacttg
    14821 gaccagtatt tacacgataa tacagggctt cttttgcccg atcaaccgat tgcttgcttt
    14881 cattcagcgt ttcgaaccgg ttactgatgc ctatactgat agttaaattc aagtattctt
    14941 ttacacgttt ttgaatgagt cggcaatact ccatgacttt tttgcttgct tctgcatcat
    15001 ctttatccat tcgataaata gtggcttgca tttcttcatt caaaacgatc ggcagcattc
    15061 gattttcctt tggcacaatt tccgtgatca tgttgttgat cgctaaaagt agcaaatcac
    15121 gctccccacc gtgactatct tctaacagat cgatttgaac caaagctgta taatagcagt
    15181 cattttgaga actataacca aattgctgca accgctgatc tagttctcgc ctttctactc
    15241 gatttctaaa taaactaagt acaaatagcg tttccagctg tggcttttgg gtaactaagt
    15301 gtgcacttaa cgcttctttt tctcctatga tccgactgac tgattgagcg accaaatcta
    15361 gttctttccc actgaatcct tttgatttaa gattcagacg ttcctgtatt tgagaaatcg
    15421 gacgggcaaa tctgtccgaa aataagtagg aaagaagacc aatgagtcct accagtgtta
    15481 aagacactgc aactagcccg attctcagat tgcggatcat tgcgccaatt gcactagagt
    15541 caacttctaa tctgtaaatc caattattat aatctgattt cagtactata tacgttttat
    15601 ctttttctct tatcactttc agctgattgt aatctagatc cacgtccgtt aatacagttg
    15661 gttgctcttt attttttact gtctgagtag taaaaaggtt gccaacttcg ttttcaatag
    15721 aaagtatgtc ttctccactt tcaccaacaa cttgttctat tgtatgtttc tctatcgtag
    15781 caattcccaa tgcatatttt tctttttcaa acattggcaa agaaatcacc atttcgattc
    15841 ctttgttttt cttttgccaa aataaattat ttttgttatt tatgtacttc tgttgatact
    15901 tttcaatttc ctcttcggtc agttgactca acgatccatt gattactccc catttaccag
    15961 ccaaactgac taaatcataa ttattatcat ccataacaat cgtttctata aaatttagtt
    16021 ctttcctgat ttcagaataa gtggagtaat ctgtatatgt cagcggatgc ttgaatatgt
    16081 ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata ttcaatcgtt ctaattttcc
    16141 attcaacgtt agttttaact tgcctgagca aattcttttt gtctgttaaa taactcgatt
    16201 cgatcgattc ataggtattc tggtaaatga ataaactaaa tcctatgaca agtacggctc
    16261 ctaataaaaa catcggtaaa aatattttat aaaatactgt acttttcttc attttcttcc
    16321 tctcctcctt ttaaaataaa aggctttcat ttttctgtaa aattgatact catctaatta
    16381 aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca agtaattttt
    16441 aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat cgtaatatga
    16501 tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga
    16561 tgtcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag
    16621 tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc
    16681 gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt
    16741 ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca
    16801 ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac
    16861 agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca
    16921 gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat
    16981 caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt
    17041 agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt
    17101 tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc
    17161 tgtcaatcca gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat
    17221 ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat
    17281 agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc
    17341 tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt
    17401 acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga
    17461 aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc
    17521 gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac
    17581 agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct
    17641 gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt
    17701 cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa
    17761 caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga
    17821 ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc
    17881 aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat
    17941 atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat
    18001 actcccccac agagcctccc gggcaaactg acagcctttt tataccgacg tttcaaatta
    18061 aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta
    18121 aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg
    18181 ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct
    18241 cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa
    18301 gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca
    18361 ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt
    18421 aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa
    18481 ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta
    18541 ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct
    18601 gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca
    18661 ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa
    18721 gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat
    18781 cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct
    18841 tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa
    18901 gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat
    18961 caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac
    19021 cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat
    19081 tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc
    19141 gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg
    19201 ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa
    19261 tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag
    19321 tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg
    19381 gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact
    19441 gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat
    19501 tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat
    19561 tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg
    19621 tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt
    19681 gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga
    19741 agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa
    19801 tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact
    19861 tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc
    19921 agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga
    19981 ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt
    20041 ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc
    20101 tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga
    20161 aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc
    20221 ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc
    20281 gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa
    20341 aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt
    20401 agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca
    20461 aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa
    20521 cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac
    20581 gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac
    20641 tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct
    20701 tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa
    20761 aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca
    20821 aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt
    20881 attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt
    20941 ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt
    21001 tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa
    21061 ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa
    21121 ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat caccacctat
    21181 caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt
    21241 gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa
    21301 gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat
    21361 cgtacagaaa cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag
    21421 ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat
    21481 accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac
    21541 cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac
    21601 tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag
    21661 ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa
    21721 tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc
    21781 ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg
    21841 gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt
    21901 agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt
    21961 aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga
    22021 agacggacca aatttgctcc gtcttcttct cttgctgttc tgcttcattg cctataaacc
    22081 tttatgaaag aagagataac atgcctacta tccatattgc cggtgattct actgcggcag
    22141 aaaaagatga aaacaagcgt cccgagacag gttggggaga aaaaatctct gcttattttt
    22201 cacccgagat ccagatcaat aaccaagcaa agaatggacg cagcaccaaa tcatttatcc
    22261 aggaaggaag gttagcccgt ctagcttcac agtttcagcc gaatgatttc ttatttgtac
    22321 aatttggaca taacgaccag aaaatccaag aagaaagagg tacgcttccc ttcggtagct
    22381 atttaaaaaa tctgcagata ttttttgaat gcgcaaaaaa agcgaacgtc caaatggtct
    22441 tactgacatc tgtgacgcga cgagattact tagaaaacgg aacattgaat ccagatatac
    22501 ttggagacta tccaaaagcg atgcgtgcat ttgctgagaa acaccatatt cctcttttag
    22561 atgtgttctc aagatcacaa gaattgttcc agaccttctc aaaagaagag acaaaaaaat
    22621 tctacctgca tcttatacct gatacttgta aaaattaccc agaaggacta aaagacaata
    22681 cccactttag tccagaaggc gctgataaag ttgcccgaat aattgttgaa ttgataaaag
    22741 aatcccgtct gcctcttgct aattatttac aaaaaggagt ttgagatgtt tccattaaat
    22801 ttaggcatcc gtgcccacga tttagatgtt cgaaatcgtg aagaaatcgt tcaaaaagta
    22861 tcacagcttc acttgtccca tatccaattt gcaccacaaa aagcatttcc tgaacaactt
    22921 aaattaactg acatgacact tgggagtgct gcctattttg gtgaatactt ccaaaaaaac
    22981 aatatcgaac tttccatttt aggatgctat atcaacctat ccagcaaaaa tcaagaagtt
    23041 cgtgcacaag cattggcaac tttcaaacag caaattgctc tgtgctctgc ctatcaagcc
    23101 aagatgattg ctacggaaac cggtagtgtc tctgtcggtt acacaaaaga aaattttact
    23161 gaagaagctt atcagatcgc cagaaattct atcattgaat tagtcgctca tgcagaaaat
    23221 tttggcgtaa ctgttgctgt tgaagcaggg atcaatcatc caatctataa ctatcagtta
    23281 gctaaacgtt taatcgatga agtagcttca ccaaatttaa aaatcatttt agattgtgcg
    23341 aatttgatgc ataaagaaaa tcatgctgat caggaaaaag tgatccgagg tgccttggac
    23401 aatttacatg gttatattac agcgcttcat ctcaaagact atatcatgga aaatggaaag
    23461 atccgcatgg tacctgttgg caagggctgt ttagatttcc gcccggtact gcattatatc
    23521 aaatatgagc gtcctctaat gtacgccact cttgaagcta ctcctgagat ttatgttcct
    23581 gaagcaatag aacacttgca gtttatttac caagattgtt aaggagaaga ttatggagga
    23641 acgaatacag aaaagttatc aaaaatatca tgcaaatgct tttcagacat tgctgcatct
    23701 ttatcaggga aattattggc gttttgccat gtcaggactc ttttttatta tcaaacatct
    23761 tccatcttgg ttaatgccga tcgctattgc caatgtaatc aatgcagctt catcgaaaga
    23821 ttcttctcag ttgcaggcca tttatttcaa cgcttttttg atggcttgtc tgattggtca
    23881 aaatatattg acgaattatt ttcatgtaaa ataccatagc acttcgatcc gtcaagtaga
    23941 agctggacta agagcgaccc ttatcaagaa aatccaagaa ctatctattc cttatcaaaa
    24001 agaattgcaa tctggacgaa tccagtcaaa gatcatccgt gatgtagaag ctatccaaac
    24061 gttgtcctct caagtatttg tcagcggatt aaatatcgct gtcaatttat tcgtagcttt
    24121 aggtatcacg ctttataaaa gtcctgttgt tttcatgttt ttcttattga ctgttcctct
    24181 cgccgcattc gttactctct ttttccgtaa aaggatcaga cagtccaact cagacttcag
    24241 gcaagaaatg gaagagactt ctgcaaaagt gatcgaagtg atcgaattag caccgattgc
    24301 tcgagctcat gggttagaaa aacacgaaac aactcgctta aataagcatt ttactcaaat
    24361 ctatgaaaaa ggattccact tagatatcct gcaatctgtt ttcggatcag tcagctgggc
    24421 tagttttcag atatttcagc tgatttgttt gattttcact gggatattag ctttacaagg
    24481 gcgcatccaa cccggcgatg tcgtgatgta tcagacgtat ttcacgacga tcgtcaattc
    24541 cgtttctgga atgatcacgc tgattcctac gatttcaaaa ggcatggaat ctatcacttc
    24601 tattggcgaa ctgttgattg ctggcgatgt agaaacttat cacggtaaga aaaaaatcca
    24661 acatttggat ggaaattttc gttttgagga tgtttctttt ctttatccgg actcactcaa
    24721 accgattatc gatcattttt ctctagaggt gaaagctgga gaaacaattg cttttgttgg
    24781 gccatctggc tctggaaaat ctacactcct taacttattg attggattta tccagccgac
    24841 ttcaggaaaa atttttctag atgatcagcc tttggatgaa ctggatatgc gaagtgtgcg
    24901 aaattattta gccgttgttc ctcaaaccac tttgttgttc tccgcttcga tcaaagagaa
    24961 catcacttat ggcttgaaaa acgtatcaaa agaacgttta gatgaagtca tcgaagctgc
    25021 gcaactttct tcactgattg accagcttcc tgatggtctg gataccttag taggcgaaca
    25081 tggcaacaag ctgtctggtg gccaaaagca acgtatctcc attgcacgag cgttgatacg
    25141 tcagccaaaa atcattttgc tagatgaagc aacctcagct ttagataacc agtcagagaa
    25201 aaaaatccag caagccctga attacctgac agaaacaccc actactttta ttgtggctca
    25261 tcggctttca acgatcaaag aggcagataa aatcgttgtg atcgatgaag gaaaaatcgt
    25321 tgaaataggt acttacgaaa aactgttagc tcaaaaaggc tatttctacc gcttgaaaaa
    25381 tgaatgacaa aaaataggag ctatgacaag acttttgtcg tagctcctat ttctgaataa
    25441 acggtgttct cctgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc
    25501 tgttttttga aattgttcct tgtaactcag agttaaacgg taggctcacg acctcttggt
    25561 aaatggtgtt caagagacag cctatcggca ataagaagca attttgtcat cgctcaaggc
    25621 cgaacgtctc tttcacaccc tctgttgttt cataaaatag ttttattcgc tgtaaaggtc
    25681 gcgaactggt gtcatgatga tacggttcaa gtcattgatg attgtactga agccttgctc
    25741 tttttgcatc aagtcattga ttacttcttc tgattggatt ttcacagcca tttcttgtgc
    25801 tttcattgca tcttcgtcgc tgaactcttg tccactcatt tgtttttctt gtaattcgat
    25861 ttgcatcgct tggaagtctt tgaacagttt gtgtgcttct tcgtttgctt gtactttctc
    25921 gtacgcttct ttcaacgctt tgaattctgg taattcacgg atctcacgtt cgatttgatt
    25981 cgcgctatcg tagatgtttg acatgttatg ccctcttttc ttttagtagt tactctctat
    26041 tgtaacatgg ggttattcgt tttcaaaggg gctactctcc ttgatatttc cgccagaggt
    26101 ctttagccgc ttcaccaatt ccctgaacgc cttctttcac catatcgccg aagtttttca
    26161 gcccttcttt tgcttgttca ccatattgat tcaagttatc ttgccaattt ccagactctc
    26221 cattagaatt tcctgtgttt ccaacttgat ctgcagctac cacttgtcct cctgtggcat
    26281 aggcatctgc tacactaaat cctgtttttg aagtatacgg aaggatgcct tctgccgctg
    26341 atttgaagac tttcccgaca acatttccac ttgtcccttc caaatagtgc agcttgctgg
    26401 attcttcgta acccatccat gtcgaaatca ctacgtctgg cgtgtaaccg atcatccatt
    26461 ggtcatttgc ttttgtggca tcgaaattag tttctgtggt tcctgttttc cctgctatcg
    26521 tataacctgc aggattcgca gcagccgctg ttccatttga aaatgtacca agaagcatgc
    26581 tggtcatttc atcggctgtt tcctttgata tgacctgttt tggttttttg tttgtgttat
    26641 cgacgatcac cgctccagtc gaatcaacga ttttcgtgat aagatgcggc gtatacatct
    26701 gaccatcatt ggcaaatgca ctgtaagcac ccgccatgat caatggagat actccttttt
    26761 ccagtccacc aagggctaag ccataatact tatcagattt tgctaaaggc aggccgaatt
    26821 cttctgcttt atcatatcct ttttgtaaac cgatctcatg caaaagccat acagtaggca
    26881 aattcaaact ttgtgcaaca gcttgataca tcggtacttc tccttggtat gtcccgtcaa
    26941 aattatgcgc atcgtaataa ctttgcggtt cgtctttcaa gatactggaa ggcttgtatc
    27001 cagcttccag tgctggtgta tatacgctta atggtttgat cgttgatcct ggtgaacgac
    27061 gcatctgtgt agcaaagctg aatcctctaa agacatgatc tcctcttcgt cctactagtg
    27121 cttgtacacc acctgtattc ggatctaaag ctactgaagc agattggacc atggcaccat
    27181 ctgaagcatt tgctgggaat aatgcactgt tttgataagt agcttctaat tgcttttgat
    27241 aatcctgatc taaagctgta tagattttat agcctttatt catgacatct tcttcatcaa
    27301 gaccatactc gttaactgct tcatcgatca ctgcatcaaa ataataagga taacgataac
    27361 cagaatctga attatcgtat gtgtcattaa gatacgcgct gatatctgtc tggctttctg
    27421 cttgttcttg ttcttttgtg atttttccgt tttcttccat tacacttagt actgtgtttt
    27481 tccgatcatt cgcatttttt gggtgatcga tcggattata aataccagga cctttcagca
    27541 ttcctgccaa agtagcagcc tcagacagcg taacattcgt tgcatctact ccaaaatact
    27601 ttctggaagc gtcttggaca ccccataccc cgttgccaaa ataggcattg ttaagataca
    27661 tggtcaagat ttcttctttg ctgtacttct tttcgatctc gattgccaaa aataattcct
    27721 tcgcttttcg atcaaacgtt tggtctaatg tcagatacgc atttttggca agctgctgag
    27781 tgatcgtact tcctccgccc cctgatgttc cgccagacaa gaccattcgg actgctgcac
    27841 gggcaatccc tttgatatca aacccgtgat gctcgtaaaa attcctatct tctgtcgaga
    27901 ttactgcatc ttggatatag ggagaaatcg cgtttaattc tacatatgtt cctttttggg
    27961 cgtacagtgt gccagcttct tcgtttttct tatcataaat gactgtcgat tggcttaatc
    28021 ctgattttaa cgtttcgaca tttgcttgtt ttgctaaaat aaagaggtag atacttgtca
    28081 ctaagacaca gatcaaacca atcagcaaaa tcaatttgtt cacatgatat tttttccaga
    28141 tgcgctttcg ccatcgatgg aattgtccga gatatggtct gatccaacgc caaaaactga
    28201 tcaaagcagc ttttatcttt tctaaaatgc tttgaaaatc catttgcttc ttctcctgtc
    28261 tatttgattc aatcgtctta ttttaccata gtgaaagagt gttttcttcc tgcttaagtt
    28321 gtagttcaat atgttttttt acgttagtgc aagtagagta acataagaat gaacagaaaa
    28381 atgtaagttt tttcttaaat atgttttaaa aatggtacaa ttagtctgcg attgcactga
    28441 aaggagtaca cttgtgaaaa tcacgatcac tttacctatg aaccatcctg aaacaacaat
    28501 tcgcgacttg cttgaaaagg aatggttagt tcctagaaag gttcgacact ttttgcgtat
    28561 acgtaaaaac gtttggatca atcaacaacc tgctttgttc catcatgaag taaagggcaa
    28621 tgaccagatc acattgttat tcgaagaaac agattataat tatcaagaag ttcaattagg
    28681 agatgcgaag cacatccgtg ttttatatga agatgagcat ttgattatag tggataagcc
    28741 tgcaggaatg aagactcatc caaatgaacc gacagaaaac agtaccttac tcaatcattt
    28801 agctgcttat ctcgacacta aacagcaacg accttacgtt gtccaccgct tggacaaaga
    28861 aacaagcggt gccgttttat ttgcaaaaaa tccatttgtc ttgccgatac ttggaagaat
    28921 gttggaacaa aaaatgatct atcggagata tcaagccatc gtatggggag aaattgccaa
    28981 agatatgacc atcaccaata aaattggacg agatcgccat gatcggagaa aaagggtcgt
    29041 agatgataaa aatggtcaga gcgccttgac acatgtcact gtcaacaaag tactttctga
    29101 acaaacacaa gtctattgtg ttttggaaac cggacgtaca caccagatca gagttcattt
    29161 agctcataag ggacatccga tcgttggaga ccctctctac caaaatagaa cagctgaacg
    29221 actgatgctc catgcattag aactgcacat gatccatcca tttactcaag aaaaaatagt
    29281 cggtaaagct ttacctggat tatgggaata gaaataaaaa agaacagagt cttatcagca
    29341 gataatgata agactctgtt ctttttgtgt aatttgtctg cttacttttg atgttcgtgt
    29401 tctgctaaca gcaaactacc gataatccca ctttcattcg gcagtcccca acgaacgata
    29461 tattcactta cttgaggtgt ttccatataa ccacccatca attcaacaaa ttgctgacgg
    29521 attttttgaa ggagatgatc ctgattcatt accccaccgc ctaaaataat cttttcaggt
    29581 gctaacgtca acgtatagtt gatcagtgct tgagcaatat aataagcctg aatagcccaa
    29641 acaggatgat cttgcggcag atcttgccct ttgatccctg tgcgtgcctc aatagacggt
    29701 cctgctgcta atccttctaa acaatcttta tgataaggac aagtaccttc ataatggtca
    29761 tctggatggc gtttcaccca aatatgtccc atttcaggat gtgcgatccc tgaaaaaatt
    29821 tcttcattga tcacgacacc tgcaccgatt cctgttccca cggtaaggta aatacaacta
    29881 ttttttcctt gtgctgctcc ttgcttcaat tccccatatg cagcagcatt gacatccgtt
    29941 gtccaagcag caggaatatt gtagcgttcc ttgattgtgc caagaaaatc gaattgtgcc
    30001 cacccttttt tgggagttgc caaaacgtat ccgtaattat cttttgtttt gtcgatacca
    30061 atcgggccga atgagccaat cccgattccc ttcaattcat aacgatcaaa aaattcaaat
    30121 acctgatcca atgtttcttc tggtgttgtg gtagggatac ttaatttt
//
LOCUS       DASJUF010000025        29470 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJUF010000025 DASJUF010000000
VERSION     DASJUF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.025"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            55..924
                     /locus_tag="U4B62_001002"
     CDS             55..924
                     /locus_tag="U4B62_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN2141175.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1089..2360
                     /locus_tag="U4B62_001003"
     CDS             1089..2360
                     /locus_tag="U4B62_001003"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2141176.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2357..3652
                     /gene="rho"
                     /locus_tag="U4B62_001004"
     CDS             2357..3652
                     /gene="rho"
                     /locus_tag="U4B62_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN2141177.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3751..4014
                     /locus_tag="U4B62_001005"
     CDS             3751..4014
                     /locus_tag="U4B62_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN2141178.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4459..4908
                     /locus_tag="U4B62_001006"
     CDS             4459..4908
                     /locus_tag="U4B62_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2141179.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4965..6383
                     /locus_tag="U4B62_001007"
     CDS             4965..6383
                     /locus_tag="U4B62_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2141180.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6383..7243
                     /locus_tag="U4B62_001008"
     CDS             6383..7243
                     /locus_tag="U4B62_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN2141181.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7417..8190
                     /locus_tag="U4B62_001009"
     CDS             7417..8190
                     /locus_tag="U4B62_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2141182.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8337..8702
                     /locus_tag="U4B62_001010"
     CDS             8337..8702
                     /locus_tag="U4B62_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN2141183.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8850..10058)
                     /locus_tag="U4B62_001011"
     CDS             complement(8850..10058)
                     /locus_tag="U4B62_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2141184.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10077..10355)
                     /locus_tag="U4B62_001012"
     CDS             complement(10077..10355)
                     /locus_tag="U4B62_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141185.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10509..11204
                     /locus_tag="U4B62_001013"
     CDS             10509..11204
                     /locus_tag="U4B62_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2141186.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(11646..12443)
                     /locus_tag="U4B62_001014"
     CDS             complement(11646..12443)
                     /locus_tag="U4B62_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN2141187.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12440..13624)
                     /locus_tag="U4B62_001015"
     CDS             complement(12440..13624)
                     /locus_tag="U4B62_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2141188.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13649..14896)
                     /locus_tag="U4B62_001016"
     CDS             complement(13649..14896)
                     /locus_tag="U4B62_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2141189.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15111..16856
                     /gene="dnaX"
                     /locus_tag="U4B62_001017"
     CDS             15111..16856
                     /gene="dnaX"
                     /locus_tag="U4B62_001017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN2141190.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16876..17190
                     /locus_tag="U4B62_001018"
     CDS             16876..17190
                     /locus_tag="U4B62_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN2141191.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17215..17811
                     /gene="recR"
                     /locus_tag="U4B62_001019"
     CDS             17215..17811
                     /gene="recR"
                     /locus_tag="U4B62_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN2141192.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17875..18519
                     /locus_tag="U4B62_001020"
     CDS             17875..18519
                     /locus_tag="U4B62_001020"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN2141193.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18534..18863
                     /locus_tag="U4B62_001021"
     CDS             18534..18863
                     /locus_tag="U4B62_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN2141194.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18877..19815
                     /gene="holB"
                     /locus_tag="U4B62_001022"
     CDS             18877..19815
                     /gene="holB"
                     /locus_tag="U4B62_001022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN2141195.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19851..20675
                     /locus_tag="U4B62_001023"
     CDS             19851..20675
                     /locus_tag="U4B62_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN2141196.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20668..21015
                     /locus_tag="U4B62_001024"
     CDS             20668..21015
                     /locus_tag="U4B62_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN2141197.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21084..21956
                     /gene="rsmI"
                     /locus_tag="U4B62_001025"
     CDS             21084..21956
                     /gene="rsmI"
                     /locus_tag="U4B62_001025"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN2141198.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22065..23186
                     /gene="dinB"
                     /locus_tag="U4B62_001026"
     CDS             22065..23186
                     /gene="dinB"
                     /locus_tag="U4B62_001026"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN2141199.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23240..23842)
                     /gene="nrdG"
                     /locus_tag="U4B62_001027"
     CDS             complement(23240..23842)
                     /gene="nrdG"
                     /locus_tag="U4B62_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN2141200.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24157..26346)
                     /gene="nrdD"
                     /locus_tag="U4B62_001028"
     CDS             complement(24157..26346)
                     /gene="nrdD"
                     /locus_tag="U4B62_001028"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN2141201.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26645..27439
                     /locus_tag="U4B62_001029"
     CDS             26645..27439
                     /locus_tag="U4B62_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141202.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27429..>29470
                     /locus_tag="U4B62_001030"
     CDS             27429..>29470
                     /locus_tag="U4B62_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141203.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         9440 a         5307 c         6172 g         8551 t
ORIGIN      
        1 gttaagaatt gtcttaacaa atttagtttt acaaaactta ggaggaattt tattatgcca
       61 gtagtatcag gagctgaatt tttaaaagct gctcgtaaag gcggttacgc tgtcggcgga
      121 ttcaacacaa acaacttgga atggactcaa gcaatccttg aagcggctga agcaaaacaa
      181 gcaccagtac ttatccaaac atcaatgggt gcagctaaat acatgggtgg ttacaaaatc
      241 tgtaaagatt taatcactga tttagttgaa gcaatgaaca tcactgttcc agttgctatc
      301 cacttagacc atggtgatta cgaagctgct ttggaatgta tcgaattagg ctatacttca
      361 gtaatgttcg atggttcaca tctaccattt gaagaaaact tgaaattagc taaagacgtt
      421 gttgaaaaag cacatgcaaa aggtatctca gttgaatgtg aagttggttc aatcggcgga
      481 gaagaagacg gaatcatcgg taacggcgaa ttagctgata ttgaagaatg caaacaaatg
      541 gttgctacag gtatcgacta cttagcttgc ggtatcggta acatccatgg tcaatatcca
      601 gaaaactgga aaggattagc ttttgaccac ttacaagcaa ttgctgatgc tgtaggtcca
      661 gacatgcctt tagtattaca tggcggatca ggtattcctc aagatcaaat cgaaaaagct
      721 atctcaatgg gtatctcaaa agtaaatgtt aacactgaat tccaattatc atttgctgca
      781 gctactcgta aatatatcga agctggtaaa gataaagaag gtaaaggatt cgaccctcgt
      841 aaattattag ctccaggtaa agcagcaatc gttgaagatg ctaaatctca tatcgactgg
      901 ttcggttcag ctaacaaagc gtaagcatcc acataacaga tacgatatat aggaatggag
      961 aacgactttt gaagtcgctc tccattcttt tttacttaaa acggcagacc atgctcgttt
     1021 ctttttttta tctatgataa aatacaagca tatccatgaa aaaagattaa taaacaaggt
     1081 ggaaacagat gaaaaaaatc gtaatcaaag gaaaccgacc tttagctggt gaagtaacga
     1141 tcagcggtgc aaaaaatagt gcagtcgcat tgattccagc tgcaatctta gctgattccc
     1201 cagtgatctt ggaaggtgtg ccggacatac aagatgttca ttcattaatt gaaattttag
     1261 agatcatggg cgcaactgtc catttttcca acaacatact agaaatcgat cctcgagaag
     1321 tcgtatcgat tccgatgccg cacggaaaaa tcaatagttt gcgtgcttct tattatttta
     1381 tgggaacact gcttggtaaa tttggcgaag cggtagtagg cctgccaggt ggctgttatt
     1441 taggccctag accgattgac ctacacgtca aaggatttga agctctaggt gctgaagtga
     1501 caaatgaaca tggtgccatg tatttacgga cagaaaataa aggattgcgt ggcaatcgga
     1561 ttttcatgga cgtggtttca gtcggagcaa cgatcaatgt gatgttagct gctgtaaaag
     1621 caaaaggaca gacagtaatt gaaaacgctg cacgagaacc ggaaatcata gatgtggcaa
     1681 ctttactgaa taatatgggg gcaaaagttc gaggagcggg tacagatgtg atccgtatcg
     1741 aaggagtcga aacattacat ggctgtcggc atttcatgat tcctgaccgc atcgaagcag
     1801 gaacctatct agcattagca gctgcagtag gaaatggtat caaagtgaaa aatgtgatct
     1861 ttgaacattt ggaaagcttt atagcaaaat tgcaggaaat tggtgtccat atgaaaatca
     1921 gcgaagatga aatcgaagtt tacccctcga aagaattaaa acccgcaaac atcatgacct
     1981 atccttatcc aggatttgcc acagatctgc agcagccact tactgcgtta ctattgatga
     2041 ctacaggcac ttcagaaatc atcgatacga tctatgcgaa acgtgtgaac catgtgccgg
     2101 aactagcacg aatgggcgct gatattaccg tggaaggaaa tatgatcatc gtgaatggtc
     2161 caaataaact tcacggcaca gaagtcgtgg cttctgatct tcgggcaggt gcttgtttag
     2221 tgattgctgg actattggca gaagggacaa cgacgattta caatgtagat tatatcttac
     2281 gcggttatga tcatattatc gagaaactaa cagccttagg ggcatcaatt gaaatgatag
     2341 aggaagtggg agctgaatga gcgattattt gacaatggca gaactagaga acaaaacgtt
     2401 aaaagaagtt tatagttatg ccaaagaatt taaaattcct tattacagta agatgaataa
     2461 aaaggaatta tctctagctg tgattcgtgc acaagcagag aaacaaggat tttattatat
     2521 ggaaggaata ttggatatcg ttggtcaaga tggttacggt tttttacggc caatcaatta
     2581 tggaccaagt gcagaagata tctatatttc tgcctcacaa atcagacgat tcagtttgag
     2641 aaatggggac aaagtggcag gaaaagcccg tccacctaaa gaatcggaac gttattatgg
     2701 tttgatgcac gtggaaagcg ttaatggaaa agatccggaa gaagcaaaag aacgtccgca
     2761 ttttccagcc ttaacacctc tttatccgca aagacagctt caactggaaa caacaccgca
     2821 aagactatct acccgaatga tcgatatctt ttctccaatc ggttttggtc agcgtggatt
     2881 gatcgtcgca ccgccaaaag cagggaaaac ttctgtatta aaagaaatcg caaatggtat
     2941 caccgaaaac tatccggaag tagaattaat cgttctactg attgatgaac gaccggaaga
     3001 agtaacggat ctagaacgaa gcgtgaaagg cgacgtggta tcttctacat ttgatctgca
     3061 gccgcaaaac catactcgag tttctgaatt agtattggaa cgcgcaatgc gtttagtcga
     3121 agataaacga gatgtcgtta tcttgatgga tagtattact agacttgctc gggcctataa
     3181 cttagtcgtt ccaccaagcg gacgtacatt gagtggcggg attgacccag ccgcattgta
     3241 taaacctaag aaatttttcg gggcagcaag aaacatcgaa gaaggtggca gtttgacgat
     3301 tttagcgacc gctttagtcg atacaggaag ccgtatggat gacgtgatct atgaagaatt
     3361 caaaggaact ggaaatctag aattacaact ttcacgtgaa ttatctgaac gtcggatctt
     3421 cccagccatc gatatcaaaa aatcaggtac ccgaaaagaa gaattattga tgccaagtga
     3481 agaattagaa gaaatttgga aacttcgaaa aaatatgact ggagattcat tagaatatac
     3541 ggaacaactg atccgctttt tgcgtaaaac aaaaaacaat cagcaatttt tcaaagaatt
     3601 tcaagatgtc tcctttggta aaaaaaatcc gaccagaaat ctgaaaagat aattgcaacc
     3661 ttgactgaaa catgttaaga tgttaatgtt gtaaatcgac aaatcaactc tggttcagca
     3721 aatggagcag ggcaaaggag cgaaaaaatc atgaaagaaa acatccatcc agattaccac
     3781 ccagtagtat tcatggattc aacaactggt ttcaaattct tgtcaggttc aacaaaaact
     3841 tcacaagaaa cagttgaatg ggaagacggt aatacatacc cagtcatccg tgtcgaagta
     3901 acttctgact cacatccatt ctacactgga cgtcaaaaat tcacacaagc agacggacgt
     3961 gtggaccgtt tcaacaaaaa atacggtctc aaagacgcaa acgctgcgga ataataaaaa
     4021 cgctgataac tcaacgttta gcagactatc tgatagggat agtctgtttt tttatgtttt
     4081 ttgccaattt aaaacttcgt ttgatgctga aaaagtcgta ggatcataat gcacaaaaca
     4141 atgaaaaact agtatatagt tttaattatc cacttgataa aaacacctta tattaacgaa
     4201 atgaagaggg gttatttcga agtaaaaaac catttaccta aaattagaag ctaagctttc
     4261 ctttcaagga aggctttttg ttttgttata gacttgcgat tgtttgtgag ctagatcgtt
     4321 cattttaatg acttatacac gcggatgatg ggtctttttc ctctgttcat tcataatatt
     4381 gaacggtaca gctagcgatg ctatactgac ctcagttcca agaaaacgtt aacatttttt
     4441 gtgggggaga ggttttttat gaggtttgat caattgatcg cagaagaact gattgttttc
     4501 gatccatcga tagattcaaa aggatcatta tttgagcgaa taggggatgt gttgaaaaaa
     4561 acaaataagg taagaaatgt tcaaaaagtc gttaaggcac tattcaaacg agaggaggag
     4621 atctccacag gaatagaaga tggatttggg attccccatg caaaaagcaa atatgtgaca
     4681 aaaccagcac tgtgtttttt tcatacagcc aaattaacgg actatagcgg gatagatgaa
     4741 atgccaattg aatatatttt tgccatcgta gttcctgaaa aatcagcaga tatgcatcta
     4801 gagattctca gcacactctc cagacgattg atgaatgcag attttagaaa gggaataaaa
     4861 gaagccacta cgccagcaga tatcatcaga cagattagtc aagaatgaaa atagtagagt
     4921 ctttgtccga gtatcgatag tgaagaaaaa ggaggaaaat aatcatgaaa atcgttggtg
     4981 taactgcttg cccgactgga attgctcata cttatatgtc ggcagaaaaa ttagaagtaa
     5041 cagcaagaaa tctaaaccat caagcaaaat tcgaaacaca aggtgtaaaa acagaaaatc
     5101 agttatctga acaagaaatc aaagaggcag atgcaatcat tcttgcagta gataaagaaa
     5161 tcgaattgga ccgatttgcc gggaaaaaag taaagcgtgt ctctacaagc cgagcaatca
     5221 aagaaccgca agtagtaata gacgaagcat tgagagatat tggcgtattc gttgtgtcta
     5281 atgaaaaaga accagctgca aatgagaaaa agccgacaat ctataatcat ttcatgaatg
     5341 gtgtgaatta catgcttcca tttgtgatcg caggcggaat catcatcgca ctgagttttg
     5401 cctttgggat tacagcggct gatcccaact cagcagacta taatgtgtta gcagctgcat
     5461 tttcaaggat cggaggagat acagcgtttg ccatgatggt tcctgcgtta ggcgcgggga
     5521 ttgctacatc gattgctgga aaagcaggtt ttgccccagg aattgttgcc ggattactgg
     5581 cctctacagg tggttcaggt tttcttggcg gaatgattgg tggcgtgcta gcagggtata
     5641 tctgtgattt tctggcaaat aaaattagcg taaaaaaaga attttctgct atttatcagc
     5701 tgattgtcgt ccccttttta tcgatcttaa ttattgggct tttgatggta tttgtgattg
     5761 aacaaccgat cacatgggtg ttggatgctt tgacaaattg gctgaatagc ttgggaaata
     5821 catccgggct attgttcgga ttacttatcg ggatcatgat ggcagctgat atgggtgggc
     5881 cgatcaataa atcgacttcg actttctcga tcggtttgat gtcagctgga gtaaatgcac
     5941 cgattgctgc ttgtatggct gcaggaatga cacctcccct aggtattgct ttagccactt
     6001 tattattcaa acataaattt accaaagaag aacggacttc aggtcaatca tgctggattt
     6061 taggcgcttc gtatattaca gaaggtgcta taccgtttgc ggtatctgac ccgatccgcg
     6121 tgatacctag tttgatgaca ggttcagcag tcgctgcagg gatttccatg gcagtaggag
     6181 ttacttcaat ggcacctcat ggagggattt gggtgatgct aattccaaat gttatgaaca
     6241 atttacctat gtatgtcttg gcaattgcat caggtacagt cactacagcc tttatgcttg
     6301 gactattgaa aaaaacagtg aaagagaaaa aacaagacaa gtggacgaaa aatgaaaatc
     6361 taacaaatga ggtggaagcg taatgttata tacaatgaaa gagttattga aagtggctaa
     6421 agagaatcaa tttgcagtac cagccttcaa tatttgcagc tatgatatga tgaaagcaat
     6481 catcgaagaa gcagagagat tagaagcacc agtaatctta gaaattcatc ctgatgaaat
     6541 cgcctattta agtgatgagt ttgttgcggc agtaagagcc tatgcttata caagtaaact
     6601 gccgatcgtt atccacttgg atcatggcgg tacgatagaa gatgtcttac gggcgattcg
     6661 aaacggctat tcttccgtga tgatcgatgc gtctttgaaa tcattcgacg acaatgtaca
     6721 gattacgaaa aaagtggttg cgttagctca tgcggtaaat gtctcagtgg aggctgagct
     6781 tggaacgatt gggaataatg gttctgctga aggaggtgct gaaacaatca tttatacaga
     6841 tcctgatcaa gcgaagattt ttgtgaaaga aacaggaatc gatacacttg ctgtagcaat
     6901 tggaacagcg catggcttgt atccgaaaaa cagaactcca aaattaaata tcgacttact
     6961 gaaagaattg aaccaacaaa tcgatcttcc atttgtttta catggcggtt caggtaatcc
     7021 ggacgcagaa gtcagcgaag cagtaaaata cggagttgcc aaagtgaatt tgagctcaga
     7081 tcttaaaagc gtctttttca atgctttgag agaaatattg agtgaaaatc catcgatgta
     7141 cgagccgaat gcaatttatc cagcagctaa taaaaaggta caagaagtgg tcaaacataa
     7201 aatggaaatt ttgaacacag taggcaaggc tagtttatac taaaaataaa aatgtgtggt
     7261 caagagggta tgacaatagt cttgtcatac cctcttaacc acacacgatg ttcagaagta
     7321 gttttttcga aaaatttctt cttatttctc gaagccaatc acttctgtca caacctcttt
     7381 tttcctgtta ctatataaag taaatggggt gattttatgt tgaatgaagt ggagaataga
     7441 cagcaggaga ttgtgaatta tctaaaaaac aaccaatttg caagagtcac cgatttgatc
     7501 gagttagtaa attatagcga agccacgatt aaacgtgacc ttgtttttct ggaaaaaagt
     7561 ggattgatcc gtagagttcg cggtggcgcc atgcttattg acaatcgaaa aattgacgtg
     7621 ccttacctga tgaagatcac caaattagaa gaagaaacag aaaaacgtaa gattgcagat
     7681 acagcttctt ctttgattcg agatgatatg gtcttattct tggattccag tacaacctct
     7741 ttacacttag tcagaaacct agcaagattt gatggtttgc agatcattac gaatggcatc
     7801 gtcacagctg ctatgttgag tgaatataca acggcaaaag tatctgtatt gggaggggca
     7861 attgttccga aacgagcaac aatcaacggt tcaaaagcct ataacgatgc attgtcatat
     7921 tatgcggatt tagcgataat gagttgtcgc gggctagatt tcagccaagg agtaacagaa
     7981 actcatgaag gagaggcaat ggtcaaaaaa gcttttagaa aacaatcaga ccggcttgcc
     8041 gttttagtta cacagcaaaa aattgcacat aaattcatgc atcaagccat tgcttttcat
     8101 gaaatcgatt atctggtgac aagtaaagaa ctgacaaaag aacagtatgc gaaagtaaag
     8161 gaacaccata tcgaatgtct gtacggataa attattacgt gaatgttcta agtggcttcc
     8221 tcatttttag ataggacctt atctttgctc gcttctctca gccgatggta taataatgaa
     8281 aaggttatct tgttagataa tcttactttt ttatccagga gaaaggagtg cagaaaatga
     8341 aacatgtgaa ggcgttggta gtgaaggcaa tcatgatctg ggcgattctg tgggtcgtgc
     8401 tgacaggttt atacggtgtt agctttatgg attcaacgat tgttggggta attattgtag
     8461 tcatgatcta tgtgctaggg gatctgttga tcttacgtaa agtagggaac atcgcagcga
     8521 cgatcgcaga tgctggttca gctgcagtta tcttgtggct ctatctatat tttatgaatg
     8581 atacagtcga tatttggatg aaagtattga ttcctgctct attgatcggt gtggcagaat
     8641 ggttcttcca caagtggttg ctaagtcaag gcatcgtacc agatgaacgg aaaatggaat
     8701 aaatcaacag ttaaactatt cttatcgata aatgaaagct ttaaaaataa atagatgttt
     8761 aattaagaat aatcataaac tatttttact caaaaaaccg ctcagattat tttcagatct
     8821 gaacggtttt ttttatctat tcatcaagat caaatttcat ttttcaatga ttcaatcaat
     8881 tcgccctctg cttcatgact atgttttcgg ccattaacaa gatagagtgc tgtacatatc
     8941 aatggaatga ctccggcaat cacatacaat cctctaaatg aagtaagaga ttttagcatc
     9001 cccatcaaat aaggaccaac gcctaagcct aggtctaaac caataaagta ggtagataaa
     9061 gcaatactga ttcgatgttc gcggacgatt tttaaacaaa cagcctgtcc atttgacata
     9121 aacgtaccat aacctaggcc gactaatgct ccagagataa gcaatgtcac actattcgtc
     9181 gttacactca gtaaaaataa tcctgctgtt aagaataaat agctaggata catcacgtat
     9241 tgttctcctt tgacatcgaa aatccttcca gtcaatggac gtgtaaatgt gatgaccaaa
     9301 gcatatacga cgaagaaaaa tgacccagca gcaactaagt ggattacttt tgtataagaa
     9361 gataaaaatg ataaaacact tgaataagaa agacccatca aaaaggcaat tcctgtaatg
     9421 aaaagtgctt tcttttcaat aaaactattg atattccatg agcgaagaga tattttttgt
     9481 tcttcagata atggaatatt ttttacagga aagacaaggc aggcaatcgt aacaaagaag
     9541 accagtacaa ttgaaaacca gataatgaaa cgaaaatcag tcatattcag caaaatcatt
     9601 cctaaaaatg gcccaacagc ggcagctaaa ctagtactta atccataata attgatgcct
     9661 tccccttttt tagatgcagg gatatatgca gtaacgatag catttgtagc tgtagaaact
     9721 gtaccgtagc cgaatccatt caagaatcga actaaataca atgccccgat agttggcgta
     9781 taaagatatg ctaatgttga aaggagatag aagatcgcgc cgcctcttaa ggtaaacttc
     9841 cgaccaaata gttctaactg tttccccatg aataagcgtg ccagcaatgt tccgatgata
     9901 tagatcccag aagcaaaacc tgcttggctc aagctggcgt gtaaggtgtc ttgcgcaatg
     9961 actgccatga tgaccatcaa cagataataa atcaggtaga caataaaatt gatcaatgtg
    10021 attccaataa aatatttatt aaataatttc ttttccatgt atgttataca ttccccttaa
    10081 gctgaatatg cttttaattt ttgtatttga acttctacat cgtgataatt ggatggatag
    10141 cgatcaacaa gttggcgaat aaataaatct cttgtagaca taaacagatt taaaaattct
    10201 tcgcattcgt ttgttggatt ctcatccact gctttgatca attcgatcga gttttgatac
    10261 gcatcattta aaaagactaa agaagctttc atatcaaatc ttgtttgata tgctgtttct
    10321 aatcgattaa tgattgtgtt catccagtaa ttcatgtttt ttccctctat tcttaaaaaa
    10381 ttttacttct gcagtataac gaataaaaaa aaggaaaaaa atcgcataag tgatggaaaa
    10441 aggtacttaa caaatgttaa tatcattagt aaaatacctt tggactttaa aaagttttaa
    10501 agtactgtat gatcttttta gatgaaagtt tggatggggg agcatcattg gatagtagag
    10561 tcttaaagga atatttggat aaaaatgatt ttccgattat acgcaaagga tatcataaat
    10621 atttgacatt cgaagggatg gaagattccc atacgtatat cttgaaagag ggaacggtaa
    10681 aagcaagtgt tatttcaaat gatgggagag aatttaacct tcgctatatc aaagatcttg
    10741 aaatcgtctc tcttttaaaa gacgaatatt cacaattcat cgattcgccg tataatattc
    10801 gagttgaatc agaatatgca gagttttatc gaatcaatcg tgtgcgcttt tggcaaaata
    10861 tcaaccaaga cccaaagcta caacactatg taaaagagta ttaccgctat cgtctgcttt
    10921 attcgatgaa aaaaatgcag caaatgctat taaacggaaa gctgggagct gtttgtacgc
    10981 aactttacga attatatgat ttattcggtg tgcggacaga agaagggatg ctgatcgatt
    11041 tttcgatcac gaatgaagaa atcgccaaat tttgtggcat ctcaactgct agcagtgtaa
    11101 acagaatttt aaaacaactg aaaaaagatg gtattatcac cgttaaagac catcatatcg
    11161 ttattttgaa tatggacttg ttagaagaca atattatctt ttgatgaaca aagaggttgt
    11221 gaaatcagcg ttttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    11281 tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttac caagaggttg
    11341 tgagcctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt
    11401 tgatgaatat tgtgacttgt gaccgaggag ttggtaggag aacaccgttt accaagaggt
    11461 tgtgaaaaag ctgttctgca tcaagtaata agaagagcca agtaaaaaca gtctcttatg
    11521 tttttaactt ggctggactt atcaccgtag atgtaagctt ttgaacgccg tttatttgca
    11581 acaaaagggg ctgtgacacg atttttgtca cagccctttt tttgcgaaca ttcatttagc
    11641 aatttttaga aacttctgcc gccaccgcca aaagtcccgc caccgctaga atgggtagta
    11701 cttccgccac cgccgcttcc aggaggtggg cttttgggga ttcttcttgt cgtgacaaaa
    11761 gaatttgtca gacgatcggt tttttcagtt aatttgatac tggatttttc tcggaatgga
    11821 tatttgtaga tgcctgattt caattgataa cgcgaaacag tcacaaaata aaagacaaga
    11881 ctaaggataa gagccaaaac aacagcaatg atgatttcta ctgtcgtcaa aaccttatag
    11941 cgtgtaattt tgccagtttc ttcgtctaca cgataatgtc ctcctggaac tccgtcttcc
    12001 acatatgttg ctgatttaga caggtaagct tttgcagcat tgtaataggc agcatttgtc
    12061 atctgatcat aaacatcatc taagatcgaa tcgattctgc tatcagtcaa ataatcgatc
    12121 atattaccag aagtcgaaat gtagatttca cgctgtccca tatctaatag caatactgag
    12181 ccattttgat catttccgac tgcgtctctt aagtaattat cagcaaagtc tcttggttct
    12241 tctgaattag atgtagtcgt tacgatcaag actcgtcctt tgattttttc attcagcttt
    12301 tctgcctgtt gttccaaaga ttggatttga tctgttgtga ataacccagc ctcatcattt
    12361 accgtagctg tatctgcatg agtagccacc ggccagccaa cacttactag caaaagaaag
    12421 aataaaaaaa attttttcat catatcaagt aacctccgat cagaaagagg atcgctagcc
    12481 cagcgaaaat cccaagagcg gtcagtccca attttttctg gctgatcggc aggacaccgc
    12541 tgacctttcc tgtttgtcca ttcattgcgt agtagtagac ttttttactt gagtcattgc
    12601 ttcgataagt aacaagccat accggcagta atacgtaatt atttacttcg tttgtgattg
    12661 aagcagaagt tcgcacaccg gtcaatgttg tatatccatt tgcggtatct tttaacagct
    12721 tctctgaata gtcttttaat tcagattgaa tctgtgattt gattgcctca tattcgatat
    12781 ctcttttttc tgcttggaac ccggctaaat actggctttt aaaatcaatt gctttatcta
    12841 acgggaaagg ttgcacgcct tcgaccatct tttgttgcgt attttttgat aaggcatttt
    12901 ttactagttc tttaaaagaa agcttgcctg ctcttgaaac agcaaattgc ttcgtttctg
    12961 tgtattcgat gtcacctatc cgccatatgc ggataactgt accattcgcc tgcatggcac
    13021 catctacttc agcatctgta gcccaataag ggaaatacac acctgtcaac ttatcgattt
    13081 gatccttatt gaaaaaagct ttgggaacaa accatttttt cttggtccaa gcgagaaatc
    13141 tgttgattgc ttcttctttc tcgacagcaa aaggaagtac tttatttggc aagaattctc
    13201 cgcttaaacg tcccgatagt acgacgggat tatggcagta gtaacaataa gtcgcagcag
    13261 ttgtcgcatc agtcacgatc tctgcaccac agttgggaca aagaaaaaga tctatcgctg
    13321 cggtattatc tgcagattcg tccgctgtat ctgaaaaacc gcctgcctcg tcaaccgctt
    13381 tgcgctcggc ttcattcata tcggcaagct gttcttgggc tgtaaaagta aaatcttcgg
    13441 taggtgaagg ttcttctttg ctttcttcgc cagccatacg cgcttctttt tgcttttgct
    13501 catattggct gacttcttct tctgtataga tattcaggca gtattcgcaa tgaaattttt
    13561 ggtctttggg atcaaaagtc aacggaccgc cgcaattagg acatttatgt gtaagaacat
    13621 ccatcagctg cacacctttc taaataaatt aattaacggg gattccttta aacggtttac
    13681 cacagtttgg acagaattta gggatgccgt tggaaagatc cacgatctca ttgcattcac
    13741 tgcatcgcat ttgtagctta ggctgaacgt tttctacagg tttggcagct ccgcagttgg
    13801 aacagaattt tccggaattt tctgtcccgc aaactggaca tgtccaagta tccgcatttc
    13861 cttgtgcagt ttgttgattt tgtttttctg cttgttgttt gatttgttct tggttatttt
    13921 gagctgcttg agaaagataa gaaccatttg cattcattcc catacctaca cccataaagc
    13981 cggtcattgc tcctgcttca ttttttccgg ctgcttccat accacgtgca atggaacctt
    14041 gcacgtagcc ttcacgtacg ctaggatcac ctagcatcgc cccttcgtta cgcatattga
    14101 tcagtttcac agaatcatcg gtgtaagaaa tgctggctac agccacagaa acaatctcca
    14161 tcccacgtaa ttcacgccaa gaatcatcca atgcagtatc catgtatttg cttaattcca
    14221 aactcttaga tggtacataa gaaatccgtt gaccatctgc agacatctga ttgattgctg
    14281 attgtagcgc tgttaggaat tcagctaaat actgctcatt gatatcattg atttccactt
    14341 gtgttttgtt tttagggatt gcgtttgtat aaaataaaat cgggtcaacg acatgaatag
    14401 aataagtgcc atgtgcccgt aagaataatt ctgcgttata gaaattgtca aagtagttca
    14461 acggcgcgct tgtaccgaat ttgattccct ttatttcttg gagattgata taaaaaactt
    14521 gttgtttctg cggtgtgacg ccgccaaatt tgaatcggtc aaaagtttca gcaattgctt
    14581 ctttcagtgt tccattgaac atggaaggag ctgcgtcatt tttaatggtg taataacctt
    14641 cctcagcggt ataatcgatg atttttccac catcgaccaa aagcatcatc atgtttggat
    14701 agacatggac gactgttccg tctgttaaaa catcttctgt tcctttacga ttggagccac
    14761 gtttgtcatc tttgcggact ttgacacctt ttgtcatgac cgttgtatca ctcatattgt
    14821 ctggttcgat gacttctagc cattgatctg ccagaccgcc gccaaccata cttgttgctg
    14881 ctttgataag acccataaaa aattcctcct ctgattcagc tagtgccaaa caattattgt
    14941 ttatttatta taccatagga agatgtgaaa cttctcggac tagagacgga attgaaatgg
    15001 aaaattgcgt attgtttaaa tacctgaaaa agccgagaag gatagtagtt tttttaccta
    15061 atatcacgta taataagaga tagtgaattt tttaaaaaag ggggatcctt atggcatatc
    15121 aagcacttta tcgagtctgg cgttcgcagc gtttcgaaga catcgttgga caaaaagctg
    15181 tgacacagac actaaaaaat gctattgttt cccacaaaac ctcacatgcc tacttgttta
    15241 caggacccag aggaaccgga aagacgagtg cagcaaagat tttcgcaaaa gcaatcaact
    15301 gtccgaacag taaagacggt gagccatgca acgaatgcga aatgtgtcga tcaattactg
    15361 ctggtacgca agaagatgtg atcgaaatcg atgctgccag caataacgga gtcgaagaaa
    15421 tccgatttat ccgtgatcgg gcaaattatg cgcctacaaa ggcagcgtac aaagtgtaca
    15481 ttattgatga ggtacatatg ctttcaacag gagctttcaa tgccttgttg aaaacattgg
    15541 aagagccaaa agaaagtgtc atattcatct tagcaacgac cgaacctcac aaaataccag
    15601 ctacgatcat ttcacggact cagcgatttg attttaaacg gatcaatacg gcagatattg
    15661 tcgaacatct tgaatttatc ttgaaagaat cgactatcag ctatgaacca gctgcattga
    15721 atgtgattgc ccgttctgca gaaggcggga tgcgggatgc attgagtatt cttgatcagg
    15781 caatctcgtt tagtgaagga acaattactt tagatgatgc catgcaagtg acaggtagct
    15841 taactgacga aatgatggac agttacttga gtgcttgcgt gaaaaaagaa gtaccagaag
    15901 cattggaaac attgaaccaa attctcgcag ccggcaaaga atcacgtcgc tttctagagg
    15961 atatgttaca atactgccgt gacttgctga tgtatcagca agcacctaac ctagttgagg
    16021 aacaatcgag tacgatcaga agtacattta cagaattggc acaaactgtt tcagcggatc
    16081 agctgttttc aatgataaag attttgagcg atactcaaaa tgaaattcgt tttaccaata
    16141 gtgccgctat ttatttggaa gtcgctacag tcaaacttgc acagctaccg tctgcttctg
    16201 ttccttcagc agatgtagca tttgcagatg atcagatagt aaaacagcta caacaagaga
    16261 ttaaggaact aagaaacgaa atcaaggaac tacgcagtaa tacaggaatt gagaatacaa
    16321 aagtacagcc ggtacagcaa acaaatcata aaaaacagac aagtacctat cgtgtaccga
    16381 cggaacgagt atataaagtt ctgcgggaag cgacaagaca gcatctgaac caagtaaaag
    16441 aagtatggga agacttgctg atgagtctat ctgtcactca gcgagcaatg cttaaagcaa
    16501 gcgaaccagt tgcagctgga cctacaggac tagtgattgc gtttgattat gagattgttt
    16561 gtcaaagagc agcaaatgac gaagaattgc agctttcggt acacaacaac ttaagccgaa
    16621 tgatcaaaga ttatgcgcct gatcctgttt atattacaag agaaagctgg ccagaacttc
    16681 gaagaaatta tttgacgcag gcacaagaag agggaacaga aaactctttt gcttctgaag
    16741 actcagaagg agaaatcgag ttgcttcctc aagaacaaga agtaccagaa gaaacacttg
    16801 tagtaaaagc agaggaactg tttggctccg aagctgttac agtggtagat gattaataaa
    16861 aaggagtaga taattatgat gcgtggaatg ggcaatatgc aaggcatgat gaaacaagta
    16921 caaaaaatgc aaaaagaaat ggcagaagca caagagctgc taaataaaac agagtttaca
    16981 ggtaaagcca ccaatgaatt ggtcaaagcg gtttttacag gcgatcgtcg catgaaagat
    17041 atccaaatca atgaagaagt cgttgatcct gatgatatcg agatgcttca ggatcttgtc
    17101 gtaatggcag tcaatgatgc gatcgcacaa attgatgtgg aaactgagaa aaaattaggg
    17161 aaatacgcaa aaggtttgcc aggattttag ttttaaactg aaggagtaat gaatatgcaa
    17221 tatccagaac caattgcgaa actgatcgac agctacatga aacttccagg aatcggtcag
    17281 aaaacagcga caagactcgc cttttatacg atcgatatga aagatgaagt agtaaatgaa
    17341 tttgctaaat ctttgttaag tgtaaaacgc gatttgcatt tttgtagtat ctgcggaaac
    17401 atcacagaag aagatccctg cgaaatatgt agaaatcctt cacgtgatcg aagcgttatc
    17461 ctagtggtcg aagagccgaa agatgtaatg gcaatggaaa aaatgcgaga atatcacgga
    17521 ctttatcatg ttctccatgg tgtgttatca ccgatggaag ggacaggtcc agaagacatc
    17581 aatatttctt ctctgttgca gcggctgcat gatgcagatg taaaagaagt gattatcgca
    17641 acaaatgcaa caacagaggg agaagcaaca gctatgtatc tctcacgtct gatcaaaccc
    17701 gcaggtatca aagtgactcg tctagcacat ggtctgtctg taggctctga tatcgagtat
    17761 gcagatgaag ttactttatt gaaagctgtc gaaggaagac gtgagttata aatttctaat
    17821 taagtgtttt ttcagataca atataaaagc agcacaagag atgggggcaa caatgtgaac
    17881 ggactattta ttacaatcga ggggcctgat ggtgcaggaa aaaccagcat cttgaatgaa
    17941 ctttaccctc ttttgaaaga agtagcaaaa acagaaatca ttcaaacaag agaaccagga
    18001 ggcattccga ttgctgaaga gatccgagca gtgatccttg atccaaaaaa tgatcggatg
    18061 gatgaacgta cggaagcact gctttatgca gcagcaagaa gacaacatct agtcgaaaag
    18121 gttctgcctg ctctggcaaa agggaaaatc gttttatgcg atagatttgt cgacagttct
    18181 cttgcttatc aaggtgcagg ccgacgcatc ggcgttaccg agattgctcg gttgaatgag
    18241 tttgctacag aaggtactac gccggatttt accctttatc ttgacgtaga ttcagacacc
    18301 ggattaagaa gaatcaagaa aaacagacaa aatcaaatcg accgtttaga ttcggaaggt
    18361 ttggaattcc accagcgtgt acgtcatgct tacttaaagc tggcagaaga gaatccggaa
    18421 agaatccaca aagttgatgc aagaaaaagc tttgaagaag tgctgcaaac aagttatcat
    18481 acaattattg aacagtatcc gcaatttttt gaaaactagg aaggtgaacg tttatgaaaa
    18541 ttattttagc gattatccaa gacaaagaca gcaaccgttt atcaaatgaa ttgattgatg
    18601 caaatatccg tgccacaaaa ctatcatcga caggcggctt ccttaaggct ggaaacagta
    18661 cgtttatcgt gggtattgaa gatgaccgtg tagaagaagc attggaaatc atcaaaaaaa
    18721 cgtgtgaatc aagaaagcaa tttgtatcta cacctgttac actggatatt tcgatggatg
    18781 ggggcgtccc ttatccagta gaagtagaag taggcggtgc cacagtcttc gtcttacctg
    18841 ttgaaggatt ccatcaattt taggaggagc tgagctatga atcaagaaat aaacttggct
    18901 caaatgcaac cgatcgtgta tcagcagctt caacggagtt ttgagcatgg gcgtatcgct
    18961 catgcttatc tttttgaagg ggagaaagga acaggaaaac atgaagtagg tatatggctt
    19021 gcacagcacc tgttctgtac ccaaatgaaa gacaatctgc cttgtggaaa atgcaacaat
    19081 tgtcagcgga tcgaaaagaa agaacatcca gatgttttgg ttattgaacc ggaaggacaa
    19141 accatcaaag tcgatcagat cagacgtttg cagacggaat tcagtcgcag cggttacgaa
    19201 tcaagaaaaa aagttttttt gatcaaagaa gctgaaaaaa tgaatagcag tgcggcaaac
    19261 agtttgttga aattcttgga agagccccct ggagattttt tagcggtgtt agaaacagat
    19321 gcagtaggaa gaattctccc tactattcaa tctcgttgtc aagttctgca ttttcaagaa
    19381 ctttcgaaag aagcactgat ccatcgattg caagaagagc agattccatt ggaaaaagca
    19441 aaacttttgg ctttcttaac aaacagttta ggaaaagcag ttgaaatctc tcaagatgaa
    19501 tggtttaatg atgctaagga cttgatttat caatggttcg tttacttgca aaagcaagat
    19561 actcaagcct ttatctatgt ccaaaaaaaa ttggtaaaat catttaaaga aaaaacacaa
    19621 cagtttacag gattatctgt cttgatgttt tattatcagg aagcacagcg tcaagcagta
    19681 gcaaacggcg aatatggcaa agtgaaacag ataaaccaga taattgaacg gatattgatc
    19741 gcagagcaga aactacgcag caatgtcagt tttcaggctg ttgcggaaca gttcgttttg
    19801 catacgattt tttagcttgc cgcaaaggat gatgatcaag gaggatggaa atggtagaag
    19861 tagtaggtgt ccgatataga gatgcaggtc atatctatta ttttgcacca ggagactctg
    19921 aatatgttta tagtgacaag gtattagtcg aatctcagca atccttacat attgctactg
    19981 ttgtccttcc taaaaaagag attgatccca atgaccttcc tgaagctatc cgtccgatct
    20041 tgcataaagc gtcacaagct gaattagaaa aagacaaaaa aaacaaaaaa gatgcttatg
    20101 atgcattgaa aatcgcaaaa gaaaaagtgc agttgcgtga gttggaaatg aaaatggtca
    20161 aagccgaata cacatttgat cgaaataaat tgattttttc tttcacagcg gacgggcgaa
    20221 ttgactttag agaattggta aaagatctgg ccgctgtatt tcgtactcgt attgagcttc
    20281 gacaaattgg tgtccgcgat gaagcaaaga taataggcgg aattggtcca tgtgggcgtc
    20341 cattatgctg ttcttctttt ttaggcgatt tcgtgcctgt atcaatcaaa atggcaaaag
    20401 atcaaagttt atctttgaat ccaacaaaaa tttccggttt atgcggacgc ttgatgtgct
    20461 gtctgcaata tgagagcgaa gaatacgaga cagcgaaaaa agaactccct gactttggaa
    20521 aagtaattgc tacacctgat ggtaaaggga aagtcgtagg gttgaatctg cttagccgta
    20581 tcgttagtat tcggctgact ggacgggaaa atcctgtcga atatgaatgg gatgaattga
    20641 aagaatttat ccaagtaggt gagatgaatg gataagagat cattatacga cggattgaat
    20701 caattagaat cagagttgag aaatacatta acagaacttg ttgaaatgaa gcatgctttg
    20761 catgagatcg tagaaaaaaa tacaactttg gagatggaaa atcaacgtct gcgggaacac
    20821 ctgcgggaat tgaaccaagt tgcacaagct ccagctgaaa atgtgaagca ggaactttct
    20881 aagtctcgta tgaatcttga aaaaatttat gaagaaggat tccatgtctg ctacgattta
    20941 tatggatcaa gaagggacaa tgacgagcct tgtgcgtttt gtctagaagt gatctatcgc
    21001 gaaagcggtc gatgaccaat aacaagagga gactggagcg gagaaatact gctccagtct
    21061 ttttattaag gagattgtga cagatgtata atcaaaaaag tttcaaggga aatccggtat
    21121 atggggattt atatcttgta ccgacgccaa ttggaaattt agaagatatg agctacagaa
    21181 gtgttcgtat gctgcaagaa tccgatctta tcgcaagtga ggatacgcgt aatacacaaa
    21241 agttattgaa tcattttgaa atccatacac cccaaaaaag tttacatgaa cataattata
    21301 aagaacgtgt ccctcaattg attgctttgt taaaagaagg aaaaacgatt gctcaagtca
    21361 gtgacgcagg aatgccttcc atcagcgatc ctggacatga gctagtttta gcctgcataa
    21421 aagaagggat ttctgtgatc tctatcccag gacctacagc tgggatgacg gctttgattg
    21481 cttcagggct agtgcctcaa ccatttttat tttatggctt ccttccacgt aagaaaaaag
    21541 aacagaaaga aacattgaaa aagctaaaag aacagacagt tacgctgatt ttttatgagt
    21601 caccttatcg gatcactgct acattgacga atttcttaga aatttttgga aatcgccaag
    21661 tcgttttgtg tcgggaattg acaaaggtcc atgaagaata ccttagagga agtacggaag
    21721 agctactcgc ttatatcgaa gaacatccag taaagggtga atgttgtctg cttgtagaag
    21781 ggaatttgtt cgaaatgcca gtgacggaag ttgatgaaga gttgggaacg ttaaaggaac
    21841 aagtagaagc aaagatcgct tcaggcgaaa aaccaaatgc tgctatcaaa gcagtagcgc
    21901 tgaaaaatgg gctgaaaaaa caagaagtct accgtcagta ccatgaatta gactgagata
    21961 gtataagttg tctataatga aagactaatg aggaaatcag ctactaactt ttcaaatctc
    22021 ttgaacgcga acgaatgttc ttgtataatg aataagaggt gaggatgatg gagctgcaat
    22081 ttcctttaca taatgataca tcaagaaaaa tcattcatat tgatatggat gcttttttcg
    22141 cttcggttga agaacgagat catcctgagt ttcgcggaca tccattagta attgccagac
    22201 accccagtga cactggtggg cgtggagtag tgactacagc aaattatgaa gcaagaaaat
    22261 tcggaattca ttcagcaatg agtgcacaaa aagcgtatga gctttgtccg caagccatct
    22321 ttaagcctgg cgaccatcat aaatacagcg atatttccaa acaagtacgg gaagtattct
    22381 atcgttatac agatattgtg gaaccagtta gtatcgatga agcgtatctt gatgtgaccg
    22441 aaaataaaat caattgcaag tcagccatca aaatcgcccg aatgatccaa gcagatattt
    22501 gggagagtgt ccagctaaca tgttctgcgg gagtaagtta caataagttt ttggccaaat
    22561 tagcatcaga ctatcaaaaa cctagaggaa tcacggtagt tgcccctcaa gacgctgtgc
    22621 catttttaaa agctttgcca attgaaaaat ttcatggggt agggaagaaa actgtaccta
    22681 gaatgcatga gctagggatt tatacaggaa aagacctata tgaatgcaca gaaatgatgt
    22741 tgattcgaaa ttttggaaaa atgggctatt ctctttatcg taaagtcaga ggaatccatg
    22801 attctccagt aaacgttaca agagagcgaa aatctgtggg aaaagagcat acttatgggc
    22861 agccgttgca aacggaagaa gcagtgctga ctcagttgcg ccaactagct gaaaaagtag
    22921 aagaagcact gagaagagtc caaaaacatg ggaaaacagt agttttgaag gtcaggtaca
    22981 cggactattc tactgtgacc aaaagagtga cattgccaga atatatctat aaaaaagaag
    23041 cactctttta tcaagcaagc cttatatggg aggaaatctt gggagtagaa aaaggaatcc
    23101 gattgttagg gattactttg acgaatctgg acccgatgac ttatgaaaac atcgtccttc
    23161 cattatggga aaatcaagaa atataaaaac agctttgaga gttgaaaccg gaaatccgtt
    23221 ttctctccca aagctgtttt tatatcaagt cttgcttttt gatttgttca taggtttctg
    23281 ttgcatccgt acatttttcc caaatcacaa cttgattgga ttctaaagat ttttggacgt
    23341 caatgatccg ctggttgcta cttccgcgaa attgtagttt taggtcacgt ttgcttaatt
    23401 caaagcgtcc gtcaacaagg atatcgattt gcgaaaggag ttctaattta tcttctgaat
    23461 cttgtagcaa ttcatcaaag gtatagcctg tccatgacca gatatctttt gtcgtaccga
    23521 actcttttcg gatacgtcgt acgacttgca gacaaacatc tgtattcaaa aaaggttcgc
    23581 caccaagtaa agttaatcct tgcacgtaat cgttgcgcaa gtcagcgatg atttgttctt
    23641 ctaattcagg tgtgaatggt ttgccatatc gaaaattttg tgcagctttg ttgaagcagc
    23701 cttcgcaggc aaataagcag ccactaacgt aaagactgtt gcggacacct tcaccatcga
    23761 caaaattaaa cggtttgtag tcagcaatat agttttggct gtagtctttt gctaaccatt
    23821 cttttggttt tggatttctc ataaaaaaac ttctttcatt taaggttcaa agatatagct
    23881 attgaagata aaatctactc gaccacataa ataagaacaa actgacctaa atccattgtt
    23941 caagaaagct tgttcttatt ttgatagagg gcgttcaaag atacaaagcc atcgaaagag
    24001 ggtggtgaaa aaagacttgt catagcctct tattctgaat aaacggtgtt caaaagcttg
    24061 catctgcagt aataagccca actaaacgaa aaatatgagg aactattttt gtttagctat
    24121 tcttattact tgatgcagga cagctttttc acaacctcat ttcatgtgtt tcacacgtga
    24181 ggaaatttct ttatggcggc catgtaccat cggacgagct tgcggatttc ctaaatagcc
    24241 acaagtacgt ttgactacat cacatgtttt tggatcatga tttccacatt ctggacattc
    24301 gaaccctcgt tctgtcggtt tgaaatcacc ttcgaaacca cattgatagc aatgatcgat
    24361 tggcgtattc gtaccaagat acccgatttt gtcatatgca aaatcccata cagcttctaa
    24421 agctttcgga ttttgttgga gcatagggta ttcacagtag tggatgaatc cacctgaaca
    24481 atattttgga taatccttct caaattccaa tttttcaaat ggcgttgggt ttttacgcac
    24541 atcatagtgg aaactgtttg tataatattc tttatctgtg atattttcga tgatgccgaa
    24601 tttttctgta tccaaacggc agaaacggtc agttagactt tcacttggag ttgaatagac
    24661 actgaaatga taaccatatt catcccccca tgcatctgca ttagctttta attctttgac
    24721 gatatcaagt gtgaaagctt ttgcttctgg gtttgtttcc cattcgccac cgaagaaagc
    24781 tgaagcaact tcatacaatc caatgtagcc taacgataca gtagctcgac ggtttttgaa
    24841 taattcattg acagaatcgg tacgtgaaag acgtttcccg aaagcaccat acatatataa
    24901 aattggcgca tttgctggaa tggcttcttt acaacgttct acacgataga gaagcgcatc
    24961 tttcatgatt tctaagcggt cagacagtaa ctgccagaat ttattctgat ctccttttga
    25021 ttcgatcgcg attcttggca aattcaatgt aacaacacct aaattcattc gtccgacatt
    25081 tacttcttga ccattttcgt ctttccaccc ttgcaagaat gaacgacagc ccataggtac
    25141 tttgaaactt ccagttaatt caacgatttt atcgtaattc agaatatcag gatacattct
    25201 tttcgttgca cattccaatg ctagttgttt gatgtcgtaa ttaggatctt cagggtttaa
    25261 gttcacacct tttttcaaag tgaagatcag tttagggaag atagctgtcc gcttttcact
    25321 ccctaatcca ttgattcgaa tctgtaaaat tgctttttga atttctcttt caaaccagtt
    25381 ttctcctagt ccaaatccta atgaagtgaa aggagtttgt ccatttgaag taaataaggt
    25441 attgatttca tattccaaac tttgcatagc atcgaagata tcttttttgg tttttgcttt
    25501 tgcatattct ttttggcgtt ctggcgaatc gatccattct tccgcgtctt ttagatgttt
    25561 tttatagttc aattctgcaa aaggagcaag caattcatct gttcggtcgg cagaacagcc
    25621 accatattga cttgacgcta cattggcaat aatctgtgag atttgtgctg tggcggtctg
    25681 gatagatttt ggcgattcga cttcggcatt gccgattttg aacccattgt tcagcatgcc
    25741 tttgaaatca atcaagcagc aattcgtcat cggtgtataa ggatgataat ccaaatcatg
    25801 gtaatggata tcgccttttt gatgcgcatt tgctatatgt ggtggcaaca ttttcagtcc
    25861 gattgatttt ccaacgatcc cggctgtcag atctcgctgt gtattgaaaa cattactgtc
    25921 tttatttgcg ttttcgttca cgacagtttg gtccttgttg attagctttt caatcgaaaa
    25981 gttgatatct gttgctttac tgcgagcaaa atctcgtttg gtacgataat tgatgtactc
    26041 ttctgctaat tcatattcgt ttttttctag taatatatgt tccacgatgt tttgaatctc
    26101 atagattttg acattttcag caaaccggct tttgatttct tggacaacca agtctgcaat
    26161 catttgaatt ttttcataag taagtggtga taatgagccg tgaattttct tttctgcttt
    26221 gatcagtgct tcattgatct tttgttcatc gaaatcaaca tggcggccat cgcgtttgat
    26281 gactttcatc tgggatgtct ctggctgctg agtaacgaca gttttttctt cataatttac
    26341 tttcattatc ttcaactcct gctaaattgt tcttgtctat cataaaacaa aatcggggaa
    26401 ggagcaacta aatatcggct atatatagta ttcaatgtgc tgtataattg gatttctata
    26461 ctatatgtag tgtatatttt attgtgaaaa aaggtgaaaa cttgtagctg gtggatttgt
    26521 tttttgcata tttaattttg ctgaataaaa taattgaata aaatttttta ttcagtccaa
    26581 gtaaaagtga caaaaacata agagcaaatt attcgtagat atgctactat gcaaataaat
    26641 agaaatgaaa aatgaaagag gaggagaaga attggaaaag atgatcgaag tgaaacatct
    26701 aagtaaaagc tacggcagca aactttctcc cgtcaaagcc ttagataata tctcattcac
    26761 agtagaagaa ggcgagtttg ttgggattat gggcccaagt ggtgcaggaa agacaacgct
    26821 gatgaatatc ttgtccacga tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa
    26881 agaaatcaca aaaatgaaaa acagtgagct cagtgacttt cgtcgaaaaa aactaggatt
    26941 catttttcaa gagttcaatt tgatcgatac cctaaatgca aaagacaata tcctattgcc
    27001 cctagcagtt gaacggatga ctaaagagga aatggaaaag agagtaagac atgtagctca
    27061 attgttgaat atagaagaac tgctcaaacg gtatcccgat gaactttctg tcggacagag
    27121 acaacgaatt gcagctgcta gagcacttgt tgttcagcca caagtgattt ttgcagatga
    27181 accaactggc tctttggact cgaagtcggc tacagaattg ttgaactact tgaagaccat
    27241 gaatcaaaaa gaaaaagcaa cgatactgtt agtcacccat gatccatata cagcaagcta
    27301 ctgcgataga attttattta ttaaagatgg tgtgattttt tctgaagttg tcagaagagg
    27361 gacaagaaga gagttctttg agaaagtaat agacatgcag gcgacgattg gcggaggagg
    27421 aaaaatgaat gctgtataaa ctagccgtta aaagtttttt tcggcagtca cgaggctatc
    27481 ttgtttattt tttcagtttg actttatccg taatgatcta ttattccttc agcgcaatga
    27541 cctacgatca atctttagtc agaagagcaa gtcaagatac ttcgatagat ggtggattga
    27601 gttttggtgg atttatcatt actgttgttt tgcttttttt cgtattcagt gccaaccgat
    27661 ttttcttgaa tagaaggcaa aaggaaatag gcatctatca gctttttggt atgaataaac
    27721 tgcagatatc aggtatatat gtgttagaaa tcatgataat tggattgttc gcctgtattt
    27781 ctgggatatt actcggtatt attttttcca aactcttttc aatgattttg gttcgaatga
    27841 tggatatgga tcttactagt ccatttttta tctccattcc atcagtagta gatactttat
    27901 ttgctttttt catcatcctt ttagcagtat ctgtatattc aatatggaaa atatggcgct
    27961 atccaatgat tcgaagtttt ggagaaaaag aacaagcaga tagttcaaca atgcgcttca
    28021 ggacaaggca ccgattactc gcagtcatcg ggttactact tttaacgagc ggatattttg
    28081 gagctagtca ttttagggaa atcacttcat ggatgatcag ttatcattat cttgatacat
    28141 tagttttctt ttttcctttt ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt
    28201 tctgttatac atttcggtta tttttgatca tattgagtaa atggaagatt tattactatg
    28261 gtatcaattt tttgataatg ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa
    28321 attctctgat tacagtcatt ctcggaattt ctttaatgac aattggcggc acggctagta
    28381 tctctactat tttatcctac caggagaaag tttcctcccc gatggactat cagtttgatg
    28441 ttgagacaga gaaaaaaatc aagccgattc tagcagagga agggcagaag ataactaaag
    28501 aaatcacctt gcattataaa gtcattggga gtatctatag tcaaaatctc gaagaaccta
    28561 tgtttcaaac tgctaatctg atcacggaaa cacaatatca agccttcaga aaagttaatc
    28621 cagatttgcc gaacatttct ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc
    28681 aaactctttt tcggaatatt tcattatatg gatctagtat tggactgcca gataatcaag
    28741 aattgaagat tcaacaaatg cttccaagtg tattaggtga cgaaacaatg gtatatctaa
    28801 gtcctgtact gattgtagat gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag
    28861 tagtcaatgt agatgtatca ggtggaaata atgaaaaaat agatgaacga ctgagcaatg
    28921 acctgtcgat aaagtggctc aatgctgttt attattctta tgatatcgta aatggaagtc
    28981 tacaaggaga aattagcaca aagaaacttc cggatgacca aatgaaggaa gcacaattta
    29041 gccaagaaag ctctcgtttg aatttctctt ccagctattc gaaattgcgt gcagttaatc
    29101 ggcgaatcgg tatcaatatt tttgtcacac tatttgtcgg gatgatcgtg attattgcta
    29161 ctggtagtat tttgacagtc cggcaattag cagaagcaga ggaagaaaaa gaaaattatc
    29221 acctattgac taagctgggg attccacaaa gaaggatcag acggatgatc ttcgagcaaa
    29281 acgcacttac cttctttcca cccatgatat tagggatcac tcacgcagta tttgcaatca
    29341 atgtcttcac tcagtatata aaaactgccg attactggtt ggcttatctt gtgagtggct
    29401 tattgatcct tatctatctt ttgctttatg tcattacgag tcagttatat tgtcggatta
    29461 tcgaggaatg
//
LOCUS       DASJUF010000026        27729 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJUF010000026 DASJUF010000000
VERSION     DASJUF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.026"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..288
                     /locus_tag="U4B62_001031"
     CDS             <1..288
                     /locus_tag="U4B62_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141204.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="U4B62_001032"
     CDS             347..787
                     /locus_tag="U4B62_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141205.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="U4B62_001033"
     CDS             799..1467
                     /locus_tag="U4B62_001033"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2141206.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="U4B62_001034"
     CDS             1531..2154
                     /locus_tag="U4B62_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN2141207.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="U4B62_001035"
     CDS             2210..2596
                     /locus_tag="U4B62_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN2141208.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="U4B62_001036"
     CDS             2809..4200
                     /locus_tag="U4B62_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN2141209.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVATQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="U4B62_001037"
     CDS             4811..5179
                     /locus_tag="U4B62_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN2141210.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="U4B62_001038"
     CDS             5359..6300
                     /locus_tag="U4B62_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN2141211.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="U4B62_001039"
     CDS             6369..7100
                     /locus_tag="U4B62_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN2141212.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="U4B62_001040"
     CDS             7244..9424
                     /locus_tag="U4B62_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN2141213.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="U4B62_001041"
     CDS             9444..9926
                     /locus_tag="U4B62_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN2141214.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="U4B62_001042"
     CDS             9942..10703
                     /locus_tag="U4B62_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2141215.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /locus_tag="U4B62_001043"
     CDS             10834..12168
                     /locus_tag="U4B62_001043"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN2141216.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="U4B62_001044"
     CDS             12475..12960
                     /locus_tag="U4B62_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN2141217.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="U4B62_001045"
     CDS             12953..13636
                     /locus_tag="U4B62_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN2141218.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="U4B62_001046"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="U4B62_001046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN2141219.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="U4B62_001047"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="U4B62_001047"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN2141220.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /locus_tag="U4B62_001048"
     CDS             17252..17881
                     /locus_tag="U4B62_001048"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN2141221.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="U4B62_001049"
     CDS             17933..18265
                     /locus_tag="U4B62_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2141222.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="U4B62_001050"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="U4B62_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN2141223.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="U4B62_001051"
     CDS             19091..20464
                     /locus_tag="U4B62_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2141224.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="U4B62_001052"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="U4B62_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN2141225.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="U4B62_001053"
     CDS             21519..21959
                     /locus_tag="U4B62_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN2141226.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22103..22804
                     /locus_tag="U4B62_001054"
     CDS             22103..22804
                     /locus_tag="U4B62_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN2141227.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22801..23922
                     /locus_tag="U4B62_001055"
     CDS             22801..23922
                     /locus_tag="U4B62_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN2141228.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23937..25169
                     /gene="pepT"
                     /locus_tag="U4B62_001056"
     CDS             23937..25169
                     /gene="pepT"
                     /locus_tag="U4B62_001056"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN2141229.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25301..26209
                     /locus_tag="U4B62_001057"
     CDS             25301..26209
                     /locus_tag="U4B62_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN2141230.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26244..26516
                     /locus_tag="U4B62_001058"
     CDS             26244..26516
                     /locus_tag="U4B62_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN2141231.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26527..27573
                     /locus_tag="U4B62_001059"
     CDS             26527..27573
                     /locus_tag="U4B62_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN2141232.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9326 a         4795 c         6037 g         7571 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgctactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
    22081 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
    22141 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
    22201 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
    22261 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    22321 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    22381 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    22441 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    22501 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    22561 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    22621 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    22681 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    22741 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    22801 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    22861 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    22921 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    22981 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    23041 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    23101 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    23161 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    23221 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    23281 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    23341 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    23401 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    23461 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    23521 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    23581 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    23641 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    23701 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    23761 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    23821 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    23881 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    23941 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    24001 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    24061 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    24121 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    24181 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    24241 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    24301 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    24361 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    24421 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    24481 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    24541 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    24601 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    24661 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    24721 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    24781 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    24841 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    24901 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    24961 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    25021 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    25081 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    25141 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    25201 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    25261 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    25321 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    25381 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    25441 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    25501 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    25561 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    25621 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    25681 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    25741 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    25801 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    25861 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    25921 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    25981 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    26041 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    26101 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    26161 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    26221 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc
    26281 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    26341 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    26401 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    26461 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    26521 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    26581 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    26641 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    26701 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    26761 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    26821 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    26881 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    26941 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    27001 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    27061 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    27121 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    27181 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    27241 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    27301 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    27361 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    27421 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    27481 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    27541 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    27601 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    27661 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    27721 actatgtgt
//
LOCUS       DASJUF010000027        26600 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJUF010000027 DASJUF010000000
VERSION     DASJUF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.027"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..424
                     /locus_tag="U4B62_001060"
     CDS             47..424
                     /locus_tag="U4B62_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141233.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            993..2381
                     /gene="ltrA"
                     /locus_tag="U4B62_001061"
     CDS             993..2381
                     /gene="ltrA"
                     /locus_tag="U4B62_001061"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2141234.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(2503..3798)
                     /gene="purB"
                     /locus_tag="U4B62_001062"
     CDS             complement(2503..3798)
                     /gene="purB"
                     /locus_tag="U4B62_001062"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN2141235.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3811..4935)
                     /gene="purK"
                     /locus_tag="U4B62_001063"
     CDS             complement(3811..4935)
                     /gene="purK"
                     /locus_tag="U4B62_001063"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN2141236.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(4928..5416)
                     /gene="purE"
                     /locus_tag="U4B62_001064"
     CDS             complement(4928..5416)
                     /gene="purE"
                     /locus_tag="U4B62_001064"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN2141237.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(5832..7139)
                     /locus_tag="U4B62_001065"
     CDS             complement(5832..7139)
                     /locus_tag="U4B62_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN2141238.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(7142..7723)
                     /locus_tag="U4B62_001066"
     CDS             complement(7142..7723)
                     /locus_tag="U4B62_001066"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN2141239.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(7839..7936)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(8295..9587)
                     /locus_tag="U4B62_001067"
     CDS             complement(8295..9587)
                     /locus_tag="U4B62_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2141240.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(9584..10207)
                     /locus_tag="U4B62_001068"
     CDS             complement(9584..10207)
                     /locus_tag="U4B62_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141241.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(10210..11046)
                     /locus_tag="U4B62_001069"
     CDS             complement(10210..11046)
                     /locus_tag="U4B62_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2141242.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(11063..11950)
                     /locus_tag="U4B62_001070"
     CDS             complement(11063..11950)
                     /locus_tag="U4B62_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2141243.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(11966..13249)
                     /locus_tag="U4B62_001071"
     CDS             complement(11966..13249)
                     /locus_tag="U4B62_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2141244.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(13932..14615)
                     /locus_tag="U4B62_001072"
     CDS             complement(13932..14615)
                     /locus_tag="U4B62_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN2141245.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(14633..15250)
                     /locus_tag="U4B62_001073"
     CDS             complement(14633..15250)
                     /locus_tag="U4B62_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2141246.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(15260..16084)
                     /locus_tag="U4B62_001074"
     CDS             complement(15260..16084)
                     /locus_tag="U4B62_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN2141247.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(16077..16637)
                     /locus_tag="U4B62_001075"
     CDS             complement(16077..16637)
                     /locus_tag="U4B62_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141248.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16811..17767
                     /locus_tag="U4B62_001076"
     CDS             16811..17767
                     /locus_tag="U4B62_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2141249.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(18072..19028)
                     /locus_tag="U4B62_001077"
     CDS             complement(18072..19028)
                     /locus_tag="U4B62_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN2141250.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            19132..19734
                     /locus_tag="U4B62_001078"
     CDS             19132..19734
                     /locus_tag="U4B62_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2141251.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(19822..21321)
                     /locus_tag="U4B62_001079"
     CDS             complement(19822..21321)
                     /locus_tag="U4B62_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN2141252.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(21337..21861)
                     /locus_tag="U4B62_001080"
     CDS             complement(21337..21861)
                     /locus_tag="U4B62_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN2141253.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            22159..22908
                     /locus_tag="U4B62_001081"
     CDS             22159..22908
                     /locus_tag="U4B62_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2141254.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            22899..23684
                     /locus_tag="U4B62_001082"
     CDS             22899..23684
                     /locus_tag="U4B62_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN2141255.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            23653..24369
                     /locus_tag="U4B62_001083"
     CDS             23653..24369
                     /locus_tag="U4B62_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2141256.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(24471..25505)
                     /locus_tag="U4B62_001084"
     CDS             complement(24471..25505)
                     /locus_tag="U4B62_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN2141257.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(25509..26567)
                     /locus_tag="U4B62_001085"
     CDS             complement(25509..26567)
                     /locus_tag="U4B62_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN2141258.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQSMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7993 a         5668 c         4768 g         8171 t
ORIGIN      
        1 gaattaacta aaaatttgga taatgggttc gaggaggcac ttgccgatgg aagatgcaat
       61 aaaacaaggt cagctttttt tatcagaatt aacagctgaa gaacgttcct gtctatataa
      121 tgatttgctg tcgatcaaag acgtttattt atgcaataaa gatactagtt tttatacatt
      181 ccgaaaaatc cgccgagcaa ccaagcgctg tgctgaatgc cgtaaaagga cttttcttta
      241 tcagacagaa cacaaaggcg aactttcaat cagtcagatt catgaacaga agcgtttcaa
      301 aaatcattat cgtatccaga tcttattcaa tggacaagag atggaatacg cttttctcga
      361 aaacttgctg caacatctcc ataaccagca ttttagttat aatagccaag ccatttacgc
      421 ttaattcctc tattttttgt atgtaaagag gcacggtaaa gaggtagccg cttttaaaat
      481 caggttccta ttattgataa gaaaatataa taaactaaaa gaagcagaac cgctaacgga
      541 tctgcttctt ttttggtgtg cgccctgcat gggtgaaaac ttggtggtga aagtccacta
      601 caggcttggc agtaggaact gttagcgaaa gacaaggcgg caatcgtgag gttgtggctg
      661 aaggaagtcg gacgcaaact cctgaactga cgaacagaaa ctagatacaa ggctgaatta
      721 ggtcggataa gtcttcatca caagacaaag tccaatactg cccgaaacct atacagtaaa
      781 tctagcagtt acatgggagg aaagttttca ctcttaccgg gggagatctc atgagggtgt
      841 tgtgggcgtt gaatgaaaac aaaccagcac aacgatcatg gcgacatgat ggtgaatcat
      901 gagaagtcag ccgaggtcat agtagtaacg acactgaccg ttacgaagga ctgaacaata
      961 agaatcttga ataatttagg aggtgtgaga agatgcgacg accgcagaaa acaagaaaac
     1021 ttggctatca tcaaagagct agtgtggaac acgaagggta tgatgaagtg cgcagcactt
     1081 cttgcggtga acagaggaaa caagatggtg tagaaaatct gtttgggaga attctagatc
     1141 ggaataatct caatcaagcg tatcttcaag tagtcagaaa taaaggggcg gctggtgcag
     1201 atgggatgac cgtcgatcaa ctgcttcctt acttgaaaga gcaccgcgag gaattactat
     1261 ccaatttacg ccaagggaac tataaacctc agcctgtttt gcgagtagaa ataccgaaac
     1321 caaacggcgg tgtaagaaaa ctaggaattc ccacggttat tgatcgtatg cttcaacagg
     1381 caatcaatca agtgttacaa ccgctatttg aaccagaatt ctcagataat agttatgggt
     1441 ttcgcccaaa tcgcagcgca catgacgcag tgaagaaagc aaaatcttat tatgaacaag
     1501 gttaccgata tgtggtggac attgatatga aagcctactt tgacaccgta aatcacgata
     1561 agctgatgta tttcattgag cggaaagtaa aggacaaaca agtcctaaaa ctgatccgtc
     1621 aatacttaat gagtggtgta atggaaaatg gactcatcac aacaactgaa gaaggaacgc
     1681 cccaaggagg caatctctct cccctgttaa gtaacatcta cctgaacgaa tttgatcaag
     1741 tgctagaaaa tctaggacat aaatttgttc gatacgccga tgactgcaat atctatgtta
     1801 gaagcaaacg agcaggacag cgtgtgatga agaaatccat tcaatttctc gaagaacaac
     1861 taaagttgac ggtaaatcga gaaaagagtg cggtaggaag tccattgaaa cgaaagtttc
     1921 ttggattctg cattctgcca acaaagaaag gtattaaaat tcgccctcac gcaaaagcaa
     1981 aacagcgtgt gaaaagcaag ctcaaacaac tcactaaaag aaaccgtgga agaagtatcc
     2041 aacttatttt aaaagaaatc aagcaactaa tgactggttg gatcaactac tatggaattg
     2101 gtgagatgaa agaattcatg agggagttaa acggttggtt gaagcgaaga atcagacaat
     2161 atatttggaa acaatggaag aatccaagaa ccaaaagaaa aaatctgatt cgtttaggga
     2221 tcgataaagc gaaagcgtat gaatggtcaa atacaagaaa aggtatttgg tcagtctcca
     2281 aaagtcatat tcttcatcga tcattaacag acaaagaact ggcccgccaa ggatatgagg
     2341 acatctcctt gaagtaccag ttcgtacact caacttattg aaccgccgta tacgggtccg
     2401 tacgtacggt ggtgtgagag gggcggaagg tcagttggct ttcccctcta ctcgattatt
     2461 tttgctattc tattttaatt gaattttgca actcactctt ctttattcca aacctactcg
     2521 tttgaatatc acgtcaacgt ttttcaaatg atgatgataa tcaaaggcat cgtctatttc
     2581 ttctttagat aataccgatg tgatggttgg atcttcttct aacagcgggc ggaactgcac
     2641 ttggttgtcc caagcgtacg cagtttttgg ctgaacaaga tcataagctt cttctcttgt
     2701 cattccctta tcgatcaatt tcagcataac gcgctggcta taaatcaaac caaatgttgc
     2761 agacatattc cgcttcatgt tttcaggaaa gaccgttaga tttttaacga tattgccaaa
     2821 acgatttaac atgtaattca ataaaatcgt ggtatcaggc aagatgattc gttctgctga
     2881 agaatgagaa atatctcttt catgccataa tgtgacattt tcataggcag tgaccatgtg
     2941 tccgcgcacc actctggcta gaccagccat attttctgaa ccgatcggat tccttttgtg
     3001 cggcatagca gaagaacctt tttgaccttt cgcaaaaaat tcttctactt cacgtgtttc
     3061 ggatttttgc aatccgcgta tctctgttgc aaaacgttca atacttgtag caataagcgc
     3121 catcgctgag aaatattcag catgtagatc ccgagggaga acttgagtag agatttcttg
     3181 tggacggatc cccaattgtt tacatacata ctcttcaata aatggcggga tatttgcgaa
     3241 cgttcccact gctcctgaga tttttcctgc ttctactcct tttgccgcat gttcaaatcg
     3301 ttcgatgttc cgtttcattt ctgaatacca tgtggcaagt ttcaatccaa aagtagttgg
     3361 ttcagcatgg acaccgtgtg tccgacccat catcactgta tatttgtgtt cttttgcttt
     3421 ttcgccaatg atttccgtaa aacgtttcaa gtcttcccgt aaaatatcat ttgcttgctt
     3481 gatgagatag ccataagcag tatctacgac atccgtactt gttagaccat aatggaccca
     3541 ttttctttct tctcccaatg tttcagaaac cgctcgtgta aacgcaacga catcatggcg
     3601 tgttaatttt tcgatttcca agatccgatc gatatcaaat gatgcatgct cacgaatttt
     3661 tttcacatct tcttgcggga tttcaccaag ttctgcccaa gcttcatctg ctaaaatctc
     3721 gacttcgagc cacgcacggt atttgttctc ttccgtccag atattcccca tttcagggcg
     3781 tgtataacgt tctaacatgt tttttctcct ctaatcccaa atattcgttt gtttgatttc
     3841 ttccagcgtt tgctggatgt cttctgtcaa gatagtgata tgtcccatct tgcgaccttt
     3901 ttttgcttgt ttttttccat agaaatggaa ttgccagtct ggcttattct caatcaagcg
     3961 atagctttcc agcagttgtt ctcccaaaac attgaccatt actgcattgc ttatcaaacg
     4021 tactttttct cctaatggcc aaccacaaat acctcgaata tgggcatcga actgactcat
     4081 agaacaagct tcaatagaat aatgtccaga attgtgagga cgaggagcta attcattcac
     4141 atatagcccg cctgtttttg ttaaaaacat ttctacccct aaaacacctg aaaggctcac
     4201 tgcttctgca atcacacggg caatacgctc agcctcctcg atcacatcct gatcaatcgc
     4261 ggctggagca atcgtttcat gaagaatatt gttgtggtgg atgttttcca cgaccggaaa
     4321 agtcgtgaaa tctccttgac catttcctgc caccatgatc gaaatctctt tttcaaacgg
     4381 gatccatgct tctaaaacac aggttccttc tcttaaaagg tccatggcag gagctagatc
     4441 cgcccgactt ttcaaaacat actgcccctt gccatcatag ccgcctcgag tggttttcaa
     4501 aacacaagga tagccgatcc cgtcaatcgc atcttggata tccgtcggac tgacgatcgt
     4561 tgcataagga gcaatcacga tgttattcgt ttccaaaaaa gatttttcta acaggcggtc
     4621 ttgcgtaatc gcaagcaagt ctgtcccttg aggaataaat gacatgggta aaatcgcatt
     4681 taacgcctct acactgacgt tttcgaattc ataggtaatg acatctgttc ttctggcaag
     4741 ctctttcagc gcaaaggtgt catcatagac actttcaatg tgccaatctg caacttgtgc
     4801 ggctggacaa tcgctcacag gatccagcac acctacttta aagcccattt cttttgcact
     4861 aatcgccatc atacgaccta attgaccgcc accgacgatc ccgatcgttg aaccaggcaa
     4921 caaaggttta accaagttga tcactacttt ctataacagt tttagccaac attgaacgtc
     4981 tttcagccaa tttagcctcc aattctaaat catacatgga aagcatctgt atcgcaagta
     5041 gtccagcatt aattgctccc gcttttccga ttgctgtagt agctacaggt actcctccag
     5101 gcatttggac aatcgaaaga agcgaatcca aaccatttaa tgttcgtgat tggaccggta
     5161 caccgataac cggcaaagtt gtttttgatg cgaccatacc cggcagatgc gcggctcccc
     5221 ctgctcctgc aataatgatt ttgattcctt tttttcgagc gttttgtgca tattcaaaca
     5281 tcaaatctgg ggttcgatga gcagaaacga cctttttttc ataagacaca ccgaattcat
     5341 ccaatatttg acatgcttct ttcattgttt cccaatctga agtactcccc ataatcacag
     5401 agatttttgc tgtcattgtt tttcctccca gtttttctac attcatattt taacaatcac
     5461 tttttttgat gtcaaagaaa tttcgaatgt taacaaagaa aaacatttta ttgttcgtgt
     5521 ttattaaaga taaaaaaaag gaatgtgaca aaagttaggt cacattcctt tttatggata
     5581 aacggtgttc tcctgccaac tcctcggtca caaggaacaa tattcaacaa acatgagaag
     5641 cttttttttg aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttcc
     5701 ttcaacggtg ttcaatcagc tgaccctcgg tattagctca aacaaacggc aaaatacgag
     5761 gaactatttt tccgtttgtt tctctaacaa tcaggtcaaa acgctgattt cacaaccttt
     5821 cttttgctct attacttctt gaaggcttct tccaatattt cttcatcttt tttcaatcca
     5881 ttaaagatca aattcaatat gatagccgta atgctactca ttacgatgcc atttccagtg
     5941 aacatttgca atgtttgcgg catttgactg aataatgtcg gcgtaacatt gaaacccagc
     6001 ccaaatccaa cagaaacagc aacaatcaat agatttttat cattgctgaa atcaacttcc
     6061 aacagcatac ggattccttg gactgccacc atcccgaaca tgactaacat tcctccacca
     6121 agtaccggct cagggatgat ttgtgctaac gcaccgattt tagggaataa tcctaaaaca
     6181 atcaagaaga atgcagagaa atagatgggt tttcttgttt tgattcccga aagttgaacc
     6241 aatccgacat tttgagaaaa gccagtataa gggaatgtat tgaaaatacc gcctaagata
     6301 acggcaagtc cttctgcacg atatcccttt ttcaattctt cttctccaac atttttccca
     6361 gtaatgtcac caagtgcaaa atatacacct gttgattcta ccatgctgac catggaaatg
     6421 atgatcatta gtaaaatcga ccatacgtcg aaagttggtt taccaaaata aaagggttgt
     6481 ggaacatgga acaacggcgc atctccaatc gcgctcagat caatcattcc catgaaagca
     6541 gctaagatgc ttcctccgac taaaccaatc aataccgcga ttgaacgaat gaaccctttt
     6601 gccagcattt gcacgcctac aattaggaga atcgtcacaa atgcaagtag cagatttgtt
     6661 ttgtcaccaa aatcagctgc tgttgcatta cctccaccca ttttctcgat tgctacagga
     6721 atcaaagtca aaccaataac ggtgataacc gttcctgtta ctataggagg gaatagtttt
     6781 ttaattttag aaaagacacc agaaactaac acaacaaaga tcccagaagc gataatagat
     6841 ccgtagatag ctcctactcc ttcattggtt ccgatcaaga tcagtggagc tactgcttgg
     6901 atcgcacatc ctaaaacgac aggcaagcca attccgaaaa atcggttaac tgtcaattgc
     6961 aataatgtcg ctaatccaca cataaagata tcaattgaaa tcaaataagt catctgttct
     7021 tgattaaagc ctagtcctgt accgatcaaa agaggcaccg ctactgctcc tgcatacatc
     7081 gctagcagat gctgcagccc taaaaccgca gcttttccat tttgtgtttc tttttccacg
     7141 attatgcgtc ctcctcaaga aactcaactt gtccattcgt caaggaagca atacgcgcaa
     7201 gagaaacgac tctcaatccc atttcttcaa gtaactgacg tccatcttgg aaagactttt
     7261 cgatgacgat cccgattcct tctacttgcg ctccagcttg ctggcataat tcgatcaatc
     7321 cttttgccgc ttgtccgttt gccaagaaat cgtcgataat caatactttg tcttctgaag
     7381 ataaaaattt acgagaaata gaaatcgtac ttgtcacttg tttcgtaaaa gaataaacag
     7441 aagctgtcaa taattcttca tccatcgtca aactttttgc ttttctagcg aagatcattg
     7501 gaacatctaa ttctcttgca gcaaataaag ctggtgcgat cccagaagct tcaatggtca
     7561 caactttcgt gattccttgt tctttaaata cttctgcaaa ccgttttccc atttgttcca
     7621 tcaatacagg gtctacttgg tgagtgacga agctgtcgac ttttaatacg ccctcgccta
     7681 atatacgtcc gtctttttga atacgttcaa ctaattcttt cactttattt gctcctttat
     7741 catttattaa taccgaaata accgcttttg tgcaaaaacc tactatgttt ctgttaaaga
     7801 aaaacaccct ccactttatc ataagaaaag aagggtgaaa tagccaatct tttacttata
     7861 gtccagcatt tacggtactg gctagaaact gtcatccata ttatgacgat atataagtag
     7921 gtatgtaaaa ctaataatag tgtaaattca ttttcttgca actgttttgc acgtgaaggc
     7981 agtttgccat atttgaataa aaaatgttgt ttcaaatatt gaaaacagtc taacactact
     8041 ttctgaaact gacaacgtgg tttacttatt tttttgtaaa atctaatgat ttttaatgca
     8101 ttattccttt ttgttcgggt ttaaatagtt atttttattt tctcctatct aaaaaagcag
     8161 cccacataaa aagatggcat gacaaaaagc atgtcatagc ccctttttcc gaataaatag
     8221 tgttcaaaag acagcctatc ggcaataagg caagatttta taaactacgg gaagtaattt
     8281 ccgtaaatct attcttattc ttttaagctg acaagctcgt caatcactac tgcttttcct
     8341 gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg acaaaatacc atctacgtga
     8401 gttgctgccc gattccacgg atcttcttct ttttcttcac caaatagatc atctaataaa
     8461 cgcggatctc ctccgccatg tccgcccgct tcttgttcga cgattacctc atatggctca
     8521 ccgaacatcg gatagacatg gatggactga agaacggctg ctccttcatc ctttttatct
     8581 ccacctgcat tgatgtaaga cttttcgatc actttcattt cgatccgtcc tttgctgcca
     8641 ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt ttagcgaata cgtcaaaatc
     8701 gcaccagttt catatttgac cgttacgcct agtgtatctt caatagagat atcatcggaa
     8761 aatacactgc ggtcacgaat atatccactg tctttttctg cttctaaata catttctcgc
     8821 aattcctcat tggctgacag gtctatagca aatggatcag ctttagctag ttcgcttcca
     8881 tagcatcgac tgtaaaatat ttctactccg cgtttctccg cattatgcat cccataaaag
     8941 tttaatccac cgaatgcaaa taccttttgg ggacggctgt tcaaccaaaa attcactaga
     9001 tcaaaatgat gggtagattt atgaacaaga agcccgccgc tattgtgctt atttcgatgc
     9061 catcttctga aataatctgc cccgtgttgc gtatttaaca gccattcgaa gtggatagaa
     9121 aacacatcac caatcacatc actttctatc agttccttta tcttcgtgtg atacggtgca
     9181 taacgataat taaaagtgac tcgcaggctt cttcccgtct gtttttggac cgttattatt
     9241 tcttgagctt tttcctcatc gatagtcaga ggtttttcgc aaataacatc atatcctttt
     9301 ttcattgcac gaatgatata atcatgatgc gttcgatcta ctgacgtaat aatgacttca
     9361 gccggccgta cgtcatccag tagttgctca aattgtgatg gcatacatgt ctttactttt
     9421 ttctgtccat acttgttcac aagtatccga tttgcataat ccatccttgt ttgagataca
     9481 tcacaaaaac cgactagttc acaagtttga tgatacgtag aggcaattgc ctcataaaaa
     9541 aaacgcgctc tcccaccaag tccgacttgt acatattttt tcttcattta aagtcattct
     9601 ccttttcttt taagcaaaat actgtgacaa atggcagccc tggatacagt aatgcagtga
     9661 gtaaaaaatc atgctgtata caccagaaca gtccatacaa aaggcataaa taccacagaa
     9721 accccttgac gttttttgac aacagccaga tattcctttt gatcaaataa acaagactat
     9781 ctgctttatc ttctttgcag atatcaatta gcgtactaaa aaaactatgg gatacaaaaa
     9841 gcaacagaca caatagaaaa ggaatcagat accaagctcc aatctttccc actaataaat
     9901 accaatcaac tgcaaaaata atgacaaaca gctgaaacaa cgtaatgatg aaacacatcc
     9961 gtttgatttt tcctttcctc caacatacct ttttttctgc caagtaatgg acagatagat
    10021 aataagaagg atacatcatc aaaaaataaa tcagatagac aacacgagca gacagcacta
    10081 atcctaaagt gaacaaacat cctagaacta aagcgaaaag agcattactg ataagactgg
    10141 ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag aaacatctct tctattggct
    10201 ttttcatatt cagcccttca gaccgcttgt actgatcccc tggacaagat atttattgaa
    10261 gaataagaaa atcagaaaaa ccggtagcag cgacaacgta gacatcgcaa acatactcgc
    10321 gtaagtcgta ctagatccag cgtctgcaaa taatttgatg gcaatcgata ccgtgtaaga
    10381 tctcggcttg tttaaataaa tcaatggtcc catgaagtcg tcccatttcc aataaaactg
    10441 gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga aaaataattt tggtaaagac
    10501 actatacttg ctgcatccgt caataatcgc tgattcatct aactctttgg ggatgctgac
    10561 catgaattgc atgatctggt aaatgaaaaa tgcttgtcca aaaaaatgtg gtaaaatcag
    10621 cggcacatac gtcccaacca aattcagccg attgtaaata ataaactgcg gaatcaaaat
    10681 cacttgtgca ggaatcatca tcgttactaa catgacgacg aaccagaatc tttttccttt
    10741 gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag ctcagaactg tccctatcgt
    10801 cgaaactgca gagatgacaa acgaattcct aaagaaagta aaaaatgaaa ttccagcaaa
    10861 ccccgcccat ccttttgcgt aattttccca ttgaaaagtc tctggaatca aattcagcga
    10921 accactcata atctcttgat ccgttttaaa agaacctgcg atcatccata agaccggata
    10981 aatcatgact agacctaata gcaaaataaa accgtattga aaccatttcg ttactgtatt
    11041 attcatttct tcctcctctc attcacacaa cgacccattt ttttgatgat ttgaaaatca
    11101 gaaaggtgac agctcccata atcaacagca tgatccacga aatagcacta gcatacccca
    11161 tagaattaaa agaaaaagct tggttataca cataaagagc ataaaagttc gtggcatcat
    11221 tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt cataaacgcc gcgattgtct
    11281 gcataaccaa gttatacaaa atgatcggcg aaagaagagg taacgtgatc ttgtaaaacg
    11341 tctgccattt gttcgctcca tcgatctctg ccgcttcata atacgagcct gggatctctt
    11401 tgatacctgc agcaaagatg atcattgccg aaccgaattg ccaagcagac atcaaaacaa
    11461 gagggatcat gaccagtttt tgatcgctaa accaggaaag cgcaggtgcg cctaatcgtt
    11521 ccaggaaaat attcactaac ccgtttttag aaaacaactc ccgccaaact agagcgatag
    11581 aaatactgcc accgatcaaa ctaggcacat aatacaaaga acgaaagaaa gtcgttcctt
    11641 tgatattcct cgttaaaaga taagcaatca atagaccaaa gatcagtttc acaggtacag
    11701 atgcaaatac atagagaaaa gtgactcgga tggagttcaa aaaacgggca tcactcagca
    11761 tctttatata attatccaag ccgatccatg agactggatt tcccaaacga taatcggtga
    11821 acgaataata aagtgacaag atcatcggta tcactgttat caatagaaat cctaggatga
    11881 atggagaagc aaatacgtaa ccgaccacat tgtcttgatc caagaaccgt tttagttttt
    11941 cttttttcat gccttcctcc tgctgctatc ctagaatgtt tgacgcaaac tcataaagac
    12001 tctctgctgc ttcttctgct gttacttctt cataaatgac tttttcaact aaaagattgt
    12061 attggtcttg aatttccgga ttcaaagcac tatcaaagac attcacttcg ccgttgtcca
    12121 tatcggaaat catttctaaa taatcaaatg tcgcttgcaa agttgcatca tctgcacgag
    12181 cgagctgttc tcgaatattc gacatgattg gtacaccacg ttctcctttt aaaatttcat
    12241 tggcttcttc actgttttga aagaaatcaa taaacttagc agcttcttct ttatgggctg
    12301 agctttttgc catactcagc atttgcgaag aacgtaaagg gataccagat tctccatctg
    12361 cttttctgcg aggtggatta atcaactgaa tctccatatc ttcaggagcc cctgacaata
    12421 tttctgtcaa ctggttactt gatagaaaca ccattgccgc ttctctatcc ctaaccgcct
    12481 gaaaagacag atccgtcgta cttgcagctc caccacctgg ttctggatgg gcccctttgt
    12541 ctaccagatt tttacgcagc tggaaaaaat ctgttagata gtctggattt tcaaatccca
    12601 agctgttaga tagatcttcc cgattataaa aattcaatcc tttctcaact tgtggtattt
    12661 gaataataca gccagctata aaatcttcga atcttgaaat agcatagatt cctaattctt
    12721 cagtgatttg atagctgatt tgttcaaatt catcccatgt ccaatttgaa gctggttcag
    12781 ggatatttaa ttgttcaaat atatttttat tgtaagcaat cgcaaacgaa ttcaccccgt
    12841 tagacagtcc aactaattgc ccgtcgtaag tcgtcgttgc taaataggat tcatctgcat
    12901 tagaaatatc aattatccct tgctccacat attcatctaa tggttcaatc tgattgatgt
    12961 attgaggaaa ttgattgttc agttggaaaa tgtcccagat atcatctgat gctgctagtg
    13021 tacctaattt tgtgtaatat ccttcataat cataaaattc ataatcgatc gtcacatggg
    13081 gatttttttc ttcataaagc tcgattactt ttactgttgc atcgtgtcgg acttgcgaac
    13141 cccaccaagc catgttcagc gtgatttttt tgtcatcttc ttgcccacaa gccgtcaaga
    13201 agaataacat aacacttaaa gctaagatga ttctcattct tttcttcatt ctctgcacct
    13261 ctacttccat tgttattttt caatatactg tatttttata cagaacatat ttattaaacg
    13321 atctttttca aggggaataa aaaccaagac ttgccttatc gttctgccta ggatgaacaa
    13381 ctcaatactt tcaagaaaaa ttagatagtg ctgattcttc tttcatcgct aaaaacactt
    13441 cttgcttcaa tgatgggagg ataattacgc aaagaaaaaa ggatatcaga caactgccca
    13501 atatccttga catagctaaa taagcatgct tcttcgtgtg cgctcttccc taaaaaacag
    13561 aaaaataact agtaattctg tatgaaatta ggtacaatat acacgtcgat gcacaagcat
    13621 tgactatggc aacttgattg gtcagttccc gctgactttt caagccccga caatttagct
    13681 gctactaaat tgtcgggtgc cttttttagt ttttattcgt ttttcattct atagaaagcg
    13741 cttcaataaa tcaagacatt tttttgttag aatgctaata ataacaaaag gttttaactc
    13801 ttcttatgta cattacaggt aaaaaaacaa aaaaatttct cttttttttg aattctgaac
    13861 aaatagtact ggagtacaaa agacgaggaa gagccactat ctgctcttcc tcgtcttttg
    13921 attctcacct gttattcatg attctttttc tcagtttccc gatatttttc cattttacga
    13981 ctaaaaagct ctccgctact tggtggcgta tatgtgatgg catttgctcc tgcttgaatg
    14041 gtctcagcaa tactttcttc cgttggacca cctgtcgcaa tgataggaag ttcaggatat
    14101 tttttacgaa aatggcgtac tgcttctgca gtttctttac ctgcactgat attgataatc
    14161 gaaataccag ctgctaactt ttcatctatt tctgtatatt tcgacgttac cgtactgata
    14221 actggaatat caactactcg acaaacttct tttactgtct ctaaaggcgt aggaccgttc
    14281 aaaacgaccc ctaacgaacc ttgcgcttct gcgaacatgc tcatgtagga agaacgagca
    14341 ccttgtgtca aaccaccacc tacaccggag aaaactggaa tgtcagctgc ctcaataatg
    14401 cttttagtaa tcgctggatg aggtgtaaaa ggatacacag caattactgc attcgcattt
    14461 gtattacgta tgatcgcaat atccgtagta aagatgatcg aacggatcaa ttttccaaat
    14521 atcttgatcc cgcttgcttc taaaatgatt tccggtactc ttacgatatc ttttctcaat
    14581 tccgtcatga tttctggcgt aaattgttct gccatcagcc acttctcctt tgctatctcc
    14641 ggtcatactg attcaattga tacgtctcga tgacctggat gattttttgc gcataaccgg
    14701 ggtctgtcgc ataacctgca tcttgcaatg cttgtgcggc ttcctgatag ttggtggccg
    14761 taatcacttt ttcatacagc gcaggattcc aatctactcc ttggacgaac agttgtgtat
    14821 ggtcatccat ggactgttcc catgaatcat acactttgaa gtctccttgg atagttatcc
    14881 attcttcgtt aacaaattcc tttgtttcca ggcttacttt cttctgatcc ccataagcct
    14941 tgatcccgaa aagattattg tatttgctag ccaaggtact ttttccccaa tttgattcta
    15001 atatagcttg tcctaaaatg atactaggaa gcactccata accatcttgt agttcttggg
    15061 catgcggaac aagccgttca ataaattgct gatgggattg cgcttcattt tcttgctgat
    15121 tatcagtaaa aggagaagac agattcttca aggaaaaaac aaaggctaag agtatcactg
    15181 ctacaccggc aaccactgct ggccaacgga tattcttgcg cttttgcttt ttccactttt
    15241 ttttcgccat gatcaattcc tacttttcat ttaattcatt cagatattct tctagcgtca
    15301 attggtgttt ggtgatatac tcagcgtttt ctttaccgac ataacgtaag tgccaaggtt
    15361 catatgtgat cttagttata tcttcgtgtc cttttggata acgaacgata aatccatact
    15421 gcgcagcatt atctgcgatc catttagctc caggctgatc tccgtaactt gcatccaata
    15481 cagtagaagg ataactgttg taccagtttt gatcgacgac atcgactgcc agacctgtgt
    15541 ggtgttcgct gtatcctggc tccgtaatcg tttctttagt aatctttgtc gcctcctctt
    15601 ctgatacacc ttgacgactc atcacatctt ggacattttg ttcgaaaact tgttgttggg
    15661 atgatacaga ccggtaagct gagaccatca ctaatggaaa tccagctgct tcagcagcct
    15721 tggcaaatgc ttcatattct ggagcaatgc gtttgtcgat caagtatccg ttacttaatg
    15781 atgccaattg agtagactca tcaatttctt tttctaattt gtgatctgga ccaaccaaaa
    15841 ctagcttcca atcgtctgtt ttaacatctg gcaattgttt ggacgtacga tcttttgttt
    15901 ctttgtcctt taccttgctg ctggttgttt gactgttctt ttctttacta gcagttgttt
    15961 tggtgcttgt tcgttcgact gcttgattgc cggcttgaac atgcgaatca tcatcgctaa
    16021 aaataacata gcccattgct gcgatcataa tcacaactgc tccaaatcct aaaaatttat
    16081 tcacttgaaa aatcctttcg ttgttcttca cgttccccat tcgcctcatg aacgtaagtt
    16141 tcttccagat tttcattcac ccaatcgaga agttcgattt tttctccttc gatctgtttt
    16201 tgaacatctg ctggcagacg aaaattcaaa atatcatcat agccccattc atcatattga
    16261 atacttactt tcaagtcaag gtatctacca gcctgctgat tatttttcaa agggatcagc
    16321 tcgacttttg ctgctttgct gctaagccat ttttgcgcga tgcgtgtttt gatttgcttg
    16381 tactctgcaa cggcaacgtt tcgtttttcg ggaaaaataa tcgtttgacg caaaatcttt
    16441 tgcgttagca tccattcata atcagtgaaa agatttttta ctttctgcat cgcttcagat
    16501 ggcagatttt tctcgccatc tttccatttt tgccattgtt ctggactgat cagcaagtat
    16561 tcatctaaaa aatttttttc agtttcaaac tgttctaata atacactcat aataagatca
    16621 atatatactt gatgcacgtt tatcccctcc ttttaactat tctacttaag ttttgatcaa
    16681 aaagcacctc ttaaatcgct gctttcattt tttttaatgt ttcaagtcct ctcctcttta
    16741 ttcttcgtga tttttgttat aataatagac agacaagaaa ctcaatccaa ctatgaagga
    16801 gcgttttcag atgaaaaaat atcaatgggg aatcgttggt cttggtacca tcgcacatga
    16861 gtttgccgaa agcttcaatc aagagacgag cgaactaaca gcagttgctt ccagaacttc
    16921 agaaaaagcg gaaaacttcg ctcaccgtta caatattcca aaagcttacg gaagctatca
    16981 agaaatgtta gacgatgctg aaatcgacat cgtgtacatt gctgtaccaa atagacagca
    17041 tatcgaccat atcttggcag cattggaagc aggcaaacac gttctatgtg aaaaagccat
    17101 cacgatgaat aaaaaagaac tggctgacgc tatgcgctta gcagaagaaa agaatttgat
    17161 cttagcagaa gcgatgacaa tcttcaacat gccactttat cagcagttac gctcaataat
    17221 ggatacagga aaactggggg ctttaaaaat gatccaagca cctttcggca gttacaaaga
    17281 accagaccct aaaaaccggt tcttcaatcc agaattagct ggaggagctc tgttggatat
    17341 cggtacgtat gctgtatctt tcgctcgttt ctttttgagc tctcaacctg aagtagttgc
    17401 ctctacaatg gttccttttg aaacaggcgt ggacgaacaa tccgtcacta tcttgcgtaa
    17461 caaagaaaat gaacttgctg ctgtcagctt gacttttcag gcaaaaatgc caaaagtcgg
    17521 tatcgttgcc tttgagaacg gttatgtgac gattgctgat tacccgcgtg ctgatcgtgc
    17581 ggagatctta tttacagatg gtacaaagga attcatcgaa agcgggaata catctgaagc
    17641 aatgaattac gaaataaaga atatggtaca catgattgaa ggtgacttgc ccaatcgctc
    17701 tctcttcttg acaaaagatg tcatcgaaat cttggatcaa atgcaagttc tatggaaaaa
    17761 tatgtaattt taaaaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    17821 caaaaatagc tcctcctatt tttcgtttgg ctggacttat taccgcagag gaaagctttt
    17881 gaacaccgtt taagaaagag gttgtgagcc taccgttcag ctccgagtca caaggagcaa
    17941 tttcaaaaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttgaca
    18001 ggagaacacc gtttaaagaa gaggttgtga aatcagcttt ttgacctgaa tattagctga
    18061 attcacaacc tctatttttg gattttttgt tgggagaatt ctgccttgat ttcaataaaa
    18121 cttttaaaag actgcaaaaa ctggaacaat tctgctcgat cctcgaattc agggcgacta
    18181 gtcggcagag gcatttgccg atacaataca taaacttctt caatacgtgt taaaatttgt
    18241 ctgccatcat ttgactctga aaatgtttct gcagtataaa tcaataaacc atagatatgt
    18301 tttccataat aagctggttg ctggattctc tctagatttt ccagcatgtc tttaatcaca
    18361 ttggcctgtg ctcttctcat agagaagtaa gtttcgtagt atagcggctg tctgagccag
    18421 tagttttctt gatgttctct ggcatcttct tggctttcgc gaatgaatgt cagtaaatgt
    18481 tcgcatttga tctgcacatc ttctttattt tctgataaaa gaaattctgc catttgtctc
    18541 aaaatatttc gaaattcttt ttcgatctgt atttggtttt ccttcagctg cttcgtacga
    18601 tctggcatgt aaacatttgc aagcaaagca aatactaatc caatcgccat taatcccgct
    18661 tcattgccga tcagctgcca agacatgttt tcctccacta aataatgagt gaccaaaacc
    18721 gaatttacta ctatcccttc ggttaatttg aagcgagagg caagcgggat gaacaggaat
    18781 aaaaaaagac tgaagccaaa aatagtatac ccaacagcaa aaaaactgaa cagtgctgtt
    18841 gccgtagcta atataaaggc tgcgattcga ttcatgcccg tacgcaatgt agagcgcttg
    18901 gtatttccta cacttaataa tgcaatgatc cctgcagctg gccaatacaa cagcgataag
    18961 ctacccgctg tgaccatcga aaagaagaca ctgatcactg ttttgactgt tcgcattcct
    19021 atacgcattt acttccccct tttttatttt aattatactt gaataatgaa aaactagcta
    19081 tcagacaaaa gtttaggtat gatagtaaaa agaccaaaaa ggagaatctc tatgcaattt
    19141 ggccgttttc gactaggtat gcgtacgatc aaaagtgcct tagctgtttt tttatgtatc
    19201 ttgttttttc atttgactca acgaggaatc ccgatgattg ctgcattatc tgctgttttt
    19261 tctttgcgtc aggacttgac tacaaccgtt tcttttggaa agtctaggat catcggcaat
    19321 acattaggtg gtacattagc tatcatttat ttttttgtca aaagctattt tcacaatgat
    19381 tttttggtag aattgatctt gcttcctatt ctagttatta ttgtgatcgt cgtgtcagat
    19441 ggtattaaca ataattccgg tatcatttcc gcaatagcta cgttgttgct gattgcgttg
    19501 agcatccctc aaggcgaatc cgctttgtat gctatccaac gtgtactaga tacttttata
    19561 gggacattca ttgcaatcgg tatcaatttc tttcttcgtc ctcctgaaac agaaaagaag
    19621 gaagaactat cagaagattt ggtcgaactc caaaagaaag aacgattgct aagaacaaat
    19681 ttacaagaga tagaagctaa gatccaaaaa caaaagaaaa atagcgagga ctaattttct
    19741 tttgcaataa aataaaaaaa tggagagaag aactttaaaa agtcttcctc catttttgat
    19801 tgaacggtga agtgtactac tttattcttc cttcatcgag ttttgttctg cctcttttgg
    19861 cgcttcatac atatttactt ggctagtagt cccacgtgtc gacaaattct ctacgatttc
    19921 cttcaagtca agtcctgttg tcgcttttag tgtttcttga gtagaagaaa gcaaatttgt
    19981 ggcataattt gtcactcggt tcgctccact ggtttctcct gaactgcttc ctgtatcgac
    20041 aacagagatt ttttcgatat ttcctagcgg ctgtgctgct tcgcgcatca attgcggtag
    20101 catatcgata accatactca agacagctgc ttcgccatat tccttgaagg cattggcgat
    20161 cttttgtttt gcttcggctt ctgcttttcc ttttgctagt gctgcttcgg cttctgcttg
    20221 tccagctaaa cgtgtcttat tggcttccgc ttctgccagt gcttccacac ggaatcgttc
    20281 tgcttcggct tcagtcactt ctttgacttt ttgagcttgt gcttcttgtt ctttagcgta
    20341 ccgatcggca tctgcttttt tcttcacttc cgaatcatat tgcttttcac gacgtgtgat
    20401 ttctttttct tccagttcga tttgtttttg acgttctacg attttaatct gcatttcctg
    20461 ttcgattact tgttgttgtg ctcgggcact ttctaaattg taggcttggt ctgcttttgc
    20521 tttagcaaca tcttgttctt gtttgtaggt tgctaatttc agttcttttt cttttaatga
    20581 ttctgcgatc tctgtttgtc gctgtaattc agctgcttgt gattcttttt ctgcttgagc
    20641 ttttttgata cgcgtttctt ttagagcttc cgcttctgct atatctgcat ctcttttgac
    20701 ttgggcgatt cttggtttcc ctaaagagtc aagataaccg tttttgtctc tgacctcttt
    20761 gatagtgaaa gaaacgatga tcaaccccat ttttgcaagg tctacagaag cgacttcttg
    20821 tacgctttga ctgaacttat cgcgattttg atagatttct tctacagtca tagaaccaag
    20881 aatcgagcgc aagtgtcctt ctaatacttc tcttgcttca ttttccagtt cttctcttgt
    20941 cttgcctaaa aactgttctg ctgctgtagc aatctcttct acagatgaac cgattttgat
    21001 gatcgaagta ccatcacaca tcaccggtac gccttgttcc gtatacactt caggggtgga
    21061 gacgtccaat ttactcgata atagactgat ccgattcgaa cgttgaaaaa ctggcaagac
    21121 gaatgcgcca cctccacgca caattttgat tttgttattg ctttcatcaa tgtgcacatt
    21181 tttgcttcct aaataacttc cactgatgat caacgcttca tccggttttg ctgtctggta
    21241 cttcgctaca aagacgatca gcaacatcag taaaatcaaa gctgcgattg cgatcgcgat
    21301 aaaaacaact ggtatttcca tttaacacac gctcctttaa gcttttatca tgctttcata
    21361 aggcatgaca taacagattc tttccttgat ctcaatgatc aatactttcg ttccccgctc
    21421 agctgtctgt tcttgggggc tgtaaaaaca agctggacga ctaatggaac cggtaacact
    21481 tttcaactga atttctccca tacctcgtac agggatcggt gtgatcacaa cagctacttg
    21541 tccttccaga gttttttcag aagacgacat cgttgattcg gcatgtttca tcggcatcaa
    21601 tatatagaaa ttaagcaaaa ataccaacac tatcgatatg cctccactag caataaataa
    21661 aaataaagat gattgatctg ttaaacgctc acctagaaaa ccaaataagc ttgtgaaagt
    21721 taaccaaggg ataataagca tcggatcaag cgggccatca aaatcgaata catctccaaa
    21781 aataatcaat aggactgcca atcctgcaca aatcagcaat gtataccaat aaatcgtctc
    21841 aagtgcgatt ccccctatca tatacacgcc tccttccatt tctacgtcta tcataccaat
    21901 gtttgaaaat tctgtatagc ttcttttacc gactcagtgg tgttttttat aggaaggttg
    21961 tgtttatcgt tgacaatttg ttaaattttt gttttcatgt taaaattttc acaataaatt
    22021 ctttcgaggt agcctatgtt tccttttttt aagaaaaaac cattaaagct ccagaacacg
    22081 atcatccttg tcgtttgttt ctccacattc gtttctttga ttgtttctgc catattgatc
    22141 aggaatttta ttctttcgat gaaccgatct agtgtgtcag ataaaatctc tagcattgct
    22201 aaaatcgttg ccaatgataa actcatccaa aaaaagctgc agactggtga cccgacacat
    22261 gcgattcaag gaataacaaa tgaaatcatg attgcatcga atttagattt tatcgttgtc
    22321 atggaccctg aaggcattcg atattctcac gcagacccaa gttacatcgg tcttcctttc
    22381 tcaagtcatg aagatgtgaa aaaagtgatt ggaggacaag aatttttctc tgaaaaagaa
    22441 ggaatgttag ggttcggctt ccgctatttc gtccctattt attatgatca agagattatt
    22501 ggtgcagttt gtgttggctt aacaaacaac acgattgaca atcaagtcaa agatggacaa
    22561 aaaatgattg ttttttcatt gattattgga cttagcgtag gcatcatagg cgccgttttt
    22621 cttgcccaaa aaatgaaaaa agtactgctt ggacttgaac cacaagaaat agcagaacag
    22681 ttaagtgaaa aaacgattat cgaaaatgaa gtagatgaag gaatcttagc catctcttct
    22741 gataaaacta tcctcttgat caatacacct gcaaaaaaca ttttctacca aacagagcag
    22801 ctattggatt tcgaagtagg aaaaaaatca gtgataatct atatgacgtt ttgtttaaag
    22861 agacgattga gcatcaaacg accgttcgga ctcaatccat gtatttaaat gggatagaaa
    22921 ctatttctag cgttacacct ctttttagca atgaccgttt tttaggagca gtcgcaactt
    22981 tacgaaatcg atcagaagta atgaaattaa tggaggagct tggggggacg aaacaatata
    23041 taaactccat gagagctcaa acccatgaat tcatgaataa acttcatgtc atttctggtt
    23101 tgattgattt gaagaagtat gaagaagtca cccattttat ccagcagcta aatcaaaatt
    23161 ataaaaaaga gattggccaa gtctcaagtc tgatcaaagt acctgcaatt gccggttttt
    23221 tattaggcaa aataaacgaa gccaaagaac aatctgttga aatgctgtta gaaagcgatt
    23281 cttttatccc taaaatggag atggatgact ccatccattc acttttgcaa gtgttaggga
    23341 acttacttga taatgcaaaa gaagccgtgc tacaaaaaca tgaaagaaac ggacaggtcc
    23401 ggattcattt aaactatgac acagaagctg aagtattcat cgtgaccgtc tgcgatgacg
    23461 gtgtcgggat atcagaagaa atctcagaaa aaatatttac gctaggtttt tcgacgaagg
    23521 gaatcaatcg tgggtttgga ttgaatctca tccattcaat cgtcaaacgc cacggtggat
    23581 ttttagaatt ttccaaacaa acaaatggcg gtacaatcgt ctatctagaa ttaccaaaaa
    23641 aggaagatca aaatgatgac aaatgtcctg ataatcgaag atgatccaat ggttgcagaa
    23701 ttgaatcgta cttttgtgga aagcatccct aatatgaacg taatcgaaag tttcagaaat
    23761 gtagaagaag gaaaaaaata tttcagacaa aaaaacgcag atctgctgct gcttgatgta
    23821 tacatgcctg gcgaaagtgg tatccatttt ttatcttggc ttagacagca aggaaatgat
    23881 atcccagtga ttttgatcac tgctgcagat gatatcgaaa ccataaagaa tgtgatccgc
    23941 tacggtgcct tagattattt gatcaaaccg ttcactttgc aacgttttca gctggcgatc
    24001 gaaaagtatc ttgctttctc tcagacaatc caaacgactg aaaaagccga tcagaaattg
    24061 atcgatcaag tattcaacca caccactccc attcacgaaa taccatcatt catcgatttg
    24121 cctaaaggtc tgtcaaaact gactttaaaa aaaataagtg catttattct aaaagaagat
    24181 actatttttt caacaaaaga actggctcaa aaaagctcac tgtcacgtat atccgtcaag
    24241 aaatatttga attttttagt agaacaagat atattgaacg aaagaatcaa atatttggaa
    24301 atcggccggc cgctaacctg ttatgaattg acaaagaatg ggcggaaaaa gctcacagat
    24361 tactgttgag tcatcgaatt atcccataca aaatagtcca agtatacaga agtaacaact
    24421 gtttacttgg actattttta aacattttat tgacgaatat ccatgttttc ttatcttaat
    24481 tgacgggctt taggaagaga tacctcattt ttcgtttttc cagtttctat tatttcacga
    24541 aagttctcga aacttgtcga aatcatattt gataatgcct catctgtatt tgaacctaca
    24601 tgcggggtta caagaactct agggtacatt tctactaatt gctggatagc agggtctgga
    24661 atcgtctccc atggcttgaa ggatcggaaa aaaacctctg tttcatttgc aaaaacatcg
    24721 gttgcaaaac cagctaaatg attgcttttc aaagctctta aaatagcttc attatcttga
    24781 agctctccac gggctgtatt aatcaagatg ctaccttttt tcatttgagc taaaaatcct
    24841 tcattgatca tttgatcgtt ttcacctggg atatgaggta catgaatact gacaatatcg
    24901 cttttggcta ataactcttc aagcgagtgg aacgtcaata cttttttagc agcttctgtc
    24961 tgatgaatat cgtagccgat cactttggct cctagtccct taaacaaaac agcttccgtc
    25021 aaccctatcc tgcctgttcc tatgatccct actgtgcaat ttctgatttc acgactaaac
    25081 atttgctcat ccactcgaaa atctttgaaa gatgttcgga tcgtcgtata cgcagtattt
    25141 ctcaaaagca tcatggcaaa agtcaaagat aactctgcta tcgcatttgg cgaatacgat
    25201 ggtacacggg ctactgtcat accaaaatca gctgccgctt gtagatcgat atgattgact
    25261 cccacagtcc gtgtaaaaac ataacgaatc ccatattcct gcatttttgc aagattggtc
    25321 tcatctgcta tacaatttcc cctcaacaat acagcatcat ggtcctttgc tgacacgata
    25381 ttgtcatgtg tcaacagttc ttcataaaga ctgagatcat aatggtaaat attcaatttt
    25441 tcaaaaaaat ctatctcgtt cggacgaaca ccgtaacatg cgattctcat actttcttcc
    25501 tccatttctt atgaaaataa acctatgcca tgaatcattt gtaatacaac gatccataca
    25561 ggtgtcagta ccacagcaaa aatagttgac agtaaggatg cattggaggc taaaagtgcc
    25621 tccctatcaa aactgatagc ataggctgct gccactgttg cagctggtgt agccatcatg
    25681 ataacgattg ttgccagtgc ttcaaaattg actggtagaa tattcgttgc cgtcaaaact
    25741 gctagaatca cgatattcaa tgcaggtaca aataccactt tgactagact gtaataccat
    25801 gaagtttttt cagatgcagc ttttttgaag cttacttcac ctaatgttgc gccgattgct
    25861 agccacgcca atggtgaact taaaccagct aaataatcca tcgactgaaa tagccacggt
    25921 gcagtctggt caattcgcaa aaacgctact tggcttatct ctccttctgc acctgtaact
    25981 gacacttgtg gcagataccc ttggaacagc caaatgaaaa ggccggcaaa agtagcgata
    26041 atgattggat tgacgaacat cgctttgata tttttagctt ccatcttcaa gccactcatt
    26101 tttatgtatc cataagaata caaaaagatt cggtagccaa tattgaaaat tgaggaatac
    26161 atcacgccga caggaccata aatcgcactg acgataggcg ttccgaaaaa ggtcgtagaa
    26221 ccaaagatcg tcaggacacg caacgttgtt tgcttatccc ctttatagct taagaaaaaa
    26281 ggttttgaaa ctagaataag aataatataa ataactaatc cccaaatcaa gacattgatt
    26341 ccttgcatca gtaattctgt attgatatct tgcatgaaag ctcgaaatgc cagtgctggc
    26401 aaagccactg ttaagacgac tttcgataga atttttccaa cagattcaga aaaaatcccc
    26461 ttttttctac aaacaaaccc taataatata ataaaaactg ttgaagtgat tgcactgaca
    26521 atcgacatat ctgtcaaagt cgatcgaaaa atgtcaaata agttcatctt tctcctccta
    26581 attatgttca tttattcaca
//
LOCUS       DASJUF010000028        26403 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJUF010000028 DASJUF010000000
VERSION     DASJUF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.028"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(194..1138)
                     /locus_tag="U4B62_001086"
     CDS             complement(194..1138)
                     /locus_tag="U4B62_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141259.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1359..2573
                     /locus_tag="U4B62_001087"
     CDS             1359..2573
                     /locus_tag="U4B62_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2141260.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2595..4610
                     /locus_tag="U4B62_001088"
     CDS             2595..4610
                     /locus_tag="U4B62_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN2141261.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4693..6066)
                     /locus_tag="U4B62_001089"
     CDS             complement(4693..6066)
                     /locus_tag="U4B62_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2141262.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(6092..6376)
                     /locus_tag="U4B62_001090"
     CDS             complement(6092..6376)
                     /locus_tag="U4B62_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2141263.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6378..8393)
                     /locus_tag="U4B62_001091"
     CDS             complement(6378..8393)
                     /locus_tag="U4B62_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2141264.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8572..8877)
                     /locus_tag="U4B62_001092"
     CDS             complement(8572..8877)
                     /locus_tag="U4B62_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN2141265.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8966..9997)
                     /locus_tag="U4B62_001093"
     CDS             complement(8966..9997)
                     /locus_tag="U4B62_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2141266.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10204..11079
                     /locus_tag="U4B62_001094"
     CDS             10204..11079
                     /locus_tag="U4B62_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2141267.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11231..12073)
                     /locus_tag="U4B62_001095"
     CDS             complement(11231..12073)
                     /locus_tag="U4B62_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2141268.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12165..14042)
                     /locus_tag="U4B62_001096"
     CDS             complement(12165..14042)
                     /locus_tag="U4B62_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2141269.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(14039..15460)
                     /locus_tag="U4B62_001097"
     CDS             complement(14039..15460)
                     /locus_tag="U4B62_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2141270.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15463..18852)
                     /locus_tag="U4B62_001098"
     CDS             complement(15463..18852)
                     /locus_tag="U4B62_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2141271.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            19109..20500
                     /locus_tag="U4B62_001099"
     CDS             19109..20500
                     /locus_tag="U4B62_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141272.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20573..21220)
                     /locus_tag="U4B62_001100"
     CDS             complement(20573..21220)
                     /locus_tag="U4B62_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2141273.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21228..21914)
                     /locus_tag="U4B62_001101"
     CDS             complement(21228..21914)
                     /locus_tag="U4B62_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141274.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21920..22993)
                     /locus_tag="U4B62_001102"
     CDS             complement(21920..22993)
                     /locus_tag="U4B62_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141275.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23215..23889
                     /locus_tag="U4B62_001103"
     CDS             23215..23889
                     /locus_tag="U4B62_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2141276.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23894..25234
                     /locus_tag="U4B62_001104"
     CDS             23894..25234
                     /locus_tag="U4B62_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2141277.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25383..26369)
                     /locus_tag="U4B62_001105"
     CDS             complement(25383..26369)
                     /locus_tag="U4B62_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN2141278.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7690 a         4982 c         4838 g         8893 t
ORIGIN      
        1 tttttgatgg aaacgaattc ctgaatacgc ataacagata aatgacaaat gtttacacct
       61 atatcccctg ataatacaac caaaagttta gaactgaaaa taaatccaaa aaaattcgct
      121 gcaacgatat aataagacag caatcgcacc taaaacaatt ttccttcata tgctttagtg
      181 aaagattttc ttcttaatgt ttatttctcc attcattggg agtacatgtt gaatacttct
      241 tgaataattt gaataaagaa gtcgtttgtt tatatccgat acgattagcg atttcttcca
      301 acgaaaaact cgtttcttga atcagttcca acgcaaagtc gaattttttc tttagaacca
      361 gttcttgaaa ggaagcatca aaatacattt tcaatttatt actaagatag accggattgt
      421 agccaaaatg agctgctgtt tcttttaacg tacaagaagt aaaatgctta tcgatataat
      481 ctaatactct aagcatctca ttttctgcgc atccatctgc tgatgaaaag ctttgttgtt
      541 ctaataaatg agtcaattcg atcagtaata gtgatgtata gagatttaac gaacggatct
      601 tatgactgta tgtatcaaaa taaatcaaca gcatattttc aatcaaatgc gtcaaacgac
      661 ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc ccctttttct tttgatgcat
      721 ttatcaaaaa agaagaaagt agattatttg tgcttgccaa atgattcaat atctctgtag
      781 ataaggtttc ttcttttaat aagatgttga ttaaaatatc ctcttgccct gtgtattcga
      841 ttctttgagt agcttcttta tcaatgatca ccacgtcact ttcttccatc tctatccttt
      901 ctccattaat aatttgtaaa catgtaccgc tatacatata attgatttca acaaaatcat
      961 gtgtatgctc gggaacttcc gcatatcgac ggtgtttact gatatgaata tttccctgtt
     1021 gaaatagatc ccctcggtgg atttttggcg gtgtctgaag atggtaagct aagtttaaat
     1081 cattgatatt cattccttgt tcacgctgca gagtttcgat tgattcttcg ttacgcaaaa
     1141 aaaatgtcta attcagctag ttccaattga ctccctcctt tttgtaagcg gtttctttcg
     1201 cttattagtt cacgctgtac tgctattata tgcaaagcta tttttggttt cataaagact
     1261 ttctatggtt gggacatgaa cttcctgtga ttccgctatt gagagaataa ccacttcata
     1321 ctatattttt gataagcgct tacaaaaatg aaaggaacat gcttatgaat aaaaagatga
     1381 tgaaattttc tctgctgtca gtctctctta ttttgacttc tgctggtgca gtcacgtctg
     1441 caattcccag tatgtcagct acttatgcca cgcgttcttt atctgctgtt gaattattaa
     1501 cgactgctcc ctcttttatg gtcactattt ttgttttgat tagtagtttt attgctagga
     1561 aaatcggaca aaaacaaact gtgatcatcg gattagtgat tgcatcgatt tgtggtatcc
     1621 tccccctctt ctcacaaagt tacagtattg tcttagcttc aagagctgga ctaggtattg
     1681 gctttggatt gatcaattct ttggcagtca gcatgattgc tgccttttat cacggagatg
     1741 aacgagcctc tatgattggt tttcaaagtg catttcaagg atttggtgca gctttaatga
     1801 catttgttgc aggacagcta gtacagttcg gttggcaata taccttttta gtctacgcca
     1861 tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc tacacctgaa acaactgaac
     1921 ttcaatcgca tggcacagcc ccaacaagac aagtaatcaa aacgaatcct aaaatgatta
     1981 gttatggtat ttttctcttg ctggtcatcg tggtatataa tgctgtatcg attaaattag
     2041 ccacagtcct gacgacttat tctctaggaa acgaaactaa tgcagcgaat atcttaacaa
     2101 tcatgcaatt ggcaagtatg gctgccggag tttgttttgg ctggcttcag acaaaagcac
     2161 atcgctatat gcttacactt tctttactac tgatgggaat cggctttgta ttgattgcca
     2221 ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct aacaggaatt tctttttcgt
     2281 tattcattcc ttacttattc aaccaagtaa gtatccaagc gcccaagtac gcggagagtt
     2341 tcaatacttc aatcttattg gttggcgcta atttaggtac tttcattgca ccatatggtt
     2401 tgctctttct ctcttttgct tctcttggga gtaaaacact tgctgttttc gtcaatggca
     2461 gtatcttgct gttgattagc gcgcttatta gccttctagt agtacacagg acgaatagaa
     2521 agacagtgat aagagtagat gaagaaataa aagaacaatt gacaacaaaa taaatttgat
     2581 ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag atggctttgg tatcctggag
     2641 actttgagat ccgccatgga ctgctacaaa atttccaacg ggaagaacga ggttttgact
     2701 ggccagcgta ctggtacatg gatgattgcc atcggaacgt caagtttaag agatattact
     2761 ttcttgatca accttccatg tttaaagtga cgatccaagg agttgggtat gtagaaatta
     2821 atggacaaaa acatccatgt ggaaaatggc tgacttgtcc agctggtaaa gcaaaaattc
     2881 gtatctttgt aggacataca tcagggctac cagcgatgtt tatcgaggga gacgaagtaa
     2941 aatcagatat tggctggaca gcaagtaatt ttattgaaga atatccagct ggctggagtc
     3001 cgctttatgt cgatatcgca aaagatccaa atcaaatcta ttatcaaaaa gaatgcgtcc
     3061 atccagtcac tgaaacggag attggcggag gaatcttatt cgattttggg cgagcagtga
     3121 atggctgtgt agaactaata tctctggatg accatatcga atctatcact ctatgttatg
     3181 gagagtccga tgtagaagct atggatatcg attattgcta ctataagcaa ataggaatga
     3241 caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt gtttatacca gaggtttcta
     3301 ctggaatggt tcaacttcaa gtttttcatg aatttttatc tctttcgaaa caagctgctt
     3361 tttcaagcga ttcttcttta ctgaatcaga tctggtcagt atcagaagaa gccttcaagt
     3421 tatgtagtga tttgtttttt atcgacggta tcaaacgaga tcgctggatt tggtctggag
     3481 atgcgtatca atcattcatg atcaatcaat atcttttttt tgacgaggaa atcaataaac
     3541 gaacgatatt agcattaaga ggacaaaatg aaatcaagca gcatatgaat actatcgttg
     3601 actattcgat cttgtggttg attggtatcg aaaaccacta tatgatgacg aaagactatg
     3661 aatttttacg catcgtctac cctaagatgc aatcattaat gagatacttg atggagcaaa
     3721 cgaatgaatt agggtttatt tatgggagag caaaagattg gatatttatt gattggtctg
     3781 aaatggataa agaaggaacg cttgctgcag aacaagtctt attactgaaa gcctatcatt
     3841 ccatgatcac ttgcggagat atcttagctg aaacatatgg agagaacgaa aattatcata
     3901 ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa cctatttgac tacttttggg
     3961 atgaagaact tggcgctttt atcgattgct atgaatctgg gagaagaaat gtgacgcgtc
     4021 atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa agaccaacag caatcgatct
     4081 taaagaatgt cttattgaat gacactatca cacagattac tactccatat ttcaaattct
     4141 atgaacaaga tgctttatgc aaactcggac agacgaatat cgtctaccaa acgattcttg
     4201 attattgggg agggatgtta tctcatgatg cggtaacttt ctgggaagaa tacgacccta
     4261 ctcaaacagg aaatgaaaag tacagtatgt atggagatcc ttatggaaaa agcttatgcc
     4321 atgcatgggg agcaagccca atctatcttt taggacgcta ttttatcggt ttgtatccaa
     4381 caaagccagg ttatgaagaa tttatgatac aacctaatct agattcattt gaaacactga
     4441 attgtacgct acctatcaaa gatggaaaga tttcgcttga aaaaacaaaa gataagatcg
     4501 tgattacttc tgatagaaca ggcggaacag tgaaaatgaa tggtaaaagt tatcctcttc
     4561 tagcaaaaca gccacttgtc ttaacaagct attcaaaaat aaatgcataa aacaaaaaac
     4621 tgcttgcgac atatcagaag actttaaatc ttcttctgat attgtccaag caatttttat
     4681 tttgaagttt gcttaattct gttctttaga aacaatgttt gttctttttc cggcaaaaag
     4741 aaagctagat aatacgacaa gtaaaaagaa agcgatgatt ccaactgtca aggctatttg
     4801 accgtattct cctagtttcc caagtacaag tcctggtact agataatcag tatcggcaaa
     4861 tgtagaagaa gcgattttaa gatctcctaa tactggaagg ataaataatg gcagccaact
     4921 aatcaataag ctattgaaag cagaacctaa taccgcccct cttctaccgc cagtcgagtt
     4981 gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct actaccccag gaatgataac
     5041 tgtagtttgc aagcctatca tagcaaacat ggatagtgtt cctacaaaga agctaacaaa
     5101 aaagccaatt aatacagcat taggtgcata tggaaaaacg ataggaacat ctaatgctgg
     5161 tttagaatcg ggtacgagtt ttttagcaat cccttggaat gctggaacga tttctgccaa
     5221 caccattcga accccttgaa ggacgataac aaacccagct gtgaatgttc cagcttggat
     5281 cagtgcaaag attacagcgt tgtctccatt gcttagttct ctttcaacaa atccaggacc
     5341 agctaacaaa gcaagtgcga catatacgat cgacatcaag agcatgatcg atacagtaga
     5401 atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc tctgtagaac ggtctttgtt
     5461 tccaaaaaac tttccgatcc atccactaga agcataaccg atatttccgg tatgccctaa
     5521 cgcaacttga ttgtttccag tgatgttctt cataaacggt tgacaaagag caggcgtaat
     5581 agttaatagt gttccttcga agaccccgcc taataagatc atccacaaat ttgcagtaaa
     5641 ccccgcgctt actaaaatga ctgctgttag acaagaaaca taaagcattg cttgacctgt
     5701 aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa atattcacga tcatccctac
     5761 tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa gcaatcgcca ctactgcttc
     5821 attgctagga actactcctt gagtatgcag agctgtttgg aacatcgaag caaaaggggt
     5881 tagtgaatta ctgattaaac cagcaccgcc gatcagtact aggaatccga caaatgtttt
     5941 gatagttcct tggatcactt ctgaaaattt cttcttctga agaacgagac caagcagtgc
     6001 aatcaaagaa acgaggatgg agggctgact gacgattgac acaaacaaat tcaacaattt
     6061 atccattgat agttcctccc ttgaatcatt tttattcttt tgacaagatc ttcgttactt
     6121 tttcttttac ttcttcgctg tcaatgatcg attgcaaagg tacgagcttt tcatcaggta
     6181 tagaagttag cgtatctttc aaagaatgct caatgaagaa ataatcagcc atatcgctag
     6241 atacacttcc aacatccgta tgacttactt caaactcgtc cacatctgct ccaagatctg
     6301 caagtgcttg tttgatgttc atctctacca taaaacttgt tcctaatcca ctttgacata
     6361 ctgcaaaaat tttcatatca ttcgtctcct tttcttatga tcgctaatat ttcttctttt
     6421 gttgatgcgt gtaataaatc ttgaagattt tgtttattag aaagtagctt tgttaaattt
     6481 gccaaagccc gaagatgtgc ctcattatcg atagcagcta aacagatgaa caaactaatg
     6541 ggatgggctg gattatcaag cagatccaca ggttgttgaa gtttcagcaa agacatccct
     6601 acttcgttta cgccatcttc tggacgagca tgaggtaatg cgatgtgatc gccaatatgg
     6661 ataaacgcac catactgctc cacgcgctcg atcatagctt tgatataact ttcttctatt
     6721 ttattttctg ataatagcgg ctgtgcggca aatgtgattg cttcttgcca gttttttggt
     6781 tgatccgata attggatcat ttcttctgtt aataattcag atagcatagg acgttgatcc
     6841 tccttctttt ctaaaagttt gttcatttta cgatttaata cttgatagag cttttcttcg
     6901 tctacgcctt cttttaattc gatataaggc agtagcgccg acaacaattc ttgggcagaa
     6961 ggcattgaaa atcctggtat cagccaatct ctttgtactt gattgatcag ctgatttttt
     7021 tccaagtcag tcatgattgg atgcagaaca tagagagact ttttcttatc agtaagtgtg
     7081 agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt caacctcgtg taagcggtca
     7141 gctgaattag tctctgtaaa aaagatcatc gggaataatt gctgaagttc actttttaag
     7201 atcaatgaag agctaatgcc acttggacag acgatcacgg ctcttatttt tctatttctt
     7261 tcttctgcgt ccgcttttct gatctctccg ccaaatagta tagtgaaaaa accaatctct
     7321 tcttcaggga tgctcttccc tattcgtctt tctaacggaa gtaatgcttt tcttgtcatt
     7381 tcgtagatgg aagcatattg atgcttaatt tgatcgatca tcacattagg taaataaaaa
     7441 gaataggaaa tacgaaaata ggcagggacc aagtgataaa agacattcat caataattca
     7501 cgaaacgaag caaattctac ggccatcagg aattccatgc gatggatgac ttctgaagaa
     7561 cattccagca aaaaatcaag tgcaggctcc tggacatctc cttcaacgat cgatgctaag
     7621 caaagagaga aaaatataat ctccgaattt tgatgttcgg aatagccaag gccagtcaat
     7681 acttgtcgac taatgtcagt gactgcaaga gaagccaaca gttcagcatc ttttaccagt
     7741 tccatggatg tttttccaat tcgaatggtt gtcaaactca aataataagc taattcatcc
     7801 attcgatttg gtgcgaatcg aagatctgat agtgaaatcg ttttatctag caaatctcta
     7861 actttggcgt aataatcaat ggaaagggtt gcggttaaat acgtgagccc aaatcttcca
     7921 gcaagtgact gcatcaaacg agcagtgcga ttttttgctt ctcttcttaa tattgcttct
     7981 gaccctagca gcctaaaacc agttttccta gtatacgaca gctgacaatc ttccacttct
     8041 aattcatttc ttatcttttt gatatctgac aggactgtcc ctttagaaac ctgaagaaaa
     8101 tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga
     8161 tctcttcgct cttgttcggt atacacagct tcaaatcgag taatatccaa aaatttttct
     8221 gcccagtcga ttgctcggaa tttctttata cggatacggg tatcatccac atattcggca
     8281 aaaggagtta attgtttgat cgcctcgctt gtatttactt cgatgatctc tgctgctttt
     8341 tctagactga tttctcttgg tgttttcagg agataactaa ttttgtagtt cacagcgctg
     8401 ttcacctcct acttatattc tagtatggta gcgcttttaa atcgaggggt gtttggtaca
     8461 acttacagtt ggattttttt tacgactgta ccgaacgatt tctatcgaat ggatacagaa
     8521 aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg atttcgtccg atcaatgatg
     8581 atcatgttta ttttttgagg tcagttcttc aaatttttct ttgacatctt caaccgcttt
     8641 ttcgatatct ttttcaaagt gattgggctc attaggcttt gtttcctctt ctacagaatt
     8701 tgaaccagat aaagctgttt ctttattttc tgtttcacca gagtttgtgc cagctaatga
     8761 ttcttctttg tcttctgttt caccagaatt agttcctgct aaagccactt cttcttctgt
     8821 tttaggggcc attgaacctc ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt
     8881 ggttcctcct agtagttcta tactttttca tcgtacaata agacagttta tgcgtcaaaa
     8941 aacaaacccg attcttacaa atatgtcata tttttccttc tgattcttga atgcttttga
     9001 ttccttcttc caaaattttc atttgtaaga ttgtttcttc atctttgatg actttttctg
     9061 cgccattaac taatgtctcg taaatccctt cgtagacacg gctgtaatca ccaacctcgg
     9121 aaattacttt ttcttcatga taactccctt gttcatctat ataggtcaat acaccataat
     9181 gttcgggcaa atcgatacca aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt
     9241 cttcttgtcg atcttttgtt tgtttcacga acatcccttt ttttccgtaa acgacgaaac
     9301 ttggacgttc tttgatgcgg aaataactgg attttatcga aattttcata tttccataga
     9361 aaaaatccag atcgaagtaa tcattcattc gtcctttccc taaaagttga cgtacatcat
     9421 agctgatatc atctggttcg ccgaaataag aaagaacttg atctactgta tgacaagcat
     9481 gtccatataa atagctgttc gcagtagaaa attcatgcgt attttctggt acttccggtc
     9541 tgaaataatc ataatgcatt tctacttcta aaatatcccc taattttccg ctttcgatta
     9601 ctttttggac tgtcaagaag tcagaatcga accgtctgtt ttggtaacat tggataaata
     9661 gacctttttc ttttgctaat gcaaataact cttctgcttc ctttgacgtt tctgcaaaag
     9721 gtttctctac tagtacattc ttaccatgtt cgagtgccat ttttccatat tcataatgaa
     9781 attgactagg tgtagtgatg acaactaatt ggatctcagg atcatcccaa agatcagaca
     9841 gctgatcagt atattcgatg tcttcgaatg ttttccacac tggttcagat ggggaaaaaa
     9901 tttttttgat attgattttg ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg
     9961 tactctttcc attgccaaga taagcgattg taagcatgag tggtgcctcc ttttattgat
    10021 gttttcagta tatcctagaa aagcgcagaa agtatttata atagctattc tggtaaaaaa
    10081 gttcagaaaa acacttgtta tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa
    10141 aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta agatagaatt tggaggtcag
    10201 atcatgctta ttgaagaaaa actaacaaaa caagagttgt ttaccacgac agagaaacgg
    10261 attgtcgatt acatccgtag gaatatcgaa gctgcagtct acatgacgat tgaagaatta
    10321 gcaaaagcta cgtatacttc ccattcagct atcattcgtt tatgcaaaaa aatgggattc
    10381 agcggattta aagaattccg tttcgagctg gggcgggaag ttcatcaatt gattgctcag
    10441 ttcaatcaaa cgattgatcc taattttcct ttttcaagtg aagatagcac tcagcaaatt
    10501 gcaaagaaaa tggcggattt atcgatccag tcaataaaaa aagcacaatt gcagattgaa
    10561 catgaagatt tagatcaaat cggtaaagta ttgaacaagg cacagcgaat ctttcttttt
    10621 gcaaaaggtg attctcaaat cacggcaagg aaattccaga ataaaatggt caagttgaat
    10681 aagtttttga tcatggctga agaatacagt gattcttctt ggaatgcagc caatttgact
    10741 agcgaggatt gcgccatttt tattagctac agcggtcgca tccatcatta tgaacgaata
    10801 atgacctatt tgaagcatgt agaggcccct accttgctga ttaccggaaa tcagcattca
    10861 gaaatggcca aacaggctga tatgtgtctt gttatatcgc aggaggaata tgattttgct
    10921 aaagtagcga ctttctcctc tcaaattgcc ttcgattacg tattaaatac gttattctca
    10981 gtgatatatt ctcaaaattt tgaagagaat atccgcaact tgaaaaacaa acaagaattg
    11041 atgcaaaaag gccttcttaa agaatatcgg aaaacttaag aaaagatcct catttagtaa
    11101 taagaaagat agtgtttgat aggctctctt tgatatcatc ataaaaacag gctgaagttt
    11161 tctttttcaa acctcagcct gtttgtttac ggaacgattc gaatagatat attatagaaa
    11221 tgaggaatag ctaacttctg tttcttattt tgaacgattg acgaaaaaga tatataagaa
    11281 ggaggaaaat cagtatacat gcagctaaca atccatacag gaaaaatcgt ttgttctcgg
    11341 atactttctg tacttctttt tgaacttctt ttgcatgata aggaacccgg tgccctctga
    11401 caagtaagcg atgactgttg accatataag gtgtacaagt taggagtgtc acgaaatcct
    11461 gccctttttc gattctcaga tcttttgtat cagtcggttc taccgtcttg atctgatcga
    11521 cttgataagc gagtgtccgt ccattgattt cgatatagaa agaatcacct aaagtcagtt
    11581 tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg atggctggaa ataacggcat
    11641 gtgtattttc tccccccgta ggataagacg ttccttccaa taatgaggca cctttttcta
    11701 gcagcacagt attcgtttca tcaaaaattg gcaaatggac attgatctca gggatcgtca
    11761 aaataccgat cgtatgtttc tcaaaatagg ttctatcctt tttcgcaggt tcttttttct
    11821 tagtaaaggg atctgcacct ggatttccac cttcttttgc taattgctgg tttttctttg
    11881 tcatgttttc ttggatttta gccatgactt cttcattttc tttgaccgct tgttgctgat
    11941 aatgagagat gatctgctga tccagataat tattcagcgc gtcgctaaca aatggataag
    12001 ctagtatccc tattcctgag agaagcaaga aaagcatcag cagatctaga agtttccttt
    12061 tctttggttt catttgtgtt ctccttgtga tgactgtttg ataataaaag aaaaaggagc
    12121 atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc
    12181 ttgcgtctag caaagtacag tccagcaatc agtagaagga ctactcctgc accgatatac
    12241 acatagattc ccttaccgcc tgttgaagga agtgtacctt tgtgtttatt tggtattttt
    12301 tcaggagaaa tcaactgtcc tgctgttaca taagattgtt catcgatagt aaatgctaca
    12361 cggtttgtta atggaacata tttttctggc gctttcgttt cttccagata gtacgtgcca
    12421 tattttagac ctgtcacatc gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat
    12481 tctttcgccg atacccagct gacggctttt gtggatgggt cgatcgataa atatttcgct
    12541 gtgtcactat cttgatcacg aacgacgaat tctgctccag caagtgtttg gtctgatgta
    12601 acgtcaccat ctactttaac aaatcgtttg cccccagtaa cgacatcgac tgacggtggt
    12661 gtttgatcat ttgtatgccc gttatcgaca tttgcttggt tagaaaatcc tttggttgga
    12721 tctgcttttt cgttcaaatg catatagtaa acgaatttca atgtaccgcc aggagttaat
    12781 gaaggaatat aattcggatc aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa
    12841 taatttactg ggtcgatttc tttatttcca tcatataagg cataagcata cgttcctgaa
    12901 gaatcattat caaatgttaa agcagcgtca tgagtatcga taagtttgaa tgttgtgtac
    12961 ttgttttgcg ttccttcttt atcagcgata cctaatggga tattgacaga aatctcatat
    13021 tgaatttttt caccgattgc gacatctttt ccattcaatt gaggtgttgt tttatcaact
    13081 ttagacgtat catttttgat ggtcttttct actggtgttt ggctatttgc caggatctca
    13141 aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat caggagcttt tacttcttct
    13201 aaattatatt ttccaacttc taaatgatta acgatcaact ggcctttttc aatagatgcc
    13261 tcggcaaagt cattgttgcc gatgtcataa gaatgaccag taatgaaatg tttggctttg
    13321 gtttgatcag ttgtccaagt gtacaatcca tctgtgacac tttggatgta ttttttgacg
    13381 cttggtgttc cttcttcttt agaaataata aattctgctc catttagtgc ttcgttttcg
    13441 gcagtaccga tttttgtaac tttcaacgtt ccatcattac cgactgtatt tttagggtag
    13501 agatggatag tatctagttc ttctgtcccg tatttataag agccatctgc ttgtttgatc
    13561 atctcatata cagggaaagc taaaaccatg tttgcggcag ctgacactcc gtcttttggt
    13621 tcttctttga tcgtgtagac tgcatctttc ccattttgtt ttttaggtaa agataaagtg
    13681 acatttccat cagcatctgt cgttcctgaa gcaactggtg taccaggagt caaagactgg
    13741 actgcttgtt ttgctgcatc cacggacgct cctttatctc gttgcgcata aaattcttga
    13801 gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt ggtattgatc gaattcgctc
    13861 atttctttcc cgctgttttg gattaaagga tcgggtaaat cagtcatttt ctttttatga
    13921 aggatgactt gagctgctgt ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg
    13981 agtaatggta aaataaggaa aaggactcct aacataacgt ttattttttt atgatttttc
    14041 attttttcac tcctgttctt tttttgataa acaaccagat tgcaaagctt gcgaatacca
    14101 taccaacaac gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga aatggacggc
    14161 tattttcttt tttattgact actttcaata tactgtccgc ctctttatcc tcagaaagaa
    14221 aagttgaaat ggtaaacgga atttctgttt cacttaaaat ataaccagaa ggtgcttcga
    14281 tctcttttaa tcgataactt cctgttttca acccggaaat tgaaaaagcg ccatttttat
    14341 cggaaatcag gtgaagcgca tctgattcgt tctttgtcca cttgtatccg ttggctgttt
    14401 gctggaggta atttcctgct ttgtttttaa taaggaatac tgcgccagct aatcgtttat
    14461 ctggtttatt tgtctcgatt ttgacaaatt gcttactccc tgtatataca gagactttct
    14521 ttgtactgac accaaaacca aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct
    14581 gtgctgtact gcttagctgc atctgataag aaatggtcag tttttttcca gcatattttt
    14641 ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc
    14701 cttctgtcag cttttctcca ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg
    14761 cttcatccgc actatctgac aattcgaact tttgatagtc aaggatattt acaggaattg
    14821 tcgtatgcaa ggaataagtg attttttcac caatcgtaaa atcatttcta ggctcttcta
    14881 ataccttctc aaaaggtgga tcagggtagt cgttttcttc atttttagga tacaaatgga
    14941 tagttgaaag tttttgattg ttttgtccat gtacaggaag aacaactacc atattctctg
    15001 ccttttcttt gacgacttct ggtactttgg attcaatgaa aagatacgct ttatctcttt
    15061 tttcttgatc tttagcggcc agagaaaaag aagcgatacc atcttcgttg ttaagagttg
    15121 tagttgttgc ttctgcggta aacataccgg aagacgcacc gttttttgcg atctctgctt
    15181 gtgcttcttc taccgttttg cccttttccc gtagatggta aaaagaatct gtgacatcat
    15241 aaacttggaa tgtgacacca tttaatcctc gatacgtttg taataaagct ttttcttgtc
    15301 cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa
    15361 tctgaaccat ttctgtttct gtttctgttg ctacctgagt aaaagctggt ttaaatagta
    15421 acaagaagag acacatacta agccaaccaa gttttttcat atttagctgc cccctctttc
    15481 ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca atcatcccta ctagatagat
    15541 tcctaaacgg ccaattcctc ccgtttcagg taatggtact tttgcctgat tcgtgatgtc
    15601 taaagaaatc tggttgtttt tggctcctgg tgacagaaca gattcatgat cttgtccatc
    15661 cacttgaatt gacccatctt cgtgtataac tatagtaact ggctctttta gaccttggta
    15721 tccttctggt gtaaaagttt cggtcagcgt ataagttcca ggttttaaat tttcgaatag
    15781 aaaggtatct gtttcttgtc catcttttgg cgattctaag tccatttctg gtccctgcag
    15841 tctgaatttt gctcctttta atgtctgtcc gttgtcttct tttttgtgta ccgttagatc
    15901 aaacgctttt agtcgatttt gatgagtgag cgtccataca ttatttgtca tctcggtatt
    15961 ttttcccgtg taaatgattt ttccatattt atcgatttgg aattcataat ttcccggcga
    16021 agtatcatat ccggcaggac cagctgtttc aacgactcga tatttaccag gtgtgagtga
    16081 atcaaaactg gcaagacctt tttcattcgt tgtttggctg tcaattggct gataagtacc
    16141 atttgcttct tttttctgta gggaaaatgc tgctccttta agatttatct ctttgtccgt
    16201 cccttgcatc gcatacttac gtactgctac aggaacttca acaaaaggat tttcaatagt
    16261 caactggatc gtatcatcgg aagtagtgac tgtttttcca tcaatggtta ccgtaccatc
    16321 agaagcgatc ttgatttccc aagtagtctt tttgcttaat ccatgccctt ctggagcttt
    16381 tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg acattttctg gaagagaata
    16441 ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa tctcctgtta atttgaaaac
    16501 agcgccaata agatcctttt cacctgtaga ggaatttttc gttattttca agtttaacgg
    16561 tgtgaattgt ttagtatttt tccatgtcat tgcatctatt tgttgagaat cgtatcctgg
    16621 tacaggtaat tctttgattg tttggtaact gaatgcttga ccttgattat tgtattgagg
    16681 tagtgataat atctcttgat aactttctcc gctatttgca gataatttgt caatgtctgc
    16741 acgttcccac gtatttgttg tatctttagc tggtttaatg attcgaatat atccgttttt
    16801 ccaagctgca gcatttgtcg tatgttcccg ttgaatctca aaagtaactt gatctggacg
    16861 atcagctaga ttgttgtcaa attcttccca taactttttg atgtgaagac tgactccggg
    16921 agctttagca gatggtatac cgaattcagc taattcattg gtatcaatgt ttggctggaa
    16981 agttgtcctg ccgttcattt gataccagaa atttggatgg aagtcctcat tttctgtttg
    17041 gattctcact tggtaatgga tttggatttc ttggtctttt cctaaataga tctgattgct
    17101 cttgatggta tttccttcta tggaaataga tggagtgacc gttgtaggac ttgtccccac
    17161 actcttgact gaaagtgtac caggctgata aatgaatggt tctgcgattg gatcatttat
    17221 ttgtccattg cttacagttg ctgagatctg tacagctttt ttggctaggt attcagagat
    17281 atctgttgca tgatcagctg attcatagta aagatcgcct ttttcatctg atgaaaccat
    17341 ttgtcgcata cgagactcta cttctgcttt tgagagacca gctgccggat cgctttgaag
    17401 ttggatgcca agaccatgta tttcgattcc tcgttctttg agtgccatcg cttctccgat
    17461 ggttgcgata aacgtactgt cgattcttct ggatagattg ttttggtcag gtgcatcata
    17521 gattcttgag attagagaag tatttcccgg ccgatcttgc gtattagaaa actgagttcc
    17581 gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact ttataggaaa atgttggtac
    17641 accatccgtc agcaaaacga tcactttttt atgtccattt ggaacggata gcatgcttcc
    17701 tgcatctctt agtgcttttt gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga
    17761 ttttacttga tttttcactg aatcaaatga acccatctgt actgcaccgt tactgtagct
    17821 atatccttcg cttgagtagc cgacatatcc catattgatt ttgtctgtga taccgctatc
    17881 tgctaaagta tcgacaaaac gatcgacacc aatctttact tcaccgatcc gattattgtc
    17941 gttcatactt cctgaccagt ctacgaccaa tacgagatca agaggcgtga tatctttttg
    18001 tacatttcca cgagcattca aataaacatc aaacaatccg ggtgtacttg tttcctttgc
    18061 atactttcgt atcgcatagt ctgcttgatt accggttcct ccatattcga tatacgaatg
    18121 ggtccgatca ttaggatctc cattccaaga atttatacca tcccattgat ttgttccgtt
    18181 tttattgcct tgatgatcaa gaacatttgt atttcctgta ggttgccaac tggctttagg
    18241 ataagtgccc tgatcatctg ttgtatattc tgtaggaatc gtttgattag atggcacact
    18301 cacagctggc aaggataatg taggcaatgt aaatgggctg acagaatttt cgttttcttt
    18361 tgatgattct tcttctattt cgtctggctt tggtactgac acatctgtat ctttcttatt
    18421 ttctgtctca tcaataatgt catattcttt attatcgacg atcagcttgt ttgcctggct
    18481 gtggtaaagt gctggatcga ttggaaaact cagatcgata gtttgtttag tgtttgatga
    18541 gatttccaac gtaattttcc cgctatttac ctgataagca tagccatctc cttcttttct
    18601 ttccacatca cttaagtaat tttcgactag accaatttct ttttctatct tttcagcact
    18661 ggtgttattc gcatttagct tgatgttcag tgcttttttt ccagactgat ccgaaatgac
    18721 ttcagctgaa attgtttcta aaactgttgg atcatctgta gtgtctgcta ttgcagataa
    18781 tggcacaaag tttgataaac tcaatattat cagcatgaaa ataactttca gtttcttccc
    18841 tgtggtcgtc atttttcccc tcctttttct ttttttctgt tttgacgtga atagtgaaaa
    18901 ttattaaaat aaacaggcgt tacataattt tatttatttt aatatgataa ttattatacg
    18961 aagggcgcta aagggaatca caccaatttt cataccaatt ggtttcattt atgaagttga
    19021 ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt gagggagaaa aacatatgat
    19081 caggttgttg gaaggaaact atagaaagat ggaaaaaatc attcgaattc tgtccatttc
    19141 tgacaagtgg atgactaaaa aagaacttgc caaacgaatt ggtagttcag aatctacatt
    19201 tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc
    19261 acaaaaattg gggtatcgtc ttgaacattt gaatgtacca gcttatttaa atgttctgat
    19321 ggaaatgtcg caaagttcta caaacagtcg tttattaaat gagattattg tcaatccggg
    19381 taaaacgatt gagtattatt gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa
    19441 gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta aaagtgatcg ttgaccaagg
    19501 ttttcagtta actagcctta aaagtgagct ccagttgcgt atctttgtta cctttttctt
    19561 tttaagttac tacggaagac gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga
    19621 aattatggtc aagaataatt gtcagatgaa tttgtttcag gaagaaagcc catatgagca
    19681 atctttcttt attatgtatt ttgttgtgca tctcatgaga gaatcccaag gaactccatt
    19741 tgaatggaaa gctggagact cttatgtaca gttgataaat atcgataaaa aagacttctt
    19801 aatcttgaaa aatctcttag gaaaattgaa gtacagcagc tatataaaag tagtgaacta
    19861 ttttgtcaat acgatttttt cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca
    19921 gatacgaaaa aatatgtcct actgttcgtt ttcatccgct gattgcaaag aaaaaaacga
    19981 agaatatgtt gttcaattat taacgcaaat ttattttttc tcccgtattt tcccatacga
    20041 tacgactcat ttgacgatcc gtgcaactta ctttgcacag cagatgcaac accgccagat
    20101 acgtctatat gctggatttc gtgcagaact tcaattttat tccactctac tgaatcagaa
    20161 tctgatttat tatttatcta gctatatctt ttggatgctt atcggtaatc ctgaaattca
    20221 ccattatact tctgataaaa aaatactgat tatcagcgac ttaagtcttc gacatagtca
    20281 atatattgaa gaatatatca gtgatattct tgctgtgcat aaaattcact ctgagacaac
    20341 tgctattact gaagatcagc tgtcgaaata caatcttctc gagtatgatc tgatcgttac
    20401 gaatcaaccc attttgaatg cccatgttcc tcatatattg attgatgata gtgtgtcatt
    20461 cgcaaatgaa gaagaactca tccgcttatt cgagttatga ataatcaaaa aacagctatg
    20521 tataaaccac cctgataaaa atttcaggac aaatacacat agctgtttct ttttattgta
    20581 ttccttcagc aatccaacga gatttatagc gaaagtcttt taacaactct tcttttgtcc
    20641 aattatttgc aatgagatcc gtcaaagtgt tcattacgag ggtcatggaa aagtatagga
    20701 aactaactgc ttcttccgac gattgaatgg agaaatgatt gattcttaat aattccagcc
    20761 atttttcgtt cattggagaa tcagccgata gttctctacg tttggttgtc tgctgatcat
    20821 ttcgctgggt taacaagtat aacctttgcc aatagatact ttcgtgatct aagtcgcagc
    20881 accactctaa atatttttcg atccccaaaa agaaatgttg tttgttttcg ctaatgaatt
    20941 ttaagatatc ttgatggact atgtgagaac tgcttttgat cgtatacaaa taagcatctt
    21001 ccaaatcttg aaaatattta taaaaagcac cgcgcgacat ccccatcttg tcaacgattt
    21061 ctgagacttt gacttggctg atgtgctgtg agcaaaatat ctccagtaat aggtcatcga
    21121 ttctctgttt ctttgcgttc gataaattga aaaaagtttc ttttggcatc cacatctctc
    21181 ctttatctct gattatacgt ggatagccct tcattgtcat gaaggattca ttccttcttt
    21241 ttctcttctt ctgtcaagta cccatcttcc attcgataaa cgcgatcact ccattgaatc
    21301 atacggacat catgagtaac cataatagtc gctttttgtt tctcatgagc ttctttaact
    21361 agtaatttga cgacttcata tgcatgatct gtatccaaac tagctgttgg ttcgtctgct
    21421 aagatcaagc ttggctcatt atataaagcg cgagcaattg ccactctctg tctttctcca
    21481 ccagaaaggt ctttaggata actattttta agtgactgga tatctaaaga ttgcagcaag
    21541 gaatcgattt ttgttgaatc ttcttttttt gaagtaactt ggtcaactag ttcaaattgt
    21601 tcctttaccg ttaaaaaagg aatcaaatta gagctttgga gaataaatcc tatttcttta
    21661 aagcgtaaat ctgctcgttt tttttctgga agctcagtta gtgagtgacc gttgatcact
    21721 atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg taaggaaagt acttttacct
    21781 gaaccagaag gaccaatgat gctgacgaat tctccttcct ttacataaaa atcaattgct
    21841 ttcaaagctg agacctttgt atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt
    21901 aatatttttt tcatttttat taacctccta ttgctgtaat tgggtctact ttggttatcg
    21961 tacgtatcga aaacaatccg cctagcattg cgacaatgat caaaatccca ctatatagca
    22021 accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat
    22081 aagcaaacaa aagagcaagc agaacgccta ccaccccaac aataaaggac tgtgctacta
    22141 aagatttagc gatgaagaaa tttttgatcc cttgtgcttt cattactcca aaaatagcag
    22201 tcttctgaag tgtcatcacg tacatgaaaa taccgaccac ggcagcaacc actaagaaca
    22261 aaaagtatat catcgcattt aaggtcatat tttgggcaga atagccagga atactttcaa
    22321 tgaatgtccc aatagttaat aactgtagac ccttcgatgc tttttctttc gaaattttag
    22381 ctgaatcctt tattacgatc ccattgatcg gctgttcttc ttcagaagca aacggctgtt
    22441 gtccatactt caaagcagtc cacgtatcta gggacgcata gatgacggga gaaactgtgt
    22501 aataagtttc aggaaagata ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga
    22561 tcgtatctcc aattttatag cctgaatcag ccagattttg agagatgatg atttcattct
    22621 ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc
    22681 cgaaaactgt aacattttct ttgttttttc ctttgattgc tccgctgtac aatccgattg
    22741 gtgctttctc tcctccttca atgtatttca gatctcctct tgttaattgt gaagctgcaa
    22801 atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt
    22861 ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa catgaatacg acatacgcaa
    22921 tcaagaacat aataccgacg atcaagccat atcgtaattt agaatagcga atttctttga
    22981 tagctagaaa catttgtttt tcctccctat gatagattga tagtgacaaa atgtcacttg
    23041 ttgaatagtg taactctaaa gtgacaattt gtcacttgtg ttgttttttt cttatcaata
    23101 aattgtctaa gacatctttc gataaaacct taacatgagt cataaaaaat ttatttaagc
    23161 cttatttatg ctttccatga taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt
    23221 aaactattag tagtagaaga cgataaagta ttatccaata atataaaaga aatactcgct
    23281 gcaatcggcg agataacaca agtttatgat ggtagtgaag gactgttcga gttggaaagt
    23341 ggagtttatg atcttgcaat cttggatttg atgatgccgg aaatgaatgg atatgaagtg
    23401 ttagcaaaaa tacggaaaga aaaaatacag actccagtat tgattttgac tgcaaaagat
    23461 agtttggaag ataagattga aggatttgaa cgaggtgcag atgattatct aacaaaacct
    23521 ttctatcgag aagaactgat catgcgcgtg aaagcattat tgaaacgctc gctaggtctc
    23581 tttgatgaaa atgttctgaa gtataaggat atcagttgtg atttaaaaag caatgaagtc
    23641 ttgtataaag aacaagtttt gcccatacaa ggaaaagagt ttgaattgct cgtttatttt
    23701 ttacaaaata agggaatcat tttgacaaaa gagcagatat ttgaccggat ctggggattt
    23761 gattctgata cgacgattac agttattgaa gtttatatga gcaacttacg aaaacatctg
    23821 cgaagtactg gattggataa agaaatcaaa accttacgta atgtcggtta tatactgcaa
    23881 agggtgtgat gcaatgaatg gagatattac acgaaagcaa cgtagtcgct tttttgcgac
    23941 aaatgtagca gcatttgctt ttatcttttt tcttttggga ttcattactt tacagatatt
    24001 aaaccaatct gcatacagag aaacagatct gtcattagag aaaataaatt ttgattctcg
    24061 aacagtacaa ttagagatac aaagatatca gcaaggcgat ccctttttaa acccgactca
    24121 aacggaacaa aacagaacaa acatggaaca gccattaatg gaaccatcaa atgaccgttt
    24181 taacactcaa gtgatcttgt ggtctaaaaa tggtgagata ttgaataaag cagctattgg
    24241 tgggcgtttc aaccaaatca aagaattaaa aatggatacg aaaaatctga ataccatcca
    24301 aaatattgaa ttaaatgaag acagtggctc atctgccctc tctttccgtt cgattacgaa
    24361 aaaagcggag acgaatcaag atggcgttgc ctacatccaa attttagaaa atacaaatca
    24421 aatcaagaat tcattagcga cttttcgcac gattatgatt ttgtgtatga ttgttttttg
    24481 gattctctct attggtatca gttattactt gtctagtcta agcatgcgcc ctattattct
    24541 ttcttggaga agacaaaaag aatttgttga gaatgcttct catgaattgc gcacaccgtt
    24601 aacgattatt caaaatagtt tggaacatct ttttaccaag cctaatcata ctattattga
    24661 agaatccgaa agcatcgctc aagcattgtc agagacgagg cgcctcactg gattgacttc
    24721 ggatttgctg acaatcgctc ggaatgattc tgatcagcag ctgctttcaa agcaaatgat
    24781 caatacacaa gaatatatcg aacaacttgt taaaccattt caagagatgg ccatcataga
    24841 tggaaaagag tttattctcc ataacgatgc aacattagaa gtaccaattg atgaaaaaaa
    24901 aatccatcaa gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga
    24961 taagatcatt ttggattcat ctgttaacca taaagaatgg ctgattcacg taaagaatac
    25021 tggcccttct atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga
    25081 tcaatctcgg gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt
    25141 aatcgaacag catcatggac agatttctgt atccaattat cttccacaag gagtcgattt
    25201 taaagtccaa ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga
    25261 caaaaatctt gtcacagccc ttttttgttg cgaataaacg gtgttccaga agtagcttct
    25321 tcggaaataa gccgaaattt ctttaagctg accacttctg tcacaacctc tttagcttac
    25381 gcttacgatt ttttattgcg gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat
    25441 tcttctcggc taacatcttc tgtcactttt tcaacatctt catgttcttc tttatgaatc
    25501 ccaacttctt cacgaataac tgtatgtttg cttacatcta cttgctcttc tttgattggg
    25561 atagtaatcg tgtcatcttc caatttttcg tctgttccct gtgaagattg atctgttact
    25621 ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg
    25681 ttgaccacat gtttgtggat gtcgacttct ccagttgtca catcatgtgt atttacatct
    25741 aatttttctt cttttagttg aattgtatca tcatctagat ttggtttgcg atcgatgtct
    25801 tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta cgtccatcgg atcagttcct
    25861 ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt
    25921 ggatctgttc cagtgccagt ttctaatcca gtccccattg tgcttgctgg gctaacagta
    25981 tcttgtgtat ccattgtatc ttctgttgtt tctggctgac gatagccttt tactgtgatg
    26041 acaaattttc catcagaaat atctcctcta tattgatata aaggatcatt ttctggtgta
    26101 gccgtctcag aatcataggt atcaaaagaa aaagtatctt ttattttttc ccataaagaa
    26161 cggtcttctt caccatccat ccgattttca tctgtttcaa catctatacc agacaatccg
    26221 tcaaatccta gtctagatgc tgcttcatca gtagtataaa taacgatatc atctcgttca
    26281 tacccttcgt tacgcaaccg ttcaattact gataatgctt cttccctcgt actatagctt
    26341 ccgatgaccg ttaaattttt ctcttccatg taaattcctc ctagcaaata gatttatgat
    26401 tgg
//
LOCUS       DASJUF010000029        25959 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJUF010000029 DASJUF010000000
VERSION     DASJUF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.029"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(104..1336)
                     /locus_tag="U4B62_001106"
     CDS             complement(104..1336)
                     /locus_tag="U4B62_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN2141279.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="U4B62_001107"
     CDS             1591..2160
                     /locus_tag="U4B62_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2141280.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="U4B62_001108"
     CDS             complement(2338..2778)
                     /locus_tag="U4B62_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN2141281.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="U4B62_001109"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="U4B62_001109"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN2141282.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="U4B62_001110"
     CDS             3732..4655
                     /locus_tag="U4B62_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN2141283.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="U4B62_001111"
     CDS             4731..5870
                     /locus_tag="U4B62_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN2141284.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="U4B62_001112"
     CDS             5863..6663
                     /locus_tag="U4B62_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2141285.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="U4B62_001113"
     CDS             6663..7490
                     /locus_tag="U4B62_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2141286.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="U4B62_001114"
     CDS             7468..8202
                     /locus_tag="U4B62_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2141287.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="U4B62_001115"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="U4B62_001115"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN2141288.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="U4B62_001116"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="U4B62_001116"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN2141289.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="U4B62_001117"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="U4B62_001117"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN2141290.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="U4B62_001118"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="U4B62_001118"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN2141291.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="U4B62_001119"
     CDS             11787..13820
                     /locus_tag="U4B62_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141292.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="U4B62_001120"
     CDS             13864..15144
                     /locus_tag="U4B62_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN2141293.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="U4B62_001121"
     CDS             15354..16160
                     /locus_tag="U4B62_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141294.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="U4B62_001122"
     CDS             16172..17392
                     /locus_tag="U4B62_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141295.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="U4B62_001123"
     CDS             17382..18968
                     /locus_tag="U4B62_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2141296.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="U4B62_001124"
     CDS             19007..21145
                     /locus_tag="U4B62_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2141297.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="U4B62_001125"
     CDS             complement(21513..22496)
                     /locus_tag="U4B62_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN2141298.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="U4B62_001126"
     CDS             22874..24265
                     /locus_tag="U4B62_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2141299.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="U4B62_001127"
     CDS             24281..25321
                     /locus_tag="U4B62_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2141300.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..>25959
                     /locus_tag="U4B62_001128"
     CDS             25340..>25959
                     /locus_tag="U4B62_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2141301.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEK"
BASE COUNT         8261 a         4368 c         5295 g         8035 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaaca
//
LOCUS       DASJUF010000030        25756 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJUF010000030 DASJUF010000000
VERSION     DASJUF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.030"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..457)
                     /locus_tag="U4B62_001129"
     CDS             complement(<1..457)
                     /locus_tag="U4B62_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN2141302.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRR"
     gene            complement(461..1321)
                     /gene="rlmB"
                     /locus_tag="U4B62_001130"
     CDS             complement(461..1321)
                     /gene="rlmB"
                     /locus_tag="U4B62_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN2141303.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(1314..1709)
                     /locus_tag="U4B62_001131"
     CDS             complement(1314..1709)
                     /locus_tag="U4B62_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN2141304.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(1706..3118)
                     /locus_tag="U4B62_001132"
     CDS             complement(1706..3118)
                     /locus_tag="U4B62_001132"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN2141305.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3115..3651)
                     /gene="cysE"
                     /locus_tag="U4B62_001133"
     CDS             complement(3115..3651)
                     /gene="cysE"
                     /locus_tag="U4B62_001133"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN2141306.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(3737..3976)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3973..5433)
                     /locus_tag="U4B62_001134"
     CDS             complement(3973..5433)
                     /locus_tag="U4B62_001134"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN2141307.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(5603..6760)
                     /locus_tag="U4B62_001135"
     CDS             complement(5603..6760)
                     /locus_tag="U4B62_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN2141308.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(6794..8164)
                     /gene="radA"
                     /locus_tag="U4B62_001136"
     CDS             complement(6794..8164)
                     /gene="radA"
                     /locus_tag="U4B62_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN2141309.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8227..8754)
                     /locus_tag="U4B62_001137"
     CDS             complement(8227..8754)
                     /locus_tag="U4B62_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN2141310.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            8984..9790
                     /gene="proB"
                     /locus_tag="U4B62_001138"
     CDS             8984..9790
                     /gene="proB"
                     /locus_tag="U4B62_001138"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN2141311.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            9787..11034
                     /locus_tag="U4B62_001139"
     CDS             9787..11034
                     /locus_tag="U4B62_001139"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN2141312.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11110..11454
                     /locus_tag="U4B62_001140"
     CDS             11110..11454
                     /locus_tag="U4B62_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141313.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(11571..11897)
                     /locus_tag="U4B62_001141"
     CDS             complement(11571..11897)
                     /locus_tag="U4B62_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141314.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(12512..13714)
                     /locus_tag="U4B62_001142"
     CDS             complement(12512..13714)
                     /locus_tag="U4B62_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2141315.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            13831..14346
                     /locus_tag="U4B62_001143"
     CDS             13831..14346
                     /locus_tag="U4B62_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2141316.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(14545..14991)
                     /locus_tag="U4B62_001144"
     CDS             complement(14545..14991)
                     /locus_tag="U4B62_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN2141317.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15038..16516)
                     /gene="msr(C)"
                     /locus_tag="U4B62_001145"
     CDS             complement(15038..16516)
                     /gene="msr(C)"
                     /locus_tag="U4B62_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2141318.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(16870..17499)
                     /locus_tag="U4B62_001146"
     CDS             complement(16870..17499)
                     /locus_tag="U4B62_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2141319.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(17483..17947)
                     /locus_tag="U4B62_001147"
     CDS             complement(17483..17947)
                     /locus_tag="U4B62_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2141320.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(18434..19702)
                     /locus_tag="U4B62_001148"
     CDS             complement(18434..19702)
                     /locus_tag="U4B62_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2141321.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(19933..20670)
                     /locus_tag="U4B62_001149"
     CDS             complement(19933..20670)
                     /locus_tag="U4B62_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2141322.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(20737..21306)
                     /locus_tag="U4B62_001150"
     CDS             complement(20737..21306)
                     /locus_tag="U4B62_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN2141323.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            21434..22306
                     /locus_tag="U4B62_001151"
     CDS             21434..22306
                     /locus_tag="U4B62_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2141324.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            22648..23520
                     /locus_tag="U4B62_001152"
     CDS             22648..23520
                     /locus_tag="U4B62_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2141325.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            23517..25475
                     /locus_tag="U4B62_001153"
     CDS             23517..25475
                     /locus_tag="U4B62_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2141326.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
BASE COUNT         7326 a         5454 c         4781 g         8195 t
ORIGIN      
        1 tacgtcggaa atcaataaaa tgcatatctt tattttcttg ggctattgtt ttttccgttt
       61 tttttatcgt cttatataat tcataagcag aagttcgtaa tgctccttgg gaaaagatca
      121 tccactgttc tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct
      181 gaccagcgat tctctcgata taactgtcag ccgtttcgtc ttctttcgta aaaacaacat
      241 ccaactggta tttcttataa ttttgttgga tgcccggaac tagttgggca tcgaagacaa
      301 cgatgacttc cattccttca tatttcgcat aatttgataa acgatgtaaa agagcttctc
      361 ttgcatccgc cattttgtcc tgctttttaa gctggacaag ttccggccac gaaccaatca
      421 tgttgtagcc gtctacaatc aacagctgtt ttttcatggt ctactccccc tgacgttggg
      481 aaaaagcctg atacataagc aaaccagcag caacactggc atttaagctc tggacgtgcc
      541 cggtcatcgg gatcgtcagc aattcatcca tttctttttt caatcctgga ctcattcctt
      601 tgccttcatt accaatgact aaagcaattg atcctttggc attccatttt tggaagtttg
      661 tgccggtcat atctgtgcca aatacccaaa atcctgtttc tttgagctgt tgcacagctt
      721 gtaccaaatt cgtcacgcgt gcaacaggga catgttcgac tgccccagtg gaagtctttg
      781 ttacgatagg tgtgattcca actgctcggt gttttggtat gatcacacca tcgacgccac
      841 ttgcgtctgc tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa
      901 tcaaaaagaa ggggtcttct tttttctctt ttgttcgttc aatcagttgg tcaagggata
      961 aatattcata aggtgtaatc gcaagaacga ctccttgatg cacgccgtga tcgctcaaag
     1021 tttctaattt ttgtttagga acccatttga ctggcacagc ttgttcacgt gcgatttctt
     1081 tcagttcttc tactttttct cctcgtgaat cttcttgaag aaagagttta ttccctcgcc
     1141 cttgttgtag cgcttctact gcggcatggt gcccaaagac gaaattatct tctaatgctt
     1201 cttcattttg atcagaaact gactgatttc gtccagtttg ctttttattt gaatatctat
     1261 tttgtttttt gtcttcaaat ccacgtttcg attgatgttt gtttgttctt tctttaccca
     1321 tttttttcac caactttctg aatacaccag cggatcaatt cttccatccg ctcggtttgt
     1381 tttgtcaaat gcagatagcc catgagcgct tcgaaccctg ttgcgacccg atacgtcgta
     1441 acatcagcat tttttgcgct cgtatggctt ttagcatttc ttcctctgcg ataataaagt
     1501 tcttcttctt ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca
     1561 ttcgcagaaa cataattcgt tgccgcacga tgcagcttat tgggttttgt ttctccttga
     1621 tcaatcaggt ggtcacgaac atagatttcg taaatcgcat ctccaacata agccaaggct
     1681 aaaccgttca attgtgtgta atctctcatg cttctcttct ccatcgggtt ccttgcggtg
     1741 tatcttctaa taagatccct ttttctttca acaaatcacg aatttcgtcg cttcggacaa
     1801 aatcacgatt gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta
     1861 ataactcttc tgctgtaaat aaaataccga agatcgaaag ccattgcgag aataaagctt
     1921 ctccttgtgc caagacagcg gcagaaactt tcggctggtc tagataagtg ttcagccact
     1981 tagcaagctc atagaccact gtgatcccat ttgctgcatt aaaatcatcg tccatttctt
     2041 cgatgaatcg ctgttctaaa ttttctaaca aagcgatttt atccgcatca tctgccaaat
     2101 tctctgaggc agtttcttgg cggaatttca aattgtcaaa cgtattcttc agtcgttgta
     2161 aattggtggc tgcttctttc atcgtcgttt cactgtagcg gatcggacga cggtattgtg
     2221 tggtcgccat gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt tcatgtacag
     2281 tgatgaaatt ccctagagat ttactcattt tttcatcgtc ttcgccaatc gttacatagc
     2341 cattgtgcat ccagtaatta gcgaattttt tccctgtttt cgcttcactt tgtgcgattt
     2401 cattttcatg atgaggaaat tctaaatctt gcccgccacc atggatatcg atcgtttctc
     2461 ctaaatgttt tgttgccata acggaacatt caatatgcca accgggacgt cctttgcccc
     2521 atggtgaatc ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg
     2581 ggtcttcttt ttgctgctgt tcttcccctg tacgctgact tgctccgact tccagttcat
     2641 cgatggattg gtcacttaat ttcccataat tttcaaattt acgggtacgg taatacacat
     2701 cacctgaaga ttcataagca tagccttttt caaccaacac ttgaataaat gctaaaatat
     2761 ctggcatatg atccatgact cttggatgca gtgtagcagg ttcgacattc aacgctttcg
     2821 tatcttcttc aaatgctcgg atgaaacgat cagcaacttc tggtgctgta atacctaatt
     2881 cgtttgctgt acggatgatt ttatcgtcta catccgtgaa attcgatacg aaatcgactt
     2941 tatagcctcg atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat
     3001 ttccgatatg gatataatta tacacagtcg gtccgcagac atacataccg acatgtccct
     3061 tttgtatagg gatgaattct tctttttttc tggtcaatgt attgtagatt tttatcattc
     3121 tgatctcctc actttttctc ctttgattcg aacgactttt gctggtactc ctaccgctgt
     3181 agcgtcgtct ggaacatctt tcaacacaac ggcacctgca ccgattttgg cgttttttcc
     3241 gatcgttacc ggtcctaata tctgcgcatt cgcagagagc atggctcctt gtttaacagt
     3301 tgggtgtctt ttacccgttt cttttcctgt gccgcctaat gttacgccat ggaataaaat
     3361 cacatcgtcc tcgatttctg cggtttcgcc aatgacgatt cccattccat ggtcgatgaa
     3421 cacgccagtt ccaatcgttg ctcctggatg gatctcaaca ccagttagga aacgccagaa
     3481 ttgagcattg atcttcgcca ataaaaagag ccggtgctga tataaaaaat gagagaaacg
     3541 atgccaaaac aaggcatgaa aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc
     3601 agccggatcg tttctctttg ctgcatcaat cgctcgggta aaccaactca tgactttcca
     3661 ctcctttcgt aagaatttta tgtaggaaat catcttctta taaagaaaag aagcgccttt
     3721 taaataagtt tccttatcaa aagacgcttt ccgcgtggtt ccacttttct tcataatcaa
     3781 tgattatctc tgacagctaa cgcctgttat acgttcttgg ctactttgtt tcctataaaa
     3841 cagtttttcc tgttttctgt gtttcgccaa gaccactccc agaggcattt cggcaaccat
     3901 tcattgatac cttgcaccac ccggtatctc tcttgtaatg atcagctgct tacttttttc
     3961 tgttcatcgt gtttagttca gtacttgatt taaatgtgct aatgattttt cacgaccaag
     4021 caattcgatc gtttcaggca attctggacc atgcatttgt cctgatacag ctacacggat
     4081 tggcatgaaa agattttttc cttttacgcc agtttctttt tgaacagctt tgatggcagc
     4141 tttgacagtt ggagcatcta ctacttccat ttgttctagc tgtgctttga atgcgtggag
     4201 aacagtagga actgtttcac cagcaagtac ttcttttgct gcttcattca atacaggatg
     4261 ttcattgaaa aatagcgtag acagatcaac gatttctgca gcatagctca tttgaggttg
     4321 atacaggcta acgatcttct tcacccattc gattgtcttt tcatctgggt tttctgctac
     4381 acgtccatca gcgattagat aaggcagaca catgtcagta aatgcatcta gatccattgc
     4441 tttgatgtaa tggttgttta cccattgcag ttttttcgca tcgaaagctg cgggtgattt
     4501 acttaggcgt tttggatcga acattttgat caattcttgg tgttcgaaga tttcgtcttc
     4561 gcccacaggt gaccagccca ataatgcaat aaagttgaac attgcatctg gtagatagcc
     4621 taattcgcga tattgttcga taaattgcaa gattgattcg tcacgtttgc ttagtttttt
     4681 acctgtttca ctgttgatga tcaatgtcat atgtccgaag gttggtggtg tccagccaaa
     4741 tgcttcatag atcatcagtt gttttggtgt attggcaata tggtcatctc cacgtaaaac
     4801 gtgagtgatt ttcatcaaat gatcatcgac cgcaacagca aaattataag ttggcatacc
     4861 atcgcgtttt tggatgacaa agtcgccacc gatattatcg gattcgaacg aaatagcgcc
     4921 tttcaccata tctgtaaatg cgtattctgt attgcgaggc acacggaaac gtacaacaga
     4981 ttctagacct tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc
     5041 tgcgtaatga ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc
     5101 ttctgtgcaa tagcatttgt atgctcgatt gctagcaagc agctgatcaa tcagcggttg
     5161 atagatttct ttacgttcag attgacggta agggccgtat tctccaggat tttcaggaga
     5221 ttcatcccaa ttcattccca accaagcaag gttttccaac tggctttttt cgccatcttc
     5281 gatattacgt ttttggtcag tatcctcaat acgaataata aagtctccat cattgtggcg
     5341 ggcaaacaaa taattgaata atgcagttct tgcatttcca atatgcaggt gcccagttgg
     5401 acttggtgca tagcgtacgc gtactttcgt cattttcttc ctcttctttc aaactagact
     5461 ttgactaaca gcaaaaattc cgtcagttac ggcttctcaa atatcattta tttaaagcca
     5521 ctcagcacaa attttacctt ttctaaggta aatagtaaag ccaactggtt gaatttcctt
     5581 accagttggc tgaatttctt ttttattttg attgtttagt ctttttctct gtctcttttt
     5641 tattgtcgat tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg
     5701 tttgaagggc acttgttaca acgacttcga tacgttcatt catgtaatgc tgtccatctt
     5761 ctacgacaac catcgtgccg tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg
     5821 cttttactac tagaacattc atcgtttcac cagggatgac gactggtttt acggcatttg
     5881 ccaacgcatt gatattaaga acaggtacat tttggaattc agagacttta ttcaagttat
     5941 agtcattggt tacaatgaca ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta
     6001 cttcactgat atcttcaaag tctccatcgt acatttcgac agatataccc tcttcttttt
     6061 gcaaggcatt taggatatct aatccgcgac gaccacgcac acgtttcaaa ctatcccctg
     6121 agtccgcgat gtattgtagt tcgtaaagaa caaaattagg gatcatcaag gttccttcta
     6181 agaatccagt ctttgtaata tcataaatcc gaccgtcaat gatcacgctt gtatccaaga
     6241 ttttatattt atggaaattt tctcctgatt ttcgttctaa cagttgactc tcaacttgac
     6301 tagctgtttc ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc cattcttcga
     6361 ttcgtgtcgt tcccattctc aatcccagat agcctaagat gatcatcagt aaaatcggca
     6421 atacactatt gacgaatgga atttccatat tgtaaagagg ggtcgagatc acaacaccta
     6481 aagtcaatcc gatgatcgca ccgatcgcac cgaagagaag ataagtcaag ctgacctcac
     6541 ttagtttttg ttctacttgt ttgataatag ctgagatatg tttggccaac agcaaagata
     6601 ataaaaagaa aataagtgcg cctaacaggc tgttggtaaa tgtattattc agccatccgt
     6661 ttgactgttg accgattgcc tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc
     6721 cgacaatgat catcagcaaa gtaatgacac gtttttgcat cgtttttcct ccgtttcgac
     6781 aatcatttat tagttagcgg aacacacgct tcaacgtttc agccaaagtt gctactccaa
     6841 ctatctcgat tccttttggc gcttcccagt tacctaagtt gtttttaggt aggtatactt
     6901 ttgtgaagcc gagtttttgg acttctttga ctcgttgttc aatatgactg acacgtctga
     6961 tttctccagt cagtccaatt tcaccgatga aacattccgt tggctgtgtt cctttttctt
     7021 tatagcttga agcaatactg acagcgatag ctaaatcaat cgctggttca tttaatttta
     7081 ccccacctgc agcttttaag taagcatctt gattctgaag aagcaaccca gctctttttt
     7141 ccagcactgc catgatcaga gatacccgat tgaaatcaag acctgttgtt gttcgtttgg
     7201 catttccgaa catagtaggt gtgactaatg cttggatctc taccaaaatt ggacgtgtcc
     7261 cttccattgc cacaacgata gcagagccag ttgcaccgtc taggcgttct tctaagaaaa
     7321 tctgtgaagg attagcgact tcttccaatc catgctcatg catttcaaaa atcccaatct
     7381 cattcgttga gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt
     7441 caccttcaaa atataacacc gtatccacca tatgttctaa cattcttggg ccggcgatcg
     7501 agccttcttt ggtaacatga ccgacgataa aaatggctat cccatttgtt ttagcggttt
     7561 tgagtagttc tgcagtggtt tctcttactt ggctgacact tcctgccaca cttgtgatgt
     7621 ctggctgcgt catcgtttgg atggaatcaa tgataacata atcaggggag atatgttcga
     7681 tggcacgact gatttcgttc atatctgttt cagcgtatag ataaaattct gtgttgatac
     7741 ttccgaggcg ttccgcgcgt aattttatct gctctgcgct ttcttcgccg gatacatata
     7801 agacttttcc gcctatggct gctaattgct gagaaacttg taacagcaaa gtggatttcc
     7861 cgatacctgg atcgccacca atcaagacca tagaccctgg caccacacct ccgcctaaca
     7921 cacggttcag ttcttctaat tttgttttga tccgaggttc ttttttaggg acgacgtctg
     7981 cgatttttgt tggttttgct ttttctcctg tcaaacttgt ccgagttctg cggtccgttg
     8041 tatcttgttc gatttcttcg accatcgtat tccattttcc gcaatttggg cagcgtccca
     8101 agaacttagg cgacacatac ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg
     8161 ccattgtacc gcctcctttt atcctaatca tgagacatta aatcaagctt aagtatcttt
     8221 aacgttttat ttactggaag aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg
     8281 atctgctttt aaaaacggca aaaagatccc ttggccgata cgctcgcctt ttttgatcac
     8341 tacatccttg aaaccaaagt tggtgaattg gaacatgata tggccttcat ttgcctcatt
     8401 attgtaataa tcactgtcaa tcacacctac tccgtttgtc aacactaaaa accgcttcaa
     8461 cggattgctt gaacggttag caagctgcaa aaattcatct tctcccatgt aagctttgat
     8521 tccagtaggc acgagggttg gtttcaattg tttttcattt tctgatacat cttttttcaa
     8581 tccatctgcg actgtttcca accccttttt ccagcctgct ttccagatac ttggaatcac
     8641 gacctcttct gctgcttcaa aatcatagcc agcagcatga tgtgtagcgc gctgcggtag
     8701 attgatattt tgatctttat atttagaaat aacttcaaat cctctttttc tcaccggttt
     8761 tcctccctaa ataaactact cctattctaa catatccgag gctgtaaaag actttcttta
     8821 caaattcttc aagaaattct gctatcttaa agtaaaccat taattcgttc tttttccaaa
     8881 cgactgcaag ttgtacaaaa aaactatttt ttacatgact ttactcgtca aaacaagtag
     8941 aatagaacga aattaatgag tggatatttg attgaggtgt cacatgcgta aagaaattaa
     9001 aaatgcaaaa cgaattattg tcaaagtagg aacaagtaca ttgatttatc ccaatggaaa
     9061 tatcaatctt gcggcaatcg atcagttagc ttttgtcctt tctgatctta gaaatcgggg
     9121 aaaggaagtg atcttagtct catcaggagc tgtaggcgtt ggtatgcata aattacattt
     9181 gtcagaacgt ccgactttga ttcctgaaca gcaagctgcg gcggctgttg gtcaagccga
     9241 actgatgaat atctataatc aaagattcag tgtatataat caacagacgg cacagatcct
     9301 cgtgacaaga gacgtggtag attatcctga aagtcgtaaa aatgtgagga atacattgga
     9361 acagcttttg attatggaca cgatccctat cgtgaacgaa aacgatactg tagcagtgga
     9421 tgaattagat catctgacaa aatttggcga taatgatcag ctgtcagcca tcatggcaaa
     9481 acttgtcgcg gcagaccttt tgatcgtcct ttctgatatc gacgggtttt attcagataa
     9541 tccgactaca aatcccgagg ctcagatgta tcgctatatc gataaaatcg atgaaaagat
     9601 tctggcacaa gcaggtggtg ctggtagcac atttggaaca ggcggtatgc agagtaaatt
     9661 aaaagccgct tctaggatat ttgaagcaaa cagcgcaatg attttagcaa atggaaaaaa
     9721 tccggcaatt atttttgata ttcttgcagg aaaagaagta ggtactttat ttaaggagga
     9781 cttgaaatga ctgacttgat gcaattagga caacaggcaa aagatagcgc gcaacaacta
     9841 gcacttatgc ctacaaaccg caaaaatgag ctacttgttc aaatggcaca aaccatcaaa
     9901 aagaatcaac aagcgatcat tgaagcaaat cataaagact tagaaaaagc agtcgaaaac
     9961 aatatcagtg aaacgatgca agaccgcttg cagctgacac ctgagcgaat caatgccatg
    10021 gctacagaga tcgaaaaaat tgcttcgtta gacgatccta ttggaaaagt agatgagatg
    10081 tggacgaaca ccgacggatt gagaatcggc aaaaagcgcg tgccactagg cgtgatcggc
    10141 atcatttacg aatctcgtcc aaatgtcacg acagatgcag ctagtctagc attcaaatca
    10201 gggaatgccg ttatccttcg cggtggcaaa gaagcattct tctcgaatca attattagtc
    10261 caattattgc agcaagtact ggtcagtgct ggcgaatctc cttatgccat ccagtttgtc
    10321 gatgatactt ctcatgaaac tgcaggacag ttgatgcgat tgacagaata cttagatgtg
    10381 ctgattcctc gaggcggagc aaaattaatc caacgagtca aagaacaggc aacggtacct
    10441 atcattgaaa caggtacagg aaataatcac gtatatatcg ataaagaagc acagttatct
    10501 atggcagtga acatcatcgt caatgcaaaa gcttcccgtc catctgtctg taacgcggca
    10561 gaaacattgc tgatccattc tgagatcgca ccttttttct tgccagctat tgaaaaagaa
    10621 ctggtagaac acggggtctc tttacgtgcg gatgctcgtg cgttagaata tctagagacg
    10681 gctgctttag cagaagacac agattgggat actgaatatc tagattatat ccttgctgtc
    10741 aaagtggtcg attctttgga tgaagcaatt gcacatatca atcggcataa tactaaacat
    10801 tcagaaacga tcgtgacaga tagttatgcg gctagtcaac gtttcttaaa tgaagtggac
    10861 gcggcggctg tctatgtcaa tgcctccact cgttttacag acggatcggt atttggtttt
    10921 ggtgcagaga ttggtatctc tactcaaaag ttgcatgccc gtggtccaat ggggttaaat
    10981 gaattaactt ccacaaaata tatcatttat ggcgatggac aaatccgcga ataatgtttc
    11041 gtgtgaaaca ttattcccgc cttttgcctt ttttctgtaa aggttctatc caactagaaa
    11101 ggtgtcaata tgtttgaaat tttaatgatt ttttttattt accttatttc tctgaatatc
    11161 gctgcttttc tgggagtcgg tattttatcc cttttctttc aattcaaaaa acgaagcata
    11221 gggagtcaac gggaaaaatg gtcgcagtat tttgacaaaa tcgggcaaaa agggctggtt
    11281 acacgcttgc acattagtta catggttgca ttatgcttat tagcgacaat caattactat
    11341 tcttttttcg atcattcaat cgcttatacg attacgctac tgattgctgg gatttttcac
    11401 ttatcttata aatatcagct gaataaaaac cacttgaatc ggactttccg ctaaaaaata
    11461 tccgtacgat aagtagagtg ccttgaagat cggcaaaaaa ctgccggact ttttagaggc
    11521 tgtgacaaag attttgtcac agcctctttt tccatataaa cggcgttcgc ctaccagctg
    11581 cctggtaaat ctgtttcctc ttcgttaaga agcgaaaatc cgtcaaatgt ttcgccatca
    11641 taataacgaa gcagctcatc catggacaag ttcatcgttt tttctttttc ttcttccgct
    11701 gattcagcca gtagctgttc ccgatacacc tgatacatac cagctatttt ttgctgcatc
    11761 gcttttctag tttcaccttc cgcattgatt attggcaatt ctttgatata gccacgataa
    11821 atcaaccgat catctgaaaa ttcaatgggt aattcaacga gtgtcaaccc gacttcctga
    11881 aaatattctt ccagcatcta ttatcccccc attctttttc ttaaatgaat tttagcttac
    11941 ctactgaaaa ttttccagtt tcattatttt gagaaaaaaa gggaatgtga caaaaatcgt
    12001 gtcacatccc ctttttccga ataaatggtg ttcttctgcc aactcctcaa tcataagtca
    12061 caatattcaa caaacatgag aaactgtttt ttgaaattgt tccttgtgat tcagagttaa
    12121 acgacagtct cacaacctct tggtaaacgg cgttcaatca gctgaccctc ggtattagct
    12181 caaaaaacgg taaaatatga gaagctattt ttccgttttg tttctctaat acttaggata
    12241 aaacgctgat tttacaacct ctgttaaacg gtgttcaatc agctgtcctt cggtattagc
    12301 tcaaaaaacg gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggaca
    12361 aaacgctgat tttacaacct cctgataaac ggtgttcaaa agcttgcatc tgcggtaata
    12421 agcccaacca aacgaaaaat atgagaagct atttttgttt ggctgttctt atgacttgat
    12481 gcaggacagc tttttcacaa cctctgattt cttattcttc ttcatttgta gcaaaataat
    12541 tacggtgata aatgatacaa catacactga taagaacaat ggcacctgca aaataaaacg
    12601 catagctttc accaatatgt tcaccaatca cacctgcaat aaatggtgct aaagctccac
    12661 cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt tcgttgcaga cctggtattt
    12721 ccatagcaat cgtcgtcaac agtgtattga taattccttg gaaaaatcct gctaacacaa
    12781 tgctgatcgg tacaaggact ggcatagaag ttccagctcc taataacagc aaacaaataa
    12841 tgaataagac aaatgctgct aaaattgtcc gataagtcgt aaacttccgc tcaagagccg
    12901 gtgcaacgaa aatcgaagca atagccagaa gcacacccca gccaaaaaat acaaaaccga
    12961 cttctaattc agaatagcca atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc
    13021 cgaaattata taacaatgcg attagtccaa ctgaacgaag tttatgattg cttaacgctt
    13081 tgacaccttc ccaaaggcct acttttctct ttggtttctc agtttcaggt aaaagtaccg
    13141 ctagggctat aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga taacgccatg
    13201 aaccactgcc caagacccca ccgactagag ggccaacagc cattccgcag ccaagtgctg
    13261 cttcatacat catgattgct ttttctgtgt taccaactaa aatactgacg atcgttgcta
    13321 aagcagtaga gacaaataaa gcattcccga caccccagcc ggcacgtaac ccaatcagca
    13381 ttccgatatt cccagataat ccgccaagac cagcgaatac aacgatgata aacaatcctg
    13441 tcatcaatgt ttttttcaaa ccgatccgac tagataagaa acctgtaaat agcatcacca
    13501 caccagtgac taacatgtaa ctagtaaata acagggtcgt ttcagctggc gtagcgttta
    13561 actgtgcagc aatcgttttt aagattgggt ctactagacc cactcccata aaagcgatca
    13621 tacaggtagc tgctacaact agcgctgaac ttttcccctt tgtttcttca tttgtttgtt
    13681 cttgtatatc tatttgattc tccaatattt ccatcccttt ctctctatta gcatcataca
    13741 tgatttattt tacatgtcaa tatgcacatg taaaacgtgg aaactttact gtgatataat
    13801 aaagaaaata agacgagagg taggattttt atgaatactt tagcctatat attattatgc
    13861 gtgcttttat ccaaaccacg ttctgggtat gaattgaagc agctgatcac aattttttgg
    13921 gaagcccatc acagtcagat ctatacaacg cttgccaagt tagaccagca gggttatgtg
    13981 accgttttgg acaaagacga gcactctcaa aaaaagatct accacttgac tgatgaagga
    14041 cgtgctcttg tagaggaatg gatcaaagag gaaactcccg ctcctacgca aaaagatgaa
    14101 tttctagcaa aaatctatgc tttttcgacg ctcgataaaa caacagctac tggactgcta
    14161 ttcactagag aacaacagct gaacaaaatc atgcaaaaaa atcaaaaaaa attgaatgac
    14221 ctctcgctag acaagaaaca agaatttgga cgttatgttg tgatccagcg cagaattatg
    14281 ctttgtcagc aggaactcca atggtgtcag caagtaagag aacaaatgca gcagtttttt
    14341 gagtaaggga taaacagaac ctttctagaa atatctattg aagagcaaat tatcttcaat
    14401 aaaagagcaa tacagttgga aaagaaaaag cgatccgaac gacaggccaa aacctatcgt
    14461 tcggatcact atttttctag aactgcattc aagagactat gacaaaaagc atgccaaggc
    14521 cttttttcaa ataacagtgt tccatcattc attactttga attgctttta gcacacttct
    14581 tgaaagcggg ccaacgatga tcaactgcaa cggtaaagcg gcaatcacat tcattttcca
    14641 agcattcata taagcagaga ataattgatc aggtattcct tgttccatga tcacaccgaa
    14701 taaagacata catgtcacca ttcctaaaac catcaagcat gaaatagtta agatcatttg
    14761 gaccttgctt tctggattta ctggtagttt aaaggcaatt tttttcgcca gcacaccgat
    14821 gatgaagaca tccaaaatga aagccaccac gaatccaggc actagaccaa tcaacaaact
    14881 tgataaatga agatcttgat gcagccataa attatacgcg ctcatcccca agaccatcaa
    14941 aaaacacatc attgttgtaa aaattattcc ttctttttta tttgtaggca atctgtacca
    15001 ctcctttaaa tttgtcacga aaatcatcgt agcacattta aaaatttctc gtaagtactt
    15061 tttttatttt ctgccctttt atttcataaa cttcatcagc cgtacgctcg acaaagtaag
    15121 aatcatgaga agtaaacaaa atcgttcccg gatatatttg cattagcttc tctaaagctt
    15181 cgatcgttgc catatcaata aaattagtcg gttcatctaa cagcaacaca ttacttggct
    15241 tagtaaaaag caaagcaatc gtcagacggg tcgcttctcc cccacttaat gtacataacg
    15301 gtttcgtcaa agtttcctga gcaaacccta agtgattcag caaactacgg acaagcgatt
    15361 ctgaaaactc tgtttgtctc atgacaaaac gaatgatgga ttcttcagat gtcatttgat
    15421 aacccttttg ctgatatact tgaaagctta ctttcggaga gagaaggatt ccttctcctt
    15481 gtttgcggat atgttctaat aaggttgtct tacctgaacc attttcaccg acaatcgcga
    15541 tttttttacc taaagaaaac tgaaaatcac catttaccag taatgttctc ccacttcttt
    15601 ccaattggac attttgtccc atgatgggat agcggctgtg gagttcaaga gattttggta
    15661 ctggaaaacg gatttgtttc atctgctgcg gcttggttgt ttctcctatt ttttggagtc
    15721 ttctctctaa tgttttcgca ttcttttgga tggctttttg tacactgtct ttttgtttag
    15781 aggaagacaa ccgatccagt ctgatttgtt gctttttctt tttactggac actttttcca
    15841 acttttccgc ttgttttttc ttttcttgga tcgatttttc caatcgtttt ttctctttca
    15901 tgatcgactc tgcttctcgt agctgagtct ttttctccaa ttccttttga gtgagatagg
    15961 cactataatt ccccgaaaac tctcggattt ctccgtcttt tacttcccag attttcgatg
    16021 ctagctcatc tagaaaatag cgatcatgac taacaaaaac cagcgtacca taataataac
    16081 gaagctcttc aatcagtcga tcaatacttt ttctatctag atgggttgtt ggctcgtcta
    16141 gaagcatccc ttgttcataa gaagaaagaa gacgtatcaa ctcgatcttt cgttcttccc
    16201 ctccgctcca tttccgctct gaagtttctt ccagtcttaa ctggaaatga ctggctaatt
    16261 ccaaatttag gtcctttgct tcagcgagat aggttaattg aggcaggtag ttgaaggtga
    16321 tctctgtttg gattttccca tgatcgggaa aaagttcacc ggctattagt ttgagcaaag
    16381 tcgatttacc gacaccattt tctcctatga tccctatacg atcttgctga tagacacgaa
    16441 gagaatcaat agatagttct agctggttgc caaagctgac ttgtagattt gttatattta
    16501 ctgctaaatt ttccatatgt tttacactcc tatttcatgt aaaacaacaa ttatcgatac
    16561 taaaaaagag cagaaaaatc tgtttgcaaa aaaactgcat cacatctttt cctactctga
    16621 acgaaataac tatgactaaa tcaccaaatc tgtcatagct ttcacctatt ctactgtcgt
    16681 tcgaaaaaat ggacatactc catccatgaa attgttgttt atacatgtct tttgaattta
    16741 tttagttgtt attcaaaagt tcgaaacgta atttcatcga tgcagtcatt tttccgacct
    16801 ccttttttat taggttacca cacacgattc aaaaaaagca atagctactg aatagtaact
    16861 gaaaaccttt tattcgaaca tagtgatctt ttctggtgct aaacgaacag actcgtaacc
    16921 ttcttctacc gcttttccta ttgataaaat aataactgga acataacggt ctttatccaa
    16981 accaaatgcc tctgccaact gatctgcttc gaatccacca attggatttg tttcatagcc
    17041 atgatcacgc gcaactaaca tgaattgcat agccgctaag ctagaatcga ctttgactac
    17101 atcgttcatt tcttctcttg aaaaactctt gtaataagga atgatcgcag ctaattgttg
    17161 atctctgact tcttttggca tcttccctga ttcgtaagct tggttgtaga tttcttcgcc
    17221 atattcgtaa cattgcatat cgccaaaaat catgaccatt gcagaagaag tatcattttg
    17281 tctcgtattg aaacggatca aaggacgcaa gatttctttt gcttcctcgc tttcagcgac
    17341 cacaaagcgc catggtttca tattgacaga agaaggtgca gtcgtcgctt ctttgatcat
    17401 ttccagcatt tcttcgtgtg ggattttgta tgtttcatca tatacgcgaa cggatcttct
    17461 accagtcata attgttgaaa aatcattttt ttgttccatt attattaacc tctttcgttt
    17521 ctgttgttat attgcaaagt tgggtattca atttatctaa aagtgttaga aattgtttca
    17581 tttctacatc gctgaaagcc ggggatagca cggaacctgt atctttatgg tccatttcac
    17641 aatgcttgat tttcttctgg gctttttctg ttagcgctac atacacttcc cggttgttct
    17701 gaggatttct ctgacgcgaa acatagccat ttgcttccaa taccttcaga tgacgagtaa
    17761 ctgctccttg atcaatctgc aacttttctt gcaaactgct ttgtagcaat ggattttctt
    17821 ctttcagtct catcatgatc tcgtatctag taagacttat accgatattt tcttcaaaga
    17881 tctggacgat tttttgatcg gctaatttga tttgatataa aaggtgacta atctcttcta
    17941 aagacataat ttcctccttt ctaataattg actcgtcaac gattgacttg tcaataatag
    18001 aataaaaaaa gaaaaaagtc aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga
    18061 aaagtataga gaaaagaaat tttttgtaga atgaacggaa aaaatgataa agaaagcaca
    18121 acgctaaaac caagattatt gattcttatc aaactataga agatagctct tctgcagtag
    18181 ataattttac accgccaaaa atttccgaaa tcgccttttg caagaatccc gtcgaatatt
    18241 agaaattgga tcagtatgct taaacagtaa taaaaaagtt atgacaagat gttgtcacaa
    18301 ccttttttga gtaaacggtg ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca
    18361 aaaaacatga aaatctgttt ttgtttggct gttcttacta cttgatgcag ggcagctttt
    18421 tcactacctc tttttaataa ccataaaatc ctattccttc atatctccag cccatcggtg
    18481 ttagccaatc tctttcctta gcactcaagg tatagtgatg ggtaccagtt ttttcatttg
    18541 gattaaatag tcgataaatt ggcttggaac caccagaata aaaacttaga ccttcataac
    18601 gccaaccgat ttttgttaaa gagttgatct cagattgtgc cattgtgtaa tgatggtctc
    18661 cactgtaagg attgtataaa cggtagacag gttgtccgct gtttggtgct tgccaagcaa
    18721 tcccttcata ttcccagcct acagttggca gataattttt ttctccggca ttcaatgtat
    18781 ataaatgttc tcctgtatga cggttataca gacgatacat cggagaagtt gaagttggtg
    18841 ctgtatcata ctgtgtcaga ttataggttt cgattacatt gttcaatgta gaaccataat
    18901 taggcgcagt ggcataacga cctgttagcc aagccgtagc atctttgtaa gagttcgtat
    18961 tgcttttcca tgcaccggaa tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata
    19021 ccgttacgtt atcttgcaat gattctttgt acgaaggata ttttctaaaa gcggcattca
    19081 ccgtaatcca ttgcccattc acatattccg aagtaggcat cgtaacagat tggccattat
    19141 agcttccttt aatcccaaat agattgtaat taggggcaga agccaaagta ctgttccccc
    19201 agccactttc aacgatggct tgagcaatca tgactgatgc atataagtca ttggcacttg
    19261 ccaaactttg tgcagttgag gcaatactat caatgaacgc ttgttgagaa ttagcagatg
    19321 agcgagcata aaaagcatag ccagtatagt tttcgaattc agacaaatct gcttgatggg
    19381 tatgtgtcaa tgtttgccca tctgtcaatt ggttcgtgat ttcttctgca ttctctggat
    19441 tgttctgagc ttctgctgat gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg
    19501 ttggttcttc ggttgaaatt gtattttctg agacagtagc tgactgcaaa gtactttctg
    19561 tatcgttttc aattcctgtt tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg
    19621 attcttcagc tagcactgtt gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa
    19681 ataataaatt ctttgctttc atcttttttc tcctttgaat tatgaaactt ttcctaaata
    19741 acagtgtaac taaaaaagta aaagcaagtc aatgtccata tttaaataac atttgtttct
    19801 ttttaataaa tttgcttttt ctgcataaaa agacgtaaat gaagaatatt cttctccatt
    19861 cacgccttaa attgatggtc gtttgaataa ctatttaaca agtaattctc tctggatatt
    19921 atccgttatg gattatactt cctgtgtcgt cggacggata atcatgttgc tgacagacat
    19981 ccggtctggc gtttctatcg caaatgctac agcagaagct atatcttcag atgttaatcc
    20041 ccattctttt tgcgcttcat gaagttcaag agcagccttt ttgtcgctga tggtttgata
    20101 taattcagtt tgaacagctc ctggagagat gatcgtagat ttgatgttat tttgccgttg
    20161 ctcttgacgt aatccttcca tgattgcacg gacagcaaat tttgttccgc aataaacggc
    20221 ggaatcagga taaacaacat gtcccgccac agaatcagtt gaaatgatat gcccagattt
    20281 ttgtttttcc atgattggaa gtacggcgga aattccattt aacacaccca tgatgttgat
    20341 atcaagcata ttttgccatt catcacggtg accttcaact aatggagcgg ttggcatgat
    20401 tcctgcatta ttgtacaata catcgattcg accgaacttt tccatcgcta aatcgataac
    20461 tgcttggact tgtgcaaaat ctcttacatc tgctgtttga atatatagct cagcttctgg
    20521 taaagattct tttattgctt ttaggcgatc ttctcggcgt gcagctataa ctagtttagc
    20581 ccctttttct gctaatagct tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat
    20641 gacaatcact ttttctgata aatcattcat tctattctcc tcctgttttg aaaaatctct
    20701 tgcttatact cactatctct tatttcatct tactgttcaa ataatccgtt cgacaggttt
    20761 gacgattttg gcagggattc ctgcaacgat ggtattgtct gggacatctt tcgtaacagt
    20821 tgcattcgct gcaacaatgg cattctcgcc aatcgttacg cctggcagaa tagttgctcc
    20881 tgctccgatc caagcatttt tcttgatgtg gattggcttt actgacaagc ctcttcgttc
    20941 ttttggatcg gttagatggt tcaccgtgat gaggttgaca cgaggaccaa tcaatactga
    21001 atcttctaaa cagatgccac ctaagtcaac gaacatcact tgacgattga taaagacatt
    21061 tttccctata ctaatatgtt tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt
    21121 atcatctact ttgtcactaa tgatattttc tagccgtttg cggatatctg ctggttcatg
    21181 gtacccagta ttcagcgctg ctaaaggctc ttcgttttcc gcttggatct gatggatcac
    21241 ttcaaagagc ggatccgtta ctcgaatctc tttattgacg atttgttttt cgatgaggct
    21301 tgtcattttg ctccctcctt ttatccttac tttataacgg acggcttatg atgtctaata
    21361 cttataaaac atacttaact attcctttta actatactta gtggtatgct tttttaaaaa
    21421 caggagggaa aaaatggata ttcgtatact gacgtatttt ttagcagtag ctagagaaaa
    21481 aaatatcagc cgagcagcaa aatctctgca tatcacgcaa cctactctct caaaacaact
    21541 gaaagattta gaagaagaac ttggtgtgac acttttcact cgaggctccc gagagataca
    21601 gttgacggat tctggtcgat atctttattc aaaaggaaaa gaaatcttgt cgttggtaga
    21661 aaaaacggcg aacaatttaa caaatgacgc aatcatctct ggagaaatct acattggcgg
    21721 aggcgaaact aaagcaatct ctttcatttc tgatagcctt cattcgttga tcaaggcgca
    21781 tcctgatatc cagtttcatt tatatagcgg gaatgcagat gatatcatag acaaactgga
    21841 tagcggatta cttgattttg gactcgtaat cgaaccgaca aacaaacaaa aataccagtc
    21901 atttcgactg ccagcccaag atcgttgggg acttttgata catcggaatc atcctttagc
    21961 aaagcaaaca gtcatccatc cgcaggaact gaaaactgtt cctttgatca tttcccggca
    22021 aacgtcagtg gacagtcaac tagctgaatg gctaggtttc agcatagatc agttatcgat
    22081 cgtcggaaca tataacttat tgtacaatgc ttctttgatg gtcgataacg ggacttttgg
    22141 tgcattagct ctagatggga tcatcaatac ttatgggacg gaaaaagtat tcatcccact
    22201 cgatccgcca ttacaagctt cactgaatct catttggaaa aaagaccagc ctctttctcc
    22261 tgctgctcgt aaatttttag atgtcctaac ggagaaatac agttaagtag agtttttttc
    22321 cttcgctttc agctttttaa caacgaataa gttaagcaaa agatccaatg attaaataac
    22381 ataagtttct taatttgttc cttcctttta tttctgataa ttccagtaaa ttactataat
    22441 tataaaaaaa gaattgacaa aacataactg tctatataaa attgattacg ttaacaataa
    22501 ttcgtttcat gtgactagat catactaggc atgaaaaatc tctgaggaac atggtttccc
    22561 taggaggttt ttcgtgtcta tttttgtttg gccgcaatca actggtctag caaacgtgaa
    22621 aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca
    22681 gtattggtag atgacggtgg acaggaaaaa gtagctattg gaaaagggat tggatttgac
    22741 agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac ggatatttgt catggaacca
    22801 gaaggtcaaa tcaagttgca aaatttactt aatcagattg atgaacgctt tttatttgca
    22861 gcagaaacga tcattgatca tgcggaaact gttttgatgg aaaagttgaa tgaacatgta
    22921 ttgatcgctt tgaccgacca cattgctttt tctgcggaga acattaataa cggtatcgtc
    22981 atccgcaaca aacttttaag agaaatcgaa gtgctttaca gtgatgaatt cagtattgct
    23041 caatgggcag tagagtatct gacaaaagag cttgggattc catatactta tgacgaagca
    23101 ggatatatcg caatccatat tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt
    23161 atccgtgaag tgacgattgt atctgacatc atccatttag tggaatcaga attggaaaca
    23221 gatatccatt cagaaccatt ttcactgaat tattcaagac tggtgaatca tttacgactg
    23281 cttctacaac gaacccatgc gcaacaatat gctgttttag acacggaaat cgtagaaatg
    23341 gtcaaacgaa aatacccaga aagctataag atttctaaaa aaatccgtgt gttgctgacc
    23401 aaagaatacc agctcgcgat cacgaaagaa gaactgggtt acttggccat ccatatcgag
    23461 cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc atgaggagga aaaaaattga
    23521 aagcatatct acaacgaatg ggacgttccc ttcaacttcc agttgcggtc ttacctgctg
    23581 ctgctttact agtagggatc ggaaactggt gggcatctta tagtaacgac attgtcgctc
    23641 attttttaca agctggcggt aacgcagttt taggtcaatt acctttgctg tttgccgttg
    23701 ggttagcact agggatgtca gtggacaaat caggtgctgc tgccttggca ggtttggtcg
    23761 catttgaagt tccagtaaat gttttaaaaa ctgattcagt agctacttta ttgaatatca
    23821 aaccagaatc agttgatcca tcttttgctc agatcagtaa cgtatttatt ggtattattg
    23881 ccggattgat tgcggcagct ctttataatc gtttccacga agcaaaatta ccgatggctt
    23941 tatctttctt tagcggcaag cgtctagtgc caatcttggc tgcctttgtc atgttgctga
    24001 tcagtgcagt gctactgctc gtatggcctc cggtttactc tgctttagtt tcttttgcta
    24061 aattcatttt gagtttaggc tggggtggtg caggtcttta tggcttcttc aaccgtttac
    24121 tgatccctac aggattgcat caagcaatga actccgtgtt ctggtttgac gtagcaggaa
    24181 tcaatgatat tggtaaattt ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc
    24241 aagctggatt tttcccaatc atgatgttcg ggcttccagc aggagcatat gctatttaca
    24301 gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt gatgttagca gcagcttttg
    24361 cttcattctt tactggtgta acagaaccac ttgaattctc atttatgttc gttgcttggc
    24421 cattatatgt cttgcatgca attttcactg ggatttcttt agcagtgagt gcattcttcc
    24481 attggacagc aggatttgcc tttagtgccg gatttgtcga ttacttccta tctttgaaaa
    24541 atccgattgc gaatcagcca ttgatgttga tcgtccaagg attagtaaca gcagtgattt
    24601 actactttgg ctttaacttt gctatcaaaa aattccatct gatgactcct ggccgcgagg
    24661 aagcagatct ttcagatgac gatactgcaa cgactaattc agacaataaa tatgcggcac
    24721 aagcaagcaa aatctacgct gcattaggcg gagccgacaa tgtaacttcg atcgataact
    24781 gtactacacg ccttcgtttg caagtcaaag atacaggcct gatcgatcaa aataaaatca
    24841 aagccactgg cgtacctggt atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag
    24901 gtacagaagt acaattcgtt gcagatgaga tggcaaaact acatggtggt gctgcaacac
    24961 gacctgcaca aacaaacacc gtggttaaaa cagaaacatt tgccggtgga acgatcgaac
    25021 aagatattta cgctatcgca aatgggaaac tgattccaat cactgaagtt tcagatgatg
    25081 tattcgcgga aaaaatgatg ggtgacggct atgctgtatt accagaaaat ggagaaatct
    25141 tctctccaat cgctggtacg atcacaaaca ttttcccaac gaaacatgct gtcggaatcc
    25201 aaacagatgc tggtatcgaa gtcttgcttc atatgggcat caacactgtt gacttaaaag
    25261 gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg
    25321 ctttagttga tcttgccgct attcaatctg caggcaaaaa cacggatatg gtcgttgtat
    25381 tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa
    25441 atgataaaat cggctctgtt tccaacaaag cctgagccat cgtcttcccc agactttttt
    25501 atcgataaac aaacaggagc tgagacaaaa atcttgtctc agctcctttt tccgaataaa
    25561 aacggcgttc tcctgccaac tccgcggtaa taagtccagc caaacgtaac atatgaagag
    25621 ctatttttta tttgactctt cttattactt gatgcagaac agctttttta caacctctga
    25681 taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa
    25741 agccattttc acaaaa
//
LOCUS       DASJUF010000031        25653 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJUF010000031 DASJUF010000000
VERSION     DASJUF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.031"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..637
                     /locus_tag="U4B62_001154"
     CDS             83..637
                     /locus_tag="U4B62_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2141327.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(810..1283)
                     /locus_tag="U4B62_001155"
     CDS             complement(810..1283)
                     /locus_tag="U4B62_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2141328.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1295..1831
                     /locus_tag="U4B62_001156"
     CDS             1295..1831
                     /locus_tag="U4B62_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2141329.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1871..2242
                     /locus_tag="U4B62_001157"
     CDS             1871..2242
                     /locus_tag="U4B62_001157"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2141330.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2526..3797
                     /locus_tag="U4B62_001158"
     CDS             2526..3797
                     /locus_tag="U4B62_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2141331.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4042..5067
                     /locus_tag="U4B62_001159"
     CDS             4042..5067
                     /locus_tag="U4B62_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2141332.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5361..6470
                     /locus_tag="U4B62_001160"
     CDS             5361..6470
                     /locus_tag="U4B62_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2141333.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6553..7449
                     /locus_tag="U4B62_001161"
                     /pseudo
     CDS             6553..7449
                     /locus_tag="U4B62_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7465..8289
                     /locus_tag="U4B62_001162"
     CDS             7465..8289
                     /locus_tag="U4B62_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2141334.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8321..9631
                     /locus_tag="U4B62_001163"
     CDS             8321..9631
                     /locus_tag="U4B62_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN2141335.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9779..11038
                     /locus_tag="U4B62_001164"
     CDS             9779..11038
                     /locus_tag="U4B62_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2141336.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11048..11920
                     /locus_tag="U4B62_001165"
     CDS             11048..11920
                     /locus_tag="U4B62_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2141337.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11932..12762
                     /locus_tag="U4B62_001166"
     CDS             11932..12762
                     /locus_tag="U4B62_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2141338.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12802..14985
                     /locus_tag="U4B62_001167"
     CDS             12802..14985
                     /locus_tag="U4B62_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2141339.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15046..16266
                     /locus_tag="U4B62_001168"
     CDS             15046..16266
                     /locus_tag="U4B62_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEN2141340.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16263..17723
                     /gene="gtfA"
                     /locus_tag="U4B62_001169"
     CDS             16263..17723
                     /gene="gtfA"
                     /locus_tag="U4B62_001169"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN2141341.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17800..18906
                     /locus_tag="U4B62_001170"
                     /pseudo
     CDS             17800..18906
                     /locus_tag="U4B62_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19228..19437
                     /locus_tag="U4B62_001171"
     CDS             19228..19437
                     /locus_tag="U4B62_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN2141342.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19430..19579
                     /locus_tag="U4B62_001172"
     CDS             19430..19579
                     /locus_tag="U4B62_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN2141343.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19733..20398
                     /locus_tag="U4B62_001173"
     CDS             19733..20398
                     /locus_tag="U4B62_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2141344.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20682..21767)
                     /gene="xerS"
                     /locus_tag="U4B62_001174"
     CDS             complement(20682..21767)
                     /gene="xerS"
                     /locus_tag="U4B62_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN2141345.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22101..22334
                     /locus_tag="U4B62_001175"
     CDS             22101..22334
                     /locus_tag="U4B62_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN2141346.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22331..22738
                     /locus_tag="U4B62_001176"
     CDS             22331..22738
                     /locus_tag="U4B62_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN2141347.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23875..24393)
                     /locus_tag="U4B62_001177"
     CDS             complement(23875..24393)
                     /locus_tag="U4B62_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN2141348.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25511..>25653
                     /locus_tag="U4B62_001178"
     CDS             25511..>25653
                     /locus_tag="U4B62_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2141349.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQS"
BASE COUNT         8740 a         3923 c         4792 g         8198 t
ORIGIN      
        1 agtttatata tataagttaa tgattatggt aaaatatata cataatcatt aactcagaat
       61 atttttaaga agggaaacaa tagtgagcta taatgtacaa ccgttaagga caaatgaaga
      121 gatacaagat tttcagtttt ggctaagaag aacaagtaat ccggaaagag atagtttttt
      181 atttttgttt ggaatcaata atgggttacg tatgtcagat attataggat taaaagttgg
      241 agatatacga ggcaaaagta agcctattat tgttgaacat aaaacaggta agcgaaaacc
      301 gatttttata gataatttga gagaagaaat tttattatat actgaaggta aagaagaaaa
      361 tgattggtta tttccgagtc ggcaacaagg tcgacatata acaagggatc gtgtctatca
      421 gatatatgca gatatagcag aaaaattagg acgagatgat ataggaacac atacattaag
      481 aaaaactttt gggtatcact actacaagaa aactagagac attgctacct taatgtttat
      541 ttttaatcat agctcgcaag caattacaaa aaggtacatt ggtataactg aagatgaaat
      601 aggagcatca cttcgtggat ttaaactagg tgtataattt aaaaattttt ataaacagta
      661 aagacaaaga ttaaaagcta tttttatcta gaacgatttt atttcataaa gttcgcggaa
      721 taaaagctgg ggttactggt acttggtata ggttcgtgtt atataaacta agtaattaaa
      781 taacattttt aggttggaaa aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa
      841 acgcaaacgc tgcggaataa gaaaaatact gataactcaa cgtttagcag actatctaat
      901 agggatagtc tgcttttttc gtttttgacc atacttttga ccttctgcaa acttccttgc
      961 ttttcctctg tagaactcat gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt
     1021 ttcgcgatat gggtgtatat atcatccata gtggttttaa tatctgtatg tccaagtcga
     1081 acatacactt ctttgacaga cgcaccagat tcaaagagca gactacaatg ggtatgacga
     1141 aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa tgatgatttt caaattgtga
     1201 tttacatagt ctaaataaag gggcttattt tcttctgatg aaaacaaaag ttgttcacca
     1261 tcttttatag gtttgattct catttttaga tattatgccc gcacgtattc atcacatttc
     1321 tattgtgaat cgctttattc gaccgacatt tgacttttat cataatatac tcggtttaaa
     1381 attactaatg aaaacaatta atcaagacga tcataccatg tatcacctgt ttttttcaga
     1441 taatcaccac cgtgtgggaa ctgaactgac tttttttgaa gtacaagaag ggaacaacca
     1501 gttttttgga accaatacga ttgaacgaac gatactaaaa gttccttccg aagcatcatt
     1561 gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt
     1621 ttctggacga cctatcttac gttttgaagg gccagatgca acacgattag ctttagttcc
     1681 cttacgcgaa tttgaagata ttgatgatta tttcccgtat gtcacagacc agatccctac
     1741 agagcatgcc attttaggaa ttgatgccat tcaactacgc gtacaatatt ccgatgcgac
     1801 tgaacgagag ttattgtcgg taggctggta gcacaaagaa tacgtcagtt tcttttcggg
     1861 tcatcaacga gtgacaatct tagaaaatca agacactcag ttctatcaag aagttcagat
     1921 tattcaagat cgtgagaatc cagttgccat tgaagggatt ggtagcgtcc atcatgttgc
     1981 gtttggcgta gaaaataaat ctgatttaca acgcatcgac aaacaattac aagagcgcaa
     2041 ttttataaat tcaggaataa aagatcgtga attttttgta tcactttatt atcgtgaccc
     2101 aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga
     2161 aaatcaaagt ccacgctttg aagaaattcc tttattttta ccgcagtact taaatttcat
     2221 tcgtgaacaa gtggagcaat aattagctaa tcaaatccat gacgaagagt aagaggttga
     2281 ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat tctaaagaat gttgatttga
     2341 tgcggttcct atttttataa actatcattt tcgactaata aaactactta tgagtcagcc
     2401 tctctttttt tcttgatttt tatacctctt tctaggtaaa ctaaacatct gccaaaaata
     2461 ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa ggggagtacc aaatgaacga
     2521 gtatattgaa attatccacg caagagaaac aacttaaaaa tgtcagtctc aaaattccca
     2581 aaaataaaat tagtattttt accagcgtat ctggttcagg aaaatcatcg attgtttttg
     2641 atactattac ccaagaagct ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt
     2701 tatttttacc taaatacagt cgtccagagg ttgatgagat tgataatctt tctacggcga
     2761 ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc aacgttaggg acgattacta
     2821 acatcaatgc gttatttcgt gtgttgtttt catgttttgc aacacctagt ttaggatatg
     2881 caaatgctta ttcatttaat gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta
     2941 agacgattgc atttttaata ggattgcttt atttttcggc tattgataat gctgattttg
     3001 tattttctga tccatttacc gcagaagttt tgtctggaat tgaggaacag gcaaagaatc
     3061 atggttattt tgtattggtg cataatgtta attcagcaga agatgttaaa actattcaga
     3121 gaaattggcg ttttgagggg ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat
     3181 taaataacgt ttttgatggt cctgttgttt atattgatac gcatctttca aaagagacat
     3241 taagagaact tgaacaacaa atgaatcgtt tatttgtaaa tacaaatgat taccaagcta
     3301 gtacattggc agtggaacat ttgattcatt atggtcatga gaaaatagcg tttctttctt
     3361 ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg acatcaagct tatatcgata
     3421 cattaaagaa atatcattta acctttaaat cgcattggat atataacaac tctgaatttg
     3481 agcgaattta tcaagaatta gctgaattta ctgcaattgt tgttactgga gattatttag
     3541 cggctaaatt agtaaaattt ttaaaaaata agaataaacg agtgatggaa caattatcga
     3601 ttattggatt tgatgatatt acttttgcag aattaatgga tcctgcgttg acaacaatac
     3661 gactaaatcc aggtaacaag ggggcgattg cttttcacca actgattgga ctggccaaac
     3721 aagaagaaat aaaaaatcaa gtactactat tagagggaga attgatttct cgagactctg
     3781 ttcacaaaat caaataaaaa agctctgtga aataagtata gttaacaata aaatatccga
     3841 acgatagaga gaatgaactg tgatctagca gttcctcgtc gcaaattcac tagaattagc
     3901 attatttact ctaaattgtt cggatatttt ttcttgtttt taattatgat tacagtgcct
     3961 atttttaacg aaatttgctt attttcaaaa tgtatgatta tatgttagta aacactttca
     4021 aatatgaaaa gaggagctat tatggtagga attagggatg ttgcaaaacg ggcgggtgtg
     4081 tccattgcaa cagtatcgcg cgtgctaaat gaggatccga cactttcagt aaccgatgaa
     4141 accaaacgaa agattgcaga atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt
     4201 gcaaaaaatt taacaaatat tgcattaata acgacagttt ctgaagtaga tgagttggaa
     4261 gatccctatt ttcgaacgat tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc
     4321 aatttgaaga aaattattcg tttgtcagaa acaccgttgg agcaagacga tttgctaaaa
     4381 tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg tgattgaaga agtatcagca
     4441 attaattcga atgtcgtagt gattgatgac cctagtattg aagctgaagt agatgcagtt
     4501 tatactgatt taacaaaagc aacgaatcag catttagatc gattgtataa aaaaggacac
     4561 cgaaacattg cgtttatcgg aggaggacgt gttcgtttag atagccaagg aaataaacac
     4621 gtgtcagatg atgattatcg aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta
     4681 gaaaaatata gtcaaattta tttagaaggc tggaccacac ttgacggaat gcaggcagca
     4741 gatttacttt tgaaaaaaca tgctaaagat aagttgccga ctgctatcat ggtaggaaat
     4801 gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa atggtctaca tattccagaa
     4861 gatatttcga ttgtgagttt tgataatatt gaagttgctg aatttttaac acctgcatta
     4921 aatacggtga atgtgaatac agaagaaatt ggaaaactag cagttcgcat ggttaatgaa
     4981 cgaataaaag aagttagaaa tgtttcgatc aaagtgattg tttctaataa tattattgtt
     5041 agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt
     5101 tttgtttact aatattttta gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt
     5161 gttgttaaca aaaagaaaac gcattcaaaa caggaggaat taagatgaaa catttcaaaa
     5221 aaatagcttt aacagggata gctgttttaa ctgggacatt actcgcagca tgtggtgggg
     5281 gagatagtaa ttctaaagac gggcaagtaa ctttaagcta tgcgagttga gatactgacc
     5341 aagctccagg actaagaaaa atgttagatg aatttgaaaa acaaaatcct gatatcaaag
     5401 tagaaatgga gacaacgcct tgggatcaat attgggttaa attagaagct gcaacaacgg
     5461 gaggtaatat gccagatgta gtaaccatgc actctagcga atcctataaa tatatgtcac
     5521 agaatgcttt gatgggactt gatgatgtta tcaaagaaaa tgatattgac atgggaaact
     5581 ttactgaagg aattgctgat ttctatacgc ttgaagacga actatacgca atccctaaag
     5641 atgcttctgt agtggggcta tggtacaaca aagaattatt tgataatgca ggtgtcgatt
     5701 atccagatga aacatggaca tgggaaacac tcagacaagc tgcaattgat ttaacagatg
     5761 aatcaaaagg catctatggt tttgcatcac atattggaac agagactggc tactggccat
     5821 ttatttatca aaatggggga gaagtatttg ccgatgacaa tacaaaatca ggtttgaata
     5881 ccccagaagc acaagaagct ttagcttttt atcgagatat tgcacttgtt gatcatgctt
     5941 caccaacaat tacagaatta caagatcctg ataagttaag tcgttttcaa tcaggtagat
     6001 tagcaatgat gattgaagga aactggtttg caccgggatt tttgaaaaat gactatctag
     6061 ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt taatgcaaca attacaaatg
     6121 gacttggttg ggctgccagt gcgagtacaa aatacccaga agaagtgaaa aaactaatgg
     6181 catttttagc tagtgaggac gccaataaaa tccaagcgga aacgggggca tcaatcccag
     6241 cactaaaagg ctttgaagac gagtgggctg cttcttcaga tacaattgat ttgagtgctt
     6301 ttggaaaaat gttggagtat ggagtccctc gtccatacaa taaagatgga ttacgagctg
     6361 aacaagcaga agttcaaatt attaaccgta ttctgtctgg tgaagtaact gttgaagatg
     6421 gaaccgctca aattgaaaaa gaagtcaatg cgatattgag cggaaattaa attagttaat
     6481 tatacaatat acaggcgaaa ggattctatt tcgtctgtat attaatggat gaataccaaa
     6541 aagggggata ttatgaaaaa tcgaattttt ggaaaatatc gtaaagatca agtgatatgg
     6601 ggctatattt ttatttttcc aacggcatta ggattaattt tattaaatat atggccagca
     6661 attcaaacag caattttaag ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc
     6721 ggattaagaa attaccaaaa aatgtttgca gacggtgaag tttttcagtc tttattaaac
     6781 acattaattt atgcagtggt ttcgatacct agtattgttg tattatcctt gttggtggca
     6841 gttttaatga atagaaaagt taaaggattg tcgatttatc ggacaattta ttttctacca
     6901 gtcgttgctg caccatccgc agtcgcaatg atttggcgct ggatgttcaa taatgattat
     6961 ggactgatta ataacatgtt ggctaaaata ggtctcgatg gaccagcatg gttaacggat
     7021 ccatcgattg cgatttattc tattatcatt gtaggagttt ggagtgctat tggttataac
     7081 atggtgttat tactagccgg cttataagaa attcctaaag attattatga agcagcggaa
     7141 attgatggtg ctagtccaat ccagcagttt ttcaacatta cgttaccatt agttacacca
     7201 acattatttt ttgtagttgt aacaacagtt atcaatgctt tccaagtatt tgatgttatt
     7261 tttatgatga ttactcctaa tagtaccgcc atgtttaaat cacaatcatt agcctattta
     7321 ttctataaac attcatttat tttaaatgac aagggatatg gttcggcaat cgttatgttc
     7381 cttttagtga ttatattaat tattactgca attcaaatga agttgcagaa aaagtgggta
     7441 aactattaag gaggctatca aattatgaaa aataaatttt tttcaaatgc gccaatccat
     7501 atcatgctta ttttaggtgg tattgcaatg gcattacctt ttatttggat gattttaact
     7561 tcagggaaaa cgttggttga atctacccaa attccaccta caattttccc tgagaaattt
     7621 cagctaagta actatcaaca agtacttgat ttattaccat tcatggcttt ttatatcaat
     7681 acatttttaa tggttgttgg acgagtgatt ggctcggttt tattttcagc aatggctgca
     7741 tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc ttttcagctt agtacttatt
     7801 caaatgatga ttcccggttc catttttatt actccacagt atttattagc acaaaaattg
     7861 ggtattttaa atacaattcc agcattaata ttaccagggg tagttagtgc gtttggtacg
     7921 tttttattaa gacaattttt cttaggttta cctgatgaat tggaggaagc ggcctattta
     7981 gaaggtgcca atatttggac gatctttttc aaaattatgt tgccgcttgc taaatcagga
     8041 ttggttgcac ttggaatatt cacagcgttg tttgcattta aagatttaat gtggccattg
     8101 attgtcaata cttcaattga taagatgcct ttagctccgg gattagcaaa cttacaagga
     8161 caatatgcca cgaacttccc acaattaatg gcaggttcgt tattagcaat ttggccaata
     8221 gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag gaattgctac aacaggtagt
     8281 aaattataaa actattaatt agtgattgga gagtaagtac atgacaaaaa ttacatttat
     8341 tggtgcagga agtactattt ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag
     8401 tctacaagat gcacatattg cattgtacga tattgatgaa aaacgactaa aagaatcaaa
     8461 tttaatgctt aaaaatatta ataaaaattc gaatgaaaat cgtgctgata ttgtttctta
     8521 ttatgatcgc aaagaagcgt taagagaggc tgattttgtt atcaatgcta ttcaagtggg
     8581 tggttataaa ccttcaacag tgattgattt tgatattcct aaaaaatatg ggttgcgaca
     8641 aactattggt gacaccatgg ggattggtgg gatttttaga accctacgga cactaccagt
     8701 aatgttagac tttgctaaag aaatggaaga agtttgtcca aatgcttggt tattgaacta
     8761 tacaaatccg atggcgatgt taaccatggg tgttttaaaa gcgacgagta ttaagactgt
     8821 tggtttatgt catagcgttc aagtatgcgt accagaattg tttgagcatt taggaatagc
     8881 cgatcaatat gacttaaatg actttcaatg gaaaattgca ggcattaatc atatggcatt
     8941 cttgttggaa attacaaaaa atggggaaga tttctatcca acaattcgtc agttagctgc
     9001 agaaaaacca aatccacatc cagattctgt acgttttgaa ttgttgaaac gttttggtta
     9061 ctatattaca gaatcaagtg aacacaatgc agaataccat ccatacttta ttaaatcaaa
     9121 atacccagaa ttgattgacg aattagggat tccgttggat gaatacttac gtcgttgtga
     9181 acaacaaatt aatgattggg aaagcatgcg agaggacatt gtaaataatg gggagttaac
     9241 acatacgaga agccgtgaat atgcttcata tatcatggat tctattacga caggaactcc
     9301 gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc acaaacttac cgacagatgt
     9361 ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg caaccaacct atgtcggtga
     9421 attgccaaca caattagctg ctttaaatcg gacgaatatc aatgttcaag aactgacagt
     9481 agaagccgct ttaacactag ataaatcaaa aatttatcaa gcagcgtatt tagatccaca
     9541 tctatcatct gaattgtctt taacagatat caaaaattta gtggatgatt taatcgaagc
     9601 acatggggac tatttacctg aatataatta aaaagaagct gaaagagtaa ggaattttca
     9661 attccttact cttttcttag ttaaatttag taaaaatatt gacaaaacac ttagtaaaca
     9721 gttacattag tgttaacaaa aagaaaacgc attcaaattg tataaggagg agcataaaat
     9781 gaagtggtct aaaaaaatta ttttagcttg tatagcacta ggagcaacga tctctttgac
     9841 tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta gaaatagagt tttttaatca
     9901 aaaaaaagaa atgactcaaa cgattcagga gattgctaaa gattttgaag caaaaaaccc
     9961 tgatattcat gtaaaagttg tggatgtacc gaatgctggt gaagtaatta aaacacgtat
    10021 gcttgcaggc gatgtaccag atgtcattaa tctttatcct caaagtatag aattgaaaga
    10081 atgggcgaaa gctggttatt tagaggactt aaccgaagaa ccgtatttag agaatatcaa
    10141 aaatggatat gcacaacgtt ttgctattga ggatcgtgta tatagcatac cattaaccgc
    10201 taatgtctat ggtttttact ataacaaaac tgcttttgag gaaatgggga ttcaagcacc
    10261 agaaacttgg gcagaatttg aaaaaatagt agctgagatt aaagatcaaa acaaagtacc
    10321 gtttgcgatt gcaggagcag aaggctggac tttaaatggt tatcaccaac tagctatagc
    10381 gactgctgct ggtggtgaag aggaagcgaa caatgtttgg cgtttctcag aagttaatgg
    10441 aattaatgct gaaagcaaag aaatgcaaaa agattttaat cgattagatt tgttaagaga
    10501 accaaaagct ttgcaaaaca actggcaagg tgccggttat aacgatacag tagttacttt
    10561 tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg gcaatgccga tgattcattc
    10621 acaaaatcca gattttgaag taggaacttt tccttttcca gcagatgaag caggtcaaag
    10681 tctaacgatt ggcgcaggag acctagcctt atcagtttct gcaacttctc aacataaaga
    10741 agaagccaaa cgttttgtag aatatatgac aacacctgaa gcgatgcaga aatattatga
    10801 tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag aatactcagg attcaccatt
    10861 aagtggttta actgaacttg cttttactga tcgtcattta gtttggttag ccaaagattg
    10921 gaatagtgaa aatgattttt ataccttaac aacaaattat ttacacaatg gcaatcaaaa
    10981 agccatgatt aacgcattaa atgctttctt taatccaatg aaagctgacg gaaggtagga
    11041 aaataaaatg aaaataaaag aaattttaaa tcgttactgg gcaatcattt ttgtagcaat
    11101 tccaattatt ttgcagatga tttttttcta ctttccaatg gttcaaggtg ctttttacag
    11161 tttaactaat tggactggtc tgacttataa ttttgatttt gtgggtttga acaattataa
    11221 gattcttttt tctgatccga agtttatcaa atcgattggc ttcactttaa ttgtgacgct
    11281 ttgcatgatt gctggacaaa ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa
    11341 tattaaaggg aaaacattct ttagagcatg gttctttttt ccagcggtct tatcgggcgt
    11401 ggttgtttct ttagtattta aacaaatttt taactatggt ttaccaaaaa ttggtagtat
    11461 gttaggcatt gaatttttac aaaccagtct tttaggaaca acatggggag cgatttttgc
    11521 agtaattttt gttttacttt ggcaaggaat tgccatgcca gtcatcattt tcttagcggg
    11581 ttttcaaagt attccacaag aaattattga agctgctaga atggatgggg cgaatgataa
    11641 gcaaattttt tggaatattg aaatcaccta cttattacca agtatttcaa tggtatttat
    11701 tatggcatta aaaggtggct taacagcatt tgatcaaatt tttgctttaa ctggtggtgg
    11761 accaagcgat gccacaacat ctttggggtt acttgtttat aattatgctt ttaaaaacaa
    11821 tcagtttggt tatgcgaatg ctattgcttt agtcttattt atcttaattg ggattgtttc
    11881 actggcacaa ttaaaattat cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca
    11941 tcaaaagaaa aaaatatttc taaatacttg cttttattat tagggggctt catgatttta
    12001 gttcccttag tttcaacgct attcggttca tttaaaacaa cacccgatat tatgaataat
    12061 ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc gaagattgtt agcggatgga
    12121 attggtcatt acttttggaa ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca
    12181 gcaattattc ctatggctgc ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac
    12241 ggattgatgt atttattttt aattttagga atttttgttc ctttccaagt aattatgatt
    12301 ccgattaccg ttatgatgag taaaattggt ttagcaaata tgtggggatt aatcttactc
    12361 tatctcactt atgccgtacc acagacacta tttttatatg ttggttttat taaactaaat
    12421 gtgcctatca gtctggatga ggctgctgaa attgatgggg ctagtcactt tacaacttat
    12481 ttcaaaattg ttttcccaat gttaaaacca atgcatgcga cgactattat tcttaatgct
    12541 ctatggtttt ggaatgattt catgctacca ttattaattt taaatcgtga ttctaatatg
    12601 tggacactac cattgtttca atataattat acgggacaat attttaacga ctatggtcca
    12661 agttttgctt cctatacagt aggaattgtc acgattacac tagtttatct tgctttccaa
    12721 agaagtatta tagctggaat gagcaatgga gcagtgaaat aaccaataaa ttattctcat
    12781 tataaaggag ttcataaact tatgattata tttgacaatc acacacaaac atttcattta
    12841 caaaataaaa aaattagcta tctattagga atcgaagaaa ataattattt aacgcatctt
    12901 tattttggaa aaaagattac gcactattct ggtgggtatc gctacccccg aacagatcgt
    12961 tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat tatctttaga cacattacca
    13021 ttagaatttc caggatatgg acatggtgat tttagagaac cggcgcataa tattaaatta
    13081 ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct acgaaattca aaaaggaaag
    13141 cccagcttag cgggattgcc agccttatat acggaagatg atgatgaagc tgaaacgctg
    13201 attattcaat tgaaagacgc gattagtgga ttgactttac agttgttcta cacaatttat
    13261 caaaatcaca gtgtaattat tcgttctacg caattagtaa ataatagtga tgaaacagtg
    13321 gagattaacc gcttagctag tcaatcaatt gattttccaa atcgtccact cgagttaatt
    13381 catttaaatg gtacctgggc aagagaacgt caaatgacta gagaaaaaat tcatacaggg
    13441 ataaaagtat tagatagtaa acgcggatca agcagccatc aacaaaatcc gtttgttgct
    13501 ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat atggatttaa tttagtttat
    13561 agcggtaatc acgaaacggt gattcaaaaa gatccatttg accaaacacg ggtcattatg
    13621 ggaattaatt catttaattt tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca
    13681 gaagtcgtga tggtttatag taatgaaggt ctgaatacgt tatcacagac gtatcatgaa
    13741 ttgtataatc accgtttaat tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc
    13801 aacaactggg aagcaacgta ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat
    13861 gatgcgaaga acttgggcat tgaaatgttt gttttagatg atggttggtt tggcggtcgt
    13921 gaaaatgact tcacttcatt aggtgattgg gttgaaacag aaggcaaact tgcttgtggt
    13981 ttggaaaatt tagccaagca agtgcatgat aacgggatga aatttgggat ttggtttgaa
    14041 ccggaaatga tttctgaaga tagtgaacta tttcgagcac atccagattg ggcattgggt
    14101 attccaggtc gtgaccgctc aattagtcga aatcaatatg ttcttgattt ttcaagacaa
    14161 gatgttcgtg ataatatcta tcaacaaatg acggcgattt ttgatcgtgt accgattgat
    14221 tatgtgaagt gggatatgaa tcgaaatatg acagaagtct attctgtctt gttggactca
    14281 acgatgcaag gtgaagtggc tcatcgttat atgttagggt tatatgaatt tttagaaaag
    14341 ttaacaacga attatccaac tattttattt gaaagttgtt cgggtggagg cggtcgtttt
    14401 gatgcaggtt tcttgtatta catgccacaa acttggacga gcgacaatac cgatgccgtg
    14461 gctcgtctga aaattcaata tggaaccagc ttagtgtatc caatttcaag tatgggtgcg
    14521 catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc
    14581 aatgctgcga tggctggtgt ttttggttat gagctagatc taacgcaatt aacttctgaa
    14641 gaaaaagcag aaattaaaga acaagttgcc ttttataaaa agcatcgcca acttttgcaa
    14701 tatggaactt tttatcgctt ggaaagtccg tttgaaggca atgatactgc atggatgttt
    14761 gtctcaaaag accaaacaga agccatggtc ttttatttcc gagtattagc agaagctgcg
    14821 catccattag tcacattgaa attagcagga ttagcagaag aagcaactta tcaaatggaa
    14881 gatgtaacat ttgctggcga tgaattgatg aatctcggtt tttatattga ctcagaacta
    14941 catggtgatt atgcgacaca acgcttttat ttgaaacaga tatagtagct tgaatttaca
    15001 atataagtgc aacaccaaaa gagactcatt ccaaacgaag aaaatatgtt ttatttacac
    15061 gcatttacta aaaagcagcc tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc
    15121 tatgcaatga ttaactattg gttagataaa ggactaggcg gatttagaat cgatgcgata
    15181 ttaaacatca aaaaaaaact agagtatggc atctttgaat ctgatggaga agatgggtta
    15241 gccttcattg ggccctggat tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg
    15301 agagagcgga catttaaacc acataatagt atgacggtag cagaagccga tgttcctaat
    15361 gaacgtttag atgaatatat tggtgaagat ggctattatt cgatggtttt tgattttagt
    15421 tatacggata ttgatgtacc tgaaactggt gaatggttta aagatagcca atggacatgg
    15481 acggatatgc gtaccaatat ttttacgaat caattagtta cgcaagataa aggttggggc
    15541 gccttatatt tagaaaacca cgatcaacca cgttcaatta ataaatatat tccagaagaa
    15601 tggatcaatg attatagtaa aaaaatgttg gcgacgcttt ttatgttact aagaggtacc
    15661 ccttttattt atcaaggaca agaactagga atgaccaaca tagaaatggc atcattagaa
    15721 gattttgatg atgtagctac gcattctcag tataaacggg cattagaata tggattggca
    15781 ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg ataattctcg cacacctatg
    15841 caatggaata cgcaaaaaaa tgccagattc tcaacttctg ataatgtatg gttaaaagta
    15901 aatcctaatt atccagaact caatgcagaa gctcagttaa ccaatcaaca gtcggtattc
    15961 aattattatc atcaattaat taaattaaga aaaaatagtg tatacaaaga cgtgctaatc
    16021 tacggacaat ttctaccagt gaaagataaa aatgaacata ttttgcttta tgaacgccaa
    16081 ttagaagata aaatcgtatt agttatctta aacgttacag caacagaaca agaatattat
    16141 gtagatgaac agtaccaaca agtattaatc aataattatg aagatgtttt attagatcaa
    16201 agtcgaaaat tgcgtttacg accgtttgaa agtattgtat tagctaatta tggaggacaa
    16261 ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc agatagttta ggaaaaaaca
    16321 tgggcgaatt aaaacaagtc ttagacaaac atttacaagg tgttattggt ggtgtacatc
    16381 tgttgccttt ttttccatcg acaggtgatc gaggctttgc gccgagtgat tatacgacag
    16441 ttgatccagc tcttggaaca tgggaagatg tggaagcatt aggtgaagat tattatttga
    16501 tgtttgattt tatgattaat cacatttctc gtgaatcaaa atttttccaa gatttcaaaa
    16561 aaaaccatga gcagtcacca tacaaagaaa tgtttatccg tattcatgaa tttttcccag
    16621 aaaatcgacc aacacaagaa gatattgatt taatctacaa gcgtaaagac aaagcgccgt
    16681 ttcaaacggt agaatttgcg gatggtacga cagaagaggt ttggaatacc tttggggaag
    16741 aacaaattga tttagatgtg accaaagaag ttgtgaaaga atttattcga gaaaccatca
    16801 aagatatggc gaaccatggc tgttctttaa ttcgattaga tgcgtttgct tatgcgatta
    16861 aaaaattaga taccaatgat ttcttcgtgg aaccagatat ttgggaactt ttagacgaag
    16921 tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc agaaatccat gagcattatt
    16981 ccattcaaat gaaaattgcc aatcatgatt attatatcta tgacttcgcc ttgccaatgg
    17041 tcactctgta ttcactttat agtggaaaat cagaacgttt agccaattgg ttgaaaatga
    17101 gtccgatgaa gcagtttaca acattggata cgcatgatgg aattggtgtt gtggacgctc
    17161 gtgatttatt aacagatgaa gaattggatt acacctctga ggaattgtat aaagtcgggg
    17221 cgaatgtgaa gaaggtctat tcaagtgcca attacaataa tttagatatt tatcaaatta
    17281 acagcaccta ttatagtgca ttaggcgata atgaccgtag ctatctgtta gcacgtgcga
    17341 ttcaagtatt tgcgccaggg attccacaaa tttattacgt tgggttatta gcaggaaaaa
    17401 atgatattga actgttggaa gcaacgaaag aagggcgtaa tattaatcgt cattactatg
    17461 atttagccga aattgaagaa gaagtgcaac ggccagtgat tcaacaatta tttgcacttt
    17521 taaaatttag aaatgaatct gcggcgtttg atttggatgg aacgattgaa gtagcaacgc
    17581 catcagaatc agaaatagtg attacacgta aaaataaaaa cggtagtgat agtgcaacat
    17641 taacggctaa cttggatacg aaagaattta tcattgagtc taaagatata aaaattttca
    17701 ctagtgttgc attaaaattt taacctattc catagaacaa aaaatccttt atactagcac
    17761 ttggatgact tatccaagac caataaaaag gactcataca tggataagta taaaacaaat
    17821 acagcattta ataaatggtt ttctgccata aaattaggaa aactttctgt caactcccaa
    17881 aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag
    17941 ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt
    18001 atcgaccgat cgctccaact agaaaccgat gtgtcatcga ttagtcatac ccaattgtct
    18061 cgagcattta atgcacttga acctaaagtg ttagaagaaa tttttcaaca actgttagaa
    18121 aaagttcaac atcaaatcaa accaaccaag cgaaatcatc tgtatctgat tgattccagt
    18181 acgttttcgc ttagtttaaa acgtcataag tgggcgactt tccgtcaaac aaaatcaggt
    18241 gtcaagttac acttaaacta ttgttatatg gacgatacta cgatgtatcc aactgatttt
    18301 accttaacaa atgcccatga acatgatctc aatcaactac cagtattggt gaatcaacca
    18361 gaggcaactt acgtgtttga tcgtgggtat taggactttg aaaagatgga tcagatgcat
    18421 tgggatggct atttcttcgt cactcggatt aaaaaaaaca ccaaagttca cgtgatagat
    18481 actctggaaa catcgccaga aacagaaatt ctacgagacg agctggttcg tttaggatct
    18541 aaaacctatc taaccgccaa tttccgacta gtcactgttc aagataagaa cggacgtgtg
    18601 tttcaattta tcaccaatcg aatggacgtc tcttcaaaag aaatctctga tatgtatcac
    18661 gctcgttggc aaatcgaatt gtttttcaag cacatcaaac aacacatgac cattaaaacc
    18721 ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga ttttaacgat gatttccgct
    18781 ctcctcacgt ttctcatcaa attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa
    18841 cgattctttc gttatttact gtttcagccc tttgaatgtt gtctagaaaa attaattccc
    18901 acatgagaaa aaagaaattt ggctcgttac ttttggtttt cagttgaata acagataaaa
    18961 aattttctgg aaaaagtcat cctctgcgtc agcacagttt tttttgactt attttcagag
    19021 gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat
    19081 tagatttcta atgtgcattt tttgagtatt aaggagcaag tatacaaaga tttataaagc
    19141 tgattaaatt tgaataaaaa tatgcaggaa cgctactatt tttagtagtg ttctttttta
    19201 tatttgaata aaaaatgtgg tggttaaatg gtggttataa caaaaaatga attaatgaaa
    19261 ttaggttatg ggaagtatca gtctgagaga atcatacgac aggcaaaagc aattatggtt
    19321 tcaaaaagct atacctatta cgaaaataga agattaggac tagtacctgt tgaatctgta
    19381 gaagaagttt taggtatgaa gatagaggta gaggaggatt ttcagaatca tggttaattt
    19441 taaaaaagat aaaaatggga aatatcattt tactgctagt ttgggattcg acaaacgaac
    19501 agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt
    19561 ataccgaact tgtttctaat tatggaaaag aagcagaaac ttctcaaagt actatgttgt
    19621 ttgaggagtt tttttataaa ttattcctac cttattatca agggaaagta gaagaaagaa
    19681 catataaaaa tcggttatct acaatcaaaa aaacttttct tatttcttta aaatggagtt
    19741 aagagaaata cgtccaattg acattcaaaa gtggcaaaat aatttattga gcaaatataa
    19801 aaattcttat gtaagaaaca tgtatgggat ttttcagatg tcattagatc gagcagttgt
    19861 tttgggacta cttgaaaaaa atcctgcaaa agttattgga aatgtgaaga aaaggaaaac
    19921 tacagttgat ttttggacaa aagaagaatt tacaaaagtt atttcaactt tttatattga
    19981 tgatttgtac caacattttg gttttatttg tatgtggtta ctttttatga ccgttatgcg
    20041 tttgggagaa gccactgcat taacttggaa agatattgat tttgaaaata aaaacttgcg
    20101 tgtgaataaa acactctatt ataagagagc caaaacctat gagtttgtag aacccgaaac
    20161 ggcttctagt attcgtacaa tcgaactaga cgacaataca attacctttt taaaagcttg
    20221 gaaggagcgt caagtttcaa taggaggcat gtcgtttgta ctttcttata atggaacacc
    20281 tactcagaaa tatacaatcc gtcatattat gagaagacat gcaacgatcg ttggtgttca
    20341 tataatccgc atacacggat taaggcatag tcatgcttct ttgcttatat cacaataaaa
    20401 agatgcactt cgaattaaag aaaggttaaa acatgctgat gtacaaacga ctttaggaac
    20461 ttatggtcat ttgtatctaa attcattgag agagataaca aatgatccca cagattatat
    20521 ccaaactaat ttttctaaaa tcaaaccgac acctatttac gataataaaa aataaatgat
    20581 aaacaaaagt aggatcacac caaaagaaac acactagaaa aactataata caggcttttc
    20641 tagcgtgttg tttcttattt ccgcctattt atacattata ctcataaagc atttaatgca
    20701 tcgatcgtag ccgcattgtc tatatgggta tataaagttg ttgtattggt acttgaatgt
    20761 cccaattgtt gggcagttaa taccaatgaa tttgtctgtt ggtagagtct agtagctaat
    20821 gtatgacgca gtttgtgagg gctgacttga attctaaagc cttttgaata ttttgctacc
    20881 atccgttcga ttgcaatacc ggatatacgc ttggcttctc cttggtaaat tgctagaaat
    20941 aaagcttttt cgttttctgt tactttatat cgttctttcc gaatagctag ataattagct
    21001 aaatattcca ttgcgaatgc tgctatattg acagcatctc ttttatttcc ttttctagtt
    21061 actaccacat tattcttatc taaagatagg tcatctaaat taatatttat aacctcggag
    21121 agtctcaatc cagatcctaa aatcagtgca ttgatcgcca ggtctctttc tacatctcgt
    21181 tgataataga tcaaagctcg tttactcaaa gtatttttat attcattggc aatataatct
    21241 agataatttt gtgtatcctt atctagaaat aattttttct caatggcatt ggcacgtgca
    21301 gctaacgtag ttttatctgt gatggttgct acttgatcca taggattatt gtctaagtat
    21361 ggttttccta gaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgcc
    21421 gtgatatttc tattgatagt agaagtagat agacctaagt tattattatc tttgagctta
    21481 gtttttgttg tctctttttt tacattttct tttgtgcgac gtttcaaatg agatttgtac
    21541 aagtttaggt cataggcagt taactgtctt atcgaggtga tagaagtatc aggaacgata
    21601 gactcactca gaagccattc ataaaagagc ttataatttt ttagatattc taaagtggtt
    21661 cgaggtgatt gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata
    21721 tgaagtatga ttgtatctat tttttgatat atttctgtat ttttcatgtc attcatcctt
    21781 tcaataagta ggggagaaga gtatctctat aattggacta taccaattat agaatgtttt
    21841 gtcaattggt atgctctaaa atttattaat ttgtttcaaa aaaagcatat aaacaggtat
    21901 acgtttaata atttaaaata aatggtatac ataaataggg aaggaaatag aagggagcaa
    21961 tctaaatttt ttgaattttc ttaattaatt atatagaaag aacaatcttt ctatagagtt
    22021 agacatgaga gggactatgc ttgatttaaa atcaacaagc gtatatactg tatatacaaa
    22081 gaaaaaggag ggagaagact atggcaatta aagaaagaaa atttagggaa gtggggaact
    22141 cagtagtatt aactatttca aaggagtttc ttaaagaaaa tgggttaaat cccggagata
    22201 cggttttttt agatgaagaa aaacttcgag cggcaattac aaaaaaacaa gccttatctg
    22261 atgaaattga tttagcaata tctcaagcat taactgaata tgatactact tttagagagc
    22321 ttgtcgataa atgagtattc attacctaga agccaaagat attataaaaa tgaatgtaat
    22381 ccaaataaaa aaatactctc ctaatgaacc ccttggtgta aaggatgcga atgctttaga
    22441 aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa gaggtttatc cttcgattta
    22501 tgaaaaagca gctatcctac ttatacaatt gataaagaaa catccttttc acaatgcaaa
    22561 taaacgtact gctttcttag ctacttttgt ctttttgaaa atcaatggac agcttttagc
    22621 tattgatcaa aaagatgctg taaatttagc ggtatatatt gctacgtatg ataaggattt
    22681 cgatcaacta aaatatgaag tgatcaacac tataaagaca catacgcagc ccatttaagg
    22741 agttactgta tgtgtcttta tagggttatt agctgctata gcgaaataat tgaagtaaat
    22801 tacaaatagc tataattcaa atccaactat atagaaaacg taaattttct atatagttgg
    22861 attaagataa cgactattta ttttatcata tttttactat accctatact tttacttggt
    22921 atatttatga ataatcgcaa atttacccct atactactag tttgtaagtt agattaataa
    22981 aaatactaaa gttatttgtt cttttattaa atacacctac aacttataaa ttgatggtat
    23041 ttagataaca cgaaagaata aaaataacag ctgatagtaa agtttgttga cgattgctac
    23101 tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga aaacttatga aatgaaagac
    23161 cttttacatc ttgcagaaat cgttactggg attaaactaa gcaataaaaa ctaatgttcc
    23221 cctcattctt tgaacttcta ctatttgatt tatttattct gataaatgac gatttcacct
    23281 tcaggatctt caaatcccgt atagaattta tttaagatct cttcagtatc agtaatagga
    23341 taggcgatag gccacatacc ctttgatggt tagaataaac tgctcaaaat ctgtatcgta
    23401 tgttggcttt agcatattaa caaaggctat ctccaaatta taaaccataa gcgttgaatc
    23461 gttcttaaca atttcaattc ttcctaaccc aaagtcagaa gatataatta ccggacttgc
    23521 cttacccttc atgtgggttg ttgagattcc atatttaaag ggaattgtcg gtactatcgg
    23581 cggtctgaaa aacatcccaa aaataaaaaa atctttcccc tgctagtctc ctaagtccct
    23641 ttgtagcgga tatggcacca taatctcatt ttatcgaagt gagtcatggt ttatctgttg
    23701 gccaaccaaa attgatacaa tctaacgatg caccctctac ggcattctag gggagtgcac
    23761 ttgcattcac ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg tatgttctta
    23821 ctgttaacat acaagaggtc tcctttccac aattgcttag cagtaaacgg gaatttacaa
    23881 agctaatctc ttccattttc ttatctttgt tctaaaagtc ccaaatggag caactgtatt
    23941 tacatgaata aatttatata cttcccatac agccgttttg gttgcctcgt ctgcccattt
    24001 tcttttgtgt gcagtgaata gatcttcatc actcattgaa tcaatcatca tgttaatatc
    24061 atcaatatta cgattgagaa tttcctctaa ttctttcaat ggtagatgtg cataggtatt
    24121 tgtaaaccac ttatataact ctcctaactg attccattta aattgatcag aaggcgtttt
    24181 aacagataat ccatttcttt catcattttc ccattttaga attaaattcg tccaaccaac
    24241 ttgatatgct aaattttcag caggtgttct atccacttcc tcaactcttt tatccttcaa
    24301 ttcttcagga atagtttcaa attctaaaat atatttagta taagtcctat ttatttcttt
    24361 ttttaattct tctttatttt tatatgttct cattatatta cctccataaa ttcctaattg
    24421 tctcctttaa ttcatctata atttcatcca cgatatttta atccgttcta caatgcatat
    24481 ctgatatgca ttgactgtct aactcatatg tgaatgattt ataaaatatc ccaacgtctc
    24541 atctaaagaa atgttgatcg tcagcaagga gctaaaaaat acgaatatat ttcttttgta
    24601 gtaaaatcgg actggtttgc atatcatttt cttcgtaaat atcaaaatca tgttaactac
    24661 tatcaattac taataaaaac taccacttta agcgttaaga actgttaagt ttagttaagt
    24721 gaggatagtt cacaaaatcc attgttaaac ttcacgaaag tggtaaaact caaaatgctt
    24781 tatataggat tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa
    24841 gtaaaaattg acgatgacac gttccttacg gctcatcaga tgaaacagct acaaaaacga
    24901 aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat tgccatattc acgcctcgct
    24961 cgacgaacta ttaaaggtca agatttacct gaagatcatc ctttttagga aattgaagct
    25021 ttttatgttg cacaatgcat ttataatact acaatgatgt tttcaccaga tattcttcta
    25081 gggtgcggtg taatgaaaca gatacacttg caaaagaaaa ttcaccgcga atttactaaa
    25141 attatgaata attatgttaa tcttccgaaa tttttttcca aaattaaggg atcaagctgg
    25201 tgttatagat tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat tatagaaaac
    25261 cgatactctg ttaagttggt tgacacggtg tcgattttta tgactttctt attctgaaac
    25321 ttcactaacg gacagtacct aaaaagtcta tagtatggac gtttacgagt actattttat
    25381 caaaaatgct gctcatttca acttatatgt tttagtgaac tacccccact tagcttggat
    25441 aggctacaat taactagaca gaaaaattaa ggtgtgtaga ctagaagaaa acataccagg
    25501 aggaattttt atgtctaaga gaacacgaag aactttttca caagaattca agcaacaaat
    25561 cgtcaatctt tacttagctg gaaagccacg tgtagaaatc attcgagaat atgaactaac
    25621 ggcttcagca tttgacaaat gggtaaagca atc
//
LOCUS       DASJUF010000032        25314 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJUF010000032 DASJUF010000000
VERSION     DASJUF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.032"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            227..1867
                     /locus_tag="U4B62_001179"
     CDS             227..1867
                     /locus_tag="U4B62_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN2141350.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2021..3343
                     /locus_tag="U4B62_001180"
     CDS             2021..3343
                     /locus_tag="U4B62_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN2141351.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3405..3836)
                     /locus_tag="U4B62_001181"
     CDS             complement(3405..3836)
                     /locus_tag="U4B62_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2141352.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3900..4736)
                     /locus_tag="U4B62_001182"
     CDS             complement(3900..4736)
                     /locus_tag="U4B62_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN2141353.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4926..5228)
                     /locus_tag="U4B62_001183"
     CDS             complement(4926..5228)
                     /locus_tag="U4B62_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN2141354.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5254..5685)
                     /gene="ruvX"
                     /locus_tag="U4B62_001184"
     CDS             complement(5254..5685)
                     /gene="ruvX"
                     /locus_tag="U4B62_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN2141355.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5682..5948)
                     /locus_tag="U4B62_001185"
     CDS             complement(5682..5948)
                     /locus_tag="U4B62_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN2141356.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6202..6927)
                     /locus_tag="U4B62_001186"
     CDS             complement(6202..6927)
                     /locus_tag="U4B62_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN2141357.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7293..7363)
                     /locus_tag="U4B62_001187"
     tRNA            complement(7293..7363)
                     /locus_tag="U4B62_001187"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7329..7331),aa:Gly,seq:ccc)
     gene            7480..8232
                     /locus_tag="U4B62_001188"
     CDS             7480..8232
                     /locus_tag="U4B62_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2141358.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8248..8727
                     /locus_tag="U4B62_001189"
     CDS             8248..8727
                     /locus_tag="U4B62_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2141359.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8978..10558)
                     /locus_tag="U4B62_001190"
     CDS             complement(8978..10558)
                     /locus_tag="U4B62_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN2141360.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10551..11294)
                     /locus_tag="U4B62_001191"
     CDS             complement(10551..11294)
                     /locus_tag="U4B62_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141361.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11470..12246)
                     /locus_tag="U4B62_001192"
     CDS             complement(11470..12246)
                     /locus_tag="U4B62_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2141362.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12494..13330)
                     /locus_tag="U4B62_001193"
     CDS             complement(12494..13330)
                     /locus_tag="U4B62_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN2141363.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13341..14270)
                     /locus_tag="U4B62_001194"
     CDS             complement(13341..14270)
                     /locus_tag="U4B62_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN2141364.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14462..14815
                     /locus_tag="U4B62_001195"
     CDS             14462..14815
                     /locus_tag="U4B62_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2141365.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14889..15545)
                     /locus_tag="U4B62_001196"
     CDS             complement(14889..15545)
                     /locus_tag="U4B62_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2141366.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15771..17705
                     /locus_tag="U4B62_001197"
     CDS             15771..17705
                     /locus_tag="U4B62_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2141367.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17791..19014)
                     /locus_tag="U4B62_001198"
     CDS             complement(17791..19014)
                     /locus_tag="U4B62_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2141368.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19008..20906)
                     /locus_tag="U4B62_001199"
     CDS             complement(19008..20906)
                     /locus_tag="U4B62_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN2141369.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20900..22018)
                     /locus_tag="U4B62_001200"
     CDS             complement(20900..22018)
                     /locus_tag="U4B62_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2141370.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22101..22934)
                     /locus_tag="U4B62_001201"
     CDS             complement(22101..22934)
                     /locus_tag="U4B62_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2141371.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22927..23844)
                     /locus_tag="U4B62_001202"
     CDS             complement(22927..23844)
                     /locus_tag="U4B62_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2141372.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24071..25195)
                     /locus_tag="U4B62_001203"
     CDS             complement(24071..25195)
                     /locus_tag="U4B62_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2141373.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7806 a         5047 c         4308 g         8153 t
ORIGIN      
        1 ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag agatacgcat
       61 agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt gatttctttt
      121 gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact atatcctcat
      181 ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc ttacagggct
      241 aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag aagaacgggc
      301 aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac aacaatctgt
      361 ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta tcttcttaat
      421 gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac atttagttga
      481 attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat acaacgaaat
      541 tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa atatcaaagc
      601 aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga cagatgcaga
      661 aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta tcggtaaatt
      721 gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg tatcaatcga
      781 cgctggtact aataacgaag aattattgaa taatccatta tacttaggga atcgtcatgc
      841 tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat cagctactga
      901 attattccca gaattgttat tacattggga agattttgga cgaggaaatg ctgcaacgat
      961 cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag gcacaggaat
     1021 cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat tgaaagatca
     1081 aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca tccttttaga
     1141 tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct atcaagtaga
     1201 taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa tcccattcgc
     1261 tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag cagttgtaaa
     1321 agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat tcactgaaga
     1381 aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt tgtctaatcc
     1441 aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg gaaaagcttt
     1501 agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt accaaatcgg
     1561 tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg cttcaacagc
     1621 tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg gaatcgttga
     1681 tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg aattctctca
     1741 aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc gtgaagaaat
     1801 cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata aatctttaga
     1861 agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc taaaaagaaa
     1921 aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc ttctctttgg
     1981 cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa aaacaacaac
     2041 cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa ttaccggcgt
     2101 aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg ctttaggaaa
     2161 attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta atttatttca
     2221 ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct ccgtttttgc
     2281 gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg tagtaaaatc
     2341 cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg ccttgattac
     2401 tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc gttttattcc
     2461 cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag gtatgcttat
     2521 cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg ctggtggtgt
     2581 tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta cggacgcttc
     2641 ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag ctatcattgg
     2701 tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta atggtaaatt
     2761 aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt ccattccaca
     2821 attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa tctgtaacta
     2881 ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta tctgtaaaat
     2941 cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca acttgattgt
     3001 caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga atctaaatgt
     3061 attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg ttatcgcgat
     3121 ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg aatcaactat
     3181 tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg tcttatctgc
     3241 agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg gtggtgccat
     3301 cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa aaatacctga
     3361 tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag gattttcaaa
     3421 aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg gtgtgcttct
     3481 actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt cgcttcttct
     3541 acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga cacagcaagt
     3601 gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc ttgtacatag
     3661 ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt ttctggctcc
     3721 ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata tcctaattgt
     3781 tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg caacatccaa
     3841 atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata ctcctttttt
     3901 cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc ttctaatgtt
     3961 tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac aatcgcttga
     4021 aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc ccacaaacct
     4081 ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg cataaagcta
     4141 cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt agatagagaa
     4201 tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga aacattgact
     4261 ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc acatataagg
     4321 taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta gaagaacgca
     4381 aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt ctcgttttca
     4441 aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac aacagctcca
     4501 ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac ttgtcgcaca
     4561 gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat ccctgcaaca
     4621 gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc catcccggaa
     4681 atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc aatcatcttg
     4741 tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt tattatacag
     4801 taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt tagtgctaaa
     4861 cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt atcgattctt
     4921 ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat atcccattct
     4981 gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc gatgtatgca
     5041 taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa aagaacatag
     5101 ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa tgtttcattt
     5161 ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga atggttatgt
     5221 tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac ctgagccgtc
     5281 taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt ttcgtttcgc
     5341 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttagtcgttc
     5401 atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat atgacatgga
     5461 agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa ctacaaaacg
     5521 gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact cttcttctgc
     5581 ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat cgctcacagc
     5641 aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
     5701 tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg acggcgaatc
     5761 aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag caagtagccg
     5821 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
     5881 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
     5941 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaccctt
     6001 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga ataaattaag
     6061 aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc ctctttccat
     6121 aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca taaagttcat
     6181 aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg tctcataatt
     6241 gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt ttttgtcttc
     6301 aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct cgaaatgagg
     6361 agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa tatcattttc
     6421 gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt ttttaatgtt
     6481 attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata accattgtcc
     6541 gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt ctctcttttc
     6601 agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt tcaatttgtc
     6661 tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt ttgtaaactc
     6721 cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg gcacatccgc
     6781 tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga agatatctga
     6841 atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga cgatccccac
     6901 gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt tccctaaatc
     6961 ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta aaaaaataaa
     7021 accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt ctctactcaa
     7081 tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct taatactcaa
     7141 aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg catctgcggt
     7201 aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt cttattactt
     7261 gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg aacccgcgac
     7321 atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa aaacagtata
     7381 cgcctctttc actccttcct caagtttttt tattctttac gcttccgata tgttttgtag
     7441 taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa agaattagat
     7501 ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa tgcggagatg
     7561 tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt ccgtttggtc
     7621 agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac catccgaatg
     7681 gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa tgatctttca
     7741 cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact gcaagcaatc
     7801 gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc agtgatcagt
     7861 ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac tttagtcatt
     7921 ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat caaattttta
     7981 tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt acgttttggc
     8041 ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt tcctggagtt
     8101 caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg agtttctcgc
     8161 ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt tattttccag
     8221 cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt ttcttttagt
     8281 tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc attgatttgt
     8341 tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca aataggcgga
     8401 tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag cgatcttttc
     8461 tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt tcctctagtc
     8521 cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta tcgagaagcg
     8581 gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg tcttgtatta
     8641 tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc aatcatgatg
     8701 aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt tgtgaaagag
     8761 acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt aaccgcagga
     8821 gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt ctcacatttt
     8881 tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat ctgtaaaaaa
     8941 ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt ttccaaaacg
     9001 ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg atcacaggaa
     9061 tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg ataagaataa
     9121 tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct gaacctctgt
     9181 taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct ctagcgaaat
     9241 aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta gggataaaaa
     9301 acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt aaaaaactct
     9361 gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag attggcaaag
     9421 aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata aaagaagtct
     9481 gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg attaacttat
     9541 tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata aaaatgattg
     9601 gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt actaattgag
     9661 cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt ctttttattt
     9721 tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc agctgtttta
     9781 cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat aaaccttgtt
     9841 gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc ggatgccgat
     9901 ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt ccagcaaatg
     9961 cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt tttttaaaca
    10021 ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga ataatcaaaa
    10081 atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga tcagctcgcc
    10141 atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg gtcaaagcaa
    10201 ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc aaaaatactt
    10261 gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga gttaaaccta
    10321 acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca aagttcatag
    10381 caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga ttttgagaca
    10441 aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta ttcgtgagct
    10501 gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc ttatacatac
    10561 ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc tagcgtttca
    10621 tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc acctttcttc
    10681 aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt agaaaaaatg
    10741 actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa tgctgcttga
    10801 ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg cagcaacgtg
    10861 ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt ttcattttga
    10921 tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt tttttgtgtg
    10981 tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa ttgtaaaaaa
    11041 atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg attttccgtc
    11101 aatttcattc catctactaa aatcgttccc gatgatggtt caataatact aactaaactt
    11161 ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat ttccccttgt
    11221 cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt acttacattg
    11281 atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg tacccttcgt
    11341 tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa cgactaggct
    11401 gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag atatgagtct
    11461 ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct ttagtttatc
    11521 aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta tttcgtttac
    11581 tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat ctttacataa
    11641 atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga gaatctcttc
    11701 atctttcaat actaggccta ttttattttt ataacctggc atgctcatta gataaaggat
    11761 ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc accttttttt
    11821 cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt ggatgctttt
    11881 tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa tctttcgatt
    11941 aataacagtt attgctttat tcggatgaga cgttagataa acaattttac acttttcatt
    12001 cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca aatcgatgtc
    12061 taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa caaattgtat
    12121 aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc tggattcttc
    12181 caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa atatacgcgt
    12241 taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat acaacttttc
    12301 aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac acctttgaaa
    12361 ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa atgaatacat
    12421 catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag ctatgtttat
    12481 ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca gagactttct
    12541 cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa tagtctgatt
    12601 ggatggttcc ttctatttta taccattcat catttttaac cgttactcct tccggcatat
    12661 gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg aatcggaaca
    12721 agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca tagctgattt
    12781 ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct aaatagtttt
    12841 catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca ttcatttggt
    12901 ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat tgtgtctgca
    12961 tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg gcttctacga
    13021 tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc aaagctagca
    13081 gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga tcgtgctcgt
    13141 gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact aaagccaaaa
    13201 gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg atatattgat
    13261 cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat aaaatcaaaa
    13321 accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct aagggcaatc
    13381 agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat cagtagattt
    13441 ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt gccgaacaga
    13501 cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat cgtaaaggcc
    13561 aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag cataattcta
    13621 gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa ataacgacct
    13681 gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg gatcagctga
    13741 ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg gtgattttct
    13801 gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata ggcaagccag
    13861 acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc catctgccaa
    13921 gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat cggagcagtc
    13981 agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg atgaacgatc
    14041 ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc agtaaaaata
    14101 gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa aaatacttgt
    14161 aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat aacgatcgat
    14221 aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat gagtttcctc
    14281 ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa aaaacaagtt
    14341 gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt gactttttct
    14401 gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag ggagaataaa
    14461 aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt catccgttgc
    14521 cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc cacgactaga
    14581 ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg aaacaccaga
    14641 agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc atcaaggaaa
    14701 agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc tactagatga
    14761 actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa aataatcgtt
    14821 gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg ttcaatcgct
    14881 ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg caagaaatag
    14941 atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc tgacggagca
    15001 ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt taactgattg
    15061 actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat ttgttggatc
    15121 ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac gatgcgccca
    15181 acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact ttgatggaat
    15241 ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac atttgtcaat
    15301 tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc ataaccatac
    15361 ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc actatctact
    15421 tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt atttaaaatc
    15481 cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat aatatgatct
    15541 ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca tcatagcagt
    15601 atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt ctttattcat
    15661 ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac tactaattga
    15721 aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc atgatattat
    15781 tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa aacatacatt
    15841 tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct ggaaaatcga
    15901 ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc gctaaaaata
    15961 aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat gaaacagttt
    16021 ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg atgcgcgagc
    16081 tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct tatttaaaag
    16141 aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat gaaaatgaaa
    16201 ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag aaaatcgata
    16261 ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg caacaccctg
    16321 acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt tcctggcttg
    16381 agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac cgttattttt
    16441 tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc cattacaaag
    16501 gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa tggaaggctt
    16561 atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg aaccttgttc
    16621 gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa atggaccggc
    16681 tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata gaaaagcctt
    16741 ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat gtcaccttat
    16801 ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta ggtccgaatg
    16861 gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt atccgtggaa
    16921 aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa gctgatctgc
    16981 atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact ccagaaaaag
    17041 agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa aaaccaatcc
    17101 ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg atgaacaaag
    17161 aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac aaagaagtat
    17221 tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat gaccgatatt
    17281 tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc aaagtctatc
    17341 taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc gccgaattgt
    17401 tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat tattaccaaa
    17461 gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag gttgaagaag
    17521 aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc gcgccagaaa
    17581 acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct gctagacaag
    17641 atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa gaatatgaaa
    17701 attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct ttttttattt
    17761 tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat tactagtcat
    17821 tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat ttaccccatt
    17881 atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt ccgttgtagc
    17941 atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata agttcttttc
    18001 tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca tgatcaccat
    18061 actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc aataacaaat
    18121 ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta aatcaaaata
    18181 tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc atgcttggcc
    18241 tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc ttggttttcc
    18301 atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta ctaaataatc
    18361 tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc cagtttcttc
    18421 actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc taccttgttg
    18481 tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc aaaaacgctg
    18541 aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc ccgttatttt
    18601 ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat tagtgataat
    18661 ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt gttgaaataa
    18721 aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc cttctgtcca
    18781 accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg ggaattgata
    18841 ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac gctcgatctc
    18901 attattccat tctttttcca ttaattcttt ataacattca tttatccatt tttctggatg
    18961 tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta gcataatttt
    19021 agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc aaaatcaatt
    19081 ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc aaccactcct
    19141 gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat aaaagtgttt
    19201 tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt tcgtttaata
    19261 gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct atagttttcc
    19321 ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc aaaccctgtc
    19381 tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg ccagccttca
    19441 aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt agaggcaagt
    19501 acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg tccataggtt
    19561 ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg aatccctagc
    19621 attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga aaaaccaaat
    19681 tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa atttggccct
    19741 aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag caaattggct
    19801 tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc cgtccaatac
    19861 tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa tggagataac
    19921 gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat attcaggata
    19981 cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt gactaacccc
    20041 ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga ccaaaagctt
    20101 gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc atcttccgca
    20161 aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat ctggctaatg
    20221 ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc aaaaggatta
    20281 taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag ataacaattt
    20341 tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc aaatttttgg
    20401 ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca ttcatattca
    20461 aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa ttttttccat
    20521 aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac catacgctgg
    20581 tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc agcttttatt
    20641 cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg ccccgctccc
    20701 cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc taccccttga
    20761 tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg ttgatttatc
    20821 tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg atgtttcatt
    20881 gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat cgaagtaagc
    20941 cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt tctctatggt
    21001 atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt gcatagtgct
    21061 tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat cgtacagagg
    21121 aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca ctggtatcaa
    21181 tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt tcgattttct
    21241 gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt tttgtgtaac
    21301 gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa caagaattct
    21361 cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca tcaaattctg
    21421 cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta tctgcatgct
    21481 gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa tacagtaatg
    21541 gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc catgctacta
    21601 aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt aaccctagtt
    21661 gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt aagaataaaa
    21721 aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat aattgttctt
    21781 gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc tgccataaaa
    21841 taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt acgtctgtat
    21901 attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat tccgcatcta
    21961 tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt tcattcatgg
    22021 atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac gaactagatg
    22081 gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc cttaaattta
    22141 gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa aattagcata
    22201 attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt gataattaag
    22261 ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata ttgattccaa
    22321 cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat cggtacagcg
    22381 attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc catcagtgct
    22441 gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact ggcaatcgaa
    22501 gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat gtaaaataaa
    22561 ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt gaacaacagt
    22621 ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga actggctgta
    22681 gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa gggataatta
    22741 tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt gaaattgatt
    22801 ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata taaatatggc
    22861 atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct gctttgtatc
    22921 ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata atataacaga
    22981 tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca tttcaaaact
    23041 tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat tggaagtccc
    23101 aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta aaactgtggc
    23161 cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc gaaatttcga
    23221 tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa gaccagctaa
    23281 aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca ttgatattcg
    23341 tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat tcaaaaatcc
    23401 attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag ccaccgttga
    23461 tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta actgtgctga
    23521 attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg atcctgccat
    23581 gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga tagctagata
    23641 attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa aagcaaacga
    23701 gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg tgaccaatcc
    23761 ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct ttattgtctt
    23821 gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa tcaccagtta
    23881 attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa ttttcaaaat
    23941 cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca tctgaataag
    24001 tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt tcatagtatt
    24061 tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag gccaatgagt
    24121 tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct gtcagcataa
    24181 atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca taaaaacttg
    24241 atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc agtggagcca
    24301 tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca taccagccgc
    24361 cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca ccgtagaaat
    24421 gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc gcttctttag
    24481 tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg gctagcatgt
    24541 tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta ccagtttttt
    24601 cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct tcaaaatcta
    24661 cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta aaatcactag
    24721 cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat ttttgatcat
    24781 aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa aaagcatctt
    24841 gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata tccggcatat
    24901 cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc gaagctacat
    24961 tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt gcagcatttt
    25021 caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg gcttttttta
    25081 tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat gcacttaagc
    25141 tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt ttcatgtcta
    25201 tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata ttaacggaat
    25261 tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct tttc
//
LOCUS       DASJUF010000033        25223 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJUF010000033 DASJUF010000000
VERSION     DASJUF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.033"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..388)
                     /locus_tag="U4B62_001204"
     CDS             complement(164..388)
                     /locus_tag="U4B62_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN2141374.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="U4B62_001205"
     CDS             complement(586..2166)
                     /locus_tag="U4B62_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN2141375.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="U4B62_001206"
     CDS             complement(2567..3943)
                     /locus_tag="U4B62_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2141376.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="U4B62_001207"
     CDS             complement(4071..5189)
                     /locus_tag="U4B62_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2141377.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="U4B62_001208"
     CDS             5331..5765
                     /locus_tag="U4B62_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2141378.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="U4B62_001209"
     CDS             complement(5843..6376)
                     /locus_tag="U4B62_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN2141379.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U4B62_001210"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U4B62_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN2141380.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U4B62_001211"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U4B62_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN2141381.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="U4B62_001212"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="U4B62_001212"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2141382.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="U4B62_001213"
     CDS             complement(10101..10679)
                     /locus_tag="U4B62_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN2141383.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="U4B62_001214"
     CDS             11001..11471
                     /locus_tag="U4B62_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN2141384.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="U4B62_001215"
     CDS             complement(11579..12805)
                     /locus_tag="U4B62_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2141385.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="U4B62_001216"
     CDS             complement(13559..13759)
                     /locus_tag="U4B62_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2141386.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="U4B62_001217"
     CDS             complement(13893..14501)
                     /locus_tag="U4B62_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN2141387.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="U4B62_001218"
     CDS             complement(14586..15056)
                     /locus_tag="U4B62_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN2141388.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="U4B62_001219"
     CDS             complement(15104..16018)
                     /locus_tag="U4B62_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2141389.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="U4B62_001220"
     CDS             16164..16967
                     /locus_tag="U4B62_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN2141390.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="U4B62_001221"
     CDS             17145..18092
                     /locus_tag="U4B62_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN2141391.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="U4B62_001222"
     CDS             complement(18160..18453)
                     /locus_tag="U4B62_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN2141392.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="U4B62_001223"
     CDS             complement(18481..19146)
                     /locus_tag="U4B62_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN2141393.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="U4B62_001224"
     CDS             complement(19286..19918)
                     /locus_tag="U4B62_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2141394.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /locus_tag="U4B62_001225"
     CDS             complement(19925..20992)
                     /locus_tag="U4B62_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2141395.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /locus_tag="U4B62_001226"
     CDS             complement(20989..21720)
                     /locus_tag="U4B62_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN2141396.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="U4B62_001227"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="U4B62_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN2141397.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="U4B62_001228"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="U4B62_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN2141398.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="U4B62_001229"
     CDS             23883..25052
                     /locus_tag="U4B62_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2141399.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         7577 a         5089 c         4061 g         8496 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caa
//
LOCUS       DASJUF010000034        24551 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJUF010000034 DASJUF010000000
VERSION     DASJUF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.034"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U4B62_001230"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U4B62_001230"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2141400.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="U4B62_001231"
     CDS             complement(937..1518)
                     /locus_tag="U4B62_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2141401.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U4B62_001232"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U4B62_001232"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN2141402.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="U4B62_001233"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="U4B62_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN2141403.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="U4B62_001234"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="U4B62_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN2141404.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="U4B62_001235"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="U4B62_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN2141405.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="U4B62_001236"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="U4B62_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2141406.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="U4B62_001237"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="U4B62_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN2141407.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="U4B62_001238"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="U4B62_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN2141408.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="U4B62_001239"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="U4B62_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN2141409.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="U4B62_001240"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="U4B62_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN2141410.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="U4B62_001241"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="U4B62_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN2141411.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="U4B62_001242"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="U4B62_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN2141412.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="U4B62_001243"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="U4B62_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN2141413.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="U4B62_001244"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="U4B62_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN2141414.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="U4B62_001245"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="U4B62_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN2141415.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="U4B62_001246"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="U4B62_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN2141416.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="U4B62_001247"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="U4B62_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN2141417.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="U4B62_001248"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="U4B62_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN2141418.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="U4B62_001249"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="U4B62_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN2141419.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="U4B62_001250"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="U4B62_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN2141420.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="U4B62_001251"
     CDS             13972..14157
                     /locus_tag="U4B62_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN2141421.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="U4B62_001252"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="U4B62_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN2141422.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="U4B62_001253"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="U4B62_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN2141423.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="U4B62_001254"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="U4B62_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN2141424.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="U4B62_001255"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="U4B62_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN2141425.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="U4B62_001256"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="U4B62_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN2141426.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="U4B62_001257"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="U4B62_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN2141427.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="U4B62_001258"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="U4B62_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN2141428.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="U4B62_001259"
     CDS             18232..18879
                     /locus_tag="U4B62_001259"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN2141429.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="U4B62_001260"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="U4B62_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN2141430.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="U4B62_001261"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="U4B62_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN2141431.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="U4B62_001262"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="U4B62_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN2141432.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="U4B62_001263"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="U4B62_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN2141433.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="U4B62_001264"
     CDS             20317..21255
                     /locus_tag="U4B62_001264"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN2141434.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="U4B62_001265"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="U4B62_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN2141435.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="U4B62_001266"
     CDS             21935..22774
                     /locus_tag="U4B62_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2141436.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="U4B62_001267"
     CDS             22750..23619
                     /locus_tag="U4B62_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2141437.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="U4B62_001268"
     CDS             23609..24406
                     /locus_tag="U4B62_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2141438.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT         7953 a         4548 c         5279 g         6771 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa a
//
LOCUS       DASJUF010000035        24381 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJUF010000035 DASJUF010000000
VERSION     DASJUF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.035"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..829)
                     /locus_tag="U4B62_001269"
     CDS             complement(<1..829)
                     /locus_tag="U4B62_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2141439.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     "
     gene            complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="U4B62_001270"
     CDS             complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="U4B62_001270"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN2141440.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3330..3509)
                     /locus_tag="U4B62_001271"
     CDS             complement(3330..3509)
                     /locus_tag="U4B62_001271"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN2141441.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3556..5379)
                     /gene="cadA"
                     /locus_tag="U4B62_001272"
     CDS             complement(3556..5379)
                     /gene="cadA"
                     /locus_tag="U4B62_001272"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2141442.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5657..7936
                     /locus_tag="U4B62_001273"
     CDS             5657..7936
                     /locus_tag="U4B62_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2141443.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8029..8676)
                     /locus_tag="U4B62_001274"
     CDS             complement(8029..8676)
                     /locus_tag="U4B62_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141444.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8855..9574)
                     /locus_tag="U4B62_001275"
     CDS             complement(8855..9574)
                     /locus_tag="U4B62_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2141445.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9705..10856)
                     /locus_tag="U4B62_001276"
     CDS             complement(9705..10856)
                     /locus_tag="U4B62_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN2141446.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11073..11813)
                     /locus_tag="U4B62_001277"
     CDS             complement(11073..11813)
                     /locus_tag="U4B62_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2141447.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11817..12140)
                     /gene="rsfS"
                     /locus_tag="U4B62_001278"
     CDS             complement(11817..12140)
                     /gene="rsfS"
                     /locus_tag="U4B62_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN2141448.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12197..12790)
                     /gene="yqeK"
                     /locus_tag="U4B62_001279"
     CDS             complement(12197..12790)
                     /gene="yqeK"
                     /locus_tag="U4B62_001279"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN2141449.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12774..13424)
                     /locus_tag="U4B62_001280"
     CDS             complement(12774..13424)
                     /locus_tag="U4B62_001280"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN2141450.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="U4B62_001281"
     CDS             complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="U4B62_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN2141451.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="U4B62_001282"
     CDS             complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="U4B62_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN2141452.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14867..15394)
                     /locus_tag="U4B62_001283"
     CDS             complement(14867..15394)
                     /locus_tag="U4B62_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN2141453.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15574..16359)
                     /locus_tag="U4B62_001284"
     CDS             complement(15574..16359)
                     /locus_tag="U4B62_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN2141454.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16352..17221)
                     /locus_tag="U4B62_001285"
     CDS             complement(16352..17221)
                     /locus_tag="U4B62_001285"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN2141455.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17222..18601)
                     /locus_tag="U4B62_001286"
     CDS             complement(17222..18601)
                     /locus_tag="U4B62_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN2141456.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18609..19028)
                     /gene="fabZ"
                     /locus_tag="U4B62_001287"
     CDS             complement(18609..19028)
                     /gene="fabZ"
                     /locus_tag="U4B62_001287"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2141457.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19053..19529)
                     /gene="accB"
                     /locus_tag="U4B62_001288"
     CDS             complement(19053..19529)
                     /gene="accB"
                     /locus_tag="U4B62_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN2141458.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19532..20767)
                     /gene="fabF"
                     /locus_tag="U4B62_001289"
     CDS             complement(19532..20767)
                     /gene="fabF"
                     /locus_tag="U4B62_001289"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN2141459.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20786..21523)
                     /gene="fabG"
                     /locus_tag="U4B62_001290"
     CDS             complement(20786..21523)
                     /gene="fabG"
                     /locus_tag="U4B62_001290"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN2141460.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21535..22452)
                     /gene="fabD"
                     /locus_tag="U4B62_001291"
     CDS             complement(21535..22452)
                     /gene="fabD"
                     /locus_tag="U4B62_001291"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2141461.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22559..22783)
                     /locus_tag="U4B62_001292"
     CDS             complement(22559..22783)
                     /locus_tag="U4B62_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2141462.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22836..23798)
                     /locus_tag="U4B62_001293"
     CDS             complement(22836..23798)
                     /locus_tag="U4B62_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN2141463.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23808..24248)
                     /locus_tag="U4B62_001294"
     CDS             complement(23808..24248)
                     /locus_tag="U4B62_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2141464.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6663 a         5431 c         4246 g         8041 t
ORIGIN      
        1 attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca
       61 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
      121 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      181 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      241 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      301 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      361 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      421 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      481 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      541 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      601 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      661 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      721 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      781 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      841 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
      901 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
      961 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
     1021 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1081 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1141 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1201 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1261 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1321 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1381 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1441 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1501 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1561 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1621 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1681 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1741 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1801 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1861 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     1921 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     1981 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     2041 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2101 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2161 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2221 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2281 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2341 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2401 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2461 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2521 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2581 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2641 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2701 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2761 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2821 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     2881 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     2941 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     3001 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3061 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3121 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3181 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3241 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3301 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3361 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3421 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3481 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3541 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3601 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3661 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3721 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3781 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3841 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     3901 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     3961 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     4021 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4081 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4141 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4201 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4261 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4321 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4381 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4441 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4501 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4561 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4621 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4681 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4741 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4801 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4861 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     4921 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     4981 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     5041 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5101 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5161 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5221 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5281 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5341 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5401 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5461 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5521 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5581 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5641 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5701 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5761 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5821 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     5881 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     5941 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     6001 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6061 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6121 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6181 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6241 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6301 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6361 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6421 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6481 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6541 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6601 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6661 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6721 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6781 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6841 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     6901 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     6961 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     7021 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7081 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7141 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7201 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7261 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7321 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7381 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7441 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7501 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7561 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7621 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7681 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7741 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7801 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7861 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     7921 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     7981 atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata
     8041 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
     8101 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
     8161 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
     8221 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
     8281 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
     8341 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
     8401 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
     8461 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
     8521 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
     8581 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
     8641 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
     8701 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
     8761 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
     8821 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
     8881 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
     8941 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
     9001 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     9061 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     9121 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     9181 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     9241 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     9301 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     9361 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     9421 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     9481 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     9541 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     9601 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     9661 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     9721 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     9781 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     9841 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     9901 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
     9961 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
    10021 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
    10081 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
    10141 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
    10201 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
    10261 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
    10321 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
    10381 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
    10441 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
    10501 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
    10561 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
    10621 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
    10681 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
    10741 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
    10801 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
    10861 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
    10921 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
    10981 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
    11041 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
    11101 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
    11161 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
    11221 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
    11281 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
    11341 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
    11401 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
    11461 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
    11521 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
    11581 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
    11641 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
    11701 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
    11761 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
    11821 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
    11881 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
    11941 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
    12001 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
    12061 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
    12121 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
    12181 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
    12241 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
    12301 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
    12361 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
    12421 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
    12481 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
    12541 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
    12601 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
    12661 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
    12721 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
    12781 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
    12841 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
    12901 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
    12961 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
    13021 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
    13081 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
    13141 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
    13201 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
    13261 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
    13321 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
    13381 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
    13441 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
    13501 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
    13561 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
    13621 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
    13681 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
    13741 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
    13801 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
    13861 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
    13921 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
    13981 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
    14041 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
    14101 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
    14161 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
    14221 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
    14281 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
    14341 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
    14401 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
    14461 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
    14521 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
    14581 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
    14641 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
    14701 gagacatctt gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
    14761 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
    14821 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
    14881 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
    14941 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
    15001 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
    15061 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
    15121 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
    15181 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
    15241 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
    15301 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
    15361 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
    15421 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
    15481 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
    15541 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
    15601 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
    15661 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
    15721 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
    15781 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
    15841 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
    15901 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
    15961 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
    16021 gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
    16081 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
    16141 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
    16201 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
    16261 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
    16321 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
    16381 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
    16441 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
    16501 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
    16561 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
    16621 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
    16681 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
    16741 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
    16801 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
    16861 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
    16921 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
    16981 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
    17041 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
    17101 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
    17161 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
    17221 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
    17281 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
    17341 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
    17401 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
    17461 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
    17521 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
    17581 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
    17641 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
    17701 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
    17761 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
    17821 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
    17881 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
    17941 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
    18001 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    18061 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    18121 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    18181 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    18241 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    18301 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    18361 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    18421 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    18481 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    18541 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    18601 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    18661 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    18721 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    18781 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    18841 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    18901 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    18961 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    19021 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    19081 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    19141 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    19201 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    19261 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    19321 attatttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc
    19381 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    19441 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    19501 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    19561 ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt
    19621 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    19681 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    19741 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    19801 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    19861 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    19921 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    19981 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    20041 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    20101 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    20161 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    20221 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    20281 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    20341 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    20401 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    20461 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    20521 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    20581 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    20641 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    20701 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    20761 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    20821 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    20881 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    20941 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    21001 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    21061 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    21121 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    21181 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    21241 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    21301 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    21361 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    21421 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    21481 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    21541 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    21601 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    21661 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    21721 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    21781 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    21841 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    21901 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    21961 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    22021 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    22081 tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    22141 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    22201 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    22261 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    22321 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    22381 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    22441 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    22501 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    22561 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    22621 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    22681 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    22741 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    22801 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    22861 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    22921 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    22981 tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc
    23041 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    23101 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    23161 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    23221 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    23281 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    23341 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    23401 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    23461 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    23521 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    23581 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    23641 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    23701 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    23761 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    23821 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    23881 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    23941 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    24001 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    24061 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    24121 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    24181 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    24241 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact
    24301 atatcttaac ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt
    24361 ttttaagatt ttactcattt a
//
LOCUS       DASJUF010000036        24162 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJUF010000036 DASJUF010000000
VERSION     DASJUF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.036"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..511)
                     /locus_tag="U4B62_001295"
     CDS             complement(<1..511)
                     /locus_tag="U4B62_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2141465.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALR"
     gene            complement(523..1215)
                     /locus_tag="U4B62_001296"
     CDS             complement(523..1215)
                     /locus_tag="U4B62_001296"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN2141466.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1234..1518)
                     /locus_tag="U4B62_001297"
     CDS             complement(1234..1518)
                     /locus_tag="U4B62_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141467.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1531..2094)
                     /locus_tag="U4B62_001298"
     CDS             complement(1531..2094)
                     /locus_tag="U4B62_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2141468.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2322..2984
                     /locus_tag="U4B62_001299"
     CDS             2322..2984
                     /locus_tag="U4B62_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN2141469.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3108..4304
                     /locus_tag="U4B62_001300"
     CDS             3108..4304
                     /locus_tag="U4B62_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN2141470.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4405..5781)
                     /locus_tag="U4B62_001301"
     CDS             complement(4405..5781)
                     /locus_tag="U4B62_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2141471.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5818..6684)
                     /locus_tag="U4B62_001302"
     CDS             complement(5818..6684)
                     /locus_tag="U4B62_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN2141472.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6773..7798)
                     /locus_tag="U4B62_001303"
     CDS             complement(6773..7798)
                     /locus_tag="U4B62_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2141473.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7816..8490)
                     /locus_tag="U4B62_001304"
     CDS             complement(7816..8490)
                     /locus_tag="U4B62_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2141474.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8674..9567)
                     /locus_tag="U4B62_001305"
     CDS             complement(8674..9567)
                     /locus_tag="U4B62_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2141475.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9641..11338)
                     /locus_tag="U4B62_001306"
     CDS             complement(9641..11338)
                     /locus_tag="U4B62_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2141476.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11331..12644)
                     /locus_tag="U4B62_001307"
     CDS             complement(11331..12644)
                     /locus_tag="U4B62_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2141477.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12644..16681)
                     /locus_tag="U4B62_001308"
     CDS             complement(12644..16681)
                     /locus_tag="U4B62_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2141478.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16694..17152)
                     /locus_tag="U4B62_001309"
     CDS             complement(16694..17152)
                     /locus_tag="U4B62_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2141479.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17450..17968)
                     /locus_tag="U4B62_001310"
     CDS             complement(17450..17968)
                     /locus_tag="U4B62_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2141480.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17970..18586)
                     /locus_tag="U4B62_001311"
                     /pseudo
     CDS             complement(17970..18586)
                     /locus_tag="U4B62_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(18583..19446)
                     /locus_tag="U4B62_001312"
     CDS             complement(18583..19446)
                     /locus_tag="U4B62_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2141481.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19433..20344)
                     /locus_tag="U4B62_001313"
     CDS             complement(19433..20344)
                     /locus_tag="U4B62_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2141482.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20385..21719)
                     /locus_tag="U4B62_001314"
     CDS             complement(20385..21719)
                     /locus_tag="U4B62_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2141483.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21927..22757
                     /locus_tag="U4B62_001315"
     CDS             21927..22757
                     /locus_tag="U4B62_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN2141484.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23001..23319)
                     /locus_tag="U4B62_001316"
                     /pseudo
     CDS             complement(23001..23319)
                     /locus_tag="U4B62_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23441..23635)
                     /locus_tag="U4B62_001317"
     CDS             complement(23441..23635)
                     /locus_tag="U4B62_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141485.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7096 a         5264 c         4191 g         7611 t
ORIGIN      
        1 gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggaatct ttgaaaatat
       61 ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac
      121 atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg
      181 ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat
      241 agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat
      301 tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa
      361 tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt
      421 tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat
      481 atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac
      541 aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct
      601 ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt
      661 gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg
      721 gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac
      781 gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg
      841 caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc
      901 aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac
      961 ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta
     1021 ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac
     1081 gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca
     1141 tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat
     1201 aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac
     1261 cacacataac attaggataa caataataag agatttcatc aaaaaagagt tccatttggt
     1321 cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt
     1381 acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg
     1441 tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat
     1501 aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta
     1561 ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc
     1621 ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt
     1681 ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg
     1741 tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc
     1801 taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc
     1861 tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg
     1921 atcttgttcg tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt
     1981 cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct
     2041 atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc
     2101 tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact
     2161 ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt
     2221 agtccgatac tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa
     2281 agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc
     2341 cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg
     2401 ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca
     2461 ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg
     2521 aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa
     2581 aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg
     2641 atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac
     2701 caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt
     2761 tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa
     2821 aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc
     2881 aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga
     2941 gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa
     3001 agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag
     3061 acattcatgt aagaatcgag aacagattca acaaggagga attttttatg gatcataaac
     3121 cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc
     3181 cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc
     3241 aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag
     3301 ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat
     3361 cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac
     3421 aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca
     3481 atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca
     3541 aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga
     3601 tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac
     3661 agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag
     3721 aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag
     3781 aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc
     3841 tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac
     3901 aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg
     3961 ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa
     4021 aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa
     4081 attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg
     4141 tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat
     4201 tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca
     4261 tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt
     4321 tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct
     4381 ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata
     4441 gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt
     4501 ttgcagacga agcatcaata caatacaagt caagatggag acgattggca atagcggaac
     4561 gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa
     4621 gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac
     4681 attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt
     4741 tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt
     4801 gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag
     4861 cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat
     4921 tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat
     4981 cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg
     5041 cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa
     5101 gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa
     5161 tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat
     5221 accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga
     5281 cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt
     5341 ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa
     5401 accttgcata tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc
     5461 gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc
     5521 atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa
     5581 aatgacaacg attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt
     5641 ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat
     5701 gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga gattgatctc
     5761 tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta
     5821 aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat
     5881 aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt
     5941 agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac
     6001 tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca
     6061 cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc
     6121 gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt
     6181 atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag
     6241 cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc
     6301 gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc
     6361 atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg
     6421 tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact
     6481 ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac
     6541 aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg
     6601 attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg
     6661 gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt
     6721 atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt
     6781 tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag
     6841 ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc
     6901 ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa
     6961 agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga
     7021 ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga
     7081 tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag
     7141 tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac
     7201 tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg
     7261 agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata
     7321 attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta
     7381 gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt
     7441 tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct
     7501 gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt
     7561 gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt
     7621 aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg
     7681 tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa
     7741 aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct
     7801 taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc
     7861 ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt
     7921 gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat
     7981 ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat
     8041 gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct
     8101 catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt
     8161 tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat
     8221 agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga
     8281 tacttcacgt attttttgac accaataata gccatccaaa acgggaagat taatatctag
     8341 taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt
     8401 tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc
     8461 ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac
     8521 tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc
     8581 aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt
     8641 aaatttatta tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt
     8701 ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc
     8761 tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga
     8821 gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt
     8881 gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt
     8941 tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata
     9001 cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt
     9061 ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa
     9121 agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa
     9181 taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta
     9241 atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg
     9301 tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc
     9361 taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat
     9421 ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc
     9481 agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg
     9541 aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc
     9601 aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt
     9661 tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg
     9721 ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt
     9781 ctggccctgg attttctact cctacgatct gaatttcttc attatagctt ccataagaga
     9841 tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg
     9901 tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat
     9961 ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt
    10021 cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat
    10081 tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa
    10141 actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt
    10201 catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt
    10261 ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc
    10321 tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc
    10381 cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc
    10441 gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt
    10501 aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg
    10561 ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt
    10621 gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct
    10681 gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt
    10741 ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca
    10801 taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa
    10861 gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg
    10921 cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc
    10981 aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt
    11041 ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa
    11101 acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc
    11161 tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt
    11221 cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta
    11281 ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt
    11341 ttttctcccc gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt
    11401 aaagaaatca acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga
    11461 cctggattgc ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg
    11521 ttataaacac gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat
    11581 tcagccatct cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa
    11641 aattcaggga tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca
    11701 ccttttaatt cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca
    11761 aggccctctt tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct
    11821 gctggtttga tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc
    11881 cccccttcta tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca
    11941 gttgttcctc tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt
    12001 ttaggataga tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc
    12061 aaaatagcag caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga
    12121 tcaatcgatt cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct
    12181 ttgatacgtg caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa
    12241 gtccctattt ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt
    12301 tcagcgattt gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga
    12361 taataatcgg tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa
    12421 atgatatctg ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat
    12481 tcaaattgat cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg
    12541 acatcttccc ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt
    12601 agtaaaacaa gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct
    12661 ttttagattg tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag
    12721 caagcaggat atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat
    12781 cgttcccgac actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact
    12841 cttgctgtag atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta
    12901 gtgtacccgc ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat
    12961 aactaatttc taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt
    13021 ccttgatccg atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg
    13081 gatctggtgc caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa
    13141 ttgaaaattc tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca
    13201 aatcgattgg cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat
    13261 agccttccgg caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac
    13321 cttcttcatt cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga
    13381 cagattccga gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat
    13441 cgacatactt ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt
    13501 tcgattcttc tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat
    13561 cccccacttt cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt
    13621 ttctaactcg gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac
    13681 gatcctcttc atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga
    13741 gttggatagt ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag
    13801 gttctttgaa accttctggg ttctggctct caatgatttt gtattctgca tagttggtag
    13861 tttcttcact atccttataa acaaatgaat ctggctctcc gattggatga ataactttct
    13921 caccatccac gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt
    13981 tctcttcctc tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca
    14041 gtccgttacc atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc
    14101 cttcaattag tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt
    14161 tgacatacaa ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct
    14221 tgtgatattc tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg
    14281 aaccagatgt atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg
    14341 catatttttt gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag
    14401 cgatctctct gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca
    14461 tcttcgtttc aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg
    14521 cttccccact ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc
    14581 cattttgacc cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag
    14641 aagatgggaa atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa
    14701 tataggccat attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta
    14761 cttggtactt gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag
    14821 cttggttttc ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc
    14881 catgacgttc ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat
    14941 ctagatggta aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac
    15001 cagtttcatc ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct
    15061 ccaaggctgt aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat
    15121 cttcttctaa atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc
    15181 tcccgctaac gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt
    15241 gatacttgtt tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt
    15301 gcgaagtagg actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg
    15361 ctttattatc tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg
    15421 gacggttaaa gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc
    15481 cagaaaactc ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca
    15541 tcacagtcca gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg
    15601 cataactgaa ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat
    15661 tttctccgcc attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg
    15721 gagttcctcc gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga
    15781 tatcatttgt caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac
    15841 tattatggta aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat
    15901 ctttactcat ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc
    15961 cagcggttaa ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac
    16021 tgatttcctt tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat
    16081 cttcaggatg tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga
    16141 tgtacgcccc gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct
    16201 gaacggtttg agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag
    16261 catagtcgta atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt
    16321 tctctccgtc atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat
    16381 ttcctaacac ttttgtatag tcattcgttg taaacttcgg gtagcgatgc tgttcgtttt
    16441 caacttctcc gctaggatag atatattcat aggggtcttg cgtagctgtt ggcccaagac
    16501 gctccaaaat accgttgaat aactggcgtt gaatgcgctg gagtgcttga ccgctgggta
    16561 cgattggttc gtccagcttt tccatctcat tttcttcgct acgttgaatt tcagcggaat
    16621 cttctgtatc agtttcattt tcttgtaatt cttggttatc tattctttca tcaaccacca
    16681 cttgaagagg tagttaccct tcgttcacat cggggatctt ttctgtttca aaaatcaatg
    16741 tgccggtttc tgcttctttt gaataaactt ttccgacata ccattcttga ccatcgatcg
    16801 tctcgacttt taggtcttct ccatcttcat cataggagga tagacctgtt ccacgaacgt
    16861 tttttacggt accaatcccg gcggctgccg catcgattgc taaacgtatt ctaccttcat
    16921 tggcactttc ctttttttca aaagccagat gccattcaag ggtttcttct ttttcaatat
    16981 acttcccttc aatttttgca tagtcctcat ctaagaatac ctcatcctcc tttgtttctg
    17041 ttgactcagc agctacatcc agggtcgtca tatgatagac tgactggaaa tatggtccaa
    17101 atagcaaact gacagttact aactgcaaaa gcttcaattt taaccctttc acatccttat
    17161 actcctttca aaaatatttg tcttctttca caacaatcaa caaaacaatg cccatctaat
    17221 tataatttaa tttaatttta attttaaatc aaatgatatc ctttttagtt aaaaaacacc
    17281 aattatacaa catttattat taaaattaaa ttataaaatt ttataacgtt ctcatttaac
    17341 aaaacttaag ttattaagac aaatgaataa acaactacgc tttcctcgaa acaaaaaaat
    17401 aaagcacaaa tgcgcattta cgcactcatg ctttattcgt acaattaatt cagataacgg
    17461 cagcgcatag gatgccaata acacttccta aaatagctcc tcctaaaatg tcagtaggat
    17521 aatgtaaaag taaaaaaatc ctcgaataag cgatcaaaca agcaaataaa aaggctaata
    17581 ttcctgcaac tggacttaat tcgaacaacg cagctactga taaaaaggct agcgctgtat
    17641 gtcctgaagg aaaagaagaa gtcttcggtt tacttgtgta aaggacaagc cgacgatttt
    17701 tttcataagg tcttttccgt cttgtcgttt ttttgataaa aagcaaacca acaaaaaaat
    17761 agatcaaact gccgataaga atggctagac catattctct agtatcaggt atcaagacta
    17821 aaacagcagc aaggagcaac caaataaaac caccattaga gatattactg ataaatttca
    17881 aaaaattgat ttttcgacta tccgttttcc gttgcttggc gaacttgttg ataatactat
    17941 catcgatttt agcaatcaaa cgcaacatgt tattcctcct ttttaagaac cttttcttgc
    18001 gtacgatgga taaaaagcag gacttgaaaa taaaacaaga aactgaaacc taataaagca
    18061 aaatagattg gtcctgcaaa ccagaatgcg acaagaagga tcaacgtgcc tgccaaatag
    18121 aaaagcgcgc ttttgaaatc attaaaaaac aagaaaaatg cagtaatcca agaagcttct
    18181 ttttgttcca gttgaagcaa gattttgtaa ctaaagagaa gcgcgcttaa tagaagcaaa
    18241 aacaacgtag tatacatcaa tgtatagcca attcccccac cccaatagcg gaaaatctga
    18301 taatcgatca atagaagaag aaaataaaca cttgctaaac tatcttgctt tccataaaga
    18361 gagaagttct cacgaaactt tttccagaat agtgaaccga gctttttctc ttcatttttt
    18421 tgtaatgata aatcatatac agaactgttc gacttgcagt tcctaatgtg aaaacaggca
    18481 agacgcacag gaaccatagt agctgtaaat ggataagacg tggaatccat tcaatgattt
    18541 ctaagaaata atcattggct tttttaccaa tcgatcgatt catcatcctt ttgaacctcc
    18601 tgtcaaatcc atcccttcaa tgaaataacg ttgtgcaaaa aagaacagca aaatcgtcgg
    18661 gatcaagaca agtgttgctc ctgccatcag ataattccat tgtgtcgttg cttggttctg
    18721 aaaaacttgc aagccaatct gtaacgtgta tttttcttga tcttggatat ataacagagg
    18781 acctaagaaa tcattccaag ctgcgttgaa ggaattgatc gcaatggtaa tcaaggcagg
    18841 tttcgtcaat ggaagcatca aatgactcca aatatacaaa tgattcgctc cgtcgatttc
    18901 agcagcttcg atcaattcat tattgatcga taagtaaaat tgacgcatca gaaatatatt
    18961 gaacgcattg ccgaagaacg atggaacgat caggggcaaa taagtaccta cccagccgat
    19021 cttggagaat aatacatatt gagggatcat cgttacaaac cccgggatca tcatcgtaga
    19081 aagaaccaaa gcaaacatca gctttttccc aggaaaatca agcttcgcaa atccataagc
    19141 aataaaagaa tttgaaagga cattaccgat cactactagc acagtgatga ataatgtatt
    19201 tcttgcatag cgccaaaaag gaaaggcggc aatcgtatcc agatagtttt cccatacgat
    19261 tttttctggg aaaaaggtcg gaggataacg aacgatttct tccattggtt tcaatgaagt
    19321 aaacaccatc caaagcaatg gaatgatcaa aatgattcct attactgtca tgaggacata
    19381 acttagtatt tttttgtttg ccgattcaca ctggtctttt tgaaataatc catcatttgc
    19441 cgtcacctcc agaataatag acccacttag gtgcaagctt caaattgaca aacgtcagaa
    19501 ccaacacgat cagaaacagg atcattgaca tagccatcgc ataacccata tcaaaagcta
    19561 cgaacgcctt gttccacata tgcagattat aaaaaagcaa agaatttgca ggtccgcctg
    19621 ttccattttt cgtcatgaca taagcttctt gaaaaatttg gaaagaaccg atcgtagagg
    19681 tgatcacatc gtaaaaaata ataggagtga ttaatggcaa agtaatactt ttgaactttc
    19741 cccaaacgcc tgctccatcg atttctgctg attcatataa agatctagga acattttgta
    19801 atgtagctaa gtaaagtagc attgctcctc cagaactcca tattttcatc aaaatcaacg
    19861 caggtttcgt ccattgtgga tcaaacagcc aagctggtcc agggatatgg aaccagccca
    19921 gaaatgtgtt gatcaatcct gcagatggag aaagcagctg catccataag atgtagactg
    19981 ctactcctga taagactgcc ggtaaataga aagctgtacg gaatacactg attcctttga
    20041 ttttttgatt gagtactgtg gctaaaaaca cactgaaaat cgtcgtcagc ggtacattga
    20101 agatgacgaa ataaacggtg ttgtataacg atttccaaaa taaatcatcg tgaacgaaca
    20161 ttcttttgaa atttgctaga ccaatgaaat tcatcttgga tgtcaaatta taatcggtga
    20221 aactaccaat aactgaaaaa agcataggcc ctagcataaa taccaagaac ccgataataa
    20281 aaggcgagat aaataaatag gcccatcttg cttctttttt ctttcggata gataaacgct
    20341 tcaaattttc ttgcctcctc tgtacctaga cagtcaaaaa tggattattg attttgcttg
    20401 actaagtttt ctactgcttt ttgtgcatct gccaatcctt cttttggtga ttttgttcct
    20461 aaaagaatct ggtcaatctg ttcattcact aatgtcgtgt aatccggagc atctaaagga
    20521 actggtgtga tcaccgtatt tttaaagttt tcgtccgcca tttgatagat catttttcct
    20581 ttatccgtta ggttgtcatt attcactaaa ttctcattgg ctgtcttgct cgccatgata
    20641 tcaaaacttt tttcgccgaa tttttcttgt acttctggag aagaaagata tttgatgaat
    20701 tcataagagg cctcaggatc ttttgctccg tatggtactt ccaagacgaa tccgccaccc
    20761 cacgaccaat ggcctgaacc ttcttctttt tctgggagct gcatcacgcc aaaatcaaaa
    20821 tcatccggtg cattttccat caaactagag taataattga tattttgggc acgcatggct
    20881 actaatccgg aaataaatgg atcagataca ccagacccaa attcggcttc taatttattg
    20941 atcgtatttt tcccataata atcttgccag tcttggatcc actccaaggc ttccacttta
    21001 tttttagtat caatcttgac tttttcatct ttgtcaaacc aactagtccc gttatctgcg
    21061 tttagcgccc atacatcagc ccctaaattc cataatggat aaaaaccgat gcgtgtaaat
    21121 tcttttcctt cttttttatc tagtttacgt gctgtttctt ctaattcatc ccaagttgtc
    21181 ggtagatctt gttcactgat tccagcttct ttaaatatct ttttattata aaaaatgact
    21241 tgtgtatctg tattgaatgg gatcgcataa ggatcacctt catattctac tgtatcccaa
    21301 agctgcggat aaaaatcctt agaagtgtct ttttctacat atttggagag attagttgct
    21361 tgctgtgctt ctgctctttg ggcaacggaa tttatgtcct gtacaataac atcgggtgca
    21421 tttccagctg ttattgcaga taaagattta gtccagatgt caccccatgg ctgatactta
    21481 taatttacat ggatctcgtt ttgtgattga ttgaaatcct caatgatttc ttcgatagct
    21541 tcttttcgtg caccggatcc ccaaaaggac cagaaatcaa tattttttct tccatcactt
    21601 gttttttcag aagaatttgc tgcacttgaa ctggcatcgc ttgcattact tgatccgcat
    21661 cctcctaata aaaacaatgc tgaacttgcc agcaaccctg taaacaataa ttttttcata
    21721 ttttacctcc aaattttaat gtaaccgatg ccggttttaa tataacgata tcattttgtt
    21781 ctgaaaataa caatttataa ggtatgattc taaaaaaatt attaatgata ccaatacttt
    21841 atataattcg cgagcgcttt ctttgaattt tttaatcttt cgtagcagaa tgagaatgta
    21901 ttaaaaatag attaggagga attttgatgt ttagacacca gaaagaattg cagtttgaag
    21961 caaaaccaga tcgtccgaat ccattaatcg cgaaatatct gcaggagctg atcggcggcc
    22021 aatatggcga gatgagtgta gctatgcagt acctgttcca aggctggagc tgccgaggag
    22081 aagaaaaata taaagatatg ctgatggaca ttgcaacaga agaaatcggc cacgtggaaa
    22141 tgttggcgac aatggttgct cgtttattag agggtgcgcc tgttgaagat caagaagcag
    22201 cgatgcaaaa agatccaaca atcggtgcca ttatctctgg aatgaatcca cagcacgcga
    22261 tcgtagctgg cttaggacct cgtcctgctg acagcgtggg caatccatgg agcggtgcct
    22321 atatcattgc gagtggcaac ttgttagcag actttagagc taatttaaat gccgaatcac
    22381 aaggaagact tcaagtagca cgtatttacg aaatgatcga tgatcgtggc gtaaaagata
    22441 tgctgtctgt tctccttgca agagatagct atcatcaaaa tctatgggaa tgtgcagtga
    22501 aagaattaga agaaaaagaa ggttcaatct tagtcccaag tactttcccg cgtgacaaag
    22561 aacgaaaaga cattgcttat gacttctata acttctcaga gggagaccaa agtagtgaag
    22621 gttcttgggc tagtggcaaa gcactggatg gcgaaggcga atatcaatgg ttgaaagaac
    22681 ctaagatcga aggcgctgca ccaaaattag aaccagtaac accaaaaatg tatgggacac
    22741 ctccattcaa gaaataaata tctacccgta gaaacacccg ttttttgttc cacacgcttt
    22801 gtgagcaaaa ctgactaaag tcattaatca taaccaccct tcaaaagggt ggttttctct
    22861 agagctataa gctcaaatca ccggccagcg cctaaagacg ctggctttca tattttatgt
    22921 tcaagccata ttgcctttgc cagttttgcc cacctcttaa aaggtttatg tactactcct
    22981 tagcccttaa aaggatcgtc ttactctcgt acactcagct tatctaacgc actatcatgt
    23041 ttttcttgtt cttgaatgta ctttttgatg gtcgcttcat tttgtcctac ttttgtcaca
    23101 tagtatcctt ccacccaaaa gtgtcgatac ccaaatttat attttagatt tgcacaatct
    23161 cttgatgatc tcctgaatat tggctcgata ttgattataa attatttttc ttctatactt
    23221 tggtgtgaat acaatgtggt atttacacat ctactttgta cgggctaaac tatttccctt
    23281 tattgctcca cccgcatagc aagtggtttt gtatttcaat cgctcggcgc gctcaattag
    23341 gtctaaagac aataaatcta aaaacccgct gcttcactga cagaaacaac gggttttcag
    23401 aaaaaaatga aatgaaaaag atctcttacg gcaatctatt ttattttttt gattttttat
    23461 aaatggaaaa tgcttcaaga agtccaagaa ataaagccgt ataaaaacaa gaacctatga
    23521 aatctttatt tattagatat tcaacagaaa tcccgataac actagccaac aatatattta
    23581 tcagaagtgt aaaataagga tgctttttat agaatagaac tagtgttgtt ttcatagact
    23641 ctcctttctt catatttcca attaatcaca aattcacggc attactagaa aaattaaagg
    23701 ggcgcagaag tggaaaaata ctcttcgact ttccctacaa accaggccat cttgtgttta
    23761 ataactatac caaatctaag gtacctctaa atctaaattc catttgtggc ggtgacctcc
    23821 aatttttaac tcttctatct cccatccagt atacataacc ttgatacttc gaccacttat
    23881 atatcgaaca cacaaatacc tatccattgc aggagtacta cctaacttag ttgctacttg
    23941 catctaaata acagctgaaa agataggact tactcttagt aaagtatctg aactattaat
    24001 ttttaaacta tcatttttat cctcaactac atgcacatct gttgcaactt cactttctgt
    24061 ctcatccaga taaatgatta aggaatcggg aattgcaata tcttcagctg acgctggaat
    24121 agttgagcta aattagtcat catatcttta ttataaaata ac
//
LOCUS       DASJUF010000037        23830 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJUF010000037 DASJUF010000000
VERSION     DASJUF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.037"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..790)
                     /locus_tag="U4B62_001318"
     CDS             complement(56..790)
                     /locus_tag="U4B62_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2141486.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="U4B62_001319"
     CDS             1117..1527
                     /locus_tag="U4B62_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN2141487.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="U4B62_001320"
     CDS             1520..2221
                     /locus_tag="U4B62_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN2141488.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="U4B62_001321"
     CDS             2221..3807
                     /locus_tag="U4B62_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141489.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="U4B62_001322"
     CDS             3826..4047
                     /locus_tag="U4B62_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN2141490.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="U4B62_001323"
     CDS             4044..4805
                     /locus_tag="U4B62_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN2141491.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="U4B62_001324"
     CDS             5185..5694
                     /locus_tag="U4B62_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2141492.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="U4B62_001325"
     CDS             complement(5769..6308)
                     /locus_tag="U4B62_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN2141493.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="U4B62_001326"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="U4B62_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN2141494.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="U4B62_001327"
     CDS             7321..8172
                     /locus_tag="U4B62_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN2141495.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="U4B62_001328"
     CDS             8200..8835
                     /locus_tag="U4B62_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN2141496.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="U4B62_001329"
     CDS             8855..9628
                     /locus_tag="U4B62_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2141497.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="U4B62_001330"
     CDS             10036..10833
                     /locus_tag="U4B62_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN2141498.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="U4B62_001331"
     CDS             10811..11224
                     /locus_tag="U4B62_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2141499.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="U4B62_001332"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="U4B62_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2141500.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="U4B62_001333"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="U4B62_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN2141501.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /gene="maf"
                     /locus_tag="U4B62_001334"
     CDS             16001..16561
                     /gene="maf"
                     /locus_tag="U4B62_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN2141502.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="U4B62_001335"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="U4B62_001335"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN2141503.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="U4B62_001336"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="U4B62_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN2141504.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="U4B62_001337"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="U4B62_001337"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN2141505.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="U4B62_001338"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="U4B62_001338"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN2141506.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="U4B62_001339"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="U4B62_001339"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN2141507.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="U4B62_001340"
     CDS             21908..22486
                     /locus_tag="U4B62_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2141508.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="U4B62_001341"
     CDS             22538..23020
                     /locus_tag="U4B62_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN2141509.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="U4B62_001342"
     CDS             23137..23511
                     /locus_tag="U4B62_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141510.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7784 a         4286 c         5116 g         6644 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttatttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt
//
LOCUS       DASJUF010000038        23677 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJUF010000038 DASJUF010000000
VERSION     DASJUF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.038"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..734
                     /locus_tag="U4B62_001343"
     CDS             33..734
                     /locus_tag="U4B62_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2141511.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1343..2359)
                     /gene="tsaD"
                     /locus_tag="U4B62_001344"
     CDS             complement(1343..2359)
                     /gene="tsaD"
                     /locus_tag="U4B62_001344"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN2141512.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2356..2820)
                     /gene="rimI"
                     /locus_tag="U4B62_001345"
     CDS             complement(2356..2820)
                     /gene="rimI"
                     /locus_tag="U4B62_001345"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2141513.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2827..3369)
                     /gene="rimI"
                     /locus_tag="U4B62_001346"
     CDS             complement(2827..3369)
                     /gene="rimI"
                     /locus_tag="U4B62_001346"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2141514.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3353..4075)
                     /gene="tsaB"
                     /locus_tag="U4B62_001347"
     CDS             complement(3353..4075)
                     /gene="tsaB"
                     /locus_tag="U4B62_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN2141515.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4266..5246)
                     /locus_tag="U4B62_001348"
     CDS             complement(4266..5246)
                     /locus_tag="U4B62_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2141516.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5270..6754)
                     /gene="galT"
                     /locus_tag="U4B62_001349"
     CDS             complement(5270..6754)
                     /gene="galT"
                     /locus_tag="U4B62_001349"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN2141517.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6766..7755)
                     /gene="galE"
                     /locus_tag="U4B62_001350"
     CDS             complement(6766..7755)
                     /gene="galE"
                     /locus_tag="U4B62_001350"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN2141518.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8003..8170
                     /locus_tag="U4B62_001351"
     CDS             8003..8170
                     /locus_tag="U4B62_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN2141519.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8232..10043)
                     /locus_tag="U4B62_001352"
     CDS             complement(8232..10043)
                     /locus_tag="U4B62_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN2141520.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10040..10405)
                     /locus_tag="U4B62_001353"
     CDS             complement(10040..10405)
                     /locus_tag="U4B62_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN2141521.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10568..10963)
                     /locus_tag="U4B62_001354"
     CDS             complement(10568..10963)
                     /locus_tag="U4B62_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2141522.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10981..11943)
                     /locus_tag="U4B62_001355"
     CDS             complement(10981..11943)
                     /locus_tag="U4B62_001355"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN2141523.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11943..12155)
                     /locus_tag="U4B62_001356"
     CDS             complement(11943..12155)
                     /locus_tag="U4B62_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN2141524.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12176..12874)
                     /locus_tag="U4B62_001357"
     CDS             complement(12176..12874)
                     /locus_tag="U4B62_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN2141525.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12894..13436)
                     /locus_tag="U4B62_001358"
     CDS             complement(12894..13436)
                     /locus_tag="U4B62_001358"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN2141526.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13568..14572)
                     /locus_tag="U4B62_001359"
     CDS             complement(13568..14572)
                     /locus_tag="U4B62_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2141527.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14569..15558)
                     /locus_tag="U4B62_001360"
     CDS             complement(14569..15558)
                     /locus_tag="U4B62_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2141528.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15555..16361)
                     /locus_tag="U4B62_001361"
     CDS             complement(15555..16361)
                     /locus_tag="U4B62_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141529.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16527..17483
                     /locus_tag="U4B62_001362"
     CDS             16527..17483
                     /locus_tag="U4B62_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2141530.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17560..18078)
                     /locus_tag="U4B62_001363"
     CDS             complement(17560..18078)
                     /locus_tag="U4B62_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2141531.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18166..18315)
                     /gene="rpmG"
                     /locus_tag="U4B62_001364"
     CDS             complement(18166..18315)
                     /gene="rpmG"
                     /locus_tag="U4B62_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2141532.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18543..18989
                     /locus_tag="U4B62_001365"
     CDS             18543..18989
                     /locus_tag="U4B62_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2141533.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19184..21079)
                     /gene="asnB"
                     /locus_tag="U4B62_001366"
     CDS             complement(19184..21079)
                     /gene="asnB"
                     /locus_tag="U4B62_001366"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN2141534.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(21404..22378)
                     /gene="bsh"
                     /locus_tag="U4B62_001367"
     CDS             complement(21404..22378)
                     /gene="bsh"
                     /locus_tag="U4B62_001367"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN2141535.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(22712..22799)
                     /locus_tag="U4B62_001368"
     tRNA            complement(22712..22799)
                     /locus_tag="U4B62_001368"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22765..22767),aa:Ser,seq:cga)
     gene            complement(22944..23510)
                     /locus_tag="U4B62_001369"
     CDS             complement(22944..23510)
                     /locus_tag="U4B62_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2141536.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         7132 a         4991 c         4036 g         7518 t
ORIGIN      
        1 aatatagtga acaataagga gaggactttt taatgcgtgt taataaaatc gattttcact
       61 tttcaaaact cagatatcaa cctgattttg aacatttctc ggatgaagag ttccaacaga
      121 tcaaggaaca tactgttctt cgttcttata aaaaaggaca gatccttttc gacgatggtg
      181 atcccagaca acggttgtat atcttgacca aaggactcgt tcgtttagaa cgttatgatg
      241 aaagtgcaac ttattactac tatgactatg tgaatacaaa taaccttttt cctatgtcag
      301 gaatgtttgg agaagagacc tacacttata cagctcaagc gatgactgat attgaaacat
      361 tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct
      421 atatgataca aaaaatttct aaagtagttg aaatacatga attgcgtatc caaatcggat
      481 tgacctctag cgcctttgac cgggtaaaac aaaatttatt cattttgaaa gaagaattgg
      541 gcgtaccaac aaaagatggt catatcacca ttccttatcc gatcacctta aaagaattag
      601 cagtgaatag cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt
      661 ccgggctgtt agattatgac aaaaagcgtt ttactttctg taagacagct actactccag
      721 ctatgagaga ataacgcgaa caaaaataag cagtgaccct gataaatatc agggtcactg
      781 cttattttcc tccctaaaac ttctgaaaga tccggcagtt ttagatcact ggacaagact
      841 caaagatttt ctatgtgtgt ttcagttatg taatattcac tgtttgcaaa aaatactgtt
      901 agattaacta gatgaagcgt cttgtcctca tccgatctag attattcgat attcctcgac
      961 aataaacgat tactcagtga gggcaatcag aggaacggat aatgataaat aagctgtaaa
     1021 aaatagcaaa ataacaataa tgattaaaag tatgtaaaaa atagggcttt gacaaaaacg
     1081 tgtcacagcc ctatttcgat taaacggtgt gtacctgtca actcttcggt cacaagtcat
     1141 aatgttcata aacatgagga gctgtttttt gaaattgttc cttgtgaatc agagttaaac
     1201 gacaggctta cgaactcttg attaaacggc gttcaaaagc ttgcatacgc ggtaataaga
     1261 acagccaggt caaaaacatg agaagccgtt tttcctgggc tgttcttatt acttgatgca
     1321 gggcagcttt ttcacaacct ctttattcag ataaaaccaa gcttggatca gcattcaact
     1381 gatagctagc aaaatttctt ttttgcattt ccacatgtgc tgctgctcca atcattgcag
     1441 cattatctcc acaaagtcga agagggggaa tcactagttc tacctcaggg agtttagttg
     1501 ccaaagcatc ctgcaatcct tcacgcaacc cttgattggc cgctacacct ccagctataa
     1561 tcagttgctt gactggataa ttttgacaag ctcgaagtgt tttagtaatc aatacatcga
     1621 tcacacttgc ttgaaagcta gctgctaaat catttttgtc tagatcttct ccacgctgtt
     1681 gtgcattatg caccaaattg ataaatgcac tttttaatcc gctaaaactg aaatcataat
     1741 tatcttcatg gatcatcgct cgcggaaaat ggtaattatc ttttccctga tgagctagct
     1801 gatcgatttc ttttccgctt gggtaagaaa gacctaatac tcttccaact ttgtcataag
     1861 cttctcctgc tgcgtcatct cttgtctccc caatgatttc atatgatcca tcttcttgca
     1921 tatagactaa ttctgtatgt cctccgctaa caagaagtgc catcaatgga aactggaatg
     1981 gtttaaccag acgggctgca tagatatgcc ctgccatatg gtttacagga ataagcggca
     2041 attggtgtgc ccaagcaaaa gccttagctg cagaaatacc aatcagcaac gagccgacaa
     2101 gtcctggtcc ataagtgaca gcgactgccg ataaatcttc tgccgacaca ccagcttcta
     2161 ccagtgcatc ttccaagcat aaggtgattt gttctacgtg atggcggcta gctacttcag
     2221 gtactacgcc accaaaacgt ttatgactgt ttatttgaga agcaacgatg ttagataaga
     2281 tctctgttcc atttctaacg accgctacac tcgtttcatc acaacttgat tcaatcgcca
     2341 gaatcaattc ttcattcatt tcatcgtctc cgtttttaac tttctgctca taatgatcgc
     2401 atcttccgtc ggattgcgat aatatgattt ccgtttacct aatatacgaa acttttctga
     2461 ttcataaagt tttcttgctg cttcattaga tagacgtact tctaaaaaaa catgcagcga
     2521 atcttttatt tgttccgtaa tcagtaattg taacaaatgc cgtgcatgcc cttttccttg
     2581 ttctgaaact gcaacagcaa tatttgtgat ctcgacttca tctattactt tcgagtatcc
     2641 aataaagcca cagatctggt tatcttcagc taaaagcaga taatcagagt gaggctgttg
     2701 gatgtctgcc aaaaactgca ttttcgtcca aggtgaacca tgtggataag cattttcgct
     2761 aatcatccat acgcattctg ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa
     2821 ctaatctcac tcattttcta aaagttttat catatccaac gcatcttctt ttgtttcagt
     2881 gtaatatctt tttttgatcg ttgttgcttg aaaccccaat ttccgataaa gtctttgtgc
     2941 atcttgatta ctcatacgca cttctaatga catcgtctca cagcgattca ttatagcaaa
     3001 gttctcgatt tcgtcaataa gcagcgaacc gattcgttta cgctgatatg ccggtcgaac
     3061 ggcaatattc gtgatatggc aatctaaacc gaacgctctt gaaccgataa aaccaatcat
     3121 tcttccatta tcaaagatac ctagatatag atgaggaagc ggtgacttca attctgatat
     3181 aaaggcactt tttgtccatg gcaattcgcc attatagact tctcgttcga catccagcag
     3241 ctctttgata tcttctgtag tcaattctct tagtacgtaa cgacgatcga tttgttcgac
     3301 tgttttttct gtataaatca ttttatcttg aaaaaagctt ctggcatacg ctctaaattt
     3361 tttcaacata actttcgtct ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta
     3421 ctcgttttaa atagcgagga agaaaagctt gtttctctgc aactggttca actaaagcac
     3481 ctaattgagc cacagttaca ccacttggaa tatcccatgc tggaactgta ttaatcatcg
     3541 cactaggcaa tatttccttt atttcagaga agaatttgtg acaatcactc ccgacgaaat
     3601 aaatagcttc attcagcttt tgtagttgtg tgaacaaatc agaagcagaa atatgtacat
     3661 cttcaagtac agtttctaat ctattttctt tccagcggta tgccccagcg tatacatttt
     3721 ttcttcgagc atcaaacatg ggcacaataa tattatccac accgatgcaa ttagccgcga
     3781 tggttttcag actagatact cctactagct ctttatctaa tgtataagca atcgtttttg
     3841 ctgtcgttac acctatacga agccctgtat aagaaccggg accatcagaa accgcgattc
     3901 ggtcaatgtc ttttggactt atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca
     3961 gtgttacact gtggttgcgt ttgatatttg tttgtatttg tccaagaatt tgttcgtctt
     4021 ctaccacacc gatcgccaaa gtttggtttg cagtatctat tcctaatgta atcatatatt
     4081 tgatcctttc tgctatgaca ttctcgcctt ccattgtagc atactttttt attctgagga
     4141 tgaaaatacc aaaggcaaaa ctagtacaat aatacatgat tatctgctgg cgggcaaatt
     4201 ttgccccttc ctgaaacaca aaaaagctgc ggtatcactc cattttccca cagcttttcc
     4261 atcttttatt tcgttgattc tcgaataatc aactctgttt ccacaataat ctttcttgaa
     4321 accggtgctt tggaattact taattgaaga atcgtctcca ctgctagctc tcccatccat
     4381 tcggtataaa cttttacagt agaaagcgag ggcgttacat atttcgctac actgacatca
     4441 ttgaagccca tcacagaaat atcttctggt accactaaac ctttttcttg aatcgctttc
     4501 agtgcgccaa tcgctaatgc atcattagct gcaaaaagcg catcaggaac atgcggattc
     4561 gtttttaaat actgcttcac tgcttgatat cctgactcga cagaaaaatc tgcttcgata
     4621 attggacctt cgtcgtagcc tttttctgcc aaaacttgtt gaaatgccat aattctggga
     4681 tcttttagcc gctgatggtt tttcttcgta tattctgttc cagcaagcat agcgatatgt
     4741 tgcgctttat gggagacaaa gtgttcaatg actttttgaa cacttgactt aaagtcgaca
     4801 actagagagt cgattccttg agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg
     4861 gatcctttca tttctaataa ctgagcttcg tcgaattttc ctagtgcgat cgttccatct
     4921 gccggaagat cactaatgga atcccacgtt tctttcaata aagtaacgtt tgcttcttct
     4981 gccttttttt cgattcctag gcgaatagat aaataataaa gatcttcaag ctcttccgta
     5041 tcgtcgtacc actggatcag gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt
     5101 ttcgtataat ttaagcgttc tgctgcttca aaaatttttt gctttgtggc taagccaacg
     5161 gaaagatcag gatcatagtt gagaactctt gaaacagtag caggagaaac tcctgccaac
     5221 tgcgcaatgt ctttaattgt tgccatttgt ctctcctcct tttttacgtt tataaaatcg
     5281 cagtaaatcg ttcaaacgct gcctgtcctt tttcatctcg cttgaatact ccagcgtctt
     5341 ccagtacacg tgcaaaaacc tgacctacag atttttgtac gacatcggtg accgtatctt
     5401 tcgtcaaatc aggatgttct ttttttaatt gttctgccca cggacgatga tattcagcca
     5461 tctggttttc ttctcctaat aagaaatgct ctacttctac tagttctggt ttcaaacgag
     5521 gtggtaaaac agcaagcccc attacttcga tcaacccaat gttctccttt ttgatatgtt
     5581 ggacatcttt atgtggatga aaaatcccat caggatgttc ttctgaaaca ttgttgtctc
     5641 gaaggacaag gtccaactca aagtaattgt cccttcttcg tgcaattggc gtaatcgtat
     5701 ggtgtggtgt accatctgct gaaaaagctc tgatagatac tgtttcatcc gaatatgctt
     5761 tccatttttc caatactgag ttagctgctt caataagcaa gtttttctct gttgattgca
     5821 aacggatcac agacatcggc catttcacga ctcctgcgat gacttgcgga taatcttcta
     5881 gtttgataag tttttctatc ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat
     5941 aatgatcatg agaaagaatc gatcctccaa caattggtaa gtcagcattc gaaccaacaa
     6001 agtaatgagg caagacttca gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt
     6061 tcatcgggcg gtgttgttct gataaaatga tcgaatgttc attgtaataa gcataagggg
     6121 aatattggaa tccccagctt tcaccgtcta gattcattcg aataatccgg tgattggtgc
     6181 gggctggata gttcaagcga cctctatatc cttcgttttc catgcaaagc atgcaggtag
     6241 gatagtctac ttttaccgcc tcgcgttctg ctgcgatttg ttttggatct ttttctggct
     6301 tagataaatt gattgtgatt tccagttcac catactccga ctctgccgga aatttgatgt
     6361 tttttgcgat ggctcttgtt ttgatgtagt catttttctt acttaaatta aagaaatact
     6421 cagttgcttc ctctgggctt ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag
     6481 gtggcggtgt gagaaaatcc attaattgcg cttcaaaaat ctcttgttct gctggcaatt
     6541 ctgaaagaac accattttct cttgccttag cgacaagctg atccaataag tctacagatt
     6601 cttccttaac tggtcttact tcaatgcttt ctagagcatc ttctcctacc atccctaaaa
     6661 gacgattctg taaatataag cggtccattt ccatccatcc gccagcttcg atcgcaagtg
     6721 tggcaaaatc acaaatcgtt tgactgattg acatatctca ttcccctatt cttcataacc
     6781 atttggatgg ctgacatgcc aatcccatgc tgttttgata atcgctttga tatctgtgta
     6841 ttcaggtttc cagcccagaa tctctctcgc tttttcactt gaagcgacta atcggctagg
     6901 atctccagct cttcttggtg ctatttctgc tggaatttct tttcctgtca cttctctagc
     6961 tgcttctagc atttccttga ctgagtatcc tttgctgctg cctaaattga agaagttgct
     7021 ttcatttcct tctttcaagt attccaaagc taaaatatgt gcagcaatca aatcttctac
     7081 ttgaacataa tctctgatac atgttccatc tggtgtatca taatcgtctc cataaacagc
     7141 caaggctttt ctttgaccaa gagctacttg taaaatgatt gggacaagat gcgtttctgg
     7201 tgtatgatct tctccgatag atgcatctgc ttttgctccg gctacattga agtagcgcaa
     7261 agcgacatag cgcatgccat atgcttggtc acaccacttc atcatttttt ccatcatcaa
     7321 tttactttca ccatacggat ttttaggatt agctggtgta tcttctgtga ttggagactc
     7381 tttcggctct ccataagttg ctgcagtaga agagaatacg attttattca cgttgaactc
     7441 gtgcatgact tcaagtaaga tttgcatacc gtatacgttg ttgttgaaat acatcaatgg
     7501 tttttcgaca gattctccta ctaaagagct tgcagcaaaa tggattactc cttcaatcgg
     7561 ttccttttca aatacggaac gtagaaattc tttatcccgg atgtctcctt cataaaaatg
     7621 agcatctgga tgtactgctt gtttgtgacc tgtgagcaaa ttatctacca cagctacttg
     7681 ataccccttt tgaactagct ggtctacagc gtgcgaaccg atataaccgg ctccgcctag
     7741 tactaaaatt gtcataaaaa acactccttt gattagtaaa attgtgtttg ttgattctat
     7801 ataatttaaa aaacgaagtt tacttttatg gtgaaaacaa acaaagtaaa atttcgtaaa
     7861 ttcatatggt aaatttataa taaaacattt agtaaacatt ttcaaggatt ttttgcaaag
     7921 gtttacaaac aaacgtaaaa ctagcctttt tttctctttt gttccataat agatataaca
     7981 aataataaat gaggtggatt caatgaaaaa atttgtatct ggaatattag taggaagtct
     8041 ggcaacagcc gctgccgttg ctggtttggt tgcttctgtg aaaaaaacag tgatcgatcc
     8101 aattgacgaa aaagaagcaa tgatcgaaga aaaccgtaaa aaagcaatgc gtaaacgcgt
     8161 ttctcgttaa taattattta aaaatacgca tgaagacttt ctaaaaaaca gataagtcat
     8221 catgcgtatt tttatttgtc agtcaatata agttgtgcta ttttatctgc ctgtctcaga
     8281 ttccatggat ctgtataagg tctagctact gctgcagtta gccaatccac accttcaaca
     8341 ggtattccat agcaataaaa gtcagactgg atttcccctg aagcatcaat cacgcgattc
     8401 gtacctcgct caacatctat agcttctgaa tgataacttc ctgtattatc tgaaaatgaa
     8461 aaagggcgaa caagtttgtt ttccttcaaa ttttttatga caggattact tgaacgagaa
     8521 aagcttgttt gtggcaatcg agcttcaatc aaatagcgac tgctgatttc tttatcttgt
     8581 agagaaataa catagcggtt gtgcttttgc tgaatatcaa atggcggttc gatcaaatga
     8641 aaaatacccg catctatcag tgctgctaat tctttcgtcc gaatgattgg cggaccgatc
     8701 gttaaaaagc tatttagatg agtaaaccat ccccatagta tttctttgta ttccttagca
     8761 gaaaaaacgt gccattcaat agcctgccgg attggatcac gccagtcttt taatgcatca
     8821 aatgttgaag caaatgctcc tgtgcaattg cctttttccg cttcttttat ctgcccttca
     8881 ataaatattc ggctagcttc tgaaaagggc tcattctgat aggcaagagg agtttctaaa
     8941 tacgaccaat cccatctata agggattagt gctggatgct ttccgagaca ctcctctgaa
     9001 gagagggaga gaaaatcatg ctcaaaattt ttctttggaa tcggcaagtg atattcttcg
     9061 atgattttct tgtaataaaa caactcaacg tcttttttca gatagtcaaa aaatgtttcg
     9121 ccagtcaaaa atcccgactg atgccattgc tccaggtttt catttgttag taaccgtggc
     9181 catgccattg ctccgccttg cttttgattt cttcctcgag ggtaataagg gagccccctt
     9241 cttgatccac tgtatacaat cggttccttg cctgatggat gatagaccag ttttcccttt
     9301 tcgttctcga agcgccctcc tcttgatact gtaaacaatc cgacataatc aaaaaaactt
     9361 aagcctagac ctcttaaaaa gacattttct ttaggcggta tcttcgaaag atctacatct
     9421 gcaggattag aaggaagctg atacagtaaa acatttttct ttgcgtagtc tacccacact
     9481 ctttcttctt ctgtctcatc attttcccag tgaccgcttg ctaaaacaag ctgatcgata
     9541 gaatagcttt gctgatctgt ctgtaaaaga taaccatctt ttcttttgct tacttttttt
     9601 actaaagaat aaacaaactg aatagaagct ggaaattctt cttgcaactt tgagaaaaac
     9661 cagcgttgat agagtccgta taaacatcga gtagattgct gatctttttc cagacgattg
     9721 atttcttcta taattagttg cttatttttg gttaccgtct gttcgatata atctgtagca
     9781 ggtcctttac tccattgaaa caagttgggt ccctgtgtta ccaatcctcc actggacaat
     9841 gtttcatctg taaaaagcgt cacttgttga gaaacagaat tcattaataa ctccggtggc
     9901 tgattcaacc gccaaactcg acctccagga ccattgggat cgaaaagaaa aagttcaaga
     9961 gaatgtctgt cctgattatt atggagaatc ctttctgcta cacttaatcc tctaggtccg
    10021 gcaccaatga tccctatttt catttgcatg cctccatttc gataaatttt ttcaataaaa
    10081 cagcatggtt ctgatgttca ttttttgcac cataaacaaa agtaatgact ggatgttctt
    10141 tcataagttg actgagttta aaaatagctt gcttcttttc ttcttcgaaa atcaattctt
    10201 gctggtattt ctttgcaaat gaagcaaatc gttctggtac atggtcaaaa gttttgcgtg
    10261 cttcattact aggtgcaatt tctttcaacc acagatctaa ctgcgctttt tcttttgaaa
    10321 ttcctcgtgg ccagaatcta tccacgagta ttctatagcc atcctctttt tcagcatcaa
    10381 ggtaaacacg ttttaatttg atcatagttg tttccttctt tctacctttt cgcttagctt
    10441 atatatgtag ataatagcac aaaataaaaa aaatgaacgg cacaactatc cattgcgccg
    10501 ttcatgtgat cttggtcaaa atctaatgat tgattcggtt tttaaagctg tcatacaatc
    10561 atctgtttta tactttttta cttttgattt ttccagacca tccgtcatat ccacccttaa
    10621 gtaaataaat ctcttgataa cctgctttac gtaatttatt tgctgtacgg atgctcaaac
    10681 ttttcttttg atcgtaaata tagacaggct ggtcttttcg aatagatcct aatgaatctt
    10741 tcaaaacagt gtaagggatg tttcttgcac ctaaaatatg cccagcatca aacacatctt
    10801 tttcgcgaac atcgattact tgtgcttttc tcattccttc tcgaaattct tcttctgtca
    10861 aagtcgttgc tgaacgtttg gccattactc gtagatagac ttcccaaagg atgattgcaa
    10921 gcacaatcaa tagcaaaatg atattaatta cccataaaac catgtaatgc ctccgttttt
    10981 ttaagcaaat ttcaaggcta atgaggcagc accaatgaca ccagcttcat taccaagctc
    11041 tgctaatttg atttttgttg attctcttac ttgcgggaaa gtaaattctt taaaatattt
    11101 ctctacacgg ctacgtaaaa attctcctgc tgccgatact cctccaccta acacgatact
    11161 tgacggattt aatgtattcc ccaagtttcc gcaagctaaa cctaagtaga agcatacgcg
    11221 atcaacaacc atcaatgcaa acggatcgtc ttcttgagcc atttcaaaga tatctttact
    11281 tgttacttct tctccgttgt ctagcattgc tttcagtttt gaatcccctg cgtattcttc
    11341 tgccaagaaa cgacctaggc gtaccacacc tgtagcgcta gatacagttt ctaaacatcc
    11401 gcgtttaccg caagtacatt cgaacccttc aggatccaca gtgatatgtc cgatttctcc
    11461 tccagctccg ctcacgccgt gaatcaaatt tccgccagca acgatacctc cgccaacacc
    11521 tgttcctaga gtaacgaata caacgtctgg atcgttttct ccagcacctt tccatcgttc
    11581 acctaaagca gcaacattcg cgtcattatc gatagcgaat ttgattcctg ttcctgcctc
    11641 gatcagtttt ttcacaaatt gaacttctgt ccagtttaag ttatacgcac caataaccgt
    11701 tccagcttcg ctgtccacgc ttcctggggt acccatgccg attccgataa agtcttcggc
    11761 tttcatatta tataaattca aacgatgatt gattgattca atgatttcag gaacgatatg
    11821 cttaccatca tccaaaatat tggtatcgat gctccatttt tgctgaatct ctccttctgg
    11881 tgttaaaatt gcaaattttg ctgttgttcc acctaaatca atcccaataa tcttcttgtc
    11941 cattacaaat ttcctttctt tcggctttct tcaattcggt gctctctttt taatatgcgc
    12001 cacgctgtca tgtatgtatc atgatcaatc aaacggctat catacagatt acgcaattcg
    12061 atagtcatta attcaatgtc atagatgcgt gcacccacat agataaagat gccaaatttt
    12121 ttgaacagct gctgtacatc atataagctc tccatcttta agcacttcct tcatattata
    12181 caagtaaacc atattttttt aaacccagta aaatacatat tacaaaaaga aatacaaaaa
    12241 ctagtgtaga aagaatcctt tggtgtatcg aatagcttgt ctgttgtttc ggtagaccca
    12301 aaacattccc taacactaat ccgccaatta gcccaccgac atgtccccaa acatctacag
    12361 aggtatccag aagaccaaaa acaaatgtca acacaataaa taaaagaaaa tgtctgacta
    12421 gctgctggat cgctgggtta tgtttaaaat gaaaccctaa gataaacaac gcaccgaaca
    12481 aaccgaatat cgaagtactt gcaccaccag acagaacatt cgcttcgtta aaagcaaagc
    12541 ttgctgtatt tcccattatc ccgctgaaca tataaatcaa gaaaaaacgc caatggccat
    12601 agattgcctc gatttgctgc cccataaaat acagtatcag tgagttcatt acgaaatgca
    12661 tcagtccaaa atggatgaag attggtgtca ctaaacgcca ccattcattg aagtgaacaa
    12721 gataaggacc atacattccg gcataataag ggatctcaag tcgcggaaat aattccatca
    12781 tgaggaagac cgctgtctgt atccctaaaa aagcataggt ccataacggt cgtcttaacc
    12841 atacacgcaa ttttatttgc tgttgataat tcatgaaaac ctcccatttc attttagtca
    12901 ataaacaatt gttggatctt ctgatcaaag ctctccggtt gccattgttc attcaattgt
    12961 tcacgaaaaa caaggctaca tgttttttta tgaaaattat ttaaataccg gtcgtaataa
    13021 ccaccgccaa aaccgattcg ataaccggct ctattgtaga taatacctgg tacaatgagg
    13081 agatcgatca tctccggcaa atagagcttc tctgattgtg gctcaagaac accaaaactc
    13141 gaacgtacaa gaacggactg ttcatccatt tcataaaacg ccatctgacg tttcggcaaa
    13201 gttttaggta caactatttt cttttgttct ttttctgctc gttggataac cggctgtgta
    13261 tccagttcaa attcattcga taaagtcgtc cctatcactt ttgctgactt ccagtactcg
    13321 ctggcgaata gctgtttata gattcgctgt tctttttcct ttttcttgcc acttttggcc
    13381 agatttttca gcttttcgat tcctttccgg cgtaatctta ctttcaattg gtccaaaaac
    13441 tcacccttcc ttctctcata aaatggtata ggaaaaaaag aaaaatagca atcgaacttt
    13501 caaataaatg cataaataga ataaaaagaa gacctagaaa tgattggcct tcctttttta
    13561 atgaatttca actgttcagc gcgtgttttt gtctgataaa aagtgcatac agaaaatatg
    13621 gcgcaccaaa aagggcaatc aaaattcctg taggtacttc cccacttagt agaatcactc
    13681 tagcaaccat atctgcacta agcagaaaaa tggaaccgaa caaaccgctc agaacaaagg
    13741 tccagttatt ttctttacgt acgactagct tcgcagcatg aggagctagt agtcctacaa
    13801 aagataaact acctgtcacc gctacactac cgcttgccaa agcaacagct aacaacaaaa
    13861 ggattttttg acttttagcc acgttaaggc ctaatccttg cgcgcgttct tgaccaaatg
    13921 atagcaatac aatcctctgt gcataaagtc ctcctagcgg aattaagata accgcccatg
    13981 gaagcaatgc ccaaacataa ggccagctcg caccccagat tgatcccgct aaccatgcat
    14041 tcacaaatcc ataattttcc ttagataatt ggatcgtgca tagcaatgtt aatgcagaaa
    14101 ggccggcatt aacggcaata ccggacaata agattcgatt catacccaaa aagccatttc
    14161 gatcataggc cgcaagatag acacagcaag cagctaaaac accacctaga aaggctacaa
    14221 ctagcaatag aaacggcagc gaatagtgcg aaaagaaacc taaataaagc atcactaaaa
    14281 atcctgctcc ggcattaatg cccaatatac tagaatctgc caattcattt cttgttactc
    14341 cttgcaagat ataaccagaa acgccaagac aagctcctgc taataaactg atcagtaatc
    14401 gaggaactcg aaattctcta acaatcagcg ccaaagaaga tgattctttc cctatgaacg
    14461 tggcaaaaag ctgatccagg taaataaaag gcgtaccaat cattaaacta cctatagaca
    14521 ggatgacaag tacaaaaagc aagaaataga acgtccaata tatttttttc accgttgttc
    14581 cctccttact tgtatcagta aaaacggaac gccaatcaat gcaatgacta atccaaaagg
    14641 tgtttcaaaa gggggattga tcatcctcgc tgtaaaatcc gccaacataa ccaatccacc
    14701 gccaactaaa aaagagacag gtagaacata acgatagtcc gttccaacca ttccgcgaac
    14761 aactggcgga accaacagcc caataaatga aacaggtccg actaacgaaa cggcaatggc
    14821 tgctaacact gcaactagac agaaaacaat caaccgcgtt cgtttaggat gacttccaag
    14881 agctaatgca tgtgtttcac tcaaatgaat caaagatatt gcttttccta atgaaaaaga
    14941 aaccaaaaca gcaagtatat ataaaggaaa caatactgct aattgtttcc atgtgatatt
    15001 ggcagctccg cctacaaacc aaaaagcaag atcctgctgc agattaaaga attgggtaag
    15061 tgcttggctg atagcagtaa aaaaagaact aatagctacg ccagctaaaa tcagctgtaa
    15121 aggattcatg cccattttcg aaaactgcaa aaaaccaaat acgagcaaag ttacagcaaa
    15181 agaacctgcc aatgacatta gaaacacaga aaaatcagaa gctttaggcc aaaaagcaaa
    15241 actaagggcc attgccaacg aagcacccgc gttgattcct agtaagcctg aatctgctaa
    15301 aggatttctt gtcactcctt gcatcaatgc accgctcaat gcaaaacaac tccctacaag
    15361 aaatgctccc gccacacgag gcaaacgaat atttcgtatg atttggtgac tttgttttga
    15421 tctatcaaaa tcaaacaacg cctgccaaaa atctgctttt cccacagtcg ctgcgccatt
    15481 tgaaagagca acaaaaaaaa taatcaaaag aacaaaacag accaaaagca ggagtctatt
    15541 tttcttcata gtcctcataa ttgatcctcc tgctcttttg gcaacaaatc ataggttaga
    15601 aaaactggtt tatttgttct tggaagcgtg acaatcgttg catcaatctc aaatattttt
    15661 ttcatacact cttttgtgat cacttcttca ggtgtacctt ctgtgatcaa cttaccgttt
    15721 ttcattgcta ataaataatc tgaaaaacgt gaagcatgat taatgtcatg aatcgacatc
    15781 aagatcgttg tttgtttcat ttgattgatt tcagtcaata actgtaggat ctctaattgg
    15841 tgtgccggat ctaaaaatgt tgtcggttca tccaagatca agatatctgt ttcctgagca
    15901 agcgccatag cgatccagac acgctgggtt tgtccaccag acagcgttgc gatatcccgc
    15961 atacgaaatc cagctagacc tgtagcatcc aacgcccact cgattgcttc atgatcttgc
    16021 gtgtccatgc cactgaacag tttttgataa gggtatcgtc cgtaagatac tacttcttct
    16081 actgtcatac ctgtagggtg ttcactagtt tgtgctaata atgcaatttt ttttgcaagt
    16141 tcttttgaac tgtatgtaga gatatcagag ttattgacaa tgatctttcc tgatttcaca
    16201 ggtagtatac gggtaaaact tttcaataaa gtagattttc cacttccatt tggcccaatc
    16261 aaactagtga ttttcccttc aggaatcgta acagacatgt tttcaatgat ttttttgttt
    16321 tcgtagccgg ctgcaatgtc agctgctctt aatctcgtca tttcacccat cctttcgctg
    16381 aaaatcattc tcaattatga cgctgtttca acatttcgtc aagcgctact ttcatatcta
    16441 tagccaaatg aagcggttga cagcgttcga aatatcagga ataataacca atgagaaaga
    16501 ttctcaatta tgttaggagg aaagaaatga aattaaaaac aaaagtattc acattatcta
    16561 gcatattgct gctggctgga gtactgagcg cttgctcccc ctcttcagat aaagaaacag
    16621 ctacaactgc caattcgacg acttcatcca atagtactca tacagtcaca gatacacttg
    16681 gacataaagt agagattcca aacgcgccca aacggatcat cggcagttac ttagaagact
    16741 atctgattgc actaggggaa aaacctatcg cccagtggac tgttggcagt ggttccattc
    16801 aagattatct gcaagatgat ttaaaggatg tcccaacgat ttcttatgac cttccttatg
    16861 agaaagtgtt aagctttgag cctgatttgc tgttgatttc ttcttccgct actgtcgaag
    16921 gcgggaaata cgaacaatat agcaaaatcg cgccaactta tgttgtaaaa aacggaacag
    16981 atgtcacatg ggaagaacaa ctaaaagatg tcggcaaagc tttggcaaaa gaaaaagagg
    17041 cagaaaagat catttccgat tatcaaaaat ccatcaagaa aactcgagaa gaactagcag
    17101 ataaaatcga gaacaagaca gctgcagtgt tgtgggtgac caacaactca gcctttatgg
    17161 tcagcaaaaa tcggtcaagt ggaagaatcg tatacgacga tctggaattt ggtgttcctg
    17221 atctagtgga agaggtctcc aaaaaagcca cttctgactg gtctgctgtt tcctctgaaa
    17281 agctagctga attagatgct gactacatca tccttgtgaa cagtgacaaa gatgctgcca
    17341 tgttcaatga accaagctgg aagaatctta aagcagtgac agacaatcat gtactgcaat
    17401 tcggacctga atctagttgg ctatataacg gacctattgc cagtcaggaa atggtcaatg
    17461 atatcaaaga aaaactaaat tgattttaac taaaaaaagc atcttcacga ttagtcgtga
    17521 tatgcttttt ttcttattaa caaactagac gcatagattt tattcttctt ttgaagcaac
    17581 cctatttttt actctttccc atttttgaaa acgatgggca tatagatttt cttgatccgt
    17641 tatcccctca tactcctcta taagggcgaa gtctgaaaaa tcaatattcc caatatacgt
    17701 atcgccttca aacgtgctat cgatcaatgt tcgccagatc cgaccagtgt aaggaagaag
    17761 ttcttgaaaa atccgactac cacccgaaac atagatatct tctgagatcg tttgactata
    17821 cgacaaaagt tcatcgatcg aatgcattac ttccgcattt tctttcgtgc gaaaatttgg
    17881 ctgcgttgtc aacacgatga tatggcgata aggaagtgaa agatccccca ttccttcata
    17941 ggtctttctt cccatcacta aaattcgatc cattgtatgt tcgcgaaaga aacgcatatc
    18001 atttggcaat cgccatggta acaaaccatt tttgccgatc agaccatttt tatcttgtgc
    18061 ccacatggaa ataaacatat tatccgctcc ttatgatttt agatgttttg agataaaaaa
    18121 agtctcaatt ctttgaccag caaggaattg agaactttaa aaacattatt ttgtttcacg
    18181 gtgcaaagta actttgcgtt cgcgtgggca atatttttgt ttttccaaac gatcaggatt
    18241 gttacgtttg tttttgcttg ttagatagtt acgttcttta caagaagtac actctaaagt
    18301 gatgtttacg cgcatgtttt tccctcccat ttttacaaga atcctctaag atgactctca
    18361 gacttgatta tcttaccatg attttttcta gattgctagt gtttttcacg attttgttaa
    18421 gtaaaactac ttgacgataa atacaacggt tttacttctc aaacaaaatc tttttctttt
    18481 tgtataaata cccttacact tgtttatata gattatgttt tttaataaag ggggatacta
    18541 atatgccatg gaacatgaag gactttcctg catcaatgaa aaacttagat aaattgacaa
    18601 gaaaaaaagc aatcgacatc gcaaatgcat tattagatga agggtaccct gataaccgtg
    18661 cgatcccaat tgccattgat caagcaaaaa aatggtatga agatgctagt gaatcagaaa
    18721 aacgaacttt cgaaaaagaa aaaaatccaa gcaagacgga tgaacacgat acgaatcctc
    18781 gtgctggaaa actgcttgat tcagatgtga tcgtagaata tgaggaagaa caatggatcg
    18841 tgaaatctaa aggggcaaaa aaggcaagca accattttga tacaaaaaaa gaagcaatcg
    18901 aaaaaggaaa gcaagtagca caaaacaaag aatcaacttt gttcatttat aaaaaagatg
    18961 ggacaaaaga aaaagaaatc gattattgaa catatgtaaa ggggttgtga cgaaaatcgt
    19021 gtcacaaccc ccttatgttc gaaacaatgg tgttcctctg ccaactccga accataagtc
    19081 ataatatcta taaaacggga agagctgttt tttgaaattg tttcttatca ctcagtgtta
    19141 aacggtagga gaaaacctct tttttattga tatttgattt tcatcaaaca gtcacatagt
    19201 cgattgcttc tgcttgcgga atcgtttcat ctacaaagta gactttatac catgtcaaga
    19261 aactgaagat tgtccagatt tttctttgct cttctttacg tccttcgtga tggtcatcta
    19321 gcaacttcat gattttttct tgatcaaaaa attcttttgc aaaatctgct tcgaacaatg
    19381 ctttgacttg ttcgtagccg tgttcttctt tcaaccaagc tttgattggc acagggaatc
    19441 ctaatttcac acgattcgac catttttccg gaagatgttt gttcgcagct tgtctgaaaa
    19501 tatcttttgt gttgttttga ttcaataaaa acttacttgg tatttgttga gccaatttca
    19561 tgacttcgat atccaataaa ggtactcgga cttccaaaga acttgccata gaaagtttat
    19621 ctgcctttaa caagatatct tttggcatcc attgatgaag atcgacatat tgcatcttat
    19681 tgacttcatc tttgattcct tctgttttct cgtaatgaac agtcatgatt tcatccacag
    19741 atggtgctgt ttgatatttt ggtgttaaca cttctgatgc ttctgattct tcgaacactc
    19801 gagcatgacc gataaaatat tcttttgcag gagcaagtga ttcatataaa tggatccgtc
    19861 catggaaatt tttcattttt ccgattttac gtcctaaatt atagcgtgtt ccttttggta
    19921 attttttcaa tccttgagca atcacacgga tgaattttga attcgtatgg aatccatacg
    19981 tttggtatcc cgcaaacagt tcatccgccc cttctccaga ttggactacc cgcacacttt
    20041 gtgaagcaag atttgctaaa aagtacaaag gtacgcaaga aggatttgaa tcaggttcat
    20101 ctaagtgata ctggatcaaa gggaatgctt tgaacgacat gtctccgtcg atcactgctg
    20161 ctgtattatc taagtccaat agttcggtta gttttctggc ttcaatcgct tcattatagg
    20221 ttttatcatc aaacccgatt gagaaagaat gatctggttt caatacagag gttacataac
    20281 tggaatcaac cccgctggat aagaaagaac caacttctac atcactgacc gtatgtgctt
    20341 caatagaagc ttgaacggtt tcgtcgattt tttcgatcca ttcttgtcgg gaatgcgttt
    20401 cttttgtttc aaaatctgca tcccaatatt gctgaatgtc tatttttcca tctttatacg
    20461 tataataatg gccttcaggc aaacgataga catctttgaa gaatgtttcg ccgtttaatg
    20521 gagaatattg gaaagtcata taaggtttca acgcttcttt gttcaattct ttgttaaaat
    20581 caggatgagg cagaaaactt ttgatttctg aaccaaacat aaatgttccg ttcatttctg
    20641 cataataata tggtttgatc ccaaagtggt ctcttgcacc gaataactca ttcttttcat
    20701 tatcccaaat agcaaaagca aacattccgc ggatcttttg caataattct gttccccatt
    20761 cttcgtaacc atgcaaaagg acttctgtat ctgcatgtgt ttgaaaaacg tggccagctg
    20821 caatcaattc ttctctcaaa ggctggtaat tatagatttc gccattaaaa ataatgatct
    20881 ttgtccgatc ttcattgtaa ataggctgag taccgccctc taagtcaata atacttaatc
    20941 ttctgaatcc taaagcaaca tgtttatcga tatattctcc tgaactatta ggtccacggt
    21001 gaacgattcg atccatcatt gtattaataa ttgttttttt gttgtccttg tcgttaacaa
    21061 aacctacaat accgcacatt taactttcac atcctaatat aaaattactg tttatttcat
    21121 tcatatgcat cttatcatag aataaatagc ggacgaatat tttctctctt acaatttcgt
    21181 cacgtgaaaa caaaagatga aagtttctgt ttttacagaa aataaagtcc caatatctaa
    21241 tttaccatgt tttctttttt gaggctacct tattctcttc gtgttaaaaa aatattagtg
    21301 gatgctattt ccaaaaaaac cagcaaaaaa agacacgacc taaagatcgt gtctaattat
    21361 gactgtatca gaaaatatga taccaaacgg atctattcat taattaattg acatagttta
    21421 tctgttgctc ttttatgact ggataactga ccaattcatg acgatccaag tcttctttat
    21481 tcatatttac agcagtaatt tgactgtctt catacgtacg ataataataa atccctttat
    21541 caacgttaca gcaagaagaa taaatcgtat attcgtattt tctttctcca gtatcgcata
    21601 ctcctttttg ttgctcgact gagcctagta tatgaaaaaa ttgactgata ctttcagatt
    21661 ctgaatcacc tgatgcagca ttcattttag taaatgtggc ttttacaaat cgagaaacag
    21721 aagagagatc tccaggtaag cccaatcctc ccatcccacg actataaaca ttcaagctga
    21781 tctggtttga aaaatgattt tctggtgttc catttgatag cgagcgataa ttgttcagat
    21841 taaataattg ataatcaaat ggcggattat ttgtcaacac accgacagga ttatcatata
    21901 aatgaagtcc atctttcata ctttcaatta ctatcgattt ttctttatca gctaataacc
    21961 agtgtaaagg tgacaagggt agtttttcac taaaatcgat attgactaga ttgatagtag
    22021 ccaataattc tttagcttcg tctatggttg agcattgccc tagtatccat ggaataaact
    22081 caaatggtgc aacgttatct tttccttctt gtaattcttt ataatcagca ttgccaggaa
    22141 aattcagtcc tgccatactc aacccttttt catttgtagc ctcataatag agtggatagt
    22201 tggatacccc agctgctatt cctatcattg cataatgttt atctaaattt tttacctttc
    22261 taaaatcgaa acgataattt cttggagtaa tagtgaccac ttcattataa gacatctcat
    22321 aatcaaaatt ccttccaaaa taatggtctt tcgttgcata agtgattgcg gtacacatcg
    22381 tttcccctcc taaaacaaat cgtttttaca cttttatctt acatctagta ttttttttat
    22441 caaaaaaagg ccaaagaaga ttgatttact agcatttata aaaaatagta ctcaggggga
    22501 atcaaaaaat attgtaggtt aaaagtacaa caataagggg ctgtgacaaa agtcttgtca
    22561 cagcccctta ttccgaacaa acggtgttca aaagcttgca tctgcggtaa taagcccaat
    22621 caaacgaaaa aataggaaaa gctatttttg tttggccgtt cttattactt gatgcaggac
    22681 agcttcttca caacctctaa tctttgatat aaggaaggta caggatttga acctgcgcgc
    22741 cggtattagc cggttcgccg gatttcgagt ccggtgcatt accactctgc caaccttccg
    22801 taagtactat ttttaaactt ttttcacgaa atgtcaagcc tatttctaat agataaataa
    22861 cagaaatcgc tttttttagc aaaaaagtcc attgacagtt tgcagccaac ggacttaccg
    22921 atcaaattca tactgataga acactatctg attatttcag gattatctaa tgctaaaatc
    22981 gagtcaattg tccgtttgat cagtgcaatg atttgttctt ctgttggatg catgatgttt
    23041 gatgacatca atgacgctaa gccagtcact gctacccatg taccagtatg cagagcatcg
    23101 acatattcat ccgataattg attgtatctt tcatcttctt taactatttc acggaaataa
    23161 ttgtatgaaa aatcatgcat tttctttccg tccccatgtt catcaatgta caatgccatg
    23221 taaagactat gatgcttttt tgcaaagtag atatagttca aaccgagatc aatgatttta
    23281 tcaccagtac gttcaatagg gaaaacatct tcttttagtt gttcaaagat agaatccagc
    23341 aacgtatttt ttaaatcttc catgtttttg aattccagat aaatcggctg tgtcgaaatc
    23401 cccatctttt tagcaatatt tctcgcagta aatcctttaa acccttcctt tgcaacaaca
    23461 tcataagcag cttttagaat ttgatccttg gtatagactt ttcttctcat taaaactccc
    23521 ccacttcttt actgcagtat atgttgtttt attgtaccct atttttagca ttttttgcaa
    23581 acattttact caaaacgtta tattttaaat tttttttcag tcttctttaa cttttttttg
    23641 caaaattaaa aaaatttggt taaattcaat gggttcc
//
LOCUS       DASJUF010000039        23518 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJUF010000039 DASJUF010000000
VERSION     DASJUF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.039"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(209..916)
                     /gene="deoD"
                     /locus_tag="U4B62_001370"
     CDS             complement(209..916)
                     /gene="deoD"
                     /locus_tag="U4B62_001370"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2141537.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(933..1751)
                     /locus_tag="U4B62_001371"
     CDS             complement(933..1751)
                     /locus_tag="U4B62_001371"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2141538.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1775..2941)
                     /gene="deoB"
                     /locus_tag="U4B62_001372"
     CDS             complement(1775..2941)
                     /gene="deoB"
                     /locus_tag="U4B62_001372"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN2141539.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3040..3996)
                     /locus_tag="U4B62_001373"
     CDS             complement(3040..3996)
                     /locus_tag="U4B62_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141540.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3996..5129)
                     /locus_tag="U4B62_001374"
     CDS             complement(3996..5129)
                     /locus_tag="U4B62_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141541.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5122..6684)
                     /locus_tag="U4B62_001375"
     CDS             complement(5122..6684)
                     /locus_tag="U4B62_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141542.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6896..7978)
                     /locus_tag="U4B62_001376"
     CDS             complement(6896..7978)
                     /locus_tag="U4B62_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2141543.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8044..8442)
                     /locus_tag="U4B62_001377"
     CDS             complement(8044..8442)
                     /locus_tag="U4B62_001377"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN2141544.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8463..9125)
                     /gene="deoC"
                     /locus_tag="U4B62_001378"
     CDS             complement(8463..9125)
                     /gene="deoC"
                     /locus_tag="U4B62_001378"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN2141545.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9171..10472)
                     /locus_tag="U4B62_001379"
     CDS             complement(9171..10472)
                     /locus_tag="U4B62_001379"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN2141546.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10613..11629)
                     /locus_tag="U4B62_001380"
     CDS             complement(10613..11629)
                     /locus_tag="U4B62_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2141547.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11937..12542)
                     /locus_tag="U4B62_001381"
     CDS             complement(11937..12542)
                     /locus_tag="U4B62_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2141548.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12738..13661
                     /locus_tag="U4B62_001382"
     CDS             12738..13661
                     /locus_tag="U4B62_001382"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN2141549.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13720..15285
                     /gene="guaA"
                     /locus_tag="U4B62_001383"
     CDS             13720..15285
                     /gene="guaA"
                     /locus_tag="U4B62_001383"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN2141550.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15776..17407)
                     /locus_tag="U4B62_001384"
     CDS             complement(15776..17407)
                     /locus_tag="U4B62_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN2141551.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17421..19094)
                     /locus_tag="U4B62_001385"
     CDS             complement(17421..19094)
                     /locus_tag="U4B62_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN2141552.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19107..19424)
                     /locus_tag="U4B62_001386"
     CDS             complement(19107..19424)
                     /locus_tag="U4B62_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2141553.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19449..20849)
                     /gene="lacG"
                     /locus_tag="U4B62_001387"
     CDS             complement(19449..20849)
                     /gene="lacG"
                     /locus_tag="U4B62_001387"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN2141554.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21086..21871
                     /locus_tag="U4B62_001388"
     CDS             21086..21871
                     /locus_tag="U4B62_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2141555.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21874..22566
                     /locus_tag="U4B62_001389"
     CDS             21874..22566
                     /locus_tag="U4B62_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2141556.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22840..23211)
                     /locus_tag="U4B62_001390"
     CDS             complement(22840..23211)
                     /locus_tag="U4B62_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN2141557.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         6790 a         5246 c         4233 g         7249 t
ORIGIN      
        1 agtcacaata ttcatcaaac atgaaaagct gttttttgaa attgttcctt gtgactcaga
       61 gttaaacggc agattcacaa cctcttggta aacggtgttc aaaagcttac atttgcaata
      121 ataagtccag ccaagtcaaa aacatgagga gctatttttg cttggatgtt cttattactt
      181 aatgcagggc agctttttca tttccttctt attctgctat cgctgtttcc aatcctactt
      241 cgatcatttc attgaacgta gattgacgtt ctgcagctgt tgtttcttca ccggtgatca
      301 aatggtcgct aacggtcatc aaacttaatg tctgcacatg gtatttcgcg cctaagtaat
      361 aaagagccgc tgcttccatt tccactccta aaacgccaag ttcagctaat tggaacgtct
      421 ctgtcaaatc atctttgtag aaagaatctt ccgaaagaac atttcctaca tgaaccgtat
      481 agcccttttc tttagctatc tcatatgctt tcagtaatag attgaaatca ctgattggag
      541 gaaaatgaaa ggcttggaaa tttttttcga tcatcgaaga actggtcgct gcaccttgtg
      601 ctagtactaa atcacgaaca tggacgtctt ttgacaatgc accgcatgta cctactcgaa
      661 tcaatttctt tacaccatac gactggatca attcatgggc atagatggtg gctgatggca
      721 ttcccattcc tgttccttga acagaaatac gttctccttt atatttgccg gtaaagccta
      781 acataccgcg tacctgattg taacaaactg gatcttccaa aaaagtctcc gcaatatatt
      841 tagctcgcaa aggatctccc ggaagtaaaa tcttatcggc gatctcgcct tctttcgctt
      901 caatatgcac actcatttta ttgttcctcc tattacaata atgtcaatgt ttctttaacc
      961 aatgctttaa aagattgttt gactctctga gttgtttcta caacttcttc atggctcaga
     1021 ttcgcttgca tacctgctgc taagttggta atacaagaga ttcctaacac ttttaatccg
     1081 ctgtgagctg ctacgatgac ttctggaaca gtcgacatcc ctactgcatc ggctcctatt
     1141 gtccgagaca ttcggatctc cgcaggcgtt tcataagttg gtccagaata gcccatatat
     1201 acgccttctt taagatccaa acctagtttt tctgctgctg cttttgctgt tgcttgatag
     1261 tcaggcgtat aagcttgact catatctgga aatctagggc ccattgcatc ttcattcgga
     1321 ccgatcaaag gattttcacc agtaaaattg atatgatcgg taatcagcat caaatctcct
     1381 ggtacaaatg attcgttgac accaccacat gcattagtca caatgactga atgggcccct
     1441 agtccggcca tcacacgtac tggataggtc actgtttgca tggaatgacc ttcatagtag
     1501 tggaatctcc cttgcatggc taacactttc ttgcctgcca gagacccata aactagcttt
     1561 cctgcatgcc caactactgt cgaaacagga aagaacggga tctcatcata agaaatcatt
     1621 acagcatctt cgatttcatc tgctaattca cctaatcctg aacctagaat caaaccaaaa
     1681 tcaatttctt ttactccttt atcaaggata aactgggtcg tttcttgtaa catctcgctt
     1741 aacttggtca tttgctttct cctatttaaa aagattattt taataatttc aagaagcttt
     1801 cgccattttc agtcgaagaa acactaaagt tctcagcaat cgttgctgaa atatctgaat
     1861 aatatccttg aggcaatgcc ccttgttctt tcattttttt actgtaaacc aataacggta
     1921 catattctcg cgtatgatcg gtaccagtaa acgtcggatc gtttccgtga tcggctgtaa
     1981 tcattagcaa gtcgtcttct tccatcgctt caagcaattc tggaattcgt aagtcaaatt
     2041 cttcaatcgc atgcgcatag ccgactacat cccgacgatg accgtataat gcatcaaaat
     2101 ctactaaatt ggtaaagctg attcctgaaa aatcgttttt cataactttc agcagctggt
     2161 caacgccatc catattgctt ttcgtgcgga cagattcagt gattccttgt ccgttgaaaa
     2221 tatcgttgat ctttccaaca gcaatcacgt cctttcctgc ttctttcaaa gaatcaagga
     2281 ctgtgtgtcc gaatggattc aacgcataat catggcgatt gcttgttcgt gtgaagtctc
     2341 ctggttcacc cacgtacgga cgtgcaatga ttcggccaat catatacgga tcatcttttg
     2401 ttatatcacg aacgaattgg caaatccgat ataattcttc taaaggaatg atttcttcat
     2461 gtgccgcgat ctgaaggaca ggatcggcag aagtatatac aattaaatcg cctgttttca
     2521 tttggtgttc accgtaatcg tcaataacag ctgttccgct ataaggtttg ttacatacga
     2581 ctttacggcc agaaaattct tcgatttgtt tgatcagatc atcagggaat ccattaggaa
     2641 acacacgaaa tggagttttg atattcaatc ccatgatttc ccagtggcct gtcatcgtat
     2701 ctttccctac agaaacttct tctaatttcg tcgcatagcc ttgatgatct ttcactgatt
     2761 ccacaccttc aagaggacgg atcgttccta aaccaagctg ctctaaatgt ggaacgttca
     2821 atcctgcttc tttcgcaatg tgtcctaatg tatcagaacc aacatcaccg aacttatcgg
     2881 catctggtgc ttctccaatt cctactgaat ccatgacgat cagatgtacg cgtttaaaca
     2941 tgattattcc ttctttcttt ttatttttat tgcctttaaa acaaaaaggg cgagacgaaa
     3001 aacacttccc gtaacccacc ctttttattc gctaaaagat tattttgatt tgatatagtt
     3061 cttcccgttt gcttttggac cagaagcttt ccctaagaaa acaaccagca caatgattgt
     3121 cagcaaatat ggagcaattt gcagcaatac tggcggaatc gaagaaataa ctggtaattg
     3181 tgcgccaatg atactcaagc tttgagcaaa accaaagaac aatgaggcac ccattgcccc
     3241 taaaggattc cacttaccaa agatcatagc agccatggag atgaaccctt gtccaacgat
     3301 cgtaccagct gaaaagttac cggaaatcga ctgagcaaaa acagcaccgc ccataccgcc
     3361 taacaagcca gaaatcagta cgcctgaata acgcatagca tatacattga tacctaatgt
     3421 gtcggcagct tgaggatttt caccaactga acggaggcgc aagccgaatt tcgtcttgaa
     3481 gatgatcacc caagctagga cagcaatcaa aatagcacaa taagcaggta agaatgtatt
     3541 tttaaagaaa attggtccaa tgaccggaat atctgctaat cctgggaaag aaaaataccc
     3601 cagattctgc gtgatgttat cagtttgacc tttaccatac catgctttga tcaagaaaac
     3661 acctaaagca ggtgccatca gattaattac tgtcccgctg ataatatggt cagcacgcaa
     3721 actgactgtt gctactgcgt gaatcagaga aaataggata cctacgacgc cacccactaa
     3781 aatagcgatc catggtgtca tagaaccaag tgatggactc atcgtcaagt tgaaaacgat
     3841 ggaactaaaa gcacccatga ccatgattcc ctctagccct acgtttacga tcccgcttcg
     3901 ttcagaaaaa gttccgccta gtgacgttag aattagcggt gttgaataaa ccagtgtaga
     3961 agtaaggatc aaggcaatta attcgatagt cgacattata gttcgccccc ttcatggttt
     4021 tgattcgttg gtttcgattc tattgtcgca acgatctctt cttgtttttt cccttggaag
     4081 actttagcca tgacgaaacg aatcaggaag ttgatcccga caaagaagat aatcaaagca
     4141 atcgatacat tgacgatctc aaacggaaca cctgctcctg tctgcatgcc aagtccaccg
     4201 atcttcaaaa tactgaacaa gatcgctgat aaaagaatac cgattgctga tccagaacca
     4261 agcaaggaaa cagccattcc gtcaaatcca atagatagcg aagtcgtttg aacaaagaag
     4321 ttttgatacg ttcctaatcc ttcaaccaca ccgccaagcc ctgccaaagc gccagaaatg
     4381 accatcgaca aaacgatcgt acgtttacta ctcattcctg catactcaga agcaaaagga
     4441 ttcaatccaa cagaacggat ttcaaatcct aaagtcgttt tcttcatcag cagccaaacg
     4501 aaaataagcg cgatgattgc taaaaataat cctgagttta cgcgagagcc gccaaaagct
     4561 tgtgttaacc atggtaaccg cagtgaggca ttttgactga tcatattgga agagtccaaa
     4621 cttgagcgat agctttcagg catcacttca tgtaaagcaa atgtgcttac gtataataag
     4681 atatagttca tcatgattgt tacgatcact tcacttgttc caaaaaatgc tcgaagtaaa
     4741 cctggaacag ccgctgccat cgcaccagct aaggcaccta agatcacgca taacggaagc
     4801 aaaacaactt ttggcagatc tggaaacgca agtgccaccc agacactgac gatccacccg
     4861 caaagtgctt gtccagaaag cccaatattg aaaaacccag ctgagttagc tacagaaaaa
     4921 cctaatgctg taaaaatcaa cggcgttgct tgccttaacg tttcacctat ccctctttgc
     4981 gaaccaaggg ctgcatctag catagaagaa taaccttgga tgggctgaaa accaaaagca
     5041 gccatcaata tcgcgcctaa gatcaatcct aaaacaactg aaagtacagg cactaataaa
     5101 ttacggaatt tttcgctgcg atcattcatg tgcgccagcc cccttttcta actcttttcg
     5161 tgcttcttct aaggtatagc ccgccatcaa tagacctaat tcattttcag atgtttcttt
     5221 cggatcaacg ataccgacaa tttctccttc atggataaca gcaatacgat ccgaaacatt
     5281 taaaatttct tctaattcga aactgatcag taacactgct ttgtatttgt ccctttgttc
     5341 gatcagacgt ttatgaataa attcgatcgc acctacatcc agtccacgag ttggatttgc
     5401 aacaatcaac agatcaggat ctcggtcgac ttcacgagcg ataattgctt tttgctgatt
     5461 tccgcctgat aaagcttttg cgggaacaag ttcattcgtt gttcgaacat catattcttc
     5521 gatcaattct ctggcatgtt cattcattac tgaataattc agcatgccgt ttttactgta
     5581 aggtttttga tgataggttt gtagtgcgat attttcagat aatgtcatat ctaggactaa
     5641 cccgtattta tgacggtctt ctggcacgtg tcctacacca tgttcagtga tttttcgggg
     5701 ttttttatta gtgatgtctt ccccttttag cttgatatgt ccgctttctg cctttcgcaa
     5761 accagtcaaa gcttggatca actccgactg cccgtttcca tcgattccag cgataccaag
     5821 tacttcgcca gcacgaacct ctaagttcaa gtttttcacg gcttctaatc cacgattttc
     5881 tttcaccact agattttcaa tagacaaaac gacttcttga ggcttcgctt cttttttcat
     5941 cgttttgaat gaaaccgttc ttccaaccat catatcggct aattgttgtg aggttacgtc
     6001 tttaacgttg actgtaccga ttccttttcc acggcggata actgtacagc ggtcagctac
     6061 tgctttgatt tcatctaact tatgcgtaat caaaatgatc gacttgcctt ctttgactaa
     6121 ttccttcatg atcacgatta attcatcaat ttcttgaggg gtcaatacag ctgtcggctc
     6181 atcaaaaatc agtacatctg ctcctcggta aagtgttttt aaaatttcta cccgttgttc
     6241 cattccaact gaaatatcac gaacgtaagc atctgggttg actgacaatc catactgttc
     6301 agaaactttt tggatctctt ttcgtgcttt tttatggtca agcatccctg cgcgactagg
     6361 ctcgcttccc aacacgatgt tttctgttac agtaaaggca tcaacaagca taaagtgctg
     6421 atggaccatc ccaatcccta attggttggc tttggtcgga cttgtgatct ttacttcttt
     6481 cccacgcatc aaaattttcc ctgatgtcgg ctctaataat ccggaaagca cgttcatcaa
     6541 tgtagatttg ccagcaccgt tttctccaag caacgcatga atttctcctg cctttacttg
     6601 taaattgatg ttgtcatttg ccttgaatgt tccaaattct ttggtaatat tctgcatttc
     6661 tatgacaata ttttcttgaa ccaccttttt ttcacatcct aacgatttct atctagggca
     6721 acgattgact aagattcacg cttagtgagc gttcttcttt tgaaacaaac attacttcag
     6781 ggagttgttc ccttatccaa actgtcacaa ggcagcttgg ataaatgaac aattcttgtt
     6841 ctaatcaaac catagccttg tcaaacatgt gtctaacaag gctatagaaa tggttttagt
     6901 tttcttcagg agtttctgga acttttacgt cacctgaaat gattttttct tttgcttcat
     6961 caactgcttt tttcgcttcg tcagaaagtt gtccttctgt tagaccaaca ccgtcttctt
     7021 tcaatccata aacagtgtgt tcgccgccag ggaatttacc gtccgctgat ttttgtgcca
     7081 aatcttctac tactgttcca actgctttca gtgtagatgt caatgtgaag tttttctttt
     7141 cgccgtttaa tgtgtattcg ccttcgtctg attggtcgcg gtcaacaccg ataacccaaa
     7201 cttttttgtc gcctgattcg ttcaatgatt ttgcttcttg gaatacaccg ttacctgtac
     7261 cgcctgaagc atggaagatg atatctgcgt tttgtgcata cataccttga gcaattgaac
     7321 gtcctttatc tggagcagag aagtcaccag catattgatt caatactttg atttcttttc
     7381 ctaattcttt cgcacctgca tcaacaccgg ctttgaatcc agcatcaaaa cgatcgatta
     7441 cttcaccttt tacaccgccg ataaagccga ctacgtttgt ttcagtcgta taagctgctg
     7501 caacacctgc aagataagaa gcttcgttgt ctttgaaagt tgcagataca acgttatcta
     7561 atccatcgat gacatcatcg ataataacaa agttcgtacc tgtattgttt gttgcttgtt
     7621 cttggatcgc tggttttagt ttataaccaa taccaaaaat cgttttgaat ccagcattca
     7681 atgcttggtc aatatttggg atgtaatctg attcatttga agattggaaa tattggaagc
     7741 catcatttcc tcgtgaaagt ccttgatctt tcccccattt ttctagtcct tcccaagctg
     7801 actggttgaa tgaacggtcg tctacaccac ctgtgtctgt gatcaatgct gcagttgttg
     7861 tgtctgatga gcttttgcca ccggatgaat cagtagagcc gttgttgccg catgcaccta
     7921 aaaataaccc tgctgctagt gcgacagcac ctaaaccaaa taattttgct tttttcattt
     7981 ctgtaaagcc tcctaaaata attgttttat ttttcaacag tcagctgact gaatgaacat
     8041 gagttataga tctttttcag taaatgcata aggcagcagt gcacgaacgg tcgttgcctt
     8101 cgtcacacca ttgtctccta ctagcgtaac cggcatatca ggggaacaga attctgccat
     8161 tacttgtcga catgcaccac aaggagagat aggatcgggg gtgtgccccg ctacgactag
     8221 atgcgtaaat gttttttctc cttcagatac tgctttgaaa aaagctgtgc gctcggcaca
     8281 attcgttaat ccaaatgatg cattctcaat gttgatgcct tgataaatct tgcctgattc
     8341 cgttacgaga caagcgccta ccggaaaatg ggaataagga acataggctt tatctaacgc
     8401 ttcaatagct gtgttgatcc attcttgttt gagttctgcc atgagcttct cctttgaaaa
     8461 aattaatagc cttcaccagt tgaaccagat acgattgcta ctcctgcgct tgttccgatt
     8521 cttgtagcac cagcttcgat cattgccatt gcttcttctt cgttatggat accgccggaa
     8581 gctttgacac ccatttctgg tcctacagtt tcacgcatca aacggacatc ctctaccttt
     8641 gctccgcctg ttgagaaacc agttgatgtt ttgacaaaat cagcgcctgc agcttttgct
     8701 atttcgcaag ctcggatgat ttcttctcta tctaataacg atgtttcaat aataactttg
     8761 acaagcgcac gatcttttgc tgcttcaaca actgcttcga tatctttttg gacttgttga
     8821 tcatcctttg atttcaaagc accgatattg atgaccatat cgacttcatc tgcaccgtta
     8881 ttgatggcat ctgttgtttc aaatgctttt gtctcgggtg tgtttgctcc tagaggaaac
     8941 ccgattactg tacatacggc tactggctca ccttgtaatt gttctttcgc tagcgcaacc
     9001 catgctggat tgatgcagac agaataaaaa tggtattttt ttgcttcttc gataatttgc
     9061 atcacagctt ctttagatgc gtctgctttc aaaatagtat ggtcgatcat tcggtttagt
     9121 tccatttctt ttcccctttt ctttaaaacg gtaaagtccg cttttttcct ttattctgtg
     9181 atgatgtcat gaatcaacgt tggcttcatt gccgattcac caatttcaat atttttatat
     9241 aataattcaa tgacagaagt gatttcttcg tcgttactat agatcgtcaa tagtgactca
     9301 ccttttgtca cagtgtcacc aattttttta tgcagtttca atccaacagc gtgatcaata
     9361 tcttcttctt tggttttacg cccagcacct agcatcattg cagcaattcc taattcattt
     9421 gccacgattt ttgacacaac tccgctttgt ttagcaggaa gctccattgt gtagcgtgca
     9481 gtcaaaattc gttctggatg ttccacgatc gctgggtctc ccccttgatt ttggatcatt
     9541 tctttgaatt ttgctaacgc tttacctgat tccagagctt cttgaagcaa cgttcttgct
     9601 tcatcggttg tcttagcttt gccagcaaga acaaccattt ggcttcctaa ggcgtagcac
     9661 atttctacta gatcttctgg accatttcct tgtaatgttt cgattgcctc gacaacttct
     9721 agactatttc cgatagcttc gccaagaggt tctgacatat ctgagataac cgccactgtt
     9781 tctcggttag ccagtttccc aatgctcgtc atggtctttg ccagacgtct tgcatcttcg
     9841 atgttcttca tgaaagcacc gtctccagtg gtcacatcca aaacgattgc atctgctcct
     9901 gccgcgattt ttttactcat aatcgaactg gcaatcagcg gaagagaatc aacggtcgct
     9961 gtgacgtcac gcaaggcata caattttttg tccgccggtg ccaagtttcc tgattgacca
    10021 attaccgcta ctttactttc attaacaatg cgtacaaatt cttcatccga aagctcgatc
    10081 tgaaatccgg gaattgcttc tagcttgtct agtgtaccac ctgtgtaacc tagaccgcgt
    10141 ccagacattt tagccactgt cactccgaca ctagctacta ggggggctaa aatcaatgtg
    10201 gtcgtgtcac caacgccacc tgtcgaatgc ttatctacct ttattccttc aagagaactc
    10261 aaatcgatga catcaccaga attagcaatc gccaatgtta agttcgtgat ttcttcattt
    10321 gtcatgtctt gataaaaaat tgccatcaat aatgcactga cttgataatc tggaatcttt
    10381 ccattcgtat agttcgtgac gatgtactcg atttcctctt tcgacagctc gtttccatcc
    10441 ctcttttttt caatcagatc caccattcgc attgcctgat cctccttgtc ttcattcggt
    10501 attatacatt atttttagga aaggtaaacc actttagtaa actggtttac taaaaataaa
    10561 aaaagcacta actagccttt gtaaatgctt acataagcaa tataccgccg agttagccct
    10621 ttgtcaatac ttttacgctc tcacgaataa ttaattttgt ttcgaacact ttgttcggaa
    10681 cttttctttg aggaaattcg atcgtatcga tcaggaaacg tgcagcataa aatccaatat
    10741 caaaacttgg ctgctgcact gtcgtcaatt gcggaatcat gtattctgac agctttaatc
    10801 catcgaatcc tatgactgag atatcttctg ggattttttt gcccaattca tcgatcgcct
    10861 gatagcatcc gatcgccatg gcatcattcc cgcaaaaaat agccgtgata tctttatgtt
    10921 tgagtaattt ttttgatgca gtatatccac cttggacagt caactctcca tgttcaacaa
    10981 atttcccatt aaaaggcagg ccatgatctt tcagcgcatg acgatatccg ttgaaacgtt
    11041 cttctaattg atagtatcca gtggtttcct tcagcatgcc gatcttcgta tggccatttt
    11101 caataaacag cgaaacagct tgataggcgc cctcatattc ctttacgata agccgtccgt
    11161 tatcccgacg attcaatccg cgatcgatta aaatgattgg cattttttga taaaaaccgc
    11221 tgtctaatgc atatttctca ggtaaaatat ttggtgtggc cagcaagatt ccgtccactg
    11281 agcggtggga aagctcttgt aaatacttga tctcattttc ttggctatga cgagaattgc
    11341 ataacatgat cacatagccc aaaggattca aatagctctc tacaccttcg atcagtttgg
    11401 agaagaagaa atcagtgaca tcagggacga tcattccgat ggtctttgaa tgtcgattga
    11461 tcacatttga agcgaaataa tcgggcttat aatgatgttc attcacgatc gacaacactt
    11521 tttttcttgt cttatcacta aatctgcttc ctttgttatt caaaatctgt gagacagttg
    11581 ttactgatac gcctgccatt tctgcgatat ctctgatggt cagcttcatc ctattggctc
    11641 ccttctatct aaattcccta tcagtgtacc aaaaccccca agaaaagaat agagaaaaaa
    11701 ggaaagtatg ttttttgata aaaacctcct ttatctaccg acttggcagc cttctataca
    11761 agttctgtat cttatttatt ttatcgtaaa aaacaacgac tctgttccta ctatttcttt
    11821 ttgaagaaaa aatagttctt tgtttttaaa aacttctcaa caaaaaaaga actgatacgt
    11881 acccgtacat gcacgcccag ttccactctt tcgtcgctcc actatttttc agctagttat
    11941 tctttcacac ttttgatgat ataatatcct ttatcttttg tgacgatttc tgcattacca
    12001 aatgtttctt ccattttctt ttgcgcactc ggcgctcctt gtttcttctg gatcacaatt
    12061 gtcagtgtac cgcccttctt caaccgagga tatgcgcctg tcaaaatttc atgtacaact
    12121 ttttttccag cacggatcgg cggattgctt acgatagctg cataggtttc ttcatgaact
    12181 gcttcataga tattcgattg atggatatca acatgttcga tttggttacg ctttgcgttt
    12241 ccttgcgcta gacttaccgc tcgttcatta atgtcgacca tttcaacaaa ccgctgtgtc
    12301 gcaaatgcca aagctaaccc aatcggtcca tacccgcatc ccacatctaa gatctttcct
    12361 tcatcaggta actcttccca atcgaaagca tcaatcagga cacgtgaacc ataatccact
    12421 gtttcacgtg aaaatacacc agaatctgtt acaaattgaa aggttctgcc ttttaactca
    12481 aaagcccatt gttcaaaatc gtgcgccgta tctgggcgtt cagaatagta atgatttgtc
    12541 atgtcttcca tccttctcat ttctttcatg acaacagtct atccttttat gtatccgtgc
    12601 gcaagtcaag aataagactc cgtttctttc tattataaag attatacaaa aagatcggca
    12661 atctctctta agtggtgggg tttgcgttta ttttgtgtta aaatagttcc attgatggga
    12721 gaacgcagga ggctaatatg aatgatccaa tgaactacta ccgtgtggca agagaagaat
    12781 ggcgagaatt ttatcgtaat gggcaagcac ctttgacaca agatgaacta gataatatca
    12841 aaagtttgaa tgaccgcatt tccatgcagg atgttcgaga tatctatgta cctcttgctc
    12901 atctgattta tctttatatg aaagaatttg aatctttgtt cttgagtaaa ggtttatttc
    12961 ttcataaata cgtagaagta ccgcctttta ttatcgggat cgccggaagt gtagccgttg
    13021 gaaaaagtac aacagccaga ctgctgcaga cgattttgtc tcgtaccttc aaacgaagaa
    13081 acgttcagct gatcacgact gacggatttc tttatccaaa tcgtatatta aaagagcgtg
    13141 gaatcatgga ccgtaaagga tttcctgaaa gttatgatat ggagatgctg atcgatttct
    13201 tgaaccgtgt aaaatctggt caggaagaga taaaagtacc tgtttactca catgatgtgt
    13261 atgatatcat tccagacaag tacgaattga tccagcagcc tgatatcttg atcgttgagg
    13321 ggatcaatac gcttcaatta cctgcgaatc agcaaatcta cgtcagcgac ttttttgatt
    13381 tttctgtcta tgtcgatgcc gatcccgtgc tgatcgaaaa atggtatttg gatcgttttg
    13441 gtgcgttact ggatacggct ttccaaaatc cagagaatta ttattatcgt tttgctatcg
    13501 gagatcgtga agaagccttt aaaatggcaa aagatgtatg gaaaaatgtg aatttgaaaa
    13561 atttacagga atatatcttg cctacacgag gaagagccga tataattctc cataaaactg
    13621 aaaatcatat catcgattcg attttcatgc gaaaatatta gcattttaaa atagtttgac
    13681 tattttgaga taaaatttaa agggataggg gtaataaatt tgacaaacgt tgccgatatg
    13741 acgaacgttg aaaagattat cgtattagac tacggtagtc agtataacca attgatcaca
    13801 agacgtattc gtgatcttgg agtcttttcc gaattattga gccaccgcat tactgcagaa
    13861 gaagtaaaag cgatgaatcc aaagggaatc attctttctg gcggaccaaa cagtgtatat
    13921 gatgagaatg catttgggat cgatcctgag atcttcaatt taggtattcc tgtattaggg
    13981 atttgctatg gtatgcaatt gatcacgtac aaacttggcg gttccgtaga gcctgctaaa
    14041 aaccgcgaat atgggaaagc cttgttagaa gtaacaagcg atcaagctgt cttgtttaaa
    14101 gaaacaccaa aacaacaaac agtatggatg agccatggtg acttggtaac taaagttcct
    14161 gaaggctttg aaacagtcgg aacaagcaaa gattgtccga ttgcttctat ccaagacaca
    14221 acacgtaatt tttatggtat ccaattccat gcagaagtac gtcattccga atacggaatc
    14281 gaattgctgc gccactttgc ctttgatgtt tgtcactgtg aaggtaactg gacaatggat
    14341 aactttatcg atatgcaagt agaaaaaatc cgtgaacaag ttggtgacaa aaaagtcttg
    14401 cttggtcttt caggtggcgt tgattctagt gtcgttgggg ttcttttaca aaaagcgatc
    14461 ggtgaccaat tgacatctat ctttgttgac cacggtctgc tacgcaaagg cgaagcggaa
    14521 caagtaatgg aaagtctagg cggaaaattc ggcttgaaca tcatcaaagt caatgcaaga
    14581 gatcgcttct tagacaagtt gaaaggtgta tctgacccag aacaaaaacg taaaatcatt
    14641 gggaatgaat tcgtatatgt ttttgatgac gaagcaacaa aacttgctgg tgaagaaggc
    14701 gtctctttcc tagcacaagg aacgttgtat actgacgtta tcgaatctgg tacagaaaca
    14761 gcacagacga tcaaatctca ccataatgta ggtggactgc cagaagatat gcaatttgaa
    14821 ttgatcgaac cattaaacac attattcaaa gacgaagtcc gtgaattagg gactcagtta
    14881 ggcatgcctg attcaatcgt ttggcgtcag ccattccctg gtcctggtct tggtattcgt
    14941 gttcttggcg aaatcactga agacaagcta gaaatcgtac gcgaatctga tgctatcttg
    15001 cgtgaagaaa tcgcagctgc tggacttgac cgtgatatct ggcaatattt cactgttctt
    15061 ccagggatcc gctctgtcgg tgttatgggt gacggacgta cttacgacta cacagtcggt
    15121 atccgtgctg taacttcaat cgacggaatg accgctgact ttgcccgtat cccatgggaa
    15181 gtcttgcaaa aaatctctgt acgtatcgta aacgaagtag cccacgtcaa ccgtatcgtg
    15241 tatgacatca cttctaaacc accagcaaca gtggagtggg aataaagagc caacataata
    15301 tggaggggga atccctataa atcaacattc ttcaatagct aaaatgcttg attttcatta
    15361 aaaataagat ggttccctac caaaagagac tataatataa gttaatttgt ttcattctta
    15421 tcataaaaag aagttctcga aaaacgaact gttttccgag aacttttttc tatccatcta
    15481 agtgctcaaa tcatattcac gacggaattc taaggttacc ctttcttaaa caaactttat
    15541 gttatctgca caactttttg caattttttc attgcttctt acgaaatcgt tgaactctct
    15601 ttagcataat acaaagtgta gatgaagtct ggtgctttac tcgttttggt aaaaatgact
    15661 ggaaatcgtt tctggttatt tttggcaaag gcgaggtgca ggttcgattc ctgccttcta
    15721 cgctaacgat gtggctgaag ggaacctccg atcttgaatc gaaacgatcc gcagtctatt
    15781 gtttcaccaa tgaaacaaaa tcaacgttcg tccaagcatt tcctgaagcc ttgctataaa
    15841 ccccaatttc acaaacacct gttgtaattg gaatgtcttt gattgtaatt tttgtccatt
    15901 cacttgtttt aacaaccgca gtatttagtt ccgctccacc ataatttttg atatataact
    15961 gattagtatc aaactctccc ccagacatta cataggcact caatgtgtaa gttcctttgc
    16021 ttatgccact aacattttga taagctgatg cctcgtatgc ttgatcctgc caaaaagtta
    16081 gtttgtagtc cccaaaaaaa gtgcccgctt ccgttttgat cgtaccctta gcggttcctt
    16141 gttgaaacca ggtttgccaa ttggaaggac tggtcgttgg tccatcgctt tcgaaacttg
    16201 catttttaac tagatttatt tgcgcccctg cttgtttaaa cgcacgcaag cttggcaagg
    16261 cgtttccagc aaaatcaaac atcgcttggt tgtcccattc gtttcccata gtggattgaa
    16321 cactgagata attcatacct gcttgtgttg cccaactcac atttccgttg taccaaagag
    16381 gttcccaata gaagaagccc gccccgcgat tattcggtac tgcctgaatc cgatcggcaa
    16441 gatccagcaa atactctgct tgtcctgtcg gtgttggttc atatcccccc gcagctgctt
    16501 cggcttcacc aaaagcattt ggcatagtat cggcattcgc tgttgtatgc ccataagctg
    16561 tttcaactac gatcacttct ttaccatacc gttggctgat cgcattcata ttggtttgaa
    16621 gttcattcat tgaaccgtgc caatacggat aatacgacac tccaattaaa tcaaatgaca
    16681 cacctcgact cgtgatttca tcaaaccacc aagtaaatgc tcctgtatcg ccaccatcag
    16741 ctaagtggag cattactgga gttgttttag gttgagtgtc tcgaacagct tggataccag
    16801 acttcaaaaa agcagccaat cgatcaaatt ctcccccgcc ttctccccaa gacttgccat
    16861 aaggccagag cattccgcta ttcagttcat tgccgatctg aaccatatca ggataaacgt
    16921 ccagctgctt catttccgac agaacgttct ttgtatagtc gtacaatgcc gtattcattt
    16981 cttcaaaggt cagaccttgc catgctttag gaagattctg tttcccagga tcaacccaaa
    17041 aatcactgta gtgaaaatcc agcagcacct taagtcccag attttttgct cgttttgcaa
    17101 ggtcaatcgt cgtttccaga tcatttgtcc ctgctccata actttctcca ttactattat
    17161 aaggatcctg ccacaaacgc agtcgaacat agttgacacc attatttttt aaaatctcta
    17221 aggcatcttt tgaaatatcg tcttcataat attttgcacc agatttttcc atatctgaca
    17281 ggatcgaaat atctgctccg cgaataaatt cgctggcaga tgcttcaaca gtttttgaaa
    17341 cagcccccaa agcccctaac acagctgcaa ataaaacagc tttacgcaac caaattccca
    17401 ttttcattat ggctcctcct ttattgaaat tggttcagaa taaactgaac agctttttca
    17461 ggatctctcg ttaatccgat atattctgct ccttttgtcg caaccagctt gattcctaag
    17521 cgatcggtgt cttctttgat atcatcatag taagaattga cttgcggtgc taagaccacc
    17581 atatcaaaat cctgcagcat ttcgtaatga gaaccataag cacccgccat tgcactgatc
    17641 ggtgctttcg attcagatgc accttttttt aaagcattag ctaacatccc acttgtccct
    17701 gcgccagcac aaagtaccaa cacattgatt tctttatttg catcgctgat aacttgactc
    17761 gctgccacag gtacttcctc acgtacatcg gcagaagcac cttcctcggt tgcgatcacc
    17821 tctgcttttt gtgcttcttt ctctactaag acagcatcgt atgctttgca aaatggtaga
    17881 tagatcagga agtcaacgac gattagaacc gctgctagaa caaaagctaa gatacccatg
    17941 cccgttccta gaaccaaacc gatcggtgct ggagttgccc aaggcaaaac gtacataaag
    18001 ctgttcatgc ccaatacatc gacaaaaaac ttgaacaggc aaacgtttac aacaggtgtt
    18061 aacaaaaatg gaataaaaaa ataagggttc aaaatcatgg gtgccgcaaa tagcaatggt
    18121 tcgttgacag cgaaacttac tggaataaag gaagctttcc caactgcctt caactgctta
    18181 gacttagcaa agagtaggaa gataaaagga acaaccaatg ttgccccagt tcctcccata
    18241 gttcctacaa aattccctaa acccaccgtc agtactttag aagcttgttc accggaattg
    18301 aacaattgca gattggtttc cacattggca tagataattg ccgcaattgc cggctccacg
    18361 atcgacggtc catgaacacc gacaaaccaa aacagcgcca tcgctcccca gataatagcg
    18421 attcctaagt acccgtcagc tgctgtaaat agcggctgca gcaccgtaat gattgcttct
    18481 gcaaatgaga tttggaagat ttgtcgaatg cctaaatcaa ttaaaacggc cgcaaatact
    18541 gaaaaagaga aaggaaaaat atctcggaag gtttgcgaaa tcgttccagg aacttcttta
    18601 ggcattttaa tggtaatatc tttcagcaca caaaacttat ataaattaac tgttaaaaat
    18661 gcagcgacaa aggaagccag tagtcccttt gtccccatat attccgttga aaatccacct
    18721 tcaatcggag agacgcttaa caacaggaaa cttaccacag aggccagcat cacagaggtg
    18781 gtgttgatta ctttgttttt cggtaagcga cgattcatcg aatcagccaa gcagcgagca
    18841 gtggttgctg aaacgagtaa ccctacaaca cccatcgaat aattatagac tttccatagc
    18901 catgtggaga cagattcagg ccattggtaa ccaaaaatct caggaaccgc agcaatcaaa
    18961 atgaaaatgc ttgaaaataa aatagctggc atcgctgtta aaaatccgtc acgaacggct
    19021 cctaaataac tattacgtga gattttttca aaaaatggtt tccctttttc gatttgttta
    19081 atgattccat ccatagcgtt cttctcctat ccttgattct gtctgtacag tacaataaag
    19141 tcttgaatca gatctctcag caataaagtg gtcattaaat gatcctgccc atggataaaa
    19201 atgaatccca tctccatctc tttcccagat gcttcttccg ctaaaatttt tgtttgcgaa
    19261 ttatgtgcaa gggttaaatt ttcgtcggct tctttcaacg ccgaatcaac atgttcaaag
    19321 tttccttgtc ttacttcttt caataatttc aataatgtac tgcgagcgtc gcctgaatat
    19381 gcaacgatct caaaaccaag catttgtaat tcctctttat tcatcttttc ttcctccatt
    19441 atcacttatt aaataatcgt tttggtttca ctgaccaact tgtaccagta agctgattct
    19501 tttgggtaac gcttctgggt ttcaaaatca acataaaaca agccatacct tttgttatac
    19561 ccatttgtcc atgagaaaag atccataagc gaccacaaaa aatacccctt cacatttacc
    19621 ccggctgtta tcgccttgct taaggattcc aaatagactc tcaaatagtc gattctcggc
    19681 tgatccataa taatgccatc ttcaaactga tctttatacc ccatcccgtt ctcagtgata
    19741 tagattttgt tgtaatgtgg gtaatcgctt ttaattcgca gcaacaagtc atatagacct
    19801 tctggataaa tcagccagtc ccaatcagtt cgaggaattc cttctttata aatacgttct
    19861 ccgattccct tcactttata aacagaggtc cctttttcac ccgtaccgtt atgatggatt
    19921 gcattttctc catcgtaagc tttgacaaaa tggcattggt agtgattgat ccctaagtag
    19981 tcattacgcg tggatgcttt tttcaattca acgaaatctt cttcaggaaa atgatagctg
    20041 gcctgatttg cttcacatat ctcatcaaga gcagtcaagg tttcagttga ataatagcct
    20101 aaataggtag catctaataa gaaacggatc gacaatgcat cgtctaaaaa ggcagcatgc
    20161 ttatcttccg gcgcgtctgt tgccgcatat ttcgtctcta atgagtgaac tacaccaatt
    20221 tcacccggaa gttcgttttc tttgaaatag ttcactacac gggcatgagc aaccatcata
    20281 ttatgaagac aggcgacgat ctttgtaaaa tcatatttga ttcctggtgg gaaaacacct
    20341 aacaaatatt gattggttgc aactggatag atttcattaa aggtactcca caccttgact
    20401 tctttgaatt catgaaaaca aaaaatggca taggaaacaa atgcttctat tgtttctcga
    20461 ttcaaaaaat ctccatggtc aaacaaacgt ttaggggtat caaaatgatg caacgtcaca
    20521 aacggcgtta cgtttctttt cttacattct tcgaacacac gatgataaaa agcgattcct
    20581 tctggatttg gtttcccagc tccatcagga aaaatgcggc tccaagcaat ggataatctt
    20641 agaccattta caccaaaacg ttcacacaat tcaatgtcct ttgcatattg atgataaaaa
    20701 tcacttgccg gatcgggact gaatcgccct tgttcctcta aaaagtcatc ccaagcaaca
    20761 gcgcctttgc caccttcctt agtggctcct tcaacctgat aggctgctgt ggctccgcca
    20821 aaaataaaat cttctggtaa tttaagcatg tacatctctc ctcaaatcta taatcagttt
    20881 tcgctctttc taactgattg cattgtaaac gcatccaaaa catttgtcaa caaaaacaaa
    20941 cacaaataat cataaaaacc agaaaacagc aataaaatct attgtaacaa acataaaaac
    21001 caccctaaat gtttactttt gttcgaatta gattgactgt tgtccgttta tatgatagat
    21061 tatccttaaa ttagaggtga agacaatgtt gaaaaaagaa agattgctta ctatcgtaga
    21121 aatggtcaat aaaaaaggaa tccttaccgt taatgaaatc atcaatcaat tagatgtttc
    21181 tgatatgacc gttcgcagag atttagatga attagaaaaa tcgggcaagc tgctgcgtgt
    21241 ccatggaggg gctcaaagtc tttcctacac tttagatcaa gaactttctc acactgaaaa
    21301 agccactctg cagattcaag aaaaaaaaga aattgctgcc acagcagcct ccttgattca
    21361 cgagggagaa acgattttct taggaccagg tacaacaatc gaactattgg cgacgcattt
    21421 actgaacaag aaaattcgga tcattacaaa taattatcca gtttttgata ttcttgctca
    21481 agcagatgga ccagaaattc tactgattgg cggtgatttc cggaaaaata ccggcgcatt
    21541 tgtcggaccg atcaccaacg acaatttaag aagactaaag tttacaaaag catttatcag
    21601 ttcaaatggc gttcacaacg aagcgatctc tacttacagt accgaagaag gagaagcaca
    21661 gcagatcgct ctaaataatt cccgtaagaa atatctactc gttgataata aaaaattcaa
    21721 tcgagatgat ttttatgtgt tttataattt gcatgatttc gatctgctca ttacggatcg
    21781 ccagatctcg cgggaggttc aagagcacta tgcacaatat gtcgatctgc aaattgctga
    21841 ttcaaccgaa aataccacta taggagcgta actatgaaag ggattatatt tgatttcaac
    21901 ggaacaatgt ttttggactc tcatctgcat gaagctgctt ggctgcatat gatacatgat
    21961 catacaaaag atggtttatc agacgaggat attttaaaaa atattcatgg tcggacgaat
    22021 acggagattt tgaaacattt catttcagag catttgaccg cctctgagat cgccgtattg
    22081 tcagaagaaa aagaaagcta ttatcgtatg ctttgcttgc aaaatgaaaa cgagcttcag
    22141 ttgacaaaag ggcttgaagc gactcttgac cagctgactg accaacagat tccattaacg
    22201 attgccacgg ctactgtcaa agagaatgtt gctttttatt ttgatatttt cgatttagcc
    22261 cgctggtttg actgggacaa gattgtctat gacgacggct ctttccctgg gaaaccgcaa
    22321 ccggatattt ttcttaaagc ggcagacaaa ctgagcttag ctccggctga ttgtcttgtt
    22381 atcgaagatg cttactcagg attacttgct gccaaacgtg cggggatcgg aacaattatt
    22441 gcaattgatc cattcggtaa aaacaaaaca gtatttgaac aggcacagct aaataaagat
    22501 ggaaccatca aagattttac tggtttttgg acagagcacc tgtctgccac atccgttaat
    22561 tcttaaacga ttcaactaac taccaaagaa aagcaactga aatgcacacc tccttttata
    22621 aaaaactgcc agtgaaacga catgaaagat acactgctat atccttagtg aaatatagca
    22681 gtgtatcttt gactttttca ttcttttaac catatgacgc gttcatctct atctataatt
    22741 aacattaaaa atagtgtttt cacattattt ctcaaaccaa tcttaaaaat catcaggagt
    22801 acttttttga tatactttta ttaatgctgg gcgtaatggt taaaagattt ccactagttc
    22861 ctctccacca agctgagttg aaaaatgctt atctgtaaac aacatagaca aagaggaaat
    22921 aaacgtttcc gtatctttct ctgtcccaag gatattaatt aattttccta ctaggtaagt
    22981 atcattaaac ttaagatctg ccttcacatc aaaagttttg ataaaagctt ttatcatgcg
    23041 tacaattttg tctttatcgc cataattgat ttcaccactc atatcaatat agtcactaat
    23101 ccctgaagaa ataatggcaa ctgcttcttc tttcgcctta tcagataatt catttaatga
    23161 ctgggattga ttgatgactt cttgagtttt atctgttacg taatctctca ttatcctatc
    23221 ggcatactcc gcaaagtaat cttgaaaaaa ttcttctaca gggtaaccaa cttcgttttc
    23281 ccaaccagga gaaatttcat tatcagtact tattaaatct attaaaaacc aacccgttct
    23341 ctgagtgttg attctataat atttttaatt tcctatataa atcaaaaata ttatagaatc
    23401 aataagctaa ttaagaaaca aactttagaa actctagaag aacttctgaa tttatataga
    23461 agtgtgtagc tacttactaa atatacaaaa gttaaaataa aatactctgg atactctt
//
LOCUS       DASJUF010000040        23198 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJUF010000040 DASJUF010000000
VERSION     DASJUF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.040"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1633
                     /locus_tag="U4B62_001391"
     CDS             <1..1633
                     /locus_tag="U4B62_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2141558.1"
                     /translation="AVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFNLVVPQEMPTA
                     DLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTYKIKLREDAKW
                     SNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKSELGIKAVGDY
                     ELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNGPFTLEDFDGP
                     GTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDVILSGELAQQN
                     ANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVKQVLGDGSVVS
                     TGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDSLEFDLMASDD
                     DSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGGWAADYADPSS
                     FTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIKIISDDMGVVP
                     VYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1703..2050)
                     /locus_tag="U4B62_001392"
     CDS             complement(1703..2050)
                     /locus_tag="U4B62_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN2141559.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2134..3075
                     /locus_tag="U4B62_001393"
     CDS             2134..3075
                     /locus_tag="U4B62_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141560.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3076..4119
                     /locus_tag="U4B62_001394"
     CDS             3076..4119
                     /locus_tag="U4B62_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141561.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4125..5195
                     /locus_tag="U4B62_001395"
     CDS             4125..5195
                     /locus_tag="U4B62_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141562.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5185..6138
                     /locus_tag="U4B62_001396"
     CDS             5185..6138
                     /locus_tag="U4B62_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2141563.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6150..7160
                     /locus_tag="U4B62_001397"
     CDS             6150..7160
                     /locus_tag="U4B62_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2141564.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7445..8266)
                     /locus_tag="U4B62_001398"
     CDS             complement(7445..8266)
                     /locus_tag="U4B62_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2141565.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8363..8641)
                     /locus_tag="U4B62_001399"
     CDS             complement(8363..8641)
                     /locus_tag="U4B62_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141566.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8909..10918
                     /gene="metG"
                     /locus_tag="U4B62_001400"
     CDS             8909..10918
                     /gene="metG"
                     /locus_tag="U4B62_001400"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN2141567.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11397..11984)
                     /locus_tag="U4B62_001401"
     CDS             complement(11397..11984)
                     /locus_tag="U4B62_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141568.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12159..13481
                     /locus_tag="U4B62_001402"
     CDS             12159..13481
                     /locus_tag="U4B62_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2141569.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13636..14412
                     /locus_tag="U4B62_001403"
     CDS             13636..14412
                     /locus_tag="U4B62_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2141570.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14405..14974
                     /gene="rnmV"
                     /locus_tag="U4B62_001404"
     CDS             14405..14974
                     /gene="rnmV"
                     /locus_tag="U4B62_001404"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN2141571.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            14967..15851
                     /gene="rsmA"
                     /locus_tag="U4B62_001405"
     CDS             14967..15851
                     /gene="rsmA"
                     /locus_tag="U4B62_001405"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN2141572.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16302..16931
                     /locus_tag="U4B62_001406"
     CDS             16302..16931
                     /locus_tag="U4B62_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141573.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17055..18164)
                     /gene="ugpC"
                     /locus_tag="U4B62_001407"
     CDS             complement(17055..18164)
                     /gene="ugpC"
                     /locus_tag="U4B62_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN2141574.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18373..18795
                     /gene="mgsA"
                     /locus_tag="U4B62_001408"
     CDS             18373..18795
                     /gene="mgsA"
                     /locus_tag="U4B62_001408"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN2141575.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19118..19654
                     /locus_tag="U4B62_001409"
     CDS             19118..19654
                     /locus_tag="U4B62_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2141576.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19694..21457)
                     /locus_tag="U4B62_001410"
     CDS             complement(19694..21457)
                     /locus_tag="U4B62_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141577.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21460..23175)
                     /locus_tag="U4B62_001411"
     CDS             complement(21460..23175)
                     /locus_tag="U4B62_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141578.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7446 a         4267 c         4735 g         6750 t
ORIGIN      
        1 tgcggtatgc ggccttgtac ttgctggatg ttatggcggc agttcagcca caagcagtag
       61 ttcagcaaaa agcggttcag cagatggagg aggcgtattt aacttagtcg tgcctcaaga
      121 aatgcctaca gctgacttat cagttgcaac agatacaatc agttttacag cattgaataa
      181 cgtttatgaa ggaatttacc gtcttgatga agacagcaaa ccacagccgg caggagctag
      241 cgagttggca gaagtcagtg aagacggatt aacttacaaa atcaaattac gagaagacgc
      301 aaaatggtca aatggtgatc ctgttacagc agcggattac gtttatggct ggcaacgaac
      361 agtagaccca gctacagctt ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga
      421 agacattaca gctggaaaga aagataaatc agaactaggg atcaaagcag ttggtgacta
      481 tgaattagaa atcaaattga cgaaacaaac accatatttc caatatctgc ttgctttccc
      541 atcatttttc ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct atgcatctgc
      601 aagtgataaa gctgtttata atggaccatt cacattagaa gattttgatg gaccaggaac
      661 agatacagaa tggacttata agaaaaatga tcattactgg gacaaagata cagtaaaact
      721 ttctgaaatc aaagtcagtg tagtaaaaga atcttctact gcattgaact tattcaaaga
      781 tggccaagca gatgacgtga tcttgtctgg tgagttagca cagcaaaacg caaacgatcc
      841 agcttatact tctgttaaag aagcgcggac aagctatatc gaattcaatc aacgtgaaaa
      901 gaattcacca ttcaacaatg tgaacctaag aaaagctatt tcttattcta tcgatcgtga
      961 agctttagta aaacaagtac taggtgacgg atcagttgtc tctacaggat tgatcccatc
     1021 agatatggta aaaagtccaa caacgaacga agactttgca aaagaagctg gaaaattagt
     1081 ttcttatgac aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt
     1141 tgattcttta gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga
     1201 atatgttcaa aactcaatcc aagaaaactt ggatggcgta acagtcaaac caacacctgt
     1261 tccattctct gtacgtcttg accgctcaac ttcgggtgat tttgacgcag tactaggcgg
     1321 atgggctgct gactatgcag acccaagcag ctttactgat ttgtttgtca caggtaattc
     1381 atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag cagcttcatc
     1441 aacagacgct ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa tcaagatcat
     1501 ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga
     1561 aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca tgggattaca aatggacata
     1621 cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc
     1681 gcaacctcgt tctcatattc acttatccta acaaagaaaa aagcatgaaa atagcagaaa
     1741 gagcaattag tattccggca atgatcagcc aaaagctgta cataccgtag ctggcagacg
     1801 agagcgagga aaattctggt tttttctttc ggttccttgt tcgtgcaaga tggaccgatc
     1861 gctgaaaatg atcgaagaaa cccgaagaaa agacccataa aaaaccgccg ataatcagaa
     1921 acggcaatga ccataagaat agatcattag ataattgaat gagtgtaaag gaatggttga
     1981 tgagattttt tattaaaata accagaatca gtagaaaagc tactgcgtag gggatagatt
     2041 tttttttcat ttgattgctc ctttattttt ggttcattat ctaataatgt agcgaaacat
     2101 ctgtcagaag tcaaaagcta gggggaaaca ttagtgaata gttatatcaa atatgtatta
     2161 aaacgtgtct ttttcatgat catcacgctt tggttaattg caacaatcac ctttttcttg
     2221 atgcaattgc tgccgggcac gccatatacg aatcaggaac gtttgagtcc tgaaacaatc
     2281 gagatgctaa ataaacaagt cggtttggat aaaccagtta tcgtacaata tgggatttat
     2341 ctttctaatt tgcttcaagg agattttgga atttccttcc aatttaaaaa ccagccagtc
     2401 gcacatttgt tagctggaag aatcggacca tccttgcagt tagggttaca ggcaattatc
     2461 tttggaacag tcctagggac gattttagga acgatctctg ccatgaagca aaatacatgg
     2521 gcagatacat cttctacact cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt
     2581 gctgttttat tacaatatat ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg
     2641 gatggctttg tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac
     2701 tctgctcggt ttatccgtac ggaaatggtg gaagtactgc atagtgatta tgtggaatta
     2761 gcaaaagcaa aaggattgag ccgttgggaa atcgccttta aacatggtct tcgaaatagt
     2821 ttgattccat tgatgacttt attaggaccg ttagctgtag cattgatgac cggatcactg
     2881 gtggtagaaa atatctttgc tattcctggt atcggggaac aattcgttaa gtcaatcaca
     2941 acaaatgatt atcctacgat catggctgta acgatccttt actcatttat gctgattttt
     3001 gttattttga tcgtggatct attgtacggt ctagttgatc caagaattcg tgtttcagaa
     3061 gggagccgag gataaatgga aatgatacca aagcgttatg aaacagttgc agatatccct
     3121 gcggatgaat ttcttccatt acaaaaaaat accgaagaag aacgtgaaca gattactgca
     3181 ccttctttga attttatcca agattcatgg cgccgtttaa agaaaaacaa agctgccgtt
     3241 atttcaatgg ttttactatt ggtcatcatt ttgatctctg tgatcacaat ctttgtttct
     3301 cctcatgatc caactgccca aaacgtggct tatatcaatt tgcctcctcg tattccggga
     3361 atcaatatcg atggattgaa tggaaaagca atggttggtg gtgaacttgt tgataaatat
     3421 gctcaagcaa acgtcccttc aaacgtaaat ttctttttag gaacagacgg tttgggacgt
     3481 gatgtattaa gccgcttatt tatgggaatg agaatctcgt tgctgatcgc atttattgct
     3541 gcattactag atgtaacgat tggtgtagct tacgggttgg tttctggttt gttaggcgga
     3601 cgtgtcgata acgtgatgca acgttttttg gaaatcttat caggaattcc aaacctcgtt
     3661 gtcatgattt tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg
     3721 gccatcacaa actggatacc aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa
     3781 gatcaagaat atgttttagc tggattgaca ttaggtgaat caagaatgaa aattgcattc
     3841 aaacatattt tgccgaatat ctctagtgtc attattattc aaatgatgtt tagtattcca
     3901 tctgctattt tcttcgaagc ctttttaagc tttatcggtt taggtcttac gcctccgtca
     3961 gcttcattag gaacgatgtt gagcgaaggg tacaagacat tcctgtatct gccttatcta
     4021 ttatggattc cggcagtaac tttatcggtt atcatgattg gctttaactt acttgctgat
     4081 ggattgcgtg atgcctttga tcctaaaatg aaagagtgaa gacgatgacg aaaattttag
     4141 aagtgaaaga cttagaaatc tcttttgata cgtatgctgg aaaggtacga gcgattcgcg
     4201 gtgtcaattt tcatttgaat aaaggagaaa cattggcgat cgttggggaa tctggaagtg
     4261 gaaaatcagt gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc aatatagatg
     4321 ctggtcaaat cctctttaaa ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa
     4381 aaatccgtgg taaagaaata gcgatgattt tccaagatcc aatgacctct ttagatccga
     4441 cgatgcccat tgggaagcaa gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa
     4501 aagagggaca aaaagctgca ttagaattgc ttaaattagt tggaataccc gaagctgaga
     4561 aacgtatcaa cagttatccg catcagttct caggaggtca gcgtcaacgg atcgtgatcg
     4621 cgattgcact ggtatgttat ccggaaatac tgattgctga tgaacctaca acagcgcttg
     4681 atgtgactat tcaagcacag attttggagc tgctgaaaga aattcagaca aaaatcgata
     4741 catcaattat ctttatcact catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg
     4801 ctgtcatgta tggaggaaga atcgttgaag ttggaacatc ggaagaaatt ttctacaacc
     4861 cgcaacatcc ctatacttgg ggactgcttg gttcaatgcc gacattagat agtgcgaacg
     4921 atcgattgta tgctattcct ggttctccac cggatttatt gaacccgcca aaaggagacg
     4981 cattctatcc aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt
     5041 ttgaactatc aaaaacacac aaggcagcaa cttggctact tgctccgcag gcacccaaag
     5101 tgacaccgcc agaagaaatc caacgtcgct gggctatctt taaagagaaa caaaaacata
     5161 cttacggtca aggaggaatt gctgatgcca aataaactac ttgaagttaa aggattgaaa
     5221 cagtatttca atgtaggaaa aaagaatgaa gtacatgctg taaatgacat cagctttcac
     5281 atttatgaag gagaaacatt cggtttagtt ggagaatctg gaagcggaaa atccactact
     5341 ggccgtacga tcattcgctt gaatgaacct actgatggtg agatattatt cgacggtcaa
     5401 gatataacga agatcaaaga taaaaaaggt ttgacgaaat tccggcatga tgttcagatg
     5461 atctttcaag atccatacgc ttcgttgaat cctcgtatga aagtcagaga tatcattgcc
     5521 gaaggaattg atgttaatgg actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat
     5581 gatctattga gaactgttgg attgaatcca agccacggta ctcgttatcc tcatgaattt
     5641 tcaggtgggc aacgccagcg tattggtatt gcacgagcat tggcagtaaa accgcgtttt
     5701 atcatttgtg atgaaccaat ttctgcacta gatgtgtcga tccaagccca agtggtcaat
     5761 ttgttgcaag atctccaaaa agaacatcaa ttgacctatc tatttattgc acatgatttg
     5821 tcgatggtta aacatattag tgaccgaatc ggtgtaatgc ataacggctt gttattagag
     5881 atgggaacaa gtgatgaaat ctataatcac ggtgttcatc cttatacaga aagtttgtta
     5941 tctgctattc ctctaccaga tccagaccat gagagaaaac gcagaagaat caaatatcaa
     6001 cctgaaccag atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc
     6061 tatgcaactg agcaggaagt cccatattac gcaaaaaaac taaaacgaca aaaggaatct
     6121 cttctggttg ctaactagga gggacataga tgctaaaagc ttttaaattc cgtatgtatc
     6181 cgacagaaga acaaaaacag caattgattc gtacttttgg atgtgctcga tttacctata
     6241 accatttgct gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc
     6301 tcacacccgc tacattaaaa aaagaatacc cattcttaaa agaagtagac agtcttgctt
     6361 tagcaaatgc acagcttaat ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca
     6421 gcttcccaaa gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa
     6481 cccgtacgat ctatctggaa aatggctact taaagttacc aaaacaaaaa gaactaatca
     6541 agataaatca gcatcggcca gttgaaggat cgattcgctc cgctacgatt tctgctcgat
     6601 ataatgaaga attttatgtc gcactacttt gtgatgtttc gccagtaaaa aaagaatctt
     6661 tagctaaatg gatcgggatc gcctatcatc cgaagacatt gatccaaaca tctcgacctc
     6721 tagaagtaac tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat gcacagcgga
     6781 aattgaatgt gaaagtccgc tccgctcacc atagaaagat caggttagat caggcaagca
     6841 attatcagaa acaaaagaga aaagtaatgg atctttatct gaaacaaaag aaccagcgag
     6901 aagattatct tgaacaatta tctgggaaat tggtcaaaca atatgactac ttatttgtag
     6961 aatcttttcc aaaagaagag gcacatgctg atttttctat tcacgactgg cataaattaa
     7021 taacaaaatt gcgttataag tcccaatggt ataataaaaa atttcttttg atcaataccg
     7081 acggagcaga agaaagcaat tccgtaagaa agagccaagt agtagaaaaa atgggcagac
     7141 attcggttat caagggataa aaaatgaatg aaactgatag gagcgttagg gttagccttg
     7201 ttaataaaaa gtcttaatga cttttgtttc taggaatttt tcttttgaag aaaagctcac
     7261 taattatgac aaaccaataa aacaatagca gaaaaagttc gtacgagctt tttctgctat
     7321 tgttaatttt ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa
     7381 ctcacaaaga gttgtttttg cgttttttct tgaatgatta ggtctgaatc ttttgctcac
     7441 aaccttattc atggtgcccc ggcttgagct gatagatttt tgaaggacgg ccgattccta
     7501 caggacgttc accgatctca tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat
     7561 cgatcgtttt ataatcgatc cctaagaatt tagcaaatac ttttcgagct tccgtaatcg
     7621 tgaaatcttt acctaaaact tgtagaactt gcggctcgtg ttccatttta ttgactacac
     7681 ggttgaatgc tttcaagatg atttgactat ggtcaaaagc caaagtatct ttgccagaag
     7741 actctccagt gactaaatca agcgagatag ccacttctcc gctagataaa tggatcttgt
     7801 tactatgacg ttctagtgtg aaccagcgta cttccttggc atcatctccg gcgattagcg
     7861 gttcttctcc aataaaggct aagtagctga ctgtgacgac ccagcctcgc ggatctcgat
     7921 taggcgtact gaatgtatgc agttgctcaa tattttcttt tgaaataacc acaccagttt
     7981 cttcctttgt ttctcttaag acgctttctc cagttgactc atccttttgg acaaagccac
     8041 caggcaatgc ccatgaattt cgataaggat gcccttttct ttggatcaac aaaactttca
     8101 gctgatcttc ttctttgtta tagcacatca gtacgatatc gacagtcaaa gagggcttgt
     8161 catattctgg acgttcctgt ttttgatacc agttaagaaa ttcttcttca cttgctttat
     8221 gttcataata taatttttct tcttctttag aagcaaaacg gttcatctga ccacctccat
     8281 tgacagtaaa agtacttttt accttattgt agcagactct gaaaaaaaac caaggattta
     8341 cttgaaaaat ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata
     8401 aaaagaaaag cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc
     8461 cacaaataac gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac
     8521 tgttcaaatg cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata
     8581 ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca
     8641 tgattgttcc agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga
     8701 aataaaaagc cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct
     8761 attcatcctt ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca
     8821 ttttgaaaaa aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg
     8881 catcatgcga taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac
     8941 gccaatctat tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg
     9001 tgacgcaatt gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt
     9061 ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga
     9121 atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta
     9181 tgataaattc attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga
     9241 tcgtttatta gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc
     9301 tgacgaagaa ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa
     9361 cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata
     9421 cttcttccgc atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga
     9481 attcatccag ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt
     9541 agaggatctt gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc
     9601 gaaacatgtg gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata
     9661 tggctccgaa gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg
     9721 taaagaaatc gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt
     9781 gcctctacct aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc
     9841 taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc
     9901 actccgttat tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga
     9961 agattttgtt tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg
    10021 aacgattgct atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt
    10081 cactccattt gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga
    10141 tgcaatggaa aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg
    10201 tgcaaacaaa tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa
    10261 agctgaattg aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt
    10321 acttcagcca atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc
    10381 tgaaacaatg aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt
    10441 tgcaaaaggt acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca
    10501 agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga
    10561 aacagaactt gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga
    10621 actaaaagta gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca
    10681 attccgacta gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt
    10741 ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc
    10801 acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg
    10861 ctctttacaa gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa
    10921 attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca
    10981 attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa acagagaact
    11041 atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat
    11101 aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt
    11161 ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa
    11221 tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc
    11281 tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa
    11341 aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac
    11401 ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc
    11461 agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga
    11521 tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata
    11581 aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa
    11641 taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat
    11701 aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa
    11761 tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac
    11821 aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt
    11881 aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg
    11941 gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc
    12001 gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg
    12061 agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg
    12121 aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag
    12181 aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct
    12241 tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt
    12301 ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag
    12361 caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca
    12421 gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat
    12481 cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt
    12541 tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg
    12601 gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca
    12661 tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa
    12721 aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg
    12781 acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc
    12841 ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg
    12901 tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag
    12961 catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct
    13021 cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg
    13081 ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg
    13141 ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt
    13201 ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt
    13261 ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac
    13321 cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga
    13381 cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc
    13441 cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca
    13501 gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt
    13561 tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga
    13621 aaaggagaga taaacatgat atttgattct catacccatt taaatgctga acagttcaat
    13681 gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc
    13741 gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat
    13801 atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa
    13861 aataaattac aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg
    13921 gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt
    13981 gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac
    14041 acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc
    14101 agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc
    14161 ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc
    14221 gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag
    14281 cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt
    14341 ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc
    14401 gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag
    14461 aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga
    14521 agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga
    14581 tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa
    14641 acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga
    14701 gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc
    14761 ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg
    14821 ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa
    14881 cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca
    14941 agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta
    15001 gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt
    15061 ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata
    15121 cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg
    15181 caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga
    15241 tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa
    15301 cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt
    15361 actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac
    15421 tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag
    15481 cttatggttc attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg
    15541 taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa
    15601 gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag
    15661 cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag
    15721 atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac
    15781 gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa
    15841 atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta
    15901 ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg
    15961 cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta
    16021 agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt
    16081 acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt
    16141 cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt
    16201 ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa
    16261 gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc
    16321 gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc
    16381 ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg
    16441 cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct
    16501 ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga
    16561 ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt
    16621 ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag
    16681 aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc
    16741 aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag
    16801 ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg
    16861 tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa
    16921 cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc
    16981 ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc
    17041 taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg
    17101 cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca
    17161 tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca
    17221 agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct
    17281 acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa
    17341 cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca
    17401 tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata
    17461 aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt
    17521 tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga
    17581 tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca
    17641 cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact
    17701 tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa
    17761 gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca
    17821 cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca
    17881 aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct
    17941 ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct
    18001 tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa
    18061 acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca
    18121 ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt
    18181 tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt
    18241 catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa
    18301 tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa
    18361 ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg
    18421 aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg
    18481 actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg
    18541 ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt
    18601 tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt
    18661 ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta
    18721 agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat
    18781 ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt
    18841 agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat
    18901 aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt
    18961 gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg
    19021 ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca
    19081 tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc
    19141 acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt
    19201 agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg
    19261 cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat
    19321 ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg
    19381 agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc
    19441 tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat
    19501 gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat
    19561 atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa
    19621 aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc ccatcccaga
    19681 agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg taataaccat
    19741 ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga tccatcacta
    19801 atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa ctggtttttc
    19861 cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta tcgactgagc
    19921 tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga gcaatcgtca
    19981 acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta gtatctaatt
    20041 tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca tcattgattt
    20101 ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata
    20161 gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt tggatagagc
    20221 ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa cgcatcagca
    20281 aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg gtttgacctg
    20341 gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa
    20401 catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag gcatctggca
    20461 aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag
    20521 cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa aactgacttg
    20581 agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc ttcaatgcca
    20641 ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa cgagaagacg
    20701 ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga tcagcattta
    20761 gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg attttctggt
    20821 taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc tgtctatcga
    20881 aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt ggtgtagaac
    20941 aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac gaaagaatga
    21001 tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg gaaatattgt
    21061 ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca taataacgaa
    21121 gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg gataagtaag
    21181 ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt aaaacgatac
    21241 caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct tctccaatca
    21301 tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc ccattgatga
    21361 ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc
    21421 ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct cctgtgatgc
    21481 gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac cctgagcagc
    21541 aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc ggacacgttg
    21601 ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca aatccaagtc
    21661 tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg gcttggcaat
    21721 caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt tcccattttc
    21781 catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg cctgagcgat
    21841 tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt gaaggttttc
    21901 acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt gctgacggag
    21961 atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac cggaactgac
    22021 atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat
    22081 gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag aaagaccaga
    22141 atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag aagaaaaatc
    22201 gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca cctctttcac
    22261 ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag
    22321 tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt gttgcaagct
    22381 accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga cattcatgat
    22441 caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat aattgttcgc
    22501 tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg
    22561 gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat ctagtttttc
    22621 ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa tgattgagaa
    22681 aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa aagccatgac
    22741 cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca ttgctaaagc
    22801 tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg
    22861 attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac gtaactctgt
    22921 accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac aaatcagacc
    22981 aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc ggtcattcat
    23041 ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt cgaagacagc
    23101 ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt ttgcataacg
    23161 taataactga atcatatgat tcctcctatt atttattt
//
LOCUS       DASJUF010000041        23007 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJUF010000041 DASJUF010000000
VERSION     DASJUF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.041"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            474..725
                     /locus_tag="U4B62_001412"
     CDS             474..725
                     /locus_tag="U4B62_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141579.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="U4B62_001413"
     CDS             759..1037
                     /locus_tag="U4B62_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141580.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="U4B62_001414"
     CDS             1634..1891
                     /locus_tag="U4B62_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141581.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="U4B62_001415"
     CDS             2004..2615
                     /locus_tag="U4B62_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141582.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="U4B62_001416"
     CDS             2794..3351
                     /locus_tag="U4B62_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141583.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="U4B62_001417"
     CDS             complement(4050..4571)
                     /locus_tag="U4B62_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2141584.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="U4B62_001418"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="U4B62_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN2141585.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="U4B62_001419"
     CDS             8651..9538
                     /locus_tag="U4B62_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN2141586.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="U4B62_001420"
     CDS             9535..10704
                     /locus_tag="U4B62_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141587.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="U4B62_001421"
     CDS             10728..12083
                     /locus_tag="U4B62_001421"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN2141588.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="U4B62_001422"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="U4B62_001422"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN2141589.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="U4B62_001423"
     CDS             14369..14659
                     /locus_tag="U4B62_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141590.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="U4B62_001424"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="U4B62_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN2141591.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="U4B62_001425"
     CDS             15700..16359
                     /locus_tag="U4B62_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2141592.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16516..17448
                     /locus_tag="U4B62_001426"
     CDS             16516..17448
                     /locus_tag="U4B62_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141593.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17602..18891
                     /locus_tag="U4B62_001427"
     CDS             17602..18891
                     /locus_tag="U4B62_001427"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN2141594.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19138..20388
                     /gene="clpX"
                     /locus_tag="U4B62_001428"
     CDS             19138..20388
                     /gene="clpX"
                     /locus_tag="U4B62_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN2141595.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20471..21058
                     /locus_tag="U4B62_001429"
     CDS             20471..21058
                     /locus_tag="U4B62_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN2141596.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(21132..21287)
                     /locus_tag="U4B62_001430"
     CDS             complement(21132..21287)
                     /locus_tag="U4B62_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141597.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            21423..22751
                     /locus_tag="U4B62_001431"
     CDS             21423..22751
                     /locus_tag="U4B62_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141598.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            22762..22971
                     /locus_tag="U4B62_001432"
     CDS             22762..22971
                     /locus_tag="U4B62_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141599.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         7659 a         3915 c         4912 g         6521 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgattaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa attctttgtc aaatgtcctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg tgcttcagtt
    16441 atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg aatatgagca
    16501 agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa tttaatgcga
    16561 gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt atttcaacga
    16621 gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg tctttacgag
    16681 ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa
    16741 aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga ttttttatat
    16801 gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg ggtcattgaa
    16861 aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat caaggcactc
    16921 cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc gtctattgat
    16981 gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact tattgtatcg
    17041 ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca attgaacgaa
    17101 tgccatcaag ttttctattt gacgattgaa gaaaagttag tcaaagtcat accaaaagat
    17161 ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc agatcgttac
    17221 gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt gcaatctgca
    17281 attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt tcaactgact
    17341 gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa acaagatttt
    17401 ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat tttatcagtg
    17461 aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt ttacaatttg
    17521 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
    17581 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
    17641 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
    17701 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
    17761 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
    17821 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
    17881 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
    17941 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
    18001 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
    18061 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
    18121 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
    18181 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
    18241 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
    18301 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
    18361 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
    18421 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
    18481 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
    18541 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
    18601 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
    18661 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
    18721 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
    18781 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
    18841 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
    18901 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
    18961 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
    19021 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
    19081 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
    19141 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
    19201 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
    19261 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
    19321 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
    19381 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
    19441 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
    19501 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
    19561 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
    19621 cttaaattgc ttcaatctgc tgattacaat gtggaacttg cagaaaaagg gatcatctat
    19681 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
    19741 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagtgtg
    19801 ccacctcagg gcggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
    19861 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
    19921 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
    19981 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
    20041 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
    20101 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
    20161 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
    20221 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
    20281 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
    20341 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
    20401 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
    20461 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
    20521 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
    20581 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
    20641 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
    20701 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
    20761 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
    20821 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
    20881 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
    20941 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
    21001 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
    21061 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagtcttttt
    21121 ttgagcttaa cttacttttg tttcttgtct tttttctttt cattttgtgg ctgtggtttt
    21181 agaaatcgat cgatctcttc ttttggtgca actgtttttt ccggatcgac agcaaatgat
    21241 tcgaaaagtt tatctaaatt atctgtacgt tcaaatttca accccattgt aaacacctct
    21301 ttgtttcttt ttacttagtc tatcatgtaa tcgtccaaat tggtttctaa gacataaagt
    21361 tttagttttt ctttatcatt ggttataatt aaattataaa aagatttgaa agaggtgaga
    21421 aaatgattgg cgaagataaa ttattgatga ctatccagct cacagactta aatgaagaag
    21481 aaaaaaaaca ggaaataagc gattgggcta ttttgacacg tctgttgatc cagccaacat
    21541 ttatccgctc tttcacacaa caagcggatc cgtatgatct tcgaaagctt caagaaaaaa
    21601 taatgctagc atcggtaaga gacgaaagtt ggttagttgt aggaaatgac gagaacgaat
    21661 gtagttttcg cttggaagat aatcaattat tgataaaaaa tgtattgtct atctctgttt
    21721 tcaaagaaaa gcaatttttg attcgagact tcattcagaa aaaaatgatt gctcatgggg
    21781 tgttcgccta tatgcgagct tatagcgagt ttatctatca caatacaaaa cagatctctc
    21841 aacggctatt gtttgagaaa aaggatgaaa tcgaacattt acctaaaatg aaacaacaaa
    21901 acggggaagt agtagtcgat tgcaaccaat ttccaggata cgatcttttt taccaaggtt
    21961 tatgtttcac ttcgtgttgg gaaatgtatt atagtcgcta ttatcatcaa atcatcccta
    22021 aaccgatttt tctcgacatc caacaagtag aaaaagtgaa ggaattagaa aacgaagtca
    22081 tatgtatcca gctttatcga gaccccttta actggcaaaa gccaaacaat caaatgtttc
    22141 aatcctattt tagggaccag cttgggtttg atcatcttgc ttgggacaat ggtgtcggtt
    22201 tattgaaacc gccattcgtt gaatacattt acactgacca tacgatccaa tctgtccagt
    22261 atcagaatgc acaaatgcaa cctgttccta aaaagaatgc ttcatttttt gtaacaaaaa
    22321 gttatgatat ccaacaagga gattataaag aaaggcgtgt tcgcggaaca ctaaatgctc
    22381 aggcgtattt tccttgggtt gacgagaatc gatcacgcat gatgtgctat aaagttatcg
    22441 atccgactgt tgctttggat aatggaatcg aagcttattg ttattatatt agggaatatc
    22501 tagaagtaga ggtaacagat gaaaaatatc aagagtatct tttatcctta agaatctatg
    22561 ttccttcaga atccctgtca gaattaccgt taaaggaaat caaacataga ctctctgatg
    22621 ttaccttcaa acgaataaag aaaaagcggc gaagtatcca attcgatgtc aagaaaggtg
    22681 agcagcatct gcgtgtgcaa tttttagatt accgagaatt agaacaattg attactttac
    22741 aaaaaatatg aggtgaagaa aatggataaa caactctatt atcgaataat ggttccggca
    22801 tcattattat ccgtctgtct cttttttgtt cctaattttc ctattcgatc agtcatcggt
    22861 tttttggtca tgtttggcgg gatgggactt tattattatc ttcttcataa ggaaagaaaa
    22921 cctgcttctg tttatatgaa aaaaacaaga agcgaaaata aaaaacgata aaacggcttg
    22981 atgatagaca aaaaaataaa aaaccag
//
LOCUS       DASJUF010000042        22895 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJUF010000042 DASJUF010000000
VERSION     DASJUF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.042"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(120..1703)
                     /locus_tag="U4B62_001433"
     CDS             complement(120..1703)
                     /locus_tag="U4B62_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141600.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            1825..2409
                     /locus_tag="U4B62_001434"
     CDS             1825..2409
                     /locus_tag="U4B62_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141601.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2420..2665
                     /locus_tag="U4B62_001435"
     CDS             2420..2665
                     /locus_tag="U4B62_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141602.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2671..3393
                     /locus_tag="U4B62_001436"
     CDS             2671..3393
                     /locus_tag="U4B62_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141603.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3524..4267
                     /locus_tag="U4B62_001437"
     CDS             3524..4267
                     /locus_tag="U4B62_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141604.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4273..4638
                     /locus_tag="U4B62_001438"
     CDS             4273..4638
                     /locus_tag="U4B62_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141605.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4654..5184
                     /locus_tag="U4B62_001439"
     CDS             4654..5184
                     /locus_tag="U4B62_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141606.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5346..5654
                     /locus_tag="U4B62_001440"
     CDS             5346..5654
                     /locus_tag="U4B62_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141607.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            5650..6069
                     /locus_tag="U4B62_001441"
                     /pseudo
     CDS             5650..6069
                     /locus_tag="U4B62_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6206..7006
                     /locus_tag="U4B62_001442"
     CDS             6206..7006
                     /locus_tag="U4B62_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141608.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            7043..7225
                     /locus_tag="U4B62_001443"
     CDS             7043..7225
                     /locus_tag="U4B62_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141609.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7336..7653
                     /locus_tag="U4B62_001444"
     CDS             7336..7653
                     /locus_tag="U4B62_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141610.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7656..7888
                     /locus_tag="U4B62_001445"
                     /pseudo
     CDS             7656..7888
                     /locus_tag="U4B62_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8003..8482)
                     /locus_tag="U4B62_001446"
     CDS             complement(8003..8482)
                     /locus_tag="U4B62_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141611.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8602..9939
                     /locus_tag="U4B62_001447"
     CDS             8602..9939
                     /locus_tag="U4B62_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2141612.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            9962..10489
                     /locus_tag="U4B62_001448"
     CDS             9962..10489
                     /locus_tag="U4B62_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141613.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10476..11039
                     /locus_tag="U4B62_001449"
     CDS             10476..11039
                     /locus_tag="U4B62_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141614.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11461..12198
                     /locus_tag="U4B62_001450"
     CDS             11461..12198
                     /locus_tag="U4B62_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141615.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(12215..12631)
                     /locus_tag="U4B62_001451"
     CDS             complement(12215..12631)
                     /locus_tag="U4B62_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141616.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            13024..14271
                     /locus_tag="U4B62_001452"
     CDS             13024..14271
                     /locus_tag="U4B62_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2141617.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            14539..16929
                     /locus_tag="U4B62_001453"
     CDS             14539..16929
                     /locus_tag="U4B62_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HEN2141618.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            16978..18813
                     /locus_tag="U4B62_001454"
     CDS             16978..18813
                     /locus_tag="U4B62_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2141619.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            18882..19745
                     /locus_tag="U4B62_001455"
     CDS             18882..19745
                     /locus_tag="U4B62_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2141620.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            19735..20844
                     /locus_tag="U4B62_001456"
     CDS             19735..20844
                     /locus_tag="U4B62_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2141621.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            21183..21983
                     /locus_tag="U4B62_001457"
     CDS             21183..21983
                     /locus_tag="U4B62_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141622.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            22071..22877
                     /locus_tag="U4B62_001458"
     CDS             22071..22877
                     /locus_tag="U4B62_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141623.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
BASE COUNT         8504 a         3166 c         4462 g         6763 t
ORIGIN      
        1 gaaatatata aatcaagcga cagtatattt attattaaat gctattgtga gaatataact
       61 aattagttta atttgaaagt aaatatacgc tataaaagtg catcttgtgt tataaaaaat
      121 tattttattt tgtgaacata atttaacaat tctttctcgg aattgatttc ttttcttaaa
      181 tagtcttcag acttattaat aatattatct attatgtcta gatacatatc acttttcgat
      241 tcagctaatt catacagttt ttttaattct ttatccttta attgaaaacc ctgatccata
      301 gtcactgcat ttggatatag aagcccacta taatgtttac gtaaattcac ttggaatttc
      361 tgattataaa agtctaacag attagaaata aaaggcggaa aattactgtt tttgtaaaat
      421 tgattatttc catttaggtt gtatagtaat ttgttaaatt gctcctgatt taatttattg
      481 taaatccatg gtaatacttc catatcctta tctgctaacc tgaatgaact agcatcggta
      541 agatgttcga tcataaaatc tatgaacatc tctttttcac caaaatataa atattgatta
      601 tatattgttt ctaatgttga agatggtagc gaagaaactc tcattttttg ctggagtata
      661 ggatcaacta gactatttaa gtgattttgt aattcttcat tgttgtttcc tagaactgca
      721 agggaacgta aacggtagta atattctatt tctgttagta cttgttgttt cggtttaaaa
      781 tcatttattg atatttgtaa attttgaatt gtctcgtctg aaaaatcatg ttgtaattct
      841 ggatgggtag ctataaaagt aagtaaaaag aacccttttg tatacgtgat ctgttgattt
      901 tcaccaagtg atttattaat ttcctctctt gattcagctg tgaacctata ctttgcaatt
      961 tcttttttta gaatatttaa atagaattct ttattatcaa aaattagggc attaagtagt
     1021 ctgaaagtac tttccctatc tctatctgct tcctcatttc cataagataa ttgaaaaaca
     1081 aacttaaagg ctgtcttaca taggttttct ttggcttttt cgcttagttt atttagatag
     1141 ctatttttta gtgcagagtg aaattcttca ggtgtaacag acatagcatg tatgcgactt
     1201 gcatattgta taatttcgat cgttactttt tttcctaata ttggattttt taggaatata
     1261 atttcatagg acttccgtat aacatcctga caagtttctt ttgttatatc tcttaattcc
     1321 caagtattac cgttgtaagt gactacagga tgagctgaat aatttctttg atccttaata
     1381 tattttaaat cctcatattc atagctatca attagctcag ttgaactata agctttttct
     1441 agtagttcca tttcccattt tggagagttt ggatcacttt ttcttagtct agctatatct
     1501 tctaaaattt tcttagaagt attatcctcg taaatatttg ctaaatcaga taatttatca
     1561 attaaatcta aaataagcgc actgtaattg acagctattg cagaacgata atttccctga
     1621 taaaaactac ttctaacttc ttctaataat ggaataattc taggattttt aacttttttt
     1681 atttcatcat ctaatataaa catataaatc tccttttttt atttgtatga caagtttacc
     1741 ataaaaaaaa attaaaaagc gagacatatt tatgttgttg tgttatcata ataacataaa
     1801 tatgtagagt gagatgatga aaaaatggat ttgaaacaat cgttttacat ctggttatat
     1861 cctgataatc ctatgtcaat aatggctgat atttttgtag ttagcatctt attgtattta
     1921 ttttatctaa ttttctttca caatttaatc gggtacatga ttcttgattg gaaaggaatg
     1981 ttcgatatag aaagaaaacc atttgctaac aatcaaacct ataccgtctt cgaaagtgga
     2041 gattgggaag aaaatgaagt tgatgtgatc catgaaattg gattagcttt atcaggaata
     2101 caagatgctg atgttttggt atcaatgaca acggatactg gatacaacgc ttatgaggta
     2161 ttttatctta tgtattttat atggcgtgat ggtcaagaaa ttatcaatgg tcgtgctgaa
     2221 aaaacaatca attctaatac tgaaatttct ttttgtatgg gtgaagggga aatgattgta
     2281 tataaagatg ggtcagcaaa cattaaaaca gcctttggtg atattgaaac aagtattaag
     2341 gaacttaggg aatatgtgca gaacccatat aattttcaat atttagcagg aagaataaat
     2401 ttagtttagg agtaaaaata tgaaaaaaat tataaatcaa ccaacaaatc acttgataat
     2461 tgaaggtgtt attttcgtaa caatgtattt tactgtagga aatggcatct caaatacaat
     2521 cattcaaaaa aatccacaat acctattatt tagtctttat gcttggattg tacaaatggt
     2581 ttgggtttcc atattgatgc tgttgtttta ttggatagta cgggttattc ttttattgtt
     2641 aaacaaaaga aaaaaggata ggtgaataaa atgaaatcaa ctaaattgaa ggttgtattg
     2701 ttatcgacag cagcattaat aacccttaca gcatgtggta caactaatag tgctaataat
     2761 aacaaagaca caaagtctag tagtacgatg gttactaaaa acagcactac atcggcatca
     2821 acaacaatgt cttcaatgac tgaaaataat gctgaaaagt atcaaaaata cgaagacatt
     2881 aataaaggtc aaaagccgga tgaagtttat aacaacttta ttaatacagt aaaaaatgat
     2941 gaaaattatg ggaatgatcc attgcaaaat aaaaatctga ctatcgttct ttacaaacct
     3001 gattgtccag attgtcaaaa agcagaatca aaaatcatgc aagaaataaa agaaggattt
     3061 agttatgctc caacattaat gcatgatatt accataaata aaccagctta cgagtattac
     3121 gcagttgctg tagatattac tgatggtgta cctgagtggg tacaaaacat tcctggtctg
     3181 gctgatgaga acaatcaata tcatacgcca acagttgcag tagtaacacc aaagaaagtt
     3241 aacggcacac tatactggga tgtaatggga atgacaacaa atacaactga tgaaatgatt
     3301 gaaagtacaa ctaaatatta tatggatttg ggagcatata aattatatca acaatcagag
     3361 cctgttatgg acgatcaatc caaagttaaa tagatcttta gggcgtaagc cctatttttt
     3421 taaaattaag tgataacaaa taaatgattt ctgaaacaaa cgttcttact caactactcg
     3481 aaacacacaa gctaattaac tagattataa aggagaatgc gtaatgaaag aaatcagtgc
     3541 aaatgaagct catattccgc aaaagaatca ggttggtata tataaggtgc ttcatggtga
     3601 aggaatttat aaaatacagt tacttaccgg ttacaataat ggtgcaaatg ggaagtggtt
     3661 aaaggcaaca agtattcgtg aacttagaag tgaagtagaa aaaattaggc ttggtctcac
     3721 aagtgaagaa atagctaaac tgaagggaaa taattctttt gtaattgaaa aagaaaagga
     3781 aaaacatcta ctttatcgtg agctgaaaat tgaagaatac tatgaaattg atttacctga
     3841 aaaattaaga attattgatc aacgttcaga agaaaaatgg aaaactgaaa tatatccaag
     3901 cttaaatgac tacaagacat caggaaagcc catgtataac tacacaggct ttttaaccta
     3961 cacacaatac aaatatgcct ttcgcaaatt aaaaggcatt aaacaagctg aaattgccct
     4021 gtatgagatg aaaaaagtgg atagattgaa attgattcaa gatttagtgc aaaatacatg
     4081 gaatcaagcg attgtcgagc tagaaaaaga aaatgggaaa attttaacta ataaatatct
     4141 gatcgtaaat gaaattgaag aattaattgg tttattttca tgtgaaaaag gtgaagtctt
     4201 cttgaagtca tcaccaacct attcaatttt ggacagtccg tatcagttta tacaggcaag
     4261 gagatgagtt agatgtctag cttgaaagat gactatatcc aagcaaaaca agagtatgaa
     4321 aaaatacaga tgttgaaatc tgagaaagtc gaattatttt taacacaaaa attagcagag
     4381 gatcatattg ctaacttaga tgctaaaact ttagaggaat atgatcggca gtttaatcag
     4441 atacatcaca atatcatttt gcttgttgag caggcaaagc gaaggtttga aaaagcggaa
     4501 tatgatctat tagatcaagg aattgtatgc ttggataagg gattgcgaag atattatggt
     4561 gcagagttaa aaaagcaaaa tgtagataga aatgcactaa aacatgaaat cttagaattg
     4621 ataatgcaac gaatttgaag ggatagaaag caaatgtcac aaaacattag tgctgtaaaa
     4681 caattaatag aagaagattt agagaccaca gttcagagat tagcggaacg aatccatttt
     4741 gcaaattatc aaaccatctc taactggtca agcaggaaca gaaatccgga agaaattcca
     4801 atgaaatttt tttatcgaat cggaaaagaa tatggtgttg atccgataaa aattttcgag
     4861 aagaccatga tttattacta tcatggaaca cctaacgaag tgttagaaca tcttttggaa
     4921 gaggcaaaag aaaaatcatc aatattagag ctatcgcaaa atgacaaagt tattcaagtg
     4981 aaatgcgttg atccaacagg aaagattatt gtcggttttg atattgaaag aaaatccaaa
     5041 atagatttta ttacacaatt aaaaaagata aataatttag atattctaac gcttagacca
     5101 gatcatgaac agttgtttat gagttttaaa aaacaattga aagaaataca aaaaagatat
     5161 aacaaggtgt ttcctaatgc gtgaaaagct caaagaaatc agagtttttt ggataattaa
     5221 caaagaatgt atcatcttat atactcttgg atttcttagc atgatcttgt tttggtggtt
     5281 atatttaaaa cagtaattgg agagggtcaa gaggattttt atcaagataa ggatgtggaa
     5341 actgcatgtg gaataataga ttaaaaacag gattgttatt aatagttatt tcctgcgcta
     5401 tgatgattgg tatgaggata caaagggaac aatcctattt tgaagtttct gctaacaacg
     5461 taatagaaaa atgctattac ggtcaacatt attggagtga ggaagtcaga gaaaatatag
     5521 atcgtgaata tgttcaaaga attgtttggg atgcatattc tatcaaagat tatcctaaaa
     5581 gtttaacgag tagattattt tacagtgaaa aagataatca aaaactctca gatttgatga
     5641 tgaaaaaagt atgactatta ttacgaaaga gaaacagagt gctatattcg aggggaaaaa
     5701 gcctacaaca aaagtgacta ttggggtgac aaggcgaagc gattaactgc aaattttaga
     5761 gtgtatgaat cagagtttat aaacttgacc tttcttaata gtgtttttat tgattatgta
     5821 attactacga aacgcattgg aagttattca agtacctacg catccttagt tccaatgtta
     5881 aactcgatga gccgtttctt aaaagaaaga gaaaagaaag agctacagta tatcaaagaa
     5941 atcatgccgg attttgaaat gcacgaatgg caaattgctt tatcagattg gaaattggcg
     6001 aataaggttc gtagaataac accttttcaa gccaagcgct ttttgagagc tatgaaattt
     6061 gtgcaataaa aaaagccacc agacaagttg atggagacta acacaattac caacagttgt
     6121 aattgtgtta gcttacaaat taattatggt gtttagtcaa tcccaaattg tgaaatggca
     6181 ccaataattg gaaaggaaaa atgaaatgtc tgaagattta cgagagctga agttacgaaa
     6241 gaagcagtat gatcagtatt tttccggttt gttgaataaa catattgaag aaggacttga
     6301 tcatgatttt ggaaaagatg aatattgttt atattgcgaa cgttacaatg aaaaaatgtc
     6361 tgaatatcta cagctgaaaa aagagataca gaaattagag aaaaaagtac atgcttggaa
     6421 gtataaagat tccatgtctg gttatgttga atctcaaaat aagccaagaa aaaagaagca
     6481 agcgtatgtt ctaaagatgg taaatgggga agagttaatt gtattagcgt acagtgaaga
     6541 agaggttcga gaactcgttg aaacacctgt caggaaaaag gttgaaacaa taaatgagat
     6601 agacgacagc caatacaata gcatctatat tgaaacaagt catggtggat ataagaaatt
     6661 agcaactttc cttagaagtt ctaaagttgg gataatttct aaaaaagaca taagtgctgt
     6721 taccgatagt ttttgtactt tatctgttcc taaagcttat ttccaatacg atagtaaaag
     6781 atttgaacgc ttagagaaat tctgtagacc gtatactgta ttgaaaacta aaaaatttaa
     6841 taaaactaaa agagtttcta tacagttacc gactggtcaa atctcattag gtgaaaaaga
     6901 ctatctgata aaagtaggaa aagagttaat tataaaagta ccggaacact tatttaaaga
     6961 aaattttgag atacgtgaaa ccttggtatg gaaagaagaa aaatagtttg gaagacatag
     7021 aatggtataa ggggaaattg aaatggcagt aaataaaata ttgcaggtac taatcataga
     7081 cgaatttggc ggaggagaat ggtttgatgt gcagtccata gactttgaag aagaagtcgt
     7141 agttatagaa aatatttacg gcgaagtata ttcatattcg cttgaaggtg agaatattga
     7201 ggatttacgg ttgattggtg agtagaaaaa aacagaaata tcaaaatgga gataaaaatt
     7261 aatagcatgt aaagatagtc atgcttgggt taaaaatgtt aaaattcaaa aaaatgagtc
     7321 agaagggata aaagtatgaa aagagaggaa tacaaacaaa gattaaacga actgcttgaa
     7381 gaggacgaaa cattaactca tggttcgcct gatgaaattc tatacatgat tgataatatg
     7441 gtgatctttg gtggatatga acttggtaat aggtcagttg atcataatat tttagaattt
     7501 gatgatgtat catgggaaga aattcttgat tggggaattt tggcggtacc tgaaactaaa
     7561 acctatatta gtgacactat ggttcctttt tttgaggaat tggattataa aagacttccc
     7621 aaaaatgaaa atcatatact gggaggaaac taacaatgtt aagtgactta tttattttac
     7681 tgatctatct aggtgtaagt gaaattattg cgtatttttt gtttgtcatt gctaattatg
     7741 gtatttccag tgcaatcgtt caaatgttta gattaccagt aagtgaacta aaaaaatgat
     7801 gagattatca gcatatcttt taatgttttt gaatgtggtc acgttaatct ttgtagtaat
     7861 cggtgtgact ttcgatttaa tggaatagtg ggaaatctat aatggaaaaa agagacacga
     7921 atgaacatat tttttgaatc aatattgttg atggtttttg cgatcagcgc ttaccagaaa
     7981 tctaaaaaag agtaatgatt gtttaatcat cactcttttt ttgtagggaa attgaaatat
     8041 gtggagcttg tattggttta gttaggattg cttggctata aggagctagt tctttatcaa
     8101 aaccatcaaa gtaggttttc agtagcgatt taactgatcg ctcatcttgt ttgatttgga
     8161 taatttcagc atccaaatcg gttgtgagat caggagcttt tgatgcgatt acctgatttt
     8221 ttttatcttc caactctctt aggctgttta acaagttgta aaaagaatca ttttgattgt
     8281 tcattccaac gtgagttgca attttaacag aagtttcgtc cgtattgtca tcaaaagaac
     8341 tcattggaat atcaagtagt aacgaggatg tttcttttac tgactttttc tttacagcat
     8401 tttttgtttt tccatctttt ttgctcttta ctcgagatgt ttttggagct agtttaggta
     8461 aatcacttgt tttatcagtc atttattagc ttttcctttc tattaattta tgttattata
     8521 ttatcataat aacataaaaa agtacacttt ttgggggcgt agttattgaa aaaatctaag
     8581 agaaagattg ccacattgct tatgttaatg gtgcaattta cagggattgc aatgagtgta
     8641 atcacaataa atccgcaagt gatacatgct gatgggatag ctacttatga tgcacaattt
     8701 aactgtacta ctgaaggtag tgggtcagga acagatagcg atggaaacga tactggaagt
     8761 ggcgctgtaa caggtgatgt agccaaaaat attgaagata tttacaaata catgaatgga
     8821 gaatatggct tatcaggtgc tagtattgca ggtattttag gtaactggaa acaagaatca
     8881 ggtattgatc ctcgagcagt agaagggtat tacggagatg gacagagaga agttgacttc
     8941 atcaaacaat ttactgatgg tctgatgggt attggctatg gtcaatggac agctgggcga
     9001 catacagcat tagttgattg gtctaagaaa aataatggcg gttcttggtg gactacagaa
     9061 gcccaattgg actttatgtt caaaggtgat gggggattcg taggaatatt aaaagattat
     9121 gctttaaatg cttctgatga tgtcgtagaa aatgcagtaa cttttcataa aaactgggaa
     9181 atttcagctg attccgaatc agctgtaata gctgtacgtg gtgaaaatgc aaaagaaatc
     9241 tggaagtaca tgaaaagcaa gggtatggac ggtgcaaaag atgaaagtaa gatcaataaa
     9301 atatccggag gttcaagtac cggagatgga tcgaataatt catcaagttc agcatcagga
     9361 acagtagaag atgcctgtgc aactacggat gacgctaatt ctggttctgg atcaattggg
     9421 aaagaaggaa aaattaacgg taaaaaaggt caagtaatag cgaatatgac ttatgaagaa
     9481 gctttgaaaa aatatgggaa agcgatcacg ctaccaaaat tcaaagaacc agattggtca
     9541 acttcacctt ttaacggaat atatcaaggt cagtgtacag agttgacgtg ggcttatatg
     9601 agccaattat acgatgggaa tcaaccgact actggaaatg gtggttatgt atgggaaagc
     9661 tacaaatcgg caggagcaaa aataacgaac aaaccaatcg taggttatgg gtttagtgct
     9721 tcggatggat ggttatgggc tgttggagat gcagggcata ctggtgtagt tatagcggtg
     9781 tttgaagatg gatcatttct atgtgctaac tttaatgttc caccaaccca agctccaaca
     9841 aggggagttt cggtaacgtt gattgatgga gtggacggat cagataatgt gcatttcttt
     9901 agcggtgtag gaaaagctaa atttgaaatc gaaaaataaa ttggaatgaa ggacgatata
     9961 gatgaatagt aaagtgaaag atacaattat catttctttg ctttctggta tgttattagc
    10021 actgcttgtt ggaatcctat tcttcaataa gcagaatcag gacatagaag ctgaaaaaca
    10081 cagtgtgcat attgaagata agctgacatt ctttaaagag gttaaaccaa atgaggtgga
    10141 tggattacgg aaagatttag aaaatgatat tcaagaaatg ttgaatggat cagttcaaaa
    10201 taagtttgca gatcaatcag gtgaagaatt tttgagaaaa ctatttattg taacaggtaa
    10261 accaccaatt actgataaga gtagtgagaa agaaaaaatc gagtactata agccttttac
    10321 ttttaagcta acaaactttg gaattaagac atctagtagt gggttgtatg aagcattgac
    10381 cgattttgaa gttcaataca atggaaaaaa agtgtatgat accacattaa ccatcacact
    10441 aaataatgat aagaaagtga ctgggggtgc tttttatgca aaatcatgaa atcactactg
    10501 ctaaaaaggc gatcggaata ctttccataa ttgtgatcct ttgttgtgtg gtactatttc
    10561 aaaagaaaaa tgcttatgat aataatgtca aagaattgga agaccttaaa agtaaacaaa
    10621 tcgaagtagt caagaataac cagaaagtga aagctatgca ggaagctcaa tacaaagaat
    10681 tgggaaatgc tgaaattaag gaacaaagta ggaaattctt taatttattt tacaactgga
    10741 attcatggga aaaatatacg aagaatatga aggaaataca aaaagaattt cctcagatag
    10801 taaagtctag tcctgttgat atttctggca cgatggtagg cactggtaat agtccaatat
    10861 ctagctatac tgctgattac tatacaacaa atcaaaaagg taaaatggtt gaattgatta
    10921 cacagacaag aaatactcca acaacagcaa caacaacaat ttggcgtgga gtaacaagca
    10981 taggagaaaa tcaattgttt tatttggagg atttagatag ctatgtaaga gcagagtaaa
    11041 ataaaaaaag agctaacggt accaacgtta gctctttgtg ctgacgatga taacaaaacc
    11101 taccgaaaaa ggattgtttc atcacaaaca caaacagaaa agaattgata aaaaacccga
    11161 acctcagaat ttttattaat caattcattt agattataat aaattgagct gaacattaca
    11221 atacgtatat gttgtaaaac gtaaaaaaag tcaaaagtaa atactttctt aaaaagtcaa
    11281 ataaaactat taaagacaat atatatggtt tatttgacat aagaaaatgg tctagatgat
    11341 aattgtaata tagaattgta gacttaaata aagcataaaa taccttttat gataaggtat
    11401 agctcttaat tttaagcagt tttagatata agattgaaag taaagtgggg gagaataata
    11461 atggcagatc ataaattaga aaagttatta gaagaaacgt ggaatccaaa agaatttagt
    11521 gaatttttta tggagaattt tgaaactgac ttagcagtga ttgttaaaga tgctcttaga
    11581 gagcaagggt atcctgaaac tgcaaactat ataaacatta attttacttt gtatactgaa
    11641 aataaaggaa cttgggattt ttgggctact ttagcaaata aggagttaag tgataaatca
    11701 gacacaggaa ttcgtaactt ttttgaatca aacagagatg attatatgta tgctaacaat
    11761 caaaataaat tgaactttag agtagagttt gatgaaacac ctgaagaatt tatagaaaga
    11821 caaccgccta aagaaaatgt agcaaaggta ttggaagatc gttggaattc agatgaaata
    11881 gtttcaacaa ttagcgaact aggcggacaa tatgaaccgc tagtagaagc ggttagagaa
    11941 gagctaagac tgaataagtt tcctgatgtt caaaatattg atgtttctca aattgaaata
    12001 aacgtcaaga tcacgaataa acttgattat ggtagttggg cagatatagc attagaaaaa
    12061 tacatctact caacgttaaa agagtttatc gaaaatagaa tggatatcat gtatctacag
    12121 catccacagt atttaaactt tggtgttgaa atagctacac ctcttgaaga atggaaaatg
    12181 gagcaagggc ttgactagcc tttgctccat ttttttattt tacaaatgaa tacagttcgt
    12241 cgtttttacc aagaatgatt gtttgcccaa ctttgagatt gtcaggtgat ccttcagcgg
    12301 tttgtaagga gaacgttcca ttgaacttat cggataccgt tataggtagc tttaattctg
    12361 atccttcctc atctttgaga attgctgttt ttcgttcatc aaaataaaca gtctttatta
    12421 aataattttt cgtagtgatc tcagcagatt tgtctttttt tgtagtagta ccagtttctt
    12481 tagaattaga ggtattttct acgactattc catctttggc atttgagctt gtgtttattt
    12541 gattgaatat tttcagcgac aagataacgc taagaatagt caatatagaa acaagtgtaa
    12601 ttaatactga taatttcctt tttttgttca ttttttctta acatcctttt ttgttttgat
    12661 tagttagata ttccttatct aacaagcctt tttaggctat taaatgaaga taaaccccaa
    12721 aaaaatattg caattatgac agtgcaatgg tactattatt atacaataaa ttttgcgaaa
    12781 ggggtaggtc taaatcgcaa acaaaaagaa attgaaccta gacaaataca acttgtatac
    12841 cgcaagggaa gcaagtttga ttttgggtaa aggtagaaat tatgttacaa acataatcaa
    12901 ctattatcca aatcgtttgc ctaaaggttc gttcaagaaa gtgggtgaca aaacctatat
    12961 tataacggaa gaaggaatag aagcattgcg atcacaaaca gaaaataaaa acaaaaagaa
    13021 ggaatgaata tgaagagaaa ttcgcttggt atggttttat tggcatcagc tgtacttgcg
    13081 gggttatcag taccagtaac ctcacatgct gatgaatgga ctgcaaacac accggatcaa
    13141 atccaaatta caacaggaca atcaagttat gcgttcaaaa aaggagatac tatttggtca
    13201 actgtagaga agcttaaaag cttaggttac aagactactg ttgctgagtt tgccgaattg
    13261 aacaatgttg atttggcaaa aggagaacaa cggactatcc cgattggacg aactttcaac
    13321 atcagtaaag atacattaac agttaaggaa gcagatggcg aagtagttag tcaaactgtt
    13381 caaaaagata aacctgatcc tattgaaaat acggagcaag agaacaaaaa atccgagact
    13441 acaaaagaat cggaaccaac agcaaacact tctgataaga cagaaacaag tgcttcaagt
    13501 gttgatggag caagttcatc taatacaaat aattctacaa gtgagaaaga agcatcacag
    13561 ggaacaagtg aaagtagcac tacggaagct gatactggaa catcaacaaa taactcacaa
    13621 tcaacatcaa atgatgcaaa tgaatcaacc agtacatctt ctagtgatca atcacaaaca
    13681 ggttcgagtg atgaaactga cagttctact tcaccaagta aacccgaaca accaactaat
    13741 gttgtgctag gcgttccatt tattagtcaa ggtaacacaa tgctttgcga aggaacatct
    13801 ttgctagaag cgttacatta taaaggtgtt gcaacaaatc aagatttaat gacttttgtt
    13861 aaatcaatgc cactatctcc tgacaataat ccaaataatg gtttttctgg tgaatggaga
    13921 cacaatgtgg atggaactta tcaaggtatg aatccggctc ctgtagtttc atggggtgtt
    13981 gctaatggcg gaaatgttgt agatatttct ggttcatcgg tacaaggatt aaaagatgag
    14041 attgtaaatg gaaatcctgt agtagcgtgg gtaacttatg cctttgaacc tgctcaacat
    14101 aaaccaatgt tttggggcga tgctatttgg aatcgacatg tagtagccgt tgatggattt
    14161 aaagacggtt tttaccatgt tgtcgatcct gtgtttgggg caagttggat taatgcagga
    14221 tcatttgaaa cagcatataa cgtatctcaa ttagcggttt cggtacgtta ataaaagata
    14281 tcgtttatat actaagtagt tatatagtct ttatttgtcc ttgcttaact acttagaaaa
    14341 cttgttgctc atggtgggta gaactaaaag gggtgatgct atgaatacta gagcgttgaa
    14401 acagcttgct ttcaacgaaa aaaatgatta ataggtgtgg tgattttaac ttaggaatgg
    14461 gcgcgaaggt cattaccaca ccgacatatc gtggctcatt ttcttcgcct aactggtata
    14521 aagaaggagt aacgagagat gaagcatgaa aaaacgataa ttttaaaatc aaaaataatt
    14581 tattgttttg cgactttagt aatgatgtta tctacgctta taatgccttt ttcagcaata
    14641 agagctgaag cagatgtcac aaaaccgaca agtgcaaaat ttatagaaac tggcgtaaaa
    14701 acagacggtt atattagggt taatatgccg aatcatccaa atgaatggat gatttcaagt
    14761 caatttaaag attctcatgg aaatattgga tattgtatgg attcagaatt gccatcccca
    14821 acaggaagtg gagccggaag cttgaagtac aagggagctg gcagtgatga gttctatcgt
    14881 atgtttaaag gtggttttcc atcaaaaaca gcaaaggaac tgggtgcagg aaatgataca
    14941 gaagcatggt atgcaacaca attagtttca tgggttttag ccggaaactt taaagtttca
    15001 cagattgtat ggtcacatcc aaaccatacc gccgcagaaa cagctcgtgt aaaaaaagca
    15061 tttgaaaaaa tttatgacta tgctaagaat ggaaaagata cgcccaacac tgagttttct
    15121 atcacagctt ctaagacagc tgacgaagga aaatatcaca ctttcacata taaaacagca
    15181 tcaaataaga ctggtaatgc gaagctcact tttacatcag caaaaccagc tggtatgaag
    15241 atttatgatg cagatggtaa agaaattact aacaacactg tgaaattaaa ttcaagtttt
    15301 actattaaag tacctgtgac tacaccaagt ggtactttgt catttaaagg tactgctaat
    15361 gttagcacaa ccaatccttt tacttttgat ggacgaggtg tgtatcaaga tgccgtagtt
    15421 atgatcacta cttcagaaac aaaggactct aaatctttga gtgctaaatg gacaagagct
    15481 gttggtcagg taacagttaa gaaagttgat caagatggaa atgctttaaa aggagcggag
    15541 tttactctaa ctgacaataa caatgctaaa tctgttaaga caaccggaga tgatgggtta
    15601 gctacgtttg atttgcaaca aggcttaaca tacagcttag aagaaacaaa agttcctgaa
    15661 ggttatcaag gttcatttaa aaaggatggt attactgtta ataccgatgg acaagaattt
    15721 agttacactg ctacgaacac aattaaaaaa ggtaaagtaa aagttaaaaa ggttgaccaa
    15781 gatggaaaac cattagctaa tgctgaattt accttgacgg atgattcatc ccaaacacaa
    15841 gcagaaaaaa cagatgaaaa cggacttgca agcttcgata ttgtagcaaa caagacctac
    15901 agccttgaag agaccggagt accaactggt tatacaggcg tattcaagca agaaggaatt
    15961 accgttaaag atgatggaca aacttttgaa tatacagcta caaacacttt gaaaaaaggt
    16021 ttagtaaaag ttcataaagt cgatcaagat ggaaaacctt tggcaaatgc agagttcact
    16081 ttaaccgatg aaaaaggtgc aacacaagta gaaaaaacag atgaaaatgg acttgcaagc
    16141 ttcaatattg tagcaaacaa gacgtatagc ctagaagaaa ccggagtacc agtaggttac
    16201 acaggtgcat ttaagcaaga gggtatcacc gttaaagacg atgggcaaac ttttgaatat
    16261 acagctacaa ataatttgaa aaaaggtgta gtaaaagttc ataaagtcga tcaagatgga
    16321 aaacctttag cagatgcaga attcacctta acggatgaaa aaggtgtaaa agaagttaaa
    16381 aagacggatg caactggtct ggctacgttc aacattattg cgaacaaaac ttacaactta
    16441 gaagaaacaa aagtaccaac aggttacaca ggcgcattca aacaagaagg aattaccgtc
    16501 aaagatgatg gacaagtctt tgaatataca gctaaaaaca ctttgaaaaa aggtgtgatc
    16561 aaagttcata aagtcgatga tcaagggaaa gcattagcaa acgcagaatt cactttgacg
    16621 gacgacaaag gtgtaaaagt tgtacaaaaa acggacagaa atggcttggc aacatttaat
    16681 attgttgcca acaaaacgta caaaatcgaa gaaacacaaa accctaatgg ttatacaggg
    16741 aaatggtcta aagaaggcat caccgttgct gacgatggac aaacctttga atatacagta
    16801 attaatcacc ttgtacctga aacagtaaga acaggcggaa tcggtacatc agccttcctt
    16861 cttgtatttc taattgcagg attgagtatg gtgctactgt attatcgaga acagcacaaa
    16921 gcaaaataaa aaagtgttaa tggaaaagaa gtaaatatga aaaaagaagg ctgacgtatg
    16981 aaatcaaaaa aaattttttc aaatttaatg ctatttggta ctttgctagg agtgggattg
    17041 ggcgcaagct caatctcttc tagccttgga ctaggggggc aagtagcata tgcacaacaa
    17101 aactataatt atcaaggaac tggaacgatt catatccata aagtaacaaa tactaatggg
    17161 gatgggaacc ctgtctcaga tattgataac aaagggaaag aaatagcttt accaaatggt
    17221 gtaaaacctc ttgaaggcgt tgagttctct tatgtgaaac ttactaaaga tgaagctaaa
    17281 tccttgggat ggacggatag tgatactaca aaaggtcaac atattgatga tgctaaagca
    17341 cgattattac taaacaaaaa atacagtgaa gtaagtagaa tgaagactga ttcacaaggg
    17401 aatatagaca tacctataca agtaaataca gacgatttaa cagataactt gtatttgatt
    17461 gttgaaacag gaacagtcaa aggtgtagtt gaagaagcgt atcctatgat tgttaacgta
    17521 cctgaactaa acggatcaga tgatcaatac tttaattatg atgtacatgt ctatcctaaa
    17581 aactatttcc aattaaactc agcagagctt gataaaaaag acgaagatgg aaaggctctt
    17641 gccggaactg tttttgaatt gcgaaaagat gatggatctg atcaggttgt tggaacttat
    17701 aaaacagaca gcacaggtaa attaactgtg gatggtttgg aattaggtga ctattatttc
    17761 ctagaaaccg atttgggaac agatgctaat agaacaacct atttactaga tggtaaaaaa
    17821 ttcaaattta gcgttaaacg tggggagact gctaaagtag ttcttgatgt taccaactac
    17881 aaacgacctg taattactaa ggtaattgat aaagaaaccg ttgatactgg cgaagatgtt
    17941 caatatacat tgaaaccatc aattcctggt aatattggta attacgaaaa atatgttgtc
    18001 aaagatgttc taggtgcaga gttaacttat gattcatcag aagtgatcga aggaatgact
    18061 ttagatactg attacagtat cgaacgtgac ggacaaacga caaactatgt ctttactgaa
    18121 tcaggtatgg ataagcttca gaaacaattt gatgcagggg atcgcttctt tacaattttg
    18181 atgaacgctc atactaatag taaagctaaa atggatacac cacttaagaa tgatgtttca
    18241 acgatctaca acaatggata tgaacccgaa gacgaaacac cagttgatca cgcttataca
    18301 atcattggtg atcaccaatt cattaaaaca gacggagata caagcaaagc attgagtgga
    18361 gcaaaatttg tagtgaagaa caaaattgat ccgaataaag ttacttacct aaaacaagat
    18421 ccaacaacga aagctgttac ttggattgca gataaatcta aagctactga attcgtttcc
    18481 ggatcagacg gtaagttcca aattgaaggt ttgcaatata cctatgatgt agattttgat
    18541 aaacaaggta atccaacaat cacagataaa aaaatcaatg attatgctct tgaagaagtt
    18601 gttgctcctt caggatatac accaactaga aacgatgttc catttgtggt atcgaatgat
    18661 gatgcttcta gcgaagtaac agacatcaag aaccttaaaa cacctgaaac agttcgtaca
    18721 ggtggaattg gaacagtagt atttctagtg gttgcaggtg tggcaggaac agggctatat
    18781 gttgtctaca aacgagaaca acacaaagct taatgaaata aaattcccta gagtgttttt
    18841 ggctctaggg tttcttttta aaaagtgtgt gaaaagtagg tatgagcatg aaaaagaaga
    18901 atatcaagtt gatcatctta aaaattggga tgttgctatg tttaggagta ggtctatatc
    18961 ctgtaattac tcaggcttat acctactacc aaactcaaca aacactaaac tcctataagg
    19021 aaaaatatga tgaagcttct gaaaaggaga atgaagaagt cttcaagaga tattcagaag
    19081 ttaataggaa acgaaaagac acagaacaaa cctcagtcac gattcctgaa gataataaca
    19141 atattccgtc aaatggaaat cttcagaagg ataaattagg tgaattagtg ggtgaaattc
    19201 ttataccaaa gattaatgtg gatttgccaa tttatgatgg tgtaaacgat ttccaactat
    19261 cagcaggagc aggaatatta accggaactg attatccaac tggtgggatt ggaaaccatt
    19321 ctgttatatc agctcattca ggcattccta ataaaaaata ttttacagat ttagagttgc
    19381 tcaaacttgg tgataaattt tttattgata tttcagggaa aaaacttgct tatcaagtag
    19441 atcggataaa aacaattcta ccaaccgaat ttgaggattt gcttcctgtg gcaggaaaag
    19501 attatgtaac acttatgaca tgtacacctg ttggagataa ttctcatagg ttactcgtta
    19561 gaggggttag cataccttac caaaaaagta ttgttgagaa agaaaagaag aaagaagatt
    19621 taaaggtaaa ggcgcagatg aactggtatc ctatcgtagt tgtaggggtt ttattaataa
    19681 tagctgtgat agtcattatt aaaaaaatag tagcaaagag acgaggaatg aaaaatgaag
    19741 aataaaatac tacttttttt gactcttatt tgcactttgg ttttaacaat ttatcctgca
    19801 aatcagctct atcatcaatt caaaacaaaa gagaatgtgg cggaaatcca agagaaaatt
    19861 gagaaaaaac aaactccttc aaaagaggaa gtggcaaaaa aagtcgaaca aataaaatcg
    19921 aacagcggta accaaggatt agacaatact cagaaaacag ataaaggagt agacaatact
    19981 caggaaacag acggtggatc atttgatatt cgtgaagctg aaggaaaggt caatccatac
    20041 acaggagatg ttttttcaaa aaatcaaacg aaagattttg aaccatctac ctattcagga
    20101 gcggttggtt atgtctatat tccaaaaata aatgtgctag agcttttgtt tctaggcgct
    20161 tcacaggagc atttatatag aggtgtggca caaatgagtg ctactagtct acctttatca
    20221 ggtaaaggtc atcaaacagt aatagcaggt catcgtggcg gttatacgtc accacaattt
    20281 ctttatgttg atcaattagt agccggagac cacatttata tagattatct tggtgaaaaa
    20341 gcagtttata cggttttccg aacagacgtt attagaaatt acggtcagaa ttcaacactg
    20401 actgatattc ctgatttaga tatgttaaca ctttatactt gtcatccata tcctcagaac
    20461 tacgatcgtt tattagttag aagtctaaga gatgaaaaag caaaatacat cacgccacaa
    20521 gtaacagttg gcaataccgc atacgctgat gccattgata gcgagtccgt ttcagataag
    20581 acagaaactg ttacatcttc taatgattta tttgaggatg ttgatcgtgc caacaaggaa
    20641 ttagatggaa ataatgatct taatattccg gaacaaggta gcgtagagat gcaagacaga
    20701 acgtttgctg aaaaggcaag ttcaatattt acaggaatta ccaataaaca tcagaaaatg
    20761 cttttagtca tttggactgt aataggagca ttagcaagtc taaaggttct tattctttta
    20821 atcaaagagt ttcgaaaagc ataatgaaga ttataaaaat tgatagagta cagtaaaaag
    20881 ggattgatac tattattgat ctctttttta ttttgaatag agctagattc acttttaaat
    20941 aaaatcaaaa ctgatcttaa ttctaaagaa aaagtcaaga aaaaaataca acaaattttt
    21001 ttatcagaat atttatcaga atattattag aaaagataaa ctcaaaagct taatttaata
    21061 gggttattgg ttgtatttag aaataatgta tggaaaagct ataaatcaat agataagatg
    21121 tagaaaatct tatttgtgaa tgaaaaacaa gtcatcgtaa aatattaata ggaggtgtag
    21181 taatggtggg aatatcaaaa acagctttag actcattagc taaactattt agtagcaggt
    21241 ttgatggtgc agaatttgtt gaatatctaa gccaaaacga aagtggaaaa actctgatcg
    21301 aaacaatgaa aaaagactat ttgaatgaaa atgactttcc gacatggacg aatattgaag
    21361 atgctacatg ggatgtagta tgtacgatat attctccttg ggtaacgatt gctgattttg
    21421 ttttagataa aattggaggg gatacatacc ctgatgccaa atctcttgct acaagtgact
    21481 ttaaaacgtt ctatggttat ctaataggtc taataggcaa tggaatagat ttgaaagtta
    21541 cgccaaaaca aacacctgaa gaaattgaaa aagaaaaagg agaggttcaa atggcttatg
    21601 caaatgacag agaaaaagta aaattattgg aaggtaaatt atctgaagaa gaacttggaa
    21661 aaatggtgca agaaatcaat gaagacagca actattttcc tgaattgaag gtatatcaaa
    21721 atgacgaaga tttctttaat gaaatgttta gtaatagtcc atgggaagca gtgagtaaaa
    21781 caaatttagg agaatggaac tttcaagatg atctagttgc atttagcaat gatgggtatt
    21841 tacgatcata ttcagaaagt gagtatagag ctgaattaaa aacgtatgaa acggatattc
    21901 tgatgagcta tttaaatcta ttagagagtg gtcagggaga gttaagagat aaagttgaag
    21961 aactattaaa agaagagcta tagggattat ttcaacataa acacaagcag aaaactacta
    22021 aacaaaacgt accatgcaaa aagtcggtta aaccggcttt ttgctctatt atgagaggag
    22081 atcaaaccat gagtgcaaca accgatatgg ctttagaagc agtaataaaa gataatacag
    22141 gaaatgtcga aaacgtagaa gactttagcg atattgcaat caatctttca gcatggattt
    22201 cagctgtagg agaagaaaat atcgaatttg agatcaagaa tatggatgac ttaaaagcaa
    22261 aatttccttt tatcgcatcg aaactaagtc aagttgccga ggataaatcg tggaatgaat
    22321 tagttagttt ttccgaaaaa atttatgata ttacgcaaga agatgagtgt gtttcatggg
    22381 aaatgaataa tcaatacaag aaaattgacg ctttcgtcaa atcacgtata gaaggaacag
    22441 cttttgagtt tgaaggtata gataccgcag ataattggtt aaataaagaa tcctttatta
    22501 aaagtatagc ggatgatctt gaaaaacaat ggtctaagga agactatgca gagtacctaa
    22561 aaacagaaag agatacagag gtgaaagaaa aactcactga ggtatgtttg gatgaacata
    22621 ttgatcccaa tatcgtaaaa caagaagact atgaagtggt tattgataaa gttcctactg
    22681 attttgagga agttgtaaag tatctagttg aagaagcatt atcgcaatac tcttcagatg
    22741 aggaagtgga cgaagaaact gttcgtacca gagtttggca actggttttt gatgacaaag
    22801 attataaata tttgagtgat caaaactcgg ttaattggag tgtacgatta ttaattgatc
    22861 aaaatgatat tgaataaaat agagctgaaa agctc
//
LOCUS       DASJUF010000043        22527 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJUF010000043 DASJUF010000000
VERSION     DASJUF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.043"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..188)
                     /locus_tag="U4B62_001459"
     CDS             complement(36..188)
                     /locus_tag="U4B62_001459"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2141624.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(379..1257)
                     /locus_tag="U4B62_001460"
     CDS             complement(379..1257)
                     /locus_tag="U4B62_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2141625.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1296..2414)
                     /locus_tag="U4B62_001461"
     CDS             complement(1296..2414)
                     /locus_tag="U4B62_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN2141626.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2411..4510)
                     /locus_tag="U4B62_001462"
     CDS             complement(2411..4510)
                     /locus_tag="U4B62_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN2141627.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4561..5382)
                     /locus_tag="U4B62_001463"
     CDS             complement(4561..5382)
                     /locus_tag="U4B62_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2141628.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5379..6188)
                     /locus_tag="U4B62_001464"
     CDS             complement(5379..6188)
                     /locus_tag="U4B62_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2141629.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6209..6703)
                     /locus_tag="U4B62_001465"
     CDS             complement(6209..6703)
                     /locus_tag="U4B62_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2141630.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6715..7203)
                     /locus_tag="U4B62_001466"
     CDS             complement(6715..7203)
                     /locus_tag="U4B62_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2141631.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7280..9976)
                     /locus_tag="U4B62_001467"
     CDS             complement(7280..9976)
                     /locus_tag="U4B62_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2141632.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10404..11477)
                     /locus_tag="U4B62_001468"
     CDS             complement(10404..11477)
                     /locus_tag="U4B62_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2141633.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11474..12301)
                     /locus_tag="U4B62_001469"
     CDS             complement(11474..12301)
                     /locus_tag="U4B62_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141634.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12302..13108)
                     /locus_tag="U4B62_001470"
     CDS             complement(12302..13108)
                     /locus_tag="U4B62_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141635.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13120..14205)
                     /locus_tag="U4B62_001471"
     CDS             complement(13120..14205)
                     /locus_tag="U4B62_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141636.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14222..14764)
                     /locus_tag="U4B62_001472"
     CDS             complement(14222..14764)
                     /locus_tag="U4B62_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2141637.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15022..16011
                     /locus_tag="U4B62_001473"
     CDS             15022..16011
                     /locus_tag="U4B62_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2141638.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16008..16745
                     /locus_tag="U4B62_001474"
     CDS             16008..16745
                     /locus_tag="U4B62_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN2141639.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16742..17293
                     /gene="coaC"
                     /locus_tag="U4B62_001475"
     CDS             16742..17293
                     /gene="coaC"
                     /locus_tag="U4B62_001475"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN2141640.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17309..17941
                     /locus_tag="U4B62_001476"
     CDS             17309..17941
                     /locus_tag="U4B62_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2141641.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18026..18673
                     /locus_tag="U4B62_001477"
     CDS             18026..18673
                     /locus_tag="U4B62_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2141642.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18678..19661
                     /locus_tag="U4B62_001478"
     CDS             18678..19661
                     /locus_tag="U4B62_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN2141643.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20064..21578)
                     /locus_tag="U4B62_001479"
     CDS             complement(20064..21578)
                     /locus_tag="U4B62_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN2141644.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21716..22522
                     /locus_tag="U4B62_001480"
     CDS             21716..22522
                     /locus_tag="U4B62_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141645.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         6804 a         4834 c         3904 g         6985 t
ORIGIN      
        1 aattacgatt tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag
       61 ccaaggatta tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact
      121 attaccacag acataaagta acacaatata ttactattta agtgtacgcc accagtgaat
      181 atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt
      241 cagtgattta atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc
      301 gctttatcca acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat
      361 caatgaaaca ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa
      421 tccgccgatt attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg
      481 tggtatttca acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat
      541 gtgttcttgt ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt
      601 tgtattgtat gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc
      661 caataaattc tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc
      721 ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt
      781 gatcaacata tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa
      841 ggcaccacct ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc
      901 agaaccagca acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg
      961 aatattgaaa tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg
     1021 tgttgatgtg atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca
     1081 gccgaccccg atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt
     1141 cattgtttca actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc
     1201 agtaccgact gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat
     1261 aggttcctcc cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca
     1321 gtgaaatgac gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca
     1381 acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc
     1441 gataaatctt cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt
     1501 ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct
     1561 cttgtggcat ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc
     1621 gcatgtggac gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct
     1681 tctgaacctg ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac
     1741 tcgccttcgc cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa
     1801 tagccttgac cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt
     1861 tcccgatgcc aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg
     1921 taatttatcg tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt
     1981 ttagcggatg tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc
     2041 actatcggca acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct
     2101 aaaggacttt caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca
     2161 cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt
     2221 attcccgttc cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg
     2281 ccacgacctt tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg
     2341 cctgttggat tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt
     2401 tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa
     2461 gaattttttt cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact
     2521 cggattgcgt acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa
     2581 acgttaaatt atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat
     2641 attgactgat cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa
     2701 agtctcgcac gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac
     2761 cgtctaaatc tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt
     2821 gcagctcgct cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc
     2881 aagtgatgcc tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca
     2941 tggtgttctg atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag
     3001 tgtctgattc ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac
     3061 ttaaaaagat taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg
     3121 ttttctcctc atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca
     3181 tttgtttaca aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac
     3241 tcagttgttc tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa
     3301 gattgctgcc ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa
     3361 aaagcattgg ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat
     3421 tcagtagaac ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa
     3481 gactagccag taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa
     3541 attttgaacc aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata
     3601 actccggttc aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc
     3661 gattagtagc aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac
     3721 cgaaactaga aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg
     3781 gcattgacag ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt
     3841 cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa
     3901 tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca
     3961 acacattttg ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt
     4021 gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct
     4081 tattagccag tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa
     4141 tatatctagg caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca
     4201 aatggtagac aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct
     4261 gtttattgac ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca
     4321 acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga
     4381 aggtcaatcc ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca
     4441 catttccgtc gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat
     4501 taatagtcat cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta
     4561 ctatgcgatt ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt
     4621 gatgacgccg acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac
     4681 aattccttta aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat
     4741 tgaaaagctc aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat
     4801 cagacccaag atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt
     4861 ttcaatatat ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta
     4921 attaggtata ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc
     4981 taagttgatc gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc
     5041 aattccagcc aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa
     5101 accagctttt tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc
     5161 gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc
     5221 ttcacttttg tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc
     5281 catcgtcacc ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac
     5341 caattttttc tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct
     5401 tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt
     5461 tttagttgta gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt
     5521 gcaatcaatg gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca
     5581 atatttttac tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt
     5641 ccagcgactg ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca
     5701 acatctgctc caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca
     5761 aaccacataa agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg
     5821 aaaaatttag cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat
     5881 cctaatactg ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg
     5941 attgcgaacg ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg
     6001 atattgacga cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc
     6061 aaatccccca tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac
     6121 atcccgaaca tttcatgtcc agcaactcca ataaaaacgg ccaaagcgac tacaaatgca
     6181 tcatacataa tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac
     6241 gtttttccat cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt
     6301 ttaagtcttg aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt
     6361 ccgatggcgg tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga
     6421 aagctttcgc aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt
     6481 ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt
     6541 tgacattcga tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct
     6601 catcgcttgc tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt
     6661 gtccatgaat taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa
     6721 aaaatcttcc tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg
     6781 ttggcattta tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac
     6841 tatttcgagc agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc
     6901 caaaaaggtt ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa
     6961 aatctgatcc tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca
     7021 ctcatccaca taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat
     7081 actgctaaga aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg
     7141 gttaattcct ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa
     7201 cattggttta atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt
     7261 agacactttt ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg
     7321 aaagctcata agcattgact ttaaaattat atttcatctc cattgattga aaaatacttc
     7381 tagaaacata ataaaattca tcttcatctg gtttcaaatc ataattcggt tcttcagatt
     7441 tttctgtccg agccaagaat aaacgttcca tcatcaatgc cgtatgcata tagagattta
     7501 gttttacacg gccatttaaa gtgaaatgat agtaattttc atatttagaa atgactgttt
     7561 ccacttcact catcacgatc gttgggttca gaaagttcaa acggtccgca attccttcca
     7621 acgaaaagaa ttttactagc tcctgaatca agcggttaaa ttctgccggc ttcatttcag
     7681 gactcaaaat atgctccaaa ttggctgctc catcaccgtc taagatgtca taaacattaa
     7741 tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt
     7801 caaaataagt ttcatcattt tggtcgatta attcacgtag ttctctgtag tccttcgtga
     7861 aaacttcgat ggattcatcc gataaatgat gacgaaaaac atctttcaat ttgtccgaaa
     7921 tccccaaacc cgacatacag gaaatgataa tgttgctact ttgagaaaag ccttcaaagt
     7981 attgaacatt gattgaatac ttgtctgctg catcctccgc aatctgttta aatggcgcat
     8041 tcatcgtcat cttcaagcca atatcaagtg caatggaagt cgtcaaatta ttcagaagaa
     8101 gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat
     8161 ctacgatcag cactagacta tctgtattca cttgccgttt cacgaaattc cttgtcgatt
     8221 cgacaatctc tgacacatta ctctctactg gcatatccaa cgcttcaaaa acataattgc
     8281 cgcataattg attcactact gcttgaatgc tgctagccgt actatctccg tgagcaagca
     8341 acaggccctt cagctcgatg gattcatcta cataatcaga taaagtcacg acaataagca
     8401 gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa atgctccaag aactgattac
     8461 ataagtaagt cgtccgaggt agatttgcag ctaatgtacg atttatcccg gttaagtctg
     8521 tcttatagct tggggaaatg acataggatc tgctaataat ttctgcaaca ggtccactcg
     8581 tcgtcaatcc gtacgttttt cctaaaacgg atacccagac ctttctaaac atctgaatca
     8641 tcaattttct actcattgat aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta
     8701 cttcattctt tatcgaagaa aaattttcat tgaaattacc ttcatttatc agctgagtaa
     8761 ttttttccaa aatcgaatca taatcaaacg gtttgattcg atccgttttt atcttttgat
     8821 ttttctttat caataaacta gcaatcggca agctttctac ttcggattcc agcatattta
     8881 actgaatcaa gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt cgactataca
     8941 catccgcaca actgatttta atgatattct tcagcttacc aatattccca ctaaagggat
     9001 gagataacat taaagacaag gcttctcttg aaacaataat atctttttgt aaaacatccg
     9061 cttcattttg aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa
     9121 aagttggcaa cataacacta atttgaatac gccgatcaaa ggtgtctaat aaataatttt
     9181 cacctttttc cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa tttacatttt
     9241 caccgactgg acgatagttt ccagtatcaa taaatagaaa gagcttttct tgattttctt
     9301 tcgataggcg atggacctca tcgagaaaaa gatagccgtt gttggccgtt gccaataaac
     9361 catcggtatc cttttcagcc cccgtaaagg cccccttctt gtatccaaac agtgttgccg
     9421 aaagcagttc gggattgtcc gcataatcgg cacagtttaa gatagcaaat ggtgcatctt
     9481 tttcaataat ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa aaactttttc
     9541 caacacccgt ggctccattt attaatacac tgagtccgtt aggcggatat ttgacagcgg
     9601 ctatacattt ttgaatgacc tctctcagac ttccgtcatg accaactaca gaatgaaagg
     9661 cttcgtttat ttgctccgta ttatctacaa ccgcatattc atctaaaggc ttccacaaaa
     9721 caggctttcc cggtaatttc tcgacctttt tatcttccac taatctcgtc aaataatggc
     9781 tggcattctg acgagagata cctagattct tcgaaatttt ctcggcagtg acagaagaca
     9841 tattccctgt tagtcgtatc agctcatcaa aaactcgatc tttagcgctc tttttcattt
     9901 tttcctccag tttaatgtgt ctaaaagcat aataaactat cggctgcaat atgtctattc
     9961 tatatttgta ttccaatttt tactttgaaa cacaaatagt cgaatatttg cctcagatat
    10021 ctgacaaaat aatttatcta ccaatttagt ttttatctaa tatgattttt atgttttttc
    10081 atagctacaa atattgaact ggattcatat attagtttta ttaatagatt acagaagggt
    10141 taagtatatt tgcatagttt ccaattactt tatccttctg taactttttc aatcttagat
    10201 ttactagccc gtctcgggcc ctattttaca atggttttgt ccacttttct ctggtagaaa
    10261 aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg tttcatccgc ttttggtaga
    10321 agattggaaa actcagatag aaaaaggacg atttttagct ctttagaacg ttgataagtc
    10381 cccattctta ctgagttttg ccattatctc ctatacatct tgaattctaa gaataggtca
    10441 ttatagatgc ctaggtattt cgatcctaga tctttgtaga cttcaagatg ggagatcgtg
    10501 tcatcagatg gatagaattg ttcgtccgac gaaatgtctt ttggcaataa cgcttttgct
    10561 tcttcatttg gtgtagaata accaatatac tctgcatttt gtgccgcatt ttccggttct
    10621 agcataaagt tgatgaaatc ataggcacct tctttatttt tcacggtttt tggcataacg
    10681 atattatcaa accaaagatt ggatccttct ggtgggatca cataatgtaa atgttcattt
    10741 tcccacatca tatcggcagc ttcgccagaa aatgtcactg ccacggaggc ttcttcattg
    10801 atcatataca ttttgatctc atctgccacg attgctttga catttggtgt taaatgattc
    10861 aatttatttg ctgcttctgt cagctgtgcc atattcttac tattcaaaga atagcctaaa
    10921 ctattcaaag aaagccccat aacttctcgt gcaccgtcaa tcagcatcaa actgtctttt
    10981 aattccggct tccacaaatc atcccaatgc ttaatttgat ttgcatcaaa gaatttatcg
    11041 ttatatacga tccctaaagt tccccaaaaa tatggaatcg aatactgatt atctggatca
    11101 aaatccaagt ctaaaaatcg tggatcaata ttatttaatc ctttgatctt cgaatgatct
    11161 aatggaagta ctagtttttc tttgatcatt ttttggatca tatattcact tggtatagcg
    11221 atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca tcgcttcatt agaatcaaat
    11281 gtttcataat tcactttgta tccatattct ttttcaaatt tcgtgatcaa actaggatcg
    11341 atataatctc cccagttata gatggtaagt acctttgctc cactcatgcc acttgttttt
    11401 tcaagttgac gaacaccaag cagcagcacc gcaataatga tgagaatacc taaaaacaga
    11461 gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg tactcttctg tttttcttca
    11521 gccgttttgg acggttttct ttactaataa agtagtatcc aatgaccaat atcatggaga
    11581 acaagaaaac taacgcactt agcgcattga tttctaaact gattccttgt cttgcacgag
    11641 aatagatctc tactgagagc gtcgtaaagc catttcctgt gacgaaaaat gtaaccgcaa
    11701 aatcatcaag agaataggta aatgccataa aatatccagc tatgattcct ggagaaagat
    11761 aaggcaaaat gatatttttg actacttgga aattattagc accaaggtct ctagccgcat
    11821 cgaccattga atcgttcatt tcctgcaatt ttggcaagac catcaagacg acgattggaa
    11881 tgctgaaagc aatatgggaa agcaatacac tctgaaaacc taatccgaat cctagtagtg
    11941 taaagaaaat caagaaacta gcaccgataa taacatcagg agagactaat aagatgttat
    12001 tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt gtaatagatt cccatagccc
    12061 caaatgtccc gatgatcgtg gcgatcaatg cagataagaa agccagcatg aacgtattca
    12121 atacgataat gatcaaacgt gtatcttcaa aaacagcttc ataattttcc caagtaaatc
    12181 ctgtaaagct gttcatcgtt cctccttcat tgaaggaata aaaaatcaga tagaagatcg
    12241 gtgcatataa gaataaaaag acgatcccta aataaagata cgaccattta aattttttca
    12301 tttatgagcg acctcctttt ttcttctcgc ctgtcaggag catcacgatg aacatagcaa
    12361 tgattaaaat cacaccgatt gtagaaccca tcccccagtt ttgtgtaacc aagaaatgct
    12421 gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc aatcagacga gtcagcatga
    12481 ataaagaaag agaaggaata aatactgctt ggacaccact tttcacacca tttaatgata
    12541 acggaaaaat cactcgtcgg aaggtctcaa agctattcgc gcctaaatct ctactagcac
    12601 tgatcaaaga aggattcatc tcttccaatg cattgaaaat tggcataatc ataaatggaa
    12661 tctcgatata cgttgccacg aacagaaaac tgaagtctgt gaataatatc tgtttcggcc
    12721 cgatcccgat aaaatcaaag aaatgattca cgctgccgtg cacactgaaa atcccaataa
    12781 atgcgtaagc tttaagcagc agattcaccc atgtaggcaa aataatcagc atcagccata
    12841 attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg gtaactgatg atcagcgtga
    12901 aaaacgtgat caaaaatgca taccatacag aattgatcgt catagaaagc gatgtccccg
    12961 acgtaaaata agatagataa ttattcagcg tgaattgccc attcatatca aaaaaagatt
    13021 gataaatgat cattaataca ggcgcgatca cgaataaacc gagccacaaa atataaggga
    13081 cggaatagat cctgcgcatt gttttcattc ttgtcccccc tattcttcgt agctgtcaag
    13141 acgtgcatcg aattcttctt cagactcgtt gaaacgcatg acatggatat cttctggctc
    13201 aaaggccaat cctactttgc ttccttcttt agcttttttc gttgaatgga ccatccattc
    13261 attttgctgt tcatcataac agatgatttc ataatggaca ccacgaaaca gctgagtatc
    13321 gaccgttacg actagttttc ctttatctgg tgtcgtgatc gtcaaatcct ctgggcgtaa
    13381 aacaacttca actggttcat ttagacgcat cccgccatcg acacattcaa attgcttacc
    13441 aacaaactca actagattat cttcgatcat tacaccgtcc acgatattgc tctccccaac
    13501 aaaatcagca acgaaatgat tgattggctc atcataaata tctacgggtg taccactttg
    13561 gacgattttc cctttattca tgacaaaaat ctcatcactc attgccaacg cttcttcttg
    13621 gtcatgagta acaaaaataa acgtgattcc taatcgttgt tgcagatccc gcagttcata
    13681 ctgcatatct gtacgtagtt tcaaatcaag cgccgataac ggttcatcta atagcaatac
    13741 tttcggttca tttacaattg ctcgtgcaat cgctacacgc tgtctttgac caccagacat
    13801 ttcactaatc tcccgttttt cgtatccagg aagctggacc atacgcaaag catcttttac
    13861 cttcttttcg atatctgatt tggacatttt cttgatcttc aatccaaaag caacgttatc
    13921 aaaaacattc atatgaggaa aaagtgcata atcttggaag actgtattta cttgtcgctt
    13981 attagctggc aaatcattca accgtttccc ttcaaaatag atgtcccctt ctgtagcttc
    14041 ggtgaatcca gctataatac gtaggatcgt tgttttacca cagccggaag gaccaagcag
    14101 tgtataaaat tttccttgtt cgatctcaaa acttactttt tttaatactg tttcatcatc
    14161 atagcgtttc actacttgat caaatgaaat gattggtttc cccacgtttt taccccctat
    14221 attacaaata tgattgcgtt gcaacaatca atacttttgt tcgttttttg agatgattcc
    14281 ttaattgatg ttgttttgtc gcttgataat agatcgactg tcctgctttt gccacataga
    14341 ccgtctctcc taagcttaac tctaattccc cttctaatac aaaaataaac gtttcagata
    14401 atgaaggttc aaacgtctta tattctcctg caccttcaaa agtcagcaag acaggctcca
    14461 tttctttttc attcgaatca gccacgagcc attccaattc atacccgttt tcttcatcga
    14521 aataaaccgt atgatcttct tttgaataaa ccacttgcag attcgctgct tcttgatgaa
    14581 aaaattcttc tggagtcaca cctaagactt caagaataga aaagaatgtt tccattgatg
    14641 gtgaacttaa atctcgttcc aactgggaaa tatacccttt tgacaaatct gtgcgttcgc
    14701 ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt tcttaacttc tcaccaattt
    14761 ccataatttc ctccgaatcg aagttttcta atagtaaact tcctgtttac taccactcgt
    14821 attagtatac gaaatttatt gaaaaaggca accctttgta aaattttttt tgctgaatat
    14881 ttctttatct tttggaataa attctagttg tttgattctt tatttttcac ctaatttttg
    14941 tctaaattta cggaagaaca agaataaaag agtaggcttt atggtatgat aaaagaaaat
    15001 tttaaaagga gtgtctcacg aatgaacgct tttcgatctt ttcaggtaat aaaggaacct
    15061 gttttccata cagcaatcag tgatctgcca tttactgagt tggctgaaaa tgaagtcttg
    15121 gtaaaaattg cctattctga tgtcaattat aaagatgcat tggcaatgag cgagtctggt
    15181 caggtgatac gaacatatcc gatgactcca gggattgatt tatccggaac tgtcgtccaa
    15241 agtaacaatc cacgtttttc aaaagaagat cttgtacttg ctactggttt tgggcttggc
    15301 gtcacccatc caggcggtta tagccaatat caaaaagtac caggagactg gttagttcct
    15361 ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg gtacagcagg gtttactgct
    15421 atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct ccgataaaaa agttgtcgtt
    15481 actggtgctt ctggcggtgt aggcagtaca gcgatagccc tgttacataa actaggattc
    15541 cagtcaatca ttaccttttc cagaaaagaa gagtctgtca catggctaaa aagtctaggt
    15601 gctttgcaag tcgtacgacc tgatgaattt ctgccagaga caacaaaagc tttaggaaaa
    15661 cagcaaatcg actatgtcat cgacacagta ggtggcgagc agctaagcag tcttcttcca
    15721 ttgatctctt ataacggtgc agtagcttta tgtggcaatg ctgggggaat caaactaaac
    15781 gcgactgtct tgccttttat tctgcgaaac atccagttga ttggaattga ttctgtcaat
    15841 gtaccaatag atcaacgtct ttctttatgg caacagatgg cggatttgca gattgcagat
    15901 gaactggtcg tccaagagat tactctggat caattgcctg aaacagcatc taaactactt
    15961 gctggaacac accaaggaag aacactagta aatgtaggtg accataaatg aagattctaa
    16021 tcacagctgg aggaacgact gaaaaaatcg atcaagtacg tgctattaca aatcattcga
    16081 caggacgatt agggcaagca ttagctgatt atttagctgc caatccggat accacaatcg
    16141 attacgtgac tacgaaacaa gcactgaaac cgaaaagaca ctccaatata acgatttata
    16201 cgatcgaatc tgcactggac ttatttttac aattagaaga actaacgaaa aaagaacact
    16261 acgatgcgat tatccacagt atggcagtca gtgattttac tcctgctttt agtttttcag
    16321 aagaacagct ggcaaaaaac ctacccactt cttcaacgca agaagaactg gacaattggt
    16381 ttgcggaaaa tgaacagaca gataccactg tttcaaagat ctcttctaac accgagcatc
    16441 ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta tctacgtgaa tggcagccaa
    16501 aagcaaaaat cattggtttc aaactattag tagatgtacc aaaggaatct cttctggcag
    16561 tcgcaaaaaa tagtctgata aataacaaaa ctgattttat tttcgcaaat gatttgacgg
    16621 aaatccatgg cgagacacac cacggctatc tcttatctaa agatggaaca gtggaagaag
    16681 ctcaaagtaa atctgagatt gctgcattga ttactgagaa aattagactg gaggaatcaa
    16741 aatgaaaaag atcttactag gcgttactgg aagtatttct gcttacaaaa gcgctgatct
    16801 tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg attatgtcta aaagcagcac
    16861 acattttatc acgccattaa ccttgcaatc tctgtcgaag cgcgcagtcc atacagacgt
    16921 catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa ctagcaaaac aagctgactt
    16981 gtttctcatc gcgcctgcta cagcaaacat tatcggcaag ctagcaaacg gcattgcaga
    17041 cgatcttctt tcaacagtcg caatggcgct tccagtggga acaccaaaat tgattgcccc
    17101 tgccatgaac acgaatatgt atcaaaaccc gatcactcag cgtaatctgg aaacattgag
    17161 atctgtcggc tatcaggaaa ttgaaccaag agaatctctt ttagcttgtg gagattttgg
    17221 aaaaggtgcc ttagctgata ttcagacgat catcgaacag gtcatgctga ctcttaatga
    17281 aagaaatgct tagaaaaaag gagtacttat gaaaaataca aaaaacttta cattaactgc
    17341 gatgttttta gctattttga ttttattagc tgttacgcct ttaggtttta tccctatcgg
    17401 tccaatcaat gcaacaacca tgcatattcc tgtaatcatt gcttcgatcg ttttaggacc
    17461 aagactaggt gcttttcttg gcggtacatt tggactgatc agcatgatcc gtagcacagt
    17521 tatccaaaca cctttgtctt ttgtcttttc tccgtttatc ccagtcatcg gaacagaaca
    17581 tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc atcttgattg gggtggttcc
    17641 ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa aaagcagatt ccgtttcttt
    17701 attcttagct ggactttccg gttcattgac caatacgatt ttagtgatga atatgatcta
    17761 ctttttattc cagcaagatt atgccaaagt catcggaaca aatttaaatg ctgtttattc
    17821 agctattcta gcggttattt ttacaagtgg tatacctgaa ggaatcgtcg caggtcttgc
    17881 gactgcagca gtatgcaatg tcttgttaag atacttcaag cacccttctc ttactaactg
    17941 atagtcatcg tagccgatct ctgatttttt tctcttttgg tgttactatt gaatggaaag
    18001 atttgacgtc gaaaggatga tccaaatgaa aatttttgtt gtaggtgcaa acggacaaat
    18061 tggccgacac ctgataaaag atttagctcc aagttcccat gaaatttttg caggggtgag
    18121 agatgttgct tcccaaacat tagtaaaaaa agagaatgtt tcgtatgtct cttttgattt
    18181 aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc atcgatgtat tgatttttgc
    18241 tgccggctct caaggaaaga atcttctgca agtagacttg gatggcgcaa tcaaaacagt
    18301 gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg gtcagtgctg tttatgcaga
    18361 tgaaccagca aaatggcctg agtccatgac agattattat attactaaac attatgcaga
    18421 tgaatggctg aaacgaacta acttagattt tgtcattctt caaccagtca cattgactaa
    18481 cgatgaagaa gttacttcca ttcaattaac taagcctaat gagaaggcat caaaaacgat
    18541 cactcgctct acagttgctg ctgtacttgc cgctctagtg gaagaaacgg atatttctcg
    18601 tacgactcta gtactttctg aaggtagcaa agagttgaat acagcattcc aagaatgggc
    18661 aaaggaggaa tagatccatg cgtgccattt caatcaagca tcctggttct gtagatgtat
    18721 tagaacaaat tgaagtgcct gttcctaaac caaaacaagg cgaattactg atcaaagtcc
    18781 atacagcagc tgtcaatcga acagatatta tgaaaagaga atcagtctct ttgaaacctc
    18841 cctaccctat tttgggagtc gaagtagctg gagaggtagt tgagaataaa agtgatatct
    18901 ccacttttac tcctgggaca aaagtatacg gattagtcaa tctaggtggt tatgccgaat
    18961 atgctgtgat gcctgccgat cgtgcaatcc ttctccctga tacgctagac tatgtttctg
    19021 ctgctggaat ctcagaagtt tttttgactg cttatcaaac actttactgg cttggaaaat
    19081 tacaagaagg cgaaactgtc ttgatccatg caggagcaag tggtgtggga acagcagcga
    19141 tccaattagc tagacagctt tctcacgcga aaatcatcgt tactgcagga tcagaagaaa
    19201 aactcgcctt ttgtaaagaa cttggtgcag atgaattgat caactataaa aaacaagact
    19261 ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt cgatgttatt ttggatttca
    19321 tcggcgcttc ttactgggaa aagaatttag cttctatagc cgttgatgga cgttgggtct
    19381 tgattggtat gctaggtggg accatcgtgc ctgagataga ccttggtgta ttgattggga
    19441 aacgtgtcca actgcttggt acattattga cacctagaag tgatcagtat aaagcagagc
    19501 tcgcacaaac ttttatgcaa aaagctgggc cttttctaga aaatggcaag attcggccaa
    19561 tcattgaccg tacattccaa ttcagcgcag tcaaacaagc gcatgaatat atggaagcaa
    19621 acaaaaacat cggaaaaatc attttgaaga taactgaata aaatcatgct gatcaaaaaa
    19681 gaggctggga cagaagcgtt taactccaag aaataagaag aaattcacga aaattgcttt
    19741 tcaaattttt gtgaatttca gcttatttcc gaaggaattt cttctgcttc cgccgtttat
    19801 acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc tttttccgaa
    19861 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag
    19921 aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
    19981 tcactaaaca gtgttcaatc agctgaccct cggtattagc tcaaaacgct gatttcacaa
    20041 cctcttttta tttacgttct atttcattca ttttccgaag caaatcgagc tgcttgtatg
    20101 cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat ttccaccgat ccggttgttt
    20161 ccatgtaagc cacctgtgac ttcaccagca gcatataacc ccctaattgg ctggttttct
    20221 ttatctaata cttcagtgtt cgtattgatt ttcacaccgc ccatcgtgta atggattcct
    20281 ggtgcgattt tgatcgcata ataattcgga gacgataagt catgttccat ggctgtggca
    20341 cggttgaatt gagcatcttt tttctccgca acatccgtat tccatccatc aactgttttt
    20401 gctagtgttt cttttggtaa atcgattttt tctgctaatt cttccattgt ctttccagtt
    20461 gtgacatatc ctttttggtc ataaaattca atagctgtgg cacgatcacg cacgccttgg
    20521 tcaaaaatca aataagctga tttttcaggc aatgctgtaa ttgctgcaga tactttatcc
    20581 cttgtatcca tttcattgac aaatcttttc ccctcttgat cgaccaagat tgctccttct
    20641 ccgcgaacaa cctcaccaat caagaccccc tcatcctgct gcacagttgg atgaatttgt
    20701 attttatcca tatcaacggt ttgtccacct aatttttcaa tcatggtgat accatctcca
    20761 gttgatcctt cttgatttgt cgtaatataa tcagctaaat cgggacgata tttttcaata
    20821 aattctttgc tggcaccaaa accgcctgtg gtaactacga ttgcttttcc ggtgacctct
    20881 tttggttctt ctccttgaac atgtacagtc acttcattga ccgtttcccc atttttctta
    20941 atgtctgtca catctgcgtt gacaaaaata gggatttccc gctcatggac gttattcaat
    21001 aacccctcca ctaagtatcc accaatagcc gagccatcag acggacgatg tgtcctcttt
    21061 tcactcatcc ctccagtgat cgttaagttg tccagtttga taccgttttg atctagccaa
    21121 tcgaccgcat cagcagagtg atcgacaaag tatcgaagca tttctttatc attcgttcct
    21181 ccaccacctt ttagcgtttc ttcatagaag gcatcattcg agtctttgat tccttcttct
    21241 ttttgaaatt ttgtttctga agcattcata ccgcttgatg atttagatgt gtttcctccg
    21301 gcaacaggca tcttttccaa aattactgga ttcaacccag catcttttgc ttcgatagct
    21361 gctgccatcc ctgcaccacc agctccaata atcacgacat cataagattc tttcatttct
    21421 tttggatctg tatatacttg ttcagaagcg cctgatgata cttcggcaga ttccttcgta
    21481 ctagaacttg tctgcgctgt atttttctga tctgcgccgc atgcgctcat agcaagaagt
    21541 gcaacggctg ttaataaacc agcaactaat tttttcattt gtatccccct tttctcctta
    21601 tactatatta agtgattttt ttcacaatat caactctgtt tttctttaag tcatatagct
    21661 ttggtaaaca cacgcttctt agtataatta aggatataca tgaaggagtt attttatgaa
    21721 tcatttgaaa acctttttta cgaacagtac aaaaactgca ggtagaatca caaacggaat
    21781 ctatatttta ttacgtatca tcgtcgttat tatgctagtg gatcaggtct ggaaccaaca
    21841 ctggggaaat acaatgcttt tattactgac attaatttta tttgaaatcc cttattttct
    21901 ggaaaagata ttaaagatac agattccaaa tatgctagaa atcattttgt tatcttttat
    21961 ttttagttca acagttttag gagagttaag tgatttttat ggctattttt caatgtggga
    22021 tacttttcta catgcattca gcggattttt agctgggggt gtcggctttt cccttgttta
    22081 tttgctcaat aaaaatacaa aaaccatgaa cttgactcct ttattactag cagttgtttc
    22141 cttctgtttt tcgatgacag ttggtgttat gtgggagttt gtggaatttt cagcagataa
    22201 attactgcaa ttcgatatgc aaaaagatac gatcatccag caaatcaata gtgtggagtt
    22261 aagtaaagac ggaaacactg tctatcatgt tccagatatc actcggacaa tcattgaaag
    22321 tcaaaatgca gatggtaaaa cagataaaac aatcatccaa aatggttact tagacgtggg
    22381 aatcatggat actatgaagg atttatttgt taattttatt ggagcagttg ttttcagtgt
    22441 atttggttac ctctatagtc gaaacgatta ccaaaaattt acgttcatca agaattttat
    22501 tcctaaaaaa atcaaacgat aaaacat
//
LOCUS       DASJUF010000044        22301 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.044, whole genome shotgun sequence.
ACCESSION   DASJUF010000044 DASJUF010000000
VERSION     DASJUF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.044"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(27..878)
                     /locus_tag="U4B62_001481"
     CDS             complement(27..878)
                     /locus_tag="U4B62_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2141646.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1001..1468
                     /locus_tag="U4B62_001482"
     CDS             1001..1468
                     /locus_tag="U4B62_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN2141647.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1731..2213)
                     /locus_tag="U4B62_001483"
     CDS             complement(1731..2213)
                     /locus_tag="U4B62_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN2141648.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2313..3176)
                     /locus_tag="U4B62_001484"
     CDS             complement(2313..3176)
                     /locus_tag="U4B62_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2141649.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            3774..4649
                     /locus_tag="U4B62_001485"
     CDS             3774..4649
                     /locus_tag="U4B62_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141650.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(4794..4946)
                     /locus_tag="U4B62_001486"
                     /pseudo
     CDS             complement(4794..4946)
                     /locus_tag="U4B62_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(5214..6545)
                     /locus_tag="U4B62_001487"
     CDS             complement(5214..6545)
                     /locus_tag="U4B62_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2141651.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(6694..7407)
                     /locus_tag="U4B62_001488"
     CDS             complement(6694..7407)
                     /locus_tag="U4B62_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2141652.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            7582..9006
                     /locus_tag="U4B62_001489"
     CDS             7582..9006
                     /locus_tag="U4B62_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2141653.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            9305..10012
                     /locus_tag="U4B62_001490"
     CDS             9305..10012
                     /locus_tag="U4B62_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2141654.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            9999..10322
                     /locus_tag="U4B62_001491"
     CDS             9999..10322
                     /locus_tag="U4B62_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2141655.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(10411..11448)
                     /locus_tag="U4B62_001492"
     CDS             complement(10411..11448)
                     /locus_tag="U4B62_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2141656.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(11769..12752)
                     /locus_tag="U4B62_001493"
     CDS             complement(11769..12752)
                     /locus_tag="U4B62_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN2141657.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(13167..13937)
                     /locus_tag="U4B62_001494"
     CDS             complement(13167..13937)
                     /locus_tag="U4B62_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141658.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(13930..14865)
                     /locus_tag="U4B62_001495"
     CDS             complement(13930..14865)
                     /locus_tag="U4B62_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141659.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(15181..16830)
                     /locus_tag="U4B62_001496"
     CDS             complement(15181..16830)
                     /locus_tag="U4B62_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2141660.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(16947..19550)
                     /locus_tag="U4B62_001497"
     CDS             complement(16947..19550)
                     /locus_tag="U4B62_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN2141661.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(19562..20611)
                     /locus_tag="U4B62_001498"
     CDS             complement(19562..20611)
                     /locus_tag="U4B62_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN2141662.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(20637..20951)
                     /locus_tag="U4B62_001499"
     CDS             complement(20637..20951)
                     /locus_tag="U4B62_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN2141663.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(20967..21413)
                     /locus_tag="U4B62_001500"
     CDS             complement(20967..21413)
                     /locus_tag="U4B62_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2141664.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(21414..>22301)
                     /locus_tag="U4B62_001501"
     CDS             complement(21414..>22301)
                     /locus_tag="U4B62_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2141665.1"
                     /translation="GVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIA
                     YVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQ
                     DRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKR
                     FCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEV
                     DFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLA
                     NLKTSSSFEKLVYILKEGL"
BASE COUNT         6773 a         4543 c         3633 g         7352 t
ORIGIN      
        1 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
       61 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
      121 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
      181 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
      241 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
      301 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
      361 tcacgtttca atacccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
      421 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
      481 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
      541 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
      601 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
      661 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
      721 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
      781 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
      841 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
      901 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
      961 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     1021 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     1081 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     1141 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     1201 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     1261 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     1321 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     1381 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     1441 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     1501 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     1561 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     1621 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     1681 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     1741 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     1801 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     1861 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     1921 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     1981 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     2041 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     2101 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     2161 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     2221 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     2281 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     2341 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     2401 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     2461 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     2521 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     2581 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     2641 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     2701 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     2761 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     2821 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     2881 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     2941 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     3001 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     3061 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     3121 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     3181 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     3241 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     3301 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     3361 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     3421 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     3481 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     3541 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
     3601 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
     3661 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
     3721 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
     3781 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
     3841 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
     3901 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
     3961 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
     4021 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
     4081 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg caatttatcg
     4141 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
     4201 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
     4261 tcatggagat gcgaaagttc atccaagaag cagttgaatc gctcattcag caaaatgcct
     4321 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
     4381 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
     4441 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
     4501 tcgatcatac tgacgcctct tattcaaaac gctactccat aaaaatcctg aaagaagact
     4561 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaaact aaagaaacgg
     4621 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
     4681 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
     4741 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
     4801 tcatgacaaa tttccttcgt tgctgggtag attttccggc attgttgata gattttatga
     4861 tacttttcta cattttgtaa gagaggttcc actgcatctg tatattggat aaaattctga
     4921 caacaacttt caggtgtgtc gaaccactct aatctttaaa gataaatatt aaaataacga
     4981 ttaataatat taacagtctt tatttttcac caccaaaaac ctatctttgc atatactaaa
     5041 tagacgtata tacacagata ggtttgtctt gtgaaaaata atgattatcc ttaccaaaaa
     5101 atgtttgttt ttctacatgt tcgtacttat caaaaaatat gatccttatt gaaattactg
     5161 gctatttata ttccaacgac ttcttgtgtt aatttttgtt atctcatagt aaattatgtt
     5221 tcattttcac ttaatttagt tgtcacgtgt tcgtcaattc gtaaaaacgg taaatatata
     5281 aacacacttg cggctataat taatatttgt aaaacagctg ctcgccagtc tccggctgtt
     5341 gctaaaaatc ccgatataac tggtggcgta gtccaaggaa tttgcactac tacatgatta
     5401 attaatccca cagcagttgc aaaatatgct actgtcaaag aaaaaatcgg tgcaagcaca
     5461 aagggaataa tcagactgat attaaataca ataggcaatc caaaaataat aggttcgctt
     5521 atattgaata tagcgggagt taccgataat ttagcaaagt ccttataatc ttttctacga
     5581 ctgaagataa atattgcaat caataaagca atcgtatttc ccgatccacc cattaccgca
     5641 aaggtatctt taaaggccat attgataata tgtggaattt gctctttgtt tgcataagct
     5701 gccatatttt cttgcatatt ctgaatcaaa aacggatcta acaatgaacc tgatattact
     5761 gcttgatgga taccaaatcc aaaaaataga ttagcaatag atgtaattaa caaaaatcct
     5821 ggtaaaccag ttgtaatttt acttaatggc tctgtaacta tcgtggtaat tagcttattt
     5881 atatccatat taaacaattg aatgattata aatgacaata aagcaaacga ggtaatcgta
     5941 ataatgattg ggattaatac attaaaagac tttaaaacag cgggaggaat atttccggat
     6001 agctgaatct gtagtttctt attattagaa agtttaataa ataattctgt agccgctaat
     6061 ccaacaaata tacctacaaa cattcctgaa gctcctgtat aggatatagg tatcacaccc
     6121 gtcacttcaa cagctttatc cgcattttca ggagtaaaca tcatctgcat aggtgttaat
     6181 acaacaaatg tcgaaatagc aactaatacc gctgcaatat gatttgtata atttctgctt
     6241 gcacacatat gataagatac tgctgctaca actaataatg tagttatgct taatgtacca
     6301 ttggcaattg aagtaccaat ttgttgtaac gtggtcaatg tactagtatc gacaatttcc
     6361 cccatgaatc ctgttggttc taagatcact gaattaataa gtgtcacaaa tcctgctaac
     6421 atcataaatg gcataaatgt agcaaacgca tctcgcaaag ttcttaaatg gatctgattc
     6481 cccaacttag tcgcaactat accaaatttt tcaaatacaa tgtccttcct gctacttttg
     6541 cccatatttt ttctccttta tgcttattgt aatcgattac tttttccacc caaataatac
     6601 atcacttatc aaaaaaagta aatactttta attaaatata tatatctttt ttagtataaa
     6661 aaaataagct ctgaaaaaat aattttcccg agcttatttt tcaacaaaaa cagcttcaaa
     6721 aacaaagtct tgatataaat gtctcgatac cgaatactcg aaaggtttac cagacgatag
     6781 agcaacaact ttgtctatct caatcataaa ttcacctgga tctattttta atatttttgc
     6841 gtcaaattca ttcgcagtat ctcctttgat acgtatgtgc gaagtcttta tttccagacc
     6901 cagttcattt gtgatataag catatacaga tttctttaaa tgttttggtt ccaagcctgg
     6961 tatgactgct aatggcatga atgtttgctc aatcgagtat ggttgacaat caataaaacg
     7021 gcttcgtaca atattatata catagtcttt tacactgata cttaatttat cagctacttt
     7081 tttcgatgga agttcaattg agaaagtatg gatttctgaa tctacgtgtt cttcaccaac
     7141 cacgctaaac aaaccttcgt ttggaccaag aactcgtgag ttatcaattg gtgttctgac
     7201 gacttcagtc ccatacccac ttctacttcg caaaactcct tcggaaacaa gtaagtctac
     7261 gcctttcttt actgttaact tgcttgtatg ataaataact gccaattcat ctcctgtagg
     7321 aagtttctca ccaattttat actctccatt tacaattttt ttctttatat ctaagtaaat
     7381 ttccatatac ttcgcaattt ttttcaatgg tgaccctcca atcaaaaaca tatcttcatc
     7441 tatactttaa cataaataaa ccgataaaat ccacatcaaa attatacaca caataaaaaa
     7501 gatatatata tttaaacaaa actatttact ttaagttgta agcggtgtta tcattaagtg
     7561 gaaacaggag gtaaaaaaat tatgacaact atgaaaaagc taacttttcc agatgatttt
     7621 tggtggggtt ccgcttggtc agctgagcaa gcagaaggaa aaggagaaac aggaaaagca
     7681 gagactatct gggatcgctg gtttaaagaa caaccttacc ggttttatca acgaatcgat
     7741 tcctctatta caaccgatca catccatcaa tataaagaag atatagaact aatgaaaaaa
     7801 actgggcata actcctttcg cgtttctatt tcttgggcac gaatgtttcc aaatggcggt
     7861 accggaaaag taaatcccaa agccatttct ttctatcgaa aattattttc cgaaatgaat
     7921 aataacggaa tcaaagtatt tgccaactta tatcattttg acatgcctgc tactcttcaa
     7981 gataaaggag gatgggaatc acgtgaagta gttgatgctt atgttcatta cgctgaaact
     8041 tgttttaaag aatttggtga cttagtttgt cattggttta ccttcaatga accacttggg
     8101 cctattttag gtacttatct tgaagatttt cattatccaa acctcattga ctttaaacgc
     8161 ggagcgcaag cagcattctt cactatactt gcacacgcaa aagcaataca agtatttaaa
     8221 gaactagctc ttcctagtaa aataggcgtt attctaaatt tgagtcctac ttttcctcgt
     8281 agtcaaaatc ctgctgatgt gaaagcagca gaaatcgctg atttatttta tacgcggagc
     8341 tttttagatc ctatggttaa agggaatttt tctaaaaagc ttatcctgtt attacaagaa
     8401 tataatcagc ttcctgaaga ttatacgaaa gaagatcttg tatgtatcta cgaaaatacc
     8461 gctcaaattc ttggactaaa ctattacgag ccacgtcgcg tcaaatctcg ttcttctatg
     8521 gtggatacat ctagtccatt tttaccagag tggtttttcg aaccacatat catgcctgga
     8581 cgacgaatga acgaataccg gggatgggaa atttacgaaa aaggaattta tgatttgtgc
     8641 atggatatca agaaccatta tggaaacatc gaagcattta tctcggagaa cggaatgggc
     8701 gttgcggatg aagaacgttt catggatgat acagggcaaa taattgatga ttatcggatt
     8761 gagtatatca aagatcatct tgcttactta tggaaggcaa taaatgaagg atgtaacatt
     8821 aaggggtatc atctttggac atttattgat tgctggtcat ggatcaacgc ctataaaaac
     8881 cgatatgggc tggtttcact cgaccttact actcaaaaaa gaacaattaa aaaaagtggc
     8941 gaattttaca agcaattaag tgatacaaat ggatttagat acgatgtaga cttgttagtt
     9001 gaataatatt ctaaaattcc acttgaagtg aacacattag aaaacaagaa taatgagaaa
     9061 taccaataac aaatactttt tcttcatact tcatttatac ttgtcagtat aagagactgt
     9121 gacaaatgta gtgatcctca gaagttaggt ctggaaatct aacttgagaa gattttttta
     9181 aactacgtcc agtctttttt atttgtttaa aattgaagtt gacaagtctt cattaaaaat
     9241 ttagaatatt cacaagtttg acgacaatta tgaaaattta tgtcgtgcta aaggagtttt
     9301 gttattggat gaaaagttag atataaaaac tgcgatcaaa gacacgttac ctaccgtttt
     9361 tggttatatc ggtattggac ttgcatttgg tatcgttggg aaagctgccg ggtttcatcc
     9421 gctggtcgtc acgttgatgt ccctgctgat ctatgctggt tctgcccaat ttatcacggt
     9481 cagcatgctt gctagccata gtccactgct ctccatcgtt ctctcgacct ttctagtcaa
     9541 ttcccgaatg attctgatga gtatgacgat tgctccttat ttcaagaaaa atcggctgct
     9601 tcaaaatctg ctgattggca cattgctgac agatgaaagt ttcgcactgg gaatgaataa
     9661 attgaactat actggacaaa agttaaactt tcgttggatg aatacagcta attggatttc
     9721 ttatttgacg tgggtgggtt cttctcttgt tggcgcactt ttagggaatt ttattactga
     9781 tccgaaaaag tttggtttgg atttcgccat cgtcgctatg tttatcgggt tactttatct
     9841 tcaagtcatt tctgatagaa acacctctaa acgattacag ttgatcctga ttggtctcac
     9901 tttgattctg gtatatgtcg gactgatttt tatcccaagt aacttagtga ttttagttgt
     9961 aacactgatt gggtgcggat tgggggtgtg gatcaaacat gccttctttt gagtttacat
    10021 tgctgactat tttaggttgt actttagcaa cttggctttc taggatccta ccatttgtct
    10081 tactgaaaaa attcgatttg ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg
    10141 tcattatgtc tgctttatgg ttcagcagtc tatttaccca aaatatcgga catctgccgc
    10201 agatcaatct cgaaaatgct ttggcttcag ttcctacact tttagctgct attctttcaa
    10261 aaagcttatt ggtgatcgtc ttggtaggaa ttctttccct ttcgttgatt cgcttattat
    10321 aaataaaaaa acaagagctt atggcaaatt actgtcatgc gctcttgttt ttatcaaagt
    10381 caaataagaa aaaaatcggc gtattcttgc ttatccgttg aacggaccac taacaacgac
    10441 ttttcctaat atatgtcctg tttctacgtc tttggttgct tgtttcaagt tcttcgcatt
    10501 catcccatct gaatagacct tgctcaatgt actgcggata atcccttcat ttgctaaagc
    10561 agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca tagttatagt ctgttttagc
    10621 gaacatatac tcccagtcga agctgataga ttggtttttc caaacaccta tatctaacgg
    10681 ctctttgatc cctacgatcg ttcccacatg tccaaatggt cggacaaggt gtttgatttg
    10741 atcaaagtag cgggtaatat cgaataatac agcgacatac tctacttgat cgattcctaa
    10801 ttcacgaaaa cttttactta agtcgtgatg ataatcgatt ggatagtcta cacctgtttt
    10861 tttcagccat tcaaaattct tcggacttgc agtcgcgtag acttctaggc cagcccaatg
    10921 cgctagttga ttcaaaatcg aaccgacgcc cccagctccg ttgatcacta agattttttt
    10981 cccgcggttg gcattttctt ctggagtcag accaaatttt tcaaataaca attcataagc
    11041 ggtcaatgag gtcaacggca aggctgcggc ttcttcatct gaaagatttt ttggtgcaag
    11101 agcgacaata cgttcgtcaa ccagttggta ttcttgattg cttcctgctc ttgtcgttgt
    11161 acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc acttcatcac ctacagcaac
    11221 aactttcccg actccatcaa accctaagat acgtaaagca tttcttatcc cgttatcttg
    11281 gcgaagtttc gtatcgacag gattaacaga tactgcgttg acttttacca gaagatcccg
    11341 tgaactgggt acaggtatct gttctttttc atcaataaag ctttttggat catcgatcga
    11401 caacccttca taaaaaccga ctaccttcat taattccttt tctgacatac gaattcctcc
    11461 ttagcttcat caagttggag catccataca gttttcattt caatatatat agtatgctat
    11521 ttgttccact tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt
    11581 ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttttaaa cggtgttcaa
    11641 tcaactgacc ctcggtattc gctcaaaaaa cgacaaaata tgaggagcta tttttgtttg
    11701 gctgttctta ttacttgatg caggacagct ttttcacgac ctctaattca cggtgttcaa
    11761 gcaagaaatc attgttcact gaaaagcgtt ggatgttctt tttcaaaggt gtctaagatc
    11821 tctcgtactt catcagtaga tttcgtattc atcaactgaa ctcgcaagtc atttgctcct
    11881 ggaaatcctt tgatatagat tttaaagaaa cgatgtagtc ctgtgatgga acgcgggatg
    11941 atttctgaat aatgatcttg cagatctagt tgcagacgaa gtagtccgat cagttctttt
    12001 tctgtatgtt ccctcggttc tttttcaaat gcataaggat tcttgaaaat cccccggcca
    12061 atcatgatgc catctacgcc atatttttcg gccaattctt ctcctgtctg gcgatctggg
    12121 atatctccgt tgatcgtgat cagtgtttgc ggagcgattt ggtcacgtaa agcaatgatt
    12181 ttagggatga cttcccaatg tgcatctact ttactcatct ctttacgcgt acgcaaatgg
    12241 acagaaagat tggctatatc ttgatgaagt aggtgactga tccattcttc catctcagac
    12301 atttccgtat agccgatacg cgtcttgacg ctgacaggca aaccgccagc tttagccgct
    12361 tcaatcaatt gtgccgctac ttctgggcga aggatcaagc cactgccttt tccacgttct
    12421 gctacattag ggactgggca gcccatgttg atatcgattc cttggaagcc catttctgcc
    12481 atagcaatgc tcatttctct aaaaaattct ggcttgtctc cccaaatatg ggcaaccatc
    12541 ggctgttcat cttctgtaaa ggtcaatctt cctcgtacac tgtcttttcc atctggatga
    12601 cagaaactgt ctgagtttgt aaactcagta aagaagacat ccggtgctgc tgcatgcttt
    12661 acgacatggc gaaatacaac atctgttaca tcttccattg gtgctaaaac aaaaaaaggc
    12721 tttggcagct ctttccaaaa attagtagtc atttatttgt tactctccat tctatcaagc
    12781 agtgaagtcg caatctttct caaaaacatt gtcattatac taggaaacaa aaaaagaagc
    12841 aaagtgaaca gcttaaaaga tgtttctgat tgagcaaaaa aagactgtga caaaaatctt
    12901 atcacaattc cttttccgga ataaatggtg ttcaaaagct tgcatctgcg gtaataagtc
    12961 caaccaaacg aaaaatagga ggagctattt ttgtttggct gttcttatta cttgatgcag
    13021 aacagctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
    13081 ctcaacaaac gacaaaatat gaggaactat tttcccgttt gtttctctaa tactcaggtc
    13141 aaaacgctga tttcacaacc tcttttctaa cttctcaatc ctcttccttt attgatcaaa
    13201 taccaagtaa cggcgtacaa aacaacgatg aatagcacaa gtacgatcat cgatgttgct
    13261 actggcacat cgattgttcc aaggaatccg taacggaaac cggagatcat ataaacgatc
    13321 ggattgactt tcgaaacagc ttgccaaaat ggcggcaaca tagagatggc atagaaaacg
    13381 ccacctagat aagttaacgg ctgcaagaca aatgtaggca cgatcgatac atcgtcataa
    13441 gactgagcaa aaatcccgtt gagcagtcct gctaacgaaa acaagattgc cgtcatcaat
    13501 aatgtaatga tgaccatgaa ccatgaatac acatgaagtg gtacaaagaa caaggaaatg
    13561 atcgttacga gagagccgac gagaatactg cgacccactc cgccaatcac aaagccccaa
    13621 atgatcacat gcgtaggaac aggcgctacc aagatttctt cgatgttttt ctggaatttt
    13681 tgagagaaga aggacgaaga aacgttggca tacgaactag tgatagcaga catcataatc
    13741 aaaccaggca cgatgaattc catatatgaa aaaccaccca tgtcaccgat ccgaccaccg
    13801 atcatttttc cgaaaataac aaagtacaag gatgtcgtga tcacaggcgg aaccagtgtt
    13861 tggacccaga tacgcaaata gcggttcgtt tccttgatag ctaaactttt taatgcagta
    13921 aaataaagat taaacatttt tctcctccgt atgattattt tcttctgtga ttttcaaaaa
    13981 cagttcttct aaacgattcg acttattcct catagaaaga actttgattc cttgtcttgt
    14041 cagctgatcg aagattccgt tcaccccttg atttcgttca acttcgacag cgattgtctg
    14101 ttcatcttca aatgtacaag cgtatccttc aatcacaggt ttggtctcat atggcgctaa
    14161 atcaaagata aatgtttcaa attgcagttt ggatagaagc tctttcatac tagtattctc
    14221 gatcagttca cctgattgga tgatcccgat gttgcgacaa agcatttcag cttcttctaa
    14281 ataatgcgtc gtcaaaatga tcgttgtacc attttcgttc aattctcgga gaaactccca
    14341 catctcgcgt ctcaattcaa tatcgactcc tgctgttggt tcatccaaga tcagcaaacg
    14401 tggttcatgc atcaatgccc tagcaatcat cagtcttcgc ttcattcccc cggaaagcat
    14461 acgtgcacgg acatttctct tttcccaaag attggactgc ttcaaatatt tttcgctgcg
    14521 tttcatcgct tctttgcgag agacaccgta gtagcctgct tgattcacga cgatttgctg
    14581 gaccgtttca aagggattga aattgaactc ttgcgggact aatccaatct gttgctttgc
    14641 tcggactaga tccgtatcca gatcataacc gaagacattc acttttccag atgttttatt
    14701 gactaaagaa gtaatgattc cgatcgttgt agacttccca gctccatttg gtcctaatag
    14761 cgcatagaaa tctccttctt tcacggaaag atcgattcct cgcaatgctt cgaccccact
    14821 tccataaacc tttttcaaat tatttatttc aagcgcattt gccatttatt tcctatctcc
    14881 ttatagacat gattctttat tgtatcatgg aatacagcag atgaaaaaac aacttggttc
    14941 cagcgaaaaa tcaggttgct gggctcagga aaatctccta atgaaagaca tcaatcggac
    15001 gaagtcagac aaccttttca gaaaaaacaa atcgaagata aaggattact attcatatgt
    15061 gcaacaaaag gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    15121 ataaacggcg gaagcaaaag ccaagatttt tgatccaata aatactttcg atcacttcgc
    15181 ctatcccaat acatgccact gccgcttttc atcaaataac gcggcttccg tttttgcggt
    15241 ttctactaaa gtctctgggt aatccaaaac tttcagcaag gcaatcgcat ttcgggtcct
    15301 tgaaggtcct tcttttaatt tgaaatcaaa actgattcct tttccttctg ttacttgttc
    15361 ttcaaagtgg acattctcac aataattctt taaaatctct gtcaattcga tatcatgtgt
    15421 ggcgacaaat gctaaagatg gatactccgc aagccaccga acgactgaag cggaagaagc
    15481 aatccgttcg atcgtatttg tcccttttaa aatctcatct acgaagcagt agcagcgttc
    15541 tttggaagcc acttggtcca acaaacgttt gatcgatttg atttctgaaa caaaataact
    15601 gtcgccttca aataaatcat cttcgattgc catggaagtc aacacatgtc caggttgcag
    15661 ggtaaactgt tctgcaatcg ctgtttggat cgtctgtgct agaatacagc tgatggctat
    15721 acttttgaca tacgtagatt ttcctgatgc attggaaccc gtgactaatg tattcttctg
    15781 ccagttgacc ggattcacta ccgcttcttt taacaatgga tgatagatat ctttcgctgt
    15841 cacaccgccc tcagtaaact ctggtcggca agtgatgggc atataagtac ggtaattcaa
    15901 tattgcagcg gctacttcta attctcccag caattcccac agcttgatgg cttcagttga
    15961 atgcttttct aatttagtga gaacgaaatg ataggagata aatggcagca tgaacatgat
    16021 gttgacatat tcaaaaatca actctgcttc actcccactc ttcatacgaa atgaaaaacc
    16081 taatttaggg atgtctctta atggtttagt ttgctgtttc agctcttctt gaagtggtgt
    16141 cggcagtttt gctagctgat tggcggctga aatcgttgaa accaaataac tcatgctatt
    16201 cagctctgtt tctagtgttt gtttcttcac tagataatag accgtattga atactaaccc
    16261 accgattgcc aaatagattc ctagtacttg tcctaaaaga agcaaaccca aacccaccag
    16321 cggaagcaag ccaagaaaaa taaataaacc tacgcgtcca atctttttac tttttccatc
    16381 cgctagatat tgttttgcaa aattatgatc tttttttcct aaacgagcga attgatattg
    16441 gattttttct ctcagttgag gattttcctg ataaaaagca atcagttttt ctaactgctg
    16501 atcttcacca aaatcaaagc tgcgtaaacg ttgatacaac gcttctgaac ctacactaga
    16561 ataggtactg ttgattcctt cgaatacttc gaacatatcc aggtcatacc aagtaagatc
    16621 atcgatttcg ctatcccatg aacggaactt cttctccgtg agccatgctt ccttcaagct
    16681 ttcttcttta tcaaatctcg gttgataagg aattttttcc catttgctac gaactgtttc
    16741 ctttaatttc aaacgtgaaa aaatgtctaa agcaactaat aaaataacag ctgcaataat
    16801 aattaaaacg ataaattctc tttgacccat atccctctcc gttcctttat ttgttagctt
    16861 tagtatagca ctgttgcgct atttgaaaag agggtaaaaa aacacctttc aaaataaaag
    16921 gtgttttttt cttgtatatt acatatttat ctctcattta tcaaaattga caaataatca
    16981 tagggaggaa ttgttgttaa ttcttgttta ttctccagtg cattgacaac tgtcccaagg
    17041 gaaaggatag cctcaggtac tattttttct tgttctgttg gattagctaa gcgaagaaca
    17101 aatccttgtt catgaaaata agaaggataa atcgatgata caattaagtc ttcaggtaat
    17161 tcaatcaatg aaaatttacg aggaacatca agttcatttt gtaatggcca aatcttgtta
    17221 tctaacctgt gaataaatac attcagtttc tgcttttgat aagaaataga ttgttctaac
    17281 cgtttttctg cctgttttgc cactttgaac tcattaaatc ttccttcttc tacattaaat
    17341 ccaaaagtga ctttatactc acctatctct tgagctagcg gtgtttccat catgacatgc
    17401 ccttcatttg tcgtatctcc tgaagctcgc ccaggtcgcc aagcaagatt aggttttcca
    17461 agttcccctg ttgttgaaaa taaggttatg attaattgat tttctatacg ttcatactct
    17521 tttaaaccat ctgcataaaa cgtaaaataa tgatcatttt ctgccacaga gacactctta
    17581 tcgaatattt ctaaattaac aggtttctct accatagttt ctgaccaatt cggaggaatg
    17641 acatcagggg tattttcaat aaatccgttt tgaatttgtg caattgattt ttcagattta
    17701 tctaaaacat taattgctaa gcgcatacga tgcgagtaaa cagtattatg aattttgagt
    17761 gtgccttcaa cgatttgact ttctcccaga ctgaacgata tttcataaga tatttcttcc
    17821 atttcgggtt tttcagataa acgatctttt aaatctttag gaagttgagc ttttccgtaa
    17881 acaactaatg tttcttgtaa agaatcttta tatactttag ctgtttctaa ttttagagat
    17941 agttctgtat ctccttctaa tggtgaataa tcatacgtat cgccatcatt tgctgaatcg
    18001 atcaaatgaa cgaaatcaag atattgtcta tctccaacta tcaaattcaa ttgcccatct
    18061 ttaaaagaaa gtttatattt attgttactt atctcagttt tgtctatctc gattgctgtt
    18121 tctaattttt cattattttt ctcaaattca acaacagtgt atcctagtgc tggcacattt
    18181 acatttattt ttatgtcaag ttcataataa gctggttcat caaaatattc gaaacctgta
    18241 gcaatcatcc gataagcatt tttccgttct ggaaaataaa cttctttttc aatgatatta
    18301 tagggactac ctttgaagtg tattttcttt gaatcagcta ctacatgcac gattttcgat
    18361 ccactataat tctcagtcgt tgtgttgaaa acaaggactt cattcgattt tagctttaat
    18421 gcatcagcca tccgttttac aataaggttt tcgattccgt cagctatctc attagcttct
    18481 ttgattcgat ggaaaatatc ttccgcaaca ttatctgaaa cacagccacc aatactatca
    18541 tgagcctggt tttgcaaaat ctttttccat aaccgacgaa gtaggccttt actgatatgg
    18601 ataccatttc tttctgcaat cagcatgagc ggctcaactc gttttagaat tttttgttcc
    18661 aatttaaaat tttctatttt catttttgtt cttactgagc caatagaacg atgaacacga
    18721 gagtaaaccg gctgacgaag ttcaccttta tattcaggta attctttttt gtttctgata
    18781 acttctacat aagaaggata atctgaaata atattgtgga atttactgta tttattcaac
    18841 ttctctacga cttgatcaaa atttaaaatc attgactttt gatctacacc cgaaggtact
    18901 aaaatatctt tgttttttcc ttgcatatga agcatttcaa ctgcttcatc caacttttct
    18961 tttgcaaatt caggaatatc tttaattgcg tctaaggtca tcattccagt catataacca
    19021 aatggaaaat tcatggcata aacaaattta tctcccaatc ctttccagat aaaataagga
    19081 gatggaacca tcgtttcaaa gtttaatcct cgccaacaca tgaaattatc gatttctact
    19141 tgatgcaaaa gtgtaggcaa attcgcattg aacccaaacg tgtcaggcaa ataccctacc
    19201 atcattggat ctccatattt aaggatcgta tcactgattc caatcattaa gtttcttaaa
    19261 attgattcag catccactaa taaggcatct gtttgtgcat accacggtcc cacgaatagt
    19321 tgtcctgttt tcatcagtcg ttggataacc ttaagctttt gcggattgat ctccaaatac
    19381 tcatcgacga ttgaagtttg tccatctaaa caaaagttta ctttagggtt cctttccaat
    19441 tcatctagaa cttcagtaaa gacctgatca gacaatacga tggcatcttc atttgtaaaa
    19501 taccattctc tgtcccagtg cgtgtgttga attatatgtg catttaccat atcagattct
    19561 cctactttcc ttcttctact gaatttactt gttgttcttt tttatacttg atggatttta
    19621 gtgcattgac cattaacgct gtaaccactc ccccaataac aattgatact aaatataaag
    19681 gtatattcgt aacaaaagga attgccataa aggttgaaag cattgtttct tgagtaacac
    19741 caaaaaatcc atttagacca catgctactg cagtgcccac cacgatgctt ggtattactc
    19801 gtaaaggatc tttcacggca aacgggatcg tcccttcagt aatccccaca aggcccataa
    19861 tcatagctgt ctttgcatta tctcgttccg attcatcata aagttttttg tttaaaattg
    19921 ttgctaaaaa cattccaaat ggcggaatag caattcctaa taacatggct gtactaggtc
    19981 caaagcttcc gcttgaaaaa gaagcaaccc caaaagcata tgcgatttta ttgataggtc
    20041 ctcccatatc aaacgccatc attcctccta aaatagccga catcaaagca gcatttgctc
    20101 ctgacatgcc ttctagccaa ttagtcagtc ctgttgtcat tgcttgaata ggcgtagcta
    20161 gtacatacca taatatgatt ccaacaacta atgtagtaac aaaaggaatc agcataattg
    20221 attttagact aataacttgc atcggtactt ttactttttt caatgcatta gctaaatagc
    20281 cacaagctaa tccagcaacc attcctccaa taaatcccat accaagattt tgacacatca
    20341 tcccagtgat aaaagctggt gctagaccgg gacggtcagc aattgaatat gcgatatagg
    20401 ctgaaatcat cggtacgata aactgtaatc ccgtctgacc aatcattcgc aggactcccc
    20461 agaatgtatc cgtctggtcc caaacacctg tgccaccaaa aatgactgca atagaagtta
    20521 gtaaccctgc tgctacgaca agcgggatca tataggaaac cccactttgg aaatgattta
    20581 gaatttctct tccttgtttc ttgaattcca ttagtaactc ccccacgata tattttttat
    20641 ttgttaatta atgcaattgc ttcatcaatc gtctcttcac catcttttac tacctctgag
    20701 gtccctactg ttaaaatcgg tttaccgtca aagcgacctc gttctgaaac atttgtatct
    20761 actgcaaata tgacaacatc tgcctcggaa acatccgaaa ttgaaagttt gttttctatc
    20821 cccatagccc cctgtgtttc tactttaata tctaaccctt tcttcttcgc aaacttttct
    20881 aaattagctt ttgccatata agtatgggca atccctgcga tacatgctgt tactgctact
    20941 atttttgtca tatgaaattc ctccttttaa aatgatagag cttttaccaa ttctttttta
    21001 tttctcgaat cttttattct tttaacaaac tcgtcatcca tcaatcttcc agataattct
    21061 gctaaaatac gtaaatgatt agtaccttta tcagtgtctt ttattgctaa caaaaagacg
    21121 agatcaactg gttgatcatc taatgcgccc cattcaacag accgctttaa cttggctaca
    21181 gccactgttg attcaattac tgctaggctt ttgccatgag gaatggcgat atttttccca
    21241 attcctgtgg tcatatggtt ttctctgtca aatacgtctt tttgaaattg tttgtggtca
    21301 gacacaaacc cattagtctc taacaatttc gataattttt cgactacact ttttttatca
    21361 gatgcattta aatctatcac tatattattt atattgattt tttcactgat catttataag
    21421 ccttctttca atatataaac taatttttca aacgacgaac tcgtttttaa atttgctaat
    21481 aactcatgat tcgaagcgat ttcattgaat gtttcataaa ttttattcaa ttcaggtttt
    21541 actttttcat taagtgcaaa aaagaaaaca gaatgaacaa gtgtcccatc ccaatcaatt
    21601 ccttctggat taacgtagac acagattact ggctttttta taaaatctac ttcgatatgc
    21661 ggcatagcaa aatcattcat cgctgttgaa gatagttttt ctcttttgat tgctccctct
    21721 agtatccctt ctttcgcgta attttctttt accaattttt tggtaattat atttaaaacg
    21781 ttcacttgat tatcctttat ttggtgggta aaaaagcaaa atcgtttatc taataaattt
    21841 agaaaagcat cttctgtttg ttcatttaac tgtagatact gttttacttt tctgacatct
    21901 gcccctaaca tcaaaggact aacaacaata acaggaaaat ttgtattttc aatcggaatc
    21961 gtgcttataa caagttcatc agtgactttt ctatcttgta gttcattgat gcttagtaca
    22021 tcttctatct caaccatatc tgcaaaaccg ttatttattc tttgctctaa taattttgat
    22081 gttccatatc cgctactaca aactaatatc actttcgttt tgtcaggttt gtaacgatcc
    22141 aaaagtgatt gaatatggag tgttacgtaa gcgatttcat cttcattaaa atgtatacag
    22201 aatctctctt cgatttcctc taataaatct acacttatca taaatgacct tttaaaagaa
    22261 tatttgattt tgtccgtata agggttatag atatttactc c
//
LOCUS       DASJUF010000045        21985 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJUF010000045 DASJUF010000000
VERSION     DASJUF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.045"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..328)
                     /locus_tag="U4B62_001502"
     CDS             complement(26..328)
                     /locus_tag="U4B62_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2141666.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="U4B62_001503"
     CDS             complement(353..2446)
                     /locus_tag="U4B62_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2141667.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="U4B62_001504"
     CDS             complement(2935..3699)
                     /locus_tag="U4B62_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN2141668.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="U4B62_001505"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="U4B62_001505"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN2141669.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="U4B62_001506"
     CDS             complement(4704..5780)
                     /locus_tag="U4B62_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN2141670.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="U4B62_001507"
     CDS             complement(5804..6397)
                     /locus_tag="U4B62_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN2141671.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="U4B62_001508"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="U4B62_001508"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN2141672.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="U4B62_001509"
     CDS             complement(8067..8366)
                     /locus_tag="U4B62_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN2141673.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="U4B62_001510"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="U4B62_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN2141674.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="U4B62_001511"
     CDS             complement(10121..11032)
                     /locus_tag="U4B62_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2141675.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="U4B62_001512"
     CDS             11152..12009
                     /locus_tag="U4B62_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2141676.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="U4B62_001513"
     CDS             complement(12196..12558)
                     /locus_tag="U4B62_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN2141677.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="U4B62_001514"
     CDS             12904..14343
                     /locus_tag="U4B62_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141678.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="U4B62_001515"
     CDS             complement(14350..16902)
                     /locus_tag="U4B62_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN2141679.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="U4B62_001516"
     CDS             complement(17016..18347)
                     /locus_tag="U4B62_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2141680.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="U4B62_001517"
     CDS             complement(18313..19791)
                     /locus_tag="U4B62_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2141681.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="U4B62_001518"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="U4B62_001518"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2141682.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         6400 a         4692 c         3769 g         7124 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatc
//
LOCUS       DASJUF010000046        21736 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJUF010000046 DASJUF010000000
VERSION     DASJUF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.046"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(196..1095)
                     /locus_tag="U4B62_001519"
     CDS             complement(196..1095)
                     /locus_tag="U4B62_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN2141683.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1105..1461)
                     /locus_tag="U4B62_001520"
     CDS             complement(1105..1461)
                     /locus_tag="U4B62_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2141684.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1816..2112
                     /locus_tag="U4B62_001521"
     CDS             1816..2112
                     /locus_tag="U4B62_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN2141685.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2756..3709)
                     /locus_tag="U4B62_001522"
     CDS             complement(2756..3709)
                     /locus_tag="U4B62_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2141686.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3732..4487)
                     /locus_tag="U4B62_001523"
     CDS             complement(3732..4487)
                     /locus_tag="U4B62_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2141687.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4484..5443)
                     /locus_tag="U4B62_001524"
     CDS             complement(4484..5443)
                     /locus_tag="U4B62_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2141688.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5436..6386)
                     /locus_tag="U4B62_001525"
     CDS             complement(5436..6386)
                     /locus_tag="U4B62_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2141689.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6536..6988
                     /locus_tag="U4B62_001526"
     CDS             6536..6988
                     /locus_tag="U4B62_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN2141690.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7077..9344)
                     /gene="gshAB"
                     /locus_tag="U4B62_001527"
     CDS             complement(7077..9344)
                     /gene="gshAB"
                     /locus_tag="U4B62_001527"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN2141691.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9531..10529)
                     /gene="ftsY"
                     /locus_tag="U4B62_001528"
     CDS             complement(9531..10529)
                     /gene="ftsY"
                     /locus_tag="U4B62_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN2141692.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10540..11349)
                     /locus_tag="U4B62_001529"
     CDS             complement(10540..11349)
                     /locus_tag="U4B62_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2141693.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11358..14939)
                     /gene="smc"
                     /locus_tag="U4B62_001530"
     CDS             complement(11358..14939)
                     /gene="smc"
                     /locus_tag="U4B62_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN2141694.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14959..15645)
                     /locus_tag="U4B62_001531"
     CDS             complement(14959..15645)
                     /locus_tag="U4B62_001531"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN2141695.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15993..17774)
                     /locus_tag="U4B62_001532"
     CDS             complement(15993..17774)
                     /locus_tag="U4B62_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2141696.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17800..18714)
                     /locus_tag="U4B62_001533"
     CDS             complement(17800..18714)
                     /locus_tag="U4B62_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141697.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18741..19703)
                     /locus_tag="U4B62_001534"
     CDS             complement(18741..19703)
                     /locus_tag="U4B62_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141698.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19703..20647)
                     /locus_tag="U4B62_001535"
     CDS             complement(19703..20647)
                     /locus_tag="U4B62_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141699.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20648..21652)
                     /locus_tag="U4B62_001536"
     CDS             complement(20648..21652)
                     /locus_tag="U4B62_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141700.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6075 a         4642 c         3848 g         7171 t
ORIGIN      
        1 ttttgtgatt atcgttcaaa aaaagaatac acgcttgcat ctagtactct gcgagttaca
       61 aatcatccga tcaaataaac attcgctgat tattttccgc aaaaaaagca gcatatccac
      121 gataaatgga tctactgctc ttacttatcc ttatgtttta caaggtattt tcttttacac
      181 attactaatg ctgtctcaat acggaacgat tgtcatcgct tgttcaaatt cctcttgcaa
      241 atgttgtgtt tgataggcct gaaaagctgg gtgatgggca cgcaagagga cacgtaattt
      301 cataggatca tagctgtcta gcaactgttc tttcaagaca aaatctcggg tcagctgcca
      361 ttcataataa taaccaaaag gtctgcagct gtaaatcgtg tgaccgatgg atttatcttc
      421 aaagcgacga tgcgtatggc caaaaactac ttgtttgata tttgaataac ggtcaagtaa
      481 ttctccgaat gattctgatc ctaaaaatgc atttaatttg ttccaacgct cataaggagc
      541 agattgatac atgataaatg cccgtttagg aacgaaatga gtggataaaa tcacctcaag
      601 tttttttgct tccagctggt ctagcaaatt ttttgcagct gcaagcattt ctgcattgac
      661 ctcgagatct gttttcccac gctggatgaa ccgatcatac caatacaaac gcttcattcg
      721 tacgattgcc tcgtaatctt tcaattcgga aaactgatag tcgtaccagc cattcatccc
      781 taaaagaacg gtctgctcat ttaaagcaat ataccgttca tttaaaaaat gtgggtctgg
      841 aaaatggtcc atcatctgtt cattgggcac atctgccaac tcgtgattgc cgaaatgaaa
      901 tgtcgtagat aatcctttat tgacgaaaaa ttcgacaaca gctaggcaac ggtctacttt
      961 attagccgta tctccggcta gatgaagccg agtgatcttg tgcttttgca aaacttgcca
     1021 aagctgcatc agctctgatt cgccgaattt attgatgtcc acatgtaggt cgctgatgat
     1081 cgccaatttt cccattttat ccctctagtc ttttcgaaca acttgcaagt tgccttttct
     1141 caagccgctt ggtcctttta attgtaaact attataagtt ggttctaaag caagtgtgga
     1201 tggatcatcc atgtaaagta ttgcactgtc tttgattttg attggtccaa ctggtgtatc
     1261 agccactaca gaataaagac ttagatctgt ttgtgtatct tttttgacaa tgatcaatcg
     1321 aaatgccgta tccaattggc aactgacttg gctttctgca aatggaccgt caccgttttc
     1381 gatatctaat aaaagttgat cgtctgcttc taatttattc ttcaatactt cttgtgcttc
     1441 ttttgtcact tctaatttca tctgtatcac ctcatgtcat agaataatca ctttattcat
     1501 cagatgcaac taatttgtct ggttctgact gttctcaatt tattggaaag taagtcattc
     1561 gtttattatg atttctctta tcacaaaaga gacaactttc ccttttcttc gttcagtttc
     1621 ttgaacggaa aaagacccaa aaagcaacct gttatttgtt taaccttatt cctttcttaa
     1681 ataacaaaaa atactataaa aaataaaaaa cctttaacaa ctattggaaa aagaattact
     1741 ttttgttcac ggattattaa caactcttcg gtagagtaaa cttatcaaat aaaagacttc
     1801 tttggaggaa tcactatgaa ggaatatata aaaaacatct attttatcga agaaacacaa
     1861 aatatcgagg gttcttatat tgaagtaaaa acactctttg tcaatgaaga taaaaccaaa
     1921 gcattggata tttataaaaa attagcttca aaaaaaacga atagttttgg attgatatta
     1981 agcgagtata aaatcaaagc agaagagagt tatttctatc aattactaaa acggtggagt
     2041 aagcttcccg ccgattttta tcgaaaaatg cagatcatca actatcaacc gcttgctgag
     2101 acacatgcct aaatagaggt tgtgaaatca gtgctttgag ctaataccga aggtcagctg
     2161 attgaacatt gtttagaaga gattgtgaca actgcgattt gctttgagta ataagaacgc
     2221 tggacagaaa atagcttctc atattttcta gtctagtggg cttattgccg agaagcagca
     2281 gatggaacat cgtttagaag agattgtgac aactgcgatt tgttttgagt aataagaacg
     2341 ctggacagaa aatagcttct catattttct agtctagtgg acttattgcc gagaagcagc
     2401 agatggaaca tcgtttaaag aagagatcgg tacaaaataa aaatatctaa atttaagttt
     2461 tatctaagtt tcgctttgta cctttgattc aaatcaaagg aggcgatctt gatgctgaaa
     2521 aaatggatca cactattttt tgccactccc cattctagaa tcaacccgta tgatgtagat
     2581 aatgaattca cgttgaaaaa agatttctca aataaataaa aaccatccgt aaaatcatca
     2641 atacgatttt acggatggtt tttgataact tctttgagga taggctcttg ctatttaaga
     2701 atgtaagaaa cattattgtt acatagtatg agagtttaat cctctgttat ttatgctatt
     2761 taaatgcttg attcacatct tcgatcatca gtttcattga ttctaaaccg cccccgctta
     2821 aataccagac atctggttca agggagatga tctgctgatt tttaccagca ttcgtttgtg
     2881 ctaccagttc attggcagaa atatcatctt tgctgtcgtc tccaccaata gcttttgttc
     2941 tgtctactac aaataagata tccggatttt tttccagcac atattcataa gaaacacttt
     3001 gaccgtgagt ggatgcttcg atttgatcat ctgcttgttc aaagccaaat aaatcatgga
     3061 tgaaaccgaa tctagaacca gatccgtagg ctgataattg cccttcattc acaagtgtga
     3121 ctagtgcttt tttgtcagta gcttctgctt tctttttcgt ctgatcgatt gcactagata
     3181 attcctctaa ttgagtttta gcttcttctt gcttaccaaa gatctcccct aaagcagtga
     3241 cattttgctg gaaagattcc catggatttt tcgcatctaa agaaaggaaa atggtcggag
     3301 caatttgact tagatctttt tgaaaatctc tttgtcggcc agaaatgatg attagatctg
     3361 gctgaagctg attaattttt tctaagtctg gttcttttat tccacctgct gattctactt
     3421 ttttatagga tgaaagatac tcaggaaggc tgcttgtagc tgctccaact accttatctc
     3481 caacaccaag agcatccatc gtatctaaag aaccattgtc gaatacgacg actttttctg
     3541 gattatttgg cacctcgatt tggtctccat tactatctgt tatcgtcaat gtagtttcag
     3601 tttgtgtgct gctttttttt gtgtctgcct gactggtgtt gttgctattt ccacatgctg
     3661 ctaaaccaaa tgatgctaga atcaagatac ctactactaa ttttttcatc tcttctctcc
     3721 taatgttagt tttagaaata aagacagaat ctgcggcctt caatctcaca aatccgtatg
     3781 tccatttcgt ataattcatc caatacatct ttttttatca tttcttgggt cgttccttgc
     3841 gtaaataact gtccatcttt cattgcaaca atttcgtctg cataacttgc agcaaagttg
     3901 atatcatgaa gaacaataac aattgttttc ccgatttctt gtactaaatt ttttaaaatc
     3961 tgcatgattt gaaccgcaaa attcatatct agattgttca aaggttcatc cagtaaaata
     4021 taatccgtgt cttgcgctag caccattgca atatacgcac gttggagttg accgcctgaa
     4081 agagtgtcaa tcgattgatc cgctatttct tctaacccaa tgtgttccag tgattcctga
     4141 acgatttcct cgtcttttgt cgtcaatttt cctttcgtat aaggaaatcg tccaaattct
     4201 actaattctt tcacggtgat ttggacattt atttcattcg attgtcgtag gatcgataat
     4261 tttttggcta attcattgga tttccaagcc tttacttcgt tatggtctaa atagatttca
     4321 cctgtatctt ttggtatcaa acgactgatc atcgacaaca ccgttgactt tccagcacca
     4381 tttggaccga taaaagcagt caacttcttt tcaggaatcg ttaactgtac atctttaacc
     4441 actgattttc catggtacag tttattgacc ttttgtaaat caatcatagg atactcttcc
     4501 tttcgcgaat caattttgtg acaaaataaa ttcctccgac aaattcaacg acgacactca
     4561 ctgttgtatt ccagccgaaa atctgctcta ctgcgagctg tcctcctgtc agaaacagca
     4621 tagctaccag aaagcttccg ataaatagct gaccatgacg ataagtagaa aagacacgat
     4681 aactgatatt tgcgacaata aaccctaaaa aagtaactgg acccacaaga gccgttgatg
     4741 tccctgtcaa aagactgaca caacataaaa tcaaaaattc aaaacgatct gtgtctatcc
     4801 cgaggtttac tgcttgttca tttccgagat ggaaaacgtc tagcacatgt cgatatcgaa
     4861 ataacaggta ccagcagccg ataatgatca aagaagcagg caaaagatac tgactctgga
     4921 tattgccaaa gcttgcaaat aatctgcctt gaagcaaatc atattcattc ggatccatga
     4981 tgacttgtaa aaaggtactg acactaccaa aaagcgtacc taaaatcatc cctgacatca
     5041 gtaataaaaa aagattcgct ctcatttttt tcaaaagaaa gaaaaataac aatagactca
     5101 gcccgaccat cagcccaacg ttcaataaga aaaaaactgt acctgtctgc tgaagcatcg
     5161 tcactcctcc gataaagaag aaaaggaccg tttggacaag gacatataac gactcaagtc
     5221 ccaatactga aggagtcaaa aactggttat gcgtaattgt ctgaaagctg attgtggcac
     5281 tgctgacagc tagcgagaca aggataaatg caaaaatttt cttactcctc aatgcacatg
     5341 caaaatgcca gtttccatat gtatggatcg tcaagtacat tcctgtaaca aataaaacaa
     5401 gaacaagcat accaataaaa atagatggct tttttttaag catatcgtcg tccccttatc
     5461 agaagataaa ggaagatagc cccaccgatg attccaacga tcacactgac tggtatttca
     5521 taaggaaaaa tcaacacgcg agcgacaaga tcgcaaaata ctaagaacaa actacctgct
     5581 aaagcagtta cgccaattgt tttgtgcact tgatctccat atattctaga gacaagattc
     5641 ggaatgatca ctccgataaa aggaatgctt cctaccatga tcaacatgac tgcactagcc
     5701 aatgatacta agccaatgcc taaaaactgc atcttctggt aatctattcc cagatttact
     5761 gcaaacgatt cgcccagacc aatgatcgta aatcggtaag caaatagcca aaccaagagc
     5821 aacaatggaa caacagcata caataactca taatttcctc gtatcacagt cgcaaaattc
     5881 ccttgaagcc atgaagtcat attttgtacc aactggaatt gataagcaaa aaaagtagca
     5941 actgaaccaa tgatattccc taacatgact ccaatcaacg gaagcatcaa ttgatttttc
     6001 tggaggaaca atcgcgtaag ggataaaaaa agcaatgttc ctgcaaaagc aaagcaaaat
     6061 gctgtcaagg atcggacaat caaggatgca ccaggtagga aaatcatagc cactaaaata
     6121 cctaaacgcg cactatccat cgtgcccgca gtcgttggtg aaacaaattt attttgggtc
     6181 aaatgttgca tgatcaaccc acaaatacta ctggtcgctc ccacaaggat caaacaaatc
     6241 gttcgtggca aacgagtcac tagcaagatt tgtaagcttt gcccatctaa ctggagcagt
     6301 tctgacaatt gtacattcga tactccgatg aacaatgaaa tactacttaa aataactaat
     6361 aatgctacta gtcgtccgta gcgcatccaa tccacctttc taattgagaa tgattttcat
     6421 tcttatttta agtgaatcgt tgaacaaaag caaccgcttt ttcatatgtt ttaggtatta
     6481 ttgggaatat gctataatcg taagaaagac aaagagaaga aggagggtat tttttatggc
     6541 acaagatgat gtaaaaagcc atttggataa gggactttat ggtacacctt tagtgaaccc
     6601 tgaagaacag cataaatatt tagggacatt cagggaacgc tgctatctta gtatgactgt
     6661 tgctgaaatg atccaaccaa aaaacaaaga aaacttttta aaagaactca aaaaacatcc
     6721 agatgcttcc atactcttga atggggctct acccctttct gtccaaaacc aatatatcca
     6781 gctagcaacg aaaaacaata gccgtttcac tgtagtcaac gattacgtca ctgcaaatcc
     6841 tgaggaattc ggattgctct taacagcaaa agaagcagta aatgaacctg tgattgatat
     6901 cgaaaaaaaa tacccagaag aaaactcttc gccagctgaa aaagaaaaac caaaaaagaa
     6961 aaaaagcttc tgggatcgtt tattttaaaa cgaaaaaaca cataaatttt tacatgcaaa
     7021 aaaagctttt gatcagattg acatcaaata ggacaatctg tcaaaagctt tttcttttaa
     7081 aatacttcag gatataacaa tttcaataca ttcatcgtca atcttctgcc ttttcctgca
     7141 aaaggataaa catgcatatg catggctgga ttgaaatttg cttcgatgat cccatatgct
     7201 tttttatcag ttgtaggatc gatttcttta tctggtataa tcaaatcgat accactaatc
     7261 ttagctccta gtgcttctac cgcagaaacg gcgatttttt tgtaagcttc agaaaattca
     7321 tcagtcatat cgatcgaatc accacctgta ctgatatttg agttctccct tagataaaca
     7381 atttgattgg cttggggaac tgattcgatc gtcaaccctt gttctttcaa catcaattgt
     7441 tctaattcac ctaattggat caattctaaa ggtgcacgat gattcgttcc acgcaaaggg
     7501 tcactatttt tttcttcaac aagttctttc actgttcgga tactgtcacc aataacatta
     7561 gcagggactc gcaacatgat ggcttggact tctccgtcga tcacaaagaa acgatattcc
     7621 gttcccggca taaattcttc tactaaaacc gaactgtctt cacgaaaagc aatggctaaa
     7681 cctgcttgat aatcttccag actagctcct tccttgaaaa tcgtgatacc taacccgtaa
     7741 ttggttgatt taggtttgat cacaaatgcc tgctcggcaa atctcggata agcttttacc
     7801 gcttcttcca tggatgaaaa ttctgcgcct cctggtaccc ggaacccagc ttcttttaag
     7861 acttttttcg ttactgtttt gttttccatg ataagtggga cgatatagct gtccttactt
     7921 gtcatgtttg catttttcac atattctaca tggtcttgga attgcagacg caaaaattga
     7981 tccgactcat ctaatacttt gacttggaca cctttttgga tggcatcgaa cataaatatc
     8041 tgagtagaaa gttccatttc gcgaaaaccg gccaactgat agggacgttc atggctttct
     8101 ttataatatt gatgtcccaa tgccacagca acttcttgct gactcgatag ctgtgcttct
     8161 tgatagagtt tcgctgctaa cgtttgttca ggatgatcca tttgagtcaa tgcttcctct
     8221 actaaataaa gagaatccaa ccaatccaat tcaaccagca tttgcttcat tccttcaaga
     8281 atttctctac cttcttgatg aaattctgtt tgatctgatg gttgttctag tgctactttg
     8341 ttatttcgag tcgtaccttc tgctacccat tggtcacagt cttctttttc ttctgtccat
     8401 aacatgtaca tcaggaataa ttgtaagaac cgaacttgct ctggattgat acccaatctt
     8461 gcataaggat tcaaatcgat attccgcaat tcaatataac gaacacctgt tgttgctaaa
     8521 tctgcgactt tctttccgcc tcggaaacga agcggtgtat aaaattcttt ctcctccgaa
     8581 agtttccctt cttcgatcat tcgctcgata tctgctaaat actgtttcat agaagcatag
     8641 gaaactttca catttggatg attcgtatag cccagcgcac tgttgcgaat actgcgaaca
     8701 ggttcttgcg gatgtgattc atctaaaaag tagttcttct cactcattgg tgaagcaccg
     8761 aataaataag tgatcatcca gcgatagcgc ataaagtttc tcgctgtttt caaataaagt
     8821 tctgttttga attcggaaaa atcttgaaat tcttcctgat ggctgaacaa tgttcttagc
     8881 aactcatcgc caaattcaaa attgaaatga ataccgctta ccatctgttt tcgcttcccg
     8941 tattcttttg ctaaataacg tcgatacaag acatcttcga aattttttaa ttttgcaata
     9001 atgatctctt cgtctttttc cggtaaggca ggtggcatac ttaatggcca gatcatctct
     9061 tctttcggta tagaacgagc agtcacatca taaacagcag cgagatactg aaataattcg
     9121 ggaatagaat cggtaacagg ggtgatcatc tctgtttgtg tttcgctaaa atctgtttgg
     9181 atataggggt gataggatcg atcgccaaag attgctggat gatcggtttt tgctagattt
     9241 cctgcaagat caacccgctg tccttcccgt tctataccaa atcgagcttg gtcgataaaa
     9301 gggcgcgcat tgacatgcaa taataattgc ttaaaattca tcattaagtg acctccaatt
     9361 catatgaatc taattataat caaaaagtct aagaaaaaga acgaaattgt ttgagaaccg
     9421 cttacaaata agtataaaaa tgaaggcatc aaaaacacag ctatgttaaa aacaatcatt
     9481 caaacaagga gctgcaactt ttgtcacagc tcctgctttt tagacatggg ttattcttct
     9541 tttagtaatc ctttgaacaa tccaacgaca aaatcatttg gatcaaacgg ctccaagtca
     9601 tcgattcctt caccaagacc gaccaatttc actggtaaat gcaattcatt acggatagca
     9661 agtacgatac cgccctttgc tgtaccatct aattttgtca aaacaagacc ggtcacatct
     9721 gttgtttctt tgaattgttt tgcttgaacc atcgcgtttt gaccagtggt tgcatctaat
     9781 acaagaagtg tttcttgggg tgcttctggt aattcccgct ggattactcg ttttattttt
     9841 tctaattcgt tcatcaagtt cactttattt tgcagtcggc ctgcagtatc aactagtaaa
     9901 acatctgcct gctctgtctt tgctctttcc aaagcatcga atacgacagc tgctggatcg
     9961 cctcctgcat tgccacgaac aacttcaaca ccagcgcgtt cgccccagac aactaactgg
    10021 tcgattgctc cagcgcggaa ggtatctgct gctgccatca atactttttt accttcgttt
    10081 ttgaactgat gggcaagttt accaatactt gttgttttcc ctacaccatt aacaccgaca
    10141 aacaaaataa ctgttagtcc gtcttcttgg atatttagtt catttacttc attgattcct
    10201 tccgcttcat agagatccac aagtttctcg atgatcgcat tttggacttc tgccgatttt
    10261 ttcgcatttc tgagtttcac ttcttgacgt aatgcttcgg tgattcgtat cgctgtttca
    10321 aaacctacat ctgcaccgat aagtgtttct tctaattctt caaaaaagtc ttcatcgact
    10381 gttcgaaaat tggcaaacaa ctcattcatc cgttcaccaa atgttttacg cgtcttttct
    10441 aaacccttgt cgtacttctc ctgtacttca acttcttctg ttttctcttc gccagtaaaa
    10501 gcacgcttga ttttatcaaa aaatcccatt tgactccccc tactctaata caaatttttg
    10561 gatcactttg gctacgccat tttcttcatt gctggctgtc acgaaatctg ctgcttcttt
    10621 gacgaaaggc acagcattct ccatcgctac gcctagtcca gcatattgga tcatcggtaa
    10681 atcattttcc tcatcaccga tcgccatgat ttcttcttgt ttcaaaccaa gatcttttgc
    10741 caataaagaa ataccataag cttttgtaac accttttggc ataaattcca aaagcatact
    10801 tcttgttttg atgatctcat acatttcatg gaattctgct ggtatttctt tgattttagg
    10861 atctaattct tcttggtcat aagcgattac tgccttatta agcacaaatt catctgtaat
    10921 atcgcttata tccgctggtt ggaattgcag cagcttattc aacacattgt acaaggattg
    10981 cttccttggg gcactaggca gctgtaaaac cacattgtca gacaaaacat ctagtggcag
    11041 atccaattgc tgagccagtt tgtataagcg ttggatatct gatacttcca tcactttctt
    11101 ttccatgatt tcaccagtat catttttttg taccaaaccg ccgttgaaag tgatgctgta
    11161 gtcaccttca tctaccagtc ctaattcttg cagataatgc gccatcgcag ctaaggggcg
    11221 tcctgtacat agaacgatct tgaccccttg ctgttttgct tgtgctaatg cttgtttatt
    11281 ctcatctgaa atcagctttt gttcatttaa taatgtaccg tccaaatcga ttgctactaa
    11341 tttgatcatt ttcgttccta cactttcgtt tcgttgattt tcgcctcttc attgatatct
    11401 tccatccgta cagaaacgat ttttgataca ccggattcct gcatcgtcac tccgtacagt
    11461 acatctgctg cgaccatcgt tcctttgcgg tgtgtgacta cgataaattg tgtgtcatct
    11521 tggaaatcgc tcaagtaacg accaaaacgt ttcacgttgg cttcatctaa cgctgcttct
    11581 acctcatcta agatacaaaa cggaacagga cagacacgga taatggaaaa gagcaacgcg
    11641 atggctgtaa gtgctcgttc tcctccagaa agcaaactta gactttgcag ttttttgcca
    11701 ggaggttgta cttcaatctc aatacctgtt ttcagcaaat cagttggttc cgtcagtact
    11761 aattcagctt taccgccgcc aaacatattt ggaaagacaa ttttgaattc ttgacggata
    11821 gcttcaaata cttctttgaa tcttgtccgc acttcatcat ccatctcatc catcgtttca
    11881 aacaattggt ttttcgctgc taacagatca tctcgctgtg ttgctaagaa tgtatgtctt
    11941 tcactgactt gttcgtattg ttcgattgaa tttaagttta ctggtcccag tttttcaatc
    12001 tgttctttta aggatgaaac ctttgttcta ctgtcttcga tatcggttgt ttcttgatag
    12061 tcttgggaag ctttctcaaa tgtcagctga tattctgact gcagataggt caaatgatta
    12121 tctaaaatca tttcagctcg gtctttttgg acttctaatt tggattgttc cgacagtcgt
    12181 gctttttgct cacgattacg ctctgccaag actgcttcaa gcttatcgat ctccttttgc
    12241 cttctatctc gctgctcttt ggcttttgcc aactcggctt ttaatgtttc tcgctgagca
    12301 gataattcgt taatttgttt ttccaaactt tcttctgtga cttcatgatc agaaaagtct
    12361 gctgtcaatg tcgccaactg cttttctatc gcttcttgac gttcggttgc ctctgctttt
    12421 tggactcggg caccgcggag ctggatctgc agatgattaa actgctcttt caagacagca
    12481 agatctgctt gttcttgtgc tttttgactt tgtacttgtg ctcttctggc ttccatctgg
    12541 tcgctttctt gacttaacga tttgatttct tcgtcgattt tttgtcgttg cgattcaaga
    12601 tctgtttgtt tgtctttcaa ttcgttcgtt tgcttttcgt aatcttcgat aaactgctgc
    12661 agttcacgtg tctcaaaagt tgagatttgc ttttcttttt caaaacgttc aagttcattt
    12721 atgatgtttt gtagctgatt tgtcgcttct tgttcttcaa aacgtaattg ttcgcctcgt
    12781 gtacgcaaaa cttcttgttc ttcggctaaa cgagctgttt cttgttgcag ctcctgaact
    12841 tttttttctt gtgcttggag ttgtttatct gcttcttcaa attcactagt caactgtttc
    12901 aattcttgat tttgtacaaa caaactgcct tggtttcctc gtttattcgc tccacctgtc
    12961 atggatcctc cggcattcat cacatcacct tctaaggaaa ccactcgata ttgatagcgg
    13021 atcgtttgcg cgattgcatt ggcactagtt aagtctttgg ctaaaaggat cgttcctagc
    13081 aggttttgaa ccacagtttg gatttgatcg ggataactga cttgttcact ggcaatccct
    13141 ataaaccctt ctacagctgc tgcttgtgtc aatatatgag ccggtaattg tcttggtttg
    13201 attgtcgtca gtggaaggaa tgttgctctt cctcctcttt gctgtttcaa atacgtgatg
    13261 gcttgccgag catctttttc attttcaacg atgacatgct gggctgcccc acctaaagct
    13321 gcttcgatag ctaacgtgaa atctgcagga acatcgatca gttcagccac tgctccgaca
    13381 atacccgaaa gctgctgttt atgctgcaag accaatcgaa ccccttggta aaaaccaaaa
    13441 tagttttctt ggatttcttg aaggcttttc ttacgagcac gtagctgctg gacttgattc
    13501 attaattgat acattttagg ctgttctttt gctaatgctt cttggaactt tttcccgttt
    13561 tgttggatca tttctaactt ctcttttgtt tcagttaaag atgattttag ctgcgcctgc
    13621 ttttgtgtca attcttcttt ttgtaagatc aatcggtcta ctgatgcttc tacttccgat
    13681 tgcttggcta acgtctgctt gctttttgct gtttcttgta tatattgacg ttctagatac
    13741 ttcagttcgt ttcctacagc tgcttgttct tgcatcaaat caacatattg atctcttaat
    13801 tctgccaata attctttcgt tgatttttgg tatttttcta attcttgctg tgtttttatg
    13861 agatttgctt cagctttttg gatctctgtt tctttttctg ctgctgcttt catcaagctt
    13921 tcttgaagct tttcaaaatg tttaaccttt ttttgtgctt ctgctaatga agtttgatat
    13981 tcttgcgagc ttttttgtgt atgctttgtc cgctcctgca aaacatcttt ttgtccttct
    14041 gtctgcttca gtttttccga aagatctaat aatacttgct gatttttttc gatcagacga
    14101 tcggcttgcg cattttcttt acgctgcttc gccaaaatgg attcttgttc ttggatcgac
    14161 tcagatagtt tgcctaattc tagattgaat ttcgcaagct gtgcttgttt gttgtcccaa
    14221 tctttttttg ctgtcttgat ttcagcaacc atcaaagaga catcggtttg agtcagtgtt
    14281 tcttttagcc gtaaaaactc ctttgctgcc tcactctggg cggccaaagg agttaactgt
    14341 tcttccaatt cgtggatgat gtcttggacg cggctgaggt tgtcttctgt ttcaaaaagc
    14401 ttctgttctg ctttcttttt ccgctgcttg tatttcaaga caccagctgc ttcttcaaag
    14461 atcccacgac ggtcttccgg tttactactg aaaatcgctt ctacttttcc ttgagaaatg
    14521 attgagaacg attcttttcc taatccagaa tctaagaaca attcttggat atcttttaat
    14581 ctgcagcttt gtttattgat gaaaaactcg ctttccccag tccgtcgata acggcgggtt
    14641 acactgatct cattgtactc caatggcaga taatgatcac tattgtctaa aatcactgtg
    14701 acttctgcaa tattcagctg cttacgggta tcggaaccgg caaagatgat atccggcatt
    14761 tttccgcctc gcagactttt agcagattgt tctcctaata cccagcggat agcctctgta
    14821 atattgcttt ttccgctgcc atttggtcca accactgctg tgacgctatt ttcaaaatca
    14881 atgactgtct tgtctgcaaa tgatttaaaa ccggctattt caattcgttt caaatacaaa
    14941 gctttccctc acttactttt acagtgctgc caatgcattt tccgccgcag cttgttctgc
    15001 taattttttc gatttccctt gtccagtgcc aatcaattga tcatctacat aaacttcgat
    15061 ccaaaatact cgttcatgag ctggaccttc ttcattgatc aatcggtatt cgattgatac
    15121 atcacctgat ttttgcaaga cttcttgtag ttttgtttta tgatccatct catgtgaaaa
    15181 agcacctgct ttgactttag gaaaaatcac tttttcgatg aatgcatggg tcgtgtcgaa
    15241 tccctggtca agatataacg cgcctaaaaa agcttcgaat aggtcgcaaa gcaatgctgg
    15301 tcgcgtacgt cctcctgaat tctcttctcc cttgcccaac aaaacatatt gatcaaagtg
    15361 acattctttg gcaaatttcg acaagctgtc ttcgcgaacg atcgcagctc gtgtttttgt
    15421 caattttcct tctggcaaat gaggatataa acggaataaa tactgagaaa ccatcagttc
    15481 taaaacggca tctcctaaaa attcaagtcg ctcattatcc gataattgca gattgcgatg
    15541 ctcattcaca taggatgaat gggtaaaagc ttgttcaagc aaatttagat cgtgaaaaac
    15601 gatgttatag ttttctttta gttcttttgt cagctggttg tccattttcc acattccttt
    15661 tatggatatt ttttcgatac ttccttatta tactggtttt ttgtcaaatt tgaaatggtt
    15721 cggtcaaaag gtaagtaaag ctttaataat cctagtgatt gacgagaagt tgtgaaaaat
    15781 tcgtttagtt tcgagtatga agaaaaaatt ttgtgaaaat ggctttccat atttttgcat
    15841 aaatttgact taataccgag aaactggact ttgaacaccg tttattggag aagtcatgaa
    15901 atcagcgcct tgacctgaat attagagaaa taaacagaaa aatatcaaaa aaccgaaaaa
    15961 ggacatcgcc ctttttcggt catttttagt gattatgaac gtgaatctgc tgtaacagaa
    16021 acatttgccc atgggtttgg tacatcataa gaccaatccc agtctttgac acggtcgttg
    16081 actggcaata cttcattacg gaacaacgtt ggaatcgcaa atgcttcttc aaatgcatat
    16141 tgttgccatt tttgatatgc ttctttttgg tactcttcat caaatgcttt gcttgaactg
    16201 atatcgttga tcaattttgt attttcttct gattcataac gagtgtagtt gaacgcagca
    16261 tttggtcccc atagtccact tggatttgga tcagttcctg tattccaagc agcttggtaa
    16321 acatcaattt caggatcatc gttttctaat ttatcataga aagaattgaa ttcgatcaga
    16381 cgacctgttg tgtattggac atccaaacca atttctttcc attgttggat atagtaatct
    16441 gccaatggtt gcgctgtttc cccaccggac atcgaagcaa agttgattgt tagtttattt
    16501 ccatctttgt cttctcttaa accatcgtca tccacatctt tgtagccagc atcatctaat
    16561 agttttttcg ctttgtctag atcttgtgtg aatcctttca tctcatcatc atggtatgac
    16621 tggaatactg gaggaatcaa tgaagtcgca ttgcttctca aacctgcata gaaacgttca
    16681 cctactgcat tattatctaa tgcatagccc attgcttgac gtaatgattt atcagccatt
    16741 tttgaatctg gattgtattc cacactcttt gtatctgcat tccatttacc taacttgaat
    16801 cctagataag tataagaagt ttgttcacgg cctaagtttg tatagccagc aatttcttta
    16861 taggtatcgt aagtatctgt tggcatttgg aaaatcatat catataattt agagttcaat
    16921 gcttcagttg cacttgccat cggtacagat ttcatcgtga tctttttcaa tttaggcgta
    16981 ccattccagt agtgctcgtt tggcaagtat tctactgatt ctcctgcaac gatattactc
    17041 atgtaataag ggccaaaagt cactggattt ttacgtactt gatcgcttga ttccatatct
    17101 ttgattgcta caccttctaa cgtatgtttt ggtgctgcat atgaccagat accaccaccc
    17161 gattgaagca tgcttgggtt tacttcttta tattcgattt caactgtttt gtcgtctatt
    17221 tttttgattc cggagattgt gtcggcactt ccatcatgat attcttccat acctacgatg
    17281 ttacggaaag tgtcgtcata acggatacca gtgtaatctt tattcccaat gatttcgtaa
    17341 gggaaaatca catcgtccgc cgttacatct tcaccatctg accatttgac gttgtctttt
    17401 actttgattg tcgctttttt cgtatcttca tctagatcaa gcgttgcagc tccgtcgtct
    17461 gtgattctga aatcgtcatc gtttgcaaat agcggttcat gagaaggagc cataaaagct
    17521 gcatcatatg aatcttggta aaactcccac tggaacaatc ctttaaactg actgtccgtt
    17581 gcgaccgcag cttctaattc gccgtcttcg attgcatcat cattattttt tgttgcaatt
    17641 ggaaaagtta attcctcttt gttattgccg ttgcctccag agttgtttcc agaggtatct
    17701 gtacctccgc ccccgcatgc tgcaagaacg acagcagaca tcagcgctgc aaatccaaaa
    17761 atcgttttct tcattcctat ttcccccttc gcttctatac tagcctaacc gttggcgcgc
    17821 atctgcagaa cgcttgaatg cttgtccaac ataattgata ctcaacatca aaactaaaat
    17881 aaggattgat gcgggtaacc agatccattg ccgattcact aagacttctc cgttacttgc
    17941 ataaccgatc aatgtaccta aactaggtac atttgccggc aagccgaaac ctaagaaagt
    18001 caaagttgtc tcgataccga tgtttgccgc aaagttcatc gtcaaattag taatgattaa
    18061 tgaacttaga ttcggcatga tttcgcggaa catgatttta aaatcattgg ttcccattgt
    18121 ttttgaggca ctgacataat ctcttcttgc ttcagataat gtcttgcttc ggaataatcg
    18181 ggctttagct acccaataaa atgcactcat gatgaatata aaggaccaga cattgtatct
    18241 ggaaataatc gatacgaaga cgataatgat cagcatgatc ggtagaatca tgataaaatc
    18301 aacgatccgc atcaagatgt tgtcaatgat ccctccgtag tagccagaga tgatccctaa
    18361 tccaaccccg atgatcgaag taaggatcgt gatcgcaaat ccgatcgtaa cagagtttct
    18421 agcacctatg atcaattgtc ctaatacatc acgtccgcct tcatctgctc ccagaatgaa
    18481 cttttgtcct tgtgtcgaaa ctgttcctgg tgcagcgtat ttgtcgaaga tactaacata
    18541 catcactttg tcttgatcgg tcagcatcgc accgataaag atcactaata ataaaaccac
    18601 tagcaaacct aatgaaaaaa tagcaatttt atctttttta aattctcgag caatcattcg
    18661 tatccccata ggaggaatac tttcttgaac tgctgtattg tttttttcac tcatcgatcc
    18721 attctccttt ctgattggtt ttattgaatc cgaatacgcg ggtcaacgat actcatgatg
    18781 atatctgaaa gtaatgtccc cactagcgtc gcgattccta aaatcaatac taaggatgtg
    18841 atcacggtat agtcccgttg gccgatggaa tcaataaata gttttccgac tcccggataa
    18901 ccaaaaatct tttcgataac tacagaacca gcaatcaacg aagtgatttc ataccccatt
    18961 tgagaagcca tcggcaacgc tgcatttcgg aaaatatgtc gactgaatac tttattcgtc
    19021 ggtacccctt tggaacgggc tgttctgaca aaatcgagcg atttcgaatc gattacttca
    19081 cttcgcagat attggattgt gacggcagtc gccaaaaatg cttgggtaat tgatggtaac
    19141 agaatatatt tcaaacggtc tatccattga gtgataaatc caccttgcaa acctagtgtt
    19201 tgtgatccac ttgttgggaa ccagcctaaa cgataaccaa aaacaaatag catgatcaag
    19261 ccgaagataa ataaaggaac agcaaaacta aagaagttgt agattacgac aatcttatcc
    19321 aagattgaat tttgataccg gccagcaagc atccccagag gcacagcaat caaataagta
    19381 aggacaactg tcaagatcga tagatataaa gtgttgatga tccgttcgcc gatcaagtca
    19441 gcaacagagc gtttgtataa gaaactttga ccaaaatcgc cttgcaaagc atttccgatc
    19501 caacggaagt actggacata ccatgggtca ttcaagccag ctgcttgtcg catctgttcg
    19561 atcactgccg gatcctgatt tggattgatc aaaccggtaa atggatcccc cggcatcgct
    19621 tgggctaata agaagatcag aacacttaaa ataataactt gcgggatcat caataaaaca
    19681 cgtcgcagaa tcgttttcca cattagcttg cacctccatc tttcaacgcc actttgtgtg
    19741 tagttgtgat cgtacgcaaa tcgtagactc gaccagtcgc atcatagtat tctttttggt
    19801 tttggatata ctcttgttct actcttcgtc gattttcttt gtggctttcc ctattttcta
    19861 catcgatttg cggaatagct gaaagcagac gtttcgtata gatatgtcgc gggtcattat
    19921 aaatgtcttc tcttgtaccg atttctacaa atcgaccttt gtacataatt gcgatattgt
    19981 cgcacatgtg ttttactaca cctaaatcat gtgaaatgaa taaatagctg aggccgtatt
    20041 cttgctggat tcgtttcatg aaattcaata cttgcgcttg aacagataga tccagagcag
    20101 atacaggttc atcggctacg atcagttttg gattggtagc aacagcacgg gctactccta
    20161 gacgttgacg ttgtccgcct gaaaattcat gaggatattt atatagcgca tcttccggca
    20221 tcccaacgat atctagcagt ccttttacct ttttcttttc ttcttgatcg cttaaccgtt
    20281 caaaatttcg gatgggttca gcaatgatat ccaacactcg ctttttagga ttcaaactgg
    20341 acattgaatc ttggaagatc atctgcacat ccttgttata acccattttt ttgcggttgc
    20401 ttcgcttcgt tacatctttt ccttcataca tgatttgtcc ggctgttacc ttttctaatc
    20461 ccacaacggc ttttcccgta gtagattttc cagagccaga ctctcccact aaaccatatg
    20521 tctttccttt ttcgataata aaatccacac catctacagc atatacatga tccgttactc
    20581 gattgaagaa tccactgcga atagggtaat gaactttcag gtctttgatt gtgattaatt
    20641 ctgtcatcta cacctctcct ttttcgtctc taaagtggaa atgcttatag caggagcaac
    20701 gtaccaagtg gttcggtgcg acttcatgaa ggataggctc ttcttcatga tcgcttgctg
    20761 gtatccatgg aatacgtggt gcaaaccgac aacctttacg aggcatgttt ttcagagaag
    20821 gaacgacacc ttcgatcaca tggagttctg catcatgatt tccttcttgt ggtatagagt
    20881 tcagtaaaga gcgtgtataa ggatgcttag gatgatcaaa cagttctttt acacctgcta
    20941 cttcaacgaa ctgtccgcca tacatcactg ccactcgatc agccatttct gctacgactc
    21001 ctaagtcatg agtgatcaag atgatccctg tttttgtttc ttcctgaagg tctttcagta
    21061 aatcaagaat ctgtgcttgg atggttacat ctagggctgt cgtcggttca tctgcaatga
    21121 cgattggagg tttgcaggcg attgcgattg cgatgatgac acgttgtctc ataccgcctg
    21181 atagttcatg gggatattgg cgtccaactc tcgcaggatt aggaatccct acttgttcta
    21241 gcaattctag tgcacgagct tgacgctgtt ctgcattcat ctttgtgtga taggaaagtc
    21301 cttctttgat ctggtcttcg attctcatga gaggatttaa tgcagacaac ggatcttgga
    21361 aaatcattcc gatatcattt ccgcggattt tattatagag cgtttcgttc aggtcgatta
    21421 aattcagatc gttatataaa atctcacctg taatttttgt gttattagga tcaagtaatc
    21481 ccataatcgt agtagccaag gtacttttcc cgcacccaga ttccccaacg attgctaaaa
    21541 tctcgttttt gtctaaagta aatgaaacgt catctactgc atcgtaatag tcatctttga
    21601 gtcggaatcc tgtatgtagg ttctgaactt ctaataattg gttatcggac aatcgaatac
    21661 ccctctctgc ttcctaaatg gaagtttttc ttaataaaaa aggacattac acagaataaa
    21721 tcgttttata acaaag
//
LOCUS       DASJUF010000047        21101 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJUF010000047 DASJUF010000000
VERSION     DASJUF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.047"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(449..1078)
                     /gene="udk"
                     /locus_tag="U4B62_001537"
     CDS             complement(449..1078)
                     /gene="udk"
                     /locus_tag="U4B62_001537"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN2141701.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1162..1857)
                     /locus_tag="U4B62_001538"
     CDS             complement(1162..1857)
                     /locus_tag="U4B62_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN2141702.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1870..2586)
                     /locus_tag="U4B62_001539"
     CDS             complement(1870..2586)
                     /locus_tag="U4B62_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN2141703.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2916..3524
                     /locus_tag="U4B62_001540"
     CDS             2916..3524
                     /locus_tag="U4B62_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN2141704.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3849..4172
                     /locus_tag="U4B62_001541"
     CDS             3849..4172
                     /locus_tag="U4B62_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN2141705.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4354..5724
                     /locus_tag="U4B62_001542"
     CDS             4354..5724
                     /locus_tag="U4B62_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN2141706.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5854..7155)
                     /locus_tag="U4B62_001543"
     CDS             complement(5854..7155)
                     /locus_tag="U4B62_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2141707.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7509..9467)
                     /locus_tag="U4B62_001544"
     CDS             complement(7509..9467)
                     /locus_tag="U4B62_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN2141708.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9644..9910)
                     /locus_tag="U4B62_001545"
     CDS             complement(9644..9910)
                     /locus_tag="U4B62_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2141709.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10420..12546)
                     /gene="pnp"
                     /locus_tag="U4B62_001546"
     CDS             complement(10420..12546)
                     /gene="pnp"
                     /locus_tag="U4B62_001546"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN2141710.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12734..13003)
                     /gene="rpsO"
                     /locus_tag="U4B62_001547"
     CDS             complement(12734..13003)
                     /gene="rpsO"
                     /locus_tag="U4B62_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN2141711.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13254..13817
                     /locus_tag="U4B62_001548"
     CDS             13254..13817
                     /locus_tag="U4B62_001548"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2141712.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14070..14591)
                     /locus_tag="U4B62_001549"
     CDS             complement(14070..14591)
                     /locus_tag="U4B62_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN2141713.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14594..15436)
                     /locus_tag="U4B62_001550"
     CDS             complement(14594..15436)
                     /locus_tag="U4B62_001550"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN2141714.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15429..16154)
                     /locus_tag="U4B62_001551"
     CDS             complement(15429..16154)
                     /locus_tag="U4B62_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN2141715.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16330..16626
                     /locus_tag="U4B62_001552"
     CDS             16330..16626
                     /locus_tag="U4B62_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141716.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17194..18786
                     /locus_tag="U4B62_001553"
     CDS             17194..18786
                     /locus_tag="U4B62_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2141717.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18799..19539
                     /locus_tag="U4B62_001554"
     CDS             18799..19539
                     /locus_tag="U4B62_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2141718.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19707..21056
                     /locus_tag="U4B62_001555"
     CDS             19707..21056
                     /locus_tag="U4B62_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2141719.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
BASE COUNT         6021 a         4291 c         4023 g         6766 t
ORIGIN      
        1 caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga aataattttc
       61 gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc gttaaacggt
      121 gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg aggagctgtt
      181 ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct ctacttcacg
      241 gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata aacagctatt
      301 ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct cttgttataa
      361 ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt tcaaacggtc
      421 gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta gctacttttg
      481 ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg atatccgcat
      541 agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc aaatattgtt
      601 cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata cgacgaatga
      661 tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca cgcagacgtt
      721 cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc tgaatgatcg
      781 ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg gctttatagt
      841 tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat gggtgatcgt
      901 aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa taagaatctt
      961 gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg ctgacacttg
     1021 tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt tttgtcattg
     1081 actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa aaaacacgaa
     1141 aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat aaagttcctc
     1201 taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt tatttctatt
     1261 gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag tcaaagaatc
     1321 accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga catgatcaaa
     1381 aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat ccttgaagta
     1441 atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac cgttcgtcac
     1501 aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc ctggaacaag
     1561 atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac tatctactgt
     1621 tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt ctcgtgatag
     1681 tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc tggtttcgat
     1741 ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat tggcttcagt
     1801 taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat atttcatctt
     1861 tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg tcaacgatcg
     1921 atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta gccgtttcaa
     1981 tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga attttccttc
     2041 ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt ttaaatcagc
     2101 cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg ttacgtctgc
     2161 cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt tttctggaag
     2221 tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg ttgtatctct
     2281 atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa tcagatcgat
     2341 tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt tattgagaag
     2401 ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat gcagtaaact
     2461 atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt gcttcaaagc
     2521 aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta atttatctaa
     2581 acgcatgctg tttcctttct tattactact aattaattat accttgaata ttcttgcttg
     2641 caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac ggttaaatag
     2701 taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa gttgaatcct
     2761 gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc gccctgcctt
     2821 cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt ccgttaccaa
     2881 agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta gaagtaagta
     2941 aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg aaagttccag
     3001 ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt ttgacactaa
     3061 gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc gatggacaaa
     3121 aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat ttcgaacatg
     3181 cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa gtcgttcttg
     3241 ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg tatacagtag
     3301 tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg acaaacttgg
     3361 aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac aaattcgtga
     3421 ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa gaaagcacag
     3481 gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga aacaatcagg
     3541 tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc taatcgttta
     3601 ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc ctaatttacc
     3661 aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt gaaaaaaagt
     3721 attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg ttgaagtaag
     3781 tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat tcgaaggagg
     3841 ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca gttggtgtcg
     3901 tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac ctagatctta
     3961 agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat cgtggaaaag
     4021 tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat ttgccgtttg
     4081 cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg agagaaacga
     4141 tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg atgtccctta
     4201 ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc tttttatgga
     4261 gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct atatactact
     4321 aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa aggaagcccc
     4381 gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt tcagcagttc
     4441 tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc gctggcagcg
     4501 gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg tttaacagct
     4561 ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg tctgaagaaa
     4621 agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac tgtcctaagt
     4681 ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat acttgatcaa
     4741 gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag tttcaatttg
     4801 ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt tctgatcatt
     4861 gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg catgggtgtg
     4921 gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact gtgtctagtg
     4981 tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa atttgaaaaa
     5041 gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc ttcgatcatc
     5101 gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga tgtcgtagct
     5161 gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat gtcatttggt
     5221 attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg ccgaatcttg
     5281 caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc aggtgctatc
     5341 gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga aacacgtgta
     5401 tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat cttagcagtc
     5461 ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc tacgatcgcc
     5521 ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg aatgtgggga
     5581 tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc cgtgttgctt
     5641 tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga acatttcaca
     5701 ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc taaaaatgaa
     5761 aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga taacaaaaag
     5821 gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt ccgtggactc
     5881 tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac ggaatgatgc
     5941 aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa agcccgcaag
     6001 tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac gctggaaatc
     6061 atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg ctatcaatgc
     6121 ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa tatcttcaat
     6181 cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat gcttaccctt
     6241 ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta cgatcggctc
     6301 ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga tcaaatctgg
     6361 caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat agcgagtatg
     6421 tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt cgaatacttg
     6481 catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt caaacacgtt
     6541 ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat tcaatgcgac
     6601 taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac gcacacctaa
     6661 tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa acattacttt
     6721 ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat tgacgatact
     6781 atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta aaataaatcc
     6841 cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac cgatcactgg
     6901 ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac cggttagcgt
     6961 aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat gaagagctag
     7021 cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc tgattttcac
     7081 taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa ataaaataat
     7141 gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt tttctacaaa
     7201 aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg gcatttttca
     7261 gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca gctttttcat
     7321 gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc caaacagatg
     7381 aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag tacagctttt
     7441 tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt caggtcaaag
     7501 cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat gataagcgga
     7561 acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg cgaggtagcg
     7621 atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc tagagttgtc
     7681 acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc tcgccctggt
     7741 gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa atcatggatg
     7801 acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa cagattcact
     7861 tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata tggctcatcc
     7921 gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt atctaaaatc
     7981 gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa gaagaccaca
     8041 tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg cgggatataa
     8101 tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg ttcccaaata
     8161 aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc tcggaagaat
     8221 ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa ggtaactaaa
     8281 tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag tgctgttgtc
     8341 atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg agacgttcgg
     8401 aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc tgggatatac
     8461 atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag tttcaacttg
     8521 attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc aatgactgca
     8581 gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat ttggaagaat
     8641 ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa caaccacgca
     8701 gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct cagacgaata
     8761 tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt cgtagccagg
     8821 aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag cagctggata
     8881 gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt cattaatccc
     8941 attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt tccgacaata
     9001 tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt gataatgacg
     9061 atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg caagccttca
     9121 atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa ggcagctcct
     9181 ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt gtagagtgag
     9241 aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata tttgattgtt
     9301 gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa atcaggattg
     9361 attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag cggacttgtc
     9421 ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga tatctttttg
     9481 gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata tcctttaggt
     9541 aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa ttgcttctaa
     9601 ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct ttctttggac
     9661 gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt gcacccaata
     9721 atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt aaaaattcgc
     9781 cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc gggacttcct
     9841 ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac gaccataaaa
     9901 aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt acgctttatt
     9961 cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt gtaacaaaag
    10021 tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag gttgtgaaat
    10081 cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca tattttgccg
    10141 ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag aggttgtaaa
    10201 aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct aatgttttta
    10261 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa
    10321 ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg tccccctgtc
    10381 accttcacga tcacaagtac attattccta tgaattaaat tatttttctt catttttcgt
    10441 tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt gtttatcgat
    10501 ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga ctttattgac
    10561 acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga tcaagttgac
    10621 gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac caactttgac
    10681 ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg catcagctga
    10741 agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt catcgatgat
    10801 tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag gttcgatctg
    10861 gatcatttcg atttttggtg catattggct taattcttca cgtggagcag caatcgttga
    10921 agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg cttcagttag
    10981 gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga taccatcttt
    11041 tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt ggatatcagt
    11101 taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg cgactggcgc
    11161 tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac ttgcttggga
    11221 agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag gaaattcttc
    11281 ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc cgatttcccg
    11341 acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga aattgtagtg
    11401 gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt gctctcctag
    11461 aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca aacctgatcc
    11521 atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat ccaatttacg
    11581 cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt tttcaagatc
    11641 ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac ctgcaaattt
    11701 ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt cgcgagcaag
    11761 tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa agatttcttt
    11821 tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc ctactgcttg
    11881 aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa acagcaatgc
    11941 accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat tgatggcttg
    12001 tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta ctgttggatt
    12061 caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc cgtcaaatgg
    12121 gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag ctggcgagca
    12181 gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga agccttctgc
    12241 aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt ccgtacttgg
    12301 acggccttct cgtttgatga aacctccagg gattttccct gctgcataca ttttttcttc
    12361 ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg ccacagctgc
    12421 acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt gttttgctag
    12481 ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata cttgtttttc
    12541 tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca aatgaaaact
    12601 gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc tagcttgatc
    12661 acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg aatcccactc
    12721 tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg taacgttgaa
    12781 cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt ttcattaggc
    12841 cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag tggttgattt
    12901 cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca tggcgagcgt
    12961 attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc acctctttcg
    13021 attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg gcaggtcatg
    13081 tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag tagttaatca
    13141 ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg tggtaaacta
    13201 taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat tccatgatta
    13261 caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca aaagaagttt
    13321 ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag ttcttaaaaa
    13381 acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt ggcttagctg
    13441 ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt cctgacccag
    13501 aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg agccattctg
    13561 ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga gaagtaccag
    13621 gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat ggtgaaaaac
    13681 acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc gaccatatca
    13741 acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta aaagaaggcg
    13801 tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca agaaagcttt
    13861 ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc cactaatcga
    13921 aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg gatacgcatc
    13981 ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg atagaatagt
    14041 ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc tgcttcagct
    14101 agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt atgcagatgg
    14161 atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg tctcataaag
    14221 gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc gtaaagggga
    14281 tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct cagttcttct
    14341 tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt attggtttct
    14401 tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc agctcgtagt
    14461 aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc aaacttgcta
    14521 gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg ccgtgtctct
    14581 ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag tgtttcatca
    14641 aatcaacata cgtatctgtt attgtttcct tatatccctt atttttcagc agactaacaa
    14701 ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt tcttcttttt
    14761 tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca atcaactctc
    14821 gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt agttcttcct
    14881 ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg ggaaaagatt
    14941 tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca cccggcgtga
    15001 cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt gcgtactcca
    15061 gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc tggacattcg
    15121 gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat gtacaattga
    15181 aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa ttgataactg
    15241 gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat tgggatcgca
    15301 gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac atacggtcgg
    15361 caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt tctttttcgg
    15421 ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac aaccgctccg
    15481 tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt gattactaag
    15541 attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac ttgaaaactc
    15601 gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg ctccatgact
    15661 ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc tccgcatgct
    15721 tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca ttttcgacaa
    15781 tgacattgat acaaacttcc atgaaaattg acgagttgcg ggcttttagc ctttgcgtgc
    15841 aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag atgtgccatt
    15901 tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac aaattgatag
    15961 aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg ctgctccatc
    16021 ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt ggatacccct
    16081 gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc ttcgtctact
    16141 gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc gaacaaattt
    16201 attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg aacaatttat
    16261 cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat aaaagtgagg
    16321 tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg tatcgcctgg
    16381 ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa tggaaatctt
    16441 tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac attagccgta
    16501 tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct aatattagcc
    16561 atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa gaaagcggca
    16621 gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt ctttttttat
    16681 tgtattacat acatttttgt gtataattat actatcgttt gcaatggata gatgattcac
    16741 attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat tttttcaaaa
    16801 aacagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt gtataaataa
    16861 ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta accctgtgga
    16921 gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca cttttttgct
    16981 tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag tgatgctaag
    17041 ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc tttttatatc
    17101 tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca tctagaccgc
    17161 tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt ttcagttgcc
    17221 gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa tgcagatgaa
    17281 aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc cttcaactgg
    17341 tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag ttacgccggc
    17401 ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa attagtcatc
    17461 gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat cgacgcgatc
    17521 atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac cgatccttat
    17581 tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa agcaaaaagt
    17641 ttagcagatc tttctggagc aaagattact gctcagttaa atacattcca ctatactgtg
    17701 atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc agcaatgaga
    17761 acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga aggtgtaacg
    17821 gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga tggcttccag
    17881 acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc ggatatccaa
    17941 aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat tatggaccaa
    18001 gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat catttctgat
    18061 ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg acttaccttg
    18121 ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat tggtgtcttc
    18181 cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat cggaaatgca
    18241 cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca agcaatggtc
    18301 atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat tgccgctttg
    18361 tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg ggggattttt
    18421 gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca tggccaaacg
    18481 atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac aggaaatgag
    18541 tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc tgatttgttc
    18601 ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt tacgatcgtc
    18661 gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc ggtagaaaag
    18721 aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc aaatttgcag
    18781 gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct aagaaaaagc
    18841 ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg tgaagttgtg
    18901 acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat caatttactg
    18961 gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc tcctaaatat
    19021 aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa cctgttcaac
    19081 aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa acgagataaa
    19141 gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga tcgttatatc
    19201 gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat tgctcgggca
    19261 ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt agaccctgaa
    19321 atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt gacaatgctt
    19381 atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat ttttatggat
    19441 aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc gaaagaagaa
    19501 cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct gattgggaca
    19561 aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag gtaaatttct
    19621 gaaaattttg gaatcttagt tgtgtttttc agaaaactct tttaatatgg aagtaattaa
    19681 acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag cgtggactga
    19741 aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg ttgtttttag
    19801 gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat cttattactg
    19861 gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca aacaccaata
    19921 accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct ttcatcactg
    19981 gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca gccatcggaa
    20041 tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg attgcattag
    20101 gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta gagttttggt
    20161 ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct tatatgatcc
    20221 tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg tctcatggcg
    20281 gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta acctttgctt
    20341 ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct gaaaaagtgt
    20401 tgccggaagc tatcaataat attcctatca ggatcatctt attttactta ggatctcttt
    20461 ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt ccatttgtag
    20521 aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta gtcgtattaa
    20581 gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg cttcgttctt
    20641 tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat catgtgccag
    20701 ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg aattacgtga
    20761 tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc ttgtttatct
    20821 ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc aaaaaagctc
    20881 attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt tacatggcct
    20941 ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta ctaacacctg
    21001 tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag aagtaagaaa
    21061 gtagttgtga cagaagtggt cagcttcgag aaataagaag g
//
LOCUS       DASJUF010000048        20360 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJUF010000048 DASJUF010000000
VERSION     DASJUF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.048"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            291..1430
                     /locus_tag="U4B62_001556"
     CDS             291..1430
                     /locus_tag="U4B62_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN2141720.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="U4B62_001557"
     CDS             complement(1497..2072)
                     /locus_tag="U4B62_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2141721.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="U4B62_001558"
     CDS             complement(2077..3051)
                     /locus_tag="U4B62_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEN2141722.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="U4B62_001559"
     CDS             3334..4302
                     /locus_tag="U4B62_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2141723.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="U4B62_001560"
     CDS             4415..4693
                     /locus_tag="U4B62_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2141724.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="U4B62_001561"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="U4B62_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN2141725.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="U4B62_001562"
     CDS             5150..5905
                     /locus_tag="U4B62_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2141726.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="U4B62_001563"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="U4B62_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN2141727.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="U4B62_001564"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="U4B62_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN2141728.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="U4B62_001565"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="U4B62_001565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN2141729.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="U4B62_001566"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="U4B62_001566"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN2141730.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="U4B62_001567"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="U4B62_001567"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN2141731.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="U4B62_001568"
     CDS             10642..11184
                     /locus_tag="U4B62_001568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN2141732.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /locus_tag="U4B62_001569"
     CDS             11207..12763
                     /locus_tag="U4B62_001569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN2141733.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="U4B62_001570"
     CDS             12777..13679
                     /locus_tag="U4B62_001570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN2141734.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="U4B62_001571"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="U4B62_001571"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN2141735.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="U4B62_001572"
     CDS             15122..15544
                     /locus_tag="U4B62_001572"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN2141736.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="U4B62_001573"
     CDS             16013..16243
                     /locus_tag="U4B62_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN2141737.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="U4B62_001574"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="U4B62_001574"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2141738.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="U4B62_001575"
     CDS             17654..17830
                     /locus_tag="U4B62_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2141739.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="U4B62_001576"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="U4B62_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN2141740.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="U4B62_001577"
     CDS             18325..18708
                     /locus_tag="U4B62_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2141741.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="U4B62_001578"
     CDS             18877..19761
                     /locus_tag="U4B62_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2141742.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="U4B62_001579"
     CDS             19875..20111
                     /locus_tag="U4B62_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN2141743.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="U4B62_001580"
     tRNA            20199..20279
                     /locus_tag="U4B62_001580"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="U4B62_001581"
     tRNA            20286..20357
                     /locus_tag="U4B62_001581"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DASJUF010000049        19480 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJUF010000049 DASJUF010000000
VERSION     DASJUF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.049"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="U4B62_001582"
     CDS             133..3660
                     /locus_tag="U4B62_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN2141744.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="U4B62_001583"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="U4B62_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN2141745.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="U4B62_001584"
     CDS             7448..7789
                     /locus_tag="U4B62_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2141746.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="U4B62_001585"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="U4B62_001585"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN2141747.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="U4B62_001586"
     CDS             9146..11566
                     /locus_tag="U4B62_001586"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN2141748.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4B62_001587"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4B62_001587"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN2141749.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="U4B62_001588"
     CDS             13000..13737
                     /locus_tag="U4B62_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2141750.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="U4B62_001589"
     CDS             13751..14566
                     /locus_tag="U4B62_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2141751.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="U4B62_001590"
     CDS             14579..15226
                     /locus_tag="U4B62_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2141752.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="U4B62_001591"
     CDS             15242..15898
                     /locus_tag="U4B62_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2141753.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="U4B62_001592"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="U4B62_001592"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN2141754.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="U4B62_001593"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="U4B62_001593"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN2141755.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="U4B62_001594"
     CDS             18240..18758
                     /locus_tag="U4B62_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2141756.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="U4B62_001595"
     CDS             18865..19365
                     /locus_tag="U4B62_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2141757.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3344 c         4182 g         5402 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DASJUF010000050        19355 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJUF010000050 DASJUF010000000
VERSION     DASJUF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.050"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(320..1345)
                     /locus_tag="U4B62_001596"
     CDS             complement(320..1345)
                     /locus_tag="U4B62_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2141758.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1620..3305
                     /locus_tag="U4B62_001597"
     CDS             1620..3305
                     /locus_tag="U4B62_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN2141759.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3319..4602
                     /locus_tag="U4B62_001598"
     CDS             3319..4602
                     /locus_tag="U4B62_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2141760.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4672..5541
                     /locus_tag="U4B62_001599"
     CDS             4672..5541
                     /locus_tag="U4B62_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2141761.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5538..6374
                     /locus_tag="U4B62_001600"
     CDS             5538..6374
                     /locus_tag="U4B62_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2141762.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6400..7467
                     /locus_tag="U4B62_001601"
     CDS             6400..7467
                     /locus_tag="U4B62_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN2141763.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7464..8291
                     /locus_tag="U4B62_001602"
     CDS             7464..8291
                     /locus_tag="U4B62_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2141764.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8336..9361
                     /locus_tag="U4B62_001603"
     CDS             8336..9361
                     /locus_tag="U4B62_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2141765.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9358..11541
                     /locus_tag="U4B62_001604"
     CDS             9358..11541
                     /locus_tag="U4B62_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2141766.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11541..12671
                     /locus_tag="U4B62_001605"
     CDS             11541..12671
                     /locus_tag="U4B62_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2141767.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12668..13345
                     /gene="pgmB"
                     /locus_tag="U4B62_001606"
     CDS             12668..13345
                     /gene="pgmB"
                     /locus_tag="U4B62_001606"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2141768.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13429..13956
                     /gene="msrA"
                     /locus_tag="U4B62_001607"
     CDS             13429..13956
                     /gene="msrA"
                     /locus_tag="U4B62_001607"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2141769.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14157..14843)
                     /locus_tag="U4B62_001608"
     CDS             complement(14157..14843)
                     /locus_tag="U4B62_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN2141770.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14923..16461
                     /locus_tag="U4B62_001609"
     CDS             14923..16461
                     /locus_tag="U4B62_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN2141771.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16581..17048
                     /locus_tag="U4B62_001610"
     CDS             16581..17048
                     /locus_tag="U4B62_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN2141772.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17567..18010
                     /gene="rplM"
                     /locus_tag="U4B62_001611"
     CDS             17567..18010
                     /gene="rplM"
                     /locus_tag="U4B62_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN2141773.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18026..18418
                     /gene="rpsI"
                     /locus_tag="U4B62_001612"
     CDS             18026..18418
                     /gene="rpsI"
                     /locus_tag="U4B62_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2141774.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18506..>19355)
                     /locus_tag="U4B62_001613"
     CDS             complement(18506..>19355)
                     /locus_tag="U4B62_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2141775.1"
                     /translation="NTEIISDELTKKSKEYANIKIIKGYVRSMFDIAEILNYIEFNRT
                     TKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMI
                     TLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTL
                     LSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDL
                     VHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQR
                     GLNKCD"
BASE COUNT         6250 a         3506 c         4113 g         5486 t
ORIGIN      
        1 aaccagtaga catgtctgtt attctattta tgttttagag ataagtatcc ccaaataaca
       61 tggattgaaa taagggtaaa tgtgtttaaa aaaacgaact ataaaaactt atacacaacg
      121 tggttaaaaa tagttttttc ttccctgtgg aaaaaatagt ttaacacaaa cattatagtt
      181 atccacagct atctaaaaca gatggaaaat aagaaaaatt cgctaaaata ccgggagggg
      241 gtaacgaggg atgagtgatt gaaaaaagaa cagagtaact gacttatcct cagtgtttct
      301 gttcttttat aattctgttc tacatttctt gttgttggat cgaatctgtg agagtaatct
      361 cggttgaaag aatgattctt ttttcttcag ggtattggtg gtggattagt tggaataaaa
      421 gttctgtggc tttgattcct aactgatttg tcggaatgtc tatccgagtc aactgtggcg
      481 aaaataggga ggacaataag ggatcactga atcctgcgat cgaaagatca tcaggtatct
      541 gctttccaga ttgttctgcc actttgtaaa tactcaatga ttttaatgca tcggtagaaa
      601 aaatcgcagt tggtctttct cgtagcttca ttaaatcatt cattgctgcc atgctatctt
      661 ctaagcgaaa gccgccgtct ataatccatt cattacggat atctaggtgg tgatcaaata
      721 agcattgacg gtaaccattg agacgatcat tggatacata aacttctgtc ggagcatgga
      781 gacagccgat atgacggtgt ccattcttga tcagtgtttc caccaaatcg taactatcct
      841 ggaaattatc cgtgtccact gagtagatgt tcgtgtagtc gtttccgact tttccgatta
      901 ccacaatagg aatattgtat tgatccaatt caaaaagaag tgtttcatcc atcgtagagc
      961 ttaaaataat aattcctcgg atcatttttt cttggatctt tttcgcgcat ttttctagct
     1021 cttcttctgg tgtttttgcc gtttgtagaa tcaagtcgaa gttattttgt tcagaaacgg
     1081 cagaaatgga ttgaataaca tctgaaaaaa aaggattgcc ataggtcgta tgagaagtac
     1141 gatgagataa tgctagaatc gcgtcaaaac catttcctga cagtcctctt gcaattttgc
     1201 tcggttcata attcaattct ttgattgctt gaagtacact tgcttttgct ttatcagaga
     1261 tatttggctg gttatttaat actctggaga cagttgattt tgagacatta gcttttttcg
     1321 caatgtcata aatagtaggt ttcaatttta tatgcacctc ctttttggga gcgttttaga
     1381 ataattccat agtacaatag acattctttt ttgtctatga ttttgaaaat ttaggaatag
     1441 cttgcatgat catgagagac catatttttt taagaagcta ttaattattg atacataagc
     1501 tttcttattt gtatttttgt gtatatcaaa attatttaaa aaaatgctat tgacagtaag
     1561 cgttttccat aatatgatca aatcgttggg aacgctccca aaaaagaaag gggaatccga
     1621 tgtatacaaa acttttacaa gcgatttatc gcgactcaga tttgtctgag aaaaccgcct
     1681 ctatgattga acaaaaagtc agtgtttatc agcagaagaa aggccaggca ggcaaactgc
     1741 attgggacga agaagatatt ttcttgatta cttatgggga tcaatttttt gaagagcagc
     1801 aaccaacttt gcgtacattt aagaaatttt atcaaaagta tttggcagat acatttagag
     1861 ttgtacattt cttgccattt ttcccatata catctgatga tggtttctcc gttgtaaatt
     1921 atcaggcaat caacgaccgt ttgggcacat gggaagatat agaagaaatg agaagcgaaa
     1981 cacggttaat gttcgacttt gtctgcaatc atatgtctgc cgaaagtact tggtttcagg
     2041 aatatttaaa cttgaatgat acgtacaaag atttttttat tgatttgcca ctggatacag
     2101 atacaagcag cgtgactaga ccaaggacca gcccgttact tacaacgttc attgacaaaa
     2161 acgggaaaga aaaacacgta tggacgactt ttagcgaaga tcaagtagac ttgaatttta
     2221 aaaatccaga agttttattg aaaatgatcg atattctttt gttttactta gaaaaaggcg
     2281 cttcatttat tcgcttggac gctgtgggat ttttatggaa agaactagga acttcttgta
     2341 tacacctgcc agaaacgcat aaaatcatcc aattattccg tgcaatcgct gatgaagcag
     2401 ctccaggaac ggtattgatc actgagacaa atgtgccaca caaagataat atcagttatt
     2461 ttgggaatgg cgagaatgaa gcacagatgg tctatcagtt ttcgctacca cctttgaccc
     2521 ttcaagcgat tcgttcgggt aatacaagta agttgaaaga atggttaaaa accatcgatt
     2581 tcgcatcgaa aaaaacaact ttttttaatt ttttagcctc ccatgacggt atcggattga
     2641 atccggtacg aggtattctg ccggaaaaaa cgatccttga attagtggaa cagctgaaga
     2701 aagagggtac ccgagtaaac tacaagaaaa acccagatgg aacgaccagt ccatatgaga
     2761 taaacgctac gtatctagat gcgttaagtt ctccaacaga tacgacggaa tggagaagca
     2821 aacgcttttt agctgctcat tcatttttgg taacgttacc aggcgtgccg gcaatttata
     2881 ttcaaagtgt gctgggaagt cgaaatgatt acaaaggcgt agaaaaaaca ggagagaatc
     2941 gaaaaatcaa tcggcaaaaa tttttgttag cagatattga gaaagagttg cagacagatc
     3001 cattaagaac agcagtatat ggtgaattga cccagatgct atctattcga aaaaaagaaa
     3061 aagccttcca tccgaatagt ttgatttact tactggactc agcagattct tttatcagtt
     3121 ttattcgcga agcgaaggaa gagaaagtct tagttatcca taatttttcc aatcaatcag
     3181 caggttatct tttaccaaaa ggagattacc tagacctgtt ttctggacaa attatccatt
     3241 gcagtcaaga acgaaagttg atattccagc cgtatgaata tatgtggctg aaaataataa
     3301 aataagaggt ggaaaaaaat gaaaaaaatt attgttacta cctgtctgct agctactgga
     3361 tttcttctta ctgcttgtgg tgaaagtcaa agctctaacg aatcacaaca gacaacgatt
     3421 caattcatgc actcttctgt cgaacaggaa cgattaagtg tgatcaatga actggttgct
     3481 gattttgaaa aagaaaatcc agacatcaaa atcaagcaag tgccggtaga agaatcagcg
     3541 ttcaatacaa aaatcgttac gttagctcgt tccggtaaat taccagaagt aatggaagtc
     3601 agtcaggatt ttgctaaagt gatggataaa gatgaactga ttgatcagac ggcagtccaa
     3661 aacgttatcg aggaagctgg agaagataat tattatgacg gagccaaaaa ccttgttcgt
     3721 tcagaagatg gaaaaagcta tattgctgca ccaattagcg gctgggttca aggtatttgg
     3781 tatgacaaag aaaaattatc agaagctggt ttttcagaac cagaaaattg ggatgatatt
     3841 ttaaaaattg ctcaacattt cacagataaa gagaataaac aatatggaat tgctatgcct
     3901 actgctgaag gaacaatgag tgagcaagcg ttctctcaat ttgctttgtc aaatggagca
     3961 aatgttttgg atgatgaagg cgaggtaacg atcgatacgg aaaagatgag agaagcattg
     4021 tcgttttatc aaaacttatc ccaatacacg atgccaggct ctaatgatgt gacagaaatc
     4081 aaagatgctt tcatgaatgg aacagcacca atggcaatct attcaactta cattttgcct
     4141 tctgtttatg aagcggggaa ctcagaaaat atcggttttg ctatcccaac acaaaaagat
     4201 caagctgtct atggcacagt atctggattg acgatttctt ctggtctaga cgagaaacaa
     4261 aaagaggctg ctgaagcatt cacagctttc ttatctcaac cgaaaaacat ggaaaagtgg
     4321 gtcttgatgt ctcctggtgg tgctcaacca gtgaataaac aagtagtcga actggatggt
     4381 tacaaagaaa atgaagtcat caagtctttt ggcgagctgc catctgagat tgctagtgct
     4441 tttgatgaag tacaagtctt tggactagtc ggggaaaaga atttcacgaa aatgggagat
     4501 atcacaagct caggtgtgat cggaaaagca gtgaaccaag taactgtcgg aaacgaagat
     4561 gtcgatcaag ctttagctga cgcacaaaaa aatacgaaat aacaagaagt tctgccaatg
     4621 gtgttcgaaa aaagtagata gctattggca gattatcagg gggaggtttc aatgaatcgt
     4681 ccaaggaaac attctgactt aaaattagcc atgggacttt tagcaccaag catgctttta
     4741 ttagctgttt tagtgctgat tccaatgctt tccaatattc agatcagttt tttcgatatg
     4801 ccgctgaatc ctaactcaga cgccgttttt atcggattaa gcaattatct tgaaatttta
     4861 agtgatagtg aattttatcg ttctttaggt ttaacagttg cttacacatt cttagttgta
     4921 gcaggcagta cagctttagg attagccgta gctattttgc ttaaccgcaa gttcagattt
     4981 aggaaaacgg tgcgttcact gattttatta agttatgtta cgccatctat ttctttgatt
     5041 tttgcttgga aatatatgtt caataatagt tatggaatcg tcaatgatct gttagtagat
     5101 aggttgcagc ttttcaacga agcaccgctg tggttcgacc agccagttgc tagcttcttt
     5161 atggtcgttt tgtttgctat ctggagatac tatccgtatg cgtttatttc atttttggct
     5221 attttgcaaa cagtcgacaa aacgctttat gaagcagctg aaatggacgg tgcaaatgct
     5281 tggcagaagt tcaagatcgt gacactgccg gcaatcaaac cagtactagc aactgtgatt
     5341 gttttacgat ctatctggat gttctatatg tttacagatg tctatctact gacgaataaa
     5401 gtcaatatct taggcgttta tttgtatcag acagcctttg cgttcaatga tcttggaaaa
     5461 gcagcagcta tatcgattct attgttcatc attattgcag cggtcgtctt gctctcacga
     5521 aagaaggtgg acttgaaatg aaaaataaaa agagcaggaa aatcttattt tacgcgacga
     5581 ttttgttgct attagctgtc aatttgtttc catttttgat catgctgatg acatcattca
     5641 aggattcaca agaagcaatt gcaacgacac aaactatttt tccgaaagaa tggacttttg
     5701 aacattatca ggatattttc aatgctgaga tttttccatt catcatgtat ttcaaaaata
     5761 gtttagtgat ctccttgata gcctccgtaa ctgcagtagt gattgggatc agcggggcat
     5821 acgctttgtc aaaactacgc ttcaaagctc gctcgaaaat caatgctagc ttttacaccg
     5881 tttatatgtt ttccggtatt ttgatcgttg ttccattatt taaaatcatt tccggtctag
     5941 gtttgtatga taccaataca gcactgatta tcacgatggt cgttcaaaca cttcctactg
     6001 cgatattcat gctaaaaagt tattttgaca cgattccgac tgatattgaa gaagcagcaa
     6061 tgatcgatgg tcttagccgg atccagatca ttattcggat cattatacca ttggctattt
     6121 ctggaatcat ttctgtcttc gtttattgct ttatggtttc gtggaatgat tatttatttg
     6181 cttcgatttt cctgtcgacc tccgaaaaat ttactttacc aatcggtttg aacacattat
     6241 tcagtacacc agactacatt tggggacgaa tgatggccgc ttcattggtg accgctttac
     6301 cagtcatcat tatgtatgcg ttatctgaac gatttattaa aggtgattta gtcgaaggcg
     6361 gcgtaaaagg ataaaaagaa aagaaagagg aaagaaaaga tgaaaaaatt agttgcatta
     6421 gaaccaagaa tagccggatt caaagattac gaagaccgtg agatcgaaaa gaacgaagta
     6481 cgtatccgag taattttcgc tactccaaaa ccaggaacag aaattgtcga ctttagagga
     6541 ttaagtccat ttatcgatga agaattttca gaagaatggc gcctattctt gccaagagaa
     6601 gaaggaagcc aacgcggtgt ggtatttggc gaatttcaat taggaaatat gattgtcggt
     6661 acgatcgaag aaacgggcag tttggtgaca gagtatcaaa aaggagagtt agtctgcact
     6721 tatggaccca ttcgtgaaac gctgattaca aacggtgttg ataattataa actacgtaaa
     6781 cttgatcggt tggatcaatg gaaaaatgca gtatgttacg atcctgctca attcgcttta
     6841 ggtggtatcc gggaagcaca tgttcgagca ggtgatgctg tagcaatctt cggtttaggc
     6901 gctatcgggc aaattgcgat tcaattagct aaaaaagcag gtgctactac catcgttggg
     6961 atcgatccca tagcgcagcg tagagaatta gcagaaaaat acggtgccga ttacacgcta
     7021 gatcctatga aagcagatgt cggttttcgt ctaaaggaat tgacagataa acgtggagta
     7081 gattcagtta ttgaaacgag tggcagtgct gccgcattac aagcttcttt aagagggatc
     7141 ggctatggcg ggatcatttc ttatgttgcc tttgctaaac cttttccagc tgggtttaat
     7201 cttggtcgcg aagcacattt caataatgca aaaatcgtct ttgctcgagc atctagtgaa
     7261 ccaaacccag attacccaag atggaaccgt aaacgaatcg aggacacatg ttgggaatta
     7321 ttgatgaacg gttacttgga ttgtgaagaa atcattgatc cagttgtgcc atttgcagaa
     7381 agcgcagaag cttacatgcg ttatgtagac cagcacccag aagaaagtat caaaatgggc
     7441 gttcaagtcc gaggaggaga acaatgaaat tcggtacgca agatcaagca tttttcccaa
     7501 gagacatttt agaaaaattt agatttatca aagactgtgg atttgaagaa tacgaaatcg
     7561 acggtaagtt gttaatagaa aatatcgaca cagtcaaaca ggcaatcaaa gaaacaggga
     7621 tcccagtagt gacagcttgt aacggctacg atgggtggat cggtgacttt atcgaagaac
     7681 ggcgtcttaa tggtgtacgt caaattgctg agattttgcg tgcactaagc gaggttggag
     7741 gcaaaggaat cgttatccca gcagcttggg gaatgttcac ttatcgatta ccgccgatgg
     7801 tttcaccccg aagcaaagaa ggagactata aagcagtcag cgaatcactt cgttatttag
     7861 ataaagtagc cgaagaaaca ggtacggtta tttatttgga gcctttgaac cgataccaag
     7921 accatatgat caatttgtta gcggatgcgc gtcattacat tgactctaat caattgaaac
     7981 atgtgcagat catcgctgat ttttatcata tgaatattga agaagatgat atttcaaaag
     8041 cattgactac gcaaaaagag tatgtgggtc atatccattt agcagataat catcgctacc
     8101 aaccaggatc tggcgcaatt gattttactc gccattttca aacgctactt caaaatgagt
     8161 atgaaggtta tatggtatat gaatgtcgtt tgcgaggaga aaatgaagtg aaaatttatc
     8221 aggaatcatt gcaatacttg aaaaactgtc tggaaacagc gcgtcattta aatgatttag
     8281 agaaagaata gagaaagaat aacaataagt aaataagcaa aggagggaga aataaatgaa
     8341 gaaagtagca atcataggcg ctggacaagt agcagaaaaa gtacatgctg cttattatca
     8401 aaaaagatca gatgtcttag aattggccgc tgtggttgat ccagtcagcc agcgagcaga
     8461 agacttttgc cgacgtacag gattttctca tgcgtataca tcagttacac aaatgctgaa
     8521 tgagatttct cccgatatcg tgagtgtctg tacacccaat cgctttcact tcgaacatgt
     8581 attggctgcc ctcgaagcta atgcttcagt gatgtgcgag aaaccaccag ctatgacagc
     8641 tgatcaagca agaaagatgg cagagacagc cgataaaaac gggcagattt tggcttatgg
     8701 attccagcat cgtttttctg acgaagcaac aatgatcaag aatcatcttg atgttctcgg
     8761 ggatatttat tacgtagaag cgaatgcgct tcgtcggtct ggtgttccag gctggggaac
     8821 gtttatcgac aaagaacttc aaggaggcgg gcctttgatc gattatggca ttcacatgct
     8881 tgatacagcg atgtatatta ctggatttcc taaagtaaaa agcgttcggg cgtacagctt
     8941 ccaaaaaatt ggtacgaaaa aaaattcagg gaaattcggg aaatgggatc caagcaaatt
     9001 cacagtagaa gatgcaattt ttggcacact ggaattagaa aacggcaaga tcatccaact
     9061 gaatacttcg tttgctttga atatcagaga agacaaactg aatgtggctt tttgtggcga
     9121 tcaggcaggt gctaatctgt atccaggcga actattcgaa gatgtagatg gaaagctggt
     9181 ttccttacaa aagaccacgc cagttaatga cccacatgaa ctaagtatgg ctcattttgt
     9241 gaatcgggcg ctcggtgacc agactcagat gattgcagat ggctggcaag gatatgtcat
     9301 ccagaatctg gtggaaaatt tatatcgttc ggcagaaaca ggagaagtta ttaccttatg
     9361 aaagtagaag aaaaacagtt tgatttagag tatatcaata agtatgcgag tcagatgacc
     9421 attggaaatg gattttttgg tatcagagga agccaggagg aagactatcg gaatcaagta
     9481 aggggaatgt ttgcagctgg tgtatataac cgacctttag gagcagaatc agccgagtta
     9541 gtgaatctcc cagatgtcat cagatgggaa ataaaaatcg acggtgaaca tttttctctt
     9601 gagacgatga aagttcttgc atacgagcgg gcactagatt tagaaatggg agagcttaaa
     9661 cgctatgcgc tcattgaaac tgctcaagga aaaaaagtgg aagtcattac ttgtcgtgtt
     9721 gctgatcaaa ataatctcca tcaggcagcc ctgaaagtgg tcatcaagcc gttgaccgaa
     9781 gactgccaag tagatgtgaa aacgggtatc gatggacagc aaaccaattt tggtacacag
     9841 cagttggttg aaaaagaact gcgagtattt ggtgacgatt tgatcagtgc atcctaccag
     9901 acaacagaaa gtaaaattga tattgggatt gcagctaaat tcaataaagc aggtgtattc
     9961 caagcaaaaa atcgccggat caccgcttcc ttttcagaaa agatcagaaa aaatacgtcc
    10021 tttacgctgg agaagatcat cgtgctctct acctctcttc agcaaacaga cccgtttaag
    10081 aaaagtattg cccaagccca gaaaagcgtt agttatacag aaattttaga gcagtcgatg
    10141 tcttattgga cagatttttg gcatcagcat cggataacga tccaaggcga ttcttctttc
    10201 gatcagttag cgatggactt cgctgcttac catttacaga ttatgactcc ccgcaatgat
    10261 ccaagtgtta gtgtaggggc gaagggacta actggcgaag gttataaagg gcatgttttc
    10321 tgggatacgg aaatttttat cctgcctttc tttttatatc agtttcctga agtagcaaag
    10381 aatttgctgc tgtaccgtta tgatcgatta gaaggcgcac ggcaaaaagc aagagattat
    10441 cactatcaag gtgcactttt cccatgggaa agcgcacaca ctggtcagga agagacaccc
    10501 aaatttgctg cattgaatat caaaaccgga acaagacaag aagttgcttc agctgccgca
    10561 gaacatcata tcagtgcaga ccttgcttat gccgttcaag agtatgtcca agcaacagag
    10621 gatcaatcgt ttttagaaac aaagggaaaa acgctcattc atgaaacagc agctttttgg
    10681 ctgagcagag ctgtaaaagt cgatgatcat ttagagatac acagagtcat tggtccagat
    10741 gaatacacag aatatatcga caataatgca tatacgaact atctggcgca ttataatgtg
    10801 gccttagctg ttaaatatca agctggcgat ccgctttttt tgaaagaatg tcaaaatttt
    10861 ttagataagt tatatttgcc agtgcctaac aaagaacagc tgattccgca agatgacacg
    10921 tatctcagta agccagttat tgatttgacc acttacaaag aacgtcctgg cagccagtct
    10981 attttacttg attatgcccg tagcgaagta aatgaactcc aagtcagcaa gcaagcagat
    11041 cttgtcatgc tgttatactt attacctgag tatttttctc aaccaatcgt ttctaagaat
    11101 ttgcattact atgaaaacaa aacaatccac gactcatcgt tgagtaaagc gattcacgcc
    11161 atagaggctt gccgtatcca agaaatggaa tgggcgtatc atttattcca agaagcgtgc
    11221 ctgattgatt taggaccaga tccgcattca tcagatgacg gaattcatgc tgcttcatta
    11281 ggagcactat ggctagcagt tatatttggt ttttcaggta ttacagtaac gaatggaact
    11341 ctttctattt ctccaagact tccttcagca tgggaaaaaa tttcttttcc ttttatttgg
    11401 aaaaatcaca ggttgctatt tgttttgaca aaaaaacaaa tagtaataaa aaaagagacg
    11461 aaagaaacat tacctatttt tgtcaatgga gaccaatacc agctggaaaa agaactgatc
    11521 ttggaacgga ggggaaaata atgcgtattc tgattgcgcc agattctttc aaagaaagtc
    11581 tgacagcctt agaggcagca caagccattc aatcaggttt tcaagaagtt tttgttcagg
    11641 ctgaatatga tctgctacct ataggcgatg gaggagaagg aacggtttca tcattagcag
    11701 acagtttaag gttgaagcgg caaacagcag tcgttactaa tgcatttggc aaaaacaaag
    11761 aaatccagta cgcttcagac ggtcaaactg cgatttttga aatggcagaa atcgtcggat
    11821 tgaaagatgt cccactaaac caacaacagc cacttaggtt atccacgcga ggagtaggag
    11881 aactgatacg acaattgttg caacaaggtg ttcgacacat catcatcggc gttggcggaa
    11941 gtagtaccaa cgatggcggc atcggcatgg cagaaggtct aggttatgct ttttacgatc
    12001 aagataatca aaaagtagaa gcactgggag aaaatctagg taaaattgct acagttgata
    12061 aaacgaacgt gcctattgag ctgattaaag ccaaaatcac tgtcattact gatgttacta
    12121 atcttttatg cggaaaagaa ggcgcttctt ttgttttcgg accacaaaaa ggcttgaaag
    12181 aaaatgaatt agcagtggct gaccaagcca tgcatgattt ttatatgaga tttgcccctc
    12241 aagtaatcga ggcatcgggc agtagtgctg gtggtggaat gggtgcagga ctgttagcat
    12301 ttgcttcagg gaaaatagtc tctggcatcg actacgtatt ggatttactg gagttcgata
    12361 aacgggtaca gcaagcagac ctcgtaatcg taggcgaagg acgtctagat ggacaaagtc
    12421 taaaaggaaa agcgccagtt gggattgcta gaagagtacc agagggcgtt cctgtgatag
    12481 ctatttgcgg aagtgttggt aaaggaagcg aaaaagcagc agatgttggc atttcggccg
    12541 tttttccgat ccttcctgca gctgtgcctt tacaagaagc tttacagcaa gccactgata
    12601 atcttcatcg cacagctaaa aacatcgcat ctttacttca agtcgttcat tttcaaggag
    12661 gtaatcgatg aaaatcaaag cagcactatt cgatttagat ggcgttcttg tagacacagc
    12721 tcgttatcat tacgaagcgt ggctggtttt agctaaccag ctgtccattc cattcactga
    12781 gaaagaaaac gagcaactga aaggaatatc cagaaccgag tcactagagc gattgctgtc
    12841 atttggaaaa atggaacaga aattctcaga aaaagagaaa tcagcctttg ctgagcaaaa
    12901 aaacaatcta tatcttcaag cgattcaaaa aatggatgaa acatccgttt tgcctggtgc
    12961 aatagctgtt cttgagtact tgaaaaaaac aaacatcaag atcggattgg gatcagcaag
    13021 caaaaatgca cgcctcattt tagaaaaaac gaatttgaca agttattttg atgtccttat
    13081 tgacggcacg caagtatcaa aagcaaaacc tgatccagaa gtttttctca aaggagcaca
    13141 gcaattgaat gtaccgccaa atgcatgctt ggtcattgaa gattcagaag ctggttgtca
    13201 agcagctttg gctggtaaca tgcatgtatt gggaataggt gaaaacataa atcttccttc
    13261 tgctgaatat gtgattcctg atttaaccgt ttttgatcaa gtacgttcgt tttggcattt
    13321 atcagaagcc gtatcacgta tataatagtt tccgtttttt ctgtcacaag caaaacgtta
    13381 tcctcctttt ggaaatcaag gtatgatgag aaaaaagggg gattttatat gacaaaaaaa
    13441 gcagttttcg caggcggatg cttttggtgt atggtcaaac catttgaaga gcagccaggg
    13501 atcatttctg tgacatccgg ttatactggc gggcatgtac caaatccaac ttatgaacaa
    13561 gtaacaacag gcacaacagg gcatacagaa gcagtagaga tcgaatatga tccagaagtt
    13621 atttcttatg atcagttggt ggaaatctat tggcagcaaa ccgatccgac tgacgcatta
    13681 ggacagttcg ctgatcgcgg tgattcttat cgtccagtga tctactataa tgatgaagaa
    13741 gaacgaaata ttgcagagcg ttcaaaacaa gcattgcaag acagcgggcg attcgataag
    13801 ccaatcgtga caaaaatcga accaagaagc gaattttatc ctgcagaaga ttaccatcag
    13861 gattattaca agaaaaacaa gctccattac aatctttatc gagaaggttc tggacgtgca
    13921 ggtttccttc gtcgacattg gggcgtcaag aaataagaag ccgaaaaagc tacttatgga
    13981 acaccgtata tcagaggttg taaaaccagc attttgagct aatactgagg gtcagctgat
    14041 tgaacaccat ttagtggaga ggttgtgaac ctgtcgccta gctccaagtc acaaggattt
    14101 ggtaagagaa cactatatat cagaggttgt gacaagtcgt ttgtcacaac cttttttcaa
    14161 tacctaagat gaaggacaat cagcaacgca ctgctcaaaa aaggaatgaa aggcagcttt
    14221 tccaactttt ttttatgaca gataaagtaa acagaaaaaa cggatattcc aaacagacta
    14281 gcaagaaaca agatctgaag caactcttct agagacaagc caccactcca agaaagcatc
    14341 aacagagtat cgccagctcc gagataggat tgaccatagc gaaggcacac accgcctacg
    14401 ataaacgtta acagcacact ttgccattgg aagacgccgg cctgcaacca aaaaatccat
    14461 aaaattgccc aagaacagaa tagcgtgtga gaatggacca tgaagtaaaa taaatccatc
    14521 aacgaaaata gcaaagccga aagcatccat atgaacgtcg aaaaatttct tgcggaagaa
    14581 aaagtaaaga accaggcaaa aagaccacca cataaggctt ctgccaacaa atagtagaag
    14641 ggcaaccgac acctgcaata gcggcagcga aatcgttgga cgatgatcga gagaatcgga
    14701 atcaattcat accaagaaag cgaacgacag catttgacac aatgagatct aggataaata
    14761 atagagtgac ctaacggaat ccgctgggcg gttaaacata aaaatgatcc gatacagcaa
    14821 ccaatgataa aataaagtaa cacgccatct cccccttttc aaaaccaaga tacgcaagct
    14881 taaactaaat ttttgtacat acataaaatt tgaggtgaaa aaatgaacgt ttcaatcgga
    14941 ttagatatcg gaaccacaca aaccaaagca gttgctttca cagatgaagc ccaagaagca
    15001 gcatccgctt attttcgcta ccctttgatc caagaaacac aagggatggc agaacaagat
    15061 caggaattga tctttcaagg tgtctgcacc gttataaaag aagtaattaa acagttgcct
    15121 tcagacacaa tcattcaatt tgtctccttc tcctctgcga tgcatggcct gatattgttg
    15181 gatgaagaga aaacaccgtt atctcgaatg atcacttggg cagataaccg agcagcggac
    15241 gaagcagaaa aactgaaata taccgtgctt ggcaaagaat tttatcggca gactgggacg
    15301 ccgattcatc caatgacacc attgaacaaa atcaagtggc tgcaaaacag ccaacctgaa
    15361 ttattcaaaa aaactgctta ttttgtcggt atcaaagact atatctttta tagattgttt
    15421 ggcgaactga tcagcgacta cggaacagct tcaggaactg gatattttga tatccatgaa
    15481 ttccaatggg cagaagaagt gcttgactac ttgaacattc gaatagagca attgccgcaa
    15541 gtctttcctt ccacctatca agtaacaggt ctaccatcaa aaacagcagc agaactaggt
    15601 ttgccaaatg atatatcatt tgttttggga ggggcagacg ggccgctttc taatctaggg
    15661 ttaggtgctt ttggtgatac gatcgccgct ttgaccgtag gaacgagcgg agcacttcgt
    15721 tttattacta ctcagcctca gttccatcca gaaatggaga cgttttgtta tgttctggat
    15781 cagaaacatt gggtagtggg aggagcgact agcaatggtg ctggcatctt tgattgggca
    15841 gggcatacat ttttacagga agtcgttcaa tctgctaaag aaaaaggaga aaatccatat
    15901 gatgcactgc tttcggctat cgcttttgtg ccagcgggtg ccaatggtct gatttttcat
    15961 ccctatttac ttggtgagcg agcaccgctt tgggacgcag atgcgtttgg cagttttatc
    16021 ggtttacagc ggcaacatac agaaaaagaa atgatgaagg cgattctaga agggatctgt
    16081 ctcaaccttt atcggatttt aaaaggagtt tgccaggaaa atcagctcac agagatccgt
    16141 gctacaggcg ggtttgctca atctccgatt tttcgtcaga tgatggctga tgtactgggt
    16201 tacccgcttg ttttccctac tgtcacagaa gcatccgcat taggagctgt gattttaggc
    16261 tggagaagca tcggaaagat ccaaagtatc agtgaagcac aggaattgat agaattgaaa
    16321 gagcaagttg gagtacaaga aactgatcat aaaaaatatc agaaaatcta tccattattt
    16381 gtttcgacac aaaagcattt agcacagact tatcagcaat ttgctcaact gcgagaagaa
    16441 ttagctgatt ctgaaaaatg attggcttta cggataaaaa agcgaactga aaatcgttct
    16501 caggtaaatg attttgttgt acttttcatt gaaagtctac tattatttta agtgtaagaa
    16561 aatgttagga ggcaagaaag atgaaattcc aacaaaccaa agaagtatta aatcaattag
    16621 ttgcagatct aagccaaatg tctgttgttg ttcaccaaac acattggtac atgagaggac
    16681 caggtttcct tacattgcac ccaatgatgg atgagttcat ggatgatttg aacgctcagt
    16741 tagatgaagt atccgaacgc ttgatcacat tagacggttc accttattct acattgcgtg
    16801 aatttgcaga taacacaaaa atccctgatg aaattggccg ctgggatcgt actattccag
    16861 aacgtttgga aacacttgtt gcaggctatc gttatctagc agatctttac caaaaaggaa
    16921 tcgaagtttc tggagaagaa ggcgacgatc caacacaaga tatctttatc gaattcaaaa
    16981 cagccacaga aaaacgtatc tggatgatcc aagctcattt gggcaaagcg ccagagatcg
    17041 acgcgtaaaa actgttgttg aacttgaccg aaaaagaggt tgtgaaatca gcgttttgac
    17101 ctgagtatta gagaaacaaa cgtcaaaata gttcctcata ttttgccgtt ttttgagcta
    17161 ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtaagcct gccgttcagc
    17221 tccgagtcac aagaaacaat tttaaaaaac agcttcttat gttttatgga tattgtgact
    17281 tgtgaccgag gagttggcag gagaacaccg tttattcaaa ataaggggtt gtgacaagac
    17341 ttttgtcaca gcctcttttt gtttttcatt ttgacaggaa acgacccttt tatataaaag
    17401 aaagaaaaga agtaaagttt catataaatt tttcaatcaa gcagaaaaac gttgacattg
    17461 actagattag cgggtaatat gttaaatggt attgtttgcc ccacaaagag ccccggaaac
    17521 tcgaagtgta tgtcaaacat cagaacttaa attggaggaa aataacgtgc gtacaacata
    17581 tatggccaaa ccaggcgaag tagaacgtaa atggtatgta gtagacgcaa ctgatgttcc
    17641 attgggacgt ctttcaacag ttgttgcatc tgtactacgt ggtaaaaata aaccaacttt
    17701 cacacctcat ttggatactg gcgatttcgt catcgtcatc aatgcagaca aagtgaaatt
    17761 aacaggtaaa aaagcgactg acaaaattta ttaccgtcac agcaactacc caggaggatt
    17821 gaaatcaatc actgctggcg aattgcgtgc taaaaattct cgtcgtttga tcgaaacttc
    17881 tgtaaaaggt atgcttccta aaaacacttt aggccgcaaa caatttacaa aattgaacgt
    17941 atacggtgga gcagaacatc cacatgcagc acaacaacca gaagttttag acatcacaaa
    18001 cttaatctaa taggagggaa tttcattggc acaagttcaa tatatcggca caggccgtcg
    18061 taaaaacgca gttgctcgcg tacgtctagt accaggaact ggcaaaatta ctgtaaataa
    18121 aaaagatgtt gaagaataca ttccacatgc tgacttgcgt gaagtaatca atcaaccttt
    18181 cgctgttact gaaacaaaag gcgcatatga cgtatttgtt aacgtaaacg gcggaggata
    18241 cgctggacaa tcaggagcta tccgtcatgg tatcgctcgt gcattgctag aagttgatcc
    18301 tgacttccgt ttagctctta aacgtgctgg cttacttact cgtgacgcac gtatggtcga
    18361 acgtaaaaaa ccaggtctta agaaagcacg taaagcttca caattctcaa aacgttaata
    18421 tcctttatat atcaacgttt ctggcacttt tcaattattt ggaaagtgct tcttttgttt
    18481 ttaagaacac atttaagaac acatatcaat cacacttatt caatcctctc tgaaaagctt
    18541 caaaaacagc cttatcaact atatctggtg tgttcacata tatctttgtt gtagaaatat
    18601 ctgagtgtgt taaagcattt gaaatttgat tcatatctgc tccaccttgt ctagctatag
    18661 ttgcataagt atgacggagt ttgtgtggag ataaatgaac taagtcagga tgccttcttt
    18721 taattgaatt gattcgatag tttaggtaat cggcatgaac aggacaattt acttctccag
    18781 atggtttagt ataagtaaag agatactgat ctaacgtttg cttaattttt aatttcaaca
    18841 attgatcagc ttgtaccgat ttccattcac ttagcaatgt cattacaact tctggtactt
    18901 gaatgacggt atcttttcga ccttttgtaa acttttttct ttgatattta tctaaagtat
    18961 gtttaagacg gaccgtttgt ttttcaaaat caatatattt ccattgcaaa gcgtaagtct
    19021 cactttttct atcaccaaga tacagggtaa tcataaaaag tgtgtaatca tgaaaagtaa
    19081 gtaaatgatt attaaaatca tcatttactg cttctatcca attagtaagt tcttttgatg
    19141 atagtgcttg cttaccttct tgtattcttt tttcttctaa agcatttttt ttaggtgcag
    19201 tgatactttg aataatttta gtagttcgat taaattcgat ataatttagt atttctgcaa
    19261 tatcgaacat ggaacgaaca tatcctttta tgatttttat atttgcatat tctttcgatt
    19321 ttttggttaa ttcatctgat attatctcag tattg
//
LOCUS       DASJUF010000051        19248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJUF010000051 DASJUF010000000
VERSION     DASJUF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.051"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(248..613)
                     /locus_tag="U4B62_001614"
     CDS             complement(248..613)
                     /locus_tag="U4B62_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141776.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            821..1303
                     /locus_tag="U4B62_001615"
     CDS             821..1303
                     /locus_tag="U4B62_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141777.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1500..2390
                     /locus_tag="U4B62_001616"
     CDS             1500..2390
                     /locus_tag="U4B62_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN2141778.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2715..5186
                     /locus_tag="U4B62_001617"
     CDS             2715..5186
                     /locus_tag="U4B62_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2141779.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5289..7025)
                     /locus_tag="U4B62_001618"
     CDS             complement(5289..7025)
                     /locus_tag="U4B62_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN2141780.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7022..7726)
                     /locus_tag="U4B62_001619"
     CDS             complement(7022..7726)
                     /locus_tag="U4B62_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2141781.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7857..8360
                     /locus_tag="U4B62_001620"
     CDS             7857..8360
                     /locus_tag="U4B62_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN2141782.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8320..8658
                     /locus_tag="U4B62_001621"
     CDS             8320..8658
                     /locus_tag="U4B62_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN2141783.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8679..10097
                     /gene="ffh"
                     /locus_tag="U4B62_001622"
     CDS             8679..10097
                     /gene="ffh"
                     /locus_tag="U4B62_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN2141784.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10209..10787
                     /locus_tag="U4B62_001623"
     CDS             10209..10787
                     /locus_tag="U4B62_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN2141785.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10791..11312
                     /locus_tag="U4B62_001624"
     CDS             10791..11312
                     /locus_tag="U4B62_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2141786.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11391..11945)
                     /locus_tag="U4B62_001625"
     CDS             complement(11391..11945)
                     /locus_tag="U4B62_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2141787.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12198..12473
                     /gene="rpsP"
                     /locus_tag="U4B62_001626"
     CDS             12198..12473
                     /gene="rpsP"
                     /locus_tag="U4B62_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN2141788.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12485..12736
                     /locus_tag="U4B62_001627"
     CDS             12485..12736
                     /locus_tag="U4B62_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN2141789.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12861..13652
                     /locus_tag="U4B62_001628"
     CDS             12861..13652
                     /locus_tag="U4B62_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141790.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13822..14343
                     /gene="rimM"
                     /locus_tag="U4B62_001629"
     CDS             13822..14343
                     /gene="rimM"
                     /locus_tag="U4B62_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN2141791.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14333..15094
                     /gene="trmD"
                     /locus_tag="U4B62_001630"
     CDS             14333..15094
                     /gene="trmD"
                     /locus_tag="U4B62_001630"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN2141792.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15230..15577
                     /gene="rplS"
                     /locus_tag="U4B62_001631"
     CDS             15230..15577
                     /gene="rplS"
                     /locus_tag="U4B62_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN2141793.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15709..16845)
                     /locus_tag="U4B62_001632"
     CDS             complement(15709..16845)
                     /locus_tag="U4B62_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2141794.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(16961..17527)
                     /locus_tag="U4B62_001633"
     CDS             complement(16961..17527)
                     /locus_tag="U4B62_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEN2141795.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(17663..18316)
                     /locus_tag="U4B62_001634"
     CDS             complement(17663..18316)
                     /locus_tag="U4B62_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141796.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18366..18794)
                     /locus_tag="U4B62_001635"
     CDS             complement(18366..18794)
                     /locus_tag="U4B62_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2141797.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(18799..19173)
                     /locus_tag="U4B62_001636"
     CDS             complement(18799..19173)
                     /locus_tag="U4B62_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2141798.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
BASE COUNT         6209 a         3464 c         3795 g         5780 t
ORIGIN      
        1 aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac tagtgatagt
       61 tttggtgata gtgatattaa aaatcagaaa agatagaaaa agcaggagat agataaaatg
      121 ctgatatttc agcatttttt aagagtataa aagagaaaaa aagtaataaa tgtatacatg
      181 atataagaaa aacatatatg tataaaaaga agaaagtagc ttctctttgt acatatatgg
      241 tttgttttta tggatgccga gcttttttat aagataaggc taccgcccat aacgacccaa
      301 ggatttgtgg aactaagtaa atacctgcaa gaagcaagat cagctgggtc ggttttaaag
      361 gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa taaataggaa gtgacagtag
      421 atataatatt ggcaatgaat aaagatataa tacgcctgtt cctttgaaaa taatatccag
      481 caaaaagaga taaaaatatc aatgtgatga acccgcttac ggaatgataa cgaaaatcaa
      541 gcagtaaggc gaaatgcaca taaggaacaa aagataaaag caacggaagg tttttccagc
      601 aagtttttat catctttcat caccacctaa caagtgttat tattttgtta gttttattgt
      661 aaattgaatc ctgcaataat caattacttt ttcattattt ctagattaaa tgaataaact
      721 ctcatgccat tatctctaaa aaaaaagttt ttccccctaa tttttttaga tagaaaattg
      781 tatagtatga tgagagaaaa agtagtttgg gggaacgtta atgagaagaa aagcagtaat
      841 cgatcggctt tatttatttt tttggttttt tcctatcgtt ttgctacctt ttacatgggt
      901 aaatgctgtt gtacaaggtg cggagatatt aggcctgatc caaggaagcg gatttgtggt
      961 ttctaggaat tttccagctt tgactgtttt ctatttagtg ataagtgtag gtctcttatt
     1021 accaaaatct ctgatcaatc actacaattg gcagctgctt tacttttctt gcttattagt
     1081 atttacatct atgttttctt tgctgcctcg cttgattatc ggccctaatg gtttgagcga
     1141 gagtggattg aaggattcag cttattcctc catgtatttc ttgacgatca aaccatggtt
     1201 ctatttagta attgcctcat ttattcttgc aacatttttt tacttctgga cagaaatcag
     1261 gataaaaagt gaacgatctg tatctataaa aaataactat tgatggtgga tcatcagtaa
     1321 aaaagcatat gagctgcgta aaatgcatgt ttctcatatg ctttttttat tattatgtaa
     1381 agaatgcgaa aaacgtttac atgattttta cataacttaa ctatattttt acagcaacca
     1441 tacgagggat ttacagtcga ctgataaact ttagttgtaa ataagaaagg agcaataaaa
     1501 tgaaaaaagt attgtttact ctaggtatga tgggaattct tttagccgga tgcggctcag
     1561 gatcaggaaa tacagatggc tctgccacac aaaataattc aagtgactca aatgaacaag
     1621 taaaaatcgt tgcggtaggt tcgacagcat tacagccgtt agtcgatgca gcacaagaaa
     1681 gctttgtaca agaaaatcca aattatcaaa tctctgtaca aggaggagga agcggaactg
     1741 gtttgagcca agtcgaagct ggagcagtta ctatcggaaa ctctgatgta tttgcagaag
     1801 aaagagacgg agttgatgct tcaaaattag tggatcataa agtagcagtg gtcgggatgg
     1861 cgccaatcgt caataaagat acagatgtca aagatatcac caaacaggaa ttgattgata
     1921 tctttacagg aaaaatcact aactggaagg aagttggcgg gaaagatcaa aaaatcaacg
     1981 tcgtgaaccg tgcaaacggc agcggtaccc gcgcgacatt cgaaaaatgg ggactggatg
     2041 gtgctacccc tgtgcagtcc caagaacaag attcatcagg aacagttcgg cagctggtaa
     2101 gtcaaacacc aggagcaatc agctatttag ctttctcata tctcgatgat tccacacaag
     2161 cactaagtat cgacggtgta gaaccaaaag aagagaatgt agcagataac agctggggaa
     2221 tctggtcata tgaacatatg tacacgaacg gaaaaccttc ccctgaagtc caaaaattct
     2281 tagactacat gatgacagaa gaaatccaag aaggacctgt caaagaattg ggctatttac
     2341 caatcacaat gatggaagta gaacgtgatc acgaaggaaa tatcaaataa ctagcagata
     2401 aacatgccac ggtagtcgtt actattaagg aaaaatcaac tatgtttccc acatatacat
     2461 ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag ctaataccga
     2521 gggtcagctg attaaacacc gtttattcga aaaaaagagc tacgacatag aactgtcgta
     2581 gctctttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
     2641 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
     2701 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
     2761 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
     2821 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
     2881 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
     2941 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
     3001 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
     3061 aactaaatgt tttaaataca gcaaaagtca atgtcatacg ggacgggata gaacaaacaa
     3121 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga catcaaattc
     3181 cagcagatgc agaggtcgtg gaaggaaaac tgcaagttaa tgaggcttta ttgacaggcg
     3241 aagcagatga aataaccaaa gaaataggcg atccattaat gtccggcagt ttcattgttt
     3301 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
     3361 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
     3421 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
     3481 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
     3541 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     3601 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     3661 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     3721 aaaaagtgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     3781 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     3841 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     3901 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     3961 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     4021 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     4081 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     4141 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     4201 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     4261 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     4321 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     4381 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     4441 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     4501 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     4561 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     4621 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     4681 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     4741 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     4801 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     4861 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     4921 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     4981 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     5041 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     5101 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     5161 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     5221 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     5281 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     5341 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     5401 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     5461 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     5521 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     5581 tcaatcagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     5641 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     5701 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     5761 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     5821 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     5881 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     5941 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     6001 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     6061 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     6121 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     6181 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     6241 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     6301 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     6361 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     6421 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     6481 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     6541 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     6601 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     6661 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     6721 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     6781 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     6841 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     6901 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     6961 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     7021 ttcatcgttt tggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     7081 gatgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     7141 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     7201 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     7261 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     7321 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatc cgtctgaata
     7381 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     7441 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     7501 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     7561 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     7621 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     7681 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     7741 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     7801 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     7861 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     7921 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     7981 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     8041 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     8101 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     8161 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     8221 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     8281 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     8341 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     8401 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     8461 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     8521 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     8581 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     8641 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     8701 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     8761 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     8821 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     8881 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     8941 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     9001 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     9061 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     9121 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     9181 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     9241 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     9301 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     9361 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     9421 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     9481 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     9541 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     9601 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     9661 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     9721 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     9781 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     9841 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     9901 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     9961 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
    10021 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
    10081 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
    10141 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
    10201 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
    10261 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
    10321 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
    10381 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
    10441 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
    10501 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
    10561 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
    10621 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
    10681 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
    10741 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
    10801 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
    10861 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
    10921 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
    10981 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
    11041 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
    11101 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
    11161 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
    11221 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
    11281 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
    11341 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
    11401 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
    11461 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
    11521 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
    11581 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
    11641 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
    11701 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
    11761 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
    11821 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
    11881 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
    11941 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
    12001 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
    12061 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
    12121 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
    12181 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
    12241 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
    12301 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
    12361 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
    12421 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
    12481 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
    12541 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    12601 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    12661 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    12721 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    12781 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    12841 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    12901 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    12961 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    13021 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    13081 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    13141 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    13201 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    13261 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    13321 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    13381 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    13441 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    13501 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    13561 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    13621 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    13681 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    13741 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    13801 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    13861 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    13921 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    13981 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    14041 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    14101 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    14161 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    14221 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    14281 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    14341 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    14401 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    14461 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    14521 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    14581 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    14641 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    14701 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    14761 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    14821 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    14881 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    14941 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    15001 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    15061 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    15121 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    15181 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    15241 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    15301 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    15361 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    15421 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    15481 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    15541 cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag acagagaaag
    15601 aacccttggt attcaagggt tcttttttta ttatctgttt ttttatatta acgatttcta
    15661 tttggtacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt acagtttttc
    15721 tatatcttct ttcaattttt cttttactcc ttttgtcaca tgcaaataaa ttttttcagt
    15781 gatatcacta ttttcgtgac caacgcgatc ctgaatggca tacagaggag ttcctagttc
    15841 cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt
    15901 catgtcagct ttatgacttc tcaaataggt gttaattgct gttagttgga aaggagttcc
    15961 tttcgttgtt tgaaatagaa attgaccatt tggatttaat tctagaagct cattgtaaat
    16021 agctatcgct ttttttggta aatcaatttc tcgcattccg gcagctgttt ttgtagaatc
    16081 agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat ttataactac
    16141 tgatgcatta ttattggtaa tgtgtacatc atctttactt aatgcgattg cttcaccaga
    16201 cctcatccca gtcaaatata gccactggaa aaggagagaa tatcttttat tgtgtgaagt
    16261 tgtgaaatct actagtctat tatattcatc atcttctaaa aatttatctt taatttttat
    16321 cgtttttgac tctcttttat aatcaataac tacttcatcg ataggatttt tttctacata
    16381 accttttttc atagcatatg aaaaaagaag attcaatttg gatttaatta cactgacgta
    16441 cttatttgac aagtcatctt tatatatcat gtcttcaaaa atattattca aatctattgt
    16501 attaatacca gaaacgatgt aagtctcagg tatcttcttc tttatagtgt ttaatatatt
    16561 attcgtagga taatatgtag attccttaac ttgtcttttg tatataacta accattcttc
    16621 aacaagctca tgaaatgtta gatcaggctt atgaagtgtt ttttcgttta attttatatc
    16681 aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt tatttttata
    16741 agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct cattatattt
    16801 atatactttt tttccgtttt tatcagtttt agtttcgatc cacattgttt tcatctccta
    16861 tttgttgcta taataggaat agataagtaa gccgattata gcaggtttat ttttcatcac
    16921 gtccacaaac tttggcgagg gaggggcgtg tttttgttag ttattgtggt aggttatcta
    16981 aagcgtattg tgcttgctca tttgtaaaac cttcaaatat aagttgatcg tacaattgcg
    17041 aatctgacat tgatgcccaa tcatcgtaac tttccgcttt agccaaagct tgttcgttgt
    17101 aatcgacatc aacattagcc agcgcataat cgattgcatc acttggatat tcttcaaact
    17161 ctagttgttc acgcaaacct tgttcagaca taccagccca acctagatag ctttcagctt
    17221 tgctcaatgc atttctgtat tcacgaggaa cactttcttc ttgagtaact tcttctatag
    17281 tagtttcagt agtttcagtt tcttcttcaa cgactgtaga ttctgctacc gattctgcag
    17341 aagacacttc tgtcacgctt tctttagttg tttcagcgac ggtattttta gcatcatcac
    17401 tgcctccttg cgaacctata gcaataaaca ccacaacaac cgctaacaac caaaaccata
    17461 cccgtttata aaacggtttc ttaactttat acattttccc atcttgaccc ataacttttt
    17521 ttgccattta aatatccctc gcttcttgtt ataatatatt tgtgatctca gaaatgaggt
    17581 atgagtccgt gttgcagcac gggctttttt ctttataact tttttagaga ttataggcaa
    17641 aatagtaggg cataaaaatg tattattgaa ttccgtattt agaaaatcct aattgaactt
    17701 caccggaagt cttttgctgt gttgtacgca atgcttcttc ttcagacatt ccattctgta
    17761 ctttccatgc aacaggcgac atcccgtatt tgttaacaaa atcagtaagt gataaagtgt
    17821 cagcgtcttg ctgagcgctt gtttgttggt cttctggatt ctgttgagat gctgcttgtt
    17881 gttctttctg atcttgactg ataatattgc cagcatcatc tgtagtcaat ccattttcat
    17941 aaagggccac gccgaaagct tcccactctt tgttggacca atttgcacga tcagctggag
    18001 ttgactgtaa agtgcgttgt ttcatctgtt catatgtttc ttcttgaggt gcggtttgga
    18061 ttgtactctg actggagctt ataactgttg ggctaggttc cgccgtagct tggttggagc
    18121 ttgtaactgt tgaactagaa tctgttttag atgtagattt gctagtagat gaactggttt
    18181 cagttgtttc ttttgtttta cttatttttg tttcttggtt agaagtggta tctgttgatt
    18241 cagctttttt attatttgaa caagctgaaa gtagcagagc agtacttaac aacaacataa
    18301 cgctaacttt tttcatttta taattcctct ttctcgttgt aatatgtgtg ctaacacggg
    18361 cttttttata taagaaaacg ataagcgctt tctggaagcc cgtaaagatt ctttaattcc
    18421 tcgatttttt taggatattg atcattatct tctttataaa gagaaacaat gagattagca
    18481 gcaaagcaat tagcttcgct ttcagatttg cttctagatg ttcttgttga tacataatag
    18541 ctggataagc cacgatgaaa aatagcgtga cctaattcgt gagcgcaaat gtagaatctt
    18601 tcctcagagt ctcgcagttc atcatttaaa aagattatcg cacgatctct aatttcttga
    18661 aactgcccct tgggattctc gataaaagga acgtattgaa ttttaatgcc catcttttca
    18721 caaatataaa aaggattagc ggactggtat ttccgcttca actcctcgac taaattaatc
    18781 gtatccatct ccataagctc acatcttttt gcctttttct ttgtcttctt tcacaatatc
    18841 ccagaaagtc gctatcagga tatcttttac gcgctgtatt tgttcgggtg tcaatgtttc
    18901 cccaccataa gacatattaa catttgagtc tagtagttta tcaagttcaa ccacttcctc
    18961 ttttgtagcc cacttgggaa cattattatt tcccaataaa taatcagttg tgacaccaaa
    19021 ataatcagca actttcttta agttctcaga ttttggcgag gctttatccc atcttcttat
    19081 ttgtccatta gagatgccca cctgtctttc tacttctgct atagtcacat gcttttcgtc
    19141 tgctaattct ttaatcttag taactaaact cattattatc aacctttcaa agctgaaaag
    19201 aaaataaata gcttaaaagt tatatttttg gttgacaatt agctttta
//
LOCUS       DASJUF010000052        18772 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJUF010000052 DASJUF010000000
VERSION     DASJUF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.052"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..1448
                     /locus_tag="U4B62_001637"
     CDS             21..1448
                     /locus_tag="U4B62_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141799.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="U4B62_001638"
     CDS             complement(1540..1905)
                     /locus_tag="U4B62_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2141800.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="U4B62_001639"
     CDS             complement(1902..2267)
                     /locus_tag="U4B62_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2141801.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="U4B62_001640"
     CDS             complement(2459..2707)
                     /locus_tag="U4B62_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141802.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="U4B62_001641"
     CDS             2706..2834
                     /locus_tag="U4B62_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN2141803.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="U4B62_001642"
     CDS             2891..4585
                     /locus_tag="U4B62_001642"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN2141804.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4589..4708
                     /locus_tag="U4B62_001643"
                     /pseudo
     CDS             4589..4708
                     /locus_tag="U4B62_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4685..4858
                     /locus_tag="U4B62_001644"
     CDS             4685..4858
                     /locus_tag="U4B62_001644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141805.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="U4B62_001645"
     CDS             5236..6369
                     /locus_tag="U4B62_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN2141806.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="U4B62_001646"
     CDS             6400..6720
                     /locus_tag="U4B62_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN2141807.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="U4B62_001647"
     CDS             complement(6749..7213)
                     /locus_tag="U4B62_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2141808.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7345..8598)
                     /locus_tag="U4B62_001648"
     CDS             complement(7345..8598)
                     /locus_tag="U4B62_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2141809.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8627..8944)
                     /locus_tag="U4B62_001649"
     CDS             complement(8627..8944)
                     /locus_tag="U4B62_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2141810.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8970..9290)
                     /locus_tag="U4B62_001650"
     CDS             complement(8970..9290)
                     /locus_tag="U4B62_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2141811.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9754..11169)
                     /locus_tag="U4B62_001651"
     CDS             complement(9754..11169)
                     /locus_tag="U4B62_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2141812.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11186..12502)
                     /locus_tag="U4B62_001652"
     CDS             complement(11186..12502)
                     /locus_tag="U4B62_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2141813.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12652..13350)
                     /locus_tag="U4B62_001653"
     CDS             complement(12652..13350)
                     /locus_tag="U4B62_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN2141814.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13639..14595)
                     /locus_tag="U4B62_001654"
     CDS             complement(13639..14595)
                     /locus_tag="U4B62_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2141815.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="U4B62_001655"
     CDS             complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="U4B62_001655"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2141816.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15716..17350)
                     /locus_tag="U4B62_001656"
     CDS             complement(15716..17350)
                     /locus_tag="U4B62_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141817.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17337..18737)
                     /locus_tag="U4B62_001657"
     CDS             complement(17337..18737)
                     /locus_tag="U4B62_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141818.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
BASE COUNT         5938 a         3700 c         3137 g         5997 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
     7321 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
     7381 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
     7441 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
     7501 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
     7561 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
     7621 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
     7681 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     7741 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     7801 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     7861 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     7921 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     7981 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     8041 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     8101 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     8161 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     8221 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     8281 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     8341 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     8401 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     8461 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     8521 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     8581 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     8641 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     8701 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     8761 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     8821 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     8881 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     8941 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     9001 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     9061 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     9121 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     9181 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     9241 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     9301 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     9361 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     9421 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     9481 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
     9541 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
     9601 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
     9661 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
     9721 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
     9781 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
     9841 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
     9901 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
     9961 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    10021 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    10081 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    10141 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    10201 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    10261 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    10321 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    10381 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    10441 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    10501 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    10561 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    10621 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    10681 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    10741 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    10801 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    10861 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    10921 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    10981 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    11041 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    11101 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    11161 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    11221 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    11281 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    11341 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    11401 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    11461 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    11521 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    11581 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    11641 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    11701 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    11761 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    11821 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    11881 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    11941 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    12001 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    12061 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    12121 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    12181 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    12241 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    12301 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    12361 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    12421 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    12481 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    12541 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    12601 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    12661 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    12721 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    12781 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    12841 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    12901 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    12961 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    13021 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    13081 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    13141 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    13201 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    13261 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    13321 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    13381 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    13441 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    13501 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    13561 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    13621 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    13681 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    13741 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    13801 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    13861 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    13921 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    13981 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    14041 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    14101 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    14161 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    14221 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    14281 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    14341 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    14401 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    14461 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    14521 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    14581 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    14641 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    14701 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    14761 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    14821 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    14881 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    14941 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    15001 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    15061 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    15121 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    15181 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    15241 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    15301 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    15361 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    15421 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    15481 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    15541 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    15601 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    15661 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    15721 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    15781 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    15841 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    15901 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
    15961 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
    16021 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
    16081 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
    16141 caacaatatg tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag
    16201 ttaaagtgat taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt
    16261 atcttctatt tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc
    16321 ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg
    16381 gcaccaaact agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta
    16441 tgtttgttaa ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc
    16501 ccattgagta agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag
    16561 atgcatgagc atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt
    16621 caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata
    16681 attttttaaa tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa
    16741 aaataaaaat gataattaat gctgtactca ttaatgttga tagtacatgg ctgtacacta
    16801 gcaaactcat actcaatgct agctttacag acggatactt ttcttctttt ccagagatga
    16861 tttgataagc ataaaataat acagggataa caagtgttat tgcgatagct tcggcaattg
    16921 cggaacgata gtagatgtct actgtccgat aagcagagaa aatatagaga caactaaata
    16981 aaaatgccgc ttttttttgt tttaaaaacc gctcgccata atgataagct acgagtgttg
    17041 tacacaagtt caataaccaa acgtaaataa tatagcttac aatcaaattt tttgtcatcc
    17101 aatataacat tacagctgga aaaaaagtca gaaagggata aaaataattt acaccatatc
    17161 cagtataatt gaatgtctca taattaatcg gactggtaaa aatagtcgat aaccccttga
    17221 ttcgagataa gtgaaaagaa agatctccag tggtataaat actctgtgta tttttgaaaa
    17281 agatgaaaga atatatacta ctaactataa ttaatccact caatattact acttttttat
    17341 ttattttcat tcttcttgtt tatctccttt ttagtcacat atcgagaatc tttcaactta
    17401 ttaggtgcaa atcgtttaaa acttgaatac accactaaac aataagtcag aatactgatc
    17461 cataaagcca tgagagcagt tggagtatat tttgttgtaa tttctatctt attttctcca
    17521 gcatttacat ccacattcac taagccatgt tttttataag agtcatttac gactttaccg
    17581 tttactttta cctgtgtacc taaataagca tatactggta ctgtcacttt ttgctttttg
    17641 ttgcttgcca ctgtgtaagt aatgttatta tctgcagctt tatacttccc tttttcccac
    17701 gattctccta cttttacttc atgtttttct aagtgaggcc tttcttttat cgcttctgtt
    17761 ggggcgtaat cgtataatcc tcccatatct gatgttgtat aacgtgggag tgtttcttca
    17821 ttgataacaa tatgaccgtg ttttgattgg ataacttttg cgtaattttc ttttgctgct
    17881 atagacgtga cgagtagaaa caccacagta gcgccaatga ttaaccatga atatttttta
    17941 ttcatagtct ttttcataaa aacaagcgtt atgatcattg aagaaaaaaa gatttggata
    18001 cctaagatac gccaaggaaa ctggagtaag cttatcggtg ttttttgaaa caaaaaccat
    18061 ggaaataaat tagttacgca tacccatgta gaaactacgc caacatagat gattttttct
    18121 tttttctcca tttctttcag tatgaataaa ggaaaaatca acgcaaccaa tcctaataac
    18181 cccatagtgt atgttgtaat atcgttattt aacgcttgaa tgatactatc tgaaatagaa
    18241 agtgcttcta gctgcaaatc tgttttgaag ggactgtgta tttcttgata attcatctgc
    18301 tgcaacatag gataccaaaa atagctagtt aatgcacaag tcaaaaaaac agcctttact
    18361 aaattcacga ccacaaattt tatatcgttt tttatttgct tgcttaaaaa caaacgaact
    18421 ataatcatac aagcaatcaa tgcactacac aatagtgtag ttaagacatg agtatacaca
    18481 agtagactta tactgacaga taaagcgatg actttttttt ctctattgaa tatcaatcca
    18541 taagcataat ataaaacagc tgggataatc gtaagagcga ttgcttctgc aattgcggat
    18601 cgataataga tatcaactgt tcgataagtt gcaaaagtat ataaacaact aaataaaaaa
    18661 gcagcatcta tctttttaaa aaacttttct ccataataaa aactaatcag aatcgtacaa
    18721 atatttaata accaaatata tagaacataa cttaatatta agttatttga aa
//
LOCUS       DASJUF010000053        18242 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJUF010000053 DASJUF010000000
VERSION     DASJUF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.053"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(67..891)
                     /locus_tag="U4B62_001658"
     CDS             complement(67..891)
                     /locus_tag="U4B62_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2141819.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(864..1544)
                     /locus_tag="U4B62_001659"
     CDS             complement(864..1544)
                     /locus_tag="U4B62_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2141820.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1567..2505)
                     /locus_tag="U4B62_001660"
     CDS             complement(1567..2505)
                     /locus_tag="U4B62_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2141821.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2750..3613)
                     /locus_tag="U4B62_001661"
     CDS             complement(2750..3613)
                     /locus_tag="U4B62_001661"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN2141822.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3755..6328)
                     /gene="mprF"
                     /locus_tag="U4B62_001662"
     CDS             complement(3755..6328)
                     /gene="mprF"
                     /locus_tag="U4B62_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2141823.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6469..7275
                     /gene="proC"
                     /locus_tag="U4B62_001663"
     CDS             6469..7275
                     /gene="proC"
                     /locus_tag="U4B62_001663"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN2141824.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7354..8910)
                     /gene="rny"
                     /locus_tag="U4B62_001664"
     CDS             complement(7354..8910)
                     /gene="rny"
                     /locus_tag="U4B62_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN2141825.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9321..10370)
                     /gene="recA"
                     /locus_tag="U4B62_001665"
     CDS             complement(9321..10370)
                     /gene="recA"
                     /locus_tag="U4B62_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN2141826.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10479..11720)
                     /locus_tag="U4B62_001666"
     CDS             complement(10479..11720)
                     /locus_tag="U4B62_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN2141827.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11960..12538)
                     /gene="pgsA"
                     /locus_tag="U4B62_001667"
     CDS             complement(11960..12538)
                     /gene="pgsA"
                     /locus_tag="U4B62_001667"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN2141828.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12747..13670)
                     /locus_tag="U4B62_001668"
     CDS             complement(12747..13670)
                     /locus_tag="U4B62_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2141829.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14409..15695)
                     /locus_tag="U4B62_001669"
     CDS             complement(14409..15695)
                     /locus_tag="U4B62_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2141830.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15688..16956)
                     /locus_tag="U4B62_001670"
     CDS             complement(15688..16956)
                     /locus_tag="U4B62_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2141831.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17208..17669
                     /gene="mscL"
                     /locus_tag="U4B62_001671"
     CDS             17208..17669
                     /gene="mscL"
                     /locus_tag="U4B62_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN2141832.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17728..18174)
                     /locus_tag="U4B62_001672"
     CDS             complement(17728..18174)
                     /locus_tag="U4B62_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2141833.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         5212 a         3982 c         3183 g         5865 t
ORIGIN      
        1 tttattaaaa taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt
       61 tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat
      121 caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa
      181 cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac
      241 gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga
      301 tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt
      361 gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa
      421 agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa
      481 gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga
      541 ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa
      601 accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag
      661 atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag
      721 gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat
      781 cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa
      841 gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc
      901 tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg
      961 gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat
     1021 tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg
     1081 tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct
     1141 attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga
     1201 tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact
     1261 aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa
     1321 ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa
     1381 ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca
     1441 gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca
     1501 tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc
     1561 taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata
     1621 attttcgcct gctttgattt gttcattagt caagctttct aaaggattca acacttctag
     1681 cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa
     1741 ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg
     1801 tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt
     1861 cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag
     1921 ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt
     1981 cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac
     2041 ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc
     2101 atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc
     2161 tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac
     2221 aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct
     2281 ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac
     2341 gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac
     2401 gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag
     2461 taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc
     2521 aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa
     2581 cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt
     2641 aaaatggatg agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata
     2701 ccattttagg attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg
     2761 ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc
     2821 ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat
     2881 ctgctccgta cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg
     2941 ttatatcttc taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga
     3001 tggcttcagc aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa
     3061 acactgtacg tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca
     3121 tagaaggtaa tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga
     3181 cacagtaagg aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac
     3241 agcccagatt ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc
     3301 cgcctaatcc agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga
     3361 tattgtattt attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg
     3421 gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata
     3481 gatgatctgc caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc
     3541 gtctatataa tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca
     3601 tcctctcacc cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag
     3661 tatagcttat gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt
     3721 ttctcttcat aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg
     3781 ccaaactcgg aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa
     3841 atccgaaatt acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt
     3901 ttaatttctt ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga
     3961 tatgcatgac tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag
     4021 ctaagcctag attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta
     4081 acagatagtc agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg
     4141 cctgcttctt cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga
     4201 caataccgac accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt
     4261 tttctcgtgc accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt
     4321 ccggcagttg atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa
     4381 gttgctgact cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta
     4441 ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat
     4501 aaaacgcaag ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc
     4561 gcatcatttt aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct
     4621 gataaccaaa aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg
     4681 acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac
     4741 ggtctttttc gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg
     4801 ccactaaagc tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg
     4861 aaggaaacaa aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat
     4921 agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag
     4981 ctccccaaga ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta
     5041 acagaatcaa gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta
     5101 aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa
     5161 gtgctctagc aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa
     5221 aattgaatga gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga
     5281 ttgttgatag aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat
     5341 gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc
     5401 ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc
     5461 gataatataa tagccaaact actgctacat cttgccgaac agacaattga ctcattccta
     5521 agatcatcaa aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca
     5581 atgtagcaat catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact
     5641 tgccgatgat caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca
     5701 gaccaagcaa gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa
     5761 aaaagaaaat gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa
     5821 aaatactttc tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca
     5881 aaccagtcag cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg
     5941 tctgctttcc ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga
     6001 gattgttgat cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg
     6061 gttttccttg tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga
     6121 cacccagcag tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc
     6181 tttgccaaat atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa
     6241 agactaacac ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt
     6301 gcagcatcaa gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg
     6361 tcatacccca atgttatctt atttatttta atttttccac tttagacctg ttctttgctt
     6421 gcgatgatag aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga
     6481 tttatcggca cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca
     6541 gtctcgaaag aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct
     6601 gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc
     6661 gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat
     6721 agccagaaag tcttattcat ttctattgct tcaggaaaat cattaataga tttagaaaca
     6781 gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta
     6841 ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa
     6901 gctacgatga tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt
     6961 acattcattg gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg
     7021 ggacggactg gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa
     7081 gcaattatgg gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta
     7141 gaccaagttt cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca
     7201 ggattcatcc cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg
     7261 ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg
     7321 gcttttgccg cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt
     7381 ttcccgaata acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt
     7441 acgaatatca cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat
     7501 tacccgaact tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt
     7561 cgaaatattt tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc
     7621 accaggtctt gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt
     7681 agcttccaca tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta
     7741 cttggcagct aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc
     7801 tttcccaata tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc
     7861 tgctgctaag ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc
     7921 ataactagtt ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg
     7981 agcaccgact tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt
     8041 acgagatttc tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag
     8101 tttttccaag gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac
     8161 tgcttccggt gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat
     8221 attacgacct tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac
     8281 tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa
     8341 gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag
     8401 agttaactca tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc
     8461 ttttgataaa ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac
     8521 gtctttttct cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc
     8581 aagtgagttc tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg
     8641 aatcaaacga ttctcttgag attttaattc taacttactt tctctcagct cactttcaac
     8701 ttcagaacgg tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt
     8761 ttctgcttct ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat
     8821 ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc
     8881 gatgatagcg aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt
     8941 tttatcatct atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc
     9001 atatatgtgt acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa
     9061 ctttaaataa acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa
     9121 atgacactag tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc
     9181 tttgtagatg aacagatatt tttattttat gtacccactc gtataattaa ataactcacg
     9241 atttctttat tcaataaaaa aacggcaggt caaggagagt acctactctt cagaccagcc
     9301 gtttttgttt atcataattt ttattcttct actaaaggca attcttcttg tcctttttca
     9361 tcttctggga cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact
     9421 tctgccatca tttcaggatg attagccata tagattttag cgttttcccg tccttgacca
     9481 atccgatctt ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact
     9541 gccatgtcta gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca
     9601 gcgattttga aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca
     9661 acaatatctg taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg
     9721 tagaatttca atgcgcgtcc tccaggagtg atttctggat tcgcaaacat cacgcctact
     9781 ttttcacgga tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag
     9841 agtttacgca atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt
     9901 tcgccgtcga tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct
     9961 acagccccac ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc
    10021 tgtgaaagaa gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac
    10081 gcatgctcag catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg
    10141 tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca
    10201 cgggggtaac cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta
    10261 gagatttgtt ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt
    10321 tcaatttttt tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc
    10381 ctccttatat ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga
    10441 aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt
    10501 ctcaaaagat caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca
    10561 aagtgatatt tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct
    10621 gcagattgac cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct
    10681 gttcttgcta tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt
    10741 ccatattctg ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag
    10801 taggtaacga atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct
    10861 tggaaggcgc cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt
    10921 agttccacta cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct
    10981 actcgttctt ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca
    11041 tttgttttca ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt
    11101 ggattgatct gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc
    11161 ccataaaagc gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt
    11221 aaaggacgta cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg
    11281 atatacgcat gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt
    11341 agaggaaccc cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga
    11401 tgggttgttt caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac
    11461 ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg
    11521 ccacataaga gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc
    11581 tgctgattat ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt
    11641 tctgacaaga aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca
    11701 gctattattt ctgctttcat tacttcccgc tcccttatat agtatacgct gtatttttat
    11761 atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt
    11821 tccaaataaa cagtgttctc ctgccaaaac ctcggtcata agtcacaata tacataaaac
    11881 atgaaaagct gttttttgaa attatccctt gtgactcaga gttagacggc aggctcacaa
    11941 cctctccact aaacgatact tacattgatc ctttgaacac accgctgttt ttgatgaagt
    12001 aatccacacc tgaatagata gtaaagatca gacaagcata aagcatgatt tgatctactg
    12061 gaataccgat caggctgaat gggatattat tgataaacag caagataata gcaaccattt
    12121 gtgtagccgt cttgactttt cccggccaag ctgcagccat gacttctcca tgttcgacaa
    12181 gtagtaaacg caagcctgtg acggctaatt cacgacagac aatgatcgct actacccaac
    12241 caggggcctt tccttggtca accaacatga taaaagcagt cataactagc attttatctg
    12301 ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc
    12361 catctaacca gtctgtgata ctagctaaag caaaaataac tgcacctact agctgtgtga
    12421 cagccagtgt ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa cctacaacca
    12481 aaatgaaaat cgggatcata caaattctaa gtacagttaa tttatttggt aaattcacac
    12541 tatcgactcc tttcttccct attctacgtg actatcagcg aagtttccac cgatattatt
    12601 tacttttcca aatgaattta ttcaaaggag tatttttatt gaaattgatc tttttgaatt
    12661 aacgaagaag gaccgcgaca cagatttgtc acgatccttc ttttttatac ctacaagtaa
    12721 acagatgcgt aaactaattt atacgatcat tctgcgtatt gaagagtaag gttcacattt
    12781 tttggactct gattgttttc ccctggatta actggtacgg attctccatt cgctttgatc
    12841 gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg
    12901 gtactctgtg tttcatttgc tgccagtgtc ccttgataaa cataggcatt gtctactagc
    12961 acacctaacc atacacgatt gacagcagta aattcaaggg ttacaggctt ggtagcgtct
    13021 gtgatattta ctgcgatggc actgccagaa tcttctcctg tagctattgc cattttcttt
    13081 tcttcacttg attttgttgt tgattctact gtagaagact ctttggtctg cgtgctttgc
    13141 tctgaggaag aggactcctc gactgatcct tctactttaa gcgaagaagc tgtgtctccg
    13201 attattggat cagcttgacg atcttgccaa gtcatatagc ccacgacaac gacaatcgcc
    13261 aaagcaacca atcctaataa aatgacagga agacttgtcc aaaagcgact tggatgcgat
    13321 tcttcctgat gcattgcttt tctagaagta cttactgttt caggctgcgg acgttcaggg
    13381 accgatcctg tcaatttgct tttcccatca aaaacatcga ctaaatggtc tccatcttct
    13441 cctacagctt cagcgtattg ccgaataaat gcccgcacgt aaaatttacc aggcaattgg
    13501 tcaaaatcat cattgtcgat tgcttccaaa taacgttttt gaatttttgt aatctgctgt
    13561 aattcatcta aagatatttt tttattcagg cgtgcttgcc gtaatttttc tccgatggtt
    13621 tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc tccgaagcat ctgctgttgc
    13681 aacgaaagac gttatgtttg ggtgctgtga caaaagtcat gtcacagctc cttttttccg
    13741 aataaacatt gttctcctgc caactcctcg gtcacaagtc acaatatcca ataaatataa
    13801 gaagctgttt tttgaacttg ttccttgtga ctcggagtta aacggcaggc tcacgacctc
    13861 tttcttaaac ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat
    13921 aaggagatat ttttccgtat gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    13981 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
    14041 acattaagaa gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca
    14101 caacctcttg gtacacggtg ttccagaagt agcttcttcg gcaataagcc gaaatatcac
    14161 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
    14221 tcacaacctc tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag
    14281 catgatttgt tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct
    14341 gattttggag gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa
    14401 atactggttt attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct
    14461 tccccagctg ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat
    14521 aaagttcgat cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt
    14581 aatgattgga aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac
    14641 acttccggat catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct
    14701 ggttcgtccg tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca
    14761 aaaccaaagc tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta
    14821 ttgcctaaaa tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt
    14881 ccttcttgcg gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg
    14941 gctgcttcca atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca
    15001 atttcttgct ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat
    15061 ttctcaggct ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc
    15121 cgatagcacg tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca
    15181 atatcgatat gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc
    15241 cagttagggt cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt
    15301 acagtttctt ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt
    15361 tctacctgat ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg
    15421 gcggaagctc cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac
    15481 ttatgttcta aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag
    15541 ggaataaatt cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga
    15601 tagtcatttt taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt
    15661 gtttcattga tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa
    15721 gattgcctgc aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc
    15781 catacgtttc aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa
    15841 agaagcttcg attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat
    15901 tgcttttgtc tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg
    15961 ctcgtgaatc agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact
    16021 catgaatcct cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc
    16081 tctgacattc atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat
    16141 tagcgcaaaa cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt
    16201 tgcttgaacg attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt
    16261 ataaaacaga tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc
    16321 agctgctttg tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat
    16381 catttcttga tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac
    16441 gcccaaccct gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata
    16501 ggaagcaaaa gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt
    16561 ttccaagtca aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac
    16621 ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt
    16681 cacaaaactg atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc
    16741 aaagcttgcg ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccgaataatt
    16801 caaactattt gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg
    16861 cagacgcgca gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac
    16921 atgaagattc acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt
    16981 gattgcccgc ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga
    17041 aatttataag ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag
    17101 catagatgtt ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt
    17161 ttatttttcg atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat
    17221 tcaaagagtt catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta
    17281 gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt
    17341 ggtttgtcgc aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact
    17401 ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat
    17461 ttctgatcac tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt
    17521 tacgctccaa agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta
    17581 gtgacatacg cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc
    17641 aaacaaatct ttccgatact tctaaataat aaagaatcta aaaaaaagaa agccgatttt
    17701 cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat
    17761 atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata
    17821 aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg
    17881 gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg
    17941 atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc
    18001 aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc
    18061 aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat
    18121 cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca
    18181 aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaaag caaacgaacc
    18241 tt
//
LOCUS       DASJUF010000054        18211 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJUF010000054 DASJUF010000000
VERSION     DASJUF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.054"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(116..331)
                     /locus_tag="U4B62_001673"
     CDS             complement(116..331)
                     /locus_tag="U4B62_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141834.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            complement(354..1244)
                     /locus_tag="U4B62_001674"
     CDS             complement(354..1244)
                     /locus_tag="U4B62_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEN2141835.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            complement(1259..1882)
                     /locus_tag="U4B62_001675"
     CDS             complement(1259..1882)
                     /locus_tag="U4B62_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN2141836.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            complement(2009..2326)
                     /locus_tag="U4B62_001676"
     CDS             complement(2009..2326)
                     /locus_tag="U4B62_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141837.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            complement(2328..3380)
                     /locus_tag="U4B62_001677"
     CDS             complement(2328..3380)
                     /locus_tag="U4B62_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN2141838.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            complement(3298..4827)
                     /locus_tag="U4B62_001678"
     CDS             complement(3298..4827)
                     /locus_tag="U4B62_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2141839.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            complement(4839..6254)
                     /locus_tag="U4B62_001679"
     CDS             complement(4839..6254)
                     /locus_tag="U4B62_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2141840.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            complement(6247..7080)
                     /locus_tag="U4B62_001680"
     CDS             complement(6247..7080)
                     /locus_tag="U4B62_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEN2141841.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            complement(7239..7469)
                     /locus_tag="U4B62_001681"
     CDS             complement(7239..7469)
                     /locus_tag="U4B62_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141842.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(8216..8683)
                     /locus_tag="U4B62_001682"
     CDS             complement(8216..8683)
                     /locus_tag="U4B62_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEN2141843.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(8758..9198)
                     /locus_tag="U4B62_001683"
     CDS             complement(8758..9198)
                     /locus_tag="U4B62_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2141844.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(9228..9521)
                     /locus_tag="U4B62_001684"
     CDS             complement(9228..9521)
                     /locus_tag="U4B62_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141845.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            complement(9552..9746)
                     /locus_tag="U4B62_001685"
     CDS             complement(9552..9746)
                     /locus_tag="U4B62_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141846.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(9852..10304)
                     /locus_tag="U4B62_001686"
     CDS             complement(9852..10304)
                     /locus_tag="U4B62_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141847.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(10330..10791)
                     /locus_tag="U4B62_001687"
     CDS             complement(10330..10791)
                     /locus_tag="U4B62_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2141848.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(10992..11843)
                     /locus_tag="U4B62_001688"
     CDS             complement(10992..11843)
                     /locus_tag="U4B62_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2141849.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(11858..12658)
                     /locus_tag="U4B62_001689"
     CDS             complement(11858..12658)
                     /locus_tag="U4B62_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2141850.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(12701..13591)
                     /locus_tag="U4B62_001690"
     CDS             complement(12701..13591)
                     /locus_tag="U4B62_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN2141851.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(13593..14534)
                     /locus_tag="U4B62_001691"
     CDS             complement(13593..14534)
                     /locus_tag="U4B62_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN2141852.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(14767..15102)
                     /locus_tag="U4B62_001692"
     CDS             complement(14767..15102)
                     /locus_tag="U4B62_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141853.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(15258..15431)
                     /locus_tag="U4B62_001693"
     CDS             complement(15258..15431)
                     /locus_tag="U4B62_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141854.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            complement(15428..15616)
                     /locus_tag="U4B62_001694"
     CDS             complement(15428..15616)
                     /locus_tag="U4B62_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141855.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            15694..15918
                     /locus_tag="U4B62_001695"
     CDS             15694..15918
                     /locus_tag="U4B62_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2141856.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(15915..16070)
                     /locus_tag="U4B62_001696"
     CDS             complement(15915..16070)
                     /locus_tag="U4B62_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141857.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(16210..16422)
                     /locus_tag="U4B62_001697"
     CDS             complement(16210..16422)
                     /locus_tag="U4B62_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141858.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(16425..16637)
                     /locus_tag="U4B62_001698"
     CDS             complement(16425..16637)
                     /locus_tag="U4B62_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2141859.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            16912..17322
                     /locus_tag="U4B62_001699"
     CDS             16912..17322
                     /locus_tag="U4B62_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2141860.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            17335..17787
                     /locus_tag="U4B62_001700"
     CDS             17335..17787
                     /locus_tag="U4B62_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2141861.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            17878..>18211
                     /locus_tag="U4B62_001701"
     CDS             17878..>18211
                     /locus_tag="U4B62_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN2141862.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEI"
BASE COUNT         5214 a         3709 c         2752 g         6536 t
ORIGIN      
        1 tctagcaaaa ttatttctaa taaattagtt atgcgttctt cactcaaatc tggaaaatct
       61 tcttttgtag actcaatgat ttccgttttt agcgattcat ccatcagatc acttcctaaa
      121 tttcattaac atcaattgca tcttccaaaa ttgcaattgc ttctttatca tctatagaaa
      181 tgataaattc gttatttgaa tctgctgtga taaatctacg agttttagga tgaacaaacc
      241 caacaaaatt tttagtttta cctactctat atttcacgac ttcatctttt ttagtttcat
      301 ctttttttac cactgtaatt cctcctatca aaaaagggag tactaactcc cttttattca
      361 gactttaaat ttaaaattgc cccagagttt gaagcattgt attcaagaga gtattcacct
      421 acaagaccga tacgtctaga atccgttgtt tttgcaagtt cttctgctct ccattcacgt
      481 aatggacgta gtttcacata attagtatcg attgcggcaa ttgttccttt tggcaatgat
      541 ggttctagta gagcaatacc tgtaccgtaa ttagacacga tatttccaat ttgcaatcca
      601 aaagttactc tttccccgaa ctgaacaatt tttgttgatt ttccatctaa ctcatcagtc
      661 attaactcct gcatatctgg agaaatcaag catagttttt ctcccatata acctttttca
      721 tacatcaatt taaataaagt atcgatatct tttcttgtta ctgcacccgc agtggctgtt
      781 tctgccttat ttgctgaatt gatcaagttc aaaatcccat tcattcgacg acctttagaa
      841 ccattttcat cagcttttac accagtaatc aattttcggt tcaaatcaat tttcatttcc
      901 ataccacgta gtgctacttg attagttaat tcatttccga ctccattcac attaatagca
      961 tctaatgtac cagatacaga ggttgatttt ctaaaaattt cagtatagtt gttaaaccaa
     1021 gttcgacctg attcagcatc tggatattct ccgccctcaa gtttttcaga agaatcatcg
     1081 ttgttgatat catattcacg ccatttaatt tctgtcgagt ttgctggctc tgtttttcca
     1141 gcaccaagta aatagcttaa aaaaggtgta tttggtactt gcatagcatt aatcgctggt
     1201 gaaatatcca aatactctaa attatttaaa gatgtttttt tcataatttt tcactctcct
     1261 agttaaattg ttgaagaatt tgtcctaatt gttcttctgg atttgttaat gtttcattgt
     1321 tctgctgaac ctggttattc gattgttgag ttccaccaaa tgcagacttc atttgtagtt
     1381 cttttaatgc atctgcatgt ttctcattaa ttgcattcaa aacacttgta aaaccttcaa
     1441 cagcattctt agtgaaatct gtatccgaac taacaagatt atttagcata aattgagaaa
     1501 tagactcttt taactctcca tctagttcta atccagcaat ttgttctgca acaaatgctt
     1561 tattatcact tgtcacacgc aacgcttttt ctgcttcaaa ttcagcttgt agtttttcca
     1621 gttgaatttg ctcaggtgac ttgtttttct tagattcctc atattccttg atggtatcct
     1681 gtttaatttt atctaagtta ttttgtttcc aagcttccaa ttgtttgtcg gcaacacttt
     1741 gagattgtga ctgaataaat ttttgagctt cttcattgga ttccacaaaa gatttgaagt
     1801 catcaaaaga aaacttttga tcgtcacctt cggcaaagta ttgcaaattc attggcatta
     1861 atgatttgtg tttcatgctc attctccttt cgccccacga ttcgttttca cgccccgcat
     1921 tgctttgagt tttaatagtt gcgccccacc attcaaccaa gcccagtatt gcgctagttt
     1981 aacgtcattt cggacaaaat aaaaaagcct aattactaga ctttccttct ttgtagtcag
     2041 ttctaacaac aatacatccc atcttttcat accaatcaac tgtttctttt aaatttggta
     2101 atgaatgaga taataattga atcgttaaat taacggtatt cttatcaact ggagtattat
     2161 cttgataatt aacagtttga tcgccaataa ccacgtaggc ataattgcca ttccaatggt
     2221 ctctcaagcc attaggatgg ttctcagact caatagctgt tttgtaggct tctgcaatat
     2281 cagcatttac attaattgtt agtactgctt caaaaaaatc tttcattcta cttcctccgt
     2341 ttcaaattaa ttccaaaaga atcatgtgca aactcatcta ataaatcgcc aaataaacgg
     2401 tcatattctt catcaatatc ctttcctaat tttggtaaat taggaacatc cgtacatcta
     2461 caacgactat ggaaaggtgc tcgattttca cctatgacag catctttcag tttataggga
     2521 ttcttacttg cctttcctcc acaaatccgg caaactctct catcttttgc agtcaatacg
     2581 ttgtattcct caatacctgt ttctaaatat gatttttcaa taccatcttg tgcaaatttt
     2641 gcatactccg ttcgaacgag attttctatc gctttgttat actttgattc ttctaactta
     2701 aacatgtcac aaatttctga gtctaatctc atggtgttta atgcgtgtat aacgccttct
     2761 ccactagcaa cactttttac aatagcatta gagagtttct gttcaagcga tgaaatattt
     2821 ccccaaagtc ttcttgaaaa tgtctttcct gaccatggat agttcaaata atgtttaatt
     2881 tcattttctg gtaaataatt tggtatatca acgtttaaaa gttgaacaaa aacattagca
     2941 ttagaagaat atgttctttg aagaatttcc tctaagcgat cagaaaaata ttgattcaca
     3001 tctgcatcca tcgcatattc tgcaagaatt ttaaatacat ctgatcgtat ttgtaataag
     3061 cgattgactt ttgcatagtc aaatggtgga aaaaattcat cgataaactc tttgtatttt
     3121 gcatcataca tttttaatga cttataattt ttttccacat attcacgata ttttttctga
     3181 ttacttgtac tgtaaaactc catcatttct ggataagtga tattgtgtaa gtcagcttgt
     3241 gataatagtt ttgcttgtat ctctttcagt gcttcaggaa atacactagt taatttttta
     3301 agtgtctgat tttcttgttt gagccttgct tgatcctcca gttctcgacg tttggtccaa
     3361 tacttcgttt ctatcgtcac tattgccacc tccgccaaaa ttgtattcac tatctggata
     3421 agcacttcca ctttccaatt ccatcatttc attttcataa tcaacatctg ttacaaatgg
     3481 aatttggctc tgaatagttc gtttggatac gtatggtgca agtttaggta aagcttcagc
     3541 taaataactt aaatcagttg gcaaagatct agagaaagta aaaataattt tttcaggctc
     3601 aatttctact ttgtcatgaa acttaagaaa agcagcaatt gtttctgcgc attctttcaa
     3661 tccttctcta aaatattgct ctttagtatt tgttttcgct tctaggctaa tgatttgcca
     3721 cttgcgagct tctccagaac tgtttgattt aaacacctca tcgttaaaat cgattgcttt
     3781 acaaatggtg taaaactgtt ttttcaatag atccatatga tattcattga aatcttttgc
     3841 taaatttttt gtgacatatt cagcttttgc agtaggggct tttaagttga taatccctag
     3901 ctgattcatc atttctttag cagtgccttt gctcattgtt gtgccagtaa caagcatgta
     3961 cgcaagctta aattgttcga tttcgttttg ttgatcagac aaagcacgat caattgcatc
     4021 tccaatttcc tcagctactt caaaatcaca ataacgattc gtattatttt taaattctgc
     4081 caaggtaatc gtccccaaag ggttatcggt ctcatcaatt tttttgaacg ttccgcttgt
     4141 aacaaaattc aaatcaccat agcaagcata tgtcagaatt ttatttcttg tcataacttt
     4201 catttcttga aaaaattttt tctggtaaga gtcatacttt tctttgatat agattccggc
     4261 atttccatat ctttctgctc tccaaggttc aatattactt gctcttaact tccaaccatc
     4321 atcttcttcc acaggctcta gcaatcggaa agctactcca caagctcctt gaaaagttgc
     4381 agtttcagaa tcaagcattg cgaatctcat ggaatcaaga ttgcttgtta atttgttaaa
     4441 agcttctgga actgtcggaa gttctatatc attttttaag aatttatctt ttaatctttg
     4501 aatgagtgtt cttttttgtt ccgatacatc gtaatcccat ttgataggta taccagtaaa
     4561 atgattgact gcttggtcta cgacaattga atacatacca gcatgaagtt tattattaac
     4621 ttttacaata tcagtgttag gctttggtct attatctatt tcattctttt cgctagtata
     4681 agctagatat tttctttcgc gatcagcaaa aaatggtttc atatcttcta taaaaccatt
     4741 cggatcaaac attccatctt caatttgtgt tgcatattta acacgtagct tcttatatct
     4801 ctctaaagat aatactgtac taatcaaaag ctcacctcct aaaattgaat aaattgatag
     4861 tgacttggtg gctcataaaa cgctaaagcc agtgcgtcag caatatcggg actaccaata
     4921 tttcgctttt tcatatcatc tttactttct aaacgtatac gacttctact tgtcattttg
     4981 aatttgcgag tacttaattc tttgattaac gaactatcag aaggcaattc aataacaggc
     5041 tgttctccgt taagatttgc tgtcatattt tcttctaaca tttccttgat gtttccccat
     5101 agttgagtac ctaaattatc gtaaaaatcg tcttctgatg ttgaaccgtt attcactcca
     5161 aacacctcaa aaggataatg tttgtcttct ataagttctt ctaggcggtc ggttacacca
     5221 cctccgacac cagtgtcatc gactttaatc atcactttat caatactcgg atattgactc
     5281 attagattct tggccatgtt gatgacatat cctgttgttt ccatggtgct acgttttgaa
     5341 tacttctcat actccaatgc cctggtagca attctaggaa agagaatcgt agaatcatca
     5401 ccatatcgag ctacgtcgac accaatatga gcaacctttg ttttattgac taaagaatca
     5461 ctaatttgtt tttctgtagc caattcaact gtttcaagac tgataaatga atccaacgca
     5521 cctttcggaa attctccaaa aatacggaca cgagcaacat cactttcttt tccatatttt
     5581 ttaagaatca tttctatatt gtctttgttt gtacgtttac tatcatagct tgatacttta
     5641 tgaactctgt atttatcacg gtctgaatta tgggaatcat aaaagacacc ttcaatatta
     5701 ttggggtttc cacacatcaa cagcttatta tcaaaacctg atagcgtacc aagaatagct
     5761 tccataattg gatcagacac accagaagct tcatctacca caatcaacat atggtcctcg
     5821 tgaaaacctt gcatactttc tggtttagtc gctgttctag ccgtagcaaa ccaacgctct
     5881 gaatcaccaa ccatataaat tttggtcttt gtccacttca gtaagttttt gatcaagcta
     5941 tcatttaacc atttagctac ctctgcccaa agtacatcgt aaagttgctt cattgtcgga
     6001 gctgttgcta ttactttagc gtagggtcga cacgttaaga accaaagtat tgctcctgct
     6061 tctaatgctg tttttccaac tccttgacca gaacgaacag aaacctttga aaattcagct
     6121 aaatcattta aaacattttc ttgccattca tcaggattca aatgcaaaat atcttggcaa
     6181 aaagctactg gtttatcata gtaataatca atggcagaac caatatcagc aaaaggaata
     6241 aattcattat tcattttctt tcaccgcccg tttgtttgcg gcatttaaaa ctgcctgttt
     6301 ccattcttct atttcttcac ctgaattatt tccgccaatt tcatttgtct cagcttggat
     6361 cttatcagtc tgagctttaa ttaaatcagt tctgtattca ttcatataaa gttcattcat
     6421 ttgcttgatg gctttagcta actggttgct aatccgagtc aatgaatctt caattgaaag
     6481 aatatcatca attttgcgat gtttttttct gctaatctgc acatcttgca taacctcacg
     6541 cttgatttct agctttttac catttttttc aatcggagtt ttaatctttc ttagctgctg
     6601 caatcgttcg acttcttcat catttagtcc agcttcagct tgttggattc ttttcatcat
     6661 tcgaagttgt cttattttaa gaagtcgtat ttcttcggac aaagcaaaag aaggatcatc
     6721 attcaaacta gaatagatgt ccttctcatt atcggttaac gtgtcaaaaa atattgtttc
     6781 atactcacct gttttcaaag cgtttttatt acccactggc ggagaagctc ggctatttcc
     6841 tttgttacct ttagcatttt gattaccaat aggagcgcca ccttgattag taacgttact
     6901 tttgccattg gtaacgttac taatcaattc agcgctccat ttgtcttgcg atttccattt
     6961 tctaacctga gaatctgaaa catttaattc agatgcaatt tcctttaact gcttttctcc
     7021 gttggattct aaccaaattc ttttggcttc atcacgtctt ggatcacgtt gtcttgccat
     7081 tcaatacaca ccacctcaca ttctgtttag gttgagtttt gttttctaat tttcaatatc
     7141 ttttagcatt aaatcagctt caatcaatat ctttaaatcg gaaactttgt ctaacttgat
     7201 ttgtcctgat tggagatttt taagccattt cttgcattct aatccctcaa attcttgtta
     7261 atattatttt gaatgtttgg ttcatcatag aaaccatgac cgcaataaac gagcttgcac
     7321 gcatcaatct cacatggttc tgcttctctc cccatttcga caatagagta cttccctttg
     7381 atttgtacag aacgcacaac acgcactgaa caatcatcaa tggttcgagg atattcatta
     7441 gttagcgata cataccagta gtttctcatt atgtagcctc ctttatgtaa agaaaaagac
     7501 cactcaaaga gtgatctaat atgtaatgca caggcaggga cgtttccgat cctgtgcttg
     7561 agacatttga cgattcattt gtaccgaatc ccaaatctca atctaaccta accagttatg
     7621 taatagcaac ctacacgatg cacaaaacgc gtacgaaatt gcgcacccct atatttttaa
     7681 accgccgatg cctcggttgc caaagtcact ggcaaggaat cgaaccttgc atggttgccg
     7741 aagcattgac ctagcacaca tgcttagcgt ctaccctttc cgccacagtg acactataaa
     7801 attattcttg gctgctacta ttttttattt tgcccatttt taaatccaat catatataca
     7861 ttaagacaaa gcgcaaaaat tgaaattatt aatggaatca tttctcttca cttaccttta
     7921 gttatcgtgt gaataattaa aaaaacaata gacagcaacg gatgatagat aataagaaca
     7981 atttagaagg agttgaaatt cacatcctta ttcttaatat ttccgttgct gcctatcgaa
     8041 gcttaattaa acgatgaggg agatttcctc ccttacattt tattttgtcg atcctgtttc
     8101 ctaatttttc gacactatca taatacaacg ttgaataggt aagtgattgg tataaaaaag
     8161 gtataaaatg gaaaccaaat tggtaataaa agggtataaa aagtgtaaaa actggctact
     8221 tgaaagcaac cagttctaac gatgaagcaa attggatgat aattctgttt gattctactt
     8281 taaccgattc ttcgctagta ttattccttt gagcagttac ataaatgggc agaccattga
     8341 tataacgatc atagaatatc ttcttgcgcc tttcagtcac atcaggctta tgcggatgct
     8401 gtatcgctga atagcctcga acaaacaatt tatgcagata ctcaaattct tcctgtgctt
     8461 cttctttatc gatcagcatt ctttctgctt caaatatatg atcagctgta gaaggtggaa
     8521 ccaaggaata agatgctgtc acttttggtt ctcgatgttg ccctactcta catctagctg
     8581 atagatatgc tgaaagaaac acagcgacat tatgttttgt gcgttccata tcaacatctt
     8641 tcgcgcttgg tgtctcatat ttctttacat cgaaaagtac catcctctga ttcccccaat
     8701 tatgatataa tacttatgtc ggaaatatta ttcatagtcg gaggaatccg acttttttta
     8761 ttttgtacgt gaaatgagtt ctcctgattt atacgcctca gcaaattcaa ccaaagctac
     8821 tgccttcatt ctctcgatag ttctatcgga atatcctaat ttttctccta tttgatacat
     8881 tgacatctta ttaggaaaac aataactata ataaagtacc tggaaatgga ttctacttag
     8941 ccgtgttaat gcttcgataa ccgaatctcg agtacttatc gcttcaatga tttcttcgtt
     9001 ttgactgtca attgtgaatt tcaaattttt ggtaacagtc atcacggaaa agtctatttt
     9061 cactggtccg accaaacgtt ctaaatttct gtatcttttt agtaatcgtc tagcattttt
     9121 ccttgtttgc ttaatatcta tattctctaa aatgttcaca attgcctctt ctccttccag
     9181 ttgtggaata acgctcattg ttgtaggata gttgtaaaaa agcaaaatta agattttata
     9241 acttccatgt ccaccaatct caccactgct aaattctctt tgcttttcgc taaccactta
     9301 tcacattcca tcgtgttttc aatgcgaatg attgctgagt gattatagac gtgttctaca
     9361 tatccacgaa atggatagat gaatccttct gcttcgcagc gaaccatgtc accgactttg
     9421 aattttggtt tcttacgtgt tttaggattc tttgtcggca tatctagcat taaaccgccg
     9481 atgccgtggc cgctggagta aaatccgtct tttagtttca ttccgcttcc tcctctttag
     9541 ctgtcaatta cttactcgca agtcactaaa atggctactt catcgataaa gtttccgtct
     9601 tcgtcttcca ttccttgctg aatgctaatg tcggtaatct gcttaaaaaa ttcgttgtca
     9661 tagccttgtg cgtttatcac tacttcgacg tctccgaaaa cctctttgag actattaatt
     9721 ttatttacta cttccgatgc gtccatgact acaccccttt ccagttaaaa atctctgtcg
     9781 ttaatagact actgttcaaa gtattcatct tcactcatcc ttctgcctcc tctttagttg
     9841 tcattagtgg actaaatact acctgtttga aaaccataat gtgcagtttc aacgccgtca
     9901 taatcactta tttcccactc agccccatcg ggtatatcaa cgactttaag acttgcatat
     9961 ggtccactgg cctcacttcc tagtttttcg acaacagcaa cgaggtcctt atcacctctg
    10021 tttgaatcac ttgaaaaact ggaatcatca gcaattccgt acagtcggtc aatttcgcta
    10081 taaattcctg taactttgtc gattttcggg tcttttttga aatagtagat aaaatccatc
    10141 gaacctagct tttggcctgt gtatctcgtg tattcatcat ctgagtcata gctaaagtta
    10201 ttgatgtatg ggaatacttc aatatttttt cttttaaaaa tttccatttt tgcttcatgg
    10261 ctcaacccaa agccaccaaa acatttattt aatacaatct tcattacttt tcctcctttg
    10321 tttcctccgt tagctgaatt ttttcataaa cacaagccaa tgtgttttgc ttcgtttatt
    10381 accaaacaat ggctgttctc caatagcctc taacacatca gaaagtggta tctgttcctc
    10441 gttccactta aaaattaatg ttcctgaagg tcgtaaaact cgcatacact ctgcaaaacc
    10501 ttgcctgatg tcttctggcc aaagttcgtc taattttcca tattttttgg caagccatga
    10561 gttttcgccg acatgtctta agtgtggcgg gtcaaataca accatatcga acacatcatc
    10621 ttcaaaaggc atatcgcgaa agtctgcgac cacatctgga tcaacattga tcacatgacc
    10681 tgttgataat tcttcatact gctgacgaca atccataaat gttacgtcag aattgttctt
    10741 atcaaaccag aacaatctac taccgcaaca tgcatctaat atttttggca attactctac
    10801 ctccaataac tctggattct cgtagacgtt acctaaaaca gtgagaaatc tttctgtatc
    10861 ccgataaata attagcttac ttatcttccg aaccattcga ctctcttgtt gagataattc
    10921 gtccatgtca cgatcaccta taatttgcaa cattatctga cgacagattc ttttttcttg
    10981 tttagtcaaa ttcatgcgat cgctcctttg actggtttta tgcgcttgtc tgctgtttga
    11041 gaaaatacca tcgtaaatcc atcagagttg acaaacattc ttgatattgt tctcgtaccg
    11101 taagcttttt tcagttcttc accaagcaag ttcgttgtga taatcgttgc taggttctgt
    11161 cgtgcatcca agaacgaatt tagcgtgttt atgccaaacg ctcgactatc tgatgcatct
    11221 tttcctagtt ctgaaccgat atcatcaatg atgactaaat ccgctgtttt gatatctgca
    11281 atcaacgatc cctcaatcgt ttttctgagt tcaggattgt tgtacgaaaa tttgatttga
    11341 tccagcattt cctgtaaccc gataaataag atttttttat cgtagtttga tcgcttcaag
    11401 acttcccaag ccgctgccat cgctaggtga cttttacctg ttccttgttt tccagttaaa
    11461 acaaggtgac ttggatttcc taacaagact gaattaacaa agcgtttggt tacttcaacc
    11521 gcttgtcttg tttcttgatc gacaatttga taattctgca atgtgcaatc aaacaacgct
    11581 tgattaggca caacagaacc acctttgaaa aaattgattg ctcttgcttt caggctctcg
    11641 ttgtaaattc gttctgtttg taagtcttcc tgaactcgca atgctttata cccacattgc
    11701 atacaggttg gcttacaacg ttctgagcca tcagggttct tcgtacgcca tccatacaaa
    11761 ggctgtccgc attcaggaca ttcgccacgt tggacaagca ctttctgtat cagcttttcc
    11821 atgatttccc caacagtttc catgcctacg cctcctctca aataggcaac tcatctgtgc
    11881 taaatttctc gtattcaagc gttttagttt gttttgtttt ataatcacga tttgcttgcg
    11941 ctgccattgt gtcgtatttt tctcgcaatt ttttagcaga taaaatattc gatgcccaaa
    12001 acacattgtg ctgactccat tcaatcattc cccgtacctg gttttcggtc cgcttgtcga
    12061 tctcgatcat tttccgaatg tcatctgccc aactttgcag attcggcttt ttgatttcct
    12121 gattctgaca aatctgtttg aataactcct ccgcgagaat gtagtaaact gagtcggtgt
    12181 cgtaaacacg ctttttgcgt ggttgcgacg atgatgtttt attattcttt tcattcttat
    12241 aattcttttc attcttgttt gtgtgcactt gttgttcact tgttgtgcgt ttgatgttca
    12301 tttgttgttc actgctttgg tacaaagacc agttttttat tgatataacg ctgtatttcg
    12361 tagttgattt gatgttcaac attccgtttt tttcaaattg ttttagccat ctccatacag
    12421 aaccgctgtt cacttgatgt tcacgtttca cgccttcgtt catctcagat gtgatagcat
    12481 cgcgccctgt gacgaattct ccgctgttca cttgtatctc ttttccatta aaaagtatct
    12541 tgcgattctc atgactcgct ttcatcaaac acaagttcca taatttatac atataaggat
    12601 tggtccaaac gaatgaattc atcacttttc gatgtaattt gacatatcct gtgttcattc
    12661 gtatttctcc tctagaaaag caagggagaa agctccctca ttatttgttt aacggcggat
    12721 tagatgcatc aaataatcca gtttgtacat cttcattttc ttcagatata acctctgctt
    12781 cttttctttc aggaatatct tcttcaactt ctgtttcagc aataatgctg ccgtcttctt
    12841 gaaccctttg gactctctca tccgatgtgg tggcttcttg catttcgatg gacaagattc
    12901 cccatttaga aagaagattt ctcaaaacag tttttcgtgc cattgcattg taatcagatg
    12961 cccacacacc acttaacttt gtcttatcgc gatctttatt gttagcaatc cgatgagctt
    13021 cgatttcttg tttggtccaa tagacagttt ttttgaatcc attcagtaac tcgaaatagc
    13081 caacatatcc aatgacttca tcagacgttc taccatttgg atcaaactca aactcttctg
    13141 tcagtcggtt ccagcttttt agttctcctt cgtaaacttc gatcacattt aatgctttgt
    13201 atttacctga tcgttgggct aattggatat atcctttata gccaagcatg aattgagctt
    13261 tcttttccca ttttcctgtt tgcttgtttt tactattgaa tggaactaaa tatgcataac
    13321 ctaaattctt atctagccca agatttaatg ttgcagcagt taacgcacca ctcatgatag
    13381 acatcggttc actatctgca agataactgt cattagatac aagagtcata acattcgaca
    13441 taaaagcatt agcattgtca tgaagcacct cttcgaactt ctttctcatt gttggtgtat
    13501 tcatcaaagc tttaagtcct aattgacctg gtgcaacttg tttctgtggc tttgctgcca
    13561 attgattttt taacgattca tttgttgcca tattatttga tctccctttc ggttagcctt
    13621 cttgattcag taacgttata aatctcttca tcatttgcga catctggata tttctctgct
    13681 agtttcttcg agttcatacg tctcgtacgg acaaatttcc aactgatgat gtttttttga
    13741 gtgataccga tactggcttc acgtttacct agctcgctga taatctcgtt gtctacttga
    13801 cggatagctg attcaatttc tttcttagtc cgtttgagtt cgtttttctg ttcaactagt
    13861 tcatcgaaat gaattggtaa cgtcgtttga acgtcttcaa catctgcata cttctctttc
    13921 aagaaatcag ctgtcgcttg actaccatca ataattggct cgatacctcc aagaacgttt
    13981 gtttcccaaa actctactaa ttgttcagtg attgtatcga tcagctcttg atctcgttca
    14041 atccgcttcc agataaattt ttgaccaccg attaagacag cgatgtaaca atagtctttg
    14101 tttagaacgt tcatgtaatg ttgaacttga cagagatagc taagcggtac ctcttcgcct
    14161 tcccattctt taccgagaaa ttgattggct gttttgcatt ccagaatggc attttctccc
    14221 actacttccc gatcaatatt tgctcttaaa aatggatgta atggatgttc aaatacttgg
    14281 tttcttctgc gtactttttt gcctgtacgt tcctgaaact ctttagcaac cacttcttct
    14341 aaaacattac cccaataagc gggctcactt cctgattctt caagtactac ttgtcctgtt
    14401 ttttctagcc atagttgata aggagatttc catttattca atcctaaaat tgttccaaca
    14461 tccgaacctc cgatgccttt cttacggtct tcaagccatt cttgatggct catttctaag
    14521 gtagatttac tcatcttcat cctcctcatc aattggtgct tcatatggtg gtttagcata
    14581 atcaggatca gttaaatagt tgtcaagatt tgctaactcg ttcatgttta acctcttttc
    14641 tgcttgctaa cacgcaaagc acaagcatgg taattgcgaa taacaagcca aagattacat
    14701 aacttttcgc tacaatcact aacgtgaaaa ataaaataat gagtagatca attgtttttt
    14761 cattcatcat tgaaattccc ccttgagaat acgagtcatc acatcagcta aatcttcctt
    14821 gttattaata acaaaagtat gatttgaagg ttgattttct tcttcatttt tcgcttgttc
    14881 gtaagcatcc acagcaattc tacgcataat atgtgctgac aatgggagat tttcatctaa
    14941 atgttcttct tgcatagcaa gacaaagtaa aatttctgtt gtctcaccat aaacgagact
    15001 tttaagttcc ccttcttttt gggctgtaca aataatagaa acaccttgct tttggcattc
    15061 acgtttcaat tcttcgataa gattttcaat tttcttattc atgtgatata attctcctag
    15121 ttttataaat ttgtatgtga ctctttgctg gctggcagag tcactttttt atttgttgcc
    15181 atgctttttg cttttcgata tgttgcttgc ttaaaataat agggcggcta tttccccacc
    15241 gattatcagc aatcacttta ccgattttta gcgcttcttc tcgtgccata gttgctcctt
    15301 tcttttgaat caagcagatt gattaaaacc atcaatgctg cgaacaagct tcccccgata
    15361 atactttggt gcgctacaat cactaataac cccaaaatga atcctataaa aagtgtgtct
    15421 gtcttcttca taatctaatc tccctatttt ttatttctag cattctcaaa tcctcaagtt
    15481 cagaagcaat tagttcagct tgcctatctg atagctcatc ggcttttcta agcgctgcac
    15541 ggtcatcttg taattgtttc ctgcgttgtt taatcaaacc gagaatttga tgttcttgtt
    15601 gcaaggtata tgccatatca cttctccttt cgtttattga atttctccat ttctatttga
    15661 taaaatattt ctatagaaat ggaggtgaaa actatgggaa aaaatcaaca tgttgtacct
    15721 aattccaatg gtggttggaa tgtcaaaggt gctggaaatt ctaaagcgac tgcacatact
    15781 aatacaaaat cagaagcagt aaaaatcgct agagagattt ctaaaaatca aggatctgag
    15841 ctatttattc atggcagaga tggaaaaatt caaagccgtg atagtcacgg aaacgatcca
    15901 cacccaccaa aaggttaatc atatttagga gttaatcgaa tacgataacc gtcagcagga
    15961 gttgcatcgg tcaatgtaac ttctgctact tttttccctt cttctgtttc aataattaat
    16021 cttgtgtaat tgttctcatt caaaatccct atttttgatt cttccttcat gtcatccctc
    16081 cttcgtctct cgccgtcacc acctccaaga agaggagttg ccaaaatgaa aaaacgtgga
    16141 ctttggcaaa cttgaaagga gagcagcaac tcctcttttc agaagtggtg aatttagatt
    16201 ttttagctat taacaaacag ctctaactct aaatcgattt ttagaacgcc atgttctaaa
    16261 ctgttcatat aaatcgacat caattgttat ttctttggaa gtaactgcgt tatacccctc
    16321 actaaaacgt gattttttaa attccttcat tcttctttga taagtacttt cagaatactt
    16381 atatttctct ttaaactccg ctttgctcaa acgttgtacc atttttacgc ctcctgttct
    16441 gatggctgat cattagaatc tacttctaac ttgatatcca aaagttcttc gatttgtttt
    16501 aaacgttcct taggttttct tttttcgatg aagatatctc gtaaataagt aattgatata
    16561 cccattcgtc gagctaactc tctttgactc caacctttct ttactaaagc aattaatact
    16621 tgctgtttta aagtcatgct tcataccccc ttatcgaata ttttctaata tttttgtcta
    16681 ataagtttga caaaaactgc aaatatgcta ttatgtaagc atacaaaata cagcgcacaa
    16741 aaagcgagct tactgatgtc tggggagact attagtttat tgcttttagt ctttgtatgc
    16801 cgtaaatatc ttgcttacaa aactagtata actgcaaatt ctctatttgt aaatagataa
    16861 tttgcagaaa aatacttttt ttgtcggtat tttgaaagga atgcatatcc tatgctatta
    16921 gaccgtataa aaatcttatt aaaagaacgt tcaatgacgt tggcagaact tgaaagaaag
    16981 ttaaatttcg gaaatggaag catgtctaga tggaataaaa gtactccatc tggagataaa
    17041 atacaaaaag ttgcagatta ctttaatgta tcaactgatt atcttttagg acgtactgat
    17101 aaacgccatt attatgattt aacagaaaaa gataaacaag atattgcttt acaagctgaa
    17161 aaattacttg aaggcttgaa ttcagatgct gaaacaaatt attatggtga acctttagac
    17221 gatgaagata aggaaaaatt atacgatgcc attcaacttg cacttgcctt aaccaaagtt
    17281 aaagcaaaga aaaaattcac tcctaaaaaa tatcgtcaat aggtgggctt tggtatgcat
    17341 ttaaacaaat actggatatt agaagaaata gaaaaattat ttgtagagtt tggatctgat
    17401 gcgattaatc catataaaat aatcaaatat tctgatatag atttaattga aactgattta
    17461 gggaataaaa cattaggaca aacgatacac aataaaagat gttatgtgat attaattaat
    17521 caaaacctat caccttctat gaaaagattt gtattatggc atgaaatagc ccacgttaga
    17581 ctccataaag gtatttgcac agcagctttt agagcaaaca accttagttg tatgatttat
    17641 ggtatagaag ctgaagctaa tatgtttgca attgaaatgt taactagaac aatagatgaa
    17701 caagatttat ctgatatgag tcagttccaa attattgaat atttagggct tcctcataat
    17761 cttgaacatt tagtattaca acattaaaaa taaaaaagcc catgctttca catggactct
    17821 ttcctcattt ctgagtaaac tctacaataa ttatatcaaa gaaatgagga gttttgaatg
    17881 ttattattga ttggacttat tttattaatt ataggaatca ctaaactgtc gaaagctaac
    17941 acaataaaaa aagaagctta tgaattagaa caaaaaaatg atgaaaaact aaataaatta
    18001 catgatagag aaagatttat attaagtaag gaagaaacac tatcagattt aaataaacaa
    18061 attactatgt taaaaaatga tattatttta ttaaaaaaag agaaagaaga tttaaatgat
    18121 gaaataaatc aaaagtatga tgaaacatta aaaaatgtct attcttcact tatcgtttca
    18181 aatatagaag aaacttcatc ttctgaaata a
//
LOCUS       DASJUF010000055        16512 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJUF010000055 DASJUF010000000
VERSION     DASJUF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.055"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(354..716)
                     /locus_tag="U4B62_001702"
     CDS             complement(354..716)
                     /locus_tag="U4B62_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2141863.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            939..1691
                     /locus_tag="U4B62_001703"
     CDS             939..1691
                     /locus_tag="U4B62_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2141864.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1831..2754)
                     /locus_tag="U4B62_001704"
     CDS             complement(1831..2754)
                     /locus_tag="U4B62_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2141865.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2769..3041)
                     /locus_tag="U4B62_001705"
     CDS             complement(2769..3041)
                     /locus_tag="U4B62_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141866.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(3511..5136)
                     /gene="groL"
                     /locus_tag="U4B62_001706"
     CDS             complement(3511..5136)
                     /gene="groL"
                     /locus_tag="U4B62_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN2141867.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(5187..5471)
                     /gene="groES"
                     /locus_tag="U4B62_001707"
     CDS             complement(5187..5471)
                     /gene="groES"
                     /locus_tag="U4B62_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN2141868.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            5696..6358
                     /locus_tag="U4B62_001708"
     CDS             5696..6358
                     /locus_tag="U4B62_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2141869.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(6434..7726)
                     /locus_tag="U4B62_001709"
     CDS             complement(6434..7726)
                     /locus_tag="U4B62_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2141870.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            7896..8525
                     /locus_tag="U4B62_001710"
     CDS             7896..8525
                     /locus_tag="U4B62_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN2141871.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(8628..9437)
                     /locus_tag="U4B62_001711"
     CDS             complement(8628..9437)
                     /locus_tag="U4B62_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2141872.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9492..10361)
                     /locus_tag="U4B62_001712"
     CDS             complement(9492..10361)
                     /locus_tag="U4B62_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN2141873.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(10362..11678)
                     /locus_tag="U4B62_001713"
     CDS             complement(10362..11678)
                     /locus_tag="U4B62_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141874.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(11675..13510)
                     /gene="walK"
                     /locus_tag="U4B62_001714"
     CDS             complement(11675..13510)
                     /gene="walK"
                     /locus_tag="U4B62_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN2141875.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(13515..14219)
                     /locus_tag="U4B62_001715"
     CDS             complement(13515..14219)
                     /locus_tag="U4B62_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2141876.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(14409..15269)
                     /locus_tag="U4B62_001716"
     CDS             complement(14409..15269)
                     /locus_tag="U4B62_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2141877.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(15256..15825)
                     /locus_tag="U4B62_001717"
     CDS             complement(15256..15825)
                     /locus_tag="U4B62_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN2141878.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            16206..16278
                     /locus_tag="U4B62_001718"
     tRNA            16206..16278
                     /locus_tag="U4B62_001718"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16239..16241,aa:Lys,seq:ctt)
BASE COUNT         4789 a         3461 c         2810 g         5452 t
ORIGIN      
        1 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
       61 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
      121 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
      181 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
      241 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
      301 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
      361 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
      421 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
      481 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
      541 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
      601 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
      661 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
      721 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
      781 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
      841 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
      901 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
      961 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
     1021 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
     1081 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
     1141 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
     1201 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
     1261 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
     1321 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
     1381 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
     1441 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
     1501 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
     1561 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
     1621 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
     1681 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
     1741 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
     1801 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
     1861 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
     1921 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
     1981 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
     2041 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
     2101 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
     2161 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
     2221 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
     2281 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
     2341 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
     2401 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
     2461 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
     2521 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
     2581 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
     2641 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
     2701 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
     2761 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
     2821 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
     2881 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
     2941 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
     3001 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
     3061 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
     3121 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
     3181 atactgtaaa tatatcttta ttattagtga atggatattt acttttaata gataaaatat
     3241 tataactatt aaaaataact attttcatta taattacaca caaaaaagag cgtgagacaa
     3301 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacagcggaa gcagaagcaa
     3361 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaatttccg tgaatttctt
     3421 cttatttctt ggagttaaac gcttctgttc cagcctcttt ttatctgtct ttcactaatg
     3481 atcagttgta gataagcaag ttgctttttc ttacatcata ccgcccatca ttgatggatc
     3541 cattgcagga gctgctggcg ctgctggttc tggtttatct gcaacgactg cttctgtcgt
     3601 taataacaag gcagaaacgg aagcggcatt ttgcaaggca gaacgtgtta ctttagttgg
     3661 gtctacgata cctgcttcaa ccatgtttac ccattctcca gtagcagcat tgaaaccagt
     3721 gcctagttcc acgttcttca atttatcaac aatcactgat ccttcataac cagcgttttc
     3781 agcgatttga cgaattggtt cttctaaagc acgaacaacg atcttgatac ctgttgccac
     3841 gtctccttct gcttctacag cagaaacttt gctgataacg ttcactagag ctgtaccacc
     3901 acctgagacc attccttctt ctactgcggc acgtgtagca ttcaatgcat cttcaatgcg
     3961 taattttaat tctttcaatt ctgtttcagt agctgcacca actttaacaa cagctacacc
     4021 acctgctaat ttagctaagc gttcttgtaa tttttcgcgg tcaaaatcag aagttgtttc
     4081 agcaatttgg tttttgatca attgcacacg agcttcgatt gcttcttttt ctccagaacc
     4141 ttctacgatc gttgtattgt ctttatctac aacaacttta gaagcatttc ctaagttttc
     4201 gattgttgca tctttcaatt ctaaacctag atcgtcagta attactgtac cacctgtcaa
     4261 aatagcgata tcttcaagca ttgctttacg acgatcgccg aaaccaggag cttttacagc
     4321 tactacgttg aatgttccac ggattttgtt caatacaaga gttggcaatg cttcaccatc
     4381 tacatcgtca gcaataatca acaatggacg tgattgttgc aagatttgtt ctaacaatgg
     4441 cagaatatct tggatgttag agattttttt gtctgtgatc aagatatatg ggttttctag
     4501 aacagcttcc attttgtcgt tatctgttac catatattgt gataagtaac cacgatcaaa
     4561 ctgcatacct tctacaacat ctagttctgt ttcgatccct tttgattctt cgattgtgat
     4621 gacaccgtcg tttccaactt tttccattgc atcagcaatc aaatgtccaa ctttatcaga
     4681 acccgaagaa acagctgcta cttgtgcaat ggcttctttt gaatcaacaa cagttgagat
     4741 attatgcaat tcttcgactg ctgcttttgt tgccaattcg atcccgcgac gaatgcctag
     4801 aggattagca cctgcagtta cgttttttaa accttcgcga acgattgctt gtgtcagaac
     4861 agttgctgtc gttgtaccgt ctcctgcaat atcatttgtt tttgatgcta cttctgatac
     4921 aagttttgca cccatgtttt caaaatgatc ttctaattcg atttcttttg cgatcgtcac
     4981 accatcattt gtgatcaatg gagagccgta agatttttct agtacgacat ttcggccttt
     5041 aggtcctaat gttactttca ctgtatctgc taatttatct accccgcgta acatagctgc
     5101 gcgtgcgtct tctgcaaatt tcaattcttt tgccatgatt gattcacctc attgattatt
     5161 tttgattatt tttttatctt ccaaatttac tcaacaatcg ccatgatatc ttttcctgcg
     5221 acgatcagat attcttttcc ttcgtatttt acttctgtac cggcgtattt ttcgaacata
     5281 acttggtcgc ctgcttttac agcggcagga actttttcac cgttttctaa caaacggcct
     5341 tcaccgactg caacaactgt cccagtttgg ggtttctctt tagcagcaga agctaaaacg
     5401 attcctccga cagttttttc ttcttctttt gcgacttcga taatgacgcg atcacctaat
     5461 ggttttaaca cgataaatcc ctccgataac tcttttttta atctgttagc actcttaacc
     5521 ttcgagtgct aacctacatt tttaataata ctcattctcc ctcttttttg caagctttta
     5581 aatcaaagaa aagaaatttt attctgacag ctgcttattg aaattatgat ataattgaaa
     5641 tcaatcgata taagcttgcc ttgcatgctt gttatccctt aaaaggagga attacatgtc
     5701 tttaaaaaaa tatagcttcc tcagcatttt ttgctatggt ctaattttta tctcaccctt
     5761 gatcctagct tcgataggac ttgtaaaatc tacgtctgaa ttgatcacag caactgcagt
     5821 catgtatata ctaggcgccg ttgtcctagc tattttttat tttaagcaac gtgaaccctt
     5881 agcgatcgaa tcagcggtaa aaaaagcttc tgctccaaag attgttattt atggattggt
     5941 tggaatcttt gtcgcattga ttctgcaaag catcgctgtc atgttagaat ctgtattgtt
     6001 tggcaaagca accccttccg agaatacaca aaatatcatc cagatgatca tggaagcacc
     6061 cgcttttatt ttggcgacaa ctgttgctgg acccatcatg gaagagttcg tattccgccg
     6121 cagtatactg gggatcatca gtcgatattc aaatttttgg gtcggagcag tgatcagctc
     6181 tttgctgttt gcgtttgctc ataatgatgg tcatctgcta atctactttt tcctaggttt
     6241 cttcttcagc ctagagtaca aagcaactgg tcggatctgg acatcgatga tcacacacgt
     6301 aggaatgaac acattagttg ttcttgttca attagctgtc caaaaagggc tagtataaac
     6361 atgactataa atactcctgt catcctagat tggtgatttt cccaatctaa agcgacagga
     6421 gtatttttgt tttttagtcg ttcatctttt ctgctgctcc atcatttttt gtctgtcttt
     6481 cctttttgca gtttgcagaa tctatttttt tcaaaaatgg gaaataaatg aaaaaactaa
     6541 ttgacaacac gataccttgt aaaatcgctg caggcactcc gcctactaag aaacccgaga
     6601 ggatcggcgg tgttgtccaa gggacttgga ctgctgtaaa taaaggaacg aatccaaaat
     6661 ataaagcgga ataggtaatc aatcctgaaa cgaccggtgc gaaaataaaa ggaacagcca
     6721 tcatcgggtt catcacgatc ggtgtggaaa aaataattga ttcattgatg ttgaaaaagg
     6781 ctggtaaaag agcaagattt cctaattctc tgtatttctt cgatttcgca aaaaccgtca
     6841 taaaaaatac agcccctaac gtcaatccag cacccgtaac cgtcatgaac tgatcgagaa
     6901 attgctgagt aacgatatgt ccgccatttg ccaccgtcaa ctcttgtcct gaggctaaga
     6961 tttctgcatt tgctaatgaa ttcgcttgca agacgggact aaccaaccca ttgaccatcg
     7021 cagatccatg aagaccaaat aaccataaaa acggaacaaa aaaacctaat aaaagcacac
     7081 caaacaacgt gtctatgaac gattgaatgg gaagctggac atatgtccat actccttgaa
     7141 caacagtcat tctgaacaaa tgttgaaaaa ccgctgaacc gataagcgta aaagtgacta
     7201 taacaatgat tggaatcaat gaagtaaaag actgccaaac attttcaggt atcttctctc
     7261 tttgcttcca ccctttcgca agtcttacta aacgtgcata gccccatccg atgatgagcc
     7321 cagttgtaac tgctcctagt atcccttcag cacctgtcca gacagtatct atctgagtat
     7381 ttagtggctg aataagcagt agataagcag acaaggctgt tatgccgact ggtaagcgat
     7441 cgatgtcttc ttcttttgca taagcgtatg caatggcaaa aacgatccaa aaggctatca
     7501 catcaaatgt agcctggtat acttgtgtaa aatacataga aaatcctgtt ttgtctatcc
     7561 actgagcgat gtaatccaca ggaaaatgag caaataagaa aaacagcgag ccaacaataa
     7621 ttattgggat cgtgtaagtc attccattac gcaaagcagt taagggtcta agattcacaa
     7681 cagatgttat cattggtaaa aaggcacgat ctaaccactc tttcatttct tatctccttc
     7741 aacttctatt tttatcaatc agttttctta atttaactat acccaatgat cacgaaccat
     7801 gaaaggaatg cgcactgagc ttttcaaaaa tcggattctt tgacgaatat tcagcaactg
     7861 cttacaatgg acaggaataa aaaaggaggt tttttatgaa aattgcactc attggagcta
     7921 ctggccatgc aggttcattt attttagacg aagcattgtc cagagattta gacgtgacgg
     7981 caatcgtgcg tcatccagaa aaattgccat cagatgttcc gtttatacaa aaggatttat
     8041 ttgagttgac aactactgat cttgcatctt ttgatgttgt cgttgatgct ttcaacgctc
     8101 ccaaaggaaa agaagaaatg catcaaactt ctttgtccca tttgattgag atgcttgaag
     8161 gaaccgatac acgcttaatt gtcgttgggg gtgcctcttc gttattctta gatacagaaa
     8221 agacgactcg tatgattgac caagtaccta aaaatgcacc cttctatcca acagctttca
     8281 atatgtacga aggattgtta aaattgaaac aatctaaaaa tctacattgg acttatatca
     8341 gtccagcttc tttatttgat ccacatggca aacgtacagg tacttatttg ttaagtgatg
     8401 attatttaca aagaaacgct gctagcgaca gtacgatcag catggcagat tacgcgattg
     8461 cattagtaga tgagatactg gatgagaaac atgaaaagca gcacatcagt gtagtcagtc
     8521 gataatacgg ttattttttt aatatgctaa aaaaataaaa atagaagata aaaccaaccc
     8581 tgattgattt tatcttctgc tttttatatt tttattgatt gaactgttta aatcgaaaat
     8641 agctcagttg cttcatctgg atctgtatca tagatttcaa aatcatgtcc aacgccaaaa
     8701 tctttttctt tcaaaacatt ttccattgtt aaatgagcaa gttctttcat attgttttcc
     8761 ttgctaagat ggccaagata gattcgtttg gtccgattac cgatcacatc agtcatgaca
     8821 agtgcaccgt cttcgttgga aagatgtcct ctatctccta aaatacgctg ttttaaattc
     8881 cacggataag gtcccatgcg taacatctca agatcatgat tactttccat caaataacca
     8941 tcggcatttt caatgacacc tcgcatataa tcactgcaat atccggtgtc cgtcaacatg
     9001 acgaatgacc gattttcttt atggaaacga taaaattgcg gagccgctgc gtcatgggag
     9061 acgccgaaac tctcaatatc gatatcgccg aatgttctga ctttccccat ctcaaataga
     9121 tgtttttgtt ccactggtac atttccaatc agtggttcca tcgcttccca agtctgctga
     9181 ttggcaaaaa catccaaatg atacttacga gccaagacac ccacgccatg tatatgatct
     9241 cgatgttcat gcgtcacaag tattccatct aattggtccg gtgtccgatc aatttgagcc
     9301 atcaaggaag tgattttttt accgctaagt cctgcatcga ccaaaagccg ttttttttca
     9361 ctttcgatat aaagtgaatt acccgaactt ccacttgcta ggatactgat tttaaaagct
     9421 ggtttttctt cagccatttg actcctactt cccttcttac tttgtaaact tttttcatta
     9481 tactcgcttt tttattgatc ttccactttt ggtacagtat tgtttgtcac gatcgtattg
     9541 ctgaaagcgt tgacgctctc aatttggacc actttatcgc ttgtttcgat cgctacatgc
     9601 caaacaggaa catacacatt tttttcccgg actttcagaa tacaggaata agctagctga
     9661 cgccataaaa tttttgatcc tcttggtatt ttgttattga tgtacaatgt attgattgct
     9721 tcctcttccg tatacagttc cgtcttttcg cgcagctgct cgatatcaga taaatgcgtc
     9781 tgcatgtatt tcgtgatgct ataatcatta tcgttttttt ctaaagaaag aacgattttt
     9841 gctgtatcat cattgaatgg gatgccttca tactcttggc ttgcttggat ttctagtggt
     9901 tcttcttcta aatgagaaaa gtttttcgtg tacacataat cattaccgaa taaaatattg
     9961 tctttgttag ataaaaaatc ttccaaggaa ctactaattt tatctggatc gatcacgttc
    10021 ttttcagcat ctgaactgga taaagatttc gtcaaaacat caccgtctaa aaacacacca
    10081 cttgccagca agccgctttt accagccttg cgataattcg ctaatgaagt gctaagattg
    10141 gtctgctccg cgctaagata atagccttgc ttgttctcag acgaaatctt ccccgtgtaa
    10201 gtgatatcgt cgctagccag acgttgttgg attgattcag tctgattaga gcgaatgaca
    10261 ttgttttctt ggtataccgc gtcatgataa ctgctgaata aaaacacatt caaccctaaa
    10321 aaagcgacga aaaatatcca ttcgattttt ttgaaatcca attaattcgc ctccaatcca
    10381 ggtaattttt ccagaaggtc gttagctgaa taccagttat ctttatattt cacataccat
    10441 tcaggtaaga gatcaacgac acgattcgtt tctttgacat tttgccatgt atagccaacg
    10501 atcactgaac caatcttgtt gctatctgct cccttagctt ttaactgctg gatgatttca
    10561 ttcgtacttg caagttctac ttcttcgtct gaaggtatcg gcacttgaat cgtattcaaa
    10621 ctggtttgga tattcacttg cacagaacca ttcgtttcac cgccaacttc cacaccaatc
    10681 tgtcctctgc tgtctgtccc aaaaaccggg aagccttcca cgaatatccg ataatcgatc
    10741 gtattgtcac tgcggtcaaa atatctgagg ttccctaaag aactgcctaa tttgctaata
    10801 taggggaaac tttcagaaaa tacactagca ttttcttttt tctctggcag ctctccacga
    10861 aaatcaacta cttgcgtttc cgaatggata gacattgttt cattcccacc gtaaaaaatc
    10921 aattcattgc tttcatcatt atttttcacg ctatctggtt tctggaaaaa agcatttcta
    10981 aaaagtgtat agggttgctg tgacaggata taactatact tcttcagttt gatcgaatgt
    11041 ttcgtagcat attgcgtgtc gatcgtcgat ggttctgttg tcatttctgt ccattttgtg
    11101 tcttttgctg ttaattcttt attgatatct gtccaattca acgaaatatt tgcttcatat
    11161 acgagatggt tcgaataatt caaaaatcgg atttttttat ttccttgatc gaattgtatt
    11221 ctagtaaaat acatattttc gctttctttt gccaagttcg acacatctaa atctaaatca
    11281 aatgcttcct tgtattctct tagcaaaaaa ggcgcattat aggccaactc aaaaccagag
    11341 ttcagatttt tcacctgttc aaactgtgat ttgtctcctg aaactacttc agaaagcttt
    11401 ccaaatcttg ctttatcgat gatcaattgc aatcggtaca cgagattttc actgttggtt
    11461 tcttggatca ttcccgaatg gtgccatgta acatgaagcg gaagaaagac ttcggaagtt
    11521 tttcgattgc tttgagactc gatcatttga gtgtttgtct catctacgtt gattgaactt
    11581 tttcctgctg ggctcagcca gattgcataa gagaagtata tgcttaacgc aaccatcaaa
    11641 atgagtccta tacgaacgac tttttcagag attctcattc ccaccattcc tcctcataag
    11701 gttcatatgg cagcgagata taaaaagtcg acccttttcc ttccttgctc tctgcccaaa
    11761 tagcgcctcg atgcgcctta atcacttctc tagaaatagc taatccaagt cctgtcccac
    11821 cttgtgctct tgcacgtgct ttatctactc gatagaaccg ctcgaacact cggttcagat
    11881 cttttttagg aatccccaat ccttgatcgg tgatacttaa aatcacgtta ttatgcgttt
    11941 ctaataagcg acaagtaatc gttcccccat ctggtgaata cttgatcgcg ttattcatga
    12001 tattatcgac tacttggatg attttatctg tatctacttc gacccaaaga tctctttgcg
    12061 taaattcacg acggatctta taattttttt cctgatttcc caccatcata tcaaaccggt
    12121 caagaacaaa attcactaac tcgttgaaat tgacatattc taattgaagc tgggtattac
    12181 ctgtatccat gcgagataga tttaagaggt cattgatcat tcgaatcatt cggtctgttt
    12241 catctagcgt taccttcaaa aaatttggtg ctacttcctc gtctttccac gcaccttcgc
    12301 tcaatgcttc gatataacta cgcatacttg tcaacggtgt acgaagttca tgggaaacat
    12361 tagagacgaa ttcacgacgt tcccgttctg ttttttcttg ttctgtcaca tcatgcaaga
    12421 cagctactaa tccgctgata aaacctgatt cgcgacggat catagaaaag tctactcgca
    12481 agatcatctg atcactttcc aatgtactgg ttgaacggtc aagtaccatt tcgtttggat
    12541 cttctaataa tttccgcaac gtatattctt cttcgatatc taaaagtgtc aaaatcgatt
    12601 gtccgattgc ttcctcgttt ttgatatcta gcaaggaaac agccatatca ttgatcgtaa
    12661 tgactttacc cctgcgatct gtcgcaatga cgccgtctgt catatgggat agcacgctat
    12721 ccaaacgatt gcgttcagct tccattgtat cttgagcttc ttcaatacgt tcagctaact
    12781 gattgaaggt atcagccagc tgtccaagtt catcttgtcc atgcactttg actttccgac
    12841 tgtagtctcc tctagcgatt cgaatggctt gttctctcat ttcaccgatc ggctgtgtaa
    12901 tcgaacgagc aactaaaaca gatacgatca ttgaaataaa tgccgcaatt agtgaggcag
    12961 taaagaaaat gactgctgtg ttattgattt ccgaatattt ttgttcgata ttacttttga
    13021 cgtaaagagc cccgataacg gcatcgccgg tcggggacag gatcggttga acattgatat
    13081 tcacccgttt atcgtcgtta tctaaagcaa cataacgttt agtcgtgaaa tcattgatat
    13141 caacataatc atttttcttt cccacagcgc tttgttcatt gacatcggtt gttcctcgga
    13201 tgatcccttt ttcgtcaacg acccgtattt cgatgatatc gctagtcgca ctgttttcaa
    13261 taaatctttt gatattcgca tttacctctt catcatctgc attatctttt cgattcaatt
    13321 ccgggctgat tgttgttgct aatgattcca ccgtttgatt catatcttta ataaatgtat
    13381 tgatcgtgga acgttctaaa ccacgtataa agtaggcacc aataatttcg atcgagatca
    13441 ataaaatcag aataaaggta atagcgatct taaaattgac tgatcgaaaa aagcggactt
    13501 ttcttttcat ccaattactc ctgttctggg ttacgcaaat agtaaccgac accacgacgt
    13561 gtcactaaat aatttggatg gcttggatta tcttcgatct tttcacgcaa acgacgtaca
    13621 gttacatcca ctgttcgtac atctccaaaa taatcataac cccaaaccgt ttgtaataaa
    13681 tgttctcttg tcatcacttg tccaaggtgc ttcgccaagt aaaagagtag ttcgaattca
    13741 cgatgtgtta actcgattgt ttctcctcgt ttcgtcacca tgtaagcttc tggatggatc
    13801 gtcaaatcac caatcgtcaa ttccgcaggt gctgcctctt ctggttcctt cgcagccgtg
    13861 gcaccgcgac gaagatttgc tttgacacgt gcaactaatt ctcggttgga aaatggcttg
    13921 gtcacgtaat catcggcgcc taactctaag cctaggactt tatcaatttc agaatctttg
    13981 gctgttacca taatgatcgg catatcataa gttttgcgta cctcacgagc aacttctaag
    14041 ccgtccattt taggtagcat caaatcaagt aaaatcaaat caggttctac ctcagcaact
    14101 ttttccaagg cttcttcccc atcatatgca gtataaacgt catagccttc tttggctaaa
    14161 ttgaatttga cgatatccga aatcggcttc tcatcatcga caactaaaac ttttttcata
    14221 aagagccacc tcttatatat tattgtcata ccttctcatt ataccgtatt tctgcttttg
    14281 tttcatcttt gcctataaaa caaaagagaa aattaaaatg aaagtgcaaa atactttatt
    14341 attttttgtt ctaacttcaa taaaaaagga aaacactgat tttcacctca gtgtttcctt
    14401 tttttgattt atccttctaa aacttgtaca ccgacaccca ataatcccgg accagtgtgg
    14461 actactaatg ccggagaaat ctggccaaag tagatttggt ttgcttgtgg gaactctctt
    14521 ttaagtctcg cttccatttc aattgcttct tctttcgcat ccccatgggc aacagctaag
    14581 ttgaagacct ttgcattacc gattttttct ttgacgtaag caaccgtttt atccaaactt
    14641 tttttacgtc ccctagcttt tcctactgtg taataaatcc cttcttcatt acatgagatg
    14701 attgggttaa gctttaaagc ggttcctaca atagaagcaa ctaaaccgat ccgtccacct
    14761 ttttgaagat attccaatgt cgcaacatta aagaaaactt ttgttctttc gactaactca
    14821 ttcagattac tgatcagttg atcccattct actccttctt ccagccattt tgctgcttgg
    14881 atcgcagaaa aacctgcacc aataccaata tttttcgtat ctagtacata tgcttccaat
    14941 tcttcttgct gttgtgcaat taaacgaacc acattatatg tcccgctcaa tccactggaa
    15001 attgttaccg caagtacctt ttcataaccg tcttttttga tttgctcaaa gatttctgtg
    15061 atcgactcac catccggcaa agatgtacca gggatttcct ctggcaaacg ttgatagatc
    15121 tcttcagggg taatgtcaat tttatctgta tatacctgat ctttataaat aattttcaat
    15181 gggatcatat acatctcgta tttttttatc aattcttctg gaacatccgt acctgaatct
    15241 actaaaattg cgattttatt gtttttcatt tgttttctcc tcttttaaat attcggtttc
    15301 tagaacaatc gttttttccg caagaagttt cattgcaaac gataaagttg ccgctcttac
    15361 agcaatatat tctacagggg ttgcctggtc gaatttttgc ggatgagatt taccttgtgc
    15421 taattggctg accatccaca cagctgcttc ttgttcatcg caaaatagat tataggcttc
    15481 acgaattcct actgtttttc cttggaacaa tatcccttct ttgatttcag gaatagtcaa
    15541 tacttgtttc aacgtagtga ttgcaagtaa aaatgctacg tgttctttgt tatatctttt
    15601 tttaactggt gctggtatca aacctagttt aacgtaattg tttaccatag aagaggtaag
    15661 caatggatgc ttttctcctt gtatcacagg tgaaagataa cgatcaacta acgttctgac
    15721 ttggtccata taaagttcta agtcagggag ttcttcccag tgaggcagac ggaatgtttc
    15781 tagttccttc ccccactcca ttaattgctg acgcattttt tccatcataa ccgctccttt
    15841 tttcatttca taaacatgat atcagactta tgataaacat tcaatctagt tgctttaact
    15901 aggtgatttg attagcggga ttccatgaaa acaaaaaaca agaaggctaa gccttcttgt
    15961 cttttatcgt taagaacagg agacccgccg gggagacggc gggaaaggag taaaaaatga
    16021 aaaaagtgtt agggtttgtt ctcttgcaag aactttgttt gttggtatgc ttatataata
    16081 gcgagcaaat gtgaataatc tatgaatgat ttcctataag gaagtaaaga tttttctgat
    16141 tttctttaaa tgtagtcttg cattttaaaa ttcttagtgc tactattatt aagtactttt
    16201 tgtacgggcc gttagctcag ttggtagagc agctgactct taatcagcgg gtcgcgggtt
    16261 cgagcccctc acggcccatt gggtgccaaa cccacggaac aggtaaatgt tggtgcgaaa
    16321 gcatttcgaa aacatttacg ctgttctttt tttatttttc aaaataacta gggcttttta
    16381 tttttaactc aaagtccact gtagcccttt ggactttgag ttaaaaaaga agcataatta
    16441 tgaacagtaa agtttctaat ataacaataa ttcgagaagc tttgcaaaat catgctgcca
    16501 atgaactctc ta
//
LOCUS       DASJUF010000056        16454 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJUF010000056 DASJUF010000000
VERSION     DASJUF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.056"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..584
                     /gene="scm"
                     /locus_tag="U4B62_001719"
     CDS             <1..584
                     /gene="scm"
                     /locus_tag="U4B62_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN2141879.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSD
                     STDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSD
                     SSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPK
                     TGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(739..1725)
                     /locus_tag="U4B62_001720"
     CDS             complement(739..1725)
                     /locus_tag="U4B62_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2141880.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1748..2314)
                     /locus_tag="U4B62_001721"
     CDS             complement(1748..2314)
                     /locus_tag="U4B62_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN2141881.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2328..2744)
                     /locus_tag="U4B62_001722"
     CDS             complement(2328..2744)
                     /locus_tag="U4B62_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2141882.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3201..5135)
                     /locus_tag="U4B62_001723"
     CDS             complement(3201..5135)
                     /locus_tag="U4B62_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2141883.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5416..6372
                     /locus_tag="U4B62_001724"
     CDS             5416..6372
                     /locus_tag="U4B62_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN2141884.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6462..7565
                     /locus_tag="U4B62_001725"
     CDS             6462..7565
                     /locus_tag="U4B62_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN2141885.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7578..8531
                     /gene="menA"
                     /locus_tag="U4B62_001726"
     CDS             7578..8531
                     /gene="menA"
                     /locus_tag="U4B62_001726"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN2141886.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9345..10691)
                     /gene="gor"
                     /locus_tag="U4B62_001727"
     CDS             complement(9345..10691)
                     /gene="gor"
                     /locus_tag="U4B62_001727"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN2141887.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10825..12078)
                     /locus_tag="U4B62_001728"
     CDS             complement(10825..12078)
                     /locus_tag="U4B62_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2141888.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12084..13007)
                     /locus_tag="U4B62_001729"
     CDS             complement(12084..13007)
                     /locus_tag="U4B62_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2141889.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13228..15720)
                     /locus_tag="U4B62_001730"
     CDS             complement(13228..15720)
                     /locus_tag="U4B62_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN2141890.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15727..16194)
                     /locus_tag="U4B62_001731"
     CDS             complement(15727..16194)
                     /locus_tag="U4B62_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN2141891.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16330..16403)
                     /locus_tag="U4B62_001732"
     tRNA            complement(16330..16403)
                     /locus_tag="U4B62_001732"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16367..16369),aa:Arg,seq:acg)
BASE COUNT         4851 a         3452 c         2926 g         5225 t
ORIGIN      
        1 acacgtcagc ttcaagcgac agtacagata ctacctctac cacaagtgat agcacggaca
       61 catcagcttc aagtgacagt acagatacta cctctaccac aagtgatagc acggacacgt
      121 cagcttcaag cgacagtacg gatacatcta ccacaacaag tgatagcacg gacacgtcag
      181 cttcaagtga cagtacagat actacctcta ccacaagtga tagctcagat tcatctacca
      241 ctacaagtga tagcacggac acgtcagctt caagtgacag tacagataca tcctctacca
      301 caagtgatag ctcagattca tcaacttcaa gcgatagtac agacacgtcg tctaccacaa
      361 gcgatacacg tcatgcaagt gtctccttct caaaaacaag ttcggataac aggacgaact
      421 ttaacttaac taataaaaca agcaatgaca atggaaacaa atcaaaaaga gcacttccta
      481 aaactggaag tcaatcgaat aattggataa cactcgcagg cgttatatta cttgtaatcg
      541 gtttaagaat cacttttatg agttataaga aatcgaaaag gtaaaaaaat attcaagata
      601 aaaaagagca acgtcgctaa cggacgttgc tctttatttt tgctttttat cataaataca
      661 agaactacaa ttcaatttgt caaaaaacac gtagatttcc ttctgagaaa tttatttacg
      721 tatcgaacct ttccctattc agtctttgcg atccaaaatc agttgggtaa gggaaaagag
      781 attctctttt gttcgaagag aggtggcagg taatttcttt atttccttca aagccttttc
      841 tgtgtaagag gccgctaact tcctagcttc ttccacgcct cctgtatgtt caacgatttt
      901 gcgtacggcg tcgacatctt cttctgtcat tgccgctttc ttctctaaat aaggtagtag
      961 ctttggtttg tttttttgca aagagtagat caacggtaaa gaatagaccc cttgacgcat
     1021 gtcttctagt acaggtttgc cgatttcctg gctctctttt gtataatcta aaatatcatc
     1081 gataatctga aaggccatcc cgatagaaag tccgatatct cctgcttttt ttgcgaagcg
     1141 ctcgcttgtt ccactttcaa aagcgccaac agaacaactt aattggaaca gttcagctgt
     1201 cttacctgaa atattttcca aatattcttt tacagaaagt tcataattat agcgatcatc
     1261 catctgccct aattcgccat tcaatacttt ttccatactg cgagaattca attggagact
     1321 tttcaaggaa gtggagtaat cagacaatag tttaaaacag cagacaaata aatagtctcc
     1381 tgcatagaca gaaacgacat taccaaactg actacggata ttaggctgac cacgtcgcag
     1441 atccgcttca tcaacgatat catcatggat cagtgtggcc gtatggagca tttcgataga
     1501 agctgccaaa gcgatggctt tgtctcgatc ttgctctggt ccaaattgtg agaacaatag
     1561 ctgataggca ggacgcaaca gctttccgcc ggcatggatc gtttctttga ttgcttgttc
     1621 gacaggtttg tttttcaggc ggacagtttc ttccatccgt tttagtgttt ctgtcaattc
     1681 tttacttaaa gcaggatagt tttcccacat tggatgtagc ttcataaaaa aactccttgt
     1741 gtgacgatta ttttttggta aacattgggc tccatttttg tttggttccc tgctcataat
     1801 ctttttggaa tcgttgaagt gtctgcacat gtcgtaaaag ataattcgct gcgatcccga
     1861 ttgcaatccc tgataaaatc cctaaaactg aaaggactgg caagtacaac atgactgtcc
     1921 atgattgtgc aatgaaagaa gccatcaaaa gctgtccgac attatgcatg aaaccgccgg
     1981 ccgcactaat tccgatgata ctgacacgtt ttggtcctaa ttgcttgacc aataacattc
     2041 caaaataact aagcaaagca ccactcatac tataaaggaa tgtggaaatc gttccgccta
     2101 gcaaagtagt caggaatagt cgtagccaaa gtagtaagaa gctgtctcgt tttttcatgg
     2161 taaaaatggc aacgatcgtg attagattgg ctaagcctag ttttgcacca ggcgcaaaag
     2221 aaaaaggata agggatcata ttttccaata aaccaatgat cactccttgt gcaacaagta
     2281 gcgaaatata aatcattttt tgtaatttac tcatggtgtg tgaaggtcta ataaatcaga
     2341 ctgtcttcgt cacctccatc tgttgaacgg acttcgatca ctaatttatg tggcaaacag
     2401 acgatggttt cgccattttt cgttgcccag cctcgacgta cgcagatctg gtcatcgcaa
     2461 tctgcttctt ttatccgtac tcgatcgcca tctatctcga tcaagttata atctccgtgt
     2521 tcatctttgt aagtatatgt gttgctctct tctccagcca tcaaagggaa tgttttgatt
     2581 tctgtgccat ccacacgcag taccgcttct ttttctgctg ttgcggtgtt ttgctgaaga
     2641 ccaaaaatca tgactggtaa aaaagaaaga acagtcaaaa gcaaaataat aaaaacatcc
     2701 caaggtttga gcagactttt ttcagcaaat tctttaaggc tcatttttgt tctccttttt
     2761 ccagattaac tcacccaatt ttagcataaa aacaagaggt tgtgacacct gtgtcttgct
     2821 ttgagtaata agaacgaaga atagaaaaca gctttccatg ttttctaatt ctttgggctt
     2881 attaccgaga agcagcaggt ggaacaccgt ttataacaag aggttgtgac agaagtgggc
     2941 aacttcaaga aataagaagg aatttacgaa aattactttt caagtttttg tgaaatttcg
     3001 gcttattttt cgaagaagct acttctggaa caccgtttat aacaagaggt tgtaacagag
     3061 gttgtgacac ctgtgtcttg ctcatggagg cttatctcgg gcatcttatt cctttaattt
     3121 tacataaaaa ggaggctgtg acggtttgtc tcagcccctt tctattgaaa tcttgcattc
     3181 aaataacgtt ctttatctat ttatttcttg ccgtactggg aacttggtct accgtaccac
     3241 caattaccta gtgtacgttg tagccatgga attacccagc gatccaaccc aatcgcacgt
     3301 ccggaaccgt tcatcaatgc gaaagcaaca aaaatgaacc aaatgtttac ccagtaaaac
     3361 atgcctgata agcagaacgt gatcaccaat acaatcgttg tcgcactgca tagccatgtg
     3421 aataatccag caatcaatgc taatgccaag gcaatttcaa agaataccat gaatttttgg
     3481 aagaacattg ctacatcatt gtttggcatg atcacttgca tgatctttgt gaaccagtct
     3541 ggtgacttct caatcacagc cattggttct tcaccgtatg cataactcaa accaaaatgt
     3601 gcagcttgag tcgctgcttc tccggcactt gcccctgctc cgctggcagc tgtggttgca
     3661 tcaccagcag cagaagcacc actggcaaca tctgttacat tgtattccca tgtccaaggg
     3721 aacacactgt ttccagtgaa ccatgatcct tcaccaaacc aagtacttgg tttgaacact
     3781 tcaccattcc caatgatttt tttggaagct tcaacgaacc aaactaaacc atagaaaata
     3841 cgaagtggca cactccataa gacattgccg taacgagaag aatgtcctcg agtcacgttg
     3901 cgatcatctt tgatatggaa aaactcatgc attaaatatt ggaacatata ataaccagaa
     3961 cgaatatcaa agaagtattt caaattcacg atatgtttca ttacgatagc taaaaatccg
     4021 cttaaatgga tcttatcaaa taggttagct accccccatt tggcaccaat agaaaccata
     4081 aatccttgat agtttccttt aaatttatgc ttttctccgc ctttgatcga agccacgata
     4141 ttcgctgcgg ctgtgtgtcc tgtttgttca gcagcttgaa cgatctgcgg tgtcggtgtt
     4201 cctgggaatt cttcataaaa gactaaatca ccaatgatat agatgttttt atcttcgtag
     4261 ccttttgcct gcatatattc attggcgatc aaccgctgtc cgcgtgctga ttctaatcca
     4321 aagtcagcag cgtcagaagt tcctttgaca ccagctgtcc aaatcagtgt gtgagtcgga
     4381 atgttgcgtc cgtctttcaa tttgatatga tcagcagcca cttctacgat tggtgcattc
     4441 aataataatt ccacgttgtg cttacgcaaa tagcgttcag cttttgcagc atcatttcga
     4501 tccaacatat tcaagatcgt cggcattgcc tctacgacca tcagtgtgat ttcgtttgga
     4561 tctaatttat aatctttggc taggcgattt ttccagtcga tcagttcccc aatcatttca
     4621 ataccggtaa atccagaacc acagacaacg aaagtcagca tggctttacg cagttcatca
     4681 tctggttcca atgcagcttt tgctactgtc ttctcaatat gttcccgaat ccttacagaa
     4741 tcttcaaatg accaaagggt gaatccgtgt gctttgacac caggtgtacc aaagtcattc
     4801 ggctctccgc ccattccaag gatcagctga tcaaaaggat aggaacctaa tttggtcttg
     4861 acgatttttc gttctttatc gataccgatt acagtgtctg tgacaagttg tacattcttt
     4921 tgacgagcaa acaaatgctg cagatcgtat tggattgctt ctggttctac acgtccgcca
     4981 gctacttcat gcaactcggt catcatagta tggtacgaat gacgatcgat cagagtaatt
     5041 gctacatctt cttttttcag tttctttgcc aagtattttg tagctgaaac accggcatat
     5101 cctgctccta caacgacaat attttgcttt gccattgatc atccgctcct tatagactat
     5161 ctatataatg aatatatgat aattctttta gtttgatact tattacacaa tctccatata
     5221 ttatatagat gttctgttct tttgtctaac tccttttgga tttttttcaa attttaaaat
     5281 aaagcgtttt ttcacgaaat ttgctgaaag aaaacgtttg aaatattaca tgggttattt
     5341 atcaaaaata gttgacttat aggctaaaaa gattagaatg tataatgaat cacaaagaaa
     5401 aattgtggac tagaaatgga atggtggggt attaaaatga agaaaaaacg ttttgtaact
     5461 ggatttgccg tattagcatt ttcagctcta gtgttaggcg cttgcggcgc agatgacaac
     5521 ggaagctcaa actcttcttc tgaatcatct acagcacaat cttcgactgc aaaaagcaca
     5581 acagaatcat ctgcaaaagt tgtagctggc ggagatttgc aagacggcac atataaatta
     5641 gaagaaaaaa attattcaaa tggttaccgt gcagtctttg aaatggttgt aaaagacggc
     5701 aaaatcactg aatcaaaata tgataacgtc aatgaaaacg gcgaatcaaa aacaaaagat
     5761 gcagaataca acaaaaacat ggaagcaaaa tctggcacaa acccagaaaa atacattcct
     5821 gagttgaatg accaattctt aaaagcacaa agcgcaagcg gtgtggaagt cgtaactgga
     5881 gctactcact catctgaaag cttccaaaac tacgcacaac aattgatcca agctgctcaa
     5941 gctggtaaca cagatacaat cgaaatcgac aatggtgctg atttgaaaga cggcacttac
     6001 aagcttgaag aaaagaacta ctctaacggc taccgtgtgc aattcgaaat gacagttgct
     6061 ggcggcaaag taactgaatc aaactttgat tatatcgaca aagacggtaa atcaaaacaa
     6121 gatgatacag aatataatga aaatatgaaa gcaaaaagcg gtacagaacc aaaaacttac
     6181 atccctactc tgaacgacga attcgtcaaa gcaatgggcg aagaagatgg ctcacctgct
     6241 aatgtagaag ttgttacagg tgctacacac agctcacatt cattcatcat gtacgcacaa
     6301 caattagtga atgcagctga aaaaggcgac actcaaacaa tcgaagtaga taatatcgta
     6361 acagaaaaat aatcacataa tcatactaat aaaagttgga gggtgggacg atagccagat
     6421 taggcagtcg tctcaccttt caaaatgaag agaggaaaaa gatgcggaaa aaactactat
     6481 tttccttaat cacactgggc atctctttta tggtgctaac tggctgcagc aactcagcaa
     6541 ataaaacaac cactgaaagt tcagcagcta ccaagatatt gaaagatcca tattctgatg
     6601 aacagttctt gttagggact tacgttcgta tccgtgtgta tgatgaaggc aaagaatcag
     6661 cattgaaacc tgccttcgat cgagtaaaag aacttggtga taaaatcacg atcaatcaaa
     6721 aaggttctga gatcgatgaa gtgaatgagc aagctgggat caaaccagta aaagtttctg
     6781 atgacgtcta cacgttagtc aagcgggcct acgaatatag ccaagattct caaggcggat
     6841 tcgatatggc gattggtgca atcacacaat tatggcgaat cggttttgat gacgccagaa
     6901 aaccttccca agaagaaatc gatcaggcat tgaaattagt ggattatcat aagatcgaac
     6961 taaatgacaa agaaaaaact gtttatctaa aagaaaaagg aatgatcatc gatttaggtg
     7021 caatcgccaa aggttacatt acggacgaag tagtgaaagt cttgaaaaaa caaggagtga
     7081 ccactgccat cgttgacttg ggcggtaatg tctatgtctt aggccacagc ccaagaggcg
     7141 aaaaccaaga ctggacagtc ggcatccaag atccgaacat ggctcgcgga tctgttttag
     7201 gttctattaa agaacggaac aaaacattag ttacctctgg aatttacgag cgttatttag
     7261 aagtagatgg caaaaaatac catcatttgt ttgatccaaa aacgggttat ccgttcgaca
     7321 atgatatcgc aagtgtcacg atcataacag ataaatcaat tgatggtgac gggttgtcta
     7381 cagcagtctt ttcaatgggt gtgaaaaaag gactggaata cgtcgaaagc gaattgaata
     7441 acgggacgga agcgatcttc gtgacaaaag acgataaagt atatgtgacc gatccaatca
     7501 aagacacctt caaattatcg gaggactccc attacacgat gggtgaccgt tctgaactaa
     7561 aataaggagt acgaaccatg acactaaaaa tctttttaga agtagttgaa atacgaacaa
     7621 aggtggctag tgtatttcct tttgcggtag gggtattatt ctctatcgct ttttttcatg
     7681 agatacattg gctaaacact tttttatttt ttattggaat gctagttttt gatttagcga
     7741 ctaccgctat caataattac atggattatg aaaaggctca ttctgaagta tataagtatg
     7801 aagaaaatgt cattggccgc agaaatctat ctcctgtatt gattcgaaac atgattttag
     7861 ctatgattgc atttgttttt gttatcggcg ttattctaac gatcaatacg ggttggcttt
     7921 tacttttgat gggatttatc tgttgtttta ttggagtatt ctatacattc ggaccgattc
     7981 ctttatctcg aatgccttta ggggaaattt tcagtggggt gactatgggc cttggaattt
     8041 ttgccatggt cgtttatgtc aatacattcg gttcagatgt ctatgatctg acagtcaact
     8101 tttcatctgg acaatttgct ttggtaggaa atctttgggc tatagcagca atcgtattag
     8161 cttccttacc cttggtgttt acgattgcca atatcatgtt ggctaataat ttgcgggatc
     8221 ttgaccgtga tattgaaaat catcgctata cactagtttt ttatatcgga cgtcctgcgg
     8281 gaacagtctt attccagcta ttgatgtatg cttgctattt cgtgatttta atggggttgt
     8341 tgagcggtat ctatgaatgg ccgatcttgg tcaccttctt gaccttgcca attgtgaaga
     8401 agcatttgga tcaattcaag caaacgttac cccagcctat cagtttcagc tattccatca
     8461 aaaatatggt cttattcaat gccagttatg tattggggct attactaaca atcattatgc
     8521 aagccattta acagcatgaa gaggttgtga aaaaactgcc ctgcatcaag taataagaac
     8581 agccaaataa aaatagctcc ttatattttt cgtttggttg ggcttattac cgcagatgca
     8641 agcttttgaa caccgtttat caggaggttg tgaaaaagtc gcttagtttc gagtattaag
     8701 aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa taccgagaaa
     8761 ctggcttttg aacaccgttt atcagaggtt gtaaaatcag tgttttgtcc tgagtattag
     8821 agaaacaaat ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaagga
     8881 cagctgattg aacactattt gttcggaaaa agtgactaac tatgacacaa tttttttcac
     8941 agtccctttt ttcacttgat ttgattcgta ccagcgaact tgacgagaac tttcttggtt
     9001 tggtaactat atggtattct tttaacatcc tcgtttgctt cttctaattt atttatacaa
     9061 agtcacactt ttttaaacat ctgttcttcg atacatttct atttctttta aacatcgata
     9121 ccaatatttt gaacttaaga gtgcatacca aatggattca tttactcttc gtcagttacg
     9181 aagataatca aaatctccaa cttccctact tatttcgttt tgagaattat cgaagcaatc
     9241 ttacataaat aactaaaaaa caaaaaacca gaaaatcagt atactgattt tctggtttta
     9301 catggtttgt tcaataaaaa gctgttggaa ctgttaatat taaattatcg catcgttacg
     9361 aattcttccg ctcctgttgg atggatggct actgtattgt caaagtcttc tttagttgca
     9421 cccatcttga tagccacggc aaaaccttgc agcatctcat cgacaccaac accaatagca
     9481 tgtaagccga cgattctttc ttcttttccg acacagatca atttcatttc gcatttttgg
     9541 cgataatcat ttaatgcaaa gtacattggg gtaaagcgtg aacgataaac tttcaattca
     9601 ttttcgccat atttttccaa tgcttcttct tctgtcaaac caattgttgc aattggtgga
     9661 tgcgtaaaga cgactgtcgg aacaagttta tagtcaagat aagaatcttt ctttccatta
     9721 aataaacgtt ctgataaacg tctaccagct gcgattgcaa caggagtcag atctaattta
     9781 ccgatcacat caccaacagc aaaaatatgt tcatccgttg tattttggta tttatctact
     9841 ttgataaacc cttttttgtc caattccaca tttgtatttt ccagacctaa tttttcggta
     9901 ttcggtacac gtccaccggc aaaaagtaca caatcaccag ttaaggtttc accattttca
     9961 aatagcaccg tatattcgtc attgttttta gtaatttctt tagcaatatg atgtgcgtaa
    10021 gtttttattc cagcttcttg gtacatttgg atcaaattgt ccgaaagcat tttatcaaac
    10081 gttctaagtg gtcgttcttt tcgatatgcc cacatgactt cactacctaa accacgaaat
    10141 acacctgaaa gttcagccgc aatgtatcca gcacctaaaa cgattgctct cttaggtaac
    10201 tctgttaatt caaaaaatcc atttgaatca attgcgtatt ctccaccagg aatggtcatt
    10261 ttacttgcac ggcctccagt agcgatcaag attttaggag cacgataggt ttcaccgttt
    10321 acttcgatcg tttgactatc gataaaagta gcgtaacctt ttatagcctc gacatgattg
    10381 ctatctaatc ctctttgata agcaccgtgt agaaaatcaa tatatttttc tcgtctttct
    10441 actagaccgg cgaaatcaac attttttaaa tctgcttgga taccgtaact tgaggcatct
    10501 cgttcaattg tttccaaaat cgtacttgct tgccacataa cttttttagg tacacaaccg
    10561 acgttgacac atgttccgcc caattcattc gcttcaatca gcaaaacttt tgcgccatgc
    10621 atccctgctc tgtttgctga agcaataccg ccagagccgc caccaattac gatgtaatca
    10681 tatatttgca ttttgttcct ccttattgaa gagatcaatc aaaaccttta tttaacgatc
    10741 ctttttctac agaaaacaag tgtaacactt ttcttgaatt ttgtatgcga agatgcttaa
    10801 aacagggata tatcagcttt ttccttattt acgttttcgg atcatatctc caggtttcac
    10861 cggctgtgcg actggcatag ttaaaatcat cattggattt ggtgctcggt caatggattc
    10921 gccttcttca ttatgcataa cttcaacggt ttgactgaaa tgactgaatc ctggaccata
    10981 aaattcgatt tcatctccta cagaaaacaa gtttctctga cgtatcgtag caatctgtgt
    11041 tgcctcatca taagataata cttcaccgac aaatttatat actgggattt tacgacgttc
    11101 accgaataat tgttcgtttt cagttggtgt attgtagtaa aaacctgtag ctaattctcg
    11161 ttgtgctact ttccataact cgtcgatcca ttcttgcttg cagacatagt tttcaggatc
    11221 ttccatataa ctgtctacag ccgctttata gacatttgac accgtagata cataatggat
    11281 cgatttcatt cttccttcga tcttgaagct gtctacgccg ttttcaatca attctggaat
    11341 atgttcgatc attgacatat ccactgcact catcgagaat tcttcttcca cttcgcctgt
    11401 atcagttaaa ctgtttcgtt cttgaccaaa aggcatatcg aacaatccat acttccagcg
    11461 acaagattga gaacatccgc cacggtttgc atcccgcatc gacatatggt tggacaatgt
    11521 gcatcgacca gaataagaga tacacatggc accatggata aatgcttcga tttcggtatc
    11581 cgtattttta cgaatctctg ccacttcctc cattgaaact tcacgagcta aaacgacacg
    11641 ttccaaccct tcttctttcc agaattccaa tgtctcaaaa ttagttgctg aagcttgagt
    11701 ggataaatgg atcggcagac ctggggcttc tgtcgcacaa atttcgatca aagctggatc
    11761 agaaacaatc actgcagaaa taccgacatc acgcaaactg cggaaaaatt cccctgctcc
    11821 atcttcattt ccttcatggg tgaccatatt agcagcaacg tataccttcg catcatgttc
    11881 ttttgcaaaa gcaactcctt cagccatttc ttcataagta aaattacctg cacggcttct
    11941 taaaccatat gcattcccgc caatatatac tgcatctgct ccataatgga tcgcggtttt
    12001 taacttttcc agtgttcctg ctggcgctag gatttctggt cgtttcaatt ttctttttac
    12061 tgtcatttct ttcatcatac tccttatttg atagcagaag gatcaatata aaagaatcct
    12121 gtatctaatt cacggttttc aggatgcaat ttactgattt cgctagtcat tttttccgct
    12181 ttttcttgat cccagctacc ttctaaaatt tgtttttttg cttcggcgaa tagtcgagtg
    12241 atgtttacga atttttcgcc tggagtaaat aatccgtcca atttccatgt acagtaatga
    12301 tgctgataaa gttcattcaa ttcattcgag agattcagat cattgctggc aaaaatatgt
    12361 gtgccatgac tatcttcata aatagaataa tgtgtctctt cttttttagg ttccgagata
    12421 aaaagacctc tttcacgatc tttttgttcg tcttgtttcg tatagttata gtaattctgt
    12481 agcagtggac gtttggattg atggatgcat gtagcaccat acacaagggt ttctacagga
    12541 atatcgagtt tttcttccat cgccttcatc tcttcaaaag gaacttcacg agcaagtact
    12601 gctccaactg cgcctttttt cgcccagaaa ttgatttgac gtgcgctcgt tactaatgtt
    12661 tcagcatcat aaatatacgg tatacgcaat tcaggatttt ttttcatcgt ataaataata
    12721 cctgtatcac caactgtgat cacatctacg tttatttctt ttaaaaaagc caaatattca
    12781 ggtacatttt tcattttttc aggatgaatg atcccattta cagcaatatt cgctttttta
    12841 ccatacttgt gtgctaattc taccaattgt cgctgttctt ctcgtgtaaa tgaagcaggc
    12901 agacgcaatc caaattgctc ttcaccgaaa tatagtgtat ctgcacctgc agcaagcaac
    12961 tgttctgctt gttcaatagt ttctactgtg gttatgagtt cgatcataat tttctcctcc
    13021 tacaacttca taatagtagc atagctctaa aaactttcca atacttcttc tatttaaagt
    13081 cggatttttg aaaattattt cacaaaaaat caaaataaag ttcgatatat tctgtccaaa
    13141 tataaaaaga gattgtgaca aaaatcttgt cacaacctct taatcaagtc ttactattat
    13201 aaaaattcag ctacacaggc caagtaacta tagggaacct actgtttcct ttttaggtcc
    13261 gcgaacattc aatgtgatct taccttttga agcacctact gttacgtggt cacctaaacg
    13321 aatttctccg ccaagcaaag cctcgctcaa tcgatcttca atttcttttt gaagtgcgcg
    13381 acggattgga cgtgcaccat attcaggatc aaatccagct ttcccgatca catcgattgc
    13441 agatggtgtg attttcactt gaatatcttg ttctttcaat cgtttgataa tcgctttgct
    13501 catgatttgg acgattgtat ggatttcatc tttactcaat gaatggaaga cgacagtctc
    13561 atcgattcga ttcaagaatt caggtcggaa agctttcttt aactcttcta agatacgttt
    13621 ttgcatggct ttgtgatctt tagtaacatc ttggacattg aatccgacat ttttttcttc
    13681 tcgaatttgt gtcgctccga tatttgaagt cataatcatg attgtatttc taaagtccac
    13741 ttttctacct ttagaatctg ttaaatgacc atcatccaat acttgcagaa gtaaattgaa
    13801 aacgtctgga tgtgctttct ccacttcatc taagagaatc actgaatatg gtttttgacg
    13861 aattttttct gtcaactgtc caccttcatc ataccctacg tatcctggag gagacccgat
    13921 taatcggctt gtactgtatt tttccataaa ttctgacata tcaacacgga tcagggcatc
    13981 ttcacttcca aacatgactt ctgaaagtgc tttggccaat tctgtttttc cgacaccagt
    14041 tggtccaagg aacatgaatg aaccaattgg acgatcagga tctttcaatc cgcttcgtgc
    14101 acggcggatc gcacgtgaaa ctgctttgac agcctcatct tgaccaacga cacgttcatg
    14161 cagaagtcct tccagttcta acaagcgttc gctttctttt ttctccaact gctgtagagg
    14221 gacaccagtc cactccgata caactgtagc aacatcttct tctgttacac gatcagaata
    14281 accttgctcc tgttttgctt caattgcaga aactttttgc agctttttac gtagtgcttt
    14341 ttcttgtctg cgtaattgag cagcattttc aaagtcttgt ttttggatcg ctgcttcctt
    14401 ttcttgtact agttcatcga tttctagttt gatcacagtt gattttgtta agtgatcggt
    14461 ttgatccaaa cggactttcg ctgctgattc atcgatcaaa tcaattgctt tatcgggtag
    14521 ttgacgagaa gtgatataac gaacagaaag attgactgct gctcgtacag cttcatctgt
    14581 gatctccact ccatggtgct cttcatatct tgtacgtaat ccttgaagaa tcacttctgc
    14641 ttcttcagga gttggctcat ctacttgaat gcgagcaaaa cgtcgctcta atgcagaatc
    14701 tttttcgata tatttttgat attcatctag ggtcgttgct ccgatcgttt gtaattctcc
    14761 tcgagctaac gccggtttca agatattcga agcatcaatt gctccttctg ctccacctgc
    14821 accaattaag gtatgcaatt catcaatgaa taagattact tgaccatcat tatagatttc
    14881 gtcgatgacc tttttcattc gatcttcaaa ttcaccgcga tatttcgtac cagcaaccaa
    14941 agatcccata tcaagcatca tcaggcgctt ttcttgcatg tcttctggca cttcacctaa
    15001 aatgattttt tgagctaacc cttcagcaat ggctgttttg cctacaccag gttcacccac
    15061 cagtacaggg ttattctttg tccgtcggct aagaatctgg ataagccgtt tcacttctct
    15121 cgaacgacca acaactgggt ccagcttgtt ttcttttgct aatttcgtca aatcacgtgc
    15181 taatgaatct agcgtaggtg ttccttgcgg tgtttgccgt ttatcttgta catttacatt
    15241 tctacgacga ttcattcctt ttccagcaga atcaccagct agccccattt tttgtttcag
    15301 aacttgacgg gcttttgaaa ggctgatacc taagttgatc atgattcgag tagccatgat
    15361 actttcatct tttaataagc tcatcaaaat atgttctgtt ccaacttttt gagaaccagt
    15421 tttcttcact tcgctgctag ctaaagcaaa aacttgtttc attcttggtg aatatggaag
    15481 aaagactcct ttagggtaag aacggatacc accataacca atcagatgtt caatttcttc
    15541 ataaatttcc gtttcattca aatccatttc acgtaatact tttccagcaa ttccattttg
    15601 ctcgaccact aaggctagta aaacatgttc tgaactaact gattgatgtt tcaaccgttt
    15661 tgcttcttct tgtgcaattt ctaatactgc ttgggctctt tcagtaaaaa gttcttccat
    15721 ctaatctcac ttcccatctt cataacttaa acgttccaga aatgctcgga gaatccgagc
    15781 acgtgtatga tcttcatatt cattgaaatt caatgtgttt tttgcaattg cactcaacat
    15841 caagtttcct tctttttttg taattatttg gtcttcataa agtttttgaa taatagcaaa
    15901 agcatttttc tcactgattg tatcatcaaa taactgattt atctgctcaa gtaattgttt
    15961 tttatctgaa atccttactt ttgcaattcg tatataacca ccaccaccac gtttgctttc
    16021 tactagataa ccacgttgga tggtaaaacg ggtattaatc acatagttga tttgtgaagg
    16081 aacacagtta aagaggtctg ccatttcaat ccttcgaatc tcgatcattt cactattttc
    16141 cagaattttc tttatatatg attcaattaa atccgaggta ttctgatgtg ccatcttatt
    16201 cattcccttt gactaatatt gaccttaatt ataacgaatt tgataataga aagaaagaag
    16261 aacgcttttt gttaaataaa tcctttagaa tcaacaaaaa aaaatacgga atatactatt
    16321 ccgtatcgac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta gtcaaacact
    16381 ctatccagtt gagctatggg cgcatattca atttaaaaaa attataaatc atttgaaaac
    16441 aaaaagcaag ataa
//
LOCUS       DASJUF010000057        15741 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJUF010000057 DASJUF010000000
VERSION     DASJUF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.057"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..722)
                     /locus_tag="U4B62_001733"
     CDS             complement(24..722)
                     /locus_tag="U4B62_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN2141892.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="U4B62_001734"
     CDS             complement(715..2067)
                     /locus_tag="U4B62_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN2141893.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="U4B62_001735"
     CDS             complement(2290..3690)
                     /locus_tag="U4B62_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN2141894.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="U4B62_001736"
     CDS             3851..4588
                     /locus_tag="U4B62_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN2141895.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="U4B62_001737"
     CDS             4713..5192
                     /locus_tag="U4B62_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN2141896.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="U4B62_001738"
     CDS             5189..5956
                     /locus_tag="U4B62_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141897.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="U4B62_001739"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="U4B62_001739"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2141898.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="U4B62_001740"
     CDS             complement(6859..7461)
                     /locus_tag="U4B62_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2141899.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="U4B62_001741"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="U4B62_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="U4B62_001742"
     CDS             8142..8726
                     /locus_tag="U4B62_001742"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN2141900.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="U4B62_001743"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="U4B62_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN2141901.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="U4B62_001744"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="U4B62_001744"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN2141902.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="U4B62_001745"
     CDS             10627..10806
                     /locus_tag="U4B62_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141903.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="U4B62_001746"
     CDS             10828..11853
                     /locus_tag="U4B62_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN2141904.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="U4B62_001747"
     CDS             12028..13272
                     /locus_tag="U4B62_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN2141905.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="U4B62_001748"
     CDS             13425..14372
                     /locus_tag="U4B62_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2141906.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="U4B62_001749"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="U4B62_001749"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN2141907.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="U4B62_001750"
     CDS             complement(15274..15516)
                     /locus_tag="U4B62_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141908.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="U4B62_001751"
     tRNA            15637..15709
                     /locus_tag="U4B62_001751"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4374 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt t
//
LOCUS       DASJUF010000058        15623 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.058, whole genome shotgun sequence.
ACCESSION   DASJUF010000058 DASJUF010000000
VERSION     DASJUF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.058"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(138..2483)
                     /locus_tag="U4B62_001752"
     CDS             complement(138..2483)
                     /locus_tag="U4B62_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2141909.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2762..3664
                     /locus_tag="U4B62_001753"
     CDS             2762..3664
                     /locus_tag="U4B62_001753"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN2141910.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3875..5650
                     /locus_tag="U4B62_001754"
     CDS             3875..5650
                     /locus_tag="U4B62_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN2141911.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5716..7302)
                     /locus_tag="U4B62_001755"
     CDS             complement(5716..7302)
                     /locus_tag="U4B62_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN2141912.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7553..7777
                     /locus_tag="U4B62_001756"
     CDS             7553..7777
                     /locus_tag="U4B62_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN2141913.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7919..9670
                     /locus_tag="U4B62_001757"
     CDS             7919..9670
                     /locus_tag="U4B62_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2141914.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9670..11454
                     /locus_tag="U4B62_001758"
     CDS             9670..11454
                     /locus_tag="U4B62_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141915.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11789..12641
                     /locus_tag="U4B62_001759"
                     /pseudo
     CDS             11789..12641
                     /locus_tag="U4B62_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12738..14624
                     /locus_tag="U4B62_001760"
     CDS             12738..14624
                     /locus_tag="U4B62_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2141916.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14665..15567)
                     /locus_tag="U4B62_001761"
     CDS             complement(14665..15567)
                     /locus_tag="U4B62_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2141917.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         4794 a         2860 c         3234 g         4735 t
ORIGIN      
        1 tctctggttt ttcacatttt tacataaatt tgtcttaata tcgtagatgt aagcttttga
       61 acaccattta ttcggaaaaa gggagctgtg acaagatttt tgtcgcagct ccctttaatt
      121 ctgattaatc tattagatca atcatcttta tcgtctgatt ctttggtctc tggattcgct
      181 ctagcataag ctgaagcggt gttccagtaa tctgtatagt tttcatcagt accgcctacg
      241 ccgaatttaa aggcgctttt cttcggacca ttttttgccc aaagtgactc cactgttttt
      301 cctggcgttt ggatggattt tttattgaca gtaacttttc cagctgtttg tcctgtgaac
      361 ccagatactt ttttcttcat cacatcggat gataagtcaa atttctcatc agtgccgaaa
      421 atctcagggt ttgcttggta agcttgattg attagattgg caagataatt agccgaacgt
      481 attcttgctt ggctgtccat tccagtattg tcatcgtgtc ctgcccaact actgatagtg
      541 atagatggag ttgagacgat cagccatgaa tctcggtatt cattggtaga accagttttt
      601 ccgacccaat ctgcatttcc tagatttccg tttaagcttg agatgacatc tttgaatggt
      661 gtagtgattt tttcattgat cacactacgc atcatgtcat tcatgatcga tgccgttgct
      721 tcggaataaa tacggacaga attactttca tgtttataaa tcacattacc tgcattatct
      781 gtgatggaat caatgatata gccttcttcg tagactccac catttgctaa ggcctggaat
      841 ccatttgtct gttgaagcgt cgttacatct actgtcccta aaggagccga ttcaacgccc
      901 caattatcat ttgctgggta gttcatcttc gccaagtagt gctcgtaagc gtattgtttc
      961 gatccaccgg aattcaacac atcttgatat aagtgataag ctgggatatt gttggaccat
     1021 tccagtgact cacggacggt ttggaaggtg ttcgaccctt cattggtcgc attgacgatt
     1081 ttctcaccag cattttttcc ttcttgccaa gtcgttgcat aatctgatac acgggattct
     1141 gatccgacaa gtcccatatc gattgcagga ccgtaaacaa gaaccggttt gatggatgaa
     1201 ccagcttgac gttctgtatc aaaggcgtgg ttattttgat tctggctgta attccgacca
     1261 cccacgaagc catagatacg tccggttctg ttatccatca atacatttcc agtctccact
     1321 tggctaccag caccatcatc taacatgtag ccataatttt ctacgccggt ttgtaatgca
     1381 gcatagatat ctttatcgat cgtcgaatgg atcgtatatc ctttgttttg gatcgtttgc
     1441 tgtgcttttt gataataagc ttcactgacc gatgaatctg acatatcagc attatctttt
     1501 tctgccaatt gttgtgcaat aatccttgtt gcttcgttca tcacggtata atacaagaat
     1561 tctctgtcag tctgttccgc gatttgttgt gggagaaagt cttttgtaag atcataggct
     1621 ttcgcttctt cgtattgttc tttcgtaatt tgtccttcac ggtacatact gaataaaaca
     1681 aaatcttttc tggcaagacc ggcagaaaga tcttctttta gcgcgcctgt attcgtgtat
     1741 ggactatacg tgatagggct ttgaggcaaa ccagcgatat aagcagcttg tggcagagtc
     1801 acatcctttg catctacacc aaagattcct ctagcagctt cctggactcc agcaatattt
     1861 tgccccttat tattccgacc aaatggtgag acattcaaat acgtagtaat gatctcattt
     1921 tttgaaaagt gtttttctac ttgtgcagaa agaacgatct cgtttgcttt acgtttgaat
     1981 gttgtttcat cggttaggat ctgttgtttg actaattgct gtgtcaaagt ggatcctcca
     2041 gacgagccga tgccagtcgc ttctgaaact agtgctcgta aaacagcttt agggacataa
     2101 ccttgatgtt tatcgaagta ttcatcttct gtactgataa tcgcggtttt caataaaggc
     2161 gaaatcttat cagatgaaat agtcgtccgc atcagatctg tctggatttt tgatatctct
     2221 gtattatctg cataaacaat ctttgacgtt tccgtaatgt ttcctagttc tgtctgcagt
     2281 tcttttttag tcggcaattg tgtgtcttcg acaagatagg caaagtagcc agcacctatt
     2341 ccgataccta gagctgcgaa caacaaaaga agaacagtaa taaaaacagt caatgattga
     2401 aacactctaa aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt
     2461 tttcgagtat ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt
     2521 atagcaaata ataggacaaa aaaacagaca aggctgtttt tgacaggaat ttctttttcg
     2581 aatgagataa aacgtgagga aatgatcttt tttatgaaag agttcttaag cttttcttta
     2641 gggaaagttt cagttactat ggagaacagg gcaaagaaaa acaaaccatt ttctttgtac
     2701 atcatcttat caatatacag gaattgttcg aaacaattgc ctttcaagga ggaatccatc
     2761 catgttatta ggctcacatg tcagcatgag cggaaagaaa atgctgttag gcgcggcaga
     2821 agaagcagcc agttacggat cttcgacctt tatgatctat acaggcgctc ctcaaaatac
     2881 tagaagaaaa gctatcgaag agatgaatat tgatgccgga aaagcattta tggcagaaaa
     2941 cggactaaca aatatcgtcg ttcatgcgcc ttatatcatt aatttgggaa atacgatcaa
     3001 accagaaatg tttccatttg ctgtacaatt tctgagagaa gagattgtcc gtgcggaagc
     3061 tttaggtgct actcaaatca cccttcaccc aggcgctcat gtaggagcag gggcagaagc
     3121 tgggattgct agaattattg aggggttgaa tgaagttttg acaaaagaac agacggcaca
     3181 gatcgctttg gagacgatgg cagggaaagg tacggaaatc ggtcgaacat ttgaggaatt
     3241 agcgcaaatc atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgatacatg
     3301 tcatacgaat gatgccggat ataatataaa agaagatttt tcaggtgtaa tggaagagtt
     3361 tgataaggtc atcggattag atcggcttaa agtactgcat atcaatgatt ctaaaaatcc
     3421 acaaggttct cataaagatc ggcatgccaa tatcggtttt ggaacaattg gatttgacac
     3481 attgaacaaa atcgtccatg atccaatgtt tgacaacatt tcgaaaatat tagaaacccc
     3541 atatgtcggt aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tttccatgtt
     3601 caaagcgcaa gcattcgatc ccgaagtgtt ccctgagtta ttagcagaag aagtcacact
     3661 ttaagttttc tacctgacag caacaaaaaa ggattgggca gtctgccttg tccttttttg
     3721 tttaagaaac aaaggagtgc tttttgcatt cgttttcgcc atgctccttt tgccccctct
     3781 ttttaaattc agacaaacag ggtatactga tagaaaagta aaaaacgaaa agtttcacaa
     3841 acaagtagca aaacgtgatt gaaaggacga aaagatgctg aacattttaa accccttgat
     3901 cgaccaacta aaaaagaaac atacatactt tattcttggc atagccgtat tatttacaat
     3961 tattcggttg tatttatata gtaaagcaat ctatggaaca gatatgtcgg cgacctatga
     4021 tgatcagctg ctgattcact atgctcaaaa tattttggat ggtaaatggc taggggagta
     4081 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
     4141 actgcatctt tcttatgcag ttttgttcgg catgttcagt atttttgcca gtatattggt
     4201 tgcactcgcg tttaaaccga ttgtgaagaa caagtatctt cttttaagta tctattttta
     4261 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
     4321 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
     4381 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
     4441 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
     4501 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
     4561 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
     4621 tcctatagtt ggcttttcca tcgtctatca gtggatcgaa tataaaaatg agacaacata
     4681 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
     4741 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
     4801 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
     4861 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
     4921 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
     4981 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
     5041 caagggaacc atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
     5101 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
     5161 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
     5221 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
     5281 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
     5341 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
     5401 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
     5461 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
     5521 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
     5581 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
     5641 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
     5701 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
     5761 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
     5821 gattacataa gcgatgtgat cagccaatgc tagttgagcg gaagtggctt gctcgccgaa
     5881 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
     5941 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
     6001 gctgttggtc atcatcagta aaggaatcat cgaaaatggc aacgcaaaag ccaagaacac
     6061 ctgggattca ttcattaagt tattcaaagc ggcatgttct tcgatcgttc cttttgtact
     6121 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
     6181 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
     6241 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
     6301 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
     6361 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
     6421 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
     6481 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
     6541 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
     6601 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
     6661 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
     6721 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
     6781 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
     6841 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
     6901 gatgtctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ccaatgtctt
     6961 acttgtccgt gctcggattg cttgggccaa atccatttgg gtcacgattc ctaattttgc
     7021 tgccatatac tgtaaaagca tagcaattag actagacatc aaaataactg acatcaataa
     7081 atattggaaa ttttgtccac cagtgatcga agtggaccag ttacctggat ccatataacc
     7141 aactgctact aatgcacctg gtccagagta agcaagaagt gttttaaaaa aactttttcc
     7201 ctttggtaca tccactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
     7261 aagtcgatgc ttatgaaatt gtttgttgga ttgattattc aagaaaaaac cacctttcct
     7321 atttatctac gattacattc tacacctcag aaagaaaaaa ttaaagaaaa agattcggta
     7381 tgcctaattt tttttcgtgg tgtgtcttgc tggttaatga aagaaatttt gttcttctct
     7441 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
     7501 ttagtaaaat aaatcattga gagcaattcg gaaatagaaa ggagagattt atatggtatc
     7561 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
     7621 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
     7681 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
     7741 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaaa gactttttgc
     7801 gggctgattt tttcagtccg ttttttattt cgtaatttgt tagactattc agactattca
     7861 gcaaaaaaga caagcacaga caatcctatg ctcttgataa agacaaagga ggagtaaaat
     7921 gtcgattttt agaaaattag gttggttttt tcgtcaggaa aagaaacatt atttaattgg
     7981 gattttctcc ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
     8041 catcattgat gagatcgccg ataagaattt atcaataaag atcatttcct ggtggatctt
     8101 attattgatc cttgcagctt tcttacaata tctttttcgt tatatctggc ggacgaatat
     8161 ttggggaagt gcagctcggt tggagagaga acttcggcag cagctattca aacattttac
     8221 agaaatggac aatctctttt accaaaaata tcggactgga gatttgatgg ctcatgcaac
     8281 gaatgatttg aatgcaatcc agaatgtagc aggagctgga atcttgacct ttgcagattc
     8341 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
     8401 actgattgcg ctgattcctc tgcctttgct agccgttact tcacgagtgc tggggtcaaa
     8461 actccacgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
     8521 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctcgc
     8581 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
     8641 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
     8701 tttaggtgga aagtatgtct tggaaggcac gatctcaatc gggcagatgg tctcatttat
     8761 tagctatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
     8821 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa agacgcatat
     8881 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
     8941 ctcttttaac tatccgaaag aagaatcacc agcattagaa caaatcaaat ttgcgatcca
     9001 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga caatcatcaa
     9061 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
     9121 caaagaatat tcattgaatg ctttattagg atcaatcggt tatgtgcctc aggatcactt
     9181 cctgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
     9241 agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattgccaaa
     9301 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacagag
     9361 gatttcgatt gctcgagcat tgatcaaaaa tcctgaattg ctgatcttag atgatgcact
     9421 ttctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
     9481 agagaaaaca accatcattg cagctcaccg tttaagcagc gtgatgcatg caaaagaaat
     9541 cattgttgtc gatcaaggga aaatcattga acgaggaacc caccacgagc tgcttgcatt
     9601 aggtggctgg tatgaacgaa tgtgggaaaa acagcaatta gaagcgaaga tagaaggagg
     9661 cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa
     9721 cagaagaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaag aacgttcatc
     9781 gttgctatct tatttgcgtt tgccctgtct gtgatcaatg tcctattgcc aaggatcatc
     9841 caaactttta tggatgatca tctggctaaa cagtcagcta cgacacaagt tattttgttt
     9901 tttgctggtg tgtacttgtt tggagtcata gtcaaaagta tcatttggtt tttccaatgg
     9961 tatttgtatt ccatggcttc attgaagacc tatcagtatg ttcgggtaag attatttgaa
    10021 aagctgcata cactaggtat gcgttatttt gaccagacac cggcaggatc gaccgtttct
    10081 cgagtgacga atgacacaga aacgctattt gaattttggt atgtcttttt gatggtattg
    10141 actgggatct ttgcagtcgt ttcttctttc gttgccatgt ttcaaatcaa tggagaaatt
    10201 gccttgtgga ctttggtgtt cttgccgatt ttaggaatcg tcatctggta ttaccagaaa
    10261 ttcagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg
    10321 aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct
    10381 aaagaatttg aacaagtgaa caacgagtat ttagacacgc gctatgcgat gatcaaaacg
    10441 aattcgattc tgttaagtcc aatcatcaat tttctctatg cattggcgat tgcgttggcg
    10501 ttgactttgt ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca
    10561 tttacgacct atgtccagga attttttaat ccaatgtctc aaatgatgga ctttttgagc
    10621 acctttacag atgggatggt tgccggtagt cgtattttga aaatcatgga tacagaagaa
    10681 tatgcaccgc aacaaaatcc tggagcagat caagcgatta cttcaggtaa gatcgagttt
    10741 cgcaatgtaa gcttttctta tgatggagag cacaatgtat tgaacaatat ctcttttgtc
    10801 gctaatccgg gtgagaccgt tgcattagtg gggcacaccg gaagcggaaa aagctcgatc
    10861 atcaacgtat tgatgcggtt ttacgaattt tatgaaggcc aaatcttgat tgatgatcat
    10921 gacattcgag aatatccaat ggaagagtta cgtaagaaaa tgggattggt cttgcaagat
    10981 gccttcttgt tttatggaga tattgcagga aatatccgga tgatgaatcc agctatcaca
    11041 gacgaacaga ttcgccatgc agctgagttt gttcaagcaa acaagttcat cgatgaatta
    11101 ccaaaaggat atcactcaaa agtgatcgaa cacggtgcca gctattctag tggacaacga
    11161 cagctgatca cttttgcgcg aacaatcgtg acagatccta aaatactagt attggatgaa
    11221 gcaacagcaa atatcgatac tgaaaccgaa ggattgatcc aagaaggatt ggcaaaaatg
    11281 cgacaaggac ggacaacgat agcgattgcc catcggctgt ccacgattcg ggatgcagaa
    11341 ttgattttag ttttagacaa aggtcatata gtagagcgtg ggaatcatga aacacttcta
    11401 gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gcagtggtgt gtaaaaagag
    11461 gttgtgacag tagcatagta gcattttgcc ctaagcgcta gagtgaaaat aaaacagagc
    11521 ttcgcagatt cttcatctgt gagcgttagt ggcgtagtca cccgatttaa aaaagacgac
    11581 gacagctagt caaaagcggc taataaccac taagaaagcg ttgacaaaag cgtttgcttt
    11641 cgataatata aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg
    11701 atgagtctcc catggttcca tggacggctt tttgggtttt tctttttgtt ttggatacag
    11761 ccctatatat catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt
    11821 cgtaataacc agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt
    11881 caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac
    11941 aaatgacggg ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt
    12001 agaaatttct tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga
    12061 ttctgtgtat attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg
    12121 aatatttgta ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc
    12181 catcgggaaa aaaaacagtg aagaaaatct cggataaata ccacatcaag ttatcagaag
    12241 atgaagccgg atttatcgca ttgcatttag tcaatgcaca aggcattcaa agccaacaag
    12301 acaataccta cctgttcact ataacaatac aggatattct aaatatcgtc cgctattact
    12361 ataagattac atttgatgaa gattcagtcc atttttttcg attcgtcaca cacttacgtt
    12421 tttttctgac acgcgtccta gatggagaaa aatcagaagt gggagaagtt tctgaggaat
    12481 tgtttcaggt tattcaaaaa aaatataagg atacgtttgg ttgcgttgag aaaatagcag
    12541 actttttagc cgagacgttc aactaccaga tgtccacgga cgaaaaaatg tatttaacga
    12601 tccatactgc gcgtttagtg catgagaatc gttgtaaata aataaaaaaa ggaggataaa
    12661 ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct tccactaata
    12721 aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc gtagaaaatg
    12781 tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt cgtttcaaat
    12841 taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt attgtaacgg
    12901 taatgcgagc gggagggcag tatcaagttg tcatcgggaa tcatgtgcca gtcgtgtttg
    12961 aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca gaaaaaatga
    13021 gaccgtttga tatgcttatt gatggaatat cgggctgttt ccaaccattc cttgctatcc
    13081 tagctgctga cgggatgatt cgtggactga cagcattcct tgtcttctta ggcgcgtttg
    13141 accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta ttccaattta
    13201 tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa ttcgtaggga
    13261 tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg agtggtgcag
    13321 cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct atttatcaaa
    13381 cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt cccattatct
    13441 ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta cctgagatga
    13501 tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta ggttttttac
    13561 tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt gaaacacttt
    13621 tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa attctggtga
    13681 tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct gtgaatggtt
    13741 ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca gcacttaccg
    13801 cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca gccattgttt
    13861 ctggtattgt ggggattaca gagccagcta tttatgggtt tacattgcca cgtaaaaaaa
    13921 tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg ttaatgaatt
    13981 taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat attagaccag
    14041 atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg atggtggtat
    14101 cctttgcgct gacttttttc ttttggaagg aggaagcaga tgaaacagat gatagacaga
    14161 aaagatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct ccactgaatg
    14221 cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg atctaccctg
    14281 aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gtccctgttt ccgacgaaac
    14341 atgcaatcgg gatggtttct gaagcaggtc tggaattgct gattcatgtc ggattggata
    14401 ctgttcagtt ggaagggaaa tattttgaaa gcttggtgca gcaaggagaa agagtgaaac
    14461 aaggcgatct gttgcttcgc ttcgacaaaa agaaaatcga agaggaagga tacagtttag
    14521 aaacacctgt gattgtcatc aattacatgg attatttgga tattttggaa aatcaagggg
    14581 aaacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt aggtgaaaga
    14641 atggactagg cttaactatt ctttttagtc cacttcttta ttttcaaaat tgaaaggctc
    14701 tttcatgatg gctgagctga tagggcgttt atcaaatttt tgtcctacag aaattagatg
    14761 gactaaattc tgttgataat tttctgcaaa aacacaacta tataacacgt ctagaagata
    14821 acaaatcgat gtattgatcg taaaattacc aattttcgaa tacagtcgtt cgcgtgtcgt
    14881 aagtcgcaaa gcggctgtac tcattgaaga tagcgttgaa tcaccgatac tggtcaaagc
    14941 taaaattggc acatgatttt gtttcaatat ttctgcggta cgaaggatca cattgttttc
    15001 acccgtataa gaaatcagaa tcccacacat atcacttgtg caattgtacg cttcatagaa
    15061 cgattcacca aaagtggtga caatcgtgac atccattttg atgcgcttca ttttcagcat
    15121 gaaatcttga gaaatcaaag tattcgagtt actggcgaaa atcacgattt tttttgcttg
    15181 aagcattagt tgtttggctt tttgcagttg gtcatgatga atgagagaaa gtgtatcttg
    15241 aatcgtcatc tgttctaaag aagccatttt atttgcgatc gtcataatac cgtctgtggt
    15301 ggaaaaagga agattcgcat ctacgtcttg aaaattccct tcaagatagg ctgtttcagc
    15361 taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc attttttttg ctacacggat
    15421 gagtgtggac gggtggacat aacatcgctc agcaatctct tggatggtca tttcgccaag
    15481 tgctggagaa tcgcttaaga gaaactgaat cacttcattt tctgaaggag aaaaggagat
    15541 ttctttgatt ttttcacgta ataacatgta gataccttct tcttgttttt ttacgtaaat
    15601 cataactttt ttgaaaatga ttt
//
LOCUS       DASJUF010000059        15166 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJUF010000059 DASJUF010000000
VERSION     DASJUF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.059"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..729
                     /locus_tag="U4B62_001762"
     CDS             <1..729
                     /locus_tag="U4B62_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2141918.1"
                     /translation="LFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRL
                     VKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFI
                     LVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTIL
                     TILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQS
                     VVGFVLLMITNTIARRFDSDSALF"
     gene            741..1661
                     /locus_tag="U4B62_001763"
     CDS             741..1661
                     /locus_tag="U4B62_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2141919.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1702..3159
                     /locus_tag="U4B62_001764"
     CDS             1702..3159
                     /locus_tag="U4B62_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2141920.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3504..4118
                     /locus_tag="U4B62_001765"
     CDS             3504..4118
                     /locus_tag="U4B62_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2141921.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4111..5835
                     /locus_tag="U4B62_001766"
     CDS             4111..5835
                     /locus_tag="U4B62_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2141922.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5832..7283
                     /locus_tag="U4B62_001767"
     CDS             5832..7283
                     /locus_tag="U4B62_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2141923.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7756..9909)
                     /locus_tag="U4B62_001768"
     CDS             complement(7756..9909)
                     /locus_tag="U4B62_001768"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN2141924.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9911..10957)
                     /locus_tag="U4B62_001769"
     CDS             complement(9911..10957)
                     /locus_tag="U4B62_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2141925.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11107..12396
                     /locus_tag="U4B62_001770"
     CDS             11107..12396
                     /locus_tag="U4B62_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN2141926.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12409..15111
                     /locus_tag="U4B62_001771"
     CDS             12409..15111
                     /locus_tag="U4B62_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN2141927.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         5109 a         2648 c         3052 g         4357 t
ORIGIN      
        1 ttattttctt cggatcaagc atttctcatc actaaaaata caattttata taatgtagca
       61 tttatccttt tgaatctgct gatctcagtc gtatttgcaa tcattatgag cgagttgaga
      121 aataaacgac tcgttaaagt ttatcaaacg atgtccttac taccttattt tctatcttgg
      181 gtcattatca gttactttgt ttacgctttt ttaagtcctg acaaaggaat attcaatcag
      241 tggatcactg gacatggagg agaagctgtc aattggtata atgaaccgaa atattggccg
      301 tttatcttgg tgttcattgg tacatggaaa gggattggtt ataacagcat tatttacttt
      361 gcatctgtta tgggaatcga tccgacttat tatgaagctg ctatggttga tggagctagc
      421 aaatggcagc agatcaaaca tgtgacgata ccacaactta ttccactaat gacgatttta
      481 acgattttag ccgtaggaaa cattttccga gctgattttg gcctgtttta taacattcct
      541 aggaattccg gtgcattata tgatgtgact caagttttgg atacgtatat ctacaatggc
      601 ctgacatcca caggagattt tggtatgacg gctgctgcag gactttacca atcagttgtc
      661 ggctttgtct tgttgatgat cacaaatacg atcgcccgac gttttgatag cgattctgca
      721 ttattctaag gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa gtccgctctt
      781 tcaataagac ggctaatttt ttctctaata taatgattgc actctttgct atttcctgta
      841 ttttgccatt tatatttgtt atcgccatct cttttaccga tgagtccata ttgtcgactc
      901 acgggtacag tttttggcca gaaaagttca gtatatttgg ttatacgtac ttgttcgacc
      961 agatgcagga taaaattttc caagcgcttt tcgtcactgt acttgttact gttttaggta
     1021 cactgatcaa tagcacggct acttctttat atgcttatgc gatttcacgg tcaaactttc
     1081 cattccgtcg tttttttacc gttttttgtt tgattacgat gttgttctca ccagggatgg
     1141 tggccaatta tcttgttatg acaaatcttc ttcagttaaa agatacgatt tgggcattga
     1201 tcttgccaat ggctgtcagt cccttcaata ttattgtgat gcgcacattc tttaaacgtt
     1261 cggtctctga ttcgattatt gaatcggcac ggatcgatgg agctagcgaa ctgcgaatat
     1321 ttgttcagat cgttttaccg ttagctgttc caggtattgc gacaatcagt ttatttgccg
     1381 cattgggtta ctggaatgac tggttcaatg ctttactata catccaagat gataagctag
     1441 ttccactaca gtatcttttg atgaaaatcc aaagtaatat tcaatatctg acacaaaatt
     1501 caggagcagg aagccaattg acaggcggat tagcctcagt tccaggagaa tctgccagaa
     1561 tggcgatcgt agtcatctcg acgttgccta ttgcgatcag ctatccattt ttccagaagc
     1621 attttgtcaa aggtctaaca atcggcggag taaaagaata aagaaacaaa aatataaatc
     1681 ataaaattag gaggaagaaa aatgaagtta tggaagaaac tagcctttac aggtgtttca
     1741 gcgttgttgt tgggaacatt agcagcatgc ggaggttctg gttctaaaga ccaggcagaa
     1801 gctagcaatt cggacacttt gcagatgtat caaatcggag ataaaccaga taatttcgat
     1861 caattgatgg aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa agtcaatatc
     1921 aattacattg gctggggcga ttacgaaaag aaaatgaacg tcatcatttc ttccggggaa
     1981 aattatgata tcgcctttgc gaacaactat gtttccaatg cccaaaaagg agcctttgct
     2041 gatttgactg aattagcgcc taagtacgct gaaaaagctt ataatgattt agatgaagca
     2101 tatattaaag gaaatctggt caatggaaaa ttatatgctt tcccagtcaa tggaaatgtc
     2161 tttgctcaac aagtacttac ttttaacaaa gctttactag ataaatacga tctttcaatt
     2221 gatggaatcc aatcttacgc tgatgcagaa aaagtcttga aggaattcca tgaaaaagaa
     2281 ccaaacacgg cagcttttgc cattggacaa ggcttcaaag tccaagggaa ctttgattat
     2341 cctttaggga atacgttacc atttgctatt gatctggatg gagatgattc gaaaatcatc
     2401 aaccaatacg ataatgaaac gtatatcgga ctgttacgta caatgcatag ctggtacaaa
     2461 caaggactca ttccaagtga cgctgcaacg agcaataagg attatccact agaaggaaat
     2521 acttggctaa tgcgtggaga aacacaagga ccgtatgatt atggcgatac aattttgaca
     2581 aatgcagcaa aacaagaact tgtctcaaaa gcaattactg ttccactaaa atctacagct
     2641 caagcacaaa tggcaaattt tgtcgtatca aatacttcaa aaaataaaga aaaatcagta
     2701 gaacttctag ggctgcttaa cagtgatcca gaattattga atgggcttgt ttgggggatc
     2761 gagggagaag catgggaaaa agtcgatggt tcagatgata aaatcaaatt gctagatggt
     2821 tatcagccaa atacacatat gtctgcttgg aatacaggaa acaacaagat tctatataca
     2881 caagagtcta ttacggatga catgattgcc aaacgtgatc aatcgattgc tgatgcagaa
     2941 acttcaccga ttttaggatt cagcttcaac actgacagtg tcaaaacaga attaagtaat
     3001 atttctaatg tcatgaatca atatttagat ggattgaata cagggactgt tgatccagat
     3061 gaaacattac cgaaattgaa agacgctttg gacaaggctg gctatgataa ggtattgaaa
     3121 gaaatgcaaa aacaatatga tgagttccga caagaataat aatgaataaa gacaaagggg
     3181 ttgtgacaaa agacgtgtca caacctcttg ttccgaataa acggtgttca aaagccagtt
     3241 tctcggtatt aagctaaata tatgtaaaaa tgtggaaagc catattcaca aaatttttct
     3301 tactactcga aactaagcgg ctatttcaca aactcttggt aaacggtgtt caatcagcta
     3361 accctcggta ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc gtttgtttct
     3421 ctaataatca ggtcaaggtg ctgattgccc aacctttttt tgtataatga gaaagtataa
     3481 acttaatgtt tggaggagaa gatatgttag ggaaggtttt ggaagagtta tttatccgga
     3541 tctgggtcat tataaaacta accttgtatt tctggatata tacatttgcc ggcgggatca
     3601 tctttggatt aggtgcggca tggaaaacag tcaatgaact attttatttg tacggattcg
     3661 agtataaaga aatcacaata aaaagaggct ggaatatata taaaagaaat tttttaagag
     3721 gcaatctgct atttagttta tttttatctg gtactgcttt attgagttac aatttatatt
     3781 tatctgttca aatcaaaggg ctcatttttt tagctattga ttttatttta ctttttgcct
     3841 tgctctgcct atttgctact tatcaataca gtatgatttt agatagtgaa tatacgatct
     3901 ctgttcctaa tctgctaaag ctaagtttta tttcggtttt ttccagcttt tcgtcttttc
     3961 taaaaataat tattggtagc ggtataattt taggcgtgac ttggcaattc aaaggattga
     4021 tcctgtttgg tgtcattggt cttttgacgg tttggaatgg tacgatgaca acacattggc
     4081 gtgaagaatt agataaacag ttggaaagtt atgaataagt ttcggattct attttcaaaa
     4141 ggaaccttac taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg tgtaacgatg
     4201 actgctaccg ttatttttag tatccatact tgggaacaaa atcaaaaaca ggcagaaaac
     4261 atgacaagtg atgccgttca aagcacaagc agaatgctga atgataaaac aaccttgagt
     4321 aggatcatca aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt gacgacctac
     4381 ttaacaaaac ccatagatca gtatctgatg tatgtatacg aacagcaaaa cagtaccgat
     4441 gaattagttt ctttcccaaa tcaaatcaaa gatctctacg caaattatga agaactaagt
     4501 gcgatatata tcgtgttgaa tcaactaccg gagtattatg aatcttctag agaaaacaaa
     4561 ggtggggaaa taaaaaaggg gacaccaaaa ttactagacg ccttttatat caagttgcct
     4621 atcacacaag gaggagctga gtcaggaagt atttttattg gttttcaaaa gaaagaacta
     4681 gatatgattt tagaaaatct aacgtcattc aatggactct ctttatacat gatttctgga
     4741 acgacaaatc gactatacac attccatgat aataaaatag ctaaagaaac gtttgaaaga
     4801 caagaaatga tcatcacaga gagtctgaag aaaaattcac aattgccgat tgacgatctg
     4861 gaaaaaaaca attttgttca acaccaagaa ctgtctgatg attatcgtat attagctgta
     4921 ctagataaaa aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct gatcattggt
     4981 atcattattt tagacaatat actgcttatc tttctttata aaacattcaa acgatattca
     5041 cagcaagtat ctatcgtcat gcaggccatg gatcaaacag cagcagggaa tttagagacg
     5101 cggatcgaca taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg tatcaacgaa
     5161 atgttggata gcatcaatca gtttgtagaa gacatctaca aattagaaat caaacagcaa
     5221 gacgcacata tgcgggcgct tcaagcacag atcaatccac actttttata taatacatta
     5281 gaatatatcc gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga tgttgtgtat
     5341 gcgttttccg ctttgcttcg aaacaatacg aatcaagaga aaacgatcac tttaaaagaa
     5401 gaactggact tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc taatcgagta
     5461 gcctatcatt ttatgatcga tcctgatttg gaaaagatcg aagtacctaa atttgtgatc
     5521 cagccattag ttgaaaatta ttttaaacac ggtattgatt ttactcgatt cgataatgcg
     5581 ctaagtgtca aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa agataatgga
     5641 aaagggatga ctgaaaaacg gctaaaacag attgaagaaa aactgtctca tcctaaagtg
     5701 gaacttcacg gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc cagcttcggc
     5761 agcagttatt atatgtcgct tgagaataat gagacaggtg gactgaccgt ttctattaca
     5821 ttcaaggagg gatgagcgtg tatcaagtgt tattggtaga tgatgaatac atgattttga
     5881 atgggttaaa aaagataatt gattggcaaa gtttaggctt tcaaatagta gcaacagcag
     5941 agaatgccaa agaaggacta gctgtacttg aacagcgaca gattgattta gtagtaacgg
     6001 atgtgacaat gccagaaata aatggactgg aatttattga agcggctcaa aaagaacgtc
     6061 ataattttga atttatgatt ttatcaggat accaagaatt cgattatttg aaaggaggta
     6121 tgcaattagg cgccgtgaat tatttgatga aacccgtgaa taagttcgaa ttggttgaaa
     6181 gcctcaagaa aatcaaaaca cggttagatc agcaaaatga acaaaaaaat caacaagaaa
     6241 tctatcaaga actgttattc agtcaatggt tgaatgaaga attggatgaa ataagtgaag
     6301 aagaaataat aggacaaata agtgagaagc agcataggat agtattgatt caactggcaa
     6361 gaactcatga tactcttatc aatagatggt tgcaagaacg gcaacaaatc ttttactatc
     6421 aacgaaatta cggagaccgt gtattgatca cgttgttgtt ggaggcagat gaagttgatg
     6481 gcttctgttg ttttgttcaa gaaaactttc ctgatcagga atggctgatc agtatcgggg
     6541 aagagacaga ttctttggac aaaatccctg aaacattcca acaagcgaaa gacaatctac
     6601 agttgcatca attttatgga gataaagacg agcatgtttt ttacgcagta caagcagaaa
     6661 caaaagatca aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat aaacaattag
     6721 aagtcgctca aaaaatgatc actgattttt ttgaacagtt tcagttagca gccatgatgc
     6781 cggaagatat acgatattcc agttttttac tgtttatgga gattcaacgg gaattgattg
     6841 atctggaaga tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca aaaacggtgc
     6901 aagaacttca gcagctactt ctgtcattca tcaaaaagca tcagagacag aaaaagtaca
     6961 gtgacaatgt ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa ccacttacac
     7021 tcaaagaagt gtcagaaaac ctgcatttaa atgtcatgta tctaggacag ctgttcaaaa
     7081 aagaaacgaa aaaaagtttt tcagcttatt tgaaccattt acgtatggaa aaagcgaaac
     7141 aactgctttt gcacagtaat caaaacatca atgagatagc aagtgagatt ggctacaaca
     7201 acaccacgta tttctctaaa ttattcaaga aaatcgtcgg cagatcccct aaagagtacc
     7261 gagaaaacag tggagaatca taaaagaaaa agccaatgta atgaaataac ttataaaaaa
     7321 ttgtctagaa atctaatttt gggctgttct tattacttga ggcaaggcag ctttttcatg
     7381 acctctcgat aaacggagtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
     7441 aatgtggaaa gccattttca caaaattttt cttaatactc gaaactaaac agctttttca
     7501 cgacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaatggc
     7561 aaaatatgag gaactatttt gccatttgtc tctctaatac tcaggtcaga acgctgattt
     7621 cacaacctct caataaacgg tgttcaaaag cttgcatctg cggtaataag accaaccaaa
     7681 cgaaaaatat gaggatctat ttttgcttgg ctgttcttat tacttgatgc aggacagctt
     7741 tttcacaacc tcttgttaat tatagtttga taaggaaaaa ggcaaatctt ttgcttgata
     7801 tttcttaggg tgagggatga tccctaattg gtaagagatc gtgccgcctt tcagcaaatc
     7861 ttgatgggta aaatataatt tcgtatattc tttgtccatc cgtttgattt tatggataaa
     7921 ttgttgctgc ggccgattag gcgttgcttc aatcgtcaat tcctttccat ttgacaaaag
     7981 caaaacagcc ttgtcaatta atggcatacc gataacatat tcaccggttc caggcgtgac
     8041 aggataaaat cccagactat tgaagatata ccaagcggcc atacttccat tgtcctcatc
     8101 tccagggtaa cccattggag aatcgtcgaa ggtctgagtc atcaactgtt taagcagtgg
     8161 ctgtgccatt tcaggttgtc ctatataact gaacaagtaa ggatagtgaa aacttggctg
     8221 gtttgaaata gctaattgtc caaattctat cgcggccatc tcactcattt cgtggatctc
     8281 aaaaccatat cctcctgggt caaacgttgg gggctgattg cacagctcaa tgagtttttc
     8341 tttatatctc tcactgccac catattttcc gatcagtcct gaaaaatcgt gataaacagc
     8401 aaagctgctt tgccaagcac ttccttctgc ataatcttgt ccccaacgat tagctgaaaa
     8461 tggttctctg aacttgccat ctttatcttt tgaacgcata aatcctgact catagtcaaa
     8521 gatattttga tagttcaatg attgctgctg atatttcttc gtcatttcct cttcttctag
     8581 taccttggca acttggctaa tacaaaaatc gctataagca tagtctaatg tgtgattgac
     8641 tgattcgtgg tagtgattag gaacatagcc atatgtcaag taatcttctg ttccttggcg
     8701 tccataattt ggatgtttgc tttgaaccgt agcacctttt tccattgccg ttagaaattc
     8761 aggcatcaag tctcgtcgga tatcttttac agcagcatct gcaatgaccg catcgatcaa
     8821 tgtcccaggc ataagtccac gttcatcagg agacagccat tttggcaaat agccactttc
     8881 tctatagctg ttcaaaaaac cttccagcat ttcttcgtat ttttctacag caatcaaaga
     8941 ataaagagga taaaccgttt tatacgtatc ccaaaaccca ttatttgtat agagaacacc
     9001 aggttttata gatcgactag tcgtatcata gtggattttt tgcccactag gatcaatttc
     9061 ataaaatgtt tgtggaaata aaaacacccg atataaatta tgataaaacg tccgcaattg
     9121 ttgttgatca tggtgtgtga gctgtatacg atcaaaatag gactgccatt gttcttgact
     9181 gtgagcaagg taagtctctt tgctttggtt ttgttctagt tccaggttat aagctgcttg
     9241 ttccgagctg ataaaagatg tcccaaatcg gatagtttgt ttttttgtct gttcaaaata
     9301 caacgggatc gagtcgctat ttttgttgga aacagctaac tcatcaattg ttaaaggata
     9361 ttcaaatctc aatataaaat aaaaagaaaa atcaggatct tctgaaccag caaaatgtgt
     9421 gatttttcct tccactgtgt aaggatctat aacaaagagc tgatggcgtc ccggaaagga
     9481 taaaagtaaa ccgctgtcat ttttgctgta atcaatagat aagattccgc catacatgct
     9541 aggtatcaat gtagaacaga tgccataacg taattgacag atcgacagat gagtcgggca
     9601 aaatatactt tcttctgggc ggtaagaact ttgagcatgg aaaagggtgt tctcctgtag
     9661 ttttccattg atcggcgtaa acagcatatg actgaaatca cccatccaag gacttggttg
     9721 atgggtaaga cggtagcctt gaaaaacgcg gtcatcagga tgaaaccacc agctgccgtt
     9781 ttgatccgta gtttgcggtg caaaataatt catcccaaaa ggaacgccag tataaggctg
     9841 gcagttccca ttagagaaat ttggctgatt cgctgttgca tgtctagtat ctatttgacc
     9901 gatattcatt ttatttctcc tttgttgatt gtcttttgat gaatgaaact ggaaatttac
     9961 tatcggatgg tcgatctttt gtctctaacc aagtgaccaa agcatctccc gcaagcttac
    10021 ctaactgttc aaaatcttgt gcgatggttg tcagtggcgg gtcaacaaga gctgctgctt
    10081 ggatatcatc aaatccgata attgaaaaat cttcagggat atgataacca tgctgtctca
    10141 agagattcat ggtctggata gcgacaacat cgttttcaca aacaaaagca gttacttcat
    10201 acttagtgat ataagaaagc aattcatcta atgtcgcgct tttgtcatcc agtgaagtgt
    10261 ggaaagtcaa accagctttg tccaatgctt caaggtaacc aaggtaacgc tgtcgaacgg
    10321 attgtggatg agtctgcgta ccaaacaaat aagcgatttt tgaatgtccc tgttcgatca
    10381 aataagaggc agcaagcgca ccgccttgga aattatcgga aagaacggta ggaaaaggta
    10441 actcatagat ttttttatct aatatgacga cagggaattc tttcaatgaa agctcaaaaa
    10501 gtaagtcaag atgttgttct gtatcaagag cataataaat caatccgtca aattcattga
    10561 ttagctcttt tgctgtttta ttttgtagaa agtcaagcgt cgtcatcatg acatcaatct
    10621 gtttatcttg catcacaggg ttcaatccct tgctaaaatc accaacagac aaatcagcga
    10681 catatggaag aagaaacaga atccgtcgtg tttttgtagg agaagcaaga atctgttccg
    10741 gagaagcgga tttaacgaaa ctgcctttcc cgcgaacgcg ataaataagt cctaattgct
    10801 ctaattcagt caatgcacgt ttggaagtga ttctgcttac tttataggta cgtgaaagtt
    10861 ctttttcagt tggtagttgg ctgtctacag gtaattcacc agttaaaata gccgtttgca
    10921 aatcattaaa tattgtctgg tacaatggtt gattcatata aacacctctt aatgatatat
    10981 catattcaaa taataccata atggatgaaa aagtaaataa aaagatattt tttgaaagcg
    11041 tagacataat tggctgtatg aagtataatc tttaaagata tatcaaatag aaagaaggtt
    11101 ttattagtgg cttataaaga tataccaaaa tcggtgcaga aatttatgga agatatcaca
    11161 caaaaatgtg gggatatcca tgcggactgg gcaaagaatt ttacagcagc atttgccaat
    11221 actctgttga caacagtcaa aaagcaaaat gacggaacca cctttttatt gacgggagac
    11281 attccagcga tgtggttaag agactctact gcacaagttc gtccttattt agtgattgca
    11341 aaagaagatg aggaattagc tgacatgatc agcgggttag tcaaaaagca atttttttat
    11401 atcaatatcg atccatatgc gaatgccttc aacgaagaag ctaacggtgc tggccaccaa
    11461 acagatcata caaaaatgaa cgattggatc tgggaaagaa aatatgaaat tgattcttta
    11521 tgctacccaa tacagttggc ctatcttctt tacaaaaata caggaaaaac tgatcaattc
    11581 aatcaatctt ttattgaagg tatcaaaaaa atcttggacg tgttcaaaac agagcaagat
    11641 catgaacact ctccttatac atttgtacga gacacgacaa gaaaagaaga tacgctgatc
    11701 aatgacgggc gaggtgcaaa aacagctttt acaggaatga catggtctgg tttccgtcct
    11761 agcgatgata cttgccgtta tggatatctg gtaccatcca atatgtttgc ggtggtcgtg
    11821 ttaggttatg tggaagaaat ttttcaaacg atactcgatg ataaagaaat tgtacagcaa
    11881 acacagaaat tgaagaaaga gatccaagag gggattcaga atcacggacg aacggttaat
    11941 caaaaagggc aaacgattta tgcatatgaa gtagatggaa tgggaaacgc cacagtcatg
    12001 gatgacagta atgtgcccaa tctgatcgcc gcaccttact tgggatattg ttctgaacaa
    12061 gaggaagatt atctgcaaac aagaagaaca ttgttgagta aagaaaatcc atatttctat
    12121 gaaggaaagt acgcaaaagg aattggatcc tcccatacgc cagaaaaata cgtttggccg
    12181 attgcattag ctatggaagg aatgacaacg tcagacaaat ctgaaaaaga gcggatattg
    12241 aatttacttg tagcaactga cgcaggaaca caccttatgc acgaaggatt cgatgtgaac
    12301 aatcctgaaa attatacgag agaatggttt tcttgggcaa atatgatgtt ttgcgaatta
    12361 gtcatggatt actttgatat tagagtagaa aagtaggagg tatcgtaaat gaaaaagaaa
    12421 gtatatatca tttctcatag ccactgggat cgtgaatggt acatggctta cgaacagcat
    12481 cacatgcgac tgattgaatt gatagacgat ctactagagc tatttgaagt tgatccgtcc
    12541 ttcaacagtt ttcatttgga tgggcaaacg attattttag atgactatct tcaagtaagg
    12601 ccagaaaaaa gacaggcaat ccaacaagca atcaatgaag gaaaattgag aattggacca
    12661 ttttatatct tgcaagatga ttttcttatc agctcggaat cgaacgtgag aaatatgctg
    12721 attggcatgg aagaaagccg taaatgggga acccctgtca tgttaggcta ttttcccgat
    12781 acatttggaa atatgggaca gacgcctcaa ttaatgaaac aagctggtat ttcagcagca
    12841 gcgtttggcc gtggtgtaaa accaattggc tttgataatc aagttttgga agcagaaaac
    12901 tattcttccc aatactcaga gatgtggtgg aaaggacctg atcaaacagc aatctttggt
    12961 ctgctatttg ccaattggta tagcaacggg aatgagattc cagtagaaaa agaagcagct
    13021 cttgcctttt ggaagcaaaa attagcggat gcagaacaat atgcctctac gaaccactta
    13081 ttgatgatga atggtgttga tcatcaaccg gtacaaaaag atatatcaaa agcaatccat
    13141 ctcgcaaatg aactgtttcc agattacgag tttattcatt ctaattttac tgactatctg
    13201 gaagcagttc aaaaagatgt gccagaagac ttaggctcag tagaaggaga attgacaagt
    13261 caagaaacgg atggctggta tactttagcc aatacagcat ctgctcgggt ttacttaaaa
    13321 caatggaata ccaaggtcca gcgacagcta gaaaatatta cagaaccact tgcgacgatg
    13381 gcttatgaag ttagtggaaa ctatccccat gatcaattag attatgcttg gaagacatta
    13441 atgcaaaatc atcctcacga cagtatttgc ggttgttcag tcgattctgt ccatcgggaa
    13501 atgatacctc gttttgaaaa agcagatgaa gttggaaagt atcttgcaca agattcctta
    13561 gaacagctga ccgcagcaat cgatacgacc ggattcccaa aagacagctt tccatttgtc
    13621 attgtaaata ctgcaggaat ggataaaact ggtgaagcag aaataaccat cgaattagaa
    13681 agaaaaagat tcgctgaagg aatacctgaa caactatatc aagagttaga aaatttgcct
    13741 aaacgtaagt atcatgtaga aacaaaaagt ggggccacta taccagctat tttatctgaa
    13801 gaaactgtcc aatttggtta tgatttgccg aaagaccgtt tccgtgtgcc atatatggca
    13861 agaatgatca aagtgacttt accattagaa aacatgccag cattttcttg ggaaacattc
    13921 gcgcttgtag aaggggaagc gaaagcagaa gaaaaggaaa caatgattca tcagagtgga
    13981 cgaatcatcg aaaacggccc tctacatttg acgattgaaa aaaatggtac cattacaatg
    14041 gaagatcgca aaaacaaacg aaagttgaat aatctgcata tttttgaaga tatcggcgat
    14101 ataggaaatg agtatatctt taaacaacct ttctgtgatc agccaatcct atccagtaac
    14161 aaagaaaact ctgaagtaaa agtattagta gatacaccgg aaatagcaaa aataagtatt
    14221 ctacaggaaa tggaaatccc tgtttctgca gacgagcgtt tagaaaaaga acagcagatg
    14281 gttgtggaat ttcgttatcg aaaagcagaa cgatccaaag aaaaacgaat ccttcaaata
    14341 aaaacaatca tgacaatcag aaaagatagt aaaaaaatcg actttgaaac gactctcgac
    14401 aatcagatga aagatcatcg cttacgtgtc ttgtttccta caaagttaca tgtggaacat
    14461 cacgaagcag atagtatttt tgaagtggtg aagcgcccaa accatgtgtc taaaagttgg
    14521 gaaaacccca cgaatccgca acatcagcaa gcatttgtca atattcatga tgaagagtac
    14581 ggtgtaacag tcggaaactt tggtttgaat gaatatgaag taacagaaga cggacaaatc
    14641 gcagtgacgc tacttcgcag tgtgggagaa ctaggcgatt ggggatattt tccaacacca
    14701 gaagcacagt gtctaggtga gcatcggttc aattacagca ttgaactaca tggaccagaa
    14761 gaaaaattct ctacttattt gcatgcatac gcagcacaga ttcctttttc cactcaacaa
    14821 atcaaacatc atgaaggaac attgatttca aaacaacaat atttaacgat aaaaagtgaa
    14881 acgtttgcaa ttacagcttt gaaacgcagc aagtttagtg ataaagtagt agtaagaggt
    14941 ttcaatatgt ctagtcattt agaaaaattg gaaataacta aagacaatgg aaaaactgtc
    15001 atattgaatc tgcttgaaga accaacgaag caagcggttg tcccaataat ccaaccttat
    15061 gagattcgga caatcggatt cgaggaggaa aacgaatgta tggcggaata gaagcaggcg
    15121 gaacaaaatt tgtgtgtgcg gtttcagatg gtggagaaat tttaga
//
LOCUS       DASJUF010000060        14943 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJUF010000060 DASJUF010000000
VERSION     DASJUF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.060"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(510..1076)
                     /locus_tag="U4B62_001772"
     CDS             complement(510..1076)
                     /locus_tag="U4B62_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2141928.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="U4B62_001773"
     CDS             complement(1076..2032)
                     /locus_tag="U4B62_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN2141929.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="U4B62_001774"
     CDS             complement(2032..2688)
                     /locus_tag="U4B62_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2141930.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="U4B62_001775"
     CDS             3058..5076
                     /locus_tag="U4B62_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2141931.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /locus_tag="U4B62_001776"
     CDS             5503..7917
                     /locus_tag="U4B62_001776"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN2141932.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="U4B62_001777"
     CDS             8265..9818
                     /locus_tag="U4B62_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN2141933.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="U4B62_001778"
     CDS             complement(10199..11983)
                     /locus_tag="U4B62_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141934.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="U4B62_001779"
     CDS             complement(11998..13719)
                     /locus_tag="U4B62_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2141935.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="U4B62_001780"
     CDS             13819..14580
                     /locus_tag="U4B62_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2141936.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4861 a         2745 c         2956 g         4381 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    14761 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    14821 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    14881 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    14941 aca
//
LOCUS       DASJUF010000061        14540 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJUF010000061 DASJUF010000000
VERSION     DASJUF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.061"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..807
                     /locus_tag="U4B62_001781"
     CDS             <1..807
                     /locus_tag="U4B62_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN2141937.1"
                     /translation="RFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNRRL
                     PRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFFDT
                     FVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEIVT
                     AVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFLLW
                     QIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            804..1604
                     /locus_tag="U4B62_001782"
     CDS             804..1604
                     /locus_tag="U4B62_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN2141938.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1765..3033
                     /gene="rseP"
                     /locus_tag="U4B62_001783"
     CDS             1765..3033
                     /gene="rseP"
                     /locus_tag="U4B62_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN2141939.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3101..4810
                     /locus_tag="U4B62_001784"
     CDS             3101..4810
                     /locus_tag="U4B62_001784"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN2141940.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5022..9380
                     /locus_tag="U4B62_001785"
     CDS             5022..9380
                     /locus_tag="U4B62_001785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN2141941.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9525..9998
                     /gene="rimP"
                     /locus_tag="U4B62_001786"
     CDS             9525..9998
                     /gene="rimP"
                     /locus_tag="U4B62_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN2141942.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10021..11196
                     /gene="nusA"
                     /locus_tag="U4B62_001787"
     CDS             10021..11196
                     /gene="nusA"
                     /locus_tag="U4B62_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN2141943.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11218..11511
                     /locus_tag="U4B62_001788"
     CDS             11218..11511
                     /locus_tag="U4B62_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN2141944.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11508..11819
                     /locus_tag="U4B62_001789"
     CDS             11508..11819
                     /locus_tag="U4B62_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN2141945.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11832..14138
                     /gene="infB"
                     /locus_tag="U4B62_001790"
     CDS             11832..14138
                     /gene="infB"
                     /locus_tag="U4B62_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN2141946.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14165..14512
                     /gene="rbfA"
                     /locus_tag="U4B62_001791"
     CDS             14165..14512
                     /gene="rbfA"
                     /locus_tag="U4B62_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN2141947.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4955 a         2583 c         3236 g         3766 t
ORIGIN      
        1 cgtttttttc cgcaaaaaaa caaatatatt caagaaaaaa gcgaatttca atttaatacc
       61 gatggaccaa ttcctcagca cattgccatc atcatggacg gcaatggtcg ttgggcgcaa
      121 aaccgtcgtt tgccacgagt tgctggccat aaagaaggca tggaaacagt aaagaaagtc
      181 acaaaaaaag catcaaggct tggagtgaaa gtgttgacgc tttatgcctt ttcgactgaa
      241 aactggaagc gaccaaaaga tgaagtaagt tttctcatgc aactacctgt cgacttcttt
      301 gatacgttcg taccagaatt gattaaagaa aatgtcaaag ttcatgtcat gggttatgaa
      361 aacgtattgc cagaacatac gcaagatgct gtaagacgtg caattgagca aacaaaaaac
      421 aatactggaa tggtattgaa ttttgctttg aattatggaa gtagagcaga gatcgtgact
      481 gctgtccaag aaattgccga agaagtagca aaaggagaaa tacacgcaga agaaatcgat
      541 gatgaattga ttgctaagca tttaatgaca ggtttcttgc ctaaagagtt acaagatcct
      601 gagctgatga tccgtacaag tggagaagag cgaattagta attttctgtt atggcagatc
      661 gcatatagtg agctgtattt tacaaaagcg ctttggcctg attttgatgg tgcgcatctt
      721 gaagaagcaa tcgcctctta tcaaaatcga gaccgtcgat tcggcggtgt aaaaaaaaca
      781 gaagatgaga aaggggatca gtcatgaaac aacgtgtgat cacagcagct gtggcgctag
      841 tcttgtttat tccgattata tggatcggag gcatagcggt tgagcttaca gcagcaatct
      901 tagcggtagt aggagtttac gagctgttcc ggatgaaagg attgacactc ttaagttttg
      961 aaggagtttt atccgccgtt ggtgcagtgt tacttgtttt gccaaaagaa agatggtttt
     1021 tctttcttcc agagaatgta agtacattta tgttatttta tcttatagtt atgattcttt
     1081 taggaacatc agttatttct aaaaatacgt atacgattga tgaagcaggt tttcctgtgc
     1141 ttgtcagctt gtatgtggga ataggtttcc agaattttgt caatgcacga acagaaagct
     1201 tggctgtttt gatatttggt ctatttattg tttgggcaac tgatatcgga gcatatatgg
     1261 ttggtcgacg ttatggtcag cataagttaa gtcctgatat ttcgcccaac aaaacgattg
     1321 aaggtgcgct cggtggtatc ggtagtgctg ttgtagtcgc atttctttat ttattgctat
     1381 acccagcaaa agaactattc aatcatggta tgtttgtcat gcttctgatg acgattattt
     1441 tttcagcagt cggacaattt ggtgatttgg tcgaatctgc aatcaaacgt cattatgatg
     1501 tgaaagactc gggaaatatc ttaccaggcc atggtggaat cttagaccgt ttcgacagtt
     1561 tgctgtttgt atttcctatc atgcatttat ttggcattat ttagtaagta tacctgcgtt
     1621 tctttgtgaa tttcataaaa gacgcaggtt tttatttttc attatattga tactattggt
     1681 gtctttggta tactatggta aaattatagt gttttatcgt tgttttataa aaaacacaaa
     1741 aaacaagtaa aggagaaaaa agctatgaaa acgattctga catttatcat cgtttttggt
     1801 atattagtga ttgttcatga gtttggtcat ttcttctttg cgaaacgatc aggaatcctc
     1861 gtccgcgaat ttgctatcgg tatgggtccg aaaatttatg ggcatcaagc aaaagacggg
     1921 acaacatata cgcttcgcct attacctatt ggcggttatg ttcgaatggc aggcaatggg
     1981 gatgatgaaa cagaaatggc accaggaatg cctctttctc ttttactaaa ttctgacggc
     2041 atcgtagaga agataaattt aagtaagaaa atccaattga caaatgctat cccgatggaa
     2101 ttgagccgtt atgatttaga agatgagctg actatcactg gttatgtgaa tggagacgaa
     2161 acagaagtag taacctaccc tgttgatcac gatgcgacga ttattgaaaa cgacggaaca
     2221 gaaatcagaa ttgcgccaaa agacgtacag ttccaatcgg ctaaattgtg gcagcgtatg
     2281 ctgaccaact ttgctggacc catgaataac tttattttgg caatcgtgtt attcatcatt
     2341 ctggcattta tgcaaggtgg tgttcaagtg acgaacacta atcgcgtagg agagatcatg
     2401 cctaatggag ctgcggcaga agcaggctta aaagaaaatg atgaggtagt cagtgtggac
     2461 ggaaaagaaa tccattcatg gaatgatctg acaacagtca ttacgaaaaa tccaggtaaa
     2521 acattagact ttaagatcga acgagaaggt caggtgcaat ctgtcgacgt gacaccgaaa
     2581 tctgtagagt caaatggcga aaaagttggc caacttggta taaaagcccc aatgaataca
     2641 ggttttatgg ataagatcat tggagggacg cgtcaagctt tcagcggttc attggaaatt
     2701 ttcaaagcct taggatcact atttacaggt ttcagtttag ataaattagg cggacctgtc
     2761 atgatgtacc aattgtcttc agaagcagca aatcaaggtg taacaactgt tattagtttg
     2821 atggcgcttc tttcaatgaa tctcggaatc gtcaatctgc ttccgattcc tgccttagat
     2881 ggcgggaaat tagtcttgaa tatttttgaa gggattcgcg ggaaaccatt aagtcaagaa
     2941 aaagaaggaa tattgacatt agctggcttt ggatttttaa tgttgttgat ggtgctagtt
     3001 acatggaacg atattcaacg attctttttc tagaaaaagt tgacaaatag ggtataataa
     3061 caaacgataa ctattttgag gaaataaagg agtatataga atgagacaat caaaaatgtt
     3121 gattccaaca ttacgcgaag ttccaaatga tgcagaagta ttaagccatc aaatcttgct
     3181 aagagctgga tatatccgcc aagtatcagc gggtatttat tcatatctgc cattagcaaa
     3241 tcgtgtattg gaaaaactga aacgcattat gcgggaagaa tttgaaaaaa tcggagcggt
     3301 agaaatgctg atgcctgcca tcttgccagc agaactttgg gaagaatccg ggcgttacga
     3361 aacatatgga ccaaacttgt atcgtttcaa agaccgcaat gatcgaaaaa tgatccttgg
     3421 accaactcac gaagaaactt ttactgattt gatgcgtaat gaaatcaatt catataagaa
     3481 attaccttta aatctttatc aaatccaacc taaatatcgt gacgaaaaac gacctcgctt
     3541 cggtttgctt cgcggaagag agtttatcat gaaagatgca tattctttcc atgcaacgga
     3601 agaaagtttg gacgagacat ataaagatta cgaaaaagcc tataccgaag tattcaagcg
     3661 ttgtggctta gaatttcgct cgatcatcgg agacggcggc gcaatgggcg ggaaagactc
     3721 aaaggaattc atggcgatct cagaaatcgg ggaggatacg atctgttatt cgactgaaag
     3781 tgattatgct gcaaacttgg agatggctac aagctactat gtatcaaaaa aatcgcatga
     3841 aacacagctt gaattggaga aagtagctac acctaacgta aaatcaattg acgaagtagc
     3901 agatttcttt tctgttgaac ctcaaaaaat catcaagtct gtattgttca ttgcggatga
     3961 acaacctgtc cttgttttgg tacgtggaga ccatgaagtc aatgacgtaa aactgaaaaa
     4021 ctttttatct gctgactttt tagaagaagc aacagaagaa gaagcacaaa aatatctagg
     4081 tgctgagttt ggttctgtcg gaccagtcgg tgtttcagat gacgtaaaag tatatgcaga
     4141 ccgttatgtc caagatttag cgaatgccat tactggagcg aacgaaacag gattccacta
     4201 taaaaatgtg aatccagaac gcgatttcac tgtgctttct tatgaagatc ttcgttttgt
     4261 acaagaagga gatccttcac cagacaacaa cggtgttctt gcctttactc gaggaatcga
     4321 aatcggacat atattcaaaa tcggcactcg ttacagtgaa tctatgggag caactgtact
     4381 agacgaaaat ggacgagaaa aatttgtgat tatgggctgt tatggtatcg gtgtaagtcg
     4441 tttattatca gcaatcgtag aacaacattc agatgaacaa ggaatcaatt ggccaaaagg
     4501 aattgcgcca tttgatctgc atatcgtcca aatgaatcta aaagacgaat accaaacaag
     4561 cctgacgaat gaactagaag aagcgatgac aaaagctggc tatgaagtac tggttgatga
     4621 cagaaatgaa cgcgcaggag tgaaattcgc ggattctgat ttgattggct gtccaatcag
     4681 aatcactgtc ggtaaaaaag cagtggatgg aatcgtagaa gtgaaaatca agaaaactgg
     4741 cgaaatggtt gaagtacgta aagaagaatt ggcgaatact ttaccaatcc tcttgaacca
     4801 aggcgaataa taagcgttaa aacacagaaa ataaaaatag ctgaatcagc tgtttttgac
     4861 aatctgatga ttcgctttta aaattggaac tgcgatttga atcgcagttc caatttttta
     4921 atgtaaaact cagaaaaaac gagtatacta aaaggtactg aaaaagaaga ggataagatg
     4981 cgatcaattg cattgtttct catcagaaag ggcgtatatc tttgtcagaa aaacgagatt
     5041 tatttgaaaa actgttggaa caaatccaac tagaggaagc agaaaaaaat catccactgc
     5101 ttactgctgg tgaaatggat agtgtagtgg tccatcgtaa atcgcgttta tgggaattta
     5161 cgctacattt ttccaaaatt ttgcctatta tgctatatcg gtcattgact cagcatctga
     5221 ctatcgcttt taaagatatt gcacaagtca aactgaatat cttagcagat gaccagacat
     5281 tcgatgaaca gctcttgcaa gattactggg cccaagcttt ggaaaatcag caatgcgata
     5341 caccactagc acagcaagta ttgaaaacgc aggtacctgt gataaaagat cgtaaagtca
     5401 tcttgccaat cgacagtaca ggagctattt cttatttgaa gcaacaatac ttgccattag
     5461 tagaagagtt gttcgtcagt tacggattcc caaaatttcg tatcgaacca gaagtggatg
     5521 agcagcaagc ggaacgagta ttgaaacttt ttgaagaacg caaacaagaa caggcagaag
     5581 cctttatgaa gcaagcagct gaaagtctag ttgtccacga acaaaagaaa aaggaaagaa
     5641 aagagcaatc gccagctctt gaaggaccca tccacatcca gctaggacga aatattccag
     5701 ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg
     5761 aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt
     5821 tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag
     5881 atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc
     5941 aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac
     6001 atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata
     6061 gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggga
     6121 aatggggaca ccgagctatc gccattacag accacggtgg tgcgcaagcc tttccggaag
     6181 cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg
     6241 tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat
     6301 atgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc
     6361 tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc
     6421 caggacatcc tttgtcacga acaacggtag atctgactgg tatcacggat ggaatggttc
     6481 gaggatcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa
     6541 ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt
     6601 atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct agatatctgt
     6661 atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaaatttggt gtcagcttgg
     6721 aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg
     6781 tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg
     6841 gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc
     6901 aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac
     6961 gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct
     7021 cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa
     7081 gaaaccgagc aaaattctac gattatattg aagttatgcc aaaagcggtc tatgcgcctt
     7141 tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg
     7201 tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga
     7261 acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt
     7321 tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat
     7381 ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg
     7441 cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag
     7501 gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc
     7561 cgttacctga gatcattcaa aaacgattaa aaaaagagtt gaattctatt aatggtaatg
     7621 gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct
     7681 atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca
     7741 ctgaagttaa tccgttagcc cctcattatt attgtccaga gtgccaatat tccgagtttt
     7801 ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg
     7861 gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg
     7921 gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata
     7981 actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag
     8041 tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt
     8101 ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg
     8161 gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc
     8221 cgatccaata tccagcagac gatcaaaatt cggaatggaa aacgactcat tttgattttc
     8281 actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta
     8341 ttcggatgct tcaagattta tcagggatcg atccacaaac gatccctacg gatgacccgg
     8401 aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt
     8461 ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga gggatgctag
     8521 aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta
     8581 cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg
     8641 aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg
     8701 ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg
     8761 aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg
     8821 attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga
     8881 tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact
     8941 tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga
     9001 aacaagcaat gaaagaaatc acggacaaag gattagatgc ttccgtaaaa gaaaagaatc
     9061 aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga
     9121 ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac
     9181 catttagagc tgtcccaagt ttgggaacca acgtggcaaa acaaatcgtt gaagcaagaa
     9241 aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat
     9301 tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat
     9361 cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta
     9421 tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct
     9481 ttttaatgga attacgcact taatttttta aggaggcgaa acttttgagt agcgtcgttg
     9541 aaacagtcac agaaatggtg acaccgattt tagatgaaca aaattttgaa cttgtcgaag
     9601 tcgaatttgt aaaagaagga aaaagctggt tcttacgagt atttattgac aaagaaggcg
     9661 gtatcgatat cgaagaatgc gcctttgtca gtgagaaact gagtgaaaaa cttgatgcag
     9721 cagatccaga tccgattcct caagcttatt tcctggaggt atcctccccg ggagcagagc
     9781 ggccattgaa aaaagaaagc gactatgaaa aggcacttgg tgattatgtc catgtctcct
     9841 tataccaacc agtagatggc gaaaaacaat atgaaggatt tcttcaatca tttgatgctg
     9901 aacagcttac attgaaaata cgcataaaaa cacgagaaaa agaattgact tttgaccgaa
     9961 aaaatattgc aaaagctcgc ttagctatcc aattttaatc acggagagga atcagaaaaa
    10021 atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa
    10081 gaaatcgtga tcgatgcttt agaagcagca ttggtttctg catacaaacg tcactatggc
    10141 caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg
    10201 gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca
    10261 cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa
    10321 gattttgggc ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa
    10381 gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga
    10441 atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt
    10501 ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt
    10561 tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat
    10621 ccagacctat tgcgccgctt atttgagcaa gaagtaccag aagtctatga tggaatcgtt
    10681 gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat
    10741 gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca
    10801 atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct
    10861 gtattcattg caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca
    10921 aatccaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa
    10981 cgcggacaaa acgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca
    11041 gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg
    11101 catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc
    11161 aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg
    11221 aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa
    11281 gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa
    11341 atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat
    11401 aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt
    11461 cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta
    11521 atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg
    11581 gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa
    11641 ccgatgcaag cgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc
    11701 cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg
    11761 tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg
    11821 aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta
    11881 gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg
    11941 cgattactag caatgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac
    12001 aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta
    12061 aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg
    12121 gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg
    12181 gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta
    12241 acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc
    12301 gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac
    12361 cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag
    12421 atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg
    12481 gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg
    12541 actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct
    12601 tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagtc gtaacgatta
    12661 tgggacacgt agaccatggg aaaacaactt tattggatac attacgccat tcacgtgtaa
    12721 caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg
    12781 gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac
    12841 gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc
    12901 cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg
    12961 taaataaaat tgataaacca ggtgcaaacc ctcaacatgt tatgcaagaa ttaagtgagt
    13021 atgagttaat tcctgaagca tggggtggag aaacgatctt tgttgagatc tcagcgaaat
    13081 tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt
    13141 taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag
    13201 gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcatgtt ggtgacccaa
    13261 tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg
    13321 ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag
    13381 caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac
    13441 gcgcgaaacg tgcattatta gaaaaccgtg ctgccacaag ccgtgtgact ttggataact
    13501 tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg
    13561 tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc
    13621 gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag
    13681 cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc
    13741 aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag
    13801 aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc
    13861 aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg
    13921 taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa
    13981 tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa
    14041 tgggttatga atgtggtgca atgattgaaa aattcaatga catcaaagta gatgacgtaa
    14101 ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat
    14161 cattatggct aactaccgtg accgccgagt cggtcaagaa atcttaaaag aagtcaatga
    14221 tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa
    14281 agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc
    14341 agataagaaa acagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt
    14401 agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt
    14461 tgtctatggc aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaac
    14521 tcccgcaaat cctgtgaaat
//
LOCUS       DASJUF010000062        14308 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJUF010000062 DASJUF010000000
VERSION     DASJUF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.062"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(220..3894)
                     /gene="rpoC"
                     /locus_tag="U4B62_001792"
     CDS             complement(220..3894)
                     /gene="rpoC"
                     /locus_tag="U4B62_001792"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN2141948.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3952..7569)
                     /gene="rpoB"
                     /locus_tag="U4B62_001793"
     CDS             complement(3952..7569)
                     /gene="rpoB"
                     /locus_tag="U4B62_001793"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2141949.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8297..9265
                     /locus_tag="U4B62_001794"
     CDS             8297..9265
                     /locus_tag="U4B62_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2141950.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9283..10188
                     /locus_tag="U4B62_001795"
     CDS             9283..10188
                     /locus_tag="U4B62_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2141951.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10185..10991
                     /locus_tag="U4B62_001796"
     CDS             10185..10991
                     /locus_tag="U4B62_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2141952.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11127..12116
                     /locus_tag="U4B62_001797"
     CDS             11127..12116
                     /locus_tag="U4B62_001797"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN2141953.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12433..12957)
                     /locus_tag="U4B62_001798"
     CDS             complement(12433..12957)
                     /locus_tag="U4B62_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN2141954.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13244..>14308
                     /gene="scm"
                     /locus_tag="U4B62_001799"
     CDS             13244..>14308
                     /gene="scm"
                     /locus_tag="U4B62_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN2141955.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4049 a         3080 c         2814 g         4365 t
ORIGIN      
        1 tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac ttttgttgat
       61 tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc gatgttgtta
      121 agatcctttc ccacttttct tctagttaga caaattattt tagacaaacc aaaaaagagg
      181 ttgtgacaat aatttgtcac aacctctttt ttaaaatatc tattttgttt gattgttcat
      241 tgcgtcttct gcagccattt gcgcttcaat atctgaaata ctgtagacat tttcgcttgc
      301 tacaccgact tcttttggtt ccatgtcacg ataacgttgc ataccagtac cagctgggat
      361 gatcttacct ataataacat tttctttcaa accaagaaga ggatctttct taccgcggat
      421 cgcagcatcc gtcaagacac gagttgtttc ttggaatgat gcagcagata agaaactatt
      481 tgtttctaat gatgcttttg tgatacctag taagacaggt cgagaagttg caggaacgcc
      541 tcctgagact agcgtattgt agttttgatc tttaaagtct gcgatgtcta ataatgtacc
      601 tggtaagata tctgtatcac ctggatccat tacacgaact ttacgcagca tttgacgaac
      661 cattacttcg atatgcttgt cgccgatttc taccccttgc atacggtaaa cacgttgtac
      721 ttcacgaagc aagtagtttt caacagataa tacatcgcga acttgcaaca attgttttgg
      781 atcgattgat ccttctgtca atggtgcacc acggtggatc atatcgcctt ctgctacttt
      841 catacgtgct gtataaggaa cagtatacgt acgtgtatct gtttttcctt tgattgttac
      901 ttctttggta cgtgttgcag gatcttctga gatatcgatg acatcccctg ttacttctgt
      961 gatcactgct tgccctttcg gattacgtgc ttcaaagatt tcttggacac gaggaagacc
     1021 ttgagtgata tcgtctccgg caaccccacc tgtatggaat gtacgcatcg tcaactgagt
     1081 accaggttca ccgattgatt gagcggcgat cgtaccaact gcttctccga cttcaacgtc
     1141 agagccagtt gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt ttgtattaca
     1201 tgtgaagaca gaacggattg tcactgtttc gacacctgca tcaacgattt gttttgcgat
     1261 gtcttcggaa atcaattggt ttcctgcaat gatcaattca tttgtttcag gatgacgtac
     1321 ttctttacgg gtgtaacgtc ccactaaacg atcttctaat gattcgatga tttcatttcc
     1381 ctcgcggatg gcttggattt caagacctcg gtcagtaccg caatcttctt cacggatgat
     1441 tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg cagtcttcaa
     1501 ggctgtatcg gtcatccctt tacgagcccc gtgagttgag ataaacattt ccaagacgga
     1561 tagtccttca cggaagttcg aaatgattgg caattccatg attcgaccat taggagcggc
     1621 catcaaacca cgcattccgg caagctgtgt aaagttggaa atgttaccac gagctccaga
     1681 atcagacatc atgaagattg gatttttcgc ttccaaacct tccatcagtt tttgttggat
     1741 gctgtctttt gcagcattcc aaacagcaat gacacgttca taacgttcgt catctgtgat
     1801 cagaccacga cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat tttcaatgat
     1861 ttcttgtttt tcattcagta cactgatgtc agcgatccct actgtgatac cggcatgtgt
     1921 cgaatgtttg taccctaagt ctttcatacg gtcaagcatc ttagaagttt ctgttacttt
     1981 gaatcgtttg aagacttctg cgatgatgtt tcctaagttt ttcttcttga acggtaatac
     2041 taattcttgc tctttgatat gtgcaggaat atcagtacca gcttcaacaa agtatttgtc
     2101 cggtgtttgg acggtcaagt tgaagtctgt tggttcattc agataaggga attcaggagg
     2161 catgatctcg ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt ctttttgcca
     2221 ttcagtaaat ggtttttcac ctaatgagtt agggttcaca ccgatacgtg aatgtaaatg
     2281 aacatatccg ttgcgccatg cgataacggc ttcgttttga tctctgaaga ccattccttc
     2341 gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta cctagaacca tatcttggga
     2401 aggagtaaca actggtttac catctttagg gttcaagatg ttttgagcag ctaacatcaa
     2461 catacgtgct tctgcttgtg cttcttcatt caatggaacg tggacagcca tttggtctcc
     2521 atcgaaatcg gcattataag cttcacatac aagtggatgc aaacggattg cgcgtccttg
     2581 tactaaaact ggttcgaatg cttggatacc aagtcggtga agcgtaggtg cccggttcag
     2641 caatactggg tgttccttga ttacgtcttc taaaacatcc catacttcgt cttcttgacg
     2701 ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa caagctcacg
     2761 catcacgaat ggtttgaata attcaatggc catttctttt ggaagtccac attggtacat
     2821 cttcaagaaa ggtccaacaa cgataacgga acgacctgag tagtcgacac gtttacctag
     2881 caagttttga cggaaacgac cttgtttccc tttcaacata tgagaaagag atttcaatgg
     2941 gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca atgcatctac
     3001 tgcttcttgc agcatacgtt tttcattttg aacgatgatt ccaggtgcat tcaagtctaa
     3061 caaacgtttc aaacggttat tacggttgat tacacgacgg taaagatcat tcaagtcact
     3121 tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt aaatctggtg gaataacagg
     3181 gataacgtcc ataaccatcc agcttggttg atttcctgaa gcgcggaatg cttctaaaat
     3241 atccaaacgg cggattgcac gtgtacgttt ttgtccttgt gcagatttca attcttcttt
     3301 caattcggct gcttctgcat ctagatcaac attatcaagt aattgtttaa tcgcttctgc
     3361 acccatagca gcttggaatt cttgtccata ttgttcacgt ttttcgcgat attcgcgttc
     3421 tgtcaacaat tgttttttct ctaaattcgt atttcctggt tcgatgacta catatgaagc
     3481 aaaataaatg atttcttcca aagcgcgtgg gctcatgtct aatacaagac ccatacgtga
     3541 aggaatccct ttaaagtacc agatatgtga aacaggagct gctaattcaa tatgtcccat
     3601 gcgttcacgg cgaactttcg aacgagtaac ttccactccg catcgatcac aaacgattcc
     3661 tttataacgg atacgtttgt attttccgca ggcacattcc cagtcttttg taggaccaaa
     3721 aatccgctca cagaacaacc cttcgcgttc aggttttaag gtacgatagt taatcgtctc
     3781 aggttttttt acttcaccgt aagaccagct tctgattttt tcgggagaag ccaaacctat
     3841 ttgcatactt tcgaatttat ttacatcgat caaaaggggt tccctccatt tcatttaatg
     3901 gcaaaccgca agacggctga tcttcgaccg ccttgcagct catagaccgg attagtctaa
     3961 attatcttct gcggtttcaa agttttggat gacatcgtct ttttcatctt ccacttgttc
     4021 agcagctttc ttttctaatt ctttggcagt ttgctgttcg gcaaattttg tcaaagcatc
     4081 aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct ctgtttcttc
     4141 gatatccaag acacgcatgt ccagacctag tgattgcaat tctttcacca atacgcggaa
     4201 tgattccggt acacctggtt ttggaattgg ttcacccttg acgattgctt cgtatgtttt
     4261 cacacgtccg actacgtcat ctgatttgta tgtcaagatt tcttgtaaag tataagccgc
     4321 accgtaagct tccagtgccc atacttccat ttcaccaaaa cgttgtccac caaattgagc
     4381 tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac gtgcatgcaa
     4441 tttgtcatcg accatgtgag caagtttgat catgtacatc acaccgacag acacacgtcc
     4501 atcaaatggt tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc tggccatacc
     4561 ggcttcacga actgtttccc atacgtcttc atcacttgca ccatcaaata ctggcgttgc
     4621 tacgtggata cctaactgac gtgcagccat acccaagtgt aattccaata cttgcccgat
     4681 gttcatacgt gaaggcaccc ctaaaggatt cagcatgata tcgattggtg ttccatcagg
     4741 taggaatggc atatcttctt ccggcataat acgggataca acccctttat ttccgtgacg
     4801 accggccatt ttatcccctt catggatttt acgtttttga acgatataaa cacgaactaa
     4861 catgtttacg cctggtgaca attcatctcc agcttcacga gtaaagattt tcacatcgtg
     4921 aacgataccg ccgccgccat gtggtacacg taatgatgta tcgcggactt cacgagcttt
     4981 ttcgccaaag atcgcatgca atagacgttc ttctgcagat aattctgtta ccccttttgg
     5041 cgtgactttc cctacaagca aatcgccatc ttggacttct gcaccaatac ggatgatacc
     5101 catttcgtct aaatctttta atgcgtcttc cccgacatta gggatttcgc gtgtgatttc
     5161 ttcaggtcct aattttgtat cgcgggcttc tgattcatat tcttcaatat ggatagaagt
     5221 gtatacgtca tcttttacca aacgacggct catgatgatc gcatcttcat agttgtaccc
     5281 ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc cttgttccat
     5341 agatggtccg tctgccagag tatccccttt ttcgactttc tcacccaaat gaacgattgg
     5401 acgttggttg tagcttgttc cggagtttga acgacggaat tttgttacat cgtatttatc
     5461 taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt caacgacacc
     5521 gtcatgttta cataacaaag cagctcctga gtcatgcgct gatttgtatt ccataccagt
     5581 accaacccat ggtgcttgag gattgatcaa cggcactgct tgacgctgca tgttggcacc
     5641 catcaaggca cggttggagt catcgttttc caagaaagga atacatgctg tcgcaacggc
     5701 aactacttgt ttaggtgata cgtccatgta gtctacttta tcaatagata cttctaagtt
     5761 ttcactttga gcacgagcca taacaacgtc ttgagcaaat gttccatctt cattcaatgg
     5821 gctgtttgct tgggctacga tgtaatggtc ttcgatatca gctgtcaagt agtcgatttg
     5881 gtcagtaaca cgacctgttt cacggtctac acgacgataa ggtgtttcga taaatccgaa
     5941 tttatttact ttcgcataac tggacaaact attgatcaac ccgatgtttg ggccttcagg
     6001 agtttcgatc ggacacatac ggccatagtg ggagtagtga acgtcacgaa cttcataacc
     6061 ggcgcggtca cgagtcaatc cgccaggtcc taaggctgaa agacgacgtt tgtgtgtcaa
     6121 ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac tgagaagaac caaagaattc
     6181 tttgatactt gccacaactg gacggatatt gatcaattgt tgaggtgtca atgtttcagt
     6241 atcttggatc gacattcttt cacgaaccac acgttccata cgtgctagcc cgatacggaa
     6301 ttggttttgt aaaagttcac cgactgaacg gatacgacga tttcctaagt ggtcgatatc
     6361 atcgacattg ccgatacctt ccatcaagtt caagaaatag ctcattgaag caatgatgtc
     6421 ggctggacga accgttttta cagctgcttc tggatagccg tttccgatca cgtttacttc
     6481 acgttcaggg tctcttggtg aaaatacttt gatgacttgg acagtcattg gatctgtgac
     6541 cacaccgtct tctgatggat agtaagtcac tgaatttagg ccattatcaa tgaaaggagc
     6601 tagtgtttcc atgacttggt gagtcagaac tgttcctttt tcaacgatga tctcgcctgt
     6661 ttctggatca actaatgttt cagctaatgt cagattcaat agacgtgttt tcaaatctaa
     6721 ttttttgttt actttgtaac gaccaacgtt tgctaagtcg taacgttttg gatcgaagaa
     6781 acgagcattc agcaagttac gtgagctgtc tgctgttttt ggttcgcctg gacgcaaacg
     6841 ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt tatgcaagtc
     6901 tttttcgact gtattgcgta atgtttcgct gtcgccgaag atttcaaaga tcgtatcatc
     6961 ggaaccaaat cctaacgcac gaactaatac ggttaaagga atcttacgtg tacggtcgat
     7021 acgtacataa gagatgtctt ttgcatcagt ttccatttct agccaagctc cacggttagg
     7081 gataacagta gaaccgaatc cttcttttcc gtttttgtcg acttttccat ggaaatagac
     7141 acctggtgaa cggactaact gagaaacgat tactcgctct gccccgttga tgatgaatgt
     7201 tccttgttct gtcattaatg ggaaatcacc gaagaatact tcttgtgctt tgatttcgcc
     7261 agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt gcagagtagt ttgcatcatg
     7321 cgcacgagct tctgctactg tatactttgg ttctttcaat tcataatcta caaattccaa
     7381 agatagattg ccattgaagt catcgattgg caaaatgtct tcaaacattt ctctaagtcc
     7441 ctcatctaag aaccattggt aagagtctgt ttgaatttcg atcaaatttg gtaattccaa
     7501 cacttcactg attcttgcga aacttctacg ttcgcgatgt tttccgtatt ttactacgtg
     7561 tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa agactacccg atcaattgtt
     7621 acaataatat ttcaaatatt atttttaggt aacaacagac attttttgcg gcttttaggc
     7681 aaaaaaaaac caaaaataga acattctctt gaaaccttac ttcgtgagct tctacttttg
     7741 tttccttata ttaaagccga aacggacaat atttcgtttc tgccttttct tcatcagata
     7801 gtttttgcaa cttcatatac tacacttatt ttctataaaa gtcaacactt cctggcgttt
     7861 cttctgcttt ttcgcaacat tttcttaatg ttttcaattt tatgttttca attatctgac
     7921 aattcttaat tgacagacct ttttatctcg gttatacttt agaaaattca ttttgtggag
     7981 gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat aacttttact
     8041 ttagctactt tagataggtt tgtattcatc atgatggata caaacgttaa acgacagttt
     8101 gtatctatga tggctaaaga actttgtatt gcagcaaaaa gccacgtaga caaaatgcta
     8161 agtggctgag cagtgaaaat acaaagtaaa agtatcaagc attctacttt ttcaatttat
     8221 tttccgactt ttcaatccat ttggacattt tgtctgaatg gattttttgt ttcccaaaat
     8281 taaggaggaa aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg ccctgctact
     8341 agctttagga ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg gacaaaaaac
     8401 aattggcgta ttgcaacttg tacaacataa ctcgttagat gctgcctata aaggattcaa
     8461 agaaggatta gcagaagaag ggtataaaga aggcgataat ctaacaatcg attatcaaaa
     8521 tgcccaaaac aatcaagaca acctgaaaag tatgagtgag aaactcgtca aagaagatcc
     8581 tgatttgcta cttggaatcg ccactccagc agcacaatct ttattaaatg aaacaaccac
     8641 tatcccgatg actgtcacag cggtcacaga cttggaacaa gcccatttag tcacatccaa
     8701 tgaaaaacct ggcggcaatg tgactggcac gacagatatg gtgccaatcg aaaaacaaat
     8761 cggcttactg ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt ataacaacgg
     8821 tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa aagcgggcgt
     8881 caaagtcaaa gtactgacag caaattccac aaatgatgtc caacaagtga cgaaaagtct
     8941 agctaaagat gtagatggaa tctatattcc tactgataac acgttcgcaa gtgcggcaag
     9001 tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg cagttgatca
     9061 agtcaaagac ggcggtctag caacagtagg aatcgactac gaaagcttgg gtaaacaaac
     9121 agggatcatg gcagcaaaaa tcttagacgg caagaatcca agtgaacttc ctgtcgaaac
     9181 agcgaatgat ttggaactgt atgtcaataa agacatggcc aaagcattag gcatcaatcc
     9241 agacagcatc aaagaagctg aataaaacac aggaggattc tcatgacaaa tttgctctct
     9301 cttttcttat catctgcatc gcaaggggtc ttatgggccc tacttgcgat tggtgtatac
     9361 gtcactttcc gtatcttgga cattgccgac ttgactgctg aaggaagctt tcctttaggt
     9421 gcagggatcg cggcagtaag tattacaaat ggctatcatc cattgacagc ttgtcttcta
     9481 ggctttttag ggggtgcggc agcagggctt gtttccggac tgcttcacac aaaattgaaa
     9541 attcccgccc ttcttgcagg gatcatcacc atgactggtt tatattctgt cacatcccgt
     9601 gtgatgggtg cgccgaacat ctctttattg ggtcagccaa cgatctttca ttgggtcgag
     9661 tcattcggca taacaaaaga caacgctgta ctgatcgtcg gtctgctttt tgctttgatc
     9721 gttgtatgtt tacttgtttt gttcttacgt acagaaacag gacttgctat tcgtgcaact
     9781 ggcgataatc tggcaatgag cgaagcaaac gggatcaaca cagataatat gaagatcatc
     9841 ggttatatga tttctaatgg atgcattgct ctttctggct ccttgcttgc gcaaaacaac
     9901 ggctttgctg atttaaattc tggtattgga acgatcgtaa ttggacttgc ttctattatc
     9961 atcgcagaag ttttgttacg taaccagcca ctatttctcc gtctcttgac tgtcgtttta
    10021 ggtgcggtca tttatcgttt tattttagcg ttagtcttcg aactgaatgt tgaaccaagt
    10081 gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc gcagatccag
    10141 caaaaattcc atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat gacgtcttaa
    10201 cattggaaaa tctccatcat acctttgaaa aaggaacgat caacgaaaat catgtcctga
    10261 aaggcattaa tctttcctta gatcccggcg acttcgtgac gatcatcggt ggaaatgggg
    10321 ctggtaaatc tactttgctg aacagcatcg ccggtacatt ttctgtcgat cagggtcaaa
    10381 ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg aaatccatca
    10441 gtcgcgtatt ccaagatcct aaactaggga cagccgttcg tttaacggta gaagaaaatc
    10501 ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc aaaccacaag
    10561 accgaacatt tttcaaagac cagctggttc ggctaaattt ggggttggaa aatcgtttat
    10621 cctcagaaat cggtttgctg tctggaggac aacgtcaagc tattacgtta ttgatggcca
    10681 cattacagcg tccagaactg atcttattag atgaacacac tgcagcgctt gaccctgcta
    10741 cttccaaaac ggtgatggaa ttgacagaaa agctgatcac tgagcagaat ctcactgctt
    10801 ttatggtcac tcatgacatg gaagacgcga tccgctatgg gagtcgactg atcatgctgc
    10861 atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc acagtgcccc
    10921 aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat caagtacttt
    10981 tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc ttcttttttc
    11041 tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa aactattttg
    11101 ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg acaacgaaag
    11161 acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa aagttggcac
    11221 agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg gaaaaggcag
    11281 gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac gtactagatg
    11341 caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta caaacgatgt
    11401 tgacttctga atctacgatg aaagatgtca aactggcagc agctcaagga gattctgttt
    11461 cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga cggccttttt
    11521 tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac aaacggtttg
    11581 atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc attgaatctg
    11641 taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat ggaaaaaaag
    11701 tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc agtcatatca
    11761 tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct gatctccaag
    11821 aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat cagctaatca
    11881 gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg ttggacttct
    11941 ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa ggaacgactt
    12001 atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa acaaatgaac
    12061 agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg caataattat
    12121 cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa ttctcttcaa
    12181 aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa ggtattctaa
    12241 aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa acaagagagt
    12301 catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc ttatagatag
    12361 accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt aagttctttt
    12421 ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca cctgaccaga
    12481 gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga ctggtggtgt
    12541 ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta agtaagaaat
    12601 agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat tagcttggat
    12661 ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta tcgtcatcgg
    12721 aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta aatcagcaat
    12781 ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt tgatctgcgg
    12841 ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt caaacaaacg
    12901 ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat aatccattta
    12961 ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac ctacttatat
    13021 gttactacac acccttattt tttacacttt aacagtcacc atcatcaata gtttggagat
    13081 tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata tgaacgtttc
    13141 attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac taggagaaaa
    13201 agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa aaagggcaag
    13261 tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg ccgtttcagc
    13321 ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg gtaactatgg
    13381 cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa ctgtgaatgc
    13441 acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt ttggtacaga
    13501 gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg attctttcgc
    13561 taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg ttggcagtaa
    13621 cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc ttgatcactt
    13681 aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt tcgagcagga
    13741 atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag ctaaaacata
    13801 tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg atttatcaga
    13861 taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata cgccacttca
    13921 aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt cttctgcatt
    13981 gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag aagatttttc
    14041 ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa tcagtgctcc
    14101 ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa accaagacgg
    14161 aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg atcctaacga
    14221 aatatttatc agcgacaatg gtacagatac gacagacaca agcgacagta cagatacatc
    14281 taccacaagt gatagctcag attcatca
//
LOCUS       DASJUF010000063        14222 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJUF010000063 DASJUF010000000
VERSION     DASJUF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.063"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1835)
                     /locus_tag="U4B62_001800"
     CDS             complement(<1..1835)
                     /locus_tag="U4B62_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2141956.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="U4B62_001801"
     CDS             complement(1832..2536)
                     /locus_tag="U4B62_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2141957.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="U4B62_001802"
     CDS             complement(2548..4857)
                     /locus_tag="U4B62_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2141958.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="U4B62_001803"
     CDS             complement(5052..5516)
                     /locus_tag="U4B62_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141959.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="U4B62_001804"
     CDS             complement(5516..6142)
                     /locus_tag="U4B62_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2141960.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="U4B62_001805"
     CDS             complement(6149..6514)
                     /locus_tag="U4B62_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2141961.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="U4B62_001806"
     CDS             complement(6504..6842)
                     /locus_tag="U4B62_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141962.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="U4B62_001807"
     CDS             complement(6832..7158)
                     /locus_tag="U4B62_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2141963.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="U4B62_001808"
     CDS             complement(7136..7417)
                     /locus_tag="U4B62_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2141964.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="U4B62_001809"
     CDS             complement(7419..8783)
                     /locus_tag="U4B62_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2141965.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="U4B62_001810"
     CDS             complement(8796..9365)
                     /locus_tag="U4B62_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEN2141966.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="U4B62_001811"
     CDS             complement(9337..10524)
                     /locus_tag="U4B62_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2141967.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="U4B62_001812"
     CDS             complement(10588..12315)
                     /locus_tag="U4B62_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2141968.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="U4B62_001813"
     CDS             complement(12312..12764)
                     /locus_tag="U4B62_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN2141969.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="U4B62_001814"
     CDS             complement(12876..13256)
                     /locus_tag="U4B62_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2141970.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="U4B62_001815"
     CDS             complement(13253..13639)
                     /locus_tag="U4B62_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141971.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="U4B62_001816"
     CDS             complement(13641..13919)
                     /locus_tag="U4B62_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141972.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
BASE COUNT         3937 a         3057 c         2240 g         4988 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 tt
//
LOCUS       DASJUF010000064        13978 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJUF010000064 DASJUF010000000
VERSION     DASJUF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.064"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..460
                     /locus_tag="U4B62_001817"
     CDS             140..460
                     /locus_tag="U4B62_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141973.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="U4B62_001818"
     CDS             912..1112
                     /locus_tag="U4B62_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2141974.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="U4B62_001819"
     CDS             1259..4048
                     /locus_tag="U4B62_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2141975.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="U4B62_001820"
     CDS             4095..4622
                     /locus_tag="U4B62_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN2141976.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="U4B62_001821"
     CDS             4643..5833
                     /locus_tag="U4B62_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2141977.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="U4B62_001822"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="U4B62_001822"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN2141978.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="U4B62_001823"
     CDS             7966..8640
                     /locus_tag="U4B62_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2141979.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="U4B62_001824"
     CDS             8637..8978
                     /locus_tag="U4B62_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141980.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="U4B62_001825"
     CDS             9008..9367
                     /locus_tag="U4B62_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN2141981.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="U4B62_001826"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="U4B62_001826"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2141982.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="U4B62_001827"
     CDS             12286..13752
                     /locus_tag="U4B62_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2141983.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3939 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgttttt
//
LOCUS       DASJUF010000065        13866 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJUF010000065 DASJUF010000000
VERSION     DASJUF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.065"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..172)
                     /locus_tag="U4B62_001828"
     CDS             complement(<1..172)
                     /locus_tag="U4B62_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2141984.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFL"
     gene            complement(651..1370)
                     /locus_tag="U4B62_001829"
     CDS             complement(651..1370)
                     /locus_tag="U4B62_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2141985.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1419..3065)
                     /locus_tag="U4B62_001830"
     CDS             complement(1419..3065)
                     /locus_tag="U4B62_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2141986.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3244..3834
                     /locus_tag="U4B62_001831"
     CDS             3244..3834
                     /locus_tag="U4B62_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2141987.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3926..5431
                     /locus_tag="U4B62_001832"
     CDS             3926..5431
                     /locus_tag="U4B62_001832"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN2141988.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5515..6867)
                     /gene="celB"
                     /locus_tag="U4B62_001833"
     CDS             complement(5515..6867)
                     /gene="celB"
                     /locus_tag="U4B62_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2141989.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6867..7385)
                     /locus_tag="U4B62_001834"
     CDS             complement(6867..7385)
                     /locus_tag="U4B62_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141990.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7401..7727)
                     /locus_tag="U4B62_001835"
     CDS             complement(7401..7727)
                     /locus_tag="U4B62_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN2141991.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7740..9719)
                     /locus_tag="U4B62_001836"
     CDS             complement(7740..9719)
                     /locus_tag="U4B62_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2141992.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9740..10054)
                     /locus_tag="U4B62_001837"
     CDS             complement(9740..10054)
                     /locus_tag="U4B62_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN2141993.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10222..10515
                     /locus_tag="U4B62_001838"
     CDS             10222..10515
                     /locus_tag="U4B62_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN2141994.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10593..11705)
                     /locus_tag="U4B62_001839"
     CDS             complement(10593..11705)
                     /locus_tag="U4B62_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2141995.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12092..12271
                     /locus_tag="U4B62_001840"
     CDS             12092..12271
                     /locus_tag="U4B62_001840"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141996.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12465..12635
                     /locus_tag="U4B62_001841"
     CDS             12465..12635
                     /locus_tag="U4B62_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2141997.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13059..13799)
                     /locus_tag="U4B62_001842"
     CDS             complement(13059..13799)
                     /locus_tag="U4B62_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2141998.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4330 a         2831 c         2372 g         4333 t
ORIGIN      
        1 ccaagaaccc atctgctgaa atatgaaaat ttttgaatgc tacgacattc gccattacag
       61 gtatgtaaaa gaaaaaaatc atccaaagca tacccggtac agccatcagg acaagtgctt
      121 tataacgcca tagattcata aaaaaatgat tgaacccctt tcgttttttc atctaaaaac
      181 ccctttcgat tcattcgcaa cgtaattata gcgctttcaa taatgagaat aaataaacaa
      241 agtataggga aaatgaaaca aagtatagga gaggttgtga aatcagcgtt ttggcctgag
      301 tattagagaa aaaaacggga aaatagttcc tcatattttg ccgttttttg agctaatacc
      361 gagggtcagc tgattgaaca ccgtttatca gaggttgtga attcagcgtt ttggcctgag
      421 tattagggag atcgtgtcct tactatctaa ctctgagtca caaggaacaa tggggactgt
      481 aacaaaagtc ttgtcacagt cccttattcc gaacaaacgg tgttccagaa gtagcttcta
      541 cggaaataag ccgaaatttc acaaaaaagc catccgaaag tcagacggac aagtctgctt
      601 ttcagatggc gctacaacta ctctgcttat tcttttagct atccattcaa ttatttttct
      661 ctcacttgtt ctagctcttc tttggtcatg ggtcgatatt gccctttatt caaatcttta
      721 ggcagccaaa attttcccat acggattctt tttaaatata agacttcttt tcctactgct
      781 ttgaccattc gcttaacttg gtgaaacttt ccttcatgca aaatcagacg gataattgaa
      841 gtttctgtct cttgatctac ttgatcaatt tggagttttg ccggcaatgt ctgctcatcg
      901 ccatcaataa tcagtccttc tgcaaataaa cggatatctt cttctgtcat tataccgcta
      961 acctcagcta gatattcttt ctccacatgt tttttaggtg atagtaactg atgggcaaac
     1021 accccgtcat tcgttaaaat caagaggccc tctgtgtctt tatctaatcg gccgactgga
     1081 aaaagatctt cccgataatc ctggtcttcc aataaatcga ttacggtttg atcatgatta
     1141 tcctgagtag cagaaatgac tcctgcaggt ttatgcaaaa tgtagtaaaa atctttttga
     1201 tacgtaatga tttctcctaa ataagtaacc gcatcttcga attcatttac ttgcgcttta
     1261 tcgcttttca ccacttgtcc attgacttcg atcgttcctt ttttgattaa tcctttgact
     1321 tctttgcggc tgccaagtcc catatctgct aaaaacttat ctaaacgcat gcgtttcccc
     1381 catttcaaaa gagcatcaat ctagctcttc tgattcactt attttatttt taatttatgt
     1441 cgtaaacgcg ctattcctgg tcctagtagt ctttctgcca agcggatttt caaagctaaa
     1501 taaccataca ctaaagcccc tatacctgct actacaagaa tcaacaatag agactggaat
     1561 ttgctatctt cgtttaaaaa agcaccaaaa atcatctttg tgatccacga agccaaaagc
     1621 ataatcaagg taagaataaa gatcagcaat gtcctgcgcc agacaaaact tggtttaaag
     1681 cgggctactt catggattct ctttaagatc aaggtacaag acacagcaaa tccgatcatt
     1741 gtcgccaata aagggccgta tacttcaaat aaacgaatgg ctgggtattg aagaaggagt
     1801 ttcactagaa acccaataac caaataatta attgccgcct tgttttcaaa cattccttgc
     1861 agcatgttgg aaactagcat atatagtcct aggaagagtc ctacaaaact tgcttggatc
     1921 agtacattac ttcctagaga atccggtgta taaaataatg tatttagcgg ataggcaagt
     1981 aacataacac caaatgtcgc cggaaacata aaaaatgaaa acaactgtaa atttccgctt
     2041 gtcaattttg ccagacctac tcgatctttc aacgtaacag cttctgtaat caacggtaac
     2101 ccagtagacg cgatcgatgt tgccaatgca ataactacca tcgtcaactt atctggattg
     2161 gcactaaaaa tcgaaaaaag atccagtaac tgtgcattgg aatactcggt ggatgcagac
     2221 atgatcttca taaaagtgaa ctgatcgact aatttgaaga tcgttacccc tgaacccaca
     2281 ataataaacg gaatcgcttc tttaacggta tctaacaaca attccttcgt tgcaatattt
     2341 attttgtttt cgctgtagtg aataaacgcc gatgtatacg cttgatgttt cttcaaaaaa
     2401 tacaaaagga caagaatact tgctagcata ccgataaatg ccgcgaaagt tgattgcgtg
     2461 accgccgtca catagtcacc atccatgact ttcatgatga tgaatgtagc caatagcata
     2521 taaaaaatac gtgccacctg ttctacaatc tgcgacagcg cataaggcat catttcttga
     2581 ttgccttgca aataccctcg tatcacactc atacaaggaa aaaccaatac agcgatactc
     2641 aaagaacgca tcgttggaat cagttcttct ccgccaccag aagcatgagc tagccaagga
     2701 gaggcgatgt acatgaacaa agcaaacaac aggcctaacc cgcccatcat ctgtaatgca
     2761 cggataaata atcgtcgtga agtgccatat tcattcaatg aattatagcg tgcagtttgt
     2821 tttgcaatcg cagcaggtat tcctgctgta gagatcagta gaaacaatgc atatatattg
     2881 tatcccatat tgaataatcc attggcagcc ttagcatttt cgcccatcca agcataccag
     2941 ggaatgatat agacagcgcc tagcaatctt gaaatgatat tactggcggt catccaagca
     3001 gagcctcgtg ccattttttc ttgcggtgtt aattgatcta atgctggttt tctttgatta
     3061 tccatcggtc agattccaac tttctacaaa acattactat cattttaatt ggtaaaacag
     3121 aaactggcaa tcgattttat ataactttta taagaacaat tgtttttttg aattcggttt
     3181 cgtttttttg gtaaagtaaa gaaatataca gccttttatg gctaacattt ataggagata
     3241 tcaatgacac aagaaataaa aaaactaccc gttatatttt taggtctttt ctttatcaca
     3301 gttggattga attggttctt gctccctcac gatatagctt ccgccggagt gggcgccatc
     3361 ggacatttag ttgaaacggc cttttctatc caacgctcag ttactgtttg gagcatcaat
     3421 cttatgatgc tgcttctagc cgctgtccta ttaggaaaat cagtattttt taagactgta
     3481 gcgggaagtc tgctttttcc tgttgtttta gagattgttc caaaagtgga aatgatctcc
     3541 tcacatttct tttcactgat ttcaggaagt cttttgttca gtcttggggt ttacacattg
     3601 tacacagtcg gggcctcaaa tggcggtata acgataccgc cgattatttt ccacaaattt
     3661 tttcggcttc caatgccgca aggactttta cttaccaata gtcttatcgt ctttttgaat
     3721 tattatgttt ttggattgtt ggagacacta ttcgttctcc tatctattac agtcagttca
     3781 ttgtttatga aaatactcat ccgcttgtcc cctgtgtcaa gaaaatttga ataaagctgg
     3841 attttgtcat agcggaaaca aacgatatat taaaaagagt gtgctataat aaaccaagaa
     3901 attgaataaa gcgagggatc aatcaatgag cttctcttta gaagacatcc gtcatttact
     3961 attaaaagaa aatctattaa aagaatttgt ttcaacaaga ggctggcatc ttgatgtacc
     4021 agaaaaagct gaatttcttc agttaagtta tgattcaaga aaagctgatg cttctacttt
     4081 gtttttctgt aaaggaatga atttcaaaga agaatatttg gattcagcaa tcgaacaggg
     4141 tattcaatat tatgtatcag aacaaccata cgaaaattca gcagtaggaa tcatcgtaac
     4201 ggatatccgt aaagcaatgg cgcttcttgc tatggctttt tacgactatc cgcaaaataa
     4261 attgaaagtg atcggattca ctggaacaaa aggtaaaaca actgctgcgt actttacaaa
     4321 agcaatcttg gatcacacaa caaataaaaa aacagcattg ctttctacta tgaatacaac
     4381 gttagatggc aaaacatatt tcaaatccca tttgacaacg cctgagtcat tagatttata
     4441 tcgtatgatg tcagaagcag ttgaaaatgg tatgactcat ctagtgatgg aagtttcttc
     4501 acaagcttac aaaacacaac gcgtatacgg cttgacttta gatgtcggaa tcttcttgaa
     4561 tatctcgcct gaccatatca gtccgatcga acatccgaca tttgatgact atttctattg
     4621 caagcgacaa ttgatcttga attcaaaaac agttgtttta aatcatgaaa gtgactattt
     4681 tgatttactt agggaaacag ccgatctatt ccagattcca acgatcactt atggtcgttc
     4741 ggaagaaagc aattatcaag tgatccgttc agataaaggt actcacggtt ttactttatc
     4801 ttctcatgaa gatcgcttag ctatctgcga tactgcttat gatattttgc tggctggagg
     4861 attcaaccaa gaaaatgcag ccagtgcgat cattgccgca gcactaacag gtgcctccag
     4921 tcaagatgca caagacggat taaaagaagc tcgtgtacca ggccgaatgg atcaattgat
     4981 ccaatccaat ggagcgcatg tctatgtgga ctatgcacat aattatctaa gcttgaaaac
     5041 attgctgaaa tttgccaaaa atgaacatcc agatggccgt gtaattgttg tattaggcag
     5101 cccaggaaac aaagcaatct cccgtcgaca tgattttgga aaagtgctat cagaaacagc
     5161 tgacatcgcc tttttgacgg cagatgaccc agcatttgaa gatccgaaaa aaatcgcgga
     5221 agaaatcaat gaagcaatta ctaatccaga tttgatcgtt cattatgaaa tggatcgtcc
     5281 tgaagcgatt cgacgcgctt tagcagaaag cgctccgcaa gattcagtcg ttattgcggg
     5341 aaaaggcgtg gatccctatc aaaaaatcaa tggtgtagat gaaccttatg aaggggatta
     5401 tgcaatcgtg aagcggttga ttgaagaata atggtattta tatactagtg gtttcccatt
     5461 tctaagaaaa aaagggactg tgattttttg tcacagttcc ttttttctta gaaatcattt
     5521 taacttttca ctagctaatt ctgctgcctt ttctctttct tcatttaata atttattatc
     5581 atagaatttg ataaatggga accagataat gaatgcgatg attgcacaaa tgactgctaa
     5641 cacagcagct ttccaatcac caccactacc aacaaatgct ccgatcccga ctggtgaagg
     5701 ccacggcatc tgtgcaagca tcggtttgac gatttccagc cgaatggcaa aataagcgag
     5761 tgtagcagaa gccatcggag ctaagaaaaa cggaaccgct aaatagggat tgtagacgat
     5821 tggcatcccg aaaatgattg gttcattgat attgaataat cctggtacaa gtgaggcttt
     5881 tcctaacact ttcagctgat cagatttcgc gaggaatgca ataaagatga tcaatcctaa
     5941 agtcgcacct gatccaccaa cggttacata agaattgttg aattctcctg ccaacggata
     6001 atttgcgcct tgtgcgttag ctgccatatt agctaatacg atcggggtca agaaagaagt
     6061 gatgatattc gctccatgga tccctacgat ccacaatgca tggatcaaga aataaatcac
     6121 catgacacca agccatgtat tcgtcaactg cgtaacaaac ccaaatggga tagcaatcat
     6181 tttgaagata tccgttccta gtgcaactaa taatccattg atgatcatca ccacaaacgc
     6241 aatgacaaat gttgggatca gagcagtgaa agaacgagaa actcctggcg gaacaacatc
     6301 tggcattttt atgacccaat ttctcttgac acacatacaa tacaattcga ccgcaataat
     6361 cgacataata atagctgtaa agattcctga agttcctaaa cgttcgacaa aagagcccat
     6421 cctaatacca tttactacag tttctgtctc attgatagaa gcaatcaagc tgattttacc
     6481 atcagtcatg atcaattcag ggatacacat aaagaatgca aaaacagata gtaatgcccc
     6541 cgtgagcgga ttaacattta atttttgttc atctcgttca attgacgtta attcataagc
     6601 aaacacaatg gcaaagtaaa tagataaaat ccccatcgtc attgtattga cgatcatgta
     6661 caagtcacta actttgaaaa aagttgcatt aaaaaatcct tctaaaaatg ggaacggtaa
     6721 tggcaaaata tttaaaacca aaaacatcga gcctacaatc gtgaaaggaa tagaagccat
     6781 ccccgctgcc atgacagccc gaacgatttt aagttgcgca attttcccca taggtcccat
     6841 caggtatttc tgcaaaaaat caaacattag ttattctcct ttcctaaata taacgctttt
     6901 tcatattgtt taatacggat atactgttgg tcttggttga gtgaaatttt ctttaaacga
     6961 tactgtataa atgcactggc aaaaataccg cttacaacaa gaatgaaaac aaataactgt
     7021 cctccattgt tatttgccat aaaaggatac aagaatgtaa atcctgaggt aaacaacact
     7081 ggaatcacta ataaattaaa gacactctgg aacaaaaaat accactttgt ccatggaacg
     7141 ttcgtttggt gttttcgata aagctttact tgttctccta ctgcaggcaa aatagctaaa
     7201 atcagaatca tcggtaaact acttcctaaa cttttagctg atagaagcag aatcatccat
     7261 tgcatgttca tgaaaaagaa aaaggcagtc aggtaacgta cgattagata acgattgaaa
     7321 tacatgtttt tcaaactaag ttgctttttt tctaattcga ttttcctttt tactgtatcc
     7381 tgcacaatga tccttccttt ctatttattt ttttgataca aataattcat ttcctgtgca
     7441 acttccaata atgtcatcgt cgtcatcaga tgatcttgtg catggaccat gataatttcc
     7501 atttctattt ttgtcccgct ggcatattct ttcagaagtt gagtttgtga ctgatgcgca
     7561 tgaagaatct cttcatttgc ttgtcgcaac ttctcttcta cttcttcaaa catctcttta
     7621 cgcatcgagc gaaatgcttc atgtatcagt gtccgtgcat ttccgctatg gaaaatgata
     7681 tcaaaagaag tcacttgtag tgtttcactg tttatttttt cagccatctg ttttcctcct
     7741 tattcgatca gtttgataaa tttactgctg aaattctcaa aaataggttc agctaagatt
     7801 tcctgctgta cttttagctg gtcaactaac ctaacgattg ctttagtcac gaccgtaatt
     7861 ccttcatttt cgacataaga aggcgaaact aaaaaaacaa aatgaatttc ttgatattga
     7921 tcccactgca tcccagaagg aatcaatgca acggctacct ttgtttcgat tccaactgga
     7981 acagcgggat gtgggactgc cactgtatca ctgaatatga tttgtcccat attttcacgt
     8041 tgctggatct gctttcgcat ttctcttata tatccttctt cttcatttat gctcagcttt
     8101 tgcagcaaat catgaagaat ttcttccttt ttcggttcgt acgtataagt cttgaaaaat
     8161 ttttcagaca actgttcggt gtaaatctgc tttttttgct gtagagaaaa tgctggtatc
     8221 ggtctatctg aggaatgata gggtatagat tctccaacta cctttcgtat tttacgaaca
     8281 tcatcttcat tcaaaaaaat actgacatgt aaaactggaa ttttgaaaaa cattgtcgaa
     8341 agatcaatag aagaaataat caggtcgatt ccttttaaag ttgtttcatt gatctcataa
     8401 tagcctttta cacttaccac tgtaatattt ttaccgaatt cactgactac gcgattcttt
     8461 aatagttggg cactgccgta acccgttgca cagataatca atgcatgtag tttatgcgca
     8521 gctctttctt tttccaatgc cgccatgaga tgcaaagcca aataggccca ttcatcttca
     8581 ttgatggtat agtttttcaa agccggcatg ttgctgaaat gcctttttac tgcgtcaaag
     8641 actttgggat tttctttctt tatctcctca gtcaatgggt tctcaatgcg aatacctcgg
     8701 ctcaatcgaa tcatcatcgg tgacaaatga tcgagtagtc cattgaaaag ctggagatcc
     8761 tctactaatg aaaaatgaaa ttcctgagac aatcttttca gaactttcgc taattccttt
     8821 gatagttcct gatcattttg attttctttg tgattcgact tagccatgag atgtaatgtc
     8881 aaataagcaa tttcttcttc gggaaacgat acacctgcga ttacctcaat acgttgaacg
     8941 atccgctgcg ccacttgata ctctactcgc ttagaaactt cttgttcgat gcctaattcc
     9001 ttgatttcaa acccttctcg gagccgtttg atccccaaag ccaagtgtaa agtcagattc
     9061 tgtatgataa aatccgaaac ttttaactta gcttctcttg tttcatcgag aatgataatg
     9121 actagctctt caaaactgat gtctgggaaa aaacggcttt gccctaaata ttctttcaat
     9181 gaatttccat aatggtttcc aaaaaaagta tccatgataa aatgccgtcg atttctttct
     9241 tccccttcga tccatgtccc ttgaccatgc ttagaaacga tttttagtga atatggaatc
     9301 aatcgctcct tgatttcttg catgtccttt gtcaaactgg aacgactgat ataaagctct
     9361 tctgccaaat catccataaa aagaatggca tcttccaaaa gcaattggtt caatatatac
     9421 ttttgtcgat cttcggactc ataaaatcct tgcggttctc tagcatatcc aggtactatt
     9481 tcttgccgac tcaaaaacaa gtcaaatgtc agtctttgta aaatatgcag ttggtagcca
     9541 taacctggtt tagcaatgat cttacctccg tttttttcta ctaactcttt gatccgctgc
     9601 aagtaattac ggattgtccg atcagataat aacaattcag aagctaaacg ttgactggtg
     9661 acatactcct tcttgtgatc aatcaaatat ctcaacagca atttttcctt ctgtttcatt
     9721 atcatccact cctattccat catatgttct cttcgatcac ctctgctaac ttctggatgc
     9781 ctgtagggat tgggacatac gcttggaaag ggatactaac aacaggcttt ccaacagatt
     9841 ctcctaattt tttgaattta tccaaaaaca tcgtcgtttg cgggctgatc agaaataaat
     9901 caaaatcact attcttaatc atattatcgc cttctgtagc tgaaactgca tctacgagta
     9961 cctcttctct tttttgctta aagaaatccg ttgtctttga ggccatcatc gaggatgaca
    10021 ttcctgccgc acaaataatc aatgcttttt tcataaatca atcactcctt ttatttttat
    10081 aaaccaagca tatcttgtaa gcgttttcta ttcaaaatat atatttccgt agcattgcgg
    10141 aaaaaatgtg tatcatatct tgaataataa tttctggcac cgtgatatac tagtgataga
    10201 attttttcag gaggggattt agtgagtatc acagtgaata ttttgtatac aagtacaaat
    10261 ggacaagcca gaaaatttgc agaagaaatg gtcgaaagaa acattgtcaa agcgattcgt
    10321 gcagaagaag gaaacgagaa atatgactat tactttccac tagaagatcc agaatctctt
    10381 ttactgatcg accgttggaa agatgaagaa gcaatcgaaa agcatcacaa atccgaaatg
    10441 atggctcaaa taaacgaact ccgtaaaaag tataaattaa aaatgaaagt cgaacgtttc
    10501 actgatttac cgtaaaaaga tacacatgga atgatagcaa tttatcatcc atgtgtatct
    10561 tcactttttc cttatttttt attcgatctc atctaatgat tcaaaccgtg tttttctctt
    10621 atttttcgga taagtatttc agtcacccca atatacatga ttcctaaaca taccccaagg
    10681 atattctcaa aaatgcggat cgttactgct cctggaaccc cgaataaact ggcagtgatt
    10741 gccaaagcac caaaagaatt aaaaactgtt ttatatctat aggtagaaca aacacctaaa
    10801 gctagaccac ctaatatacc gacccagtta aaaggaataa attggctgat accaataaat
    10861 aaaacacatc caatcaatga gccaatgatt cggtctactg ctcgttcttt gaaaccaatc
    10921 gacatcaatc catagctaga tacaatagaa gaaaatgcaa accctgccca catcaatcgc
    10981 tgaaaaggga catactctcc tgcaacgatc aaaagactaa tgccaaatgc atagatgagc
    11041 atccaaattt tttcctgcga atacatgcct ttcaaaaaaa tctctttaaa caagcttttg
    11101 tctttgttct tttctcgatg ttttctgtaa aggagaactg aaaaccaaca aaaaaacaaa
    11161 aataataagc tacttgtttg tgtaaagaaa ttcttgttca acgaatcttt aggtaacgaa
    11221 taaacaataa agagatatga aaagccgtac aaagaagcat tccccatttt gggatcacta
    11281 gcggttgcca ttaatatgct gcttagtaaa ataaaatgaa tcaacatgcc cataactgac
    11341 catttgatca gctggacata cggtgctact aatagtgaga acatgacgat tgctaaacct
    11401 atgatcgaat gggaaatctt ataaccaaag tcaacaaaac gaatactcat caacatacag
    11461 aataaaacca cagcgtatgg tttatgcttc gattcaaata atgttgtaat tataatgata
    11521 aaacaaatgg caaacaatac gatgaataat gatcgtaaaa acaatgaaca aatatacatg
    11581 cacttttctt ttttagaagc tgcttctcgt attttttgtt ttaaaataaa cggatctagt
    11641 tgtagagact gataaaaatc cacctgttgt cgtcctttct ttttactttt attttttaca
    11701 gtcatctatg acaggttgta aatactatgg aacatcatat ggcgttgtca ttttcttgtc
    11761 aactattttt cctattcatg tttttataaa aaaacatatc gttttcttgt atgattaaat
    11821 caccacaaaa taaacaactg agagaacgat atgctttatg aatggttcta tcaaaaaaat
    11881 tctgagatca atagacaaaa atttgatgat tacagaggtc tcttacgaaa ccttacagaa
    11941 gaaaaaaaca ttgatcgttg ctgcagttct cttccctgct ccatgtgtct gtagaaattg
    12001 tggttctgct gtggtagatg gaaataggaa attaatggtg gtgaaaaaca agaagaagga
    12061 aaccattgtc cactttgaat aatataatca tatgccaatg gttatacgcc taaaaaagca
    12121 gcgctatacc tgtaaaaact gtcgaagcca ttggaacgct caaagttatt ttatccgtcc
    12181 aaggcactcg atttcaaatc atgtcagaca taaaatcact tctttactga ctgaaaaagt
    12241 gtcgttattt tttatttcga aaagttgtta ggtatctttg acaaccgtta ttcgcacatt
    12301 gaaagagttg agaagctatt taccaaagtc ttccaaaaga attctgtctt gagtgttgag
    12361 ggttgtgaat ttcgttctca cgtttccgtt gaggataaac tgagttttat ttccgcggat
    12421 ggcgagacag aaaaattaat cgagaaaatt tacctacaca aaaaattcct cgattaacaa
    12481 actatttcct tagctgcact aatcctaaag aaatagaatt cttagttaca gacatgaacg
    12541 ccacctactt tcagctcacc aaacatgttc taccaaatgc gaaattggtg attgatcgat
    12601 tacttggttt ttccacgcct ttcaaggagg cgtaaccttt attcacgagt tggttgaagc
    12661 tttccgagac aaaagacccg acttgttttt ctccttattg acaaagagcc attaaaacta
    12721 gagaaaaaat tacaactgat cgctgattat ttgtagaaag aaaatcaaag tattacccaa
    12781 taaacttgag tcattaggca aaaaacactg cgaaactcaa gaatgatttt cgcagtgttt
    12841 ttgtatattt tttattaact agtattacta caaaggttta tttttcactt ctcaacgcag
    12901 cacaaaattg tctttactta tttactggtt gggttcaacc ttctctactc cgatactaat
    12961 atttgtttgt ataccccttc taaaatcggt agtttccatt caattgatgg gtactagttg
    13021 attttaaaaa ctatgcttat tcaatttttt attttagctc atatatttcg tttctttcta
    13081 aattttatac tgatgaaaac aagtagcaca cccaatattg acaatataaa ctggctaatg
    13141 atggtttctc cagtttttgg aagatgctta tttccactgc ttgttggatt gtttcctctt
    13201 atatctgagc tatcagcaac ttttctattc tcttttgtta aaaagatcgc cacttcttgt
    13261 tctttttcga tcttgaaagt tactggtgtc gcatccaatt cataccccgt tggcgcttga
    13321 gtttctacca gctgatatgc tcctggtgct aagccatcta tcgccagttt tccatctgct
    13381 cctgtggtta gacctgactg cagaacggtt ccattggcat cttgtaactc aaaaacagct
    13441 ccttgcagga cttctccgct ttggtcatcg atctttgtca acacgacacc gcctggtgta
    13501 agtcgatttt ctttcgtcaa ttctaccacc gctgtctgac ttctttcgat ctcaaactcg
    13561 attggtgtcg catccaattc ataccccgtt ggcgcttggg tttctaccag ctgatacact
    13621 cctggtgcta agccgtcaat cgccagtttt ccatctgctc ctgtggttag acctgactgc
    13681 agaacggttc cattggcatc ttgtaactca aaaacagctc cttgcaggac ttccccgctt
    13741 tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa gtcggtttgc tttcgtcaat
    13801 tctaccaccg ctgtctgact tctttcgatc tcaaactcga tcggtgtcgc atctaattca
    13861 tacccc
//
LOCUS       DASJUF010000066        13817 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJUF010000066 DASJUF010000000
VERSION     DASJUF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.066"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            158..352
                     /locus_tag="U4B62_001843"
     CDS             158..352
                     /locus_tag="U4B62_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2141999.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            621..920
                     /locus_tag="U4B62_001844"
     CDS             621..920
                     /locus_tag="U4B62_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142000.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1017..1451)
                     /locus_tag="U4B62_001845"
     CDS             complement(1017..1451)
                     /locus_tag="U4B62_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2142001.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1683..2393)
                     /locus_tag="U4B62_001846"
     CDS             complement(1683..2393)
                     /locus_tag="U4B62_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2142002.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2383..3243)
                     /locus_tag="U4B62_001847"
     CDS             complement(2383..3243)
                     /locus_tag="U4B62_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN2142003.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3248..3886)
                     /locus_tag="U4B62_001848"
     CDS             complement(3248..3886)
                     /locus_tag="U4B62_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN2142004.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3901..4200)
                     /locus_tag="U4B62_001849"
     CDS             complement(3901..4200)
                     /locus_tag="U4B62_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2142005.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4243..5727)
                     /locus_tag="U4B62_001850"
     CDS             complement(4243..5727)
                     /locus_tag="U4B62_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2142006.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5747..6208)
                     /locus_tag="U4B62_001851"
     CDS             complement(5747..6208)
                     /locus_tag="U4B62_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2142007.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6226..7293)
                     /gene="ulaG"
                     /locus_tag="U4B62_001852"
     CDS             complement(6226..7293)
                     /gene="ulaG"
                     /locus_tag="U4B62_001852"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN2142008.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7511..8281
                     /locus_tag="U4B62_001853"
     CDS             7511..8281
                     /locus_tag="U4B62_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2142009.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8554..9381)
                     /locus_tag="U4B62_001854"
     CDS             complement(8554..9381)
                     /locus_tag="U4B62_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142010.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9949..11184
                     /locus_tag="U4B62_001855"
     CDS             9949..11184
                     /locus_tag="U4B62_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2142011.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12183..12443)
                     /locus_tag="U4B62_001856"
     CDS             complement(12183..12443)
                     /locus_tag="U4B62_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2142012.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12470..13771)
                     /locus_tag="U4B62_001857"
     CDS             complement(12470..13771)
                     /locus_tag="U4B62_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2142013.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4440 a         2829 c         2175 g         4373 t
ORIGIN      
        1 agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa ctttcaaatt
       61 gattcttttt atttatcata aagatataga tacataatca ttttcactta catatatttg
      121 aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag aagtaaaaac
      181 aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc ctttacttgg
      241 ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa agaacgagct
      301 agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt aaatccaatg
      361 tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt atagaattag
      421 aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac catatgatta
      481 tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg aatcaaatat
      541 acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta taatgtatca
      601 gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa ctacttaagc
      661 aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa gtgacagagt
      721 atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca aaaggaatat
      781 acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct ttaaaaaaaa
      841 atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt gataaagagg
      901 tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac taagtctatc
      961 ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat tatatgttat
     1021 ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga aataccaccc
     1081 atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt ctcagtatca
     1141 tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa agcttcattc
     1201 aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat atcttgaata
     1261 aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt cccttcccca
     1321 ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc agtgtctttt
     1381 gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat cataagggaa
     1441 tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat acagttcttt
     1501 gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc cttaaaattc
     1561 ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata aattttcttc
     1621 acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt accctagcat
     1681 ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt atctagtaaa
     1741 tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc catcagacaa
     1801 atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc ccatgtgaac
     1861 ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt tctttcctta
     1921 aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac ttcttctttc
     1981 gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt cgtgccataa
     2041 gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg agtatgagta
     2101 atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag atctgaagat
     2161 ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac catttgatct
     2221 gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc tagctcacgg
     2281 tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg caatgccaga
     2341 tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga catcatagcc
     2401 agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc ttcgaaagtc
     2461 ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta gacgtttcaa
     2521 atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat cacgaaactg
     2581 gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag tgatataatc
     2641 gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca aattgcctag
     2701 gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta aaaattttga
     2761 aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc catatttaga
     2821 agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat tcattgattt
     2881 ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta gattatgcgc
     2941 caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg gatcagcaga
     3001 accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta caccggagct
     3061 gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc gtgctaaccg
     3121 ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt ttacaaggga
     3181 aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc caatttgagc
     3241 catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg gccttcatcg
     3301 gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg atcccttcaa
     3361 ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag cctagttcaa
     3421 tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat aacagcgcat
     3481 cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc atggcttgtt
     3541 caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt tgtgctgcct
     3601 ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct gcttttgcaa
     3661 cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact agttttttat
     3721 ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag caaagaattg
     3781 tgcccacttc aataatatct actacatcac caactttcat aacatctcct aatgcatgtt
     3841 ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta gttccttcct
     3901 ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt ctttgactga
     3961 catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag taaacatatt
     4021 caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt ttccctctga
     4081 aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg ctttttccac
     4141 acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta aaattctcat
     4201 cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc tctgcttttc
     4261 tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt tccgatattg
     4321 taactgcgga atagccaata aaccaatgac aactacggca attccagcat aacctaggac
     4381 tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc cgatataacc
     4441 accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta cttgtatgat
     4501 tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat tggcataaac
     4561 tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga tcgttggtga
     4621 tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa acaagacccc
     4681 aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa ttcctggaac
     4741 agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt catcgacgaa
     4801 tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag aagtcgtcat
     4861 aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca aataatcttt
     4921 gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag tagcaaccat
     4981 attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt ttttgtcttg
     5041 cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta attttgcaaa
     5101 tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt ctgtcaattc
     5161 ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta aaatcagtcc
     5221 cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta aaatccaaaa
     5281 agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac gcagtttcgt
     5341 atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta ataaaatcat
     5401 cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag cattttgtcc
     5461 aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc caaccaagat
     5521 tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt aaccgaccgt
     5581 tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa tatagcctaa
     5641 taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg caaaatacgt
     5701 ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa ttatattctg
     5761 ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca accaatccat
     5821 ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct ggatcttttg
     5881 ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca ctttcaaaac
     5941 ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca tcactttttt
     6001 ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg tatttttcta
     6061 cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg atcaatacaa
     6121 gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg taatgaatca
     6181 attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata aaaacgacgg
     6241 aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa aattaaactc
     6301 aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga tataaggttt
     6361 aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct cttttggatc
     6421 agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg cattcaatga
     6481 ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc gtgggttttc
     6541 accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg caaataaatt
     6601 ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga ataaatagtt
     6661 tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt ctggatcttc
     6721 acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga tgtctttgat
     6781 tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc cccatttttg
     6841 ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat tttgatgaac
     6901 tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga ttaaagcgtc
     6961 tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat taggctgcat
     7021 attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc cattcccatg
     7081 actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt catgactttt
     7141 cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat ccactacttc
     7201 tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta ctaaccactt
     7261 ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat atcgttgttc
     7321 caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt atatgaccgt
     7381 ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa taaagacaga
     7441 tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg tccctcagga
     7501 ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag aaattttgga
     7561 actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc ttgatgtatc
     7621 tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga tcatacgcaa
     7681 ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat ctggtcctga
     7741 aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt atgatacgct
     7801 gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat ctaaccattt
     7861 gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata attgtagtta
     7921 tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg acatcgcatt
     7981 aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg gagtcgatgt
     8041 agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa tgatggtcaa
     8101 caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac tgacatcaaa
     8161 gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat tttgttctga
     8221 aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag attcatttta
     8281 agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca aatttatcga
     8341 taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta tccatccaat
     8401 cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta aataggaatc
     8461 aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc gaataaacgg
     8521 tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt caatcacctt
     8581 taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat atctttctaa
     8641 ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca agtattccaa
     8701 tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata attggattgc
     8761 tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata caatgcagac
     8821 catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca cataaacttt
     8881 cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag ctgcttgtaa
     8941 gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa ataatgctgc
     9001 atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa tttgatttaa
     9061 aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat aagattcttg
     9121 gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga ttttgtcttc
     9181 ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa gcgcacttaa
     9241 gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg cttgttcaca
     9301 aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt cttttagtaa
     9361 tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt tctcctttat
     9421 atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat aaaaatttta
     9481 attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt tctttttgtg
     9541 ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca tggcgagaaa
     9601 gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat gacgacagca
     9661 cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat ttttaatgac
     9721 acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg ttgcgtgttt
     9781 ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt gtgaaaacag
     9841 cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg ttttttagta
     9901 tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat gaagacaatt
     9961 ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga ggttgccaaa
    10021 gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt tgctcctttg
    10081 atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa aaaacaagaa
    10141 gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac caaagaaatg
    10201 gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc tcgactgatt
    10261 gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat tcctttagta
    10321 tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc gctgatgccc
    10381 cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac aaaaaagcgt
    10441 atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa agaaatgctt
    10501 cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat cacaacgcct
    10561 aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg tccaattttt
    10621 gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg aaagcaacaa
    10681 gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg tatgttacgc
    10741 aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc tattgactat
    10801 ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata tctgtctagt
    10861 catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg gaccgtccaa
    10921 actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga gcaaagctgt
    10981 aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc agtgactgaa
    11041 agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca gcaggcacgc
    11101 acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga aatcgaaacc
    11161 ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat agagactcat
    11221 cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa atagtggatt
    11281 gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc cttaaaataa
    11341 aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct tagtcaaatt
    11401 ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa gaccataata
    11461 attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc gctttgtaga
    11521 gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt accactttat
    11581 ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg atgcatactc
    11641 ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat gttctatgtc
    11701 gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc ccagcacaat
    11761 tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat taccattcat
    11821 acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat ttgacttgat
    11881 cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc cattttttcc
    11941 tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa agggctgtga
    12001 caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct tgcatctgcg
    12061 gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta agaagcaatt
    12121 ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca cttttagaac
    12181 atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt gatagcaaaa
    12241 attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg tacaatgatc
    12301 tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt gatttgaata
    12361 tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa gataaaaaca
    12421 aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact tagacagtct
    12481 ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca taagctttta
    12541 caaatggata atagataata aatgctatca atgtgttgat catgatcaag ataacaacac
    12601 gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc catgttggca
    12661 aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata atagtcaaaa
    12721 ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata ggagctccaa
    12781 aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga ccaacctctt
    12841 tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc gttgctcctg
    12901 caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag ggtaattggg
    12961 tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc agccacattg
    13021 gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac aacaaatgga
    13081 tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg gttaaaggct
    13141 cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga atcagccaag
    13201 taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca acagcaggtg
    13261 gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga tatacttcca
    13321 ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa gaactcatag
    13381 gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga acttgtaaca
    13441 taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca tatgatttag
    13501 ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc gtcatcctaa
    13561 agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca ggaaacggta
    13621 aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt gaaacaaccc
    13681 cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt gggacaagtt
    13741 tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa taaaatatga
    13801 accattcatt gcatgac
//
LOCUS       DASJUF010000067        13324 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJUF010000067 DASJUF010000000
VERSION     DASJUF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.067"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..1000
                     /locus_tag="U4B62_001858"
     CDS             131..1000
                     /locus_tag="U4B62_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN2142014.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            1020..1367
                     /locus_tag="U4B62_001859"
     CDS             1020..1367
                     /locus_tag="U4B62_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142015.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1380..2642
                     /locus_tag="U4B62_001860"
     CDS             1380..2642
                     /locus_tag="U4B62_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142016.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2722..4098
                     /locus_tag="U4B62_001861"
     CDS             2722..4098
                     /locus_tag="U4B62_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN2142017.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            4095..4724
                     /locus_tag="U4B62_001862"
     CDS             4095..4724
                     /locus_tag="U4B62_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142018.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            4736..5206
                     /locus_tag="U4B62_001863"
     CDS             4736..5206
                     /locus_tag="U4B62_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142019.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            5203..5685
                     /locus_tag="U4B62_001864"
     CDS             5203..5685
                     /locus_tag="U4B62_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142020.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            5682..6344
                     /locus_tag="U4B62_001865"
     CDS             5682..6344
                     /locus_tag="U4B62_001865"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN2142021.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            6331..6699
                     /locus_tag="U4B62_001866"
     CDS             6331..6699
                     /locus_tag="U4B62_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEN2142022.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            6780..7232
                     /locus_tag="U4B62_001867"
     CDS             6780..7232
                     /locus_tag="U4B62_001867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142023.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            7884..7937
                     /locus_tag="U4B62_001868"
                     /pseudo
     CDS             7884..7937
                     /locus_tag="U4B62_001868"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7941..8540
                     /locus_tag="U4B62_001869"
     CDS             7941..8540
                     /locus_tag="U4B62_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN2142024.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            8556..9032
                     /locus_tag="U4B62_001870"
     CDS             8556..9032
                     /locus_tag="U4B62_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142025.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            9049..9951
                     /locus_tag="U4B62_001871"
     CDS             9049..9951
                     /locus_tag="U4B62_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142026.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            10024..10215
                     /locus_tag="U4B62_001872"
     CDS             10024..10215
                     /locus_tag="U4B62_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142027.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            10379..10966
                     /locus_tag="U4B62_001873"
     CDS             10379..10966
                     /locus_tag="U4B62_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142028.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            10966..11865
                     /locus_tag="U4B62_001874"
     CDS             10966..11865
                     /locus_tag="U4B62_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEN2142029.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            11865..12779
                     /locus_tag="U4B62_001875"
     CDS             11865..12779
                     /locus_tag="U4B62_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142030.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            12831..13046
                     /locus_tag="U4B62_001876"
     CDS             12831..13046
                     /locus_tag="U4B62_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142031.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         5096 a         1744 c         2724 g         3760 t
ORIGIN      
        1 catccttttt taaattggct atatatcaag gtttatagtg ggaataaata aaaatacatt
       61 tgacaaaatt tacaatatag tttattatat atttatctta ttatgataac acaataaaag
      121 gaatggtggt atgaaaaaga agaagtttag tcgatcgttg ttaggaattt tggttttagt
      181 tgcggttggt cttagtggca agggttactt tcagaataac agcactcagg aacatgattt
      241 tcagatcaaa agtagtgttc agataggtaa taaagcagac agtgaatcaa aagaattttt
      301 agggatacgt tatgatccaa acaaatatcc aaataatatc gtaaacatta atgatgggaa
      361 atatacactg acaaaagaag accatgaagc attatcagaa aaaggcagtc aaaccttttg
      421 ggtaaattat gcagatttag atcaacttgg aagagcagga gatgttgaag cgttgatcac
      481 ctatgatgct gtaaattctc actcatctaa aaatgtggaa cgtccatctt tcaaaagttc
      541 tgtccatatt gcaggagaat acaaggatgg agtatttgat caagagagag atacttgggt
      601 cggcaaagat tcaaacaatg aaatcactca gctagaaggc tataaaggtt atctctataa
      661 caaaagtcat tcattagcat ggtcgctagg aggagatatg gaggtacaca atttaacact
      721 tggaacgaga gcgcaaaatg ttgggaaaaa tgatcagaat ggagggatgg cttactcaga
      781 aacgattgta cgtgatgcaa tctataaaaa taagaatgaa aaagtattgt ataaagttga
      841 tccaatttat aaagggaatg aattagttcc tcgtggttct catgtcgaag ctttctcagt
      901 taacgataat ggaaaaacgg taaaccttaa tgtttatgta tttaacaatc aaaaaggaat
      961 caacatcaac tatcaaaatg gtacttgggt aagagattag aaattgagaa ggtgaagaat
     1021 tggaaacaat aagcatcaat ggattagata aatcagttgc aaatcgaata aaaaaaggaa
     1081 atggctacta tcttctgaag aagggtaaat attcagttgc tagagagtta gaaacaatga
     1141 gtatcgtgta tgtagctaag aatcattttg acgtagaaca ggctattttt gatccagatt
     1201 ctgaaatagg gaaatgtgtt gaattatcag agaaaactgt tagtacaaaa ggggcgatgg
     1261 tcattgagag agaaatgtta gacgaaatca gagtaatcat tcctaaagat acgcagatgt
     1321 gtgaggacag cataagtgac gatgaaccaa aatcagaaag aaaataagga gatattcaga
     1381 tgagcgaaaa atccattgaa ttaaaggaat ataagcgaag cgatggagaa aaatattatc
     1441 ctgtagccag tttttctaaa tcagtatatc tggttggacg tgcattggta gttggtgcaa
     1501 ttggcatata tattttgcat tgggtaacaa aggttgtaca actgcttgta gataccttag
     1561 taagtattct tgtctcgtta aatgatgtta acggaacaga tgatttagga ttaaaagaga
     1621 tgtttaaaga gttactcaat gcgttttttc ccaattattt aagttattca tataatgtac
     1681 aaagcttttt tgcaaagcta cacttggtag acatctctat tttagtatta gaagcaggaa
     1741 cggtattctt tgttggatat ttggttactt tgatgttcag gcatcactat ggagaacaaa
     1801 gaccattttt tgatgatatg gaaagtagaa ggttgaaaag agaagctcta gaagctatgg
     1861 atgtgaagta tgatccaaac aagattatag gagatgaaac gaacaacaaa aaaagaaaac
     1921 gaaacaaaac gcaattagaa tccgatgtgc tatcaacgct taaaaatatc aaaatttctg
     1981 ttcatacaag acgtgatcta agtgtcaatg aagatattcg agaatatatc gtaaaaatcc
     2041 cgcaaccaaa agggaagaaa gttagagaaa cagttttacg acaaattcaa gatttagggt
     2101 cgatcctgac aagagcaaca agcggacgag ccaaatttgg tgaaaaagaa gatgaagttt
     2161 caggcgatta tttcattttc cgagcatcag taaatattga aagagaacgt aaggaaagag
     2221 taagtaccaa aaacaaagaa acggaaagag caacatcttc aatcgcagtt tcaaattcac
     2281 aattttcttt tccaatcaat ctctttgccg ataattccaa aaatatccaa aaccaaaccg
     2341 aaaaagctag aaaatatgcc gaaactcaaa agcaatcttt agaactttat ctatcttctg
     2401 ctgacgtacc tgcaagtcta acagatatgc acgttgggaa tacatctatt gaatatttgt
     2461 ttactttatc tcgtaccaga aaaattcaac taagctcaat gaaagaagaa attgaaactt
     2521 acttgcatat atcaggtgta atcgtgcgga tcaacgcagg gcgtattcaa attactttac
     2581 cattaggaga aaatgatcgt attccaattg atgtaccatc aatgatcaat agcgtgtttt
     2641 agaagacatc acaaatagaa acagaaaata caaactacca gttcacaaac agaaaagtac
     2701 cgagaaaagt gaggaataga aatggctatg aaattattag agaaagtcaa aaagagagcc
     2761 gaaaaagctg aatctatttt gctagataaa gaacctaata taaagcttaa tggaaaattc
     2821 agtgtcaata tgttgaacac aaaggctaaa gacccaacag atgctatttt tggaattaag
     2881 cctgatggca caattgctca tttttgcatc agcgaaatgc cacacatgtt ggttgcagga
     2941 acgacaggat cagggaaatc agtattactg aatgaaattc ttgttacagt aatgtgtcac
     3001 tcaacgcctg atgaattaaa aataggaatt gtagacccaa aaagggttga atttggaaga
     3061 tacaaaaaat tgccgttcat gttggctgat ccaattacag atatggatga agcttatgat
     3121 ttttttgaat atcttgtgat cttgatgcat gaacgttaca aactgatgga aaaagcaggg
     3181 ttacaaaaca tcacgctata taacgaatat gccgaaaaaa atggattaga tagatttccc
     3241 tatgtgatct tattagttga tgaatattca cagttggttg gaacacataa agaagtagaa
     3301 ggtttgattg ttcaattagg acaaatggca agggcagcag gtattcatgt tatcttagca
     3361 acacaatctc ctcgatcaac agttgtcact ggtattatca aagccaattt tccgtcacgt
     3421 gtttgcctta tggtagcaag tgatttagaa agtaggattg tattagatga aggcggtgga
     3481 gaaagtctaa gtccaaaggg agatatgctt attaagcttg ttgatggaag tatggttaga
     3541 gcgcagggag cttacatcag taacaaagaa attgaaacaa tcttcaatca ccttagaaat
     3601 actatgccag aaccagaata tgttgattac cgagcaatcg tagctgaaac tcgtggtgag
     3661 ggcggtgaag acgaggattt tggtggaatt ggtaaagctc ctgtaagaaa atctgctaaa
     3721 agaaatttga acacaccttt agcaaatcca tcatctaaag agccatcaat tgaaaagaag
     3781 agtgcgtttg ctattaagct tgaaaaaaat aaagaagaaa aagctaatca gggagctaag
     3841 tcactagaaa aacttcagca agaaaaatta gaaaaactag aaaaagcatt atcatcatca
     3901 acttctgaaa ctcaatcttc aaagggtata gataatggac aatcaattac aactaaaaac
     3961 aagcctaaaa atagtaattc aaatctctcg gtaaatgttg ttatcacaac tgataaatct
     4021 gaaaaaaaac aagtgaacgg aactacttca cccatcatgc aaaaaatgat aaaggcaaaa
     4081 aaacaggtgc agaaatgatt atcattagtt cctttttgag atggctaaca ccatataaat
     4141 atacagatga aatagttgag aagcgagcga agaaaaaagt taaaaaaacg atgttaactc
     4201 ctaaagaaat gctagatcgt ggaatattgg gagtgaaggt tttagaagta aaccaaccgt
     4261 ttgaattgga taatgcgaga gataggttgc ttagagctga agttgatgtc gcatactttt
     4321 atcatgataa aagcaagcat caaatagcta tcaaattcaa tccaaaatcg gcatggcgat
     4381 accaagcaag agatgaccta aagcgtagga agctgaaacc gatgctctat cctgaaggaa
     4441 aagaggcatt tgatagaacc catattgttc ccattggatt tcatggtaca gagtcagata
     4501 accgcctatt ggtagggtgg aacagctcac aaaatagagt tcagatcaat aaatttgaga
     4561 agaaggtaag aaaatttaac gatcggaaaa taatactttg gtttgccaat attcagaagc
     4621 aaccagatgg gacagcaaag tgggaagcgg tcatatttga tgaagatggc aaaaaagtaa
     4681 tgtctgaagt atggcatgat aaagctaaat ttgtttggca gtagaggtga aaattgtgtt
     4741 aactaaccta aaactatata gagagggatt taacgaaaaa gtaagtatcg gtgaatctat
     4801 tgaatattta ggaaagaagt acattattat tcgtattctt gaaattaaaa aactttattt
     4861 tagtaaatat aataatttag cagttagagt tctcgtccaa aaggttggaa atccaagtga
     4921 ctatagaagt gattaccata actataaaac agaatcatct gtaattaagc gttataatca
     4981 atcaaaaaat gatgagagaa tattcaaagt aggagaaatt attatttcta agtatgggat
     5041 agcatacgaa atagtttcta ttaaaaaatt ttattatgaa tttgttgatt tggttgtaga
     5101 atattctgtt cgattgatcg ttccgtggag taaagaagaa attaatgagg cattaaaaca
     5161 tgaacgaaaa agtacattca aagttttaga aggtgaaaaa aagtgaaaac agaccttact
     5221 aggcaagctg agaagtgctt atggcatgaa acaaatcgca tgggtgtatt tggttgtttc
     5281 gaagtcacta tcggttggga tggcagtgaa agagtagatt atataactta ttcagtagat
     5341 aacacagttc gatgttatga gatcaaagtt actttagctg atttaaaaag caaggcgaaa
     5401 cagactttta tcggtgatta taactattta gttgttacta gtgatttgtg ggagaaaatg
     5461 caacaagacg ataaacttat gcggaaatat tactatcaag gcgtatacgt gttcaatgaa
     5521 aaatatccta gcataataag tgttaagaaa gcaaggaaac aaaaagtatc gctaggtaca
     5581 agatcaactg tattagaaag catggttcgt tctatgaatc gtgaagttaa gaaactctat
     5641 aaagtaaaac cattttggga gatagcagag gtggaaaaag gatgattcaa acatttgcaa
     5701 aaatgagtag aagtgcttgg attgaaaagc tggaaaatga tttgagtgtt atgcgattag
     5761 ctccaaagaa attcaaacga gagattgcag aaaaagaagc aattttgtcg gtattgaatg
     5821 cggtagaact ggacgaaccg aaaaagtcag ttgatgtacc tgaaaatgta gcagagttac
     5881 tagattatta tcgtaattcg tcagatgtcg atttgttagc tctgatatta acatttcacg
     5941 attggtatta ctgtcaaaat gaagatactc agcatgataa acatgataaa gcagttgact
     6001 ggttaataga gaatccagaa aagttcatgc gtgcttggct agatggctat gatagcaaga
     6061 aaaagccact ggaaagtaat gaggaaacgt atttaacggc agaacaatat gactatgcta
     6121 gaaaaacagg agattgggaa tctggtaaga ctttcaatca cagaaaagat caaccaaaaa
     6181 ttagtttttc agatatagcc gcaattatcg aaggtatcta ctatggacaa gccgatgaag
     6241 agatcttttc tatacaagat ttattagaat ttttgaacaa aaataaggat agctttgtag
     6301 aactaactga ttatgaggag gatacagcaa atggaaaatt atgatccaaa tatgcgatgg
     6361 ggtacacata cgcttaaagt ttcttttcaa cgttgggatt acaagggatt tgtgactttt
     6421 agaaaagctg gaaattgtaa aggattagat gtcttagcgt tggatgaaga ctatctatat
     6481 gaccatccat tgacggataa tccaatcgga tttggattgc ttcctgaaga tgatgagggc
     6541 aatgaatggt tcaaaatgat tcttacaaac gataaaggtg atcaattatt tgtagaagat
     6601 acttggtcat atttgagtga atatattgtt agcattaaaa tcattgattt tgtagcagat
     6661 aaagaaaaag agattggaga aggtaaatct aattactagc taatgaatag caactttgga
     6721 ggataagtca aaagaataaa aaaagaatac tgtgtgtcat tgcagggatg tatctttgca
     6781 tgatagtatc tgcggttacc atttattggt taggaaatca gcaaaacagt ttaattgttg
     6841 atctggggga gttaaaggtt ggattacgaa tgactagatg gcttttatta gctcaaacag
     6901 ttggtttagc catattgact tggatatgtg tggaaaaaat aacatctaat aataatcaag
     6961 agattgttga atcaacaaac ctaagaataa gaataactga acaagcaatt gaaaagcaaa
     7021 aagacaaaga aaaaaaggaa ataatgtcaa tttatttatc tagtggagca tcattttttc
     7081 atgaaataga ttcgatgaaa ctgagtagtc aagaaatcat tgatagatgg aaaaacatgc
     7141 ctctaggtgg tgagttaatc gttgaagata gtgatggttg gtcagcttat aagaaggacg
     7201 agattgtagc tatcacgatt ttgtttgaat agtaatatat taccactaga taatctgaat
     7261 atgttgatct gctagtgacg taccaaatgc gtttaataga accttggtca caggcagaaa
     7321 ttgataaagc agtaaaattg aaccgattat caaaatttaa cgtattagga aatgcctttt
     7381 aaaaatcgca atacacaaac agaaaaatac caaaaaatta gcaatgaacg taggtaagaa
     7441 catgacaata aataataata accctaacaa atataatgct cttgtccaat gctatatcga
     7501 aaacaaaatt acaatgcaag aagtcgctga tgaattaggc aaatcaaaaa gcctagtatc
     7561 acacgacttt aagcacaatg tttttccgaa cccgattgag ctgaggaaaa aatttcaaaa
     7621 gagagtttat caagaaatag ggaagtggat tgatgaaggg aatgcaattg accctttaat
     7681 tgaaaaatat ccttttttaa aaagtaaaaa taaaattcaa aggttaattc gtcaaggcga
     7741 tattcaagct aaagaacctg ttttgacaac caatgatttg gctaaaataa atcttcataa
     7801 acagattatt gaagactttg aaaaaaatct ttcaacaact gaaattttaa aaaaatacaa
     7861 tattccgaga ggaaagctaa catcgactct ttttagagaa tatggagaat cggcagttac
     7921 taaagcaaag gaagtagcga atgagagaaa aaaacaaaaa tagtagtcga tacctagtag
     7981 cgactgtagt ttttgtttgt gtattgattt tcctgtttga ttttttgcag attggaaaaa
     8041 gcataattgg aaatacattg agtgcaaagg aaatggttgc tttaggtggt ttgtatgaag
     8101 gacatttgtt cccgaatata ttcatttcta tcgtttctca tattaacttg taccatttaa
     8161 tttctaattt attgttctta gtagttcttg aagaagcact gaatgatcaa tatgagtggt
     8221 taacactatt gattgtttta atcatatcag gacttctagg aagcttagtt ccttttctgt
     8281 tacaaagcaa catcgtaagt atggggctta gtggaggtat ttttggatgg attgctttat
     8341 ttgcgattgc atacatccgc tttgatagta aggaacattt attactaagt gtttttatat
     8401 tattgataat cggcatttta ctggtatcta cttttgaatc aggcgagaca aacgtcttgt
     8461 cacatgtaat cggatttgtc tgtgggatga tcttgggatt tattctaaag aacatttcat
     8521 tgatcaagca aaagaattaa aagggagtgc taaaaatgcc aaaaaaaaaa gaaaagaaaa
     8581 ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca aatactgtta agaaaaaaga
     8641 ttgaaacaat agacgaagaa gttagaaata aaagggctat gtacgaacaa cgtgaagcgc
     8701 taagagaacg aatccactat tttgatgata tacacactaa aatctatgaa gcacgtacaa
     8761 ataacgaaga cgaaagcaaa ttaggattag ctcaacaacg aatatttgaa tggctaaata
     8821 cgaatactga gcaagaaccg ttaagagcaa ttagtaatct ttttgaattt ggaacgaagc
     8881 attggtttaa cgcagtagaa aaggatgctg acttacaaga agcctttgac acgctaagta
     8941 aggatagcaa atccatgcga gatttattga ttacttatct aaatgagcat gagtttgaac
     9001 aaacgaaaac caaaaagaaa aataaagaat agaaagttgt gatgaatcat gctattaacc
     9061 ctgttaggtt atttatgttt aattcttgct ttttttgggg caatcgctat aattattggt
     9121 caatttcaaa caaataatcc gattatgcgt aacataggca ttggaattgt gattgtaacc
     9181 tttattgtgt ttaatgccac taagattttt ggaagtaacg ataaacaatt tggtatatcg
     9241 ggagttaata atagttatgc tccagattct ttgggaaggg tagtaataga tggaacattt
     9301 gaaaatgtaa cgagttataa aaatgtttcg ctgacagtga aggaagatgg caagaaagca
     9361 tcagacgacg ttctcgctac tattaagccg tctcaatcac aatttgaagt gttttataca
     9421 gttaaagaga atagccctgt acacaagctg acattggttt ttaacgttga tggagaagaa
     9481 tactataaag atgtaaaaat aaataaaaca tcaacgaaaa agattatcga atcagcgaaa
     9541 aaagaagtag ctacagaaga aagtacaact gaatcaagtt ctactgtcga agcggaacta
     9601 ccagataaaa ttgattttga tcctgaaaaa gtcaaatcgt ttgaatctgt ttctggttct
     9661 atggctgatc ggtttaacac tgaagtcttc aaagctatca aaactgttaa attggatact
     9721 gaaaagcaaa tgattaatgt taccttattc aattcagttg aatctatgga tcaggataaa
     9781 aaagttacgg ttgcaagaga actaggaaaa agagtaattg gtttgtatcg tggtactgct
     9841 aaaccagatt cagacggtgc agatttgcca gttcatgtag tttatgaaga cggtaaagtt
     9901 tttggagatt ctacagcaaa cgaagcatta acgattaaat tggctaaata gccagaaagt
     9961 tataataaac aaatggtgtc acaccaataa tatataggtg tgacaccttg gagatgatag
    10021 ctgatggtag taaataaaga aaaagttaga aagtatgtga ctttaaaaat agcttctgtt
    10081 aaatatttag aaaagcgagc taaagaagaa gaaagagcaa gaggtaagaa agtaacagtt
    10141 tcagagttgt tagaagaatt gatcgagaat gatcaaacaa taagacgtgc atttactaat
    10201 aggtctatca gataacaata taattaataa aaaagaaaga gtgatttggt cttagactaa
    10261 atcactcttt ttatataaag tttctttaca ctcattccga ataatgttaa aattctttat
    10321 atgatatata gttccttgtc agaggaagag ataaccagca atattggagg gataataagt
    10381 ggaaaacaat aatgatatta gacattttag cattggggaa agagttacaa cagtaagtga
    10441 attatcaaag gattatagag ataaagaggg aacgattatt tctcttaata agaatcgaag
    10501 aggcgtttgg caatatgttg ttgatttagg tgatggcgtt acagttgagt gtaaagatag
    10561 tcaactgatc agaaataatg gcagtcattc cattgaaaaa gcaattcttg tatacccaaa
    10621 agtatcaagt aagatcatga atgactggtt gcttggaaaa tttggtgagt acaataaaaa
    10681 agaaatcgca gaagtgatcg gcaaatctta tccaacagta ttagggtatc taaaaaagat
    10741 aacgagtaaa gaagcaaaaa ttgaggatgt tttaacagta agagagcttc aatcattaat
    10801 cgagtatcgg aataaacaaa taacaaatcc tgagtacctt agaaaagaag atggtctatt
    10861 gaaacaattg gaacaagtaa ataaaatccg gaagatgcaa aaggtagctc ctttaagcct
    10921 tgataccttt aaaaaaatct atttgaaaga gatcaaaaaa gactaatgtg gactgtaact
    10981 ttaccgttcc aacctgtatc tgcaccaaga ccaagtgcaa gaaggaatgc tgctgatgaa
    11041 actaatgacg tagcagagaa aaggatttca acctactatg ataaaaagta tcttgattat
    11101 ttgaaaaaaa taaagagctt tatcgaagat caagatttat tagatgatga attttactcg
    11161 attgtaaatg atcctatggg cgcaattatg gaagtggact tttactacca gataccaaaa
    11221 agctataaaa aagtctataa cattatgaag acaacagcgc cagatttaga taatttagta
    11281 aaaagtgcta tggatggtat ttttaacatc tcagctatac gagatagcca tatcacaggc
    11341 ttaattgctt ttaaatataa tgtggcgaac gaaggaaaaa cagttgtgag gatcaaacga
    11401 tattcagaat ttgagtggga tacttctgaa ggattgaacg gagatatatg ggaagctaca
    11461 tttgatttta aaccagtagc aacgccaaga ccaaagtcta aatttagagg aaatgggatt
    11521 atcacttact atccaaaaga gtacaacgac tatttggaaa atatcaagaa tacgcttaaa
    11581 gagaaagatt tggtaaatga tcaactgaaa aaggtaatga atgctcctat gggggttata
    11641 gctcagatag attattttta ccaagtgaga aaagataaga aaaagcttga ttcactgatg
    11701 cgtacttcac agccggatat tgataattta gtcaaaggaa cattggacgg aatttttaac
    11761 aaggaaatag gcatcaaaga tagcagggtt gtagggttaa ttgcttttaa atacaatact
    11821 tttgaaaagt cgaaaacaaa cgtgagacta caggagatgg gctgatgaat aataatgaaa
    11881 aaattcttca tgtacttgat agctttgaaa taattcaaga agaactaaaa aaatatcggg
    11941 atgttttgga acaaagatat gattttgtca atcagcaaaa gagtaatcac atggatttca
    12001 ttctcaatat gaatgacttg aagaagaaat tagttgaacg aaaagaacaa gaaaagttga
    12061 tcaaagctta ctttgaactc ggtgaaaaag aagttaaaaa tgcaatggaa ctgaatgaag
    12121 agcgcagggt tttagatcag ctgttagaac aattattggt gatgtttcaa aaaggacgaa
    12181 tagatgagga tttaattgaa gaaggattga gaaaatatcc ttcaaactca ggtatcggaa
    12241 tagtgctgaa agcgattgat gaagaagaaa ttgaagcttt cataccgcca gaggattttg
    12301 aatcggcaat ggaatatata aaatattata gccaagggat cactgctttt agagagtttg
    12361 atcctgaaga tgttattcat gacttgaaca acctaaaaga atggtgtgaa agttatggag
    12421 tcgatgattc cggattggac tacttgatca gtattatgga aatcgaagaa gagatgccgg
    12481 ataagcctga tccaacagat attttagaat tgatccatga agctagaaat ccaattgcct
    12541 atatttctag aggatatact gtattggaat actataagcc ttatatatca gcgatgaacc
    12601 atttaagacg tgtattaagg gaaaaacgtg aataccgctc agtgttgaat gcaacgaata
    12661 gattagaaaa agctgtttca gagctagatg cttattatcg tgaacattac ctgcaagcag
    12721 gtggtatgcc aagaaatacg aaagcaaata tcagtaggta tataaaaaaa gctgagtagg
    12781 cggagttagc ccataaaatg gcaaaaggta acctgaaagg ggcattttga atgaaaaggg
    12841 atgtttttga attgtacgta ttagaacgtc tagaagacgg catgatggca caagagataa
    12901 ttgatgaaat tgatgatatg atcagcagat ttggtcaatt ggagttgaat gatggaaaca
    12961 aaaattcggt agtgctaaaa caattttggt tgaacaattt ttcgtataag gattttatca
    13021 aagaaaaggc aaatacattg tactgactaa attcacgagg agcagtgatg agagcttatt
    13081 gctaagagca gttaggctct tttttgtttg tgtaaaatta tttgttttat aatacacatt
    13141 tatacacaaa aaaatattga cagatagtac acaaatgtgg taggatatat ttgtaagcaa
    13201 gttagattaa caagttaaag gaagagcagt tatgaaaaaa gaagaactaa tagatatatt
    13261 tcaaattgtt gaacgagcaa ataatatggg aattatgttt tttgatcgta tttctttaaa
    13321 gatg
//
LOCUS       DASJUF010000068        13215 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJUF010000068 DASJUF010000000
VERSION     DASJUF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.068"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="U4B62_001877"
     CDS             complement(105..629)
                     /locus_tag="U4B62_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2142032.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4B62_001878"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4B62_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN2142033.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4B62_001879"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4B62_001879"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN2142034.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U4B62_001880"
     CDS             complement(2351..3031)
                     /locus_tag="U4B62_001880"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2142035.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U4B62_001881"
     CDS             3157..4026
                     /locus_tag="U4B62_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2142036.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U4B62_001882"
     CDS             4052..4567
                     /locus_tag="U4B62_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142037.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U4B62_001883"
     CDS             complement(4614..5207)
                     /locus_tag="U4B62_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2142038.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U4B62_001884"
     CDS             complement(5232..5411)
                     /locus_tag="U4B62_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142039.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U4B62_001885"
     CDS             complement(5488..5997)
                     /locus_tag="U4B62_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142040.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U4B62_001886"
     CDS             complement(6093..6473)
                     /locus_tag="U4B62_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2142041.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="U4B62_001887"
     CDS             6803..7552
                     /locus_tag="U4B62_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN2142042.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="U4B62_001888"
     CDS             complement(7873..9090)
                     /locus_tag="U4B62_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2142043.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="U4B62_001889"
     CDS             complement(9348..10625)
                     /locus_tag="U4B62_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142044.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="U4B62_001890"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="U4B62_001890"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN2142045.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="U4B62_001891"
     CDS             complement(11547..12491)
                     /locus_tag="U4B62_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN2142046.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="U4B62_001892"
     CDS             complement(12493..13179)
                     /locus_tag="U4B62_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN2142047.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3744 a         2765 c         2391 g         4315 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgtta atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    13201 caatattttt ttaat
//
LOCUS       DASJUF010000069        13126 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJUF010000069 DASJUF010000000
VERSION     DASJUF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.069"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            90..1829
                     /gene="yfcC"
                     /locus_tag="U4B62_001893"
     CDS             90..1829
                     /gene="yfcC"
                     /locus_tag="U4B62_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN2142048.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1986..3170
                     /gene="iadA"
                     /locus_tag="U4B62_001894"
     CDS             1986..3170
                     /gene="iadA"
                     /locus_tag="U4B62_001894"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN2142049.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3281..3484
                     /locus_tag="U4B62_001895"
     CDS             3281..3484
                     /locus_tag="U4B62_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2142050.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3585..4247)
                     /locus_tag="U4B62_001896"
     CDS             complement(3585..4247)
                     /locus_tag="U4B62_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142051.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4260..5762)
                     /locus_tag="U4B62_001897"
     CDS             complement(4260..5762)
                     /locus_tag="U4B62_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2142052.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6310..7989
                     /locus_tag="U4B62_001898"
     CDS             6310..7989
                     /locus_tag="U4B62_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2142053.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8116..8595
                     /locus_tag="U4B62_001899"
     CDS             8116..8595
                     /locus_tag="U4B62_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN2142054.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8814..9494
                     /locus_tag="U4B62_001900"
     CDS             8814..9494
                     /locus_tag="U4B62_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN2142055.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9502..10833
                     /locus_tag="U4B62_001901"
     CDS             9502..10833
                     /locus_tag="U4B62_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2142056.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10848..11810
                     /locus_tag="U4B62_001902"
     CDS             10848..11810
                     /locus_tag="U4B62_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN2142057.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11921..12259
                     /locus_tag="U4B62_001903"
     CDS             11921..12259
                     /locus_tag="U4B62_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN2142058.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12338..13045
                     /locus_tag="U4B62_001904"
     CDS             12338..13045
                     /locus_tag="U4B62_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN2142059.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4168 a         2319 c         2881 g         3758 t
ORIGIN      
        1 tgtggaaatg tcatttttac tgtataattc atttgattat ccatttttta ttcatcaaaa
       61 ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac aaattcttca ggaaaaccga
      121 aacgcgactt cactaaatta tcgacacccc atacgtatgt cattattttt ggtgtagtca
      181 tctttgcatg gatactgact tttgtcgttc cagcaggaaa gttcagcacg caagatattg
      241 aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt tttacgtcaa gattccttcc
      301 gttatgctta cgagctggat aagtcgtatg tgtttgatca attagaagaa ctgcaagatc
      361 atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg attggaaaaa gtcattgctg
      421 acggagaaaa aaatcttacg caggaaaaac tagatgaaat ctccttgaca gatgatgtgc
      481 tttacgatca gtacggcgaa aatatctatg atacttctaa aaaattgcat aaaacagcca
      541 aaatatgggg aacagatgat tttggcggct ttggtttctt aaattttgtt tttgaaggac
      601 tagtatctgg agataagtat ggatctgctg tagggatcgc tgcattgatc cttgttgttg
      661 gaggtgcttt tggtattatt atgcgtaccg gagcgatcga cgcaggtatc tatgcgttta
      721 tcagtaaaac taagggactg gagcgtttag ctttaccgct attattcttt gctttttcat
      781 tcggtggcgc tacattcggg atggcagaag aggtaattcc attctcaatg gtgatggttc
      841 catttgtcat tgcgttagga tatgactcga tcgtagcagt aaccgtgact tatgtcgcat
      901 ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag cgtagcggtg gctcaaggga
      961 ttgctggaat ccccgtgttg tctggtgcga cattccgatt gattatgtgg gtagttgtta
     1021 ctgcattagc cgctggttat atgatgatat atgcagaaaa aatccgtaaa aaaccagaaa
     1081 gttcgttgac ttacaaatca gatgattatt tccgttctca tatcaaaaag acatctgatg
     1141 aaaataaaaa attcatgctt gggcataaat tgatcttatt agagatgtta gttgtccttg
     1201 tctggatcgt ttggggcgtg actcaaaaag gctattacat tccagaaatc gcttctcaat
     1261 tctttgtgat gggattagtt gctggaatca ttgctgttct tttcaaatta gatggaatga
     1321 aagtcaatga catcgcttct tctttccaat ctggtgcagc tgatttagct ggtacagcaa
     1381 tcgtagtagg tatggccaaa gggattcttc ttgtcttggg aggatctgat gcatctgttc
     1441 cttcagcttt gaatacgatt ttgcatggga tcgggacggc gttgaccggt gttccagctg
     1501 tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt caacttggtt gttacatcaa
     1561 attcaggaca agcagctttg actatgccaa tcatggctcc actggcagat ttagtaggtg
     1621 tatccagaca agtagcagtt cttgcttatc agcttggttc aggttttatg gacgctttta
     1681 cacccgtatc agccagcttg attggtgtgc taggtgttgc acggatcgaa tgggcaaaat
     1741 gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt tttaggaact attttcatca
     1801 tgattgccat tgcaataggt ctgcagtagt actggagacc tgtaaataaa aaagtaatcc
     1861 aaacagtaga taaggactcc aagaaccgtc attttacggt aaaatcaaag gagtaaagat
     1921 cctatcgttt ggattatttt ttaagatcct atcatcagtt atacttaaag aaagaggaaa
     1981 aatgtatgaa aatcattcga caaattgaag tgtttgcacc agattactta ggaaaaatgg
     2041 atgtactgat tgccggtggg aagattttgg ctgtagaaga acagctagaa ggcggctacg
     2101 aaggagtgga agtagaagaa ctttcaggtg agggaaaagt actaaccccg ggtttcattg
     2161 actgtcattt ccatatttta ggtggaggag gagaaggcgg ttatcaaaat cgaacgccag
     2221 aagtcacatt gagccaattg actacagctg gtgtaacgac agttgtcggt tgtttgggaa
     2281 cagacggaga aggtagagat atgactgcgt tgatcagcaa agcgaaaggt ctggaagcag
     2341 aaggaatctc cacttatgta tacgaaggtt cgtaccgatt gccggtaaaa acagtaacag
     2401 attctatcat caaagacttt ttgacaattg ataaaattat cggaatcgga gaaatcgcag
     2461 tatccgatca ccgttcttct cagccaagtt ttgaagagtt cgctcgtgca gtagcagatg
     2521 ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat caatgtccac ttaggcggag
     2581 gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga aacagaaatt ccgatcacac
     2641 aatttttgcc tactcatgca aatcgcacac cagaattatt tgaagcttgc gtggcatttg
     2701 ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga tcctgatttt tgggagaaga
     2761 cagatggtga agttcgcttc agcaaagcac tgaaacgatt gatcgaggaa gatgtttctt
     2821 tagataattt cacgatgacc tcagatggac aaggaagctt gccttatttt gatgaaaaca
     2881 atcatttcct agggttagga gtaggaagtg ccaaggcatt gctagtaggg atcaaagaag
     2941 ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg agctataacc agtaatccag
     3001 cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat tggtgctgat gcagatctgt
     3061 gtatcttgga taaagaaacg cttgatattg atacagttat cgctaaagga gaaataatgg
     3121 tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa aagtttttaa tcaacagctc
     3181 taactatctt ttttagaaaa cgagcgttct gtttgtctgt atctgttgtt gatgttaccc
     3241 tgaaggtaga tcaataaaca aactaagagg aggaagcaca atggcagatt taaaaggacg
     3301 ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa gaagcatacg gcaaagcaac
     3361 agacgacaag agcaaagaaa cagaagggaa agcacaatca ctaggtgctg acatcaaaga
     3421 aaaagctcga aatttaggcg acgaagtcaa agacaagttt tctcgaaaag acgaaaaaaa
     3481 ataaaaacca cttattacta attgaatcaa gcaaataaaa aatccgaacc atatctgcga
     3541 gacagatatg gttcggattt tttatttgat cattatcctg ttttttaaac ttcgattttt
     3601 tgaaattttt tattttttag ttcaaagact tcatcacagc tttttgctac ttcaatctca
     3661 tgagtcacga tgataataca gcgattttgt tcatgggcga tttcttggaa aagtttgacg
     3721 atatcttgtg aagttgtttc atctagattt ccagtaggct catccgctac gattagttga
     3781 tgttcacagc agatagcacg aacgatagcg actcgctgct gctgacctcc cgaaagtttg
     3841 gtcacctgtt tttttgctaa tatttcatca attcctacac gagccagtcg atctaaagca
     3901 acttgtcgat aattctcttg cttggtctta gtgatttcca ttgctgctat gacgttgtct
     3961 agagccgata aataaggaag caagttgtaa gcttggaaaa taatcgagac atcattttta
     4021 cgataacttc ttaacccgat tttgttcagt gaacgatctt gatagtatac ctttccttct
     4081 tttggtgcat ctagtccaga aataagcgaa agaaaagtcg tttttccaga accacttgct
     4141 cctaaaatag cgtacatttt tccagcttcg aaattcagtt ctacatcttt ataaagcgca
     4201 tcttgcggat cttgatacca aaatcccaat tgttctgtcc gtaacatcaa attcctcctt
     4261 tagtttaata ggatttttct tgggtttaat cttaatatcc ctatagaagc taaaaagaca
     4321 gaaaacagac tgattccaaa tgctactccg gctaatagtc ccaattgtgc aggtttgaca
     4381 ttgatttcca attcgctgac ttgatcagaa acagcaaatg gattattcgt ggttccttgt
     4441 tgaccatcgc gttgaccgcc ttgttcttgt ctgttacctg gcatttgtcc accgccagga
     4501 gcttgctggt tttgtgtcgt ggtttctgtc tgttggtcaa gtagctgctg tcccacggca
     4561 ttcgcgacga cgtttccgct aagcgaagca actcctaatg caagaatcat acaaatagcc
     4621 acttcggtaa acaattggag aatgatttta cttctggatt ctcctaaaga tagcaagacg
     4681 cctaactcat gccgacgctc ccgaatggta atcataatga tcagagtaag tatgataata
     4741 ccggctacag ccactagtaa aacaacattt ttagaaaaac ttgccacgtt gtttaaaggt
     4801 tccaacattg actgatacat ggaatcattt gattgtaagc taaatgtatc tgtgtcgatc
     4861 agcttctcag cttctttgag aaactcatcc attttatctg gatcagatag agtgtaaata
     4921 gcagaatcta tcgtgttatc gtcagatgtc ccatttagtt catttgcaaa agtgtaagaa
     4981 gtgtaaagtg tattcgacgg attcatgaag ttgaatttca tacccatact gccgcttgtt
     5041 tctctggatt cataaattcc tttgatcgtc atttcataag tggtatcttc atcttcagta
     5101 ctcgtgattg tgaaagtatc tccgactgcc aggtcatttg tttctgctag agtagaatca
     5161 atgactacat tgtttgtgcc tttgtcgctg tcatctattc cttcaccatc cgtgattttt
     5221 gctgttccat cagaaaaatt actattttgt gcagttgctg acacaccact gatttgaaag
     5281 tctgcttgag tcatttgcgg attcccgccc attcctccag caggttcacc gcctgcatca
     5341 gtagtttctg aactgtcttc acttgaatca gaactagtga tggcagttat tccatctttt
     5401 gccatagctg aggcggaact ttcaaaggaa taactcttta cattatctaa ttcagctatt
     5461 ttttccgcgt ccgttaagga aactggtgta agagaaaaac tgcctggatc tggacgttct
     5521 tcagtggtag aaccgcttga agaagtttgt tcctgttttt taaatgaagc ttctcgattg
     5581 gcacttaaag tcactgttgc tccaacttct ttttgtgctt gattcgctgc ttcttctgca
     5641 gcgcttcgaa tcgtgaggcc agctagcaca aaaataagaa tggctgtgaa cacggcaaac
     5701 aataacaatg aacgtcccca ttttactttt gtacttttcc atgcacgttt cataaaattc
     5761 atattttgca tccctcctag tagatgcatt tgataataga caacctatct taaagctagc
     5821 ttaaatctta tgctgtttgc acaccatcaa catgaggttc tgtatatata aaaagtcact
     5881 atagttattg caaaaaatgt ggtaaaatga aaaaagtatg tttcaaaccg acgaagaaga
     5941 aatgaaagag gaatagcaaa agaaatccta gatgctacag agggattttt acgtgcatag
     6001 ttgttctaac aattgctgat actagcgatt tagtttattt gttgtataat aatggggaac
     6061 agctgaatac ttatataagt attctggcta tagcgtttcg caaaagatcc attttgttaa
     6121 tgaagtatat ccaaaataaa atgcccatcc aactgtcaga acgcggatgt gctggtaatt
     6181 ttcctgctga ttctccataa gttgagaaat agtgattttg catatgcttc attggaatgg
     6241 ctcatatatt tagataaaga agaaaatcga catctttaaa cttggatgtc ataagaagag
     6301 gtgaagtttg tgagtaccat taaaattatc ccattaggcg gcgttcgtga aaatggaaaa
     6361 aacatgtatg tcgcagaagt gggcgaggat atttttattt tagattgtgg gctaaaatat
     6421 cctgaaaatg agttgttggg gatcgacgtc gtaatccctg attttacgta tttagaagaa
     6481 aacattgatc gtgtagctgg tgtcttttta acacatggac atgcagatgc gatcggggca
     6541 cttccttact tgttgtcaaa aatttctgta ccagtattcg ggacagagtt gacgatcgag
     6601 ttagcaaaat tgaatgtcag tcgtaatgaa gcagccaaag gatttaagga tttccatgtg
     6661 gtagatgagc atacagaaat agactttggt gaaacagtag tgagcttttt ccgtaccact
     6721 catacgatac cagattcagt ggggatcaat ctgaaaacaa aagaaggtag tatcgtttat
     6781 acgggagatt ttaaatttga ccaaacggct atcccaatgt atcaaacaga ttatgcacga
     6841 ttggcggaaa tcggcaaaga aggagtctta gccttactaa gtgaatcggc aaatgcagaa
     6901 aatcctcagc ctgtagcttc tgaagcccaa atagcagatg aagtatttga tacgatcaaa
     6961 tattgggaag ggcgtatcat cgtcgcttgt gtagcaagta atttgcaacg agtgcaacaa
     7021 gtattggatg cagcagaaaa agcacaacga aaagttgtct tgactggtca agatttcgaa
     7081 cgaattattc gcacagcgat gcgtttagaa aaacttcaat taccaagtga agatctgctt
     7141 gttacgttga aagacatgaa aaactatgat ccagaagaac tgatcatttt agaaactggc
     7201 cgaatgggtg aacccatcaa gtctctgcaa aaaatggcaa acggtacaca ccgtaatgtc
     7261 aagattgaag aaggagattt ggtttatatc actactacgc caagcatcgc gatggaaacc
     7321 atcgtggcga aaacggaaga tatcatttat cgagctggtg gaacagtgaa gttgatctct
     7381 gagaatatgc gcgtttctgg ccatgccaat cctaatgact tgcaattaat gatcaatttg
     7441 atgaaaccaa catattttgt gccagttcaa ggagagtatc gtgaattagc tgctcatgca
     7501 gatttggctc atgcagtagg gatgccatac aaaaatatct atattactgg acgcggagac
     7561 gtgctggaat ataagaacaa gcgtatgagt gttgcaggaa ctacaagtgc agaaaatgta
     7621 atgattgatg gattaggcat aggtgatatc ggaaacattg ttttacgtga tcgaaaaatc
     7681 ttgtcagaag acgggatttt cgtagcggtt gtcactatca gtcggcgaga aaagaaaatc
     7741 atttcaaaac ctcaaattac ttcacgggga ttcgtttacg taaaagctag ccgtgatctg
     7801 atgaaagaaa gcagcaatat gattgaaaca atcgttgaga aacatttaga aagcaatgat
     7861 tttgagtgga gcaaattaaa gcaagacatc cgagatcaat taggccgtta cttatttgaa
     7921 caaacaaaac gccgcccagt gatcttgcca gtcattatgg aagctactca acgtcgaaat
     7981 aaaaaataaa caaaaagagt aagatgaacc tctctataga gtgctcgtct tgctcttttt
     8041 ttgcgaaaaa caaataattt ttgaatacag catccctcgc tttataataa acgatagaat
     8101 aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac gttatttaga agaacaaaag
     8161 atcagctatc aaccacttga ttttagtcat gtggtagaag aatctttcac agaagaacta
     8221 caaaaaagag ggattgatcc agctttgata tgtaaaactc tcgtagtgaa aggaaacaaa
     8281 actggggtga tcatcgcatt gattcctttg aacgatcact tagattacaa aaaaacgaga
     8341 caagtgacga aagatcgtaa agttggtttt cctggaatgg attttgtatt aatgcataca
     8401 ggatatcccc acggagcaaa cacccctatt ggaataaaac tcgcacatcc tgattattgt
     8461 tttttagcag acagttcttt aaaagaaaaa gaagaagtaa ttgtgtcaag cggtgaaatc
     8521 gggagaagcc ttcggataaa agtagccgat ttagaaaaga tcgtccatcc tatttacgct
     8581 gatttaatta gttaagggac gagaagagaa gctaagcaga gcagagacct caaacttgag
     8641 attatatctg cttggtactt ggtgcatcct gtattatctg ttacaattgc tttgtacata
     8701 ctgtactatc aatccaatga aaatatattt agcagctata ttttttcatt gatgtgcaag
     8761 tgatgaaatc gtttttaata aaaaagatac agagaaaacg cgaggtgtaa aatatgaaaa
     8821 ttacgtatca tggacattcc tgtttaacaa ttgaaatgga aaatggccaa cgcgtgatga
     8881 ttgacccgtt tattacagga aatccattga cagacttaac tgcagaagaa gtaaagacag
     8941 attggttgct gattactcat ggtcatagtg accacatagg agatatgatt cctattgcaa
     9001 aagaaaatca agccacaatt atcagtattg ttgagattgc tgattatgcg aacagcagag
     9061 gagtagattc attcggtatg aatatcggtg gaaaacatgc gtttccattc ggcgtggtaa
     9121 aattcgtaca tgcacagcat agttcaagct atgaagtaga cggtatcgtt cagtatatgg
     9181 gcgagcctag cggaattatt attcaagcag aaggaaaaac gatttatcat gcaggggata
     9241 ctgcttattt cagtgatcta ggattgcttg cagaagaatt cgatattgat gtcgctttct
     9301 tgccgattgg cgataactat acgatggggc cagaagatgc agcaaaggct gcgcaaacaa
     9361 ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt ccctgtcatt gaacaagatc
     9421 ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa aataatgagc gtctcggaaa
     9481 cgctggaagt gtaggcagaa aatggcgaca aaacatgatt tgattttaga atatattgaa
     9541 agtttacctg ttggcaatcg aatttctgta agaagcatcg caaaagaatt gaatgtcagt
     9601 gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg tcggactagt ttcaacaatt
     9661 caacgagtag ggaccatccg tatcgaacgc aaattaaaaa aacatattga acgtttgaca
     9721 tttggcgagg tcgtcaggat cattgaaggg gacgttttag gcggggcggc aggattagac
     9781 aaggtcttga ataaattcgt catcggcgcg atgactaaac atgccatgga acgctatatt
     9841 acgccaggat cactaatgat cgtcggcaac cgggcggaag tccaaaaatt agcattggaa
     9901 gatggcgccg cagtattgat cacaggagga ttcaatacga ctgctgagat tgcaaagcta
     9961 gctgatgatc ttgaaatgcc gatcttacgc acctcttatg atacatttac agtcgcaacc
    10021 atgatcaacc gagcgttaag cgaccaattg atcaaaaaag atatcatgct tgtcagtgat
    10081 atctatatgc cagctgaaaa gactcattat ttgcagcaga cagatacgat ttctgattat
    10141 cagcgtttgt cagaagaaac gcagcattcg cgttttccag tcgtcaaccg ccatcatcgt
    10201 ttgatgggga ttgttacggc aaaagatgtt ttagggaaat cgccaaacca gatcatcgat
    10261 cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa tgagtattgc ctctgttagc
    10321 catcaaatga tctgggatgg gctagaagtc atgccggtcg tttccgatga cttgacattg
    10381 gaaggcttga ttacccggca agatgtaatg aaagcgatgc agcttgtcca gcgacaaccg
    10441 cagattgcag atacgatttc ggatcagata tctggaaata tccatacgat tgatactgac
    10501 cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg tagtagcacc acaaatggtc
    10561 aatggcgttg gaaccatttc ttttggcgta cttagcgaaa tcatttctga tgtcgcacaa
    10621 aaaacaatgg tgatgaacca aaaacgaaat atcttgattg aacaagtgaa cctacattat
    10681 ttgcggctga tccaattgga aagcgaatta gacattcgtc cacgtgtatt ggaaatcggc
    10741 agacgttccg ctaaactgga tatcgaagtg tttattgaaa acaccatcgt ttccaaagca
    10801 atcgttgttt gtcaagtaat ggaacgttct tagaaagcag gagtgaaatg acagtacagc
    10861 aagaaatagt agatacaata aaaaaatacg ataaaatcat tatccacagg catttgcgcc
    10921 ctgatcctga tgcattagga tcacaagtag gattagccga actattacgg gaaagtttcc
    10981 cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg gcttcgcttt ttagcagaaa
    11041 tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt catcgtgact gatacagcta
    11101 attcacccag aatcagtgat caaagatatc agttggcaga aaaactgatc aaaatcgacc
    11161 atcatcccaa tgatgaccct tatggagatc ttgtgtgggt aaatacaaaa gcaagtagct
    11221 gtagtgagat gattgcagaa tttgctttga tgtttcctga atctttaaag atgaacgtta
    11281 aggctgcacg gttactttat gcaggaatcg tcggagatac agggcgcttt ttgtatccct
    11341 ccacgacgtc ccgtacatta gagattgctt cgatattgag aaattatcca tttgatgcaa
    11401 gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt cgctaaactt tccggttatc
    11461 tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag aataatcctt ccgcaagaaa
    11521 ttcttgataa atatggaatc gatgattcag agacagcttc gatcgtttca cttcctggag
    11581 tgattgacga agtgctggct tgggggattt ttgttcagca gccttcaggg tattaccgcg
    11641 tacgtttacg ttctaagggg ccgatcatca atgaattggc taaacgccat catggaggag
    11701 gccatccatt agcaagtggt gcgaatgcaa aagatttagc agaagtagag gaaatctata
    11761 aagagatcca agatatatgc caatcgtata agagcagaaa aacagaatga tacttcgttc
    11821 gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt ttcaagatct tctttttccg
    11881 ttacaatagt gctcactaaa gattttaaag gagaaagaag atgacattac caaattgccc
    11941 agaatgtggc tcagagtaca catatgaaga tcgaggcttg ttgatttgcc cagaatgcgg
    12001 gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa gacggattag tggtattaga
    12061 tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca gtgattaaag acttgaaagt
    12121 aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc aagaacatcc gtttagtaga
    12181 aggcgatcat aatatcgatt gcaaggtaga aggatttgga ccgatgaaac tgaaatcgga
    12241 atttgtgaaa aagaattaaa gaatattcaa ggtcttttca cattcttttc ctactactaa
    12301 tgataaaata aagtaaacga aaaggagtga aacgaaaatg tatggttatg gaatgtatgg
    12361 ttttttagat ccgacatttc ttttggtcat tcttggatta gttatttccg gaattgcgtc
    12421 agcgtacgtc aatagtactt tccgaaaata cgatcaagtg agaagcaaaa acaatgtgac
    12481 tggtacacaa gcagcacaat atatattaca aagtcaacag ataaataatg tcggtgttca
    12541 acaaattgcc ggagatctca cagataacta caattcaggt aataagatgt tgagtttgtc
    12601 tcaagcaact gcacagtcta cttcagttgc tgcgataggt gtagcagctc atgaatgcgg
    12661 acatgcggta caagatgctt cgggttatgt cccattgaag attcgtgcag cacttgttcc
    12721 agcagctaac tttggttcga tgatttcgtt ccccttgatt atggtaggtg tattgcttag
    12781 ctggaactct acgttaatca atatcgggat tttcgctttt tctttagcat tacttttcca
    12841 attggtgaca ttaccagtcg aatttaacgc gtctcggcga gcaatccaaa ttttgagtga
    12901 aggcggacta ctgacggaag aagaagtacc gatggcaaga cacgtattat ttgcagcagc
    12961 actgacttat gttgcagcag cgctttcgac attcctgcag ctgttgcgct tgatcttatt
    13021 gttcggcggc aaccgaagag attaaaataa agaagttgtg aaatcagcgc tttgatctga
    13081 gctaataccg agagttagct gattgaatac catttatttg gaataa
//
LOCUS       DASJUF010000070        12968 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJUF010000070 DASJUF010000000
VERSION     DASJUF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.070"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            78..686
                     /locus_tag="U4B62_001905"
                     /pseudo
     CDS             78..686
                     /locus_tag="U4B62_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="U4B62_001906"
     CDS             706..1734
                     /locus_tag="U4B62_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2142060.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="U4B62_001907"
     CDS             1731..2489
                     /locus_tag="U4B62_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2142061.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="U4B62_001908"
     CDS             complement(2575..3315)
                     /locus_tag="U4B62_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2142062.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="U4B62_001909"
     CDS             3376..4629
                     /locus_tag="U4B62_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2142063.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4930..5178
                     /locus_tag="U4B62_001910"
                     /pseudo
     CDS             4930..5178
                     /locus_tag="U4B62_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="U4B62_001911"
     CDS             5300..5626
                     /locus_tag="U4B62_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2142064.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="U4B62_001912"
     CDS             5694..6407
                     /locus_tag="U4B62_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN2142065.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /locus_tag="U4B62_001913"
                     /pseudo
     CDS             6717..7569
                     /locus_tag="U4B62_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7690..9555
                     /locus_tag="U4B62_001914"
     CDS             7690..9555
                     /locus_tag="U4B62_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2142066.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9603..11012
                     /locus_tag="U4B62_001915"
     CDS             9603..11012
                     /locus_tag="U4B62_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN2142067.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11120..11821
                     /locus_tag="U4B62_001916"
     CDS             11120..11821
                     /locus_tag="U4B62_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2142068.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11980..12459
                     /locus_tag="U4B62_001917"
                     /pseudo
     CDS             11980..12459
                     /locus_tag="U4B62_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         4342 a         2182 c         2746 g         3698 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 ctttttta
//
LOCUS       DASJUF010000071        12858 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJUF010000071 DASJUF010000000
VERSION     DASJUF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.071"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(130..744)
                     /locus_tag="U4B62_001918"
     CDS             complement(130..744)
                     /locus_tag="U4B62_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2142069.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1350..1727)
                     /locus_tag="U4B62_001919"
     CDS             complement(1350..1727)
                     /locus_tag="U4B62_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142070.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2027..2908)
                     /gene="galU"
                     /locus_tag="U4B62_001920"
     CDS             complement(2027..2908)
                     /gene="galU"
                     /locus_tag="U4B62_001920"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2142071.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(2951..3970)
                     /locus_tag="U4B62_001921"
     CDS             complement(2951..3970)
                     /locus_tag="U4B62_001921"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2142072.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4603..5607)
                     /locus_tag="U4B62_001922"
     CDS             complement(4603..5607)
                     /locus_tag="U4B62_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2142073.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5620..6669)
                     /locus_tag="U4B62_001923"
     CDS             complement(5620..6669)
                     /locus_tag="U4B62_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2142074.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6686..7507)
                     /locus_tag="U4B62_001924"
     CDS             complement(6686..7507)
                     /locus_tag="U4B62_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2142075.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7494..8273)
                     /locus_tag="U4B62_001925"
     CDS             complement(7494..8273)
                     /locus_tag="U4B62_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2142076.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8290..8760)
                     /locus_tag="U4B62_001926"
     CDS             complement(8290..8760)
                     /locus_tag="U4B62_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2142077.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8772..9191)
                     /locus_tag="U4B62_001927"
     CDS             complement(8772..9191)
                     /locus_tag="U4B62_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN2142078.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9272..12049)
                     /locus_tag="U4B62_001928"
     CDS             complement(9272..12049)
                     /locus_tag="U4B62_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2142079.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12240..12464
                     /locus_tag="U4B62_001929"
     CDS             12240..12464
                     /locus_tag="U4B62_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142080.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3750 a         2694 c         2108 g         4306 t
ORIGIN      
        1 tcttaaaata actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc
       61 tttttttcaa acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca
      121 tgtggataac tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac
      181 ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt
      241 tggcaaagtc aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa
      301 ttcaaataat cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc
      361 agtaacaatg attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc
      421 cgggtttctt ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg
      481 atcaataatt gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc
      541 aatcaatata cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact
      601 tagtgtgcgg ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca
      661 agtgccagct tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata
      721 atagtcaaaa taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag
      781 aaacagctgc ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt
      841 ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat
      901 agcaaataaa acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc
      961 tagattaggt acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc
     1021 agggtgtatg tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag
     1081 ccttttttta ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc
     1141 aagcaagaaa atacaaaaaa acattggtat gatttatata tatataaata acataaaaaa
     1201 aggaatggct tatcccgata caacaagcat tcctttaaaa aagattcttt tatataagtg
     1261 aaaaaagctt ttttccacca attaaataaa tctctcatct caatagttta tccattattt
     1321 tatctttttt tgcgtatacc tactaaatat taccgtcttc ttctcgttct tctactttgt
     1381 tcgagagaat ctgtactaga aaaagtagtt gtatgtctac cttttctgcc tatccgagac
     1441 tttcttttca tatatgggat ataaatgatt agatacagta cagcaaaaaa taaagcaaat
     1501 acgcaagctt cccaactacc taacgccaat tcaaccatca aggtagcaac cccaacggat
     1561 gctgcatacc agataaaaat cagaaacgtt tctttttttc tgaccatgcg aaacatgata
     1621 aatagactaa taagcaatag tggcaacaca aatatccaat tgcttccttc catcagccac
     1681 ttgagtatat caagcagcga ctgtgcaaca gcgactaatt ttcccatagt gaacacacac
     1741 ctttttttat aattaaaaaa ttcgcctttt tattctacta tgaatcttct ggcttggctt
     1801 tcatttctct tatgtaatca ttttctttta tacaacttaa aaaaaaatta aaccattagc
     1861 ataatatttt ctgaatctat tcacgaaagt ctttttctat ctttcgtttt ccacgactca
     1921 actgatctag agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca agctgccgct
     1981 ttaataacaa atagcctttc attctcagca ggccacttat ttccagttaa ctttctgttc
     2041 ttagcttttt actcaactgg ataatatatg cacttaatga agtcccaatt tccgaatgtt
     2101 gaagtccata ttcaatattt gtcttcatat atcctaactt atcgcctacg tcgtatcttt
     2161 ttcctttaaa ctctctcgca tagaccgcct ctgtctggtt aagagtctga agtgcatccg
     2221 ttagctgtat ctcgtttcct gctcctggtt cttgagtttc taatatctca aatattttag
     2281 gagtcagtag ataacgtccg ataatggcta aattacttgg tgcttttccc atttcaggtt
     2341 tttctacaaa cccttttact ctgcaacgac ccttttcgct atctgtttta ggatcaataa
     2401 ttccgtattt agatgtttca ttttctggta cacgcatgac ggcaacagcg gaagcttgtg
     2461 tttcttcata atccatcatc aattgttgag tcaatgggat atcatctttc atcaaatcat
     2521 cccccagcat gacgacaaac ggttcatttc ctataaatgc ttttgcttga agcacggcat
     2581 cccccagtcc tcttggatga gattgacgga tgaagtgaag attgacttga gtcgtttctt
     2641 caactaaagc gagtaattct gttttttcct tttcccgcaa attgttttcc aattcgatat
     2701 ttgcatcaaa gtgatcttca atgggtcttt tttcttttcc cgttacgatc aagatgtctt
     2761 cgattcccga atccaatgct tcttccacaa taaactggat cgttggttta tccacgattg
     2821 gaagcatttc ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg
     2881 caggaatgac tgcttttttg accttcactt gctgttcctc ctaaacgacg attaactatt
     2941 gatcatctat ttaacgaaga gaaaattcat tctccatttt tccgtcacgt aacatgattt
     3001 cttttgctgc ttttttgata tcttctccat tatacaaaac attatagatt gtttgagtaa
     3061 ttggcatctc aacacctagc tgttgagcta gctctactgc tgcttttgtc gttgccacac
     3121 cctcaacgac cattcccatg ttttctagaa cttcttccag cttatgtccc tgtcctaaca
     3181 agttaccggc acgccaattg cgagaatgaa cacttgtaca agtaacgatc aagtccccca
     3241 caccactcaa accgataaat gtcagaggat tggcacccat cgcgacacca agacggctga
     3301 tttctgccaa tcctcgagtc atgattgctg cttttgcatc atctccgaat cctaatccat
     3361 ggatagcgcc ggcaccaatt gcaatgatat ttttcagtgc tgcaccagtt tctacgccaa
     3421 tcacatcatt atttgtataa atcctgaaat aatcattcat gaacagtttt tgtacatagg
     3481 ctgctgcttt ttgatcaata cttgcagctg taattgttgt gatatcatgt actgccactt
     3541 cctcagcatg gctaggacca gataatacaa caattgcctg tcttttttct tctggaattt
     3601 cttcagccaa aacttctgag attcgtttat gcgtatcttg ttctagtcct ttacttgcat
     3661 gtataatgat tggcttagta ttcattttac caataagttc ctgtgcgact gaacgaatcg
     3721 cttttgttgg aacaacaaat aaaatagcat ctgcgttatc cactgcttct gccaattctt
     3781 tatatccgat aatcgattct gggatcgtca aatcaggtaa aaaatgctga tttgtatgat
     3841 acttattgat ttcatcaatc tgttcaggta catttcccca aatacggaca tcatgcccgt
     3901 tttctgccaa tgtttgtgct aaagcagtgc cccaagatcc aggaccaaga actgcgattt
     3961 tttgtttcat ggatattcct ccaaataaat aattgataat ttccattata gtatacattt
     4021 cttaaagttg ggactaattt ccattgtttt taagtgtttg tttacttctt ttatcgtttt
     4081 tacttgaaat aatcaatgat atgttacaat agtctacatt ttatttttac actcgttatg
     4141 ctttgcttgt tcctaagagg aagcagtttt gacaatgtta ctaccagcat tactattagg
     4201 aggttaggaa acttctccta gtgagtattg atttaaaata cataagagac agattgaaca
     4261 tttctaaatc tgtcaggtcc tgcaacggac agattgtctc tgttgacggt tgacatagga
     4321 acttttgttt tagtcaacat tctggataca aaacgctatg taaactggcg caactcgttg
     4381 tacttggact tttactacgg atccatgcaa caactaaata gtactattgc ataacaaaaa
     4441 atggttggca cccacatgtc ccattaaaca gattataagt tgaactgtag ataatgcaaa
     4501 aaatgtgttt tataacgtaa ttgatgccaa tattttaaaa aaataaaggg agcagttatc
     4561 gctgctctct ttatttttta tattcttttt atccagtcta tcctaataat caaactgacg
     4621 atagtagcgg cgtatctcta atggatggca gttcaactgc tccacatgag catcgatgcg
     4681 ttgtgtcact acatgcatca ataagaaaga caaaacacgt ccgcgatatt tttcactgat
     4741 accttctact ggatagtctt ttgtgtctat caatgtataa ttgctgcaaa tttttggcaa
     4801 aagtttagcc acacgttcag ataacactct ctgttcatct tcgcctaaaa ataatgtgac
     4861 tggggttgtt tcttctacga tctctaacgt cccgtgaaga aactcggcag catggataga
     4921 tttcgatgga agccagcttt gttcttccca gtaacacatg gcatatgaat aaacagctcc
     4981 ccattgattc cctgctccaa taaaataatg catactatca cgtcgatgtt tttctgcaaa
     5041 tgataagcca aatgcatctg cttttttctc tgcttccact aaccctgttg gtaaataacg
     5101 ttcgagctgt tcatagtatt ctgaataagc ttcgaattct ccattcaaat acatcaatcg
     5161 atctgctgcc ataaaaaatt tgatttgttc tgttccagga gctggataag tgaccacaaa
     5221 atcgcatagc tgagacaact tactgttcgc tttatcgata aatccaatca gtgtcgctcc
     5281 tactttcttg atttgttcta ctgctttcac tacttcttga gttgtaccgg taacagagga
     5341 caaaataacg atagaatctt ttgttaatct tttgtttcca gtagtgtaat attctgcagc
     5401 atgctgtaca aagacaggca agttgctttt cccattcata taagtgacag cttgcatcga
     5461 ggaagcatac gttccaccga ttcctagata atagacagcg tcaaattttt ccgcataaag
     5521 tttatcgatg atttcttcta cttgcggacg taaagctagt gcgcctttta ttgctttgat
     5581 ttgttcttct tcattgaatt tcagcatgat ttccacctct tataatttac ttttcaagac
     5641 tgtatcaaag tcttcaaaaa tctttttgtt caagtcaata cctttagcta ctgcggtttt
     5701 ataagcgaat agttgaaacg gaacgacaag cgctaataca gacagatatt catcacattc
     5761 tattgctaat ccaagtgttt tctcgtttct tgcttttttt gtggtaatgg tgagagtctc
     5821 tccaacgtac cgactcatat atctttgcaa cttttgcgaa cgttccttat ttacactttc
     5881 cccttcaata aagaatagca aatgcgaagc atctgcctcc aggtatgggc cgtgcatata
     5941 agcttctaac tcaaatcctt gtgacggaac gcggattgtt tcagtcaatt tcgtctctgc
     6001 ttctttagct gttccccagt ttggaccata tccaatggtg acgaaacgct gtgctaaacg
     6061 aaataaactt tgatatccat caaaaaattc ttccgttttt tgaataatcg ctggtaaatg
     6121 gttgattatt ttgcgcaact gttgcatgta gtcttgttct atttttttgc tgattttatt
     6181 tttacttatt ccaattccta tccctaagag tagtaactgt aaaacagtcg cagaatagcc
     6241 ttttgtaacg aagcctactg attctacccc ggcacctaaa tcaataattt gtgcagcttc
     6301 taaagcaagt ggactttgca cgtcatttgt gatagcaaca acaggtaacc cttgtttttg
     6361 tatcattttt actgcttcaa cagtagaagc acttttcccg ctttgtgaca cagcaatcac
     6421 agtatcgatt gaaggagaaa gttttccata attgaaaaaa taaaaaggtt cttgaatatc
     6481 gacagtaaga tcaccataag attccaatgc tggcttcgct gctaaacaag cgttataaga
     6541 tgaacccgtt gctaagatca gtaaattcac tgtttttttc atatgttctc ttgtcgaaaa
     6601 atcattcttt ttcaagatcg tacttagtac ttccttttct tcatagatat aatcttgcat
     6661 cgtaaacatt ttctctgctc cttttttaaa aaatccctat ccacgaacca aaaattccaa
     6721 agaaagctag tccgatcaag atcgttaatg gtttcacttc tttcttcaat agataaaaca
     6781 ctatgccaaa tgctccaaga cataagatat ttggaacgat atcatcaaaa atactttgaa
     6841 cagtcacagc attttctcct gaaccgattt ttaaaggaat attgatggtg atcatcgtgg
     6901 cagtcattgc tccaacgacc atcagtccaa taatagacgc gcctaatgtc aaactttcca
     6961 tcataccgtt tgcctggatc tttttcaaaa aacccgttcc taagcgatat ccccaacctg
     7021 ttgctagata gcgaaacaga atgtgcggaa tattgaaaat caaaatgaac aagatagggc
     7081 ctaaaatatt tccttgcatg gctaaagagg tacccacacc tgtggcaatc aatcgcaaag
     7141 ttccccaaaa gaaagaatct cctaaccctg caagaggtcc catcaaagat gttttgatac
     7201 tatcgatcgc agtcacatca aattcaggat catccttatt ttcttcctcc attgcagccg
     7261 tgattcctaa aatcagtgta acgatatgtg gggtcgtgtt aaaaaactct agatgccgtt
     7321 tcaaagctgc tgccatttct tctttcgact gatataactt tttcaaaatc ggaatcattg
     7381 cataaacata agccatattc atctgacgtt cataattcca agaaaactcc atttgaaaag
     7441 aacgccaaaa tacgtgattt agctcttttt tcgttatttt gctgtttttg atctcagaat
     7501 tcttcatctt cttctacctc ccctgcagtt gtttgaagtg gtagttgacg gacattcata
     7561 atattgacta ccacaactgc taaaatcgca ccaaaaatag ctactccggt cacaggaatc
     7621 tctaagtaag aagctatagc aaaacctaag aaaaaatatg gtgcaacttg tttgttgatc
     7681 agcaaacgag caagcatcgc aaaaccaaga gctggaatca accctgtagc tactgacaac
     7741 cccttttgaa cgaagtcagg aatcatattt aatagatttc cgatcgtatt gcttcccacc
     7801 atataagaaa ccattaccac aacaccaagc atcaaggaaa gaccgatacc ggcaagcaag
     7861 tgcatatgct cgactccttt ataatttcct tcttctgcat aattgtctgc tttatggttc
     7921 ataattggaa tcaaaagccc taagtaaata tttttcaata tcaatgtcaa cgtagcaatc
     7981 ggcaaaccta gtaaaagcgc ggtttctgta ccggctccag ctgtaatagc aaaagccgtc
     8041 cctaaaatcc caccggtcac aacatcaggt ggaatcgaag cacctactga gaacgatccg
     8101 ataaatgcta actctaaagt tgcgcccata atgatcccag tttttacatc acctaacacg
     8161 attcctgtaa acagccccgt cacaatcgga cgagaaagca acgatgtccc tagtgcatat
     8221 tctgattgtg cgacaaatgc aacaattcct aataagactg cttgtacaat cattgaggtt
     8281 cttcctttct tacaaaactt ctcttacatc cactttttta tctgctggta cttgacggat
     8341 ttctacttct atttgtttgt caattaatcg tttcaagcgt tcctcctctt ctggtaaaat
     8401 atttaccgct tttccgatgc tgcgagtacc aggttttgcc ttgatccctc ctaagttgat
     8461 ggttttgatt tctggatacg cttctgccag cttatatgca tcctcgatcg attccactac
     8521 gatgaataac ttatatttat ctgtcacccc gcttttcaac gcggcaatcg agtcatcaat
     8581 tgatttgatc actagtttga ctccttgtgg cttggctaat ttgatcgttg tctttcgtat
     8641 ctcattcgta ggtacatcat cattagcaat caaaatacag tctgcgccta ttgtttgtgt
     8701 ccatgaaaaa gctacttgtc cgtgtaataa ccgatggtcg actcttgtca atacaatcat
     8761 ttttcttcct cctaaaattc gtcttcttct attttctgat tgagccgctc gttacaaaac
     8821 tggatcattt tttttgaatc agttagcacc tgctggatca aaacagttgt ctctacttca
     8881 gcgtccaacc gagatactag ctcaatcaat agtggcaaat tcaatccggc aacgagatag
     8941 acatgatccc gattgatata ttgtaagaat tcgttgttga cacttccgcc aaaaagatcc
     9001 gtcagaacaa tgatttcttt atctttgtac ctcataaaaa gctgccttac tgtttccgct
     9061 aaatcaaaat tctcatcagt atagcaatcg aatacagtaa tagaatgatt tttcccatag
     9121 atcagctcta atgaatctaa tatccctgaa gcaaattttc catgggaagc taagataatt
     9181 tctctatcca ctttttcatc tcctcttttc acttacacat actaaaaaag caagttgcat
     9241 gccaaaatta gtggcaacaa cttgcttatg ttcaaaattc ttcttctatc gagagaaact
     9301 ctgtatcttc actcaaaata tcatagatat aggcaatctc agaattgggg atcatgacac
     9361 tataatgctc ttcaatgaca ctaaacgcag atttaatagc agataaccct tctttttgac
     9421 actgcttcca tttctctaat ccttgatagg tttcgattgg catttgtcgg atcaatcgtt
     9481 caatgagaca gctcacatgg acatacaacg ctaatttttt gggattgctg atgcttcgat
     9541 tcaactgcac ctctaattct ctcataaaat gatcgatttc tcccatcacc ttctctgtat
     9601 ctagaatagt cacggaatcc agtacttttt ccaatgaaaa attacgaatg atgcgctgat
     9661 tgaatatttc cttctctgaa gcattcatag tagtgtccaa ccattcagct agtacagaca
     9721 tcttttcccc cgagatgagg tcttctaatg aaagatagtc gatattggtg atatcaggat
     9781 ctgtggtgcc aatcattcct acgacttcgt atatggaaaa taatgggtca ctgtttttct
     9841 tatcttttaa ttgctgatat tcatatggga tgattttcaa ctgacaagcc gtaggtaagc
     9901 ttttttctag cagttcactt aagtggaccg ccgtaccgat tccagtgaag caagtagtaa
     9961 tgagtgcttt gggtttgttt tcctctggat agagcagtcg ccattctggc tggatctgtc
    10021 gaacagtctc ttctgctatc tcttctaatg atacattttt ttgcaggcat tcccctactg
    10081 tgattgctaa aggtgtcgtc acattattca aaatcaaaag cggtacggat aactgttttt
    10141 taaaaaactg atggatttct ttcagagatc ccatgtctac taatatgatc aatccatttg
    10201 aaacatcgct gttttcgcaa taatcaagga tttctgaagc aatctgctgt ggtgtgacat
    10261 ctagtggcat atcaaatgat tcaaatagat cctcttgtaa taaacgattt actacatttg
    10321 ccatactgct agcagttgca taaccgtgag cagcaatcat cgcttttggc agtcgttttt
    10381 cttttgacca tttcgctttt tttagataga tcgtcagtaa aatccgatcc atacttgtgt
    10441 cttcaatatc taatttgatc ttacttaatt ccagaattcg ttggacatag tgataactag
    10501 atggataaga ctgagaaact tgttgttcta attctttgat tagttggata atatcatgtt
    10561 cttccggtaa ccatttgata gtgcttcgct gatagagata gtaactcaag gcatagacac
    10621 tgtttccatc gaatttgatt tgataggcag tttccatttg tcgaaaggtc tctcggatag
    10681 attgtgtcca ataaaccagc agctcgtgtt tttcttgtct actggtctca aacagcaaaa
    10741 aatcaaataa ttgaatcact tcttgcttta attttccttc gcaagaatat agcagacagt
    10801 tatttttctt gaaatcaatt aggatttgct caaaactttt aacgattcgc tgctgttcgg
    10861 gctgttgctt ctccattaat tgctctaagg tctggtcttg cgtaatatag attccttgct
    10921 gtgctgtatt tttagtaata ggctgcgcaa gtagttcttt ttgcaaatga tggatcgtga
    10981 catggatttc aggctgttca cgctgttcag catatgcttt tgctgctgaa actttgacga
    11041 tatttttcag ctctcctatg tttccggata atttctgact tgttaaaagt gacaaagctg
    11101 gtccactgac tgacaaaggg ctccccattt ttctttgttc ttcgagaaaa aaagagtaaa
    11161 tcagttctag ccgttcatta cgactacgct gattaagtgc aggtagttcg attcggatag
    11221 gtattcttcg aatgaaagtt gttaagaaat gactagtaat ctcttcagtc gtagcaaaaa
    11281 agagacgtgt tttgatccgg cgtgacaaat taggttctcc catacgataa atcacacctt
    11341 gatcaagata ggtgaacagt ttttcctgac cttctggtgt taagcgatgt acctcgtcta
    11401 aaaacaagat cccaccatct gctgcttcaa aagccccttt tttattttct tctgcaccag
    11461 tgtacgctcc cttaacatgt ccaaacaaat tgctagtcaa aagttcagga ttatctgcat
    11521 attgtgcaca attaaaagtg atgaatggtg cagaatcttc tagcagatca tgaagcagac
    11581 aatactgata gaccaaatta actaaataac ttttacctgt gccactttcg cctgtaatca
    11641 aaagtggtaa tccgccatct ggatagtaaa gagctgtttt gatttgttcg atactttctt
    11701 tcaaactggc gtcgtatccg atcataaaag agaataagtc ttttggttgc gcgaaaaaag
    11761 gctgttcctc tttgagatca tcgatagaaa aataaaaatt cgtgcgtaac ttaaaaaatt
    11821 gtttttcaaa agcttctttg tggaaataaa tcactggtcg agaattgatt ttgactagta
    11881 gttgctcatc atttaactgg ttcaaataat gactgactgt gtttcttttt accccaaaat
    11941 gatcagctaa cctttgtgct gtaaagacct cgttcatatg ttctaaatcg aaaaaggctg
    12001 tctggttctc taagtaacat aaaatttcct tcttcaacaa cttattcacc cctcctccat
    12061 catagaaaac ggattcattt tgtcaataaa atctttaata aagaattgaa aaatatatct
    12121 tattatagac agcagcctac ttatccctta taatttttat tctaaaaaac gtacagttcc
    12181 ttccagtttt tatactggaa tttcaacata tgaaagaata aggaggctat gactattcaa
    12241 tgaaatcgga taaatataaa aacaggaaac atacttcctg gaaaactttt cgagaaatca
    12301 tcggatctta tctcgctgta gttgtatttt tgcctttgct ttatatggct atgacagaac
    12361 aaactcgttt tattggcgta gatacgcaat atatagccga aacaggattt ttattcatct
    12421 ttatcttgat ttttgcgttc tataaatcct tccatacaaa ataagattca cagatttcaa
    12481 ctttcctgtc tgtttttagc tattacaaat ccttatatgt ggtataacaa caatttattg
    12541 gcataatgag gaaaaacgag ttatgatgct ttcagagagt aaagaggtac tgttgtcacc
    12601 atccttttta ttgattacgt acaccactct tagttttttc ccattgatta cgaaaacaag
    12661 gtagaaacat tgatgttcct accttgtttt ctttttttat tttcagatca aaatctcaag
    12721 tagcttttga cttgatgtcg ggaggttgtc ttcgttccat caaacctgat agaatctgcg
    12781 ccaaaccata gttttctcca ctattattta ttcctctaag atagtaccca caataaacga
    12841 ataaatattt ttctcatt
//
LOCUS       DASJUF010000072        12774 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJUF010000072 DASJUF010000000
VERSION     DASJUF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.072"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..414)
                     /locus_tag="U4B62_001930"
     CDS             complement(<1..414)
                     /locus_tag="U4B62_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2142081.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKD"
     gene            complement(411..1064)
                     /locus_tag="U4B62_001931"
     CDS             complement(411..1064)
                     /locus_tag="U4B62_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2142082.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1352..1872
                     /locus_tag="U4B62_001932"
                     /pseudo
     CDS             1352..1872
                     /locus_tag="U4B62_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1774..2490)
                     /locus_tag="U4B62_001933"
     CDS             complement(1774..2490)
                     /locus_tag="U4B62_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEN2142083.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2540..2749)
                     /locus_tag="U4B62_001934"
     CDS             complement(2540..2749)
                     /locus_tag="U4B62_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142084.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3026..4299
                     /locus_tag="U4B62_001935"
                     /pseudo
     CDS             3026..4299
                     /locus_tag="U4B62_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4406..5437)
                     /locus_tag="U4B62_001936"
     CDS             complement(4406..5437)
                     /locus_tag="U4B62_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142085.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5444..6274)
                     /locus_tag="U4B62_001937"
     CDS             complement(5444..6274)
                     /locus_tag="U4B62_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2142086.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6271..7086)
                     /locus_tag="U4B62_001938"
     CDS             complement(6271..7086)
                     /locus_tag="U4B62_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2142087.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7083..7907)
                     /locus_tag="U4B62_001939"
     CDS             complement(7083..7907)
                     /locus_tag="U4B62_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEN2142088.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7897..8997)
                     /locus_tag="U4B62_001940"
     CDS             complement(7897..8997)
                     /locus_tag="U4B62_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2142089.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9175..9960
                     /locus_tag="U4B62_001941"
     CDS             9175..9960
                     /locus_tag="U4B62_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEN2142090.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9993..10376
                     /locus_tag="U4B62_001942"
     CDS             9993..10376
                     /locus_tag="U4B62_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN2142091.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10452..10583
                     /locus_tag="U4B62_001943"
     CDS             10452..10583
                     /locus_tag="U4B62_001943"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2142092.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10552..10788
                     /locus_tag="U4B62_001944"
     CDS             10552..10788
                     /locus_tag="U4B62_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2142093.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10866..11837)
                     /locus_tag="U4B62_001945"
     CDS             complement(10866..11837)
                     /locus_tag="U4B62_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN2142094.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12489..>12774
                     /locus_tag="U4B62_001946"
     CDS             12489..>12774
                     /locus_tag="U4B62_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2142095.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3825 a         2859 c         2310 g         3780 t
ORIGIN      
        1 atctttcgca tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc
       61 atcattaagc tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt
      121 aacatttata ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac
      181 ttttatttca ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt
      241 tccattcata ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc
      301 caaatatgtt ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc
      361 aagaacagaa aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc
      421 tccaatttat atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta
      481 agttctttta tatatacatc tattactcga tcataaggag tttcttcgct atctttccat
      541 acataatcaa taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt
      601 aatacttcta gttcttttgc atttacatct atttcttcac catttatttt agctgtatag
      661 ctattaaaat taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc
      721 ttaattaaag aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat
      781 ccgtctacta aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag
      841 attagaattg gtaaattgct tttctttcta atttctttca acacttctaa accatttata
      901 aaaggtatct gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct
      961 tctcttccat ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt
     1021 ccctctctta tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa
     1081 gcctatacac tagatattat acagcatatt atggctctat aatatctagt gttggtgacg
     1141 ccttctgttg ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc
     1201 tttagaaaaa aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac
     1261 taacactttt tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac
     1321 caaaacatca ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg
     1381 attaacagat aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg
     1441 cgttcaagcg cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg
     1501 tggatcgatt cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt
     1561 caaagaaaag ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg
     1621 gcagaccttt tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact
     1681 gaagcaggcg attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag
     1741 aagtaccgtc ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag
     1801 ccgctcaaac ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc
     1861 gatgaaccgt tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc
     1921 agtcgccaca tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg
     1981 ttcaggatct tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac
     2041 gtaggcaacc agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta
     2101 atcgattgct cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca
     2161 tgaactatcg taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat
     2221 ttgttctaac aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt
     2281 atgcccgcta aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg
     2341 tgctgcttgg gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg
     2401 tttcaagcac aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt
     2461 cacaaaggaa tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc
     2521 ccgcagttca gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat
     2581 gttcagcatc cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg
     2641 ctgccgtccc aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca
     2701 gtaaataggc ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat
     2761 catcgtccca tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa
     2821 acaagcaaaa gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa
     2881 agtcaaaggt gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat
     2941 cagtcctttt gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca
     3001 caacacaatc aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg
     3061 aatcaaagat ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa
     3121 cagtatcgtt gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca
     3181 ggtgaatcaa catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa
     3241 taagtgtgcg aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg
     3301 ctgtcacacc tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa
     3361 acaagtacat tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc
     3421 taaacattgc ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat
     3481 gacaaacgtg aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt
     3541 gaaaaatcaa gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact
     3601 ggatattctt ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg
     3661 acgtgcacgt ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct
     3721 gataaaagag tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca
     3781 cctcattcgc agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga
     3841 tccaatacaa gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg
     3901 acaggacacg cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt
     3961 aaccgaaagt gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta
     4021 tgcttactat caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca
     4081 gcttgtaaaa acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt
     4141 tataaactat gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat
     4201 agaaaatctg catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga
     4261 accagaattt tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc
     4321 agaaaatcaa ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga
     4381 gccagaaatg tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt
     4441 ccacagggat tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt
     4501 cccgatcgtt caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc
     4561 gttgaatgtt gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag
     4621 gaaccggttc aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag
     4681 cggaaaactc gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga
     4741 taaattccgc aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct
     4801 gagccacccg cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt
     4861 ggtcatgagt cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc
     4921 gctgcagctg ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga
     4981 ctttcggttc agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa
     5041 gaaactggac atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa
     5101 ccttttcctg aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt
     5161 caaaaacagt catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat
     5221 ccttcgtagg cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt
     5281 gttccaatcc cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta
     5341 aagtaaccag ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat
     5401 caaaaaacag attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg
     5461 gcttcttttt cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc
     5521 gataagtcag cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca
     5581 tcacagcgct agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg
     5641 tctgaaatcg accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa
     5701 aaatcatcag agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa
     5761 taatcgtaga catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa
     5821 ttaagcgcat ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg
     5881 cacctaccaa cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg
     5941 ttaccaaaat cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat
     6001 tcaaacgagg gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg
     6061 tcaccaagac cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt
     6121 ccgtcagcag ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc
     6181 atttagtgga gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa
     6241 agcagaacaa aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg
     6301 aataatcaac attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa
     6361 attaggcttg gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt
     6421 tacattactg ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa
     6481 gatgacaaag gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc
     6541 atacgctgat gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt
     6601 catgctggga tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt
     6661 caatccccac caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac
     6721 acctacattc cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc
     6781 tgccaaattg gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca
     6841 taaacataaa ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac
     6901 gctgttaaca aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa
     6961 tgtccactgg ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag
     7021 cagtaaaaat gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc
     7081 tttcatcccc tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat
     7141 aaccagcaaa acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg
     7201 cgtttatttg aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc
     7261 agtccgtgcg catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc
     7321 cattcaggaa ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca
     7381 tgagaatcgg cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa
     7441 aagtctggct gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg
     7501 taagtgtcct cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg
     7561 cgatcaacca aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg
     7621 gcggctcctg tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat
     7681 cgctgagcat ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt
     7741 acttcgtata acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct
     7801 tgctggtgct caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga
     7861 cagccgtcta gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc
     7921 cgcctgccaa agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac
     7981 tggcaccgcc gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag
     8041 ctctaccttg cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga
     8101 taaaatatat tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa
     8161 ggttgtatac ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga
     8221 gtcaatttgg tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc
     8281 acctttttca agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat
     8341 ttttttgtag taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc
     8401 cgcagccaat acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg
     8461 atagtcaccc tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc
     8521 ggtgataaag gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc
     8581 ccaatccgga atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc
     8641 agccaacgga ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc
     8701 tgattcaaac ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc
     8761 gtattctttt tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc
     8821 caagctggca atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc
     8881 tgccgcagcc gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac
     8941 tgttgctgca caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca
     9001 ttcttctccc ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc
     9061 ttgaaaaggt ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc
     9121 ccgttaacct tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag
     9181 tattatgatc agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac
     9241 tatttagcct atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca
     9301 tctaccggaa aagatgacat tccggattat ccagcctata taaacaaact ggactcttta
     9361 aaagaaacct attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa
     9421 gaagagcgga tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt
     9481 catcaaaatg gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa
     9541 actaccaagg aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat
     9601 attttgactc actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg
     9661 gagcattttg aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg
     9721 atggagttaa atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc
     9781 gttcctttgt atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt
     9841 gccgaagatt atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta
     9901 gaaaagttgc tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga
     9961 ttcctatata atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag
    10021 aggcacccat ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt
    10081 gccgatcctt gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca
    10141 gcttgcagcg ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat
    10201 atttgcatgc gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt
    10261 tacgaggaca gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg
    10321 gaatggctcg ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc
    10381 aggatcaagg cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg
    10441 aagaagtttg tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc
    10501 aagctcccct ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca
    10561 cacctttcgg ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct
    10621 ggtgatgaag ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt
    10681 tatcatttta atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag
    10741 ttttttattg tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact
    10801 tggagcttgg aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc
    10861 aagggttaga aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga
    10921 ttgagccagt gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact
    10981 gacttcagca aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc
    11041 cataagataa agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc
    11101 aaatgaggaa ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc
    11161 gctggcgtgt ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag
    11221 cagtggtacg gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca
    11281 atcgtcagca gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc
    11341 acttcgcagt taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta
    11401 tctaagccgg tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat
    11461 gtatccacat agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc
    11521 agtacggtat tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc
    11581 tgaaccagtg cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca
    11641 gcatccttct caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt
    11701 tcaatctgct tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc
    11761 tttaaataaa caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt
    11821 aatactgttc cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa
    11881 ggaaaaactt accatttaat cataaaatta gctcatataa atatactagg atgtaaaata
    11941 gactggtcaa aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt
    12001 ttaatattct aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac
    12061 tatatcacta gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta
    12121 aatggtcatt gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg
    12181 ctgagagtaa aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt
    12241 atttcagatt accttaaatg attccattta ctttttatct cattttttcc tactagttag
    12301 tatcaaatac tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag
    12361 ctaaaacctt gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt
    12421 tatggatgat gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag
    12481 tgatagctat gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga
    12541 ttgatgatct agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg
    12601 aatttattta tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc
    12661 cagtgattct gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac
    12721 aaaccattaa ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tact
//
LOCUS       DASJUF010000073        12747 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.073, whole genome shotgun sequence.
ACCESSION   DASJUF010000073 DASJUF010000000
VERSION     DASJUF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.073"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B62_001947"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B62_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2142096.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="U4B62_001948"
     CDS             complement(323..892)
                     /locus_tag="U4B62_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN2142097.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="U4B62_001949"
     CDS             1078..1980
                     /locus_tag="U4B62_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2142098.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B62_001950"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B62_001950"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN2142099.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B62_001951"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B62_001951"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN2142100.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U4B62_001952"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U4B62_001952"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN2142101.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="U4B62_001953"
     CDS             8554..8739
                     /locus_tag="U4B62_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN2142102.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="U4B62_001954"
     CDS             complement(8801..9997)
                     /locus_tag="U4B62_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2142103.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="U4B62_001955"
     CDS             complement(10167..11603)
                     /locus_tag="U4B62_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2142104.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="U4B62_001956"
     CDS             11896..12705
                     /locus_tag="U4B62_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2142105.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DASJUF010000074        12609 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJUF010000074 DASJUF010000000
VERSION     DASJUF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.074"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..306)
                     /locus_tag="U4B62_001957"
     CDS             complement(52..306)
                     /locus_tag="U4B62_001957"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2142106.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(710..1492)
                     /locus_tag="U4B62_001958"
     CDS             complement(710..1492)
                     /locus_tag="U4B62_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142107.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1557..2393)
                     /locus_tag="U4B62_001959"
     CDS             complement(1557..2393)
                     /locus_tag="U4B62_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2142108.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2411..3820)
                     /locus_tag="U4B62_001960"
     CDS             complement(2411..3820)
                     /locus_tag="U4B62_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2142109.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3834..5657)
                     /locus_tag="U4B62_001961"
     CDS             complement(3834..5657)
                     /locus_tag="U4B62_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2142110.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6500..6826)
                     /locus_tag="U4B62_001962"
     CDS             complement(6500..6826)
                     /locus_tag="U4B62_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142111.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6872..7234)
                     /locus_tag="U4B62_001963"
     CDS             complement(6872..7234)
                     /locus_tag="U4B62_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEN2142112.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7271..7485)
                     /locus_tag="U4B62_001964"
                     /pseudo
     CDS             complement(7271..7485)
                     /locus_tag="U4B62_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7613..7948
                     /locus_tag="U4B62_001965"
     CDS             7613..7948
                     /locus_tag="U4B62_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142113.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(8024..10021)
                     /locus_tag="U4B62_001966"
     CDS             complement(8024..10021)
                     /locus_tag="U4B62_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142114.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10119..10394)
                     /locus_tag="U4B62_001967"
     CDS             complement(10119..10394)
                     /locus_tag="U4B62_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142115.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10626..11360
                     /locus_tag="U4B62_001968"
     CDS             10626..11360
                     /locus_tag="U4B62_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142116.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11399..12541
                     /locus_tag="U4B62_001969"
     CDS             11399..12541
                     /locus_tag="U4B62_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2142117.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         4088 a         2506 c         1912 g         4103 t
ORIGIN      
        1 aagaaacagt gtaatcctta caccattttt caataaattg aacaagcgaa tttaggtttt
       61 ccgagagaat atggctgcgg ctttttttaa gatttcaagt tcctcattta aacgagcatt
      121 ttcttttctc agttcagcta cttcttttcc agtcagtcct gtggactgat caggtaaata
      181 taaattcttc catttgtaaa tcgttgcttc agataagcca tattctttcg ctaaccctcg
      241 aacagaacgt ccagtttgat tcagttcaac aatcatttgt ttaaaatttt cttcatatcg
      301 ggtcataaaa attcatcctc tcgtagataa gtttaattta ttcttgtctc tacgaaaagc
      361 tgtctaagat ttgatactaa caccaagtta tctttagaac catttcagat acatatgaaa
      421 caattacata tgattttttc ataaaaaaac agtactgtct agtagtataa aatatacaaa
      481 tttcttaaaa attttttcac taaaatcact acctgaaaca gtctaaaaca cttatatatc
      541 aacgtttaat gaaactatct ccgtttcatt tcagatacaa ataaatatag tcgggctcca
      601 aatccgaaaa aaaatacccc cagtagctta tcctaagcta ctggagatgt ataagtacgg
      661 tcatataaca acggggtagg gtaaataaat gaaaaagatg gaagaatcat tattcaataa
      721 cttttttaaa tacttcgttg taatcagtta cctgtccgtt cttaaatacc acagaataat
      781 cttttttccc attttcaagt aaccgaagtg gaatgtcttt accaacttgc tctgtcactg
      841 cattactgat acctgttaga ttagctaata aatctgataa agattgagca tctgtagaaa
      901 tctctgtatc agtatttatc gtgattcctt tatcttcatt tacaagcttc caagtaggtg
      961 gtatagctgt gtatgtttcg atgaatgaat caattctttc atctatcgtc ttcccagtat
     1021 ctacttctgt ctcattactc ttagatgaat cgattctttc atccatcgtc tgctccgtaa
     1081 ctgcttctgt ctcattattc tcaccggaaa ctctcttcac acccaaactt atatattgtc
     1141 catctttagt gtgcatagtt aaaattccca cgcttttact aataaattca ctataaccag
     1201 ttctttcaat agagacttca atgtatttca cactatcata ctcaaaagaa aaatagacat
     1261 gtaaaagcat tttgttgttt acatctacat aataatcctt tgactctact tgagaccttc
     1321 ctatacggaa tacagcttta ctgtctgata tctctatacc agcatcctta ttattaatct
     1381 gccaatctcc aagtagctct tctggcaagc tagtagtttc ttgtgaacac tttaccactt
     1441 cactggtaga ggtcgaaaca ttcacctccg cactgttaga agaattttcc aaggaatcag
     1501 gagccttagc acaaccaaca agtctttata tattgtgcag ccgatatttt cttcattcag
     1561 tacacccttg aaatatgcag tgttagataa agcagttcgt cctgatttgc tgaggcaccc
     1621 atctcttctt gaacgtactc cttgattttc aaggccgccc gatactcttt cggaaaacgc
     1681 tgcttcactg aagccagcaa aaaatcatcc ttctccggca actcttcctt cttaatcatt
     1741 cgggcaataa aaaactgtaa atgcgtcaca aatcttaagt aaagcatcga atctttatca
     1801 accttttttt cgggaaacag atatcctagc atagccaaga tatcctttag tgctcttacc
     1861 gagagcatgg tttcttccat atttgcttgt tcactttggg catttacaat gtgaaacgca
     1921 atgtttccag cctcctcttc tggcaactga acattcattt ttttgttgat caattggata
     1981 gcatagcagc cgatctcaaa ttcttttggg taatactttt gaatttcaat cagcaggcga
     2041 ttttgaaaaa caatgccttt ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag
     2101 gtaaaaaata gacttttatt cagcttaaag tcaaacttgg cgttggcata atcgataatt
     2161 cgcttgacgc aaataattag atcatccgga gcagcctcaa acagtttaat gtaatccttt
     2221 tttagagcat cagaaagtga atctaattta aatacttttt ggattttgtc agccggaacc
     2281 aaatcgcctt tctttagttg aaatccaatc cctttcccca tcaaaattac ttctttgcct
     2341 tgcttatcta agcaaagaat taaactgttg tttaacgttt taattactct cattagattc
     2401 accgttctct ctaagctaag ccatttcttg cgattacttt ttgataccaa aagaagcttt
     2461 ctttttttat tcgttttaat tctttaagat tctcatcggt tcggtcaaca tatataaaac
     2521 cataacgctt attgaagcct tgatgggtac taacaacatc aatcgctgac caaggtaaat
     2581 aaccaaaaac atctacacca tctgttaaag ctgcttgaat agccagaata tgtttggcta
     2641 gataatcaat tcgatcctgg tcattaattc gtctatcttc ctcaactatg tcgggtgccc
     2701 catagccatt ttcagtaatt aaaataggta aatcatagcg atcgtacaat ttcctcaatg
     2761 ttaatcgtag tccagtaggg tcaactaccc agccatatgg cgttttatct acataaggat
     2821 tttcagctgc ccggtagaca ccttcttctc ccaacatgat ctgttgatcc cccgctctag
     2881 cggaaacatc tgatccgtca tttttgctgg cagcgatggt ggcagttgaa taataattaa
     2941 tcgcaataaa atcgggttta ccggaggata aaattgcctg atcctctgcc tcaattttag
     3001 gatataatcc acgatcattc aaatagtttt caaacaaata attgtatttt ccccgaactg
     3061 ctacatctaa gaaagaccag cctctcaatg tttcccaatt atgggctgca attgcatctt
     3121 ctggactgga ggtagcttga tacatagccg ttaaattgat tgctggaccg atttttcctt
     3181 taagattcaa ctgatgatac aagtgaatta ctctagcttg agctaataaa acatggtgat
     3241 taatttgaaa gagttctttt tttgaaggca gctctccact tggtggcaaa ccaatcgctc
     3301 caggatgaag gatcatcgtg ttttgctcat taattgtcag ccaatagttc accttgtcac
     3361 catactcagt aagcaatacc tgtacatagt ccacaaaata atcgatagtt gctcgattta
     3421 accaaccgcc ctgctcctct agaaaataag gtaaatcgaa atggtacatg gtgacaatcg
     3481 gttcgattcc ctgattcagg cattcatcaa ttacttgatg ataaaaagaa attccctttt
     3541 gattggtttt attattttca atatccataa ttcgcgtcca agcgatggaa aagcgaaaag
     3601 cttttaagcc taattcttta aacagctgta tatcttcttt atagtgatgg taaaaatccg
     3661 aggcaacttc aaaattagct accccttctg tatgctgata cttgtcaata atagacggtt
     3721 gtttcccatc ctcctgatag gcaccctctg cctggtaggc actaatggag gcaccccata
     3781 aaaacttctc tggaaatgct gaaagctgtt tgtattgcac aaaaatcctc tccttaacta
     3841 atttgcatca atggttcgtg ttcctttaag cttccgcttg cttcactaat tcctgcgtaa
     3901 ttttcactgt tggtcactac cacaatcact gtatcatcgt agccagcgtt tctaatggca
     3961 gcgaaatcgg cgttgattaa taattgtcct gctgacacct gatctcctgc ttttacaaat
     4021 acttcaaagg gttccccttt taaattcacg gtgtcgatcc ccacatggat caataattca
     4081 ataccttctt tagaacgtag accaatcgca tgtttagatt ctaaaatagt aatgacttct
     4141 ccctcaacag gtgaataaat gctgttttct tctggatgaa tggctacgcc ctttcccaat
     4201 agtcccttag aaaagacagc atcgttcact tcagaaatag gacacacggt tccttttacc
     4261 ggattttcaa caatgactgc ttcggctatt gccgtttgct ctgattcatc agaagtatcg
     4321 tcaaatccaa ttagataagc aatgataaag gttgcaacaa aggcgattac acacgtaata
     4381 attgcgtgaa caatattcat tggattttct ccaataaagg ctggtaaggc tgctaagcca
     4441 ggggaaacaa aggcatatct gactaaaccg ctcaagccag catacaaacc agcaattgca
     4501 ccaccaatca tggaagcaac taatggtttt ttcagcttca aggttactcc atagagtgaa
     4561 ggctcagtaa ttcccattaa agcagtgatc ccagtggaca cagctagctg cttcaactct
     4621 ttgtttttag ttttcgcggc tacagcaaag gtagcacctg attgagcaat attagaagcc
     4681 agcattcctg gcccattgat catttcagaa cccgtactgc tcaattgcat agtagcgatt
     4741 ggcgtcattg cccaagctgt acccgtaacc accaaaaatg gctgcaaacc acccatcaaa
     4801 aatggaatta accaagctgc tttgccgtta atccattcag cccccattgc tataagatcg
     4861 ttcagcaacg ttccaaacgg tccaatgaca accaacgcta aaggccccat aattaaaaat
     4921 accagcatcg gcttagtaaa gaatttaata aacgaaggcg aaattcgatc cgcaaagggc
     4981 tctacatagg acatgatcca aacagtaaag ataatcggta aaactgatcc cgcataatct
     5041 gcagctaata ccggaattcc tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa
     5101 atatttggat gaagcaaaat gccagcaaca gtaatcgcca aaattgggtt gacatcaaac
     5161 tttatggctg ccccataggc taatagtact ggcataaaat aaaaggctgc atcagcaata
     5221 gtattcaaaa tgatgtaagt ttgactactg tctgatagta caccagtcaa agtcaagacg
     5281 gccaaaatgg cttttaccat cccggcacct gttatagctg gaatcatcgg tgtaaaggtg
     5341 gtagaaatga cactaatcaa tcttgaaata atactttctt gattgtctga agaggggccc
     5401 tttttaattg aagttcccac ttccttttga atttccttga aggctgtttg gacttcattg
     5461 ccaatcacca cttggtattg tccaccgcta tccaccacac taattacacc aagtgttttt
     5521 tctaaggctt tcgtatcggc tttcctttta tctttgagtt caaaacgcag tctggtggca
     5581 caatgggtta agctgaccac attttctttt cccccaacct gttgcaatac atttcttcct
     5641 acttctctgt aatccattat tctctctcct taaagcagta aaaaagacca agtcaaacca
     5701 cgccccaatc aacattgaag tgttagtttg tcttggtctt gcctgcataa tcagtaacat
     5761 gccaataacg ttattttgct gtaatccaat aatagcacat tatgatagcg caatcaacag
     5821 ggaatttaat tttttatctt ttcgtttgac cgtatagggt aatataacag ttagatcaca
     5881 tcaactaggg tatatctgcg tatacttact gctaaaataa gtatatctaa tattaactat
     5941 accagtaaaa ccattcctac ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa
     6001 aatgcatata acggatcaca caacttcaac aattctgctc ttgggaaaaa ttgatgaatt
     6061 tggtgagtaa aggatggaat aaacagcatt ccaacaatat aggcaacaat agtgatactt
     6121 gtttttttgg taaaaatcag taagcaatag ccaatttcaa tcattccgag atataaaggt
     6181 atcgatagaa tgatttgggc tagtattctt aaaaattaac tagcaaatgt ttaaactgat
     6241 ttatttctaa tctacatgta gtaagtattt caattccacc aattatcaat aaaaatacca
     6301 aacatatcca taataatttt ttctttttca tttcttttat attgttataa cagtgaataa
     6361 cataaaaatt cccgacaatc attgtaatat ttttttgtca cctgtattag tatttaattt
     6421 ataggaaact ataaaaactc cctagtgaat ttacaccttt gggagttcct gtttggacgg
     6481 tatgcataat acatttaaat caatttaaca ttaacacctc agcatatggt atctccacat
     6541 atatacggtc tttctttgga tagtactcaa tatttatgtt gatagcactt ccattgtcta
     6601 atatagcttt agcccggtat gtgttatctc cattttcatg agtaatcgtc acatcatcac
     6661 atgatcgatt taagttgtac tgttcttgta aaatctgtgt taccaatggt ttcgatgact
     6721 tctctaatcc agatgtacct gtcatttttg aaaaaatgat acctataata gccaagacaa
     6781 cgataactat aatgataaac ttcttgttca tatttacttt attcaacttt ttatctccta
     6841 tctccaaaat aatttgctct tatccctctt atcagtacat tgacatccct aaaagtacac
     6901 caaagattgc tccagctacc cctgctatca tcaaaatcgt acccatttgt tcatctttat
     6961 ctcgatataa gtagcccata ataacagcaa cagcaccaaa tacaacagga ataaatgcta
     7021 acgctactac catagaaatc caaccaataa tcaaatacag cttccctgtc tcttgtccat
     7081 gtacgatttg ttccttcatt tcattaattg tctgctcagt acttttcttt actgcgtctg
     7141 ctccagttgt ctcatctgtt tcttgtagtg ctccgcaaaa ctggcaatac ttactttcat
     7201 ctgttatttc tttgccacat tttctacaat acatgtcttc tcctcctaaa aacattcaat
     7261 tgtttattag ttaactgttt actttattaa atacatcaga aaacagctca ttcattggta
     7321 cacttaatat ctcagaaagc atcttcattt gatagggttt aggaatcgtt ctccctgttt
     7381 gccacgaact tacacattgc tgtgtaacat tcattttttt gctaactccg tttgagaaag
     7441 tcctttttct tttctgtatc ggctgagatt acttactttt ttcatgattc tcccctcctg
     7501 ttttattata tacaacaata tgatgtattt caactcataa aatataaaaa aacttcaaat
     7561 accattacaa atacaacaac ttgttgtact atactgagat gggggtgtat taatgaaaca
     7621 gaaacaatat gcaaaggtca ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga
     7681 attagctgaa aaaatcttct ctacacaaca ggcagtagca agatgggaaa attctgtaac
     7741 agagcctaac ctcgatagct taaccgctct ttctagagcc ctaggaaccc ctgtaagcca
     7801 ttttctagac aatgttgtcg tagattatga ggaagagttt ttggctctct atcgttctct
     7861 tagcactgaa gatgcagttc gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa
     7921 tgaacgcaat cagctattga aagattgaaa aataaataac ccaccgaact ttaacggtgg
     7981 gtgtattgag atgaaataag attaagagct atggataacc tgtctagttt aatacaattg
     8041 ggtcttttct cttccttact ctccgtccat catctcttgt tacacttgta aattgataaa
     8101 gaggaactaa ttcaactcct gataatatca attcttttat tacttcatct atccctgctc
     8161 cttttgctag gtagactaaa aaactactac ttgctttcac attgtatagt ctcttaatat
     8221 ccttagaagc tcgttcaaga gtgaatcctt gtttaacaaa gtagcctctg ttttctttta
     8281 aagcattttc cacgctgtta ttcccaacat tacgtagacg ttcacaaaca gtgttagtaa
     8341 gttctttctc tgtcattaga tatgttcctt gccacgataa gccctgtaaa acttcatgtg
     8401 cttcatctaa aatcaattgt ttttccttgc taagttctga tcgggaattt tgaggaaata
     8461 ctcttttagc ctctttttcg ttaaaacata tcttaaccca ttcataagaa aaatattgat
     8521 atgtaaaatg aaatttttcc agaagtggaa ggatatcttc attcaatttt tgaatcacag
     8581 aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgatta aagtttccta
     8641 acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga acatcttcat
     8701 catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta ttacatttat
     8761 ctatgtcata agttttacca tagtaaaaac tcattttttt agctaattgt tcccccgaca
     8821 aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa agcaagacat
     8881 catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt ttgtctttca
     8941 ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga aaatgtgtag
     9001 gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg tctttcttta
     9061 aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg gaatgacaat
     9121 aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta tgatcgttgt
     9181 gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca atggttaaca
     9241 atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag tctttaaacc
     9301 actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg gtttcattga
     9361 ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc atggtaaaat
     9421 caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc actgcatctg
     9481 acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca cacccgggga
     9541 tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta attgcttctt
     9601 ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata cttttttgta
     9661 tcgcaaacgc agaattatga actaaattga tagataaaac cttttctatg cttaagtatc
     9721 tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca gtgttatcga
     9781 catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct ttattctttg
     9841 tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca tctcttgtaa
     9901 cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt tttgtaactg
     9961 gcttctctaa taatttttct ttatctacat aaattggaat attaatcatt gatgtttcca
    10021 tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata ataaaaacaa
    10081 actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata ccgttccatg
    10141 aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat aacaggtaac
    10201 ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata ttcacttgcc
    10261 tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag cataggcttt
    10321 agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat gctatcaatg
    10381 atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt tctaagaatt
    10441 atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta atcaaacaaa
    10501 aaatgattac cagttcatcg acatacacat aatacctcga taaaaaagag gtgtcaatat
    10561 ctttgtaaaa tttatttaca acaccccttt ttcgaggtat actaatttta ggaggtgtgg
    10621 tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata ctcggaacaa
    10681 aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt cttatgatta
    10741 acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga acctatttac
    10801 caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata gaggagttag
    10861 tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat agcattgtac
    10921 tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa cagttcccac
    10981 cagtacactc taatttagat acatggctgg atagatattc caaattagaa ccagagatgc
    11041 aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt agtcttttca
    11101 atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt gacaataaca
    11161 ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat gaatggctac
    11221 ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt actgataata
    11281 taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag aaaaaaatga
    11341 agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag atgatttcat
    11401 ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca
    11461 cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag gttttagtag
    11521 caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat acggtgttaa
    11581 aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa ctcatcagcg
    11641 taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata atcaaatact
    11701 tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc aaaaagtggt
    11761 agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga ctgcacaaat
    11821 tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa aaacacttct
    11881 tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg agcttaataa
    11941 tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct tcagattatt
    12001 ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag acatagacac
    12061 gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg gtaaagttat
    12121 aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata ctgagactct
    12181 ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac ttggatacaa
    12241 tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat acattccaaa
    12301 cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc gtattacacc
    12361 tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat ctgtcaaggt
    12421 agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct atgcacatat
    12481 tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg ctcatcagta
    12541 aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa agctagaacc
    12601 cttatatat
//
LOCUS       DASJUF010000075        12600 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.075, whole genome shotgun sequence.
ACCESSION   DASJUF010000075 DASJUF010000000
VERSION     DASJUF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.075"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..893)
                     /locus_tag="U4B62_001970"
     CDS             complement(324..893)
                     /locus_tag="U4B62_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2142118.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="U4B62_001971"
     CDS             complement(967..2256)
                     /locus_tag="U4B62_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142119.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="U4B62_001972"
     CDS             2610..3458
                     /locus_tag="U4B62_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN2142120.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="U4B62_001973"
     CDS             3472..4545
                     /locus_tag="U4B62_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN2142121.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="U4B62_001974"
     CDS             4535..6706
                     /locus_tag="U4B62_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEN2142122.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="U4B62_001975"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="U4B62_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN2142123.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="U4B62_001976"
     CDS             complement(9117..11303)
                     /locus_tag="U4B62_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN2142124.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="U4B62_001977"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="U4B62_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN2142125.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="U4B62_001978"
     CDS             complement(11525..11965)
                     /locus_tag="U4B62_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN2142126.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="U4B62_001979"
     CDS             complement(12039..12512)
                     /locus_tag="U4B62_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HEN2142127.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT         3537 a         2652 c         2235 g         4176 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
//
LOCUS       DASJUF010000076        12343 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJUF010000076 DASJUF010000000
VERSION     DASJUF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.076"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..154
                     /locus_tag="U4B62_001980"
     CDS             <1..154
                     /locus_tag="U4B62_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN2142128.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /gene="istA"
                     /locus_tag="U4B62_001981"
                     /pseudo
     CDS             complement(235..1218)
                     /gene="istA"
                     /locus_tag="U4B62_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="U4B62_001982"
     CDS             complement(1613..2455)
                     /locus_tag="U4B62_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2142129.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="U4B62_001983"
     CDS             2780..3196
                     /locus_tag="U4B62_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2142130.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="U4B62_001984"
     CDS             3193..3663
                     /locus_tag="U4B62_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2142131.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="U4B62_001985"
     CDS             3677..4450
                     /locus_tag="U4B62_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2142132.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="U4B62_001986"
     CDS             4464..5276
                     /locus_tag="U4B62_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2142133.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="U4B62_001987"
     CDS             5330..6295
                     /locus_tag="U4B62_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2142134.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="U4B62_001988"
     CDS             6431..9154
                     /locus_tag="U4B62_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2142135.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="U4B62_001989"
     CDS             9167..9799
                     /locus_tag="U4B62_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2142136.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10004..10231
                     /locus_tag="U4B62_001990"
     CDS             10004..10231
                     /locus_tag="U4B62_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEN2142137.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10300..10566
                     /locus_tag="U4B62_001991"
     CDS             10300..10566
                     /locus_tag="U4B62_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2142138.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="U4B62_001992"
     CDS             10556..10825
                     /locus_tag="U4B62_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2142139.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="U4B62_001993"
     CDS             complement(11004..12305)
                     /locus_tag="U4B62_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2142140.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4032 a         1999 c         2422 g         3890 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgattca att
//
LOCUS       DASJUF010000077        12334 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.077, whole genome shotgun sequence.
ACCESSION   DASJUF010000077 DASJUF010000000
VERSION     DASJUF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.077"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1273)
                     /locus_tag="U4B62_001994"
     CDS             complement(<1..1273)
                     /locus_tag="U4B62_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN2142141.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIY"
     gene            complement(1370..1870)
                     /locus_tag="U4B62_001995"
     CDS             complement(1370..1870)
                     /locus_tag="U4B62_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN2142142.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            complement(1883..2104)
                     /locus_tag="U4B62_001996"
     CDS             complement(1883..2104)
                     /locus_tag="U4B62_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142143.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            complement(2109..2246)
                     /locus_tag="U4B62_001997"
     CDS             complement(2109..2246)
                     /locus_tag="U4B62_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN2142144.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(2243..3427)
                     /locus_tag="U4B62_001998"
     CDS             complement(2243..3427)
                     /locus_tag="U4B62_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2142145.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(3683..3937)
                     /locus_tag="U4B62_001999"
     CDS             complement(3683..3937)
                     /locus_tag="U4B62_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142146.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(3962..5104)
                     /locus_tag="U4B62_002000"
     CDS             complement(3962..5104)
                     /locus_tag="U4B62_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEN2142147.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(5164..6516)
                     /locus_tag="U4B62_002001"
     CDS             complement(5164..6516)
                     /locus_tag="U4B62_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN2142148.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(6587..6931)
                     /locus_tag="U4B62_002002"
     CDS             complement(6587..6931)
                     /locus_tag="U4B62_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN2142149.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(6941..7315)
                     /locus_tag="U4B62_002003"
     CDS             complement(6941..7315)
                     /locus_tag="U4B62_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2142150.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(7328..7642)
                     /locus_tag="U4B62_002004"
     CDS             complement(7328..7642)
                     /locus_tag="U4B62_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2142151.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(7756..10983)
                     /gene="fss3"
                     /locus_tag="U4B62_002005"
     CDS             complement(7756..10983)
                     /gene="fss3"
                     /locus_tag="U4B62_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN2142152.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(11062..11724)
                     /locus_tag="U4B62_002006"
     CDS             complement(11062..11724)
                     /locus_tag="U4B62_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142153.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         3440 a         2777 c         2025 g         4092 t
ORIGIN      
        1 cataaatttg tttttgagag tatatactca ccttatcgga aatcattctc aaccgttctg
       61 tttctttttc aaccagacca ttctcttgtg taaattcata aatagaatgt ctatcctgtg
      121 ttcttattat ttgatatttt gtccccttat agaaaatatt cagtttcaca attgtatcct
      181 tcaaaaatat tccagagcca ttatgttcaa tttgtgcaaa gttctgataa tcactttgta
      241 tttcagaaaa ttgatcaagt tctgcttcct ttccaaggac atagaccaag aatcttgtaa
      301 tacaagattt gcctgttcct cttcctccaa taattgaagt taaattatcg ctgaattcga
      361 ctacaacttc ttttgtgcta agagtggtcc cagaaaagct taatttttcc aataaaactt
      421 cattttcatt aatggagaaa ttagaaaaat cagaatgaac tcgagtttcg cctagcatta
      481 aagcatcgcg aagtgatccc aagtcaggtt cgtccttcat ttttatatag gaatatcttg
      541 tgcctattcc ccaaagtcca tgttttgaat ttcctttcga gtgtgggtta tcagaaaagc
      601 taagaaatgt catattattt ttttctgcct caacaactga tttataccaa gattccaaag
      661 tatcttttcc aactctacca tcatatctgg cattcacact atcatagata gtgtcacgct
      721 tagatgaggg aatcatttgt gagcgatttt caaaaaattc tttttgaacc gtctgtactg
      781 catatatatt ttctgattca aatatctttc ctagatttcc aaattttgct gaagaaattc
      841 cattaaactc atctatatgt gcaggaataa cgagcaagcc ctcgtgctca gcaaaatcaa
      901 taacttctaa aactgatttc attgagcaag ctgcagcgct ggcccaatcc tctcttttta
      961 tacccatctt ggttaagaaa tcagagatat catttgtaca tgcactttta tcaaatagga
     1021 ctagtaaatg agttccattt tctccacacg ttaattccac tccaggaaaa aaatctaagc
     1081 cattttcgat agccactttc ttatattcat caatattttc ccctgtattg tgatcagtta
     1141 atgcaacaca atcgagtcct ttgtttaaac attcttctac ccattcctta gcagtaacct
     1201 ttttatcctg aaagcactga ctcgcagttg aatgaatatg aagatcacat ttgtaccact
     1261 tattgccaac cattagtaac acccctatca acaaatattt tctatttcag tatatcaaat
     1321 agaaaagtaa aagtacgata gaaatttctg gtcaattcta acaaatgatt tacattagtt
     1381 caaaaacccc tcgactcgta accaaataat tcccgccaat ctccaagtct cgaccacacg
     1441 catcaaaatc aatatacgtc ttcaagtgtt ctggtacttc gcctaaatca ccagcttcta
     1501 caatcaagta atacgctaca tcactcatgt cattacaatc gggataatag atgatatcat
     1561 ccacatgctc taccatttcc tcgaaagaat tgaagaaggc atgttgaatc tctgaagcga
     1621 cctcaccaat tggcgttcct ttaagctctt ccgctaaacc acacaaccga ttaatttcct
     1681 caattggtgt atactcatca atctcaaaag gcagttcata atcatgaatt gcgtactctt
     1741 catattcgtc atttaatcca atccgttctt tcacttcatc aaaatcgacg ggtggggtaa
     1801 accatgcccc caccaactca ccttcattgt attttccaag gttcgcaata taaacacgta
     1861 tctgttccat tgtgattccc cctcaggcac cgacaattcg gttaaacaaa ttgagtaaga
     1921 catccttcac accagaagca ttaaagacca gacctaccgc aatcaaagca atcaccaaga
     1981 agccaatcag tttggaaaac tcgcgtttaa atcctaaaaa caaaccaatg accacaatcg
     2041 ctagtaagac taaagattga gcattggtta aaaaccattg atacaaattt tgaccgaaat
     2101 tcatttcatc actttccttt cttttccatt gctcggtccg ccgctgcact tgcatgcatc
     2161 aagcacatca cagtaacacc aagcatactg ccaccaaaca ccaataaaat atctttcaca
     2221 atttccatca cgattcctct tttcatataa tcaaattttc aattgccata ctttgttgct
     2281 ccaaaatctt tttgtgccgg tctgatagtc gagtattatt taccatatcc tgaatgtacg
     2341 ttgtctgatt caaccgatcc aactccttgg ccatcttcca cgtaggggcg acttgtcgtc
     2401 caagccagtt caacgttcgt tcaatggtat aaggcttcgg ttcagaagtt aattgaattt
     2461 ccttctgatt ccgtccaatg aagcgccccc attgttcatt caagggccag ttagttcgcc
     2521 ttttgccttc caccttatcc gcaaatcgca agtaccggtt aataatggaa aatgtggtac
     2581 tttcaatact gcgacctttt aacaaatctt gaattgcatg ataagctcga tcatttttca
     2641 gtcggatttc aaaacgattt ttcgtttcgg tctggtctaa aggaatccct aacttgatat
     2701 attgctcata gtctttttcg tacaaacaga aatagacctc actcttaaga ctgccaatat
     2761 acaaagtatt gcccatacca tctttatcat caggtttcaa cagttccccg gaacgatagc
     2821 ttttgaaggt acgaaacaag gagatacatt cttccttctg acattttttc gttaaatcag
     2881 gaatatctaa caggccaact cgatcattga ttgccaggtc taagcgtttc atcacgccac
     2941 cggcttttag acaatcttcg aaaaagtcat accagctacg cttttgggcg agtaaaaaag
     3001 tttcaaactg ccgacaacca cgacccttta attctaaaag gacgccttta tcttcttcat
     3061 gtgacaacat caccacaata tctcccataa catattgttc ttgataggag taaaaggcgt
     3121 aatcttcgtg gagcatataa tcaaatttca aatggagaat ctcttgaatg attttcctcg
     3181 catccgttgt gggaaagcga atccttacat agtcaaacag taacgttaaa ggtaactcgg
     3241 gattgaaact ttccagttgg tcaaaaattt tcctcatggt ttttgtcgat gcggttacgc
     3301 caccattttc tatctgattt aagtacgggc gactaagtcc acaagtcgta gctaatttat
     3361 tttgtgaaac gccatattca ttacgccggt cttttaatag ccgataatct aaattccttt
     3421 gtgccaagtt ctcacctcca aatttaaggt gttaactttt tcaaatcagt taacatctac
     3481 acaaattgct tattatcctt gataggacag ccattcctta tcgttgcttg cttatttttt
     3541 gtgtttttgt acccccctgt tagatacggg gggtgagaca tcatggccgg ctaacgccgt
     3601 ccataaacag gcacagcacc acggaggatt tctgcttcgc aaatcccccg cggtcagtgc
     3661 ctatcagtgg tttatttggc cgttactccg cggtaatacc ctcaatttcc aactctgctt
     3721 ttatctcaaa caagtattgg cggatatcag gttctttagc aaagtccgca attcgttcca
     3781 attcactgat gacttgattc agctccattc gagcatccgt ttcctcatca attacatacg
     3841 gatcatcagg gactttcacc ccgtttttgc gaatatcaaa attgagccgg tacagttctt
     3901 cttccaattc cggatctgac tgaatcttgc caatcaccca gtagattccc caatcaactc
     3961 atcataaatc atctgttttt acctccttta atcttctagc aatatcaaaa atgaccggaa
     4021 ctgttacaga atttccagcc tgcttataca actgactatc tgaattgact tgagaagctc
     4081 gatcaaaagc ccagtccgga aagccctgaa gccgccagca ctccctaggt gttaactttc
     4141 gaatttgata ctgatccgtg acgactgctt gttggtctcc tgttaataag gtatgcacac
     4201 tttgttttcc cagacggcct cgacgacttg tggaattagg aaaagccagg ttcacactgt
     4261 tccctggctt cacctcttga tattctgttt cgtttttccg aagcatgatc ttcgttccgc
     4321 cttgtttcgt tgacaaagta ggcgctgttc cagttatctc ataaacccga ttgccttgtt
     4381 caaaatttcc cagtaacata cccgctacag aaattccatc atctaccggc aatgcgattt
     4441 tgattccttc atcaccggtc aaggtaggcg ctacgtcatc tgacccatag acttgtcccc
     4501 ccattcctcg attggaaggg ttgatattta ataagtttct gatgttttga tcagatgttg
     4561 gagacgatcc aatgatagga aatacttgtt cggtacatgc gtctcgagaa tgtccgataa
     4621 ggaagatccg ctcacggttt tggggaacgt aggcctttga gttaagcact tgccattcca
     4681 catcataccc caatccatcc agcgttcgga ggatcgtctc gaacgtagcc cctccttcgt
     4741 gattgagtaa tccccggacg ttctcaagga ataaaaactt aggttggaga atagcggcga
     4801 accgagcgat ttcaaagaat aaagtacctc gagtatcgga aaatccttgc cgctttcccg
     4861 caattgaaaa agcttggcac ggaaatccac cgcaaaggat atccactggt ccgagagatt
     4921 gaataaactc gtctgataca cttgtgatgt catgcatttc cacctcctta cttgtatcat
     4981 ggattgcctt ataactctgg cgagcaaaat catcaatttc acaaaagccg atacactggt
     5041 gacccgcttg ttccatacct aagcaaaagc caccaatccc agcgaataaa tctaaaaatg
     5101 tcataaatca cctcgtaaaa aaagcccagg attcttgaca ctcctaggct acatactatt
     5161 attttatcct tccgtattaa cgtttaatcc accaatgtcg ctaatttccc ttaagaaatc
     5221 atatcctttt ggtacaagtg gcgtataaaa ctcactaatc acactccctc ccgtatctac
     5281 atagcctcga cctttgatac gcttcataaa gaatttttta tcaacctcgc caaacatcat
     5341 ggaatatccc agttcactca tacgacccaa agccacccag aaattaaatt gatcgcgaat
     5401 cccgtctcct aaatatttcg catccggtct ttgacaagct agaatcagaa agaaaccaga
     5461 ttgacgacct aacatcacga tttgtttgag cttattcaaa atcaccgcac tttccttcgt
     5521 cgttaacatt tccatataaa ccacgtattc atcaaagatt aaaaagtttg gtggaagtcc
     5581 aagaaacgca taattctctc ctgttttgta gttgggcatt tctttcattg ccttgctacg
     5641 agtcatcatg cgctcataga aatcttccac acaagcagaa atttcctcct tttgggaata
     5701 gacatgaggc attaccgtac ctaaatcggc taagtccgca tttttgggat caagaacaaa
     5761 aagctcggca tcagatttta ataaagcctc gatgatagtg agtaaaaagt aagtcttccc
     5821 accacctgtg ccgccagcga ttaacatatg aggtaaagaa tcataagccc atacttgatt
     5881 tttcatcaat cgcaaagccc cactttctgc cactacttcg tctatcccaa ttcgattggc
     5941 aattatatca tacagtaaag tgtactccac ataggagtct ttgagttcct tttctactag
     6001 ttcacaatat agaccacttt ctaacttctt ttccagcttt aaaagctgat cttgatattt
     6061 tcccagtgaa atttgaacgc gaatagaaag taaaccatcc ttcatacggt aatagatttt
     6121 ggggaagtaa ctgattgtct ctttggttcg actactattg aggtctttga aaaatccttc
     6181 actctgagtt tgttttactt cgtaccaatg attttctaga atcatccggg ctagcttttg
     6241 tcgatgccac aaactgcgcc aatgatcctg aaaaaagtgt atataaagca acatcgtgct
     6301 tcctgaaaga acgagacttc ctagcaaata gattaaatct tttatagaac gaacgatttc
     6361 tatagtagaa agtgatagct tagaaatatc aaattgtcta agctcttgaa aataaaagac
     6421 gctcatccaa ccgatgaaca gagcgagtag ccagccaaga acaaagtgat aaaccaagtg
     6481 ctgatctcct gcccgtatac gatgcccttt gtacattttt atggtttcca cctccaatcg
     6541 aaaaaagctt cataggattt cattgatcca atttcaagca gttatgctag tcatttactg
     6601 ctatagggag ttgtttcgtt tccactagtt gctttaaaaa aacttcaatg gcatccggca
     6661 tttccacacc aatttcttcc aatacggttt cggcttctcg acatagactt tcatttactt
     6721 cgataaccat aaagaaggac tcttcttccc ctaaccaatt cagtacatca aaatcttctt
     6781 cggcatcgtt acctacttcc agtaaccctt gatgcataat cagaaacatt ttttcatcat
     6841 aggttaatgc tttagtgagt tgttcctccg ttagctcacc ttttccatat tgatcgagac
     6901 aatctaaaat atgttcaaca ttaatttcca tacgcttcac ctatttcttt ccttcatttt
     6961 ggtttgtaga atttccctga tctggttgtc gtttcaagac taaatcatcg gccttaatga
     7021 accaattgac agtagttcct tctcggaaaa cataattggc gaccgtatcc atgattggat
     7081 tgaccaactc tacctctgcg tcataggcaa actcgcgtaa gggtacattt gctgggatac
     7141 taacttggat cattcgacct tgcgcagagg atttcaaatt ataagtccgt tcttttacct
     7201 cgttactaac cgttccatcc tcattttgct tccgtacttc tcggcgtaat gcggaaaatc
     7261 gtaatttccc aaacgttagt cctgaatcta caacaatacc ttctgttaat ctcatcttct
     7321 tttcctccta tgctttcact aaatcatcga catacaacac gtagtcggta aacccacgat
     7381 tctcaatctc atagcctttg gcaaccaaat ggggattcac ggcttttagt ggttgatctt
     7441 gatcaaaatc cttttcgccg gcttccgcag ctaccaccac ttcaatatca tcggctcgtt
     7501 gcttactgga atacaaatga taactacgac caattactgt agtattccgc ccatatcctt
     7561 cagttaaaac ttcgccttcg ccggcatacc ttatctttcc aaaggtttcg gccatatctg
     7621 gcacaacaaa tttcaattcc atcttctttt cctcttttca tttttagttt tggatacaaa
     7681 aatagcgcga ccactttttt taggaggcgg tcgcgcacgt tggtttttcc tatttagttg
     7741 tttcatcaca tcaccttagt ttcggcgatt ccagaaataa tacccagcag tcgcaaagat
     7801 caaggtaaac ccaataaaca gcagaacatt agaatttttc tcccctgttt gtgggaatgt
     7861 ctttactgta ggactagatt cttgagagtc acttggaaca gttggtattt ccggttgttc
     7921 tggtgcactt ggtgttgtcg gaactttttt aggtgttaaa gtttgagttt tttcttttag
     7981 atcctcattg tgtttcccat tgatgtttcc gtctttgcca tagttaattt ctttgaaggt
     8041 aaaacttgta ccttctggat atttacttgt gtccactttt tccgcaagta cggttttagt
     8101 gaattctttg tcattcactt cataatcaat tttgccagat ttccaaattt ctttggtcgt
     8161 attatccgga agtaatgcat acagaattgt ttcaaaagct tcttttgagc catccagtac
     8221 atcatgggta atcgacacat catcgaacat atccaccaca tccccatgag taaaggtttg
     8281 cgaaccatct tctaggtggg cttttgtttg gatggaaaca tggcgttcca ccgtacaatt
     8341 gacagtttgg gcttgattgt tcaagctagc atctttcgct actggatcat cacctgcttc
     8401 atagggtttc ttgttttcat acacatagtt gaataagacg atggttttat ccaaaacttg
     8461 ttctgcggtt aatttatgca aaattttcca agttcctgtt ttctctttat tcgccaacgt
     8521 tgcggttgtt tcggcaatta ccactggttt ggctttttca tctttttctt gggcagcttt
     8581 tgtcgcttcc acatcaatgg cttgcgctac cacataccag tcttctttga tttcatgcaa
     8641 gttatagctc aatttatcaa ctagctcggt agaatcaagg tctgtcaatt ctttgcctcc
     8701 gtctttccaa gtcccaagag aagttaagct atcttcttct tcaggtaaat catagagcat
     8761 caaacgattc aagctaacag aaaatgcttt attcgtcagt ttctcgtaag gaacggtcac
     8821 cgttttaatt ggttgttttt gtcctttttc ggtaatcgta aaaatgtatt cactcttagt
     8881 gtaatccgct ttattttctt taaaagtaga acggatttct aatggcgtaa ttttttgaaa
     8941 tccttctgga gcctctactt cttcaagtaa ataatcccca taaggaagat tttcaaactt
     9001 gccatagcca tcaaaaccta gttgctcgtt ataagctgta attgcttcat cttcggcatc
     9061 tgtaatctca ctggttcctt ccaatggcga tactttaaag gtcagatcat tgaagccagt
     9121 ttcggcagtc ccagcagctg atccagcaaa tttaaagaaa tcaaagccaa agcgaatgac
     9181 ttgttctttt gccgtaacat ctcgggtaat cacggcattt ttttcgttat catccacctt
     9241 tttgatggaa acagggtact ttgtttcatc caaggtatat ccttcaggtg ctttggtttc
     9301 ttgccagtaa tactcattaa tcgctaagtg tttaacagca acttgattct tttcatctaa
     9361 agtcaaagtc accgtttcat cagaagcttt cgttcccttc actaactctg gtttaaaagc
     9421 ctcactccat ttgaccgctt gaccatcttt tgcagtaaag agagtatatt cagctccttt
     9481 aaactcagct ttaccttgag tttcattacc ggtatctttg tcttcttttg tcaaagtggt
     9541 ttcaccagtg atttcttggt tttgcccttt tacgttactg gtaacaagag ccacggtttg
     9601 attgacatag ttcaattcga cttttactgg tttaaaggta ttcacaaagc cattactagc
     9661 tttggtttcg gtcacataat aggttcccaa ttccaacgca ttggcaatct cttttggtgt
     9721 ttccgcatga cctttttcat ccgtggtcat ttcttggaca atttcaccgg ttggactgtc
     9781 tttgcgaatg gcaaacgtat ttccggctaa tgagtatttg tcgttccaaa gatcagttcc
     9841 tgtttccacc cccattttat tcaaaatgat ttgacctttt tcgcgagtat tttttgaagt
     9901 cacagaaata gtctcaccag atttaatggt tgcggtgatt ggtgtagtgt caatcgtata
     9961 aggagctggc actgattttt cagtaatggt tactttcgcg ccatgaggaa tctcatccaa
    10021 tgtcgcaatg ccttctttat cggtggtcac gtcttttgca ggtaatgttt ttccaaagtc
    10081 taaatggaaa acggttcccg gtacaacatt tcctgtttct ttatcgactt ttttaatctt
    10141 caaagaaccc tcagtttcca catcaatttt gattgtgtag gtattgggct tatcaattgc
    10201 ccccgccatc aaagtttgtt gaccagccat tttataagct accggtgttc ctgtattagc
    10261 tgacttcttt agggttaatt caccactctc ttttgaattg gcatttggag taatcactag
    10321 ttgattgccg ttcacacgat aatcaatatt tgcgatattc tcgacaactt catcaaactc
    10381 cgataaattc aagccgttcg tatctgtcac agtagtcgat tgacctaaca cggttttcac
    10441 tgtggagtta tggaagcttg gtttcttttg ataatccgca atggcttgat tgatttttga
    10501 ctcaatggct gcgatattta ctgcactacc atcagaacgt ttaattgagt gaagcgtgta
    10561 gccattaact tcttgccaaa tcatcttctg ggccacaata tgtgtatcca catccgtacc
    10621 agcttccttc cataaaacgg aaactaattt cgctttctcc gacatatcag gcaatggatt
    10681 tttctcataa ccaggagtga actcattgta aatcggcacc cctggctcaa tacagaaaat
    10741 atcttgcttc acaccattgt aaactgcata gtgcggaacg gtaacttctg acatgggacg
    10801 accatcgcta aatgtacctt cgacagcata ctggtgagcc aaatccaatt ccacagttac
    10861 cgtttgtgga tgagtaattt catcagcaaa ggcaatcgtt gggccaagcg atccaccaat
    10921 tgtttggcat aatagactca gtgttgcgag caaacgccaa ttcttaaatc ttggcttttt
    10981 cataaaataa atctcctttc gtttttgggt aataaaaaag acgtccaaat tttaattttg
    11041 aacgtctgat ttagagtgat actatttagt tttctttgtc ttggataggt tgtttctctg
    11101 cttataaatt ttgattcttc gataatcaaa tccaatctct gccatcaatt cctcccaatt
    11161 tttgttcgtt cttttcataa ttgtatcaac tgatggtagt tttggggatt gaactagttt
    11221 tcgaaacttt gtcattgata aatcatcctg tagttttaat cggattattt cattttttag
    11281 ttctaacagc agttcgaaat cagtaaatcg agatccatca tttttttctt taaaaagtgg
    11341 acgtatatct cctaacatct ttttaatggt actaagtgaa ggaatattct tccctactga
    11401 tttttgttcg tacattcgtt gagaggttat cttctcgcac ttaataaagg cttctactat
    11461 ttccaataat tcttgttctg acattctagc ccattttcca tacccagtat tttctcgtcc
    11521 tatactaatc aataaattct cccatgatcc atattttttg ttaatgaacc acatacttgg
    11581 tgcagaattt ggattttctg atagcttttt ctggtattga cggctagatg taattccgtg
    11641 tttttcgatg aattcattaa ttgttaaaat cagttttaac tcactttgat ttttccaaat
    11701 gatacgtcca tcattttgct tcacgataaa aactcctaat agttatttag atagaagcct
    11761 atcacatcaa ctttttagta gaaaagtaca tgttttgtct tttttgctaa tagctgaccc
    11821 tttgagaaac ttgggttcaa aagttgctaa attcggtaaa tgttttggta tttttcgtat
    11881 tatatttgtc gtcaagctga ctactaaatt ttgccctatt ttgacgacac tgatagtcaa
    11941 tgtcggacaa tctaggacaa tttttaaggg aatatgattg aaaaatggtc caaaacagcc
    12001 aaaaatggct taaaactgtg atagagaaaa aagaaaaagc ccttgatata caaggacttt
    12061 tcacaaattt cagatttcca aggaagtttt ccgtgtttat cttctgattt ctttgatacg
    12121 agcagctttt ccgtgtaatg cacgtaaaca aaaccactat attctattat gtatctaata
    12181 ccctcaaaac agctataaat caacaatacc gacatataat aaacactatg ttcaatctga
    12241 gatatatcaa aggtaaacgt atttttgccc cctttttgcc cctcaaatat cgctttacaa
    12301 cgagaacgta agttcgtata atcgttttga ggtg
//
LOCUS       DASJUF010000078        12316 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.078, whole genome shotgun sequence.
ACCESSION   DASJUF010000078 DASJUF010000000
VERSION     DASJUF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.078"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            375..932
                     /locus_tag="U4B62_002007"
                     /pseudo
     CDS             375..932
                     /locus_tag="U4B62_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            990..3029
                     /locus_tag="U4B62_002008"
     CDS             990..3029
                     /locus_tag="U4B62_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN2142154.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3138..4289)
                     /locus_tag="U4B62_002009"
     CDS             complement(3138..4289)
                     /locus_tag="U4B62_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN2142155.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4443..4973)
                     /locus_tag="U4B62_002010"
     CDS             complement(4443..4973)
                     /locus_tag="U4B62_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2142156.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5072..5422)
                     /locus_tag="U4B62_002011"
     CDS             complement(5072..5422)
                     /locus_tag="U4B62_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN2142157.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5579..7108)
                     /gene="ahpF"
                     /locus_tag="U4B62_002012"
     CDS             complement(5579..7108)
                     /gene="ahpF"
                     /locus_tag="U4B62_002012"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN2142158.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7118..7681)
                     /gene="ahpC"
                     /locus_tag="U4B62_002013"
     CDS             complement(7118..7681)
                     /gene="ahpC"
                     /locus_tag="U4B62_002013"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN2142159.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7825..8487)
                     /locus_tag="U4B62_002014"
     CDS             complement(7825..8487)
                     /locus_tag="U4B62_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2142160.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8671..10317
                     /locus_tag="U4B62_002015"
     CDS             8671..10317
                     /locus_tag="U4B62_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN2142161.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10308..11390
                     /gene="uxuA"
                     /locus_tag="U4B62_002016"
     CDS             10308..11390
                     /gene="uxuA"
                     /locus_tag="U4B62_002016"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2142162.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11498..>12316)
                     /locus_tag="U4B62_002017"
     CDS             complement(11498..>12316)
                     /locus_tag="U4B62_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEN2142163.1"
                     /translation="YIDFFIDLSVDILRPNLPICREQRILVNILARLSITKIESEETS
                     LEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFN
                     QPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVH
                     LYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCP
                     LKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         4112 a         2277 c         2319 g         3608 t
ORIGIN      
        1 tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag
       61 taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa
      121 agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg
      181 ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc
      241 ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga
      301 caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa
      361 gatcgattgg tggaggtcgc tcggattcac ttctggttgc acaatacaac cagaaaacac
      421 aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca ggttacggaa
      481 tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat caaacattaa
      541 aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa gattttatcg
      601 attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa tctctagatt
      661 tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact ttactccagt
      721 atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg cagcagcaag
      781 tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag ctacccaaag
      841 cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg cttctggatt
      901 gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata gtgaaataaa
      961 ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc
     1021 gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt ggcggttatt
     1081 tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag acagttgagc
     1141 aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg acatcaaaaa
     1201 aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agataaatac cagaatatat
     1261 acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat aaaaaagacg
     1321 attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt gaattgaaag
     1381 atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc aactggcaac
     1441 ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc acacaagaag
     1501 cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa
     1561 aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca gccaatatca
     1621 aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg atttcacaaa
     1681 gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca acgccagaac
     1741 ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg ggtgaagcag
     1801 ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat aagaatccag
     1861 aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt gataaggatc
     1921 ttcgtgggac aacaggtggg aaattaagca ttacagatgc agacggtgta gagaaaaaag
     1981 ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc gatgcgaagg
     2041 cacaaaaaat agcttttgac agtctaggag gaaaagctgc atcaactgtt gcgacaacgc
     2101 caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca aacaaaatga
     2161 caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa catccattca
     2221 tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaatgatt acagcagcaa
     2281 tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac gggcttaaat
     2341 ggcaaaaaga cagttcttgg ggatctttta aaacaacgcg tgtcagtagt gatgtatcac
     2401 aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca caagaaacgt
     2461 tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt ggtgaagacc
     2521 ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc tttaactcag
     2581 atatcttgct agctgatact ggatatggac agggcgaact tctaattact cctatccagc
     2641 aagcagcaat gtattctgtt tttatcaaca atggcacact tgtctatcct aaattgattg
     2701 cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta caaacgatcg
     2761 tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct ctttctgctt
     2821 tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa caggatgtaa
     2881 aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt tatatgatgg
     2941 tcagcatgtt ggaaaataaa gaagatgatg attcagcaac caaacgagca tcggaactat
     3001 tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag taaaaggact
     3061 gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt ctctttcaca
     3121 acttcttttt gatttactca cacttcataa actttttttg taaaatattt gagaacaagt
     3181 ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta ctgtggttcg
     3241 atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc
     3301 ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa
     3361 taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat aaagagtagt
     3421 tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa tactggtata
     3481 aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac ctttgtctga
     3541 ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac aagatccgct
     3601 acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga atttgcgtac
     3661 actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa gaaaataaaa
     3721 acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct agaagcttcc
     3781 cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc atctaccaca
     3841 gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc ttttaatact
     3901 tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat cgcttcattt
     3961 cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa gatgatccca
     4021 agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa atcactttcc
     4081 agaccagcta aaaacattaa taagatcaca ccaatctccg aaaagtcatg gactagaata
     4141 tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag taattgtcca
     4201 atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt tgaaatcaat
     4261 atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa aatatagccc
     4321 gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa aaaaaccaac
     4381 aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat aactaaaaat
     4441 gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg gatgcccatg
     4501 aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa tggattgaac
     4561 aataagacga atccgccaat caacaacaga atactactat aaacactcca acttttagct
     4621 tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc taatccaaga
     4681 agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag agctaagatc
     4741 aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct tttaaaacgg
     4801 taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat cagataaccg
     4861 ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat aatataaaca
     4921 gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa catataagaa
     4981 aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa tgttacaaaa
     5041 tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt tccatttttt
     5101 tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc atcaagttat
     5161 cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa cgtgaaggat
     5221 tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt
     5281 ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact tcctctttta
     5341 tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca aaacttgtcg
     5401 taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt atttactact
     5461 tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa aaggattgtg
     5521 acaaaattct tgtcaccatc cttttttccg ctctctgact ttttacaatc ttcatagctc
     5581 aagtgcgcat cagataatcg aatgctccta aagctgcagt agctcctgaa cccattgaaa
     5641 caatgatttg tttgtatgga ctatctgtac aatcaccagc tgcgaaaacg ccggggatat
     5701 tcgttgcgcc atttttctcg gtcactattt catttcttgg attcatttcg atcgttctat
     5761 ctagccaatc cgtattaggg agtaagccga ctagaatgaa tactcctgaa acagctagtg
     5821 tatgcatctc catcgtacgg cgatcgatat aggagagaga ttctacctga ttcgtaccgc
     5881 tgatttcttt cgtcgctgca tgtttgataa cagtgacatt cgcaagagag actagtttat
     5941 cctgtaagat ttgatctgct tttaattctg gtaagaattc tagaacatat acatgttttg
     6001 ctaacccagc caaatcgatt gctgcttcaa caccagaatt accaccaccg ataacagcaa
     6061 tctcttttcc tttaaataaa gggccgtcac agtgtgggca ataagcaatt cctttatttt
     6121 taaactcttt ttcgcctggt acattgatag aacgccagtg cgcacctgtc gcgatgatgg
     6181 cggtttttgc tttaagtgat ataccgttag ctaagccaat attaataaaa tgttgctctt
     6241 tatttaagga aacggcctgt tgattcatca taatgtcgat agggtaacta cgcacatgac
     6301 tttctacttg ctgcatcaat tttggacctt ctgtgtaaga ggtaccaatc atattttcaa
     6361 tccctagtgt ttcaacgact tgtccgccga aagaatcagc gacaagtcct gtacggattc
     6421 ctttacgtgc tgcatagata gctgcactac tagctgctgg tccaccgcca atcactaata
     6481 catcataagg ttctttttca aacaactctt cttcaatcaa agggccagtg attttttcaa
     6541 caatttgttc gatcgtcatg cgtccgctac taaattcttc tccatttaaa aacacagttg
     6601 gcactgccat gattttttta tcatcgattt ctttttggaa catcccacct tcaatcatcg
     6661 tatgagtaat atttggattt aatacagcta atatattcaa tgcctgtaca acgtcggggc
     6721 aattatgaca agtcaagcta acataactgt caaaatgcaa tgtttgtgtt atttttttga
     6781 ttttaagact caaagattca ctgattttcg gtgcccgtcc actaatttgt aacatagcca
     6841 gtaaaaaaga ggaaaattca tgaccgaggg ggataccagc aaaaacgatt ccactttctt
     6901 tgccaactgg attgatagaa aaactaggtg tgcgcatcga atctcttttt tcccaagaga
     6961 ttttaggaga catttctgct atttccttcg taaattcagc tagtttttga ctattttcac
     7021 tattatccaa actaacagaa aaaacaatag gagattcaat cagtgctaaa tattgttcga
     7081 gattttgttt agtttctttg tctaacatat ccaccaccta aatctttcca acaagatcaa
     7141 aacttggttt caatgtttcg ccactttctt tccattttgc cggacaaact tcacctgggt
     7201 gggtacgtac atattgagca gcgcgaatct tatcaatcag tgcactagcg tcacgaccga
     7261 ttccatctgc attgatttcc atagcttgaa cgattccgtc tggatcaata ataaatgttc
     7321 ctcgctgagc taatccttgc tcttcatcca aaacatcaaa aatacgagaa atctgatgag
     7381 acggatcacc gatcatcacg tattcgattt taccgatagc atctgatgta tcatgccatg
     7441 ctttgtgagt aaagtgggta tcggtagagc aagaaaagac ttcaacacct aaagatttca
     7501 atgtactgta ttgttcttgt aagtcttcta attcagtagg acaaacaaaa gtaaaatcag
     7561 ctggatagaa gcaaagaata ctccagttcc ccataagatc tttttcactg acttctaaaa
     7621 attctccttg atgataagca tgtgcattaa attcttcgat ttttttccca atcaatgaca
     7681 tagtaaaacc tccaataata attatttatt tataattatt gtaatttagt tggagttgca
     7741 aagtcaatgg atattttgtt atttaataaa gattactcaa aaacgaaggt taattataaa
     7801 aatagaagag gtcgcaagaa aatgctactg ctggaaatac tcgggataac gttgagtaac
     7861 agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc
     7921 tggtttttta tcaactaaag ccttgagtaa atcataatgt tgttccatga tcgtttccca
     7981 tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt tctaaatggg tattatggtc
     8041 atctaaccag ttccaaactt cttttcttcc tgcaatttcg aaacaagctt catgaaataa
     8101 attatctgca cggaaaaaag cgcgttcgtc atttattttg actgcttttt cttgttcttc
     8161 taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat
     8221 gatttgctct tccaactgtt ctcggacaaa gcgagcattt tctgcagaaa caagattaat
     8281 tttggaaaca tacgtaccac tttgaggtat cgtatacacg agttcttgct gacgtagttg
     8341 aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga tgttcctcca gtgccttttc
     8401 agatattttc cttccaggtt ctaagtcagc atagataatt tgtttacgaa tcgcttgata
     8461 agcttgtgat tgcaagctgc ttttcatctt tattcctcca aaatcatttc tatgaacgta
     8521 gtataacaga agaggtctga aaaacaatct ggtatgtcac attaactagt aaatttgtat
     8581 aaatagtaaa attttggttg acatagcgat ttttattgca taaaattcac ttgaatacaa
     8641 aaactaatat gttagtttag gaggggaaaa atgattgatt taaacaactg gaggaacttt
     8701 atccctgaat tatctcaaaa agaagtagaa atacctcatt ttgaccgtgc atcattaaga
     8761 gaaaatggac aaaaaaatcc tgtctggatc catttcggtg gcggaaatct ttatcgagga
     8821 tttcatgcag aaatcgctca acgtttagcg gatctaggcg aattgtccac tggtgtcatt
     8881 gtttgtgaaa cattcgatga agaaataatt gaaagggctt accaagattt tgatgatttg
     8941 attcttgaag tggtcatgcc aagcagtgga gagttcgtca aacgtatatt agctgcgaca
     9001 gctgacagtc tgtattgtca tccagattgt tctgaacact ttgataagat gattcgacac
     9061 tttgaaaatc ctagtttaca attagttact tttgctatta ctgaaaaagg ttatcgattg
     9121 aaagatacaa ataataaata tataggaccg attgcagtcg atataaaaaa tggtccaaag
     9181 aagccatcac atacaatgag tattcttgct gtgctacttt ataaacgttt tttatctgga
     9241 gcttggccga ttgcacttgt ctctacagat aatttttcac aaaacggtag tcaattacgt
     9301 acttctttgc taacgatcgc aaaagaatgg caaaaggaag gttatgtaca aaatacattt
     9361 atccaatact tatcgaatga agaaattgtt gcgtttccat ggacgatgat tgatcgtatc
     9421 accccgaatc cttccttgtc agttagcaaa atgttggcaa aagagggctt ttcaaatatg
     9481 tcgattttcc aaaccgcaaa aggaaataat attgctccat ttgtcaatac agaggacatc
     9541 cattatttaa tcatggaaga tcattttccc aatggacgtc cgaaattcga aaaagctggg
     9601 gtattcttga ctgatcgtgc aacggtagat aaagcagata cgatgaaggt gacgacttgc
     9661 cttaatcctt tgcatacgtc tttagctatt atgggttgtt tactggggta taggagcata
     9721 gctgaggaga tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa
     9781 ggcctcccag ttgtggagtc tccaggtatc attgatccaa agatctttct ggaagaagta
     9841 attactgtgc gcttttctaa tccaaatatt cctaatatgc ctcagcgaat cgccacggat
     9901 acctctcaaa aggtggcgat tcgttatggt gagacgatta aaaaatatat ggaaatagaa
     9961 gttaaagacc cgcagacact tgtattcata ccattgactc tcgctgcttg gcttcgatac
    10021 cttattggca ttgatgatca aggaaaacca ttcatcccga gtccagaccc actattgaca
    10081 gaccttcagc ataagctttc cgatattcaa ctgggtacgc aagatacatc acatattcat
    10141 gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg gtgtagatct ttaccaagca
    10201 gagcttggca gaaaaataga agaaatgttc atcaagatgg tagcaggacc aaatgccgta
    10261 cgaaaaacat tacaggatta cttacaagaa catggaggaa atttatcatg gaaatgactt
    10321 ttagatggta cggatcacag gaagaaaaga ttaccttaga tgaaatcaga cagattcctg
    10381 cggtcaaagg gattgtaggc acattgatgg atataccagt aggagaggta tggcctaggg
    10441 agaggatcaa agaactaaaa gaagaaattg agtctcgtgg gttaacacta aaagtaattg
    10501 aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc ttcccgtgag cagtatatcg
    10561 aaaactataa gcagacaatc cgaaacttgg cagaatatgg aatagaagtc atctgctata
    10621 attttatgcc tgtatttgac tgggtgaaat ctgaccttga ctaccgctta gaagacggct
    10681 catccacgtt agcttttata tctgatgata ttccagatga tccaagagaa atcgtcgcgc
    10741 gcatcgaaca ttccagcaat gaatttggat tgccgggatg ggaacctgag cgcttagctc
    10801 ctatcaaatc cttgcttgaa gcctatgcat cagtagacga agaaaaactt cgagaaaatt
    10861 ttgcctattt tctccaatca atcatcccaa catgtgaaga ggtaggggtc aaaatggctg
    10921 tacatccaga tgatccgcca tactcgattt ttggattagc tcgaatcgta aagaatcgag
    10981 aagatttaga ttggttgtgt cgcatagtag acagccctta taacggaatc actctttgta
    11041 cagggagtat tgcagaagat ccagaaaatg atgtatatgg gattttagca gaattttgcc
    11101 aaagagatcg aatcccattt gcacatgttc gaaatattaa atttttagct aatcgagatt
    11161 tctatgaggc accacataaa tccgaatacg gttcattaga catgtaccgc atactaaaaa
    11221 caatgtatga caacggattt aacggataca tccgtcctga tcatggaaga atgatctgga
    11281 atgagacagg aagaccagga tacggcttat atgatcgagc attaggtgct gtttatctaa
    11341 atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac
    11401 tgtaatttta ttatgtaaat aagaaggatg ttcaaattct tgatacataa atccggaggt
    11461 tgttaataag cattcggatt tatgtatctt atttggtcta ggaagtaact tgattttctt
    11521 ttttcgattg atccatgagt gcttcttctt ttaattcaaa gatatattct gagatcctca
    11581 taatcacttt tttccagtct tctggtgtga tgattccttt taaaatatag atttttttga
    11641 tatgagaagg caattgtttc aaagggcagt cgctgataac gatgttgcag ttctccataa
    11701 aatcattttg tattttgatc gattcatttg aaaaaatttg ttctatctta acgatcagat
    11761 ctttactgat ataaaaatca cgcgtatagt tcacaccaat cacaattcgc ggctgttttt
    11821 tgtagcgatc atacaaatgt actaggtctt caaccatcca ttgcacattt tccttttcta
    11881 aaataactgg taacaagtgt tgattttgtt ctctgaaatc catataaaat tgttcaatgg
    11941 cggtttggac ttctttcaat acgctatgtt cttgataaag catgaaagta ggaaggggcg
    12001 tatttggttg attgaagtta agaaatccat tataaagttt atttcgtgcg aatgccaatg
    12061 agtaaagaat cttttctttt tcaggtataa acaaatcaaa tacagtacaa agggactcca
    12121 ccatttctct tgaataaaga acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct
    12181 ccaagcttgt ttcttccgat tcgattttcg tgatagaaag tcgagctaaa atattcacta
    12241 agatgcgttg ttcgcgacaa attggcagat taggtcgcaa aatatctaca gagagatcaa
    12301 taaaaaagtc aatata
//
LOCUS       DASJUF010000079        12233 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJUF010000079 DASJUF010000000
VERSION     DASJUF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.079"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..161
                     /locus_tag="U4B62_002018"
     CDS             12..161
                     /locus_tag="U4B62_002018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142164.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            complement(195..359)
                     /locus_tag="U4B62_002019"
     CDS             complement(195..359)
                     /locus_tag="U4B62_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142165.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            654..1982
                     /locus_tag="U4B62_002020"
     CDS             654..1982
                     /locus_tag="U4B62_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN2142166.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2184..3017
                     /locus_tag="U4B62_002021"
     CDS             2184..3017
                     /locus_tag="U4B62_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN2142167.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3033..3770
                     /locus_tag="U4B62_002022"
     CDS             3033..3770
                     /locus_tag="U4B62_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN2142168.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3925..4893)
                     /locus_tag="U4B62_002023"
     CDS             complement(3925..4893)
                     /locus_tag="U4B62_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN2142169.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5125..5958
                     /locus_tag="U4B62_002024"
     CDS             5125..5958
                     /locus_tag="U4B62_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN2142170.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5992..6831
                     /locus_tag="U4B62_002025"
     CDS             5992..6831
                     /locus_tag="U4B62_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2142171.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6857..7417
                     /locus_tag="U4B62_002026"
     CDS             6857..7417
                     /locus_tag="U4B62_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2142172.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7616..9337
                     /locus_tag="U4B62_002027"
     CDS             7616..9337
                     /locus_tag="U4B62_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN2142173.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9502..10644
                     /locus_tag="U4B62_002028"
     CDS             9502..10644
                     /locus_tag="U4B62_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2142174.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10660..11871
                     /gene="thiI"
                     /locus_tag="U4B62_002029"
     CDS             10660..11871
                     /gene="thiI"
                     /locus_tag="U4B62_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN2142175.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4163 a         2053 c         2545 g         3472 t
ORIGIN      
        1 ggtagtagcg catgactgtt aatcatgatg tcgtaggttc gagtcctact gccggagtaa
       61 ataaagctta tgcaaagaaa aagaaatcca atcacttggg tttctttttc ttttattatt
      121 atgatgaatc aagctttctg tttttgtcac gtatgttgta atctttacga aagagtagag
      181 ttggctactc ataatcaacc atacttccca tcatcgctaa atacgaaata aatcagcatt
      241 cctacaccaa tcaaagcaca agctgcacca agaaaaggga tgacattccc aaagttatct
      301 gtattcataa gtatgccacc acccacaaac aaaaagccgc cagcaatcag gtttttcatt
      361 ttattcatct cctttcttac gttatagtat ttccttcttt caatgagtat tttgaaagct
      421 atcttattgt atgatgttct tctaggcctc ttctttatta tacaggccat tttcgttatc
      481 tcatagaaat attccttttt atctgaaaac tattgtatac cgtatgacat ttttcttcat
      541 gaagttgtaa gtatattctc attcaaatga aaatttcatc atagaagaga ttgtatttct
      601 aaattcttaa gagtaaaata cgaagtataa cttggatttg gaaggatgat tggatgaaag
      661 ctattacgcc tgatatgaca acaaaattta acaaacagtt tcaagcaaat atcaaacagc
      721 aggcattgca gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa aatgtttcag
      781 cacatgtgga gaatacaccg gtattttcta tagatttggc tacggggaaa gtggctaatc
      841 aaatgcaatc ggggcgttgc tggatgtttg ctgcactgaa cacattccgt cataagatgc
      901 tgaatcaatt ccatttaaaa gagtttgaat tatcacaaaa ttacactttt ttctgggata
      961 aatatgaaaa ggcaaattat ttctatgaaa acatcattgc aacaggaaaa gaggcaatcg
     1021 atagccggaa agtagctttc ttactcgcta ctcctcaaca agatggcggc caatgggata
     1081 tgatcgtttc attattccaa aaatatggcg ttgtgcctaa atcggtgatg ccggaaagtg
     1141 cgaatagttc aaattctcga gatttgaata actacttgaa caaattactg cgtaaagatg
     1201 cagtagtgtt acggaaaatg gttgctcaag gagaaacatt agacgctatt gaagaaaaga
     1261 aagaagaaat gctagaaagc atttataact tcttagctat ctcattagga acacctccaa
     1321 aagaattcga ttttgagtac cgcgatgaag aaaagaacta tcatcttgac cgaggactga
     1381 caccacaaat tttttatgat aaatacatcg gtgtaaaatt agatgattat gttagcgtga
     1441 tcaatgcacc aacaaaagac aaaccattca atcgttctta tacagtagag atgctaggaa
     1501 atgtcgttgg cgggaaagaa gtcaaatatc tgaatgttga tatggaaact ttcaaaaaat
     1561 tagcgattgc tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat gtaggtcaat
     1621 cttctactag aactagtggc atcatggcct tagatgctta ttcaatggat gatctatttg
     1681 atacggattt tacaatgact aaagctgaac gtttagattt tggagaaagt ctgatgaccc
     1741 atgcgatggt attgaccggt gttgacttag tagacgggca atcaacaaaa tggaaagtcg
     1801 aaaatagctg gggcgaaaaa gttggaaaga atggcttctt tgttatgagt gatgcctgga
     1861 tggacgaata tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa gagcaattaa
     1921 atgcgttcaa tgaagaacca attgttctat ctccgtggga tccaatgggt gctttagcat
     1981 agacgagtat ttcaatgaag agtatgtagt aaagataaaa agagctggct taagccagct
     2041 ctttttatct ttaaagtcca ttccctgttt tttatggtcc ctgtgctata attctaaata
     2101 aaggataagg attggaaata atccttttat cattaaaaat atgatgacga agtagaataa
     2161 aattgggagt aggggggaaa gtcatggata tccaaactgt tttatctgat ctgaaattaa
     2221 atggtaaagt gattccagtt gttggtggcg atgttaatca gacctatcgt ataaaaaccg
     2281 aacatcgtgc ttatttttta aaaatccatc cgaatgtaaa aaaaggattt tttgaagcag
     2341 aagtagacgg attaaaagaa ctgtcagcat tcgtacgtgt accggatacg tatatgctag
     2401 gtgaaacaag tgaaggcgct tatttgttaa tggaatggat cgagccggga aaaggggatc
     2461 aacgagatct tgctgcagct ttagctaatc tccatcagca aacagcgcca caatttggtt
     2521 ttcgtaaaga taactatttg ggtacacttg ttcagaaaaa ttcttttgaa gaagattggt
     2581 ggactttttt ctttaaagat cggcttgaat cccagatttc tttggcagaa gaaacgaatc
     2641 gatggaatgt acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta ttgaaatcgg
     2701 ttgaaccaaa aaagatcact ccacgcctct tgcacggaga cctgtggagt ggtaatgtgt
     2761 tctttgacca gcaagggcat ccagtttttg tcgacccagc tgtttcttat gggaatcggg
     2821 aacaagatat tgcaatgagc cagttattcg gtggtttccg accggaattt ttagatgctt
     2881 atcaaactat atttccatta gaaaagggat ggaaagaccg actgccgatt taccagttat
     2941 attatctttt ggcacatttg aatatgtttg gagaaagtta cggttcccaa gtagatcaat
     3001 tactagaaaa tttttaagag gagggcgtgt ttttggcaaa aaaatatacc aaaaagcatg
     3061 aagtttcata ttatgagtgt gacatcaacc aaacgatgac atttccttcg atgttaggag
     3121 tagttatcaa aacgtctgaa gatcaaagcg accagttggg aagaggcagt gaattcgtca
     3181 atagctttgg acttacttgg gtcatcacga attattccat ggagattaat cgtctcccta
     3241 gagtcggtga agtaatctca gtaacgactc aagccatgga gtacaataag tttttctgtt
     3301 atcggaactt ctggatccat gatgaagatg ggaaagaatt agtcaaaata gaatctgttt
     3361 tcgtattgat ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag atcattgctc
     3421 cttttgagag tgagaagatc aagaaaatca agcgtcagga aaaaattgaa aagattgatt
     3481 cgggcttcat gttgccatat cgtgttcgtt tttatgatat cgatagtaat cagcatgtta
     3541 ataatgcgat gtattttaat tggatcattg acgtattagg atatgacttt ttgactacgc
     3601 atgttccaga aaaagtaatt attcgatttg ataaagaagt agagtacggg aatgagatcg
     3661 aaagccattt tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat gaaatccgaa
     3721 tgcaagaaca gctgtattgt gaagcaaata ttatatggaa aaataaataa aaaagaggtt
     3781 gtgacagaag ggggcaactt caagaaataa gaagaaattt acgaaaattg tttctcaaat
     3841 ttttgtaaat tttcgactta gttttcgaag aagctatttt tgtcacagcc tctcttttta
     3901 agtttagcta ttagataatg atgtttagtt ttggataaac gaaatcaatt cttcacccga
     3961 caaaccatag tttacagcaa tcaatagttt taaacgtgct ttttgactat taatactgtt
     4021 acagaaaata atccccattt tttctagttc ttttcccccg ccttcataat cataaacagg
     4081 ctctgcgatt ccattgaaac aacgagaaac aagtactaca gggatttttt ggacaagaag
     4141 cttttccaaa gcagctaagg cttgtggagg gagattcccc gcacctaaag cttcgattac
     4201 caaaccgtct actttcgtat gggcgatggc ttccaataag tccccttgca tcccagcata
     4261 tgctttaacg atcgggatag tcccatctac agcggaaatg tccagtcgct ttgtttctaa
     4321 aagttcctgc aagaataaaa ttcgattctt agttactaag ccgatgggac ccaatgtagg
     4381 tgttcggaaa gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg cagaatggat
     4441 ctcatcgttc atgaccacca aaaccccctt atctagcgct tcttcacaac ttgcgacacg
     4501 aatcgcactt tcaaaattat aaaggccgtc acttccaagt tcattacttg aacgcattgc
     4561 cccagtcaaa acaattggta atttgttgcc aatcgttgta tcaagaaaaa aagcagtttc
     4621 ttctaatgta tctgtaccgt gtgtgatcac cactccggat atacctgatg cataagcgga
     4681 caagatacgt tcttttaata ccaacatgtg agaaggcatg atgtgtggtg agggaagatt
     4741 gaagatatct tcaactaata attctacatg cttggggatt gtgatttctg catctaataa
     4801 tgggtttgcc acatcaggac taacaccgcc cgtgagcatg ttttctttca tggcgatcgt
     4861 tccgccggtg tgtagtacaa gtattttttt cattttacag acttccttac gaataatttt
     4921 gcatagccgt taaatcttat tgtatactat ataaaagagt tttgctatca gtgaagaata
     4981 acgaatagcc taacttatca taaaaaagct gaccttatta aaatggaaaa gactcaacct
     5041 tgacctccta atacaatgga gtatctggtt gagtttcttt tacaagaaag cagccaagga
     5101 aatcaatcac gaaggtggcc ggaaatgatc agaatcggat taacatcgtt taaagaacat
     5161 gacaaattaa ctggtaaaaa gaataacact ctttatgaat atgcagggtt tctaccctta
     5221 gtggaattag atactgccta ttatggaatt ccccgaaagt cctcagttga gaattggata
     5281 agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat cagcggtcaa
     5341 ggagactgga agccttatta tcagaccgaa gaagagatga ttgcgcattt tttagatgtt
     5401 atgtcgccaa tggtagatgc tggaaagatg ttttgctttt tagtccaatt cccctcatca
     5461 ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg gtttgagggt
     5521 tatcctgttt ctatagaatt gcgggattat tcttggtaca gcgatgaatt tcttgaacgt
     5581 acaagaaact ttatgtctag agccgctttt tctttagcca tcatcgatga gccacagttg
     5641 ctgaatacaa cgattccttt tgatccattt gtcaccaatc cgaactttgc tctttttcga
     5701 ttccatggta ggaatgcgca aggatggcaa gcaaatgata aagagtggcg taaaaaaaga
     5761 acgttatatc gttattcaga aaaagaactg caagaactag cacatgaagt caaagtagtt
     5821 gattctaaga caaaagaggt aggtgtgatt tttaataata attctggtgg tgatgcggct
     5881 gaaaatgcat tagcactgca aaaaatatta aatatcaagc cagatggact caatccaacc
     5941 caaatggatc tgttttaagg gcttatttta ttcaagaaag gggcgaagtc tatgatcaaa
     6001 gctatttttt ttgatattga cggaacattg gtcaccaatc gttcaaaagc gttggaatca
     6061 acaaaacaag caattgaata tgctcggaaa aacggcattt tatgcggtgt agcaacgggg
     6121 cgcagcccag taaagatcaa agaaatcatt gatgaattgg aattagatat gtatgtcgtg
     6181 tataatggtc aactggtttt tactgcagat cgcacgatta tcgaccaccc ttttgaacaa
     6241 aaagtattgg aacatattgt tgaatttgca gatgagaacc atcgacaaat cgtttttggt
     6301 gcgagaaatc ggttagatgg atcaacgacg atgctattag gacaatcgat ttttatcaag
     6361 cgactagtca gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa aatcctccag
     6421 gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt aaaagaacca
     6481 atttatcaat gtatattgct cagtccggag tcagaacaga aaaagttaga acatcgcttt
     6541 cccgagtgta cgtttcagcg ttcaaatacg tatactgtag atatcatccc aaaaggtggt
     6601 tcaaaattac taggcatcca agctttcgca aatgctgtcg aaattgaaat ggaagaaatt
     6661 atggcattcg gcgatcatta caatgatatc gagatgctga aaggtgtagg aattggagtg
     6721 gccatgggga atgctcagat cgaagtaaaa catgctgcag actttgtgac tcaatcaaat
     6781 gaagaagatg ggatatacga aggactgaaa cacttcggtc tgatttatta gtcaatgaaa
     6841 gaaggaaaaa atcagtatgg aagcagagac accttttcaa aaagtagaaa cattccacaa
     6901 aacttttgat ccaagaagac cagagatacc cacggctttt acagaaaatg aagctcatac
     6961 acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt gcagctactt cacctaataa
     7021 agaacaactg catgaagctg ttatgctgct gcacgaagca ttagataaag cagaagaaaa
     7081 aatacttggt aaaacacagt ggggaagtga tccgctggtt gaacaagtag atgcactgac
     7141 agatatcctt tactttactt atggttcttt ttcattacta ggaatagtgc ctgatcgaat
     7201 attcgaaatc gtccatcagg caaatatggg gaaattattt ccagatggaa aacctcatta
     7261 tgaccctgta acaaataaag tgttgaaacc tgaaaactgg cagatggaat atgctccaga
     7321 acctaaaatc gcggcagaaa tccaacgtca gattcaagaa gcgattgcta agaagaaatc
     7381 cgctgatata ggcatgtgca caaatgaaaa agagtgatca gagtttcact atttttgtta
     7441 tctttatacg atggaaaaac aaaagatttt cgtttaagtt agtgaatgca tgaaaatagt
     7501 atgctataat ctagaataga tgtcttttgc acgagagacg tattatttgg atttatgcca
     7561 agcagaacgc catctgttgc ataaaagatg catactgaaa acggaggaac cataaatggg
     7621 aaataatgtt atcatcggaa tcgtcattgc aatcattata gtcgccattg tcgtcttttt
     7681 ggttagttac ttgatgaaga aaaaaaacca agatcgattg gatgccttag aaaaacgcaa
     7741 ggaagaatta tttgacttgc cagtgattga agaagtcgat gatgttaaaa aaatgcatct
     7801 ggttgggcaa agccaaaata cctttcgtga atggagtcaa aaatggaatg acatatctac
     7861 ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg gaaaacctca atgaaagttt
     7921 tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg accaaatgga
     7981 gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg aagaacgcaa
     8041 ctcactagct gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa aagtcagaga
     8101 agacgagcat gaatacggac cagctctttt tgaattgcaa aaacagatca aagtgatcga
     8161 aaaagaattt acgcagtttg tgactttaaa cacttctgga gaccctgttg aagcccgcga
     8221 ggttttggat aaagcagaaa aacatacgta tgaagtggaa gctctgatga aaaaaattcc
     8281 accgctgtat gaggagctcc ataccacttt ccctgaacaa cttgaagaaa tcagtgatac
     8341 ttacgagaaa ttgatggaag aacaatatgt attcccagaa gaagatcttg ctgaagatat
     8401 cgcaaaagta agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa cagaagttga
     8461 aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt gattcgttat atgatatctt
     8521 agaacgggaa atggaagctc agagatatgt aaaaactaac caatcaacga ttgcagagta
     8581 tatcaaacat acaacaaaga ataaccgtca attattgatt gagctggatc acacatctca
     8641 aagctataca ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa ccgaagtgga
     8701 agaaatggaa cgtcaaaatg aacagatgat tccacagatc cgtcaacacg aaattcctta
     8761 ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc ttagaagata tcgaaaagca
     8821 acaagtagag atcgacgact cattacatga attgagaaaa ggcgaaaaag aagcgcaaga
     8881 aaagatcgat acattcgaat tcaaattgcg cagtttgaaa cgttttgtag aaaaacaacg
     8941 tttgcctggc ttgccgaatg attacctaga attctttttt gttgctactg accgtattga
     9001 agaactaagt gttgtcttga ataaaattcg agtaaacatg gaagaggtca atcgtttagt
     9061 tgctctatgt gaagaagatc tagaattatt agataagaag acacatgatt tagtagatgc
     9121 agcagcatta acggagcaaa tgctccaata tgcaaatcgt tatcgtcata cacacgaaga
     9181 agtgagagaa gcaattgata aaagctacta tttattcaat aaagaatatc actatcaaga
     9241 agcattagac gaaatcggta cagcattaga gcgagtagaa cctggtgctt tcaaacgcat
     9301 cgaagatttt tattttaatc atccagactt agtataaagc aaaaaagtta gtaaaccagc
     9361 aagagcaaga cggattattg tcttgttctt gtcttttttt gtatatttga aaattgaaac
     9421 aagtgaaaat ttcacctgct cttgaaagca ccttggacaa ccgttataat agaaaagaat
     9481 ttcaaaaaag aggggtttct tatgatatat tttgataaca gtgcaacaac cccaatcttt
     9541 ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga ttattgggaa tccatcaagc
     9601 ttgcatgatt taggcaatca agctaaccgg ttgttgcagc aagcaagaaa gcagatcgct
     9661 gatttgatcc atgtagaacc agaagaaatc tattttactt caggcggaac agaaggagat
     9721 aactgggtat taaaaggaac gatgatcgaa aaaagagaat atgggaatca tatgatcatt
     9781 tcaggagtcg aacatccggc cgtcagcgag acagcagaac aattaaaaga attaggaatc
     9841 gaagtaagta tcgccccagt ggataaacgc ggatttgtac gtgtcgacga attgaaagaa
     9901 ttaataagaa aagagacagt attagtctct gttatggctg tgaataatga agtaggatcg
     9961 attcagccca tccaggaaat cagtgatctt ttggatgatt ttcctaaaat tcatttccat
    10021 gtagatgctg ttcaggcgat tggaaaagta ccattctcgc aatggttgac agaaagagtt
    10081 gattttgcaa cattttcagc tcataaattc catggcccac ggggaactgg tttcatttat
    10141 tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga aagaaatcag
    10201 cgaagtggga cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt acgtttatca
    10261 ttagagataa aggaacaaaa acctgagcag acacaacgct taaaagtagc tctaatcaat
    10321 gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag gaactgacta tgcaccgcat
    10381 attttatgtt ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc cttagaagaa
    10441 aaacaaatat ttgtttctac tactagtgcc tgctcctcac gtaaacatat ggcaagcagt
    10501 acattgcacg caatgcatgt accaaatgat ctggctacat ctgctgttcg aatcagctta
    10561 gatgaaaaca attcgcttgc agaagtcgag caatttatga ttattttcaa gcagctttat
    10621 aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag tgaaattatg
    10681 gtccgttatg gggaactttc gacaaaagga aagaatcgaa aaagctttat catgcaacta
    10741 gcacaaaatg tcagacaagc tttgaaagac tttccagaaa tcaaaatcca tgctgatcgg
    10801 gaccggatgc atcttttatt gaatggcgca gacgggcagc tagttattcc aaaactagca
    10861 aaaatatttg ggatccaaaa tttctctcca agtattcgtg tagaaaaaga tgtcgaagtt
    10921 ttgaaacaag ctgttcaaga catcatgaaa gaaatttata ctgggaaaga aacatttaaa
    10981 attatcgcta aaagaagcga tcaccaattt gaattgacaa gtaatgaatt gaatcaaaca
    11041 ttaggtaatg ctgtttttga tattttcccg catatccaag ttcaaatgcg tcaaccagat
    11101 attccattac gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga aacgatcaag
    11161 ggagctggag gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct ttctgggggg
    11221 attgattctc ctgtagcagg gtactttgcc atgaagcggg gagtggaaat tgaagctgtt
    11281 cattttgcta gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa agatctaaca
    11341 gccaaactag caccttatgt gggaacgatc cattttatcg aagtgccatt taccgagatt
    11401 caagaagaaa tcaaaaagtc agtgccacaa ggatattgga tgacaattac tcgtcgaatg
    11461 atgttgcgtt tgaccgatga aattcgccgt attcgtcatg gtttagtcat tttgaatggt
    11521 gaatcattag gtcaagtggc ttcacaaact ttgcaaagta tggtggctat caatgaagta
    11581 acgaatacgc caatcattcg gcctgtagcg acaatggata aacttgaaat cattgaagta
    11641 gcagaacaga tcgatacatt taatctttct atccagcctt ttgaagattg ctgtacgatt
    11701 tttgctcctc cacagccaaa aacacgccct caactggaaa aggtccatca atatgaagag
    11761 cgtttagcaa tagatgaaat gatccaacga gcactagctg gcttgaagat ggaagagata
    11821 caagcagaat cgtctaatga aaaaactgaa gaattttcag aattgctata agaggttatg
    11881 aaaatagtgt tttgaccaga gtattagaga aacaaaacga aaaaatagct ccatgtattt
    11941 taccgttttt gaactaatgt cgaagattag ctgattgaat accatttatc aagaagttat
    12001 ggaaaagccg gttctttttt actaactgaa aaataaaatt ttatttagag atcaagctac
    12061 agttgataaa tgatataaaa aagagaattt cccatgaaat agcgaggttg tgacagaagt
    12121 gggcagcttc aagaaataag aaggaattta cgaaaattgc ttttcaaatt tttgtgaaat
    12181 ttcggcttat ttttcgaaga agttacttct ggaacaccgt gtatcagagg ttg
//
LOCUS       DASJUF010000080        12185 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJUF010000080 DASJUF010000000
VERSION     DASJUF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.080"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(354..1472)
                     /locus_tag="U4B62_002030"
     CDS             complement(354..1472)
                     /locus_tag="U4B62_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142176.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1943..3250
                     /locus_tag="U4B62_002031"
     CDS             1943..3250
                     /locus_tag="U4B62_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN2142177.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3369..4568
                     /locus_tag="U4B62_002032"
     CDS             3369..4568
                     /locus_tag="U4B62_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2142178.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4595..5863)
                     /locus_tag="U4B62_002033"
     CDS             complement(4595..5863)
                     /locus_tag="U4B62_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN2142179.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6026..6672)
                     /locus_tag="U4B62_002034"
                     /pseudo
     CDS             complement(6026..6672)
                     /locus_tag="U4B62_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7080..7583)
                     /locus_tag="U4B62_002035"
     CDS             complement(7080..7583)
                     /locus_tag="U4B62_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2142180.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7706..8470)
                     /locus_tag="U4B62_002036"
     CDS             complement(7706..8470)
                     /locus_tag="U4B62_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN2142181.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8578..8853
                     /locus_tag="U4B62_002037"
     CDS             8578..8853
                     /locus_tag="U4B62_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN2142182.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9291..10241
                     /gene="yidC"
                     /locus_tag="U4B62_002038"
     CDS             9291..10241
                     /gene="yidC"
                     /locus_tag="U4B62_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2142183.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10420..10620)
                     /locus_tag="U4B62_002039"
     CDS             complement(10420..10620)
                     /locus_tag="U4B62_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142184.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11425..>12185
                     /locus_tag="U4B62_002040"
     CDS             11425..>12185
                     /locus_tag="U4B62_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEN2142185.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRP"
BASE COUNT         3707 a         2542 c         2153 g         3783 t
ORIGIN      
        1 tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag aaataatttt
       61 tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct tccttaaacg
      121 gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat gaagagctat
      181 ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct ctcatacacg
      241 gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg tgaaactatt
      301 tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct cttttaataa
      361 cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa aataaacact
      421 ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg ccgatcatat
      481 tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat aaataaatgt
      541 gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat gaatttctta
      601 tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga cagcttcttc
      661 gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat cgcaacgtct
      721 gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc tggtaatgca
      781 aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta cgtatcaaca
      841 gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt tacgccatgg
      901 aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa accgccacct
      961 gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg tagagatact
     1021 tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt caatcccaat
     1081 aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata aggaagatca
     1141 cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt ctggtcagca
     1201 gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc tcctaacaaa
     1261 ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt ctcaaaacgt
     1321 ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac atcttgatgg
     1381 gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc tgaagtcaat
     1441 tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa aaaatcattc
     1501 ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa acgtatctaa
     1561 aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta tcttcataat
     1621 ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga taaactaaga
     1681 aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt attaagtatt
     1741 atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca gcctattcct
     1801 gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat cctaaagttt
     1861 tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt agcgaaacaa
     1921 attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc tattattggc
     1981 tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag ctgaagatac
     2041 gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta agattcttta
     2101 tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca tcggtcttta
     2161 catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag tttcgatttc
     2221 agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt tgcataaaga
     2281 aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag ccaatgcttc
     2341 aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg aacgaatgaa
     2401 agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat caggattgaa
     2461 caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg aaaatcaaat
     2521 gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc cagaaatact
     2581 ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac cagtggaaat
     2641 ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg tggacggatt
     2701 aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca ctaaaaacga
     2761 tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc cgagtgcccg
     2821 cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag aaaaagagct
     2881 cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag acggaaaaga
     2941 atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa acgatatgga
     3001 tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg acaatgagat
     3061 ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct tggcagacga
     3121 taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta cggataaaac
     3181 agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat ttttcggcaa
     3241 cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt tttttgcttt
     3301 ttctagttta aataacagct tttttcatct attttcgtta tactgtataa gaaaaaggag
     3361 ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt attggggtat
     3421 tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt tacggcagta
     3481 cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt tattcgacct
     3541 tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat ttcacagaaa
     3601 aacgaaggct actgaatggt cttttattca atgttttact catcagtttc ggcatctact
     3661 tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc gccttgatca
     3721 cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt tttctttttt
     3781 ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt ttaacgttga
     3841 tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta aaatatttgc
     3901 cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat ctatttatcg
     3961 tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct cgatacaatc
     4021 aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt tctcctcttc
     4081 tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt gctacattaa
     4141 atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta ccagaggcca
     4201 ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt cttgtcgaaa
     4261 ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg gatcagcaaa
     4321 tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt agagccggtc
     4381 tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca gctttctact
     4441 acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac aaaaaaagac
     4501 atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg attactttac
     4561 ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt tctttcattc
     4621 tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga gcagaataaa
     4681 taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag tatactgccg
     4741 cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta tttgtcgcac
     4801 cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca tggagcaaag
     4861 gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact tctccccctt
     4921 gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa tcatagacat
     4981 gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga taacgttgtg
     5041 tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt cgacaaacag
     5101 gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg ctgaataccc
     5161 aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc aacatactcc
     5221 attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt tctgtcatga
     5281 aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg actttcccga
     5341 ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat acagaactaa
     5401 aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc aatcggaaca
     5461 aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct tcacggccaa
     5521 cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga ataagccgca
     5581 acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt ccacgactag
     5641 aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact ggtagtagac
     5701 aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta tttaagaaaa
     5761 aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc cacttcagca
     5821 tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat tcctcctcaa
     5881 taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg tcattagcta
     5941 tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta aaagatagca
     6001 tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca gcaaattcat
     6061 agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga caaatataat
     6121 tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaaa
     6181 cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt gataaagtac
     6241 cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc tgcgtacctg
     6301 gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa gctggttgtt
     6361 tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc tgcataaacg
     6421 gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt gtcagtttta
     6481 cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta atatgtctcg
     6541 aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat tgacagatga
     6601 gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt atggttttat
     6661 tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa
     6721 agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa ataagatacg
     6781 ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg attcattcga
     6841 taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa aaaggactgt
     6901 gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag ctggcatctg
     6961 cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
     7021 ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg ttttatctat
     7081 taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact tgctgacata
     7141 ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat atatccttct
     7201 ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat atctcgctca
     7261 agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg atggatatac
     7321 gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag atcatgcaaa
     7381 agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag cagataacta
     7441 tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg tgtgtaattc
     7501 gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat atattcttcg
     7561 tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat ttcattttct
     7621 tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc ttggataaaa
     7681 tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca atccctgccg
     7741 caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg ttctgatcag
     7801 tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt attgcgaagt
     7861 tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct gtgccataca
     7921 ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt tgcaacacag
     7981 gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa atatctgccg
     8041 ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg atcatcgtcc
     8101 ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa aaatctggcg
     8161 tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt agatccgata
     8221 cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt gcggccagcc
     8281 agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt tctgcatcag
     8341 acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc ttttcttccc
     8401 gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt gctgattgga
     8461 tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct tgagcttgtt
     8521 tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt gacgaacatg
     8581 cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg ctacatgacc
     8641 aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga tgacggctca
     8701 gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga agaagtcaag
     8761 aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga cccgatgatt
     8821 gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg tctgcatcaa
     8881 gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct ggacttaatg
     8941 ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc tttgcctcaa
     9001 aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca tttgactccg
     9061 ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac acttggaatc
     9121 attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa aatttttcgc
     9181 tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat tccatgcgct
     9241 tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct atgaataaat
     9301 ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta tctggctgtg
     9361 tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga gtactcgtcc
     9421 atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct tatggctggg
     9481 cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt attagtcaat
     9541 cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag
     9601 cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca caagcagaaa
     9661 tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc tgtctgcctc
     9721 tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat acagaaggta
     9781 tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta cttgttgcca
     9841 ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt cctgaagaac
     9901 agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga
     9961 tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc agctgtctgc
    10021 aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg gcagaagagt
    10081 tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca gaatcagcta
    10141 aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac agtacaaaca
    10201 ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat aagagtgtaa
    10261 taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt gaaaaagaaa
    10321 tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg atcgctatct
    10381 aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa cgtagacagg
    10441 tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc cttcttctaa
    10501 catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc ccttcgccca
    10561 tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga taccacccat
    10621 ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca tccctttttc
    10681 tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct tcttataaac
    10741 cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg ctcctttccg
    10801 caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa atttcttcaa
    10861 atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg gtatttaaaa
    10921 atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta aagacactct
    10981 tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac ttatcttatt
    11041 aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac cggacaacta
    11101 tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga caatgaaaaa
    11161 agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac tgctcttcga
    11221 accattctca gtgcaatccc taatttatct attaaatctt cttttagttc ctcatctttc
    11281 atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg cgaattgtaa
    11341 aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt tccgacgaaa
    11401 gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga cgaactgaca
    11461 atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa ccgaatggga
    11521 cgtgctatac aaactattta taatgtagtc aataagttca agcaaggcaa gactgctctt
    11581 gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt cattcaatta
    11641 cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac gcctgacgtc
    11701 attatcgggc gaaaagaaag gtctgtttca tgcggtatga gaacacttta tcgtttattt
    11761 tctaaaggaa tatttgatat tgacacacta ccgatgaaag gtaaaagaaa acccaatggc
    11821 catcaggaaa aacggggaaa acaacagtat cagcgctcaa tccatgatcg acctgataat
    11881 tatcctgatt tcaattctga gtttggtcac cttgaaggtg atacgatcgt tggcattcat
    11941 cataaaagtg ccgtcattac tttagttgaa agattatcta aagtcattat cacgattaaa
    12001 cccaacggcc gtaaggcatt agatattgaa actgccctta atcaatggtt ttctcgcttc
    12061 cctaaaaact tctttaaatc tattacgttt gactgtggaa aagaattttc taactggaaa
    12121 gccattagta accaacatga tattgatata tattttgcgg accctggaac accttctcaa
    12181 cgccc
//
LOCUS       DASJUF010000081        12168 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJUF010000081 DASJUF010000000
VERSION     DASJUF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.081"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..618)
                     /gene="frr"
                     /locus_tag="U4B62_002041"
     CDS             complement(61..618)
                     /gene="frr"
                     /locus_tag="U4B62_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN2142186.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(621..1343)
                     /locus_tag="U4B62_002042"
     CDS             complement(621..1343)
                     /locus_tag="U4B62_002042"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN2142187.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1479..2360)
                     /locus_tag="U4B62_002043"
     CDS             complement(1479..2360)
                     /locus_tag="U4B62_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN2142188.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2459..3241)
                     /gene="rpsB"
                     /locus_tag="U4B62_002044"
     CDS             complement(2459..3241)
                     /gene="rpsB"
                     /locus_tag="U4B62_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN2142189.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3600..4079
                     /locus_tag="U4B62_002045"
     CDS             3600..4079
                     /locus_tag="U4B62_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN2142190.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4296..5387
                     /gene="phnW"
                     /locus_tag="U4B62_002046"
     CDS             4296..5387
                     /gene="phnW"
                     /locus_tag="U4B62_002046"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN2142191.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5380..5508
                     /locus_tag="U4B62_002047"
     CDS             5380..5508
                     /locus_tag="U4B62_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN2142192.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5512..6057
                     /locus_tag="U4B62_002048"
     CDS             5512..6057
                     /locus_tag="U4B62_002048"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN2142193.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6511..8202)
                     /locus_tag="U4B62_002049"
     CDS             complement(6511..8202)
                     /locus_tag="U4B62_002049"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN2142194.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8621..9568)
                     /gene="arcC"
                     /locus_tag="U4B62_002050"
     CDS             complement(8621..9568)
                     /gene="arcC"
                     /locus_tag="U4B62_002050"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN2142195.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9683..10702)
                     /gene="argF"
                     /locus_tag="U4B62_002051"
     CDS             complement(9683..10702)
                     /gene="argF"
                     /locus_tag="U4B62_002051"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN2142196.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10793..12022)
                     /gene="arcA"
                     /locus_tag="U4B62_002052"
     CDS             complement(10793..12022)
                     /gene="arcA"
                     /locus_tag="U4B62_002052"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN2142197.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3330 a         2528 c         2203 g         4107 t
ORIGIN      
        1 agaaatataa ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta
       61 ttaaacttcc aataattctt tttctttatc agcagcgatc ttatctactt ctttgatgct
      121 gttatctgtc aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc
      181 accatttttt tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc
      241 gatttttgca ttttctgctt cttttttcac atctttagct aattcttttc tgcgttcttc
      301 tgtcagttgc gggataacta agcgaatgac atttccatca ttggttggac tgattccgat
      361 atcacttgcc ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat
      421 cattaaaaca cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc
      481 accgtagtag ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat
      541 ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt
      601 cataattgca tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag
      661 attgcacggc gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta
      721 tccatgctta gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc
      781 agatgagtca gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa
      841 acaccgtcta cattgttttt agccatcagg ataacatctg cgccgatttc tgccgcgcgt
      901 aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg
      961 atccgtcctt tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga
     1021 cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt
     1081 aaagctaagg cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc
     1141 atgcccattt gtgctccgat ttgtccgcgc cagatatttc cgccgcccac aacgattgcc
     1201 atttcaacgc ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt
     1261 ggtttgattc caaatcctgc gtccccagct aaagcttctc cactcaattt taagactaca
     1321 cgctgatatt tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca
     1381 tacttagatg atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag
     1441 cgcacaaacc gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata
     1501 acttcttcta caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg
     1561 aatgttttaa cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct
     1621 ttaacgaatg gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct
     1681 accatttttt caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca
     1741 gttttttcat gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg
     1801 atagctgcta cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga
     1861 acagtcaata cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg
     1921 tctttttcta caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct
     1981 tcgatcaagt cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg
     2041 tctgctggtt tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt
     2101 tcgtttttag aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct
     2161 ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt
     2221 gccattcctt tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact
     2281 aatgcttttt tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt
     2341 ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct
     2401 gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt
     2461 attctgctga agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg
     2521 tgttttcttc tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga
     2581 aagcatcagc catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag
     2641 ggattactac gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga
     2701 tatttaattt gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca
     2761 ttacatctgg gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac
     2821 gttctttgtt caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt
     2881 ccattgcatt gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca
     2941 atgttccgcc taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt
     3001 tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag
     3061 caacgctctt catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa
     3121 tgatgtagat tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac
     3181 gagtttggtg accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca
     3241 tgtttgtttc ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa
     3301 actttttcaa gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca
     3361 ccctcagcta ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc
     3421 aacattcaat agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt
     3481 tgtttcttta ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg
     3541 ggcaatcctt cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa
     3601 tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta
     3661 cgcaagaaga actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga
     3721 tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc
     3781 gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc
     3841 tggttcaaat gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg
     3901 tcaatacctt gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc
     3961 cctctattgt cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg
     4021 aggacgcaaa acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat
     4081 cagagcacgt attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttattg
     4141 aataaacctt tacactcttt acaaaaatac atcatttttt attcaaattt ttcaacacct
     4201 tgattgaatc acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct
     4261 ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta taaattatta acttctggac
     4321 cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc
     4381 aagaatacaa agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat
     4441 cggaaggaat atacacaacg gtcttaatgc aaggtagcag ctccttcggg gtagaatcgg
     4501 tattaagttc tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatata
     4561 gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat
     4621 cggaacatca aatcctccaa gctcatgtga ttacacagct gattgaagag aatccgtcta
     4681 tctccgcact agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag
     4741 agattgctgc tgtcacacaa gaaagagatc ttttgtttat agtggacgcg atgtcaagct
     4801 ttggtgggat accgatttgt gtagccgaac tagggattga ttttttgatt tccagctcaa
     4861 acaaatgtat tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactgg
     4921 aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct atatgaccag taccgcgtga
     4981 tggaaaacga gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc
     5041 aacaagcgct gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc
     5101 tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat ggggttccag cctttcattc
     5161 aagaatatca ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt
     5221 ttaatcattt ttatcagtat ttaaagcaaa atggatttgt gatctatccc gggaaaatca
     5281 gcgatcatga atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg
     5341 aactgacagc aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt
     5401 gattgggcag gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat
     5461 gcttttctag aaaaaggaac ccaactgact gacaaagaaa tccgctaacc gatgggaaaa
     5521 ttaaaatggg atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata
     5581 gctatttatg gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa
     5641 cattatctgt ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg
     5701 atcgaacggt tgagaaacgc tgggatcaat attggttcca cgaatggatt taatgctcct
     5761 atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt attcaccaga cttcatggca
     5821 acttttgaag acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg
     5881 cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat
     5941 atccaggaag ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta
     6001 atggggattt cttatgatga gtaccaaaaa ttagagatgg ggaaaaagat cgtttaaaac
     6061 agtctgttgc tcttagattc caacaagccg gcgctgatat ttgtgtaaac aatttaaaag
     6121 agttaacaga atacattttg tacgctgaca agaatccaga atgactttta tacaaaataa
     6181 gaggttgtga aaaagctgtc ctgtatcaag taataaaaac agccaagcaa aaatagctcc
     6241 tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat
     6301 tcgaaaaaaa aaagggagct gtgacacgac ttttgtcaca gtccccttat ttgcaaacaa
     6361 acagtgttta agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt
     6421 tgctcaaaaa acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga
     6481 tcagaacgct gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag
     6541 caaacgaagg tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc
     6601 tttttgttca tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc
     6661 taatttgatt gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac
     6721 cgtttcaggg tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc
     6781 atagtctgct gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc
     6841 atgtgtatat tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt
     6901 aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc
     6961 aacttgttta gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc
     7021 tttagcagat tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg
     7081 agttgataat tttttaccac cttgcgtgat caaaccaaat ggaatatggt gcatgtcgtc
     7141 agaccagtca aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg
     7201 ttcattccct actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc
     7261 agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg
     7321 attcaggtca taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg
     7381 tttttcttct aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt
     7441 caacgaatcg aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc
     7501 atcacggaac cattgccata attgaatagc ctctttatcg ccttcttcca gacgtttgaa
     7561 ccaagcacga gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac
     7621 gtataaacga agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt
     7681 tttgtaagcg acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg
     7741 gattggctga taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga
     7801 acgcaaatgt cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac
     7861 tgttccttga ttcccgatgt tgctatcccc ataatgttct ttttctttca ctactgtttg
     7921 caaaactttt ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac
     7981 ttcgattttt tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg
     8041 tggcgctttt cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc
     8101 agctgatttt ggattttcta gtaattgctc gacttgttcc agtgttaaat catctttgac
     8161 agcgtcatag acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt
     8221 gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac
     8281 tagagacgag atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct
     8341 gatatcggta gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct
     8401 tcttcattcg gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct
     8461 tgttcaacaa atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaacgaaag
     8521 gctagtcgtt tcataaaaaa aaaatccaga tggcctaaag gagaagaaat accacctgga
     8581 tagctataaa tcatttcgta aatacgaaag acttaacaaa ttaatcagct acgataatcg
     8641 ttccttcttc agaagctaaa agattctcga tattttctaa agaagtgatg attgccttag
     8701 cgtttggttt tgcttcaaca aatgcaatag ctgcttctac tttcggaagc atgcttcctg
     8761 gagcgaattg cttttcatca atgtattgtt tcatttctga aacagtaaca gtttctaatt
     8821 tcttttgatc tggtttttgg taattgacat acacattgtc tactcctgtc aatacgatca
     8881 gcaaatccgc atcgatgatt tcggctaatt tttctgaagc aaaatcttta tcgattacgg
     8941 cttctcgtcc ttctaatcca gtagctgttt ctacaacagg aatcccaccg ccaccaacag
     9001 agactgtgat aactccttga tcgaccagcg tttcgatgac acgtgcttct ttaatcgaga
     9061 tgggtttagg ggaagcgact acttttctcc atccacgtcc agcatcttct ttgaatgtca
     9121 cagtggagtc agcattcatt tgttctttgg cttcttcttc cgtgtagaat ggaccgactg
     9181 gtttacttgg atttttgaat gaaggatcat tttcatccac aataacttga gtgactaaag
     9241 aaaccacatc tttatcgatc cctttttcct tcagtacttc gcccatggcg ttttgtaacc
     9301 agtaaccgat agatccttct gtcattgcaa cacatgtatc taaaggcatt gccggtgttt
     9361 tctcagaatc agcagcttgc tgctgaatca ataggttacc gacttgcgga ccatttccgt
     9421 gggagatgat caattcatct ccttgttcga taaatttcac taaatacttt gctgtttcca
     9481 ttaacgcttc ttgttgggct tttgcacttg catccgtaga caagatggca ttgcccccta
     9541 aagcgacaac tacttttcgt ttggccattt cattcgtcct ttcttcagtt ctaccgaatt
     9601 ttctagataa caaaacgagc cagcgttttg tgattttccc tcaatgctga ctcgtttttt
     9661 cgtgagatga atgtggttat gcttaaacgc gagggataaa caagttacct aatgttgctg
     9721 ccatgatcgc tttgattgag tgcatacgat tttctgcttg gtcaaattga cgagcatatt
     9781 tgctgcggaa gacttcatct gttacttcca tttcagtgat gccaaagcgt tctttcattt
     9841 gttcaccgta aacagtgttt gtatcatgga aagctggcaa gcagtgcaag aagatcaaac
     9901 gatctgtgtt gtgtgttttt tctaccattt ccatattgat ttggtaaggt ttcaacaatt
     9961 tgatacgttc ttcgaattta tcttcttcac ccattgatac ccatacatca gaataaagga
    10021 cgtctgctcc gtctacacct ttatcaacat cgtcggtaat catcaattgt gcaccggatt
    10081 tttcagcaaa gccttctgcc attttgacga tttcttcttc aggttgtaat tcttttggtg
    10141 caacgatacg catattcgct cctaaaattg cacctgtgac taataaagag ttagccatat
    10201 tgtttcgacc atctccgcag taagcaacag tgataccttc tactgttcca aagttttctt
    10261 ggatagttaa aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc aagccattcc
    10321 aaactggaac accagagaat tctgctagtt cctctaccat tttttgactg aatccacgga
    10381 attcgattcc gtcaaacata cgtcctagta ctttagctgt atcttctgtg gattcttttt
    10441 tacctaattg gatatcattt gcacctagat attcaggatg tgcacctaga tcgatcgctg
    10501 cagtagtaaa tgcagaacga gtacgtgtag atgttttttc aaataaaagc gcaatatttt
    10561 ttccttctag atagtgatgt gggataccac gttttttcaa gtctttcaag tgttctgaaa
    10621 aatcgatcaa atattgtaat tcttcttttg taaagtcttt ttctgctaat aagcttcttc
    10681 cttggaatac tgattctttc atattaaaca ttctccttta gattgttttt atttaagatt
    10741 aaagaggaaa tcccgacggc agctgacagt aagactgccg tcaggaaaca aattacttca
    10801 agtcttcacg gacaagtggt tggctcatgc agcgtggacc accacgacct cgtgacaatt
    10861 cacttgaatt gatttctaac actttgatgc catagctacg aagcaattcg ttcgatacat
    10921 aatttcggtt ataagtgact acaacacctg gagcgattgc caatgtgttt gatccatcat
    10981 tccattgttc tcgaggtgca acgatctcat ctccattacc agttgggatc agtacaagat
    11041 catctaatcc aagagcagct ttcatcgttt cgtgtaagtt atctgaatga gtaatcttga
    11101 tatcatcacc atctggttcg attgtaaaga catccatctt accgtcttta ctttgaattg
    11161 ctggatggat agtgaattta tcgtgatcta ccattgtaaa tacggtatcc aaatgcatca
    11221 ttgcacggtt gttcggaatt ttgattgcca acactttttc aaatcctgaa ttttttgcga
    11281 ataggtggcg tgctaatttt tctaaagctt tcgcattagt tcgttgagaa atcccaacag
    11341 ctactacttt atcgcttaag atcaattcgt ctccgccttc gatatgatct ggattttcac
    11401 gatccaacca tacttcgatt cctttatttg caaatcttgg atgatgtttc aaaatatatt
    11461 ctgtaaacat cgattcacga cgacgtgctt caaaagtcat cttattgact gtcatcccat
    11521 ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg tccatgtaaa
    11581 atggatagtc atcgtctgct gacagatcat atagactgct agaacgtact tcgatatctt
    11641 ttgtacgaac cccagccatg attttatcta ccatttcttg cgtttccatt gatagaagga
    11701 actcatgaag accctctctt acagcattcg atgcaatatg agattcatcc aacattttat
    11761 tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat ttttccaagt
    11821 aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa gttgtcatgt tcttcttgag
    11881 caatcggaag atatggaata tcatcgaaca acagacgatc cataatatct ggtgtcaaat
    11941 tttccacttc ttgtccaggc cgtttcagta aaactgtctt taatttccct atttcagaga
    12001 aaacgtgaat aggtttatcc atgttgtgtt gcctccttct ttttgattac atgtatagga
    12061 tatcctcctt ttgtaatcac tttcataatt ttgtctcatt caaaatgaga cattcctcac
    12121 attaatattc gataattaaa taattaaacg tttttctatt tttaagcg
//
LOCUS       DASJUF010000082        12143 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJUF010000082 DASJUF010000000
VERSION     DASJUF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.082"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            248..1021
                     /locus_tag="U4B62_002053"
     CDS             248..1021
                     /locus_tag="U4B62_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2142198.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="U4B62_002054"
     CDS             1018..1785
                     /locus_tag="U4B62_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2142199.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="U4B62_002055"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="U4B62_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN2142200.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="U4B62_002056"
     CDS             3750..4070
                     /locus_tag="U4B62_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN2142201.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="U4B62_002057"
     CDS             4070..4420
                     /locus_tag="U4B62_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2142202.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="U4B62_002058"
     CDS             4587..5525
                     /locus_tag="U4B62_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN2142203.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="U4B62_002059"
     CDS             5540..6370
                     /locus_tag="U4B62_002059"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN2142204.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="U4B62_002060"
     CDS             6414..7439
                     /locus_tag="U4B62_002060"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2142205.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="U4B62_002061"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="U4B62_002061"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2142206.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="U4B62_002062"
     CDS             8517..8957
                     /locus_tag="U4B62_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN2142207.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="U4B62_002063"
     CDS             8960..9562
                     /locus_tag="U4B62_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2142208.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="U4B62_002064"
     CDS             complement(9625..10728)
                     /locus_tag="U4B62_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2142209.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="U4B62_002065"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="U4B62_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN2142210.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         4026 a         2103 c         2569 g         3445 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tat
//
LOCUS       DASJUF010000083        12018 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJUF010000083 DASJUF010000000
VERSION     DASJUF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.083"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(140..2215)
                     /locus_tag="U4B62_002066"
     CDS             complement(140..2215)
                     /locus_tag="U4B62_002066"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN2142211.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2217..3134)
                     /gene="glyQ"
                     /locus_tag="U4B62_002067"
     CDS             complement(2217..3134)
                     /gene="glyQ"
                     /locus_tag="U4B62_002067"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN2142212.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3520..4338)
                     /gene="recO"
                     /locus_tag="U4B62_002068"
     CDS             complement(3520..4338)
                     /gene="recO"
                     /locus_tag="U4B62_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN2142213.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4481..5380)
                     /gene="era"
                     /locus_tag="U4B62_002069"
     CDS             complement(4481..5380)
                     /gene="era"
                     /locus_tag="U4B62_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN2142214.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5395..5790)
                     /locus_tag="U4B62_002070"
     CDS             complement(5395..5790)
                     /locus_tag="U4B62_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN2142215.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="U4B62_002071"
     CDS             complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="U4B62_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN2142216.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6279..8471)
                     /locus_tag="U4B62_002072"
     CDS             complement(6279..8471)
                     /locus_tag="U4B62_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN2142217.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8495..9466)
                     /locus_tag="U4B62_002073"
     CDS             complement(8495..9466)
                     /locus_tag="U4B62_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN2142218.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10171..11550
                     /gene="brnQ"
                     /locus_tag="U4B62_002074"
     CDS             10171..11550
                     /gene="brnQ"
                     /locus_tag="U4B62_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2142219.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11832..>12018
                     /locus_tag="U4B62_002075"
     CDS             11832..>12018
                     /locus_tag="U4B62_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2142220.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         3285 a         2557 c         2196 g         3980 t
ORIGIN      
        1 tttcatgagc gttttgaaaa ttcgaacaaa gaataaaata acaaaaatta ttaaaataga
       61 attaataaca gctatttata acaaaaaaaa cagagacaca gcacgtttcg tgcatgctct
      121 ctgtttttca aacaaaagac tattttgtga tgatttgatc caagcttgct aatgacaaag
      181 ccatggcagc tatttgactt agttgtgtta gtcggttctt acgtactgct ggttcatcag
      241 ccatgaccat cgtttgatca aaataatttt cgatcagtgg acgaagagcg acaagctggt
      301 cgtagttttc gtggatcgtt tggtcagcaa aacttgcttt cattgcttct actgctttgt
      361 gtaattcttt ctctgcgtcg ttttcgaaca gctcagggtc tacttctgat tgtttttcaa
      421 gctgcgcttt tttcgctaag ttcacgacac gtgtcaaagc ttccattgaa ggtttgaagt
      481 cttcatctgc aagatgttgt ttgaacaatt gtgctgctgc aaatactttt gtcagatctt
      541 tttgttccgc attcaaaaca gcatcgatga catcatgacg aatattcttc gttgtcaata
      601 attggcgcag acggcctttg ataaagtcga tcacttgttg ttgcccttct gataaaccaa
      661 tgccgaaacg atccgtatct ttactgataa catcgctaat cgtttcttgc agaacagaca
      721 aaggaaaggc ccattttttc gattcgatga tccgaacgac accgtatgct tggcgacgta
      781 aggcataagg atcgtttgac ccgcttggaa tcatcccaac tgcaaagaaa gaaaagatgc
      841 tatctaattt atctgcaatc gctaaaacag aacctactgt agactgaggt agctctcctt
      901 cactggatat aggcatgtaa tgctcacgaa tcgctgtcgc tacagcaggt ttctcgcctt
      961 tcagaagagc atatttttcg cccatgatcc cttgtagttc aggaaattcc cctaccatgt
     1021 tggttactag atcaaattta tagatttcag cagctcgttg caaatccatc aattcttcct
     1081 cagatagtcc tacttgttcg ccaatgatac gggcaatgac ttgtacccgt tgcattttct
     1141 catagatact accgattttt tcatggaaag taacagtttt tagtttttca acgtattggt
     1201 cgattgaagc tttcttgtct tcattataaa agaattccgc atcttctaaa cgagccgtca
     1261 atactttttg attgcctcga accacatctt ccaattttac ccgatcacca ttacgagcag
     1321 aaataaaatg aggaagcagc ataccttgat cattccgtac ctcgaaataa cgttgatgtt
     1381 ctttcataga agtgactagt acttcttcag gaacagaaag atatttttct tcgaagtcgc
     1441 cagaaaagac agttggatat tcgactaaat tgttgacttc ttctagtaaa tcttcgtcta
     1501 gatcaataac ccagtgattt ttttctgcta attcgtttgc ttgtgtaacg atcatttctt
     1561 gtcttttttg cggagacgca atgacgaatt gttcattcaa tttttcttca tattcttttg
     1621 catgcgcaaa tgtcacatct tcgcctaaga aacgatgacc tcgagaggct ctgcctgttt
     1681 tgacatccaa gatttcgaat gggattactt cgtcatctaa caatgccacg atccagtgaa
     1741 tagggcggat atattcaaaa tcgtattctg cccaatgcat cgttacaggg aaagtcaaac
     1801 ttttgatcac ccctgctaag cctgtcaata cttcttttgc ttccttacca tgagtgtatt
     1861 tcgttacata gacatattcc acacctttta attctctaaa agtgatagcg tctgtcgtca
     1921 gaccttgtcc gcggacaaat ccttccgctg ctttcgtcca gtttccatct gcgtccaaag
     1981 caattttttt ggctggtcct ttgacttctt cttcactgtc cgcttgtcgt tctggaatat
     2041 ttgttacttt gattgctaaa cggcgcggtg tggaaaatgt ttcgatcgta tcataagtca
     2101 attggttttc atctaaaaat tttgctgttt tttcttctaa ttgtttcatg cttggtgtga
     2161 caacatgggc aggcatttct tccagcccga tttcaagtaa taagtctttt gccatcttat
     2221 tcagcctcct tcacggtttc tggatttgct gtgtttttat tcaacaaagg gaatcctaat
     2281 ttttcgcgtt ctgccacaaa gattttggct acagcacgag ccatgttgcg gatgcgagaa
     2341 agataaccgg cacgctctgt tacggatact gcaccgcggg cgtctaataa gttgaatgta
     2401 tggctacatt tcaagatata atcatacgcc ggatgaacaa gattttcatc gatacagcgt
     2461 ttagcttctt tttcaaaacg atcaaagttg cttagtagca agtcttggtc gcttacttca
     2521 aaagaatatt tggaatgttc atattctggt tgtttgaaga tctcgccata tttcacgcca
     2581 tcagcccatt ctaaatcata gacactttca acttcttgga tataagaagc aagacgttct
     2641 aatccataag tgatctcaga agttactggt ttacaaggca gtccaccaac ttgctggaag
     2701 tacgtgaatt gtgtgatttc cattccgtca agccatactt cccaacccac accggcacag
     2761 cccatggatg ggttctccca gttgtcttca acaaaacgga tgtcgtgttc taatggatcg
     2821 attcctaaaa ggcgcaagct ttctaaataa agctcttgaa tattctcagg tgaaggtttc
     2881 atcaccactt ggaattgatg atgctgatac aaacggtttg ggttttcacc ataacgtcca
     2941 tcagctggtc ggcgagatgg ctctacgtaa gcagcattcc atggttctgg tccgattgcc
     3001 cgtaaaaagg tatatgggct catcgtacct gctccttttt ctgtgtcata agcttgcatc
     3061 agcatgcagc cgtttgatga ccaaaatttt tgtaatgtta aaatcatatc ttggacggtc
     3121 aattttttac tcatctgttt tgcccctttc aattttaaag cgttgctgtc aaataagaat
     3181 acaggaaaga aaacgacaaa aaatccctat gtctgattac agacataggg acgaataatt
     3241 cgcggttcca ccctacttcc gaataaagat tcggcagctt catttatcgt actcgcaagt
     3301 gcctcttcaa aaatcgtttg ggtccggctc ccactctccc gaactcgcta aaccacgtgt
     3361 tttttactga tcttggtcaa tgtacgtatt cattttgcag ttttcttgtt tctataatag
     3421 atcctatcac tgaacgatct atttaatcta tggttaataa tagacggaac tccctgtgtt
     3481 tgtcaaatgg ttttgatttt ttgttgtatg ttttatttac tattcttctt tttcaggtgt
     3541 ctctttttct ttttcagaca tgtctttctc tgacgtcttt aatacgtgtg cccaagactt
     3601 catttgattg atgaactttt tgcttttcaa atggataccg acatattcat catacagctg
     3661 gtcgatgagt tcacggattg cttgtttggt ttcttctttc agatggattc cactaatttt
     3721 atcataagaa atagctgaaa acatccgaac gaaataaaca gctcgaggat ctgcatgata
     3781 acggtgatcg tccatcgacc aatgtctttc gcatatcaca ccaccatatt tggaagaata
     3841 gtcaaatttt ccttttgttt ccccgcatac gacgcagtgt gtccagattg gtgcaattcc
     3901 gaagcgttgc agtatttgga cttcaaaaat attcgtgatg atttctgcat cgtctccttg
     3961 gtccatccgt tgcagtgctt gttgcgtaaa ctggaacaaa tgcgggtcgt actgatgatc
     4021 ttcgattgcc gcatctacta gattcaataa atatgtgcca tagccgctca aaaagatgtc
     4081 ttcttggatg tgtcgaaacg catggacatc tttgacactg cttaaaaatg acaaaccttc
     4141 acttcgaaaa ttcccgatat aaactgcttc agtaaaaggg agcaaagccg gagctaaggg
     4201 ctgattttgc cgatggatct ctttgatata aaacatcatt ttgccagaag attctgtaaa
     4261 gattttgact aatttatctt tttctttgta attacggcta gataaaatca agccttttga
     4321 ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag cagattctgc
     4381 cgatccagat catttatgtt gcgatcttcc gcagcttttc tcaacacgcg gatttttatt
     4441 cagtttattt ctatacctaa tttacaagaa gctcagaata tcagtattcg tcttttcggt
     4501 agccgaagtc ttgcagatag acttttttgt ctcgccagtc tttttgaact ttgacccaaa
     4561 gttctagaaa gactttgtct cctaaaagat tttcgatatc ttttcttgct ttcgttccta
     4621 cttgcttcag catttttccg cctttgccga taatgatccc tttttggcta tctctttcga
     4681 caataatcgt agcctggata tggattttat cattctcgtt tcgcttcatc gaatcaacaa
     4741 cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc tcacggatca
     4801 attcagaaac gatgaagtat tcaggatgat cagtcacttg atcatctgga aaatattgtg
     4861 ggccctctgg catttcactg actaatacat ccatcaaacg ttcgacgttg tttccttctg
     4921 tagcagagat tggaatcact tgtgcaaaag gcatctgcgt tgaataatct tcgatgattc
     4981 ccaacaactc gtctggatgg attttgtcga ttttattgat aatcagataa acaggagaat
     5041 gcacgttttt tagacgttca ataataaaat cgtctccccg accgcgtttt tgatcagcac
     5101 taatcataaa taagaccgcg tccacttctt tcaacgcact gtaagctgct tctaccataa
     5161 aatctcctaa acgatgcttt ggtttatgga ttcctggtgt atcaataaaa atcaactgag
     5221 cttctggtgt agtgtatact ccttggattt tatttctagt tgtctgtgcc ttgtcactca
     5281 tgatggcaat tttttgaccc acgattcgat tcaataatgt ggatttccct acatttgggc
     5341 ggccgacgat tgcgacaaac cctgatttat gttctggcat atatgttctc ctttttaatt
     5401 aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaacgatga cggtaaaaca
     5461 agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa tcggatgaaa
     5521 atgaaaatcc gtaaacatgt ccacgacatt ttcaaaaaca gtattgatga tctctactat
     5581 ccatacgaga aaaacagcta aaagcagcca taaccattct atccgatcaa gttggaaaat
     5641 cacgcccata aacaaagcga ggattccgaa cgcaacatgt ttcctcatgt tgcgttcttc
     5701 ctcaaaaaca gttttgactc cttggacggc aaattccacg gacatcagga aatgtttgtt
     5761 tttctctacg tttttatctt ttaagtccat aggcatctaa gatctctttc tgtaaaccaa
     5821 acatttcttt ctcatcctct ggtgtcatat ggtcataccc gttgatatgc aagaatccat
     5881 gaaccgctaa aaagccaagt tcccgatcat acgaatggcc gtaagcttct gcttgctctg
     5941 cagcacgttc agtagagatc atgatgtctc ccagtacacg aggcaatgga ttctctccat
     6001 cttcaaaaat cactgggatt tcgtcttcgc cctcttcttc caaggcaaaa ctgatgacgt
     6061 ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggattgct gcattgtcca
     6121 tgaaagtaac agacatttct gtttcttctg gcaactctaa atatgatgct gcaaattgca
     6181 gcaagtcgtc gatttctttc attttctctt ctgagacttg gtttgtttca tccatgaagg
     6241 taatatccat tatacatcgc ttctttctat tcttcttgct attcttcttg ttcttctttt
     6301 atcttctcaa cttccgattt cattttagga tatttgatcc gagaatggaa ggtactgctt
     6361 aaaccgctga tcaatgattt ttctacttta cggatttctt ttaacgtcaa tccactgtcg
     6421 tccaattgtc catcggaaat acgctcttca ataagattat ggacaaaact ttcgattttt
     6481 tcgctcgttg gatggtccat tgcccgtact gccgcttcac aactgtcagc tatattgacg
     6541 attcctgctt cacggctttg aggttttggt ccaggataac ggaattgctc ttccgtaact
     6601 tccgggttgc gttcctttgc cttaacgtag aaataacgca tcagcgtggt tccatgatgc
     6661 tgtctacaaa tatcgatcac catttgcggc atattataat cttccaatat tttggcacca
     6721 tctgtcacat gtccaaagat aatatgttta ctgtcttctg gcaataagaa attgtgcgga
     6781 ttttccgctc ctgaaggcaa gttctcaacg aaaaagctgg cgtgtttgat cttgccgata
     6841 tcatgataat agcaagctac tcgagttagt aaagaacgtc caccgatttc tgctacagca
     6901 ttagcactta gattggctac catcatgcta tggtgatacg tacctggtgc ttcttccagc
     6961 aactgtttca ataaaggatg attgggattg ctgagttcat ttaatacgat cacactatca
     7021 tcattgacca tcaattcaat ataggggtgc agacctatcg tcagcaagaa tgaaaacagt
     7081 gcacctgtga atccgcagac tagcgttaac caagtcgttg catccccaaa gctcattcct
     7141 tggtagataa tcaaaatgac atccatcatc acaggaaaaa taatgaccca catagcagcg
     7201 gataaccctt gttctgagat ccgtttgcgt ctgacgatcg ttgccatgag accagaaaat
     7261 acataggaca tcaaaataac tgtcagaaag ttcgtgccaa tcgaatcata aaaaatgaaa
     7321 agtgcggata ctgcttgaaa aataccagcc atgatccctg ctcttcgatt caagaaaaag
     7381 cttaaaacaa gtggagcaaa agccgcgggg taaaacagcg gaatataagc agcttgttct
     7441 gtttggaaga gttggaaaaa tttcattagt acgacgctta ctgacattgc cgtcacgtag
     7501 aaaagtacgt attttgttct ttctcctgca ttttggaatt gaagtgaatt ataaatcagt
     7561 acaccgattt gaagcaaaac agctaaaacc atggcaatga gtgggaaaat agaagtcgtt
     7621 tgattcgtca atcctagtaa ttccaatttt ttcatcgctg aagcatcaat ttgatttcct
     7681 tcgcgaacaa taatttctcc ttggaagatc atcactggtt gtacagcttc tcgtgcagat
     7741 tgttttaatt cttctgtctt tttctcattc aatacatcat taactacgat gccttgatcc
     7801 actaagtagc gaatcatttg ttgctgctct gttgtgacat tcaaatattg aatctgatct
     7861 tctgcttctt gtttaaaagc ttccaattcg ctttcacgaa cttgtttact catctgttcg
     7921 tcaatgagct tcaaactttc atccctaact gtgtgaattt ccgtttcagt catttcaaat
     7981 atcgtcgaat aaaaattgtt cggcagtttt tggtaaaacg caacgttttc tgcattcacg
     8041 ttttcaaaat ttttcttcag tgtagcaatc cgctcttctg tagtaggttt cgggatattt
     8101 tcatcttcct tcgccttatc taccttgcca ttataagatt tgttgatggt atcattcgtc
     8161 ttatcgatca attcgaataa ttgcttgatc cgattgttct ggtcatctgc cagatctttt
     8221 tgataagtat attctggagt cactgcttct gctgccagtt ttcttctttg ttcggtttct
     8281 tccgtgttct caatcgtttt attcgcacgg atactctctt ctgctacttg gccttctctg
     8341 aaatcaatgg ccttttgttt gacactacta aatgtaacgc cgcagacaat gataaaaaac
     8401 aagaacagaa ggaaaggcaa tgctgccttt ccgagttttt cttgtagttt ctgcagtgtt
     8461 ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatgaaga tccgcctttt
     8521 cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt gcttcaaaat
     8581 taacaaaatc gatttttttg atttgcttta atgttcgttc tgcatgaaca agaccacttg
     8641 tcgtcccacg aggcaagtct atctgacttg tatccccatt gacaatcatt tttgattgat
     8701 aaccgagacg ggtcaagaac attttcatct gagcgactgt cgtgttttgt gcttcgtcca
     8761 agataacgaa tgcatcatcc aatgtccgcc cccgcatgta agctaaaggt gcgatttcga
     8821 tcacgcctcg ttccatcaat cggttcgtat gatccaatcc aaaaatttgg tataaagcat
     8881 cataaacagg ccgcagataa ggatccactt tttctttcag atccccgggt agaaagccta
     8941 ggttttcccc tgcctccaca gcgggccgcg tcaaaatgat tttttgcact tcgccttttt
     9001 ttagggcagc aattgctaat actacggcaa gaaacgtttt ccctgttccg gctggcccta
     9061 caccaaatac gatgtcatgt ttggctaccg attcaacgta tttcttttga ccgctgtttt
     9121 ttacccgtat aggttttcca ttacggtctt tgacgatctc ttcctcatac atctcaacaa
     9181 aataatctaa tgtacctttt tgccccattt tcaatgccgt cacaacatct ggcgtactta
     9241 cgtggattcc tcgtttgatc aattcctgta atgtccgaat gactgaggca gccaaggaaa
     9301 tctgggattg ctcaccaatg atctgaatga tttctccacg tgtatggatg gttgtttccg
     9361 ttgtttcctc gatcagtttt atatgcttgt cgtgtgcacc taaaagcatg ctgacatcat
     9421 cagcagctgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat agacatcccc
     9481 tttttttcga ttcattcttt cctattttac catactcaga agaaactata tagcaaataa
     9541 gaaaaggttg taaaagagtc gcttaaccat acgtaccaag aaacgttaaa aaatagccgt
     9601 ttccattcgc taaaggtctc gttttgataa aaagaagctg agaatgtaag ttatatgaaa
     9661 gtggagaaca aaatatttcg tttcattcaa caagtattct ctaggtattt ttcctgtttc
     9721 aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca ggtgtattat
     9781 tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa cagtaggcta ttcaacgatt
     9841 tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg aacatcattt
     9901 ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg ctagcggata
     9961 actttatcaa aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg
    10021 ttctagaatc tagagccctt ttcccaagtg gaaaagggct tttttttatt ttaaggtggt
    10081 ggttttcatg gaatctgata ttagtggcat tactaattgc tgcaacgttt atatcgtcaa
    10141 tcaaaatgat aattattgga ggaaaaattt atggaagaaa caaaaactta ttcatggaaa
    10201 caactattga tggtcagttc acttatcttc gggatgttct tcgggtcagg taatctaatc
    10261 tttcctgtcc acttaggtca aatggctggc gcaaactggg tcagtgctgg cgtaggattt
    10321 gctatctcag gtgctctttt cccattacta gctatcttag ctgtagtagt gacaaaaagt
    10381 gatggtctgt atgacttagc taaacctgtc ggaaaatggt atgctgcctt gttcttaatc
    10441 cttgttcatt tgacgatcgg accatttttc ggtacaccac ggacagctgc tactgctttt
    10501 gaaatggctg ctaaaccatt tttgcctgaa aaatataatt cattaggtat gtttttattc
    10561 tctgctcttt tctttttact tgcttatttc cttactgtcc atccaacacg gttggtcaaa
    10621 tacgtcggaa agtatctaaa ctccttgttt cttgcattat tagcaatcat ttttgtcgtt
    10681 tctttattcc atccaatggg aaatctaaat caatctgctg cagatactta tcaaacaaac
    10741 gctttaacaa atggtgtcct agaaggttat aacacggttg atgcagtagc tttgcttgcc
    10801 ttaagtgtca cttttgtcca tgcagtccgt gaccttggtt tcaaagataa aaaagtcaca
    10861 gaattaacgg caaaagctgg tagcctgagc gttgcgctag aagtcatgat ctattttgga
    10921 cttgttcttc tcggtgcgat gagtttgaat aagatgtctt tatcagaaaa tggcggaact
    10981 gcactttctc agatcactaa tttttattta ggtaacttcg gcgctagctt cttaggagtg
    11041 atggtcacat taggtgtctt cactaccgct atgggactcg ttgtttcttt tgcgcaagat
    11101 ttccataaac tgttccctaa agtcagttat atgacatggc tgcggttaac gactttcgtc
    11161 tcttttgtcg ttgcaaatgc tggtttagac aatatcatcc aatggtcttt accagtattg
    11221 atgcttttgt atccattgtc gcttgcgttg atcttgcttt ccttaacagc aaaatttttc
    11281 caaaagacac catttgtcta tcaagtaacg atgttgtttg ctgctgttcc tgccgtattg
    11341 gatatgctgg caaattcgcc tgctttagtg agtcaacaaa gagttgtagc aagcatgctc
    11401 gaattttatc atcatcacgt tccttttgct gcattaggac ttggctggat ggtcccaacg
    11461 ttacttggtt acgctggcag cctactgttt tattacgcct atcgcctatc agggtataaa
    11521 caagaagcaa atgaactacc agaagaataa ccaaatcaaa cacaatttaa cataagaaat
    11581 attaaacagg aaaaaaccag ctagatgaaa gaatcctatt ctttcgtcta gctatttttt
    11641 agttctcttt tttacgcgca caaaaaaact gcggacaatg attgtccgca gtttaaatgg
    11701 gctctataat atctagtgtt ggtgacgcct tctgttgcca atactttttc ttttttctaa
    11761 taaacgcaaa cagacacctt gaaatccttt agaataaagt cgacgaaaac cattcaaagg
    11821 agtgtttcaa gatgtcctat tcagagttta ccaaagagtt actagatatt ctagacctaa
    11881 atcttatctt tcatgaagat gcctttcgca aagagcgaat caatgacgaa acatgttttg
    11941 tttttgatgg cacattgacc tatcaaccag aggaatgctt ccattgtcac tatcaaaata
    12001 agcaaacgat cattaaat
//
LOCUS       DASJUF010000084        11972 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJUF010000084 DASJUF010000000
VERSION     DASJUF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.084"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            194..1009
                     /gene="thiD"
                     /locus_tag="U4B62_002076"
     CDS             194..1009
                     /gene="thiD"
                     /locus_tag="U4B62_002076"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN2142221.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1338..2789)
                     /gene="cls"
                     /locus_tag="U4B62_002077"
     CDS             complement(1338..2789)
                     /gene="cls"
                     /locus_tag="U4B62_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2142222.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3130..5796
                     /locus_tag="U4B62_002078"
     CDS             3130..5796
                     /locus_tag="U4B62_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2142223.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6160..6912
                     /locus_tag="U4B62_002079"
     CDS             6160..6912
                     /locus_tag="U4B62_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2142224.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6934..8088)
                     /locus_tag="U4B62_002080"
     CDS             complement(6934..8088)
                     /locus_tag="U4B62_002080"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN2142225.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8273..10714
                     /locus_tag="U4B62_002081"
     CDS             8273..10714
                     /locus_tag="U4B62_002081"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN2142226.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(10871..>11972)
                     /locus_tag="U4B62_002082"
     CDS             complement(10871..>11972)
                     /locus_tag="U4B62_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HEN2142227.1"
                     /translation="GSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         3847 a         2147 c         2569 g         3409 t
ORIGIN      
        1 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
       61 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
      121 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
      181 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
      241 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
      301 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
      361 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
      421 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
      481 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
      541 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
      601 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
      661 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
      721 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
      781 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
      841 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
      901 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
      961 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
     1021 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
     1081 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
     1141 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
     1201 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
     1261 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
     1321 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
     1381 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
     1441 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
     1501 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
     1561 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
     1621 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
     1681 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
     1741 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
     1801 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
     1861 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
     1921 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
     1981 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
     2041 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
     2101 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
     2161 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
     2221 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
     2281 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
     2341 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
     2401 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
     2461 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
     2521 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
     2581 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
     2641 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
     2701 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
     2761 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
     2821 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
     2881 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
     2941 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
     3001 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
     3061 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
     3121 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
     3181 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
     3241 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
     3301 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
     3361 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
     3421 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
     3481 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
     3541 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
     3601 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
     3661 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
     3721 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
     3781 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
     3841 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
     3901 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
     3961 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
     4021 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
     4081 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
     4141 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
     4201 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
     4261 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
     4321 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
     4381 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
     4441 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
     4501 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
     4561 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
     4621 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
     4681 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
     4741 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
     4801 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
     4861 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
     4921 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
     4981 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
     5041 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
     5101 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
     5161 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
     5221 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
     5281 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
     5341 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
     5401 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
     5461 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
     5521 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
     5581 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
     5641 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
     5701 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
     5761 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
     5821 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
     5881 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
     5941 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
     6001 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
     6061 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
     6121 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
     6181 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
     6241 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
     6301 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
     6361 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
     6421 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
     6481 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
     6541 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
     6601 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
     6661 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
     6721 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
     6781 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
     6841 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
     6901 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
     6961 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
     7021 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
     7081 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
     7141 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
     7201 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
     7261 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
     7321 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
     7381 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
     7441 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
     7501 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
     7561 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
     7621 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
     7681 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
     7741 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
     7801 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
     7861 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
     7921 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
     7981 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
     8041 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
     8101 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
     8161 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
     8221 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
     8281 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
     8341 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
     8401 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
     8461 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
     8521 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
     8581 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
     8641 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
     8701 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
     8761 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
     8821 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
     8881 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
     8941 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
     9001 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
     9061 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
     9121 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
     9181 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
     9241 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
     9301 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
     9361 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
     9421 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
     9481 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
     9541 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
     9601 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
     9661 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
     9721 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
     9781 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
     9841 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
     9901 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
     9961 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    10021 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    10081 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    10141 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    10201 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    10261 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    10321 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    10381 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    10441 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    10501 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    10561 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    10621 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    10681 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    10741 gcaagattta cagttatttt atttactgca gatcttgctt ttttggctct tcgtcaaatt
    10801 gtgttggcga agacaaattt gaataaaact tagtccgata tgcggcgcgt tctgtgcgcc
    10861 gcatattttt ttagttttca aagatcagac cttgttgtaa atagatccgt gcccgaaagt
    10921 tataaaagtt gcgataacca aacgcaattc gtttaatcag cttgatcttg ttattcagcc
    10981 cctcagtgac gccgtttgag tggggcacct taaaggcatt tgaaataccc gattggtagc
    11041 gatggaagat cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa attgttttaa
    11101 gggtttcttg aaagccttcc gtatctcgtt taacgaaata atagcgtaat aattgacaaa
    11161 aatgatacgc agttcgcaat tcttcgttgt agcttaacag ctcatcaacg atttctgttt
    11221 gactaattgg tcgtttaaac agtcgatgat aatgccgttt gagtggctcc aatgtcgtgg
    11281 aatctttgag taagagtttc caatatcgct tgagccgacg gtacttcttc tgattttcag
    11341 agtgtgaggt tcgaaaacga ttcatgatct ggacacgtaa ttgattgaag ctgcgattga
    11401 tgtgttggac aatatgaaag cggtcagtta cgatctccgc atttggaaag actgttttaa
    11461 gcaactgaca atagctggca ttcatgtcca tcactaggta cttgaccttt aaacgggcct
    11521 ttctaggaaa gcgcaggaag tattgagtta gtttgtaaag tctgcgatct tcaaggatcg
    11581 taaatagctg attggttgtc ccatcaacac aaataaaact catggaacca gaaacagact
    11641 tcattgattt gaactcatcc atacacaaca ccgtcggaag gaagttcata cgtggctggt
    11701 aatgcttcgt tgcttcacgc aagatccgtg tcaccgtccc atccgagacg aagtacttct
    11761 tagcgattag tttacgtgat tgattttcgt acagttccat gagaatcgcc tgcttcagtt
    11821 cctttgttag ttgatgatgt tcatcaacta agtccgtttt cgcagaaaag gtccgtccac
    11881 aatcatggca gagaaaacgg gtacgtttta attctaaaat agtcaacttt tctttgactt
    11941 tcaacatctg agtgcgtgtc gtataggaac cg
//
LOCUS       DASJUF010000085        11457 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJUF010000085 DASJUF010000000
VERSION     DASJUF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.085"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(152..637)
                     /locus_tag="U4B62_002083"
     CDS             complement(152..637)
                     /locus_tag="U4B62_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN2142228.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    727..1072
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1117..1758
                     /locus_tag="U4B62_002084"
     CDS             1117..1758
                     /locus_tag="U4B62_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2142229.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1777..2412
                     /locus_tag="U4B62_002085"
     CDS             1777..2412
                     /locus_tag="U4B62_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2142230.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2430..3245
                     /locus_tag="U4B62_002086"
     CDS             2430..3245
                     /locus_tag="U4B62_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2142231.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3564..5000
                     /locus_tag="U4B62_002087"
     CDS             3564..5000
                     /locus_tag="U4B62_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2142232.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5189..7636
                     /locus_tag="U4B62_002088"
     CDS             5189..7636
                     /locus_tag="U4B62_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN2142233.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7866..8933
                     /gene="rlmN"
                     /locus_tag="U4B62_002089"
     CDS             7866..8933
                     /gene="rlmN"
                     /locus_tag="U4B62_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN2142234.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9126..10229)
                     /locus_tag="U4B62_002090"
     CDS             complement(9126..10229)
                     /locus_tag="U4B62_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2142235.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10782..11330
                     /gene="aac(6')"
                     /locus_tag="U4B62_002091"
     CDS             10782..11330
                     /gene="aac(6')"
                     /locus_tag="U4B62_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2142236.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3801 a         1941 c         2474 g         3241 t
ORIGIN      
        1 atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact agttgttaca
       61 ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga aaaagacgct
      121 tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact ggcagatgtt
      181 ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta gtggtattga
      241 tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc acgacttgga
      301 tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct ggaacgccta
      361 ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt aattgttcag
      421 ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag atttgttttc
      481 cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa aaagggacat
      541 ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc agttttttta
      601 gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct ccttttttta
      661 ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt acacgtaaag
      721 ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag agtctctggt
      781 tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt tctgaagcgt
      841 atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg accagataag
      901 gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta acgtcctttg
      961 atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg tgaggttttt
     1021 tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct tggcatttgc
     1081 caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat tgaagttatg
     1141 ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac cttgattggc
     1201 tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa gccgctacgc
     1261 tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt gcaactgatt
     1321 tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga agcagcactc
     1381 attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg agggatacaa
     1441 tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc ctttcaaacg
     1501 gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt agggaatgaa
     1561 gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg tgacgtaatg
     1621 cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct aggcgtggca
     1681 atcatctatc ttacattgac tggtattcta acaatcataa tgaaacaagt tgaaggaaaa
     1741 ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa gaaagtgaac
     1801 aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc agatgggaaa
     1861 acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg tatcttggct
     1921 ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt caacccattc
     1981 gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca gctatttcca
     2041 catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa acagacaaaa
     2101 gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag tgatttattg
     2161 aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagctat tgccagagca
     2221 ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt agatcctgaa
     2281 ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt aacccaaatt
     2341 attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact aaaagtttcc
     2401 ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta ttcgctttga
     2461 tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca gataattggg
     2521 cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt gttcctatgg
     2581 gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc aaagcagtct
     2641 ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg aaggaatttg
     2701 aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg ccggcaagag
     2761 aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg atcacaccta
     2821 aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt gcgcaaaatg
     2881 ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag atagtcaaaa
     2941 accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg aaaagcagac
     3001 gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa caaagaaacg
     3061 aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt gcagttggtg
     3121 cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat caactagaga
     3181 aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt acgccgagaa
     3241 attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc gaataaacag
     3301 tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa cctcttggta
     3361 aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa atgtagaaag
     3421 ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac aacctccttt
     3481 acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg aacgattgaa
     3541 ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt ccaaaagatt
     3601 ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa gaagatggga
     3661 aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt aaaggaacga
     3721 acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct ttaatgaaag
     3781 aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt cctaatggac
     3841 gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac gaattgattg
     3901 aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa gcattgcaag
     3961 atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat tatgcagaaa
     4021 cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat gaacaaaata
     4081 tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca gacccgaaac
     4141 gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc aataaatttc
     4201 acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca aattatccaa
     4261 ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg atggccaatt
     4321 attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg ttcttagaag
     4381 aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa tctgctaaac
     4441 cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat ccattaaacg
     4501 gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa gaaactggag
     4561 ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat tgggactggg
     4621 agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt taccgcatgc
     4681 cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa gataaacaaa
     4741 tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc aaagaagcaa
     4801 tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat ttgcttagct
     4861 ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac gaaacacaag
     4921 aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa ctgattgaag
     4981 aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct ggatatggcc
     5041 gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata agctgaaaaa
     5101 tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt ttttgtataa
     5161 tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta ctaaaaaaag
     5221 gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc tgacgattgg
     5281 tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag gttttttagg
     5341 aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa tcctctgtct
     5401 tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt caattggtaa
     5461 aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat tgttacatgc
     5521 ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga catgggattt
     5581 gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg ggatgattgg
     5641 agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt atttagcagc
     5701 agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct atcagctgtt
     5761 aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg atcctgccaa
     5821 gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag aagcgaaaga
     5881 agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat atgagaagaa
     5941 aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac cagaaaatac
     6001 agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata tccatcctgc
     6061 gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag aaggtttttc
     6121 agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag cagaacagga
     6181 aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa ctgtggatca
     6241 aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag
     6301 tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca caaaattcga
     6361 agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag atgatatcgc
     6421 gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta aatcactgat
     6481 tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa tcatcgaagc
     6541 acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg tttctggacg
     6601 ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt cgactggtag
     6661 tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg ctaagcccca
     6721 tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt acaacggcat
     6781 acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa
     6841 agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag taagaaatat
     6901 ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga aacatccaac
     6961 cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg tagccagcaa
     7021 tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg ggattcatat
     7081 gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca aagcaaatgt
     7141 accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga tcattgattc
     7201 taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa tgggtgagaa
     7261 taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac gggtcgttca
     7321 atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga cagatgaagt
     7381 tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag caaaagcact
     7441 tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc ggatcggtta
     7501 caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg gcccgtcaga
     7561 aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa cgttgccgga
     7621 cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac caataaaaag
     7681 acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct aatacggtat
     7741 gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat ttgtgaagta
     7801 ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa
     7861 atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc aattggttta
     7921 tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg tatcaaaaac
     7981 gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa aaattatcgg
     8041 agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg gatggtacag
     8101 taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg atgagacaag
     8161 aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc tgtacgtttt
     8221 gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt gttgcacaga
     8281 tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg
     8341 ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac tttttgcata
     8401 tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt tccactagtg
     8461 gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat ctggctattt
     8521 ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc
     8581 caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac cgtcgaatca
     8641 cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg caacaattag
     8701 cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca tataatccag
     8761 tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga ttttatgatg
     8821 tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca gatattgatg
     8881 cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc
     8941 ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga actgttcaac
     9001 ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac aagaaaaacc
     9061 ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg ggttttagca
     9121 attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg ataagtttct
     9181 acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac atgtttaata
     9241 tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac attgagattc
     9301 atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat tttatcaaac
     9361 aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac agatgctccg
     9421 tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga gtgtgccata
     9481 gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac aatgatatcc
     9541 gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt caatgcttgc
     9601 gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc taatctatcc
     9661 gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt ggataaagca
     9721 tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc tgtatcaatg
     9781 aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta
     9841 tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg aaaggtgaag
     9901 actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg gccaccaccg
     9961 actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg ttcaatattt
    10021 ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa
    10081 gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat cagcactact
    10141 tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt tccaatggta
    10201 taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg ttgaaacggg
    10261 ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga ggtgaaccta
    10321 agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa taagtatcct
    10381 aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt tcctcttagt
    10441 atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa attaagatta
    10501 ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa gcaaaagata
    10561 atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt cctgatcttt
    10621 cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag ataaataatg
    10681 aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc
    10741 aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg
    10801 accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg
    10861 aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg
    10921 cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg
    10981 gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa
    11041 taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga
    11101 tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg
    11161 agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct
    11221 atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac
    11281 cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg
    11341 ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tacttcttga
    11401 atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc gtgtacc
//
LOCUS       DASJUF010000086        11446 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJUF010000086 DASJUF010000000
VERSION     DASJUF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.086"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..53)
                     /locus_tag="U4B62_002092"
     CDS             complement(<1..53)
                     /locus_tag="U4B62_002092"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN2142237.1"
                     /translation="MLVFQMRNVDKTSTVLK"
     gene            complement(102..341)
                     /locus_tag="U4B62_002093"
     CDS             complement(102..341)
                     /locus_tag="U4B62_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN2142238.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            complement(475..1692)
                     /locus_tag="U4B62_002094"
                     /pseudo
     CDS             complement(475..1692)
                     /locus_tag="U4B62_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            2183..3121
                     /locus_tag="U4B62_002095"
     CDS             2183..3121
                     /locus_tag="U4B62_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2142239.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            3251..3898
                     /locus_tag="U4B62_002096"
     CDS             3251..3898
                     /locus_tag="U4B62_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HEN2142240.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(4591..5529)
                     /locus_tag="U4B62_002097"
                     /pseudo
     CDS             complement(4591..5529)
                     /locus_tag="U4B62_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(5529..6146)
                     /locus_tag="U4B62_002098"
     CDS             complement(5529..6146)
                     /locus_tag="U4B62_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2142241.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(6160..6330)
                     /locus_tag="U4B62_002099"
     CDS             complement(6160..6330)
                     /locus_tag="U4B62_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142242.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(6678..8168)
                     /locus_tag="U4B62_002100"
     CDS             complement(6678..8168)
                     /locus_tag="U4B62_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2142243.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(8211..8510)
                     /locus_tag="U4B62_002101"
     CDS             complement(8211..8510)
                     /locus_tag="U4B62_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142244.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8876..9160)
                     /locus_tag="U4B62_002102"
     CDS             complement(8876..9160)
                     /locus_tag="U4B62_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142245.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(9679..10164)
                     /gene="vanZ"
                     /locus_tag="U4B62_002103"
     CDS             complement(9679..10164)
                     /gene="vanZ"
                     /locus_tag="U4B62_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN2142246.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(10317..11228)
                     /locus_tag="U4B62_002104"
     CDS             complement(10317..11228)
                     /locus_tag="U4B62_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN2142247.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3508 a         2004 c         1743 g         4191 t
ORIGIN      
        1 tgtttcaaaa cagtagatgt tttatctaca ttacgcattt ggaataccaa catgacgaat
       61 ccctccttct taattacaaa tttttagcat ctaatttaac ttcaattcct attatacaaa
      121 attttaagat aatgcactat caacacactc ttaagtttgc ttctaagtct tatttccata
      181 acttctttta cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtca
      241 gcacgaacac gaaccgtctt atctcccatt atatcttttt ttgcactgat tggtgtatca
      301 tttcgttttt ctttttgtgc gcctaaattt cccacaatca ctcacttctt tctatttctt
      361 cttattctta ttttatcatc aacaatcaca aatcacttgt gatttgtgat aagtgatttg
      421 tgataagtga tttgtgatta atatataaaa gccctcttta aagggctttt atgtttattt
      481 tgagaaagat ataaaatcaa tatatccctt ttccccaatt tttacaacgg cattgtaggg
      541 ctttttctct ttgtttttga ttccttttac cagggtttct tttccctcca gtaattcttt
      601 tacattcgtt ttggttagtt ttttctttct aaaatgttca gctaaagtaa acttacattc
      661 aggataattt gaacaaccat aaaacgattt ttttaataca atattgttgc cacacttagg
      721 acattttcct acaatacttt tttctgcttc tttttctttc tgttcctggt aatcagaaaa
      781 atttagtttt tctatatcgt taggtacagc ttccagtaaa tgaacaatga attttttgat
      841 attcgtaata aagttctctt gattgccttc tcttttaccg atttttttta aatacgtttc
      901 ccatttagcc gtcatttcag cactcgttaa aaggtgctga ctttcaactg cctggcacaa
      961 taattttcct ttttcagtta caacaagctt attcttaatc acttggatat attctttttg
     1021 ttttaacgct tcaataatac ttgctcttgt cgcttctgtt ccaatcccct caacttcttg
     1081 taaaatcttg attgcttctt catcatcaac cgttttattc gccgttttca tagcagttaa
     1141 taatgtaccc tctgtaaaag cttttggcgg ttgcgtttct ttttcaacac tcttaacacc
     1201 aacttcggca cgttcgccaa tgataacgag tggtaacgtt tgaacgtcct ctttttcttc
     1261 tttggtttgt tgtttgaaaa gaattttcca cccttcttgc tttggtgtct ttccgatcga
     1321 ttgaaacagc aaatcagcta ctttcgtttg tatcttggtt tcttcataca agtaatcagg
     1381 taaaaacatg gcaacggtcg ttttaacgac taaagcataa atttttcgtt gtaaatcgtc
     1441 cattttcgct aatgcagatt cggtaggtac ttgttttgtt gggataatgg cgtggtgttc
     1501 ctgtacctta ctaccgtcca cataacgctt tctaggctct gtttgaacca tttcaagatc
     1561 aagtcctaaa aaaccgctat atttgccaaa attcgctttt aaataagcaa attcgttctc
     1621 tgtaataaat ggtgtatctg ttcttggata actcaataat tttgcttcat acagtccttg
     1681 catagctttt aaacgtaaaa ttttgcgttc taagacgttt taaatatatc tgcgtatatt
     1741 tttatgtgtt ttatcttttc gtcggcaaat gcgttaaaat ttgcgaaata aaatagctca
     1801 agggcgaacg cagtgagtgc atttaccctt gatctatttt tttgagtaaa ttagcttttg
     1861 aaaccgacgg aaaggaaaca caaaactttt cagaggcaag gggtttttaa ggggcgcttt
     1921 tagccccttg aatttatttg ctttaagata tatttagttt taaaatagtt ttaacgaaaa
     1981 aaagacataa aaaaagacga gcaacgctac caacgttctc gcccggaata acaaaccaca
     2041 cttgttattc attcgatgat tagtatatca atttttgatt ttgaatacaa aacaaaatgg
     2101 acgaatggat acaggcgaaa tttattttgc gattccactc gtcctttttt cgttccaaaa
     2161 attattttag gagcgataaa aaatgaagaa aattgatgaa aaattagctg cagaaatctc
     2221 actaaaaaaa ccggccgtct ctaatagccg gttaagtaat agccggttaa gtggtcaaac
     2281 tttgggaaaa tctcaacccg cattaagttt tgatgccatg acaatcgttg gaaatttgaa
     2341 caaaactaat gctaaaaagc tatctgactt tatgagtgta gagccacaaa tacgactttg
     2401 ggatatactt caaacaaagt ttaaagctaa ggcacttcaa gaaaaagttt atatcgaata
     2461 tgacaaagta aaagcagata cttgggatag acgtaatatg cgtgttgaat ttaatcccaa
     2521 taaactcaca catgaagaaa tgatttggtt aaaacaaaat attatcgact acatggaaga
     2581 tgacggtttt acaagattag acttagcttt tgattttgaa gatgatttga gcgattacta
     2641 tgcaatgact gataaagcag ttaagaaaac tgttttttat ggtcgtaatg gcaagccaga
     2701 aacaaaatat tttggtgtcc gtgatagtga tagatttatt agaatttata ataaaaaaca
     2761 agaacgtaaa gataacgcag atgttgaagt tgtgtctgaa catttatggc gtgtagaagt
     2821 tgaattaaaa agagatatgg ttgattactg gaatgattgt tttaatgatt tacacatctt
     2881 gaaacctgcg tgggctactt tagaaaaaat taatgagcaa gctatggttt atactttgtt
     2941 gcatgaagaa agtatgtggg gaaagctaag taagaatact aagactaaat ttaaaaaatt
     3001 gattagagaa atatctccaa ttgatttaac ggaattaatg aaatcgactt taaaagcgaa
     3061 cgaaaaacaa ttgcaaaagc agattgattt ttggcaacgt gaatttaggt tttggaagta
     3121 aaataagttt tatttgataa aaattgctaa ttcagtataa ttaatattta cgaggtgaca
     3181 taacgtatga aaaaatcaga ggattattcc tcctaaatat aaaaatttaa aatttaggag
     3241 gaagttatat atgactttta atattattga attagaaaat tgggatagaa aagaatattt
     3301 tgaacactat tttaatcagc aaactactta tagcattact aaagaaattg atattacttt
     3361 gtttaaagat atgataaaaa agaaaggata tgaaatttat ccctctttaa tttatgcaat
     3421 tatggaagtt gtaaataaaa ataaagtgtt tagaacagga attaatagtg agaataaatt
     3481 aggttattgg gataagttaa atcctttgta tacagttttt aataagcaaa ctgaaaaatt
     3541 tactaacatt tggactgaat ctgataaaaa cttcatttct ttttataata attataaaaa
     3601 tgacttgctt gaatataaag ataaagaaga aatgtttcct aaaaaaccga tacctgaaaa
     3661 caccataccg atttcaatga ttccttggat tgattttagt tcatttaatt taaatattgg
     3721 taacaatagc agctttttat tgcctattat tacgataggt aaattttata gtgagaataa
     3781 taaaatttat ataccagttg ctctgcaact tcatcattct gtatgtgatg gttaccatgc
     3841 ttcactattt atgaatgaat ttcaagatat aattcatagg gtagatgatt ggatttagtt
     3901 tttagatttt gaaagtgaat ttaattttat acacgtaagt gatcataaaa tttatgaacg
     3961 tataacaacc acattttttg gttgcttgtg gttttgattt tgaatttggt tttgaactta
     4021 tggactgatt tattcagtcc attttttgtg cttgcacaaa aactagcctc gcagagcaca
     4081 cgcattaatg acttatgaaa cgtagtaaat aagtctagtg tgttatactt tacttggaag
     4141 atgcaccgaa taaaaaatat tgaagaacaa ctagcaaaag attttaaaga gttattttat
     4201 tttaagtctt tataacatga gtgaagcgaa tttttaaatt tcgatagaaa tttttacatc
     4261 aaaaagcccc ctgtcaaaat tgacgaaggg ggttttttgg cgcacgcttt tcgttagaaa
     4321 tatacaagat tgaaaatcgt gtataagtgc gccctttgtt ttgaacttag cacgttacat
     4381 caatttttta aaatgatgta taagtgcgcc cttttaaatt ttgagtgatt atattttttg
     4441 agttagaaaa agggattggg aaaatttccc aaaataattt aaaaaataag caaaaatttt
     4501 cgatagagaa tgtgctattt tttgtcaaag gtgtatacct tgactgtgct tgctgttaca
     4561 ttaagtttat ttttaagtta ttaaaaaaga aatagctttt aaagtttggc tcgctgtcgc
     4621 tttataaagc tgattgactt ttgattgcaa actacttaaa gaaaacaaac tcggactatt
     4681 cgttttcttc tctttggttt gaacatcagc aattatcccc tcttgattgc ctattttagc
     4741 ttgtttagaa gaaacaaaag ctaaaagctc ctcttgggtt ttaaaacgct gtgtggggct
     4801 tagaacgccc ttaaacgacc cttggtttac ttttatacta gcttccacct cgaaaaaagg
     4861 ttctttttta aaattctcta tggcttcctg gcgctgaaaa ataaggtata aggtgggcgt
     4921 ttgaacacgt cctagtgaaa atgtaccttg tacgcccttc tgttgtaaat ttaacgtata
     4981 caaagggctt gcgttcatgc cgatcaacca atcggcaatt tggcgtgttt gcgcttcttg
     5041 ataaaaggga tagtaattca ttccaggttg caaattttga aaaccgcttc ggattacatc
     5101 tttttctaag ctattgatcc atagtctttt aaatgtttta tcttttgaaa aggcatttgc
     5161 tttatggata atcgaccagg cgatattttc accttctctg tcgctatctg ttgcaacaat
     5221 aattgtattt gcctttttga gaagttctgc aacaatttta aactgctttc ccttatcttt
     5281 tgcaacttca aaatcgtatc gatcaggaaa aatcggcaaa gattcaagtt tccaattttg
     5341 ccacttttcg tcataatgac ctggttctgc taattccact aaatgcccaa aaccaaaggt
     5401 gataaacgtt tcatctgtaa atagtgggtc tttgatctca aaataaccgt cttttttggt
     5461 gctttgtttt aaagcacttg cgtaggctaa tgcctggctt ggtttttcag ctaaaataac
     5521 cgtactcatt aactatccct cttttcattg ttttttcttt gatcgactgt cacgttatat
     5581 cttgctcgat accttctaaa cgttcggcga ttgattccag tttgttcttc aacttcttta
     5641 tcggataaac cattcaaaaa caaatcgaaa gcatgcttta aacgtggatc attttcttta
     5701 aatttatgct gacgaccttt gaatttgcct tttttcttag caatttcgat tccttgtgcc
     5761 tgacgttcct taattttttt tcgttctgat tctgcttgat acttgtacaa ttcaatgaca
     5821 aggctattaa tcaaacgcct taaattttca tcttcaatac cattcattga gggtaaattt
     5881 aagacttcca gggttgcccc cttaatttga atttgattca tcaattctgt taattcttta
     5941 ttatttcgtc ctaatcgatc taattcagta acaataacaa tatccccttc acgaatatag
     6001 ttaagcatag cttgtaattg tgggcgttcg accgattgac cgcttaattt gtctgaaaag
     6061 accttagaaa cgccctgtaa cgcttgtaat tgccgatcta agttctgttc tttgctactg
     6121 acacgtgcat aaccaatttt agccattttc aaccaacctc taaaattctc tcggttgcaa
     6181 taaccaatca gcaatatcta ctttttcaat ttcaaattgc ttatcagaaa ttgtcttttc
     6241 gtaagcgata aaatcttgcg catattgttg ctcattaaaa atagccacca cttcgtcatt
     6301 ttctaaaact cgataaataa attttttcat tttactcctc ctattatgcc caacttaaat
     6361 gacctattca ccaagtcaat tatactgcta aaatcatatt aggacaaata ggtatactct
     6421 aatgacctat aaaagataga aaattaaaaa atcaagtgtt cgcttcgctc tcactgcccc
     6481 tcgacgtttt agtagccttt ccctcacttc gttcagtcca agccaactaa aagttttcgg
     6541 gctactctct ccttctcccc ctaataatta attaaaatct tactctgtat atttctgcta
     6601 atcattcgct aaacagcaaa gaaaaaacaa acacgtatca tagatataaa tgtaatggca
     6661 tagtgcgggt tttattttca gcctgtatca tagctaaaca aatcgagttg tgtgtccgtt
     6721 ttagggcgtt ctgctagctt gtttaaagtc tcttgaatga atgtatgctc taagtcaaaa
     6781 gaatttgtca gcgcctttat atagctttct ttttcttctt tttttacttt aatgatcgat
     6841 agcaacaatg atttaacact agcaagttga atgccaccat ttcttcctgg tttaatctta
     6901 aagaaaattt cctgattcgc cttcagtacc ttcagcaatt tatctaatgt ccgttcagga
     6961 atgcctagca cttctctaat ctcttttttg gtcgtcacta aataaggctt gtatacatcg
     7021 cttttttcgc taatataagc cattaaatct tctttccatt ctgacaaatg aacacgttga
     7081 cgttcgcttc tttttttctt gaatttaaac cacccttgac ggacaaataa atctttactg
     7141 gttaaatcac ttgataccca agctttgcaa agaatggtaa tgtattccct attagcccct
     7201 tgatagtttt ctgaataggc acttctaaca attttgatta cttctttttc ttctaagggt
     7261 tgatctaatc gattattaaa ctcaaacata ttatattcgc acgtttcgat tgaatagcct
     7321 gaactaaagt aggctaaaga gagggtaaac atgacgttat tacgccctat taaacccttt
     7381 tctcctgaaa atttcgtttc gtgcaataag agattaaacc agggttcatc tacttgtttt
     7441 ttgccttctg taccgcttaa aaccgttaga cttgaacgag taaagccctt attatctgtt
     7501 tgtttgaaag accaatcttg ccattctttg aaagaataac ggtaattagg atcaaaaaat
     7561 tctacattgt ccgttcttgg tatgcgagca ataccaaaat gattacacgt tagatcaact
     7621 ggcaaagact ttccaaaata ttctcggata ttttgcgaaa ttattttggc tgctttgaca
     7681 gatttaaatt ctgattttga agtcacatag actggcgttt ctaaaacaaa atatgcttga
     7741 taacctttat cagatttgat aatcatagta ggcataaaac ctaaatcaat agcggttgtt
     7801 aaaatatcgc ttgctgaaat agtttctttt gccgtgtgaa tatcaaaatc aataaagaag
     7861 gtattgattt gtcttaaatt gttttcagaa tgtcctttcg tgtatgaacg gttttcgtct
     7921 gcatacgttc cataacgata aacgttgggt gtccaatgtg taaatgtatc ttgattttct
     7981 tgaatcgctt cctcggaagt cagaacaaca ccacgaccgc caatcatgct tgattttgag
     8041 cgatacgcaa aaatagcccc tttgctttta cctggcttgg tagtgattga gcgaatttta
     8101 ctatttttaa atttgtactt taacaagccg tcatgaagca cagtttctac aacaaaaggg
     8161 atattcattc agctgttctc ctttcctata aatcctataa aataggttgt ttaattaact
     8221 tggtttgctt tttcattcaa ctgtttcaat attgcatgtt ttgaaaaaga tttttttcct
     8281 ttataagtca atttttttcc actaatcgaa taaattattt tgttattttc tattaactta
     8341 tatatataat cttccccctc cgaagaaaaa tacttatctg attttgtttc taagtagata
     8401 tttctctttt ctaactcttt cttaaacgtt tctagtgtat agatatttgc taattttctt
     8461 atctccaata aactattttt tatataagtt ttacattcat catgattcat acaaactcca
     8521 ccttctataa atgaatacaa aaaaagcaat caaacgattt ccgattgatt gcttaacaat
     8581 tcttaaattc agtagcttag atacttgaaa actctctgat ttccctatat aatgatagta
     8641 cggttatata ccgtcttcaa acaaagttaa ttaaataact tcttacgagg gaagagttca
     8701 tctgactaac tgataagcgt tggtttggca atcttatcgg gctatgcatt tataaaatgt
     8761 cgtcaaacat tttataaatg tgtcatggct cttttttcgt ttctattcag ttcgttgttt
     8821 cgttatatct agtataccgc ttttaaaaaa aataagcaac gatttcgtgc attattcaca
     8881 cgaagtcatt gcttttttct tcttccattt ctaaatccaa tgttacttgt tctgattctg
     8941 tttctggttc tggttctgtt ggctcatttg ggattaaatc cactactagc gttgagttag
     9001 ttaactttgc aatttgttct agtgttttta tggttggatc tgattttcct gattctattc
     9061 gtgaataatt tgatctactc atttctaatt cttgggagaa tttttctttt gatttggtac
     9121 ctctcatctt ttttaagctt tctctaaatg ctagttccaa gtttctcccc cctctttttg
     9181 tgtaataaaa ttcacgcttt atttaaatta tagcttttaa tatcttaata aacaagtgat
     9241 taatcacttg tgatttgtga ttattccaaa agtaggtaat ttcaccaacc tttttcaaaa
     9301 gtttttatcg acgattattg gactggatcg tgtttcctaa agcgattcta agcgcatgtt
     9361 taattttaaa gtgggtaatt ctctttaaaa cgtcctacaa agattcaaaa aaagcatggc
     9421 tgctagtggg ggtaccgtca ggaaaatgcg gatttacaac gttaaggaaa tttattaaag
     9481 agaccaattc ctcagcacta aaattgataa ttgggctttc tttgttacta tagtgttagg
     9541 taagcttttt actttatcac cggttttccc ctcacttcac acctacgggc gagtttcccc
     9601 acatcccgtt ttccatctaa tccaattcat tcaatatctt tatgtgtatg aaagatcaga
     9661 ttgcttgatt tagacatctt acacgtaatt tattcttaaa tgggtacggt aaacgagcaa
     9721 taatacgagc aaaagtatac ctacaaaaat aataactctg tctaattttt tttgattcat
     9781 atgcttattg cttaaaccat ataatttcag tccaagaaag cctccaacag tatttgtaat
     9841 tacatctgtt atgtctgtcg ctccaatagc gaagataaat tgaattattt caaaagtaag
     9901 acttaaaacc agtacaaaag caaacttagg taaaaatccg atttctttaa aattgacatt
     9961 caaaagcaag ccaaatggaa taaagattat aacattatct atcatctctc tgaaattccc
    10021 agtagcagta aatggagtca agttaagact tctttgatga taattaaata ctgataaaat
    10081 attgtattgt aatttgaata acactaacca gattagtgtc actaaatata aagctagcaa
    10141 tcctctagat aatatttttc ccaatataat ttcctctaat tcttttgttt aagttgtatc
    10201 attagtatag caatgtccta gttaacgtgg gttttaaatt aaaaagctaa cgttacccca
    10261 aaagtgctag tacaataatg ctgttcatta atttcgttgg tttcattccg ccatccttac
    10321 ctccttgaat tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg
    10381 tttcctgata tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca
    10441 ggataataaa agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct
    10501 tttaggtaat ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt
    10561 ggtaaaccaa catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg
    10621 tcctctggat aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct
    10681 tcaggggctc gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta
    10741 tgctcactat aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt
    10801 tgctcatcaa agtctcgata gccactatta ataataaaat gactaacgcc accctttaca
    10861 gcatcattga ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta
    10921 tcaagcaacc cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac
    10981 ttcacacttt cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa
    11041 acctgttctt ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa
    11101 tgttctttgg gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct
    11161 tcattaacgt agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac
    11221 ttcttcattt tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat
    11281 cttcccctta aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag
    11341 tcaattattt cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt
    11401 gatacccatg ctgaatttaa cgaggctgaa ctgcctgttg acaatg
//
LOCUS       DASJUF010000087        11394 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJUF010000087 DASJUF010000000
VERSION     DASJUF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.087"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..114
                     /locus_tag="U4B62_002105"
     CDS             <1..114
                     /locus_tag="U4B62_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2142248.1"
                     /translation="FISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(148..1377)
                     /gene="hflX"
                     /locus_tag="U4B62_002106"
     CDS             complement(148..1377)
                     /gene="hflX"
                     /locus_tag="U4B62_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN2142249.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1379..2296)
                     /gene="miaA"
                     /locus_tag="U4B62_002107"
     CDS             complement(1379..2296)
                     /gene="miaA"
                     /locus_tag="U4B62_002107"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN2142250.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2300..3037)
                     /locus_tag="U4B62_002108"
     CDS             complement(2300..3037)
                     /locus_tag="U4B62_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2142251.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(3128..3655)
                     /locus_tag="U4B62_002109"
     CDS             complement(3128..3655)
                     /locus_tag="U4B62_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142252.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3835..4428
                     /gene="clpP"
                     /locus_tag="U4B62_002110"
     CDS             3835..4428
                     /gene="clpP"
                     /locus_tag="U4B62_002110"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN2142253.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4532..5017)
                     /locus_tag="U4B62_002111"
     CDS             complement(4532..5017)
                     /locus_tag="U4B62_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN2142254.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5170..5367)
                     /locus_tag="U4B62_002112"
     CDS             complement(5170..5367)
                     /locus_tag="U4B62_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142255.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5462..7222)
                     /gene="efrB"
                     /locus_tag="U4B62_002113"
     CDS             complement(5462..7222)
                     /gene="efrB"
                     /locus_tag="U4B62_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN2142256.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7219..8949)
                     /gene="efrA"
                     /locus_tag="U4B62_002114"
     CDS             complement(7219..8949)
                     /gene="efrA"
                     /locus_tag="U4B62_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN2142257.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9342..9554
                     /locus_tag="U4B62_002115"
     CDS             9342..9554
                     /locus_tag="U4B62_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN2142258.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9556..11235
                     /locus_tag="U4B62_002116"
     CDS             9556..11235
                     /locus_tag="U4B62_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2142259.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3314 a         2459 c         1988 g         3633 t
ORIGIN      
        1 ttcatctcat ccgttgcgtc caaaagaaac catatcccta gaaaatcact aaattaccaa
       61 acaccattag aagttttttt gagttacgta aatggaaagt tttgtctcgc ttaatttgac
      121 aaataatatt gttaaaaaaa taaaatttta ttggtttttt cttaaccatt ttgaattggt
      181 ttttgcaaac cctttaactt catattcgtt tgtttcttcc atgtacgctt cgtccaaaac
      241 cattgtttgt cttctcaact cgcttaattt ctgtccttcg ttgcttggca cagaaagtgt
      301 ataaggggtc attagttcca tcagctgacg tttgattgct tcgatcaacg ccatcttacc
      361 ttctttcgtc tgagtagaaa tcaatacatt tgggaatagc gtaggcgtga acatcgctgg
      421 gtcgatctgg tctgccttat tgtaaacagt cagtatcggc atttctttca tattcagttc
      481 atccatcaat tttaagacag tctgctcctg taaaatacga tctggagaac tagcatcgac
      541 tacatgtagc aaaagatcca tattttgact ttcttctaat gtagaatgga aagcatcgat
      601 cagttgcgta ggcaggtctt ggatgaaccc gacggtatcc gttacagtaa tctcaaatcc
      661 ttcagcaaaa cgccatctct tagtcaatgg atctaacgtt gcaaacaatt gatctttcga
      721 ataagtatcc gcttgtgtca atagattcaa gatagttgac ttcccggcat tcgtgtagcc
      781 aataagtcca atctgaaaaa tctcgctatt ttttcttttt tggcgattgc gctctcgatg
      841 agcttctact tctttcaatt ctttcttaac agcaaaaatt ttattccgta tatgtcggcg
      901 gtctgtttct aactttgttt cacctggccc tcttgttccg atcccaccgc caagacgaga
      961 taatgttttc ccttgcccta ctaaacgagg cagcaggtat tctaactgag caagttcaac
     1021 ctgcagcttc ccttctttcg aacgagcacg catcgcaaaa atatctaaga taagctgcac
     1081 gcgatcaatt accggaagac caacagcatc ggtaatcagt tggctctgtc tcggagttag
     1141 ctcatgatta aagatgatca aatccgcttc atgcgcatcc gcttgttgaa ctaattcttg
     1201 taatttccct ttaccgataa tcgtttgccg atccacttgt ggtctctttt gtgtaattgt
     1261 gaatacgact tctcctgatg cggtattcgt cagctgaact agctcttcca ttgactctga
     1321 aaagtaacgc tggtttgctt cggtttctac ccctacgatg atcacttttt cttccatttt
     1381 attccgcctc cagccatttt ttgattgttt gctcaatctg acttttttga tttggctcac
     1441 ttataagatc aaaccactgt gcatccaaac ggttacgaaa ccacgtcaac tgacgtttcg
     1501 cgtatcttct cgaatgtagt ttgacttgtt ctgttgcttc ttccaatgtt tctttccctt
     1561 caaaataagg gaaaaattct ttatatccaa taccttgact ggcctggact tctccaagct
     1621 catacacctt acgcgcttct gctactaaac cttctgctag catcaaatcg actctttgat
     1681 tgattcgttc ataaagaata ctacgcttcg tatctaatcc aatcatgagg taatcataca
     1741 attgctttgg ctcttcttta ggtgccgtga tactatgtcc tgtcgtctct aaaacttcca
     1801 gagctcttat cactttgcgt tgattgttcc agtggatttt ctcagcagct gccgggtctt
     1861 tttctttcaa ctgattccat aatgcttggg aaccaattgt ttcagctaac gtttcatact
     1921 tttttctaat ctcaggattc gcttcttctg tcgctcctaa ctgataatca tatagaaggg
     1981 attggatata gagacccgta ccaccagcta tgataggtag gtgccctcgg ttcgcaatct
     2041 cggtaatcaa ttgtcgacct gaatgctgaa aatcaacggc tgagtaacgt tgagtaggtt
     2101 cgatcacatc aatcaaatga tgcggaacat tattcatctc ttcttctttg atttttgctg
     2161 taccgatatc cagattttta tagatttgca atgaatcacc actgatgatc tctcctccaa
     2221 atagttgtgc gagttccaca cttagcgctg ttttaccgac tgctgtcgga ccgacgatca
     2281 ctaatacttt ttccatattt tacctacttt tcgttgattg tttccaccga agagctttct
     2341 ccggaaaatc agttattatt ccatctattt tgagttcttg acatttggcc atttcttctt
     2401 catcgttaat cgtccatgca cgaattgctt ttggaaaatc agctgcctgg actggatgtt
     2461 cgaacaacca agtatttttt ggatgtactc cttctatata aggggtttct gctgctagtt
     2521 ggatcttttt atcagaagta ctcatcaata agtccagctg tatctccggt tccagtaatg
     2581 cgattttttc caaagaatcc aaatgaaagc tgcaatatgc atgttgaaaa ggccaatctc
     2641 tactttttaa tgtttcggct aatttttctt cgataccggg atattcatac tgatcagttt
     2701 taatttcaat cattagcatc cctcggtaat ttttttggag gagcagtcct aacacttctc
     2761 tcaattcagg aactttagct gctggaaatt ctttgctgaa tttgccccct gcattcaatt
     2821 ttttgagttc ttccaatgtt ttttctcgaa tcagcccttt accgttagtc gtccggtcca
     2881 cttcttcgtc gtgcataacg atcaaataac catctttgct tagatggaca tctagttcga
     2941 taccatctgc acctactcgt acagcttctg caaatgcagg aagcgtattt tctgggtggg
     3001 tgccgctgct tccgcgatgg gcatagattt gtaacatctt tcttcactcc gttcagttct
     3061 attgtatcat taagaaaaaa agaaagcatt accacgctct catgtgcaac ggcaatgctt
     3121 caaaatatca gctccaaaca tcatctttcg agaagaaacc aatcggctgg cgcgtcatag
     3181 tcttcaactg agcttttttt tcattgatca tttcagtcag actatccgta aaattctcta
     3241 aaatcacgcg tccgccttta taagcatatc caccaatctt cattgtttca tctggagata
     3301 aaatcacttt tcgatgggag atattcttaa atagctcgtc taaaatataa tttggcagat
     3361 aaaaactatt ttgcgctgaa aatgattgga gatacgcaaa atcgtcaatc gtgatataac
     3421 aatcatcaaa tgcatcaaag ctgacatatt cttccatcag cgttgtccgt ttttgcaaga
     3481 tctcgttgat ctttttattt aattgattca gcgtctcgat ttctttgact ttcaattcca
     3541 attcacgtct acgctgttct ttattttcca agtgacgcat ttgcagtatc atgccgatat
     3601 agacaactac agcacctgtc agcactccga taaggccagt aataccaact atcaatctgt
     3661 tgttcctcct tgttttgttt ctctgcttat cataccataa agcgggttta tgcgactaat
     3721 ttgcctaagt tttttatttg accttttttg accaaaaatg tatactacat tttaggtatt
     3781 agaaactaaa ttatggtaca ctatttctaa acgaaatcaa ggaggaactc tattatgaat
     3841 ttaattccta cagtcattga acaatcttca cgaggtgaaa gagcttacga tatctactca
     3901 cgtttattga aagaccgtat catcatgtta agcggccaag taacagatga tcttgctaat
     3961 tcgatcatcg cccaattgct tttcttagac gctcaagatt ctgaaaaaga tatctatctt
     4021 tatatcaatt ctcctggtgg ttcagttaca gcaggtatgg ctatttacga cacaatgaac
     4081 tttgtcaaag cagatgtcca aacaattgtt atgggtatgg ctgcttcaat gggaagcttc
     4141 ttgctgacag ctggtacaaa aggtaaacga tttgctttac caaatgctga gatcatgatc
     4201 catcaacctc ttggcggcgc tcaagggcaa gcaactgaaa tcgaaattgc ggcacgccat
     4261 attttgcaaa cacgcgaacg cttaaacaaa attttagctg aacgcactgg acaaccgctt
     4321 gaagtgattg aaaaagatac agatcgtgac aactatatga cagctgaaca agcaaaagca
     4381 tatggattga tcgatgaagt catggaaaac agttcttcat taaattaatc tgtttcaaag
     4441 gaagcttttt taagctctct tatctaaacc aaataaaaaa ggatggtcaa ctcaaactga
     4501 ccatcctttt ttattttatg gatgctactg cctatctttg ctccttgaat tttccttgcg
     4561 catgcatccg acctatctcg acataatgct ggatgttttc tttgtttttt tgtatttctt
     4621 cttttgtcaa aggacggatc actttagctg ggcgaccaaa agccaagaca ttaggaggaa
     4681 taacttttcc ttctgttact aatgatccag caccaatcaa actattctca ccaataaccg
     4741 catgattcaa tatcgtggtc cccatcccga tcagcgcccc tttttcgatt gtacagccat
     4801 gtagcatgca ctgatgtcct accgtcacat tctccgcaat atctacaggt gcatcatgat
     4861 cgacatggat gattgtacca tcttggatat tcgttctttg tcctattttt atccaattac
     4921 tgtccccgcg taaaacagct tgataccaga tcgtcacatc ttctgacaat gtcacatcac
     4981 caatcactgt ggcatttgat gctataaacc gttccatatt tatttttctc ctttcatttt
     5041 ttgtagaaac taatcctatt gaataaaggt atcatagcat atatttattt cataagaaaa
     5101 aaaacaggta aaacctgctc aggccagatg tctatttaca gtatctggta actgacgttt
     5161 tacctgtttt tattgtacat ctatattttc ttctattact ttttttaaaa ttaattcgta
     5221 cacttctatt tgtgctgcca agcgttcgat ttgaaactca ttttcaaaaa aatcagctga
     5281 atcaaaaggc ttcgtctgac agacgtgcgt taaacgatca acttctcttt tggcttcagt
     5341 aattttttca tttaaaaact gttccatctc cgtgtcctca cttttcttga taactttact
     5401 ttaccattat caagaataga gaacaaaaaa taagttttaa actgttttat tcttatatga
     5461 cttattctgc ttcctcagca aattggctgt tatacagttt ttcataaaat ccaccttgtg
     5521 ccaataaact ctcatgcgtt cctttttcaa tgatctgacc ttgatccatc actagaatca
     5581 gatctgcatc acggattgtc gataaacggt gagcgatgac gaaacttgtc cgtccctcca
     5641 tcacgcgatc cattgctttt tgaatcaacg cttctaaacg ggtgtctact gaacttgttg
     5701 cttcatctaa aatcaagatt ttaggatcgg agaccactgc tcgggcaatc gtcagcaact
     5761 gtttttgtcc taaagacaca ttgtctccct cagaattgat ggccatatca tatccatctg
     5821 gcatcgttcg gataaaatga tctacgtttg ctgttttcgc agcatcaact acttcataat
     5881 ctgtcgcatc aagctttccg aagcggatat tatctgcaat cgttccttga taaagccatg
     5941 catcttgcaa gaccatacca aatagcgaac gcacatcact gcgactcatt gatttggtgt
     6001 cgattccatc aatccttatt gctcctttgt ctacgtcata aaaacgcatc aatagattga
     6061 tcaatgtcgt cttgccggca ccggtcggac cgaccacagc tacggtttga ccagctttca
     6121 cttcaaaatt caaatcttgg attaatggtt tcttcggatc atatgcaaaa tcgacatgat
     6181 cgaatgtcac atctcctaaa atcgtttctg gcagtgaagc atctactttg ttttcttttt
     6241 cttctggctc atctaaaatc tcgaatacac gtttcgttgc cgctgatgca ctttgcaaaa
     6301 ctgaagacaa ttgtgtcacg ttccccattg gctgactgat ttgccaaata tattggatga
     6361 atgcttgtag ttgaccgaca acgatcgccc cagtaataac ataaaagctt cctagaacag
     6421 ccataccaat ataagtagca taggcagtta attgaactag aggcatcatc aagccagaaa
     6481 tgaatgctgc acgaaaccca aagtatttca agctgtgatt aacttttttg aatccttcca
     6541 atgtttcttt ttcgcgtcca tatactttca atacactaaa acctgtcata ttttcttgga
     6601 cgtagccgtt tagctcacca agagaattct gcatgctttg aaaatatttc tgcgaagcat
     6661 tgatcactcg ttttgagatc cagatcgatg ttggaatcat gatcatagag atcagcgcca
     6721 tcatcggctg gatataaaac atcattgctg cagccaacgt aattcccaat atcgcattta
     6781 cgataccaat aaaagcttgt tgtaaagcac cgctgactgc atctacatca ttcgtcacac
     6841 gagacaaaat atttccttgc tggtttttat cgaaatatga aaccggaaga cgattgattt
     6901 tttcttctat gtctcttctc aaatcatgca tggcactttg aacgacattt gtcatcaaaa
     6961 caccagaaag taattgcgaa atacaatacc ctattccaac aactgtgatc caaatgagac
     7021 atttgaatat ataagaaaaa ttgattgatt cgccattggc aatattacgg ctgatttctg
     7081 tagtaggaag tcctgtaaca tatggcaaag ccacgttgaa agccactgtc agaactgtaa
     7141 ataggatcac taaaagaaaa gtctcacgat acggtctgat atatcgagct aaacgtttta
     7201 aggaagaaaa ggtttttttc atgctaattc ctcctttgat aattgagaag cggcaatatc
     7261 ataataaatc gggcattttt ccaacaactc ccgatgcgtc cccataccga cgacttctcc
     7321 ttcatttaat acgatgatct tatctgcatg catgatcgtt cccacacgtt gtgctacgat
     7381 caatacagta gattctgtcg tttctttttt cagacgagca cgtaacttcg catccgtttg
     7441 ataatctaat gcagaaaagc tgtcatcaaa aatatagatt tccggacggc gtatgatggc
     7501 tctggctatt gccaatcgtt gtttttgtcc acctgaaaaa ttggaaccac cttctgataa
     7561 atgttcatta aatccatctg gttttcgaga aatgaattct gaagcttgag caatctcagc
     7621 tgcacgttcc atttcttctt gcgtagcatc ttctttcccg tatcgcaaat tatctgcaat
     7681 cgttccagta aatagcagcg ctttttgcgg aataaaccca atcttatctc gcaacttgct
     7741 tagccgataa tctctcacgt ctactccatc gatcaagatc tttccttcac tcacatcata
     7801 aaagcgaggg atcaattgga tcaacgtaga ttttccgcta ccggtacttc caataaaagc
     7861 aacggtctct cctggagagg ctgtaaaact gacatttcgt atcactggct cttgcgaatg
     7921 gcctggataa gcaaatgtta cattttgaaa ttcgatatac cctttagttt ttgtatcaga
     7981 tataccattt ggatcttcta caatcgaagg tttggcatct agggcttctt ggatacgtcc
     8041 agctgaaaca gctgctcttg gatacatcat gaacacacta gcaaataaca tgaatgagaa
     8101 caaggcatgg aatatatact cgataaacgc aatcaaatct cctacacgaa gctcgcctgc
     8161 atcgatttgc actgccccgc tccagatgat caataccatt acgatattaa ataagaaata
     8221 aaaacccggt tgtgcaacag ccatcaaacg gaataagctt tttgaactag aagcataatt
     8281 ctcattgact acagcaaaac gtttttcttc aaacttttca ttgacgaatg cacgaatcac
     8341 acgcaatcct gaaagatttt cccgcaagat actgttgatt ttatctaagt ttttttgttg
     8401 ctttttagat aacggttcag acaccttagc aatcgctacg actgccaata atagtaaagg
     8461 taatgcacct actacataca aactgagtgt cacacttgta cgcatgacca tgtacaaact
     8521 gacaatgaac atcattggtg ccataaaacc gatacgtaaa atattttgca ggaacaacat
     8581 gatctgataa gcatcgtttg ttgtacgagt gatcaatgaa gatacgccta gattttcgta
     8641 ttcctgatgc gaataacttt ggatcttttc gaacaaatca tctcgaatat ctgctacgat
     8701 attagtcgta atcctcgaac caaaataacc taacaagata ttcaaaataa taccgattac
     8761 agtaataacg atcataaata tcccttgttg cttcacataa tcaaaatcat cattgcggat
     8821 tcctacatcg atcatacgtg ctaaaatcgt aggaagtcct aattcaatca aaataaaacc
     8881 aaatacgcat atgaaattga aaaacaagaa tttcttataa cgcatggtat aatgccacat
     8941 tagtttcact ttctcatctc cttttaatct tttgttacat tttcattact aaaaatgata
     9001 gcacaacgag cctattaata gaacacttct ctcataaaaa agtcattatg attatagcgg
     9061 gatgataaac attgattttt tctttcaatc tagttgattt ctatacttat ttgctgtttt
     9121 atgctagaat aacaattaat tgttgtatat tttttataca gaaggtacgg ttgaaaaaat
     9181 atccgaacag ataagcaaaa acgaggcttg tctctttgac tcgaatccag tattctagtc
     9241 aaagcatatg aaaaaccagg tggcttgtcc cctcgttttg tgttgtctaa aaaaatcaac
     9301 tagctaaatc actgctttac gcagataaag gagacaaaac aatgatctat aaagtttatt
     9361 atcaagaaac aaaagtacgc aaccctaaac gggaagaaac aaaatctctt tacattgagg
     9421 caaaaagcga tgtagacgct cgccaacgtg tggaagaaaa taccccatac aatattgaat
     9481 tcgttcaact tctagaagga agtcatctag agtacgaaaa agaaaatgct gatttctcat
     9541 tagtggagtt ttaatatgaa actttccatc aaaaataacg agacaggcgt ctttgctatt
     9601 ggtggattag gtgaaatcgg gaaaaatacg tatggggtac aattccaaga tgaaatcatt
     9661 ttgattgatg ccgggatcaa attcccggaa gacgatttac ttggaattga ctatgtcatt
     9721 cccgactaca gctacatcat acaaaactta cacaaagtaa aagccttggt gattactcac
     9781 gggcacgaag accacatcgg cggaataccc tacttgctga aacaagcaaa tattcctatc
     9841 tatgctggtc ctctcgcatt agcgttgatc acaaacaaat tagatgagca tggcttgctt
     9901 cgagatgcag agctccatga gatcaatgaa gatacgatca tccgttttag aaagacttct
     9961 gtcagctttt tcagaacgac ccatagtatc ccagatgcat tagggatcgt ggtgaaaaca
    10021 ccttctggaa atatcgtagc gactggagac ttcaaattcg atttcactcc tgttggcgaa
    10081 ccagcaaact tgcataaaat ggctcgtatc ggggaagaag gagtcttgtg ccttttatct
    10141 gatagtacaa atgctgagat tccaacattt actaaatcag aaaaaacgat tgggcattct
    10201 attttgaaga tcttttcaaa aatcgaagga cgtatcattt ttgccagctt tgcttctaac
    10261 atcttccgtt tgcagcaagc agcagatgcc gctgtggcaa ctggtcgtaa aatcgctgtt
    10321 ttcggacgaa gcatggaaaa tgccatcgtc aacggtcaac gtttaggata tatcaaagtt
    10381 ccagacggca catttgtaga tgcacacgaa atcaatcgct tgcctgccaa tgaaacgatg
    10441 atcctatgta caggttctca aggagaagcg atggccgcgt tgagccgcat cgcgaatggt
    10501 actcaccgtc aaatccaaat ccagccagac gatacagtga tcttttcaag ttcacctatt
    10561 cctggtaata caacaagtgt caatcatttg atcaatttgc tgtctgaagc tggtgcagaa
    10621 gtgattcacg gaaaaatcaa taacatccat acttccggtc acggtggaca agaagaacaa
    10681 aaattaatgc ttcgtttgat gaagccaaaa tattttattc ctgtccacgg agaattccgc
    10741 atgttgaaag tacatgcttc tctagcacaa gatgtaggtg ttcctgcaga aaactgcttc
    10801 atcatggaaa atggcgatgt tttagcgtta actagcgatt ctgcgcgtcc tgctgggcat
    10861 tttgtggctg gagatgtgta tatcgatggt agcgggatcg gtgatattgg taatgtcgtt
    10921 ttacgtgacc gtcacctgct ttcagaagaa ggtcttgtat tagctgtcgc gacagtggat
    10981 atcaagaaaa aagaaatctt ggctggtcca gatattctct ctcgtggatt tgtctacatg
    11041 cgagaatcag gagaaatgat caatgaagct cagcgaatct tattccacgc tttaagagaa
    11101 tgtttgaatg gacctgactg ttcagaatat aaattgaaag aagcgatgac tggcgcgctt
    11161 cagcctttct tattcgaacg tacagaaaga catccaatga ttttgccaat ggtaatgaca
    11221 ccagatgaag aataaataag aggttatgaa atcagcacct tgtcctgaat ataagagaaa
    11281 acaaatgaaa aacatgagaa gccatttttg tttgttctta ttccttgata caaggtggct
    11341 ctttcatagc ctcttaaatt tatttacttt ttcatacaaa aaaatacctg ccac
//
LOCUS       DASJUF010000088        11198 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJUF010000088 DASJUF010000000
VERSION     DASJUF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.088"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..906)
                     /locus_tag="U4B62_002117"
     CDS             complement(13..906)
                     /locus_tag="U4B62_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2142260.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="U4B62_002118"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="U4B62_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN2142261.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="U4B62_002119"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="U4B62_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN2142262.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="U4B62_002120"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="U4B62_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN2142263.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="U4B62_002121"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="U4B62_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN2142264.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="U4B62_002122"
     CDS             complement(6343..7062)
                     /locus_tag="U4B62_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN2142265.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="U4B62_002123"
     CDS             complement(7059..8372)
                     /locus_tag="U4B62_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2142266.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="U4B62_002124"
     CDS             complement(8453..8773)
                     /locus_tag="U4B62_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2142267.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="U4B62_002125"
     CDS             complement(8881..9168)
                     /locus_tag="U4B62_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142268.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="U4B62_002126"
     CDS             9295..9939
                     /locus_tag="U4B62_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN2142269.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="U4B62_002127"
     CDS             complement(9998..10705)
                     /locus_tag="U4B62_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2142270.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="U4B62_002128"
     CDS             complement(10925..11095)
                     /locus_tag="U4B62_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142271.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DASJUF010000089        11179 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJUF010000089 DASJUF010000000
VERSION     DASJUF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.089"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U4B62_002129"
     CDS             6..305
                     /locus_tag="U4B62_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2142272.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U4B62_002130"
     CDS             378..1739
                     /locus_tag="U4B62_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN2142273.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U4B62_002131"
     CDS             1761..2021
                     /locus_tag="U4B62_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142274.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U4B62_002132"
     CDS             2035..2787
                     /locus_tag="U4B62_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN2142275.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U4B62_002133"
     CDS             complement(2881..3486)
                     /locus_tag="U4B62_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2142276.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U4B62_002134"
     CDS             complement(3539..4513)
                     /locus_tag="U4B62_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN2142277.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U4B62_002135"
     CDS             complement(4891..5610)
                     /locus_tag="U4B62_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2142278.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4B62_002136"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4B62_002136"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2142279.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U4B62_002137"
     CDS             7161..8558
                     /locus_tag="U4B62_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2142280.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U4B62_002138"
     CDS             8639..9079
                     /locus_tag="U4B62_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2142281.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="U4B62_002139"
     CDS             9227..9553
                     /locus_tag="U4B62_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2142282.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="U4B62_002140"
     CDS             9554..10303
                     /locus_tag="U4B62_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142283.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="U4B62_002141"
     CDS             complement(10383..10838)
                     /locus_tag="U4B62_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2142284.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3550 a         1957 c         2295 g         3377 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaactt
//
LOCUS       DASJUF010000090        11118 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.090, whole genome shotgun sequence.
ACCESSION   DASJUF010000090 DASJUF010000000
VERSION     DASJUF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.090"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            80..1339
                     /gene="lysA"
                     /locus_tag="U4B62_002142"
     CDS             80..1339
                     /gene="lysA"
                     /locus_tag="U4B62_002142"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN2142285.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1326..2126
                     /gene="dapF"
                     /locus_tag="U4B62_002143"
     CDS             1326..2126
                     /gene="dapF"
                     /locus_tag="U4B62_002143"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN2142286.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2129..3010
                     /locus_tag="U4B62_002144"
     CDS             2129..3010
                     /locus_tag="U4B62_002144"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN2142287.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3007..3723
                     /locus_tag="U4B62_002145"
     CDS             3007..3723
                     /locus_tag="U4B62_002145"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN2142288.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3729..4424
                     /gene="dapD"
                     /locus_tag="U4B62_002146"
     CDS             3729..4424
                     /gene="dapD"
                     /locus_tag="U4B62_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN2142289.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4440..5441
                     /locus_tag="U4B62_002147"
     CDS             4440..5441
                     /locus_tag="U4B62_002147"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN2142290.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5438..6799
                     /locus_tag="U4B62_002148"
     CDS             5438..6799
                     /locus_tag="U4B62_002148"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN2142291.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6759..7910
                     /locus_tag="U4B62_002149"
     CDS             6759..7910
                     /locus_tag="U4B62_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN2142292.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7885..9054
                     /locus_tag="U4B62_002150"
     CDS             7885..9054
                     /locus_tag="U4B62_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2142293.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9229..10608
                     /locus_tag="U4B62_002151"
     CDS             9229..10608
                     /locus_tag="U4B62_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2142294.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10889..11118
                     /locus_tag="U4B62_002152"
                     /pseudo
     CDS             10889..11118
                     /locus_tag="U4B62_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3700 a         1945 c         2493 g         2980 t
ORIGIN      
        1 attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag
       61 tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt
      121 ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat
      181 gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc
      241 taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga
      301 tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat
      361 ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt
      421 gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg
      481 ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca
      541 tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga
      601 gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca
      661 tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt
      721 gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg
      781 gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat
      841 tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga
      901 accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa
      961 aaagacacca cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc
     1021 agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac
     1081 agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc
     1141 ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg
     1201 atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa
     1261 tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt
     1321 ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg
     1381 cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata
     1441 tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg
     1501 gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc
     1561 agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta
     1621 cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc
     1681 cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg
     1741 gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt gtttttgttg
     1801 aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt
     1861 tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc
     1921 gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt
     1981 atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac
     2041 aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg
     2101 aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac
     2161 gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca
     2221 tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct
     2281 cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt
     2341 gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca
     2401 ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag
     2461 tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat
     2521 tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt
     2581 agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat
     2641 ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat
     2701 cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc
     2761 taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga
     2821 aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc
     2881 tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt
     2941 ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt
     3001 tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt
     3061 gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg
     3121 cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag
     3181 aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat
     3241 acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt
     3301 gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg
     3361 ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca
     3421 tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta
     3481 tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca
     3541 ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc
     3601 aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg
     3661 ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg
     3721 taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc
     3781 ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg
     3841 gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag
     3901 ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc
     3961 ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc
     4021 aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag
     4081 taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg
     4141 gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg
     4201 ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag
     4261 gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg
     4321 cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga
     4381 caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa
     4441 tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca
     4501 aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt
     4561 cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa
     4621 cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt
     4681 ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg
     4741 aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc
     4801 ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac
     4861 aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg
     4921 ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc
     4981 cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata
     5041 caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc
     5101 cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag
     5161 ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag
     5221 ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag
     5281 gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt
     5341 tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg
     5401 ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc
     5461 gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt
     5521 tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga
     5581 tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc atcatgttga
     5641 ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc
     5701 cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa
     5761 gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga
     5821 gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa
     5881 aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat
     5941 ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg
     6001 gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa
     6061 ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat
     6121 catcgaggaa atctctttta gagaactgag agagatggct tatatggggg ctaacgttct
     6181 tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac
     6241 caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg
     6301 actgacagga atagctggac gcaaggattt tctttccatc actttattca aacgtcatat
     6361 gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc
     6421 aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat
     6481 tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat
     6541 cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat
     6601 tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt
     6661 atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac
     6721 agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg
     6781 cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg
     6841 aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga ctactccttt
     6901 attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag
     6961 tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg
     7021 gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact aaacacattg
     7081 aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg
     7141 cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct
     7201 ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg
     7261 cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg
     7321 aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg
     7381 accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa
     7441 cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt
     7501 cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta
     7561 cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct
     7621 tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag
     7681 ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg
     7741 gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg
     7801 gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca
     7861 gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt
     7921 tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat
     7981 gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca
     8041 ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa
     8101 ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa
     8161 acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt
     8221 ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta
     8281 gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt
     8341 cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt
     8401 ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc
     8461 gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg
     8521 acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata
     8581 agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa
     8641 aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg
     8701 atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg
     8761 aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt
     8821 gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa
     8881 actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca
     8941 ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg
     9001 gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata
     9061 gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt
     9121 tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt
     9181 taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc
     9241 aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca
     9301 cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg
     9361 tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat
     9421 ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa
     9481 acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa
     9541 gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta
     9601 ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc
     9661 aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt
     9721 gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg
     9781 atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac
     9841 ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac
     9901 ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa
     9961 gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt
    10021 ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga
    10081 gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact
    10141 gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa
    10201 ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca
    10261 agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat
    10321 gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc
    10381 caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat
    10441 gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa
    10501 gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg
    10561 aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata
    10621 tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa
    10681 acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca
    10741 ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc aaaagttaga
    10801 ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact tttgaactta
    10861 aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta aaaatccaga
    10921 tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt atggctgttt
    10981 acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga aagttcagcg
    11041 cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac gtcgctacaa
    11101 ttcttatcga ggaaaagt
//
LOCUS       DASJUF010000091        10979 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.092, whole genome shotgun sequence.
ACCESSION   DASJUF010000091 DASJUF010000000
VERSION     DASJUF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.092"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(51..962)
                     /locus_tag="U4B62_002153"
     CDS             complement(51..962)
                     /locus_tag="U4B62_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2142295.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1339..1977)
                     /locus_tag="U4B62_002154"
     CDS             complement(1339..1977)
                     /locus_tag="U4B62_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142296.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1981..2751)
                     /locus_tag="U4B62_002155"
     CDS             complement(1981..2751)
                     /locus_tag="U4B62_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN2142297.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3169..3585)
                     /locus_tag="U4B62_002156"
     CDS             complement(3169..3585)
                     /locus_tag="U4B62_002156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142298.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3636..4017)
                     /locus_tag="U4B62_002157"
                     /pseudo
     CDS             complement(3636..4017)
                     /locus_tag="U4B62_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4143..4808
                     /locus_tag="U4B62_002158"
     CDS             4143..4808
                     /locus_tag="U4B62_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142299.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5205..7238)
                     /gene="brxL"
                     /locus_tag="U4B62_002159"
     CDS             complement(5205..7238)
                     /gene="brxL"
                     /locus_tag="U4B62_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HEN2142300.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7356..9878)
                     /gene="pglZ"
                     /locus_tag="U4B62_002160"
     CDS             complement(7356..9878)
                     /gene="pglZ"
                     /locus_tag="U4B62_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HEN2142301.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9994..10938)
                     /locus_tag="U4B62_002161"
     CDS             complement(9994..10938)
                     /locus_tag="U4B62_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142302.1"
                     /translation="MFKKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIFINHYSLEKE
                     LTKEVQDEYISARLPDKAREIKDFISYSIGCIFGRYSLDEDGLIFAGGEWDPSRYQKF
                     KPDTDNVLLITDDEYFKDSQIDIVNRFIDFVEITFGKETLEENLQFIADTLGGKGTSR
                     EIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQLGKVRTEYL
                     HELQKAYDNRIELRQQQISTSDNPKERNAMTKEIVKIQKQLKEVREYDEKLGHLALKR
                     IELDLDDGVIVNYDKLQRDPKTGEKFQIFAKGVKEPKK"
BASE COUNT         3474 a         2118 c         1597 g         3790 t
ORIGIN      
        1 gatgaacagt tttattgatt taaatagttc atatttttta tttataccgc ttatttacta
       61 cttgctgtac tagaatctga agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg
      121 atttttacat tagcgtcttt caatacttcg ccaattactt tattctggaa ggtactgtca
      181 cttaattttg tgtctgttgc aatttctttc aattcttttt tgtatttatc catgtcgttg
      241 cctttgtttt ggtttttcac cattttaacc acataatatt ccgtagtata agttgaagca
      301 ttggtagaag tgatcacatc agaaacttga ccatctttca acttaaaggc tgcttctttg
      361 acttctgccg gaaccgttgt agtagtggaa tcaaatttca cttttccgcc atcttctttt
      421 gtcgtagtat cagttgactt gtctttggct aatttagaga aatcgtctcc cttatcagca
      481 gctttcttca catcttttgc atcatcttca cttgctactt ggataatttg agcttccact
      541 tctggatgga aagattccca agccgttttt aaatcttcat cggtcaattt gatatgtttc
      601 tttagacctt cttgaaaggc tagactttgt ttgatactgt ctttgaatgt ttgttctgtg
      661 taacctgcag atttcaattg tgaatcaaat gaatcaccca attgtttctt tgtttgatca
      721 tattgggaat cgatttcttt ggttgatacg tcatctccat atttatcttc aaatacttga
      781 taaacgatca actgttgcac agcggaatct gctccttgca tttttttcaa gctatccatt
      841 acttcttgtt cgctgattgt accgccttta taactaacgg ctgttttttc accggcattc
      901 gtacagcctg acaataagcc agtgcctagt acgatggcaa gtgctaaaaa tctttttttc
      961 atttatcaat tctcctactt ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga
     1021 tgaaaatcat caagaacacc tttactaatc tgctcgatac gtcctgaata gatataaggc
     1081 tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc acaccttaat
     1141 atactacttc ttttgttacc gtttttcaag tctatcttgc gttccgtatt taacgtaact
     1201 aattcttaga acaaatgtta tcgattttgt ttttcttctt ccgctgttgg gatattcaaa
     1261 tcagacactt ttatgttcga ttttccagac atttttttgt tgtttttatt agaaaaagtt
     1321 tgttttttca tttgttttct aaagcatgat cttcttgttt cctattttgg tcagatatgg
     1381 atcatgcgtg accacgataa ctaacgtacc ttgggaagcc tgttgcgata agatttcaac
     1441 gacttgctcc ttatttcgtt tatctaatga tccagttggt tcatcggcta aaatgatttt
     1501 gctattcttc aataagactc tcgcacaggc gattctttgc ttctcaccac cagataatgt
     1561 gtagataggc gtacttagct tcttgtttat atttactttt ttcaatgcct catactttgc
     1621 tgttcgctta tctgaaacat ttaaattcga aaacttcaat ccaatatcta aattaaaatc
     1681 gattgtttca tattcaatca acccaaaatc ttgaaaaaga aaagagaggg catttctttt
     1741 tatcaagcgc ttctctcttc cagttaaatg ttttgtttct tgattaaata aataaaattg
     1801 ccctgaactg aattgatcat tcaatcctaa tatattcaat aaagtacttt tccctgctcc
     1861 gctctctcca actacagtca caaaatcccc agcatccgca gtgaaacgat ccaaatgaag
     1921 aatcgttttg tttcgaatga tttttttcgc accttttatc actatcaacg gctccaattg
     1981 ttaacctcca ttcatcagca ataactgact tttcttttct gtagacaaaa taaaaacaac
     2041 tgaaagaata aaatcaatga agctaaagaa aaaaatatta aaaagatatt taggctctct
     2101 ttctataaga aaaaggaaga ttacacttgt cgttccccat ttgatgaggt tcatcatgaa
     2161 gaaatcttta tacgtttcaa agagcgaata accacttgaa cgaaagatag cgattttccg
     2221 acaattgtag acaaaatata tgcataacat gacatagctc aacaagaaga aagaaaagaa
     2281 agtgagtaaa aatatctgta tcaagtaatg aaatgatctg cttaacactt tgacagctat
     2341 ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta tcacgtaact gactttcttc
     2401 aatcagctca aataaccttt ctttcgacaa agagcctatc ttgatcttta acggtggata
     2461 gatttctccg ctgagaatat ttctttccca atacgtactg tttttcaatg tcagtatttc
     2521 aacaaccgga taatgttcga tccaggggat agaaggaata aatgtataaa tgggttgatt
     2581 gttttttata aatagaaatt tcggttcttt ttcactgatt tcttgataat attctgttat
     2641 ttgcgccagg tcatttttta aatctgtatg tctatatcgt tcagggatca atataacagg
     2701 gtgcgtttct ttttcggtta tttggatcaa ccttccagag acatcaatca aaggaaattt
     2761 cttaagataa ttcagattta tttttatccc tctgccaaac tgttcatttt cttcattcaa
     2821 gtaatctgat acattgacta atatacttcc gtttttattc aaagtttcat ataattcctg
     2881 atcggctgct gatgcaaaac gattggaata aataaaatcc acgtggtttt tcccgatggt
     2941 tactggataa aatacatgat agtcctcacc agcgatttct tttggtgttt catgggaaac
     3001 agctaactgt gaagaagaaa aaacgatagt tgctaattcg acattggcaa cggtcaaaag
     3061 aagagaaaac aacaatccaa atgtaaaaag aatgtattgg atatagttga acgcttctct
     3121 taacgaagcg attttttcaa ctaataaaag agaaatcaaa gagataccct atattccgct
     3181 gaacaagaaa acgtacatct ggaacaaata aagaaattgc tccttataat acataaaaat
     3241 aaaaccacca atgattcctg caattatgaa tgtaataatg attttctttt tccccaataa
     3301 acaccacaat acttgatgta atgaaaagcc attattcaac aataaactga tttctttatt
     3361 catcaataag aaaaaggatg ataacaaaag gaataaaaga atcaatccaa tagttaaata
     3421 cactcgataa ttatatacgt ctgtacttga aataaccgaa tacgaatagc tattttcaaa
     3481 gtcttcaaaa atattagtgg cataatcagt gtgaaatgct tgattgaatc gttccacaaa
     3541 tagtaaaatg aaccgttcat aggcttcttt ttctctcgtc atcaagaaaa attcaccttc
     3601 aaaatctttt attttgatcg attcttcaat ggacgttacg cgaaaggagg tattagaata
     3661 gggtaccttc tcgtgagaag atttgacatc cccttgataa tacaatgtca tttcaagctt
     3721 tggctgtatc ttgaagttcc aagatgtatc tacttcattg gattcattta ctgttttttt
     3781 catataagaa agctcagttt ctgatgagac attttcaaat accatgatta gctgctcata
     3841 ttcttcttgc ccaataaaat gttgtggcaa tcgaatcgga gtataagtat ctgtttgatg
     3901 gaaaaattca tttgtccgat agagtacctg tttatttata ataaccaagg caaaaccact
     3961 catgataccg gtcactatga ttgtgagtaa aactgtgaac ttttttttga ttatcaccac
     4021 tctcccttac ttaaatgatt ctttgataaa atcataccgt ttttcatcaa aaaaacgtat
     4081 ctatcaaatg tataggtcat gtagaataaa tctatatact gtagaatcga aaggagaaga
     4141 agatgaacat cggagaaaaa atacgaactt accgcagaaa ttgtaagctt acgcaaaaag
     4201 aattaggcga aaaaatcggt gtttccgata aaacaatctc tagttgggaa aattctcgta
     4261 caatgccaga ccttgaaatg ctgtcacttt tacatcagac tttaggtctg ccgattgatt
     4321 ttccttctat tgaatcagct accgaatcaa tagaaccaac attgccggat gaacctcttg
     4381 ttatcaagaa atcttattca tcaaaacaac ccttctcact caaaaagaca ctatctttct
     4441 ttctgctcgt cttttgtttg gtctatcttt ttcttatcaa ttatcaatat aaaaatgaaa
     4501 cggttgatcg attcaatcct tttttgaaac cacaaatcgg ctataccaca ctccctgtag
     4561 gatacgtgga aaattccatc gaacattggg tatgtaatga tatctttgtt catggtacat
     4621 ggctgtcttt tcacgctgga gaggtagaaa aaggtaagaa tcatgctgta atcaaacaca
     4681 aaggaaaaca cgtcatgaac gttcgcctgc tcacttggga agccattccc tcagattacc
     4741 aagaaatcct catccaagac tatgacgatg ccttaaaaag aagccctcag gagccagata
     4801 ggcattaagt aggagatgaa cgtagagata gaaaaaatca atgcacatga tcttatcaaa
     4861 aaaaaacagg ggtacaaatt aattgaaaaa gctttgagat gaaagttcct aaataaaaaa
     4921 gttcagaatg ataggtaaaa aaatgactaa ctattctagt attaggctat ttgtattatt
     4981 cttctgttat ttttatatca aaaaatccag aaataactaa atataggtat ttctggctta
     5041 tagtataata tttacgccac agcccccaat gattcttttt ggaatcatga tcaaggacgg
     5101 acatacacaa aaatattttg cccattcgat ccgaatagat taacgactaa tctagtaagt
     5161 aatatagatt ggtacgtagt caaaccagca aatggaaaag ttgtctattc caccccaagg
     5221 gctttaaaga cagcatcttc cggcgtttga taaaaaatca aattaaaggt acccattaat
     5281 tctgggggta cagttccaat atcagcagcg cttatcatag gtacaagtac ttttttcgct
     5341 ccggcatctt ttgctacttg taaacagtta gctaaattat ctaccttaat catagaacca
     5401 ccaattgtaa tatcccctaa aataactaaa cttggtaata caggtttatt taaggaaact
     5461 gagcataaag ctattatagt tgataacgat aatgtttttg tcataccaat gccattcata
     5521 tcttgaacat gaacgacaaa atcttttgtc gtcaaactaa tagactgact aacacttcca
     5581 taattagctc ttaaatatcg gtatgccgaa tccatcgctt ctcttgctcc agcatctgaa
     5641 ttgatcccag tacgttcaaa ctttccattt cccgaagtca tttgcgtttc caatttatag
     5701 gtacctaatt gcccagtttt gccataagat accgtgtaca aatgccctgg cttccctaac
     5761 ccttctggaa tcaaagttcc tccaccttgt tcaggaacag aaacatactc ttctcgctct
     5821 tcttctaaat caatgtatga aaaattgaca tcataaaatt ccattccacc aattttcttt
     5881 aattgttctt ttacacgacg acgcatttca agagcaaatc ttagtatttc ttcgacatca
     5941 cttttttcaa atctaccatc aggatacaac agtttcatca tcccagatac aatccgacga
     6001 actgcaatcg tatcccgctg atttaattga gaaccaagtt tgaaatattt atcaaaacta
     6061 tcgccaaaac taacttttcg aagctcacgc atgacttcag aaaaataatc tgtaatgaag
     6121 ccatagtcat ttgtaaaaaa atctggtcga tatttaggaa tttcccaacc tggtaaataa
     6181 caatgcatcc gatccaaaaa ggctgaatcc gtccccataa cctccggaaa tggctcaaat
     6241 aaattcgaag ttttcaatat tacatcaacg ctttgattaa tatttcctac aaaagccatt
     6301 gatgctgaag cattcttttc ttctttgcct ctagcaaaag atcctgaagc catgtaatct
     6361 ttcatgattt gaacgccatc attatcctta aatttaatcc cagcaacttc gtcaaaagca
     6421 acacagtccc acatacctac aagtcccatt gtacgactag ccatattata gaaaaggttt
     6481 gctactgttg tttgtccacc tgaaacaagg attgagtttg gtgaaatttc tttatataca
     6541 tgcgatttac ctgtaccacg tggtcctagt tcacagagat taaaattatt ttcaattaat
     6601 ggaataagtc ttgctaaatg aagccatttt acccttttat taaattgtgt aggttccata
     6661 ccaatggacc gtagaagtaa gtcaatccat tcatttgtag taaactcctt gcgagccatc
     6721 ttgtaagcct caatgtctaa accaggcatt tggattggat caaccttttt tgcaataaaa
     6781 gggctattcc aacgatcatt ctcatcatac caatattcca tcgaaacaat acaccagatc
     6841 cccccaccta ataatcgatc aaattttttc ggatactctt cactaatttg aacatttttt
     6901 aatcccaggt tagagaattc tgcttcataa caatctttct tttcatttaa atgtacagtt
     6961 actttatcaa taactgtata accacccaat tcacgaattt ttgcttttac tttttctgct
     7021 tcatcaggac gcacatagtt tcgagcaaga atatttttaa cattttccac acctttttca
     7081 accaatgttt catccaatga cgaagcatac ataccaagta aatattccaa tacataaaca
     7141 ggtacattcg caccttcttt aattttcttt gttaaatctt ttctgacaat ctttccggga
     7201 aagaattcag ataactttaa aatttcctgt tgttccacta gaattcctcc atgtgttcag
     7261 tcattataat agcaattaaa gtatatcata cttaaccagt aggcttgata atacaaaaga
     7321 aaaagtatcc acaaaaaatt gtggatacct cgtttttata tatcaaaatc aaatgattct
     7381 gcaaaaggat tatctataat gaattttatt cgctcggtca aatcattttt ttgatttttt
     7441 atcactaaat aataagtatc ttcaactgta tatcgacgat ttacaaattc aaaaacaaat
     7501 ttagtaaacc gctgatttgc atcttctgtt tttgaatccg caacatagat ttcttcattt
     7561 gagatacgtt caccatattc atcttcaaaa taaagccaga aagatgcggg gtcataaaca
     7621 tcactaattg catttttttg atagaattct aacgtggttg ataatcctgt aattctgcgg
     7681 ttgctagtca ttaattcaat tgctactggt tcctttgcat tgctaccttt ggctaccgta
     7741 atttctagaa cagggactag catttcttga atagatgacc ctccatgaac gtagctttgt
     7801 cccccgccag ctttcttaaa aatacttgaa gtaatcggga cacaaatgtt tctagtgtca
     7861 tcatttccta aagaatctcc caaagagtaa tttttcgtac caattatttc gtattcttct
     7921 tcactaagaa taaaacgacg ttctacacga tctttttcat ttctagatgg attttcaatc
     7981 ttatctactt cttcaacgtg gcttctttta taaataaatc catgatcagc cgtaactata
     8041 aaacgataga tactgccatc attatgcaag cgattaataa gtcgggaaat ttcgcttatc
     8101 gctaaatcac aggcctcaaa aactcgattt tcattcgagt gatcacctgt tctatccact
     8161 tgatcgtgat aaatataaat gactgatttg ccattaaata cttctcgtac ctcagcctta
     8221 ttcatcttaa ccacatcatc ataatttaaa gctacagcat ttgcatctcg ttgctggaga
     8281 atcgcatttc gattggataa accattcgtt tttttcccat caatcagtac ttctgttcct
     8341 tcattgtaag ttagttgatt gtttggtaat aaggcggctt taccgaattc agtaactgaa
     8401 ggtaatacag aaagaaagct agccatgttt gttgaatact tcttttctct aactaactta
     8461 cgttctaaat tcatccctaa ctcatatcga aaagcatctg aaataataac tacagtttta
     8521 acagagcgtt tcattacagc ttgattataa aattttcgaa tggatggacg attcttaaat
     8581 tcaaaagttt gattccaaat ttgtcctgat tgatcaagaa acttcgaata atttgcttca
     8641 actaatcgat acaattggcc atatttttgc ttttcttcta tatgatcact attccaaata
     8701 aatttccgat aatgcgtatc aacccaatac atttccttct catattgagt tagcaaatta
     8761 tccatgttca tcggcaagca caatctgttt ttatccacaa gataataagc atgtttcaat
     8821 aattgatact cataaatata atccgatgcg aaatgcattt tttcgcgctg ctttaaacta
     8881 tcaaaaattg tttgtcctgc aattttcgtt gtagtgtcag aagcctttaa tcgttctgca
     8941 taaaacatta atatttttgc atgaatctca gaaaatacgt ctgcttcaat tagatcatca
     9001 atttctatac tgtctaataa tccatcacca tcaatagcct gatatacttt tttagatagc
     9061 tgttcaaagc tttccatgta acgtgtatca ttcataaact gatccatgaa agtaataatg
     9121 tttgctttca aatgtagtat ttcaaatcct ttgaacttct tcggcatctc ttgatcaatt
     9181 tgataataaa aatagttcaa ataaagacac atcgtcagct tttccaatgt tggctggcta
     9241 tccgtaaagc caaatttcat ttgaataaat tcccaaaatt tagctgttaa ctgatactta
     9301 tcaagttccc ttaaaggatt ctccctactt tctgtaaagc tttttagaac tacacgcagt
     9361 aaatcattta atcgaatact tttcgatttc aacaaaacag ccattatcat taattcaatc
     9421 gtttctgatt tattaattgt catatgcgta ctaaattcac caaaacttct catgcgatcc
     9481 tttgcattaa agaaattacg atactgttta actgtctgat gaaatctaag tggaatagcc
     9541 aattcattca tgataatcgc taagcggtct gcactaaatt cctctgaata attcaataca
     9601 ctcaacaaat gattatcttc atctttatca tcccaacgag gaaatggcgc atagattaaa
     9661 tagttagttt cgggatcttc atattccaat aaacgcttag ttttaaattg tgcgtccttt
     9721 tctaatcgat agatttttgc attttgaatg accaatgagt caatttcttc aagaaaatcc
     9781 tgatttgaat caaaccaaaa aatgattttc ctacatccat aattaggaaa acgttgattt
     9841 aatttttctt caatttgttg cgtatttata tctgtcatca atctcaccta atcgttctct
     9901 ttctataccc cttatattga cataatttgg ataatttcgc acgcaattta agaataaaaa
     9961 agaaagttgt tagaaaacaa ctttcttaga taactatttt tttggttctt tgaccccttt
    10021 tgcaaaaatt tggaatttct cgccggtttt aggatcacgc tgcaacttat cataattgac
    10081 aattacacca tcatctaagt ctaattcaat tcgttttaat gctaagtgac ccaacttctc
    10141 gtcatactca cgaacttctt ttaattgctt ctgaatcttt acaatttctt ttgtcattgc
    10201 atttctttct tttgggttat cacttgttga aatttgttgc tgacgcaatt cgattcgatt
    10261 gtcatacgct ttttgtagtt cgtgtaggta ttcagtacgt acttttccta attgatctgg
    10321 tgtatagcga tgcaagtaca ttaacgcttt aaagccattt tgttttcctg agtccaattg
    10381 ccaataaatt ggtcgattgg agtatgtttt taaatggtct ttgtaaaaat cttgtacgaa
    10441 gtaacgacga ataatttctc gacttgtccc tttgccacct aaagtgtcag cgataaactg
    10501 gagattttct tcaagggttt ctttaccaaa agtaatctca acgaaatcaa tgaaacgatt
    10561 gacaatgtca atttggctat ccttgaaata ctcatcatcg gtgatgagta aaacattatc
    10621 agtatcaggt ttaaattttt gatagcggga aggatcccat tcgccgccag cgaagatgag
    10681 accatcttcg tctagggagt aacgaccgaa gatacaacct attgaataag aaataaaatc
    10741 cttaatttcc ctcgccttat caggtaacct tgcagagata tattcatctt gaacctcttt
    10801 tgttaattct ttttcaagag aataatgatt aatgaatatg tcgttcagtt cctcttcatt
    10861 cgattttaga gtattaaatt gattaaccaa ctgcctactt agacagttaa atgcctctga
    10921 gagatctttt ttaaacatat aacttaacaa aatgtttctt ctaaactcgt atgacgttt
//
LOCUS       DASJUF010000092        10972 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJUF010000092 DASJUF010000000
VERSION     DASJUF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.093"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            823..1296
                     /locus_tag="U4B62_002162"
     CDS             823..1296
                     /locus_tag="U4B62_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN2142303.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1305..1532
                     /locus_tag="U4B62_002163"
     CDS             1305..1532
                     /locus_tag="U4B62_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142304.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2168..2539
                     /locus_tag="U4B62_002164"
     CDS             2168..2539
                     /locus_tag="U4B62_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142305.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2795..3037)
                     /locus_tag="U4B62_002165"
     CDS             complement(2795..3037)
                     /locus_tag="U4B62_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2142306.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3069..3971)
                     /locus_tag="U4B62_002166"
     CDS             complement(3069..3971)
                     /locus_tag="U4B62_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2142307.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3984..4172)
                     /locus_tag="U4B62_002167"
     CDS             complement(3984..4172)
                     /locus_tag="U4B62_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2142308.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4186..4749)
                     /gene="amaP"
                     /locus_tag="U4B62_002168"
     CDS             complement(4186..4749)
                     /gene="amaP"
                     /locus_tag="U4B62_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2142309.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4787..5680)
                     /locus_tag="U4B62_002169"
     CDS             complement(4787..5680)
                     /locus_tag="U4B62_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2142310.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5758..6681)
                     /locus_tag="U4B62_002170"
     CDS             complement(5758..6681)
                     /locus_tag="U4B62_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2142311.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6730..7080)
                     /locus_tag="U4B62_002171"
     CDS             complement(6730..7080)
                     /locus_tag="U4B62_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2142312.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7113..8015)
                     /locus_tag="U4B62_002172"
     CDS             complement(7113..8015)
                     /locus_tag="U4B62_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2142313.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8008..8865)
                     /locus_tag="U4B62_002173"
     CDS             complement(8008..8865)
                     /locus_tag="U4B62_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2142314.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9199..10008
                     /locus_tag="U4B62_002174"
     CDS             9199..10008
                     /locus_tag="U4B62_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2142315.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10048..10545
                     /locus_tag="U4B62_002175"
     CDS             10048..10545
                     /locus_tag="U4B62_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2142316.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3203 a         2061 c         1856 g         3852 t
ORIGIN      
        1 gtatgtccta tgatgaaaag attgtactat tcattgatca taacgattgg ctatttgatt
       61 gtatcaaatc taggaaatat ggtatttggt atcagtaaag aattcagttg gactactaca
      121 ttatgggaat ctctattctt tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa
      181 taaacatgtg ccatctatct gttggcacat gttttaggat ttatggtact ttttattcgg
      241 caatccattt tatttttcta cttcttctta atacctctaa cacttttgat ttataaacta
      301 atccaatgca tctacttgcg cttattaagc tatttcagct agatagtacc accccatgcg
      361 tcaatgcttg attttccttt tgtataacat tgtataagca ataaaggaca aataagtggt
      421 gattacacca aaccattgaa taagaaaaat tctgatacgt aaataaacat gaacttgaaa
      481 aaattaatga atcggttagc tactacgtag ttgtcttttt tctcatacag ttctgttaac
      541 ttacacttct cttcgctaga taataaaaaa tcctccaact gtattgtcca atataatccc
      601 aggcatctaa actagcatta tagaagagac acacatagaa tctaaaataa ctggttttaa
      661 atgtttctta caatcaaaaa aagctgttaa atgcactcca caccttcata ctctatcatt
      721 ttttataaac gatatatttt tattgttttt cgataaaaaa tagttgtttt acaataatta
      781 ttctgctata ttttagctat caaagataaa agagggataa aaatgaaatt taaaacattg
      841 ctattaaaat ttgtcgtttt aggtataagt gccgtattta ttttgttcgg cgtacttttt
      901 tatactcaga tcgctacaag cgaaaaagga ttcaattttg attggcagac cacattattt
      961 gtttctgtca tttactttgc ccttctccta gggatcgtta ttgcttattt cttattgaaa
     1021 ctattgaaaa aaatcgaaga taaccaagct ttttccactc aaagcttaaa catactaaaa
     1081 aaaatcgaac gaaccatttt gtttattggt ttcgcctttt ttggcttact tcctaaattt
     1141 atgaaagtag cggatttaga cgatgctcca ggtttgatgt tggtaggaat cgctgtcgta
     1201 ttcattcctt ttgcagtata cacattaaca agcgtgctgg aaaaactgct tgaacatgcg
     1261 attttactta agacagatca tgatttgaca gtgtgaggtg cgacatggca attatcgtta
     1321 atttagatgt agtattagct caaaaaaaaa tgagtgtgac tgaattgagt gagaaagtgg
     1381 gcattacgat ggcaaacatc tctattctaa aaaatggaaa agcaaaagcc atccgttttt
     1441 ccactctcga aaaaatctgc caagttttgg agtgtcaacc aggtgatttg ttgacctata
     1501 aaaatgactc gaatactata ccagaagaat aaaataaagt agaacaaaca gtaaataaaa
     1561 aataatctga atgatagatg tgtgagactt ttagaagtta tttacgtcta ttattcagat
     1621 tttttatttc gattgaatac cgttttatct ataaataaga tatgtactga ctcatacaaa
     1681 gggggccttt tcttctccat gtactatcac ttttcctgat acaactggtt tcctgttacg
     1741 ttgtagttgg atctgctttt agtataatac tttattcatt ccaccagact gatagcagtt
     1801 tgagcagtct aaataatcat gtagtattac aggccattct gtttcatacc ttgaagtgct
     1861 taaaacttca gctaatggcc cattgtgtta actcttactc cattcttttt tagtaagctc
     1921 ccatatatac tgtaaagcct tctatatatt atgtcatgtt gctttttaac atttactatg
     1981 tagttttgat aaaatgattt gaataacagt gatggacaat tcttttttat ttttaacatt
     2041 attttgataa taatgattat ttatttaaaa atacattaga taacatttgt tacgacacga
     2101 aaaagcttca caaacaaata tcgttatttt aaaatgaggg cgaactcaca caattaggag
     2161 gaatagtatg ccatttattg tttacttttt tctttcatta ctcgtgatct atattccatt
     2221 accaacattt ttcctgctcg tcaatcgtct agcagagcaa catgatacga ttgctgctct
     2281 tacgaaactt tgtttacttc tatttgtttg tctagattac tttacgagct attattggct
     2341 aaaacagaca agagtaaaaa agagatatta tttcttcctt gtctttttca atctctttga
     2401 gtttctgatc cattgctttt tacttcatca gtatcatacg atccatttgt ggatcatctg
     2461 ggttttcaaa gtggtcgtat tattttgtat gttcaccttt cctatctcaa gaaaatttag
     2521 ggaggcattg ttttattaaa gaagcttctc tgtatcaata aagacacatt taaggaaaaa
     2581 cttttaaaaa tctaattggg aatttgcccg cataaataag aggctgtgac aaaagtcttg
     2641 tcacagcctc ttattccgta taaacagtgt tcaaaagctt gcatcttcga taatatgccc
     2701 aaccaaatga aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgaaga
     2761 acagcttttt cacgacctct ttttaaaagt ctttttacag ttttcgagca aagaatgata
     2821 cgattagtac taaaatgatg gcaccaatca atgatggcac gattgccatt cctgcaatac
     2881 ttggtcccca agaacctaat aatgcttgac caagtgctga tccaactaaa cctgctacga
     2941 catttgcaat aatccccatc gaagaacctt tattcgtgat tgctcctgca attgctccga
     3001 taattcctcc gacgataagt gaccaaataa atcccatgtt tcgttcctcc taatgtttta
     3061 cttccttatt actgaacccg tggttctgta ttttctttta ctttttgtgc gccgcttcca
     3121 gctgctgatt ttactttatc tgtttgattc gaagcaaatt caccggtttt tttcgctgcg
     3181 tcagttactt tgtcttgcac cgtttcttga tctttttcaa attcttcttt tgttttgatg
     3241 tccactacgt tagcgttgac ttcgatcact tctaaatgtg tcatattttt tacttctttg
     3301 ccgattactt cttgcatttc ttcaaagatt ttttcgatat ctttgccata ttccacaatg
     3361 acatccatat caactgctac ttgtttttta ccgacttctg tttcgatccc agccgttaca
     3421 ttatctgtat tcacaagttt tcctgccaca ttagagaaga aaccaccgtt tgcagataat
     3481 aaaccgtcta cttgttcaat tgcgattccg atgatttttt gcacaacttt atcttcataa
     3541 ttcagctcac cgcgcattga atcttcctgt tcagaatcat gctttccttg gttatttgta
     3601 tgatcagaac caagttttcc ttgtccgttt gtgtggtcag aactaagttt ttcttgtgct
     3661 ttcgcatggt cagaaggatg tttttgctct tccacaggtt tagaaggatc gattgcatca
     3721 tgttgttctg gaactttaga ttctggagtt cctggatgat gaggtgcatg cttttctgca
     3781 tcatgtttct tctcttctac tccttgttga acgttaggga ctactggatc tggatgtgca
     3841 gatactggtg ctgttttgtt cttttcatcg ttcacaccat ttgattggtt tggtgtcgtt
     3901 gggtctggat gtgcagacac tggtttttgg ttgttcattt tattgttatt atttttgtta
     3961 ttgttattca tggatgtttc ctcctaagat ttaaaatagt gatctaagat tttcgtttgt
     4021 ttcagataaa aaccgatagc gacgccgatc gctgccatga tcaagatcaa taaggtcttg
     4081 aaaaagccca cggtgatcca taaaacggct aatatcaatc caagtacccc aaagatgata
     4141 gggagcttgt atgtcgaaaa tagttctttc atttttggtc cccccctatt ctactcgttt
     4201 gcggtcattg cttcgtttag ctggttcttc aaagcctgtg tatttcaccg atactttcac
     4261 tggctctttt gttcccaata tgtgttgtac ttcattttcg atttctttca tcaatgttcc
     4321 tgttttacca atgagtgctg aagttcgttt caactcccct tttacattga cggcgatctt
     4381 gtttttcgtt gctttgacat ttacttttgg tgaatggatg aattcatctt catgcaagtg
     4441 agaacgaacc attccttcaa ttgctttttt atccaacgtc aactccccat caccatgttt
     4501 cagtagaaag tttccgattg tttttggata aaacaggaaa acaagtaacg tgataattgt
     4561 caaaatagca aatgcaacgg caagccaaaa tacatattgt tgcacgtatg aattagtgat
     4621 ggaaaagaat cgaaacttct ctagctgaaa aggcaatgga taggttgcac ttaatagtat
     4681 cgcaaacaaa agcacagata gcaaaagtaa tgttccgatc actccgatcg tttttacacc
     4741 tttattcatt ttcgcacctc ctcatagggg tgaaacgatc aacttcttat aagttgatcg
     4801 cttctccacc agttactccg taaatttggg ctgtaacata acttgcttta ttcgatgcta
     4861 agaaaacata aactggtgct aattctcctg gctgacctgc acgttctagt aaactttgct
     4921 gaccaaattc agggatcgaa tcagaaggtt gtccttcgtc tagttgtaat ggtgtccaga
     4981 ttggaccagg tgctacacca tttacacgga ttcctttagg tgcaagctgt tttgctaaac
     5041 cgatcgtaaa gttagcgatt gaagcttttg ttgctgcgta atctaataaa tgttcgcttg
     5101 gattgaatgc ttgaacagaa gttgttgtta cgattgctcc tcctgctggg atatgcggaa
     5161 cagcttcttt cactaaagca aacatagcga tgatgtttat cataaatgtg tctttgactt
     5221 gctcaagtgg taactcagca atagactgac aagaaatttg ctgtcctgcg tttagtacca
     5281 tcgtatctag cccgccaagt tcactcacgg ctttatctat gatttcttta ggtgcttgtt
     5341 cgtcacgaag atcaaaaggt agtaagacaa tcttacgtcc tgcatctttc acgtattttg
     5401 ctacttcctc tgcatctttt tcttctcctg ggaagaattg aagagcgata tctgcccctt
     5461 ctctagcaaa tgcaatcgct gctgcacgac cgatccctga atctccacca gtgattagta
     5521 cacggcggtt ttctagttga ttattgcctt cataggattc ttctccacaa tcaggaacag
     5581 gaatcatatt ctgctgtaaa gctggtgtat cctgatcttg tttaggaaat ttctccgtat
     5641 ggtacaagtt tcgcgggtcg attaattttg gttctgtcat tgctgtattc ctcctagttt
     5701 ttaaattgta ttgccaaaac aaatttcttt gtttgatacc tgttttaaat agattcttta
     5761 atttgtataa tctttccggt ataaatggta acctaaaact actgctgcta tcaaagagcc
     5821 aaagactacg agtataaagc ctagagcaga acttttaccc ggtaatcctc ctgtattcat
     5881 tccccaaatc cctgcgaaca acgccggaac ggaaatgatt aatgcagctg aatctagata
     5941 tttcatgata tgatttagat tattgtccat catcccgttg aatagatcac caatactctc
     6001 taataaatct cgatagatct cgatcatttt ttctgtttgt cgctgtctca aatggacatc
     6061 atagagtagc tgttcatcgt ctaaacgttt gatgaaagtt tcagaattcc ataattgatc
     6121 cattgtttcc cgttgatctc ttaaggtatg atcgatatat acgatgtctc tttcaagatc
     6181 tgcctgttta tacagttctt cattttccgt tgttttacga gctgctttat ccaaagcgtc
     6241 aattcgcttt ttcatctctc ttagttcttt cacgtaatgg gtgtaaatat ctaataatgt
     6301 atacgtgatg atcttttcaa acgagtttaa atgatttcca tgctttgtca gcagccgatc
     6361 cacaaattcg gtttttctat cgatacaagt aatcaagaga ttatcggaaa gtacaaaaga
     6421 tattggcgtt aatttttttt cgatacgatt atctgactga tctagattaa tcactaccaa
     6481 agaaaatgga tgctctagtt tcgtctcata aaggcgctcc atccgagaaa cttcttccgg
     6541 atattcggtt cctacgaaaa tatcatcagg aagctcgaat tcttcgattg cttgctcaat
     6601 ctcttcgggc tttgcactat caagtacgag ccaattatat tttttttcat ctgtttctat
     6661 tacattcttt tcttcatcca caaagtagta tctcaatgta tacctccttt ttggactttc
     6721 tcgaaactat tagctgaaga agcagttctt taatttgata ttataatcga gtactgcgat
     6781 ttcattttct aatgaaaagc tgtcaagctg tttgttgaag tccatgttca atcgtttacc
     6841 gaaataattt tgctctaatt tagatgtata gatgtccaat aatggatgct cgattttagt
     6901 cgcatattct ccaaattccg aaacaaaagg aatgatttga aaaaagctgc cttttgcaaa
     6961 agcacctttg tcccttagaa atggatcgga atcattgata agcgcgagtg cgatttttgt
     7021 attttcccca accttttcaa ttaacgtctc tactgcattt ttagacattg taatttccat
     7081 tcctactccc cctcttcagt tgttgtcttc tattatttaa caactacatc tgtttttggt
     7141 tgaacttttg catattttgg atatttaggg aatttagcta cacaacggaa agccgtcatc
     7201 cctgcatatt tttcgtaata tttcttcgtt ggcccttcaa aatccttgct ccacggtttc
     7261 acatgtcctg aaaaatggat aatgtaagga ttgttgcgag tttcttcgta ttcacgttca
     7321 cctgctgctg ttggatgttt ttttgctttt gccatgatat agccttgtgc attccaacgc
     7381 ggatgaagtg gtaaccaacg gtcatgtaag atagcattta atgcatcttg gtcatggaat
     7441 cgtaattttt ctggattatg ttcgatgaaa tctaatactt tttgcgtaat attttcatct
     7501 aaccattttt tcacattgat cagcatcaaa cctgagttga aataacgcat tgatttagct
     7561 gaaattttca ttttttccag tcgttgatgg aacccagcgt cttcaacagc tgctacgatt
     7621 gaatcaccga agtcaagacg ccataattta gagatatctt gtaaagcgat catgtcgcaa
     7681 tccatatata agatacgttc aacttctgtg ccgcggaaca attccggaat cgcaatacga
     7741 taataagctg tttctggaat acgatcgctg attaccacat ttgtaaagaa ttctttattg
     7801 attttcaaga attccacatc tgagttcatt cgtgcgtttt tcactgagaa gcgtaaaagc
     7861 tgtttacttt caaaatcgat atcatcgtca atcacataga accgaacaaa tttagacggg
     7921 ttgcaattat ctaatactga gacaaataat gcagctaaat gtggtacgaa ttttgtgtta
     7981 caacttgcga ctactgctat ttctttttta ttcattgatc tcgcctactt tcgttagagt
     8041 actgtgtgct aattttttta agtataaatt ggtatacgga tgatctttca acgtattcca
     8101 aggtttatca tgtcctgtaa agtgaacgat cgaaggtttt tcatttcctt ctttatactg
     8161 acgttcatat ttttcatttg gtgctggatg acgttcaaaa atcagcgaag tctgcatgtt
     8221 ccattttgga tgaagttgtt cccaatcttc ataaagtaca gcattcaaag catcttggtc
     8281 gtgatagatg attcgatcgc cattttcact gaggtaatgg atagtttttt ctgtaatttc
     8341 ttttttattc cattgatcaa tgtcaatcac catcactcct gaattgaagt acaagtcatc
     8401 ggaatcgatt cctaatcttt ccaaagcttt cacttggcct ggatcgataa tagcgccgat
     8461 tgtttttcca tttaacggct catcatatag ttttacgata tcgtctagta ctaacacatc
     8521 tgaatctaag taaaggactt tcttataatc ctctttagct aataagtttg gtaaagaaat
     8581 tcttaagtaa gcggtagttg tgatatggtc actgactagg aagtcttcgt agatatcttt
     8641 ttccactgtc aggaattgga tacttgcatt acttgaatat ttatttaccg tttcttccaa
     8701 tccttcttta ctatattctg atagaccatc gtcaatcaca taaaatttta tgcgtcttgt
     8761 tttattgcaa ttttctaatg cagttgcgat catcacgctc aagtaaggag cataattttc
     8821 gtctgaagca gttaccacag gaactacgcc gtatctcttc tccatcaaaa tcactccttt
     8881 ttctaatctt tttctaacct gttctacatc tagaatattc ctcatttggt tttaaagcaa
     8941 aatatatgct ttgttttcat tgaatatttt aaaatttttt tatcacaaaa cgcatcggta
     9001 agcgctaact attcgttttt catggctttt tattcaaagg tattgaccaa aaactactat
     9061 atatagccac ctttttctta agaatgtttt ttgaaaaaat tcataatgac aaagcaagtt
     9121 agtttcattt ttttaaattc tcatttaatc ttaaggtaac aggaaaatag atatgtaaaa
     9181 agaaagtagg aagataaaat gaaactagca gcgattgatt tagacggtac cttgcttgat
     9241 cgaaatggaa agataccaga agaaaatatc caagcacttc ggaaatttga tgaaaatggc
     9301 ggaatcgtaa ccgtcgctac cggaaggaac agcatttctg ctaaagatat tttttccgaa
     9361 ttaggaatcg gtggctattt gatttcttcc aatggttcac tgattgctga aatgaaggat
     9421 ggaaaaatgg cgaaagttct cagacgttca aaaatagata gcgatacact gagaaaagcc
     9481 ttcttctatg ctcaagaagc gaaaatatcc atcattgcta gccgagaaac ccaagatgat
     9541 cagctgacat tcgatgaatc aaatctagtc aaagatgacc cttattatga acatttcaat
     9601 ttacagaacc attcttttga tcaaatttta aatatgttgg aagatccttc tcttagctat
     9661 cttaaattag cattaacaga taagagtgaa gaaaaactac agaagctaca aagagatctg
     9721 caagcagatg gtatccaatc agtattctct gatccgcatt tcttagaaat cacgcctaca
     9781 ggtatcacta aagctcattc acttgttttt ctggcggatt accttggaat cgaatcgagt
     9841 gacatcatgg cttttggcga tcaagaaaac gacattgcta tgttgaatta cagcgggttg
     9901 agtattgcga tggggaatgc acagcgacaa gtaaaagaaa ttgcaaacca agtaacactg
     9961 acgaatgaag aagctggtgt tgcttatatt ttaaaaaact tctattaaaa agaataaaat
    10021 gacgatatga atgtaggagg aaagaaaatg ttagttatta ttgatatgca aaatcatatt
    10081 cttgatccaa acagcgaatt ttatattcca gagtcagatc aattggcaga acgtattgct
    10141 caaagattag ctaaagcacg ccaaaataat gaatatattc tcttcacacg ggatatacca
    10201 gttgaactga aggacaaaga agaagaacga gaagatttgc aattgatccc tcttctttct
    10261 ccacaagaaa atgaacgagt gattaaaaag tattacttta cgattccacc tgaaacttta
    10321 gctgaaatcc aggaaacttt atttgaaagc aaagaagaac aaaaagagat cgaagtagtc
    10381 gggattgaaa caaacttgtg tgtactctct aatctgatag gcctgcagag tgcttttcct
    10441 gaagcagact tttttgttga tccaatgtta gtctccagta gaaaacacgg agaatccgct
    10501 ttagagctgt tgaaagattt caacgtctct atcgtcgaat cataaaaata tctatcttca
    10561 tttacgatat ttaacacaca ataaaaaaag cacgcagaac tgttttagtc ctacgtgctt
    10621 tttctatcaa agtagattgg cttttttatg acacagagtg aaacggcagg ctcacgacct
    10681 ctttcttaaa tggtgttcaa tcagctgacc ctcggtatta gcttaacaaa cggcaaaata
    10741 tgaggaacta tttttccgtt tgttaagcta atactcaggt caaaacgctg atttcacaac
    10801 ctcttcctta aacggtattc aaaagcttgc atctgcagta ataagaacag ccaagtaaaa
    10861 acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca gctttttcac
    10921 aacctcttat gtaccttgta ggactcgaac ctacgaccgg acggttatga gc
//
LOCUS       DASJUF010000093        10752 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJUF010000093 DASJUF010000000
VERSION     DASJUF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.094"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(33..1076)
                     /locus_tag="U4B62_002176"
     CDS             complement(33..1076)
                     /locus_tag="U4B62_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2142317.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="U4B62_002177"
     CDS             complement(1237..1920)
                     /locus_tag="U4B62_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEN2142318.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="U4B62_002178"
     CDS             2115..3044
                     /locus_tag="U4B62_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2142319.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="U4B62_002179"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="U4B62_002179"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEN2142320.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="U4B62_002180"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="U4B62_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN2142321.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="U4B62_002181"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="U4B62_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN2142322.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="U4B62_002182"
     CDS             5429..6232
                     /locus_tag="U4B62_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2142323.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="U4B62_002183"
     CDS             6316..7863
                     /locus_tag="U4B62_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEN2142324.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="U4B62_002184"
     CDS             complement(8160..8357)
                     /locus_tag="U4B62_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142325.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="U4B62_002185"
     CDS             complement(8433..8633)
                     /locus_tag="U4B62_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2142326.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="U4B62_002186"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="U4B62_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..>10752)
                     /locus_tag="U4B62_002187"
     CDS             complement(9985..>10752)
                     /locus_tag="U4B62_002187"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2142327.1"
                     /translation="KPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISV
                     SLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAADITYI
                     PTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDL
                     GSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEAN
                     RALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT         3113 a         2108 c         2177 g         3354 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt tt
//
LOCUS       DASJUF010000094        10411 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJUF010000094 DASJUF010000000
VERSION     DASJUF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.095"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            606..812
                     /locus_tag="U4B62_002188"
     CDS             606..812
                     /locus_tag="U4B62_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN2142328.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1014..2015)
                     /locus_tag="U4B62_002189"
     CDS             complement(1014..2015)
                     /locus_tag="U4B62_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2142329.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2020..3933)
                     /locus_tag="U4B62_002190"
     CDS             complement(2020..3933)
                     /locus_tag="U4B62_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2142330.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(4101..4607)
                     /locus_tag="U4B62_002191"
     CDS             complement(4101..4607)
                     /locus_tag="U4B62_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2142331.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4767..5207
                     /locus_tag="U4B62_002192"
     CDS             4767..5207
                     /locus_tag="U4B62_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2142332.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5233..6390)
                     /locus_tag="U4B62_002193"
     CDS             complement(5233..6390)
                     /locus_tag="U4B62_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142333.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6393..6749)
                     /locus_tag="U4B62_002194"
     CDS             complement(6393..6749)
                     /locus_tag="U4B62_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142334.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7047..8021)
                     /gene="sgrA"
                     /locus_tag="U4B62_002195"
     CDS             complement(7047..8021)
                     /gene="sgrA"
                     /locus_tag="U4B62_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN2142335.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8217..9056)
                     /locus_tag="U4B62_002196"
     CDS             complement(8217..9056)
                     /locus_tag="U4B62_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2142336.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9241..10128
                     /locus_tag="U4B62_002197"
     CDS             9241..10128
                     /locus_tag="U4B62_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2142337.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         3131 a         2055 c         1573 g         3652 t
ORIGIN      
        1 agccactacc aaatatttgg tagtggcttt tttggttctg caagggtggt acagaacatc
       61 aatagtcggt gggttagaac gtatgtcatc gcatctttgg agaaggttgt attggtatta
      121 ttcgagttac ttgttagatg cgataacaaa gttagtatac agtagtaaaa aatttatctt
      181 ttcttttttt tattattttt gtttcttatt ttctaaaaaa ttaaaaaaaa ttttggtgaa
      241 ttaagtattt ccttgataac actggctttt ggcctcattt taggttatca tttgtaattt
      301 tatttcacag ttgatatgat tgttatgtaa aataaaaaag tttaggatac atcgaatttt
      361 tggggaaatt cttttggggg tatcctaaat cttttctatt atattcccat attttctatt
      421 attggtctag acgtatgatg gtaaactttg tacctatctg attgctgaat cattaattgc
      481 ttatcattag ccatctttgt ttcaagttcg ctagatgatc attttattca catcctaaca
      541 aacttgtcac atttatagat tcttaatcta tttatcatac aattataaaa aaggaggtgc
      601 tgcttatgaa agctatccat atttctttgc tttttttcgc tagtgcattg attttatttg
      661 ctattcatct ttgttcccag ccttatatca ataacaacgg aatattagtg gaaccagcat
      721 tcttttgctt accactcggc tttttgtccc ttcttgcctc cgcaggcttc ggatttgttg
      781 ctttttttaa acgatcaaaa caacaaatct gacttataaa atttacgtaa aagagatcgt
      841 gacaataatc gtgtgatatt cccaaatttc gaataaaagg tgccccttgc catctctaac
      901 tcctcagtca caagttacaa tattcataaa ccatgaaaag cttgtttatt gaaattgctc
      961 cttgtgactt ggagctaagt gacagattac aacctctttt gcatcataag actttactcc
     1021 ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt gtttattgct gaaatcaaac
     1081 tgatattcat ggttcaaaac agcatttttc tccgcataat ctacttttgt gactttcacc
     1141 cccgctttct caaatacaga agttacttct tccatttgtt tcgtaaacgt atttgtattt
     1201 ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat gtctgaccaa ggctaaactt
     1261 ttgaccttct ctgattgttc aaagtcctta ttatcaaagt aagcccatcc acatttttca
     1321 taaagaaagc tagagatttt atcacttact tgagtaagtg cagaaggatc caacagacca
     1381 gacaaagaaa taaacttcgt aatctgcttg ttctccaaag tcggatcaag atgatcagct
     1441 tttttaattt cttcattttg aagtgctaac aaatattctc cactaatttg tgcacctgcc
     1501 gaatttccac caatcgaaat attcgaccaa tctgcttgat cttttatctc ttttttagct
     1561 gcttgaatgg cttcgttcaa ttggataact ggtgtaggat gttttgcttc cggtgatcgt
     1621 tcataatcta tatttacgat gatttgctgc gtgtctgaac atagatattc caaataatca
     1681 cgacaatcat ttttatcccc tcctacatat gcgcctccat gcatccagaa aagaatcggt
     1741 aattttttct tttccacttt cggtgtgtag atatccatat tattattttt gtactttgaa
     1801 ggataggata tttctttttt tatatcgatt tgttcgattt tttgctggaa atcatctggt
     1861 cgggcatata cttgttcttt ttcacttgtg tcaaattgtt ttctaacaag caagctaatc
     1921 ggctttggtg tgatttgatt aatcaaaaat acagtgacta aaataactac gatgataact
     1981 ggtactgcaa tcactattcg cttgaaccat ttcattcatt taccctctct ttcttcgcca
     2041 aatccatgtg ataatcgtgc taacagataa aaatccacct gaataaatga gaagcgaatg
     2101 tttcttttct ccagttttcg gcaacattct tttttttgca tctgtctttt tttgtacttt
     2161 cactgtcatc tgaatcggtt gcgtatcatc acttgattga tcctctcgag gttcattctt
     2221 tcccttattg tctgcattct ctgtcggatc ttgctttttt ggtggagcta ctgggatttt
     2281 tttgtaatag aaaataatag ttggtatcgt ctcattaaaa atcccttcag tcggtccttc
     2341 aacatttgct aattcgtatc cttcgaatgt cttttgctct gtcatataat tttctccata
     2401 agctccgtaa agctggattt cgtcctggtc tttgagtaga cttttattgt tttcatccac
     2461 atactttacg tttatctgat catggaacac tactttcaaa aagtccgtta catgtttcgt
     2521 gtaggcagca tggtcaactt cctgtgcctt tccatgccct gctccttcta ctaataaaag
     2581 atctttatac cctcctgata actcatataa tttttgcgcg ttagacacag gtactacatc
     2641 atctgctgtt ccatggataa tcaatttagg caatgaatct gacattccaa tcgtaggtag
     2701 agcagctttt gtatccaaat gcatctcctg atcaaaatag tattgtttca aaaggcccat
     2761 aaataaatct tcatgctctt gatccccaat gatgtctaac tgatttttta tggcatccgg
     2821 tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt tgttcatcaa ttgatgcaaa
     2881 tccgcaattt tcaataattc ctttgacatt gacaggaata tctttcgcta atacagacat
     2941 catcgtagct gctcccatcg aaccaccata aagtaaaatc ttttgagatg gccgctctcg
     3001 aacttcttga tttatccagt aaaggacgtc atctgactca tattgtccat aggtcacata
     3061 atcgcctccg ctattaccag tcgctctgga atcaacaaat aataaattat acccggcctt
     3121 ataaaaatca ttgtattctt ctgtaacgat cccgttattc caattccctc gaaatccgcc
     3181 atggatcatg actgttttat ctgaaccttg atcgacataa tatgcatata catccatcat
     3241 tttgccatct tgtttgttta gtacagtttg atgaagttgt tttgtatttg gatagaagcc
     3301 tgtgttttct gtaggcgttg tcccatcatc tgtacctcca agcatcgaca tcaacacact
     3361 gttttttgca aaagcgatat cgtacaatag ataagtagca atcagatgtt tgataatctc
     3421 tttatctaca ccataatcat taacagcaag ctcagtgact aataactcta ttaattttga
     3481 cttgatgtct tctgttggtc cattaagtat ttctgtcatc tgtttaccga agtaattcaa
     3541 atattcttta ccattgaaat tttcaccttt accgatatca tcattttctg tcccattctc
     3601 tttgtcactt actttagcag aagaggattc gtcgtttgac atttttgtag ctgttgcttc
     3661 ttctgtttgt tggttatctg tggtagcggt cgtttcttga tctgtgctag taacagatgt
     3721 ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta ctcgatttcg tttcatttcc
     3781 agcatcagta gtttctgtgg tttctacctc ccgagattcc gattcggaag aaatgctctc
     3841 tccatatact gtccattgcg ggacgcttgt acttacagca aataagatga ccaacgctcc
     3901 ctttaatact tgttttgttc tcaagctatt cactcctttt tatgtttcta ggagaattat
     3961 atgtaaacgc aaacattttt tatacaaaaa ataaatggtt tatcccataa aaatagaaca
     4021 ggattatttt ctcattttac gtaataaagc agaatggata aaccagtatc cattctgctt
     4081 tttttaaacg aagaaacaac ttattgctcc aaaaatgtca gaaatcgttt atcccaaatt
     4141 ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag gattatcata agttgtcgtt
     4201 gctcctctac acatcataag ctggtatcct aatccatgag caaacttcac agtcgtatct
     4261 atacaatact ctgcttgtgc cccacataat tcgatcgaat gacagtccaa ttcagttaat
     4321 aaaacactca aattagtctt ataaaatgaa ttcgcatggg tttttctcac aaaataactg
     4381 tgctcaggaa tagataattc cgacaatagc tcccatggtt ttgtttgtgg cactaattga
     4441 gcatcttcgt gttgaacgaa aataactggt ttcccaatcg tttcgtatgt tttgattctt
     4501 ttattcacta atgctgtcac tgtttctaat tcgtgtatcg gcgaagaatc aaaacatacc
     4561 cctttttgta aatcgatcac tactattgcg tcggctgtgt ttgacatact tgtgccttct
     4621 ttcttttttc ttgatttaat agtatttctt atctagctag ttccattata cgtgacaaca
     4681 ttttcttatt ttacacctct aaatggcact tttcgactat ttcttcatgt tattatttca
     4741 taaatactta gctattggag gaatgtatga tgattaaatt gtatacaggt tctggctgct
     4801 cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga tataccgttt atcgaacaga
     4861 acttaattcg tcaaccacta accaaaaatg aatttatcca cttactaaaa ttgacaaaca
     4921 acggcgtgac cgaaatgctc tctactcatt ctattagtta taaaaatctt gttcacagaa
     4981 tagatgaact cagtttgagt gaactatata ttgagattcg tacatcccct tctttattga
     5041 aaaaaccgat tatctacgac caatctgcaa aacttttgac tggatttagc gaagataaaa
     5101 tcaggatgtt cctttctaaa actaaaagaa agcaagagct caacgaactc ttgctttctg
     5161 gtcatcttgt cgattttaaa gaaaataatc tttttgcaat gtcttaattt tggcataaac
     5221 aatctgcttt tcttaattaa cgaaatccaa tttttccaat atggatctat ttttctcatt
     5281 cctgattttt atgatttcag ctttcagacg cgcccaatca ttttttgtag gttgtccatt
     5341 ccagtaaaat atctgtgtat tcgtttgatc atatgccttt ttgtcgatag gataatcgct
     5401 aatgacaata tcaggtaact gatcagcgga tgcactatat tttattgggc gttcagtcat
     5461 agataatatc cttttctttg cccataattc ctgtgtttta ccatactttg attgaatata
     5521 gaatatgaca ggttgttcgt gttttataaa aatatccctg actaacatac agtattgaaa
     5581 aatcaaccgt ctgttctttt cataataagt attaagctct ggaattctgc gagctacccc
     5641 actaaaaaac tgttgcatga ttggatacat cactggaaat atatgctgaa aatctctgtc
     5701 ttctgctgat tttccaaaag catccacctt cttttttgtc ccaaaaaccc ttgaatacaa
     5761 atggatcatt gccaaattga catatagata atttttttct gaagttgtaa aacaaatctt
     5821 gaagtttttt tcaaaatttt ctacaataga cgattcaatc cagccccaac tttcttgtac
     5881 tttatctgga gtatccaaca aatcttcttc caaataggtg ttcattacac caaacataaa
     5941 ataaaggtaa gcttgctctg ttggagtgat gtgattcatc gaccagaagt ttgcttcaaa
     6001 aaaaggctct agtatgtaat gcgtaaactc ttcttttgtt atcaatggat gcacaaattt
     6061 gttcatctct tctgggacat acgtaatcga gtggccttgt ttcattcgtt gcaacgaaat
     6121 tcttagtagg ctgatccatt tctttctatc tatactacgc gtatatggcg catgtttcgc
     6181 aaatttttct aatagttgac gtttattttt tatacttgat aatatatcag aatgattttc
     6241 gtcgaatgac tgccaataca tcaagtgaaa aaagtatcgg atacggtatt cttctccttc
     6301 tatcggatta ccttttttca agctgcaaga taaatgatag tttttcaaaa tagtattaag
     6361 ttctcttaat tttctttgaa tcgtacgcaa acttatataa ttttttgact aagattttct
     6421 aaggattgat aagtaccttt gtaaagtgaa tccagtaaaa tataaccaat cgttccctct
     6481 aaaagtatag aataaacttt actcagtaaa aactgagaag aaaactcgac ttttatcatc
     6541 ttgttttctg aaagaaaaga taatttatat tctggatacc tttcgcaaaa cgattctgct
     6601 tgttgaatca ccgaattcat cttagccagg gtgtagttta gatctaaagc gatatccttc
     6661 accacatata atttctcgtc tctttcataa caatagttca aaaattgata tacttgcctt
     6721 tcattgatat caataaattc ttcaaacaaa gtacatactc ctttttatct attatttaga
     6781 cttattccga cagctacatg aataacgtac aaatagattc gttactgatt tttcagagat
     6841 actaccatgc gcagtgtgca ttgtcaatta ttcttgctct tattttccat ttgtgttcct
     6901 gttatttcct gtttctcaaa tatgttagta aacaaaaata gatctggcaa agctacaact
     6961 tcgccaggca atacagcact ctcgaatgaa atattcactc aaaaatctat cgttttattc
     7021 aattttagat ctgttgcttt tattctttat ttcactcgat ggaagagaga acgaaaatag
     7081 cttgctccta caatcaaaat gagtaacaca cccatccatg taattagctt gttcaaggtt
     7141 ctactaccag tttctggtaa atcgcttttt ctattatctg tttctttcgc tttttcttga
     7201 tgatcaattc cacgagaaac aaaatcaggc tgttttagca cactttttag gttgctccct
     7261 gcatttctct tttgttcctt agttggtatg ataggtacca aatcaagttc attatttact
     7321 tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac tctcactact catgcttcct
     7381 gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgct tcctgtgcta
     7441 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgttttctgt gctactttca
     7501 ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt ctgtactgct ttcactactt
     7561 gtgctatccg tactgcttgg cggtccaaca actgtccaac tgcttggttt caaattctcc
     7621 gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt actcttgatc
     7681 tcatcaatcg atagttctgc agaaatcatt attgtatact caataccagc ggtccattgt
     7741 acgtctttta aataaacttc cccaataact ttttctgaac ttccattacc atcccaacca
     7801 tatgttagaa cagcatgtac atcactagca ttcacgcttt tgtttggttt gactggaata
     7861 gtaatgactt gttgtgtcat gtcataaaca atccgatcag ttttcacagt aaatgaaaga
     7921 ttctcatttg cccgttcatt tgcagaagta ctgattccaa tcattgctgc aattatactc
     7981 agcccaagaa taacaaataa tacggtcgct gttttcttca tttctatcac tctccatata
     8041 attaccttct atttgataaa ggaattatat aaaatgaaaa tgatgagaac acgacagtca
     8101 gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt
     8161 aatcaatgac agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat
     8221 tgataaacag cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca
     8281 acaatcaaga ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc
     8341 ccagtaatta ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt
     8401 ccgctgactg gtatacccaa aatagctcct tgtgtccaat tccaagtaat atgagcacca
     8461 atagccgtaa ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa
     8521 aagatattga aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat
     8581 aatgcagcag atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa
     8641 aagccacgga ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt
     8701 atcagcggca caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat
     8761 ggactagtaa taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc
     8821 cagtcttttc ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta
     8881 gcaagaaata aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca
     8941 atccattcac tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa
     9001 acaaaaccaa ctaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt
     9061 catccctcct ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt
     9121 aaatgttctc catcatagag agaatagatt tttacgaact aagaatatct acagcaacct
     9181 tacgaaaatg aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg
     9241 atgggaaatt ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta
     9301 ctattctcag gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt
     9361 acagttcagg atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa
     9421 aaaatgattg tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa
     9481 gtaacaaaag aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta
     9541 aaagcagttg gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc
     9601 caatacggtt tgaaagccaa tgtaaaatta acagataaag atctagacac tgcttggaaa
     9661 gaattctatc cagaagttag tacacagatc attctatttt cgactaaaga agaagcagat
     9721 aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca agcttgttca agcttatgga
     9781 aaaaataaaa cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag
     9841 attcctactg aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc
     9901 attcctgtaa cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa
     9961 aaacaggata aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc
    10021 gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa
    10081 gacaatgtaa ctattaaaga tccttatgta aaaaatatat tcaaataaac aaatttgacc
    10141 caattgggga tgtggcaaaa tttgagaagt gatttttgga aattccttct tatttttgag
    10201 gcaaaccact tttgccacaa cttcatcggg gccttttttt atttgtacca aaacaaatca
    10261 ctggatttgt tacaatcatg aggatataat ttaaaaaagt tttttatttc tatcacatga
    10321 atcaatgcgc cattaagtag attctttcat gcttccctat tttttcactt gccgtatcgc
    10381 ttcaaaaaca actatcataa actcaaactc g
//
LOCUS       DASJUF010000095        10361 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.096, whole genome shotgun sequence.
ACCESSION   DASJUF010000095 DASJUF010000000
VERSION     DASJUF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.096"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(78..320)
                     /gene="acpP"
                     /locus_tag="U4B62_002198"
     CDS             complement(78..320)
                     /gene="acpP"
                     /locus_tag="U4B62_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2142338.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(442..1443)
                     /gene="plsX"
                     /locus_tag="U4B62_002199"
     CDS             complement(442..1443)
                     /gene="plsX"
                     /locus_tag="U4B62_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN2142339.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1492..3528)
                     /gene="recG"
                     /locus_tag="U4B62_002200"
     CDS             complement(1492..3528)
                     /gene="recG"
                     /locus_tag="U4B62_002200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN2142340.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3645..5327)
                     /locus_tag="U4B62_002201"
     CDS             complement(3645..5327)
                     /locus_tag="U4B62_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN2142341.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5379..5741)
                     /locus_tag="U4B62_002202"
     CDS             complement(5379..5741)
                     /locus_tag="U4B62_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2142342.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6011..6199
                     /locus_tag="U4B62_002203"
     CDS             6011..6199
                     /locus_tag="U4B62_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN2142343.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6437..9106
                     /locus_tag="U4B62_002204"
     CDS             6437..9106
                     /locus_tag="U4B62_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN2142344.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9561..10307)
                     /gene="truA"
                     /locus_tag="U4B62_002205"
     CDS             complement(9561..10307)
                     /gene="truA"
                     /locus_tag="U4B62_002205"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2142345.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2825 a         2233 c         1863 g         3440 t
ORIGIN      
        1 tacgtgtcaa aaagattaaa aaaagaagga taaaaagctg catgcagctt tttatccttc
       61 tttgctttta tttgaaatta tttaagatgg ctgctgatat aatctactgc gccaccgact
      121 gtttcaattt gttcagcgtc ttcatctgaa atttccgtac cgaattcatc ttcaagctct
      181 aaaacgaatt ccataatact aattgaatca gcttttaaat catctttgat gttcaattca
      241 tccgtcactt tatcggtatc tatctcaaaa tgatttgaaa taattttcgc tactttgtta
      301 aatacttctt cgcgagtcaa ctttttcacc tccaagaatc atacacttgt caattaccta
      361 caccatggct aattttactg gcatttttca ggaatgtcca cctttttttc aatggaacgg
      421 atcgcttcag atatccaaat actattcttc gcttttttca aattgttcga ctaattgtcc
      481 aactacatct gtctcaagca ttgtgtggat ttggcggatc gtatagcgaa cagcatcggg
      541 gcctgttgct ccatgagttt taatgactgg tgctttcaag ccaaacaaaa cggcaccgcc
      601 atgtttggaa taatccattt ctgctttcaa agaacgtaag ccgtctttta aaagcatagc
      661 gcccattttt cctttgatcc cttcatttag aatagcagat tttaatagat tcatcatatt
      721 cattgctgtt ccttcaatag atttcaaaac agcgttgcct gtaaatccat ctgtgaccac
      781 tacatcagcc acaccattga gtagctcacg agcttctacg tttccaataa aattcaagct
      841 tgtttcattc tgaaggagtt cataggcttt tttcgtcact tcactacctt ttgttgcttc
      901 tgttccgttg tttaagagtg cgacacgagg acgtccgata cctcggactt tacgtgcata
      961 aaaagaacct aaaataccgt attgcaacaa atgttctggt ttgttttctg cgttagcgcc
     1021 aagatccagc atatcgaaac cggcaccttc tttccctact actggcaagg tagacatcaa
     1081 gccagggcgt tcgatatttt tgatacgtcc tacgatgaac aaccctgctg ctagtagcgc
     1141 gcctgtattc cctgctgaaa atacagcatc tgcttcaccg tttttcactg cttgtgcagc
     1201 aaggaccatt gatgcagtct tttttcgtcg aattgctttt actggctcat catcactatt
     1261 gattttttcg tctgtatgga taatcgtgat gtttttttca tttgttaagt atttcttgat
     1321 ttctgcttct tttccgtaaa gtaagaattc tacttctgga aaatcttgct gcgctaaagc
     1381 cacgccttct acaattgctt gaggtgcgtg atctccgccc attgcatcta cggcaatttt
     1441 cattgtttcc actccttttt ccgtcatcag tataaagtat agcacagtct gctaatcgaa
     1501 ataacgatta tcctgatttt ctatttggag gctttgcgct aaaggctgat actcaggaag
     1561 catctgccaa tcctttattt gccaaagggc tgcagcctct tctctggcta cctccaaaac
     1621 atttccatcc gtcaccaagt cagcaatcgc aaattgaggc agaccagatt gacggaagcc
     1681 aaatacttca ccaggaccac gtaattccag atctttttcg cttagtacaa aaccattggt
     1741 ggtctctgtc atgattttca tccgttccac acctagttca tttttaggat tagcaactaa
     1801 gatacagtag gaagcatcac ttccgcgtcc aacacgtcca cgaagttggt gcagctgtgc
     1861 caatccaaat cgatccgcat ccatgatcag catgacggtc gcgttcggca cgtttacccc
     1921 tacttcaatt acagtagtcg aaactaatat ctgcatctgg ttgtctttga aggcttccat
     1981 gattacttct ttttcctgat tcttcatttt accatgtaac aagccaactt catattttgg
     2041 tgcaaatagt tcacgaagtt tttcatagat ttccactgca ttttttacat ctaatgcttc
     2101 tgattcttca atcagcggac agatgacata catttgatgt cctcttgcca gttctttttt
     2161 cgtccaatcc agtacactgt ccaattgagg tgttcttacc cagcgtgttt cgatcgggat
     2221 acgtccagct ggaagttcat caatgataga tacatccatc tcaccatatg ctgtaatagc
     2281 taatgttcta ggaatcggag tcgctgtcat aaataagaca tctggtcgcc agcctttttc
     2341 acgaagtact ttccgctggt tcacgccaaa acggtgctgc tcatccgtaa taactagacc
     2401 taaattctga aattcgactc catcttggat caatgcatgt gtgccgatca cgacgttaat
     2461 ttccccttgc tctagttttt caaggagctc acgtcgttct tttgctttcg ttgaccccgt
     2521 aatcaatgcg atggttactt cattgggatc aaacagctgt tgcaagcttt ccatatgttg
     2581 ttgtgccaag atttctgtag gcaccatcaa cgcaccttgg aaacctgctg taacggtagc
     2641 gtataacgca atcgcggcaa ccactgtttt tccgctacct acatctccct gcaaaagtcg
     2701 ctgcatatgc tgcggactgc gaagatcccg acagatttca ttcgttacac gtttttgtgc
     2761 atttgtcagt tcaaaaggta gcttttgagt aaattgtttc aatttttgga catcataaag
     2821 gatctccaaa ccattcgttt ctgctttttc tgcttttttc aatccttgaa gacgcatttg
     2881 aaataaaaag aactcttcaa aaacgacacg tcgtttggct tgatggtttt cttcgtggtc
     2941 ttttggaaaa tgcatacttc ttacagcaga agctcggtca agcagtcgat atttctccac
     3001 aaggttgttt ggcagatttt cttcaatcaa tgtgccgtat tcttgaaacc cttttttgat
     3061 tagatcaact aatgtcgttt gacgaattga tttgttgacg tgatatatcg gcgaaaagtc
     3121 atccattgtt tgagaaccta aaatcttcat cccgttcaag gctttacgct ttgcatccca
     3181 tttaccgtat acggcgattt cctccgataa aatgactttt tcttttagat aaggttgatt
     3241 aaaaaaagaa acgttgaaca catcatgttc ttgcatcata cgaaattgca atcggctttt
     3301 tttataacca aatcgattca tcacaggagg cgagactacg attcctttca aagtaacctt
     3361 ttcttgatcc tggatctcgt ttagatttct ttcctgaata tcttcgtagc gaaaaggata
     3421 ataagtcagt aagtcttcaa tcgtttggat gcccaagctg gcaagactag ctgctctttt
     3481 ttctccgact cctgccagca tagtgattgg atcatctaaa gaacgcataa atttttcctc
     3541 cctcctttaa caaaaaaagg acttggtcag cgatcatacc tgatcactaa ccgaaggcct
     3601 tttatgaatg aatactgtgt tagaaagata ttctgacaca gaaattattc agcagaaaat
     3661 agataaggat agacaggctg atctccttgg tggatctcga tttccaaatc aggatgtttt
     3721 tctgttagtt tctcagcaaa ttcatttgct tgttcaactg ttccttcatc acctgccaag
     3781 atcgttacga tctctgtgtc ctcattgatc atttgttcta gtgtacttaa cgctgttgct
     3841 gtgatatctg ggttagagat caggattttt ccatcgatca tgcccaaaaa gtctccttct
     3901 ttgatctcta caccgtcaat tgaggtatct ctgatcgcgt gagtgacttg accgctcaca
     3961 acgctagaca acatttctgt catcgtttct ttgttttctt ctaaagaagc ttgatcattg
     4021 aatgaaagca tagctgtcaa tccttgggaa atcgttcttg aaggaacaac tgctactggg
     4081 atatctgcta cttctgctgc ttgatcagct gccatgaaga tgttcttatt gtttggcaag
     4141 acaatcactt tatctgcgtt tacttcttga atcgctttaa caatgtcttc cgtacttgga
     4201 ttcattgttt gtccaccact gataacataa ttagcgccta aactttcaaa cagctcttgg
     4261 acgcctttac cagcagcgat cgctacgata ccatacggga tacgtggtat ttcttttttc
     4321 gcaaactcgt tcacttcttc atcgtgttcc aaaatcgtct catgctgcaa gcgcatgttg
     4381 tcgactttga ttttgactaa tgaaccgaat ttttgtccat aattcaagac ttctcctggg
     4441 ttttctgtat gcacgtggac tttgatgatc tcgtcatcat tgaccacaag taaagaatct
     4501 cctataccat ctaaatagtt acggaattct tcgtaatcga atgtactatc gactgtaggt
     4561 ccttctccga tttttaccat gatttctgta cagtaaccaa aatggatatc ttctgtcgcc
     4621 atttgacctt ggacactgcg atgatgttct gcattgacca tatcatccat ttctgctgga
     4681 gaaggttcat atgtttcatc cgcttggaat tctccattca atgcacttaa aaagccctca
     4741 taaacaaaca gcagtccttg ccctccactg tctacgacac cgacttcttt taggactgga
     4801 agcaagtctg gagttttagc taaagcacgc tttgctcctt ttacaacagc tgtcattact
     4861 tcaacgacat catcagtact ttttgctttc ttttctccag ctttcgctgc ttctctagct
     4921 actgtcaaaa tcgttccttc tactggtttc attactgctt tatacgcagt ttcaactcca
     4981 tgagtgaagg ctgcagcaag atcagaagca tttaatgaga caacatctgg aatttgctta
     5041 gaaaatccac gaaacaattg agacaaaata acgccggaat ttccgcgcgc acccatcagc
     5101 aatccttttg aaagcattgc tgctaattct cctacttttt ctgaggttga atctactact
     5161 gccttagctc cactagtcat agatagattc atatttgttc ccgtatcgcc atctggcaca
     5221 gggaatacat ttaatgaatt gacgtattct gcatttactt gcagacgatt cgcacctgcc
     5281 tggaccattt cctggaactg gcttgcactt atttctgtta cactcaccta gataatcctc
     5341 cttcagatcg actgctgagt taccagccag tctcctgcct agtctggcag tacacgaaca
     5401 ccttgtacaa agacattaac tgaattggca gttacgccta ataatgcctc tagattgtat
     5461 ttcacttttt cttggacgtt gcgggaaact tcagagattt tcgtaccata gctgataatc
     5521 gtataaacat ctacagcaat accattatct tcttggcgga caactactcc tcttgagtaa
     5581 ttttctttac gcaaaatctc agtaatatta tctttaattt gatttttgct ggccattcct
     5641 acaacaccga acacatctgt cgcggcgcca ccaacaactg ttgcaattac atcattggta
     5701 atttcaatgg ttccagctga cgtttgaatt tttacagcca tgaatgagcc tccttaacgg
     5761 gtaaaaaact cccatttttc ttctatcata ttttatcata gcgcaacttg aaataaaagg
     5821 cagttaccca aagtactcaa aaaccttgca aaatttcagg tataaccttg tttaatttac
     5881 aattattctt ctcaattgtc aattgtatct atgaaaatgt acttgcaaat tcccattcta
     5941 tatgatatgt tagtctggta tgagctgaat tattctattg ctccaaatca aggcaaagga
     6001 ggaaattacc atggcaaaag tttgttactt tacaggtcgc aagacaaaaa gtggaaacaa
     6061 ccgttcacac gctatgaact caacaaaacg tactgttaaa cctaacttac aaaaagttcg
     6121 cgttatggtc gacggaaaac ctaaaaaagt ttgggtgtca actcgtgctt tgaaatcagg
     6181 aaaagttgaa cgcgtttaat tgaatgatac ttggaccgtt cagatagtga acggtttttt
     6241 atttaataaa aatttttaag ggtgggactt ttgattccta cccttttttt gcgtttattt
     6301 ttgttatacg aacaaattat gaagttattt attctaaatt ttattttttt gtgaaaacta
     6361 gactcttttt aagcagagaa aaacaaggat atgataaact gaatacaact tcatagaaat
     6421 ttggtggaaa ataaatatga aaaaaaattt aaagtcattc tttagagaaa acggacgcta
     6481 cgcagcagct agtttcctga ttccgttttt gattttagca cttgtttatc taagtatcgg
     6541 aatctatcct ggaagcagca gaagtatttt ggcaagtgat gccttttcac agttttctaa
     6601 ttttcatgcc agcttccgaa atgttcttct tggaaagcaa agtatttttt atacatggaa
     6661 tgcttctcta ggattgaatt atttatcttt aatttcttac tatctaggcg ggatcttcac
     6721 gccactggtg ttcttttttc caaaccagat gatgccagat gcactgtatg ttctgacttt
     6781 gcttaaggtc ggctgtgccg gattggcttt ttggtttctt gcacgtacgt tcaagattcc
     6841 tcgctggggg catgttgcac ttagcgtcag ttacgcattg atctcattta tcactgcaca
     6901 ttcggagatc atcatgtggc tggatgcatt tttttatctt ccattagtga ttttggggat
     6961 tcatcgtgtt atggatctta aacggccgaa acttttattt atcagttatt tactcttgtt
     7021 tttatccagt ttttatatgg gttttatgat tggcgtattt tctttcttat attttatcgc
     7081 ccgtctattg acaaactggt cagaatataa aaaaacgatt gttccttatg gtatcacttc
     7141 tttactggca ggcggcgctt caatgatcat tgtattgcca gcagtgctgg atcttcggtc
     7201 aaatggcgag gcactgagtc agatcactac gttgaaaaca gaagcgactt cttatctgga
     7261 tctaatcatg aaaaatatga ttggtgttta tgacacgaca aaatacggat cgattccgtt
     7321 cgtttacgca ggtctgctac cattagtcct ctgtctgttt tattttgcca gtcggaaagt
     7381 accgttgaaa aataaatttt tgtacggcag tcttttcgcc atacttatcg caagttttta
     7441 tcttgttcca ttgaatttgt tctggcatgg tatgcatgca ccgaatatgt ttttattccg
     7501 ttatgcctat ctgttctctt tcttagtgct cctgcttgca ggaaaaggat gggaaaagat
     7561 agaacaagac gatcgtgggc tgttgttagg aatcattttg gtcgtagctg ctctctttgc
     7621 ggtggcatgg ggcatcaaac ctgaaggaag ctacacctat gtgacgatga cttcctttgt
     7681 attgacagtg gttttcttag ggatttatgc ttttgctgtg ggattttatc agcataaaaa
     7741 actttcattc aatcatctga gtatattgtt attattgatc atgtctgctg aagcactggt
     7801 caatacgaac agtatgatcc atggcattct tgatgattgg aattatgctt cccgcagttt
     7861 atatactgat ccttatcctg acttgaaagc tcttgtaaat gaaacaaaaa aagattccga
     7921 ctcgttttac cgcttggaaa atctggatcc agtatctgca aacgattcga tcaactatgg
     7981 ttacagtggt atcagtttgt tttcttctat aagaaatcgt aattcttcct cttacctgga
     8041 taaattaggt ttccgttcaa gagggacaaa tttgaatatc cgttataaca ataatacttt
     8101 gctgatggat gggtttacag gaatcaaata taacatttct aaaaacgaaa gcacgttcag
     8161 taaatatggc ttccgccaag ctggtcaaag cggcgactat acactgtatc agaatgtcaa
     8221 tgcattgcca ctagcattta ctgcaccgtt gtccgttaat gatgttgagc agccagtgac
     8281 agataatttg accagtcaga caaacttgat caatacattg tctggtctaa atgaacagta
     8341 ttttacattc tatacaccta ctttgaagaa tcagttgaat acgacgatca caaagacaac
     8401 gactggcgtc acttactcag aaacagcaga taaccagcca aaagagctca cttgggaagt
     8461 ccatgtgcct gcaaatacac aagcttactt aagtttgttc ccaaccaatt ttgcgcaatt
     8521 agaaagttct agtgcgacga tcactgtcaa tggtacatcg cgaaaaacgc aaatcaacat
     8581 ttctggtcaa tactatgatc ttggctatta tcctcaagag acaacagtga catttactgc
     8641 aagtttttat gggacgaaag aaataagttt catggagcct aaagtcgttg gtctagatgt
     8701 tgtagcctat caagccgcaa tgaatcagat caaagaaaaa ggtgtcgaaa tggaagccac
     8761 tggcagaaaa gcttctggga ctgtaaatac cgctgaagat tccatgttgg tgacaactat
     8821 tccttatgac aaaggatgga aagctaaaat tgatgggaaa ccagtcaagg tcagcaattt
     8881 caaagacgca ttcttgatga taaaagttcc agcaggtaaa catactgtca ctttcagtta
     8941 cttgcctgaa ggatttacga taggtgcaat cctttttgtc ctatgtattg cattgtttat
     9001 tctttatgtc tggtaccttg accgcaatcg ccccttttat ggcacgattg atccagcaat
     9061 agaagaacaa ccacgaaaca cacacagaag aagacgtaga aaataaagaa acgatggaac
     9121 ctgttcagac tgtccgcacc aagggcaatc aaaacaacgt tccatcgttt tttggttaca
     9181 gaaaaaatag gtaacaaaaa gatgattcac ttatcaacgc ctcgtccaca agtcacgata
     9241 ttcatcaaag atgagaagct gttttttgaa attgctcttt gtgactcaga gttaaatgac
     9301 aggctcacga cctctttctt aaacggtgtt caatcagctg accttcggta ttagctcaac
     9361 aaacggcaaa atatgaggag ctattttccc gtttgtttct ctaatactca ggtcaaaacg
     9421 ctgatttcac gacctcttcc ttaaacggtg ttccagaagt agcttcttcg aaaataagcc
     9481 gaaattttgc aaaattttga gaagtaattt tcgtaaatac cttcttattt cttgaagctg
     9541 accacttcta tcataacctc ttattcatat ttcacttcat acaaataaag tccttcaggg
     9601 tgtgctgtag gtcctgctgc attacgatct tttttcgcaa taatatctgg gatactatct
     9661 tcaggcaatc ggccattgcc aattttcaaa agcgttccta ctaagatccg tatcatctta
     9721 tacaagaagc catttcccct aaatgtaaac aacaattcgt cttttgtttc atttacctcc
     9781 atcttagcct cgtagatcgt acgtatttta tcctctatcg agcttcctga tgcacaaaaa
     9841 gaggtgaaat cgtgagtccc taataaatca ggaagcgccc gctgaatttt ttccaaatca
     9901 agtgggtaag gaaaataact tgcataatgg cgtcgaaaag gactgcgtgg tttccctata
     9961 tccactcgaa attgatacgt cttttctttg accaaatatc ttgcgtggaa gtcttctgaa
    10021 acaatttcta cttgtcttac agcaatatcc tctggtgact gggtatctag cgcgaaacgc
    10081 attcgttcta acggacgctc ctctggataa tcaaaatgga ttacttgccc gatcgcatgt
    10141 actccggcat ctgtccttcc cgaaccaaag accgtgattt cttttccatt tgccattttt
    10201 tgtaatgttt tttctacttc ttcttgaaca gtacgcccat ttggctgttt ttggaatcca
    10261 ttaaaatttg ttccgtcgta cgcaatgatt gctttgtaac gtaccatgtt tttctctcct
    10321 ttgttcctaa aaggttatct ggaataatga tttttctata a
//
LOCUS       DASJUF010000096        10343 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJUF010000096 DASJUF010000000
VERSION     DASJUF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.097"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            229..378
                     /locus_tag="U4B62_002206"
     CDS             229..378
                     /locus_tag="U4B62_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN2142346.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            389..1903
                     /gene="dltA"
                     /locus_tag="U4B62_002207"
     CDS             389..1903
                     /gene="dltA"
                     /locus_tag="U4B62_002207"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN2142347.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1900..3105
                     /gene="dltB"
                     /locus_tag="U4B62_002208"
     CDS             1900..3105
                     /gene="dltB"
                     /locus_tag="U4B62_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN2142348.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3150..3383
                     /gene="dltC"
                     /locus_tag="U4B62_002209"
     CDS             3150..3383
                     /gene="dltC"
                     /locus_tag="U4B62_002209"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN2142349.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3386..4654
                     /gene="dltD"
                     /locus_tag="U4B62_002210"
     CDS             3386..4654
                     /gene="dltD"
                     /locus_tag="U4B62_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN2142350.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4795..5877
                     /locus_tag="U4B62_002211"
     CDS             4795..5877
                     /locus_tag="U4B62_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN2142351.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5877..6680
                     /locus_tag="U4B62_002212"
     CDS             5877..6680
                     /locus_tag="U4B62_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2142352.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6750..7490
                     /locus_tag="U4B62_002213"
     CDS             6750..7490
                     /locus_tag="U4B62_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2142353.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7501..8505
                     /locus_tag="U4B62_002214"
     CDS             7501..8505
                     /locus_tag="U4B62_002214"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN2142354.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8550..10247)
                     /locus_tag="U4B62_002215"
     CDS             complement(8550..10247)
                     /locus_tag="U4B62_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142355.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKFYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3320 a         1707 c         2093 g         3223 t
ORIGIN      
        1 tttggaataa gaggttgtga cacgattttt gtcacaacct ctttattttt tatatttttt
       61 aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc
      121 tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt
      181 gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg
      241 cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt
      301 gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt
      361 atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga
      421 tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt
      481 tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg
      541 cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg
      601 ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat
      661 cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga
      721 agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc
      781 agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac
      841 ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt
      901 tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc
      961 gccattttct tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat
     1021 gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc
     1081 agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga
     1141 acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga
     1201 attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa
     1261 tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt
     1321 actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat
     1381 tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc
     1441 aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca
     1501 aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt
     1561 attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg
     1621 ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa
     1681 ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa
     1741 acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga
     1801 ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa
     1861 aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc
     1921 ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta
     1981 tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt
     2041 ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt
     2101 ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca
     2161 ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact
     2221 cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc
     2281 tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca
     2341 tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc
     2401 tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt
     2461 ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat
     2521 tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt
     2581 tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct
     2641 ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat
     2701 ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg
     2761 ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa
     2821 aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta
     2881 atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt
     2941 ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct
     3001 tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc
     3061 ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat
     3121 taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag
     3181 aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt
     3241 tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc
     3301 aagtccctgt atctgaattc gagcgagagg attggtgcac accgaataaa atcattcaac
     3361 aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt
     3421 tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc
     3481 taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga
     3541 tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga
     3601 attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta
     3661 tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg
     3721 ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt
     3781 cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata
     3841 cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt
     3901 attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa
     3961 aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct
     4021 gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga
     4081 tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa
     4141 tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac
     4201 gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg
     4261 tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa
     4321 cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc
     4381 tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt
     4441 caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat
     4501 ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga
     4561 aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca
     4621 acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt
     4681 gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa
     4741 aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat
     4801 acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa
     4861 gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat
     4921 gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt
     4981 tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg
     5041 gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc
     5101 aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta
     5161 acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca
     5221 tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat
     5281 attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt
     5341 atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat
     5401 gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt
     5461 acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc aggagacact
     5521 ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt
     5581 tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag
     5641 gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa
     5701 atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg
     5761 cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca
     5821 gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga
     5881 ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat
     5941 ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact
     6001 tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc
     6061 tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag
     6121 ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt
     6181 ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt
     6241 taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca
     6301 gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt
     6361 tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat
     6421 tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga
     6481 aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt
     6541 ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg
     6601 tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc
     6661 cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg
     6721 taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag
     6781 cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt
     6841 tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg
     6901 atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat
     6961 tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg
     7021 gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac
     7081 atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa
     7141 caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat
     7201 tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg
     7261 atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca
     7321 cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac
     7381 acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca
     7441 aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac
     7501 atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat
     7561 ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca
     7621 atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa
     7681 aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga
     7741 aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa
     7801 gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa
     7861 ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc
     7921 aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc
     7981 aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct
     8041 cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa
     8101 tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg
     8161 tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga
     8221 aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt
     8281 tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa
     8341 atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga
     8401 cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat
     8461 tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg
     8521 gaatccacat atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt
     8581 tgaacagtga tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga
     8641 tccgctacgt ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg
     8701 atgcctcgac taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa
     8761 tgttcctctt gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca
     8821 tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg
     8881 ttgataggaa gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca
     8941 gctaacttct ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac
     9001 agcatttcga aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt
     9061 tggtccgtat gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt
     9121 gtatttactt cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt
     9181 ttgattttta agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt
     9241 agattgtagc tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc
     9301 gttgtttgat agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc
     9361 aagacgatcc aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg
     9421 catgcaagtg aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat
     9481 tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt
     9541 tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag
     9601 cagatgatga aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat
     9661 ggttcaaaaa gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa
     9721 gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag
     9781 atcgtataga aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc
     9841 ggcactgatt gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaaatttc
     9901 ggtggcagtc cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg
     9961 atataatttg ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga
    10021 taatctttcc gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt
    10081 cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat
    10141 gtttcgtctt tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca
    10201 ggatcatctt ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc
    10261 gcaggagaaa aaataaaata aaagaaataa gcaattcctg ttaaaaataa caatgataat
    10321 ataatgatta tgctccactt tat
//
LOCUS       DASJUF010000097         9949 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJUF010000097 DASJUF010000000
VERSION     DASJUF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.098"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1556)
                     /locus_tag="U4B62_002216"
     CDS             complement(45..1556)
                     /locus_tag="U4B62_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2142356.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1878..3230)
                     /locus_tag="U4B62_002217"
     CDS             complement(1878..3230)
                     /locus_tag="U4B62_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN2142357.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3383..4459)
                     /locus_tag="U4B62_002218"
     CDS             complement(3383..4459)
                     /locus_tag="U4B62_002218"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN2142358.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4655..6400
                     /locus_tag="U4B62_002219"
     CDS             4655..6400
                     /locus_tag="U4B62_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN2142359.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6680..7117)
                     /locus_tag="U4B62_002220"
     CDS             complement(6680..7117)
                     /locus_tag="U4B62_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN2142360.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7101..8474)
                     /locus_tag="U4B62_002221"
     CDS             complement(7101..8474)
                     /locus_tag="U4B62_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142361.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8796..9284)
                     /locus_tag="U4B62_002222"
     CDS             complement(8796..9284)
                     /locus_tag="U4B62_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2142362.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9306..9908)
                     /locus_tag="U4B62_002223"
     CDS             complement(9306..9908)
                     /locus_tag="U4B62_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2142363.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2912 a         2119 c         1798 g         3120 t
ORIGIN      
        1 aaaatgaaag tttctcttaa aaaagagaaa ctttcatttt atgtttaatc gttatttgaa
       61 cggattacaa atccgcgttt tttatcattg tgacgtttac cgccatcttt ttggctgtta
      121 ttatttttgc tataaccgcc ttttgatttg tttccacgat aattaccgcc gtcacgacga
      181 ttacggttgt ttccgccacc acgtttgcca tttttattgt atcctttttt ctgcattggc
      241 aatgggcgtt ctggtgtgat cttcactggc actgcatcag ctggatcttt tgctgtcgtt
      301 tttagcaaaa gtgcaactaa atcttgtgca gaatattctt ctaataattt atctgctgtt
      361 tgcaggtatt tatctagacc attttctgca agttttgttt cgatttgttc aactgcagca
      421 cctaattgac ctttgaatgc ttctttttcg gttggtggac gtaaagtcgt catccgtttt
      481 ttcgtcaagt tttcaatcac atgcaagtag tccatttcat ttggtgtaac gaaagtcaca
      541 gacataccgc cttttccggc acggcctgta cgtccgatac ggtgaacgta gctttctgga
      601 tcttgaggga tatcatagtt gtacacatga gtgacacctg aaatatctaa tccacgtgca
      661 gcaacgtctg tcgctacaag gatatctaaa tgaccgctct taaatgaacg caatacgctc
      721 attcgttttt gttgagacaa atcgccatgg attccttctg cacggtaacc gcgtgcttct
      781 aatccgcgtg ccaattcgtc tacacggcgt tttgtacgtc cgaagacgat tgttaattca
      841 ggtgtttgaa catcaaacaa gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg
      901 acatagtatt gatcgatcaa gtcagctgtc atttctttcg ctttgatctt cacatgatgt
      961 ggatttttca tgaatttaac gccaatattc ttgatagctg gcggcatcgt tgctgagaaa
     1021 agtaatgttt gtctttgatc aggaacttgt gaaatgattt tttcgatatc ttccaagaag
     1081 cccatattca acatttcatc tgcttcatcc aaaactaatg tttgaacggt tccaagtttc
     1141 aatgtgtggc ggttaatgtg gtcaagcata cgtcctggtg tacccacaac gatatgtgga
     1201 cgatctttca agccacggat ctgacggcca atatctgctc cgccgtaaac ggcttgtaca
     1261 cgtacttttt tgtcgcgccc taaacgatat aattcttctt gtgtttggat agctaattca
     1321 cgtgttggtg cgataactaa accttgtaat tcgtgacgat ctggatcgat tttttctaac
     1381 attggtaatc caaatgcagc tgttttccct gtacctgtct gtgcttgtcc aataacgtct
     1441 tttccagcta atgctagtgg aatcgtttct gcttggatag gcgttgcttc ttcaaaacca
     1501 gcgcgttcga ctgatttcaa caattctggt gataattcta gttctttaaa tttcaaatga
     1561 atcctcctat atggttgtta actgaggatg tagctttttt gctaccccgt cgatttgttc
     1621 ggtcaaagtg tattgtttct ttttgacagt ggccacgcac gtttttatcc attcttagcc
     1681 cctgtggcac caccgaaaaa tcactttgcg aagtttaggc attagaaaag ccaaacgggt
     1741 cgcgttcagc tttatcatta ttatgacatc tggcttcaac aagttgtttc tatggcaaaa
     1801 ccagtccaat ttctcttcat ctttcggcaa taggttattc taacatagtt tttcgttttc
     1861 tgcaagttta cagattttta tttcatttgc tgaagttgtt ctaccacttc aaataagccc
     1921 atgctgttgc tgccttttag cataatacta tctgttgctt caagattttg tttgatagca
     1981 tcaatcaatt tattcttttc tttttcaaaa tagaacacag gtagttgggg gtatttttgg
     2041 ttcaattctt cttttagtgc atgcatttgt tccccataaa ggaaaatcat ggcgaatttc
     2101 tcaggatcga tatgttcact catttgcgca tgcatattta aagactcttt ccctaattcc
     2161 agcatatctg ctaacacagc gattctgcgt ccttttgttg gcagattttt aaaagtatct
     2221 aataccagtc ccatggctgt tggatttgca ttgtagacat cgctcaaaat agctgcacca
     2281 tttcctgccg ttagccattc ggttctgttt tgagtgatag aaacatgggc gagcccttgg
     2341 cgtatctcag gtatggacag tccaaagaag cgcccgatcg tatacgcaat caaagcattc
     2401 gttacattat aacttccagg cagcgggatc gtaaatgttt ctccttctac tacaaaatcg
     2461 gtttgtgctt ttccttttgc tgtcacttcc gcatgaacat cgccatttcc aataccgaat
     2521 gttgtgactg tttgtactaa tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc
     2581 acaacgagta atccgtctga tacaagtccg tcagtgattt ccattttggc tttagcgatt
     2641 ccttctcgcg acccaagatt ttctatgtgt gcttcaccga tcattgtgat tgctgcagct
     2701 tttggttgag ccataagtga aagttccgtg atttcattgg cgtgatccat gcccatttcc
     2761 aaaacgatct tttctgtatc ttggggcatg tgcaaaatcg tgtaaggaag accaatatta
     2821 ttgttgtaat ttccttgcgt cttatatgtt ttgtattttt cagacaagac agacgcggtt
     2881 agatctttcg tggtggtttt accattgctt cctgtaattg caacgacttc tgcgcctact
     2941 ttttccaagt aataggttgc caattgctgc atggctttca aaggatctgc tacaggaata
     3001 actggaaaat tttttggagc ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg
     3061 tttgtgatcg cttgctggat aaattcatgt ccatctcgcg taccagcaag aggaacgaat
     3121 aaacttcctt cggttatctt ccggctgtca aattcgacat tggtaatctt tgtcagtgca
     3181 gagcattcgc tgcccacggc ttgtgcaatt tcttgtattg ttagattcat tgagatctct
     3241 cctaaaaaag gttgtgaaaa aagcgtttgg cctaaacggc gggttcgcat gactctcact
     3301 tccaagtaaa tttctctatt cgtatttaga agctagaatg aacgcggtta cgcaatcact
     3361 ccagcctctt ctttatttct ctttattcat ttttttcaaa aaaagattga cgctgatggt
     3421 atcgattcat tcctaactgg atcagttctt caatcaaatc accgtatttc aagcccatat
     3481 tttcccataa gagtgggtac atactgaact ccgtaaatcc tggcatagag tttaattcat
     3541 tcaggaataa ttcattttta tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac
     3601 ctaacatcgt gtaagctaac ttcgcgtact cttgcgcttt ttgataaact tcttctggca
     3661 cttcggctgg aatctgcatt tcgattttat tattgatata ttttgcttca taatcataga
     3721 atgctacgtc ttttacgact tcaccaggca aagtcgtccg aacgtcttca tttcctaata
     3781 cagcaacttc gatttcgcgc gcttcaattc cttgttcaac gattgctcga gaatcatact
     3841 gataggctgt tgctaaagca ttttgcagct cttctcggtt ttctgctttt gtaatgccga
     3901 cactagaacc catattcgcc ggtttgacaa acatcggata aagcaaagaa ccttcacatt
     3961 gatcaaatac ttttttagga ttttctttcc attgattctt aagtactggt acataaggaa
     4021 cttgcggcac accagcagct tgtaaaatat acttggtcat gattttatcc atggcacatg
     4081 cactggtcaa tacgcctgcg ccgacataag gcatattcaa tgtctctaag aagccttgga
     4141 tcgttccatc ttccccgttt ggcccatgta aaactggaaa aacgatggct ccttcttctt
     4201 tgatttcgcc cggattgatc acttttcctg taaagccttc ctctgtctgt ccacttgggt
     4261 cccatgaaag atgcaagaca tctttgctgg caggtttttc tgttagtaat ggacctttga
     4321 cccattgtcc ttcttttgta ataaatacga gttgaacttg gtaataatta taataaatgg
     4381 catttaaaac tgaaaatgcg gaaagaatgg acacttcatg ctctgcgctg cgtccgccat
     4441 atagtaaagt aatcttcaaa agattgtcct cctgtattct aaatcgttca gtgatttact
     4501 ctcatatttt agcacaaaac aaaaataaac gaataactta tttataaaaa aacgagccag
     4561 cttctaaaag aacgagcgtc atttattttt tgtcagctag attatttgtc ccgtttttta
     4621 taaaaagtta tgataaagaa aaggagggat gatgatggcg caaacaaatc cacgcagccg
     4681 cttggcagaa atcattcgag tgttcatcaa ttacaatgta gtgccaaact ttgtacagca
     4741 aaaaaatccc gaacaggtaa aaaaagcctt tgaagaacta ggccccacct tcatcaagat
     4801 aggacagatg ctttctgttc gagaagattt gctttcttct gccttcactc aaacattcaa
     4861 aacattacaa gatagtgtac ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac
     4921 agaactttct ctttcattat cagatatatt cgatgatttt tcaaaatcgc cttttgcctc
     4981 tgcttctatg gggcaggctc atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa
     5041 gatccaacac cctaacatcg ccgaagagat ccgacttgat cttcaactat ttgaacgagc
     5101 gatccctttg atcaaataca ttcctgaaac tagcgtagtt gatctaaaag gggtactcca
     5161 agaagtaaaa cgttccttga tcaatgaaat ggacttctta aaagaaagtc aaaatggcga
     5221 acaattctat caaaagaaca atggctggaa agagatccgc agccccaaga tttatgacgc
     5281 tttttgttcg aaaaaagtga tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt
     5341 aatgaacgcc gaaaacaaaa ctgaaacttt tatcactgga attcaaaaca agcagctgaa
     5401 acaagaagtt gcaaagcttt tagttgagaa ctttatgaaa caggtgtttg atgatggttt
     5461 tttccatgct gatcctcatc cagggaatct gctattccat gtactaacaa aagaagaaca
     5521 gacacaagct agccgaaaaa ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt
     5581 tcgagcctcc acttctgcag aagaccctgt tgcgccatat accatcaact acatcgattt
     5641 tggtatgatg gggcatcttt ctgcaggact taggcaaaaa ctcacacaag ccgttttagc
     5701 gctgtatacg aaagatgctt atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga
     5761 aggctcgttt gatgaaagtc ggttccacca agaactgaca agttttcttg aacagtatta
     5821 tgattctcct attgatgaaa tcaacttgca agaagtattc acacatgtcg tgacgatttg
     5881 tcatcaaaat aatctgcagt tcgatcgtga tatcacgctt ttgctgaaag catttgggac
     5941 attagaaggg gtgatccgcg tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc
     6001 ttttgctcaa cattattttc tcactcatct ggatgtagaa gatacattga aacaaagcgg
     6061 attagactta ttggaaggga tgaaggctgc gcctaagatc cctcaacagc tccaccatct
     6121 attagaaatg tggacttcgg gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa
     6181 attattgagc aggatcgaaa gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc
     6241 attgatcgta gggtcctccc tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt
     6301 ttccttatta gggatattta cgtatgctat tgccgcattc gtcattattt ttttggcaat
     6361 cgatgcattg atccaaatgt ataagaagag gaaaaagtag aaaaaggttg tgacagaaag
     6421 aatcagcttc aagaaataag aaggggtttt acataaattg tttctcaaat ttttgtgaaa
     6481 attcggctta tttttcgaag aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa
     6541 gctgttctgc acccagtaat aagaacaatc aaaaagggaa catggaacac atttatcctg
     6601 tttccctgtt ccctttttga tgtattcgag cataaataaa ataccttcgt tacctactat
     6661 cttctgtact tggtaaacct tagtcaattg ccttttttaa cgcttgacga aaagacatat
     6721 gatctttctc cgtaaagggt tttggaccct ttgtccttat gccagctttt cttaactttc
     6781 cactatgata acgttgttct agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag
     6841 agtgatggaa cactcgcaca cccttcggca atacaagtga agctaaacca taatcttgcg
     6901 tgatcaaaaa gtcattcttc ttaaccatac cgacgatttt ataatccgca ctatcagctc
     6961 cgcggtctac gtagacgatt tggacatgat tagcgtattc tttggtcgta taatggtcca
     7021 cactggtcac gataaccacg tcaagaccat aggattctcc taattttatg acatcttctt
     7081 ttactggaga accatctcca tcaatgaata gcctcattct ttttcgcctt cttccataaa
     7141 tgataaacag ttactaatcc gctcagtaaa agaccaacat tgatccattt tgccaagata
     7201 agcagttgta cactttctat cggcactgat tcatggttca gcgtgatcaa taatagatgg
     7261 attggctctc ccatttcttt tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg
     7321 atcacactcc ctttattttg agtagtatac ttgacaacga tccggcgatc cattggaata
     7381 gctgtatcag tttgataata ttccttattt agccagctgc ctgttactac gtgccagaaa
     7441 ctaagcagaa agacacctat cgcaaaacca tttggaatca agaggaccaa taaagaagaa
     7501 ctcagtccaa gcaattgcat agccaatgga gaccatgtta aacgtttcaa aatccatggt
     7561 ccgactattt ttgctgcaat caagcctgct gcataaggca aatacataaa aatcgaaagt
     7621 ttttctaatc cgtacagtat gccaacataa aaactgctaa ataaaaaaag gaaattccct
     7681 agcattccat ttagaaaagt aaacagattt agccagcttg gcaacttcca ttccttcttc
     7741 tggtgtccta aaacaaacac gaacaagatg aacagcagca agaaaccata aatagcaaga
     7801 tcaaaaagcc tttcatcgaa taacaatcgg gcatttctca aaagaaacaa taagccaaag
     7861 aatatcaaaa ataaaacaag ctctcgcgta gctaccagat ttcttttgat gtctttgaaa
     7921 tccagttcgt aacgcggata atgactaacc gtatggtagg ccatgacgta aaaaagtgta
     7981 taaatagtaa agcttgctgc aatctgagtc ggtgcaggca actcaatcgc ttgatacagg
     8041 agaaatagcc agatagctgc aaataagtac tttttcccat ccatattgat aaaaccttgc
     8101 gtcttttcgt gaaaattgac cgtggtatta gcaggtacaa gccatgctgc actcagccct
     8161 agcaaaacac ttgaaaaata aaataaagga aaataaaata cgcctaatat tccaagcaat
     8221 gaaccagcac caccaatcag cagagcgatc atcagcaacg tgtaactatc tagaccaaat
     8281 cgtagactgt tgactaaaaa tagtccagtc atccgaaagg cataaaacag cgtaaatggc
     8341 aaaatcgtca gccaattatg cgagtctgtt agtcccaaaa ataaaagata aggaataaaa
     8401 ataaccgtgt tacttaagat aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg
     8461 agtttttcga acatgacaat ggctccttaa acagtcttat aactaatctg ctcttcgatt
     8521 ttagcattaa aggggaaata aaccaatttt acagaatata ttcttgtaaa aaaaaataca
     8581 gaaagaagca taagcttctt tctgcatttt tacggtactt gattactcaa gcagacaagc
     8641 ccttctccac aaaagtcttg cccgacaccc ctgcatccgg caggtgataa agatagaaat
     8701 caattatcct gtgcctccaa aatcaattag gagctctgta aaccagagtc cttctcgacc
     8761 cgtcgcataa atttacttta acacagttcc tatgtttatg cgagagcttt tttcatttta
     8821 taatcgacca tttgaaatcc ttgaccgata tctgaaataa gttcttctgt aatctcaaat
     8881 ccttcatgct tataaacctc gattgcttga tgattcccct gattcactcg caaaaagatc
     8941 tctctttcct ttgtttgcgc agcaagttct tcataccagc gaaaaactga agaagtcaat
     9001 ccttgaccac gtactttatt tagcaaatat aatttactta aataaatttt tccatttatc
     9061 tcttcataag cagtgtaacc gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca
     9121 ccttctgtca gttctttttg aatctgtttt tttgactgat aagttttcag catgtaatcc
     9181 acttgttttt gaccaatgat cggcacaaaa acttccggcc atacttcttg tatgactgcc
     9241 atcagctcat ccagttcatt ttctgttact tttatttttt ccatacaccc acgtcctttt
     9301 ttacattata aatgaccttg cgcttgatgg aataaaaaga gcatgatcat gctatacaca
     9361 cccaccgacc acggtttcaa gaaaatcaca atctgcataa attccctaaa agataatttc
     9421 gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa agggaagcag caaggtaaca
     9481 actagtactt ttttaatatc tttcgttttt cttgtcagca attcatcaaa tttctgatac
     9541 ttttttggcg ataaaataag ctggacaaag gctacgccat agtaacggac aagcaaaaat
     9601 ccgattgctt ccccaatcac taggccaata caactatata aaagcccaaa tccattgcca
     9661 aacatcagca tgcccgctac tgaggagatc cctcccggca aaataggaat gatcacttgg
     9721 atcacttgca acagaacaaa tatcatcact gcatacttgc caaattgctg gataaacgct
     9781 tgcaaagacg tgctcgatcg aaaaatccca tgatgataag cataaagaat caacacaaag
     9841 aaaaaaaaca gaccaataat gggcatgatc tgaagacatt tttgtaaaac tgatcctttt
     9901 ttagacatct atattcccca catctttttt catttattaa aactgcctc
//
LOCUS       DASJUF010000098         9831 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJUF010000098 DASJUF010000000
VERSION     DASJUF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.099"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..879
                     /locus_tag="U4B62_002224"
     CDS             10..879
                     /locus_tag="U4B62_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2142364.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVF
                     LSYVSIDDLSNLI"
     gene            1356..1556
                     /locus_tag="U4B62_002225"
                     /pseudo
     CDS             1356..1556
                     /locus_tag="U4B62_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2268..2873)
                     /locus_tag="U4B62_002226"
     CDS             complement(2268..2873)
                     /locus_tag="U4B62_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2142365.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            3055..3390
                     /locus_tag="U4B62_002227"
                     /pseudo
     CDS             3055..3390
                     /locus_tag="U4B62_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3555..3782
                     /locus_tag="U4B62_002228"
                     /pseudo
     CDS             3555..3782
                     /locus_tag="U4B62_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3908..4879
                     /locus_tag="U4B62_002229"
     CDS             3908..4879
                     /locus_tag="U4B62_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2142366.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            5019..6332
                     /locus_tag="U4B62_002230"
     CDS             5019..6332
                     /locus_tag="U4B62_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2142367.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            6375..7241
                     /locus_tag="U4B62_002231"
     CDS             6375..7241
                     /locus_tag="U4B62_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2142368.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            7238..8086
                     /locus_tag="U4B62_002232"
     CDS             7238..8086
                     /locus_tag="U4B62_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2142369.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            8100..9572
                     /locus_tag="U4B62_002233"
     CDS             8100..9572
                     /locus_tag="U4B62_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN2142370.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9589..>9831
                     /locus_tag="U4B62_002234"
     CDS             9589..>9831
                     /locus_tag="U4B62_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2142371.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         3131 a         1666 c         2000 g         3034 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata atattcctag aaaatcactg gattatcgaa cacctttgga agtatttttg
      841 agttacgtaa gtattgatga tctgtctaac ttaatttgac aattaagaca tttaaaaaga
      901 aaagtgacag ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt
      961 ctaatactag gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt
     1021 gtcagctatt ctatattaac attgattgct atggttttag gtacttcatt ttataatgct
     1081 tttgtaagta agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca
     1141 agtaattaat atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca
     1201 cattaagaac gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc
     1261 atatttgttt gttatgctat acatagatct gcataattag acttgcgctg cccgttattg
     1321 aagtttcagg cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga
     1381 aacgctggca gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct
     1441 accccactgt tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag
     1501 atatagatac ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag
     1561 aaacaagcga agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca
     1621 actctaagct cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa
     1681 tacatggatg tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag
     1741 ttgagtattg cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag
     1801 atagctccat ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc
     1861 tgtataatag aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg
     1921 ttcactatcg aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt
     1981 taactgtggg aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg
     2041 gggtgactga actaaccgct gatatgagcg atacttggct gtaggaatat ctttttccgg
     2101 aaaaacaaac aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac
     2161 taatgaaaaa gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat
     2221 agaaacgaaa atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc
     2281 gctttcttta ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca
     2341 actggaactc cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga
     2401 tctgttgagt tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc
     2461 aataatactc cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa
     2521 cttagcaatg ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta
     2581 aaatctaaaa actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta
     2641 actgtatttt ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca
     2701 caatcacctt ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta
     2761 ccactggctt tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt
     2821 cgatctaaat tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact
     2881 ccttttaaaa aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata
     2941 gcatttttaa aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat
     3001 gaaattaagc gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag
     3061 ttaaataaag gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc
     3121 aaactttatc aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta
     3181 actaatgcta tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa
     3241 acggaagggt tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat
     3301 cacattaatt ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata
     3361 ctacgtccgt taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca
     3421 ttttcgcacg gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata
     3481 ctaataaatc aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat
     3541 gttccatgta ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact
     3601 tagtcgtggt gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt
     3661 aggctgctgg aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat
     3721 tgacaaagta tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa
     3781 atttttttac gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa
     3841 tttggaatcg tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt
     3901 tcgtgaagtg aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac
     3961 ggtatctaga gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc
     4021 tgcaattagt gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag
     4081 tagcaataca atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc
     4141 ctactatgta gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa
     4201 cggcagtgcg gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg
     4261 aattataggt attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt
     4321 cagtatagac cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg
     4381 tgggcaaatt gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg
     4441 gggtgtttca ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg
     4501 tgaggaaaat aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga
     4561 ggaacaattg ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa
     4621 tgatttcatg gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa
     4681 ggattttcaa attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact
     4741 atcaacgatt gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc
     4801 gatgatttta aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg
     4861 agagacgact aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga
     4921 aagtgatttc aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa
     4981 ataaaacgga aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa
     5041 ttgcagcaat tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa
     5101 gttcaggtag cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc
     5161 ctgaaggaaa agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat
     5221 ataaagctgt tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag
     5281 taaatgctgc aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata
     5341 ctgcagctta tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg
     5401 acatggatga tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg
     5461 cgtttggttt ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg
     5521 ctggaattac agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc
     5581 aagcaatttg ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg
     5641 cgtcgggaga cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg
     5701 atgtcgttaa tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg
     5761 aagcgttgga atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg
     5821 aaaaaggatt tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag
     5881 atttagagga aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg
     5941 caactgggga gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag
     6001 agaaaaagga tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta
     6061 ttatgagttt agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag
     6121 atgaagtgtc ggaaggaatg caggtattaa ttaagcaaaa ccaactttct ggacatgccc
     6181 gcccagtggt tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata
     6241 ttagctactt cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa
     6301 tgcaacaggc aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt
     6361 taaaaaaacc gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc
     6421 tgctgctgac gatttttctg atcatacccg taatcatggt aatttattat gcctttactg
     6481 attattattt gttaacgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac
     6541 tgttcgctga tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga
     6601 ttattccggt ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta
     6661 aaggaaatat cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta
     6721 tttcgatttt atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg
     6781 caaagattgg aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc
     6841 ttgttttcgt atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa
     6901 tgcaaaatat tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc
     6961 agcaatttcg ctatatcacc atgccattat taaagcccac ggcgattttt attttactta
     7021 ccacattgat ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac
     7081 caatgaattc tactatgaca atggtttatt acatttacca aactggattt acagaccgta
     7141 tggtaggcta ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa
     7201 ttgctcaaag aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct
     7261 aatgatcgta gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct
     7321 gtggatggta gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt
     7381 caaagctttt ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat
     7441 ttcccggttc agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc
     7501 ctgtgggtca atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc
     7561 aggcaagaaa attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt
     7621 tttaattccg caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt
     7681 gattttacca gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc
     7741 gattccagaa gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt
     7801 ctttcacatc atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt
     7861 tatctccagc tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt
     7921 ttcaatgcaa gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt
     7981 gatggctgtt ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa
     8041 atatattctg cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa
     8101 tgttgcaaaa acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc
     8161 aagaataccg gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg
     8221 gttttgttta ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca
     8281 tctggggacc aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt
     8341 tgccagtagc actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg
     8401 ccatagaaaa ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc
     8461 aagttcgaca agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg
     8521 ccggaaatcc aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc
     8581 gcgatcccaa agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg
     8641 cagatgctcg tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt
     8701 attccgtatt gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt
     8761 ttgaactaga tggcaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca
     8821 atgcttacca aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag
     8881 gcaaatttct agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc
     8941 agacctgtga aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc
     9001 gtaaaatacc tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag
     9061 agctgagggt ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa
     9121 ttgatccgca ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg
     9181 acttagtata tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac
     9241 tagaacttca ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac
     9301 gggagtgctt tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg
     9361 aagtgttgaa aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa
     9421 ttttccgtga tacttcagct attgagcttt ttctaaatga tcgtctgact atgagtacaa
     9481 ctttttatga gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct
     9541 tttcattgga aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt
     9601 tcaatcgaag caggcggcac aaaatttgtg tgtgccatta gcgatgaaga aatgacgatc
     9661 aaggaaagag tgagctttcc gacaacgaca cctgaggaaa caataccttt agtcattgac
     9721 ttcttcaaac aataccaagc agatttagca gggattggga ttggctcatt tgggcctatc
     9781 gatatccatc gcgattccgc cacttatggg tacattacct cgaccccgaa a
//
LOCUS       DASJUF010000099         9824 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJUF010000099 DASJUF010000000
VERSION     DASJUF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.100"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..564)
                     /locus_tag="U4B62_002235"
     CDS             complement(52..564)
                     /locus_tag="U4B62_002235"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN2142372.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(578..2875)
                     /gene="recJ"
                     /locus_tag="U4B62_002236"
     CDS             complement(578..2875)
                     /gene="recJ"
                     /locus_tag="U4B62_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN2142373.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3017..3460)
                     /locus_tag="U4B62_002237"
     CDS             complement(3017..3460)
                     /locus_tag="U4B62_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN2142374.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3461..4249)
                     /locus_tag="U4B62_002238"
     CDS             complement(3461..4249)
                     /locus_tag="U4B62_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2142375.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4246..5187)
                     /locus_tag="U4B62_002239"
     CDS             complement(4246..5187)
                     /locus_tag="U4B62_002239"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN2142376.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5364..5951)
                     /locus_tag="U4B62_002240"
     CDS             complement(5364..5951)
                     /locus_tag="U4B62_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN2142377.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6172..7479)
                     /gene="obgE"
                     /locus_tag="U4B62_002241"
     CDS             complement(6172..7479)
                     /gene="obgE"
                     /locus_tag="U4B62_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN2142378.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7773..8783
                     /gene="gap"
                     /locus_tag="U4B62_002242"
     CDS             7773..8783
                     /gene="gap"
                     /locus_tag="U4B62_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2142379.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8868..9803)
                     /locus_tag="U4B62_002243"
     CDS             complement(8868..9803)
                     /locus_tag="U4B62_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2142380.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2698 a         2256 c         1702 g         3168 t
ORIGIN      
        1 tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg cttaatattc
       61 cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat ccatcaattc
      121 gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt cgatcgtcgc
      181 tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt gacctggttt
      241 aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat aagtcacttc
      301 gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga ctcctaactc
      361 ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta ccatatcaat
      421 tcgtttttct tttgcataat ttacgatttg tttagtagct tcccgataag cgtcaccatc
      481 tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt cgggaatact
      541 ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac catttcttta
      601 tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg agccgtcgct
      661 ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt tgtaaaacac
      721 cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa attaataatt
      781 tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata tcaattcgtt
      841 cttgagaatg gatcaattta aacaattttg cgtactgttc tcttgtgcct accccattca
      901 aataggcctc atcaggtgaa atccccatca gataaacacg attgaatgaa taagaccgaa
      961 aaacttcttt cagatccgct aaatcatctg gacaatccat aacaactaga gagtttggtt
     1021 ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc gcagcttcct
     1081 ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa aggctacctt
     1141 ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact tgcagaccac
     1201 ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat tcattgatcg
     1261 aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga gtaccaaaac
     1321 caaaagcgac cgcatcaagc tgactcgatg catcggaaag gacaaatttc agatgctgtt
     1381 ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa aactgaggca
     1441 aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc tctactgtta
     1501 cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc aagtcaattt
     1561 gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt tcacttggca
     1621 tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct cgcatctggt
     1681 caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta gctgatccat
     1741 cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt ttcccagcaa
     1801 caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata tgattctcct
     1861 tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt ctttcttcat
     1921 tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac gtcgcaagta
     1981 acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg ggagcaatcg
     2041 aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct tcaaatagcg
     2101 cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa acaaggattc
     2161 gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact aaatccaaaa
     2221 attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact ccgactcctg
     2281 ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc gcataagcgt
     2341 ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca ccagcttgct
     2401 gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg atcaactgga
     2461 ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca tgctcaaatc
     2521 gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct ttcattaccg
     2581 ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt tctccactga
     2641 ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga tacggatcat
     2701 gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca tggtaacctc
     2761 tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc atttggagaa
     2821 aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt ctcactttct
     2881 cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga aagaaagaat
     2941 aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt gtacgaccat
     3001 ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat tgagtttttt
     3061 gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac tccgcctgat
     3121 aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct tcttttgctt
     3181 cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc agctgtttga
     3241 tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc agtgcaccaa
     3301 tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg aagccaaaac
     3361 ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa actaaaacaa
     3421 atccaagaat cacattgctt tgctttttca attccttcac ctactttttg ttaaaaattc
     3481 cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat gcaacttcta
     3541 aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg atcatttttg
     3601 ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt ccgcttggat
     3661 cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta acagctacgc
     3721 caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca aatttcgtgg
     3781 cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg atattaatga
     3841 tatgaccttg tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa accatcattc
     3901 ctaagatgtt gacttcaaac atatcgtatg cttttccaag gtcgaatgta aggaagtctt
     3961 cgaaaatccc aaaacccgca ttgtttacca gaatatcgac gcgcccgact tcttccatca
     4021 ccttttcata taattcgtcc acgctttcag gattgctgac gtctaccttg aaagcataag
     4081 cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat gccattcttc
     4141 gtgcgcaact tataacgatt gctccttgct ttgctgcttc ataacagatt tgtgcgccca
     4201 gaccgcctga actacctgtt actaaaacaa ctttatcttc taaattcatg cctcttcctc
     4261 ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt tttcaaacac
     4321 ttcttgtgct tctttttcaa gctctaaagc tgcttttcct aaatatctcg cactgatatg
     4381 agtaagtagc agccgttttg cctgcgcttc ttttgcaact tcagcagctt gatgagtcgt
     4441 ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc tttcatgtac
     4501 cagaatatcg ctattttccg caagcacaac ggaattatgt gtttttctcg tatcgcctaa
     4561 aatagtcacg attcgtcctt ttttatctgg acctacgaaa tcttttccat tgatcgtccg
     4621 cccatcttct agttggatcg tttcaccttg cttcagtttg ccgtacagtg gtccagctgg
     4681 aatacctaat tcttttagac gatccacctg caattctcct ttatgatcat gttcaaccac
     4741 acgataacca aaactgtcga tcccatgatt tagttttttt gtataaacag aaaattgctg
     4801 atcagtaaaa atcggggctg tttcatttag ctcaatgaat ttcagcggat aacttaaacg
     4861 cgtttgcgaa atacctaaag agacttttat gtattcctct atcccttttg gcccgtaaat
     4921 ctctaatggc gtatccccac cttgaaatga ccggctgctg atgagcccgg gaagtccaaa
     4981 aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg gacgaatagt
     5041 cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg aacaaccaaa cttcattgcg
     5101 ctcgtctaaa agtttcaatg ctatacttgt cacatttcta tgttttgcag gaacacctgc
     5161 acctgttcct aaaaattgta attccataat ctacctgact ttctttaagt tcctctttaa
     5221 ttttagggta aaaagaaggg ataatcaacc gtaagtcgag aatgggttca aattaaaaat
     5281 aacgttctaa agcgatagcg ttttctttga aaattactat aaaaacagca gcgaacccgt
     5341 ttgagatcct ctgctgtttt ttattaattt gaagatccag aactttcatt gtcgttggaa
     5401 gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg attttcagct
     5461 tctttcgcat catgcacatc tttttcgaca tcttcataat ccaagcgagt aatttctccg
     5521 cctaattcca tcattaccaa ttgattcagt gggcgattgt cctcttcatc agcaatcaag
     5581 atcagtcctg tagctccttc accgatttta tttcctacaa agtcgaacac ggtctgggca
     5641 tcttttattt ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa ccatcctaat
     5701 aaaatcccta gcggaccacc taatacacca accaacattc cgattaatcc acccgtagaa
     5761 gatttgttat taccggtaaa gtcgataaaa tcttctattt tgaattgatg tgtcccatta
     5821 ggatcatggc tgacaactgc catctgttct cctttaatcg aacgaacagc atgtaacttt
     5881 ttgatttctg aaaatgcctg atatgcctgg ctttctactt gaaaatttaa aatgattact
     5941 cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt atttatttta cctttaattg
     6001 aaaaataaga aaagaaaagc actctttttt tccaataaaa tagggctgtg acaaaagtcg
     6061 tatcacagct cctttttccg aataaacggc gttcttgaag ctgatcactt ctgtcacaac
     6121 ctcccttaaa tctgaatcta tattttttgg tatttgtttt tatttgcttg cctattcgac
     6181 aaattcaaat tcaaactcac cgatacgtac aaggtcgcca tctttcgcac cgcgtgcccg
     6241 aagagcttca tccacaccca taccacgtaa ttgacgagca aaacgcatca ctgtttcatc
     6301 atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc tcaccagata agatccacgt
     6361 tgcatctgaa tcacgatcga tttggaactc aggaccttct ggattgaatc catagtgtac
     6421 agtttcttct tccagttctt cttcgtacaa tgggaattct ggcgttactt ctaacaaatc
     6481 tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa ttggaaatac
     6541 tgggatgtca tcagcaaatt catcttcttt ttccttgttc agctgttctt tgaatttcac
     6601 tagattttcc ggtgcatccg gcatatccat tttgtttgcc acgatgattt gcggacgctc
     6661 tagcaagcgt aagttataag tagataattc tttgttgatg gcaagatagt cttcataagg
     6721 atctcgtcct tccatcccac tcatatcgat cacatgtaaa attactcgtg ttctttcgat
     6781 atgacgaagg aattgtgtac ccagcccaac accttgcgaa gctccttcaa tcaagcctgg
     6841 caagtctgct gcggcaaaac ttctgccatc acttgtagta accattccta gattaggtac
     6901 gagcgtcgtg aaatgataag cgccgatttt tggacgagca gatgaaataa cggatagtaa
     6961 agtcgatttc ccgacagatg gaaaacctac aagtccaacg tctgccaata ctttcaattc
     7021 tagttcgatt tttcgctctt gtcctggttc accgttttca gcaatttccg gtgcaggatt
     7081 ccgaggagag gcaaaacgga tattccctcg tccaccgcgt ccaccttttg ccacaactag
     7141 tgtttgacca ttttcaatta aatctcctaa aagagcacca gtctccgcat ctcggacagt
     7201 ggttccttgt ggtactttaa cataggtatc ttctgaacca cgaccgtgca tccctttact
     7261 catcccattt tcacctggct gcgctttgaa atggcgattg aaccgaaaat ccatcaatgt
     7321 acgtaaccct tcatcgacga tcaagatgac atcgccgcca cgaccaccgt ctccgccagc
     7381 cggtccgccg tcaggaacgt atttttcgcg acgaaatgca accattccgt ctccgccctt
     7441 gccagctttc acatcgatcg ttacttgatc taaaaacatg gacataatta agtcctccta
     7501 ttattcattt atttatttga ctgcctgaat ttccacaata aacaaaagtc ttacttattt
     7561 tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa gattgtttac cttcttcttt
     7621 ttttatcatt tggtaaggtt tccaaccttt tttctgctac caagtataat tttttgacaa
     7681 aatattcttt gcgtagcaga ataacagtgt attaattgta ttaattgagg atgccgtttc
     7741 acagcacccc taataaagga ggaacttttg atatgacagt aaaagtaggt attaacggat
     7801 ttgggcgtat tggccgtctg gctttccgcc gcatcaaaga agtctcagat gatattgaag
     7861 tagtagcaat caacgactta acaagtccaa ctatgttagc acaattattg caatttgact
     7921 ctactcatgg tacttatcct ggcacagtta ctgcaacaga agactcaatc gtagtggatg
     7981 gcgaagcaac tcgtgtttat gcagaacccg acgcaagcaa aatcccttgg gcaaaagaaa
     8041 acggtgtaga tatcgtacta gaatgtactg gtttttatac atctgaagaa aaagcaaaag
     8101 cgcatttaga tgctggtgtc aaacgagtag ttatttctgc cccagctggt gctatgaaaa
     8161 ccatcgtcta taacgtcaat gatgatacat tagatgaaaa cgacaaaatc atctcagcag
     8221 gttcatgtac aacaaactgt ttagcaccta tggcttattt cttaaataat gaatttggga
     8281 tcgaagttgg tacaatgaca acagttcatg cctacacttc cacacaaatg ctcttagatg
     8341 gacctgtaaa aggcggtaat ttgcgtgctg ctcgttcagc tgctgacaat actatcccac
     8401 attctactgg tgctgcgaaa gcaatcggct tagttatccc tgagctacaa ggaaaattac
     8461 aaggacacgc acaacgtgtt ccagtcgttg atggttcatt aactgaattg gtttcagtat
     8521 tgaaaacaaa agtaactgct gatcaagtaa acgaagcaat caaaaaacac acagttgaca
     8581 atccttcatt cgggtatgat gaccgtcaaa tcgtttctag cgatgtcatt ggaacaactc
     8641 aaggttcgat ctttgaccca acgcaaacag aagtaacaac tgctggtgat ttccaacttg
     8701 ttaaaactgt tgcatggtat gacaatgagt atggatttac ttgccaaatg attcgcttac
     8761 tagaaaaatt cgctaacttg taatttgata gatcaatcga taaaagaagc ctttgactgg
     8821 atccagtcaa aggcttcttt cttttcatcc cctactttta aactcgttca atgaacatat
     8881 tggcttaaaa attgttggac tacttgctga tatttttcag gatctgtctt gtatgcttcc
     8941 gcatgctcgg ctccttttac aacatacttt tcttttggtc ccttcgttgc acgatagacc
     9001 ttatctaaca tttcaaatgg tacaaatgta tcttcgtctc catgtataaa gagcattggg
     9061 cgctggtttt tcttcaattg ttccactgca tttgcttcac cgaaaaagta gccagcacgg
     9121 acttttgtca ctagactcgt tacttgcata agcggaaatg ctggaagact aaacatatcg
     9181 tctagctgat aagcaagctc atcatgaacg gaagaataac cgcaatcttc aatgattgct
     9241 ttcacattat ctggtagttt ttctccgcta gtcatcataa ctgttgctgc tcccatgctg
     9301 acaccgtata gaacgatttc ttgcgatttt ccattgtttt ctagcacttt gtttatccac
     9361 tgtacatagt ctttacgctc tggccatcca aatccaatgt aatccccttc gctttttcca
     9421 tgtcctcttg catcaggaac aaggacattg taacccatat catgaaacat ctttgcgtaa
     9481 ttggtcatcg tttcagcgtt tcccatatag ccatgagcca caagagctgt tttttcactt
     9541 ttcgtttcag caggaagata aatcgctgat aatttcagac cgtcttttga agtgatggtc
     9601 caatcgcttc ggttttcttt tgaactaaac catttatgtg catccgtttc tttggatgtc
     9661 tgtgttgtac caggtgtatc ttcgtctaaa aagtctttct cagaaggcac aacagcataa
     9721 ttataaaaat aattccctgc aaacactaac gcaacaatca gagctaaagc gacgcctaaa
     9781 aggattttca tccaaagttt cataaatcat ccatctcatt tctc
//
LOCUS       DASJUF010000100         9811 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJUF010000100 DASJUF010000000
VERSION     DASJUF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.101"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..2154)
                     /locus_tag="U4B62_002244"
     CDS             complement(34..2154)
                     /locus_tag="U4B62_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2142381.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="U4B62_002245"
     CDS             2384..3562
                     /locus_tag="U4B62_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN2142382.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="U4B62_002246"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="U4B62_002246"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN2142383.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="U4B62_002247"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="U4B62_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN2142384.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="U4B62_002248"
     CDS             complement(4915..6168)
                     /locus_tag="U4B62_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2142385.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="U4B62_002249"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="U4B62_002249"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN2142386.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="U4B62_002250"
     CDS             7746..9092
                     /locus_tag="U4B62_002250"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN2142387.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="U4B62_002251"
     CDS             complement(9400..9786)
                     /locus_tag="U4B62_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2142388.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DASJUF010000101         9681 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJUF010000101 DASJUF010000000
VERSION     DASJUF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.102"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            282..521
                     /locus_tag="U4B62_002252"
     CDS             282..521
                     /locus_tag="U4B62_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142389.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="U4B62_002253"
     CDS             complement(883..1143)
                     /locus_tag="U4B62_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142390.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="U4B62_002254"
     CDS             complement(1328..1825)
                     /locus_tag="U4B62_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN2142391.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="U4B62_002255"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="U4B62_002255"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN2142392.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="U4B62_002256"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="U4B62_002256"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN2142393.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="U4B62_002257"
     CDS             complement(4547..5209)
                     /locus_tag="U4B62_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2142394.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="U4B62_002258"
     CDS             complement(5219..6034)
                     /locus_tag="U4B62_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2142395.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="U4B62_002259"
     CDS             complement(6296..6739)
                     /locus_tag="U4B62_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEN2142396.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="U4B62_002260"
     CDS             complement(6873..7211)
                     /locus_tag="U4B62_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2142397.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="U4B62_002261"
     CDS             complement(7199..7576)
                     /locus_tag="U4B62_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2142398.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="U4B62_002262"
     CDS             7800..8993
                     /locus_tag="U4B62_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2142399.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="U4B62_002263"
     CDS             complement(9159..9581)
                     /locus_tag="U4B62_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142400.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2890 a         1932 c         1590 g         3269 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta t
//
LOCUS       DASJUF010000102         9436 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJUF010000102 DASJUF010000000
VERSION     DASJUF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.103"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            15..200
                     /locus_tag="U4B62_002264"
     CDS             15..200
                     /locus_tag="U4B62_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142401.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            213..461
                     /locus_tag="U4B62_002265"
     CDS             213..461
                     /locus_tag="U4B62_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142402.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            492..662
                     /locus_tag="U4B62_002266"
     CDS             492..662
                     /locus_tag="U4B62_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142403.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(764..1222)
                     /locus_tag="U4B62_002267"
     CDS             complement(764..1222)
                     /locus_tag="U4B62_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142404.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            1274..1972
                     /locus_tag="U4B62_002268"
     CDS             1274..1972
                     /locus_tag="U4B62_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN2142405.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2150..2491
                     /locus_tag="U4B62_002269"
     CDS             2150..2491
                     /locus_tag="U4B62_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142406.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2484..3155
                     /locus_tag="U4B62_002270"
     CDS             2484..3155
                     /locus_tag="U4B62_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN2142407.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3161..3853
                     /locus_tag="U4B62_002271"
     CDS             3161..3853
                     /locus_tag="U4B62_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142408.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            3850..4599
                     /locus_tag="U4B62_002272"
     CDS             3850..4599
                     /locus_tag="U4B62_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142409.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            4611..4880
                     /locus_tag="U4B62_002273"
     CDS             4611..4880
                     /locus_tag="U4B62_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142410.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            4886..5050
                     /locus_tag="U4B62_002274"
     CDS             4886..5050
                     /locus_tag="U4B62_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142411.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            5043..5342
                     /locus_tag="U4B62_002275"
     CDS             5043..5342
                     /locus_tag="U4B62_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142412.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            5342..5644
                     /locus_tag="U4B62_002276"
     CDS             5342..5644
                     /locus_tag="U4B62_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN2142413.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            5684..6049
                     /locus_tag="U4B62_002277"
     CDS             5684..6049
                     /locus_tag="U4B62_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142414.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            6539..6679
                     /locus_tag="U4B62_002278"
     CDS             6539..6679
                     /locus_tag="U4B62_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142415.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            6676..6858
                     /locus_tag="U4B62_002279"
     CDS             6676..6858
                     /locus_tag="U4B62_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142416.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            6871..7065
                     /locus_tag="U4B62_002280"
     CDS             6871..7065
                     /locus_tag="U4B62_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142417.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(7098..7310)
                     /locus_tag="U4B62_002281"
     CDS             complement(7098..7310)
                     /locus_tag="U4B62_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142418.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            7435..7638
                     /locus_tag="U4B62_002282"
     CDS             7435..7638
                     /locus_tag="U4B62_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142419.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            7635..7907
                     /locus_tag="U4B62_002283"
     CDS             7635..7907
                     /locus_tag="U4B62_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEN2142420.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            7908..8093
                     /locus_tag="U4B62_002284"
     CDS             7908..8093
                     /locus_tag="U4B62_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142421.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            8080..8427
                     /locus_tag="U4B62_002285"
     CDS             8080..8427
                     /locus_tag="U4B62_002285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142422.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            8387..8863
                     /locus_tag="U4B62_002286"
     CDS             8387..8863
                     /locus_tag="U4B62_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN2142423.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(9009..>9436)
                     /locus_tag="U4B62_002287"
     CDS             complement(9009..>9436)
                     /locus_tag="U4B62_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEN2142424.1"
                     /translation="DLPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLI
                     KDYAISKNSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNK
                     DVHDMDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
BASE COUNT         3465 a         1413 c         1927 g         2631 t
ORIGIN      
        1 aaggagctat ttttatgtct gagaatttag acttaaaaat tcgagcggag atgagaaaaa
       61 gaagaatgac tttcaaagaa ctagctgcgc ttatcggtat ttcaggagct tatttatcag
      121 atattctaaa cggcaatcgt gatggaaaga aagcacaaca gcatatcgaa acagtgaaaa
      181 aaatattgga catccgatag ggggggtagg cgatgacaaa actaaaaaaa caagattttg
      241 taaaaaaata taattattct ccatctactt atcaacgtcg aatgtcggaa ctaaaaaata
      301 cagcaatttt ctcagcggcg tatgaacggg tcacaggaca agaagtttgg atcaatacag
      361 aattatacga taaatttttg tctttcaaat cctataacag gttacgcaca agaaaggtaa
      421 cacctaaaga atttatcgag aagcatttag ttgatttata aaaaacagaa cattttgaga
      481 ggtgaaggtt aatgggtaaa ttcaacagag cattagtatt cagcgcaccg ctaatcgtct
      541 atgctttagg gctttgggga agtagacaag cgttgatagg aacgattgtg tacatggttt
      601 ggatttttat agggctggat gaagctgagt acaaaacaaa aaagccagtc gggagggact
      661 gactaatgag taaggacgat attgtatttc tgttaattat gttttgtggg attggatcaa
      721 taataattaa tttgtatttg attattaaag aaaaattttt caattaaaat gagtctttta
      781 gctttaactt aaattttttt tgataattag ctgttcttat gaccattaca agtttttcac
      841 caggtaatat atctttcata tctaaaactt ctgaaaaagc gactgagtag ccaggagaga
      901 ttgcttgtgc caacggtata ggagataaaa ggctactttt atgaatgctt cccatatcaa
      961 aaccattggc gtgataatgt gaacaattaa ttttcttacc gttgtaaaga tacgtacatt
     1021 cgataattga gtttggcatt tttgatgggt ttgcaattac gccagtaaga atcattcgat
     1081 tacttctatc atgcttaact aaaaaatagg agagcgtaat ttttctttta tctttaaata
     1141 agttatagag ggataaaaaa aatcctgaaa tagaaatgat taaagtaata gcgacttggt
     1201 aatcgctcaa aatttttatc aagtaattca ccaccttttt actcaaatta tatcaaaaag
     1261 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
     1321 cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc
     1381 actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc
     1441 atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat
     1501 ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga
     1561 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta
     1621 tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg
     1681 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt
     1741 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
     1801 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
     1861 attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga
     1921 aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag
     1981 cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc
     2041 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
     2101 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
     2161 actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga
     2221 aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca
     2281 agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa
     2341 gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga
     2401 agagtacgac agagataact tgattgatat ctcttattac ggaccaggta agaaatacat
     2461 tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac
     2521 tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt
     2581 gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
     2641 agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
     2701 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
     2761 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
     2821 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
     2881 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacaa
     2941 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
     3001 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
     3061 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
     3121 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
     3181 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga
     3241 ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat
     3301 tgaaattcgt tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc
     3361 gctgataggc gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt
     3421 ctactttcgt tttgaaggtg tcacagggcg caaaataagc ctgaaagacg aatcgaatac
     3481 aactgtgagt gatgccaatg tcttagcaaa tatcatctta gatttcatct ttgaacatca
     3541 tattcctttc aaagaaggct atgagatttt acctgcgaat caagaatatt acttctacaa
     3601 atgcatcaca aaaagagtct gctgcatctg tggcaaaaca ggagctgaca tcgatcactt
     3661 tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat catgcagagt acacatttgc
     3721 agcactctgc agaatccatc acacagagaa gcacaaaata ggtgtgatta atttcaaaaa
     3781 taaatatcaa atcaaaggga tcaaattaaa ccagaaaaca atcaaaaagt taaatattgg
     3841 aggggaaaaa tgacagaaca tcgaagttat tatgcgatta taccagccaa cgtaaggtac
     3901 gacaaaagac ttaaaccaaa tactaagttg ttatacggag agataacggc cttgtgtaat
     3961 gaaagaggct tttgttgggc aggcaatgag tactttgcag atttatatgg tgtgaataaa
     4021 gagaccatat cgcgatgggt aagtgatttg attaagtttg gatacttgaa tcgggaaatc
     4081 atttacaaag agggtaccaa tcaaataatc aataggtacc tacgaattaa tcaataccct
     4141 attgacgaaa aacgcaatac ccctattgac gaaaaagtca aagataataa tacatctatt
     4201 aataatacat ttaataatac aaaagaatat ataagagagt taccgccttc gaaaaaatcg
     4261 aaggctaagc ccgtccgtca taaatacgga gagtataaaa atgttctttt gtcagatgag
     4321 cagatggaga aactcaaaac agaattccct aatgattacc aagagcgaat cgaacgactg
     4381 tcagagtatt gtgaatcatc tggtaagact tataaaaact atttggcaac tattcgaagt
     4441 tgggcaagga aagaaaaaag tgaacctaag aacgcaagca gtggatacaa gcgcacagga
     4501 agacgagaga agcttcctga atgggcaatc gaccaagaag cctatctcaa gaaaaaagcg
     4561 ctagaacgag ctaatagaca atcaaaagca ccattctaag aggtggaaaa atgaagatcg
     4621 attatctaga actaattaat gaaatagcga attataaaaa gggcgaggaa ttagacgtcc
     4681 tgagagacgt atatgatcaa ctcgaagaag ctggaattga aggaattaag aatgatcgtt
     4741 cgagttggag taaactcaga tactatttcg cactctatat cgatggaacg caattaagaa
     4801 atttagcata taccaaatta ctattcattg attgtgttaa aggcttgcaa aaacatctta
     4861 atgaacttga gcaggtgtaa acaagatgga cctaaagaca tttacagcac agatcgaatt
     4921 aatgcatcaa gaagctttaa gacaaagtgt gtcgtacgaa gacaagtggc tcaacacatt
     4981 tcatggcgga cgtgagagcg cacttgatca agtactcaaa ttactgaaag gagaatgtcg
     5041 ggatggataa gaaagcggca atgcagcgaa ttatcgaatt gacttattca gaagattggc
     5101 aggaagataa aaaaatagtt gcagaagtcc aaaagctcgg taaatcaatg tggactgaaa
     5161 agcccaaacg gagaacgccg agaaagattg caatctggca tggtgatcga attctagtaa
     5221 cgggtaccgc taaacagtta tctgaaatta ctggtctgag caaaaatatt atttgggata
     5281 gagctaggag cttatggatt gattcaaaag gaagacagtt taggtatgtg gaggagaaat
     5341 aatggacgaa ctaatcgcga aagtagagca gtgggcaaaa gataagggat tggatcaagc
     5401 ggatttcagc aagcaaatgt taaaaacggt cgaagaaatc ggggaagttg ccgcttctct
     5461 agctagaaaa gatgaacatg gtttaagaga cggaattggc gatgtagtag taaccttgat
     5521 catcttggca atgcaaaatg atatggattt atacgagtgc ttggattttg cttatgacga
     5581 aatcaaaggt cggactggaa agatggtcaa tggtgttttc gttaagtcca gtgacctgaa
     5641 ataattccat taatggaaga aataactgaa taggaggata gacatgattg atgcaaaaca
     5701 agtgcaaaaa cagaaagatg gaatgttgat gtttgaagca tatgttctac catttcttaa
     5761 tcaatttgaa gttttggaat gctctgccag cggagaagaa ttggagtatg ttgtaattcg
     5821 tgaaaccaaa gaaaacgttc agaagctaaa cgaatttctt tgcactatta actgttggga
     5881 tatgatagct ccaggatttt tatgtccagc tatgggagaa tttttggaat attgtcgtct
     5941 tgaagatgca ggcactttgg atttagctta tctggtatac aactacctaa acatcaacac
     6001 agatcatttg tggtttggca ctgccgaaag aaaatgggta gtccgctaac caccaatatc
     6061 aatgaaaaaa ggcggtttcc tgaaagtgga attgccgcca ttgctgtaag gttctttacg
     6121 cgactcaagg tcacagaaat ataaatgcaa atctcttttg aagtgatcca aagctctcgg
     6181 aaaatggaag ttttttgttt caaagaataa atgagatttg cgtcaagaac ctgagagtta
     6241 tttccaaaaa agcttccaaa gaaccgaatg agctaaggac atcatgtgta aacacataat
     6301 atagctctcc tttcacggct aatcataaca tgaaaaatat tcgatgtcag taggaaaaaa
     6361 ggtgaattat gaaaagaata ttaaattatc ccggcagtaa cagattctgc aattaaactc
     6421 cgaagctgat gtatcagtat caactgtaga atattatgag cgtggagaaa gtcaagcagt
     6481 cacaaatgta aaaatcgata acgtcaacgg tcttatctgt ttagatgaag gcgatagtat
     6541 gaatgaaatt gctgcagacc atgaattatc agaaactgct ttaattgatc aaatcgaatt
     6601 agctagacaa tactttgttc catatgcaga tacctggtcc aagcataaga atgacatcat
     6661 ttttccagag gaacaatgag agcaactgat ccagtaatta tccttgagga agccaaattt
     6721 atttggactc acgaagagat agagcaagca cgcttgctct tttctcaagg agttaagccg
     6781 agcaaagtag ctgaaataat gggtcaaaag attcttgatg tcggattgct tttgctccat
     6841 ctagcagaaa aaatttgatt tgaggtgaaa atgatgattc aacttgcagg catccaaaca
     6901 gggaaaattt atttttctgg agaaagcaaa agcgaagcca accaatggct attgaaaact
     6961 tacacgaaca ataagaagct aaggaaaaaa tatcctgatt tgttgctaaa ggatgatcag
     7021 attatgccgg aaccgatgat tttgacccgt aaggagggaa gttgaaacta aaaaaccagc
     7081 cgtgtgggct ggagttgcta tttcttttta ataatgagtt tttctaaatt taattcattc
     7141 tcacgagttt gcgtccaata atcagaataa tattttaatt gtttaagagt aggaatgtcg
     7201 tcttcggtaa aattagaaaa taattctgga gaattatctt tttttaattg ttctaatcct
     7261 gattctttaa aacgttgttc aacgagtttt actaaattgt tgatattcat gataaatcac
     7321 ctttttaaat ttaatttcga tttgattaat cgatacaagt attatatcaa ataaaaaagc
     7381 tgatttaggg ggtcttacaa aaacactaaa attttttcaa aaagaggagg aacggtggtg
     7441 aaagtcagag aacaattcaa gatagtgagc gacgaaggta atgtttacaa aataaagatc
     7501 gttaatatta atgaatatcg agaagcagat cacaaatatt tggttgactt atacatgaac
     7561 gataaatatt tgggagaagg attttgtgga gattcacttt ttgatgatga gcgaattttg
     7621 aaggaggaaa cagaatgatc cacgaactaa aaatactacc agaatatttt gaagcagtga
     7681 cgagcggacg caaacaattc gagatccgca agaatgatcg tgacttcaaa gttggtgatc
     7741 agttaatact acgtgaatat aaaaaataca ttaaacatga attacctgga ctaacgagac
     7801 caacatttag ttacactggt gactcataca aagcagagat cacttatatt actgattaca
     7861 tgcagaagtc cggatatgta gtgttgggga ttaaaggggt gaattaaatg aaagtataca
     7921 tcttagctat cacagagggc acatggatgt tcccagtcgg ttccggaaaa atctataaat
     7981 caaaaacagc tgcttataaa gcatttgaaa aatacaaaaa agaaaacggt ggcggcacta
     8041 acgcaaagat tctagtggct gataactggc atgaagaagg tgaacgtaat tgatttacct
     8101 gttcattttc gtggtactaa ctatttttat gctagtaatc ttattcaaaa aaagatgagt
     8161 aaagacgaac gaaaaattct ggccaacttt tatttagtat taaaaatcga acaaaatgtg
     8221 ttttcggaaa ggagaattgt atgggaaaaa caaaatcaaa gattaagaag aaaaaacgac
     8281 gtttggaaca aaaggcaatc cagaatggaa cggctaagaa aaaataaaaa agctacccct
     8341 ttcgagatag cagcgcaata ctattctacc ataaggggtg gcgtttgtga gatttcattg
     8401 gttaaaagat taccaagaac tagatgagca gattctttac ttaaagtgga atcttaataa
     8461 aagtaagctt gaattgaatc gatgggtctg tggtgattta gcaaacgtcc gcatcgaaaa
     8521 gaattcgaga acttcttctt tagaagagaa tatccaaaag atagaaaatg aattggaatt
     8581 gttgatcgag caaaaagaag aaatgttatt gctgatagat agcttttctg gtatcgataa
     8641 tcaaattgtt aagatgaaat atgttgatca aatgagctta gaagatattg ctgaatctgt
     8701 tgggtatagc tcatcgtatg tcagacagcg acatgcagaa attagaaaga cattgaactt
     8761 tttagatgag tatgaacaca gacaagctga ccgattgaaa aaagaaaacg aaattgattt
     8821 ttacaatagc gagaagtaca aagaacaact ctctttattc taaaattaca ctgttcacta
     8881 aatgttcggt cattgtagct atgtaaacat tgttagcagc atgatattct attagtgtca
     8941 aaaaaatatg aaagagccaa gatatcccaa ccgttttatt aattggtatc tgtggctctt
     9001 ttattctttt aattagtagt tggcataaag ttatttccat ttataaattg atcagctaaa
     9061 tcagcatcat aatcagccgc gataaattgc gctgctgatt taataaatgc atccatatca
     9121 tgaacatctt tattagtatg tctacgaaca tagtgcgttt catcattacc aatccaagta
     9181 gcagctttgg ctaatctttg gagtttagga aaatcattca aataattttc aattgtttta
     9241 cctaataaca ttttttttat tttatcttca tcctctgaat ttttagatat tgcataatct
     9301 ttaattagaa attctagagc ttttctataa ccaactccag caatttgttc caattgctca
     9361 gatttagctt gtgtggcttg agtataaata tcaacaaatg taggagaaac tttttcaata
     9421 ttttctggta aatcta
//
LOCUS       DASJUF010000103         9004 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.104, whole genome shotgun sequence.
ACCESSION   DASJUF010000103 DASJUF010000000
VERSION     DASJUF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.104"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..311
                     /locus_tag="U4B62_002288"
     CDS             <1..311
                     /locus_tag="U4B62_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HEN2142425.1"
                     /translation="AEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRL
                     QNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD
                     "
     gene            346..1113
                     /locus_tag="U4B62_002289"
     CDS             346..1113
                     /locus_tag="U4B62_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2142426.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1305..1703
                     /locus_tag="U4B62_002290"
     CDS             1305..1703
                     /locus_tag="U4B62_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN2142427.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2010..2384)
                     /locus_tag="U4B62_002291"
     CDS             complement(2010..2384)
                     /locus_tag="U4B62_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142428.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2406..2783)
                     /locus_tag="U4B62_002292"
     CDS             complement(2406..2783)
                     /locus_tag="U4B62_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142429.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(2773..3168)
                     /locus_tag="U4B62_002293"
     CDS             complement(2773..3168)
                     /locus_tag="U4B62_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN2142430.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3235..3420)
                     /locus_tag="U4B62_002294"
     CDS             complement(3235..3420)
                     /locus_tag="U4B62_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEN2142431.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3502..3756)
                     /locus_tag="U4B62_002295"
     CDS             complement(3502..3756)
                     /locus_tag="U4B62_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142432.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(3778..4023)
                     /locus_tag="U4B62_002296"
     CDS             complement(3778..4023)
                     /locus_tag="U4B62_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142433.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4030..4314)
                     /locus_tag="U4B62_002297"
     CDS             complement(4030..4314)
                     /locus_tag="U4B62_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142434.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4417..4599)
                     /locus_tag="U4B62_002298"
     CDS             complement(4417..4599)
                     /locus_tag="U4B62_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN2142435.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(4805..5344)
                     /locus_tag="U4B62_002299"
     CDS             complement(4805..5344)
                     /locus_tag="U4B62_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142436.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKIYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(5457..5723)
                     /locus_tag="U4B62_002300"
     CDS             complement(5457..5723)
                     /locus_tag="U4B62_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2142437.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5725..5946)
                     /locus_tag="U4B62_002301"
     CDS             complement(5725..5946)
                     /locus_tag="U4B62_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEN2142438.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            6172..6990
                     /locus_tag="U4B62_002302"
     CDS             6172..6990
                     /locus_tag="U4B62_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2142439.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            6994..7290
                     /locus_tag="U4B62_002303"
     CDS             6994..7290
                     /locus_tag="U4B62_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142440.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(7472..7825)
                     /locus_tag="U4B62_002304"
     CDS             complement(7472..7825)
                     /locus_tag="U4B62_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142441.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(7845..8900)
                     /locus_tag="U4B62_002305"
     CDS             complement(7845..8900)
                     /locus_tag="U4B62_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HEN2142442.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
BASE COUNT         2899 a         1454 c         1574 g         3077 t
ORIGIN      
        1 gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg aatagcaaat
       61 acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc gatcttaaac
      121 ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc aaagatagag
      181 gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag gattacacaa
      241 taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct tatgtgaacc
      301 tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa aaaagatgaa
      361 ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga tttaaagtcg
      421 tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa gaaaacaggc
      481 gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca aaatcttgaa
      541 acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga aatttcgaat
      601 ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat tcattgggat
      661 ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt aattgtctca
      721 atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca aaaaaaatat
      781 agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat cgccaaaaat
      841 aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat agttaaaatt
      901 cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga tagacgtgtt
      961 ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga ttttgctaaa
     1021 gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag tgagtttgat
     1081 gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac tcgtttgaag
     1141 atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta atctccttta
     1201 gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct tttttttgaa
     1261 acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa tacttttttt
     1321 gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat tttccagact
     1381 tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa gtattggaag
     1441 gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg cctgttcaag
     1501 agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta aaagctaata
     1561 cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg actgtggaac
     1621 actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct ttgaatgatt
     1681 tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa aaacagcttg
     1741 ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa aagctctcag
     1801 aagccttctg agagctttta aattgctatg tattgtactg catctgctca aattgatttt
     1861 cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata tgtattgagt
     1921 aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat gtgttaggat
     1981 aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt tttatcggta
     2041 ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat ccctgcatcg
     2101 ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat caaaatggtg
     2161 tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga cttataaaaa
     2221 agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta tttccaaaga
     2281 tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc tgctaattga
     2341 tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct cttctcctgt
     2401 ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc ggtttgaaat
     2461 tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga ttcacttaat
     2521 gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt atagcagaat
     2581 tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa gttattatcc
     2641 tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc agttgtatct
     2701 tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc tttcataggt
     2761 tcggtctcct ttttactttt caagcaactc ttcttccagt gctttttgaa gtagttgaga
     2821 gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac taacattttt
     2881 tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt ggatgattac
     2941 taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag gaaattctgc
     3001 atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat atgcttcttc
     3061 aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt tataagcttc
     3121 tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt cctcgctcct
     3181 tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag gctgttaaag
     3241 ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat tgtggatcgg
     3301 aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc cagtttgtcc
     3361 agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg tcttaggcat
     3421 agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat caatagaaaa
     3481 agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag caaattgaat
     3541 gttattagcg ttgcaaatat tcatgatttc ccaagtatca tcagaagtat aggtatctaa
     3601 gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt tctccaagtc
     3661 ttttgatgaa ataatcaaga ctttttcaat ttcctcccct tcttcatcta tgaaaacata
     3721 ttctaaagtg atttgttctt catcggtata attcatattg tgttctcctt tcttgtacta
     3781 agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata ggacgtgacc
     3841 gagcgttacg cctttgaaat gttcttctaa atacatccaa acgcattcaa ggctagtacc
     3901 tgctttgaga ttgtagaagt ctgcatccaa taccttttca gaagttatcg gatcaatgcg
     3961 agttggtact tcaataaatt cagtccaaat ttctgcaaaa gtcttacctt ccaaagtcca
     4021 cactccctat caattaattc caaaagcaaa tagagaaatc aagcgaccgt caatagtcac
     4081 ttcaacaact gaaaatgccg ttgaaacagt tttaacatct acactttcca agttcccgat
     4141 gaaggaatct ttgtgcttta gaatagcttt ctttagctgt ctgttataca cttgccgact
     4201 gttaaacttt ttgaattctc gatccttgat cagctgttta acgatccctg cattaattgt
     4261 ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa gtagctgtta gcatcttgtc
     4321 actccttcct aaaagttaat tagcttgttt acaaatacat tatacacatc ggtgtgtata
     4381 atgtaaagag aaaaaggtgc taatggatca gctgttctaa acaaaagaaa acgataaagt
     4441 aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct agtccggttg ctttgcttgt
     4501 gtgattttcg gctttcttat agatcaaata ggaaattatc agtgatattc cagtaaagaa
     4561 taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc cttctgtaat
     4621 attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcagat ggtgatgttt
     4681 ttagccctac cctaaaaaca aagcccagaa aagaatttaa aagcgttcgt acctaatacg
     4741 tttatattca tatacatata cataaacata aacgtgtata cgtttatgtt tatgtattta
     4801 agttttaggc tacttcctta acatctcctg ctgatagtcg agtgaaccga gtgagtgatc
     4861 catcttgtag tcgaaaaata aagctacgtt tgagtttata ctgatagaca gcgacatacg
     4921 atccatccca gattgagtaa gttttacctg caactaataa gtcatcaagt tttggtttat
     4981 tgctttcttc tttaattcta tctagttgtc gcttaaccat tttttcttga tcaccgacaa
     5041 agaatttcaa ccattttttc ggaattttct taaagtaagc aggactgtca tttaagaagt
     5101 agattggttt gtacatttca tctcgaaaga tgaagctgtc aatatcgtag gttccttctg
     5161 catctttgaa aaggataatt ccatagatct tcgttacttt ggtatctacg atttccaaac
     5221 catttttggc gtaccctttc ttttcttcga tcacttcatt tgctgatgaa taatcaccat
     5281 accatcccat aaaaaatcgc tccttttgaa agttaattag cttgtttaca cttatattat
     5341 acacaccggt gtgtataata tcaagaggat attgttcttt tttaggtaaa gtgggatcgt
     5401 aaacagtgat tttgttgggt taacaaaaaa acagacaagc cttatcagct tgtctgttac
     5461 ttataaactt cacttctgtg accaatagac aatataagaa ttgtcacttc atgctcgcta
     5521 atatcagcta ttatacgata tgcacctacc ctatatcgcc agtagcctga tttattagct
     5581 gttagacctt taccatgctt tcttggatca tcagtgccta caagattttt ctcgatccag
     5641 tttaggataa cctttgcctg aaacttatcc atttttttta gcgctttttg agcttcacgt
     5701 tcatatctta cttggtaagt catcttataa gcctaactct tttagcatat cttcgtggga
     5761 aatactttga tcgttttcct catgctcttt cattagttgc ttgtaggtgg caaagtctac
     5821 taacgtttct gctgtttgca gggaattact acgcataaat tcagaaatgt tatctccgtt
     5881 gaattcagca actttttcaa gaaaatcttt ttcttcttta gatacacgaa tacttattac
     5941 tggcatagtt tatcaactcc ttttcaatta cattgtacta cgattgtatg acaaatgcaa
     6001 tagttatata tttgaatata tacataaaca taaacatata catatatatg tttatgttgt
     6061 agaatataaa agcaaatata aatataaaca cgtttatgta tataaacata tacataaacg
     6121 tgtttatagt gtataatgga gttacaaaag gttaataagg gaggtttaat catggaaaag
     6181 aagttgtcaa tcattactgg aaacattgaa aaaggtggaa caggtaaaac aacaacaatc
     6241 tacaatctag gtggatatgc tgtcgaaaat ttaggtatga aggtgctgta tattgatgaa
     6301 gataagtcgc aaaacctatc aaaacgattt cctgatgctt tcagtaaagc gaaaagagag
     6361 aatacaatca atcatttata tcttcatgga tcagctgaac ctttagctat aaatgagaac
     6421 atggatattt tagtagcagg tgaagattta aaacaagttg agatgcaaat gcgtgatatg
     6481 cctaacaaca gaaccctact gtttagatgg atcgttgtta actatgaaca gcttaaagag
     6541 aagtatgatc taattctgat tgatactcat aatgatgcag gtctactagc tcaaaatgct
     6601 tgggctgtat ctgatcttgt cgtaggcatt tctgatccat ctaaagatgg ttttgaagct
     6661 ttgctgaagc ttggtaaaga tattgatgca ctagcaaaac agttgattga tattaagact
     6721 ggtgaaacac tgatggaaac taaatacgct acgatcggta gcaggatcaa acataatact
     6781 aattcaagtc gtgagttcct agaattggta ccaacgttat ctaattatct tggatatgta
     6841 caagagaagg aattgcttaa tgcaactagc ttagaaggta agaatattgc tgagttcgct
     6901 aaggataaga atatctatcg taagaataag gatttctttg aaagcacgtt tacgctatac
     6961 aacaaaataa ttgatacggc acaagaataa ggagtgattg aagtgaaaga aaatagattt
     7021 aaaaaattcg ctgaagctga agtagaagaa gttcctgaag tggttgaagc agttgaagaa
     7081 cctaaacaaa cacctaaaga aacaacaagt tctttggtag ctaaactaaa aagtgatagt
     7141 tctgaaaaga agaaaaatta tcaattcacg cttcaaccat cacaaagaaa gaaattatct
     7201 aaaatcagcg aaaagttagg aattcgatca gattcagctt tacttggtga aatgatagat
     7261 aacttgtatg atgaagtttt tggtgagtga tcagtttaaa gctgatcact ttttttattg
     7321 gaataagcgg ttaggtatag cttaactaaa tttcgtatac gtgtttatgg atatatacgt
     7381 atacatgttt atatacatgt atacgtatat gtttatgttt gtatttgtac ataataaaaa
     7441 ggctaggtat tgtaacctcg cctttttatt tttaaagtaa gtctttgtta cttggtgcat
     7501 tgaacccttt tagcattggc ttttctttct ttgctgaatg atcggatgta accgttgaag
     7561 gatgctcttg ttgaataggg ttatgatcag ttggtttctt ctttacttct gctactttgc
     7621 tatcgtttct atcgttgcct gcttcaataa ttgtaacttc attgttcttt acacgccgat
     7681 ataggttcaa agcttttttg atcgtctgag agtatttaac gcctagtgtt tcatattcgg
     7741 attcaagaaa ctctgctaaa ggtaactcgc taaccttttc tctattgatt ataactacat
     7801 aacgctctga ttttccctta ttcatgttga tctccttcgc tgttttattg agttaaacca
     7861 aacttgtaaa agccttttac attagaaaat tcactgtctt caacaaacaa ggtatgcgga
     7921 attttatctt ttacaagttg tttgtcgata atagaagctc caccaccagt aaagacaact
     7981 ttatctaagg ttgtgaagtt tttgaatgta gctaaaactt ctgcaacaac catattggtg
     8041 tatgttttta tttctctttc aactacttct gaaatatcaa aactttcatt tccgcttggc
     8101 ttaaatagat atacgccttt ttctttgcct tctcggataa tcttttcaag ttggaaagca
     8161 ttaggactga tcggaaggtc aagcatattt acgatacgat tatataaagt aaaaatgccg
     8221 gtatcctttt gttcacgatt gttttcgtca atttcaaaat tttggacaac atcaaaaagt
     8281 ttcgtgccac caccaatatc aacaaaaccg atcttctgac tgatcaatgt ttcatcttgt
     8341 acactaccat catcagcaag taggaggtta tatagtgttc caatcggctg tggcagtacc
     8401 caaacggtat ctacgtttat attataagcc ttaccgtcaa cttcaactga atgttgtcgt
     8461 gtggcttgtt gcatcacgta tttgacgatt gtttcggtat agtcggatga aggcagacct
     8521 aaaactagat taacggttaa aacgtcatca gattgatctt ctaaacgtgc taggttagct
     8581 aaagagaaag ataggatatt tttgtaggtt tcctgcttgt atctgctgat cccaaaacct
     8641 agtgattcta tcacgtagtc atcttttcct agctcgtgta tatccttccc aaaataatag
     8701 ctttcagtag tgtttactag gctatattca gaaacgttta gtgatcgttt taatgaggaa
     8761 cctatattag aaaaacgtcc tgaattaatg atatttttgt tcaaaatacg agagggaagt
     8821 gagagtggag aaacgtgtgt actcatttct tttactgatt tattccctaa gtcaacggaa
     8881 aacaataatg gttctttcat ggtatgacct ctttctgttt gtgtttatgg tatttcggta
     8941 cgaaaatagt ttacacctat ttactattag ttgcaactgt ttataagaaa aaaatgcaat
     9001 cata
//
LOCUS       DASJUF010000104         8979 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.105, whole genome shotgun sequence.
ACCESSION   DASJUF010000104 DASJUF010000000
VERSION     DASJUF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.105"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..74
                     /locus_tag="U4B62_002306"
     tRNA            2..74
                     /locus_tag="U4B62_002306"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="U4B62_002307"
     tRNA            78..150
                     /locus_tag="U4B62_002307"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="U4B62_002308"
     tRNA            155..225
                     /locus_tag="U4B62_002308"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="U4B62_002309"
     tRNA            238..311
                     /locus_tag="U4B62_002309"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="U4B62_002310"
     tRNA            323..394
                     /locus_tag="U4B62_002310"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="U4B62_002311"
     CDS             660..1853
                     /locus_tag="U4B62_002311"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN2142443.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="U4B62_002312"
     CDS             1983..3455
                     /locus_tag="U4B62_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2142444.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="U4B62_002313"
     CDS             3538..4326
                     /locus_tag="U4B62_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN2142445.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="U4B62_002314"
     CDS             complement(4393..5061)
                     /locus_tag="U4B62_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN2142446.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="U4B62_002315"
     CDS             5176..6888
                     /locus_tag="U4B62_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142447.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="U4B62_002316"
     CDS             6891..8663
                     /locus_tag="U4B62_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142448.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>8979)
                     /locus_tag="U4B62_002317"
     CDS             complement(8745..>8979)
                     /locus_tag="U4B62_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2142449.1"
                     /translation="YNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDR
                     VGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2693 a         1517 c         1986 g         2783 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatat
//
LOCUS       DASJUF010000105         8917 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJUF010000105 DASJUF010000000
VERSION     DASJUF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.106"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            42..2651
                     /gene="clpB"
                     /locus_tag="U4B62_002318"
     CDS             42..2651
                     /gene="clpB"
                     /locus_tag="U4B62_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN2142450.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2802..3488)
                     /locus_tag="U4B62_002319"
     CDS             complement(2802..3488)
                     /locus_tag="U4B62_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142451.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3466..3960)
                     /locus_tag="U4B62_002320"
     CDS             complement(3466..3960)
                     /locus_tag="U4B62_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2142452.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(3980..5200)
                     /locus_tag="U4B62_002321"
     CDS             complement(3980..5200)
                     /locus_tag="U4B62_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2142453.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5347..7182
                     /gene="lepA"
                     /locus_tag="U4B62_002322"
     CDS             5347..7182
                     /gene="lepA"
                     /locus_tag="U4B62_002322"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN2142454.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7378..8289
                     /locus_tag="U4B62_002323"
     CDS             7378..8289
                     /locus_tag="U4B62_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142455.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         3017 a         1574 c         1821 g         2505 t
ORIGIN      
        1 gaaataagtg atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga
       61 ctacgacgct tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc
      121 aagatatcga cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa
      181 atttctatac ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac
      241 tggatgagta tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga
      301 atttctttcg attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc
      361 tttcaacgga agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt
      421 atttgaataa aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag
      481 gaggagaaag agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg
      541 gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg
      601 aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga
      661 taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac
      721 gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat
      781 tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag
      841 aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag
      901 gggcaggaaa aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc
      961 gcggtgaatt acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa
     1021 aagacaaagc gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag
     1081 atacgatcag tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca
     1141 tccatgacaa cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt
     1201 tcttaccgga caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa
     1261 tgaactctat gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg
     1321 aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc
     1381 aagaagaatt agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag
     1441 aaaaagaaga agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg
     1501 aattagaaga tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta
     1561 cgatacctca attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga
     1621 ttaagatggt tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa
     1681 cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg
     1741 aaacacttca taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg
     1801 taattcgttc acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc
     1861 taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg
     1921 actcagaaga tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt
     1981 ctcgtctagt cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg
     2041 aagctgtcag acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc
     2101 ccgatgtctt caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag
     2161 ggcgagtagt agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt
     2221 tactgctgga aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca
     2281 acacgctatt acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt
     2341 tatttacacc attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat
     2401 tggcacaacg tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt
     2461 ggattgctga aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca
     2521 ctaaggaagt agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat
     2581 caaaagtaac gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag
     2641 aaatcgtcta atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt
     2701 cagacctcct tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg
     2761 aaatttcttg aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt
     2821 tctaatttat aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat
     2881 cccattaagg cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct
     2941 gctcgtccga caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag
     3001 aactggtcgc ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca
     3061 aagccgaata agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag
     3121 taaccaatca atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt
     3181 ttgtcccaaa aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg
     3241 aacaagaaaa accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc
     3301 aaaatgactt gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg
     3361 attccttgag aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg
     3421 aatacaccaa aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt
     3481 ccctccatct aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt
     3541 tccgttcaat ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc
     3601 tgtttcttgt agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt
     3661 atataagccg atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc
     3721 ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc
     3781 tgtgtcagca agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc
     3841 attttcttga ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa
     3901 ctgttggtta tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat
     3961 ctaaacatca tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga
     4021 tacgctctag tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat
     4081 aagaagcacg gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg
     4141 attttcctat aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga
     4201 agacaccatt attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg
     4261 ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat
     4321 atggttcgat ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt
     4381 tataaatatc tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata
     4441 agccatttcg agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag
     4501 ctacccagta gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg
     4561 tgcgctcatc ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata
     4621 aagcaccatg gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa
     4681 ctggtaatcg ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga
     4741 ttttaaagga aagtttatca gacatgattt cccatccttt acgccgatag taggggatac
     4801 tgtaaggata caaataagag atccattgct tattcttgcg catattatcc aatgcaacga
     4861 taattagatc ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca
     4921 caccacccat tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt
     4981 gagagatcag tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt
     5041 ttttggaacg gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag
     5101 tcacttggaa cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg
     5161 gttttaattc aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta
     5221 gcttgataac agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa
     5281 ctctttaaat tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga
     5341 gacgctatgg atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc
     5401 atcgctcata tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac
     5461 actgtgacgt ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa
     5521 cgtggaatca cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa
     5581 acatatatct ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct
     5641 cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa
     5701 gcacaaacat tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc
     5761 atcaataaaa tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac
     5821 gtaatcggaa tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt
     5881 gaagatattc tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca
     5941 ccgctaaaag ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat
     6001 gtccggatca cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc
     6061 aaaacatttg atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt
     6121 ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga
     6181 gtaggggata cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga
     6241 aaaatgaatc cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat
     6301 ttgcgggaag ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa
     6361 acctctcaag cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat
     6421 gtcgtccaag aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc
     6481 gttatttacc atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat
     6541 tttcctgaac ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg
     6601 gtcccaaatg attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc
     6661 attacgatgg attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct
     6721 gaaattgtct ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg
     6781 gattatgaaa tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat
     6841 ggagaaaaag tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga
     6901 aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc
     6961 caagcagcta ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac
     7021 gtcttggcta aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa
     7081 aaagaaggga agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc
     7141 atggccgttc tgaaaatgga tgagcaagat agtgtgaaat aacttataat caatcgaaaa
     7201 acctattaaa taaacctttt tgagtagatg caacactgag gattatctgt ataaaagatt
     7261 ttaaactgtc atttggttat cacggcgata ctaaaattaa caaaattgga atatttttct
     7321 tgaagatttt tagctgtcag tatatgatga aagcatctca taggaggtgc ttttttcatg
     7381 gttgataatt caagtggaag ggttcttaaa tctctaagaa aagagaaaaa actatctcaa
     7441 aaaaagttag ctgatttagc aggtatttca caaagtactt tagttaaata tgaaaaaggg
     7501 tcaagaaaaa tccctaaaga cgtagataat actctaagca agattctgaa tatagaaact
     7561 ctaataaaag atgaggaaga taagattgag attctcatcg gtaaactaat tgcatacaga
     7621 gatatgaata agttacttaa caaagagtta gctgataata taggaattag tgaagtatta
     7681 ctcagttatg tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat
     7741 atttttctat taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata
     7801 tttagtttat caaaagacga taaaattgtt atgggaaaaa gaatacgaga agtccgaaag
     7861 aaccgggaag agacgcttga aaaatttggt aaaaacttta ccatctatac aggcaaaaat
     7921 gttataagta gatgggaaaa aggtattaat attccagata ttgaaaaact aatgaatatt
     7981 gcctatcttg gtaaggtcac tgttccttac cttatgtacg gagaagatta taagaatata
     8041 cttccaaaag atgaaagagt tagcgatttt aaaaagataa attcttttag tatgggatta
     8101 aggatgagga aaattaggaa agattattat ctagaaaggg aggagtttgg aaagcttttt
     8161 tctccttcaa tttcaaaatg gtccatagat aggtatgaga atggtagaga tatacccaat
     8221 actaatcgaa ttatccaata tgcttatata ggaaatcttt ctttagaatt tttgatttac
     8281 gggatataaa aatagtttac gtcgatactt attaagttat cgacgttttt tattatagaa
     8341 aaaatttata aaaggaggtt tcttgatatg ataagaaaca aaaagggttt gttgaaagtt
     8401 gctttactgg cagctttttt ctaagtaact ttttgacaag ctcaatggca acacaagcgg
     8461 aagaagtaat acaagaacta actgttatga ataacagcta gataaaatgg gctatgtatg
     8521 ttatccaaca atttagtaat cactagaagc ctagcgaaag ttcgcttgat aagttataga
     8581 tgagtattga tttgtagtgt ttccaaatca aaagggtaca gagccacatg aaatatgttg
     8641 tcctctgcag cgtcaggatg ggaatccata ccatggagat tcggagatct gtgagggaat
     8701 acttacgtgg ttaatatgtc agttgtgttg gacgtagtaa tggtcatagt gggtagattg
     8761 actgaattct tatatgagaa aatgaaacca acaagaagat agggttattt atacagaatt
     8821 ggggatcttt tctggaaaag taatcggata atgcgagatt cgtacggtct tttttaaatt
     8881 tctctaaaaa aaacggcatc gaatgggttg ggtgatt
//
LOCUS       DASJUF010000106         8747 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJUF010000106 DASJUF010000000
VERSION     DASJUF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.107"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            139..648
                     /locus_tag="U4B62_002324"
     CDS             139..648
                     /locus_tag="U4B62_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2142456.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            741..1903
                     /locus_tag="U4B62_002325"
     CDS             join(741..1017,1017..1903)
                     /locus_tag="U4B62_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2142457.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2183..3163
                     /locus_tag="U4B62_002326"
     CDS             2183..3163
                     /locus_tag="U4B62_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2142458.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3285..4703
                     /locus_tag="U4B62_002327"
     CDS             3285..4703
                     /locus_tag="U4B62_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEN2142459.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4774..6243
                     /locus_tag="U4B62_002328"
     CDS             4774..6243
                     /locus_tag="U4B62_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2142460.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6253..6720
                     /locus_tag="U4B62_002329"
     CDS             6253..6720
                     /locus_tag="U4B62_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2142461.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6756..7628
                     /locus_tag="U4B62_002330"
     CDS             6756..7628
                     /locus_tag="U4B62_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2142462.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7710..>8747
                     /locus_tag="U4B62_002331"
     CDS             7710..>8747
                     /locus_tag="U4B62_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2142463.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMF"
BASE COUNT         3015 a         1305 c         1770 g         2657 t
ORIGIN      
        1 cacatgtaat cattgataag tccagtgttt tagcggataa tcagcttttt atttattttt
       61 tcttataaat ttgataaata gaaattagta tagccctaaa caaattattt gtgttttatt
      121 gtttagggct atactatatt ggtacaagga atagaaagag gtgtttatgt ggttgataaa
      181 attcagttta atcaagttat agaagaattc atcaaagtaa ataatttaat gacccatatt
      241 caaaaacgac caatcagaat aactaatgat ctaaaactca gtacgagtat gattcatttg
      301 attgatgtga ttggaaatta tcctgaaact agtattacgg aattagctga tcgattaggc
      361 gttaccaaag gagctgtatc ccaacagatt cccactttag agaaaatgaa tcttataagc
      421 atttctcaaa aaaaaggaaa taagaaaact aaacttgtat cgttaacaaa atatggtcga
      481 aaggtgtacg aatcacataa ttatttacat gaagagcttt atagctcaat aaatgaagcg
      541 ttagaatcat tctcaccaga ccaaatttca accattaaac agattctaga aaaagtatct
      601 tatacaatca atgaatatca agtaagtctg acaagaaagg aagattgaca tgaaaaaaat
      661 gatttttgaa tcggctacac tagactagac agaaaaaata aggtgtgtaa aataaatgaa
      721 aacacactgg aggaattttt atgtcaagac gtcaacgtag aacctattca aaagaattca
      781 aacaacaagt cgtcgatctt tatctcgcag gaaagccccg agctgaaatc attcgagatt
      841 atgagctgac tacctcggcc tttgataagt gggtcaagca agcgcaaact actggctcgt
      901 ttcaggaaaa agataacttg acccctgaac aagtagaact aattgcatta cgaaagaaaa
      961 ataaacagct tgaaatggag aatgacattt taaagcaagc ggcgctgata ttgggacgaa
     1021 aagacaagta attgatacca ataagcacaa atactcaata tcagcgatgt gtaaagtgtt
     1081 aaacatctct tgccaaacct attattatca agccaaacca gttgaagatg aatcagaact
     1141 ggaagaactc gttcaagaag aatttattcg caatcgaaaa gcttatggta ccaggaagct
     1201 gaagcagtgc ttggccaatc taggtcttca agtgagtcaa cgcagaatcg gccgtatgat
     1261 gaaacagcgt ggcttgcgtt caacatacac attggctcat ttcaaattcc attcttctac
     1321 ttgtaacgaa gccaagaccg ccaatattct agacggaacc ttcctacagg aaaagtcatt
     1381 agaagcgatt gtaaccgacc ttacctatgt tcgtgttggg aagaaatggc agtatatctg
     1441 cctgattctt gatttgttta atcgagaaat catcggctat tcatgcgggg agaagaaaga
     1501 tgcgacgtta gtcaaggaag cttttgcaaa gattccgtac tcgttgaata atgtacaact
     1561 ttttcacacg gatcgtggaa aagaattcga taaccagacg attgacgaaa ttttatgggc
     1621 ctttaatatc acgcgttcct taagcaaaaa aggctgcccc tatgataacg ccgttgcaga
     1681 atccacctat aaatctgtca aagtagaatt cgttcatcaa taccgctttg agacattggc
     1741 taatttgcga ttagagctat ttgattacgt ccattggtgg aataccatcc ggctgcacgg
     1801 cacgttaggc tatgagacac cgatcagttt tcgacagcag agattggcgc agcgaatcct
     1861 tgataatgag ctcggatgtg ataccggtgg cgaggcacct taattatttg aatgagcttc
     1921 tgccttagag aatcagattc gaggaggctc gttgtcaagg gcagtcggaa caccagcagc
     1981 aagcttacgc aataccttat aatttttgtc aaaaaaagtg ttgccattcc acatatatct
     2041 ttaaacagct attgccagat aaaaataaat gatattatat atataaatac ttatccgaaa
     2101 aggggaatga gagagtcaag gttcttggaa tgtcgaatag tcgagtaggt agattttcta
     2161 tctacaacct tgttgtttaa aaatgaaaaa aattggaatt agagaagttg cgaaagaggc
     2221 aaatgtttct cctacgactg tgtcgagggt attgaatgat cgtggatatt tgagtgagga
     2281 aacaaaaaga aaagtttttg atgccatgaa aaaattagaa tactatccta atgaactggc
     2341 gagagcactt tttaaaaacg aaacatatac cgtaggtttg atttttccta caattactaa
     2401 tccatttcac gctgagttaa ttcaagatat cgagttcgag ctttccagca ggggatataa
     2461 agtgttgctg tgcaatagct taaacaatcc aacaaaagaa aaagagtact tgacgatgtt
     2521 gagaaagaat caggttgatg gaattatagt aggaacgcac aataaagata ttgcggatta
     2581 tgatattcca gggctaccta ttgtagcgat tgatagaagt cttggtgaaa atacagttac
     2641 agtatcttgt gataattaca ggggtgggga attagctact caactacttg taaatcatgg
     2701 ctgtcagaat atactgtgta ttcggggaga tagtaagata aagctacccg cgaatagtag
     2761 gactttagca tataaagatg ttttgaataa attcatgctg gaaccgttag tcctagaagt
     2821 accatttgta aaagaaatca atgaaaaaaa tgaaataatt aagacatatc tggaaactca
     2881 tccaaatatt gacggggttt ttgcaggaga tgatttattg gcttctttgg cgattaacaa
     2941 cttaggaagt tcgggcaaaa gagttccaaa agaggttaaa gtgattggat ttgatggagc
     3001 tcaacaaaca ttgatttata atccagaatt atcaactatt caacagccta tagatcaaat
     3061 atctaaagta gcagtaaaaa aattattgaa tatgattaag ggcgaaaagg agacggatga
     3121 actttcactt ccagtaaagc tcataaaaaa aaatacagca taagttaaat cggcgaaaaa
     3181 ttccttcgtc gatttaaaaa tatttttatg aaacgggttg acatatgaaa cgggttgaca
     3241 tatattagtt gtgaagataa aaaccttttc actggaggaa gaaaatggag aaattattaa
     3301 ttaaaagcaa taatcatcaa ctttactacc aacctaagga tgtttgggtc ggagatgtta
     3361 tgccttacgg gaaagaagga aaattcttta tttatcatca acgtgatact agaaatccag
     3421 ttccttttgg agaaccgttt ggctgggctt tagcaactac gaaagatttt gttcattttg
     3481 aagactttgg ggaatctctt ttaagagggg atgacaatga gagagaccag tttatctacg
     3541 ctggttctgt atttgaggca aatggaaagg ttcacgcatt ttatacaggg tataatcgtg
     3601 aattcttagc gcagggaaag acatctcaag tgttgcttca tgctgagagt gatgattttg
     3661 ttcattggga aaaatcagaa acagcattgg agttagaacc acaagaaggg tatgatattc
     3721 gaaactggag agatccattt gtcgtttggg atgatgaaaa agaagaatat ttgctaattt
     3781 taggggcgcg aaagggtcag gataaacatc tacaaaccgg acgtttagtg gaatttacat
     3841 caaaagattt aactaattgg gaattcaaag gtgatttttg ggctccggga ttgtatacca
     3901 tgtttgaaat gccagaactt ttcaagattg gtgactggtg gtatcttgtc tattcagaat
     3961 actcagatgg aaataaaata ctctatcgaa tgagtcaaag tttggaaggt ccttggatta
     4021 agcctaaaga tgaagctttt gatggacggg cttattatgc aggtagaaca gcttatgatg
     4081 gaaaacgtcg agtcctattt ggatgggtac caacaaaaga tcaagaaaat gataaaaaca
     4141 attatttatg gggaggtact ttggttcctc atgaagtcta tcaaaaagag gatttttcac
     4201 ttggggtaaa acccatagac gaagtgtgga atgcattcat aaatttacat gatattgccg
     4261 atgtagagat agaaacagca gacacaagag aagaagtaac gcttgtcaaa aatagtggaa
     4321 ctttgtttaa tttagaaaca acaatttcat ttgatgagaa tcttagtgac ttttcactga
     4381 gattatataa aaaccttgag acagatgaat cttacgaata tcgcttcaat ttagatgagg
     4441 ggcgtcttac atttgataaa aacccttgtt acccttggtt tcaatatatg gataaaggac
     4501 ttattagacc tatatcttta aagcctaacc aatcttataa actaaaagtg attgttgatg
     4561 acaatataat gacactttat attgatggaa tcgctcttaa cgctcgtgta catggttgtt
     4621 ttggtcaagc aattactatt acagcaacaa atggatcaat gactgcaata aataccaagt
     4681 tttcaaatga aataaagaaa taataaacgt caatcatgtg gcaggtctga aaatcaaaca
     4741 tgacctgtca catttaaaag ggaggataaa tgaatggact acaaaaaaat tgccgaagat
     4801 gtattggaaa acgttggagg aaaagagaat atctcaagcg ctacacactg cattactcgt
     4861 ttacgtttaa ttcttaaaga caaaagcaaa gcgaatatgg aaatgcttga ggaaatagaa
     4921 ggtgcaaaag gtgttctttt caatagtgga cagttacaag taattttcgg aacaggtata
     4981 attgaaaagg tttacaaaga gttcgttgaa ttgacaggag ctaaggaagt atccgtttct
     5041 gaaatgaaag acgaaggagt caataattca ggaaatagac ttcaacgagc ttttaaagtt
     5101 ttctcggata tctttattcc gatcattcca gcttttattg gggcagcaat ggttttagga
     5161 attcgttcgt tgattaccac tcctggaatg tttggaatgg atggctcatt cgctgatcac
     5221 aatgtattcc taaatgattt atccaaattt ctgggtatta ttgcaaccac atttgattat
     5281 ttaccagttt tagttatgta ttctgcaaca aaacgttttg gcggaaaccc tattttagga
     5341 ttacttgtag gatttgttat gattcatcca ggactagcca atcggaatga tttcgtcatg
     5401 ggaactataa agcctgatta ttggcattta tttggattaa gcattccagc agttgcgttt
     5461 caagggggtg tttttccagc aattttgact tcttggtttt tagcaaaagt tgagaaaatt
     5521 acaaataaat attctcctca agtacttgct tacattttag tcccaactgt gacaattgtt
     5581 cttgccaact ttgcactatt tttagtgttt ggtccattgg ggaattttat cggtaatgtt
     5641 ttaggtggaa tcattgattt cctatatatg aagatgggtg cctttggtgc ttttgttttt
     5701 gcaggcgcgt tacaaccatt agttgtaaca ggaacacaac atgctattca aggtattgag
     5761 gcaaacttag tcgcaacaac tggattcaat tatatccaac ctatttggtc agtttcaatt
     5821 attgcccaag gtggcggttg tattggtatg tatttactct ttaagaaaaa atctaaagat
     5881 agagatattg cgatttcaag ctttattcca acactggtag gtattactga accggctatc
     5941 tttgctgtta atttgaaata ttcaatcgtt ccttttgttt gctcgtttat tggtgctggt
     6001 tttggtggag catttatgaa aatttttgat gtaaaagcca ttggacaagg attgactgta
     6061 ttgccaggtc tgactattgc taatccagtt cttccttata tatttggtaa tctcatagca
     6121 ttcattttac caatcatttt tattctcgct tatgataaat ataaagtgtt tatccctgaa
     6181 gctaaacacc gtaaatttgc agaaaaaact gactcaaaaa aattgccagt tcaagtaaag
     6241 tgagatgaga gcatgttaaa tttttttaga aaaaatgaaa taagcgttgt agcacctgca
     6301 accggtgttt ttgaaaaact ggagaaatta agtgatccag tcttttcaaa aggaatgatg
     6361 ggacaaggat ttgcaattga tgcaaaaaac gaaacgattg tatctccaat cgatggtttt
     6421 gtttcaagta tttttcctac aaaacatgcc ttaggaataa aaacaaaatc aggaattgag
     6481 atacttgttc atgtaggaat agatacagtt gagctaaatg gtgagggatt tgatattaaa
     6541 gttcaagaag gacaaaaagt taaggctggg gatattctta tgaccattaa ttttgtagtt
     6601 tttgaaaaga atcacaagac taaagatgtc attgttgttt ttccagacta ccaagacgaa
     6661 attttcattc cgaaacttga agaagtaaaa caaggacaag aattgtttgt tcttggctaa
     6721 tgataagtaa ttaataatag aatcgaggaa aatgtatggt ttttggggct attgaagctg
     6781 gtggaacaaa atttgtttgt gccattggag ataaagattt gaacatattt gaaaaagtca
     6841 gctttcccac gactacaccc aaagaaacaa tgaatttagt tttagatttt tttaatcatc
     6901 ataaagatga tttagaaggt atcggagtgg gatcgttcgg tcctattgat attcattgtg
     6961 aatctaaaac gtatggcttc attacatcaa cgccaaaact tgcttggcag gattttgatt
     7021 ttattggaag tttaaaaaag caacttaata ttccaactgc ttggacgact gatgtaaatg
     7081 cctcttgcta tggtgaatat gtttctggaa atggcagagg cctttcaagt gttgtttact
     7141 ataccgtagg aacgggcata ggaggtggcg ctatccaaaa cggaaacttc attgaaggtt
     7201 ttagtcatcc agaaatggga catatgcttg taaaacgaca caataaagac aacttttctg
     7261 ggacatgtcc attccacgga gactgtttag agggcttagc tgctggtccg gcaatcgaaa
     7321 gaagatcagg gaaaaaggga caagaagtga ctgaaaatga cgttacttgg gaaatagaag
     7381 cttattacat tgctcagtgt gcatataata caacattgat gttatcacct gatgtcatta
     7441 tatttggtgg cggagtaatg aaacaaaaac acttaaaaaa gaaagtacaa gataagttta
     7501 ttgagttgat caacggatat gtcgctattc ccgatgttga caattatatt atgacaccaa
     7561 aattgttaga taattctggt attgtaggtt gtttagcttt agcaagagac gttaaagaaa
     7621 aatcttaatt tggaaaaaag atcgtctcga ctatttgttt ggttgatgcg atcttttttt
     7681 tcaattagta attgaaggga cgcctaaaag tgaaaaaaaa aatgaaagat gaagtattag
     7741 agtatatcaa aaataattta aagtattacg atttttctgc tcaagatatt gcaatgaaat
     7801 tttgtattaa aagaaatgtc gcttcccact atttgaatca gttattttca gatggaaaat
     7861 tactaaagaa tgattctgtt agaccagtaa tgttcaagta taatcaacaa aagccaaaag
     7921 actgcttttc taagtttatt ggagcagaca tatctttgaa atccactata gataaatgca
     7981 aagctactgt aatgtatccc cctaatggcc tcccattaat aataaaagga aacagtggcg
     8041 ttggaaaaag ttttttagca agtttgatct atcagtatgc tcttgataga aaagtaattc
     8101 ataatgacgc aaaatttgtt gttgtgaatt gtgcagatta tgcaaataat ccagagctat
     8161 tgtcagcagt tttatttgga tataaaaaag gagcctttac tggggctgaa aaagatactg
     8221 cagggatgct atcttctgct aatggaggat accttttttt agatgaggtt cacaatctaa
     8281 gtgcagaaaa ccaagagaaa ttgtttcttc ttatggattc tcaaaagtat cgtaaacttg
     8341 gtgaaagtgt gcaatgggag tatgcaaatg ttcgccttat cttagccaca acagaagata
     8401 aaaatacctc tttgttagca acttttagaa gacgcatccc agctgaaatc actctgccag
     8461 attacaataa tagaagtaca tctgaaaaaa ttcaattatt atttgaattt cttaaagatg
     8521 aagcaattac aattgattca aaaatatttt gttcgattga actttttact gatcttttat
     8581 cgaaaacatt tgaaggaaat gttggagaat tgaggaatga aatcaagctt ctttgtgcag
     8641 aaggttattt aggaaataag agaaagtctt caatttattt aggggacaaa ctagacagcg
     8701 gtttttggat agatccagaa attagtatag atttaaaaaa aatgttt
//
LOCUS       DASJUF010000107         8685 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJUF010000107 DASJUF010000000
VERSION     DASJUF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.108"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(49..495)
                     /locus_tag="U4B62_002332"
     CDS             complement(49..495)
                     /locus_tag="U4B62_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN2142464.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(522..698)
                     /locus_tag="U4B62_002333"
     CDS             complement(522..698)
                     /locus_tag="U4B62_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN2142465.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(860..1288)
                     /locus_tag="U4B62_002334"
     CDS             complement(860..1288)
                     /locus_tag="U4B62_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2142466.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1380..2240
                     /locus_tag="U4B62_002335"
     CDS             1380..2240
                     /locus_tag="U4B62_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN2142467.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(2334..2684)
                     /locus_tag="U4B62_002336"
     CDS             complement(2334..2684)
                     /locus_tag="U4B62_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN2142468.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2700..4427)
                     /locus_tag="U4B62_002337"
     CDS             complement(2700..4427)
                     /locus_tag="U4B62_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN2142469.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="U4B62_002338"
     CDS             complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="U4B62_002338"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN2142470.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5711..7147)
                     /locus_tag="U4B62_002339"
     CDS             complement(5711..7147)
                     /locus_tag="U4B62_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2142471.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7144..8547)
                     /locus_tag="U4B62_002340"
     CDS             complement(7144..8547)
                     /locus_tag="U4B62_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2142472.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         2346 a         1947 c         1492 g         2900 t
ORIGIN      
        1 gaatttcttc accaacactc attgacgaag agcctttaaa tgataaaatt aagattgtaa
       61 tagttctttc acaatggcat ttacttggtt tccatctgct tttcctttga ctttaggcat
      121 gatcgcaccc atgactttgc caaattcttt cattgatgaa gcaccagttt gtgtgattgc
      181 ttcttgaaca agttgtcgaa tttcttcgtc ggaaagttgt tccggcatgt atttttcgac
      241 tatcacaatt tcacttttta ctttttcagc taaatcgtca cgtcctgctt cttcaaactc
      301 atgcaaagaa tcacgtcgct gtttcatttc gcgagacaac accgttaatt cttcttcttc
      361 tgtcaagtcg tgaccttctt taatctgctc gttttgtata gatgacttga tcattcggat
      421 gacttgtaaa gattccttat ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct
      481 acttagtagt gacacactac caactccaat tcatcacaga actagaattt tttgcgtttt
      541 ctagctgctt cagatttttt cttgcgtctt acacttggtt tttcgtagaa ttcacgttta
      601 cgagattctt gtaaagtacc cgcttttgac acggaacgtt tgaagcgacg aagagcatcg
      661 tcaagtgatt cgtttttgcg aacgactgtt tttgacatgt taattccctc cctccgagct
      721 taaaaagtaa ccgtaattgt tattgaaata tatctcaaaa aacaagactg tcctgtaaat
      781 attatatcta atgctgtttt gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa
      841 tgttatttta tttaaatcct tattgacaat tttcacatct tccgaaaatc tcgaatctat
      901 gcccttcgat cgtacatcca ccgagctgtt cttcgaaata attcattgga cacatatgga
      961 tttctttcgt tgttccgcac accgtacaga taaagtgatg gtgatggccg atttcccctt
     1021 gacagcaatg aaaacgaaac ttcatctctc catttaaatc ggtttcttcc agtaaatcga
     1081 tttctgcaaa atcatggaga tttcgataaa tcgtatcgta acttactcct ttgaattcct
     1141 gattcatata gtcaaaaact tctttagcgg atacgtagcg attgcgcttc actaaataag
     1201 ttaacaatgc ttctcttttt ttagtatatt taaaaccgtt atttttcaat gtttctagtg
     1261 attgttcaat caatgtctgt tttttcatga cttattcctc caaatcaaaa tgattacgaa
     1321 gtacacattc tagagtatgg tataataaac ataaaaaaaa tgcaagtgag gttttatcca
     1381 tgtcttccga ttctacagaa atcattactt tttttgtcat ctctgactct gctggagaaa
     1441 ctgcgacaaa attagcgcaa gccactatgg cccaatatcc taccgttgag ttcaatttat
     1501 tccgcagaac atttgtgaca gatgagaaaa cattgatgca ggcactgcaa gatgcactaa
     1561 aagaaaaagc ccttgtccta cacacattga tcaatcaaga attgatcgag atgacgcgtt
     1621 ctttttgtga agaacatcaa ttatttagtt ttgatgtgat gactccgccg atatcagaaa
     1681 tcgagcgctt aactggtatc aagcccattc ggcaacctgg cgcccttcat ttattgaatg
     1741 agaactattt caaacggatc aaagcaatgg aatttgctgt aaaatacgat gacgggaaag
     1801 atcctcgtgg gtttctagaa gcagatgtcg ttttgctagg cgtatccaga acttccaaaa
     1861 cgcctttgag cttgttttta gctaacaaaa atttaaaggt tgccaatcta cctttgatcc
     1921 cacaagcaca tattcccaaa cagctttggg agatcgatcc taaaaaaatc gtgggactga
     1981 cgaataaccc agatatttta aacaatatcc gtaaagaacg gatgcgctct tacggattaa
     2041 atcctgatac tgcttattcc gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg
     2101 atctttatga aaaattgggc tgcgtagtaa tcaatgtcgc ttctttatct atcgaagaaa
     2161 cagcttcact tattttgaat gctttagacc tagaagatca tagttattat ggaactgaaa
     2221 ccaccgaaga aacagactaa cagtttttct tgtctttttc ttaaaaaaac tggactgtgt
     2281 cagcgaagaa gattcgttga tacagtccag tttgtctcta tttgcaccta tcgttattga
     2341 tgttccacat gtttgagcgt ctggttcaaa atatccagta cgtgagaatc atcaagacta
     2401 tacaaaatcg cttttccctc acgatgggac ttgacgacat ggttttctct taataaacgc
     2461 agttgatgcg atacagcaga ttgttccatt tctactgctt gggtgattgc tgtcacattt
     2521 tgttccccgt ctttcagata cagcagaatt ttgagacgag tggggtcact caacattttg
     2581 aatagttggc tgactttctc tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc
     2641 aacttgaaaa aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt
     2701 tattcttctg tcattttttt gataatcgct tcataagcgg ctactttttc agctgcgtct
     2761 tctgaagagt ttccttttac accgatataa aatttgattt tcggctcagt tcctgaagga
     2821 cggatagcga tccagccatt ttcttccagc gtatacttca atacgtcaga tggcggtgtc
     2881 gttagttttt ctacgtttcc ttcacggtct gttctcgtca actctttgaa gtcttctgtt
     2941 tggatgactt ttgctccggc aaattctgtc ggtgcttctt cacggaattt ttccatcaaa
     3001 gcagagattt ttgcagagcc ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg
     3061 tagtagccat atgtttcaaa aatcgtttgc aagccgtcat acaatgtttt gccttgtttt
     3121 ttatagtaag ctgctacttc tgctaataag agcaatgctt gaatcgcgtc tttgtcgcga
     3181 acgaaaggtt taactaaata accgtaactt tcttcgaatc cgaacataaa agtatgggaa
     3241 tgatcttctt caaattgttg aattttttca gcaatgaatt tgaatcctgt caatacattg
     3301 aacatggtag ccccatagtg ttccgcaatt gctgtaggca attcgctaga aacgattgat
     3361 ttcaataccg ctgcattttc aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat
     3421 tgagcaaaca gggctccgat ttggtttcct gtcaatactt cataggagcc atttggcaaa
     3481 cgaacggctg cgcctaaacg gtctgcatca ggatccgttg caatcagtaa gtcagcgcct
     3541 tctttctcgc caagtcgcat cgcatactcg aaagcagagt gttcttctgg attcggtgat
     3601 ttgactgtag tgaaatccgg gtctgcgaca gcttgttctg gaactagtac gaattgttcg
     3661 aatccagctt gtttcaacgc acgttcgccc aacatttttc ctgttccgtg aagtggtgtg
     3721 tacacaagct tcaaatcttt acccattgtt tccaccaatt catgatcaat cgttactgct
     3781 ttgatttctt ctagataagc ggcatccact tcttctccga tgatcgtgat caatccgcta
     3841 tgttcagctt cgtcgtcaga taatacttca acttttaacg gattgctgac ttcacggaca
     3901 tatttcgtca atgcatctgc gtctgcagga ggcatttgtc ccccatcttc tccgtagact
     3961 ttatatccgt tgtaagctgc tggattgtgg gatgcagtta ccatgattcc cgcaaacgtc
     4021 ttcaaatgac ggactgcaaa agaaagctct ggtgttggac gtaaactttc gaatacataa
     4081 gaagggatat tgtgatgtgc taatgttttg gctgcttcca tggcaaattc tggtgattga
     4141 tggcgtgagt cataagcgat agcgacacct cggcgttttg tttcttcacc ttgtgtatcc
     4201 atgaaacgtg caagtccttc tgttgcttgt cttaccgtat agatgttcat tcgattgatc
     4261 ccaggtccta agattccccg catgcctgca gtaccgaatt ccaacggtgc ataaaaagca
     4321 tcttcaagtt tttcaggatc ttcacccaga tcatttaatt gtttttttaa gttttcttct
     4381 aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa aataacacct
     4441 ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg aaagtatgag
     4501 gaccaaaaaa cgcttaaatc aagaaaagat tcttttatca agcacggtta caagtattcg
     4561 cataaatcgg attcagtaga tttcacgcaa aaaaaagagt aaaagaacaa ttgaaaccgt
     4621 tctttcactc tttttaaact tatgcagttg tttcttctaa agattccaag tatttataca
     4681 cttgttgtcc agcaatccca ccatcaccaa cagcagtagt gatttgtcgc aaatcttttt
     4741 cacgaacatc tccgatggca aaaacgcctg gtatattcgt cttcatttcg ttgtctgttg
     4801 gaatccagcc ggcttcgttc gtcaacccag cttctctaaa tggtgcagtt aatggatcca
     4861 atcctacata gatgaatact ccgtctgctg ccaaatcaaa ctcattgcca gttttgacat
     4921 tacgtgcttt cacacctgtc actaccattt catttccaac gatttcgtca acgactgtat
     4981 cccacataaa ggaaattttt tcattcgcaa atgcacggtc ttggatgatc ttttgtgcac
     5041 gtaattcatc tctacggtgg acgatgacta cttcagaagc aaattgtgtc aaatagatgg
     5101 cttcttctac tgctgagtct cctcctccga tcactactag acgtttgtta cggaaaaatg
     5161 caccatcaca aactgcacag taagaaacgc cacgtccagc aaattcttcc tcgccaggta
     5221 cgcctaattt acggtggata cagcctgttg cgataatgac tgtttttcct tcgtaagttt
     5281 tatcttcaca aataacttct ttgtggctgc cgtgatcctt gatatcttgc acgattccat
     5341 aagcattttc tgtcccaaat cttgaaacac cttcatacat cttataagct agatcaggac
     5401 ccatgattga atcgaaacca ggatagtttt cgatttctgc cgtattgttc atttggccgc
     5461 caggagcgcc ttgttcgatc atcaatacgg ataagtttga acgtgaagca tataaagcag
     5521 cagtcatccc agcaggccct gcgccaataa cgattacatc atacatatgt agattctcct
     5581 ttacttaatc aagtaagcat actttacagc caatgcgaaa aaaagtctat tgttctgctt
     5641 gcgtttctcg aatagcttta ttatactcga aaaatccgct gaaaaacaat agacttttat
     5701 aaatcattga tcaatcgttc gaaagatttt ctccatttga atcaattact tttttatacc
     5761 agttgaatga tttcttcttc gaacgggcta atgtgccatt tccttcgttg tcacgatcga
     5821 cataaatgaa tccgtacctt ttcttcatct cacctgtacc agctgatact aaatcaatac
     5881 atccccaagt cgtatagcct aatagatcga ctccatctaa ttctactgca tctttcattg
     5941 cctggatatg tgctgccaga tagtcgattc gataatcatc ttccacataa ccattctcat
     6001 cgggcgtatc gactgcacct aagccgtttt ctacaataaa tagtggtttt tgataacgat
     6061 cataaatatc attcatcgtt attctcaagc ctaatggatc aatttgccaa ccccattcac
     6121 ttgcttccag atatggattt ttgacagaag caaagatatt tcctgcagtc tgttccaaga
     6181 gttcgttgtc agtagtcgaa actcgagaag aataataaga aaaggagata aaatcaacag
     6241 tatgttcttt cagcaatgcg gcatccccag gttccatttt gatctgaatg ccatttcgtt
     6301 ccatttcttt caatgcataa gcgggatatt ctcctcgtga ctggacatca atgaagaaat
     6361 aattttctcg atctgacttt ctagaagccc aaacatcttc aggattgcaa gtgtatggat
     6421 aatatgctcc tgctgccagc atacagccta ctttgttctc cggatctact tcatgagcga
     6481 tcttcgttgc gatggcactt gcaaccaact catgatgtgc cgcttgatat ttgatttgtt
     6541 cttcattttc tccttcttca aaatacagcc ctgcgcccat gaatggtgca tgaaggatca
     6601 tattgatctc attgaatgtc aaccaatatt tgacaagtcc tttatagcga ttgaagatga
     6661 ctcggcacag attttcataa aaaccaacga gttctctgga acgccacgca ccatattctt
     6721 tgatcaaatg catcggacaa tcaaaatgtg taatcgtcac aagcggctcg atcccgtact
     6781 tttgacattc tttaaagaca tcctcataaa atttcaaccc agattcattt ggctcttttt
     6841 catcgcccat tggaaaaata cgactccaag caatggacat ccgataagtc ttgaacccca
     6901 tttctgcaaa taaagcaata tcttccttat aatgatgata catatcgatc gcaatttttg
     6961 ctggataaaa atgattttca tcaaattcaa acattttctt ttttccagta atgacaggaa
     7021 aacggtcttc tccgattggc acaacatcta cgttcgctaa cccacgtccg ccttcatcat
     7081 aaccgccctc gcattgattg gctgctgtag ctccgcccca taaaaaatct tttctgaatc
     7141 ccatcatact tcctccatta catgtgtatt gcttgataat tctcttgcca gttcatcttg
     7201 gacttcgatt ccttctgctt ttaaccggct gattgtttcc ttgaattgcg gtaaatactg
     7261 ctgatgtgca ataaacaact catccatgat ccgttttgct gtctttcctg atcgtataag
     7321 cggattgatc gtaaacgctt gcaaagctgt tccataatca ccagtcacgg ctgcttcgat
     7381 cgtcagcaat tccatattct tcattacttg caaccagcct ctttcagcag caggaaggga
     7441 accaaacgct acatttcgtg cgccttgtgc gccaacatag gcagtgactt ctactacaca
     7501 atctgccggc agatcaggaa cagctccatc attctttgta gataccacga tctgtgtttc
     7561 tttatttcca tagatcgaag caatcgtttc acaggcagca tctgaataat aagcaccacc
     7621 tcgtttttgc aattgttcag gtttatgatc caattctgga tctttgtata attcaaacag
     7681 ctctgcttct gtttctttca cttgctgcgc tcttgtacca atcgtttgat attcttctaa
     7741 agcatgttcc agcatttctt cttcacgata gtaatagcgg tggtagccac aagggatcat
     7801 gttcatttgt tttaattgtt ctttgaaaaa cgggatgtca aagatatttt tcggcaatcc
     7861 attgtcttca tcgtataaac gatcaatcaa ttctgccgtg cggtcatttc ctttattatc
     7921 tgctactttg tgccaatgga agtgatttag accggcaaac ttgtagatca gctcttcttt
     7981 tttcttgcca agcattgttg gttctgtcat catcgccatt actggcacat tgcataatcc
     8041 gatcacttta tcccatttgc catatctgat aactgcttct gtcaccatcc cgcttgggtt
     8101 cgcgaaatta atcagccaag catttggaca taaacgcttc atgtcttcaa cgattgccaa
     8161 aaccactgga acggtacgaa aagctttgaa tatcccgcca gcaccatttg tttcttgccc
     8221 aatcatccca taataactag ggatccgctc gtctttgatt cgtgcatcta attgaccgac
     8281 acgaaactga gtcgtaacaa aatccgcatc tttcaatgct tcttcccgat ccagagttgc
     8341 atagattttg acttcatacg gagaagcatc ccacatacgc tgagacatct gactgacgat
     8401 ctcaagcttt tcttttcctt cttctacatc tacgagccag atttctcgta tcggtaattc
     8461 ctcatatctt ttgataaatc cttccatcaa ctcgggggtg taactgctgc ctccaccaat
     8521 cgtagcgatt ttgattcctt ttgccatgtt ctcactcctt tactttttac acttttatta
     8581 tcttttttgt aaacaaaaca aacaatagaa attcgtttct ttccagtaac atttacaaaa
     8641 gttagtgttt tcttctggat ttttgtaaat atcttgtcaa aaaat
//
LOCUS       DASJUF010000108         8646 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJUF010000108 DASJUF010000000
VERSION     DASJUF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.109"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(248..2629)
                     /locus_tag="U4B62_002341"
     CDS             complement(248..2629)
                     /locus_tag="U4B62_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2142473.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2619..3266)
                     /gene="recU"
                     /locus_tag="U4B62_002342"
     CDS             complement(2619..3266)
                     /gene="recU"
                     /locus_tag="U4B62_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN2142474.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3330..3869
                     /locus_tag="U4B62_002343"
     CDS             3330..3869
                     /locus_tag="U4B62_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN2142475.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3959..4378
                     /gene="gpsB"
                     /locus_tag="U4B62_002344"
     CDS             3959..4378
                     /gene="gpsB"
                     /locus_tag="U4B62_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN2142476.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4400..4767
                     /gene="rnpB"
                     /locus_tag="U4B62_002345"
     ncRNA           4400..4767
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U4B62_002345"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4976..6142
                     /locus_tag="U4B62_002346"
     CDS             4976..6142
                     /locus_tag="U4B62_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN2142477.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6188..7684
                     /locus_tag="U4B62_002347"
     CDS             6188..7684
                     /locus_tag="U4B62_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN2142478.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7765..8448
                     /locus_tag="U4B62_002348"
     CDS             7765..8448
                     /locus_tag="U4B62_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2142479.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2719 a         1570 c         1836 g         2521 t
ORIGIN      
        1 actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta aatggataaa
       61 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
      121 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      181 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      241 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      301 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      361 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      421 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      481 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      541 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      601 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      661 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      721 tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      781 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      841 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      901 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      961 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
     1021 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1081 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1141 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1201 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1261 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1321 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1381 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1441 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1501 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1561 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1621 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1681 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1741 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1801 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1861 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1921 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1981 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     2041 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2101 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2161 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2221 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2281 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2341 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2401 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2461 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2521 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2581 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2641 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2701 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2761 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2821 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2881 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2941 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     3001 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3061 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3121 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3181 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3241 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3301 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3361 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3421 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3481 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3541 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3601 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3661 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3721 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3781 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3841 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3901 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3961 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     4021 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     4081 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4141 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4201 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4261 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4321 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4381 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4441 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4501 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4561 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4621 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4681 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4741 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4801 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4861 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4921 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4981 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     5041 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5101 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5161 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5221 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5281 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5341 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5401 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5461 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5521 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5581 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5641 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5701 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5761 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5821 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5881 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5941 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     6001 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6061 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6121 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6181 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6241 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6301 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6361 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6421 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6481 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6541 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6601 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6661 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6721 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6781 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6841 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6901 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6961 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     7021 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7081 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7141 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     7201 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7261 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7321 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7381 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7441 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7501 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7561 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7621 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7681 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7741 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7801 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7861 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7921 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7981 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     8041 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     8101 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     8161 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     8221 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8281 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8341 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8401 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8461 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8521 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8581 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8641 accgtt
//
LOCUS       DASJUF010000109         8494 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.110, whole genome shotgun sequence.
ACCESSION   DASJUF010000109 DASJUF010000000
VERSION     DASJUF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.110"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="U4B62_002349"
     CDS             complement(<1..173)
                     /locus_tag="U4B62_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2142480.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U4B62_002350"
     CDS             complement(593..1714)
                     /locus_tag="U4B62_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEN2142481.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U4B62_002351"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U4B62_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEN2142482.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U4B62_002352"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U4B62_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEN2142483.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U4B62_002353"
     CDS             complement(4304..5215)
                     /locus_tag="U4B62_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2142484.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U4B62_002354"
     CDS             complement(5232..6239)
                     /locus_tag="U4B62_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN2142485.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U4B62_002355"
     CDS             complement(6236..8365)
                     /locus_tag="U4B62_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2142486.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="U4B62_002356"
     CDS             complement(8370..>8494)
                     /locus_tag="U4B62_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2142487.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DASJUF010000110         8252 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJUF010000110 DASJUF010000000
VERSION     DASJUF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.111"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            328..597
                     /gene="rpsN"
                     /locus_tag="U4B62_002357"
     CDS             328..597
                     /gene="rpsN"
                     /locus_tag="U4B62_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2142488.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            612..791
                     /gene="rpmF"
                     /locus_tag="U4B62_002358"
     CDS             612..791
                     /gene="rpmF"
                     /locus_tag="U4B62_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2142489.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            823..1200
                     /locus_tag="U4B62_002359"
     CDS             823..1200
                     /locus_tag="U4B62_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142490.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1221..1370
                     /gene="rpmG"
                     /locus_tag="U4B62_002360"
     CDS             1221..1370
                     /gene="rpmG"
                     /locus_tag="U4B62_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2142491.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1394..2377
                     /locus_tag="U4B62_002361"
     CDS             1394..2377
                     /locus_tag="U4B62_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN2142492.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2407..2529
                     /locus_tag="U4B62_002362"
     CDS             2407..2529
                     /locus_tag="U4B62_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2142493.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2547..4070
                     /locus_tag="U4B62_002363"
     CDS             2547..4070
                     /locus_tag="U4B62_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN2142494.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4094..4333
                     /locus_tag="U4B62_002364"
     CDS             4094..4333
                     /locus_tag="U4B62_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142495.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4526..4987)
                     /locus_tag="U4B62_002365"
     CDS             complement(4526..4987)
                     /locus_tag="U4B62_002365"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2142496.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5182..5400)
                     /locus_tag="U4B62_002366"
     CDS             complement(5182..5400)
                     /locus_tag="U4B62_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142497.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5518..7134
                     /locus_tag="U4B62_002367"
     CDS             5518..7134
                     /locus_tag="U4B62_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2142498.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7375..>8252)
                     /locus_tag="U4B62_002368"
     CDS             complement(7375..>8252)
                     /locus_tag="U4B62_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2142499.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT         2823 a         1273 c         1651 g         2505 t
ORIGIN      
        1 acataaaaga ttgaataaaa agttatagtt tctttcatat tgccgtttta agtagaggaa
       61 aagctactta aaacggtatt tgtgtttgat tggactaata aaagtgaatt cttatttttc
      121 agagcctaaa ctgcatattc ttttaaatga tttgattatc tctatgtacc gataaattcg
      181 ttttcaaata gttatctatt catatgagtt ttatttcatg ttatttattg ttgacaagag
      241 caactcttgt tggtaaagta tcagtgtcaa aacgtaacga ttacgatttg aggtgtctaa
      301 atcaaaataa aaggagtata aaggtatatg gcaaaaaagt cgaaaattgc taaagcaaag
      361 aaacaaatgg cgatgattga aaagtatgca gataaacgtc aggaattaaa agccgcagga
      421 gatcgcaccg ctctcgctaa attgccaaga gattcaaatc cgaatcgtct gaggcttcgt
      481 gatcagacgg atggtcgtcc aagagggtat atgcgaaagt tcggtatgtc ccgtattaag
      541 tttagagaac ttgcccatca agggttgatt cctggagtaa aaaaagcaag ttggtaaaag
      601 gaggaaatat catggcagta ccagcaagaa aaacatctaa ggcgaagaaa cggttaagaa
      661 gaactcatca aactgtagta aaaccagaaa tttcatttga cgaagcgaat ggtgattata
      721 gacgtagcca tcatgtatct ttaaaagggt actataatgg aaagaaagta attaaaggag
      781 cttcaaaata gtttaatata taacttttag gagggaatca aaatggaaat tatttaccca
      841 cctttagttg aagaaggatt aaagtattat cttgaaacga cccagcaatc gctagataaa
      901 tgcacttttt atcgttcgat ggtagagaga ggaattatta ctgaaacggg tttaccgacc
      961 caacaggcaa ttgaaaatgg attagtcaaa gattactatg aggaccaagg cttatcattt
     1021 gatgattttt taagaatcta tccaattttt gaagagtatg acgaggagtt atttcaatgt
     1081 atcgatggtt attgggagat acctgttgat atgaaagaaa atttagtttc acaattggaa
     1141 tctggcgagt taacttttga agatgcacaa caaattcaag catatcttga agatcgataa
     1201 gtattgaagg aggaagattt atgcgtcaaa atattatttt ggaatgtgtc gaaactggag
     1261 aacgtctgta tctcaccagc aaaaataaac ggaataaccc tgaacggtta gaattaaaaa
     1321 agtattcgcc aaaattgcgc agacgggcaa tttttaaaga agtgaaatag ctaagtggta
     1381 aaaaggtgac aaaatgggca taccagttac aatcgtttct gggtttttag gggctggaaa
     1441 aacaacgcta atcaatcaag ccttgcaagt ctcaccctac cccagggaag aaattattat
     1501 aattgaaaat gagtttggtg aagttgggat tgatcacaaa ttattagttc atagcgagga
     1561 acgggtgtta caactaaata atggatgtat gtgttgcagt cttcgcagtg atttattagc
     1621 aatattgatt tctttactgg aaacaataga agaacatcaa caaccaattt cacaaatcat
     1681 cattgaaact acaggtatag cagatcccca accaatcatt caaacacttt taacagcgcc
     1741 tcaactaaga ggaagagtat atatcgatag tttattgact gttattgata gtgacagttt
     1801 tgaacttatt gaaagagaac cacaagccct taagcaactt gtcatggcag atcgcatttt
     1861 tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa aaattacaga gagaaatcaa
     1921 aagtattaac cctttatccg atattcgaat gttcacacat agtgaaacga ttcaaaaaaa
     1981 ggatttttat aatttgaaaa aatttaatca tcccttagtt tttgaaagtg attcatcaga
     2041 aaatcactat aatggcaaat actatcatca acaccatcaa gtacatgagt ttaaatcaat
     2101 tctcttaact tcagatcaag acataaaaaa agatttttta ctccaatgga ttgactggct
     2161 gattttaacc aaccaaggga gcctgtatcg ttttaaagga ttgattgctt tacaagagca
     2221 ggattttata gttgccatgc aagaggtaaa cgaacaggtt aattttcaat acaccactcg
     2281 acaaaaagac caaaccacta ttattctaat cggaaaaatg ttggatgaag aaaggatcaa
     2341 acagagtttt gataaattac tggcggaaag caaataatga taagtagtga ttgggggtaa
     2401 tttctaatgg agaaaacgga tatatctagt gcatatcgac gacttaaaag cccgaatatt
     2461 aaaactagaa aaagggcatt aaaaattatc aaagaacata aacgaaataa aatgaagaaa
     2521 ttagcataat taaggaagga aaaagaatga agaaaatcag tgtaggactt attgggatag
     2581 cagctctagg attattggga gcttgctcca gcactaatga tgcaaaagta tcgaatgata
     2641 aagatggaaa attagaaatt gtaacaacat tctatccaat gtatgatttc accaaaaaca
     2701 tcgtcggcga tgaagcgaac gtggatttaa tggttccagc gggatcagaa cctcatgact
     2761 atgagccctc agctaaggat atggcaaaag ctcatgatgc tgatgttttt gtatatcata
     2821 acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag ttggaagaaa gctgggccta
     2881 atgtagtaga aggaacaaaa gacatgattt tattgccagg tagtgaagaa gaagatcatg
     2941 atcacggaga agaagatcac catcatgaac tagatcctca tacgtgggtt tcacccaaaa
     3001 tggcaattaa ggaagtttca aatatcaagg atcagttagt taaattgtat ccgaaaaaag
     3061 caaaagtatt tgagacaaat gctgaaaagt atttgactaa actgaaacgg ttggatgctg
     3121 actataccac tagtcttaaa gaagcgaaac aaaaaagttt tgtgacgcaa cacgcagcct
     3181 ttggatattt agctttggat tatggattga aacaagttcc tattgctgga ctaagtcctg
     3241 aagaagagcc atcttctggt cgattagctg aattaaaaga gtatgttaaa aaaaataaga
     3301 tcaattatat ttactttgaa aagaatgcta atgataaaat tgcaaaaact ttggccaacg
     3361 aagctgggat aaaattggaa gtactgaatc ctcttgaaag tcttacaaaa gaacaaatgg
     3421 ataatggtga agattatgtt tcagtaatgg aagacaatct aaaagcgtta gaaaaaacaa
     3481 ctatggttgc tggtgaagag gtagttccag aaaaagaagc gaaggatgaa aagacagttg
     3541 ctaatgggta ctttaaagat gccgatgtga aggatcgcga attgagtgat tatacaggcg
     3601 aatggcaatc tgtttatccg ttgttaaaag atggaatttt ggatgaagtt ttcgattaca
     3661 aagcaaaaat caataaagac atgacagctg ccgagtacaa agattattat actactggct
     3721 ataagacaga tattgatact atcaacatta aagacaacac cattgatttt gtagtcaacg
     3781 gtgaacatca tcaatacaca tacaaatatg ttggatacaa aattttgaat tatgaaaaag
     3841 gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc aggtgctggt cgttttaaat
     3901 atattcaatt tagtgatcat ggaattgcac caagtaaggc agagcatttc catattttct
     3961 ttggtggaga aagtcaagaa aaactctata atgaattgca taattggcct actttctatc
     4021 cagcttcatt gtctaaacat gaaattgctc aagaaatgat ggctcattaa aattcaattt
     4081 ttggaggaaa tacatggtaa gaaaagtatt attgtctatg gattccaaga atattgatgc
     4141 ttatcggcgt tatcgatttg caaagcaaat gagattaaat gggagtacat tagctgatat
     4201 tgctgcaact ttacatgtta gtattgacac agttcataga gtattaaaac gggatcctga
     4261 ttgttgtttt atcaaggtca atactggaag aagtagacgt ttagcaaaac ggcataatag
     4321 cttggccaat taattgataa taccagagta gatcattttt cccttatttg gatttgatct
     4381 gctttttatt atgtcttgaa ttgtcaaatc aagcgcaaca ctttatcaaa gaagacttca
     4441 taaggtgttc tccaatctaa acactttcta ggtctgttat tcagtttttc aatcatctga
     4501 tttatcgttg agttatctac cgtagctatc tctttcgatt ttggcaagta ttctcgaatg
     4561 agtctattcg tattttaatt tgttcattgt tgccaagggc atgaggatct ggaaaataaa
     4621 agggcatatt attcaatgct tctgtaactg cgaaatgatt ggagaattct tttcctctat
     4681 ctggagtgat cgttttgacc gcctcatttg gaatactgct taaaagttca atcattttct
     4741 cgctcacata agcggctttc tttctgggga ccttctttcc tgctaaaagg aagcatgatt
     4801 ttctgtctgt caaagtcact aaacaagtac caccagtttt gccagctaca atatcggctt
     4861 tccaatgacc aaaatcactt ctatcatctg cggattttgg acggccatgg atcgaatgag
     4921 taaggcaaat ctttccacga gactcgacat gattttttgt atgtcttgtt tttcctcgat
     4981 ggtgcaatga acggatcagg cctttattgc cgtgtgagag gggttctgta tcgaaaagat
     5041 cattgcaaat tgcacgttag atcgtgataa aactaattga aaagtccata tgttcatgct
     5101 tcaaacgatt cgagatttcc tctggcgacc actgaaaatc gacaaataaa tgacgaacaa
     5161 ttgaccaaac tcacggatca tttagaatgt gataacgtcc gcattttttt cttcgataag
     5221 cataattttt ttccgctttg gaaggtgaat agtttttttc ttgtttcttc tcagttcatg
     5281 taaaatagta aacgggctat gctgaatgtc gttgccaatt atactaatag aaaatccttc
     5341 ttcgtgaagc agaaagattc tttctcgttc gagtatagta agatgacgat actggctcat
     5401 aattattttc cttctaggag tgttctcgac aaacttatac tagttggata tcgattatga
     5461 ctcattttta tgtgttgcac ttaaagtgta aattcaaggt atagaaagag gttccctatg
     5521 aaaaaaatac ctattacaat cttaactggc tatcttggtt caggaaaaac gatacttcta
     5581 aatcatattc tgtacggtga tcacggaaag gaaattgctg tcattgtaaa tgacataggg
     5641 gaagtaaatg ttgatggggt attagtcgac aagaaaggtt ttaaaagaac tggagaaaaa
     5701 cttgtttcga tgccgaatgg atgcatttgt tgtacattac gcgaagattt gattgttgaa
     5761 ctagatcaat tagtgcaatt ttctgacttg aatcaaattg ttattgaagc ttcaggaatc
     5821 agcaaaccaa ttccaattat tcaagcaatt atattagcta aaaacccttt agacattgat
     5881 ttgacccaac gattagagat tggtgccatt attatagtag tggatgtaaa acgtttttgg
     5941 gataatttcg aaacgggtga aaagattcag gaaagaaaag ctgaaggtaa tgtagttgaa
     6001 gaagaacagg atattcgtgg cttgttaatt gatcaattcg agtgttgtaa catattactt
     6061 ttaaataata gtgacttagt gtcaaccaag acgatttaca aaattaacgg tctaattgct
     6121 aagttacagt ccaatgccaa aataattagg acaattaaag gtgaagtaga cttatcgaaa
     6181 attctaaata caaatctttt taatatagat gagacttcct attcagtggg ctggttcaaa
     6241 gagttagagc ttggctatca aaatcatatt cctgaaacag aagaatatgg tatctcaagt
     6301 tttatctatc gaaatcggtt actccttgac gcacaaaaat ttgcccatct tacggataaa
     6361 aattttcctg gcaatattac aagagcaaaa gggtttcttt ggtttgccca acatagtgat
     6421 ttttcgacga tctttgaaac atcgggtagc tataaaagta ttgaatcatt aaattattgg
     6481 gtagctagtt tacctaaaat tgagtgtcaa atgattatac aaaaaaatcc cgaattgaag
     6541 gaagcctggc atccgttgtt tggtgaacgc attaataaac tagtttttat tggtatgaag
     6601 atggatcaac aatgtattaa ggaacaactt gagggttgtt taactgattc tcttaatgca
     6661 gataatttcc gaaaaatttc accatttcca tggaaaaagt ctaatatgtt tagccagata
     6721 tcgttaatga aatttttaag aaaagctggt gtcaagatag gcgatgtcat aattattggc
     6781 attttgatta ttacctcttt tattccctta tttgtattct ctatagaaaa ggagaatgaa
     6841 acaactgaag atgctatcaa atatgccatc gttcgaattg atggcaagga aatggatcgc
     6901 ttcgatttgg ataagatcaa tcataaactc gtgaattatc atcctgcgaa gggacaatat
     6961 aatattgttg agattaaaga tggaagaatt cgagttaaag aagataatag tcctgatcaa
     7021 atcgcggtta gaactggatg gatctcaaaa cccggtcaaa cgagtatttg tttaccccat
     7081 aagttagtta ttagtattga gaaaaaagaa agcacagatt actacattta ctaatctgga
     7141 aatagatgag gaggttttta ataagaaaaa gatattatta ttaataatag caggggcttt
     7201 attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg gtgacaacag gcacaagtat
     7261 ctttaacggg acctgcaatg gtttagttaa cagtcggtta gattacttat tttggctctt
     7321 cgtcaatgag tgttggtgaa gaaattcaat agaataatcg agatactcac acggctagac
     7381 agctatcttg tctagccgtg ttgttgttct ggctttagtc agttgggtag tattcttagt
     7441 tttcttaaaa tattttcctc gatataaaaa gattcgttct cggtagtttt gaaagtttct
     7501 atagccatag gcaattcgtt ttagtacttt gatatggtta ttcaaacatt ctatagggcc
     7561 gttggaatag ggaacgcgca acgctcgttt gatttctgtt tggtatttct tgaaggtcgt
     7621 gaacactggt tggtagtgct ctggcaactc cttgtagtct tcttttagga gcgcgacgaa
     7681 ttcaggaaca tcttgtcttc tgataacgga aagaaaatcc tggtagactt cgtagcctcg
     7741 ctttaaggaa tcatcataag ataatagccg atcgactatc tctgcttctg tcagatgatc
     7801 tcgaaatgac ggacgccagt agcgatgctg acctttcaat tcccacgcat tcttttgaag
     7861 tagtttccag tatttcttta agtgcttcga ccgacgatcc ccttttttaa aggtcttcat
     7921 tacttgaatt cggtggttgg taaaagcccg accaatgtgt tggctaatat gaaagcgatc
     7981 ggcgacgatt cgggcattcg gaaaacactc tttcaccaat gtgcgatagg gtgtatagaa
     8041 atctgaaacc actagacgca cttgttcacg gacttttata gggtagcgca agaaatagtc
     8101 gcgtaaagga ttgagttgcc gattttccac aatatcaatc agttgatggg tcttgccgtc
     8161 catcatgaca aagctcatgg ctcccgagac atttttcact gatttgaatt catcgaaaca
     8221 caccacttct ggtaacagct ggctcaaaga aa
//
LOCUS       DASJUF010000111         8248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.112, whole genome shotgun sequence.
ACCESSION   DASJUF010000111 DASJUF010000000
VERSION     DASJUF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.112"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            234..1559
                     /locus_tag="U4B62_002369"
     CDS             234..1559
                     /locus_tag="U4B62_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2142500.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="U4B62_002370"
     CDS             1552..1902
                     /locus_tag="U4B62_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142501.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1884..2069
                     /locus_tag="U4B62_002371"
     CDS             1884..2069
                     /locus_tag="U4B62_002371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142502.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /locus_tag="U4B62_002372"
     CDS             complement(2973..3248)
                     /locus_tag="U4B62_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN2142503.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="U4B62_002373"
     CDS             complement(3220..4173)
                     /locus_tag="U4B62_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2142504.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="U4B62_002374"
     CDS             4785..6278
                     /locus_tag="U4B62_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2142505.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="U4B62_002375"
     CDS             6413..6709
                     /locus_tag="U4B62_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN2142506.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="U4B62_002376"
     CDS             6810..7661
                     /locus_tag="U4B62_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142507.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7965
                     /locus_tag="U4B62_002377"
                     /pseudo
     CDS             7682..7965
                     /locus_tag="U4B62_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2863 a         1283 c         1552 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DASJUF010000112         8214 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJUF010000112 DASJUF010000000
VERSION     DASJUF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.113"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..372
                     /locus_tag="U4B62_002378"
     CDS             <1..372
                     /locus_tag="U4B62_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2142508.1"
                     /translation="NKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDR
                     ELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNP
                     NMFVNYQNDPYQFTTQVILLF"
     gene            387..1139
                     /locus_tag="U4B62_002379"
     CDS             387..1139
                     /locus_tag="U4B62_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2142509.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1842..2057)
                     /locus_tag="U4B62_002380"
     CDS             complement(1842..2057)
                     /locus_tag="U4B62_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEN2142510.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2076..2312)
                     /locus_tag="U4B62_002381"
     CDS             complement(2076..2312)
                     /locus_tag="U4B62_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEN2142511.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2570..4723
                     /locus_tag="U4B62_002382"
     CDS             2570..4723
                     /locus_tag="U4B62_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEN2142512.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4692..6107
                     /locus_tag="U4B62_002383"
     CDS             4692..6107
                     /locus_tag="U4B62_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN2142513.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6140..7174
                     /gene="sppA"
                     /locus_tag="U4B62_002384"
     CDS             6140..7174
                     /gene="sppA"
                     /locus_tag="U4B62_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN2142514.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7176..7697
                     /locus_tag="U4B62_002385"
     CDS             7176..7697
                     /locus_tag="U4B62_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2142515.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7694..8080
                     /locus_tag="U4B62_002386"
     CDS             7694..8080
                     /locus_tag="U4B62_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN2142516.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2919 a         1185 c         1477 g         2633 t
ORIGIN      
        1 aataagattt gttgtaaatt cgaatgtcca agtactatta ttgaagttcc tgttccaata
       61 tttgactgta ttagggtgct tggaattatt ctcgataatg caattgaagc atctatagag
      121 agtcaagata gagaactgtc tctagctatt taccgagatg ataaacagat agaaatttcg
      181 atatcgaata gttgtagaga gatactactc tctatagata ctctaatgaa aaaaggaata
      241 acaacaaaaa agggacatca agggttaggt ttatctagta tagaggaaat caataaaaag
      301 aatcccaata tgtttgtgaa ctatcaaaac gatccgtatc aatttactac tcaagttatt
      361 ttgttgtttt gagggaagga ggatttatga gttatccaat aatattatgt gaagatcaaa
      421 ttatacaatt aaatcagtta gaacgaatta ttgataattt cattcttttt cacgataaag
      481 tatttaagat agtattgaaa acacaaagtc cgttagaagt taaaaaatac ctcaaacaat
      541 tccgaccaaa gcaaggaata tatttcttag atattgattt aaatcatgaa gttaacggta
      601 tagaattagc agaagtaatc agaaaatatg atgttcaagc aaaaatcatt tttacaacta
      661 ctcatgatga gatgttaccc gtaacaataa aaagaagagt tgaaacgtta ggatttgtaa
      721 caaaagatca aacactagat gagtatcgaa acgagattgt tgagttattg ttattagcgc
      781 aagaaaggat agatgcaaca aaacaggtta cgaatcaagc atttgttttt tccataggtt
      841 ctcaaacatt tacttttgat ataaatgaca tttattttgt agaagcttca aaattaccac
      901 atagattatc attgtgtact aaagatgggc agtacgaatt ttatggaaga attagtgagc
      961 ttgaaaagaa atatcctatg ttaacaagaa ttagtcgagc gtgtttagta aatatcttta
     1021 acgttaaaga aatagatttt aaaaaacgtt cactatattt tgattcagag ttggccagaa
     1081 actttacttt agggaaagca caaaaaatta aggagaagtt gaaagaaagc actgtttagt
     1141 ggttttttct ttagggagat tgcatttttc taataaaata gattccttca ctagtgcaaa
     1201 tttttctgaa tcctattaaa gttgtaccaa tttttatagc tgttattagt tacgtgcata
     1261 acagcaatat tagttactgg aggaatattt ctatggtctg gaaccaaaaa agggccaaat
     1321 gaatagttct tttcttgttt ttattaagta ccataaaggg gaattaaaat taaatgtatg
     1381 gtggaagatg cgaaagattt ggcaaattct actaattttt agaaagtgtt tttcattttt
     1441 tagttaaaaa atttgtcctg tcaagtgcaa tcctatgatt gtacttgata ggatttgaga
     1501 taattacttt cctgttttta taaatttaac ttgcaggaac aggctttaat gcacatccga
     1561 caagtacatt accaatacct cctgtaacgg ccgaaatagg tcctcctgca gcaccaacta
     1621 cagcgctgca agtccttttg taaaaatatc tctaccatta catttccaaa gaatttttat
     1681 ctgactttaa taaaagataa aaatttaata taattataaa aactagcaca agaagcttta
     1741 gtaaaaagtt ctgtccattc caaacttttg aaaagataaa gaacatacct atgttcgtaa
     1801 tcgacacaaa ccaatcttga ggtttgatat tttttaattg tttacgcacc ggtccacatg
     1861 ctgaatccag cacctaataa agctccacct acaccacaag cagctattgc ataaggtcct
     1921 ccggctgaac aaagttttac tcctgctgtt gccccagaaa aagtagtgcc taaaactccc
     1981 acggcactcc caccttgaat atttttcaac tcttctgaag ataatataaa tgttgttttt
     2041 gtgtttttaa tatgcatact acttattctc cttttttatt tttttcgagc atcccagcca
     2101 gctgcagcac ctccagcgac agctccatat aaccctccaa caacaccgca agctggtccg
     2161 caaattgctg ttcctaaacc tgcaccacct actgcccctg tggtaagtcc acctacaact
     2221 tgccccaatt ttccccaagc attattgcct ccaccaacag tttgtgcatt tccacaatac
     2281 ttagttcctt tacattttgc atgtttaaac acgcttcttt aaataatatt ttttacggtg
     2341 ctactgtaac atacgtattt gattaacagt aaaaatcttc ctgaaactta tcaagtttat
     2401 tcttgaatag taaaatttcc aaaataaatt tgattcagga atgaaaaagt tagtgttcaa
     2461 gaactgtttt caaagttttt gtggaattat gagacaatat tttcactgta ttaaagctat
     2521 attgagaatt tttctataaa aattttttag aaggagttgt gttagataag tgataaaatc
     2581 atttcctttt gtgcagcaac aagatgaaaa agattgtggt gttgcttgcc tttcgatgat
     2641 tttaaaatat tttaaaactg atatcccgat tcataaatta cgggacctat ctgggactga
     2701 tttagagggt acttcagcat ttggattgaa aaagacattt gaaaaattgt cttttgattg
     2761 tatggctatt caagcagata gtgagatctg gaaagaaaga gatcttatct taccattaat
     2821 tgcacatata ttggttgata acagctatat gcactacgtt gtaatttata aaacgaaggg
     2881 aaacgtacta tggattgcag atcccgctaa tggaaaaaat agaaaaacga ttgaagcgtt
     2941 tcaaaaagaa tggacaggta ttcttttatt acctatgcct agagaaactt atattcctat
     3001 taaagaaaaa gtagctggat taagttcttt tttgcctata atttggaaac aaaaaggatt
     3061 agtattccac atcgttttag catcgatttt cattacgttt tttggcatag gaagctcata
     3121 ttattttcaa ggtattcttg attttttcat ccctaatcaa gcacgttcaa ctttaaatat
     3181 aatttccatt ggcttgatcg ctgtctattt atttcgggta ttgtttgaat atagtcgtag
     3241 ttatcttctt attattttag gacagcgaat gagtatggca attatgcttc agtatttcaa
     3301 acatgtgtta actttaccaa tgaatttttt tgctacgaga aaatcagggg aaattatttc
     3361 tcgttttctt gatgccagta agattattga tgctttagca agtgctacgt tatcaatatt
     3421 tttagatatt ggtatggtgt tgcttgttgg ggttacgtta gctattcaga atagtacgtt
     3481 gtttttaatc actctatgct ctttgccttt ttatgtagtt gcaatattcg ctttcgtgaa
     3541 aagctatgaa aaatcgaaca gggaagaaat ggcagccggt gcagctgtaa attctagtat
     3601 catagaaagt cttaaaggaa tagaaacaat taaagcatat agcggtgaag agaaagtata
     3661 taaccgtata gaccatgaat ttgttaaatt aatgaaaaaa tcatttcgta cggttacgct
     3721 tgataatatt caacaaggaa ttaaacattt gattcaatta attagcagtg cattaatttt
     3781 atggctaggt tcttattttg ttatggataa tagaattagt ctaggtcaac ttatcacata
     3841 caatgcttta ctagtatttt tgacagatcc tttacaaaac attattaatt tacaagtgaa
     3901 aatgcaaacg gctcaagttg caaataatcg tttaaatgaa attttcgcga ttgattcaga
     3961 aaaatcgaac cagtcttttg aatatatgct ttctgaacaa ttatttcaac aagatatcac
     4021 tgtaaaaaac gtttcattct cgtatagcat gaaggcccca acgttgaacg aaatttcatg
     4081 tacaattcct tcgcacagta aaacggctat agttggagtt agtggttctg gaaaatcaac
     4141 tcttgcaaag ttatttgttc gtttttatga agcatcagaa ggatcgatcc attatggaaa
     4201 cattaatcac ttagacattc cttttcagat attacgcgat cgagtgacat atttgcctca
     4261 agagtcattt ttctttagtg gaacaatcat tgagaatctt atattcggtt tagatagccc
     4321 accaagtttc gaacaaataa tagaagtttg tgagatagtc caactaaatg aatttattaa
     4381 tcaacagcca ttacgttacg agacattgtt ggaagaaggt ggaaataatc tttcaggagg
     4441 gcaacgacaa cgattagcct tagcacgggc tttattaaaa aatccggaca tattaatttt
     4501 agatgaagca actagtggat tagatacctt gttagaacat gcaattatga attatctcct
     4561 caatttaaaa gaaaaaacga ttatttttat tgcgcatcat ttaccaatag caaaagcatg
     4621 tgatcaaatt ttagttctcc atgaagggca tttggtagaa aaaggaacgc acgatgaact
     4681 tcgttttgcc gatggtatct atcaaaaatt atgggaaatc taggagaggg aaacaattta
     4741 tgcagaataa taattggttc gaacattcta gtatctatac taaacaacat catagcttct
     4801 accgttgggt gttataccct gtaattcttt ttttatgtat tattggattg tttttaacct
     4861 ttgctaaaaa agaggttgtt attcggacaa cggctcaatt gaaccccgaa aaaatagaga
     4921 agttacaagt gccattagaa gctaaaataa tggaaaactt cctaaaagaa aatcaatttg
     4981 ttcataagga cgatgtgttg gttagattgg attgttcttt aatagagaat gaaaaggctc
     5041 agatagaaca agaaaaccaa cgtattactc aacaaataaa gatggctcag ctatttattg
     5101 aaagtataag taaaggaaaa aatttgtttt caacggatga cagttttggc tacagtaatc
     5161 aattaaagag catgttgtca gaaaaagaat cactccgcta cgctttgaag caaagtgaat
     5221 taaatgatca aaagcaatta gaagtatacg aaaagacaaa aagacaacta gaaaaacaaa
     5281 ttgagagttc agatagtaaa ttacaagaat ggcaacaagt acaggtagct tggagtaata
     5341 atcaatcatt aaaagatttt tcaaaagaaa tgatggcaaa ctatgagaat tggcaagaac
     5401 aactaaataa tgtttctgat gatcaaaaaa atcaagtgaa actgacaatt tcagcaagca
     5461 taaatgaaca aattgagcaa ctaaaaaaag aagtagaaca gtatcagtca gaaaaagcta
     5521 aattagttaa accaactact tctgagaatg acagaattag tcaaacggaa aaaggaaagc
     5581 aagagctaga acaagcaata acaactgtaa aacaaacagt tacagagcta aatgaaaaac
     5641 aagagaagaa ccagtcagtt atcaaaagtt tggacgatca actttcaaaa ggaatactaa
     5701 aagctcctgt atctggaacg gttcatttaa atgaagaaac aaagggacaa gcagaactag
     5761 ccaaaggaac agtgcttgca gaaatttatc cgatccacga aaaatcaaaa atgaaattta
     5821 cagcactttt acctgcgaat gaaagtacct atattaaaga agggatgaag gttcatttta
     5881 aattggatca aaaaggtggg gctccgatta cgatcgacgg aactttagat gaaatttcag
     5941 aaaattctac tgccactgag aaaggtgttt tttatgcagt gaaaggaacg ttaaaaacaa
     6001 ctcaatcgtt tccgtttcgt tatggattaa ctggagagtt gtctttaatt gtaggagaaa
     6061 aaacatattg gcaacaaatt gtcgatgtgt ttctaaataa aaaataatta tttttactaa
     6121 gaatatagga ggagtttata tgaataaaag aagatggatt gctgtgggag tagcgtgcgg
     6181 attattgtta ctatccatta tcgtcgcgct aattccagga aaagataaag aggaagcatc
     6241 aaatacaacc ctaacaggaa taaataagat tttttacgga tctaacgaaa tcacagaaga
     6301 aacgttagaa gaaggcgcat caaataaaaa gattgtcaaa ctatccgtca atggagttat
     6361 tgcagataca ggtgaaagca atctttttag tagggaacaa tataaccatc agaatttttt
     6421 aacacagcta aagaaaattc aagaagacaa agcggttaag ggagtgttat tagaagtaaa
     6481 ttcacctggg ggaggaatat atgaaagtgc tgagattgct aaagaaatgg caaaaattaa
     6541 aaagctagat attcctatct atactgcatt taaaaataca gcggcaagtg gagggtacta
     6601 catttctgct ggttcagata aaatatttgc tacggaagaa acaacaactg gatcaattgg
     6661 tgtcattatt tctggtttaa attattctgg cttattagaa aaacttggcg taactgatgc
     6721 gacgtataaa agtggtgcat taaaagatat gatgtcacct caacacaaac cttcagagga
     6781 agagaataaa gttatacagg aatttgtgat gagtgcttat gatcgttttg tcaatgttgt
     6841 agcaaaagga agaaatatgg atactaatgc agtgaaagaa cttgcggatg gaagaattta
     6901 tgatggtaac caagcagtag aaaatgggtt ggtggatcaa attggttact ctgaagatgc
     6961 attagattcc ctaaaaaaag agaagaaatt aactgatgcg acggttatag aatataaaaa
     7021 tgacacaact ggctttgctt cttcttggtt aggtaataag attgctgaat ggcaagggct
     7081 aaaggcaact acatcagatc gtgtaatttc agtttttgaa aaacttggat tttctgatac
     7141 tccaaaacca atgtattact atggagggct ataaaatgaa taagaagagt ggcattgaaa
     7201 ctttgcctaa ttatgtattt gcgggatttt ggttacgatt ttttgctttt cttattgatt
     7261 taatttgtat tagcatcatc acccatgcta ctataggtct cttttacaaa atcatgggac
     7321 tggaatctga caaatccatt attacttttt atggtatagc gtcattagct atttatttaa
     7381 cctattttgt acttttgact aagctgaatt caggacaaac aattgggaaa atgatttttg
     7441 ggataagagt agtgagcttt gaggaaaaag agctaagttg gcaaactatc tttattcgtg
     7501 agtgtatatg taggtttata ttaaaaaatt ttgtatttca aattgggtat ttggtggctg
     7561 ctttcacgcc caagaaacaa catattgggg attatttttc taacacgtca gtggttgtta
     7621 ttcgttcatt agaagctatt aactatggtc aaaaaatttc taattctaaa acacttaatc
     7681 tggagagaat taaatgaata agagaaaaac agagtttatt ttgattttaa ttagtggaat
     7741 aacagggatt ttatgtagtc ttttagcatt gatgggtggt atgtttctat cttctattga
     7801 ttcaactact ttaaatacta cagaactaac cagtaaggat atagatatgt tagtcaatct
     7861 aagcgggaat gttgttttat ttggaggaat taccctagtt ttttcgataa tgattttcat
     7921 aataggtttt ttagtaagaa aaaatcaaaa aacagtggtc tatggtatta gtttactcat
     7981 tttatctatt attccatttt ttcttttaac attattgtgg gtaattcctg gagttttagg
     8041 tattattgcg ggattgatgc ttctaataag aaaatcttaa ttaggcaatg tgtagacaac
     8101 gtgaaacgat taatcaatta aaagctaaat acgttttgag aaatgataac ttgtagtcga
     8161 tggaagacat gtagataaaa aaactacgtg tcttttattt attctaattt tttc
//
LOCUS       DASJUF010000113         8080 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.114, whole genome shotgun sequence.
ACCESSION   DASJUF010000113 DASJUF010000000
VERSION     DASJUF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.114"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            316..1854
                     /locus_tag="U4B62_002387"
     CDS             316..1854
                     /locus_tag="U4B62_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN2142517.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1851..2816
                     /locus_tag="U4B62_002388"
     CDS             1851..2816
                     /locus_tag="U4B62_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142518.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2803..4125
                     /locus_tag="U4B62_002389"
     CDS             2803..4125
                     /locus_tag="U4B62_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2142519.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4188..4292
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4366..5109
                     /locus_tag="U4B62_002390"
     CDS             4366..5109
                     /locus_tag="U4B62_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2142520.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5124..6116
                     /locus_tag="U4B62_002391"
     CDS             5124..6116
                     /locus_tag="U4B62_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2142521.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6128..6865
                     /locus_tag="U4B62_002392"
     CDS             6128..6865
                     /locus_tag="U4B62_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142522.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6855..7577
                     /locus_tag="U4B62_002393"
     CDS             6855..7577
                     /locus_tag="U4B62_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2142523.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7661..>8080)
                     /locus_tag="U4B62_002394"
     CDS             complement(7661..>8080)
                     /locus_tag="U4B62_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN2142524.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2581 a         1402 c         1759 g         2338 t
ORIGIN      
        1 ccaataaagg tgttctcccc cacgagattt ctactttatt ggctgtttct ttttattaaa
       61 ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa gtgacaattt cgtatagatt
      121 tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt gatccgactt tgttgcttgg
      181 attctttttt tgtcagcata tttagaggta tttttttctt ttttttgttc gtgctgtaag
      241 gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat gtttatactt aaagaataac
      301 agaaaggaag gtcgcatgaa taattttata caaacattaa gtgaaagaaa aggcgagttg
      361 ctggaagctg catttcaaca tctgtccatt tctctcattt ctttgctgat tgcagcactg
      421 attgctattc ctttagcgat ttgggcagcc aatcataaaa aaatggcaga agtcttattg
      481 cagatcacgg gtgtgctcca aacgattcct tcacttgcct tattaggttt acttatccca
      541 tttgtaggga tcggaactgt tccagcattg attgcacttg ttatttacgc tttgcttccg
      601 attttccaaa acacgtatat tggtctagct gaaattgacc cgtctattga agaagcagcg
      661 gtagcatttg gtatgtccag aatgagacgc ttgctgaaag tagagcttcc catcgcatta
      721 cctgtcatta tctcaggtat ccgaactgcc ttagttttaa tcatcggtac ggctactttg
      781 gcagcattga tcggagcagg tggattaggg acatttattt tgttaggtat tgaccgaaat
      841 acacctgttt tgacattgat tggtgcgatc agttctgcct tgcttgcaat tatattcagc
      901 ggactgattc gctggttgca gcataagaaa cctcgatatt ccatcattac tttagcagca
      961 gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg ccttgtttaa aaatgaaacc
     1021 atcacgattg ctggaaaatt aggagcggaa cctgaaatcc tgatccaaat gtataaagaa
     1081 ttgattgaag acaacacaaa aacagaagtt gaattaaaac cgaattttgg caagacgagt
     1141 tttttgttca atgcactgga tcaaggtcaa atcgatatct atcctgagtt cacaggtact
     1201 gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa gtgctgattt gacaaaagaa
     1261 aaaacatatg acgaagcaga agaattattg aataagcaat tcgatatgac attattaaaa
     1321 ccaatggctt accaaaatac gtatgccttg gcagtcaaga gatcttttgc gaacgaacat
     1381 caacttaaaa agatttccga tttggcagct atctctgatc agatcaaagc gggattcaca
     1441 ctggaattta ttgatcgttc agatggatat aaaggaatcc aagaagcata tggcttgaat
     1501 ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc aagcaatcaa taatggagat
     1561 gtcaatattg tggatgccta ttccacagat agccagctca aggagtacga tctagtgact
     1621 ctagaagatg atcgagggtt attcccagct tatcaaggcg caccgctgat gaaaaaagat
     1681 tttgctaaag aacatcctga aatcgttaaa gcactgaata aattaagcgg aaaaataaca
     1741 gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg aaaagaaaaa gccgacagaa
     1801 gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg aggaagatca gtgactaaat
     1861 taatcgagtt caaacatgtg caaaaaaagt atgatggaaa gtatgtcatc gatgacctaa
     1921 atctggcaat caaaaaagga gaaatctttg ttttggttgg tccttccggt agtggaaaaa
     1981 caactacttt gaaaatgatc aatggattat ctaaaccatc agcaggagac atctacttca
     2041 aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg gtggaatatg ggatatgtgc
     2101 tgcagcaaat cgctttgttc ccgaccatga cagtcaagca aaatattgaa gtcatccctg
     2161 aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt agatgaactt cttcaaaaag
     2221 ttggcttatc tcctgatatt tatcgagatc gtatgcctag agagttgtct ggaggagaac
     2281 agcaacggat cgggatcatc cgagctattg ctgcaagccc agatgtgatt ttgatggacg
     2341 agccatttag cgcattagat ccgatttcaa gaaactcatt acaagaattg gttttgtcct
     2401 tacacgaaga attgggcacg accatcgttt tcgttacgca caatatggaa gaagcaatca
     2461 agctaggtga tcgtattgca tttatgaagg atggagaaat tatccaatgt gacacaccag
     2521 agcagttatt gatgaatcct aaaaacgact acgttcggca tttctttgat gaaccgaaac
     2581 agacgaaaga gtggcgagta gaagatttag tcgtcaatgg atatttctta aatgaaatac
     2641 cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat gaaagaagta tattcgcttc
     2701 tttcggtgta tccaagcatt gtgatagtag aaaagcaaag aagtattggg tcgctgacaa
     2761 gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg aaatgaaaac atatgatgca
     2821 atcatcatcg gttcaggtgt gagcggctta agtgctgctt atggattgaa agaagcaggt
     2881 aaaacagtac tagtggtcga agaagatcta tggggcggga cttgtcctaa ccgtggatgt
     2941 gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta atcgggtaaa acaattgagt
     3001 ggaaaaggtt ttaacgagat cccaactgcc aactgggaag aacttcaaaa attcaaacga
     3061 acatttacgg atccagtccc agaaagccga aaaaaacaat tagcagaagc tgaaatcgat
     3121 catctttcag gaaccgctcg ttttttggat gattcatcta ttgaagtcaa cgaagaagtc
     3181 ttccatgcgg attacttagt tttggcaacc gggcaaagac ctacgatctt accagtggag
     3241 ggaaaggaat acttgaaaac tagtgcagac tttttatcat tgcctgtact gcctaaagaa
     3301 atcattttta tcggtggtgg ttatattgct tttgagctag ctacgattgc caatgctgca
     3361 gggagcaaag taaccattgt ccatcataat caacggccat taaaagaatt tgaggcgtct
     3421 ttggtagagg aagccgtcca tcaaatggaa gcatctggca ttcagtttgc ttttggtgtt
     3481 gaaacccaaa agatcatttc agaaggtact cgctatcgtt tagtcggaaa agagacggaa
     3541 ttagtagcag atatgatttt ttgtgcgact gggagacagc cgaatacaga atctttagca
     3601 cttgagcaag cgaatatcgt ctttgataaa cacggaattg cagtcaatga ttatctgcaa
     3661 acgagcaatc caaaaatttt tgcgtgtgga gatattgttt caaggaaaac cccaaaattg
     3721 acaccagtcg ccacttttga aggaaattat gtagctaagc ggataacgga tgcaacaagc
     3781 gaacctatca aatatccgat tatacctacg atcgtctacg ccagtcctaa acttgctgaa
     3841 gtaggagtta caaaaagtca cgcctcttcg tcagatcaag tggtagagat ggatcttacc
     3901 agttggttca cctatcatcg tgtcaatgaa ccagttgcta aggctgaatt gacctttgac
     3961 cagcaaaatt atttgattgg tgctgcagtg attagcgagc aagcagacga attgatcgat
     4021 gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag agttggatag ctatatcatg
     4081 gggtatccga cattggcaag tgatctctcc tatttattga aataatcagc atgataacta
     4141 aagcgcttgt tgctttttcc ttcttgtgct acactttaaa aaatgaatac ctgtttctgg
     4201 ggatgtcgta gacgacatga gagtaagttg tgaacttaga cccattgaac ctgtcagtta
     4261 atactggtga agggagaaac atagcagacg cttgaagcgg gttactatgt cgtgcccatt
     4321 tcaagcgttt tttgttattc atattagaag aaaggaggta acaaaatgaa gaaaaatctt
     4381 tatccagcag tcagtatggc attcatttta attatttggc agattgttgt catagtttgg
     4441 aaaatacctg cctttatttt acccggacct gctgctgtat tttctgcatt agcggcagat
     4501 gcacaagtat tactttccca ttctgtcacg acattaaatg aagcagtgct tggtttggta
     4561 atagctagcg ttctttcttt tattactgcc ttggcaatgg attattggaa gtttttagaa
     4621 atgtcttttt atccgttact agtgatttca caaatgttgc caatcatggt gttagggcca
     4681 ttacttaccc tatggtttgg tttcggtatg cttccaaaag tgatccttgt tgtcttgata
     4741 agctattttc cgatcgtagt ttctttttcg gatacattga ggaaagtatc aaaagagcaa
     4801 atcatctttt tgaaaacgat gggggcagat acagggaaga tttatcgaat atataaaata
     4861 ccagcgggtt tatccggctt cttttccggt ttaaaagtgg ctgctactta ttgtgtcagc
     4921 ggagcagtcg ttggagaatg gctaagcgct caagcaggct tagggtatta catgatacgc
     4981 gtaaaaaaca gctaccagat cgataaagtt tttgctgcga tcatttgtgt cattctattg
     5041 agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag ggtattatcg tatgctttat
     5101 attttttaag agaaggggat caaatgaaaa aattatggct tttattaccg ctactgttgc
     5161 ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa acaagaaata aaggatttga
     5221 aaaaagtgac acttgttttg gattatgtac cgaatactaa tcatactggc atctacctag
     5281 caaaagaaaa aggctattat aaagaggctg gtttaaatgt ccaaatcatc gaaccaggag
     5341 ataattcgac aagtattggt ctagtaggtg cggacaaagc acaatttggt gtaagttatc
     5401 aagaagatgt cacttatgct cacgcagatg gacagaatat accagtcaaa gcaattgcta
     5461 cagtgatcaa acataatact tctggattcg ccaccctatc agacagtaac atccattcac
     5521 caaaagattt tgaaggaaaa acatatgcag gttggcaaag tccaagtgaa gaagctgtct
     5581 taaaagctgt gatggaaaaa gacggcgggg acttttcaaa actgacaatg gtaggaagca
     5641 atggagaagg gccagaaagc ttaggaaaat cctcagatat ccaatggtac tttgaaggtt
     5701 gggatatgat caaagcaaaa gaagcgggta ttgaactaaa ctatatccca ttgaaagaat
     5761 tagatgaacg gttagattac tatactccgg taattattac gaatgatcaa ctgatcaaaa
     5821 gtgatccaga attggtacag tcttttatgg atgccacgaa aaaaggctat caggaagcta
     5881 ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata tgcaaaagaa aatgatcgga
     5941 cgtttttaga agaatcacaa gcattcttgt caaaaaatta tacggatgat cctgagaatt
     6001 ggggattgat ggaagaaaaa gtttggcgca attatacagc ttttatgcag gaaaacggat
     6061 tgatcaagca agatgtacct tcgcaagaac tattcactaa tcagtttata aaataggagt
     6121 gatagatatg tcaatccaaa tcagccactt gaatgtagtc attgaaaacc tgaaaatatt
     6181 agaagacatc catttcacta tccctaaaga tacctttaca tcgattgtcg cgccaagcgg
     6241 agccggtaag tcaacattgc tgaaaacttt aacaggagtc cagcctatga cttctggaac
     6301 gatcaaagta gacgatcaaa aagtaactgg tctccataca gcatttagtt atatgccgca
     6361 agaagatatg ttacttcctt ggctgagcgt ctatcaaaac gttacgttat atcaaaaaat
     6421 caatcatcta acaatcaatg aagaacaagt aagggattat ctggatatct ttgggttaaa
     6481 cggctatgaa cattttttgc cggaacaatt atctggcggg atgaagcagc gaacggcctt
     6541 gttacgaaca atcatgaatc cttcttctta cttgcttctt gatgaaccat ttggagcgtt
     6601 agatgctatg accagagggc agatgcaaga ctggatgttg aagctgccta aaaaagcaaa
     6661 gcggactact ttacttgtga cgcatgatat cgaagaagca atttatttat cagacaggat
     6721 tcttgtgctt tccgcccgtc cggcacatgt gattgctgag attcaagtgc ctgaaaaacg
     6781 acggtcacgt gaatggctac tgcagcaatc agagttgaag caaacgattt atcagttatt
     6841 ggcaggtgaa ttagatgtta agtgatgctc attgtcattt agatgggagt cagagtttag
     6901 cactccttca gcaggaacac acgattttga cgatcatcaa ttgtgattcg ccagaagaat
     6961 ggaaggaaaa cagacagctt gcagcaagta agactcaagc cttaagttac ggtatccatc
     7021 cttggaaagc agatagctat acgtttgaac aagttgagcc ttttttgaaa aaagcaagga
     7081 tcatcggtga aatagggtta gataatattt ggacgaatgt accgatgact acacaaaaaa
     7141 aagtatttga gcgacagctt gcttttgcag ccataaatga gaaacctgtt gtactgcaca
     7201 caaaaggatg cgaaaaagag atcttaaatt atatccaagc ttaccctaat cactatttga
     7261 tccattggta ttcttctttg gactatcaac aagactatat tgatttaggc tgttatttca
     7321 gcattggtct tgatttgaaa aagaatccag cagtctggca actagctaaa gcagtcccaa
     7381 tcgaccactt acttattgaa acggatggac tacatgcagc agagtgggct ttagggggga
     7441 ttttttcaga aaaagattat gtctcactgc ttgaagaaca gttaaagaca atcgctgaga
     7501 tcaaagagct atcagttgaa gaagttaaaa aaaatactta tcaaaacttg gaacgtttca
     7561 ttagattagg taaataaaaa agagatgtga cacaactagt gtcgcacctc tttttggctt
     7621 tctttgtata aaagtaaact gtttgattaa taaatgaatc ttagtaccaa ccgtttgctt
     7681 cccagaatgc tttcgccgct tcccaagaac cataacgtga agtaacatat tgttctgcta
     7741 ctctttcttg gtttgcagca gagtagtcac catttaagta agaagaatct aattggtaac
     7801 gtccgatgta acgtccgttc gttgctgtgt aagaaccgct tgattctttt tgtgcgatcc
     7861 attcttttgc agagcttgtg cttgctggag tagcagcagt ttctgttaca gcaggagcag
     7921 gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg aaccgtttct tgtacaacag
     7981 gttgttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca gctgcttgtg
     8041 cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DASJUF010000114         8069 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.115, whole genome shotgun sequence.
ACCESSION   DASJUF010000114 DASJUF010000000
VERSION     DASJUF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.115"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..306)
                     /locus_tag="U4B62_002395"
     CDS             complement(133..306)
                     /locus_tag="U4B62_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2142525.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="U4B62_002396"
                     /pseudo
     CDS             754..3116
                     /locus_tag="U4B62_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="U4B62_002397"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="U4B62_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN2142526.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="U4B62_002398"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="U4B62_002398"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN2142527.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="U4B62_002399"
     CDS             5704..6489
                     /locus_tag="U4B62_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2142528.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="U4B62_002400"
     CDS             6502..7353
                     /locus_tag="U4B62_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN2142529.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="U4B62_002401"
     CDS             complement(7477..7929)
                     /locus_tag="U4B62_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142530.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2714 a         1373 c         1710 g         2272 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattc
//
LOCUS       DASJUF010000115         7975 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJUF010000115 DASJUF010000000
VERSION     DASJUF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.117"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..639)
                     /locus_tag="U4B62_002402"
     CDS             complement(<1..639)
                     /locus_tag="U4B62_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN2142531.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(641..1405)
                     /locus_tag="U4B62_002403"
     CDS             complement(641..1405)
                     /locus_tag="U4B62_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN2142532.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1413..2090)
                     /locus_tag="U4B62_002404"
     CDS             complement(1413..2090)
                     /locus_tag="U4B62_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN2142533.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2127..3512)
                     /locus_tag="U4B62_002405"
     CDS             complement(2127..3512)
                     /locus_tag="U4B62_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2142534.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3722..3806)
                     /gene="ffs"
                     /locus_tag="U4B62_002406"
     ncRNA           complement(3722..3806)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U4B62_002406"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3829..4344)
                     /gene="tadA"
                     /locus_tag="U4B62_002407"
     CDS             complement(3829..4344)
                     /gene="tadA"
                     /locus_tag="U4B62_002407"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN2142535.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4451..5308
                     /locus_tag="U4B62_002408"
     CDS             4451..5308
                     /locus_tag="U4B62_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142536.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5389..5724)
                     /locus_tag="U4B62_002409"
     CDS             complement(5389..5724)
                     /locus_tag="U4B62_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142537.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5791..7161)
                     /locus_tag="U4B62_002410"
     CDS             complement(5791..7161)
                     /locus_tag="U4B62_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN2142538.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7239..7775)
                     /locus_tag="U4B62_002411"
     CDS             complement(7239..7775)
                     /locus_tag="U4B62_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2142539.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2305 a         1686 c         1279 g         2705 t
ORIGIN      
        1 tgtttgtttc aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa
       61 tacaaaaaat aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg
      121 atcagtaacg ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat
      181 aaaaaagcga tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc
      241 gatttgaaac ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa
      301 caaaatactc gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt
      361 cacttcataa aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat
      421 ggttttgaag ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc
      481 ttgatctaaa gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg
      541 tcgaaagttg atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa
      601 tccagcatat tccatccact gccatctcga atttttcata ttagaagctc cattcatcca
      661 gtgattgttt cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa
      721 aacctgataa aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt
      781 catagttatc tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt
      841 tatccataat gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct
      901 ctacaaaaga gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct
      961 tatctgggtt cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta
     1021 ctttttcata ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt
     1081 cccatgtaaa tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga
     1141 cttttttccc ttttccatct gctttcataa aatcgattgt tgccaaagta gctgtgtaga
     1201 ctgtctctgg agctacatga atgtcaaact catctgctag acgattagct actgtttctg
     1261 gtgacttagt cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt
     1321 ccacaaatcg tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat
     1381 caattaaata tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat
     1441 gctgtttttg ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt
     1501 tttgctgact ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt
     1561 ccttttttct catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt
     1621 gtcttttctt ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa
     1681 tattcgtaaa gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta
     1741 tccgcttgga aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa
     1801 tcatatcggg ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca
     1861 cgataatcgg acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac
     1921 agtgtcacta gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa
     1981 gcagctgtca gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca
     2041 atttctgttt tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca
     2101 cctatctttt ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct
     2161 cctaccacac ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc
     2221 gttggaaaaa atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc
     2281 agatcgattg gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca
     2341 ttatagacca tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta
     2401 cgtaaaaagt tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc
     2461 aatgtgacat ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg
     2521 acccgccctt ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc
     2581 tttcctcgtt ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata
     2641 tctagactca gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca
     2701 cgttctagat aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta
     2761 gggatggctt tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact
     2821 tcaccgacat actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga
     2881 ccttctctga ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct
     2941 tttgcaattt ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact
     3001 tgtgaacgta aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag
     3061 ccattcggat tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc
     3121 tggtgtttcg ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt
     3181 ttatcgaata agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac
     3241 tcttctttgg aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact
     3301 tcattcatca gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga
     3361 tccacaaaat cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct
     3421 cgtttacgtt gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga
     3481 tcattcgtat gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat
     3541 cataccatag gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc
     3601 ttgaagcaaa ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac
     3661 attcattttt acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc
     3721 attataatca gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc
     3781 ttcacaagcc caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt
     3841 cgttgaccag tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt
     3901 caaaatcatc ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa
     3961 ccgctcgtcc tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc
     4021 gccgaaataa acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg
     4081 ctctaaggta acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc
     4141 ttccttgatt gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt
     4201 atatcctctt ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg
     4261 aagcatttct gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac
     4321 tgcatcaaga tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact
     4381 ttagcatatg ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga
     4441 ggtgaacaag atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa
     4501 ctctccagca tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat
     4561 caaacaagat tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat
     4621 caacgatcaa aaaaaacacc catggtatcg ctatcttttt aaagatgctt cttgtgttca
     4681 agaaaaaagc tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa
     4741 agaatggcaa gatactattt tggaattatt tcgctattct gctgatttct ttttttatac
     4801 agacacagat gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg ctactgaaat
     4861 caacggtatt tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg
     4921 ttctttccat tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt
     4981 tttaacagag caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc
     5041 tttacattac tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga
     5101 actgatcaaa caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa
     5161 tgtcagctct gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga
     5221 aaaatttcaa gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta
     5281 tcttttattg cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt
     5341 gtcacaaccc cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt
     5401 ttcaacaaaa tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc
     5461 ataaacagag attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc
     5521 caagtcacta tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt
     5581 gtcatagacg aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac
     5641 ttttcccgat acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat
     5701 tgttactttt gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc
     5761 atagacaaaa aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa
     5821 gttctttact gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag
     5881 caaaacccat ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca
     5941 ttccttttga gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa
     6001 tgcatagcta ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac
     6061 gacttttaaa ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa
     6121 cactaaaagt cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt
     6181 ttcctgctct tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat
     6241 ctgattttgt ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg
     6301 aggccatata cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga
     6361 actaacggat tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca
     6421 gctaatgatc tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc
     6481 tttatttttt agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta
     6541 tagcggagat tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc
     6601 tcctgaacaa ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag
     6661 ttctgcgggt gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc
     6721 tgataaaatc ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt
     6781 catttgagca gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt
     6841 ttcaccgggt tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata
     6901 ttcataccca gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt
     6961 gctgtaggga gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc
     7021 cggttttagt ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa
     7081 aaaatcggat gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa
     7141 cgttgtcgct tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga
     7201 aaaataaagg tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag
     7261 atattttcca agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca
     7321 agaaaatctt gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat
     7381 tcaagcgact tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc
     7441 ttaaatagcg aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg
     7501 atagaaaaaa aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca
     7561 gctaagtagt tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg
     7621 attgatttga aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct
     7681 cttgcagtaa ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa
     7741 tattctttac tataatttgt tactctcccc atcattctta cctccgtctc ttttcatcta
     7801 cccataaata aataacataa ataattctaa aaatattatt gataattatc aaaacatatt
     7861 tagttatatc cattaaaaat caatttttcc gccttaaaaa caagaatatc ttgacaaaat
     7921 accctaggaa ataaacaata gaataaacaa ggtaacaaaa aaattaaata acgtt
//
LOCUS       DASJUF010000116         7830 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.118, whole genome shotgun sequence.
ACCESSION   DASJUF010000116 DASJUF010000000
VERSION     DASJUF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.118"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            270..1133
                     /locus_tag="U4B62_002412"
     CDS             270..1133
                     /locus_tag="U4B62_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2142540.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1262..2884)
                     /locus_tag="U4B62_002413"
     CDS             complement(1262..2884)
                     /locus_tag="U4B62_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2142541.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3133..3654
                     /locus_tag="U4B62_002414"
     CDS             3133..3654
                     /locus_tag="U4B62_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2142542.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3840..5570)
                     /gene="spxB"
                     /locus_tag="U4B62_002415"
     CDS             complement(3840..5570)
                     /gene="spxB"
                     /locus_tag="U4B62_002415"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN2142543.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5735..6553
                     /locus_tag="U4B62_002416"
     CDS             5735..6553
                     /locus_tag="U4B62_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN2142544.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6656..6853)
                     /locus_tag="U4B62_002417"
     CDS             complement(6656..6853)
                     /locus_tag="U4B62_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142545.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7022..7600
                     /locus_tag="U4B62_002418"
     CDS             7022..7600
                     /locus_tag="U4B62_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2142546.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7695..>7830
                     /locus_tag="U4B62_002419"
     CDS             7695..>7830
                     /locus_tag="U4B62_002419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142547.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2340 a         1415 c         1552 g         2523 t
ORIGIN      
        1 ctctaatact caggtcaaaa cgctgatttc acaacctctg attcacggtg ttccagaagt
       61 agcttctacg gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc
      121 cttcttattt cttgaagctg accacttctg tcacaacctc ttattttgat tagaacttgc
      181 gaaaataaac cggttaaagt gtgtctagcg caagagattt gctataatag acgataaggg
      241 taaaatgtaa cgagaagggg ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg
      301 atctgccata ccatgtatta gcaaaagaaa aagttgattt tttatcaatg aatctccaat
      361 tgaatgatca agagttagta gatgacctcg gaaagacatt caattatgac cgctacatga
      421 atcagctgaa aaatggtgcg atgcctacga catcacaaat caatgttggc cgatatttag
      481 aatttttccg ttcttatgcg gaaaaacaga ttcctgtttt atatgtggct ttttccagtg
      541 gattaagcgg ctcttacagt agtgcgcttc aagcggttga attgctgaag gaagaatatg
      601 aagaccctga gatctatgtt tttgatacaa aagctgcaag tattggggaa gggcttttag
      661 tattggaagc tgtaagattg aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc
      721 tggaaagtaa ttatttgaaa gtccattcat gggtaacagt agacgatctg aaatatctag
      781 aacgaggcgg taggatttcc aaagcaagtg ctactcttgg cagtctattg agtgtaaagc
      841 ctattattac ggtggataaa gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa
      901 acaagtcgat ccagacaatc gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc
      961 aaatcgttta tgtggcttac gctggggatc tagagagcgc ggagaaggca aaacggcttt
     1021 tggaagagca gataaagatg aaagatgtga agctttatcc cttaggacca acgatcgcca
     1081 gtcacacagg ctatggctgt atcgcgattt tttcgatggg aatcagtcga taaaagcagt
     1141 aactcattct gtaaaaaagc ccagaaattg attgtaacaa aaacagcgac tcttaaaaga
     1201 tagatgagca tcctatctct tagaggtcgc tgttttttta ttttttaagc taaataggcc
     1261 attatgccca taactcgttc aattgttttt gaatatctgc attttccaaa aattcatcat
     1321 atgtcgtatc agctcgatcg atcacacctt tatccgaaac ggcaatgatg cggtttgcca
     1381 atgtttggat aaattgatgg tcatgtgacg caaataagat tgagccagta aatgccatca
     1441 agccgtcatt caatgctgtg attgattcta agtctaaatg gttcgttgga tcatctaata
     1501 caagtacgtt agctttcgaa agcatcattt ttgaaagcat gcaacggact ttctctccac
     1561 cagataaaac attcactggt ttcaatactt cttcaccaga aaacaacatt cttcctaaga
     1621 agcttcttaa gaaggtgtta tcatcttctt ctttacttgc atattgacgc aaccagtcta
     1681 aaatcgttag atcagtatcg aattctttcg ttgtatcctt tggtaaataa gaattacttg
     1741 ttgtcacacc ccaacgtact gttccagtat ctggcgtaat ttcacccatg ataactttga
     1801 atagcgttgt tgttgttata tcattttctg cgacgaaagc gaccttgtct tctttgctca
     1861 aattaaaaga tatatcatct aaaattttct ttccatcaat cgttacactt acattttcta
     1921 cctgcaataa gtcgtttcca atctcacgtt caggtttgaa tccaacgaat ggataacgtc
     1981 tagaagaagg ttggatatcg tctaaagtaa ttttttcaag cattttttta cgtgaagttg
     2041 cttgttttga ttttgatgca ttcgcactaa agcgggcaat aaattcttgg agttctttga
     2101 tttgttcttc ttttttggca tttgcgtttg cttgaagttt agccgctagt tgactagatt
     2161 ccaaccaaaa atcataattt cctacataaa gtttgatttt accaaaatct agatccgcca
     2221 tatgtgtaca tactttgttc aaaaagtgac ggtcatggga tacaacgatc acagtatttt
     2281 caaaattgat caaaaattct tccagccaat tgatcgattg gatatccaga ccgtttgttg
     2341 gttcgtctag caaaagaaca tcaggtttac caaatagtgt ttgtgctaat aacactttta
     2401 ccttttgtcc accagttaat tcactcattt gctgatggtg caattcttca gggatgttca
     2461 gaccttgcag taaaacagct gcttctggtt cagcttccca tccattaagc tcagcaaatt
     2521 ctccttctaa ttcagctgct cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa
     2581 tcgcatcttt ttctttcagc acttcataca gacgcttgtg tcccatgatg actgtttcaa
     2641 gtacagtata gtcttcatag tcgtaatggt tctgtttcaa tacggccatc cgttcatctg
     2701 gtcccatagt aacggaacct gtggaaggct gcagatcccc tgataacact ttaaggaacg
     2761 ttgatttacc tgcaccattt gcaccaatca agccatagca gtttccgggg gtaaatttaa
     2821 tattcacatc atcaaatagt ttgcggtctg aaaagtgcaa actcacatca tttacagtaa
     2881 tcaatttttt tcctcacttt tatcattatt agttttgaca agtcttatag aattatatag
     2941 gggaaaggta aaagattcaa ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg
     3001 aaaaaaataa aaataaaagc ggagcgaaaa tagctactat aggatttatt agtttgcaac
     3061 agcataattt ttgacgttat ttataagagg gagtactata aaactgtaaa agaaatgaaa
     3121 gaaggattga aaatggatga aaaaagaacc atcgactggg gctcattgat tttgggaatt
     3181 ttatttgtac ttgtctcatt gttatcattt caagaccctg tcggaaattt agttgcaatt
     3241 gtagtagtct ttgcgatttt tgcctttatt aaagggatat ttgaactgtt tgtacggaac
     3301 agattaaaag agctaacagg atataaagga aagatgccct taataatcgg gatcatcgat
     3361 attctagttg gcgtcttttt cttattcaat attggtgcag gagtcgcagc attgcctttt
     3421 gtttttgcag tatggtttat tgctgattca gttttagcgt tatttactgc tgacttggca
     3481 agaggagtaa gtgaaggata ttattggttt acgatcatcg tcaatatttt aggaatttta
     3541 ttaggaattt tcttattgtt taatccaatt tcttctgcgt tgacattaag ctttttagtt
     3601 ggattctatt ttatgttatt tggtatcaca catatcgtct atgcatttag ataagtaaaa
     3661 tcaaacaaat aaaaaagccg gcttccgatt acagttttac tgttgtaatt ggaagccggc
     3721 ttttttatca tggacaaata tgcacgaggt gatttttagg tgtcgagaca aatttatgca
     3781 aaaatgtgag aagctatata cacaaatttt atcttaatac tcgaagcata gcgcagttgt
     3841 cacaacctct tttcatataa ttcattcaat actggcaaat catggacttc atatttttct
     3901 ttgaatgctt ggatttcttc ttcagaatat tttcttggat ctaaatgcag atcttctact
     3961 ggcaatgggc gcttgttatt gatcttcaca tcgattacta caggacgatc tgattttgca
     4021 gctgcatcaa aggcaaatcg aagttgcgag tattctgtga ctgtaaagct atttgccccg
     4081 agcgcttctc caactttacc gaaatctgca tcttctaaaa atacaccaaa tttcttttgt
     4141 tccgtatctt cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta
     4201 ataattggaa gctgatattt gacttgtgta atgatatctt gcatgaccat agcaaacccg
     4261 ccatcaccac ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc ggcaattcct
     4321 cctggcacac cattacccat agttgcgaac catccagatg tcgtatgttt ttgtttaccg
     4381 ttcatattca ataaacgaat agaatggata gtcgtgttac cgacatctgt gacgaaaatc
     4441 gcatcgtctt cagcaatacg attgatttct ttatagacag cctctggacg aatcggttct
     4501 tcctctcgat cttcaaaaga tttcaaccaa tttacccatt tttctttatt ttcttgattt
     4561 gctaggtacc agctatcagc agggcgagca tcccctaact cgacaagtct ttgcaaagct
     4621 gtgtttgcat caccgaggat cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca
     4681 tcgatatcga tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag
     4741 tcgctaccga caaataagat cagatcggct tctgccaatg cttcatttcc gggtttagta
     4801 gcgacacgtc cagcaaaacc aagaaagttt tcataatcat caggaataat tccttttgct
     4861 agaacggaag cgacaacagg aactgaaaaa tgttctgaga attctttgat ttgcggaaat
     4921 ccgtttcgcg ttccttgacc aacataaaga actggttttt ttgctttttc aagataaggc
     4981 aaagcagcta gcaaatcttt ttcttctggt aatatcacac ttgttttgtg agtgtgagcg
     5041 gtggagaaag gttgttcgtc aatttcttca aaaccaagat caacagggat agtcacgata
     5101 gccacgcctt tatgctcata ggcggcttta atggcttcat ctactacatg cggaagactt
     5161 tgtggtgtca tgactgtacg attgtatacg ctaacatctg cgaaaatagg attttcattt
     5221 agttcctgga aagagttata gttcatggat gtagaagcaa cttgtcctaa gaacgctact
     5281 agaggcacat ggtccatctg tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt
     5341 cctgcagagc cgaagacagc gccgactttc cccgttaatt ttgcatctgc agcggctgcg
     5401 agtgcgccga cttcttcatg acgaacttga atgtagttga tttcggtttt ttcttgatat
     5461 agcgcatcca taattgaatt gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc
     5521 cagctttcaa gaacttttac cattgcgatt cctgcattga tttttgacat ggtgtttcac
     5581 tcctttagca atgatgatac ataaattttt tgtatctaga gtatagaact ttttatcgaa
     5641 aatcccaact aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag
     5701 tagtacatag atagattcaa gagaggggaa tggcatggtt gaatggctat tgagacttga
     5761 accttggcag caagcacttg ctgggacagc ttttacttat tttatgacag ctcttggtgc
     5821 cgggttggtt tttttcttta aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt
     5881 tgcttctggt gtcatgattg ctgcaagttt ttggtctttg ttagatccgg ctattacaaa
     5941 agcagaagaa aatggagata ttgcttggct agttgtaagt attgggtttg gtttaggcgg
     6001 tgtgttttta tatatggcag ataaaacact gccccatatg cattttggac cacaacatga
     6061 agcagaagga ttgcctaccc atttaaaacg gacgatcttg cttgtttttt ctattacatt
     6121 gcataacatt cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag caactgcgga
     6181 taatccgaca gccgctatat tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa
     6241 cttcccagaa ggagcagctg tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa
     6301 ggcctttgtt tacggacaag cttctggaat cgttgaacca attgctggag tgattggagc
     6361 gctactagta acgaaagtgg agctgctgct tccttatgca cttgcttttg ccgcaggagc
     6421 aatgatttat gtcgtggtgg aagaattgat ccctgaagca cagcagacat tgtccagcaa
     6481 acgtcatttt gcagtttttg gcgtcatgag tggatttatc attatgatga ttttggatgt
     6541 ggcattagga taaataaaaa aaatatatat gatatatcaa atagggccgc gaacttatat
     6601 cgcgacccta tttttttagt aagttatatc ataatgaagt gaatggtatc atacattaat
     6661 aatagttttt tctatgtgta tttcctttga tccagcgaac aagccagatg ataagtgcga
     6721 ttgggattag aactttgacc gcaaaccata aaagcccgct taggacactg acaaaaagac
     6781 tgatgacgaa gctagctgca attagcacta aaagaaccat gaagatattt aagaacatat
     6841 ttttttgacg catacaaaac cctcctcttg ttcattcttt actatagaaa aagtataaca
     6901 gggattgttt aaaaacacct ccaactgaag actgattttt acttcatcta cttaaatgat
     6961 caaacaattg ctttttctct ggtttatcat ctaaaataga gcggaaaagg aggagaatat
     7021 catgcctaaa aatcgtcttg aagcatttac agatgctgta atcgcgatta tcatgactat
     7081 tttagtttta gaacttcatg aacctaacgg agacacgtgg gaggcgctcg ctgcattagg
     7141 ttaccgattt tttatctata ttgtcagttt tatcatttta gcgatttatt ggaacaatca
     7201 tcatcattta tttcaagttg tacataagat aaatggacgg gtcttatggg cgaacagttt
     7261 ctttgttttt gcgctttcgc tttttccgtt tgcaacttca tggatcagtg agtacatcaa
     7321 tagcctggct ccagaaatga cttatggttt tgttaccttg accgctaata ttgcgtatta
     7381 tatcttggca aaagaacttg tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat
     7441 gttgaataat tacaagaaat cgtatctatc gattggtttg aatgtgttag gaattgtctt
     7501 agggtattta gtagcgccag tagccgtgct gatcgcaaac atcgtgattc tagctgcttg
     7561 gatcatccca agtcgtacca tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt
     7621 taagagaaaa atatagaaaa aataaataaa cgatcaaata tccgagtgat actagtattc
     7681 aaagagggat gcagatggat caaaaaagaa ttcatgtagg actgattact agttttgtca
     7741 cgatgcttat ttatttggat caaggtcatt tgacttggac agtagttggt gtcgtaggat
     7801 ttttgacatt attcacagca ataaaaaaat
//
LOCUS       DASJUF010000117         7684 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.119, whole genome shotgun sequence.
ACCESSION   DASJUF010000117 DASJUF010000000
VERSION     DASJUF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.119"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..2305
                     /locus_tag="U4B62_002420"
     CDS             <1..2305
                     /locus_tag="U4B62_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2142548.1"
                     /translation="NKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFTFLGSLSSI
                     YVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQLIDPTEKEI
                     TAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVAVNGSMSAD
                     KTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNTIDSVTPNA
                     DKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKGNVSNFPTY
                     QAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVTGYSFAQTN
                     GSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQDLTTNTPTI
                     NGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKTNATVSINA
                     NGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNEKGVEVTAP
                     TEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGSKTYDGKAG
                     VPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTNSGIDKVKA
                     ANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVTGVVLGESL
                     DYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPSTGGIGLLP
                     FVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2431..2760
                     /locus_tag="U4B62_002421"
     CDS             2431..2760
                     /locus_tag="U4B62_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN2142549.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2800..3804
                     /locus_tag="U4B62_002422"
     CDS             2800..3804
                     /locus_tag="U4B62_002422"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN2142550.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3801..4247
                     /locus_tag="U4B62_002423"
     CDS             3801..4247
                     /locus_tag="U4B62_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN2142551.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4397..5212
                     /locus_tag="U4B62_002424"
     CDS             4397..5212
                     /locus_tag="U4B62_002424"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2142552.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5418..6590
                     /locus_tag="U4B62_002425"
     CDS             5418..6590
                     /locus_tag="U4B62_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2142553.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6752..7246)
                     /locus_tag="U4B62_002426"
     CDS             complement(6752..7246)
                     /locus_tag="U4B62_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142554.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7243..7437)
                     /locus_tag="U4B62_002427"
     CDS             complement(7243..7437)
                     /locus_tag="U4B62_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142555.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2585 a         1448 c         1523 g         2128 t
ORIGIN      
        1 aaataaaatc caacgtatat gtcaaaaaca ttttgtttct agactcaaag ctgtatctaa
       61 aagaaaatac ttacatccca ttatgatttc gatcatcttg atttttactt tcttagggag
      121 cttatcaagt atttatgtaa acgtttatac agaaacgtca aattttgcta accaaacgac
      181 agaggagaca aatagtccac cctattctga aatacagtcc atagaaagca ccaatcagtc
      241 tagcaacaac gagacaacct caaccacatc ctcgactcaa ctgattgatc cgacagaaaa
      301 agaaatcaca gcagcaaaag cagcagggga gaaagcttat tcagaaacag gtagaagcca
      361 gacatttagt actgttgcgg ccactgcgaa tttcaacgga cataccatac cagatggata
      421 tacagtgata tatgccaaag atggagtcat tgttgcggtt aacggatcga tgagtgcgga
      481 taaaactgcg ttacaaggcg taaccaaagc aatcgctttt attgataacc gtccagatat
      541 ccataatctg tttattggct ccacagtaaa tggtactact atcacattcc caaaaagtat
      601 ctatatgggg gtggctaccg ctggaaatac gatcgattca gttacaccca atgcggataa
      661 aagttattct tttacaccct caacatgggg agggcttcct gccggaggag gtatgagtac
      721 tttttcaatc ggtatacagt tagcaaatga tattccattc acggggtact cgatcaaaaa
      781 tgggcagatc gttgatggta agggaaatgt ttcaaatttt ccaacctatc aagcagcaac
      841 ggttcaggtc aatgcgaatc ggacaggaac tttttcttat acagtcaatg gtggagcagc
      901 aaaaatcgga acgtacggga ctacattatc tggaatccat gtgggtgaca aagtggttat
      961 cactccaaca gcagtaacgg gctattcttt tgcacaaaca aacggttctt tcacagccct
     1021 agaaagtaac gtcgatacgg tgacctatac aggcaaacaa acaccaatca ctattaagta
     1081 tcaagactat tttggacaga tgattgctcc ttctaaaata atgaatctta cttacggaag
     1141 tgctgcacaa gacctgacga ctaatacgcc tactatcaac ggatatacct ttacagctgt
     1201 ttcagcgata gaaacaaaga accaatcagc aatgagtgtt ttagctagtt tagatacaaa
     1261 tggaaatgta atcgtaaagg acgcaaatgg aaaacagatt tctgaagtca tcctatatta
     1321 taagacaaat gcaacagtat caatcaatgc aaatggcagt aagtactatg atggtctttc
     1381 tgttgtaccg atcgttaaat atgcctttaa tgataaaaca gaatcaacat cccttttaaa
     1441 caatgatctc agtattgatc aaatcacatg gaatccaact gatttcaaag cgatgaatga
     1501 aaagggagtt gaagtaactg cgcctactga aattggaacc tatacgtctt ggcaattgac
     1561 aaaaagcgga ctagctaaac tagctgccag aacaaactat ttatttacta tcgtgcagac
     1621 atctgatgtc tatacgatta agcaaatcag cggtgcggta gagctcagtg gcagtaagac
     1681 atatgatgga aaagcaggtg tcccgtcaat ccatgtcaag ctagcagagg gtgtcactag
     1741 ttcgcttact ccagtacaag tggcacttag cagtatggat tacacagcag atgcacaatc
     1801 agcaaaaaat atggttaatg ctggcagcta tacaattaaa ctaacgaata gcggaataga
     1861 caaagtcaaa gcagcaaatc ccaagttgtc atttactgat atagccaaca catcaggaac
     1921 ttataccatc tataaagcta acgcagtgat tactgtcgat gacgctgcct ttaattatga
     1981 tgcccaaagt cactcgattt cggttggaaa tctccacgtt accggtgttg tcttaggcga
     2041 atcattggat tataccctaa cagttaacag ccggacagat attggatcac aaatagtaac
     2101 gattagttta ggcgaaggaa gtgtaaacaa taattatacg attatcgcaa cagataccgc
     2161 taagttgacg atcaatccga ctctcttgct tccttcgaca ggtgggatag gtctactgcc
     2221 ctttgtcttt ttaggactga tctttatttt ttctggcttc ttttatttta tccatcgtaa
     2281 gaaaaaagca ggtgaacagc gatgaaaaaa acaaaaaatg tacgtgtgga tatatttgct
     2341 gttctctttt ttatcctact ggtctttttt ataggttctc cgactgtgtc agctagagaa
     2401 tcacctatta catttacttt gcataatcga atgttcgaaa gcgataagat gccaagtatc
     2461 agtcaaaacg acggaataaa tatcccggat aactttcaag gagtaaaagg gttgaacggc
     2521 gttactttcg ctatatatga tgtctcggat gaattttaca agctgcggtc agaaggaagt
     2581 agtgtggaag acgcacaaag aaaactagca caaaaaagtg atagtgaaaa aatactggca
     2641 gaaaatgtga caaaaacagt agacgaggaa gagggcattg cttcttttag tgcatcggac
     2701 aaagatgagc aaggtcgtga cgctgtttac cgatttacgg aaataaagac ttcagattaa
     2761 gtaaaagaaa aatcagcacc atttgtcgtt gtgcttcctg tgacagattc atctaatcat
     2821 aaattgacga ccatccactt gtatccaaag agcgaacaaa aaatacctgc tgcactcaca
     2881 ttaacaaaaa cagtagagaa taagcagaca gattttgcag atggggataa agtcccttat
     2941 ctcatcaccg taacaattcc tgagaacatc aacgagatag gaacctatac gatcaaagat
     3001 acagctgatc ctcagttatg gctagaacca gaaacgatcg attttttgat tggtggagac
     3061 aagattcata catttcacac acaaaaaata aaacatggtt ttgtcttatc agattctgga
     3121 aaagatctta gttcgtttgc tggaaaaaaa ttaacgatcc gttaccaaat gacattgaaa
     3181 gaaaacagtg aaaaaacgac atttgataac gatgtacgtt tgacgactga caatcaaaca
     3241 gtagaaacac atcttgccat ccaaacagga ggcaaacaat ttgtcaaagt ggatcggcaa
     3301 acgaaacaaa aactagcaga tgctcaattt ctagtcaaaa aaaatgacca atacttagcg
     3361 caagaaaacg ggataaatca ttgggtagcc aaagatgatt caagagcaac gatttttact
     3421 tccagtaaag atggaacatt ttctgtccat ggattgtctt ttggaagtta cgaactagtg
     3481 gagattaaac cgcctaaagg atatatattg agtcaatcaa ccattctctt tgaagttgaa
     3541 aaaggcagtt atgatccgct tcatcctata ccattggaaa tcatcaacga accaaaagca
     3601 aatccgacga ataatggaaa aacgccagtt cagatgcaga caaatggtcc atcaaatgaa
     3661 aaaaatggag gaagctttcc aaaaacaagc gaagagatgc ttggaggttt ctcaatttta
     3721 gggttattgc tagtcctttc aacagggaca gcttggtttt ataaaaaaca acaaaaagga
     3781 aacaatagga gggaaataaa atgaaaaaca tcaaaacaaa aatatggacg tttttaggaa
     3841 ctgctatcat gctgcttcct ttcgttctcg gcctaggaac tgcagaggtt tctgcagccg
     3901 tatcacctac accagaaaat gttacggtta atttgcataa attgaaattc acttcagcac
     3961 cagaaaatca aataaacaac ggaacggaat tgacatttcc gaatagcgaa ccgttgaacg
     4021 gcgttgaatt caacgtatat gacatcactg ctacgtacta tccaagtaaa gacacagcag
     4081 ttccagcaga cgctacaccc ttcgcaagtg taacgacttc tggtgaagga ttagctaacc
     4141 tgactcttcc aggtaaatct gatgggaaag atgctgtata cgtctttgta gaaacaccaa
     4201 agcccggtgt cgaaacgtcg ccaaatatcg tgttaagctt accctagacc ctgacaaacc
     4261 tttagacacg attcacctat atgctaaaaa tgtcatcaaa gaaagcaata tcaaaatcca
     4321 gaaagctggg aacttttcag gggtagataa gttagcaggg gcgcaattcc agatcaaaaa
     4381 ttcagagaat caattcgtga ccggttttga tgaaaacact ggtgtcgctc tctatgatcc
     4441 tgctgagtca gcgttgacat tcacaactga tactgaagga tccatcagtg tcaatggatt
     4501 aatagacggc aattatactt tagttgaaac aaaagcacca gatggttatc aattaaccaa
     4561 tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa ttgcaacaaa caaccggtat
     4621 catccatggc gcagatgaag ccaatcttgg gaatccaaac ggtgtagtca atcgggaaaa
     4681 tacaactacg gataacacga ttactgtaga aaatgtccaa aactacggtg atttccactt
     4741 catcaaacaa gatgtgaaca cgcaagaaaa attagccgat gcagaatttt ctattctaag
     4801 agcaaaagat ccaactgtta aattaatgaa aaaaatagca aaccttgcct caggagaata
     4861 cccatatgct tggtcagatg aagtcacagg ttctgactgg gaagaagtga aattgatttc
     4921 tgattcagaa gggaaatttt caatcgaagg gctgaaacaa ggggcatact gccttcaaga
     4981 aacaaaagca ccagaaggct acgcattacc taagaatgaa gctgcgtaca ctgaatttac
     5041 agtagatgat gatccaacaa cttctgatga aacaacgatc gataaccaac ctaaaggaat
     5101 cttgccttca actggtggta tggggatcat cgtattcatc ttggtaggta ctgctttagt
     5161 tggaggagca gtgatctatt tcaaaaaacg ccatcaagaa acagaagctt aaactattgg
     5221 aaacaaaaat catacgataa ataaacgata gtgattcaaa tggggagaaa ggttgtgaca
     5281 agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa taggaggaac tattttgccg
     5341 tttgcttcgc taatactcag gtcaaggcgc tgatttcaca acctcttcat atacatatta
     5401 gaaaataggt ggacatgatg aataaaagca gaataagaat actggacatc ttcatggcaa
     5461 tcattttagt cgttgggatc gggatctttt cttatccttt tgtagaagat tcactgaatg
     5521 attttcttgc ccagcaaatg atcatccatt atcaaaagca agcatcgaat aagaattcag
     5581 aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa ccaacagttg gcagagcaaa
     5641 acgtatcacc agggataggc agtttcaacc aagcagttga tgcaaaagca ttaaaagatt
     5701 tacctagtaa tgcctttttt atggaacata tgcttggagt catcgaaatc ccaaaaatca
     5761 atgtcagctt acctatattc gatcagacaa cggaaatatt tttgcaaaaa ggaacgtcat
     5821 tattggaaga aagttcttat ccgactggcg ggaaaaacac ccatgccgtt ctttctgggc
     5881 atcgtggact cccagaagca aaattattta cagatttgcc taagttgaaa aaaggagacc
     5941 agtttttcat ccagatcaat ggaaagacat tggcgtatca agtagaaaaa atccaagtgg
     6001 tcttacctga tgaagtagat tctttgggta tccaaaaagg cagagatctc gtgactttgc
     6061 ttacctgtac tccttatatg gtcaatacac atcgactgct tgtgacgggg catcgtattc
     6121 cttatcaagc aaaagaggca aaaaaagcga tccaaggaat tgatcaatgg aaaaaatgga
     6181 aattttttat ttggtttatc ggtatactac ttggatcaat cggcttagtt tggctactca
     6241 tcgcttattt agattctttg gccatcgcta agcgaaacta tcctttatct ttttatgtaa
     6301 agaacacaaa cggacgacca atcgaaggaa tggtcttttc tgtaaaaacg ctcaacggca
     6361 aacactatat tactagagaa aaagttccat ttgtgaaagc ttctgatgaa tacggactag
     6421 tgagattttc tgatttaaaa ggcaggaact atcgcttgca acatgaggag ctcctgctta
     6481 aaatccatgt aaaacataaa cattcgaaac agttttcaat gaaattgaaa aaaggaagat
     6541 ataaactgcg aaaagaaaaa gaggtgtact atttgattga gaaagaatga atgaagatgt
     6601 ccaaaaaagc agagggaaca gactaaatga aaaaagagag aagctgacga aatggacgtg
     6661 taaactgtta tttttggttt actatctgga acgccatctt ctattttttt atgtttcaga
     6721 agaattttat tattatagta taacttttga attagtttcc ataacatttg gagcaagggg
     6781 ttaaaccacg agcttgagct tcgcttaaag tggcaggggt atacgtacca tttccacatt
     6841 tatgtgtatg gtattttgaa ccggtcggtg tgacatagac agtttgttca ttattttgct
     6901 caggagctgc ggcttgtgcc tgctgagagg cctgttcagc tgctgattgc tgtcgcgcct
     6961 gttctgcttg ttgctgtgcg attgcttctt gcctcgcctg ttctgcttgt tgctgtgcga
     7021 ttgcttcttg ttgcgcttgt tctgcttgtt gtcgtgcaat ttcttcttgc tttttccgtt
     7081 cttcttctgc ttcttttgct ttgattgtgg aatcaattgc agcaagacgg cttgtcaact
     7141 cttgtttgtt ttcaggtagc gcttggatag ctgctgaagc agttgcatag ttttcttttg
     7201 taggatttgc ttcagcttgt gacaaagcag attccgcttc tgtcactttt tttctagtgc
     7261 agctttttcc gctttttgtt tctcttcttt tttcttttca atttgttctt gttctttcga
     7321 tgaatctgcc gtactttcgg agatcgtggt actgctgctg gaagcctgat ctgaacttgt
     7381 gctttgagta gtagacgtag tagcaccaca cccactcaac aaacttcctg cgaccaaaaa
     7441 caatgcaact aattttttcg ctaaatttcc tccttaagta aaaaacaacc gttttcaata
     7501 aatgtccttt tcaattgttt acttacttgt tataataatt gtgttatgaa tagtgattga
     7561 ggaaacttaa tctaatcccg tgttgataca cgggattttt tgctatagtt ctgttattaa
     7621 gtatgcaata ataaaatatg tacgttattt tattattaga ttattgacat ttatataata
     7681 aacg
//
LOCUS       DASJUF010000118         7575 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.120, whole genome shotgun sequence.
ACCESSION   DASJUF010000118 DASJUF010000000
VERSION     DASJUF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.120"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            183..638
                     /locus_tag="U4B62_002428"
     CDS             183..638
                     /locus_tag="U4B62_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142556.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            757..1815
                     /locus_tag="U4B62_002429"
     CDS             757..1815
                     /locus_tag="U4B62_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEN2142557.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            1857..3806
                     /locus_tag="U4B62_002430"
     CDS             1857..3806
                     /locus_tag="U4B62_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN2142558.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            3809..7450
                     /locus_tag="U4B62_002431"
     CDS             3809..7450
                     /locus_tag="U4B62_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142559.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         2704 a         1127 c         1592 g         2152 t
ORIGIN      
        1 ttattgctaa ttagtctttc atgatgactg aaatagctaa aataaatatc tcgttggata
       61 attgctgata ctttaaggga tatctaaatt tgctgattga tttatttatg ttattatgat
      121 attataataa cataaatgtg ttacgcccga acctcatttt aaaaaaatga aggggtgaga
      181 caatgaatgc tattaattgg catgaatttg ttaactatat ggagagctta ctagatgatc
      241 taaaaagttt tagtattgct gatgcagtaa agcatgggtt accagttagt acaacgatga
      301 aattacgagg attgaaaatt ccagatgaaa ttattaagct gagaactaaa acaattattg
      361 aaaccgcaaa agtcgttgac aatatgaacc atgaactaaa aggcaagaaa acaacttacg
      421 agaaagattt tgaacgtttt ttaaaatctg ttattggaaa atatctaggt aaaaaatctt
      481 tcagtgaatt gagagatgaa gagattgtac ctactccaat ggttagcaaa atccgaaaaa
      541 acaaagccga ggggaattac tatcttggta caatagcaaa aatcattgat tatttggaaa
      601 ataaagagca aaagaaagga acgaagaaag gaagataatt tttgtgttaa atattaatgg
      661 ggaagaatat accgaaatga ttcaaaatcc cgaacctaga attaccgaaa aaacgtctga
      721 ttgtttgctc ttttatgaaa gggggcagac aaactaatgg aaaaaaacgt caattcaaca
      781 tctaccaaag atcaatacag tattattgga gggttgcttg gtgataagga taagagaaca
      841 cgtcacggat tacgcattat gcttgcaact tttctaggag tgcttttttt attaacaggc
      901 ttgggtagct acatttatca attaggaaac tataattatt atctcgtgaa caatacaaca
      961 cctataggct cttcattgga gtttgctcga tcaggagcag agatcactat ttcagatgtg
     1021 tggacagatc aaaacagaga tgtaacagta gtaaaactcg gttactcgca aaacgctaga
     1081 aagttgttat ctacgcaagg aaaaaactac aacttatata tggcaacgaa agaaaaagat
     1141 cgtcctaaaa agcttgaaat gagctatggg atgttaagta ctgatgggga tgctttctta
     1201 ttcctaaaag gtaaattaga tgaaagagct tatcaagttt ttattgcaaa ccaagtatca
     1261 ctaatttctg gtgatgatac agctgacgat gataccaaag tcaatgtgaa tgaagcagat
     1321 tcagttacaa aagcactttc tcaatattct atgaatgatg ttaataatga aggagtaatt
     1381 tcttttggga aaaaagcaaa acctgttaat actcctgata atatcaattt cagaataaat
     1441 ccatattcag attcaactaa tgtctatcaa ggaagctttc ttaccaaaga tggcgatatt
     1501 gattattcaa aagttgtagc agttacaagt aagaataatg ttgtaagtaa gttagaagat
     1561 cagttaaatg cttcaaatac taacatcaag aaaattgaag ctacaaaagc tgagtatctg
     1621 gaacgtttac aacaaaatcc tgacgataaa gattcgcaag aaagtattcg tcaactagac
     1681 agtcgaatgg agaccgaaat taaagcaatc aatgatacac aaaaattgat tgatcaatac
     1741 aaaaatgctg actttaacaa agattctttt ggagacatga agacgacatt taagtaccta
     1801 actctgactg actagaagta aattattctt attttagagg aagtgaacac aaacagatgg
     1861 aaaatagtac cgagtttttt aagaatattc aaactgaatt taatcatgct ctcacaccaa
     1921 tgaaaccacc gaaacataag ttagtgggac gtgatgacga actccatgag ttgtcactga
     1981 tcttgaatag accggaaaca ccgcaagcta tcttgttagg tgaagccgga acaggtaaaa
     2041 cagcacttgc aagtgaatgg gctgaaagac agcgacaaga tggaaagaaa gtacaagtat
     2101 acaaattatc aatcggacaa ttgcgtgttg ggggagatga tgtcctccaa acacgtttag
     2161 aaacattaat tccaagattg aaagaatatg agcgaatttt aaaatctgag gacaaagaaa
     2221 atgaatttgt cctcttcatt gacgagtttc ataaagttgt tagctctttt ggtagtggca
     2281 ataaaattgg gggtgattcg ctaaaagaac cgttagcaaa tgaatcactt caattgatta
     2341 ccgctactac aagaaaagaa tatgaaaact atatcaaaag agatgatgcc ttagggagac
     2401 ggttacaacc gttagaattg aatgaactaa aaagcgatgt tgtcgtcgat attttgagac
     2461 aatggctaaa tacatcctct gttaaagatg atcctcaggc ttcttttaaa ttatcgcaaa
     2521 ggattcctga tgaattactt caagaaatcg tacaagcaaa caagctttat cgtgaggatt
     2581 tgtccgaacc ggctaaatca attgccgtaa tggaggattt gattgcaaaa gatcgagtag
     2641 acggaaatgc ttataatcga gaaagtctat tgcaaatatt tggtaagtat aatattcagc
     2701 tagattctga ttttgatatg gaaaaacttc aagaggaatt aaagtccata aaagggcaac
     2761 cgttagcagt ttatactata acaatgttgt ttaaacgtat gaagcttgga tttgatcgtt
     2821 ttaaaggtcg aaagcaacca aaagcaactg ctctgtttac aggatcaaca ggggtcggta
     2881 aaactgcaat ggctaaagcc gtggcaagag gactttatgg cgatgaaaat aaagttctct
     2941 tagttaatat gccggactac aaaacaaaag atgatgcaat gcgttttaga cgactcatag
     3001 gtactcatgt aaggcatgat ccttctgcga ttattgtgtg tgatgaatat gagaaagctg
     3061 gtgtagaaac actcgatgcc tttttatcaa ttttagatga aggtaggata agttttactg
     3121 aaagaggatg ggatgatcga gaagttcacg acactgtttc attgtcaaat acaattttat
     3181 ttgcgacaac aaatgcaggt gcggagatgt tcgattcgtt aaacagatat agctcactat
     3241 ctcgtgttac aaacaaggaa aattctaatg aatatagcac tgaactaaaa agagaatacc
     3301 gaaaagtcgc atcagggatc aaacaagcct tgattggtga aggtctaaag cctgaagtta
     3361 ttaaccgatt tggacgaata gttccatttt gggctttatc cgaacaaact cttctggaaa
     3421 ttgcttataa tgaaatgttg aagagtgctg aattagtacg aaaacattta aatatagatt
     3481 tacgtcttcc acctaggaaa gaaagagtat atggtcaaaa tttttatgca gacgatctct
     3541 ccatgcactt agtttttgac atggcttcac aagaggattc taaatcaggg ggtgcaagga
     3601 atatcttgca tttagttgaa tctgagtttg aagaagcgat tactgaagca ataattactt
     3661 atccaaacgc aaaaatattc gatgttaagt tgagtgaagc cggagcgttt gaaggaaaag
     3721 gtgattctaa tgctgaaatt attgcaaagc cttccgttgc tgaaagtaaa gaaaacaaga
     3781 ttgtattttc gattggaggg gcgtagctat gaaattcaag aaatatttac tacaaaacct
     3841 tagaataaaa cgatgggtcg taagaggtct agttattgga ggagtattag cagttacttt
     3901 aggcgctcct tttggaatga atgatccaac tcctgacaat caagatagca aacaagaaac
     3961 agttataaat aagaacgttg catatgcgga aagtaaaccg gcagaacaag gaatgtggac
     4021 actatatgct cgtatcctaa tgggagataa agaagttaat gatagtgccg ctaaaactgg
     4081 acaaatgaat ttgttaggac aaggtggtat cagtgccgat ttcccttatt ctgacatttc
     4141 taaacaaggt aattcgattg cagggggtaa tgcaggtact caactagcat ctactttagc
     4201 aacttatagt tactacggat atattgaaac agtctcagga aatgctattg ccagtcaagc
     4261 atcatccatt ctaggaagta ttggtaggac gattggtagt tttatcggat caattagttt
     4321 aatcctttct aatattgctg aatccataaa tgaactcctt ggaaaatttt tcatgagtat
     4381 gaatatattt tctcttatgg ggtggggaga aggtggatcg gatgtatcta atccggtatc
     4441 taaagcatta actagcttgc ttaacaattt gggattaaat aatgaattat ttaaagcgat
     4501 agcaagctta tctatgctct ttttcacagc aatactaggt tatcaaggaa tgaaattatt
     4561 gtcgaaagat ggtgttaact cgaagggatt atcaccagta cttgaaaaat ggattgttcg
     4621 tatttttgta ttgtttatat tcttaccagt ggcaggatcg ctattgggag tttatatcaa
     4681 cggcatccag tcagcaaaaa aagatggaga tataccaaca aatgttgttt ctgactacat
     4741 cattaacaca agactttggg cttctactca aaatcttgct ccatcatctg atactggtgg
     4801 tgatgtacct gacggaaagc ctacatccga aatcaagttc gttgatacag attatgaacc
     4861 atctaaaaaa tctgcaatga taagagcgat caatactagc agttatgcca gacaaaataa
     4921 tgatctagat tctaattcag tcggtatggc tctattaaaa gaatggtcat cgaatgatac
     4981 tttcaatatt aatacttatg ccggtgatgt taatgtcgcc gaaaatgatg cttatatggg
     5041 aagagcgcct gctgtttgga caggctataa tttaggggat ggaaaagaag gaagtgcaat
     5101 taagaaacca accaatttaa atggatatat ttggtcagca aatcaaaatg tgacagacga
     5161 caatcgcaag cctgatggaa aaggaacagc gcataacttt gatgctactc aaccttgggg
     5221 cgttgctaac aatgcaactt ttagcacaca gtcggtagct ttattgctcc aatcggcact
     5281 tgatacatcg ggagctaagt tttatgcgta taatatcgct ccttcaggaa ttcaaggcag
     5341 tgctaagaat atgtcaacag taaaaacaga atggcgtaca gcatccttag ttggtcatgg
     5401 agctatgggt aagtttggct cgtggctagc gatgaacgct gaatcaatcg ttttagcagt
     5461 tctttactta gcatgtgctt gggcgatgtt caatgtgaat atcgtgaaag catttaaacg
     5521 tgaatttatt cggatttggg aagtagttct aacaggatca cttgcatcag gagcaggtgt
     5581 gatggcaatt tcattaggtg ttgttacaac atctctgatc ggactacaat tacctatctt
     5641 tctgattact tttattcgtg catttggtga tactgctgtg ggattcattc ctaatacatt
     5701 agttggcgat ggcgtagcaa atattgttcg atctgctatg attttaggtg taggatatct
     5761 tttagtcttt aaaaaaggcg aaaaagtaaa taatttgatt aatatgctta ttggcttacc
     5821 acttgaagta gcgtttgggt ttgatgaacg tgctaagtta ttatttggta atagaaatga
     5881 tgctgtgcga gttgtggacg gcgtttctaa agcaatcaag aaaagccaag gagctaaaaa
     5941 tccctattat gatggtggca acagctcagt aaaagatatg gttgcaggag gtcttggagc
     6001 agttggagcg caaaaaatgg ctgaacgttt gaacaatatg ggagatagca atgctgatgc
     6061 taagtccatt gctgataaag taattcctga aacaccagaa ggaatgtcat tcaacggtgg
     6121 cggagttggt tccacaatca ataatacctc tttcaaagat ttagttcgtc aagatcaact
     6181 acgtaataac tcccaacaat atattgatca tttaggacgt atggttgatg aaaacggaaa
     6241 ctttgtcgat gaaaatggtg aaatattaaa agatagtaaa tacggtaaag atgctaaagg
     6301 caactttatt gacgaaaaag ggcgtttggt cgatgaagat ggaaacctta tagatactaa
     6361 tggtaaacca ttggaagata gtgaagatgg taatattggt atggatagcg aaggacgttt
     6421 aattgatgaa aaaggtcgct tagtcgatga agatggaaat cttgttgacg aaaatggacg
     6481 caggttgcta aatggtgatt ttggtcatga tagtaacgga cgtttagttg acgaaaaagg
     6541 tcgtttagtt gatgaagatg gaaatcttgt tgatgagttt ggtaataaac ttggtaaaca
     6601 cagcgatgga tataatgtga atggagattt agtcgatgag gatgggaata tcattgagac
     6661 gagagatgcg cttgtggcag atcgaaatag agaactcgaa caacaacggc atacgccaat
     6721 tgctgttgca attcctgaga atgaagaaga cgagattact ttagatcaaa ctcatcaaga
     6781 actgaggaat gaaatgagtg gtgaggacgt gtcagacaga gtgcatcggg aattaggaga
     6841 tggagcatct ggtgaaagtc agtcaagaga gcttaacagc aactttgata atagtgctat
     6901 tcggaatgac acagtactta aagacagaga atcgctaaca aaagaaacgg ataaagaaca
     6961 atcagatgtg aagaaaaagt tcattcctac tcctaatgtt aatcaaacag ataaatatga
     7021 aacaaatatt ggatcaggaa tgtcgaaaga caatcaatca attagagaac ctgaactgac
     7081 aaataaaaat aatagaacaa ataattctaa atctacttca gataattcag ggaaatctaa
     7141 tcaatcaacc aagaaaacat caacagctaa atcagccgtt gaatatctat caaaaactgg
     7201 taaaaatgtt tacacctcag cagataaagc tttagcaaaa gctccaaaag gtagtctagc
     7261 agggataacc tatgaagcta aaaacgaagc tattaaaata ggtaaaggaa cggctaaggt
     7321 tgccaaggct actgctacag gagcatccaa aactggtaaa gtagtgaaga aatctaccat
     7381 gtcagtcaag gactatattg taaaatacgg taatcaaaaa tcaactgaaa ataagtctga
     7441 agacgagtaa aaagaaggag cattgatttg ctccttcttt ttactttgat ttctaactga
     7501 agatgtcaag gaaaatttct atttatgcaa aaaaattatt ttaaattgtc tataaatgaa
     7561 ttaaatattt actgt
//
LOCUS       DASJUF010000119         7537 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJUF010000119 DASJUF010000000
VERSION     DASJUF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.121"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..1248
                     /locus_tag="U4B62_002432"
     CDS             join(107..335,335..1248)
                     /locus_tag="U4B62_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2142560.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="U4B62_002433"
     CDS             1483..1935
                     /locus_tag="U4B62_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142561.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="U4B62_002434"
     CDS             1949..2638
                     /locus_tag="U4B62_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142562.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="U4B62_002435"
     CDS             2763..5114
                     /locus_tag="U4B62_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN2142563.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="U4B62_002436"
     CDS             5194..5589
                     /locus_tag="U4B62_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142564.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="U4B62_002437"
     CDS             5914..6531
                     /locus_tag="U4B62_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2142565.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="U4B62_002438"
     CDS             6572..6871
                     /locus_tag="U4B62_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN2142566.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="U4B62_002439"
     CDS             6905..7072
                     /locus_tag="U4B62_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2142567.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="U4B62_002440"
     CDS             complement(7117..>7537)
                     /locus_tag="U4B62_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2142568.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DASJUF010000120         7283 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJUF010000120 DASJUF010000000
VERSION     DASJUF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.122"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1510)
                     /locus_tag="U4B62_002441"
     CDS             complement(<1..1510)
                     /locus_tag="U4B62_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142569.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNH"
     gene            complement(1530..1697)
                     /locus_tag="U4B62_002442"
     CDS             complement(1530..1697)
                     /locus_tag="U4B62_002442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142570.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            complement(1734..2423)
                     /locus_tag="U4B62_002443"
     CDS             complement(1734..2423)
                     /locus_tag="U4B62_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEN2142571.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(2678..2998)
                     /locus_tag="U4B62_002444"
     CDS             complement(2678..2998)
                     /locus_tag="U4B62_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142572.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3031..4149)
                     /locus_tag="U4B62_002445"
     CDS             complement(3031..4149)
                     /locus_tag="U4B62_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142573.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4178..4972)
                     /locus_tag="U4B62_002446"
     CDS             complement(4178..4972)
                     /locus_tag="U4B62_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142574.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(5025..5378)
                     /locus_tag="U4B62_002447"
     CDS             complement(5025..5378)
                     /locus_tag="U4B62_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142575.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            complement(5480..5737)
                     /locus_tag="U4B62_002448"
     CDS             complement(5480..5737)
                     /locus_tag="U4B62_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142576.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            complement(5740..5964)
                     /locus_tag="U4B62_002449"
     CDS             complement(5740..5964)
                     /locus_tag="U4B62_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142577.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(6137..7180)
                     /locus_tag="U4B62_002450"
     CDS             complement(6137..7180)
                     /locus_tag="U4B62_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2142578.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2022 a         1525 c          910 g         2826 t
ORIGIN      
        1 catgattcaa gtaatctgta tatagaaaaa tcgcatcaca cgtattcaaa tttttaagta
       61 tctttccatt attttcgctt acatcaaaca ttttaatatt tggtaatagt tcagttaatt
      121 tattttgaaa atttacatgt cctcccacta aaagaatgtt tttatgtttt agataagcta
      181 tcatttcaga cagtgaaggc tcttcagttt taccatttaa tgattgtcta tgttcttgct
      241 cctgcaattg ctttaatact ttattttcaa acctcagctg ttcaagttct tttctgattc
      301 cttttaattc tttaatctct tgcttcaaaa gttcctcttc tttttttgcc gatttgacta
      361 aagattctaa ccgaccgttt tctttactcg caatacttaa ttcttttctt aatttgatta
      421 cttcagattt gttttctttt ctaatacttg caatttgttt tgctatttca taatcagaat
      481 tcaggatcaa ttcttcctta gcatttttaa acaaccgagt taaaacgaac tgatataaca
      541 aactataaaa aactagcaaa tagtcaattt gtcggtactc ggtattctga tcaattgcat
      601 atagagagat taaaacgaaa tacacaatat cattcagctc gctacccttg atctcaaaat
      661 tattgactaa gtaactatca atacccatca aatctgcaaa atctccatac aaagaaaaat
      721 atgattttga caatatatct atatctgaat cgtcaaaccc atttactatt ttcccatcat
      781 tgttagtagt tgctaacgaa gcaatagtga cttttgaatt gggttttaca aatcctatac
      841 ctaatagttc tttcatcttt ttaatttcaa gtttgttatc cttttgttcg gctttatgct
      901 gtttcatatt gtaaagcgga taaaatgctg ttccgtaagc accttttagt acgttatcta
      961 taaaataatc ttcagaacct gttttgaata gaaagttaat cgcagccaaa ttagataaca
     1021 gctcttcagt gttgtgcatc aattttttat catttaataa attatcccca agatatttca
     1081 ttgcataata agggtttgtt tcgctactta tttttaatat tttttgatag ctattcctca
     1141 cgatattaac caaaatatct gtctgattaa ttgaattaca gtacaaagcc actcctaatg
     1201 ccatataaac attcaaatca tgttttgccg taccgttgaa taaaacgtct tcaacttttg
     1261 aattatctcg tctatacttt ttgatcatat ttttgatatt ttgatcattc tcttggtata
     1321 tttttttcac tcgttccaat gccttgggtt tgttggaaac agcaacagcg aagtctagtt
     1381 ctttgaaatc aatatttagt atctgtacct catcacctag attataatct ctatttacgc
     1441 tcggaaaact gctgaaatct aactgttttt tacctatttt tggcgaaaat attccttcga
     1501 tttctggcaa atgtaatcac actctctcct tatttatctt tagaaaattt gtcactattc
     1561 tccttatatt tatcaataaa cctttttaat cgagcaatat aagcgttaac ccttttaggt
     1621 tcttcttttc gccattcatc gtttacgtga ccattttcaa agaaagaagc taaaagagtt
     1681 tccacttcat taaccatatc tgcgatcgta tatttttctg taccctcact caattattca
     1741 tgaactgtta agtgttcata gctaagttca ttcataattt tgttatagcg ttttcttgct
     1801 ttttcaaatt ctttctgttc gtcaaattcc acttgattct tcatgttgtt cttcctctct
     1861 gtttgatatt ttttcacttc ttcaagttta gcatctagtt ccgctttaac ttctgtcact
     1921 tctttaagcg cttgggataa cttatgagac aatttcgcat aagtttctct cttgtattca
     1981 tttcctactc ccaactcttc ggagtagaac tcttctagta gtcttgcaaa attaacattt
     2041 ctcctgccag catagttttt tagcttctca aaatcagatt gaaagacagc aacacttttg
     2101 taatcctttt caccctcaaa atcaaaagca tagtcatact ttcctgaaat caattcatca
     2161 ataactgtag caatatattg agagatagaa atattatctt cacttctatg tcgctctata
     2221 agattatata tatcagggct gacatgaaac gtacttctct tttttgtttg cctttttcta
     2281 actgttacga ccacttttgg aacttcacta tctccattga tctctttgta tttttcaata
     2341 aataattttc tagcttgttc tcgacttttt gccggaattt catctacaat tttatcttct
     2401 aaataaatat agtgtgttct catacattcg tcctctatct tctttactct caatcttcat
     2461 gatcttagtt cttaccttgt ttactcttaa acctttcaaa aaaatctagt caaaatcaaa
     2521 cttgaaatca tcgtctatca caacaccctt aacttcatct ttagatttcc caaaggaacc
     2581 cttattttca aatcctttat ttttgaatga tgatggttat ggaaaacttc ctttgaaatt
     2641 ctgacaagca taactagtca gaactgtttt acttagatta agctttcaaa agatactgat
     2701 gaagaactaa gttctcagta ccttcccatt cttcaataaa aaatatttct ccttctttta
     2761 cccactctat agctaatttc ttataatcaa ataaaccata tgtgtttaag cctttgtctt
     2821 tcattaaact caaaaactga tcccctttta atctttctgt atcatccatc agaaattcaa
     2881 ctaaatcttt atccatgcgt gcaacttttt cgtcttttag tgccattgat ttagcatagt
     2941 cagttgaatc ttttagaata ataacaccta catttccttc cacaattttc ttcttcatat
     3001 ttacctccta ccatcttttc caaagagtat ttagtcaaaa tcaaactcga aatcatcgtc
     3061 tatcacaaca cccttaactt catctttaga tttcccaaag gagcccttat tttcaaatcc
     3121 tttgttttta aattctaaag ctttttcgac ttcttctttg gatattgtat gaactttttt
     3181 cttaactact ccaagtttat acagctcttc aaactctggt acttcggatc tgaaatacaa
     3241 tccatcagtc cgaattttta atagcccata tgcgctatga acgtatttct gttcatatct
     3301 aaccatacga tctctaaata cgcctgctac catggaggaa tacgctcgat tatacatact
     3361 gtatttatcg aaggtgcttg tttctccttc tcttaatgat ttttgtccga tagcaccaaa
     3421 tactttcgtt ctaagatttt tataaagctt caacgtatca ggatcagttt ttaatggctc
     3481 gattctttca aaatattctt tgagtaatcc ttttttcgtc aaaaatgtgt ggctatctag
     3541 gacttccatc tctttaatat cataataatc aaggataagt gttagctcaa tctccgataa
     3601 ccaaataggc tcattagctg ttttgggatt ttcgccataa tccgcaaaca tcgactgtga
     3661 ttttggatta gcatatgttc cttctttggc tgttccttca aaattaacca aaaagaacat
     3721 atacgttctt aaattttcat acggtcttac ttttggtcta tttatcgctc tcaaaatagc
     3781 ttgatctgta ttatattttg ctttcaaata agtgtttgac ggcaaaggat aatttctcat
     3841 gatcctcgaa taggcttcgt tgaaatcata ttcgatcatc tttgctcctt cctcaaacaa
     3901 atccaagtta tacatgctac cggaggtact ggctctatag gctgaacttg gaacaaagaa
     3961 aggtgcatct tccggcttat catagcccaa aaaatgtatt cctgtttgct tgttctgacg
     4021 gtaaatgtcc atcaaaagat atttgatcgt actaaaagaa tctaattttc ctcttgtcat
     4081 ccaccctaat ccgacaagtc ttttaatttt attctgcatt tcaggtgtgt atatgctatt
     4141 gtttcccatt tatctgctca tctccggctt ttttattcta gaccctatat ttgttcatta
     4201 caccacttat ctcgtctaca aaggctccca gatcgtgtgt aaaggcatcg tagtaatctt
     4261 caatctcact cgattcagtt ctccggtttc ttccgttttt atttctgaat agatacatct
     4321 gcgtttctct taaatccctt gcttttttcg caagtgctaa taaatccgta aaattatcaa
     4381 taaccataat gtcattgatc ataaaacctt cttcatgacc atacttttca gccatctcga
     4441 taaaatcatt aacatcgaac aactccaata attctgcttt atatttctct aacaatgtat
     4501 ttaaatagcg atttctcaat tcccatgaac tttcaccagt tccgtattca accttgatcc
     4561 ctaaattctt agtgtagcct tttgctgtgt atcgtccaat gaagtctccc ttattccgat
     4621 tgctatcttc ttccttgaaa aactcacgcc aaattccctt tttcttttta ggaatcagac
     4681 ctctgctctt gaaatcatta tatttttttg aagcttccct tcgtgtatct aagtaccttt
     4741 gcttcagctc tgtagctgat tcattcgttc catattcaac agtgatcctt aatcgtttgc
     4801 tatagccttt tgctgtatat cgtccaacaa agtcaccgcc acttcgatca ctactgactt
     4861 ccttaacaaa ttctctccat ataccttttt tgtctttcgg tattaatcct cgtcttttca
     4921 tatcttgata tttttcagaa gcttctttta gagtattctt ttttgctacc atgtcatcac
     4981 ctcgcaattt agttaacttt atacacgtct acaaaaaaat atgattactt actttttaaa
     5041 ttgaaaaact cgttgaaatg ctcttctttc aacacaacaa aagcatgata acttaacctt
     5101 aaaatataat ctccatattc aactggataa tctttggtta taactctaat taccgctctt
     5161 ccgctattta caagttcact ttttttcttc ccccttcgta ctcttcgtat actatgaggt
     5221 tttacaaatt cgactacttg cttccaatta tcatttttat attgaataca cagtggtgat
     5281 tttttcattt ctatatacat gttcttataa tcttctactt tcttattttc tttaactaga
     5341 tgtttattat tctcataaga caaattttcg cttatcattt aatatttccc ttcctttttt
     5401 tgcattatat ttagcaaaca ttcaccattt gaatttgaca tagctttaag acagatattt
     5461 tatcttttca aacattaagt tatttgtctt tttcagaagt ggtataggat cgaagtcctt
     5521 cacgtttcat tatgtcttct gcttttccaa tgctgatccc tttccaatca cgccatccta
     5581 cgcttccctt attttgacga taggctttaa tagctgattc ggatatttgc catcttgtag
     5641 ccagctcttt tgcggtgtac ttatttgaga aaagtaactt ctctatcagt gaaatatcaa
     5701 tcattttatt ctcactcctg aaatcttcat taatcatctc tagctagcta tatcttcaat
     5761 caattctaat atgtcggata aaccatcaac agcttcttca agttgatcaa cggattcttc
     5821 tgctttttca tatctttctg atccctgtag atttttaggt atattttctt tgtactcttc
     5881 ttcttgaatt acttctaact catcctttag ctcggaaagt cgctcgctta tttcttctaa
     5941 cctttttctt cttctttggt tcatcaaaat cacctcttcg cataatctct acgattttaa
     6001 tataccactt ttgtgtacta cattcaatat tttttgttat agattcgtat tacaaaaatt
     6061 tcctctaaaa aaataagagc ctatttacca tttcagtaat taagctctta tttcatttag
     6121 ttgataattc tatcaattaa cgtacagcaa ccgccattcc agtcacatta aatgctctct
     6181 catacgtgct tgcattgatc cattttgcgc caaatacagg gtcaacaaca tggtatgcac
     6241 catttctgaa gccatccacg agaactacat gtccattcca tactgcacga ccccaaggca
     6301 tttgtttgaa ctctggcgta gcatagttat aagtaatcca agttacaacc ggattaccat
     6361 tgcgaatttc attcttgatt ccattcgttc ctgaaccagt gatattgacg gcattaccgc
     6421 caacgttttt tgcccattta actactgggt cagccatcat gccttgatac gttccattaa
     6481 cgttatgacg ccactctcct gagtaaccat gataaggatt attgtcatta gcttttggca
     6541 tatagtttac aaaactggtt aagctttgat ttgtggctac acctttgtag tgtaaacctt
     6601 ctaataaaga agctccttcg cataacattg tatttccttg ccatacaaca ggaacgttca
     6661 acactttttc agtgcttgga gcaggtgtag gatttggtac gctgtttggc attgcatacc
     6721 agcctgtacc ttcaatcttc caaccttgtt tactaattac gttcttttca ttttgatcta
     6781 acgtgtagtg atgccaattc acgtatttgt tatataaacg tgttacagga actctatttg
     6841 ctccgttgta ggaatagaac gcaacacctt cgtacgtcca tcctgtgtgt gaaatcttat
     6901 ctctttctcc ggcatcaact gtgtaaaaat gttcaccgtt ataccgatta tacaaacgat
     6961 aaacaggagt accacttgtt ggagcttgcc atgcgacacc ttcgtaatgc catccataac
     7021 gtttcagatt gtctctttct ccaacgcttc tagtgtatag atgctctccg ctattgggat
     7081 tatacatacg atacatggaa atagagcttt ctgctgaaac tgaaggtgat aataatacta
     7141 gccccattgt gaatagaatt cctactaaaa attttttcat ttgattttac cccttttcat
     7201 tttttattgt cattattgca tgaaaattat tcgtaagatt gccaattaca taaacgattg
     7261 ttttaagatc ataatcgttg ttg
//
LOCUS       DASJUF010000121         7248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJUF010000121 DASJUF010000000
VERSION     DASJUF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.123"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1463)
                     /locus_tag="U4B62_002451"
     CDS             complement(join(37..890,890..1463))
                     /locus_tag="U4B62_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HEN2142579.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSPVQFRKTTVTVV"
     gene            1706..2161
                     /gene="tnpA"
                     /locus_tag="U4B62_002452"
     CDS             1706..2161
                     /gene="tnpA"
                     /locus_tag="U4B62_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN2142580.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2426..2524
                     /locus_tag="U4B62_002453"
                     /pseudo
     CDS             2426..2524
                     /locus_tag="U4B62_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2747..2977)
                     /locus_tag="U4B62_002454"
     CDS             complement(2747..2977)
                     /locus_tag="U4B62_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEN2142581.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3207..4025)
                     /locus_tag="U4B62_002455"
     CDS             complement(3207..4025)
                     /locus_tag="U4B62_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2142582.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4186..4875
                     /locus_tag="U4B62_002456"
     CDS             4186..4875
                     /locus_tag="U4B62_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2142583.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4889..6391
                     /locus_tag="U4B62_002457"
     CDS             4889..6391
                     /locus_tag="U4B62_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2142584.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6404..6880
                     /locus_tag="U4B62_002458"
     CDS             6404..6880
                     /locus_tag="U4B62_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2142585.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6977..7087)
                     /locus_tag="U4B62_002459"
                     /pseudo
     CDS             complement(6977..7087)
                     /locus_tag="U4B62_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2342 a         1149 c         1308 g         2449 t
ORIGIN      
        1 aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta cggttgtttt
       61 acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta
      121 atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg
      181 aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt
      241 ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt
      301 catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt
      361 ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct tttcagagat
      421 acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat acaatttctt
      481 ttgacggata atgcctttgg catccacttc atggtattta aattcagttg tatccgtcgt
      541 gattttctga tggcagatac tggtgtagaa acgacgatgg atccgatttt tggctactgc
      601 tccgacttgt cctcgataag aattgtagcg ccgtgattta cgtgtgtatg tcgttacttt
      661 tattcctagc ttcttgatca ggcgctgaac tttcttttta ttgatagaga aaccgcggtt
      721 tcttagttca ttggtcatcc gtaaacagcc ataatccttg tgcttctccc gaactttaag
      781 catttccgct tcaatctctt gatctggatt tttacggtcc aatcgttttt gccagtacat
      841 aaagattgcc ttagggaact ttaaagtttc tagaagctca actaatttaa acggtcctcg
      901 gaggcttgcg atgattcgtg cgattgcttc attcgtcgtt gttgggcttc ctgttttcgc
      961 agcttcctca attcttttaa aaaggcattt tcaatttgaa gggaacggac tcgtttctcc
     1021 aactcctcga tgcgttcacg ttctttagac gttgctttag ttgtagattt tttcttattt
     1081 ttgggttcat ttttcttaga caagataggt ggacatcctt tcgtttttga gagtccatcg
     1141 atcccaccct cacggaattg acgcatccaa ctagcgatca aattagggtt gttcatgtta
     1201 agtgtgttgg caacttcacg ataggacagc tcacttgtta gatataactc tatcgcatcc
     1261 agcttaaatt gaacagaata ttttttcttt tgtcgctttc ttactagacc ctcttcacca
     1321 aattcttgat aggcattgat ccagcgcttc acttgtgatg gagccttaat ggaatatttc
     1381 tttgccagat aatcacttcc gcctttacca tccaagtaat catgaacgat ctttaattta
     1441 agttcaaaag tatacttagc cataaaaaat accgacctcc aaaagttaga ttttttggtc
     1501 taacttttgg gggtcggtac aaaacttaaa agttttagac ccttttattt tcttaatgac
     1561 ttcagtcagt tttgtgcgta aagcgtgcga aacaagaaaa ccgcgcgttt ctacgcgtgg
     1621 aagaagtatc tttagcgata aaaactcctt tcgatatact agtgagtggc taccaaccgc
     1681 tcatcagatc gaaaggagtt ttttcatgtc aaatgacgat aaaagtttag catatactcg
     1741 ttggaattgt aagtatcata ttatttttac accaaaatat caacgaaaag taatctatgg
     1801 gcaattacga aaagatcttg gaaaaatact taggaagttg tgcgaaatga aagatgtgga
     1861 aatcatagaa gcacatgcaa tgctagatca tattcacatg cttgttcgag taccaccaaa
     1921 aatatgtata tccagtttta tggggtatct gaaaggtcgt agtgcggtac taatccacga
     1981 gcagcacgca aatctcaaat ataaatacgg aaacaaatcc ttttggtcga agagatacta
     2041 tgtgagtaca gtcggattga atcaaaagac aaatgaaaaa tacatccgtg aacaagaagc
     2101 tgatgatcgc attcgatata ttataagtaa aagagagtac gtagaaccat ttaaaaaata
     2161 aagagaagta agtgagtgaa gatgagcagt tggcggtctt ttaaaaatcc ccttttaagg
     2221 ggcagcgagt gtcgacccct tctaggggga ttaaaaatac acccgtcctc acgggtgtat
     2281 gggttactga tagacttgat tttaactggt gtcagaaact tatgtccaga aagggtgatg
     2341 aaattttggc aatttacggc tattcaagaa ttagcacaag caaccaagat tacaaaacac
     2401 aaattcaaac attagaaaat gctggcgcag aaataatctt cctagaaaat cactggatta
     2461 tcgaacacct ttggaagtat ttttgagtta cgtaagtatt gatgatctgt ctaacttaat
     2521 ttgacaatta agagtatatt tacttcagat ggtaaggcaa caaaataaaa tacaataggc
     2581 agatgaacta ataaataaaa tctgtattaa atacagataa attaatataa aaatcaaaag
     2641 gatcataaac ttttaacaag tgatgaaatg ggtggaaatt gattcttata gacagtttgt
     2701 ctcataacat tgaagtattt tgcaatttta gcattgaaac acttttttaa tcttcaacgt
     2761 tgttcttata aacaagatga ttgggaatct tttgaattaa cactgtatag cttagaacac
     2821 cctttataga aaacattcgc ttagcaatct taataattcc ttgtgcccga tgtcgtttag
     2881 attcagtagg ttcctgctca tcgatcatgg aaaaaatttg aaggatcaga tccttaataa
     2941 atttatttaa gagaggatga tccattgctt catccatact cgataaacta gtgataatga
     3001 gctacacgtt tttctttcaa gttgatcaat agcttccacg ataaaaatat ctttagataa
     3061 aatcaagaac cgtaggaact acagggatat ttggtcaata agagccaccg ctattagtaa
     3121 aaaataagct atcaagtatg ctctcatccc aagaattgcc tacttcaatc gttaaaaacc
     3181 gttaggttta gctaagtggg ggtagttcac tcaatacttt tatcaataat agagcgcaaa
     3241 actttttctc ttaattcaac tgaatcaata ttattgtttt gttcataacc atgaactaat
     3301 agatcacaaa tataaagttg tgatattttt ccagctaaag atccaccatt aagaaattct
     3361 tcaatggctg tttgtaaaac taagtctgca gactttccta taggtgaatg aatatagtta
     3421 gttatagcaa taatttttgc gccatttttt ttagctattt tcaatgaatc ataagtatct
     3481 ttcgttttcc cagataaaga aaaaatgatt actaaatcat taactgttaa taaagaagcg
     3541 acttgggctt gaaaatgagg gtctagtaca gcttttgctt gaactcctac gcgaagaaac
     3601 atattttcta aatcaagact agtatttcca ctagatccca ctccaaatat atatatgttt
     3661 tttgattgag tgatcatttt aatggcttca ttcaattttt cttcgtctaa aagcctaata
     3721 gtggaataga gagcttctgt taaactatta gcaatttcat catagtactt tacactagtc
     3781 ggatgttctt ttttttgact aaagtcttcc tttgcaattt caatttttaa atcagaaaaa
     3841 ccagaaaaac caattttttg acaaaagcga ataatagtag catcgccaac ttttgtgttt
     3901 ttcttaatat cactcatcgt actataaata atagttttcc cagaatttaa aataaaatca
     3961 gcgacttttt tttcagactt cgttaaaaat ggataatttt ctttaattaa atagatataa
     4021 ctcatacaaa ctcctctaca atttcaactt ttctccatta tactccaaaa taataaaaaa
     4081 aactcctaaa tactccattt ttatctttac ttttatgaaa gcggatgcta tattgttttt
     4141 ggagttatta tccaaaaata ataaaaaatt ggagcgttta ggataatgga taaaaagagg
     4201 tttttagatc ttatttcaaa aggtttgatt gtttcatgtc aagcattacc tggtgaacca
     4261 ttttacaaag aggaagaaag tttaatgcct tattttgctt tggctgctca aagggctgga
     4321 gcagttggta tacgtgcaaa ttctgttcga gatattattg aaattagaaa gtgtgtatca
     4381 ttaccaatga ttggaattat taaaagagat tatccaccag aatctccatt cattactgcc
     4441 actatgagag aagtagatga attagtagaa accggtgttg aggtaatcgc tcttgattgc
     4501 actcttagaa atcgacatga tggactatct ataaattctt ttattactaa aataaaagaa
     4561 aaatatcctc atcagttatt tatggcagat atttctacat ttgaagaagg caaaaatgca
     4621 tataaagcag gaatagattt tgtaggaact actttaagtg gatatactga ggaaagtaaa
     4681 aaacaagatg ggccagatat agatttgatt aaaaaattgg tagaagaagg gatttgtgta
     4741 attgctgagg gaaggattca tactccagaa caagcaaaag agataaaaaa aataggagtt
     4801 gcaggtattg ttgtgggtgg tgctattact cgaccacagg aaattgcatc aagatttgtt
     4861 aaagcattaa gttaggggga gaaataaaat gtttaaaaaa ttttcacaat taggacgcgc
     4921 tttcatgtta ccaattgcta tattaccagt agccggatta ctattgggac tgggaggagc
     4981 gttaacaaat gagagtgcta taaatgctta tccattttta gatcaaccgt ggttgcatac
     5041 aattttaagt attatgagtt atgctggaaa tgcagttttt gcaaacctag cactaatttt
     5101 tgcaattggt attgctgtag gacttgcaaa tggagataaa ggaacagctg gtttagctgg
     5161 tggagtagct tatctagtat atactgcaac tattagtggg tttcttgaac ttttttcagc
     5221 aaaagatgca acactagata caggtgtagt gggatcaatt gttgttggtg gtactgttgc
     5281 atttttacac aatcgttata gaaaaattga attaccacaa ttcttaggtt tttttggagg
     5341 atcaagattt attccgattg tttctagttt agctgcaatc attattggtt ctttctttta
     5401 tttaatttgg ccaccaattc aaaatggttt agtagttgct ggtgaacaaa ttgcccaaat
     5461 gggtagttta ggaacttttt tatatggttt tttattgcgt ttaacaggtg ctgttggact
     5521 acaccacacc atttaccctt tgttctggta tacatcttta ggtggtaccg aaactgtagc
     5581 tggatcaaca attgctggag cccaaaatat tttctttgcc caactagctg atccaaacca
     5641 cactggtctg tttacttatg gtactagatt ttttgcagga agatttgcaa caatgatgtt
     5701 tggattacca gcagcatgtt atgcaatgta tcgtgcaatt cctaagaaaa atcgtaaaaa
     5761 gaatggtggg ctttatttta gcggggccct tacatcattt ttaactggaa ttactgaacc
     5821 agttgaatat atgtttttat ttgttgctcc ttggctttat gtgattcatg cattcttaga
     5881 tggattatct ttttattttg ctgatatctt aaatattcgt gtagggaatt ccttttcagg
     5941 tggtttgatc gactttttat tattcggtgt acttcaaggg aatgacaaaa ctaattggtt
     6001 aaaattaatt ccttttggta ttgcatgggc atttatatat ttctttgttt ttacattctt
     6061 tatcaaaaaa tttaaggtag ctattcctgg aatggaagaa gaatcagaag ttcattctga
     6121 aaaagtaata aacaaaaata catcattaca tgaagagtct cttatcatta ttgaagctct
     6181 tggtggaaaa gaaaatattg agtctgttga agcttgtgca actcgtttgc gtgttgctgt
     6241 tacgaatggg gagattattc aaaaacaaac aataaaagac actggtgcaa ctgctgtctt
     6301 cgaagtgaag ggtggcatcc aagctgtttt tggagggaaa gctgacttat taagtcaaga
     6361 aatcaatcaa atattaggaa aagataatta ggaggttttt aatatgtttg aaattttcaa
     6421 aaagaaaaaa caagaaactt tttatgcacc ttgttccgga aaaatcattt ccatcacaga
     6481 agtaaatgat cctgtctttt cacaaaaatt actaggtgat ggttttgccg tcatacctga
     6541 tggttcaaaa atttatgctc cattggctgg tcgagtaaca aatatatttc ctactaaaca
     6601 tgctattaat ataaaaagca actcaggcat tgattatttg attcatattg gaattgatac
     6661 agttgaatta caaggagagc ctttttcgat ttgtgttcaa gaaaatgatg aggttactcc
     6721 tgaaacctcg ttggttgatg tggattttga caaaataata aaatctggta aagatacttc
     6781 tgttatagtt gtttttccgg agaaaaatca gttcaatgat ttaataatag ataatggtcg
     6841 aattgctcat gggaattcat gtggaaaaat cactttttaa aaaagtgtat ataaaaagct
     6901 ataaaataag ctaaatagga tatttgtcaa atgaagttgg tgaacagaaa gaactgtttt
     6961 ctttctgttc atttttttat tttattagta atgattcccc atgccaaatc atgatccgta
     7021 atttaaagtt ataaaagctt cgaaatccat tggcatttcg ttggaatacc ttgatatgat
     7081 tattcaaata taaaatatag agagaagaga gagagggaag atatttataa agggcaaagt
     7141 tatgtttact caacttagga aatagaaata gatcaattta tattctcttg tcaatcgaat
     7201 atgaacctta gcactatata ccaaatatag taaaatgctg aaagatgt
//
LOCUS       DASJUF010000122         7238 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJUF010000122 DASJUF010000000
VERSION     DASJUF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.124"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            122..769
                     /locus_tag="U4B62_002460"
     CDS             122..769
                     /locus_tag="U4B62_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2142586.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    898..1114
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1162..3807
                     /locus_tag="U4B62_002461"
     CDS             1162..3807
                     /locus_tag="U4B62_002461"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN2142587.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4117..5430
                     /locus_tag="U4B62_002462"
     CDS             4117..5430
                     /locus_tag="U4B62_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN2142588.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5420..6073
                     /locus_tag="U4B62_002463"
     CDS             5420..6073
                     /locus_tag="U4B62_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2142589.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6128..6820
                     /gene="radC"
                     /locus_tag="U4B62_002464"
     CDS             6128..6820
                     /gene="radC"
                     /locus_tag="U4B62_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN2142590.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6970..7170
                     /locus_tag="U4B62_002465"
     CDS             6970..7170
                     /locus_tag="U4B62_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2142591.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2391 a         1225 c         1589 g         2033 t
ORIGIN      
        1 tgcaagcttt tgaacaccgt tttatttgtc tcacaccctt ttccttccta cttgtaacct
       61 tgctcccgtg ttagaaacat gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt
      121 tatgcgagaa aaaacagata cactgccaaa agcaactgcg aaaagattac ctttatattt
      181 acgctacttg aagatgctgg atgactcagg tataagtaga atcaaatcaa atgagttcag
      241 cgaaatcacc caaattcctt ctgcaacgat ccgcagagac ttctcgcaat taggcgagct
      301 aggcagaagc ggatatggct atgatgtccc ttttcttatc gatgtattca ataatatcct
      361 taatactaaa gaagaaaaac gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt
      421 gaaacacaac aattttagaa gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga
      481 tccagcactg atcaatcgag taattgatgg agaaatgatc tatccgattg atcgtttcgc
      541 ggagatagcc aaagcaaaaa atgtgacagt agcaatctct acggtaccaa gtaaatattc
      601 acaatctgct attgatgaaa ttgtcaaagg aaatgtaacc gctatactta attttgcgcc
      661 tgacagagta actgtcccag cttatgtcaa tgttcaatac attgatttga caaccgagtt
      721 gcagacgctt atttactttg atgagaatta ctcagaagtt ttttcttagg aaaatttgag
      781 taggcttatt gatcttgcga gaactcgtcg taattctttc aaaaattttt tagtactaaa
      841 tgaagtggtt ttttcacgac cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc
      901 ataacgctga acaagaggag taactggcac attcattctt agagagagaa ctatctgctg
      961 aaaggttctc aatgacaacc agtgaaggta gcttgggagt ttctgtcttg aatggagtag
     1021 agatagacga gtcgtgatcg ttaactcact tgagaggcaa taatgattga ttattgcaat
     1081 ttaggtggta ccgcgtaaat catacgtcct agaatacgaa agtattttag gacgtttttt
     1141 agtttaaagg aggaaaacag catgacggaa gaaaataacc tatcaacaaa atataatccg
     1201 caagaagttg aagctggacg ttatcagcat tggttagaac aagatttgtt taaaccgagc
     1261 ggagataaaa aagcaaaacc ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa
     1321 cttcatttag ggcatgcttg ggatacgacc ttgcaagata tgctgattcg tcaaaagaga
     1381 atgcaaggat atgatacttt atggctgcct ggtatggacc atgcaggaat cgcgacacaa
     1441 gcaaaagtag aagcgaagct tagagagcaa gggatttctc gttatgatct aggtcgtgaa
     1501 aagttcatcg atcaagtgtg ggaatggaaa gaagagtatg cgagccacat ccgcgaacag
     1561 tgggctaaaa tgggcttatc tttagattac agtagggaac gttttacatt agatgatgga
     1621 ttatctgaag cagtacgtaa agtttttgtt accttatacg aaaaaggttt gatttatcgt
     1681 ggtgaatata tcatcaactg ggatcctcaa gcacgaacag ctctatctga tatcgaagtt
     1741 attcataaag atatcgaagg cgccttttat catatgagct atgaactagc agatggatca
     1801 ggcgtagtag aaattgcgac aacccgtcca gaaacgatgt tgggagatac ggcgattgct
     1861 gttcatccag aagacgagcg ttatcaagcg ctcatcggca aaaaagtgat tctaccttta
     1921 gtcaataaac aaatcccaat cattgcagat gaatatgtag atatggaatt cgggacaggt
     1981 gtagtaaaaa tcactccagc acatgatcca aacgactttg aggtaggtaa tcgtcatgat
     2041 cttccacgcg taaatgttat ggatgatgat ggtacgatga atgaactagc aggaaaatat
     2101 gctggaatgg aacgttttgc tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt
     2161 cgattgatta aaatcgatac gatgatccac agtgtgggtc attccgagcg gacaggtgtc
     2221 gttgttgaac ctcggctgtc tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa
     2281 gccattgata accaagaaac agatcaagca gtggaattct tccctccaag attcaatcaa
     2341 acgtttttac gttggatgga aaatgtccat gattgggtaa tctcacgaca attatggtgg
     2401 ggacatcaaa ttcctgcatg gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa
     2461 gcaccagcgg atagtgaaaa ctgggtacaa gatcctgatg ttttagacac ttggtttagt
     2521 tcagctttgt ggcctttctc aacaatgggc tggccagata cggaaagcga agattataag
     2581 cgatacttcc ctactagcac tttagtaacc ggttatgaca tcattttctt ctgggtaagc
     2641 agaatgattt tccaaagctt ggaatttact ggcgaacgtc catttgagaa tgtcttgatt
     2701 catggattga ttcgtgacga acaagggcgg aagatgagta aatcattagg taatggaatc
     2761 gatccgatgg aagtaatcga aaagtatgga gcagatgctc tccgctggtt cttatcgaat
     2821 ggttctgcac caggacaaga tgtccgcttc agctatgaaa aaatggatgc ttcttggaac
     2881 tttatcaaca aaatctggaa tgcttcacgt ttcgttatca tgaatatcga aggaatgacc
     2941 gtagaagaga tcgatttatc tggagaaaaa tcagtggctg accggtggat ccttactcgt
     3001 ttgaatgaaa cgatcgaacg agtaactgag ctatttgatc gttttgaatt tggtgaagca
     3061 ggtcgacaac tgtataactt tatctgggat gatttttgtg actggtacat cgaaatgagt
     3121 aaagaagtac tttatggaga tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc
     3181 catacacttg atcagatcct acgtttattg catccaatca tgccattcgt tacagaagaa
     3241 atatgggaac atattcctca tcagggaaat tctctagtag tggcagaata tcctgttgta
     3301 cgtccagagt ttgatgatga aacagcttct aaagggatgg aagtattgaa agaattgatt
     3361 cgagccgttc gaaatattcg ttcagaagtg aatacaccat tgtctaaacc aattacgttg
     3421 ctaattaaaa caaacgatgc taaaatcgat caatttttag ttgaaaatac aagctatatc
     3481 gaacgcttct gtaatcccga agagttgacg atatcaagtg agatcactgc gccagatcta
     3541 gcgatgtcag ctgttctgac aggtgcagaa atcttcttgc cgttagcagg attgatcaat
     3601 attgaagaag aaatcaaacg attagaaaaa gaactggaaa agtggacaag cgaagtaaaa
     3661 cgtgtccaag gaaaactttc taatgaacgt tttgtttcta acgcgccgga agaagtagta
     3721 gaggcagagc gtgcaaaaga aaaagattat ttagagaagc aaacggctgt caaagaacga
     3781 atcgaaagtt tgcgtactat tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct
     3841 attactaaaa gctagctgat tgaacatcgt tacaggaaag agtttgagcc tcactcttag
     3901 cttcgattca taaggagatg tgacataaat tttgtcgcat ctctttttta gtctactctt
     3961 attatgtagg agaaggaggg aggtggatca tgtatcaaga gtcaataaag tagttgataa
     4021 aatcgttcct ctctctgttt cttttctctt tgtcatactt ttatttttct tatgtgttct
     4081 atttgttata ataaaagaga aatgggggaa ttgttgatga cgattgaaga agcaatagaa
     4141 tggatccata gtcgattacc atttggttca cgtcctggcc ttgaccgagt cgaagcttta
     4201 ttagaagtgg tagggcatcc agagagaaaa gtaaaaacga tccatatcgc tggaacaaac
     4261 ggaaaaggtt ctactgtagc ttatttgaga agcctattag aagaggctgg gctgacagta
     4321 ggtacattta cttcacctta tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa
     4381 agcattccag acaatgattt gatccagctt gtaaaaaaat atcagccact gatcaaacaa
     4441 cttgatcaat ttgaagcagt agctggtatt actgaattcg agacattaac agcaatggca
     4501 tttgattatt ttttgatgaa gaaagtggac gtagcgatta tcgaagttgg cttaggcggc
     4561 ttattggatt ctaccaatgt agctgtacca atgctgacgg gaataacaac gattggactt
     4621 gatcataccg aaatacttgg agatacaatt gaagaaatcg ctgcacaaaa agctggaatc
     4681 atcaaacaag gcgttccagt agtaacagga aatattgccc cagaagcttt tactgtcatc
     4741 caacaaacag ctaaagcaaa aaatgcaccg ctttatgtat acggggaaaa ttataaaata
     4801 aactatctgc atcctgatga gatgtgggga gaagtatttg aattttttaa tgaaatagga
     4861 aagatccctc aattaaccac tccgctttta gggcaacatc aagctgaaaa tgcaggagta
     4921 gccatcgaat tgtattttgt ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat
     4981 gttttgaagg gattgaagaa atctcgatgg ccaggaagaa tggagcgttt gagtaacgag
     5041 ccattagttg ttttagatgg tgcccataat acgcatgcaa tgaaccggct tgttgagaat
     5101 ttgaaaaaag aattcaaagg atatcaaatc aacattttgt tttccgcctt acgaactaaa
     5161 aatgttagag aaatgctaga acgattgcag caagtaccaa atgtccatat ttatttgacg
     5221 acctttgaac atccaaaagc catcgaatta agtgaagtat cagatttagc aagtgagaga
     5281 gtatcaatcg tttcattgtg gcaattcgga ttagcagata tattggaaaa gatgacaaat
     5341 gaagacgtat tagttgtgac cggttcactt tatttcattt cagaagttcg ccatttatta
     5401 atacaattag gaggagatca tgaagtataa tgcagtgatt tttgacatgg atggtttgct
     5461 gttcgacaca gagttagtgt attacgaagc cacacaagta gtagcagaca agatgggttt
     5521 tccttatgat aaagacctgt atttaaaatt cttaggtgtt tcagatgaag aagtatgggc
     5581 gaattatcat atgattttct cggaatttgg caaaacaagt gtacaaagat tcatagatga
     5641 ttcataccag gaaacgcttg aacgtttttc gcaaggagcg gtacaaatga agcccggtgt
     5701 gttggaactc ctctcttttt tagaagaaca tcagattccc aaagttgtcg cttccagtaa
     5761 ccaacgagca gtcattgaac ttttattgaa gaagaacaat ctgtttgaac gatttgagac
     5821 gattgtttct gcagaaaatg ttcaacgagc aaaaccagac ccagaaattt tcttgttagc
     5881 taatcagaag ctaggtacag cgaagaaaga aacattgatt ttagaagatt cacaaaatgg
     5941 tattttagca gcttctgcgg ctgagatccc ggtagtcatg gtaccagatc tgcttccacc
     6001 atcccaagaa ttgatcaaaa aaacagctgg ggttgtttct tctttacatg aaatccctgc
     6061 attgttaaaa taaaaatcca tctcttttcg tatcttattt tagataagat agaaaggaga
     6121 tgtttttttg ggaaaacgat tgatcaaaga agtacctact agctctttac caagggaacg
     6181 tatggaaatt tatggcgcgg aagctttatc ggatcaggag cttttggcaa tcttactgcg
     6241 taccggtcaa cacccttata gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg
     6301 aggactggcc tctttacgac aggctacttt gcatgagtta gaagagattc gagggatagg
     6361 acgcgtgaaa gcaatcgaaa tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga
     6421 tcatgaaaga gcggctccac cggttcgtac cagctacgaa ttggcccagc aattgattct
     6481 ggaaatgaaa gatcacaaac aagaacattt ggtttgtatt tatttggaca cgaagaatca
     6541 agtgatctta aaaaaaacag tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag
     6601 agaaattttc cattatgctg tacgttattg tgcagcaagg atcgttttag cgcataatca
     6661 tccttctggt aatgttattc catcgcagca ggatatgaat tttaccaaaa ggatacaaaa
     6721 gtgtggagaa atgatgggga tcactgtact ggaccacttg atcatcggac gcaaacgata
     6781 ttttagtttg agagaagaag gaatgatgga agagaagtaa gttgctttct tgcaaaaaaa
     6841 atgaagcaag tgtacaatta tattgtaatt ttgtttggga atagaagttg aaacatctgt
     6901 tgcacctcta tcgtacacga gcaatgttac aatttaaatt agtgcttttg gcacgaggag
     6961 gatattatta tggaaaacgg aacagtaaaa tggttcaacg ctgaaaaagg atttggattt
     7021 atctcacgtg aagatggaag cgacgtattc gttcacttct cagctatcca aggtgacgga
     7081 ttcaaaacat tagaagaagg tcaagcagtg actttcgacg ttgaagaatc agaccgcggc
     7141 ccacaagctg caaacgttgt aaaagcttaa ttttatctgt aaatcaaatt gatggtagac
     7201 atcaattttt acgagagaac aaagcgagta acaaaaat
//
LOCUS       DASJUF010000123         7217 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.125, whole genome shotgun sequence.
ACCESSION   DASJUF010000123 DASJUF010000000
VERSION     DASJUF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.125"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="U4B62_002466"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="U4B62_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN2142592.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /locus_tag="U4B62_002467"
     CDS             complement(919..1677)
                     /locus_tag="U4B62_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2142593.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="U4B62_002468"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="U4B62_002468"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2142594.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="U4B62_002469"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="U4B62_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN2142595.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="U4B62_002470"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="U4B62_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN2142596.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="U4B62_002471"
     CDS             complement(4433..5290)
                     /locus_tag="U4B62_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN2142597.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="U4B62_002472"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="U4B62_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2142598.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DASJUF010000124         7131 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.126, whole genome shotgun sequence.
ACCESSION   DASJUF010000124 DASJUF010000000
VERSION     DASJUF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.126"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            255..521
                     /locus_tag="U4B62_002473"
     CDS             255..521
                     /locus_tag="U4B62_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN2142599.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            521..2248
                     /gene="ptsP"
                     /locus_tag="U4B62_002474"
     CDS             521..2248
                     /gene="ptsP"
                     /locus_tag="U4B62_002474"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN2142600.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            2412..3458
                     /locus_tag="U4B62_002475"
     CDS             2412..3458
                     /locus_tag="U4B62_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2142601.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            3475..4698
                     /locus_tag="U4B62_002476"
     CDS             3475..4698
                     /locus_tag="U4B62_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2142602.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            4787..5674
                     /locus_tag="U4B62_002477"
     CDS             4787..5674
                     /locus_tag="U4B62_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN2142603.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            5760..5990
                     /locus_tag="U4B62_002478"
     CDS             5760..5990
                     /locus_tag="U4B62_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN2142604.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(6047..7012)
                     /locus_tag="U4B62_002479"
     CDS             complement(6047..7012)
                     /locus_tag="U4B62_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2142605.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         2442 a         1187 c         1492 g         2010 t
ORIGIN      
        1 aaacgtcagg agaagagaaa gcgacaagaa aaaggaaata aacacgtttg ttataaaatg
       61 tgaagaaaag gatgaaaaag aatccggaat aatttgaata cttttatctg aaattgaatg
      121 aaaactgttg tatctaacga aaaaaaatgt taatctttac agatgaaagg gtggcatttg
      181 ttaggattct ctgaattact aaaaaagaaa accctcacat aaaaacaaca ctcacttaaa
      241 ggagaccgat taatatggaa aagaaagaat ttcacgtagt agcagaaact gggatccacg
      301 ctcgtccagc tacactatta gtacaaacag caagcaaatt taactctgat gtaaacttag
      361 agtataaagg taaatcagta aacttgaaat caatcatggg cgttatgtct ttaggtgtag
      421 gtcaaggttc tgatgttact attactgcag aaggtgctga cgaagctgat gcaatggcag
      481 caatcgttga aacaatgaag aaagaaggct tatctgaata atggttgaaa tgctaaaagg
      541 gattgccgct agtgacggtg tagccgttgc aaaagcttac ctgctagttc aaccggattt
      601 aacattcagt aaggcaacag tagaagatac tgcggctgaa gaagctcgtt tggatgctgc
      661 attagctaaa tctactgaag aattgcagca aattcgtgaa aaagcagcgc aaagcttagg
      721 tgaagcagaa gcgcaagtat ttgatgccca tttgatggtt ctttcagatc cagaaatggt
      781 aggtcaaatc aagcaaaata ttaaagacaa cagcgtaaat gctgaatcag cgctaaaaga
      841 agtaacggat atgtatatcg gtatgttcga agcaatggaa gataatgctt atatgcaaga
      901 gcgtgctgca gatattcgtg acgttgctaa acgtatttta gcgcacttat taggcgttac
      961 tttaccaaat ccatcaatga tcaacgaaga agtggtcgtt gttgcacatg acttgactcc
     1021 aagtgataca gcacaattag atcgcaattt tgtaaaagcg tttgtaacag acattggtgg
     1081 acgtacttca cattctgcta tcatggcacg ttctttagaa attccagcaa tcgttgggac
     1141 aaaagaaatt actgctaaag tcaaagaagg cgatattttg gctgtcaacg ggatcgaagg
     1201 cgatgtaatc atcgatccta cagatgaaca aaaagctgaa tttgaaaaag caggtgcaga
     1261 ttacgcagca caaaaagcag aatgggaaaa attaaaaaat gcagaaactg tcacagctga
     1321 cggcaagcat tttgaattag ctgcaaacat cggaacacca aaagatctag ttggtgtcca
     1381 taataatggt ggagaagcag ttggtctgta ccgtactgaa ttcctgtata tggattctcc
     1441 agacttccca acagaagatg accaatatga agcttacaaa gctgtattag aaggtatgga
     1501 aggaaaacct gtagttgttc gtacaatgga catcggtgga gataaagaac taccatatct
     1561 gcaattacct catgaaatga atccattctt aggatatcgt gcattgcgta tcagtttatc
     1621 tgaacaaggc gacgaaatgt tccgtacaca aatgcgtgca ttgcttcgtg catctgttca
     1681 tggtaattta cgtatcatgt tccctatggt agctacactc aaagaattcc gcgcagcaaa
     1741 agcgatcttt gaagaagaaa aacaaaaatt gataagtgaa ggcaaggaag tatctgatac
     1801 gatccaagtc ggcatcatga tcgagattcc agcagcagca gtcttagcag acaagtttgc
     1861 aaaagaagta gatttcttct ctgtaggtac gaacgatttg attcaataca caatggcagc
     1921 agaccgtatg aacgaacgcg tttcttactt gtaccaacca tataacccat caattttacg
     1981 tttgatcaaa aatgtaattg atgcagcaca cgctgaagga aaatgggcag gtatgtgtgg
     2041 agaaatggct ggagatcaaa cagctgttcc attgctagta ggtatgggtc tagatgagtt
     2101 ctcaatgagt gcgacatcta ttcttaaaac gcgtagttta atgaaacgct tggacacagc
     2161 gaaaatggct gaacttgctg atcgtgcatt gaaagaatgc gatacgatgg aagaagtcgt
     2221 tgaacttgtt catgaatatg taaaataaac aacgtatcaa aagctggtgt ggcttgtaaa
     2281 agagctacgc cagttttttt gttctttttt aagaaaacgt ttttgtcagt aagaatagac
     2341 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
     2401 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
     2461 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
     2521 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
     2581 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
     2641 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
     2701 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
     2761 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
     2821 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
     2881 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
     2941 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
     3001 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
     3061 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
     3121 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
     3181 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
     3241 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
     3301 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     3361 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     3421 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     3481 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     3541 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     3601 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     3661 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     3721 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     3781 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     3841 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     3901 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     3961 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     4021 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     4081 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     4141 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     4201 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     4261 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     4321 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     4381 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     4441 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     4501 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     4561 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     4621 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     4681 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     4741 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     4801 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     4861 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     4921 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     4981 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     5041 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     5101 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     5161 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     5221 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     5281 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     5341 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     5401 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     5461 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     5521 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     5581 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     5641 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     5701 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     5761 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     5821 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     5881 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     5941 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     6001 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     6061 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     6121 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     6181 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     6241 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     6301 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     6361 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     6421 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     6481 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     6541 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     6601 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     6661 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     6721 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     6781 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     6841 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     6901 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     6961 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     7021 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     7081 aattctatag ttctgttcac agaaaatact ttgataatca aaatatgtga g
//
LOCUS       DASJUF010000125         6856 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJUF010000125 DASJUF010000000
VERSION     DASJUF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.127"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..523
                     /locus_tag="U4B62_002480"
     CDS             <1..523
                     /locus_tag="U4B62_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEN2142606.1"
                     /translation="DLISEYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDL
                     KKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKV
                     TDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQR
                     GIKNLVKSYLKMN"
     gene            526..1422
                     /locus_tag="U4B62_002481"
     CDS             526..1422
                     /locus_tag="U4B62_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2142607.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1472..1861
                     /locus_tag="U4B62_002482"
     CDS             1472..1861
                     /locus_tag="U4B62_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2142608.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1848..3350
                     /locus_tag="U4B62_002483"
                     /pseudo
     CDS             1848..3350
                     /locus_tag="U4B62_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4095..5921
                     /gene="ltrA"
                     /locus_tag="U4B62_002484"
     CDS             4095..5921
                     /gene="ltrA"
                     /locus_tag="U4B62_002484"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2142609.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            6055..>6856
                     /locus_tag="U4B62_002485"
     CDS             6055..>6856
                     /locus_tag="U4B62_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2142610.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2389 a         1114 c         1470 g         1883 t
ORIGIN      
        1 tgatttgatt tcagagtata aaaatgagat tgaaacatgt gtaaacgaaa taagaaaatt
       61 aaattgggat attgtttctg tgaaaaaaga aatacaagat aaggcgaagg tagagcttga
      121 aattgaagat ttaaaaaaac aagtagaaaa gctatctcat aagagttata aagatgttta
      181 tgatgagtat tcaaaagaga cagatattta tagggaactg aaacgtgatg tggaggcact
      241 taaggaaatt cccaaaaatc taactgatag tgttgacaaa attgactttg gcaatggtat
      301 aaaagttaca gacgaaattg atgaacaccg cggagaattg attaagaatt tagtgaccaa
      361 tcgggctaag attcaaacaa taattaatga aatgtcagat gaaattgata gttatagagc
      421 agagctgaat aaatcagatt ggaaagttaa ctataatgag gtttcaaaga ggtatcaaag
      481 aggtatcaaa aacttagtga agagttatct caagatgaat taaaaatgtt acaaaacctt
      541 gattctttat atcaatcttt atcaaaaact gaagagcgat tggaagaaat tattcaacaa
      601 gaaataaagc ttaaagatta ccaagttgag gttgactcag caatgtcaag aatgaataaa
      661 tcatatcaaa atttctattc tgagagaagt agttcctcgc gcgaaatttt ttctgatatt
      721 acaggaatag aggctactat tcaaccaaat agggatttca ataattatgt taaagagctc
      781 agagcttgta ttaataagga ggacactttt gacagtgagt ttggatctct tattgataag
      841 ctgacaaaaa attccattac taatcaagat atatataatg acatacttga tataagagtt
      901 agtggggaaa gtagtatttt ttcagataaa aaattaatca attctataag aaaattgaat
      961 gatgcacaag ttcttgaatt acaaaccctt aagccaaatg atttagtagt tatccgactt
     1021 gaactaaatg gaaaaaaagt tagtttatcg aatgctagtg ctggtcaaaa gacatctgca
     1081 attctttcta tgattttggc gtatggaaat tcaccactca tattggatca gcctgaagat
     1141 gatttggata gtcaattaat taataactta atcgtaagaa gtatcattac aaaaaaggaa
     1201 aacagacaaa ttttaattgt aactcataat gcaaatatcc cagttaatgg tgattcagaa
     1261 tggttgatta gcatgggaga ttcaaaagaa atatcggctg atcagtgcgg gtcggtagat
     1321 gaacccaata tcaagccaag aatatgtact gtgatggaag gtggcgagga tgcttttaca
     1381 aatagagcga aacgctatgg ttttaaacaa attgtagaat aacactagag ggtgagacat
     1441 attattatgt ccgccctttt taggaggaaa aatgaaaaaa ataaaaagct ataccagtat
     1501 ctgggcagtt gaaaaagtca tttacgcaat caatgatttt cagttgcctt ttcctgtgac
     1561 ttttaaccaa atggcttggt tcgttttatc tttactgttt gtcatcgtat ttgcccatgt
     1621 gcctccacta tctatgatag aggaggtctt tttaaaatac ttaggcatac cggtggctgt
     1681 cacttggttt atgtcgcaaa agacgtttga tggcaagaaa ccactgggat ttttacgatc
     1741 ctttatcagc tatcacttgc gatttaaggt gacttttgca ggaaagaaag tcaaagaaca
     1801 gaagaaacga tgggatgaac ccagtactct tgtaaggagt gtgaactatg tatccgatta
     1861 agtatattga aaataatctc gtctttaacc aagaaggcga gtgttttgcc tactatgaat
     1921 tagtgcctta caattactcc tttttgtcac ccgaacagaa atttcaagtg catgataact
     1981 ttcgccaact aattgcgcaa aatcgtgaag gcaagatcca tgcgttacaa attgccacag
     2041 aaagtagtat tcgcgccaca caagaacgat caaaaaaaga aattaccggt agactcaaag
     2101 aagtagcgaa acaacgaatt gatatacaga cagatgcatt agtatccatg attggcgata
     2161 gtcagattga ttatcgcttt tttattggct ttaaactaat tgcgacagat gaagaagtca
     2221 atctaaagag tctcaagaaa tctttctttt ctgggcttca agaatttgtc tatggagtga
     2281 atcatcattt gatgggcgat tttgtttcct tatccaatga agaaattcgc tgctattcta
     2341 agcttgaaaa attaatggaa agcaaattgg ctcgaagatt taaagtcagg cgagtcacac
     2401 cgagtgacct gacgtatttg attgaacata tttatggcga aaaagggata ccgtttgaag
     2461 aatacgaatt tcagctacca aagaaaaagc taaaatcaga aacactggtg aaacgttatg
     2521 acttattacg tccaaatcgt tgtttaattg aagaaaaacc aaggtgctta cggatggaac
     2581 atgagaatca tgagtcgtac gtagcgtatt taacaattaa tacaattgtg ggggagatgg
     2641 aatttccttc ctcagaactt ttctattacc agcaacaaca atttacattc ccaatcgaca
     2701 caagtatgaa tgtagagatt gtgacgaata aaaaggcttt agctaccgtt cgtaataaga
     2761 aaaaagaact gaaagattta gacaatcatg cttaccaatc tgacaatgaa acgaattcca
     2821 atgtcttaga tgctttggat tccgtggatg aattggaaac caccttggat cagtcaaaag
     2881 aatctatgta caaactgagt tatgtggttc gagtgagtgc cgaatcagtg gatgaactaa
     2941 agcgacgctg tgatgaagtg ctcgattttt atgatgatac caatgtaaaa ctagtccggc
     3001 cctttggcga tatgatgggg ctacatgaag aattcttacc cttaagtaaa cgatatatga
     3061 acgactatat tcagtatgtt acgtctgatt ttttggcggg gcttggtttt ggggctactc
     3121 agatgctagg agaattagaa gggatttact ttggctacaa tgtggataca ggtcgtaatg
     3181 tttacctgaa accggcattg gcttcacaag gagtcaaggg ttcagtaacc aatgcgctgg
     3241 ccgccgcttt cctgggttct cttggtggag gtaaatcctt tagtaacaat ctcttagtct
     3301 actatgctgt tttgttcggt ggtcaagctg tgattgtcga tccaaaaggg ggtgcgaaac
     3361 gttcctactt gaaaagagta ggcacagcac ttcattaaat caatatttaa tgtgtgttgg
     3421 agaactaaac acacgaatag tcgaatgacg gggtaacgcc ctgaaaggct actttaatac
     3481 tctgactggc agaaacttga gtaaacaagg ctctgtcaga agctcggtga agtcggcaga
     3541 cagacaccgt aacagataat gctgacgaaa gattcccaga ggtgggcgtt gtgtcctata
     3601 tgccggaagt ctataaaata tctatggtta gaatgtagat gagaggtcta ctgacgaatc
     3661 cttgaatgta caggtctata tcggggctgg atagaaatgt ccagtgacac aaacagtgtc
     3721 gctactgtgg gtgagtaaga ctccgcattt atgaaaatcc atataacgtt aaaggcgtta
     3781 tcaaggtagc aggcttatag aggaaaccta aggatatatg tacagatagt gtgtttggaa
     3841 cgttgaaagc tgacaacatg gagattttac tgtctgtgaa gtgttcacat cagaaagtta
     3901 ttgtgagatt agcaatgata taaggtgtgt taatgtcagt gagagtggtg gcacagtacc
     3961 tatgaaaccg tgataacaag cggtggaggg atagccacta gtcatatgaa gaatagaatt
     4021 actaagaaaa atggtatggg ttcgagtaag actaagagat gtacttgctc ctgcaaacaa
     4081 ggagggtaca gaccatgcta aagaaaaaca agctccgcta taacgagtat tatgatatgc
     4141 aacatattta tgacgagtta tacgcacaga gcaagaacgg taataacttt tataagttac
     4201 ttgaaattat tggttcggaa caaaatattt gtttagctta ccgtaatctg aaatcaaaca
     4261 gtggcagtaa aacggctgga acggatggaa tgacgataga cgatataaaa caactcagca
     4321 atgctgagat tgttgctacc gttcgggaaa gccttagcaa ctaccgcccc aaatcagtca
     4381 gacgtgtttt cattccaaaa gctgggagcg ataaaatgcg tccattgggg attccgtgta
     4441 tctgggacag gctggtacaa caatgtatcc tgcaagtcct agagccgata tgcgaaccaa
     4501 agttccataa tcattcctat ggattccgtg cgaatcgaag tgcccaccat gcagtcagca
     4561 gagtaacgac actgataaat ttatcaaaat atcattactg tgttgacgta gacattaaag
     4621 gatttttcga taatgtcaat catggaaaac tattaaaaca aatctggaca cttggtattc
     4681 gggataaaag attgatttgc attatcagca aaatgctgaa agccgaaatt gacggcgagg
     4741 gtgtcccaga aaaaggaacg ccacaaggcg gtctgttaag tccactgctc tccctcatag
     4801 tccttaatga attagactgg tgggtaagca gtcagtggga aacattccag ccgaagaacc
     4861 gcagtaaaaa tgggtggctt caatacgcaa agaaatatac caaactgaaa agcggtttta
     4921 ttgtacgtta cgctgatgat tttaaaatca tgtgttcaac ctatggagaa gcacaaagat
     4981 tttaccacag taccgtagat tttcttaata aaaggctgaa attagaaatc agtccagaga
     5041 aatcaaaggt agtaaatctg aagaaaaatt catcagattt tctggggttc aaaatcaaag
     5101 tcattccaaa aggaagaacg aaacacggtt atgttgccaa aacagatatg aaccaaaaag
     5161 cattaaagaa agcgaaaaca aatctaaagt taaaggtaaa agatatagcc agacatacga
     5221 ctggcttcaa tatcagcaga tataatctga cagttatcgg tatgcagaat tactactgca
     5281 tagcaactaa cgtatataac aacctgacag aagtaagtta cgctctctta ccgaccatac
     5341 gaatccgttt aaggaacatt gcaaaatcag ttccatttga aagtacaagt tcagaatttc
     5401 agagcaggac aaaaggaatc agaccaaaaa caaaaatagt gatgattgcg gataatccgc
     5461 tattgccgat tcaaggagta caacacaaaa atcctatgaa tttctcgcaa gatatttgta
     5521 attttacaaa gcaagggaga aacaaggtgc atgaggacgt tgtagttgta acaaaagaag
     5581 aaatccgagc gttgttggaa aatgaaaacc ctgccgatag cgtagagttt aacgacaatc
     5641 gtatttcggc atatatcgca caacaaggca actgctatgt aatgaatcgt cgaggaactc
     5701 cgtccactct gatatgtatt cacaaatcag acaaaggaga taaccttgac agatattcta
     5761 atctggcatt tgtggaaatt cccattgcaa aggcaatcct aacagaatca gcagacgaag
     5821 caaaatcact gttaaaaggt tatgttctta atagtcagca gaagaaaaaa ctgaacagaa
     5881 tacggacgaa ttacggatac cagacaataa ctgggaaata acttataaat ctatttggat
     5941 atgatggaac gccgtatgaa gcgaaagttt catgtacggt gtgaagcggg ggaaaacttg
     6001 gagataacat caaaaagtta cctatcgcta tagcgaggag ggtggaaaga aaccttgcca
     6061 gaaattgcgg atgaaatcag tatcctcaac ttgacgagta agtcagaaaa tcaaggtctg
     6121 cttgatcctt atgtgattat gaagaaaaag aaagactcgg aaagtttagc aattgatatt
     6181 ctgacttttt taacaggaat ttcaagtcga gacggggaga agtttcctgt gttacgtaaa
     6241 gccattcgaa cggtgactca aagtgatcaa cgggggttat tacaagttat taacgaattg
     6301 cgaaaagacg ggtcaccaat cgcggaaaat atcgccgatc atatcgagtc ctttaccgat
     6361 tatgattttg ctcatttgct tttctcagac ggctcgatta cccaatcgat tagtttagat
     6421 aaacagctaa atatcattca agtggcggat ttggttttac cggatgcaga aacgactttt
     6481 gaagaatata ccaccatgga attgcttagt gtttcgatgc taatcgtcat ttccactttt
     6541 gccttggact ttatccatag taaccgagaa atcttcaaaa ttgtcgattt ggatgaggct
     6601 tggagttttc ttcaagtggc tcaaggaaaa acgttatcga ataagcttgt tcgtgctggt
     6661 cgggccatga atgctggggt ctatttcgtc actcaaaata gcgacgattt attagatgaa
     6721 cgtttaaaaa ataatatcgg gttgaagttt gcttttcgca gtaccgatat tcatgaaatc
     6781 aagaaaacct tggaattctt tggtttggat caggaggatc aaagcaatca aaaacgcctg
     6841 cgagatttgg aaaacg
//
LOCUS       DASJUF010000126         6736 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJUF010000126 DASJUF010000000
VERSION     DASJUF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.128"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..520)
                     /locus_tag="U4B62_002486"
     CDS             complement(62..520)
                     /locus_tag="U4B62_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2142611.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(533..1864)
                     /locus_tag="U4B62_002487"
     CDS             complement(533..1864)
                     /locus_tag="U4B62_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN2142612.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1875..3317)
                     /locus_tag="U4B62_002488"
     CDS             complement(1875..3317)
                     /locus_tag="U4B62_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2142613.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3304..4068)
                     /locus_tag="U4B62_002489"
     CDS             complement(3304..4068)
                     /locus_tag="U4B62_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN2142614.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4172..5047)
                     /locus_tag="U4B62_002490"
     CDS             complement(4172..5047)
                     /locus_tag="U4B62_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2142615.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5302..5386)
                     /locus_tag="U4B62_002491"
     tRNA            complement(5302..5386)
                     /locus_tag="U4B62_002491"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5350..5352),aa:Leu,seq:caa)
     gene            complement(5404..5475)
                     /locus_tag="U4B62_002492"
     tRNA            complement(5404..5475)
                     /locus_tag="U4B62_002492"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5441..5443),aa:Gln,seq:ttg)
     gene            complement(5485..5557)
                     /locus_tag="U4B62_002493"
     tRNA            complement(5485..5557)
                     /locus_tag="U4B62_002493"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5522..5524),aa:His,seq:gtg)
     gene            complement(5619..5703)
                     /locus_tag="U4B62_002494"
     tRNA            complement(5619..5703)
                     /locus_tag="U4B62_002494"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5667..5669),aa:Leu,seq:caa)
     gene            complement(5724..5794)
                     /locus_tag="U4B62_002495"
     tRNA            complement(5724..5794)
                     /locus_tag="U4B62_002495"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5760..5762),aa:Cys,seq:gca)
     gene            complement(5840..5911)
                     /locus_tag="U4B62_002496"
     tRNA            complement(5840..5911)
                     /locus_tag="U4B62_002496"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5877..5879),aa:Gln,seq:ttg)
     gene            complement(5923..5995)
                     /locus_tag="U4B62_002497"
     tRNA            complement(5923..5995)
                     /locus_tag="U4B62_002497"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5960..5962),aa:His,seq:gtg)
     gene            complement(5998..6071)
                     /locus_tag="U4B62_002498"
     tRNA            complement(5998..6071)
                     /locus_tag="U4B62_002498"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6037..6039),aa:Trp,seq:cca)
     gene            complement(6078..6158)
                     /locus_tag="U4B62_002499"
     tRNA            complement(6078..6158)
                     /locus_tag="U4B62_002499"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6122..6124),aa:Tyr,seq:gta)
     gene            complement(6160..6235)
                     /locus_tag="U4B62_002500"
     tRNA            complement(6160..6235)
                     /locus_tag="U4B62_002500"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6200..6202),aa:Phe,seq:gaa)
     gene            complement(6271..6343)
                     /locus_tag="U4B62_002501"
     tRNA            complement(6271..6343)
                     /locus_tag="U4B62_002501"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6308..6310),aa:Asp,seq:gtc)
     gene            complement(6353..6425)
                     /locus_tag="U4B62_002502"
     tRNA            complement(6353..6425)
                     /locus_tag="U4B62_002502"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6390..6392),aa:Val,seq:tac)
     gene            complement(6441..6512)
                     /locus_tag="U4B62_002503"
     tRNA            complement(6441..6512)
                     /locus_tag="U4B62_002503"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6477..6479),aa:Glu,seq:ttc)
     gene            complement(6527..6616)
                     /locus_tag="U4B62_002504"
     tRNA            complement(6527..6616)
                     /locus_tag="U4B62_002504"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6580..6582),aa:Ser,seq:gga)
     gene            complement(6656..6729)
                     /locus_tag="U4B62_002505"
     tRNA            complement(6656..6729)
                     /locus_tag="U4B62_002505"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6693..6695),aa:Asn,seq:gtt)
BASE COUNT         2027 a         1493 c         1098 g         2118 t
ORIGIN      
        1 tcaaaaaaat tttgtaaatt cacccataaa tatcacccta tttaactttt ttcatcgtac
       61 actaaaattc aaaacaacta tctttaatct ttacttcttg gttttgtttt aataaatttc
      121 cttcaaaaat gcctggagaa actacaatga tcaccgtatc tttttgtgct tgctccaact
      181 tcttcaaatc tataatagct aacagtgtcc cttcttttac ctcctggtta ctttcaaccc
      241 gtatctgaaa cggacttcct tcgagttcaa ctgtatctaa acccatatgg atcagtattt
      301 ttctgccaga ttcactttcg atcgttatgg catgtcgggt aggaaatata ttatcgatta
      361 ttcctttaac tggcgcatgg atatatccat catgattcgt tatagcgtat ccaactccta
      421 tcatttttga agaaaagacc tcgtctttta ctgctgttaa tggaataatt tgaccactag
      481 ctacagcttt tagaatgttt cttgttttaa agaaatgcac tttttatcac ctctacttta
      541 atgctggcca atattttttg tttactttga tcatttcatc tagtattttt tttgcgactc
      601 tggcatttgg tacggtttga ttcaatgtaa atgcttgtaa agctttttga taagaacctt
      661 caaagtaagc atctactagt aacttctcta ctgcaacttg tgcctccatc aatcctttat
      721 gaaaatcact gattggtttt cttagtgtaa ttggctccac accagttgca ccaacatatg
      781 ccggtatttc aacaactgca tctgaccgta gattcggaat cgccccttga ttaggaacga
      841 ttagcataaa gcgacgacga tcatcattca acaaggaaat agctatatcg acgatatatt
      901 gtccatgctc cccaaaatta aagttcaata cttcttccac aataccgcgg cgtattttat
      961 cagccatttc tttggtattt ttctctcgtc catccatgac catattagca cgtgtatact
     1021 ctttgtcagt atgctcaacg accatatcag gatataagta atactctaag taattattcg
     1081 ggatattact tggaaaattt ttcgtgatga ctgacatcat ttgaaaggtt ttttgccaag
     1141 ttttatctcc tatattaaaa tcggcgactt gcatttcttt tgttattagt ttttcaataa
     1201 tctctggcat aatttctcgt tgcaattctt tatcataaat cgatgtatac cagccaaagt
     1261 ggtttaaacc ataatacgta acgatccaat tcttacgatc ataaccgtaa ttcactgcaa
     1321 tcgtttcttc aattgaaata gtcatatcac aggcattaat catttttaca ttcggatatt
     1381 tacgacgaac agcttcagaa acgatcgact ctggatttgt ataattcaaa atccatgctt
     1441 tgggggcata gtcttgtata tgatcgacaa tttccaataa acctttgatt gagcgcatcc
     1501 cataagcaaa tcctccgagt ccacacgttt cttgtcctac taatccatgt ttaagtggaa
     1561 tcttttcgtc cttttctcgc atctccaatc ctccaacacg aatttgtgag aaaacaaaat
     1621 cgcatcctgt aaaagcaagc tttggatcat ccgttgcacg aatgcgaata tttgaaaaac
     1681 cttttctttt cagcataaat ttaacaatca agtacatatc catatttctt tcttcatcaa
     1741 tatcatataa acgtatttca gataatggca accgtttttc attgttcaaa atggcttgaa
     1801 cgattcctgg tgtatacgta ctcccgcctc cagctatagt gataatctgt tttttcatca
     1861 tcatcttttc ctttctattc ttctaataat ttattgaatt ctgccataac ataagtcact
     1921 tctaagccga tgataatttg ataatttttt tcagttggtt taatgatccc gcttatccct
     1981 aattgcttga ttcgctgttc ctcgaccatg gtgggatcca ctacctctac ccgtaggcgt
     2041 gtattacaac tatagacatc caggatattg gcttttcctc caaacgcttg tagcattttt
     2101 gttgccatat attcataatt tttacttgct aaccctaact ctgtttcttc taaggttact
     2161 tcattgccaa actctgcctt agcttcacgt cctaatgtct gaatgtttct cttattgatc
     2221 aaataataaa aagtaacgta atacaaagca aaaaagaaaa ttccgattgg aagaatcaaa
     2281 atagcattgg tagcgattcg aaaattgatg agataatcaa caatacatgc tccgaatgta
     2341 aaaccaattc gtatattgaa taagtaacag acaatccctg ctaaaccggc aaaaagtgca
     2401 tggatcacgt acaacatagg agaaagaaac atgaaactaa attcaacagg ctcagtgata
     2461 tttgcaataa atgaagttcc tgcaccgctt cccatgattc ctttgaccat ctctttattt
     2521 tctttataag ctgctttata gatagctaga catatagctg gtataccaaa catcattact
     2581 acaaaaaaaa ctgataaaaa cagtccagcc tgaggatctc cgttgataaa gcgtgtcatc
     2641 tctcctctga aaacttcctt gctaggagat gtatagcttc ctaaatcgta ataaatatac
     2701 gtattcaaca catggtgcag tccaaagggg atcagcaaac gattcaaaaa cataaatatc
     2761 cccacaccta atgctcccat atcaacgagc agttttgcaa aattatcaat cccaatttgg
     2821 atgagcggcc agataaagcc caaaaaaaca gctgcgattg tttgtagaat catcaccata
     2881 gtcaatggga atttttcgcc cgcaaaataa gaaaaaacta ctggtaattt ttgttcttta
     2941 aagcgattat acgtccatgc cgctactaaa ccggaaataa ttccaccaaa aatccccata
     3001 ttcacttctg ggtccataat ttctaagcct tttttcaagg tagcaatcgc taaaaaacca
     3061 gtaagtgcag gtattccttt gtccttattt ttgacaaaac cgattgcaac tcctatagca
     3121 aataagatat ccaagtttcc aaaaacggca tttccagctg caccaattac tgggatgttc
     3181 agcatatcgt cagctgataa acgcatgatc aatcccgcta atggcatcgc tgcaactgga
     3241 agtagcatcg ctcttccaag cttctgcaca tttgatttaa cattcatttt tattcctcct
     3301 ctattaaata tcctcatatg ttactaactc aatcaaagga tcatttttca ctttttgagc
     3361 aaatgctgaa tgaattaaaa attcgacttg gtgggtacga ggatacacaa aacttgaacc
     3421 atttaacaat tcctgatcca aatacgctgc gtgacacatg acttcgatcg ttttatcttc
     3481 tctcaatatc atgagtaaac ttgccaaata ttcttccaca ctcgtattga tttgtcgttt
     3541 tttttcatca gtttctccta cgacatcgaa acaaggctca aaataatcga catgacgaac
     3601 ttcatctttt cgttcaaagt tgctccgaac cggcaaatca tatttttttg ctaatgtaac
     3661 aactacctcc tgattttcat ccaaagtatg agtatgatga tgactgtcta aatgagttgg
     3721 tatgatcccc gcatgataaa ttttttgaat ctgagcattc cattcctgat acagttgatc
     3781 tttgtcaatg ttaaatggat gctgataata agataaggat ctgaactctc cgctttcgat
     3841 aagagtatcg acagttttga ggagtggttt gccacacgtc aatgttaaat ggactcctac
     3901 acctagccta ggcatttcct ttcttagttt cacagcatgc tcgaaacccg gcatatttgc
     3961 catcaatgta gttgatgtca aaattcctcg ctgataagca tccataatcc cataattcac
     4021 accatggcta tagccaaaat catccgaatt aaaaatcact tttcccattt cattctcctt
     4081 cttttttcta ctaaaatagg cgcaaccaaa taatcatagt ccatttacta tgtgtcattt
     4141 ggttgcgcct atagatagtt acgcccttat tttaatagat tcttgctaga tgaattgcca
     4201 aatagatgat ttcattttct tgaatggcaa gttgaaattg ctgttcgatg atatctttaa
     4261 tctttagtgc tgtacaatac gctttgctat gtttaaatgt ttcagtaaaa aaaccaatat
     4321 tttctactgg cgtttcttcc tgataaatca agcgtttaac caggaatttg atatgaacca
     4381 ttaagcgatg ataagtcagt gattctttat cataaacgtt tgaataggat ttttctagat
     4441 gggacaaaat cgtggtcgtc atttctacta tttttaagct ctttaaacta ttaggctctc
     4501 ctagctcagc attaatgata tgcatagtga tgaatcctac ttcatttgac cctaactcgg
     4561 tttgatatcg atgattgatt cgctcgacca cttctactgc tatatcatat tcatttggat
     4621 aaaggaatct catttctggc aacataattg ctgtgacatc tctatcttct ctttttcgtt
     4681 gaatagcaaa ataaatatga tctgtcaatg taatatagat atttgaatct aatggagagg
     4741 agatcctagc tgttatcagt tcaatcactt cttccgcaaa atccataaat tcgattggaa
     4801 tctgcgagac tatctcactg aatcgtttga tcttttcttt agaatctaat acaaataatt
     4861 tgtcagcatc ctcaacatca aatggcttac tcactttagc agaaaagcca atccctttcc
     4921 ccatcgcaat tttttctatt ccatcgtcat cgatcaatac agcattatga ttcagctttt
     4981 tcatcacata caccgttaat tcacctcctc tatatgataa aacaaaacaa cttgcagact
     5041 ccttcataca tatttttgtc tccaaacaat ttttaaagac aaaaaacgac ctaagaaaga
     5101 accctttctt aggtcgtgcc taattgaata gtagcaccct acataattta caaaaatagt
     5161 ttaacacata ataaaagcgt tatcaatatc ttttttactc gttttatgat aatacttgta
     5221 tcgaattgat ttggaggtaa ttacataaaa acaaaaaaaa tatagcaact ctctataaga
     5281 aagttgctat attgataatt ataccggcgg ccggggtcga accggcacgt cctcaacgga
     5341 cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaaat ttaaaaataa
     5401 taactggggt agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg
     5461 cttggctata ccccaagaat aaaaggcgaa tgatgggaat cgaacccacg aatgccggag
     5521 ccacaatccg gtgcgttaac cacttcgcca cattcgccat cataaaagat gttatttaat
     5581 ttctcaataa aaaactaaga tgaagacatc ttagtcaata ccggcggccg gggtcgaacc
     5641 ggcacgccct caacgggcac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     5701 ggcaagttga tttattagta ctaaggcggt aaccggattt gaaccggtga tgaaggtttt
     5761 gcagacctct gccttaccac ttggctatac cgccattata ctattatcaa tctataaaat
     5821 aaagaataaa ttatagaaac tggggtagct ggattcgaac caacgcatga gggagtcaaa
     5881 gtcccttgcc ttaccgcttg gctatacccc aagaataaaa agggcgaatg atgggaatcg
     5941 aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca ttcgccatgg
     6001 caggggcagc aggaattgaa cccacactaa cggttttgga gaccgctgtt ctacctttaa
     6061 actatgcccc tataaaatgg aggagagtgg attcgaacca ctgaacccta aggaacggat
     6121 ttacagtccg tcgcgtttag ccacttcgct actcctccat ggtggcgcgg gacagaatcg
     6181 aactgccgac acacggagct tcaatccgtt gctctaccaa ctgagctacc gcgccatatt
     6241 ttaaaattat taattgaata acaagaacga cggttccgac gggaatcgaa cccgcgatct
     6301 cctgcgtgac aggcaggcat gttaacccct acaccacgga accagttcat tatggaggtt
     6361 aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa
     6421 cctccaacat tcaattaaaa tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa
     6481 gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaat ttgctacgga gagtaaggga
     6541 ttcgaaccct tgagacagtt tggcaccatc tacatgattt ccaatcatgc tccttcggcc
     6601 tctcggacaa ctctccagag tgaaagaagc tcaatactct ttctcaaaat gagaactccg
     6661 gcagtaggac tcgaacctac gacatcatga ttaacagtca tgcgctacta ccaactgagc
     6721 tatgccggaa taacat
//
LOCUS       DASJUF010000127         6724 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.129, whole genome shotgun sequence.
ACCESSION   DASJUF010000127 DASJUF010000000
VERSION     DASJUF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.129"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            91..1098
                     /locus_tag="U4B62_002506"
     CDS             91..1098
                     /locus_tag="U4B62_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2142616.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1110..2114
                     /gene="neuB"
                     /locus_tag="U4B62_002507"
     CDS             1110..2114
                     /gene="neuB"
                     /locus_tag="U4B62_002507"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEN2142617.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2111..3259
                     /gene="neuC"
                     /locus_tag="U4B62_002508"
     CDS             2111..3259
                     /gene="neuC"
                     /locus_tag="U4B62_002508"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN2142618.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3232..3909
                     /locus_tag="U4B62_002509"
     CDS             3232..3909
                     /locus_tag="U4B62_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2142619.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3899..5041
                     /locus_tag="U4B62_002510"
     CDS             3899..5041
                     /locus_tag="U4B62_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN2142620.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5054..6031
                     /locus_tag="U4B62_002511"
     CDS             5054..6031
                     /locus_tag="U4B62_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2142621.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6031..>6724
                     /locus_tag="U4B62_002512"
     CDS             6031..>6724
                     /locus_tag="U4B62_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2142622.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRK"
BASE COUNT         2512 a          835 c         1276 g         2101 t
ORIGIN      
        1 acatcattat tgataattag tttagttata tattttttaa agattaagaa tcgaattata
       61 gatgactata attcatagga ggagatataa gtgtttaaag gaaaaacaat attagtaact
      121 ggcggaactg gttccattgg aagtgaaatt gttcgtcagc ttttaaaata tgaaccaaaa
      181 gctatcagaa tttatagtag aggtgaagaa aaacaatttt atatgcaaca agagctctca
      241 aatcacacta atttgagatt tttaattggt gatatcagag actctcaaag gattgattat
      301 gcatgtcgtg atgtggatat tatttttcat gctgccgcaa tgaaacacgt tccagcaagt
      361 gaatataatc ctatggaagc tgtaaaaaca aatgtgatgg gaactcaaaa tgtaatagat
      421 gcagcgatca aaaataatgt aaaacatttt gtaggtatca gtacagataa agtagtgagt
      481 cctactaaca ccatgggtgc gacaaagtta ttgagtgaaa aattaataat agctgccaat
      541 cagtataaag gaaaaacaga aacaatattt tcttgtgtaa ggtttggaaa tgtcatgggg
      601 tcaagtggat ctgttattcc tctttttagt aaacaaataa aaaacaataa agatattact
      661 ttgacagatg ttgatatgac acgatttatg atgtcaatac cggatgctgc aagattagtt
      721 ctgtctgctg ctatgaattc aaaatttgga gaaacatatg tcttaaaaat gccggcaatc
      781 aatattttaa ctttagctca atcattaata aaaatatata atgatacaca tgatgaaaaa
      841 tatagaggtg aaataaaagt tgtaggtatc aggccaggag aaaaattata tgaagagcta
      901 atgactacag acgaaattga acgctcatat gaaaatgata aaatgtatat cattccacca
      961 cactatcatg ctactgacga actgtacaaa gattatgaaa aaattaaaat cactgcatac
     1021 tcttcagatg caacagaatt aatgagtgga caggaattta ttagaatcgc aaaagagaat
     1081 aatgtcgtga cttggtaagg agttttatga tgaaatcaga agtatatgta attgcagaaa
     1141 ttggtgtaaa ccataatgga agtctagatt tagctttaga aagtattgat aaagctaaag
     1201 aatgtggagc taacgcggtt aaatttcaaa catttaaaac gaataagcta gtttccaaaa
     1261 aggctccaat ggcaaaatat cagaaggata atcttaaaga ggacagttct caatttgaaa
     1321 tgctaaaaaa attagaatta tctcaatctg attttattag aataaaagat tattgtatac
     1381 aaaataaaat tgattttttg tctactcctt ttgatgaaga aagtgcagac tttctaaaac
     1441 aaattggtgt acaaggattt aaaattggct caggagattt aacaaattta ccttttctta
     1501 gaaaaataga tatgtatggg ttgccagttt tattatctac aggtatggct gatattcatg
     1561 aggtcagtga ggctgtagat tgtttcatag atagtccagt tactatttta cattgtactt
     1621 caaattatcc ggctagttct gaagatatta atttattggc gattaacaca ttaaaagaaa
     1681 tgttcgggat acccgttggg tactccgatc attcattggg atatgatgtt ggtatttgtg
     1741 ctgttgcgat gggtgctaag gtaattgaga aacattttac cttggataag gaactacctg
     1801 gtccagatca taaagcttct ttagatcctc aggaatttgc agattttgta aagcatatta
     1861 gaaacactga aatattttta ggtgatggtg ttaagcgagc aatgccttct gaacttagta
     1921 caaaagaagt atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg ggagatattc
     1981 ttacagagaa atctcttaca ataaaacgtc ctggaaatgg aatagctcct aagtttttat
     2041 atcaatatat tggcaaaaaa gttaaaagac caataagtgc agatagtgtt ctgcttgagg
     2101 aagatgttga atgaaaaaaa tacttatagt aagtggaaca cgagctgatt atggaatata
     2161 taaacctgtt gctgagaatt tactacaaga taattgggat gtatcatttg ttgtttctgg
     2221 aatgcattta tcccataaat atggatttac taaagaattg atcttagctg acggatttag
     2281 aattagtgga gaagttcaaa gtttgttttt agagaacaat aagggaaaca tggctcggtc
     2341 tgtggcatta gagataaatg gtttcacaaa tatatttgaa caaactagcc ctgattttgt
     2401 attattacta ggggatagag gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg
     2461 aatcaaaaca gcacatattc acggaggcga agtgtctggt actgtagatg aatcaataag
     2521 acatgccata acgaaaatag catctgttca ttttgctgct acggaaaaaa gcaaagagcg
     2581 tttaataaaa atgggggaag attcttggag agtttttccc gtcggagctc ctagaataga
     2641 tactatttta aataatagat tacctagttt tagagatata aaagaaaaat atcatctaaa
     2701 atttgaaaag aaaaaatacg ttttgcaagt atttcatcca gttgtaaccg aactagaaaa
     2761 tatagaaaaa caaattttag ctttgatcaa ttcaatggaa caacaatctc aaccaattat
     2821 ttgcatttta cctaatagtg atgctggtag cgaaattatc agaaaaatgt accagaaata
     2881 tttaaaaacg gatattattt atattacgaa tttagagccg gaggaatacc tgactatatt
     2941 aaaagagtgc aaatttatga tcggaaattc ttcttctggg ataattgaag ctgcatcatt
     3001 caaaatacca gtaataaatt taggaaccag acaaaatgga cgagaacaat ctaaaaatat
     3061 tataaatgca aatctaaatg aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc
     3121 tcagtttata aaaagcttga aaaatgttaa gaacatctac ggtgacggca acagttctag
     3181 aagaataaca aaaacattga atcaaatatt gaatgatggg gaagatatta agtggattca
     3241 aaaacaaata gcttattaaa atatactagt gatccataca ttattattgg ggctggcgga
     3301 cacgcaaaag tcattattga aaccttaaga ttagaaaata agttaatata tggatttttt
     3361 gatgataagc aaaataaaat gcaagatctg aaatatcttg ggaaaataaa cgaattgaat
     3421 agctgggcgg atagcaaaaa aaaaatctat aaatttattg tagctattgg tgactgtgta
     3481 gtaagaaaaa agattataaa aaaattgaat atgccaagtg agcaatatgg aatagctgtt
     3541 catccaagtg ctatagtggc aaaagaaatt tcaattggtt atggaactgt tatttttgcg
     3601 aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg ctataataaa cacaggtagc
     3661 attgttgagc atgattgtag aattaataac tatgttcatt tatctccagg tgtatgctta
     3721 gcgggtggag tacatgtagg tgtaggaact caaataggaa ttggttctca atgtatccag
     3781 atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga gcactattgt taaggatatt
     3841 ccctcacatt ccttagctta tggcaatcca gctaaaatta agaaagaagg aattaatttt
     3901 gaattctaga attttattat caagtcctca tatgggagga acagaagaaa aatatgttgc
     3961 taaggctttt aaagataatt ggatagcgcc attaggtgaa aatgtggata aatttgaaga
     4021 aagtattaca aaatatactg gttctaaggg cggagcttct gcgttaagtt caggtacagc
     4081 tgccatacat cttgccttaa gactgcttaa tgttggaaaa aacgatattg tctttgctcc
     4141 ttcatttact tttatagcta cagtaaatcc tattttatat caaggggctg taccagtgtt
     4201 cattgactca gaacctaata catggaatat gagccctgtt gcactagaga gcgcattaga
     4261 agaagctgaa agaaacaacc aattacctaa ggcaatcatc gtagtgaatt tatatggtca
     4321 aatggctgat tatgaacgaa ttttagctat tgcaaaccaa tttaatgttc cagtgataga
     4381 agatgcagcg gaatcgttag gttctactta taagggaatt tcgtcaggct tatttgggac
     4441 aattgggatc tattcattta acggtaataa gatcatcaca acctcaggtg gaggagctct
     4501 agtgtctagt gataaaaaat atgttgagca ttcaagattt ttagcaacac aagcaagaga
     4561 tcaagcaccg tattatcaac atagcgaaat aggttataat taccgtctaa gcaatatttc
     4621 tgccggtatc ggacgtggac aaatggaagt attgaatgat agagtagcta aacgaagaga
     4681 aattttcaat cgatataagg aagctttttc tggaattgat agaattgaaa tgatgccaga
     4741 agtggatggc atggtgccaa atcgttggtt gtctacttta acgatcaatg attataattc
     4801 tattgatcaa gtagttaacg taataaataa actaaatgaa aaaaacattg aaacaagagc
     4861 tttgtggaaa ccactacatt tgcaaccagt atttcaaggg gtaaaaatgt attaccatga
     4921 taataatctt agtgtgtcag aaaagttatt taatagcggc ttatgcttac cttctggaag
     4981 taatatgaca gatagtgaac aagaatttgt tattgatgag tttaaaaaaa tattcaagta
     5041 ggtgtgatat tttatgaaca taggaattat tggtttaggt tccatgggga aaagaagact
     5101 tagactcatt caagaacact ttaatgatat tttactatgt ggtgttgata ctaatgaggc
     5161 gagacgaaag gaagttgaaa aggaatttgg aataccaact tataatagtc taacagaggc
     5221 aaaaaatttg tttgaaataa attcagtttt tgtttgtaca tcccctataa cccatgaatt
     5281 tattataaaa gaggctttaa atttagattg taatatcttt acggaaatca atttattaaa
     5341 tgaatattat gatgaaataa tcgaagtagc taaaaagaaa gatagattac tttatctatc
     5401 ttctacattt ttgaagagaa aagaaattca atatattaag tctaaagtga cgcataataa
     5461 aaaaatttct tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga
     5521 ttatcgcaat ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat
     5581 tgaattacct tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca
     5641 gaaattatcc aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga
     5701 aactggtata gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt
     5761 aactataatt ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc
     5821 atggaatgaa gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga
     5881 taataattat tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt
     5941 cacgcttttg gaaaagaaaa tggaacccca atatactttt gaaaaagata agtatattat
     6001 taacttaatt aacgacattg aggtaaatta atgatgaaag taattttttt tgggttaggg
     6061 tcaattggaa agagacatct aaaaaatcta ataagctatg gaaaaaagaa ttctattaat
     6121 tttgatataa cagcttatag tagcagtgga aaaaaagagt ttgaagaaat aacgtatatt
     6181 caaaatagag acgatttgga tacacattat gatatagctt ttattacaaa tccgactttt
     6241 aagcatctgg aaacactaaa tgtaattgtt gaaaaagttg attacatatt tttagaaaaa
     6301 cctgcttttt ctgagagtat tgatcttact caatataatt atgattacaa aaatatttat
     6361 gttgctgctc cactgaggta taaaaatatc atggaagttg ttcgtaatag tattcaaagt
     6421 aaaaaagtat attctgtaag agctatttgt tctacttatc ttcctgattg gagaaaaggt
     6481 actgattata gaaataatta ttctgccttt aaatctatgg ggggaggagt agaattagac
     6541 tgtatacatg aattggatta tattataaac ctatttggtt tccctaaatc agtaaaaaaa
     6601 cagtttggta aatattcgaa tcttgaaatt aacagtaatg atttagcaac atatatattg
     6661 agatacgatg ataaaattat agaaattcat ttagattatt ttggaagagt tcctacaaga
     6721 aaat
//
LOCUS       DASJUF010000128         6324 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJUF010000128 DASJUF010000000
VERSION     DASJUF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.130"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U4B62_002513"
     CDS             complement(99..1229)
                     /locus_tag="U4B62_002513"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN2142623.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="U4B62_002514"
     CDS             complement(1878..3080)
                     /locus_tag="U4B62_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2142624.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="U4B62_002515"
     CDS             complement(3140..3343)
                     /locus_tag="U4B62_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN2142625.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="U4B62_002516"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="U4B62_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="U4B62_002517"
     CDS             4880..5692
                     /locus_tag="U4B62_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142626.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1881 a         1299 c          997 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaa
//
LOCUS       DASJUF010000129         6280 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJUF010000129 DASJUF010000000
VERSION     DASJUF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.131"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            8..346
                     /locus_tag="U4B62_002518"
     CDS             8..346
                     /locus_tag="U4B62_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142627.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            456..677
                     /locus_tag="U4B62_002519"
     CDS             456..677
                     /locus_tag="U4B62_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142628.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            697..2853
                     /locus_tag="U4B62_002520"
     CDS             697..2853
                     /locus_tag="U4B62_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142629.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            2865..3098
                     /locus_tag="U4B62_002521"
     CDS             2865..3098
                     /locus_tag="U4B62_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2142630.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3088..3954
                     /locus_tag="U4B62_002522"
     CDS             3088..3954
                     /locus_tag="U4B62_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142631.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            3969..5939
                     /locus_tag="U4B62_002523"
     CDS             3969..5939
                     /locus_tag="U4B62_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142632.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            6106..>6280
                     /locus_tag="U4B62_002524"
     CDS             6106..>6280
                     /locus_tag="U4B62_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142633.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         2412 a          867 c         1231 g         1770 t
ORIGIN      
        1 tttttatatg ttcgattcta ttaaaaattt ggttagcgga atgacgcctg atatgaaaac
       61 tttagtacaa gttgttttgt atttcttggc attcgttttt tcgttgaaac caggattcac
      121 tgcaatgaaa ttttttggtg atgcaaaatg gggagatgga ttaaaatatg ttggggcgat
      181 tattgcaatc tttattattc ctgtaattac aaccgttatg ctttacacaa tgggtactaa
      241 aacaggtaat gatttgaacc aaaaagctaa tatcatatca gcacttattc cagttgttgg
      301 tatctatcta gtttcaaaat ttccaaaaaa tgaactgtta ggttaaaagt gaattgaaca
      361 aatttggata atatgccgat ttgttcaatt tactttttac caaaaaacta cgctccaagc
      421 gtagtttaga agtgagggaa gaaagtaaat gtaagatggt ttttgataga gaaaaaatgt
      481 tagctcacgc caacgaagta ttaatgagca gtctgaaagg taccgaactg gcaaagataa
      541 tgaatatgaa tgttaaccag ttctatgatt atcgaaatgg gagtaaaaag attgaaaaag
      601 ccagacttga aacgcttatt aaattcgaaa aagcatatgt atatatgcta gataaacaga
      661 aaagaactat tgactgaaaa aaggagtttt acaataatgg caaaattcaa agatattacc
      721 gaatctatta attatgtggc agaaacagtt cctgtagttg cactctcttt gaagccacaa
      781 ttactcgaag tgtacaaaaa agaagaaaaa gaagcgaagg caaaaaaagc taaagcaaag
      841 gtatggaaag aattcgatgc gttgctgacc aaagatttac caaagaaaaa ttatatggta
      901 attgaagatg tacaaaagct caacagtgag ggagttatag atgaggatga tgctattctc
      961 gattctgaga agtatgtcgt tctattgaag tcaagtttga attatgtagc tgatctacca
     1021 gaaaaattta aagagaagct attagcaggt gtccgtttta tggatagtag tggtcaatta
     1081 ttagttattc cggatgatcc taatgtgttt gaatcagaag ttgaagaagc tacagacttc
     1141 tatagcgcag tagagcaatt agaagtagct actaatggat tagaacgaat tgctgagacc
     1201 ttcaactctt ctgaagaagt tgaatttgaa gccgtagcag aaaatgtacc agatgaaaaa
     1261 gaagctcttt ttgtggatga taacaaacca accgaagcag aaaaccaaga agtaagcata
     1321 gaagaaacac aaatacaaac agaaacctac caacaaccaa aaattgatga aaaacccgaa
     1381 tcccaaagtg acaaagaaaa ttatgtttat gaagctgaaa aaatcacatc tgaaagtgat
     1441 gtaccgaaaa acttagatgc tgatttttta gaaaaaaatt ttgaacagat cattgctgac
     1501 aaatttcaac ctgtagtttt agatgagatt aatgaccctg tcgatttaaa cgaggagatg
     1561 ctatccatac ctgagttgaa accattgctg tttttagcta aagagagtat gaacgctcgc
     1621 atctctattg ctaataataa aatcaacagt gaattaaata agacgaagga gcaattgatc
     1681 ctcaaaggaa agcaaatgtt agctgatgag cttttgcgga tagaggaagc aacctcatta
     1741 aatggtcaaa atcagtttac aaatacacta tcggttgcta aagagagcta cgaagcagaa
     1801 gcatctaaaa ttgatgattt agtgatggaa aaatccaata tcattcgtaa agattatgat
     1861 atggcgaaac aggcttattt ggatgctgaa ctaaagaaac tagagcaacg gtatgatgct
     1921 gagcatttac cagaagttaa acaacaagaa gatgaatata gaaaagcaac tgaagaaagt
     1981 cttgatcaac agtaccaaga agcaattgaa tctattaaaa acgatgcatt gaaacaaagc
     2041 caagaagcaa aagaagttgt ggtggaacga attatcaatt cattgaaacc agaaattgaa
     2101 aaatcattaa cagacgtgca agaaaaaact ttagagattg tcgaaaaaac aaagtcagaa
     2161 aatgaagctg agttccatga tacaaaagag aaaacaacag atgtggttgt caaatatttt
     2221 gcgaacaaag aagaagttga aaagcaggta gctaacaaca cgaaagaatt agaaacaaaa
     2281 cttgtagagc aacaaaaaac tatcgaagaa tataaagatc gaatgaaaga ggtcaaagat
     2341 ttagcagagg gttataaatt aaatagcgaa aagttggcag aacaaagtga agctttaaga
     2401 gaagacttat ctcgttcccg aagtgatcta gctcaaatgg cagaaaaaac gaaatcatta
     2461 gcagttgcta cagaaaatca atcaagttca aaacgtgatt ttacaagtaa aaagatggct
     2521 actatcattt ctgtcgctgc aatggcttgc ggaacgatct tgggatcgtc ctactttatt
     2581 agtcataata atcaacaagc acaggctcaa actcaaacat ctatttcgag agtggaaaat
     2641 tcgattggga caaatacgaa ctctgcttca acaaatagta gctatgaagc aacaccaact
     2701 acagagttca aagttgggaa agatgttgtc atcagtattg atgggaaaac aactaaggct
     2761 gaagtgattt ctattgatgg agaaaaagca gttgtgaaaa ctccagacgg aaatcagtac
     2821 aaaattcaag ctcacttatc tgataataac taaggagggc tattatgcga gaagaaaaaa
     2881 tagtttatcc agtaattcaa aacaatcaag aaaacatgac ctcttctcag cacttacagg
     2941 aagcacagct cgtgtatcag gtaaatgaac taccacaaac gaacgagaca atgagcaagt
     3001 caatatcttt aattggcttg cttattgttg caatagtgat aactgtaggt gtttaccgta
     3061 agttgaacgt gaaaggaagt atttctgatg gcagataact caaactatgg atttaaacat
     3121 aacttacatc aaaaaccttc tgaaacaatt gtcagagttg aatggcttga aaagcatgtc
     3181 aatagcacct attctgttac agcaaaaaga ttaattgtat cagctatagt tgttgcagtc
     3241 gttgttcttg gatcaattgt ttacttagtt tctgatacga ttaaactgaa atttattttt
     3301 gttccaacgt tattaatgat aattggattt cttattctaa ttagccagta tatgtttatt
     3361 gatccatcta gtggttcatt gatttctggt tctattagac tgttcaaatc agtagttaat
     3421 tcagcaagag tatctatggg gaaaaagaaa ccatttcgag gcacagagat tttaaaagtg
     3481 aaacaagatg gaacaatcgt atatcgagat gggagtgtag cattaatgtt caatctagat
     3541 ggggctacat cactaaccgc ttttccagaa gaagttatgg ctcaggaatc gaaatcaata
     3601 caatatcaaa ataccagaga tcgttcctta gctgaaatca aaatcacttc atctcaacct
     3661 cagaatacgg aaagacaaac tcgaaatatg aaaatacaaa aacgaaatgc aaggagtaac
     3721 aaagcagtct ttgacataat agatcagcaa gaacgatttg tatcagaata tattgatggt
     3781 gtgaaaacaa cagtaatcca atatcttatc ttgaaatctc aatctaagat ggtattggat
     3841 gaaggtgtgg aacgtttaga aaactatacg agtagaggac tttattatag tgtcactcag
     3901 ttgtcagcta aacaaacaaa agaaatatta tctgatattt atgcactgaa atagaggtga
     3961 gagattaaat ggaaattatg ggtagatcac aactattaaa tttcaaaaaa aagagaaaaa
     4021 ctaaaaaaga acaaaaaact agaaattcaa aggtagtgaa taataccttt gttagtgatt
     4081 taacatcttt agttggttat caatttacac catcttacat taggacaggt aagcggtggg
     4141 gaacaattgt gaaattagtg aatacttatg gaatgaatag aaattatgga tttggttgga
     4201 tggttaaagc tattcctgaa gtgagagtag aaggggtaaa agcctatcta attgaagcag
     4261 ataagccagt gtcacaaaaa gatcaacaag aaataatgca cgagaatgtt gggggatcaa
     4321 cgaatagtta tgaaaataat ttagaaggaa aaaattcaga tgctgaaatt agcaaaactg
     4381 ttatgcggat tatggatttg aatacagcat ctttactaga cagcatggca aataaaatta
     4441 ttgattggaa gctagaaatc ctattagtat ccgattctcc taataaaatc agcgaacagc
     4501 taattaaact ccaacggttg tatgatgaaa aaatgtatgg attaaagctt atgtcatatg
     4561 gaggagatca acgtgatctg tttgatagac ttttaagtga accaatcggg aatgtctatg
     4621 agtatacgtc tatgagttcg gattttgcag gaaatgatca tgcaattcgc aaaggtttag
     4681 atgatgcgaa tggatgggct atcggcacta tgtctactag ctacgcaagt ggtgtagctt
     4741 ttatggcact ggatgattct tttacaagta aagttttagt ggcatcacac aaaaacgcta
     4801 caattaaagg ctatgatgaa aaaatctcag catcatcact ttgggggcaa ttgattgcta
     4861 ataatgcaat ggcaaatcat aaacgaactt ttcatattgt cttaaataaa tttgattact
     4921 ttggtgatcc aactggtaga acatttgttg ccaaaccttc gttaaacagt gtaatagaaa
     4981 aggttaattt ggctgaagaa ggtcttaact ctttggagct ttttggtaat caaaaggaca
     5041 ttggtgattt atatagacgg aatctcgata aactaactca gattttttat ttgttagcag
     5101 gcagaacatt agattctgca actagagttt tattgaaaaa agaattaggt aacttttata
     5161 taagtcagaa actttgggat attaattatg aagaacgttc aaaacttttg gtgaacagta
     5221 gtgatacaat gccagttctt ggggatttta ttagctattt gactaatgct gaaacaaaag
     5281 ccaaaaaatc tggtactgaa aaagaagtcg atcgtattat tgtattaaaa gacacattga
     5341 gaacagcatt agacagcttc tctaggatgt ttaatcaaag aacgacactg aaagaaatcg
     5401 gtaaaagtga caaattacag ttttactata acttaggtga tctaagagca gaaaccaatg
     5461 ttatggaagc tcaattttta aatacctttg atttcatcac atcacaagca aaaaaagatg
     5521 atattgttat gattcacggc ttcgataagt tatctattga aaccgttggg ctaattaaaa
     5581 aaagattaac aatgcttgaa gaagatggaa ttcgtcttgc ctttctattt gatacaatcg
     5641 gttcaggaga agacaatgaa tacaaagaag atattgaaac agctgatata ttcaatacag
     5701 atggaaaaat ttaccaaagt tttgaaagtg attttaatta cactatcatt ggaaagatga
     5761 ccagtggaga gttaatgaag tatgaaaaaa aagttgctcc tgctagaaaa ggagaagatg
     5821 atcaaaaact ttctgttgat ttgagacagt atttgacaac aggaagaagt aataaatttc
     5881 agatcaggag accagtggat ctgactaata attttgttat tggagagttt attctttaag
     5941 ttttatcttt gattttgagt gtataaggaa aggataagaa aaaacatgta tatttgagac
     6001 taaaaacagt cttagagaac atggtacgtt ttgtgaaaag agaactatag aaatttcata
     6061 aagtgaaacc ttactgagtg aaataattat agtggaggtt aaataatgaa accaaacata
     6121 aaaattttag atcgaatatt tctaggtaga gacacagaag taatcctaat tcaacatgaa
     6181 gaaggatttg aagttagtat aggtattcag aaacttcaaa aacctcatta ttgtaatcag
     6241 ttgtataaaa actttactga tgaagaaaaa gcgagagttt
//
LOCUS       DASJUF010000130         6248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.132, whole genome shotgun sequence.
ACCESSION   DASJUF010000130 DASJUF010000000
VERSION     DASJUF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.132"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            15..1391
                     /locus_tag="U4B62_002525"
     CDS             15..1391
                     /locus_tag="U4B62_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2142634.1"
                     /translation="MNHKNSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIK
                     IMEEPAGYSKEKINGIMKKYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLT
                     VPTDPIPYIKSIITEGTGGLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLV
                     ERQKISPNITDLFDEKIAIHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQ
                     SPRISDDSAYKETLKALQSNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNR
                     RKELQTYMQGLHYTDTAVKKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQH
                     QTDYFIYNNKYSREQAATLNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPAL
                     TIDPTESLSNRFNGKQVFVTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSAT
                     WATQKIKD"
     gene            complement(1451..2167)
                     /locus_tag="U4B62_002526"
     CDS             complement(1451..2167)
                     /locus_tag="U4B62_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN2142635.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2394..2936)
                     /locus_tag="U4B62_002527"
     CDS             complement(2394..2936)
                     /locus_tag="U4B62_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2142636.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(2941..3168)
                     /locus_tag="U4B62_002528"
     CDS             complement(2941..3168)
                     /locus_tag="U4B62_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2142637.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3247..5127)
                     /locus_tag="U4B62_002529"
     CDS             complement(3247..5127)
                     /locus_tag="U4B62_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2142638.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5316..5930)
                     /locus_tag="U4B62_002530"
     CDS             complement(5316..5930)
                     /locus_tag="U4B62_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2142639.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6072..>6248
                     /locus_tag="U4B62_002531"
     CDS             6072..>6248
                     /locus_tag="U4B62_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2142640.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         1894 a         1278 c         1036 g         2040 t
ORIGIN      
        1 cgggtgtctt tttcattaat cacaaaaatt cgccttcttt cttgctgttt aatttattta
       61 aagtaaacaa gactttcttt aaccagcaag gcgccgtaaa agataacgga ccagtcattc
      121 aattcctaaa caatatcgac attaaaatca tggaagaacc tgctggctat tcgaaagaaa
      181 aaattaatgg aataatgaaa aagtacaaca caaaggcaga tgaaatcaac caaacaagaa
      241 gtgactggtt aaacaatcag acgatcgttc tcaatttaag tgaaagcttc agtgatcctg
      301 cacgtgtccc taatctgaca gtacctacag accctattcc ttacatcaaa agcattataa
      361 cagaaggaac aggaggattg atgttatccg tcggttatgg tggcggaaca gctaacatgg
      421 aatgggaagg actgacaggc ttgagtatca gtaacctgtc tgcttcgtta gttacacctt
      481 atacccagct agtggagcgt cagaaaataa gtccaaatat taccgatcta ttcgatgaaa
      541 aaatagccat ccatcctttt acagctgctc tttatcgcag aaaacaagtg tttgataaat
      601 ttggttttga caggttctat tatttagaca gtccggataa attaacttat acggataaaa
      661 tagaacaaag cccacgcata tcggatgatt cagcatacaa agaaaccttg aaagctttac
      721 aaagcaatac ggaaacgtca caattcatcc aattatccac tatgcaaaac catatgcctt
      781 atgatgatta ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc attcctaata
      841 gaagaaaaga attacaaact tatatgcaag gccttcacta taccgataca gcagtaaaaa
      901 aattcatcca agaaatcgac aaaatcgata aaccgattac ctttgttttt tatggtgacc
      961 atttacccgc aatctatagt gggaatgatt cgaaaaaata tggattagaa cagcaccaga
     1021 cagattattt catttataac aacaagtata gtcgtgaaca agcagctacg ttaaataaag
     1081 aagttgttgc tcctaataac tttggtgcta tggctcttga gcatgcgaat atcaaagtaa
     1141 ctccttacta tgcgttgttg acagaagtat ctaatcatct acctgcatta acaattgatc
     1201 ctacagaaag cttgtctaat cgttttaatg gaaaacaggt ctttgttaca gaggatagtc
     1261 aaattatcca agaaaaaaat ttaactgaga aacaaaaaga aattctagaa gattatcgtc
     1321 tgatacaata tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa
     1381 taaaagacta acaaataaac tgagcaaggg gttgtgataa aagacatgtc acaacccctt
     1441 gtttatctca ttaatttgct tgtccataaa cgatttgatt aaagccattt ttcaattcat
     1501 tattcgctgt ctcgttgaag taaagataat tatcgggatt cccctgattt tgttgtgtca
     1561 ccaatacttt tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg
     1621 gttctgtctc tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac
     1681 tttctgacca ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca
     1741 aaatagcttg agggacttta agaccatata agtccacact catttgattc gtataacttt
     1801 tgtattgttg tcccattgtt tggccccatt tggtcataaa tgcctctaat tgtgcggatt
     1861 tgtctgcatt ccacagtgtt tgttcaactt gttcgctgct ttctgaagag gaagatgaag
     1921 cctctacaga agaacttgaa gtagatgttt gagttgtttc agtcgttttg gaagactctg
     1981 acgaagtaga ggtatctgct gtttctgtac ttgctgcagt tgacttgctc tctgtgctag
     2041 ttgttgtagt agttgcaact gttgctttcg ctgtttcttt tgttgattct tttttcgatt
     2101 cattcccgtt ggtacaacca gctaacaaaa aagctagacc aattgttact aaaatccttt
     2161 ttttcaatta aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat
     2221 gtgattttat tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga
     2281 agcacccgct gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt
     2341 gattttccaa tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca
     2401 tcatcgtctt cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg
     2461 tagatgattt ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt
     2521 tcttcttttt cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct
     2581 tttgcaaagt tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa
     2641 atttttccat aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact
     2701 aactcatgag agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg
     2761 gtacttaatt cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt
     2821 tcttctaatt taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga
     2881 tggtcgatat ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat
     2941 ttacgcctct tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc
     3001 taattgttca gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa
     3061 caaaactttc gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat
     3121 acagcttgga caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc
     3181 ttcctttttc ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat
     3241 caagttttac gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt
     3301 aaagataacg atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa
     3361 cccggcaaat aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc
     3421 acgggtattt aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt
     3481 agattgcatc aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc
     3541 aatcccgata tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc
     3601 aacgcgcaat ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa
     3661 ttcagcatgg acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt
     3721 atcacctgtc agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc
     3781 agcgacttct ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat
     3841 tgctacaata atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt
     3901 tggtgtgaga gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg
     3961 accatcaatc tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa
     4021 ttgttgaaaa tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg
     4081 atcggataac gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata
     4141 gttcttgata gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac
     4201 aaaagtatcg attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg
     4261 tttcgctcca ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc
     4321 aggacaacca agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac
     4381 aaccaacgca ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt
     4441 ttccgcatgg gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt
     4501 cgtatcatct ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc
     4561 agagaagact tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc
     4621 ttcatccgca taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt
     4681 ttttactaag acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc
     4741 atcttcttgg acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc
     4801 ccgcgttttg gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac
     4861 gatagctgac tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag
     4921 taaatcaatt gagataactt tgactttcac tgcctgatac gcatggatca taataggtat
     4981 gaccgcaata atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa
     5041 ttttccgata aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg
     5101 ctcttggatc cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga
     5161 ctaagtatag atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg
     5221 cggttttctt ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt
     5281 gatcttgcca actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc
     5341 atacatcgga gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt
     5401 ctttcaaatc cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat
     5461 atttcttttc ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt
     5521 gttttcaaat caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt
     5581 aaatatgctg caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca
     5641 aatatatttt gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt
     5701 ttcttttttt ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac
     5761 aaagcaagaa tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga
     5821 ttcagcagaa gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag
     5881 atagcttttt tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct
     5941 cctcaacttc aatgattgtg ttatacttac attataatag acacagtgtc tactatctat
     6001 aatcaatcat ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag
     6061 gagataggta aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg
     6121 aaacactatc cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa
     6181 cagaaacggc agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc
     6241 tatatgat
//
LOCUS       DASJUF010000131         6247 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJUF010000131 DASJUF010000000
VERSION     DASJUF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.133"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..606)
                     /locus_tag="U4B62_002532"
     CDS             complement(<1..606)
                     /locus_tag="U4B62_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol transferase"
                     /protein_id="HEN2142641.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISI"
     gene            complement(619..1617)
                     /locus_tag="U4B62_002533"
     CDS             complement(619..1617)
                     /locus_tag="U4B62_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142642.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            1815..3056
                     /gene="sstT"
                     /locus_tag="U4B62_002534"
     CDS             1815..3056
                     /gene="sstT"
                     /locus_tag="U4B62_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN2142643.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3203..3838
                     /locus_tag="U4B62_002535"
     CDS             3203..3838
                     /locus_tag="U4B62_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN2142644.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            4029..4769
                     /gene="truA"
                     /locus_tag="U4B62_002536"
     CDS             4029..4769
                     /gene="truA"
                     /locus_tag="U4B62_002536"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2142645.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(4914..>6247)
                     /locus_tag="U4B62_002537"
     CDS             complement(4914..>6247)
                     /locus_tag="U4B62_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142646.1"
                     /translation="TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRAS
                     LGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLF
                     LSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSD
                     MLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQ
                     LFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLS
                     GVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEIN
                     PTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIP
                     PGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2112 a          957 c         1184 g         1994 t
ORIGIN      
        1 gatactgata aatcgacgtt tccaatgaat cttcaaggaa tactgttttt gtagtctacg
       61 ttgtacaata atactagtaa tcaataatag gagaaggatc aatccgccca cgattaataa
      121 gactgggcta accatcgata atagttctga caatccggtc agcattttta aatctgaagg
      181 aaagacaggc tcttcccgca tttccatttt gattactgtc gaaatagtga gcagtaagtt
      241 aatgatcaag gtagcagtca aggcgtacca aaaacgattg gaaatcaaca atagtaagaa
      301 gaaaaatccc ataataatga tgattgtcag gatcatgggt gcttgtcgct gagtaaatat
      361 ttttaaggca acctggatag tttctttcca accttctttt ggttctaaaa cgaaaatctg
      421 cacgaaggta aagcagtaaa aaaatgctgc aactaaaaaa taacttttat gaacaaaaag
      481 ccaatgaatt ctttttttcc aacgctgagt taattgttca atcgaatcaa aagcgaagta
      541 ttcgatgaaa cgatgaatga aacggatttt ttgtatataa aataaaaaga taactaggag
      601 ggacactgct aataaatatc aaataaaaaa ctgcccgata tttatcagcc atgccttccc
      661 tgagtttgga aattcacgtt tcataaatgt agatacgcga taagaaacta aagggaaatt
      721 acatataact tgagtgacaa tcaaaaatac agcaagaaaa tttcctcttc cttttatacc
      781 taatttatca gatagcattt ctaaaaataa aaacgaagag aaagtataat acattgcgaa
      841 gagagaatat ggtttagaaa acctcagcgc tgtatctgta tttcctctga tgatgagtga
      901 aatttgtgtc atcaaaataa tcaaaccgaa taaaacaatc aagctgataa atacttgagg
      961 aagcttatag cggacctttt ctttccagtt ccattctttc aaaccataac ccattaaaaa
     1021 taaataaaaa atccataaaa tactctgtcc attctgtaat gcaaacgggt ctttattaaa
     1081 tagagtagag cagccaacta atccaattgt taaaaataat agtagatatt taattgttgt
     1141 tttcgataat ttatttaaaa atccaactgt ataaggaata ctacaaaaaa gcaaagtaca
     1201 agaaacagca taagtaaagt aattctgact gataggtgtt aggataagcc aataatcacg
     1261 aatattcaag ctgccagctt gtacgtaaaa aaagatattg caaactagtg tggctattag
     1321 gaatataaag gcaaaacgac ttaaataatg taaaactttt aaacgggcga ttttttgata
     1381 ttggtaaccc atgtaaagtg ggatcaaatt tgcaccaata acaacggaag tttgtaaaaa
     1441 tgtatagatg tagaatcgac cagggtttgt tgcatatgta ggaatagtga ctgctgaaat
     1501 caatatacgc tgtaaaacca tcattataca agcgacaaag attgtcagga agccataact
     1561 ctccttcttt atttttttca agaataaaca cctcacaaaa aaattggaca aaaacataag
     1621 aaaacgtttc ctgataagtt gcccattaat aacatcctaa cttagaatat ctcaaaaaac
     1681 aacgtgtaga cagaaaaagt acaaaattag caagaaatgg ttgtttgtat tgttagaatt
     1741 tttagaaaat tataccttta gtttctttgc ataaaatgat acaatgattg ttaaaattgt
     1801 gaaagggtgg tcatttgtta gagaagtttt tggatatgtc gttagttcaa cgtattgttg
     1861 ttggtatttt agtcggaata ttgtttggta tctttttccc cacatggaca tttatttcaa
     1921 tattgggaac gttgtttgtc agttgcttga aggcaatcgc acctattctg gtttttttct
     1981 tgacgatggc gtcaattgcc aagcataaaa ttgggaataa gacattcgtg aagcctattt
     2041 tgatcttata tttagtggga acattgcttt ctgcacttgt agcagttatt gtaagttcaa
     2101 tttttactgt tcctattact ttacaagaaa cagtatctga aaaagcaccg caaagtttgg
     2161 aaagtgtttt atctactatg ttgacgaatg tcacacaaaa tcctgttcaa tctcttattg
     2221 atgcgaatta ccttggcgtc ttattctggg ctgtattgtt gggactagct tttcgagcac
     2281 gttcagaatc gacaaaagag ttggtggatc agatatctat agcgttgtca caagtcgtac
     2341 aagtgattat tgcatttgct ccgataggga ttttaggatt ggtttatcaa tcaattgcta
     2401 cgactggaat cgctggattg gcagagtatc tgcagctttt acttgttttg atcggtacga
     2461 tgctgtttgt cgcattagtt gtgtacccat tcctcacgtt tttgtttatt aaagaaaatc
     2521 cctatccatt gattttcttt tgtttgaaag aaagcgcact tcccgctttc tttacccgaa
     2581 gttctgccgc aaatatcccg ataaacatga tgttggctga aagattgaaa ttgacaaaag
     2641 agtcttattc catatcgatc ccgttaggtg ctacgatcaa tatgggcggt gccgcgatta
     2701 ccatttcctt gatgacgttg actgctgtac attctttagg gattgaagca ccttttgttt
     2761 tgaaagtcct tttatgtatt ttgtctgctt tagctgcttg tggagcttca ggtgttgcgg
     2821 gcggttcact gttattgatt ccattagcat gtagtttgtt tggtatctct aatgatgtcg
     2881 cgatgcaaat cgtcggtatt ggatttatta tcggtgttat ccaggattca atggaaactg
     2941 ccttaaattc gtcaagtgat ctgttgttta ctgcaatagg agagctaggc gcgcgtaaaa
     3001 gaagtgggga aaaaatcaaa ttgaaagatt gtttagatgg attaagtgaa cgttgaacgt
     3061 tagatattta gtatttttaa aatgattcga gtttttaatg agttatatag atgttcgtgt
     3121 aaatagaggt ttctgttaaa tcaagataaa gtcgtttata ataacaatta aaagcatagg
     3181 taattgttat tggaggcgta tcatggattt tttacgtaaa tttggttttt tctttttatt
     3241 attaggcgtg ctgagtgatt ttttgactcc gtacgtatta ggattgtatt atcctgaatt
     3301 agatcaaatg aagagcgtga tcagtgtatt tggagaggtt tccagtcctg taagaaaagc
     3361 gtttcttatt tggtctgtgg tttcagggat ctttttcatc ctgtcattgc ctgcattgta
     3421 tgctgcattt gttagtacat ctcgtgtgtt agctacccta gttacagcag ccatcgcgtt
     3481 ctatggtata ggtgattgta tctttacagg tttgttcagt atcaataccc atgaatcttc
     3541 gtggaatgtc tcgacatggg tccataatat cggatcaggt ttaggttaca gtggtttttt
     3601 attgtttcca gttcttcttt tcttgatcta tcgaaaaaat gggcaaatgg acgtaagcca
     3661 cttatacctt ggactgtctg tgatcagttt gctttttgcg gcgatttatg gactggcaag
     3721 aattccttct ataaattctt ggccattact gaataaaata ggtttttgcc aaagaatcag
     3781 ttttttcttt aattatgtgc ccattgcctg gttgagtatc ttgcatctca aacagtaaaa
     3841 cttgagccgt caaattgaac agtcagcgga tacttcatcc ggtggctgtt ttttgttttt
     3901 ctgaaatata tatttccaca gttttcttgt atagcttctg tcttagagaa gcaaggagct
     3961 atattttgtg ctacaatctt aaaaaggtag agagaagacc aaatggaaga cagaggagcc
     4021 actaaaatat gagaaacatc aagttgacaa tcgaatatga cggaaaaaga tattcaggat
     4081 ggcaaagact tggagatgac gataaaacca ttcaaggaaa aatcgaaaaa gtattgcatc
     4141 agatgacaaa tgaagagata gaaattattg gatcaggaag aacagatgct ggcacccatg
     4201 cgcgaggaca agtagcaaat tttaaaacaa acacagaaat tggactggca gaaatgatcg
     4261 actttatgaa tcgttacctt ccgcgggata ttgtcatcaa aagaatcgaa gaaatgcctg
     4321 agcgcttcca ttcaagatat aatgcggtgg gcaaaaaata cagctattat gtttggaaca
     4381 acgtgattcc ttcagcattt gagcgaaacc acagctttta tttccctcaa gagttggata
     4441 tggacaagct gaatgcagct tgcgaaaaat tagtgggtac acatgatttc attggttttt
     4501 ctgcattgaa aaaatcaaaa aaatccacag tgcgtacgat tgatgaatta tctatcgaac
     4561 gaaagggaga aatgcttcat tttacttttg tcggagacgg attcttatat aaaatggtgc
     4621 ggattatcat ggggacatta ctagaaatcg gcacaggtgc catgccgata gaaacaatcg
     4681 atgaaatatt tgaaagcaaa gtgcggcaac atgcaggaga aaccgtccct gcacaaggtc
     4741 tgtttttgga tgaagtttat tatcgataaa aatactttga taatggatga aacgcatgtt
     4801 tcacagtata aatatcgatg ttaaataatg tatgaagcaa attaaagaaa tataaaaaaa
     4861 gactttttag cctgtttatg tgtacagcta aaaagtcttt cttaaataaa aatctagtga
     4921 gaatgttgta cttctcttct cggttgtttt tgtttagaaa aagtaaatcg ataaagtaaa
     4981 aataaaatca aagtaacgat agataaatat cttgaagcaa ttgctagttt tgtgtactga
     5041 taagtaataa cgactttatt tatacctgga ggaatagtta attcagttgt gccaaactta
     5101 gataattttg tttgtactag tttgccatta atagaagcca cttgcccttt ataacgataa
     5161 ataggtgtcg tcaagacagt atttttatta ttcgaattat ctaattcaat tgtaagtttg
     5221 ttactcataa aatgagttgt tggatttatt tcctggttac ctagtaaata ttgatcatta
     5281 ttaatcatat caggatgata gattgactct ttgtttgcat aatcagtatg attgtaattt
     5341 tcagctatag caacagcgtc ttcaggtgct ataagtgtta atgaatcttt gttagcatgg
     5401 atagaattgc ttactcctga gagatgcaat attagtgcaa gaaacgtaaa gaccattatt
     5461 ttccatgaat tatttttagt agaaaaaata taatagatca ctatactcat cgtataggct
     5521 ataaataaag aagaaaaacc atttaaccgc cagacaaatt gtagactctt aactggtgta
     5581 tggttaaaaa gttgccaagg aaataaattg gttgaacaaa aaagaacaaa tacaccaaga
     5641 aagaaaataa ataaatcatc agcagttaat ttttttataa aaataaaagc accaatcaat
     5701 ccgagaaata agaataatcc aatagtatag ttgttatagt tattatttaa aatatgtgtc
     5761 agcatatcac taggagccat accacttaat tcttttccca gtgggacttt caattcttgt
     5821 gctctagatt gttgaataaa aggaattaag aatcccgcac ttaaaaaaaa ggtgaggacg
     5881 gtagctttta aaagacttaa gagtctagcg attttttggt cccaaaaata aaaagaaaga
     5941 aacaacgttc ctccaataaa tacagaaacc atagcgacag aaagtaaatg agtatacact
     6001 actagcgtca tgccaataga caaccagtac catttttggt aatctctaat ataaatttcg
     6061 taacacccca ttagtatcaa aggcaaaaat gttaaagcga cagcttctcc caatgaagca
     6121 cgatggaata tgtcaatggc acgataggcg gaaaatgtat aaataatcga aaataacagc
     6181 gaaatataac gattgttttt tatttgtttc atagaaaaat aagaaacgac cattgttaaa
     6241 aaagtga
//
LOCUS       DASJUF010000132         6124 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.134, whole genome shotgun sequence.
ACCESSION   DASJUF010000132 DASJUF010000000
VERSION     DASJUF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.134"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..189
                     /locus_tag="U4B62_002538"
     CDS             <1..189
                     /locus_tag="U4B62_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2142647.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="U4B62_002539"
     CDS             complement(275..1369)
                     /locus_tag="U4B62_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2142648.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="U4B62_002540"
     CDS             1558..2319
                     /locus_tag="U4B62_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2142649.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="U4B62_002541"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="U4B62_002541"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2142650.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="U4B62_002542"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="U4B62_002542"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN2142651.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3959..4972)
                     /locus_tag="U4B62_002543"
     CDS             complement(3959..4972)
                     /locus_tag="U4B62_002543"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN2142652.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5137..6057
                     /locus_tag="U4B62_002544"
     CDS             5137..6057
                     /locus_tag="U4B62_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN2142653.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1880 a         1214 c         1094 g         1936 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     3961 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     4021 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     4081 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     4141 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     4201 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     4261 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     4321 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     4381 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     4441 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     4501 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     4561 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     4621 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     4681 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     4741 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     4801 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     4861 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     4921 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     4981 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     5041 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     5101 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     5161 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     5221 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     5281 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     5341 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     5401 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     5461 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     5521 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     5581 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     5641 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     5701 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     5761 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     5821 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     5881 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     5941 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     6001 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     6061 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     6121 tgtc
//
LOCUS       DASJUF010000133         6101 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.135, whole genome shotgun sequence.
ACCESSION   DASJUF010000133 DASJUF010000000
VERSION     DASJUF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.135"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(312..797)
                     /locus_tag="U4B62_002545"
     CDS             complement(312..797)
                     /locus_tag="U4B62_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142654.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(936..1151)
                     /locus_tag="U4B62_002546"
     CDS             complement(936..1151)
                     /locus_tag="U4B62_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN2142655.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1375..2175
                     /locus_tag="U4B62_002547"
     CDS             1375..2175
                     /locus_tag="U4B62_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2142656.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2194..4062
                     /locus_tag="U4B62_002548"
     CDS             2194..4062
                     /locus_tag="U4B62_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2142657.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4055..4546
                     /locus_tag="U4B62_002549"
     CDS             4055..4546
                     /locus_tag="U4B62_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN2142658.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4534..5088
                     /gene="srlA"
                     /locus_tag="U4B62_002550"
     CDS             4534..5088
                     /gene="srlA"
                     /locus_tag="U4B62_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN2142659.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5106..6101
                     /locus_tag="U4B62_002551"
     CDS             5106..6101
                     /locus_tag="U4B62_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN2142660.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2157 a          968 c         1222 g         1754 t
ORIGIN      
        1 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
       61 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
      121 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
      181 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
      241 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
      301 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
      361 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
      421 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
      481 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
      541 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
      601 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
      661 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
      721 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
      781 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
      841 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
      901 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
      961 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     1021 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     1081 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     1141 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     1201 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     1261 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     1321 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     1381 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     1441 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     1501 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     1561 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     1621 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     1681 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     1741 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     1801 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     1861 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     1921 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     1981 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     2041 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     2101 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     2161 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     2221 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     2281 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     2341 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     2401 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     2461 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     2521 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     2581 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     2641 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     2701 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     2761 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     2821 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     2881 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     2941 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     3001 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     3061 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     3121 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     3181 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     3241 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     3301 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     3361 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     3421 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     3481 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     3541 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     3601 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     3661 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     3721 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     3781 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     3841 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     3901 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     3961 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     4021 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     4081 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     4141 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     4201 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     4261 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     4321 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     4381 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     4441 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     4501 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     4561 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     4621 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     4681 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     4741 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     4801 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     4861 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     4921 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     4981 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     5041 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     5101 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     5161 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     5221 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     5281 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     5341 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     5401 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     5461 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     5521 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     5581 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     5641 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     5701 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     5761 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     5821 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     5881 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     5941 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     6001 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     6061 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DASJUF010000134         6097 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJUF010000134 DASJUF010000000
VERSION     DASJUF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.136"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            29..850
                     /locus_tag="U4B62_002552"
     CDS             29..850
                     /locus_tag="U4B62_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN2142661.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            870..1430
                     /locus_tag="U4B62_002553"
     CDS             870..1430
                     /locus_tag="U4B62_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HEN2142662.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2319..3086
                     /locus_tag="U4B62_002554"
     CDS             2319..3086
                     /locus_tag="U4B62_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEN2142663.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3112..3657
                     /locus_tag="U4B62_002555"
     CDS             3112..3657
                     /locus_tag="U4B62_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142664.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3658..4224
                     /locus_tag="U4B62_002556"
     CDS             3658..4224
                     /locus_tag="U4B62_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142665.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4239..4487
                     /locus_tag="U4B62_002557"
     CDS             4239..4487
                     /locus_tag="U4B62_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142666.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4506..4745
                     /locus_tag="U4B62_002558"
     CDS             4506..4745
                     /locus_tag="U4B62_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142667.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4762..5346
                     /locus_tag="U4B62_002559"
     CDS             4762..5346
                     /locus_tag="U4B62_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142668.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5453..5734
                     /locus_tag="U4B62_002560"
     CDS             5453..5734
                     /locus_tag="U4B62_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN2142669.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5724..6065
                     /locus_tag="U4B62_002561"
     CDS             5724..6065
                     /locus_tag="U4B62_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2142670.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2281 a          798 c         1269 g         1749 t
ORIGIN      
        1 aaaattaatt aaaaaggatg agattataat ggggttactg gttccgagag ctaatactta
       61 tactatacct ttaacgaaac gccaattaat tgctaaaaga tatcaaagga ttactagagc
      121 catcaataga gagttttgga attctgaaag tgatactgct cacagtttat acgtaggttc
      181 ttatgggaga ggaacggcaa ttagtactag tgatatagat ataatagttg aattaccgat
      241 ggcagaattt gacagattca aaaattattt atcaaatggc ccgtctaagt tattacaagt
      301 tataaaaaac gcatttcagg aaatactacc aaatagtgat attagagctg atggacaagt
      361 tgttaaaata aatttccatg atggaatcaa atttgaaata gttccagctt ttaatgaaaa
      421 agattattgg ggtgagtcaa aagggtttat ttatccagat tcaaacatgg gagggaactg
      481 gaaagctaca aatcccaaaa aagagcaaga agccatgaaa ttaaagaata caaaaagtaa
      541 taatttatta tatgctacgt gtaaacattt tcgacatgta cgagatacag aatttacgag
      601 ttatcatctt tcaggaatag taattgacag tttcgtatat gaggctatgg gaaattggaa
      661 attcgttgaa aataacagtg ggggtcaaaa cattagttct gtatcttatg agacagcttt
      721 attagaatac tataactcac ataaagttat gggcggttta aatttatact ctcctggaag
      781 caatcaattt gttaattctg atagtagtat catatgttta gaaaaagtat taaaaaaaat
      841 agctttataa agttttatgg aggaacaaca tgggagacga aaaaaagcgc ttggaagttc
      901 aattacgtga agcatatgga cgagtaacgt atacttacac cactcattta aagcaagccg
      961 ataatattat ttctgacaat aaaattttaa ggtgtcttca aattggtttg tcagccctta
     1021 ctactggagg ggtacttagt accattatta ctgataatag tgagttaacc tttgttgctg
     1081 gattatgttc aacaatttct cttggattaa acttatactt taagaatttt gatcttgctg
     1141 aagaagctaa aaatcatcaa attgttgccg ataaattatg gttgatcaga gaagaatacg
     1201 taagcttact aacagattta aatcgtttaa gtgttgaaga tattgtgaaa aaaagagatc
     1261 aattaatgaa cgaaactcat gaagcatata aaaatgcccc taaaacaaat agtaaaagct
     1321 atgaatcagc tcagaaagca ttaaaatctg aagaagagca attttttaca gaagaggaat
     1381 tggataagat gttaccagag catcttagaa aaaagagttc taaatattga actctttttt
     1441 tatgcttaaa attctcttgt aatttttcta ttcatctata tttaagaaga aatatgatga
     1501 tagaaacatc tcctaaagcc accgcaagcc gttctaagca tagcttagcc cgacattgct
     1561 agatatgctc atctcaccat gaacgtttcc taaagccacc gcaagccgtc ctagccattg
     1621 cctagtccga catcgctaga ttagtgcatc tcgccataaa agttttctaa agccaccgca
     1681 agccgttcta agcatagctt agcccgacat tgctagatat gctcatctca ccatgaacgt
     1741 ttcctaaagc caccgcaaac cgtcctagcc atcgcctagt ccgattttgc ttgatcggtt
     1801 ccatttctgt ttatttagtg ggtcaacacc gtaatcttaa atagccatcg cctatttaag
     1861 attactaggt ctgctttgaa ggtaaaaagt ctttcattct tcaagacttt taggctttca
     1921 agctacgcct gaaagcattg aaattcatcg ctctttattt ggttttgcca tttataattc
     1981 cttacgccca ttatcttttt cgcctgtcat accttgtcag aagtgtttag agcttatctc
     2041 atctttggat taatacaagt ggcgtaaatt tttttctaaa cgcaagcgtt acgaaaataa
     2101 tttacttaaa cacttgtatt aatccggcag ataagaacgc attctaatca ctcccgacaa
     2161 ggcaagacag gcgaaaaaga taaataaatg aacgaaagga agaaacataa aatgcaaaac
     2221 caaatagcga tgaagaaaaa accacctccc tctaaaacaa tccaaacaaa aaagaatttt
     2281 gaaaaaccaa aaatcaaaaa cagaaagaag cgttcaacat gagagataga attttagaag
     2341 ttaatcaaga aatggtaaac aagttaacaa atatcagtgg gaactatgaa aagtacgatg
     2401 gtactttact tgataactat atttttcacg atacacaaaa tatcagaatt ggcagaagta
     2461 aaccgagagc gttcattctc atcaaagaga aatatttgaa tgaatggaat agcacctatg
     2521 aaatggttat gactgataac tttgaaaagg tattggattt cagaaatgct ttgttgggtt
     2581 ctgatatggg aaatagcagt cttgaatcta ttgaagttga aaacaagacc gagaatgaag
     2641 gtgaaggatt gattaagaat gtcttgaaaa tcgaacaaaa atatatcagt gaagatagaa
     2701 gtttttatag agtcacaagc tcggttggtc tagcaaactt tgctactaac ttaattggaa
     2761 acaatgctag tgaatctttg ctagtgatgt gtctgaatac aaagaatgag gtcattgctt
     2821 atagcgaagt tttcaaaggt ggaattaatc aatcagtagc gataccaaga gagatttttc
     2881 aactagcttt gctaaacaat tcagcaaaaa tcgcagtatc acataatcat ccgagcggat
     2941 atgaatttcc aagtgaaaat gacaaaagtt ttactacgaa tttgaaaaac gcaggaaaaa
     3001 caattggtat tgaattgtta gaccatatca tcgtaacaag taaaaacaca agctattact
     3061 catttcaaga aaatggttct ttataaaaat tagagaaaag aggaaaacaa gatgggcgaa
     3121 agaacacaag cgttaattac agtcaaatat ggtaacagca aaacgaaagc aagcgtgtcg
     3181 attcattatc agtgggggta tggtcgatat atgctgatgg acgtattgaa tcaaggaatt
     3241 tatttaatca ataaagcata cgacttacga gataaatcaa gagaagaatt agcaaaagga
     3301 atctttgata gaagtacagg agttatcaat tttgtcacct atgaatctgg agtgagtgct
     3361 tatgatgaaa cagggtttgg tgggcgctat gaaccttatc aagaaacgat tgaagatgtg
     3421 atgaagtttt cagataacaa tgatggctat gctcatttag agatggtgtt cgatgaaaat
     3481 ggatttagtc tagatgataa aaccagattg cgattgtttg attttgaggg tcaacagata
     3541 ggactaaaca agttttgtga aagcggaaaa gagtttgcta cgaaagattt tattaaggga
     3601 tacagattat tgctagaaaa ttatggaata aacctagaag aagagagcgt gaaataagtg
     3661 tttgaaaaaa gaaaagacca gttccctaat ttagaagcga aaagtcaaaa agtgattgat
     3721 ggtgaaatga ttgaattatt ccaaaaaagt tatggaacca tagaaactac cgcaagttgc
     3781 aaatactact ttcaaggaaa gagaaacacg tttaaaggct ttgccgattc tgatgaagaa
     3841 ttggcaaaga aaatcattga ttggcttgtc agcagtgaac acgtagcaag agaagatacc
     3901 gttagaacaa cgattaatgg ggaagtccta acaggtacta gatgtgtttg ttgtaatcat
     3961 gaatttgtag aagatgaagg ttgctaccga atcaatgaat atggtgattt tatttgccag
     4021 agttgccacg agatagaatt tccaaccgaa agaagttggt ttgaatacat gctacaagat
     4081 tatgagatgg aaacagaaaa tggtgaagta gttttttgta ccaaagagga attgagtaag
     4141 agaagcgatg atgaaattga agattggtgt gtgtggttag tagggacaga tgattgcgtt
     4201 gcatattaca caacagcaga ataggagtgt gaatcagcat ggaaggaatg gattttatag
     4261 atcatgaaga tttgattgat tttggttata catggaaagg aatggtaggt attagcagaa
     4321 gtcttgctaa tgccttttac gaaagaaatt atgcagtcta tgtcctttat gatgacaata
     4381 cagaaagcct tgttgatgaa gagtataaat tggatttaga gaatgtttta tatgggatag
     4441 aaaaagaaga tttagcaaaa tatatttttt catggttggg tcaataaacg aaagagggta
     4501 gcaacatgac aaaattcaaa agtaaaggta tgcaatggtg ttcttattat tatgttggga
     4561 atgatgaaga gcttttgaga aaagccaaac aaaaaggtta tccagataag atgttaaaac
     4621 agattggcta ttacatgcca gaagagagaa tagaaaaaga atcttgtaag gttctttttg
     4681 atagtgaaca gctattagtt ttgttaactc aaaataaatt gatttgtcaa tttgaaaata
     4741 attaatatgg ggtgaagaac catgaatgag aataagatgg tattaagtga atacgcaaga
     4801 ttgagaacca ttaattggtt gaatggtatg caaagtagtg gcgcttttga tggatttgat
     4861 gaggagaata tggactttga aagtttatcg aatgaggata ttagatatta ttttatcgaa
     4921 tggtatttta cagaaccaga tgaagaggtg gaagatgaac cagaattaat tttagaacgt
     4981 caaagaattt tattcagtga acaagatagt aagaggttgt atggtttata tgacttacaa
     5041 ctgaaaggat taaaagcgca gtatgttgct gatagttacg atgatatggt agaacagggg
     5101 gcagataggg ttgtcactta ttcagaagat gatgaagagg aatgggaaaa ctattcagat
     5161 gatgagatat gctttaatga tgggtatatg cctattgaaa tcaccgaaga agaatatgat
     5221 aaagtgtcgg atagcgatga gattggtatg tgtacggttg taagtggatt ggatatagat
     5281 gtccaaagag tagaggttgc ataccagaag atgaaagaac gtataaaaag taaagtagaa
     5341 tcttgatttg attggattgt ttgcaaagta gctaattaag agtataatat aatcataaat
     5401 gtagttaatg gatataataa tgagtatatt atactcaaaa gaggtgaacg aaatgaaggt
     5461 aaaaaatcca aaagaaaaaa gcagaattca agtcggaata gacaaggatt taaaagaaaa
     5521 cgcagaaatg atattggaag agctagggtt aaatccaaca acagcaatca caatccttta
     5581 taagcaagtg gtcgctagag gtgagtttcc agtagagata aagctaagcg aagaggaaaa
     5641 gcaaggaatc agattgcaac agctaactat ggatatgcca gttgatgttc tagatacaga
     5701 tgaaaagtta gaagagtggt tcaatgaagc ctaatgagat agtaacggtc tatgttgcat
     5761 ttacagataa aaatggtggt aaaagaagac ctattttggt ggttagtgat aaagaagata
     5821 gagtagaatt ttttggaatc acaagtcaat atgagaataa atcagaccaa ataaagagag
     5881 tgtattttcc tattgcagaa tgggaaaaag taggtctaaa gaagcaatca tggattgacg
     5941 tagggtcttt aaaggctatt cccaaaagcc gagagaacat atcatttaaa aaggttggta
     6001 tgctatcagc tattgatacg aaaaagctaa ataggtttat cgagagatta caaagaaaag
     6061 tctaacaagg cttttctttt tttgtttata tgtatat
//
LOCUS       DASJUF010000135         6066 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.137, whole genome shotgun sequence.
ACCESSION   DASJUF010000135 DASJUF010000000
VERSION     DASJUF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.137"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(254..541)
                     /locus_tag="U4B62_002562"
     CDS             complement(254..541)
                     /locus_tag="U4B62_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2142671.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="U4B62_002563"
     CDS             complement(561..785)
                     /locus_tag="U4B62_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN2142672.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="U4B62_002564"
     CDS             complement(1240..1410)
                     /locus_tag="U4B62_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142673.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="U4B62_002565"
     CDS             complement(1425..1958)
                     /locus_tag="U4B62_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2142674.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="U4B62_002566"
     CDS             complement(1979..2923)
                     /locus_tag="U4B62_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEN2142675.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="U4B62_002567"
     CDS             3560..3646
                     /locus_tag="U4B62_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2142676.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="U4B62_002568"
     CDS             complement(3767..4456)
                     /locus_tag="U4B62_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142677.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="U4B62_002569"
     CDS             complement(4463..4741)
                     /locus_tag="U4B62_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142678.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="U4B62_002570"
     CDS             complement(4701..5615)
                     /locus_tag="U4B62_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2142679.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="U4B62_002571"
     CDS             complement(5597..5977)
                     /locus_tag="U4B62_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN2142680.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DASJUF010000136         6039 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJUF010000136 DASJUF010000000
VERSION     DASJUF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.138"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(67..1518)
                     /locus_tag="U4B62_002572"
     CDS             complement(67..1518)
                     /locus_tag="U4B62_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2142681.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1839..2603)
                     /locus_tag="U4B62_002573"
     CDS             complement(1839..2603)
                     /locus_tag="U4B62_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN2142682.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2631..3329)
                     /locus_tag="U4B62_002574"
     CDS             complement(2631..3329)
                     /locus_tag="U4B62_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN2142683.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3341..4120)
                     /locus_tag="U4B62_002575"
     CDS             complement(3341..4120)
                     /locus_tag="U4B62_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN2142684.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4136..5080)
                     /locus_tag="U4B62_002576"
     CDS             complement(4136..5080)
                     /locus_tag="U4B62_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2142685.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5126..5905)
                     /locus_tag="U4B62_002577"
     CDS             complement(5126..5905)
                     /locus_tag="U4B62_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142686.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1646 a         1260 c         1001 g         2132 t
ORIGIN      
        1 ctttatcaat ttttatccac tcattctacc actttagtaa tattttctat ttataacact
       61 ttcgtcctac attgatcccc ccctctgaag tacagttttt actgtcttaa ataaaatttt
      121 aaaatctaac cccatactcc aattttttat atattccgtg tcaagttttg ctacttcttc
      181 aaaatcagta atatcacttc taccacttac ctgccacatt ccagtaattc ctggcttaat
      241 agcaattctt gcacgatgat gataatcata ttcttcatac tctgatttaa ggcatggtcg
      301 aaatcctata aggctcatct ttcctgttag aacgttgaaa aattgaggga attcatctaa
      361 agacgtggat ctaataaatt gaccgattcc tgtctttttt gttccatctg gaagtatttt
      421 atttccgata attcttggat caaactccat cttaaacata agattattgc tcatcttatt
      481 ttctttcatt aattctactt ttctttcttc tgcatccata tacatacttc taaacttata
      541 catcttgaac ggtttaccat tctgacctat acgttcttgt gcaaagaaaa ttgggcctgg
      601 tgaattaata taaataagtg gagcaataaa gataaaaata attcccgttg caatacatcc
      661 aacaatacct gcaagaatat caattgttcg ttttataaat gcctgttgag ttgtcataac
      721 attgatactt gttgtaagta ctgtatattc tcctatacga ccaatagatt gttttcccaa
      781 agcactatct gatacttttg caagattcaa atgtacagta aggcccattt cagagcatcg
      841 ttcaataaat tctttcatac acgaagtact tttattcaaa ataataaaga cttcatctac
      901 ccatccctga cataagtaat ccgctgcatt tttggaatca gaaacaactg tcactccctc
      961 aacatcttca ccaatcatgc ttttatctat gataatgaca ccatttattt tatacatttt
     1021 atagttatgc tctttcacat tacgaataac tacttcagca atactactag atgtaactat
     1081 aataagtgat ttgttcccct catttttcat tttatgtttc aagtaatgtt tccataagag
     1141 gcgagttata tatgtaatta caacgtataa acctcccatc aagaaaagaa cgactctaga
     1201 ataatcattt ccaccctgaa cactaatgag atacaaactt cctaacagca ccaacataat
     1261 cgcttgcttt acagaagaag caaattctat atagtaccct ctttttaata cacctctata
     1321 tgcttcaaac atgaaaataa ctaaaagatc tgccagttca atgaagactg ccatgttttg
     1381 atacattgga atcacataag gattccaatc tacctggcgt aagacatacg atagataaaa
     1441 agcaagctgg atacacaata aatctaaaat aataaaatca atatgtttaa cccatcccct
     1501 tgaacttttc ttgtacattc ctttttactc ccctcttttt aataaaataa attgtattac
     1561 ttgttttctc tcctaaatat ctttctgaag gattattgac tcgacaaatt ggcttgtgga
     1621 tattcttata tatagaacat ccgtaagcca acttaaaact ccttaacaaa atttttttac
     1681 tcatattgta gatcaaaaca agtatccaag aaatcatatt ttatttcttt tttctgctta
     1741 ctcaacaaca gctaaaagaa aaatcatcat aaacaaattc aactaagcca acgatcccct
     1801 tgtgaaatca agttttttct tgatttacga ataaagagct aaaataaacc aaacttcttc
     1861 ttttttacct cttcatagac tggataactg atttcatccc cgttgatcag atcctttgcg
     1921 acttgtttca tctgtactac tttttcctta ccaaagtcct ttgcaatctg ctcatagcac
     1981 tcttttaagt aaaaggtgcg cttagagacc ccatgggcat cagaagcagc catctgcacg
     2041 agattatgtt tgaccatcgt tttggctgtt ttctggatgt ctttgccaaa taccccaaca
     2101 taacttggcg cagtgagttg ggccaaacat cccatatcta ggaacggaag caaatgattt
     2161 gggtcttcgc gaaaatgcac gttgcgttct ggatggacga ttactggtgt ctttcctagt
     2221 tctaacaact caaaaaaaag ccgttctgca tataacggca cttccagtgt cggaaattcg
     2281 atcagaagat atgtatcatc taaatctgta aataatattt catctcggtg gatgtcttcg
     2341 atgagcgttc cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt
     2401 ttctctaatt cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg
     2461 tattttccat tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct
     2521 ttttcagcca ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg
     2581 atatggcaat gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg
     2641 tagtaataac cagcatcttt gctatgttct gctccgttgt acacaacacc taagactcgt
     2701 gcttgtacca tctccagcag ttcttttgct ttagtcaagg attctttacg tgacatattt
     2761 tcacgtacga ccaataaagt gccatccgtt tttaaagaca tgatctgtgc atctgttacg
     2821 gctactactg gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcggatagg
     2881 atctgatcca ttcttggcga gcttaacaat tctgatgggt tgggtggctt aggtccacta
     2941 ggcaaaatcg acaagttctc gactggcgtt cgttggatca catctgctac acttcctgat
     3001 gaactaagtg ctgtacttaa tccactggca ttgttcagct ggaatgtctt atagacgacg
     3061 ggtttacgta aatctgcatc gactagtaga actcgttgac ctgatttagc aaaaacgact
     3121 gcaatgtttg ctgcagtggt ggatttccct tctccaggac ctgatgaagt cacgacaatc
     3181 gttttgattt gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat
     3241 tgttcggcga ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt
     3301 gctgcttttt gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc
     3361 tgcgtgatgg ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt
     3421 tacggatacc agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga
     3481 atcctaatgt atctgtcaca tatttttcat ctttcactgt ccgatcgagt aattccaata
     3541 agaatgccag acctacacct accatcattc ctaacattaa gccagtcatt aaattcaatt
     3601 tattatttgg cgaaacaggt gtcgtattgg caactgcacc tgaaatgatc gagattttat
     3661 caacattcat cacatctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct
     3721 gttgagcagc cactgcattc gtacttattg cctgaatcga gaacatttgt gaattttctg
     3781 cctgattaac tgagatggca tctttgattt gttcagcagt cattttcaca ttgtcttctg
     3841 tttctaaacg atctttgact tcattgagca ctaaatcact gacgatcata tctttatacg
     3901 tattgatcat ttgcaggttc gtattgacat cattgacatt tgcagtgtct gattgtggca
     3961 gtgtcacgat caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga
     4021 ttcctgagat ggccaagcca gcaaacattg agatgatgat tattaccaag tgtttgcgta
     4081 atgtttcaaa aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt
     4141 tattctctag attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcac
     4201 tgactttttg ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct
     4261 taatcgtacc aaatgcctcc cgataatcta aagcaatttt tttcatttca tcaaagttca
     4321 tgtctgtttt catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt
     4381 ttagactcaa tacttttttc gctacgcctt ctacgatctt tctttggcgc ccttgtctac
     4441 cgtaatcacc atttgggtct tcataacgca tacgagaata agataatgcc tgatccccgt
     4501 tcaatgtaat tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat
     4561 tgacttcgat accaccaact gcatcaacga gttcctttag tcctttcata ttgatagaga
     4621 catagtgatc gattgggatg tctacatatt tttgtacggt atccatcgcc atggcagctc
     4681 cgccgaatgc gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat
     4741 acgtatctcg aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa
     4801 ccatcatcgt atctgatctt ccttgatcga cccgacctaa atctccagta tcaatcccca
     4861 tcaatagaac agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt
     4921 gggttcgttc cactgattcg taagttttct caatagaatg tgagagatcc agatataatt
     4981 ttgctcctac tcctacaatt gctaatacta atacgataag aactgctaaa ataatcaaaa
     5041 atattttttt ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac
     5101 cttgcttgaa tcaacgtttt aactgtcaat agcttatatt gctatagccc atttgctgag
     5161 caacagtttg tgcttttgtt gcgtatgcgg ctcttatttt ttcattccgg atctgctcta
     5221 tttgactgag cgccgtcaaa tattgatcgt aagtagcggt agctgttact tgaccgtctt
     5281 tcagcatcgt atcgaatagc tgatctagtt gcgtgacttg tgcaatttgg gtatcagcga
     5341 atcctagata ttgcataacg actgttttcc aagcactatc cttaaaaaag ctcatatttt
     5401 cacgtacatg ggcgacatct gcacttgcca acttttcttt gatgatgaca tcatccacag
     5461 cttgctgcca gtttgatacg tttttagtaa ataatttatt gacagccgtc tggatcttca
     5521 gttttgattc tgcatcctct aactgcgtta agactgcttt cttttcttct tggatcgcag
     5581 cagcttcttt aggtaattct gattccttga tgtcataatc atctgccgaa agtttgatag
     5641 aagatagttt atgtgacaaa tttgaaactg tttcttctgt tatttcagga tttaggaaaa
     5701 ttttttcttt tgtactgtac agcttatcta cttcttttga taaagaggtc acggtgtctt
     5761 tttgattggt gatggcagat tctgcgtaat tgatccgttg atgatattgc cgctgattat
     5821 tgagatggta tccaaacaag caaatcacaa tagcgcctgt gcctaccgcc acagtagcca
     5881 ataacttgac gaacggcttt tccatagatt cactcctaaa tattgatgat agataagtaa
     5941 agatgtgatt cattcacatc agaatgagcg ctttttattt taaaagcata attaaactaa
     6001 tatattcata gaaatattta taaacaggtt ttccctata
//
LOCUS       DASJUF010000137         6021 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJUF010000137 DASJUF010000000
VERSION     DASJUF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.139"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            23..1033
                     /locus_tag="U4B62_002578"
     CDS             23..1033
                     /locus_tag="U4B62_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2142687.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1102..2046)
                     /locus_tag="U4B62_002579"
     CDS             complement(1102..2046)
                     /locus_tag="U4B62_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2142688.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2030..4750)
                     /locus_tag="U4B62_002580"
     CDS             complement(2030..4750)
                     /locus_tag="U4B62_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2142689.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4747..5967)
                     /locus_tag="U4B62_002581"
     CDS             complement(4747..5967)
                     /locus_tag="U4B62_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN2142690.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
BASE COUNT         1740 a         1171 c         1001 g         2109 t
ORIGIN      
        1 aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc
       61 gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat
      121 gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa
      181 ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa
      241 agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac
      301 ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag aatatattcg
      361 taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt
      421 gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag
      481 tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt
      541 agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga
      601 ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg
      661 tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt
      721 tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact
      781 aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg
      841 tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag
      901 tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa
      961 gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga
     1021 ttcatcaaaa taaaagaaaa caaaaaaagc gaagacttat gtctcgcttt ttttgttttg
     1081 aatgaaaaaa ataaattttt gctataaatc ttttggtaca tagaaacttc ggttgtccat
     1141 accgaaaatc cgatcggtgt attttcctgg ttctgtttga cgaatcgaag tcaacatcat
     1201 ctgcatcgaa gcatcgatat tgtcgatttg atgtaatatt tctgcttcca ttattcttgg
     1261 acgtacaggt gagccatatt ctaataagcc atggtgtgct aaaaccatat gacgtaatac
     1321 gagaacatct tcgtcagctt cgctgatttt caacgcaaga caggctttag ttatttcttc
     1381 gtcaatcaat accagatgac cgatcaaatt tcctgcaagc gtatattctg tcgacatagg
     1441 accggaaagt tcaatcacct ttcctaaatc atgcaaaatg ataccagcat acaacaatgc
     1501 tcgattcagc tcaggatatt catcggcgat tgatttccct aagtgaagca ttgtcaaagt
     1561 atgataagct aatccactgg caaatgcatg atggtttctt tttgctgccg gaaactcaaa
     1621 aaattgtttt tgatatttat taagtagata gcgaacaata cgattccaat gcgcattggt
     1681 gatctcaaac aacaattgat tgatttcttc ttccatttct tctttcttca aaggggctct
     1741 ttccatgtat aaactaggct ctcctggctc atctaccctt gttaagcgca tatgaagaat
     1801 ctttacttga gggtttcctt gataaacctc tcttttaccg tttagaaaaa cgactgtgcc
     1861 ggctgtaaat ttattgattt cctcctccga agcatcccag tatttaccat cgatagtacc
     1921 tgaagtatct tggaaagtaa aagcaataaa tttttttccg tttttagcga ttcttacatc
     1981 tgcacttttg atcaggacaa attgttcaaa ctgctcatcg attactaaat cacgaatttt
     2041 tttcattatt tgctccttct aattgtttta cgatttggtt catctcatgg taatagctta
     2101 ccatttcttt atcagaagaa aaacaaatta tttgatactt ttctccaaat ttagctaaaa
     2161 gttcagctaa ttgatacttt cgctgatgat cataatgaag ccagccatca tcaatgatga
     2221 tcgggcatat cgaacggtta gcttctaatg agaggtaagc aaatctcatc gccataatca
     2281 gctgatcttt cgtacctgta gataactcat agatgggaaa ttctgtatat ccgtctgtta
     2341 aagtcagtgt atcatttttc agtaatatct tctgataatt tccttttgtt aaaataccta
     2401 aataatcacc tgctgctctt aacaactggg gtaattgtct ttcactcatc tctgttgaaa
     2461 gatcacctaa gaatgtcccg gcgatttcaa gtgctcccca ttcctccagt aattcctgta
     2521 attttgcttt ttgccggcta gcttgttgat aaagttcgtc caatgtgccg tctctttgca
     2581 actcttctac ttgaagttgg agtcgttgcc gattttcact tgcttctttt tcttcttgct
     2641 ggagttggga ttgcttttct ttcagttggc gcagtcttac agataatttc tcttttgtca
     2701 gctcttcagg aaatagtgga gtaagcattt ccgataattc attgtatctt ttcatctgct
     2761 gttcggcttg tttggctttt tgtaaaaaga caggtacttc cgaaggataa gataagcctg
     2821 cttgagtcaa ttgttcgctg tattcttgta tccatacttt ttgtttcttt ttcaattgat
     2881 tgatttcttg cttcaataaa gtattttgct gatagcttct ggcaaatttc atctgttcca
     2941 tctcttgtat aaaacgggaa agcaatacca ttttttctga aattgtctta tcttgtatcg
     3001 gtaaccattc tttaacaaac tcaaattttt ggtcgatttg tcgtagtttt tcttgtattt
     3061 cctgttctct tttatgtttc cgctcaaata cgccatataa tttctgataa tctatgactt
     3121 cctgtccata ttctttcatg agtttgagcg tatccatctt tcctaagtgc cgatgatccg
     3181 caaaacgata tacttcttgc tgaagaatct gtttttcttc tccagttctc ttttgttttt
     3241 cttctgcttc agctaattga gctaaggcta gatcaagttg tcctagtttt tcttgccaca
     3301 ttccttttat ctcagagaaa tcatttttag ttttaatttt tggttgcctt ttgtagaaga
     3361 aatagccaat catgcctgca ccaattagta aaaggatcac tttcaagggc attggtacaa
     3421 atatacctgc aatgattatt gctccgccag ccatcagcat atacggttta gatgaagaga
     3481 ctggtgctat ctctgactga ttttgtaata atgctggata ggtctcttcc aagtcattca
     3541 cttctgcttc aatcatctct tgctgttctt tagctatttg gagacgcaca gaaatctggc
     3601 tttctagatc agcaatcctt ctttcttcag ccagaagatg cttccattct tcttcatata
     3661 aaaattcttc cggtaacgga tttttctgcc aatgccacct ttgttccaaa atcttcaatt
     3721 ctgaatttgt ttctctagtc tcaatttgta atctttgcca ttcttcgttt aattgttccg
     3781 ctgtaaattg aagttgctgg agatctttta gttcctgctc ctgctctaaa taaaaataat
     3841 atctggctga ctgctgatcc atcccgctat gcttttccag tgttgcttct aattcttcca
     3901 aacgctcatt tgtttgctga taccgtttgg agaaagactc cagatctaaa agaagttgct
     3961 catcaggaag acctgtagtg ctgttttttt tgagagattg ccattcttca tataaagaaa
     4021 aattaagttc ttgctgtctg gctaatgaaa attgtttgtt cgcctctttt aattgtgcca
     4081 ataactgctg ctgttgcgac tcgtattcct gttcttgttg gatcaacttg ccaaaataag
     4141 cttcctgttc ttgtttttga tgaatttttt cttgcagctc tttccattca tttaattttt
     4201 gattgattag taatttttgg ccttttttct tgaataattg ttgtgctttc tgataatatt
     4261 cttgccgctt ttggaatact tgagaactgc ccgtgatgcc taaagaaatc agcgcatcat
     4321 gtagctcttt ttctctcaaa tgatctagtt ccatcaactg ctcttgctgg aaagtaaata
     4381 cctgctggaa taactctttc gtcaaaggtg cgatcagttt ttctagtaac tcatcacttc
     4441 ccacttgatc ccccagccac accttcgatt ttccgcgatt ttgctgcttg tagcgctcga
     4501 cagcaaattc accatataca ggatgtttaa gccatatttt gccgccatag gctgagccgt
     4561 cttttggtgt ataatccttt tttttacggc cttttgaagg gaaaccaaat aatattgctt
     4621 gaataaattg ataaatcgtt gattttcctg cttcgtttcc cccatagatc agctgattgc
     4681 ctgaataaaa gtcaaaactt ttttgacgcc attttccaaa agcagcaatc tcgatcctta
     4741 aaatcttcat tcctgatcct cctcaaataa aaaatcttga ttaagcagct gcttggcttt
     4801 ttcaagtgtt tcttcttgat attccggcag ttcgctcaat attctagccg cttcttgatg
     4861 actatatact tcatttaaaa tttgttggaa atcttgaggt gtctgatagt attgaaataa
     4921 ctgttccatc aatttagcag aagcagcaag tggggtcttc gtataaaaag tattctctat
     4981 gagagaaata cgccaaataa agaaatcatt tatcctatct gagaattcct ctaacaaata
     5041 gtctaatagc tctcctgatt gtatccgttc caaaacatct gtattgagat ggtcataatc
     5101 ttttaatttt atctcaatta aactaaatcc atcctctact tttgaaagct gctgacgaat
     5161 gtgaacaatg acctcttttg tcgttcttgc tttcctcaat gatatttcct ttgtcttcca
     5221 atagacctct gctactttaa tcggatgtat ttggcaactt cctttactta attcaactag
     5281 tagaatagat gtagaagctt gttccttttt agtatgacct tgaggcgcac ctggataaac
     5341 aattgtttgc ttttcattta agaccgttgg cacatggata tgccctaaag cccaataatc
     5401 gtatcccttt tcttgcatct ttgagggttg gaaaggagca tagtttcctt tttgagagac
     5461 gccaggttcc ccgtggtaca acccaatatg gtaatctgtg agttctctag aaggaaattc
     5521 catcactttg tctttttgaa tccacggttg acgataacta aatccactga gagagacagt
     5581 ttctccactt tttatcgtta ttttttttgt ctctaccgtt tcggatgtaa ataaatggac
     5641 atttttagga aaggagaacc aataccgctc tttttgatag aaatcatgat ttccgaatat
     5701 tataaataca ggtatatggt tcttttctaa ccgtttcatc tgttctgaaa aatgtttctg
     5761 tatttttaat gaaggacgat tttgatgaaa attatctcct gcaagtaaaa caaaatcaac
     5821 gttatttatt attgcctgtt caatgatatt tgataaaacg gtcagattcg tcttcaatag
     5881 tttttcctgt acagttttat caagagttgt cagtccttca aatgaccgat ccatatgtaa
     5941 atctgccgca tggataaatt tcaccattaa aaatttcctc ctttttttct tttatcatac
     6001 cattttcaat gttttttttc a
//
LOCUS       DASJUF010000138         5896 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJUF010000138 DASJUF010000000
VERSION     DASJUF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.141"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            138..1292
                     /locus_tag="U4B62_002582"
     CDS             138..1292
                     /locus_tag="U4B62_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN2142691.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1286..1708
                     /locus_tag="U4B62_002583"
     CDS             1286..1708
                     /locus_tag="U4B62_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142692.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2842..3810
                     /locus_tag="U4B62_002584"
     CDS             2842..3810
                     /locus_tag="U4B62_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2142693.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3820..4308
                     /locus_tag="U4B62_002585"
     CDS             3820..4308
                     /locus_tag="U4B62_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2142694.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4604..4885
                     /locus_tag="U4B62_002586"
     CDS             4604..4885
                     /locus_tag="U4B62_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142695.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4887..5303
                     /locus_tag="U4B62_002587"
     CDS             4887..5303
                     /locus_tag="U4B62_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142696.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2187 a         1016 c          966 g         1727 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatttaaca caaaggcgtg attattttga aacccaaaaa gaaaatttat ctaggtctcc
      181 tcttttcctt gatgattgga atttttagta taggaattac aaatacctat gcttcagaaa
      241 attctaattt caatatcaac tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa
      301 ctgattttga acactccata acttcaattc ttttaatgga aaataacacc gaaactgata
      361 ttaactatac tcttggttcg ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt
      421 ctattttctt tccagtattt tcagacgctg ataatacgat taaatatgtt tataccgtca
      481 aaaaaaatac aaatggagaa tattcaggaa gtatttcaca atttttagca aaagaacttc
      541 aaaatctagt agatagtaat ataacagata caaccttatt ttttcaagat aatagcattt
      601 attattccga taatggacaa attaaactac tttggaaatc acctttaagt acagaaaaaa
      661 atggtgtttc ttcccttaca gaaaaaaatg atcttatatc cgtaaatata aaaaataaaa
      721 ttaaaaattt ttctaaaaaa caaaattttc ctgcggttaa acgtgtaaaa agtgtctcct
      781 ccggagataa ctttattatg attaattggc gtatagcaga aacccaaaca agttttccgt
      841 ggtgcgctgc ttacgtagaa gctgctatct tatgtaacaa aacagatgta cgtgctactt
      901 cagctagagc cattatgcat tatacctatc ctaatttaaa caacagtcaa attgaatcaa
      961 aaagtcttaa tcaagatcaa ataataaaat atgcaaatta ctgtggtgtt tatcctaaaa
     1021 aaatagcacg aatactaaac ctttcagagg tccaaacaca actaagaaaa ggaaatgcca
     1081 tttatatggg aactgctggt tatggaaatt atagtaacag ccgtcatgca cttgcattat
     1141 ttgggtgggt caatcaaggt ggttatcaaa cttactatgt gtggaatcct tggtataact
     1201 acccaactgt aataagtgca aatgcaggac cagttacaat tcctgtacct ggaggtggtt
     1261 ttacttggga caataccatt acaaattggt aaataaactc attgctttag ttatgggtct
     1321 agtgttagtt cttttcttaa ctagttgttc aaaaaaacaa gaaatacaaa agactgatct
     1381 gattcctttt ctaggaactt ggcaaactaa agatcattca gaacgtctag taatcactaa
     1441 tttaaatact tcaaaaaata tagaacaata taccatctta gtaaaagaga acaatcataa
     1501 aattcaaatc attgataaaa atttaaatat aaatgaacaa gttttcattt acaaagcaac
     1561 aaataattta ctaacactgc gctcgaataa aaaacaatta gattacactt ttagtttgga
     1621 ttctccaaaa aagctcgtat ttattttgac aacgaatgat gggacagaag ggacagctaa
     1681 accaattgaa ttttttaaag ctgattgatg aatagttgtt tgatattaca aaacaataat
     1741 ttcaccgaag gtgaaacaag cgaaacttta caaaaagtgg ctagcatttg ctagcctttt
     1801 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     1861 agcggttatt tgcaccaaat acgtaggtat ttggctagca aatatagagc ttgccaagcc
     1921 ctttagtgtg ttttgtagaa agggacctta tagtcacttt cacattacaa acgatacgtc
     1981 ctatggacgt atcaaaaggg tgggacttat gagcaaatta aagcaaaatt tggctagcaa
     2041 tccttctaaa aaatacacct attgatcaga gtcaaaataa atcagttttc gagtgagcga
     2101 atccaaattt gcaactctga agctttgcaa atgagtgtgc cggcttccgt aaaagtctag
     2161 gtaacattag tcgatatata catagggctc taatttgcgt tttaaggcgt tttaaacgtt
     2221 ctggtatgta attataccaa agcgacttta aaacgcctta aaacgaattt tagaggttct
     2281 gtagaacgtt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaaaaaca
     2341 agataaggga ttttatctag acttgtctta aaacgccgta gatgagcgct cagagcttct
     2401 aaaaggactc cttacgaact gtttttaagc aaaggagcct ttaggcgact gcgcagttta
     2461 atgtgagagc gtagcgatca cacctttaaa gcgaccgtag ggagcagatt cccagtttag
     2521 gaaatttcgc gaacctgttt ggagaaaaaa agtaggaaaa ttttaggcgg gaaattttgc
     2581 ccccctcttt tataatctct tttataacct ctttttaaac ctctttaccg tcggctgtat
     2641 ccttactctc ccaaggtgtg cagaatcttt taggggtctt tttgtctgtt tttaggggtc
     2701 tttttgtctg tttttagggg tctttttgtc tgtttttagg ggtctttttg tctgttttta
     2761 ggggtcttta attagggggt tgacccccat tagaaagctc tttataattt tgttaagtag
     2821 ttaatcttga aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat
     2881 acgattcctt tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tataatatct
     2941 agaatgcgtg atgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta
     3001 agcaattata aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac
     3061 aaattaatgg atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt
     3121 gttttattta atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa
     3181 gtccatgaga aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc
     3241 caacaattca atgaattaga aagcagttat tccaaaacca tgtttagatt attaaaacaa
     3301 tacagaacca aaggaattgc caatttttca aaagaagact ttcatgaatt acttgatatc
     3361 cctaaaagtt atagagaaag tgatataaat aaaaaaatta taaagcctat tcgccaagaa
     3421 ctaaccgcaa tcttcaaagg tttgacaatc caaaagagat acggaaaagg acggggaaag
     3481 cctgtcattg gctatcaatt tacatttaaa cctgagataa aaaaatcaga cgactttaac
     3541 aaacatgctc caaccaaaga agaacaaaat aaagctttgg aactagctaa tcaacaggtt
     3601 caatctgaaa aagaacctac ttcattacaa gaaaaaatca aagaacaaaa acgaaaatta
     3661 gaacttttac aacagttagc agaattagaa agaaaacaaa ataccccaag taagaaaatt
     3721 aacaaacaaa tagcagataa caattccgat attaccccag aatttaaaga aaacttacta
     3781 gataatctcc aaaacttatt taaagaataa ggggttttta tgacagataa aactatcaaa
     3841 gaattagcag atattctagg tgtttcaaaa gataaagtga agtatcaact gaggaaatta
     3901 cctagtaatt ttacctacaa aaaaggaaat attacctacc tgaaaaaaga agcagtaaca
     3961 acaattacaa aggttttatc aggtaatgaa tcgggtaact tacctagtga attacccgat
     4021 tcattaccta ccgattacct actcaaggag attgaaacta agaataatca tattgaagaa
     4081 aaagataggc aaattgaaaa acttcaaata gctttagaaa cgcagcaaaa attactcgac
     4141 cagcaacaac aactcaccct acaagccaat caacaaatca aaaaacttca cgaacaactc
     4201 cagctaacct atgcagaaga atcttcagaa aaagaacccc tttctgttac tgaagaaact
     4261 caaaaaggac aaaacaagga gaaaaaatgg tggcaattct ggcaataacc caacaatttt
     4321 tattcataaa aattgttggg ttactttctt tttttgcttt tacaagcata gcgacatgat
     4381 ctcacgtcgc tattttttaa agagcataag gaaacgcggt atcactttct tacgaaaggt
     4441 gctaggtagg ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgtca
     4501 ttgtggatag cttatgtata gtgtttcggc aaaaaacagc tatacaaata ctatatttgt
     4561 atgatataca taaattatac acaaatgcga aaggaattct tctatggaca ttttagttcg
     4621 gaatattgat ccaaaagtta tttcagaaat agatagacgt tgccaagaac tcacaacaaa
     4681 aactggcaaa aaatggagcc gtaatgcgta cctaaaagtt ctaatcgaaa acgattttga
     4741 ccaggcactt aggcattaca aaaaagatca attcgattta ttactcgaaa aatttatcga
     4801 tatacaagca aaaaatactg aaacattgaa cgactatgtt catacaaaca accaactcat
     4861 tgaaacactt atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc
     4921 tttatcccag tcacattgac gttttagatt ccatcatcga aaaaaatcca gatatcaata
     4981 actactcgga agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa
     5041 ctaccagcaa ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa
     5101 tcattgcaga atttgctgat ttatatctaa aagatactgg tgttcttatc ggtacaaatt
     5161 ctaaagtata tcaagaagtg aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa
     5221 atcaacccaa aaatcgtcaa aaagaaacca atcaaacatt cacttctaga aattttgacc
     5281 caacaaactt tctaaaaaga tgaaaaagaa accgaaaatt ttttctattt ctcagtttct
     5341 tttttagggg gcagtcttcc agcaagattt gcaagttacc ggtaacttgc acaaaaaaag
     5401 aaaaatccgc ttgtgcgaat ccttcttttt gcattttggg gcgctgcccc aatctccaaa
     5461 tatcggttat ttaatagcgt ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa
     5521 gactgctcta acgagcagcc aataaaaaat aatgagcgtt tattcttgaa aaagcagcgc
     5581 gcttatgaag cgctgccaaa aatttaccat attttatgaa aaataacaag tttctaaaaa
     5641 ggaatcggtt aagattcaca aagaatcgta aacagacaat agcggaaaat caagcttccg
     5701 aacgtacctt ttagctagac caatgaaaaa tcacaaagtg agcccctgta tcggttgata
     5761 caagggctac ttggcatact cgacattatg tctactagct cgataaataa cgattatgtt
     5821 cccaaaacaa tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa
     5881 ttataggtgt atatca
//
LOCUS       DASJUF010000139         5852 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.142, whole genome shotgun sequence.
ACCESSION   DASJUF010000139 DASJUF010000000
VERSION     DASJUF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.142"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            95..1726
                     /locus_tag="U4B62_002588"
     CDS             95..1726
                     /locus_tag="U4B62_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN2142697.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1729..2808
                     /gene="uxuA"
                     /locus_tag="U4B62_002589"
     CDS             1729..2808
                     /gene="uxuA"
                     /locus_tag="U4B62_002589"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2142698.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2882..4309
                     /gene="uxaC"
                     /locus_tag="U4B62_002590"
     CDS             2882..4309
                     /gene="uxaC"
                     /locus_tag="U4B62_002590"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2142699.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4393..5040
                     /locus_tag="U4B62_002591"
     CDS             4393..5040
                     /locus_tag="U4B62_002591"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEN2142700.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5160..>5852
                     /locus_tag="U4B62_002592"
     CDS             5160..>5852
                     /locus_tag="U4B62_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2142701.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSL"
BASE COUNT         2000 a          798 c         1221 g         1833 t
ORIGIN      
        1 caagtttatt ccctttttat attgacaacg gttacaatgg gttttaaaat ataaaatgta
       61 tcaaaactaa catatcagtt tgaggaggtt aattatggtt tcgattaaaa attggcaaaa
      121 ttctatcgaa gaattcaatg aaatgcagat tctactgccg tgttttgatg tcaaagaagt
      181 taagcaagaa ggccagcaac atccagtatg gcttcatttt ggtggaggaa atctttatcg
      241 aggatttcat gcagaaattg ctcaatgctt agcagatcaa aatgaattag aaacaggagt
      301 agttgtgtgt gaaacatttg atgaagaagt agtggaaaaa gcttatcatg actataacaa
      361 tgacattttg gaaattgtta tgagtggaga tggtgaactc aaaaaaagac ttttggcagt
      421 aacggctgat agtttatatt gcaatccgaa gaatatagcg gactatcaaa gagtggttaa
      481 atattttgaa gatcctaaat tacagtttac aacgtttact attactgaaa aaggttacag
      541 tttaaaagat tctaaagggc aattcttttc ggtagtcaaa gctgatatca aatctggacc
      601 tagcaatcct caacatacga tgagtattgt aacatccttg ttatatagca gatttaatgc
      661 tggagagttg ccgattgcga tggtttctac tgataatttc tcacagaatg gtcaacgttt
      721 tcaagatagt atttttacga ttgcagaagc ttggcaaaaa gaaggatatg tttctgaaaa
      781 atttattgct tatttaaagg ataaagaaaa agtgactttt ccttggagta tgattgatcg
      841 aattacgcca aatccttcag agaatgtaaa ggaagatcta ttgatgatgg ggctcgagga
      901 tttagatatc attcacacaa aaaaacatac caatattgct gctttcacta atactgaggt
      961 agttcattat ttagttattg aagatagttt tccaaatggc cggcctaaat tggaaaaggc
     1021 tggtgtgatt atgacagata gagagacggt tgataaagca gacatcatga aggttacgac
     1081 aagcttgaat ccacttcata ctgctttggc agtaacaggt tgtctgctag gatatcaatc
     1141 aatttcagca gagatgcagg atgatgattt ggttggttta atcaaaggaa taggttattt
     1201 agaaggttta ccagtagttg aaaatccagg cattattgat ccaaaacaat ttattgatga
     1261 agtaattaat aaacgtttgc ctaatcccta tattccagat acaccacaac gaattgcaac
     1321 agatacttct caaaaagtag cgattcgcta tggtgaaaca attaaaaagt atgtcgaaaa
     1381 agacggaaat gcagaaaatc taacatttat tcctatcgct attgctgctt ggttacgtta
     1441 tttactggca gtcgatgatc aaggggaaaa ttttgtgcca agtcctgatc cgttattggt
     1501 agagttgcag gataaattat cagtattgaa attaggtgaa caagatcctt caaagattca
     1561 ttgtgcggta caggaaattt tagaaaataa agaaatcttt ggtttagatt tatatcaagt
     1621 tggggtagga aagaaaactg aaaaaatctt ttgcgaaatg ctagtaggtc ctcaagcagt
     1681 tcgtaaaaca atcaataaat atataacaga agttggagga aagtaactat ggaaatgact
     1741 tttagatggt atggggcaaa tgaagaaaaa atctcactac aagaaattcg tcagatccca
     1801 gcaatgaaag gaattgttgg aacattaatg gatataccag ccggagaggt ttggccgaga
     1861 gaacgaatta aatctttaaa agatgaagtc gaagccaatg gacttacctt gaaagttatt
     1921 gagagtgtta atgttcatga tgatatcaaa attggtttac caacacgaga tcaatacatt
     1981 gagaattata aacagacgat tcgcaatttg gcagaatttg ggattgaggt tatctgttat
     2041 aattttatgc cagtatttga ttgggtaaaa tcggatttgg attatcgttt agatgatggg
     2101 tcttctacgt tagcctatat ttcaaaaaat atccctactg atccggagga aattattaga
     2161 gaagtagaaa attctactaa taattttgaa ttgccgggtt gggaacctca tcggttatca
     2221 caaattaagc aattattaga agcctataaa gaagttgatg aagagaaatt acgagagaac
     2281 tttgcatatt ttctcaaagc aattatcccg acatgtgaag aagttgggat taaaatggca
     2341 gttcacccag atgatccacc atattccatt ttcggattac ctcgtattgt taaaaatcgt
     2401 gacgatttag attggttgtg taatgtagtg gattctcctt ctaatggtat tacattatgt
     2461 actggaagta ttgcagaacg accagataat gatgtctatg aaattctatc tgaattttgt
     2521 caacgggatc ggattccttt tgcacatgtt cgtaatatca aatatttaag tgacaatgga
     2581 ctcgattttt acgaagcgcc gcataaatca gaatatggtt ctctagatat gtataagata
     2641 ttaaaaacaa tgtacgataa tggatttaat ggctatattc gtccagatca tggtcggatg
     2701 atttggggag aaaccggtag acctggttat ggattatatg accgagctct aggtgcagtt
     2761 tatttaaatg gtatttggga agcgcttgaa aagggggcga gtaaataatt atataaggga
     2821 gtttatttag aggaaaaatt ttctcttcta aaaactaacg gaaggaaagg agtgtttaaa
     2881 tatgttttta gataagaact ttttattaga gaatgaaata gctaaaagac tttttcataa
     2941 atttgcaaaa caccaaccaa ttatcgacta tcattgtcat ttagacccag catatttgct
     3001 agaaaataag caatacaaga atttgactca ggtatggtta aatgataaag gagctggaga
     3061 tcattataaa tggcgattga tgcgagctaa tggtatagaa gaagagttga tcagtggtcc
     3121 aggtgatgat tatgaaaaat ttttagcttt tgtaaaaaca gtggagaagg cgccaggtaa
     3181 tcctattttt gaatggtctc atttggaatt acaacgttac tttgatattg atttgacaat
     3241 ttctgagaag aatgcaccag aaatttggcg acgtgctaac gaaaaaattc aaacggattc
     3301 ctatcgcccg aaagaattgt tggagatgat ggatgtgaaa gccatctgta caacggatga
     3361 tcctacagat gacttgtggt atcatactga gctttctaaa caggataatt ctttcaaggt
     3421 attacctact ttccgtccag ataaggtctg gagtatcaaa tcatcaggct tttcgaacta
     3481 tattttaaaa cttagtcaaa cagcggctat accaatcaaa gactttaatg ggttgaagca
     3541 agcattgagc caacgagtgg atcattttca tcaaattggc tgtcgattag cagatcacgg
     3601 tttgaatact tatttttatg aaatagcaga tgaaaaaata ctcgacaatc tgttaataaa
     3661 agctttagca ggaaaaacgg atttccattt atcagaaatt gcacaatttg ttacaggtat
     3721 tcaattacat ttgatgtctg agtatacaag atatggttgg acgatgcaaa tgcatttaaa
     3781 tgctttaaga aataattcaa gtatattaaa ggaacaaatt ggagtagatg tcggtggtga
     3841 ttcgatgggg gatcaagcta gtttgacaca aaatgttgta gcattgttcg ataccgctga
     3901 aaagagtggg tgcttaccaa aaacaatttt atattcatta aatccaactg attggcttcc
     3961 actagcaaca gcaatgcaaa gcttccaagg gggtatgatt caaaaaatcc aattgggatg
     4021 tgcttggtgg ttcaatgata cagctgaagg aatgcgtcaa cagttgacta cgatggcaca
     4081 acaaagtttg ttgtcgaatt ttacaggtat gttgactgat tctcgaagtt ttctaagcta
     4141 tcctcgccat gagtattttc gtcgagtatt atgtcaatta ctaagcgagt gggttaaagc
     4201 aggccgaatt ccagatgatg aaatgtatct tagtcagata gtggagaata tttgttatcg
     4261 taatgcgtat aattatttta atttttttga aatcggaagt agaaaataat acttgaaata
     4321 aaagcttaaa tttattcttt gtaagttagt acagctagac aaagtatgta tccataaagg
     4381 aaggagatag atatgaaaaa agtagaaatt ttaagtaagt tagaagcggc tggagtcatt
     4441 gcggttgtac gtgggaaaaa taaggatgag gccattaaag caataaattc aattattgcg
     4501 ggtggaatta aagggattga actaactttt acagttccta atgctgaaga aataatcaaa
     4561 gaaattgcta aaaaatatgc tgatcaaaaa gatgtaataa tcggagccgg tacggtttta
     4621 gatgcgttta ctgctcgtat cgcaattatg gctggggcag aatatatcgt tagtccatct
     4681 tttgatcgac aaacagctga gatttgtaat ttatatcaaa ttccttattt accaggttgt
     4741 atgacaatta ctgagataaa ggaggcactt actgctggcg tagatatcat taaactattc
     4801 ccaggttctg cttttggtcc atccattgta aaagcctatc aagctccatt accacacgta
     4861 aatataatgc ctactggcgg agtaagtata gagaacttag aagattggtt tgaagtagga
     4921 gttgttgcag ttggtgtagg tgggaattta ttggcttctg ttgaaaaagg tgattttgaa
     4981 gaagtaagtc gagtagctag taaatatata gataagtttt atgagataaa gaattattag
     5041 gagaaaaaac aaataatact ctgaaatgta aaaaataaat actgactctt tacgcttcag
     5101 agtatagtac gtactttcat cagaatgaaa acgcttaaca acagaaggga gagaattgta
     5161 tggaaagaaa atttggtata agagacaaaa taggatatat gtttggagat tttggaaacg
     5221 attttacttt catttttgct agttcttttt taatgatctt ttatacaaaa gtattgggga
     5281 taagcggagc tgccgttgga acattatttt tagtagcaag atttatcgat gcagttactg
     5341 atgtaactat ggggcgtatt gttgatagat cgaaagcaac taagcagggt aagtttaaac
     5401 cgtggatttt aagaatgagt ggtccagtcg ctttagcaag ttttttaatg tatcaaactt
     5461 ctatgatttc agcttctatg agcgttaagg tagtatacat gtatgttacg tatatattat
     5521 gggggagtat tttttacaca tctataaata ttccttatgg atcaatggca tcagctatta
     5581 cttctattac agaagaaaga acaatgctat cgacattccg aaatgttgga gctactttag
     5641 caagtttggt cattggtgta ttaacaccat tatttattta tacaagagat gattcaggcg
     5701 ctcaaatagt tcgtggtgga tcaactttta caattgttgc gggaatattt tcgatatgtg
     5761 cacttatttg ttatttaata tgttataaac taactactga acgagtaaga attgaccaat
     5821 ctcaatacaa agtttcattg ttaaaatctt ta
//
LOCUS       DASJUF010000140         5844 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.143, whole genome shotgun sequence.
ACCESSION   DASJUF010000140 DASJUF010000000
VERSION     DASJUF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.143"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(525..1028)
                     /locus_tag="U4B62_002593"
     CDS             complement(525..1028)
                     /locus_tag="U4B62_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2142702.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="U4B62_002594"
     CDS             1387..2412
                     /locus_tag="U4B62_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2142703.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2735..3613
                     /locus_tag="U4B62_002595"
     CDS             2735..3613
                     /locus_tag="U4B62_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN2142704.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4089..4205
                     /locus_tag="U4B62_002596"
     CDS             4089..4205
                     /locus_tag="U4B62_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2142705.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5133..5471
                     /locus_tag="U4B62_002597"
     CDS             5133..5471
                     /locus_tag="U4B62_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142706.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5565..5795
                     /locus_tag="U4B62_002598"
     CDS             5565..5795
                     /locus_tag="U4B62_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142707.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1988 a          880 c         1072 g         1904 t
ORIGIN      
        1 aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2521 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2581 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2641 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2701 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2761 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2821 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     2881 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     2941 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3001 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3061 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3121 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3181 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3241 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3301 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3361 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3421 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3481 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3541 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3601 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3661 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3721 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3781 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3841 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     3901 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     3961 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4021 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4081 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4141 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4201 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4261 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4321 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4381 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4441 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4501 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4561 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4621 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4681 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4741 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4801 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4861 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     4921 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     4981 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5041 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5101 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5161 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5221 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5281 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5341 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5401 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5461 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5521 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5581 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5641 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5701 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5761 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5821 gacacatatt atgttattgt tttt
//
LOCUS       DASJUF010000141         5836 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJUF010000141 DASJUF010000000
VERSION     DASJUF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.144"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..382
                     /locus_tag="U4B62_002599"
     CDS             <1..382
                     /locus_tag="U4B62_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HEN2142708.1"
                     /translation="YYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVEN
                     NHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFC
                     LTGITAWNTFVELKKKRRRTQKFL"
     gene            510..1193
                     /locus_tag="U4B62_002600"
     CDS             510..1193
                     /locus_tag="U4B62_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN2142709.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1502..1575
                     /locus_tag="U4B62_002601"
     tRNA            1502..1575
                     /locus_tag="U4B62_002601"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1536..1538,aa:Arg,seq:tct)
     gene            1734..2093
                     /locus_tag="U4B62_002602"
     CDS             1734..2093
                     /locus_tag="U4B62_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN2142710.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2216..3847
                     /locus_tag="U4B62_002603"
     CDS             2216..3847
                     /locus_tag="U4B62_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN2142711.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3909..4550)
                     /locus_tag="U4B62_002604"
     CDS             complement(3909..4550)
                     /locus_tag="U4B62_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2142712.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4736..4812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4832..5140
                     /gene="rplU"
                     /locus_tag="U4B62_002605"
     CDS             4832..5140
                     /gene="rplU"
                     /locus_tag="U4B62_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN2142713.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5167..5511
                     /locus_tag="U4B62_002606"
     CDS             5167..5511
                     /locus_tag="U4B62_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN2142714.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5524..5817
                     /gene="rpmA"
                     /locus_tag="U4B62_002607"
     CDS             5524..5817
                     /gene="rpmA"
                     /locus_tag="U4B62_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN2142715.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1814 a          985 c         1257 g         1780 t
ORIGIN      
        1 atattatgca caaggaaatc tgatatatgt acgaccgaaa aatacaaatg agattttagt
       61 cggagatatt attacatatt atgaaaattc aggaaaaaaa atctcgacgc gtcgcgtaat
      121 agccgttgaa aacaaccacg aagattttta tacaaaagct gatacaaaga cttatattga
      181 gcctggagta gtaaaaaaga gaaatattat cggtagtccc atctttcaga ttccatatat
      241 tggactagtc ctttcaaaaa cggcgtttgc atggattcag gggttattct ttacgattgc
      301 tttttgccta acaggaatca cagcatggaa tacctttgta gagttgaaaa agaagcggag
      361 acgaacacag aaatttttgt aagaatgtta aaaacataaa tgtattccag agatccaacg
      421 actcttctga acatgaaaca cgagaaaaca ctcatgcagc ttgtagattt agtcagttgt
      481 tcgtataatg tgtgaagagg aggagtagta tgcgcaatga aatgatgcat cgtcctgtag
      541 tcaaagaaga actattggat tttatgcgta cccagcaaaa aaaattaccg ggtgagctag
      601 gcaaactgga agaagaagct catgtagcag aagtaccagt tattccccat gaaacagttg
      661 tatttttgaa atttttactt ggacagcttc agcctgaacg aatcttggag atcggagcag
      721 ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg agaaaacgct catgttacga
      781 caattgatcg tttcgatgtc atgatcgaga aagctaaaaa aaactatgaa cgtctaggac
      841 tgacagataa agtgactttg cttgaaggtc aagcagcaga tatcttgccg gagttgtctg
      901 gtccgtatga ctttattttt atggatagtg caaaatcaaa atatattgaa tttttacctg
      961 aatgcttacg tttgttaaga aaaggtggcg ttttgatggt agatgacatc ttccaaggag
     1021 gaacgatctt gctcccagat gaagagattc cccgtggcaa acgagctatc catcgtaaat
     1081 taaatgaatt tcttcgtgtt gtgatggatc atcccgattt gacatcgaca atcttgccat
     1141 taggtgatgg tgtgatctta atgacaaagg aatcagaaac aatcgagttg tgatacatga
     1201 ttatggcagc ctgtagataa tcgattgaat cgagggctca attcgaactt gatgtaaaag
     1261 tttctatatt tgtaagtcaa ttaaaggata caggaaatct aaataaacag gagtagcatg
     1321 aagtcaatgt agctcctgtt ttcaattgat aaccttagaa cagctgaagg agaatgtctc
     1381 gttttaaaag attgatttag tagttgggcc aaaataattc attatttgct aattaaataa
     1441 ctatttcgta ttgacagaag caatttaatt atctataatt attgatgttc cgctgattct
     1501 tgtcatagta gctcagctgg atagagcatc cgccttctaa gcggacggtc gggggttcga
     1561 atccctcctg tgacgctaaa aaaccgttag aaactgaggt ttctaacggt ttttctttgt
     1621 tttttgtgcc aatattgttt gttttcaaag gcaaataaaa taaaaaaaca tccttatcga
     1681 gtacaattat aaggagcaaa aaaataaaac tcatcagaaa agaggaagaa gatatgacga
     1741 tactatctag aattttagtg actctagttg caattgaatt tttttatatc atgtatatcg
     1801 aaacggtgag aactgattct gacacgacaa gtagggtttt taaaatgagt aaagaagagt
     1861 taagcagaaa atctgttcaa acattgttta agaaccaagg agtgtacaat ggactattag
     1921 gagtgggact actttatgga gcttatcttt cttccgcatc taaagaaatc acaagtatgc
     1981 tattaatatc tatttttttt gttgcattat atggcagttt gactagcgat cgttggattt
     2041 tactcaaaca aggtgggcta gctctgttgg cattgctttc tattttctta taaatataga
     2101 aaaaataaga atttatctaa caggaaactt tgtgaaaaat attactttat ctcatgaaat
     2161 atgatattct aatgaatggt attttagaaa atttcatgaa aaaggagtta gataggtgtt
     2221 caaaaattat gtttcctcac taaaaaaaga attttcaggt tatgatcttt caagatttca
     2281 gaaagatctt ttggctggga tcacagtagc tgctgtagca ttgcctttag ccttggcttt
     2341 tggggtatca agcggagctg atgcggccgc aggtttgatt acggctatca ttgccggtat
     2401 cgttattggc ggtttatcag gcggttttta tcaaatatca ggtccgacag gtgcgatggc
     2461 agctatcttg atgtctattg cggcaacaaa gggaatgcaa ggtgtattgc ttgctacttt
     2521 tttagcgggt ctatttttac ttgcagcagg acttttccga ttaggaacac tgacttcttt
     2581 tatcccagca cccgtaatta cggggtttac ttcaggtatt gctatcatta ttgcattagg
     2641 tcagatcgat aatttatttg gtgttcactc ggaaggtaca aacgttattg aaaaactaag
     2701 tagttatcaa acgataggat ttccgataaa cacagcctct ttgatcatgg gagggctagt
     2761 cattttaggg atgtttatct atccaaaaaa atgggcgaaa cgaatgccta gttcgctgct
     2821 ggctatcatt ttggcaacag cagttatgct attgttccat ttaccagtag caactgtggg
     2881 taagattcca caaacattga tcagtagtaa tcgtttgaat atgggagatt ttagcttttc
     2941 tgccttgcaa caagtggcag ttccagctat tagcattgct ttattaggaa tgattgaaag
     3001 cttgctatgt ggtgcttcgg ctggccgaat ggcaaataag cccttagata gcaatcaaga
     3061 attaatggcg caaggaattg gaaatctgtt gcttcctttc tttggcggga ttcctgctac
     3121 tgcggccatc gcccgtacaa gcgtggccat caaatctggt gcacaaacta gattagctgg
     3181 tatgatacat gcagttgtct tactgctctc catgcttgtt tttgcgccag tgatgtcaca
     3241 gattccaatg ccagcacttg caggtgtttt gattgtgacg gcttggcgta tgaatgaatg
     3301 ggaaacgatc aaggagctat ttactaataa atattggtca gcagttctat tgtttatcct
     3361 tacgatgatt tgtacagtta tctttgattt aagtattgct atcgtgatcg gggtgatcgg
     3421 cggctgtgta ttttttgttg tcaaaagcgc tgctatcacg atttcattag aaacgatcga
     3481 ttggcaaaga atggggctgc cagaaacgga aaagctgaat aactggacag tagtgtatat
     3541 tagtggtcca ctgtttttta tgtcttcaga aaaactcaaa aataaattgg aagaactaaa
     3601 tcatcacgat ggtgtcattt tctccatgcg aggcgtacca agtattgatt tgacggcacg
     3661 tgcagttttt gatgaatttc aagaaaaagc agagcaaaga ggacaaacga ttatctatac
     3721 ttccttgcag ccagaagtag aaaaacaact ttctcacttg tgggaacata cattcacgca
     3781 tcaacatcaa acagttgctc atgcgttgac agctttacat gcgaaatata ctgcaattag
     3841 ccattaaaac aatgaaaaag aacagacaag actgtaaact gaagatttta tggtcttgtc
     3901 tgttcttttt aatattgctg taaaagtgaa gctagtactt gccagtcttg atggataaca
     3961 ggtacaaggg agcgattgta gaaaatagct gctggatgaa aaagtggaag aattcgatag
     4021 tttttttctg accattcgta ggcatccttt tgttctgtta attgtaagac aggccctgta
     4081 tagagttgac catgattttt ggtaaccgta tattctttgc caagtaaacg ttgaagacca
     4141 atgtttccaa gtgtcgcaat taattttggc tggaccttca tcagttcgta atccaatatt
     4201 ggtgcatgcg caaatacttc ggaacgagta ggtgtccgat ttggataaac agtttctgac
     4261 tgttgtgtcc gtttattgat ccgatgagtc acgcgatacg ggcggctcct tacagcactg
     4321 gtgatataca cctcttctcg tgttaagcca gttgaagaaa gtgcctgcat caattctttt
     4381 ccagcttgac cgctaaaagg aacatgcgta tcaatctctg ttttccctgg ggcttctccc
     4441 acgagcatca gtttaggatg aagaggtcct tgcccagcga taaatccttc tacaggacgg
     4501 ccgttcattc ttaaagaagc ttcttgtatc agttcttttg ggtattccat ttatgctcct
     4561 cctttttctt ttattataag aataaaagaa acacaatgcg actattttat ttggtagaac
     4621 tgtgaggata gaaaagcaga agtattgctt gaatttttgt tttacttttt gattttaaat
     4681 ttagttgact aacggttgct gttctggtaa gctagtactg ttgtaattgt ggactccaca
     4741 gctacaaccg ctcagaacaa gtaataagca cagcatatgc tgtcacttcg gatggcgagt
     4801 ctaagtatat cagaaggagg tgctttatag catgtacgca attattaaaa ctggtggtaa
     4861 acaagtaaaa gttgaagtgg gacaagcaat ctatgttgaa aaacttgacg ttgaagcagg
     4921 agaaaaagtt gtttttgacg aagttatctt agtaggcgga gaatctacaa aagtaggcgc
     4981 tccaactgtt gcaggtgcaa ctgttgaagg tactgttgaa aaacacggca aacaaaagaa
     5041 agtcgttact ttcaaataca aacctaaaaa acattcacac cgtaaacaag gtcatcgtca
     5101 accatataca aaagtggtta tcgacgcgat caacgcttaa tagcgtttga gaaagaaggc
     5161 ctatatatga tcaaaggaac atttaagcga aatgacgcag gtcagatcgt ttcattcact
     5221 ttgaccgggc atgctgatgc tggtccttat ggaagtgata tcgtttgtgc gggtgtttct
     5281 gcgttagcga tcagcactgt caacgggatt gcctcattag ccggttttga gccgattgtt
     5341 gaaatgaatg aagaagaagg cggttatctt tataccgaag tcacttcagg aatgacacaa
     5401 gaacaaaaca atattgctga gattctatta gaaaatcttt tattaggatt acaatcgata
     5461 gaagctgaaa actcagaata tatccaaatt aaaacaatta atgataaata ggaggtgcag
     5521 accatgttgt tatctatgaa tctacaatta tttgcccaca aaaaaggtgg cggttctaca
     5581 tctaacggcc gtgactcaga atcaaaacgt ctaggtgcta aacgtgctga tggacaaaca
     5641 gttacaggtg gatcaattct ttaccgtcaa cgcggtacaa aaatctatcc aggtgtaaac
     5701 gtgggtatcg gtggagacga cactttattt gcaaaagttg atggcgtagt acgtttcgaa
     5761 cgtaaaggcc gtaacaaaaa acaagtgtct gtttatccag tagctcaaga agcttaattg
     5821 aatcttatga ttaaag
//
LOCUS       DASJUF010000142         5805 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJUF010000142 DASJUF010000000
VERSION     DASJUF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.145"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            122..493
                     /locus_tag="U4B62_002608"
     CDS             122..493
                     /locus_tag="U4B62_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142716.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            578..778
                     /locus_tag="U4B62_002609"
     CDS             578..778
                     /locus_tag="U4B62_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142717.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            912..2138
                     /locus_tag="U4B62_002610"
     CDS             912..2138
                     /locus_tag="U4B62_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142718.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2735..3130
                     /locus_tag="U4B62_002611"
                     /pseudo
     CDS             2735..3130
                     /locus_tag="U4B62_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            3208..4247
                     /locus_tag="U4B62_002612"
                     /pseudo
     CDS             3208..4247
                     /locus_tag="U4B62_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            4260..5198
                     /locus_tag="U4B62_002613"
                     /pseudo
     CDS             4260..5198
                     /locus_tag="U4B62_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            5191..5538
                     /locus_tag="U4B62_002614"
     CDS             5191..5538
                     /locus_tag="U4B62_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142719.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT         2154 a          838 c         1135 g         1678 t
ORIGIN      
        1 tttatttgga taatcatgtg aatatgaact taacaattaa acgtatttac gatcttttat
       61 cacttatata aagagagaag tgataaatag gacacaaaat ttggaaagta ggtaagtcac
      121 tatgagagaa ctaactttat cttctaagga attagaaaag aaaactgctt tattaaacga
      181 aatatttagt gctgattgta taatccaaag tttaaaagaa agttcatttt caaaactaac
      241 agatcaggaa aaaatttctt atatgctaag tttagataat ctaggaacta aatatgatga
      301 atcagcaagt agcgataagc ttttgattga attagtatca agtcttagta gttttggaaa
      361 aatgaaaaga tttaatgtga tattagaaat ttttgcttgg ttaatccctt catttatttg
      421 gtttttttat attttcatag ttgaatctga gttaatagcc aaggtctctc ttggactgtt
      481 aattccgata taaataggtt cagcaattta ctttaataac ttgtatacac ctattcaaat
      541 aaaaaagcag ttattacatg aaggattaag tggtaatatg ttaaaaagaa ttgcatggga
      601 taaagataat aaaacagata ttgagcttaa agaatataaa tacaattatt tctcgagatg
      661 tatagataga aagttactgt ttaaaaaaga aggattagaa tttagttttg atgaagatga
      721 atttgaaaaa ttagtgatgg actggcgtaa gaatttactg gatacggtac aattctaaaa
      781 gcaattgagg tcgaaattcc gatacttgaa tcatgatcta tattatcgtg ttatgagtga
      841 aatttctaat aaagataaga tatttcattt ggacagtcat gtgaatatga gcttaacaat
      901 taaacgtatt tatgatcttt tatatgttta taaacattaa tgaggtgcta aaattgacaa
      961 caataactat gacaaaaaaa atagaagtat atgttgatgg atcacaagaa gaaagggatt
     1021 ccttttttga ttttttttgg aaaaatagta gagaatatgt aaaagcgttg aatctggtat
     1081 ataaacagct aatgtttgaa gagttgataa aagatgcaat taaagaaatg gatcaaggtt
     1141 atgctgagag agaagaaagt attcaaaatc ggattgaaaa gttagcatat actgatccaa
     1201 tagatcaaaa aaaacttgat aaagcaaaag ctgaattatc taaattgaga aatgaaaaaa
     1261 ataaagaagc gagagaaata ttaacgcaag cgatagggtt gaagagtcaa acgagaacac
     1321 gagatgtagc aaaaaaatta gactttgact ttggagattt tgttgattcc gcaaatatga
     1381 aagctagtaa agattttggc aatgatttta tcggaattgt gaccggacaa agaacgccta
     1441 gaatatataa aaatagaaac aaatatggat tattaccttt tagaggtcga gatattaaaa
     1501 tccataaaga tgataagttt tatatttatt tacctaaagg ctttaggttt aaagtgaatt
     1561 taggtagcaa gccaagaaaa gcaagagatg ttgcttcagt tttacaacac attatggatg
     1621 gcgattacaa attaaatcaa agtaatctta tttttgatgg aagaaaatta ttttttaatc
     1681 taacaatttc ttttgaaaaa agctataatg aaatggaatt caagaaaggt aatgaattga
     1741 tcgtaaactt tgcagatttg tgtagcggtg aatgtttaat taatgataaa tctgtaatgt
     1801 cttttggtga tagccaatat ataagaaact tcaaaagtaa aattttagca atgaaatcaa
     1861 aagaacaaag ccgaggagtc ttttctaagg gcggtcatgg tcgaaaaaga aaattagatc
     1921 aagatcgatg ggaagcaata aagaaaagag aaagtaattt tgttaaaact tataataatc
     1981 aatgttctgc aaaaatcgtt aaaactgcta ttcgcaataa agctcaaatt attcgtatca
     2041 ttgaaccaga taaggcttat ggcgaatggg cttactatca attgaaagaa caactagtaa
     2101 acaaaggaaa aagatatggg atagaagtca gaagttagta aattaaacaa tcgttgattt
     2161 ggcaacgaaa tagtgaggga aaatccactc actgaagggt aaactccgtc aagttgtgtg
     2221 accagacgga aggacgaatg ttgttgaggt tttagaacca tataaaacag catgtttctc
     2281 aaatttttag gataagggaa attgctagca gtttgtttta gaaccatata aaacagcatg
     2341 tttctcaaat gagtacttta aattaattga tggcgttcct gctttagaac catataaaac
     2401 aacatggatt taaaacaaga agtaagtcag ttctcctttt actttagtaa ggaaaaataa
     2461 agtattctca ataaaactta aaataagcaa acctttgttt taaaatgatt gtcaagttaa
     2521 aaaaatctgg gatcatataa aatagtatag ttctcaaata tgattagaaa ccctattaaa
     2581 gagcaatatt aagccaactt acttaaatcg tttaattaag aacaataatc tcttttagtc
     2641 agacttttag tctgactttt ttgtatttcc aaaatttatt ctttacaaaa acgaacatac
     2701 gtttgtataa ttacttgtga atgggggaat aaaaatgttt gattacagta gagaaccggt
     2761 gaatgactat tttttaatcg acatgaagtc tttctatgct agcgtggaat gtattgaacg
     2821 aaatttagac ccaatgacta caccgttaat tgtcatgagt agatcggaca acactggttc
     2881 aggtctgact ctagcttctt cacctactgc aaagaaaaaa tatcatatta ctaatgttag
     2941 tagagctact gatttacctt atccattacc aaaagaactt catattgttc caccacgaat
     3001 gaacttatat ataaaaaaaa atatggaagt taatcagatt tttagaaagt atgtcgcaga
     3061 tgaagattta ttgatttatt cgattgatga atcaatttta aaagtgacaa aatccttaaa
     3121 tttatttact atataaattg agttaaattg tattcgtttt tcctcctata ggcgtatact
     3181 aaagaaaaca cttgaggagt ggggaacatg gaaacagaac gccaaatccg tgaattggaa
     3241 aaagcgatga atacgacaga caaccgccgg ttgtacgagc gtttcctggc agtcaagttg
     3301 gttcttgaag gccacacccg gaaagaagcc gggcacacca tcggccgcga cgagcatact
     3361 gtcagccact atgtgaaaaa ctactgccat aatggaatcc aaggtttagt ctccaaaaaa
     3421 caaagcggac gaccttcacg gctgacagat gaacaggaag cggaactggt ccaaatcgtc
     3481 gcctaccaca ctccggagga agtaggtttc aaatcccgtg ccaattggac attggcgatt
     3541 gtgtgtgacc tgattctccg ggaatggagt tatggatata cgcaaaaagg cgtggcggac
     3601 ctccttcacc ggctaggact aagctggacc cgtccgacgt ataccctcaa aaaagcggat
     3661 tccaagaaac aacaggtatt tttggaagaa accttcccga gcctaaaaaa aaattgctga
     3721 atagcgatat tgaccacctc ctattcgagg acgaaaccat gatccgggat taccaagcca
     3781 ttggctgcac ctggtttctc cgcggccacc agcgaaaaat ccccacttat ggacaacaca
     3841 aaggggtaaa attgataggc accttggatt atgaaaccgg tcggatcatt tgtacagaag
     3901 aaggggcata cgacgcagcg gccttcctcc gttttctgga gcggacgttg cgggaatacc
     3961 ccaccggaaa aatcgtgatg gtattggata atgcccttat ccaccacgcc aagctgatcc
     4021 agtcttttct tgaagaaaac gctaggcgcc tggcactcat cttcctccct ccctacagcc
     4081 ctcagttgaa cctaatggaa ggcgtttgga agtggctcaa ggaatcggtc atcaacaatg
     4141 ttttttttga ccacgtccag aaaatcaaac agtcggtccg cggtttctta gccgatgtaa
     4201 acgagcgtcc ccttgaggtg attgaccgac tgtgcgtgcg gatgtaaagt atttaattca
     4261 acctatatag ttcaggaaca agaagcgaga gaagaagaga attagctaaa ataatcaaag
     4321 atgagattca gaaagaaaca ggtctaatag caacagtcgg aataggagat aacccattat
     4381 tagctaaact tgcactggac aatgaagcga agagtagtaa agaatttata gctgagtgga
     4441 cttatgagac tgtacctgaa aaaatatgga aaataaacga gatgactgac ttttggggca
     4501 ttggatcacg catgaaaaaa agattgaatc atatgggaat tatgagtata gaagatttag
     4561 caaattggaa tccctacaca attaaaagtc agttaggtgt aatcggactc cagttatatt
     4621 tccataccaa tggaattgat cgtacggata ttgctattcc accagaacca accaaagaaa
     4681 agtcttatgg gaattcacaa gttctaccaa aagattatac gaggagagaa gaagtagaac
     4741 tagtagtcaa ggagatggca gagcaagtag ccattcgaat tagacaacac aattgcaaaa
     4801 ctggttgtgt acgcttagca attggtacgt caattttgga agaaaaatca ggtttctcgc
     4861 accagatgaa aataccaatt acagataata cgaaagaatt acaaaattat tgtctttttt
     4921 tattcaataa gttttatgag gggcaggaag tacgacatat aggagtaact tatagcagat
     4981 tagtatacac agattcccta caattagatt tatttagtga tccaagaaaa aagattgctg
     5041 aagaaaattt ggataggatt attgataaga ttagacaaaa atatggattt acagctatta
     5101 tacatgcttc atctatgtta gaaggtgcaa gaagtattac gagaagcact ttaataggag
     5161 gacacgcagg aggattagga ggtttgcata atgattgaat ataacgatag aggacgttta
     5221 aaatggtcgg gattttattt gtctgatcat actgaaagaa tcaatgcaga caatgtacaa
     5281 cgtgctaatc ataatttggc aaaagaacaa atgacgactg aagagattgc gaaaatactg
     5341 agaactgctg tcataaaaaa tagacccgtc tgcattcaaa aagaggaacg aaacgctgaa
     5401 ggttactatc cagatgatgt agtaggaaaa attattggat atgacgagct tggtgtattt
     5461 gtggaagaag aaaaagttca ctatgatgaa attaggaatg tttctttctc ggaattatta
     5521 aaatggagtc agatatgaac attattaaaa aatacgaatt cggttatatt acgtatgaac
     5581 agctaataga ggaattatgg ggatatggtc aacaattaat taatgaagtg gggattgatt
     5641 gcttttgttt ctatattgaa tccggagcag gataccataa gaacagatac tatatatttc
     5701 catatccacc aaaatagtct cttagatatt tttaaatttt ctccaaatac tttcaattat
     5761 tgtgaaatat ttccctttaa ttaatcaaat tttttataaa agtaa
//
LOCUS       DASJUF010000143         5771 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJUF010000143 DASJUF010000000
VERSION     DASJUF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.147"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1016..1306)
                     /locus_tag="U4B62_002615"
     CDS             complement(1016..1306)
                     /locus_tag="U4B62_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142720.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="U4B62_002616"
     CDS             1708..1944
                     /locus_tag="U4B62_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142721.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="U4B62_002617"
     CDS             1951..3903
                     /locus_tag="U4B62_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2142722.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="U4B62_002618"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="U4B62_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN2142723.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4799..5491)
                     /locus_tag="U4B62_002619"
     CDS             complement(4799..5491)
                     /locus_tag="U4B62_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2142724.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1895 a          997 c          917 g         1962 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     4561 taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa attttcattc
     4621 aattatcatt ttcaattgca ttttataaat cattttgtat gtaatttggt atgtaagtta
     4681 aaatgaaaaa agtagtccat tttgaagtac attttgtcat tttgtactcc aaaactgaac
     4741 tacttagtat agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt
     4801 atttacgcaa atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat
     4861 cttttgcttt ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt
     4921 cgtctaattt cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca
     4981 gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt
     5041 gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt
     5101 ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct
     5161 tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt
     5221 ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct
     5281 gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg
     5341 tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca
     5401 aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga
     5461 caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta
     5521 catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catatgtaat
     5581 tttaatataa atactatcaa atactcattt aatcctcctt ttttatctat ttaaaaaatc
     5641 ttttttatag agaaaaatgc aggttaatac tttcttaaat aaatatttat ggtgaacaaa
     5701 aacaaaaaag caagttttat taaacgtaac ccttttcatt tcatagatta ctgtatttat
     5761 tacaaaaaac g
//
LOCUS       DASJUF010000144         5689 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.148, whole genome shotgun sequence.
ACCESSION   DASJUF010000144 DASJUF010000000
VERSION     DASJUF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.148"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(488..1297)
                     /locus_tag="U4B62_002620"
     CDS             complement(488..1297)
                     /locus_tag="U4B62_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2142725.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="U4B62_002621"
     CDS             complement(1297..2043)
                     /locus_tag="U4B62_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2142726.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="U4B62_002622"
     CDS             complement(2061..2528)
                     /locus_tag="U4B62_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2142727.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="U4B62_002623"
     CDS             complement(2528..2938)
                     /locus_tag="U4B62_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2142728.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="U4B62_002624"
     CDS             complement(2949..3962)
                     /locus_tag="U4B62_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2142729.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="U4B62_002625"
     CDS             4179..4913
                     /locus_tag="U4B62_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2142730.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="U4B62_002626"
     CDS             4897..5607
                     /locus_tag="U4B62_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2142731.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DASJUF010000145         5569 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJUF010000145 DASJUF010000000
VERSION     DASJUF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.149"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..862
                     /locus_tag="U4B62_002627"
     CDS             5..862
                     /locus_tag="U4B62_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2142732.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            884..1393
                     /locus_tag="U4B62_002628"
     CDS             884..1393
                     /locus_tag="U4B62_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2142733.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1430..1831
                     /locus_tag="U4B62_002629"
     CDS             1430..1831
                     /locus_tag="U4B62_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142734.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1943..2296
                     /locus_tag="U4B62_002630"
     CDS             1943..2296
                     /locus_tag="U4B62_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142735.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2330..2773
                     /locus_tag="U4B62_002631"
     CDS             2330..2773
                     /locus_tag="U4B62_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2142736.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2790..3875
                     /gene="uxuA"
                     /locus_tag="U4B62_002632"
     CDS             2790..3875
                     /gene="uxuA"
                     /locus_tag="U4B62_002632"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2142737.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3868..4581
                     /locus_tag="U4B62_002633"
     CDS             3868..4581
                     /locus_tag="U4B62_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2142738.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4788..4937)
                     /locus_tag="U4B62_002634"
     CDS             complement(4788..4937)
                     /locus_tag="U4B62_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142739.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5333..5533
                     /locus_tag="U4B62_002635"
     CDS             5333..5533
                     /locus_tag="U4B62_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2142740.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1934 a          854 c         1210 g         1571 t
ORIGIN      
        1 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
       61 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
      121 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
      181 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
      241 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
      301 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
      361 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
      421 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
      481 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
      541 cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga cagcgattga
      601 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
      661 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
      721 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
      781 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
      841 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
      901 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
      961 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
     1021 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
     1081 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
     1141 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
     1201 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
     1261 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
     1321 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
     1381 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
     1441 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
     1501 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
     1561 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
     1621 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
     1681 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
     1741 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
     1801 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
     1861 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
     1921 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
     1981 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
     2041 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
     2101 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
     2161 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
     2221 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
     2281 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
     2341 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
     2401 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
     2461 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
     2521 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
     2581 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
     2641 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
     2701 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
     2761 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
     2821 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
     2881 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
     2941 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
     3001 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
     3061 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
     3121 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
     3181 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
     3241 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
     3301 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
     3361 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
     3421 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
     3481 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
     3541 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
     3601 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
     3661 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
     3721 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
     3781 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
     3841 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
     3901 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
     3961 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
     4021 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
     4081 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
     4141 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
     4201 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
     4261 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
     4321 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
     4381 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
     4441 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
     4501 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
     4561 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
     4621 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
     4681 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
     4741 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
     4801 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
     4861 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
     4921 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
     4981 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
     5041 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
     5101 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
     5161 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
     5221 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
     5281 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
     5341 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
     5401 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
     5461 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
     5521 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaa
//
LOCUS       DASJUF010000146         5480 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJUF010000146 DASJUF010000000
VERSION     DASJUF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.150"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="U4B62_002636"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="U4B62_002636"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="U4B62_002637"
     CDS             complement(580..1353)
                     /locus_tag="U4B62_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2142741.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="U4B62_002638"
     CDS             1497..1847
                     /locus_tag="U4B62_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN2142742.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="U4B62_002639"
     CDS             1828..2544
                     /locus_tag="U4B62_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2142743.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="U4B62_002640"
     CDS             2544..3992
                     /locus_tag="U4B62_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2142744.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="U4B62_002641"
     CDS             complement(4062..4571)
                     /locus_tag="U4B62_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142745.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="U4B62_002642"
     CDS             complement(4561..5286)
                     /locus_tag="U4B62_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142746.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1566 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt
//
LOCUS       DASJUF010000147         5433 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.151, whole genome shotgun sequence.
ACCESSION   DASJUF010000147 DASJUF010000000
VERSION     DASJUF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.151"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            69..887
                     /gene="purR"
                     /locus_tag="U4B62_002643"
     CDS             69..887
                     /gene="purR"
                     /locus_tag="U4B62_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN2142747.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1390..2763
                     /gene="glmU"
                     /locus_tag="U4B62_002644"
     CDS             1390..2763
                     /gene="glmU"
                     /locus_tag="U4B62_002644"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN2142748.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2857..3828
                     /locus_tag="U4B62_002645"
     CDS             2857..3828
                     /locus_tag="U4B62_002645"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2142749.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3834..3936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4002..4571
                     /gene="thiT"
                     /locus_tag="U4B62_002646"
     CDS             4002..4571
                     /gene="thiT"
                     /locus_tag="U4B62_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN2142750.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4652..5425)
                     /locus_tag="U4B62_002647"
     CDS             complement(4652..5425)
                     /locus_tag="U4B62_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142751.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1785 a          959 c         1161 g         1528 t
ORIGIN      
        1 gctcgaattt ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt
       61 tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga
      121 atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat
      181 cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa
      241 ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg
      301 aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc
      361 ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag
      421 ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag
      481 ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta
      541 ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg
      601 gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac
      661 gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct
      721 tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca
      781 acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc
      841 aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa
      901 agccgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca
      961 taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa
     1021 atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc
     1081 cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta
     1141 agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt
     1201 atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata
     1261 ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact
     1321 tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga
     1381 gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg
     1441 aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc
     1501 attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca
     1561 gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg
     1621 ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg
     1681 ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa
     1741 tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca
     1801 ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa
     1861 gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac
     1921 aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac
     1981 tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa
     2041 acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat
     2101 gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat
     2161 tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca
     2221 ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca
     2281 aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc
     2341 agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa
     2401 attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa
     2461 ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat
     2521 gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc
     2581 ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa
     2641 aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca
     2701 attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac
     2761 taagactttt gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag
     2821 aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca
     2881 agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca
     2941 gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta
     3001 aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca
     3061 gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca
     3121 aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt
     3181 gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca
     3241 agaatgctga ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc
     3301 gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc
     3361 gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg
     3421 gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca
     3481 gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg
     3541 atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact
     3601 agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa
     3661 gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa
     3721 atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc
     3781 catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca
     3841 caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc
     3901 ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt
     3961 cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt
     4021 gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga
     4081 tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa
     4141 gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg
     4201 gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt
     4261 ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata
     4321 cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt
     4381 tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc
     4441 cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga
     4501 tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac
     4561 aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg
     4621 tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg
     4681 catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt
     4741 cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg
     4801 ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct
     4861 gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt
     4921 ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt aatttactag
     4981 caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa
     5041 gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt
     5101 tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg
     5161 ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg
     5221 aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat
     5281 attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac
     5341 tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa
     5401 tatgtacata aactgactca gtcatcatgt tcc
//
LOCUS       DASJUF010000148         5312 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJUF010000148 DASJUF010000000
VERSION     DASJUF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.152"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B62_002648"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B62_002648"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN2142752.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="U4B62_002649"
     CDS             complement(1297..2133)
                     /locus_tag="U4B62_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142753.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="U4B62_002650"
     CDS             complement(2317..3534)
                     /locus_tag="U4B62_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2142754.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="U4B62_002651"
     CDS             complement(3683..3862)
                     /locus_tag="U4B62_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142755.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="U4B62_002652"
     tRNA            3917..4000
                     /locus_tag="U4B62_002652"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="U4B62_002653"
     CDS             complement(4240..4818)
                     /locus_tag="U4B62_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142756.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4824..5207)
                     /locus_tag="U4B62_002654"
                     /pseudo
     CDS             complement(4824..5207)
                     /locus_tag="U4B62_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DASJUF010000149         5188 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJUF010000149 DASJUF010000000
VERSION     DASJUF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.153"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            136..747
                     /locus_tag="U4B62_002655"
     CDS             136..747
                     /locus_tag="U4B62_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2142757.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            820..1749
                     /locus_tag="U4B62_002656"
     CDS             820..1749
                     /locus_tag="U4B62_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN2142758.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1818..2644
                     /locus_tag="U4B62_002657"
                     /pseudo
     CDS             1818..2644
                     /locus_tag="U4B62_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2692..3870)
                     /locus_tag="U4B62_002658"
     CDS             complement(2692..3870)
                     /locus_tag="U4B62_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2142759.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3968..4066
                     /locus_tag="U4B62_002659"
     CDS             3968..4066
                     /locus_tag="U4B62_002659"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2142760.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4097..4498
                     /locus_tag="U4B62_002660"
     CDS             4097..4498
                     /locus_tag="U4B62_002660"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2142761.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4705..>5188)
                     /locus_tag="U4B62_002661"
     CDS             complement(4705..>5188)
                     /locus_tag="U4B62_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2142762.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1638 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaatgta ttataggcat
       61 ttttttacaa gaaatagaag cttgaagttt atatgaagtt ataaaattta atttatattg
      121 gaaggaggaa aataaatgta ttttatacta cctacacttt atagcctttt atgtgtaatg
      181 gcaccatgta ttatttatat ggtatttttt tgcaaaaaaa ataccctgaa acacaatatc
      241 ctgattttga tttttatgct gtactgttgg aaagtttttg aattgactgg tacaggtgga
      301 ctaacagata ttttttatag acctgataca ggtaggaaat tttctctagt acagggcagt
      361 gttaatcttg taccattcag tggactaaac attagcttta tattaaatat agtaatgtgt
      421 gtgcccttag gattcttact accaatcata tggaaaagtt ataggaaaat agttcctgtt
      481 ttcttggctg gggctctatt ttcactaatt attgaagtct cacaaattat aactacaaga
      541 gcaacagata ttgatgattt aatagcaaat acctttggtg cggttatagg ctttatactg
      601 tgggctgttt taagtagagt ggtgtccttg aaggacaagt catctacagt agattattta
      661 gaaccttata tttatatatt gattggtttt accggaaaat tcttactgta ttttcctttc
      721 tggtttgctt ttgaagtatt atcctgataa ttccatagaa aataatgagt ttgagttgag
      781 aaagaatatt cttgtacaca actctggaaa ggaggaagta tgaaaagatt cttaaaatgg
      841 gggcttgttc ttttagcgtc tttgattgtg attctaatgt gtacagcggt tgttatttct
      901 tatgtgaatc ggccgtatgc aagagctgtt tatgatagga ttaaacgcat tcttgacaaa
      961 gatttttatg accatctata tctaggttct tcatttgaaa taataaacct tgatacaatg
     1021 gatattcata gctatatgaa aaaagaccaa gaagaattac ccgcctcatt gagcaaatta
     1081 tttacgattg attatgcgct tagtgtccta agtttagatg aacagattac aattagtcct
     1141 actgcacttg acatgcagaa agctgactca tctactgcac atcttcaaga aggagatatt
     1201 ttatctgttt atgacattat ctgtgctatg gtcttaccct caggaaatga tgcagcttat
     1261 agtttggcaa tttatacagg tggaaaactt gatcctgatg caaagaatga tgaagaacgc
     1321 tatcaaagct tcatgaaaaa cttagatcgt tatctaagtg agcaaggcta taaacatact
     1381 cattttactg acgctgctgg ctatgactcc aatgctacaa catcagcgga agatttgaca
     1441 aaagtagctg caaagctact aaagaatgat aaatttgcag aaatagttgc caaggaaact
     1501 tatagagcaa aatcaattaa gggaaatgtt tataactgga atactacgaa tcaatttatg
     1561 caacaagata atagatactt tactgaaggt gtaaaaggtg taaaaactgg atcgtttgat
     1621 gggagataca atctatgtgt gtattataca tctagttctg ataaccacta tttacttgta
     1681 agttttggtt cagagagtga tagtaggcgt tatgtagatg ttcaaaatct attgcataat
     1741 ctgccttgaa atttcgtgcc tttaagcgcg tatctaaagt ttctttgaaa agtttgagaa
     1801 tgaagaaagt gagcagtcga aaaacggttg ctcacttttt tgccctaaaa tcgaaaaaat
     1861 ataaaaaagt cctttacaac acgtctcagg aaaaactgct aagtcaattc taatatcttg
     1921 tggagcaaga cttgcacctt ttgatataag agagcttaga gaactaatga gcgaagatga
     1981 actagaactc gataaaattg gagatagaaa aactgccttg tttgtaataa tatcagatac
     2041 agatgataca tttaattttg tagtttcaat aatgtattct caattattta atctactatg
     2101 tgataaggca gatgatgtgt atggtggaag acttccagtt catgttaggt gtctacttga
     2161 tgagtttgca aatatcggtt tgattcctaa atttgaaaaa ctaatagcga caattcgttc
     2221 aagagaaata tcggcaagta taatactgca agcacaatct caattaaaag caatttataa
     2281 agaccatgca gatacaatag ttggtaactg tgactctaca ctttttctag gaggaaaaga
     2341 aaaaacaaca gtaaaagaat tatcagaaac cttaggaaaa gaaaccatag acctatataa
     2401 cacatcagaa acaagatcaa atcaaaaatc ttttggtcta aattatcaaa agactggcaa
     2461 ggaacttatg agtcaagatg aaataactgt aatggatggt ggtaagtgta tttaccagct
     2521 aagaggagca agaccttttc tatctgataa atttgatatt acaaagcata agaattacaa
     2581 attgttggaa gattatgata agaaaaactt gtttgatgta gaagagtatt taaaacaaag
     2641 atagcgtaaa atattttgtg taaataactc gtccttctgc aaaataatgg gttactcagt
     2701 aaacattgaa gctaatgtat ccgtcacttg ttggaagcct ttatggcttc tatttaagaa
     2761 tttttgattg tatgtatcaa agatggatac taggaaacgc tctagtgatt cttcattttg
     2821 aaactgctct tttctgtggc tgtatttctt gatttgctta ttaaaggatt caatcaagtt
     2881 cgtagagtaa atagtcttgc gtatggctaa tggaaaatca tagaaagtga gcaggtaatc
     2941 gttcgaaagg atcgattcca ccatttttgg ataactggtt ttccattttt ccgcaaaagc
     3001 accacgtgct tccaatgccg cctttttaga tgacgcttga tacaccattt taaaatcatc
     3061 acagacttcc ttacgatcat cgacacgcac tttgtgactg atattacggg aaacgtgcac
     3121 acagcaatgt tgaaaacggg ctttggggta gaaccgctgc acggcccctg ccatcccctt
     3181 taagccatcc gtgatgaaaa ggaggacgtt tttcacgcca cgttcctgga ggtctattaa
     3241 aatctcttcc caaatcgtga tagattcagt tggtgcaatc gcatagctca acacttcctt
     3301 tgaaccgtcc gggcgaatgc caactgcgat atgaatggct tccttggcga cggttttccg
     3361 ctttaaaggg atataggttg cgtccatata aatagcggca taacggtcat gaagctcacg
     3421 ttccttaaat gaagtgactt cttccgtaaa tgatttagtc atattggaca tggtttgggg
     3481 cgtgtagtga tgcccataca ttttctcaat caagtctgcg atttccgaca tggtaatacc
     3541 ttttcggaag aggtgaatga cggtctcctc taaggtatca ttcgtccgtc tataagcagg
     3601 aacagtctgt tgcttgaact cgccgttgcg gtcgcgcgga atctggagat gaagttcccc
     3661 gtactcggtc ttgaccgtac ggtcatagga gccgttacga gaattacccg tgttaaaacc
     3721 aatgcgatcg tacttttcgt aatcgaggaa agccgttaac tccgttttga gaagcgtatt
     3781 gacagctttt tccaaatgga cacggaataa ttcattcaaa tcgcctttat tcgctagagt
     3841 ctttagaatt tctgtagtaa aatcgttcat atggaagtcc tcttttctgt gaatgtgttg
     3901 tggtaactct attctactga agggacttcc ttttttctat ttacacaaaa tattttacac
     3961 tctcggattg aaagaacgcg gtctttcagg agtaaagatg gtcatctcgg atgcgcatat
     4021 agggctcaca aaagctgtga aagaatacct tctttgtgcg agctagcaga gatgccaagc
     4081 acatctcctt cgcaacattt ccgacaaatt ctccaagaaa atctcaagcg atatcaaaga
     4141 agactttaaa gccatcttca aaacggcaag catcgagctg gctggagaac gtaaacaact
     4201 tattatggat aaatatgggg aagacaagaa agtcgcaact gcctgtaaaa tattggatga
     4261 gggcttcgaa gatgccatgc aagtaatggt tttaccagaa aatatccgtc aacgattgag
     4321 aacaacgaat gttcttgaaa gactaaatga agaagtacgc cgtcgtgaac gtgttgtccg
     4381 aattttccca aattgtgatt ctgttattcg tatcatcggt gctttattga tggaaaagga
     4441 tgacgaatgg acttctgcac ctagaaaata tcttgaattt gatcgtaacg agatttagca
     4501 cattaagccg ccaatagtat aatatactgg tgagaagtat caagagacgc ttcgcttttt
     4561 gctcttgaca cttcttacca gcatattgga ccgtagctag gctcaacaag gcaaaacatc
     4621 gtactttgag atttcctttt tacacaagat tttggacttg acacagcccc tatggttctt
     4681 accttgttta tgcttattta aatcttactt tattacagta ctttcaaact tcgttttgta
     4741 agccgggtat tttgatattt cataattatg tccctctttt tgaatatatt ctttgatatc
     4801 tgcttgttca tctacacttt ctttgcgtaa aatacgttcg atcattgagc agcagtgcat
     4861 gtaaagcaag aacaattgat tgtttgaaaa ttcgatcccc attagttttt cgagttcttt
     4921 gacggccttt tgtaccaatc tcaaaacctt cattgcatca aggatcgtta aattttctac
     4981 gatcttgtta atcgagaaat taaagtattg agcacaatta taggcgataa atggagcatt
     5041 tgattctatc acctgttgtt ccttggcgta ttcataaatt ttttctgcca aaaaggtttt
     5101 tcctgaaccg cttacaccca tcaataaaat cggtaatccg ttatgaggat acaagacagc
     5161 cgtcttgatt tgatcgatcg cttccttt
//
LOCUS       DASJUF010000150         5176 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.154, whole genome shotgun sequence.
ACCESSION   DASJUF010000150 DASJUF010000000
VERSION     DASJUF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.154"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..1098)
                     /locus_tag="U4B62_002662"
     CDS             complement(136..1098)
                     /locus_tag="U4B62_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2142763.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="U4B62_002663"
     CDS             complement(1100..2539)
                     /locus_tag="U4B62_002663"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN2142764.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="U4B62_002664"
     CDS             2724..4670
                     /locus_tag="U4B62_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN2142765.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="U4B62_002665"
     CDS             4934..>5176
                     /locus_tag="U4B62_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2142766.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DASJUF010000151         5085 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJUF010000151 DASJUF010000000
VERSION     DASJUF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.156"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="U4B62_002666"
     CDS             complement(<1..182)
                     /locus_tag="U4B62_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2142767.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="U4B62_002667"
     CDS             517..951
                     /locus_tag="U4B62_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2142768.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="U4B62_002668"
     CDS             974..1294
                     /locus_tag="U4B62_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142769.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="U4B62_002669"
     CDS             1435..1626
                     /locus_tag="U4B62_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142770.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="U4B62_002670"
     CDS             complement(1697..1954)
                     /locus_tag="U4B62_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN2142771.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="U4B62_002671"
     CDS             2112..2375
                     /locus_tag="U4B62_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142772.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="U4B62_002672"
     CDS             2399..2587
                     /locus_tag="U4B62_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142773.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="U4B62_002673"
     CDS             complement(2670..2855)
                     /locus_tag="U4B62_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142774.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="U4B62_002674"
     CDS             3070..3441
                     /locus_tag="U4B62_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2142775.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="U4B62_002675"
     tRNA            complement(3591..3663)
                     /locus_tag="U4B62_002675"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="U4B62_002676"
     CDS             complement(3709..4137)
                     /locus_tag="U4B62_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142776.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="U4B62_002677"
     CDS             complement(4197..4955)
                     /locus_tag="U4B62_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2142777.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1775 a          784 c          991 g         1535 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaa
//
LOCUS       DASJUF010000152         5023 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.157, whole genome shotgun sequence.
ACCESSION   DASJUF010000152 DASJUF010000000
VERSION     DASJUF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.157"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(489..863)
                     /locus_tag="U4B62_002678"
     CDS             complement(489..863)
                     /locus_tag="U4B62_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2142778.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(886..2286)
                     /locus_tag="U4B62_002679"
     CDS             complement(886..2286)
                     /locus_tag="U4B62_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEN2142779.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2290..2700)
                     /locus_tag="U4B62_002680"
     CDS             complement(2290..2700)
                     /locus_tag="U4B62_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2142780.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2718..3209)
                     /locus_tag="U4B62_002681"
     CDS             complement(2718..3209)
                     /locus_tag="U4B62_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2142781.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3219..4010)
                     /locus_tag="U4B62_002682"
     CDS             complement(3219..4010)
                     /locus_tag="U4B62_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2142782.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(4003..4776)
                     /locus_tag="U4B62_002683"
     CDS             complement(4003..4776)
                     /locus_tag="U4B62_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2142783.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1592 a         1020 c          673 g         1738 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt ttcccagaac ggtaattcca taatattttt atctaataat
      601 tcatatccta aatcttgaat tgaattgaat acctcatcta tgtcatccgt atctaatgct
      661 agatgatcaa tggctcctct acactcagta gttttatctt cccatatttc tattatcgtt
      721 ccattcaact cgaaaaagcc gactttactt cccacattca tttcttcatt aatcagacta
      781 aaccctaagg attcataaaa gatttttgaa gctccaaaat cttttgttgg aattccaata
      841 tgttgtaaac catttaattt cacttacatc atcccttcaa ttgttttaaa atccacattc
      901 ataatttatt gaatagtcta gtttatctac cttgattgct ccctccgtaa cagtcatact
      961 taatgctgtt ccgactttat catacattct agtacttaaa gcaacattat caatatataa
     1021 aacagctatc gtatcatcaa caattaactt tagttcatat tctttgccta tttccaaaaa
     1081 tagtggtcgt tgcagcccct tatcaaacat ttgaggccaa ggatagttcg gagtttttcc
     1141 gaaagtaagc tgattattaa tcaatgaaat acgaaattcg tatccttctt gagtatcact
     1201 atctacaaat aattttaaag caaactcctt tgttaacgat gtaatagtca aagttgtttc
     1261 aaatagaaaa taatcttcga ttttaggaac aattactctt tctttttttc caaattcttt
     1321 ctctaactta aaacttgaaa atggagtgat ttctttaaaa ttctcaatta ttttttcagg
     1381 cagtttaaca ccaagagttt tgtctgtctt ttgaataatt tgatgtggaa caagtgcacc
     1441 gccccaatcc cagttatttt gatcgttctc cccttctttt gttgccaccc agccagttag
     1501 aaaacggcca gtttcattag ttccgactgt tcttgctgca taataggctt ttccgtcgaa
     1561 agcttcgtct actggtgatt tccaatctga aaataatcct gctccgactc gatatttagt
     1621 ctttttatct tcacaatact ctgaaaaaag taaataccac gttccatcaa tttcaaatat
     1681 atcaggcatt tcaatcatgt taaattcatt attagcccag tagtctcctt tgaaattcca
     1741 atcttttaaa tcttcggatt caaaatagac caatctcccc gtcggctttt tcttacttgc
     1801 tggttttcta gctcctagta tcaaaacgta ttttccatga tcttgatcaa aaattgcaaa
     1861 aggatcgcgc caatcattct tatcatatcc ttcttgagga acaagttttg ctgcttcgcc
     1921 atcctttttc caatgaatta aatctttaga acttgcaatc attaacactt ctgaagtttt
     1981 tccatccaac ttagctttgc gattatgtcc agtatacaaa gctaaatact cattgattcc
     2041 ttggacaata cttccagcgt aaatgtattg atcaatgtca tcgttgccac cacgatgaat
     2101 tacttccccg tattcttcaa atttcacaaa atcagtggtt gtaactagtg accacccaaa
     2161 aggatcagtt aaaggtttag gatttctcgt atctcgttga tgaaatagat aaaaagttcc
     2221 atctttataa aaaggcatgc aatccccaaa ccaaaaatct ttaggcttgt aaaaaatttt
     2281 ttgcatttat cactcatcct cttcgttagt atgtctttgc atatctgcaa gacacgcaat
     2341 tgtattcata tttcttagat tttcgatgat atcatcgctt ttttttcctt gtaatctcaa
     2401 attgacagcc tcaatcaaaa ttcctaaatt catgccacca agaagttgaa acccattttg
     2461 aaatacttca cttcttttag caaattcgtt gaaaggtgtt ccaccaatca agtcagcaaa
     2521 taccataact tcttgatttt ttacttgaga aactattttt tcaatgtttt cacggaattc
     2581 ttcaatgcct gcatctccaa gtgttaccac atgacaattt aaattatcgc caaaaaacat
     2641 ttttaatgta tctacaaatc caatggcaat atttccatga gatgtaatta taaactccat
     2701 ccactcatcc tctgttctta ataaataaaa tcctttaaaa attcttttga atctctagga
     2761 gtttgtatca ctgtcacttc aactccatca ctaattagtt ctttaaaagc ctctctctct
     2821 tcttcagtaa tatttatcgt cggacggatg cgttctgttc cattccttct tgataaattt
     2881 cctacgttta cacttgttat tggtaaaccg ttatcaatca aaaatttaac atcgattgga
     2941 gaaactgcaa caataagaac tttttgtcct tcatatttac cagaaagaat atttttaact
     3001 gctacttccc ttgtaattat tgaagttcta acattaggtg gtgcagcgag gcgaactaca
     3061 gcttttttta cgtcatcatt ggctacttca ttattaataa ccataattct gttagcactc
     3121 aattgatttg tccattgtgt tgctacttgt ccatgaatca ttcgatcatc aatacgaaca
     3181 tgtataatac ctttcatttt ttcatccata ttcaacacct acttcaaaat atttaacgct
     3241 cttaacgcaa tagaaactac taagattaat agaattacat aaactggttt aacctttcgt
     3301 cctaacaaat aatacacaat tgctactaca catgcaggta ataaaccagg cataacttga
     3361 tcaaagattt catttccggt cattgttaag tcgccttgtt taaattcaaa gccaacattc
     3421 acttttacaa ctgtggcaat gagtgcaccg accaccatta accctaaaat tgatactgca
     3481 tcagtaatag actttaattt tgtttcaatt tgagttagta ggctcgcacc cgattctttc
     3541 ccagcaaggt ataaaggata agcagtaatc cttaataaaa ttgatgccag tatccacaaa
     3601 ataattccaa caaaactacc ttccaatgcc atattagctg caattgctcc aaaaatagtc
     3661 catggaataa ctacaaatac actatctcca attcccgcta aaggacccat tagacctgtt
     3721 ttcagagcac ttactgcttc caaagactta tttttttctt ctttttcaat tccaacattc
     3781 ataccaataa tgtaaggcgc tgtatacggc gtagtgttaa aaaattgtag gtgagctaaa
     3841 acggattgtt tcattccttc aggatcatct ttatatgtgt ccttgataaa aggaagcata
     3901 gaatacgcat aacccaaccc ttccattttt tcataattta atgaagttgc accattgaga
     3961 atccaccgga aaattacttg tttgtcgatt tttgtcagtt tcttattcat cttcgtcaaa
     4021 acctcctgtt gcagctggtg ctacttgagt attatttgat ctatttttat atacgattag
     4081 tgcgattgca gcgccaagta acgaaatccc taaaattggt atttggaaat aaactgccaa
     4141 aacgaaacca actaacaaat aggcaaagta atcttttgta ggtaaatatt taagtagcat
     4201 tcctatccca acagcaggta atagtccccc tgctaccttt aaaccaccgg taacccattc
     4261 aggaatataa ttgattgcac ttcccactaa cgttgggcct aatatcacag ttaacaaaac
     4321 aggaattcca gatacaaagc ttgtattaat tgatgccaaa tattgcatcc actctaattt
     4381 ttgataatct tctttttgtg cgaatttttc tgccttttgt tgaaaaaaaa tgtttgttga
     4441 ccactttaga acatctagtt gtacaattaa cattgctacc ggtattgcta aagttattcc
     4501 tatagaagca tcctggttag ttgagattgc taaataagta ccgatcaatg ctgctgtttg
     4561 ataatccggt actgatgcac caccaaaact agtaatacct agtgtcatta attgcaaagt
     4621 cccaccgata taaagtccag tttgaatatc tcccataata agtccggtca agaaacctgc
     4681 aacgacaggt tgggaggttc cgattttggg cccttcctta tcccagttaa tgaaaaatcc
     4741 atacaacaca actaaaaatg tttgccaaaa cgccattttg ttctctcctt tcttcttata
     4801 cttttatata agcaattact gtgccaacta acattatcca ataaagcggg aaaatacagt
     4861 aaatattaat taaatgagcg tctaaatata ctttggacac cctttcattt ttttagacgc
     4921 tagtatgcta tttagaaaat agtctgctca aaaattgtat agattaactc atactctcct
     4981 ttgggtattt caatacagta aatatctttg atttctgaaa aaa
//
LOCUS       DASJUF010000153         4978 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.158, whole genome shotgun sequence.
ACCESSION   DASJUF010000153 DASJUF010000000
VERSION     DASJUF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.158"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..971)
                     /locus_tag="U4B62_002684"
     CDS             complement(<1..971)
                     /locus_tag="U4B62_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142784.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADG"
     gene            complement(1179..2105)
                     /gene="glsA"
                     /locus_tag="U4B62_002685"
     CDS             complement(1179..2105)
                     /gene="glsA"
                     /locus_tag="U4B62_002685"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN2142785.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(2128..3555)
                     /locus_tag="U4B62_002686"
     CDS             complement(2128..3555)
                     /locus_tag="U4B62_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2142786.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3586..4800)
                     /locus_tag="U4B62_002687"
     CDS             complement(3586..4800)
                     /locus_tag="U4B62_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN2142787.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1564 a         1047 c          853 g         1514 t
ORIGIN      
        1 ccatctgccc acaacgtacg gacaaattgt aaaaaggatt gcttgaaaat aaaattttct
       61 gcatcgtcga tttcagcgat actaataatc tgtaaaatca attcattcag tgataggtca
      121 ttgagaacaa aatttttata aacaacttcg aaatcttttc ttaacagctc catgttctca
      181 ttggtataat ttctactgtt gaacaaagaa aaaatataat agacatcttc ttcttgtaag
      241 tctagaattc ctttttgctc ttccaatcct tgacagacta attgaaataa cggcttcgtt
      301 tctgcgcttt tcttatcttc ttctttcagt ccaaggggaa atgcctttct tccgattgcg
      361 atctctattc ctcgtatgat aaaggacttt tcatcttccg aatatcttct tttaaaaaaa
      421 tgagtctcta cataatcaat caactcgtta caagcttctt ctacatacgg atttttattt
      481 tcatagcctt cccaattgac ataacgtact aaagctagca agatgttccg cgagtcaaat
      541 tctgggataa tgatttcttt atttttcaaa taatcatttt ctttaaaaaa atccaatacg
      601 atttttttga ttgtatatac tttgctcaaa gaaataaatt caagatcagc cagttctgta
      661 ctcgtgaata taccattaaa ataataactt aaaaacttta aaaaataaga ttgaccgtac
      721 agaaactgtg tgagctcaga aagagcaata ccatgtttga atacgagttt atacttatgc
      781 ttctctctta cttgttcttt tatacaatat tctagacact cggctagact ttctagatcg
      841 tgaaggatcg ttggtgtagt cacccttaga gaaatagctg tttgttcgat gtctatttct
      901 tggaattcaa ataattgttc acacagatac acttttctaa atatatttcg ctcgatgtaa
      961 ttttccagca ttcctgatac acgcctccag tttttcttat gattttattt aatcatatct
     1021 gtaaatcgta tttttgtttc ctttttattt agaaaagccc tttcaaaaac caataaaaaa
     1081 agaaactggg acaaaagtca aacaagctca caatttcagc ctcattgtag gtagctttga
     1141 gccgtttgga cttttacccc agcctctttc aagaatggtt attcaaagat gtcagcgtct
     1201 agttctttga ctacatcttt caacaattgg ataccagctg cgctgttacc aaatggatct
     1261 agtgccgggc tgaagacacc tatgccgtaa cgatttggca cagctgccat caacccgccg
     1321 cctacaccgc tttttgcagg tacaccaaca tggacggaaa actctcctga ttcatcatac
     1381 aaaccagcag ttgtcataat tgatttgacg attgtagcac tttcttcagt aatgatttgt
     1441 ttgccattcc aaggagcaat tcctttattt gccagcacag cagctaattt tgctatgcct
     1501 gtagcggtga cattgaggga gcattgtctg aaataagcat cgagtatctc cggtacgtct
     1561 ccttcgatca tttgattacc tttcatatag taagcaagag agcgattgat atcacctgtt
     1621 cttgattcag aatgatagat ttcttcattc aatgtgatat taggatcatc acagatctct
     1681 ttcataaatg acaagattcg ctcaaatttt tcatctgcat ctttaccggc aatcaacgag
     1741 gttgttgcaa tcgctcctgc attaacaaaa ggattcattg gatgtttgcg atgattgatt
     1801 tccatattca tgatagaatt gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag
     1861 actttttcta ttccgttgtc tttgattgct aataacaata cgggaacttt tgacatgctt
     1921 tcgatggcaa agcgcacatc ggcgtctcct ccctcaatca gttgttttgt atccaaatca
     1981 taaatcgcca tacctaattg tttgggatcc acttcagcta atgctggaat gtaatccgcc
     2041 acttgtcctg tttgataata gtggaaattt tttccaatca attgttctaa ttcttttact
     2101 tccatttgaa taaccagctt tcatttttta tttttctgca tgctggaatt tatgacggca
     2161 tgcgtagatc gtaaatggaa taactaagac gataagataa gaaacaatca gaatcgtttc
     2221 ataaatccca tgctgtccag ctgcgatact agcaggtgga acaaatgaga tacaaaaagc
     2281 aaaaagtgaa gtcaaaagac ctaaacaagc aacgattact tttacaactt ttccgcctgg
     2341 aacctgatag gctctttcaa gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa
     2401 aaacataaag tacaacatca ggtagattac aacagtcaat gacatcgctg caagaaatga
     2461 aagattattc cccccgccgc ttaatgtaaa gacggctgcc cagattgttg caacgatccc
     2521 atttgcgatc atcaaatgag caggaacacc gtgtgtgttt tcttttgcaa aatattcagg
     2581 aagtaatcct tcttctgctg ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc
     2641 aatttctccg atgacaccta aacaaacaag aatcgcaaga attttcgcaa tccatgcaag
     2701 atttggatca atgtgtctta ataggaattc taacgattga atcacccctg tattaagaga
     2761 caaaccatgt tgaggtactg tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat
     2821 tgccagaata accaatacaa acattgctaa tggataatcc tttttagggt tttccatttc
     2881 atttgcatga ctggcagatg tttctacgcc catatatgct aaaataaagg aaacaaaaac
     2941 aaccagcgta ggcaatttcg aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg
     3001 tagtggagcg cctgaagcga cataatggat ccctaagcca aacaagacta atgctggcaa
     3061 caagatacca gctacaaaac cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa
     3121 ctgtgagagt aaaatagccc agaaaataat caataggatt gccaatttca gatatttatt
     3181 gttttgtata gcaccaatac ccgttgcata ggataatgca ccagttaaga aatacagcat
     3241 ggtcaagtag ccaactgtta tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg
     3301 ttttcccaat gtgcgactga cccaagtata gacaccgcct ttttcccatc ctttcaccgt
     3361 tgccatctct gctgcgcaca atgccacagg gataaaccat agcaaaccgc ctaataacaa
     3421 gaaaaatact aagctgaagc ctgaggtcgc gaatgtggga tattcatata atgaaacgac
     3481 cattgagatg gtcatagcca aaaagccaaa gaacgttaat ttttttgcag gaattttcgt
     3541 tttgctttgt tgcatgaatc ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt
     3601 gccaaagaag aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt
     3661 aaagtgcatc gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat
     3721 gatgacacca atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg
     3781 gctgttagat gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac
     3841 accccacaca tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg
     3901 acagacgata cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc
     3961 acaaggcgta atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa
     4021 gccatttcct tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat
     4081 gaatgttgaa ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt
     4141 aaaaccgaac cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct
     4201 atgctgactt ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa
     4261 agagccgaat ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat
     4321 ctgatccaaa aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt
     4381 ccaaaaaata acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt
     4441 catcaaagga acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg
     4501 aatcgttgca ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc
     4561 tgccgggttt ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa
     4621 tacccacata atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat
     4681 attattcttc cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac
     4741 cattgctgta cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat
     4801 ttttttcgcc tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc
     4861 tgtttttttg aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt
     4921 gttgtgctct ttttcgtttt tcacacaaat aaagacaaaa aataaacggt cttctttt
//
LOCUS       DASJUF010000154         4947 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.159, whole genome shotgun sequence.
ACCESSION   DASJUF010000154 DASJUF010000000
VERSION     DASJUF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.159"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..1771
                     /locus_tag="U4B62_002688"
     CDS             128..1771
                     /locus_tag="U4B62_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2142788.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(1844..2389)
                     /locus_tag="U4B62_002689"
     CDS             complement(1844..2389)
                     /locus_tag="U4B62_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN2142789.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(2412..2897)
                     /locus_tag="U4B62_002690"
     CDS             complement(2412..2897)
                     /locus_tag="U4B62_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2142790.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(2887..3699)
                     /locus_tag="U4B62_002691"
     CDS             complement(2887..3699)
                     /locus_tag="U4B62_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN2142791.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(3838..>4947)
                     /locus_tag="U4B62_002692"
     CDS             complement(3838..>4947)
                     /locus_tag="U4B62_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN2142792.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         1593 a          977 c          946 g         1431 t
ORIGIN      
        1 aaaataaagc tcaacgaacg cacttagcag attcgttgag cttttcgttt ctagttaccg
       61 gtaacaagga tacttctaaa atagaatcgg ttataatcaa gagggaccgt taaaggagtg
      121 aaacgatatg atacaagaac aaaaatggtg gaaagaagca gtaggctatc aaatctatcc
      181 tgctagtttc aaagatagca atggcgatgg tcaaggagac atcaatggga tccgtgaaaa
      241 acttaattat ttgaaagatc ttggtatcgg ctttatctgg atcaatccga tctatcaatc
      301 tccatttgtc gataatggtt atgacgtaag tgactatcaa gatatagaag agagattcgg
      361 aacgatggaa gattttgatt tattgctaaa agaagcacat gaattaggga tcaaagtgat
      421 tatggatctt gtcatcaacc atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa
      481 atctaaagaa agcccgtatc gcgattatta catttgggtc gacggggtag atggcaaaga
      541 accgaataat tggacatcga ttttcggcgg ttctgcttgg gagtattctc atgaaactgg
      601 acagtattat ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa
      661 attgaaagaa gaactattca atatgatccg atggtggttg gataaaggga tagatggatt
      721 tcgtttagat gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa
      781 ccctttctca ccctttcaaa atgtctcagg aatcgaagaa catttgaccg atttgaaaca
      841 tgtttttgaa gagtacgata tcatgactgt cggagaagca agtggtgtca ctgcagaaga
      901 aggaccacaa tgggtaggga aagacggtta tttcgatatg atattcgaat ttgatcatat
      961 ccatatctgg cagcaagaaa aagaagggca attagatgtc cttaaattga aacttgcatt
     1021 aagcgcttgg caaacttcat tggatggtat cggatggaac gctttatata tggaaaacca
     1081 tgatgttcct cgtgccgttt cagtgtttgg agatactcgt cccgattttt gggctatgtc
     1141 tgcaaaagca atcgctatga tgtatttctt tttgcaaggt acgccattta tttatcaagg
     1201 acaggaaatc ggaatgacga atatgccctt tgaatcgatc gatcaagttg atgcagtaga
     1261 ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag acaagagaag aagcattgga
     1321 tatcattcgt gaaacaacga gagacaacag caggacaccc atgcaatgga cttcggaaca
     1381 atatgctggt ttttctacac atgaaccttg gctgatcact aatccaaata ccaaaacgat
     1441 caatgtagaa caacaagaat atgaaccaga gtcggtcctg caattctata agaacatgat
     1501 ccgcattaga caaaccaata aaggtctgat atacggttct tataaagaat atcttcatga
     1561 acatccacaa ctttatgtct atgaaagata tttagaagat gaacaatatc ttattatggt
     1621 aaacttaaca gaatcacttg ctgactatga acttccaaaa gaagcagatc agtcctggac
     1681 attactcttg tcaaattcct ctagcggaga atttgaagct aaaggcatac tagcacctta
     1741 tgaagctcgg ttatataaaa caaacaagta aaacttcctc gttaaagttc attagttgga
     1801 ctttgcgagg aagtttttca gattgctctg tcaattactc atttcaataa ttcgctctag
     1861 gaccaccgat atatttagga cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt
     1921 tcaaacgagg tctgacaggt acttggacat gtttgatatg cataccgatt gatgtgtcac
     1981 cgatatcgat accggcttgt ccaactacgt gttccacttc gacaggatca ttgaactggt
     2041 caaaagcagc aacagaacaa gcacctccag catgtagtgc aggaacaaca gagacaattt
     2101 cgaaattttt tgcttctgcc actgttcgtt ctactactaa tgctcggttc aaatgttcac
     2161 aaccttggac agctagcgca atttctttag gatccaacaa ctcttttaac gtacggatga
     2221 tccattgacc aacttccgca ctggaatttt ttccaatatg tcctccaacg acttcgcttg
     2281 tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc aatatgtcat
     2341 taactatttc agtcaattgg tcttttaatt ctttttctga caacttcatt ttctaaaacc
     2401 ttctttttca attaatattg cttccgtgct tgaatacgtt gaagacttgc agttatagga
     2461 aaggcaagtc ccattccaat cagattttgg aagatgtttc ctggtataga agccactcca
     2521 gccggccaat catataaaat ccaaccggcg ataccatagc cgatgatcat aactaagctt
     2581 cctaataaaa gccctaatcc ccacatacga gtagaagatt tgctgaaaga accagcaaca
     2641 tatccttgta acccgtggat cactaatgaa aaaactgccc actgcggata tcctgaaatc
     2701 aaatcgatca atagaccact ggaagcacca acaactaatc cacccgttgg accataaaga
     2761 aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa atccattcgt agccggtaca
     2821 gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa tcagcatagc atagatcgtt
     2881 aattgcttag ttttcatttc tcattcctcc tttttgaata ccgccagacc agacacttcc
     2941 ggtatgatcg ttcatcagca cgccattaga aatacaatgg gcaacaaatt ttttactttc
     3001 tgctactgct ttggtaaaat ctgcccctaa cacaagattc gctgttattg cagaagaaaa
     3061 ggcacaaccg gccccgttta ctgttgtagt gtcgattttt ttatgagaaa atacctcata
     3121 atgatcaatt gaatagagaa gatccacagc ttcatctcca gccatccttg tgccgccttt
     3181 gatcacaatg ttttttgttc ctgaagcagt gagttcttta gcgagatgga tcatttctgt
     3241 taacgaagag ggaagagctt gaccagaaag taaagcagct tctgccaaat taggcgtaat
     3301 catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa atatacaatt gattgatcac
     3361 agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg attgggatat tttgttttgt
     3421 ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc caattttgat
     3481 accatctaat gaagtcattt tatcaatcga aactaactgt cgtttaagca attccaaaga
     3541 tatagattcc agcataaatc gctcattaat atctagtgcc cctatagaag tcaaggcgct
     3601 tagtccgaat agaccaaaat tctcaaaagt ttttaaatct gtttggattc ctccaccagc
     3661 aaatggatca gaaccgccaa ttgtcaagat tcttttcata acattttcct ccatgaataa
     3721 gagtataacg aaattttcaa aaaagaaaaa cagccaattg gcatattttt atctcatcca
     3781 atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc tttctttcct taaatagtta
     3841 ggacttttgt gattttaatt ctttctcgat ttgaagccgt tgcactttca ttgtagctgt
     3901 tcgcgggatt gcatcaaaat cacggatgat agggacgttc aagcgaggaa gttcatggac
     3961 ttgctcccac catcgattcc aatccatttc tttatcagga gctaaagcaa taatcggttg
     4021 tggagaatga tttttatccc ttacaatcac tacttctgcc aaaaaatcta acgaatctaa
     4081 taaaaagtct tcaatagcta atgtactatt gatcgtttcg atcaaatcca cttgccgatc
     4141 tttcaaaaat aggtggccat gcttatccat aaaaccgtaa tctccgctat cccaccaatc
     4201 gccataaaca ttttcctgga accgtgcatc ttccttgtaa taagtcaaag cccgtccttt
     4261 agacagtaat tgtatatgac catctgtgtt ttcaggtagg acattcccaa ctgaatctgt
     4321 aatccgagcg cttgtcatgt cttccagtcc aacgcccata tctcttgcat cacttgtttt
     4381 caaactctct agtgtatgag ccttaagaat cattggtccg cattcacttt ggccatatac
     4441 ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt
     4501 gtttatagca tcaaaagtgg agtgatagta tcggataccc gaaaaagctt ctggatgctc
     4561 cttagcagta aaacgccact gaacaaagtt attcggatgg gtctctagtg caattggccg
     4621 gtgagcttcc aacatatgaa caacttttga actttgtgcg tttgctaaag gcatcatagg
     4681 aaatcccatg gacatcaaag aagagatacc aatattgaat ctggaatgca ctggagagat
     4741 gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa actgtttttt gccatttcgt
     4801 ccgccatccc atagaatgag cagaatgaca aataagttta gggataccag ttgtaccaga
     4861 agtatgggtc atataagaaa tttcattttt gtctaaaaat gattgagaaa cagacaaagc
     4921 aggttgttcc attaaataaa gaatagg
//
LOCUS       DASJUF010000155         4941 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJUF010000155 DASJUF010000000
VERSION     DASJUF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.160"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..832)
                     /locus_tag="U4B62_002693"
     CDS             complement(<1..832)
                     /locus_tag="U4B62_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2142793.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1643)
                     /locus_tag="U4B62_002694"
     CDS             complement(846..1643)
                     /locus_tag="U4B62_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2142794.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1676..1960)
                     /locus_tag="U4B62_002695"
     CDS             complement(1676..1960)
                     /locus_tag="U4B62_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN2142795.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTLSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1957..2958)
                     /locus_tag="U4B62_002696"
     CDS             complement(1957..2958)
                     /locus_tag="U4B62_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN2142796.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELFKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="U4B62_002697"
     CDS             complement(2960..4027)
                     /gene="gnd"
                     /locus_tag="U4B62_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEN2142797.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            4026..4874
                     /locus_tag="U4B62_002698"
     CDS             4026..4874
                     /locus_tag="U4B62_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2142798.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         1493 a         1080 c          799 g         1569 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgcca catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat ctgttataaa aaaagcagct
     1501 tgtctgtaaa agtgttctgt tttcccttct gcttgtccat tctccttata catgacagca
     1561 agaggtgtcc atggagcaca attactagct aaagtaggga tgactccgaa atcgattctt
     1621 gcaagattgg cactataacc cactagatcg gtcagttttc ctccgccaac gccaactacg
     1681 aaatcgattt catttttttg atgattgtag ctattcgtct ggcttcagca taactacatt
     1741 ctccagaata acgatgataa aaaaattgaa tcgtttcgtc attcaaaaat actaatttag
     1801 gtagcgcctt ttcccatgat aacgttccgt gggtgaccag tactcttttc gcgccgtatg
     1861 atttcaagag atcagaaact tttgaaatac caccttcttc atataaataa aattgagggc
     1921 ctgttttcac ttttaaaccg ttcaccattt tcttcatcat ccttttatat agttgctctt
     1981 tgtctggaca gaacgttcag gaagaaggtg ctgactgatc tgttcgacat ctctgacaca
     2041 tttttcaccc attgcttcta aacattccct tgtgtaggcc gatatatgtg gcgtaatcac
     2101 tacttgttca aacgctaaat acggatgatc ttgacgtccc ggttcttctt ctaaaacatc
     2161 tgtggcatat cctgcgatct ttccgctttt cagaccatta atcatcgctt cttcatcaac
     2221 tagagcgcct cttgcactat tagatagata aacattttct ttcatctgat cgacttcttt
     2281 atcagaaatc atgtgatagt tctcttctgt caaattcgca cataaacaaa tgatatctga
     2341 ttgttccagt aattcttgaa gttctacttt ttttgcacca aagctttcga tgtataaatc
     2401 tgatttgtaa ggatcataag ctagaacatc acagcgaaaa ccgttacgca aaatttcgat
     2461 tactccgctt ccggtatttc ctgcaccaat cacaccaact gttttgttaa acagcgtcct
     2521 accgacgaac tccgcccgat cttcccatcg atcgtccttg actcttgctt gtgcttgtgt
     2581 cgtgcgtcga agcatggaca acaaattagt tatattattt tctgctacag catttcgttc
     2641 aactaaagca ggtatgatag atacgattgt ctggtgttct tttgctgctt ttaaatcaat
     2701 attgttgtaa ccaataccat gtcgggaaat caatagcagt tcatctttgt tttcaaaaaa
     2761 atctcgtcga aaaaatggcg taacacttgc aataatgata ttgaatcctt taaataactc
     2821 cgctagttct tttcctggaa tctcttgatc aacagtaaag aaatgaacgt ctccaactga
     2881 ctttaatctt tccaagtggt gtggaaagac tcttccgaaa ctgctggaat taacgattgc
     2941 tatatgataa tctggcattt tactcaccct tttcaactat ttcatgtccg ccaaacccat
     3001 ttcttagtgc tgaaactact tttgctgaaa aactatcttc ttcctgtgaa agattcctca
     3061 tgaacaaact agttgcaatt actggaactg gaacagccat ttctagcgct gtttcaaccg
     3121 tccattttgc ttcacctgat gcagaaacga ttccccgata attacttaaa ttgggatgtt
     3181 cgtttaactg atcttccatg atttccatca gccagccgcg aatcaccgaa ccatgattcc
     3241 aaacacgtgc gacttgttcc aagtcaaaag catactcact tttttgtaca agttggaatc
     3301 cttcaccgat tgcttgcatc attccatatt cgattccatt atggatcatt ttcaagaagt
     3361 gtccgcttcc ggatgcacca gcatacagat atcctctttc aattgctaca tctttgaata
     3421 attgttccac ggatagaaaa gtttctttat ctcctccaat cattaagcag gcttgatgtc
     3481 gtgctccttc gattcctcca gaagtcccac aatccaagta accgatttca tttttctgaa
     3541 actcttctgc tcgacggatt gaatctttat aataagagtt tccgccttca ataataatgt
     3601 ctcctggtcc gagttttgga aaaagttttc ttaacatgtt ttcggttatc tctccagcag
     3661 gaagcattaa ccagatgatt tttttctgct caaaagtagc tagaaattca gagatctctt
     3721 gataagcagg aatgtcattt tctcttgcct gactaacagc tgtacttgaa atgtcgagtc
     3781 cttgtacttt gtgatggtgc tcttttaagt ttaaagctaa attcaatccc atttttccca
     3841 aaccaattac accaatttcc atgatagcgc ctccatttct tataccctca ttatacaaaa
     3901 aaattttttt gtaaatagat tttacaaaaa tattttttgt ttgatttttt cctactctcc
     3961 ttgtatcttt aacattaatg agaacggaaa aaatgataat cttattatga aaatgaggaa
     4021 ttctcatgag cgtctccata agactacaaa tcaaaacaat gtacaacgac ttcagcccaa
     4081 aagaacaggc tatcgccgat tatattttag aaaatccaag caaagtaagt catagtccta
     4141 ttagcaattt agcaagtgaa ctgggcattg ctgactctac tttttttcaa tttacaaaaa
     4201 aacttggtta taatggattt aaagatttta aaatggccat gttgatccaa gaaaatgatc
     4261 tatctgctat ttctatccac gaaaatattc agaaaacaga tactgagctc actatggcgc
     4321 aaaaggtgtt cgagtctagt atttcaactt taaatgacac aaaaaagttg ttacaacaaa
     4381 aggatctaaa aaaagcggct actattatca atgaatctaa acgtgtctat tttttcggtg
     4441 ttggcggttc agagattgta gcaactgacg cttatcataa atttttgcgt tcccctatcg
     4501 caacttctca cagcacggat taccatattc aattgatgga agcttcttta ttgacggaaa
     4561 aagactgtgc tgttttgatt tctcacacag ggcaatcaaa agaaacaatc catattgctg
     4621 aaaccgtaag aaaaactgga gcaaaaacaa tcgttattac tagccaagct aattcttccc
     4681 ttgctaaact tggcgatgtc gtttttattt ctatttctga agaaacagaa ttccgatctg
     4741 aagctttagc ttcccgtatt tctcaactaa gcattttgga ctctctatat gtgattttga
     4801 tgttttacaa cgaaaaagaa tcacgtcgtt ctatcgctaa agttcgacat actatttctg
     4861 aaatcaaaga atagcaccat aacagaaaaa gcgaaaaatc cataaaacaa aaaaagccac
     4921 taccaaatat ttggtagtgg c
//
LOCUS       DASJUF010000156         4933 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.161, whole genome shotgun sequence.
ACCESSION   DASJUF010000156 DASJUF010000000
VERSION     DASJUF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.161"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..337)
                     /locus_tag="U4B62_002699"
     CDS             complement(<1..337)
                     /locus_tag="U4B62_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142799.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFG"
     gene            complement(348..1016)
                     /locus_tag="U4B62_002700"
     CDS             complement(348..1016)
                     /locus_tag="U4B62_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2142800.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1146..1730)
                     /locus_tag="U4B62_002701"
     CDS             complement(1146..1730)
                     /locus_tag="U4B62_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2142801.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2061..2294)
                     /locus_tag="U4B62_002702"
     CDS             complement(2061..2294)
                     /locus_tag="U4B62_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN2142802.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2309..3259)
                     /locus_tag="U4B62_002703"
     CDS             complement(2309..3259)
                     /locus_tag="U4B62_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN2142803.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3256..4098)
                     /locus_tag="U4B62_002704"
     CDS             complement(3256..4098)
                     /locus_tag="U4B62_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2142804.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4100..4819)
                     /locus_tag="U4B62_002705"
     CDS             complement(4100..4819)
                     /locus_tag="U4B62_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2142805.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1678 a          893 c          611 g         1751 t
ORIGIN      
        1 atccaaagtt ttccatcgaa aatactaact cactcgttgt atttgttgta aagaattgtc
       61 ctctaggatc ttgacttacg tagccaatat tttttgatat actttcttgt gttaaggatt
      121 gtagcgagtt attttcaaat aaaatttccc caaccaattt tccgtcataa agtcttggaa
      181 taagtccatt cattaacttt aatagtgtgg atttcccact cccagaagaa ccagtaatca
      241 atgtaagact gccttttttt attgaaaaat taaggtcaac aagcgcttgt tccttaacat
      301 ccttatatga aaaagaaaca ttgttaaatt caaacaattc tttcacctca aatcaaaata
      361 cttaagaaaa aaatagtaac ggaaaacagg atgatatagt ctaataagct catgtttgtt
      421 ttatagtaag aacttctttt cacaggagat tctatcccct ttgttattgc attagcggtc
      481 atttcttcag caatcatagt tgcacgtata agaacgggaa ctaacagaaa ttccaaatat
      541 tgcagaggct gaaaaaaaga caagcgaatg tttctcattt ttaaagattc tttaatcatt
      601 cttatttcag aacgataagt cggaaagaat ctgatcacta ctgtaatact taacattatt
      661 tttttcggta aatgtatttt ttctaaagag gataataatt cattgctagt atagacacta
      721 accaacaacc atgagatcat tacgaccggc aacattcgaa atacaatata taaggatgtc
      781 gcaaaaaata tagtaaaaaa ctctatcttg atagcatcaa taattttcat tactaaaaac
      841 agaactgaat aaatcaagat gaaataaata catgtcttta ttttacgaaa catcaatact
      901 aaaactgtag caataatgaa acaccccact tgaaatatat ttccctttcc taaaaaaata
      961 atgaccggaa aaaaaatatt taaagtaatc aaggttaacg gattgatttt tgacactctt
     1021 tcacctccaa aaaataagat cgcactgtta tcttaagctg ataaaaatgc accgttattc
     1081 agataacagt gcgatcttat caaaaagcat aactgagaac aattctcaat gcaagctttt
     1141 tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt
     1201 aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag
     1261 aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg attgatcatt
     1321 ccgatagtct ttaacttatc tagacaacag actgtctttt tcgtcacaaa tgatgtaatt
     1381 ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaatcaa atcgaatata
     1441 cgtttattct caaaaatata attaataaat ggatctaatt cattgaagtt ctctctccaa
     1501 cttttctcta tatctatatt ttctaagttt aagaggtatt tggtaaactc ttcattatac
     1561 atattaataa gcgcattaat atgcggctga ataatttctt caaataacgc ttccttagaa
     1621 gtaaaatgtt tataaaaagc acccgtagtt acgcctgcgg tcaggcacag ttgccttatc
     1681 ttagtttgct gaaatccttc tttttcaaaa tggcttagtc cactttctat taacttttgg
     1741 tgagttataa tataatctct ggtttctatg ggtacttcct cctaataaga tgaattaact
     1801 ttattatctt acacaggact tgaagtttca atcgttaacg tttattactg aactcattcc
     1861 ttagagctaa actaatgaga aagagcagaa taactgatac aaaaagcaat cctacaagaa
     1921 ttaatagact catctaaatc attcaccttt tctaaaataa tccgagaaag atttaaaaaa
     1981 attatatcca gctattagga taagtcctat cataatcact ccaatagcca ttttcttatc
     2041 ctccctaaaa ataaagtctt ttacttaaaa ccttttttgt aatcgtcaat aaattttttc
     2101 cctttatcag ttaattgaaa tcctatatct aaatagtcct catatctaag tttagccagc
     2161 atctcactta cagatgctgg actaacactt agaacttttg ctaaatcatt attttttaat
     2221 ttcttttttc tttttgataa ttcctgaatt gctagtaaat aaattagtat agtagtcgtt
     2281 ttaatttgta ccatacctac cctccaaatt atttcgaaag gccttcagca attttatcca
     2341 ggttccattt catcatcgca tagtaactat caccatcttg tcctttttct gcaattgaat
     2401 cagtaaaaat cgttgaatag ataggaatat tggtatcttt tgatactgtt ttcattggtc
     2461 tatcatccac actgctttct acgaataagg agggaacttt tgttgtgcgt aatttttcaa
     2521 ctaagtgttt tatttgatct ggtgttcctt cttcttcagt gttgatttcc caaatgtaag
     2581 cagaaggcac attatacgct ttcgagaaat atttaaagca tccttcactt gtaacaatca
     2641 tttttttatc attcggaatt gaagcaaatt tagatttagc ttctttgtct agagaatcca
     2701 atttttcaat atacttatct agattttctt tatagaattt tttattatca ggatcttttt
     2761 ccgctaactg tttttcaata tttttagcgt aaataatacc gttttctaaa tttaaccaag
     2821 catgaggatc ttccttccct ttctcactct gaccctctaa gtaaataaca tctatgccat
     2881 cacttgctgc aaaatagtct ttgttttcct ctttgttcgc atttttaact aattttgtaa
     2941 accaagcatt tcctccagtt tccaagttaa caccgttata aaaaatcaaa tctgcttttg
     3001 aagttttttg aacatcttca ggcaaaggtt catattcgtg gggatctttc ccaataggta
     3061 cgatactgtg taaatcaatt ttatcttttg ctatattttc ggtaatatct gctaaaatcg
     3121 aattagtaac tactactttt aatttttcgt tcgaagccac tttatctttg tctttactat
     3181 ttattgaaca tgccccgaat ataaaaagag caatcaaaat ccccagactt cccagtactg
     3241 ctttccattt tctcatcata acgatctctc cttctttctt aaaaattttt gttttggtga
     3301 gattagaaaa ctaattacaa ataagaatgc cgctgttaaa acaatacttg atcctgctgc
     3361 tatgttaaaa ctataaccaa taaacaatcc aagcaatgat gaacttgctc cgactgttgc
     3421 agaaataaat atcatagttt tcaagctatt tgcatataaa tatgctgtag ctgctggtgt
     3481 aattaacatt gcaacaatta atatagttcc cacactttgc atagctgtta ctgctactaa
     3541 agttaataga accatcaata gataatgata aaaactgaca ttcatcccta ttgcttttgc
     3601 cattaatgga tcaaaagaag tgattaataa ttctttaaaa aatagaacta ctataaccaa
     3661 cactaaaacc gaaacgccaa ttgtaatcca cttatcaata tcttgtacag ctaaaatatt
     3721 tccaaataaa atatgaaata aatccgttga gctatttgca actcctataa ggataactcc
     3781 tagtgctaaa aaagaactga aagtaatacc aattgctgta tctcctttta ttacactatt
     3841 gctttttata aaagtaataa tgatagaagc taatagacca aatacaatcg caccaataaa
     3901 aaaattaata cctaaaatat aagaaattgc tactcctggc aaaacagcat gagaaatagc
     3961 atcacccata agagacattc ctcttaaaat aataaaacaa cctactgttc cagaaacaat
     4021 ccctataact acagatgtaa ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt
     4081 aataaaattt tgaagcatat tattccccct ctccaataaa tatagtatct ccataggctt
     4141 tttttaaatt ttcttcatta aatacagatt ctacacttcc gtgagcaatt agatttcgat
     4201 tcaaaagaat aatattatca aaatacttct ttaccttgct taaatcatga tgcacaatta
     4261 gcactgtttt tccctgatgt ttcaattttt ttaacgtatc cataattatt cgttcactaa
     4321 ctaagtctat ccctacaaat ggttcatcta aaaatatgaa atccgcattt tgagcaagac
     4381 atcttgctaa aagtacacgt tgaaattgtc ctccagataa ttctcctatt tgatgattag
     4441 aatattttcc catatcgacc ttttccaaag cctttgaaac tctttgccat tctgcatttt
     4501 taattctttg aaaaactttc attccggcat atgttcccat agatacacat tcttttactt
     4561 ttattggaaa agtaaaatca atatcagctt tttgctctac atatgcaatc ttcaccagtt
     4621 ttttattgat atcttcatta ttaatggaga cctctccctg atgagggata atatttaaca
     4681 ttgctttaag caatgtggat tttccagcac catttggacc aataatacct gttaaagcac
     4741 cttcttgtat ttttaaagaa atctcgttca atgctaaaaa atcgttataa caaacattaa
     4801 gctttttcac ttctaacata actttctcct tctaaatgat aatgattatc acgatcatat
     4861 ggtatacaaa aattatattt tagtcaaccc taaattttat agtttagatt tttttctgtt
     4921 gctgaagatt aga
//
LOCUS       DASJUF010000157         4896 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.162, whole genome shotgun sequence.
ACCESSION   DASJUF010000157 DASJUF010000000
VERSION     DASJUF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.162"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(122..1732)
                     /locus_tag="U4B62_002706"
     CDS             complement(122..1732)
                     /locus_tag="U4B62_002706"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN2142806.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2925..4265)
                     /gene="glnA"
                     /locus_tag="U4B62_002707"
     CDS             complement(2925..4265)
                     /gene="glnA"
                     /locus_tag="U4B62_002707"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN2142807.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4293..4685)
                     /locus_tag="U4B62_002708"
     CDS             complement(4293..4685)
                     /locus_tag="U4B62_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2142808.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1441 a         1053 c          799 g         1603 t
ORIGIN      
        1 aagtcttttg ctttctatct tttttcctac aaataataac aaatcagaaa aagagaaaag
       61 tgacaagaaa attgcttatc cccttctctt gctacttttc tctttctatc aagttcaaaa
      121 attattttgt ttcgtgatct gctaatgatg ctccgacaaa tgctttgatt aatttttgcg
      181 ggcgatttgg acgagaaatc aattctgggt ggaattggca tcccacaaag aattttttct
      241 ctggcagctc tacgatttct actaaacggt tatctggtga gacacctgaa aagactagcc
      301 cttgttcttc aaacaattga cgatatttgt tattgaactc atagcggtga cggtgacgtt
      361 cttgcacaac atcggctcca tcatatgctt cagccgtctt cgttcctttt ttgattttac
      421 atgggtataa tccaaggcgt aaagttccac ccaaattctc gatgttttcc tgatcagcca
      481 ttaaatcaat aatattgttt gctgtatctg gattcgtttc agctgatgac gcatcttcta
      541 atccaacgac gttacgtgca aattcgacac aagccatctg cataccaaga caaataccta
      601 aaaacggaac gtcattttcg cgagcataac gaatcgcttc gattttccct tctactccgc
      661 gatcaccaaa tccaccagga accaagattc catctgcgtc tttcaaacgt tcttctacat
      721 tttctctcgt taattcttgt gctttgatcc aatcgatctc gatgtctgaa ttataggcga
      781 aaccagaatg tttcaatgct tcaactactg agatatatgc atctggtaac tcgacatatt
      841 taccgacaag cgcaatccgt acttttttct tcaagttcaa gacacgttct tctaattctt
      901 tccattctgt catgtctgct tctggtgtat ctagttttaa atggtcgcaa acaatttgat
      961 ccattccttg tgcttgtaaa actaatggga tggagtaaag tgtatctaca tctggcgact
     1021 cgataactgc ttctggttcg acatcacaaa attgtgccaa tttgtttttc acgctttgag
     1081 aaacaggttg ctctgtacgt accaccaaaa tattcggttg gatacctaaa ccacgtagct
     1141 cttttacact gtgctgcgtt ggtttagttt tcatttctcc agcagctttc aagtaaggaa
     1201 tcaatgttgt atggatatac ataacattat ctgtaccgac atctgctttc atttgacgaa
     1261 gtgcttcaag gaatggcaaa gattctatat cccctactgt tccccctact tctgtaatga
     1321 tcacatctga atcagtcatt ttagctgctc gcatgatttt ttctttgatc tcatttgtaa
     1381 tatgaggaat aacttgaaca gttgctccca agtattcgcc cttacgttct ttacgcaaaa
     1441 cttcagaata gatttttcct gtcgtcacgt tggagtattt gttcaaattg atgtcaataa
     1501 aacgttcgta gtggcccaga tccaaatctg tttcagcacc atcatctgtt acgaatactt
     1561 ctccgtgctg atatgggctc atcgtccccg gatccacgtt gatgtatgga tcaaattttt
     1621 ggatagttac ttttaagccg cggtttttca ataagcgtcc taaagaagct gcaacaatcc
     1681 cttttccgat tgatgaaacg acaccgccgg tcacaaaaat atattttgtc ataagttcaa
     1741 aaactccttt ttaatttatg tgcaggaaaa gtctcagaca ataaacaaag ctccctattc
     1801 ttcaataaga atagggagcc gaaatttctg taaacttctg accctctaaa agggtgccca
     1861 aggtgtataa tacaagctga ctaagggaaa gtcaagtact caaatgaata ctatccgctt
     1921 acaatttttt ctttttcgat atcctttgag aaattatttc ttaagtcctt tatgagtgca
     1981 aaaaaaattt aacccacata ataagcttga tataaacata gctaacgtta aacctaagca
     2041 aatcagaaaa tcgttactga aaaattttaa gtgaaataat agataaaaaa ataaaacaaa
     2101 cattatgata gataatatcc cacctaacaa agaactactt tttcctactt gcagcaacag
     2161 tttatatagc ttatcgtaga aacaatcttt ttctttcacc cttatttacc tcctttacgt
     2221 aaaaaacgta gtccacagtt ctcggatttt tggcatatct tttctattaa ataatatcaa
     2281 aaacaaaaca attccactaa ttttttcaaa gatgaccttt ttttcaacaa aagtgccttg
     2341 tagggattca ttgaaggtaa cgaatcacat aagtaaaaaa taaggagatg cgacaaaagt
     2401 cgtgcctcct tatttctaaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
     2461 aatatccatc aaacatgaga agctgttttt gaaattattt cttgtgattc ggagttaaat
     2521 ggtaggctca caacctctgg ataaacggta ttcaaaagcc agtttctcgg tattaagcca
     2581 aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttaatac tcgaaactaa
     2641 acgtcttttt caaaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag
     2701 ttcagccaat tcaaaaacaa gaaaagctgt ttttgcttgg ctattcttat tacttgttgc
     2761 agggcagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
     2821 agctcaaaaa acggcaaaat atgaggaact atttttccgt tagtttctct aatactcagg
     2881 tcaaaacgct gatttcacag cctctaattc acggtattcc tgttttaata aagttctaga
     2941 tattgttctc tttcccactc ggaaacagtt tgtctgaatg ccgcccattc gagacgtttt
     3001 gcttcaacaa aatttgagaa gatatgttgt cctaatgcgt caatcattac attatcctta
     3061 cgcaattctt tgatagcatt atggatagtt gaaggtaagt cctggatatg tgcagcttct
     3121 ctttcttcct catccattac ataaatgtta cggtcaacag ctgctggcgg tgtgatctga
     3181 ttacggatac cgtctagtcc tgcttgcaac aatactgcca ttgccaagta tgggtttgca
     3241 gatggatcaa ctgaccgcaa ttccaaacgt gtggataaac cgcgagattc tggtacacgt
     3301 actaatggcg aacgattacg gccactccat gcgacataaa caggcgcttc ataacctgga
     3361 acgagacgtt tataagaatt gactgttgga ttgcaaacgg ctgtgtaggc acgagcatgt
     3421 ttcagcaaac cacctaagaa atgataagct gtttcactta gttccaattc accgtcttta
     3481 tcatagaaaa catttccttc atcattgaac aaagacatat tgcaatgcat accagaacca
     3541 ttgatcccaa ataacggttt cggcatgaaa gtcgcgtgaa gtccatgctt gcgtgcaatc
     3601 gttttgacaa cgagcttgaa ggtttggata ttatcacaag catctaccac atctgcatat
     3661 ttaaagtcaa tttcatgctg tcctggcgct acttcatgat gtgaagcttc cacttcaaag
     3721 cctagacttt ctaattccaa cacgatgtca cggcggcaat tttcacctaa atctgttggt
     3781 gcaaaatcaa aataaccacc tttatcattc aaggtcgttg tgatcccacc gtcttcgtct
     3841 aatttaaata agaagaattc tggttcaggt cctaaattga aagaggtaaa accaaggtcg
     3901 cgcatatctt tcaatgctcg tttcaaattc ccgcgaggat cgccggcaaa tggtgtccca
     3961 tccggattat agatatcaca aattaaacga gcaacttttc catgagcact ttcccacggg
     4021 aagatcatcc aagtggagag atctggatac aaatacatat cgctttcttc aattcgaaca
     4081 aatccttcaa tcgaagaacc gtcaaacatc attttatttt ctaatacttt gtcgatctgg
     4141 ctcactggaa cttctacatt tttgatcgta cctaagatgt ctgtgaacat caatcgtaaa
     4201 aatcgtacgt tttcttcctc aatgattcgt ttgatttctt cacctgttat ctgtttcttt
     4261 accatggtat ccctcttcct tttttaattt attcagtcaa agaattgtct ttgcaatcct
     4321 tgcgactgat aatgacctgt tttgcttaac ccaccttgcg ccaaaatctc atcacgaaaa
     4381 atttgtcgaa cgtcatgatc tgtcaacggc ttcttgtttt ttgcttcttg ttttttcgcc
     4441 tgtgccattt cgtatacacg tttgatccca gccatattca atccctctga aagatagtct
     4501 ttaatctcaa gcaacatatc aatatcattc agcgaataca tacgtcggtt tccttcgctt
     4561 cgttccggat gaactaaatc ttgttcttca taatagcgaa tttgccgagc agacaaatca
     4621 gtcagcttca taactgtacc aattggaaat acggacatag atcggcgcaa ttccttttct
     4681 cccatcagac gtcccctttc gttttataag ttggctaaag tgtaccaatt aaaaaaaata
     4741 ttgtcaactg catatgttag gttttctaac atgagatctc tattttttat aaaataacaa
     4801 aaaaataaga acacaaaaaa ggtatctagt tgattttcgt accagatacc ttttattttt
     4861 tattatactg tactatcctt tattagtgtt tttatt
//
LOCUS       DASJUF010000158         4757 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.164, whole genome shotgun sequence.
ACCESSION   DASJUF010000158 DASJUF010000000
VERSION     DASJUF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.164"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="U4B62_002709"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="U4B62_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN2142809.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="U4B62_002710"
     CDS             724..1878
                     /locus_tag="U4B62_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2142810.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="U4B62_002711"
     CDS             2093..3061
                     /locus_tag="U4B62_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN2142811.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="U4B62_002712"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="U4B62_002712"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN2142812.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="U4B62_002713"
     CDS             4091..4699
                     /locus_tag="U4B62_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN2142813.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DASJUF010000159         4740 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJUF010000159 DASJUF010000000
VERSION     DASJUF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.165"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..535
                     /locus_tag="U4B62_002714"
     CDS             <1..535
                     /locus_tag="U4B62_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN2142814.1"
                     /translation="DNGQSYADNFTIQGLLEYASRDSGYDGVGDLNRITGISRVVDEI
                     FNNNFRLVLMGVGIGNAEFTNFFVSEFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLF
                     FIIKTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGPLIYMILAMPEVV
                     KRKEHYYSEKNTKGEIL"
     gene            532..1251
                     /locus_tag="U4B62_002715"
     CDS             532..1251
                     /locus_tag="U4B62_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2142815.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1266..2351
                     /locus_tag="U4B62_002716"
     CDS             1266..2351
                     /locus_tag="U4B62_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2142816.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2487..4019
                     /locus_tag="U4B62_002717"
     CDS             2487..4019
                     /locus_tag="U4B62_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2142817.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4197..4418
                     /locus_tag="U4B62_002718"
     CDS             4197..4418
                     /locus_tag="U4B62_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN2142818.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1659 a          504 c          925 g         1652 t
ORIGIN      
        1 cgataatgga caaagctatg ctgataattt cacaattcaa ggtcttttag aatatgcgtc
       61 aagagattct ggatatgatg gtgttggtga tttaaatcga ataactggta tctctagagt
      121 agtagatgaa atatttaata ataattttag attagtccta atgggagtag gcattggcaa
      181 tgcagagttt accaattttt ttgtcagtga gttttataaa tcgtttggta atcttcatta
      241 tcagtatttt catcatattt ggttatttat agaagttgga atattaggag tttcgatatt
      301 cctatttttt ataataaaga catttttcaa tgcaaaaaga tcttttatga aggataaaga
      361 tgcaagtctt gttaaagtta ttgcaattat gtcggtattc ttttttgtgt ataacacaac
      421 acttaggggt gaaacaacgg ggccactgat ttatatgatt ttagccatgc ctgaagttgt
      481 gaaaaggaaa gagcattatt atagtgaaaa aaatacaaaa ggagagatat tatgattcct
      541 aagaagatac attactgttg gttcggggga aatccacttc cgaaaaaaga tagaaagtgc
      601 atagaaagct ggaaaaagta ttgccctgat tatgagatta ttgaatggaa tgaatcaaat
      661 tatgatttaa ataagaaccg ttatatggcc gatgcatata aagaacgtaa atggggtttt
      721 gttccagatt atgcaagatt tgatattatt tataatcatg gtggatttta tttagataca
      781 gatgttgagc taattaagtc gttagatata cttcgtgaaa atcatgccta tatgggattt
      841 gaaggggaaa tctgggttaa tggcggtatt ggttttggtg cagaagaagg acattctgtt
      901 attaaaggtt taagggatat ttacgattca ttaagttttt atcaaaatga tggtacattg
      961 aacttgaaac cgagtccgta ttatattaca gattatcttg aatctattgg attggttcgc
     1021 gataacagcg aacaaataat aaatggcctg catatctatc ctacggatta ttttgctgcc
     1081 aaggattatg atactggaaa aatttataaa actagtaata caataagcat tcatcaatat
     1141 aatgcatctt ggaaaacacc taaacaaaaa tttgtttata gattaaaatc aacaattgga
     1201 caaaaaaaat ttaacaggtt acttgcactg aaaaaatttt tacacttata aaggagaaaa
     1261 gtactttgaa gaaaatttgt ttcattagtg gtgtaatttc tcgttctggg ggaactgaaa
     1321 gagtagggag tattattgcc aatagcttgt ctgatttagg ttatgatgtc actattttaa
     1381 gcttttggaa tagtggagag ccttattttg ggttagataa gacgattaag gttgattata
     1441 tgttaaatcc taaaacggag ggaaaattat atagaactta tttatatccg gtttggaaat
     1501 tgcacaagta tatatgcaaa aatgaatttg atatagtaat tgatattgat actttgttgt
     1561 ctttgtatac tacaagggca attaaaggaa caaagtgcaa attgatttct tgggaacatt
     1621 ttaattactg gacaatggaa atgctgaaag aaaagaagag attcaaggct aaaaaattga
     1681 taaaaaaata tgcaaagagg cttgtggttc tcacagaaga agatcgtcaa aaacatattg
     1741 atgaatatag tttatcagaa gattttgtta taactatgcc aaatccgtgt atatctaata
     1801 tcgagtacga atatcattat gataaaaaga gatttcttgc agtagggcga ttagtggaag
     1861 agaaaaattt tgctgacatg ataagtgctt ggaaaattgt agaaaaaaaa gtagatgatt
     1921 gggatcttat cattgtagga aaaggtgaat tagaaaaaga tcttcgccag cagataaaag
     1981 atttaaatct gcaaagagta actatagcag ggcattcaga ccatgtggag gaatattata
     2041 aatctgcatc ttgttatgtg cttagttctg tttatgaagg atttcctatg gttatattag
     2101 aagcacagtc ttatggatta ccagttattt cttatgattg taaaactggt ccgagagatt
     2161 tggtacatca taattttaat gggatgttgg ttgaagataa gaatatagac caattagcta
     2221 agagcatgat aatgtttact aagaatactg atttagcaat gagaatgtca ctgaacgcat
     2281 ataataatgt acaaaaattt aatttgaaag aaattacaaa acaatgggtt gctttgattg
     2341 aaaatgtata gttttttgtg gttgtccaaa catgaaaatt agcgtaatta atcaaagagc
     2401 tagaagaaac ctattacatt tcaagatttt agaaattata tagtattagg taacaataaa
     2461 aaatagaaag tttggagcca tatgatatga taattaatcg taaacaaaat gctaaaagga
     2521 atataattac tggaacaatt tttaagttat atcagattat cattcccttt tttatgcgta
     2581 cacttatgat ttatcagatg ggaatggagt atgctggatt gaacaacctg ttcacatcta
     2641 tatttcaaat gttaaattta gcggaattgg gaataggtgc tgcactaact gtaagtatgt
     2701 ataaacctat agcagaagac aatagaaaag agatttgtgc tttactgaac gcatataaaa
     2761 agtacttttc aattattgga tggatagtgt ttacattagg attgttatgc attccttttt
     2821 tgagattttt agttaaaggt tcgattcctg gaaatttaaa tatgacagtt ttgtatttaa
     2881 tgtatttgat aaatactgtt ttgtcgtatt ggttattttc atatagaaag agtctaatat
     2941 atgcacatca aagaaatgat gtaattaacg tgtctatgct aattactttt acattccaat
     3001 ttgtaatgca ggcatatgtt ctgattttct taaaaaatta ctatttgtat ttattggctt
     3061 ctattttggg acaaatagtt cttaacttaa tgtgtgctgt tgtttctcaa aagatgtatc
     3121 ctgattatac tcctgttggt gagttagaga aagaaactat tgacgatatt ataaaaaaag
     3181 taaaaggatt agttacaaac aaggtaggcg gtacaatatt acgatcttca gattcaatta
     3241 ttatttcatc tttcttaggt ctttcagtac ttgcgattta tcagaattat tactttatta
     3301 tttctgccat aatgagtata tttgcaataa tttatgaatc atgtatagca ggtataggta
     3361 acagtcttat tgtagaaagt caagaaaaaa attatataga tttttgtgat attacaacaa
     3421 ttataggttg gtttctggca attagttgtg catgctttat tacattattt cagccgttca
     3481 tgattatctg ggtgggaagt gataatctta tgagtatttc atttgttata tttatgatag
     3541 ttcatttttt cttatatgaa attgatcagt taattgggtt atacaaagat gctgctggaa
     3601 tatggtacgc agacaggttt agaccattaa ttacagcaat gataaacatt gtattaaata
     3661 tcatcctcgt taattggttc ggattatatg gagttttggc ggcaaccatt gtttcagtat
     3721 tgttggttga tttaccctgg ttattatata atgttttcca aaatatattt aaatttgaat
     3781 cggcaggtaa atatgtttgg aagctcataa gtttgtgtgt aatagctttt gttgcttctt
     3841 atttaagtta ttttgcttgt tcgtatgtta atatcggaaa tttcaaagtg ataagtttag
     3901 tgataaattt agttatatcg gttgtcatat cgattttagt tattcttctg tttaatatcc
     3961 gtaataagaa gtttaagtat attttgaaag ttcttacgtc ggtattggta aaaagatgag
     4021 aagagacatc agaatgatat ataaagtaac tacaattttt aggctgccga ataggcgaac
     4081 gatgtatagt ttttgttcca acgaagactc ttgttgaaat tggagatgat atgaaggagt
     4141 tacaattttg actcatgttt atgattgatc ggttttaaaa gtagtatgga gaagctttgg
     4201 gatcatcgga gaggatcaaa ataggtaata atgtttttat aggaatgaag tctagaatac
     4261 taaagggtgt acatatagga aataatgtag ttataggtgc aaatagttta gtaaacaagg
     4321 acgtatcaga cgattgtgtt gttgcaggaa atctagcaaa agtaattatg tccctagatg
     4381 aatattataa aaagataaac aatatgaaga atccgtaaaa cttgtgaaac tataccgaga
     4441 ataatatggg aaaaatattg atgaaaaagc attatatgag tttttagggc tgtttacaga
     4501 tggagatgac aatttgtcga agagttggga gcaatgcaaa atttagtagg ggattataaa
     4561 cagactgaca aggtatttaa agaacatgca caagtatttc ataatattga agaatttttg
     4621 aatactgtga gataggagtt gctacattga gaaaagtatg tatttttcca atgtaaaaga
     4681 ttacgagttt gaaaacgaca ccataatatt attttttaag aagatattat agagcttagg
//
LOCUS       DASJUF010000160         4695 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJUF010000160 DASJUF010000000
VERSION     DASJUF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.166"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..406
                     /locus_tag="U4B62_002719"
     CDS             <1..406
                     /locus_tag="U4B62_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142819.1"
                     /translation="WKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSES
                     SVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLET
                     EKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            627..2390
                     /locus_tag="U4B62_002720"
     CDS             627..2390
                     /locus_tag="U4B62_002720"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN2142820.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2474..2932
                     /locus_tag="U4B62_002721"
     CDS             2474..2932
                     /locus_tag="U4B62_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN2142821.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3041..3724
                     /locus_tag="U4B62_002722"
     CDS             3041..3724
                     /locus_tag="U4B62_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2142822.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3871..4485
                     /locus_tag="U4B62_002723"
     CDS             3871..4485
                     /locus_tag="U4B62_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN2142823.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4501..>4695
                     /locus_tag="U4B62_002724"
     CDS             4501..>4695
                     /locus_tag="U4B62_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142824.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1659 a          757 c          974 g         1305 t
ORIGIN      
        1 gtggaaaaag aaaaatcaaa tccaactaag atggaatgaa aatttcattc gaaaattcgt
       61 caaaagtctt tctttgatca acagtgaaca agcggggaga caggaaatag aaatttttgt
      121 tgtttcagaa agttcagtca aacaattttt ttatcgaacc caatttcatt tgtatgcgga
      181 agaaggcatc gctgaaataa atcctatttt atttcgttca ttagaagaat tgcctgatga
      241 atgtctatgt gcgaaaaaaa gagtggtttt gtgcgatgct gctagctatc aaaatggatt
      301 agaaactgaa aagacaaaga tctatccaat ctcattaaaa ggtgcaaaca tccatttgta
      361 tcatattgct caggaacttc acctattaaa aaaacatatt tcttgatgag accttaatta
      421 ggagaccttc tacaaagctt tttaattgtc tgtagaaggt ttttatttga gtcctgattc
      481 gtgcgaagat ctacttatat aacaagcttt ctctgaaatt tttttaaaat gctattgact
      541 tagagtcaac tctaagtagt agaatagata aagtgtaaca aatctatttt cattaaaaga
      601 agatagtacg gaggagagaa tgtattatga gtacagtatt acatgtattg gatgcaatca
      661 atagccccgc agatgtcaaa aaattatctg ttgaggaaat gaaacagttg gcagaagaga
      721 tgcgccaaaa aatcttgcaa aaggacagcg atgtaggtgg gcacgttggt ccgaatctag
      781 gtgtagtaga attaacgatt gcgtttcatt atgtatttga ttcaccaaag gataaaatcg
      841 tttgggatgt ttcccatcag tcttatggtc ataagctttt aactggtcga aaaaatggtt
      901 ttgaaaaagg acattaccgt gaagtgagcg gatattccaa ccaacatgaa agtaaacatg
      961 actattttac agtggggcac acatctacat ctatcagctt agcaacaggt ttagctaaag
     1021 cgagagatct gaagaaagaa tcaggaaatg tggtagcatt tatcggtgac ggctccctaa
     1081 gtggtggctt agcttttgaa ggattgaaca atggtggtgt tttacaatcc aatctaatcg
     1141 tattagtaaa cgataatgag atgtctattg atgagaatca tggcagttta tataaaaact
     1201 tagctgagct tcgtcaaacc aacggtaaaa gcagcaataa cctatttaaa gcaatgggat
     1261 ttgattatcg ttacgtggat aatggaaacg atctggaaac ggtgatccgt ttatttgaag
     1321 aagtaaaaga tattgatcat ccgattgtac ttcacgtcca tacggaaaaa ggtcgcggtt
     1381 atcagccggc aatcgataat aaaatgaaat atcattggca tgtaccattt gatctgtcca
     1441 ccggggaggc aaagcaagta acttctggag aaacatttaa tatgatcatc atggattatt
     1501 tggataagaa aatgcaaaca agtacagctc ctattgccgc agtcaatgca gcgataccag
     1561 gcgcctttgg actgaaaaca ttccaagaaa aacatccaga tcattatttt gacgtgggga
     1621 tcgctgaaca gcactctatc actttcgctt ctgctttggc ttctcaggga gtacgtccag
     1681 tcgtcttcca atcaagtact ttcttgcaac gggcatatga tcaattatca catgatttag
     1741 cgatcaacga gaatccagtg attattttag tcggtggcgg aacgatctca gctagtgacc
     1801 gaacacacca aggaacattt gacattcctt acttatcaag tatcccgaac atcaaatatc
     1861 ttgctccagc aacaaaagag gatatgatct ctatgctaga ttgggcgttc gaacaaacag
     1921 cgcagcctgt agcaatccga attcctacac atggtgtaga acatggtaca gctatcgcaa
     1981 atgattatag tattccagcc tatcaaacag taaataatgg aggaaaagta gcaattttag
     2041 ctttaggcgg ttttcttgaa ttaggaaaag aaacagtaaa agcttttaga aaaaaaggaa
     2101 tcgaagcaac gttgatcaat ccaggaacga tcacagaaaa tgacgtagaa acattagata
     2161 aattaaaaga aacacacgaa ttagtcatca cattagaaga tggtagtttg tcaggaggat
     2221 ttggcgaaaa tatcagtcgt tattatggtg attcttcaat gaacgtatta aattttggtg
     2281 cagagaaaga attcacggat agcgttcctg tagggcagct ttatgaacgt tatcatttaa
     2341 caccaaaaca aatcgttgaa gatagtctgc gtgtcttgaa tattcaataa ttcattatgc
     2401 tcaccatcca gataattgtt tatactagta tctggatggt tttttcatta taaaaagcaa
     2461 aggggtggat gttatgtatc agcaagtaga agaatatgtc aaaaagttcc aagaaaaata
     2521 tcctagttat caatcagaaa aattaggttt cttttcattt ggggatacag aaaaaatggc
     2581 agacgagttg agcgaactag ttgtaaaaaa gataaagact ggtacatcaa gtggttacga
     2641 tctgtataaa caagagaatg agcctttacc aaaagttggt caattagacg tggtcctgaa
     2701 tggaaaaaat gaaccagttt gtatcatcaa aaatataaat gtgaccatct tgccttttag
     2761 tgaagtcacg aaagagcatg cttggaaaga aggagaaggg gatcggacat tagcttattg
     2821 gcggaaagtc catgctgagt tttttaaaca tgcttataaa gaagcgttga acatagaatt
     2881 tacagaagag aagctggtcg tatacgaaga atttgaagta attttctttt agtaagaaag
     2941 gtctgctgaa agaaatagct cattattgtc tcctgaatag aaaagggtta catttttata
     3001 tgaagctgta tttttatata acattactgg aggagatatt gtggatacac tggaacaaat
     3061 caaaggcgga gtcgtcgttt cttgtcaagc tttggaaaac gagccgctac acagttcttt
     3121 tattatgggg agaatggctt tggctgcaaa agaaggcgga gcagttggta ttcgagcaaa
     3181 tacgacagaa gatatcaatg aaatcaagcg aatcgttgat ttaccggtaa tagggattat
     3241 caaaagagat tacgatgggt cagaagtgtt tattactgcg acagaaaaag aagtggaaga
     3301 acttttggag acgcaagcag aaatcattgc actggatgca acttctcgta aacgtccgac
     3361 agaaaaaaca actgctgaac ttgtccaaat gattcacgaa cacggtcggt tagctatggc
     3421 agatatttcg acttttgagg aagcgcttca agcgcaagaa gatggttttg atcttttgtc
     3481 tacgacttta gcaggctata cagaatattc acgaaagacg cagacacctg attttgattt
     3541 attgaatcaa atcatagaac aagtcgatat cccagtaatt atggaaggac atactgactc
     3601 tcctgaacaa gtagagaaag catatgaatt aggtgctttt tcagtcgtag ttggatctat
     3661 tatcacccgt ccgcaattga tcactaaaag atatgtcgaa gccgcaagta aagcaaataa
     3721 ctaaaaaaca cgcacggcac agattcttga caaaaaagaa aaattgatag atagtttaaa
     3781 gcagattcta agaagatgaa aaaagcatta cttttcattt tgtcttcaag atctgctttt
     3841 taacgaattt tatagaaaga aggcgaaacc atgtttgaac ggagtccttc agaattgaca
     3901 gaacgtgaaa attacaagtt gctgattggt tcagtcatcc ctagaccagt agcagtagta
     3961 acgactaaat cagcaaaaga tatagtgaat atcgcaccct tcagttactt caatatcgtc
     4021 agttcgaatc gacccatctt atccttggcg atccagagaa aacaagatgc agtaaaagac
     4081 acagcaaaga atattcttga gacaagagaa gctgttatcc atatccttga tgaggacaac
     4141 gtagaagaag taaataaaac agcagccaat ctgcctaatg aggaaagcga actgtacctg
     4201 actgacttga ctttaactga ttcagtaatc atttcagtac cagcattgac agaagcaaaa
     4261 gttcgttttg aaacatcctt atatgaacat gtcgagatac ggaataaaaa taaagtaaca
     4321 gctgatttat ttttgctaga agtacagaaa tatcatttaa atgaaacagt ctatgatgat
     4381 aagacaggaa ggatcgatcc aagaaaattg aatgctgtaa gccgtttggc aggaaatgat
     4441 tatgcagcaa taggagaaat tttcacgatt gcacgtcccg aataaaaagg agcactagtt
     4501 atgttaaaaa taagctataa accatcgaca gattcaaaag aaatgaaaaa agaatatgaa
     4561 acagtgaatg attttctgca agggcagtac ttagaagttc cgccattgca agaccatttt
     4621 gtcgttacta ctgttacttt agatggtaag gaaatagaaa tgccagatca aacgattagc
     4681 ggattgttta attac
//
LOCUS       DASJUF010000161         4669 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJUF010000161 DASJUF010000000
VERSION     DASJUF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.167"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(64..426)
                     /locus_tag="U4B62_002725"
     CDS             complement(64..426)
                     /locus_tag="U4B62_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142825.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(500..880)
                     /locus_tag="U4B62_002726"
     CDS             complement(500..880)
                     /locus_tag="U4B62_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN2142826.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            944..2137
                     /locus_tag="U4B62_002727"
     CDS             944..2137
                     /locus_tag="U4B62_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2142827.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2214..2798)
                     /locus_tag="U4B62_002728"
     CDS             complement(2214..2798)
                     /locus_tag="U4B62_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2142828.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2999..3997
                     /locus_tag="U4B62_002729"
     CDS             2999..3997
                     /locus_tag="U4B62_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2142829.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4109..4618
                     /locus_tag="U4B62_002730"
     CDS             4109..4618
                     /locus_tag="U4B62_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN2142830.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1478 a          883 c          828 g         1480 t
ORIGIN      
        1 accaaacaat aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg cttaatctgt
       61 attttagaag aaaggcactc cctctacttc gtaagttcca agaacagagc gcatttgacc
      121 tggttcttcc caaatatatc cttcgcctaa ttcttctctt tgattctttt gttcagtttc
      181 aaagacaaac aacggaacat tttcctcttt aatatgtgta tttgtcaatt ccatcaaact
      241 aatcttgcta acatcgatat gaactatttc ttttaagaaa ttaagtatat tcgctaaacc
      301 agtctttcct tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa ttgaatggac
      361 caaaaatttt ttagtaccat cctccaaatg caacataatt gtaccagcaa ctcttttgct
      421 tgccaaaatt tccacctcga tcaacttgtt ctatggctta cataaaaatt ctagcacatt
      481 ttcagaaaaa atacactttt tatttcttat tttctttccg ttctagttca gataatgctt
      541 cttctatcca aataggtaac tgctccgcaa tagtggtaaa tccgatctta cgatttttat
      601 tcgtgaaata cctttttctg cgatatggaa cagctggatt tgttttttct aacgttctta
      661 aagataaact gatttttttg gaatattcat caatgtcgat gatctgaact gttactggct
      721 gtccgacttt taacaatgaa tggatatttt tcgtataacc ggattgtact tctgaaacat
      781 gaataagtcc ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc tgtatccctg
      841 tcacttttcc ttcaattatt tgtccaattt tataagtcat ctacttccct ctcttcgcat
      901 ttatcattat actgtaaata gctcaaaaaa agaagcgacg aaaatgactg gaggtcttaa
      961 gatgatcgaa tttgataaac agatttcaag aaaagataca gaaagtgtaa aatgggacgc
     1021 aattgctgag acatatcaaa tggatgactt acttcctttg tgggtagcag atatggactt
     1081 cttgtctccc ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg gaatctttgg
     1141 ttatgccact ctgtctgata aactatatca ggctatcatt cattgggaac gccaacacca
     1201 tgctgtggag ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa ccagtttagc
     1261 tgcagcagta caaactttta caaaaccagg cgattctatt ttgattcatg acccagttta
     1321 tcctccattt tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa gctctttgct
     1381 agaaaaaaat aatcatttca tcatggactt gtctgacatg gagaaaaaaa tcgaagaaaa
     1441 caatgtaaaa gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg tttggagcaa
     1501 agaagaattg aaacagttaa gcgaattgtg tttaaaacat caagtgattc tgtttagtga
     1561 cgagatccat caagatttaa ctttattcga tcatacattt acttccatgc taacgattga
     1621 tcctaattta gatgatctac tagttgcttt tacttccgca accaagactt tcaatctagc
     1681 tgcaatcaaa aattcaatgg tatttatcaa aaatccagag atgaaagctg attttgaaaa
     1741 acatttagtg atgaaccaac agcacgaaat caacactttt ggactgattg gaacccaagc
     1801 ggcttatgag acgggtggag aatggttaag ccaactgatt ccttatctag aaaaaaatgt
     1861 tcaaacagtt aatcagtttt ttgaacagta tcttccgaaa gtgagaataa tgaaccctga
     1921 aggaacttac ttaatgtggt tagattttac cgcctatatt tcagacgacc aagaattgga
     1981 gaacactctt gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt ttggtccttc
     2041 cggtcatggg catatgcgct tgaatattgc ttgtccagaa gaaactttac tggaaggact
     2101 taatcgaatc aagtatgctt tagagacaat cgattaaatc atttattcaa aataaaagga
     2161 cgagacctta ctaggtttcg tcctttttca taattactcc gtaccatacc aaactattct
     2221 gaaatttcaa ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg accacgttgt
     2281 acatttgcga tttcatctac tgtgtccatc ccttctagta catgtccaaa gactgtatgt
     2341 cggaaatcta gccaaggagt tccgccacct ttatacgctt caatgatttc tgatgggaat
     2401 ccagcacctt ctagttgtcc aagcatatta gctggcacat ttgtgttatt tacaataaag
     2461 aattggcttc cgtttgtgtt tggacctgaa tttgccatag ataaagctcc gcgaaggttg
     2521 aacagttcac gactgaattc atcttcaaaa ctttcgccat aaatactttc gccacccatt
     2581 cctgttccag ttggatcgcc gccttggatc atgaaatcag gaataacgcg gtgaaaaatc
     2641 acgccatcat aataaccttt tttggaaagt tcaatgaaat tttgaaccgt cttcggtgct
     2701 agttcaggga ataattgaac agtaatgtca ccacggtttg ttttgataac agcttttggt
     2761 cctttttcac tttttaaatc tagttgtggg aatgccatat tgtttcctcc taatatttgc
     2821 atacttcata ataataataa cataatacct aacctctcgt aaaaatacta gatttgtttt
     2881 cttttatttt tcatcactag atacattcta taacttaccg tttcgatttc gaattcaaac
     2941 atttttgaga agcattctca attaaattct gctatagtgt gaaaagggag gaatttttat
     3001 ggaaatctat gacattacaa tcatcggtgc aggtcctgta gggatgtttg ccgcttttta
     3061 cgcaggaatg cgccaagcta aaacaaaaat cattgacagt ctacctcaac ttggtggtca
     3121 attagcaact ttgtatcccg aaaaatatat ttacgatatt cctgggtatc cagcaataaa
     3181 agcaagtgaa ttaatcgatc aattagaaaa acaattaact acttttaacc atacattcca
     3241 tttaaaagaa gaagttctct cgcttactcg tgaagacgaa gttatcgaga ttactacaaa
     3301 taaaggcatc cattattcca aagcagtcat tttagcttta ggaaatggtt cattccagcc
     3361 aagaaaattg aatttagata atgctgaatc atttgaaaac cacggattgg attatttcgt
     3421 gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc
     3481 tatcgattgg acgttgatgt tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg
     3541 cccggaattt cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat ctgtcaactt
     3601 gcttactccc tatctcatcg atggattatc aggcaataat ggggaactga cagatatccg
     3661 gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg attgattctc tgatcgtcaa
     3721 ttatggattc tcttctaatt tagaacatct ttcttcttgg ggacttgata gtaccagaaa
     3781 tgccattact gtaaaatcag atatgtctac ttctattcca ggcgtttatg cagctggtga
     3841 catctgtacc tatgaaggaa aagtgaaact gattgctact gggctcggtg aagcacctac
     3901 tgcagtgaac aacgctttac actatattaa tccaaaggaa cgaactcagc ctggtcacag
     3961 cacaagctta tacgataaaa atcttcgtcc tagttgattt tttatatctc tttgcatttc
     4021 aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca tgaaatagag
     4081 aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa actgagactt tagaagaaaa
     4141 ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa gatatcgcac aacttgtcat
     4201 gtttttacaa aaagactata tttctgattt gactttggcg gattgtactg aaagtgtcaa
     4261 cagcgtgcta acaaagcgtg aagtacataa cgccattatc actggaatcc aattagatat
     4321 attagcagaa aagaaaaaat tgatgagtcc gttgcaacat attatcgaag atgacgaagg
     4381 cctttacggt atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg
     4441 ctttacgaat tatggttata tcgacaaggt gaaacctggt attttaaaag aactaaacag
     4501 ccacgatggc gttcatgtcc ataccttctt agatgatatc gtcggagcga tcgccgctgc
     4561 tgctgccagt cgattagcac acaaagaacc agaaaaaaga agtcgcctta ctcaataatt
     4621 caacaaaaaa agaaagaaaa cgtctattta gacgagttct ttctttttt
//
LOCUS       DASJUF010000162         4484 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJUF010000162 DASJUF010000000
VERSION     DASJUF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.168"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="U4B62_002731"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="U4B62_002731"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN2142831.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="U4B62_002732"
     CDS             complement(1034..1954)
                     /locus_tag="U4B62_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2142832.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /gene="xth"
                     /locus_tag="U4B62_002733"
     CDS             2067..2819
                     /gene="xth"
                     /locus_tag="U4B62_002733"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN2142833.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3683..4216
                     /locus_tag="U4B62_002734"
     CDS             3683..4216
                     /locus_tag="U4B62_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN2142834.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1488 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3361 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3421 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3481 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3541 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3601 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     3661 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3721 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     3781 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3841 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3901 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3961 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4021 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4081 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4141 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4201 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4261 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4321 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4381 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4441 ttggacttat taccgcagat gcaagctttt ggacaccgtt tacc
//
LOCUS       DASJUF010000163         4458 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJUF010000163 DASJUF010000000
VERSION     DASJUF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.169"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(144..1910)
                     /locus_tag="U4B62_002735"
     CDS             complement(144..1910)
                     /locus_tag="U4B62_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142835.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1870..3624)
                     /locus_tag="U4B62_002736"
     CDS             complement(1870..3624)
                     /locus_tag="U4B62_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142836.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3734..4303)
                     /locus_tag="U4B62_002737"
     CDS             complement(3734..4303)
                     /locus_tag="U4B62_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN2142837.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1506 a          834 c          616 g         1502 t
ORIGIN      
        1 agaatagaca ttaagtgaac taattcaatc atactgattc tttcgtgtat tcaaaattag
       61 atgctgttta ataaaattat tctttttgaa aaagaattat aactatatcc caaaaattgt
      121 aggttaattt aaagcctatt tcatcatcgt gattgagctt ctttttcata gacgaatttc
      181 tcagatagtt tagttttctt tatgagattt ttgtatattt ctgaccttcg atacaactca
      241 ttatgagtac cttgatcaac tagttttcct tgatccatca ctacaatttt atttaccttt
      301 tcaatagact tcaagcgatg cgatataatt agtactgtct tatcttgtat taaacgattt
      361 aaactttcct gaatacgttt ttcattttcc gcatctaaac tggcagtaat ttcatctaaa
      421 attaaaattg gcgcattttt caaaaaagcc cgtgctatgg ataggcgttg tcgctctcct
      481 ccagaaagtg aacttccgtt ttctccgacc aatgtatgat aaccgtcagg tagtttttca
      541 ataaagtctt cgcagttagc taaacgagct gcttttttca cttcctcatc tgacgcttga
      601 ctatttccaa tacgtatatt ttccaaaata cttgtattaa acaaagttac ctcttgaaaa
      661 acaatagcga ttttgctaaa caaagaagct ggagatacac gtttaatatc atacccatca
      721 attaaaatac agccttcatc ataatcatac agtctagaca ctaatttcaa gatactagtt
      781 ttcccggaac cactagcacc tactaacgca gtcacttctc cttgtttagc cgtaaaagag
      841 atatgatcta aaattggggt attgttatca tatgagaaag agacatctcg caactcaatg
      901 tcaaaagatt tcagaggact atctgatccc tcttggatac tggtttcctt catagctcta
      961 attcgctgta ttttaggagc taaataaaat atttcaagca ctgcttcttt catagaatcg
     1021 aaggaatcct ttatttttat tgcggctagt aagtaaccaa cgacgtagag tatagtcact
     1081 tcacccgtta acagtaagtg aacacctact agaatgacta ctgctaaact aacatatgag
     1141 aaaatagagg ataaggccat gactgaaaaa gtacttaatt ctacctttaa atgaatcctt
     1201 tcactctctt ccattttttt gaaaagtctg tcttgaactt ttttagatag attaaagcta
     1261 ttaatctctt gctgcaattc tatagtttct tgaaattctt ccgagttttc tctcaaagta
     1321 tcgtaatatt tagtattggc tttcgtttgg gatttctttg ataacaagat taagacaaaa
     1381 ctaaataacg ttggcaaaat aacagctaat cccattttga cattaccaac caaaagcatc
     1441 actgaaataa aagggaaaaa cagagccata ccgccggctt taggtattgc atgactcatc
     1501 gcatgctcaa taccttcaac gtcagacatg atagtttgag ataaatctga taaattatgt
     1561 tttgaaaaat aggataatgg taaatttgac aaatcgtctg caatttgcac tcttaaatgc
     1621 gcactttctt tataagtact attatataag ttttcatact ccctatctaa caaccaatac
     1681 atgaaaagca aagtaacaaa cgatacggca aaataaagcc aacggggctt tgtattttct
     1741 aaaactaatt cgtctatcag catcattata atgaatgctg gtaccatatt aatgcagtat
     1801 acaaaaaaac tagaaataga agctttagtt aaagcttctg cgccctgatc acttaaagca
     1861 aatttgtttt tataaaaact tttcattgtc aagcctccaa tcatttgcat tctcataatt
     1921 agtgaccaat tgtttataca cttgatgttt agccattaat tcattatgat ttccacgttc
     1981 tactattttc ccttctgaca agacaagaat ttcatctaca gatttaatgc tggataatcg
     2041 atgagctatc ataataactg ttttttcttt catcagatgt tgaaaagcct tttgtagttc
     2101 atactcatta tctggatcaa tagatgcact ggcttcatcc ataataacaa tctttgcatc
     2161 ttttaaaatt gctctggcaa tagctatacg ttgtttttct cctcctgaca gataaactcc
     2221 tttactacca attaaggtat gctctctttc gggaaatttt tctaaaatcg attcgcaacc
     2281 ggctaatctt agcgcgtcaa atactttact tgctggcgct tgaggatttg ctatagaaac
     2341 gttatcgtaa atactctgat tgaatagttt cgtatcttga aagacaaaag caattgtgtt
     2401 aataattgtg tcttttgaat agtgatttaa aggcttacca ccaattttaa tctgtccctt
     2461 atccacattg tagaaacctg aaattagttt agctatcgta ctctttccgc ttcctgattt
     2521 tcctactaat gcataaattc ttttttgttc taaaactaag ttgaaatttt ctaaaacaca
     2581 ctcttctttg tatgaaaagc tgacattttc aaatgcaatg tcacaatttt taatctgtgt
     2641 ctcgttccca tattttagtt ggtcttgctt catttctaaa taaagggagt ctagtgtttc
     2701 gactgcatat cttgcttcaa acaagtgcat acttacatac attattctca tgaaagaaac
     2761 aaaaataatt ccacttaaaa aaaacaacat caacaagtca acagctaata tcttgggagt
     2821 ggtaagagaa gttataaaaa aggttatagg aactatcaaa atgcaaacta tgccaaaaaa
     2881 tagccattga aaaagaacaa aaggtgtttt cccactcaaa gaatacttat atgcaaactt
     2941 agcataatcg ttaattgctt tattcaaggc tttaaatgat gttactttag caccaaatat
     3001 ctttataact tgaattccgc ggatatattc taccgtttca gagcttaaca catctaacga
     3061 ttcttgatat ttttgaataa aatttgtatt ctctgtcatt ttctttaaca agaatacacc
     3121 aattactaac atggcgatta gaaccaatcc aattttaaag ttaatgaaaa agcctaaggc
     3181 aactgataaa acaggtgtta ctatggcttg agcactatct ggaatcaagt gagcaattgc
     3241 catatgtgtt ttagccgcgt tgtcatcaat tgtctttcga atatatcccg aagaatttaa
     3301 atcaaaaaaa cgaaaacttg cttctgttaa accgctaatt cccttttttc tcaaattagt
     3361 ctctaagcga aacccaagga tatgagaaaa taagccagaa aaaatataaa ataaagctcc
     3421 taaagttaac caagcaacag tttgaaccgc ataactctta gctaaatatt catctcctac
     3481 aataattaga gactgaagaa atttgtaaat actgtaatat ccatagactg ttaatacgac
     3541 agaaaaatca gaaaataaag tggctaacca tccgtagtat ttcattttag ggacataact
     3601 aaataatttt ttgtatactt tcattttttc ctcccccgca ttcaatttct tctcttattc
     3661 gtttacaact gttaatgctt tccgtaatag cgtaaattag agatgtacaa acagataaaa
     3721 ggtaaactac gttctatttt ttgtattttt ttaagacttt tcttccaatc aatacaccaa
     3781 tcatagataa acttgctaca actaacactg aaatgattcc ccatatacct accgcatagt
     3841 ggatcattgc atccattgtt gcagcagatt gtcctttatc tatccaatct tgacgaaaac
     3901 gatctgtaaa gaaccatata ggaatccact gtccggcagc actgcctaat tgcattagag
     3961 cccaagacgc aacttgagga attgctcgat catatccaaa tacagataga ataatatcag
     4021 ctaagatcgc catactaatc gaactgataa accaaggtaa gtacgccccg cccattaacg
     4081 taaaaaataa caataaaaca attgtgtaaa tagtaaaaat tccttttcta gggtagttat
     4141 agctaatata aacaaatatg attccagata ataatccaaa aatcccgggg gatatgctgt
     4201 gaccaaacgc cccaccaata ctactaacca ttattgaaaa aaagtaaatt actagggaaa
     4261 taactgtagc taaaccaata aaaatataat cttttgtttt catatatatt ctcctcaatt
     4321 ctatttcgtt atgtcattaa aaatctgttc tatcaaagtt gaatcttcta ctctaagttt
     4381 tatatctttt tttattccac cattttttaa ataaataatt ctattgcatg ttttataaag
     4441 aaattccgga tcatgagt
//
LOCUS       DASJUF010000164         4429 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJUF010000164 DASJUF010000000
VERSION     DASJUF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.170"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            62..733
                     /locus_tag="U4B62_002738"
     CDS             62..733
                     /locus_tag="U4B62_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2142838.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(1452..1622)
                     /locus_tag="U4B62_002739"
     CDS             complement(1452..1622)
                     /locus_tag="U4B62_002739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142839.1"
                     /translation="MVLIKSMNYSGGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKD
                     YSALGLFSIGYW"
     gene            1746..3065
                     /locus_tag="U4B62_002740"
     CDS             1746..3065
                     /locus_tag="U4B62_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEN2142840.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3254..3655
                     /locus_tag="U4B62_002741"
                     /pseudo
     CDS             3254..3655
                     /locus_tag="U4B62_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            complement(3960..4274)
                     /locus_tag="U4B62_002742"
                     /pseudo
     CDS             complement(3960..4274)
                     /locus_tag="U4B62_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         1487 a          788 c          685 g         1469 t
ORIGIN      
        1 tattatatgg catgaacaga attaaaatat cagttaaaat aaaagttaag gagcattgtg
       61 tatgacaatt aaaactagtc tccaaaatca agcttaccaa tctattcgcc gacaaattat
      121 ctatgctgac ttagaacctg gaaaaaaagt atcagaaaaa aaattagaag aaacactttc
      181 tattggtcgt acaccaatac gtgaagcact gattcaactc cgacaacaag gtcttgttga
      241 tacaattcct caaagcggaa attatatctc attaattgat ttagattcgg ccaaaaatgc
      301 tcgatttgtc agagaacatt tggaacgaca aattatgtta gagtgttgtg ccaaaatgaa
      361 taaccaaaaa aaacggattt tagaaactat aatttcagaa caagaaaaag ctgttagtca
      421 aaaaaatgct cgtagttttt ttcataatga taatttattc cataaagctt gttttgaaat
      481 tgcagagcga gaagaagtat ggcaatggtt agaaaataac aatacacact tagaacgatt
      541 tcgatggcta cgagtaaaaa cggaaggatt aaagtgggaa aaaattatgg aacaacatta
      601 tcaactattt gaggcattaa caaaacaaga tccagaagaa gctaactttc taacatcgtt
      661 gcatcttcat ttaatgctta atgagcaaaa tattgtttta gctactcatc caaaatattt
      721 caaaaatata taacttttaa ctctttttaa attaaatggt tgttctgatc agaaaaatat
      781 cgcttgtttt cttattatgt aatatatttc ataaactatt ctgcataaca actattgttt
      841 gtacttttta cattggataa ctaattatga ctgctgataa cttttgatat taaactagta
      901 tctccaatac aaataaaaaa tagatatctt gttcatcaaa attagttcgt ggatacttac
      961 cttcatttac atcattcagc ggtttcccat tttcttttat tctattattt cattttctaa
     1021 tagacttggc ttcatttcgc aatacttttt ttcaataggc atcaaattgg tgttgtttag
     1081 atatttagtt gtagttttgt gacatgataa gtatgctcct ttttctcttt tccactaata
     1141 caacgatcaa agggtgcttt tttcaaaaaa ctagaaaaat caaaataaga ttcgtatttt
     1201 gattctatat atcctgtttc aactacatct tatatttcac aatagaataa atagcctttt
     1261 gcatagctgc ttaacttata agaaattgca atttacttct tcaaagtgaa aacgaatatg
     1321 agtaaaataa aattattctt cttacagata attaaaaata gcgcgtaaca aaaatcggca
     1381 ttcaatcaca taattaggat tgatgccgat tacttatatt ctacgtatag ttttagtatt
     1441 cttatttttt atcaccagta accaattgag aacagcccta aagcagaata gtcttttatc
     1501 tcaaaacgct taatttcagg cttaggaata tgaataatct tttgagtata ttctttctct
     1561 tttaatctta ctgaagaaat accactgcct cctgaataat tcatactttt aattaaaacc
     1621 atgtgaaact gcctcacggc agcttacaat tactttttca tcttcacccg ttaagtgatg
     1681 aaaaaagaac ccctttctgc tatggttaaa gtgactaaac caaccaaaag aaaggagttc
     1741 ttttcatgac tagcttacac aaaaaccaag taaaattcaa ttcaaatatc actatttctc
     1801 atacaggtgg tcaattatcg agtgattcgg gtctcgtcct agtgaaagag ctgatgaaca
     1861 tctttggttt ttctgaactg gctaagcaac acattcacat tgaagacgaa cgagcgtatt
     1921 ttactcatga caacttatcc atacttgagc agttcattat gcaattaatt gctggttact
     1981 cagctgattc tgcagctaat ctcctgagac aagatcctgt gtttaaagct gttttagata
     2041 ggaaagaact cgcttctcaa tcttcacttt cccgattttt agatcgacta tctgaggaga
     2101 atatccatga acttcaagct ttgaaccaag aacttatcga taaagcacgc ctcatccgta
     2161 atgatacgga attaatcatt gatttagatt cgacccattc agatacattt ggtcaccaag
     2221 aacaaacgaa ttataataca cactaccaaa cctatggtta ccatccattg gtagcttttg
     2281 acggattgac tggtgacttc ttaaaagctg aactacgttc aggaaatcaa tatacatcaa
     2341 aaggcgtaaa ggagtttctc acacctttat tagagcacta taatcactct ctaccaaaca
     2401 ctgacatctt ggttcgtgga gacagcgggt tcgctacacc tggtgtgtat gattcgtgtg
     2461 aatcaaaaaa gagtcattat gttattcgac tgaagaataa tcgtagacta ggtcaaatag
     2521 ctgagaaatc agtactttat ggcgataatc aaaagtggga agaacgagaa gttcagtact
     2581 tctcgaccac ttatcaagca caatcctggt cacaaagtcg tcgcgtgtgt atacgctcaa
     2641 cacgtgaagc gggcgaacta ctctttcgac atgaatttat cgtgacgaat ctatcagaaa
     2701 atgtttctcc tgataccatc ttttctatct atgccaaacg tggcacaatg gagaacttca
     2761 ttaaagaagc gaaagctggc ttttactttg acaaaacaga cagtcctcgc tttttggaga
     2821 atcatgtccg aatgatgctc agcttgatag cttacaactt agtcaacttc ttaagaacga
     2881 ttggctttga agaagtccaa aagggaatga ccattcattc tattcgattg aagtttctga
     2941 aagttgctgg gaaattagtc caaacgggta gacgagtcta tctcaaatta tctagttatc
     3001 atgtgtatca gaatgaattc tacagggtct ttgctcgcct gaggcgagcc agtcaatgga
     3061 tctaatccaa caatctaacg atttttttac tgggaagtta tccagggaga agtatgctca
     3121 aaatgcctta cacccaaaat ttattcctca atatttttga acagtgaatg tttgaagtaa
     3181 ctttttagtc aaaatcagtt gatagggata ttagtgatac atatttcaat aaaatgtgcc
     3241 aaagaattaa agctatgaat tattcaggga tcaattagaa agcaactttt ttcgggatct
     3301 aaatgaaatt ctctcttatg atccagccca tagctatgag gaactataca ctcgcttgat
     3361 tgactatgtt tacgcaaata cttttttata tcgcttattt atcctcaacc atggtgatat
     3421 ttcgtttcag aagaaagtta aacaagttat tgaagaaaac tatctgaaca tatggctata
     3481 tgaagacaag aaaacgatta tcacagaaga aatgagattc tttactacgt accacgtcca
     3541 aggttgtcta tccgttctca atcggtgggt agaaagcaat tacaagtact ccaaaaaaga
     3601 attattagaa ctcttacaga aactgaatga tcacatagag aaaatcatgc cttgaataaa
     3661 aacatactaa gcttagttaa gaagaaccgt aaccttctcg tataacaact gaatcttatt
     3721 tagccaaatt gtttactgaa gattttttga gtaaaatttc tataattcct acttattgaa
     3781 aagtagtttg tagtgctgct tctccctctc taagtgatat atcttgttgt accaatcaaa
     3841 ttcacctcat cattaataaa ttacttttag gctaatttat tacaacttat aggttgaagc
     3901 tgtaaccaat ttatagtcat attatatttt tatcatccag tttattgaat tcttctcgtt
     3961 tacctcaatt cattttcaag gggaatcagc tcatcatcta acattttacg ccatgctcgc
     4021 ttcatttctt gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg
     4081 taatccttac tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat
     4141 tgcagattag aagcatgtaa ttttttctca gaaacaaacc actcattgga aaagacatga
     4201 atcaaatcat cacttacttg tgtaaaatag cgacgtttaa tgacaaagat atcttcattg
     4261 ctatctagtg taccttcatt tctttgtgct taaattcaaa gataattttc tcaataaaga
     4321 tttttgattc cagtaagatt cctttactcc ttttacaaca tagcgttgtc ctttgttttg
     4381 aaacatcttc ttcccctctt tgccattaaa aatagcccaa ttctctata
//
LOCUS       DASJUF010000165         4425 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.171, whole genome shotgun sequence.
ACCESSION   DASJUF010000165 DASJUF010000000
VERSION     DASJUF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.171"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..1658
                     /locus_tag="U4B62_002743"
     CDS             690..1658
                     /locus_tag="U4B62_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2142841.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="U4B62_002744"
     CDS             1669..2484
                     /locus_tag="U4B62_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN2142842.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="U4B62_002745"
     CDS             2552..3262
                     /locus_tag="U4B62_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2142843.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="U4B62_002746"
     CDS             3298..>4425
                     /locus_tag="U4B62_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN2142844.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DASJUF010000166         4410 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJUF010000166 DASJUF010000000
VERSION     DASJUF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.172"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..241)
                     /locus_tag="U4B62_002747"
     CDS             complement(<1..241)
                     /locus_tag="U4B62_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142845.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKI"
     gene            complement(235..636)
                     /locus_tag="U4B62_002748"
     CDS             complement(235..636)
                     /locus_tag="U4B62_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2142846.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(823..2631)
                     /gene="uidA"
                     /locus_tag="U4B62_002749"
     CDS             complement(823..2631)
                     /gene="uidA"
                     /locus_tag="U4B62_002749"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEN2142847.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2654..>4410)
                     /locus_tag="U4B62_002750"
     CDS             complement(2654..>4410)
                     /locus_tag="U4B62_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN2142848.1"
                     /translation="VDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGSSRDK
                     NYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGT
                     EVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFG
                     QNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNSFG
                     KVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLGFN
                     GLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGIITD
                     DRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQAITLV
                     KDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKY
                     ISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWPAS
                     KGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSE
                     FKGVSPIDAYCGLKDTFI"
BASE COUNT         1379 a          881 c          558 g         1592 t
ORIGIN      
        1 taattttaaa ttttaagatc gcttctatta atttaatttc tagctgactt atcatatatt
       61 catctacata ggtaacgaat tgacctacct cgttaaatcc atttgtcaaa cataacttag
      121 ccatataaga atgataatgt ttgagtatca tcttgatcga aattggatct ccttctagag
      181 cagataagat aatggatagt ggaactaata ggtactgccg ctttttagat ttatctacca
      241 taggtcatac cccattttca attgttcaac tgccttcttt cgttgatacc agataccact
      301 tgtgctcata ttgagaattt taccgatttc agcatcatta aaacctgcaa aataataaag
      361 taggataatc tttcttctta tctccgataa ctgatcaatt gctgctgata acttttgatc
      421 tgaaattaat atttccattc caaataagaa atagacatct tgttcatcaa aatgagtttg
      481 ggaatacctg ccttcatttg aatctgccaa tggttcctca ttatccttta tgttagcgtt
      541 tcgttttcga atagacttag cttcgtttcg taatactttt ttacagtagg catcaaattg
      601 gtgttgtata gatatgtcat tgtaatgttg ttgcatgata atatgctcct ttttccccct
      661 ttccactaat acaacaatca gaaagcccct ttttttcgaa aaaactagaa aaaaaacata
      721 aaaatattta tcattcttta aacagaaaat ctttctataa aaaacaaaaa aaaacgatta
      781 ggctaggcta atcgtttttt gaacattctt aaaaaatcca aattattttt tataactgaa
      841 attgggtatt gatgaccatc gtttctgtag atagtaagca atcattttag gttttctatc
      901 tctagtaaag attcctttct tatttccttg cactcttaaa atcccttgac ttgttgcaaa
      961 atctgcaaaa ttccacactt gttcaccaac aaaattttca catgtatcta ttacttcgtg
     1021 atttgcttcg taataagcca cttgatactc ttctgtaaac attgttggta ctgtatcatg
     1081 aaatccagct actgtatctg ctccatattc tgtaaacatt attggcgtat caggacatct
     1141 ttccatccat ccatctagct cttttttcaa cagatatttt gcatgtgcca gatcccctcc
     1201 cgaaacatac caaccataat aacgatttaa acataaaaca tcgacaatat ctcctacctt
     1261 acaaacatct ggcgttgatt ttagataagt aacgattgtt acaggtcttt tctgcggatc
     1321 aagttcttta gttaattcaa ctaaaggttc aaaatattct ttagcacctt cggaatccga
     1381 atctggttca tttgcaatag accacattac aacacttggg tggttcttgt cgcgttctat
     1441 caattccttg atcaccgttt tatgagaatt ttctgttcgt atctctttcc acgtattatg
     1501 tttttccaca ttatcatcta ataaagtggc catgaaattt aaatgtaacc ctactgcagg
     1561 tacttcatca ataactacaa taccttctcg atctgctaat cgcataattt cttctgagta
     1621 aggataatgt gatgttctaa aagaattagc acctatccat ttcattaaat taaaatcttt
     1681 aatatttaca gcctcattca aaccacgtcc attaacatac gaatcctcat gcttaccaaa
     1741 tcctttaaaa taaaaaggtt tattattaat caaaaatttg ccattggtaa cctgaacact
     1801 gcgaatacca aattcttctg tataggtatc gatcacttta ttatcatcaa ttaaactaat
     1861 tttcatacta tataaataag cgtctagagg ttcccaaaaa attggattct taagctgaat
     1921 cattcctttc tttccttcag cttcagctac aagttgagcc tcttcgtcat atatcgctat
     1981 taagattttt ccggtccatt ctgtatcgat attgaattct acacttgcat ccaaatcagt
     2041 tattcttgta ttaatagtaa tatcacttat atagtctttc ggagttgtat ataacttaac
     2101 aggtctatga atacctgcat aattgaagaa atcaaaatta actaagtttt ccttttttat
     2161 atccccattt tcctgtactg tttccttcat aatccccaca ggtaaagtac tatcgtcaat
     2221 aatattgttt acagcaactg taattcgatt attcccgtct tttacaaacc gattaatttc
     2281 agactcaaat ggtgtaaacc cacctttgtg ttgagtaata aactttccat tcaaaaaaac
     2341 ttttgcttca tgtgttgcag atccaaaacg aagaactaat ctttcttcta ataatctttt
     2401 aggaagagtc acttcttttt cataccacac ccatccaaca tgatttctaa tgttctctgt
     2461 ttcaaataaa tcattataag aagagggaac tgaaatcgga taactctctt tttcatttat
     2521 tggttctaaa gcaagttctt cctgtaagtc atcatttgta tctaatttaa atctccagag
     2581 accgcttaag tcaattactt gcctcgtctc tgtcataatg ggatacaaca tataaaccac
     2641 tcctatctaa atattaaata aatgtatcct ttaaaccaca ataagcatct attggactta
     2701 ctcccttaaa ttcagaaaga cccattagtt tgtccaccaa tatttctaaa gtttcttgtt
     2761 ttccatcata agtattaata taagtcttta cttgaggtac atcagccaaa tgaaacggac
     2821 actgtacaga tacaaaaatt gttggaattt catgaatgta aaacggtata tccggagttc
     2881 ctttacttgc tggccagaca attcgttgaa ctgtgcctgg attaacatta gctatattaa
     2941 tgatcaagtc atatttttta gttaaatttt taattggtct cttagcagca tatattccag
     3001 ctatattttt aattttttct tcttgcgata atcctttcaa ttcttcttca ggtgaaatgt
     3061 atttttcaac ttcaaatcct tttgctgaca aaatctgttt caataaatca atggcttgag
     3121 gtctatttcc agcaactact tcaccaaacc caccttttac cccatgtaca tctactagta
     3181 aaatcctttt atatttttct ggtacaatag gccaaatatc ttgtttatct ttaactaatg
     3241 taattgcttg gtcagccacc tctttaaaaa ttgatttatt ttcaattaaa ccaatttttt
     3301 ccagagcttc ttgcttaggt aataaaattt gttcttttgc ttttttgtgt aatcccaatt
     3361 tagcttttaa ccctaaaatt ctttttaagg catctaataa ccgatcatca gtgataattc
     3421 ctttacgata gccttccatc atataattaa agtcttcatc tggatcatta aaaaatagaa
     3481 atagatcaca tccagctgca atagaagtgg gcaataattc tgatcgtttc attgccccag
     3541 tcattcctaa catatgacta gcatcagtaa ctactaatcc attaaatccg agttttcctc
     3601 taagtaagtc agtgattaat tcttttgata aagttgcagg aagtaaatct tcatctttca
     3661 gatcaggatt aaaatgtttt gaataagctg gcatatggat atgacctgcc ataattgaag
     3721 gtaatccagc ttctattaag cctgagtata cttttccaaa agaattatcc cattcttccg
     3781 tacctaaact attaatacta aaagatagat gctgatctcg ttcgtcaata ccgtcaccag
     3841 ggaaatgttt agctgcaggc aaaattccac tctcctgaat acctttcata taagctaaac
     3901 taagttctaa aactttatca gggttttgtc caaaagatcg actagaaatg ataggatttc
     3961 tccaatttct attaatatct acaataggag caaaactcca gttacatcca atagccgatg
     4021 cttcaatccc agaaactctc cctaattcat aagcatattt cgcatcattt gttgcaccaa
     4081 tttttacttc tacaccaact tcagtaccat ctgtacaagc tccgttacca cctgcttccg
     4141 tattagcagc aattaataga ggaattttac tttctgtttg taagatatag ttttgatcat
     4201 atacctcatc agcagttcct ggattatatc gtacagctcc aatatggtac tgattgacca
     4261 tattactcaa gtaattttta tcacgactag atcccatatt aacaaacaat tgacctattt
     4321 tttcttccaa agtcatagaa tcaatcgtct cttcaatcca agaaatagca tcttcatcta
     4381 aattatatgg tttttttcgt aaatctactt
//
LOCUS       DASJUF010000167         4328 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJUF010000167 DASJUF010000000
VERSION     DASJUF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.173"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..1372
                     /locus_tag="U4B62_002751"
     CDS             join(33..474,474..1372)
                     /locus_tag="U4B62_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN2142849.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            1484..2380
                     /locus_tag="U4B62_002752"
     CDS             1484..2380
                     /locus_tag="U4B62_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2142850.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            2478..2687
                     /locus_tag="U4B62_002753"
     CDS             2478..2687
                     /locus_tag="U4B62_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN2142851.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2705..2977
                     /locus_tag="U4B62_002754"
     CDS             2705..2977
                     /locus_tag="U4B62_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2142852.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            2979..3842
                     /locus_tag="U4B62_002755"
     CDS             2979..3842
                     /locus_tag="U4B62_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEN2142853.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1743 a          645 c          754 g         1186 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttcataaag agggctttta tatattaatc acaaatcact tatcacaaat
     1441 cacaagtgat taatcacaaa tcacttgtga tttgtgattc ttaatgatac aatattacta
     1501 tacaaaaaaa gaatggggcg tagttatgga gaaggaaaaa ctaaaaatac ttgaagaatt
     1561 aagacgtatt ttaaacaata aaaatgaagc aattattatc ttgaataatt actttaaagg
     1621 tggtgttgga aagtccaaat tatcgactat gtttgcttac ttgacagaca aattgaattt
     1681 aaaagtttta atgatcgata aggacttaca ggcaacattg acaaaagact tagcaaaaac
     1741 ttttgaggta gaattgccac gtgtcaattt ttatgaaggc ttgaaaaatg gaaacttggc
     1801 ttcttctatt gttcatttga ctgataattt agacttgatc cctggcacgt ttgatttgat
     1861 gttactgcca aaattaactc gctcatggac ttttgaaaat gaaagtagat tgcttgctac
     1921 tcttttagca cctttaaaaa gtgactatga tctcattatt attgatactg taccaacgcc
     1981 aagcgtttat acaaataatg caatcgtggc gagtgattac gttatgatcc ctttacaagc
     2041 agaagaagaa agtacaaaca acattcaaaa ctatatttcc tatttgattg atttacaaga
     2101 acaatttaac cctggactag atatgatcgg ttttgttcct tatttagttg atacggacag
     2161 cgcaacgata aaatcaaacc tggaagaact gtacaagcaa cataaagaag ataacttggt
     2221 tttccaaaat attatcaagc gaagtaataa agtaagtacc tggtctaaaa atggaattac
     2281 agaacacaaa ggctatgaca aaaaagtttt atccatgtat gagaacgtat tttttgaaat
     2341 gcttgagcga attattcaat tagaaaacga aaaagaatag aatcacaaat cacaagtgat
     2401 taatcacaaa tcacttgtga tttgtgattg ttgatgataa aataagaata agaagaaata
     2461 gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agcaaaacga aatgatacac
     2521 caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt gctgacttac
     2581 accatattat aaaaattgaa acagcaaaaa atggcggaaa cgtaaaagaa gttatggatc
     2641 aagccttaga agaatatata cggaaatatt tacctgataa actttaaaaa ggagtgaaaa
     2701 tgaaatggca gttacgtatg aaaaaacatt tgaaatagag atcattaacg aattatcggc
     2761 aagcgtttat aatcgagtat taaactatgt tttgaaccat gaactagata ctaaaaatac
     2821 tcgtttacta gaagtgaatc ttttaaatca attagaagtg gcacaagaag ttgatttatt
     2881 tcaacaacca tttgaagaat tacaagctat tcatgagtat tggcggtcaa tgaatcaata
     2941 ttcaaaacaa attttgacaa aagagaaagt ggcttaacat ggcgaatatt actgacttca
     3001 ccgaaaagca atttgaagat cgtttagaaa agaatgttga acgactaact aaaaatagac
     3061 tagcggttga atcgccaacc gcttttttac ttggtgggca accaggatca ggtaaaacta
     3121 gtttgcgatc ggcaatttcc gaagagacac aaggaaatgt tgttatcatt gataatgata
     3181 cgttcaaaca acagcaccct aattttgatg aactagtgaa actttatgaa aaagacgtag
     3241 taaaatacgt taccccttat tctaatcgca tgacagaagc gatcataagc cgtttgagag
     3301 ataaagggta taatttagtg attgaaggta caggacgaac aacagacgtt cctattcaaa
     3361 ccgcaacaat gcttcaagcc aaagattatg aaacaaaaat gtatgtcatg gcagtaccta
     3421 aaatcaactc atatttagga acaattgaac ggtatgaaac tatgtatgca gatgatccaa
     3481 tgacagccag ggcaacacca aaacaagcgc atgatattgt tgtcaaaaac ttaccgacca
     3541 atttagaaac ccttcataaa acgggcttat ttagcgatat aaggctttac aatagagaag
     3601 gagtgaaact ctattcaagc ttagaaacgc cttccattag tccgaaagag accttagaaa
     3661 gagaattaaa tcgtaaagta tcagggaaag aaattcaacc gactttggag agaatagagc
     3721 aaaaaatggt tcaaaatcaa caccaagaaa cccctgaatt taaagcaatt caacaaaaaa
     3781 tggaaagctt gcagccacct acaccaccaa tacccaaaac acctaaactt ccagggattt
     3841 aaaccttaaa aaagtaaaag agtagttacc aaaaaacggt aactactctt tttttataaa
     3901 aacatttcct cattttttaa aagctgatcg agaaataaag cacacaactg tattctttct
     3961 tttttcagtt ggtacatttt gcagtctgta acgtgccatt tactgtttgt taccttaaca
     4021 tatccagtcg aaaacacaag ggtatataaa cgagcaagct cgcccttgtg ttttctttgt
     4081 gtctatgttg cgttctcgtc acagcaaacg acacgaacac actggcgaaa atgaaccaac
     4141 aaaacgaaaa aagaaaggag cgtacactgt aatttatttc tagcttttaa aacatgagga
     4201 aaacagcttc acttcctcaa acgcctaaca aaaatggttg tcaaaaaact aaaaatcaaa
     4261 aaaagaaaag aggaaaagaa aatggaaaaa acaaaaaaat tacaattaga agattttaca
     4321 gaaaatgg
//
LOCUS       DASJUF010000168         4295 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJUF010000168 DASJUF010000000
VERSION     DASJUF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.174"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..790
                     /locus_tag="U4B62_002756"
     CDS             <1..790
                     /locus_tag="U4B62_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2142854.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            887..3136
                     /locus_tag="U4B62_002757"
     CDS             887..3136
                     /locus_tag="U4B62_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2142855.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3139..>4295
                     /locus_tag="U4B62_002758"
     CDS             3139..>4295
                     /locus_tag="U4B62_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2142856.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1501 a          610 c          791 g         1393 t
ORIGIN      
        1 ttgtgggtat tcaaaaacag aacctagaca tagttttgga ccagctattc gagaacaata
       61 cttaatacac atagtgctag aaggtgaagg ctattattca attaatgatc aaaaatattt
      121 tctgaagaaa ggccaaggat ttgttatccc accaggagta tctacttttt atcaagcaga
      181 tgaaaaaaat ccgtggtcat atgtatggtt gggtttaagt ggcaatcttg tagaatacta
      241 tctaaaagaa attggtgttg gaaagaatca tcttaccttt aaagtcgaaa atttaaattc
      301 atttaaagga ttaatttttg aaactttatc gtatgatcag gacaatctag tcagtgaatt
      361 gtccagacaa aaacaagttt ataaattttt agaactcctc ttaaattcag ctagttatct
      421 gaacgaaggg ataattacta aaaagatgaa tccttttgtt cgtcaaacaa ttgaaataat
      481 acgtgagaat gcgtatcaaa aattatctgt taaagatatt gctgtcagtc tatcgataca
      541 cgaaaattat ttatcacgat tgtttaagaa tgatatggga atatcaataa aagagtatat
      601 tagtgaaatc aaaatttcta ttgcaaacga tctgttatca tcaactgaat attcaatcca
      661 gcaaatcagt gacaagatag gattctcaac agtacaatct ttttctaaag cctttaagaa
      721 agcaaatggt gtttcaccgg gagtatatcg aaaacgtaga ttaggtctag gaatcattaa
      781 tggtgaataa actaaacatt tgattttgat aaatattcgt tactttaaac gcttactatt
      841 tatgtgagcg ttttttataa tgcaataaag aaacggagga gagcttatgt ataaggataa
      901 tgaaatgaat attatttatc gatcagaaaa ccaacaattt catttgttta atcaggagat
      961 aagttacatt tttaagatat cggaagatgg taaattattg catttatatt atggtgagaa
     1021 cttaccagat aatgactata gtcatctaat tgagatgcat catcggccta tgacaaccta
     1081 tagaaatgaa aacgatttgc tatattcgtt agagcactta aaacaagaat ttcctgaata
     1141 tggtacaaca gattttagac atcctgctat ttcattacta cagaaaaatg gctctcgctt
     1201 atcagatttt gtctatcaag ggtacgaagt ggaagaagga aaacctaaac tagaaggttt
     1261 acctgctacc tatgttgata ataatgatga aagcaaaaca ttgaaagttt atattttaga
     1321 ttcagttaca aatgttcaag tagaattatt atatacaatt tatagagatt atcctgttat
     1381 tactcgtagc gcgcgagtta tgaataaagg aaaagatatt caaataatcg agaatattag
     1441 cagtttatca ttggatttac ccgatgcaaa ttatgaatgg caacaattat ctggtgcttg
     1501 gggtagagaa agaactatta agtcgcgtac tttacaacaa ggaattcaat caatcgaatc
     1561 tactcgaggt atttcaagcc atcagcataa cccatttgtt actttaaaaa gaaaaaatac
     1621 tgatgaaaat caaggagaag ctattggtgc agcactagtt tactcaggaa acttcttaat
     1681 tcaagcagaa gtagatacat gggatgttac tagactacaa gttggcatca atccatttgg
     1741 atttaattgg aaactcaatc caggagaaat atttacagca ccagaagcga ttctggttta
     1801 ttcaagtaag ggtttaaatg ccatgagtca aacttttcat caactattta gaaaacgctt
     1861 agctcgtggt aagtggcgag aaaaagaccg tcctgtctta attaataatt gggaagcaac
     1921 atattttgat tttgacgaag aaaagttggt aaatattgct aaaaaagcat ttgatgttgg
     1981 tattgaatta tttgtattag atgatggctg gtttggagaa agagaaaacg attgtgcagg
     2041 attaggagat tggcatgtaa atcctaaaag actacctaga ggaatcggca gcctttcaga
     2101 aaaaattcgt agttttggta tgaaatttgg tttatggttt gaacctgaaa tggtgaataa
     2161 ggatagtgag ttatatcgca cacatccgaa ttggattatt tctacaccta atcgaaaacc
     2221 taatcatggt aggaaccaat atgtattaaa ttttgggtta gatgaggttg ttgaaaatat
     2281 tttcgatcaa atgtgtaaaa tcattgatga atcaaactta gattacatta agtgggatat
     2341 gaatcgtcca ttgactgatg tatttgacag tcacttgtca gcagatcaac aaggagaagt
     2401 attccacaga tatgtactag gcgtatatcg cttatatgaa aaacttataa caaaatatcc
     2461 taacatacta tttgagtctt gttcatcagg tggtgggcgc tttgatcctg gaatgcttca
     2521 ctatgcacct caagcttggg ctagtgatga ttcagatgca attgaaaggt taaaaattca
     2581 atatggaact agtatgctgt atcctctttc gtctattggt gctcatgtat caattgttcc
     2641 aaatcatcaa acaaaccgta taacacctat aaaaactaga ggaaatgtag ctttttttgg
     2701 tgcatttgga tatgagcttg acttaaatga attaagtgaa ggcgatttga ggattgtaaa
     2761 agaacaaatt gccttttata aaaaatatag aaccataatt cataatggta ctttttatag
     2821 actattatca ccattcgatg atgataatca aaccgcgtgg atggttgtta gtcatgataa
     2881 gaaaacggct attgttgctc attataaaac attaaatgag gtaaatgctc cttaccgccg
     2941 actgaaatta aaagggttag ataatgacgg actttataat gtaggacaaa atagtttgcg
     3001 atctggaatg gaattgatga gagcaggact agtctgttca gatgcatcta gtggacaaat
     3061 aaatgatcag aatcgacgcc cagaaacatt tgattttgat tccaagattt gggttataac
     3121 acaggaggaa aagtagagat gaataaccaa aaaagcttac ttcaaaaagg agcaatctta
     3181 agtatttcac taatgttaac aagctctcaa gcaattaatg gtgttattcc acaaatgaaa
     3241 cagtctttgg ggatatcaca aagtcaaagt gaacttttag gaactgcacc aagtataact
     3301 gttattttgt ttatcttatt atctagttac tttgccaaaa gaataggaat gaaaaagaca
     3361 ataattttag gtttattatt agctggtata ggaggaatta ttccagtatt tacagctacc
     3421 ttccaaaccg ttcttattag tcgagtaata ttaggtgccg gtttaggagt atataattca
     3481 ttggctgtga catatatcag ctctttatac tctggcaata ctagagcaac gctattaggt
     3541 attagaaatt caatggaagc tttaggtcag acaatcctta tttttcttgc tggagtttta
     3601 gtgaacatta attggacggc ttcgttttta gtctatgcaa ttgcgtttcc tgtagctctc
     3661 ttattcgcgc taaaagttcc tgaaattaga gatgaaaata gtgacatacc tgaaaatcat
     3721 gtaaaagaaa agatgaatcc aatggttttt gccctagttc tatttgcaat cttactcgta
     3781 atgaatagta ttgctatttc tgtacgattt gcatcaattg caactgaaat taaaggggta
     3841 aactttaatg ctagtaacta tttagctcta atgcctatct taggaatact agcaggattt
     3901 atgtttggac caataaataa gtggatagga aaaggaaccc tatacttggg aattatattc
     3961 tttataattt caaatttgtt aattgctgtg tcaaacggta atatgacgtt cctgctaacg
     4021 ggtttatttt tatcaagtat aacaggatca tggtgcttcc catttatctt tagtaactta
     4081 gataaagtca ctacaaagaa tacaattaat tttgctactt cattgatttt tattggctgt
     4141 aatattggta attttatagc tcctattgct atgcaattag cacaatttct aacaagaagt
     4201 acaaatttga ctgctccatt ttttgtattc gcaggaatat ttattgtgat cctatttgta
     4261 acattttttg cgacaaataa aaaaaatact agagt
//
LOCUS       DASJUF010000169         4283 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJUF010000169 DASJUF010000000
VERSION     DASJUF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.175"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..988)
                     /locus_tag="U4B62_002759"
     CDS             complement(<1..988)
                     /locus_tag="U4B62_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2142857.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTI"
     gene            complement(1096..3123)
                     /locus_tag="U4B62_002760"
     CDS             complement(1096..3123)
                     /locus_tag="U4B62_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2142858.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3268..3999)
                     /locus_tag="U4B62_002761"
     CDS             complement(3268..3999)
                     /locus_tag="U4B62_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2142859.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1181 a          893 c          711 g         1498 t
ORIGIN      
        1 taatcgtaac atccgtcgtg ttcaattcct tggctacttt tccgtctatt gtaaaatctt
       61 tttcaaatac ccattgattg gtatagttga tcgtagtatt tccttttttt ctcgaaaatt
      121 ttccattttc attttcattt cctaaaacag tcattgtaag gtgatattca cctggggtta
      181 gcggttgacc atttaaagca gttggaaaag aaaaatttgt attcggcgca atctgcaatc
      241 cttctttttc ctcttgatat aaaacttcag aatgtccttt ttttgtaatt tttgtttcaa
      301 taactacttg attgatatag gttttttgat cattttgaac gtttgctaag atgacatttc
      361 ttgcattgat ttgatctggt ttaacttcat gaagagtaag gttcggagca acttcgttca
      421 gtgtctgttg catcaacaaa gcaataatat aggtgtattc attctcaatc gataagcttt
      481 gatccttagt ttctgtttgg tccttttccg tctggatttc ttggaatgtg atcccaccag
      541 ccaatacacc atcaaatcgc tcattcggca tcctgacatg aaaagcaacg gtttggacac
      601 ttttaggttt tagcgtcact atctgtggat agctcacata atctaccaga tcaaagatca
      661 ggctcatgtc tttttcaatc tcgttttttc cgtattcaac catcacattt gaattggtgg
      721 ttgcgctatt cacagaaata tcgattttca cttctttttc tgtatcattt ctcaattccg
      781 cttttacttc tacttcctga tccggatcaa gttgaagatc gaagtaagtt tttctcttgt
      841 cgatttgatt ctctgaagtt atcggcgtca ctgaaaaatt aaatgcacta gctgacactg
      901 atatattgtt aaaaagtgcc cctaaaataa ctaggagtgt tataaataat tgttgttttg
      961 gtctcaagaa aatcgctcct ttaatcatat gtaagagaag actataacaa aatcttgtct
     1021 ctgtgggaca caagagccta tggtacctgt gcccttcttg aaactcaata attttgttat
     1081 attcttctct tcctgttaat ttcctgggac atctgcaagt atccaagata gtttcccctc
     1141 aaaggttccg attctttggt tttctacagg aatatttatt ctgattccac gatgagctgt
     1201 agaatctaaa agagaggaca aatcgctttc acccttttga tcactttcga aaatcgtaac
     1261 agcggatgtc cctataaccg ttgtattttc agaggtaaca aaggaaagag cttctggaag
     1321 ctcaaaatct ccatttgtaa gaactttctc ttgtttcaat tgtaatttcc aacttccaaa
     1381 agcccgttca tctataattt tcaacccacc tgaaaactgt ccgaattcac ttaatggtct
     1441 actggatatt ttatgttttc caaaattgac atattgagga gcagttgaga gataaagttt
     1501 gccttttctg taataaaaat agatatcctc tgtattttcg gagaagatgc cttctgtatt
     1561 cgctgaatcc gagtcaaaaa catacccttc aatatcaata ggagtttctt tatacttctc
     1621 tcctattttc ccagtgattg ttttcatttc agcaatatca atctgactgt ctttatcttt
     1681 ataataaaca tggatatcta tctccttcag ctttgcgatc tttaggttta tttcattcga
     1741 ttgctcaatc aaattcccgt tttcaaggta ttcaaaatgc atagtgagta ctttttcagg
     1801 aattacttcc tgcaaagttg tctccaattc gattattaac gtatctccag caataaaata
     1861 atctgtcaga tttagcgtga ctcgatttcc ggttatcaag atatctttgg catcaatttg
     1921 atttccattg atcaagatat gctcgggaat agttaattct tgtgggaggt cgatactagt
     1981 ctgcattttc ttatattcaa gaaaatcccc ctcgaccttc aatgtatttc tgatagttat
     2041 cttatcgttt cctccagctt cagcgatggc cccataatca ctatcgttga cttttacatc
     2101 agctgttgat gtgacattcg atggaagtcg cgtgacagcg acagcatttt cttgaaaaaa
     2161 agtacctgtt gaagaagtaa acccccaatt tacttctgta gaaccaaact gcctaatgag
     2221 atcaggtata atccgagaag agcgaccgac accttccaaa tcataagtaa gacttctttg
     2281 ctccgcgtcc catttaatta ttaacaagcg ccatgtgcca ttagaaagaa atgtaggagc
     2341 taccgtcacg ccagtatgct ctccagaata attattatta tttggtttag gcgtgacaaa
     2401 agcgacatgt ccatgccctc tattatcagc agttatctct cgatccattc gatcagaaga
     2461 accactattt ttatacgtat caaactcaat cgagagcgca ttcgcaatgt aaggttggcc
     2521 agaacgagta gaatatgagc caattcccat acctggcgat ccaataactt gactgggatt
     2581 actagccatt ctaggatcgc cagtcaatgt aaaggtcatg ccatctcccg ctctatctcg
     2641 ttgatttcct aagtataaat aagatttaaa tgtaaaatcc gagagtaaac taatcttctt
     2701 atttgaccaa atagctcctc tttgagacct ttctgctggg ttcaactgca gtaacttccc
     2761 attttccaat atggacgtgc tagtaccgat aggtgtttga aaaattttat cgatagaaat
     2821 aatattggga tcgtcaggca gctcaaccgc agaaattggt gcatcttcta gtccttgttt
     2881 gtccgaaccc tcagcttcta cgttgtcagt ggtctctcca ctactcgaaa tacttaaatc
     2941 ctcactgctt tcttgtgtct ggttggattc tattaaactg tcgaatgtcg aagtgagttt
     3001 gctttcttcg tctcttctat ttccatgctc caactgattc aattccaaag agtaccctat
     3061 gatcggaaag atattagtca ctaagagact gcttaaaatg agcacgatcc tcctcttttt
     3121 caatatatct tttcctcctg atcgttaaat tcctagatga actattcgta tccaaacaaa
     3181 tttagcctag cctttgacaa tttttgctcg attacctcgc aagacaatat gactacgtat
     3241 ttatttttaa cagttctttt cagtaaacta gttgttaggt gtatcttcta atgtccaaac
     3301 taatttagtg gaatattgtt gcgctaactt gacagattta ccgggtaccg aaagctgtac
     3361 cgcatcttga ttttcatttt catttgcacc aaatcgatag acccaagtcc ccacacctgt
     3421 tcctgattta gcattaattg ctaacgtatt gtttcctccc ggtgtcaacg ttaattcaga
     3481 attcaccgtt tgcggcgtat atgttggatc aaccaaagaa acagcctgcc ctttcgtaaa
     3541 tttcagctgt gctccaacta gttcatcccc actggcagtt ttaaactgtt ctggttgaga
     3601 gacacttaat ttccacccag ataaggtact tctcctatct gtcacttgga catatgtcgg
     3661 ttttctagac ccatcgttca tcacatcagc agctgcataa tacgttttat ccgcagtaga
     3721 aattttttga gtcccaaatt tgaattttga tccataatca atcgataatg cccctcctgt
     3781 tccagggctt ggaggatctg atggatcgac aggtttattg ggatcaggat tctctggatc
     3841 aacaggagga gttacaccct catctcctgc ttcaaatgta atcgttcctt ctgtgttata
     3901 ttcctttggg tatacttcag ttgcagaaac aaaagtcgta cttgctacca tgctgaataa
     3961 catcattgct gctgttaata cgatttcttt tttcatcatt gacattcctc tttctttatt
     4021 tactaaacat atcagtcgat tggattaatc catagcaaaa ttctgtttta cgatacttat
     4081 gaaataaaag aacatagacc tatatttggt cctatgtatc gattagttat aaatgattat
     4141 cagcccgatt tgatttctag tttttattgc aacttgatgc aaacacaatc tagaaataca
     4201 gctgtcatag cctttaaaat aattcgtaag tccccctcct atctatcaaa atgtgacttg
     4261 acgagagtgt aaaatatttt gtg
//
LOCUS       DASJUF010000170         4282 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJUF010000170 DASJUF010000000
VERSION     DASJUF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.176"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="U4B62_002762"
     CDS             complement(37..1257)
                     /locus_tag="U4B62_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN2142860.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="U4B62_002763"
     CDS             complement(1277..1888)
                     /locus_tag="U4B62_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2142861.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="U4B62_002764"
     CDS             complement(1931..2863)
                     /locus_tag="U4B62_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN2142862.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="U4B62_002765"
     CDS             complement(3284..4144)
                     /locus_tag="U4B62_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142863.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DASJUF010000171         4272 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.177, whole genome shotgun sequence.
ACCESSION   DASJUF010000171 DASJUF010000000
VERSION     DASJUF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.177"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..503
                     /locus_tag="U4B62_002766"
     CDS             75..503
                     /locus_tag="U4B62_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2142864.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="U4B62_002767"
     CDS             490..732
                     /locus_tag="U4B62_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142865.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="U4B62_002768"
     CDS             complement(1032..1247)
                     /locus_tag="U4B62_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142866.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="U4B62_002769"
     CDS             1354..1668
                     /locus_tag="U4B62_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142867.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="U4B62_002770"
     CDS             1772..2950
                     /locus_tag="U4B62_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2142868.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="U4B62_002771"
     CDS             3353..3646
                     /locus_tag="U4B62_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN2142869.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1459 a          645 c          823 g         1345 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
     4261 aagcaatact tc
//
LOCUS       DASJUF010000172         4248 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJUF010000172 DASJUF010000000
VERSION     DASJUF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.178"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            48..485
                     /locus_tag="U4B62_002772"
     CDS             48..485
                     /locus_tag="U4B62_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142870.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            580..1464
                     /locus_tag="U4B62_002773"
     CDS             580..1464
                     /locus_tag="U4B62_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2142871.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1686..>4248
                     /locus_tag="U4B62_002774"
     CDS             1686..>4248
                     /locus_tag="U4B62_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142872.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1391 a          713 c          959 g         1185 t
ORIGIN      
        1 cttaaataaa gaaaacgata ttaaaaaatg cacgtagaag tagataaatg gagtggtttt
       61 gcaaatttct tttatttcta gaagttattc acgtatacgg atttgttttt ctcacaaata
      121 aaaaggcagg aagtacaatg gatcatttgt atataaaata tattcatcaa ttttccgttt
      181 tggtattagg cacactaatt atagcgggga ttgttgcatt tattattcaa ggtcgtgaca
      241 tattaacagt acatcaagag tttttcagtt atgcatttgg tattcctctg atcgctataa
      301 ggtctgataa caaaaagtgg aagaaacgct attacattct cttgctattt ttgtgttttt
      361 atctgccacc taagattttt actttattag gtatacaatg cctatcattt tcttttattt
      421 cctatatcat tgttttaatt ttcctcagtt tcatagtatg ggtattttat cttgtcaaaa
      481 aataaatgaa tgtttcatga acagtacttc atcacagcct aatttttttc tttatgctat
      541 cgttatatta aagaaatcaa tcagaaggga aagaaagtga tgaaaaaagt tttattagtg
      601 gtaaacccca gttcaggcgg tgaacaagca aaggaatttg agcaattagc gatagcaaaa
      661 ttggaatcgg tgtttgatga agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa
      721 aactttactc gcgaagcggc tacggaaggg tatcatagtg tgtttgtcat gggcggagat
      781 ggaacagtca acgaaggaat cagtggaatc gctgaacaag aacatcggcc aaatttcgga
      841 tttttcccat tgggtacggt gaatgacttg gctcgagcat tagggatacc gctagaacct
      901 gaggaagcga tcaatcattt ttcgattgaa tcagtcaagc cattggatat tggaaaaata
      961 aatgacgact attttatgaa tgtcgtcgca attggctcta tccctgaagc aatcaatgat
     1021 gtagatgcgg agaagaagac gaaatttgga aaactagctt atttcatgtc aggaatcaaa
     1081 cagttagctt ccacacaatc ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa
     1141 attgagagca gtatcttgct gataggacta actaactcag taggcggttt tgaaacatta
     1201 cttccaaatg caaaagtgga tgatggtaag ctccatttgg tttatttaaa agattcttct
     1261 ttgatcgata cgcttaaaac attgccagac ctattaaaag gagtagatga atctacgaaa
     1321 aatctagcct atcttacttg tgagcaatta gttgtgtcat tagctgatga ggaagaattg
     1381 acgacgaatg ttgatggaga tgaaggggat aaattacctg tgacaatcaa agtgttaccc
     1441 tcacatttac aggtttattg ttaaaatgga ggttgtgacc gaagagttgt caggaggaca
     1501 tcggttgttc ggaataaggg actgtgacaa gacttttgtc acagcctttt tttgtacaaa
     1561 aaagaaattt tcacctaaca gaaaaatttg ccataaaaat acatatttta aatatgaaag
     1621 tacaattaca aataaattaa aacgttaaaa taaaattgtc aaaacgtcag agaggatggt
     1681 ggagaatgaa tcaatatgta aaaaagctga ttgcgtcgat tttgtttgtc ttccctctct
     1741 tgatggtgac atccgttttt gccgatgtag agaaaacaag cgaatcaacg caatcagaga
     1801 atcaaagttt tgacaagact actttttcag aagaatcaat tacttctgaa actacagaat
     1861 cccaaagtga ggcgcctact tctaatacaa ggcaagaaga aaataagaca agtgagacct
     1921 cagaagtagc tgaacaaacg tctgatagca gtgaagaggt acaggttaga gttgtttcag
     1981 cggatgggta cagcgaagct gccacaacag aagaactgac gcagcttctc gcagacgaat
     2041 cagtagctaa gattcgttta actcagcctt tgactttaga cagggaattg gagatcaagc
     2101 gagatatcgt tatcgatttt ggcggacttg ctcataattt cggcacacat cacatctata
     2161 tcaatgagac acctcagtcg atcgagtttc agaattttaa aggaacggca gctcatccag
     2221 gtgggttgat accaaatatc gatggaaatg ccattatcat tgcttatatg tccgattttt
     2281 ggggaaacag atatcatttt acgggagaaa taaaatttac cgggacactt gacttatatg
     2341 atggttctaa attaggcctg atttacgccc ctcgtgcaac tgtcacatta gatggtgttt
     2401 ccggtgtatt ggatgtccaa ccagtgaaag aagggatgaa tgccgctccg ggtaaagctt
     2461 atttttgtag aagctatcag ctaaatgtaa tcaatggtag tcagctttat ggaccatatc
     2521 taggaaaatt ctatggtttt ctagattcag gtgatgagac gggcagtgga aaaacagcac
     2581 caggactcca tatcttgagt ggttcaaagg tctctcttga ctatgatcgg gcagatggcg
     2641 tcagcgatga aggagaggct gtcgatacgc tacccgggaa tgtcacattt aaagtcagtg
     2701 gctcaggaag tgaattttcg gtaaatacaa agatcaatat taccaacgac aacaatcgcg
     2761 gaattatcca aatgagaggc tcaggatcaa gagtcgctgt gtcgaatgat gggaaaatca
     2821 caatcaatac agcgactact ggcggatttc gcctgcaagg agattctagt caaatccatg
     2881 tatcaagtgg tggacaaatc tcggtcaaaa tggctggaga tggcgaccaa cctggaaaca
     2941 atgggatgcg ttttgtaggg aaaggacttt ctttgatcgt agaaggagcc ggatcagtca
     3001 tcgatattga taagcaaagt ggtcgttcta gcgcagttcg ttttgagaac ggtacgcaaa
     3061 aactaacagt aactaatgga ggctcattga aggtaagaaa tgcaggggat ggcaaatctt
     3121 atgtgaacag actcaatcaa ggaaaccaag cgatccaatt ttatgacgct tctggttttc
     3181 tagaaagtcc agggtcagct gattttacgg ttactggaga aaaatcaaat atcgatatac
     3241 gcgcagattc cggagctacc gtagatacaa ctggagatat tgatttgaac tttatggctg
     3301 gcgaaaaaac ttatttagtc atggtcggga aaactgggga agataagact ggcatcatca
     3361 gtggggatgt gttgaatgta gacttagaat ctcctaccta tttcgatttc caaaacaaac
     3421 ggatcggaag cgagactaat ggcataggtg gctgggtatt tcaaggaaat ggcgattctt
     3481 ctttgaagat cgctcattca gaaattgctt tatggcgaaa ggcaggagta acgttcgata
     3541 actttgaaaa agatccggat tatttttctg atatgacaga cttatcgatc aatggaaccg
     3601 acttaggtaa ttttgtatca agccctagtg caggtttaac aaatgaattt gctcagacag
     3661 gtgccggaat gaaaaattat aatcgaatca gtgccaataa ccagcttccg atgattgaat
     3721 ccttaagagt accaacgaat gcagataaaa aaatctatgg gcatgtcgtt gttcccgaag
     3781 gtgtcgaggc aaagccaaga gatgcttgga caggcgaagt agaagtgact ttacgtatta
     3841 cttatgcaga tacgacgaaa tcacctgtag aaattactgc tctcacacaa ggacaaacag
     3901 atgaaaatga agggacagga tataatccgt ggggagaagg cgaacaaggc ggtttgtttg
     3961 aggttcaagt acccaacgaa gaaagactaa caacggggga tcaagtgcaa atagttgctg
     4021 ctaaaaaagt aagaggcggc caagaagtga cagaactgga ctctgccaaa acgacagtcg
     4081 atgtgattcc tcctgagcct gctaaattaa gtaccacatt gatcaatgca gcttctcgct
     4141 caatcacagg tacaggcgaa gcaggagctt tggtttcatt gaaaaaaaat caagctgttt
     4201 tgtccgaaac aactattgat gatacaggta aatttgttct ttctatcc
//
LOCUS       DASJUF010000173         4200 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJUF010000173 DASJUF010000000
VERSION     DASJUF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.180"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..155
                     /locus_tag="U4B62_002775"
     CDS             <1..155
                     /locus_tag="U4B62_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142873.1"
                     /translation="KNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIRRVIGC
                     GGLEFF"
     gene            155..526
                     /locus_tag="U4B62_002776"
     CDS             155..526
                     /locus_tag="U4B62_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142874.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            519..914
                     /locus_tag="U4B62_002777"
     CDS             519..914
                     /locus_tag="U4B62_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2142875.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            916..1293
                     /locus_tag="U4B62_002778"
     CDS             916..1293
                     /locus_tag="U4B62_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142876.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            1294..1902
                     /locus_tag="U4B62_002779"
     CDS             1294..1902
                     /locus_tag="U4B62_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2142877.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            1902..2243
                     /locus_tag="U4B62_002780"
     CDS             1902..2243
                     /locus_tag="U4B62_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142878.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            2485..>4200
                     /locus_tag="U4B62_002781"
     CDS             2485..>4200
                     /locus_tag="U4B62_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN2142879.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLF"
BASE COUNT         1484 a          549 c          872 g         1295 t
ORIGIN      
        1 taaaaaatga atcagaaaaa acagtatctt cagttagacg tggcgataca acgattagtt
       61 atgcttcaac aacaaatgat gttagtgagt tacttctagg ctatggtgat ttgatacgaa
      121 gagttattgg ttgtggagga ttggagtttt tttaatgaat gaagcagata ttttagaaac
      181 tacttatgaa gatagttgta ttattgaaag actgacggac attgaagatt ctaatacaaa
      241 tattactatt caagattaca aaaaagtata tgataatcct atttcttgcg ctctttcaca
      301 aggtcaaatc gatggactag cagtcataga agatggagag atggtaaatg tttcaactga
      361 cacatataaa ttatttgttc atcctaagat taaactaaaa aaaggagatc gaataacaat
      421 aactcaaaag gccagtggct taattttttc tctatttgct actaagcctt tttactatcc
      481 tagtcattgt gaagtgaatt tgataggaag tgagaaaaat gggtaatctc aaatatgaat
      541 caaatgcaga aaagatcatt gagaatttta aaaatatgac tgtaattgct caaaaagaag
      601 gaatatcctt cgttaacgat tcaatgaata aagtcgttag tttagctaaa ccattaactc
      661 ctgtaaaatc aggtaattta aggcgaggat atcgtgtagt aaaagctaga aaactatcaa
      721 gcggtcgaat tgttggagaa gttataaaca atgaacatta ttttaaatat gtagaagaag
      781 gtcataggac taaaaatggt ggctttgtca aagggaaatt catgttaact cgtgcaacga
      841 atcttgcaaa tatgtcttat attcctcgaa gatttaaaca aatggcaata aaaatcgtta
      901 agaaaggaaa gtaacatgaa agatgaaatc attgctgcaa tcagtagcaa attaaaagaa
      961 atctatcctg atgggacaat atatcttgat tcagttatgc agtcaactaa agatttttat
     1021 ttcgttttat cagtaatgga atctggaact gaaaatgtag gaattgatat tcaaaatgtt
     1081 tctttcttaa ttgatattgc attgattgat aataacccta atagaaattt aattaatgaa
     1141 ttagtctcac gttgtgggac tttttttaat acgattacaa tcgatgagca aatattattt
     1201 ccaaaagcct atttacctga tgaagcagat ggtgttcaac atattcgttt tacattagaa
     1261 tttccacaat atattgaatg gagtgaaaga taaatgggag aaaaaagaag taaaaccgga
     1321 attatttctg tcgaaaaacc tacttggttt cctttggaag atgagacagg gaattttccg
     1381 gtatatggta ctgcgttgcc aatgggaaca gcagtaagta tcaaaccaac agctaattat
     1441 gaaacaacac aagattatgg ggattcagtg gttcaagatc aatttacagc ttttggtggt
     1501 gcagaagttg aactggaagc aaatggatat actcataaag ttttatctac aattactgga
     1561 gggaaaattg ttaaaggtgg tgccttgcgt tctggagaag atattgcgca agatggtgcg
     1621 tttgcatata gacgtaaaaa atcgaatggg aaataccgtt atacggtctt ttataaagga
     1681 cagtttgctc tagattcaga tgaaacatca acgattgaag gaagtaaagt aagttttact
     1741 catccaactt ggaagggttc atttgtagat gtaccaggac ttggatatat gtattcagtt
     1801 gatgaagatg atgaaggcgt ggataaagca atgatcggaa attggtttac aaaagtagca
     1861 attccaattg aagaagcaga actttcagga ggtacagaat aatgtcaaaa tatcaaacaa
     1921 cgattaaatt aacgaaaaaa gacgaagaag gtaaatatga gcaagtacaa tttaaatctg
     1981 ctgaattttt accaggaaca gttgtagaag atgctgcagg agttatggaa gaaatgcaaa
     2041 cagcgactga taagcaatca gttaaaaaag ctttaagtcg tgcttattca tttattgcag
     2101 atactttatt tgagggacaa tttactggtg aagattattg caaaggaatt gatgctcgtg
     2161 agattgcctc attgacagga aaattattga agtctgttac tgcaggtttt gatgaaactt
     2221 atacagagac gaaaaaaaag taagtgaggc gctcaagtca ccttcattta agtattcgat
     2281 tacttaccga gaattagata taaagacgca attactcgaa gcaggttgga cgttaccaga
     2341 aattgaacgt actgacttgg atgagttaat gcgtcttttt gcttttagag atgcggtaaa
     2401 agagcatgaa gatgttgagt actatgataa tttcactcaa ttttaggagg tgatactttg
     2461 aataacgatg acctaattct gaagatgata ctagatgaat ctggttttac tgccggtatg
     2521 aataatgctg tcaaaaagtt gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat
     2581 agtggcagtt cattaggtag tatttggaaa atatttgctg gaagttttct agctagcgga
     2641 gtaactagaa tcgtaggagc tggctttgat ctaatcaagg gttccataag tggagcggtt
     2701 gatcgagtag atacgatgaa taacgctcta cgaaatttcc aaaatatggg atttagtaat
     2761 tcagaaatta tgaagaatat cggaaagaat gggcttttat ctcaaggtat tcaaggactt
     2821 cctactgctt tgaatgatgc gataagtcat gttcaacttc ttgcttcttc tacaggtgat
     2881 ttaactcgtt caactcaaat atttaaagct ttaaatgatg gaattcttgg tttcggtggt
     2941 tcaactgatc aagttaatga agctgttatt caattatctc aaagtttctc aaacggaaaa
     3001 gtagacgcac aaacttggaa ctcaatgatc aacgctcaac ttggacctac tctttctgct
     3061 attgctaaaa agatgggaat tacaatggga gatctgaaag aaggtttatc tcaaggtaag
     3121 atttctgttg aagaattcca aaatcaatta atagaaatgg ataccaaagg tggcggagga
     3181 cttaaatcat taagtcaaat cgctaaagat tctactaagg gaattaaaac ctctatacaa
     3241 aatgcaaaaa cagctgttac acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca
     3301 ttagtagatt cagatttagg tggatttaag ggaattattg ataaagtggg cagttcaatg
     3361 gaatcgttct taaaagtaat tgctgcaaat attcctacag cagtatcatt tttaagtaat
     3421 ttatttaacg aaattcaaaa atttggttct gctttgaaat tcatgatgcc ctttcttgtt
     3481 cctgcagcta ccgcttttgg agcgttcatg tttcaactta aaggtatacc agcaataata
     3541 aaaagcttca ataattttaa gaatgccata attggcgttg gaaattcatt aaagattatg
     3601 ggggcaatag ctgctgctaa tccatttgtt ttaattattg gtgcagtcgt tggtgcaata
     3661 gctgttttcg gttactttat ggcaaccaat gaagagctta gaaacaaggt tatatctatt
     3721 tggaatgatg tgaaagattc cgtaattggt gtattaaaaa atataaagga ctggggaatt
     3781 gatacttgga attctgctaa agaaatggca tcaaatgcag ttgagggtgt caaagatgct
     3841 tggtcaggaa caaaggaatg gttttctaac acctggaaag gtattaagga tggggccaca
     3901 ggtttatttg ataaaacagt agagacttca agaaatgcag tcgatagtgt aaaaaatgca
     3961 tggtctaata tgaagaaatg gttttctgat acttggcaaa gtataaaaga ttcagccaga
     4021 gaaaaatgga atggaatcaa aggttctatt atgtcagttg ctggtccatt gattacaggt
     4081 ataaagaatg catttttaca tgttactttt tatctagaga ccttatggaa taatttagtt
     4141 gagattggta aaaacgtctt tgagatttta aaaaatgtta tacttgcgcc ggttttgttt
//
LOCUS       DASJUF010000174         4045 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJUF010000174 DASJUF010000000
VERSION     DASJUF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.181"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(154..1659)
                     /locus_tag="U4B62_002782"
     CDS             complement(154..1659)
                     /locus_tag="U4B62_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2142880.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1656..2342)
                     /locus_tag="U4B62_002783"
     CDS             complement(1656..2342)
                     /locus_tag="U4B62_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2142881.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2535..3956)
                     /gene="gndA"
                     /locus_tag="U4B62_002784"
     CDS             complement(2535..3956)
                     /gene="gndA"
                     /locus_tag="U4B62_002784"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN2142882.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          856 c          691 g         1382 t
ORIGIN      
        1 tcatctaaaa gatacttaca tttaattaac ggatgttatt ctatttaatt gttcgctagt
       61 ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct tattctttgt
      121 tggatatata aaaaaacaag taaagcacat gctttacttg tcttctgcct ttgcgatagg
      181 aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga tacgtccttt
      241 atagttttca accagttgtt tagcgataga caaccccaga ccatttccac ccttcgttct
      301 agctcttgca ttatccactc gataaaaacg attgaagatc ttattaaggt cttctttggg
      361 tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg aagaaataga
      421 gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata tgataatgat
      481 taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca aatcatcatc
      541 taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt gataggtgac
      601 ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg ctcttgataa
      661 gtctagcatc tcttgaacta agcttttcat acggctgatt tcttgcatgc tggcgcttaa
      721 tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac taagatgccc
      781 ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga attgctcttg
      841 ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag ctaaatctgc
      901 caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat ctttacgtat
      961 agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag ataaaaagaa
     1021 acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag tcaataatag
     1081 cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat agccgatctt
     1141 ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt ttccttctag
     1201 tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt tttcatgggt
     1261 tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg gctgccctag
     1321 ctcagctata aaggaatcga tcttcattaa gtttccttcg accagtgaag tatgatctac
     1381 gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta ctaatgtcag
     1441 ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct ctacattgtt
     1501 tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag cgaaaattgt
     1561 aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca aggagggccc
     1621 tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac ataacctgtt
     1681 ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt gcgaagataa
     1741 cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca cactttattt
     1801 agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag cagcaactcg
     1861 tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg gttttctttt
     1921 tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt atcgccttca
     1981 atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat agcaaatggt
     2041 ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat cacagaatct
     2101 cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct gcagacttct
     2161 aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc attatttaat
     2221 gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc ttcatgcttc
     2281 aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag aatattgctc
     2341 atttattatt ttatcctttc tattctactg gctttttaaa aactctatca ttataccgat
     2401 aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa aagagctgaa
     2461 gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct catttcctaa
     2521 cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc ttctttgtcg
     2581 acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat atttgcaggc
     2641 agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt cggtacaggt
     2701 acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata ttttttcgtg
     2761 atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt gtcataggca
     2821 tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca gatttttgcg
     2881 atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa ttgagcaaat
     2941 ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt ttcgaccaat
     3001 tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt gcttgcttgt
     3061 acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt gatcaatgga
     3121 agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc tttattccca
     3181 gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc tttacgtgtc
     3241 agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt ccattcttta
     3301 aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa gtcataagat
     3361 tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt cacataatgt
     3421 ccggctccat ttgggccaat atatgtgaca catggtgctc cgtcttctgc ttttgcagaa
     3481 atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc aggcatgatt
     3541 gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat aaagttgatg
     3601 ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt atttcctccg
     3661 tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact cttggattgt tgcatctgtt
     3721 gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga ttctacaaat
     3781 tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac tacagcggtt
     3841 gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc tatgttcaag
     3901 gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt tgacatatga
     3961 actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa cacggctaca
     4021 ttgcaaacta aagaacaaca tattt
//
LOCUS       DASJUF010000175         3876 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJUF010000175 DASJUF010000000
VERSION     DASJUF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.182"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1029
                     /locus_tag="U4B62_002785"
     CDS             <1..1029
                     /locus_tag="U4B62_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2142883.1"
                     /translation="IQYDGLAKLKDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKR
                     NILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKS
                     HKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGS
                     AESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVV
                     LSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKV
                     IVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKP
                     KEILSKIS"
     gene            1039..1695
                     /locus_tag="U4B62_002786"
     CDS             1039..1695
                     /locus_tag="U4B62_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2142884.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            1695..3071
                     /locus_tag="U4B62_002787"
     CDS             1695..3071
                     /locus_tag="U4B62_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2142885.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3357..>3876)
                     /locus_tag="U4B62_002788"
     CDS             complement(3357..>3876)
                     /locus_tag="U4B62_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2142886.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1496 a          474 c          676 g         1230 t
ORIGIN      
        1 attcaatatg atggattagc aaaactaaaa gatgctaaag tagttagcgg agaacaaaga
       61 ataaatagag aagatttatc tgacgaattt aagaatgttg tttcactcga agctacaaat
      121 aatactaaaa gaaatatttt atttagtagt ggagtattta caattaaaga aggtaaaaat
      181 atagaagaaa atgataagaa ttcaattatt gttcatgaag aatttgctaa acaaaacaat
      241 ctaaaattag gtgatgaagt tgatcttgaa ttactagata ttgaaaaaag tggaaaaata
      301 aaaagtcata aatttaaaat tatagggatc ttttctggta aaaaacagga aacatataca
      361 ggattatctt ctgattttag tgaaaatatg gtgtttgtag attattcaac aagccaagag
      421 atattaaata attcagaaaa taatgaaatt gcaaataaaa ttttaatgta ttctggtagt
      481 gcagaatcta cagacttagc cttaaacaaa ttgaaagagc ttaaaattga tgagtcaaag
      541 tattttgttg aaaaagattc taatgcgttt gaagagtctt tagagtcagt gagtggaata
      601 aaatatataa taaaagtaat gacttattct attatgttag gtgggatggt tgttctttca
      661 ttaatcttga ttctatggtt aagggaaaga atttatgaaa taggtatatt cttatctatt
      721 ggaacatcta agatacaaat tataatgcaa tttatattcg agttaatatt catatcgata
      781 ccaagtataa tatcttcctt atttttagga aatgtattac taaaagtaat tgtagatgga
      841 ttcattaact cagaggattc aatgatttcc ggtggaagtt taataaataa tagtagtttt
      901 atgtcaaata taacaacact tggacaaagt tatttaatat taataagtat tattgtttta
      961 tcagttgtat ttgcttcttc attaatgtta atcaagaagc ctaaagaaat cttatcaaaa
     1021 ataagttagg agaaaataat ggatatatta gaaataaaga atgtagcata tagttatgca
     1081 aattctaaag aaaaagtttt gtcaggagta aatcaaaaat ttgaacttgg gaagttttat
     1141 gcgatagtag gaaagtcagg aacaggaaaa tctacacttc tttccttact tgctggactt
     1201 gataaacctc aaacaggaaa aatattgttt aagaatgaag atatacaaaa taaaggatat
     1261 agtaatcata gaaaaaataa tatatcttta gtatttcaaa actataattt aatagattat
     1321 ttatcaccaa ttgaaaatat tagattagta aataaatcag cagatgaaag tatcttgttt
     1381 gaactaggtt tagataaaaa acaaataaaa agaaatgtta tgaaattatc tggtggacag
     1441 caacaaagag tagctattgc tagagcattg gtatcagatg ctccaataat actagctgat
     1501 gagcctactg gtaatctaga cagtgttact gctggagaaa taattaatat attaaagaca
     1561 ttagctaaag atagaaataa atgtgtaata gttgtaacac atagtaagga agtagcagat
     1621 tctgcggata tcattttaga actaagtggt aaaaagttga aaaaagtaaa taaaatgaat
     1681 ttggaggttg aataatgata aaaaatgcat ttgcttatgt aactagaaaa agtttaaaat
     1741 cattaattat catattagtt attttatcta tgtcaacttt aagtctcatt agtttatcta
     1801 ttaaagatgc tacggacaga gcttcaaaag aaacatttgc taatataaca aatagttttt
     1861 ctatggagat aaatagacaa gtaaatccag gaacacctag aggtggggga aatgtaaaag
     1921 gtgaagatat taaaaagata tctcaaacag atagtataga ctcctatgta aaaagaataa
     1981 acagtgttgc agatttagtt gattatgata ttattgaaac tcaagagact cttgcgaatc
     2041 aatcacctga aagagcaaag aattttaaaa gaacagttat gttaactggg gttaacgact
     2101 caacaaaaga aacaaaattt gtatcagaag catataaatt agtagaagga aagcatttag
     2161 aaaatgaaga taaaaataaa atcttaatgc ataaagattt agctgaaaaa aataacctta
     2221 aagttggaga taagataaaa ataaaatcta atttatttga tgctgataat gagaaaggtg
     2281 cagatgaaac agtagaagta gaaattaaag gtctattcga tgggcataat agcggtgtag
     2341 taagtgcagc acaagaacta tacgaaaata cattaatcac tgatattcat acagctgcta
     2401 aagtttatgg taataccgaa gatacagctg tataccaaga tgcaacattc tttgtaaaag
     2461 gtgataagaa tcttgatagt gtaataaaag atcttgggaa attagatata aattggagag
     2521 aatataattt gattaaaagt tcatcaaatt accctgcatt acaacaatct atatcaggta
     2581 tttactcaat ttcaaataaa ttattttttg gctcattaat atttgcagga gttgtagttt
     2641 cattattatt attcttatgg atgaatgcta gaaagaaaga aatagcagta ttactatcat
     2701 taggtatatc aaaattagaa atttttggtc aattcctaat tgagatggta tttatatcaa
     2761 tccccgcatt cgttggttcc tatttcttag ctcaatatac agctgacaag cttgggaata
     2821 atatattgaa taaggttact ggtgatatag ctaaacaaat agctaaacaa tctgcatcta
     2881 gccaattagg tggtggggca gaagctgaag gatttaacaa gacattatca agtttagata
     2941 tcaatgtatt acctaaattc atgatttatg tagttatatt tatgagttta gtacttcttg
     3001 tatcattgat tatttcttca ttcaatatat taagaaggaa tccaaaagaa ttattaattg
     3061 acaataatta aaattttcgg tgctttttag caccaaaaat gagagtgtaa tataaaccgt
     3121 gtaagtaacc atcaatccta ggcgggtggt tgcttcacgg tttttatttt tctaaaataa
     3181 gatcaagagg tgattacacg atggctagaa agaaaagaaa tcctgatgct gagaagttag
     3241 ctgaatccat tttgaatact tatcaacccg aatctgtaga ggatatgcaa gatgttttaa
     3301 aagatgtgtt tggtccctta tttgaaaaaa tgcttcaagg agaattgaat aaccatttag
     3361 ggtatgacgc ccattctaaa gagccaaaga aacatgacaa tcgacgaaac ggttatggca
     3421 ataagacgct taaaacgagc tttggtaaag taaacattga tgttcccaga gaccgggagg
     3481 cttcttttga acctgagttg attcctaata gaaaacgaga tgtctccgac atcgcagact
     3541 gtagacaaaa gctgttccaa actcgtaact cgagcttgga acagcttttt atctacttcg
     3601 tttcgaattt ttgaacaaaa aaataaaggc tccttgcctt tccatagcca ggtggctaat
     3661 ttctttaaat tcattgcagc aaacactagc atcgtatgcg tcgcgacttt ttccaagcct
     3721 cgatacttgg tccaacgcat cccatgcttt tcttttgcat ccgcaaatat tcgttcaatc
     3781 gtttgtttcc gttttttgta aattgattta ttcagatctg ttaggcgaag atgttccact
     3841 tcatccatca gatctcccca aacatgtctc gtgatg
//
LOCUS       DASJUF010000176         3867 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.183, whole genome shotgun sequence.
ACCESSION   DASJUF010000176 DASJUF010000000
VERSION     DASJUF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.183"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(382..1449)
                     /locus_tag="U4B62_002789"
     CDS             complement(382..1449)
                     /locus_tag="U4B62_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2142887.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1469..1948)
                     /locus_tag="U4B62_002790"
     CDS             complement(1469..1948)
                     /locus_tag="U4B62_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HEN2142888.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1965..2426)
                     /locus_tag="U4B62_002791"
     CDS             complement(1965..2426)
                     /locus_tag="U4B62_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2142889.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2440..3690)
                     /locus_tag="U4B62_002792"
     CDS             complement(2440..3690)
                     /locus_tag="U4B62_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEN2142890.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT         1201 a          806 c          505 g         1355 t
ORIGIN      
        1 cttggaataa tgaaaatata cttttgtatt acacaatatt gcaattttat ttcttaatat
       61 gattatttca ctcattcatt actgccacat atacacaact tataaccacc ccctgcaaag
      121 tttagggtaa gttatcagat aatatcttcc tcttcatcat ccagaataca aaatctattt
      181 atttatagaa atatcattaa tgtttttata taatgtaccg tgcttgtact cttgctctat
      241 tacaagcact aaaacactac tattcatttg aattcttaca cggataaaaa tttacatcgt
      301 ctatttcaat attttttctc tctattttat tcacttaata agttcattac ataatcgaaa
      361 cattttttat tttccttgtc atcactcctt gctccctttt tgatacagat ttaaaattct
      421 atcaaagaaa tttccatcgt taaatttcgt tattgccaaa tccttcgaac aacgtgaaaa
      481 ttttacgtat ttatctttat cattgatttt tagtattgca gatgtcaacg ctgatacgtc
      541 accaggttga aacaaatatc cattttctcc atcacgtact gctgaagaaa tatctcctac
      601 atctgtagca attacaggca ttccataaga aatcgcctct agaatagata taggcaatcc
      661 ctcgttatag gacggtaaaa tcatcacatt tgatcttcta tagcactcct cctttttttc
      721 tcccacaacc caacctgtaa attcaaaata cgattgcaaa ttatattctt ctatcaattc
      781 tttgagcgat atttcttcat cacctgctcc agcaatctca aaaataaact ttttttctgg
      841 atattgttct gcaatgtttt taactgcttc taccaaatcg tgtacacctt ttcgctttat
      901 caaaactccc ataaacagaa atcggcacat ttttgaattc cactttatgg tatattctgg
      961 aactacaata gaattttcta caacaacagt tttacaattt ggctcaattt ctttaattat
     1021 cttcttccat ttttctccaa gtacaatcgt ggaatcagac tctcttaaca gcaagcgaat
     1081 ctgattttgt ttttttacag aaacactatc ataccatttt ttaaattccg atccatgaag
     1141 atggatgatt acaggaatat ttaataatct aaaccactta ttcagcacat atttcctact
     1201 aaaactccct ctgtacgaca tgtgcatatg aataatatta ggtttactca ataaagagat
     1261 acctataatt ttagcaaatg catacataaa atataaagat ttaaatattg catttccacc
     1321 cttataactc ggtataaaag tcatctcgat attctttttt tcccaattaa aattgagcat
     1381 ttgtgtaatt atacttgtaa ttccaccttt taccgagctg tcattaccaa tcataagtac
     1441 tctttgcata acgctttact ccttatattt atattttttc tattaatacc tctatcgaat
     1501 taataatcgt ttccgtgtta gatacatact tgttaaacat ccgaactctt gtttctttaa
     1561 cggcatcttc taaattttct aattcataac aaggatagat aatattcatc tcatcaaatt
     1621 gtctaattaa ctgcagttgg tgatcatcga catgctcacc atacttagcc aatcgaggaa
     1681 cagcaatgac ccttttacct ttttttacgg ccccaattat agcacctgta cctccatgag
     1741 taatcaccat ttctgctttt tgcatagttt cagtaaactc atccctattt agaaatgctt
     1801 tataggaata atttagtggt ttataatcac tataaccact ctgtgcaaat gtactttcga
     1861 ttattactcc tttttcaatt aaatcatcaa tagcttttaa caatctatca aattgaaatt
     1921 tttgtgaacc taatgttata aatatcattt ttttctccta ttatttaata tatcccaccc
     1981 aaataaatag cattaggata aaattgtagc atagtttccc actgaacata aaattgatct
     2041 gcaaaatgat acatcaattt tcctgtctga gtagggctgg ttacttttgc aaacgattca
     2101 atataaatca gtttttttcc catcattttt gttattaagc agatagggat catagctaaa
     2161 acacctgtac aaatcacgag atccggtttt tcctttatat atataataat tgatttgaat
     2221 aaattaataa gtatagtaca caaaaaaaac ttttcttttc tatttacttg tctcatgtag
     2281 tacgtttttt cgccttttga ctcagctgaa tattctgtct gttctgttat aacaaaactc
     2341 tcatacttct ccatcagtgg ttttaacatc attaactgtt cataatgccc cccagaggaa
     2401 gccgcaaaac atatcttttt gttcatatta acctctctat cagtcacgct gaaaaatatc
     2461 tctagtgtaa actttttctt taatatcatc caagcaggta tcatatcgat tggcaataat
     2521 agaatcacac atcgccttaa actgctctaa atcgttgata accttacttc cgaaaaaagt
     2581 ctctccatct ttcagtgcag gctcataaat caccacagtc gcacctttag cttttacgcg
     2641 cttcataact ccctggattg aactctgacg aaaattatca ctgttgctct tcatggtcag
     2701 ccggtatata ccgattaaaa ctggattttc cctgttgaca tcatactgat tatattcagc
     2761 gtagttgtaa tagcccgcca tctgaagaac acgatctgcg atgaaatcct tacgggtacg
     2821 attagattca acgatagcct caatcaaatt tccaggaaca tctgcatagt ttgccaacaa
     2881 ctgcttggta tcttttggca agcaataacc tccatatcca aaggatggat tgttgtagtg
     2941 agttccaata cgaggatcga gacaaacacc attgattatc tgctgagtat tcagcccctt
     3001 catttcagca taagtgtcta attcattgaa atagctgact cgcagtgcta aataggtatt
     3061 tgcaaacagc tttactgctt cggcttcagt gaatcccata aaaagagtat ccacgttctc
     3121 cttaattgca cttttctgca gaagtgttgc aaaaacatga gcagcattta acaagcgctc
     3181 atcagtcata tcagtaccaa caataatacg agatggatac agattatcat ataaagcctt
     3241 gctctcgcgc agaaactctg gactaaaaat aatgttctta ctccccgttg tctcacggac
     3301 tgattcagtg tagccaacag gaattgtaga cttgatgacc atgatggtat caggattcac
     3361 gtccatcaca acctcaataa ctgactcaac agcagaagta tcgaaatgct gagtacgact
     3421 gtcatagtta gtcggtgtag caataacaac gaaatctgcg gatttataag cgctccttgc
     3481 atccaaagta gcagttaagt caagttcttt ttctgtaaga tacttttcaa tatattcatc
     3541 ctgaattgga gacttccgtt tattaatcag ttcaatcttt tcagggataa tgtctaccgc
     3601 ggttacctgg tgatgctgcg ccaatagtgt ggcgatactc aaaccaacat aaccggtgcc
     3661 ggctactgcg atgttgtact ttttcttcac aatatctcct ctttcctctc ttaagtatgg
     3721 agaatgttac tcctacggct attcatcatg tcaaaataac acagcttaga atgttgataa
     3781 aataattttc cagaatgtga taaaatctct ttcatttata aaccacacat ctaattttat
     3841 ctattctcca caagacatgt agagtat
//
LOCUS       DASJUF010000177         3863 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJUF010000177 DASJUF010000000
VERSION     DASJUF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.184"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(245..466)
                     /locus_tag="U4B62_002793"
     CDS             complement(245..466)
                     /locus_tag="U4B62_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142891.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="U4B62_002794"
     CDS             complement(564..809)
                     /locus_tag="U4B62_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142892.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="U4B62_002795"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="U4B62_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="U4B62_002796"
     CDS             complement(2033..3034)
                     /locus_tag="U4B62_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN2142893.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="U4B62_002797"
     CDS             complement(3193..3459)
                     /locus_tag="U4B62_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142894.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="U4B62_002798"
     CDS             complement(3449..3805)
                     /locus_tag="U4B62_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2142895.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DASJUF010000178         3841 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJUF010000178 DASJUF010000000
VERSION     DASJUF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.185"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..332
                     /locus_tag="U4B62_002799"
     CDS             <1..332
                     /locus_tag="U4B62_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142896.1"
                     /translation="LLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSIS
                     LCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNF
                     YFYRNKL"
     gene            complement(458..1135)
                     /gene="nth"
                     /locus_tag="U4B62_002800"
     CDS             complement(458..1135)
                     /gene="nth"
                     /locus_tag="U4B62_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN2142897.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1142..1840)
                     /locus_tag="U4B62_002801"
     CDS             complement(1142..1840)
                     /locus_tag="U4B62_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2142898.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2108..3292
                     /locus_tag="U4B62_002802"
     CDS             2108..3292
                     /locus_tag="U4B62_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN2142899.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3298..3741)
                     /locus_tag="U4B62_002803"
     CDS             complement(3298..3741)
                     /locus_tag="U4B62_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2142900.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1001 a          759 c          635 g         1446 t
ORIGIN      
        1 tacttttaag cactactctt aaaggaaaat ttatggaaag aagctctcat gcaatgattc
       61 tcactttttt atggttgttg ttcaccagaa tctatatttt tctgatgagc ctgaaaccgc
      121 ccaaatccat tagtttatgt ttagttataa atggtatttt tttattatca cttatcattt
      181 ttattacacc tttagataga tacgtcactt atttatataa tcctttggaa tactggactt
      241 attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata
      301 acaactttta tttttatcgc aataaattgt aataagttac catcctagct actgagtatg
      361 tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga
      421 taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta
      481 gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac
      541 atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat
      601 gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca
      661 tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat
      721 ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc
      781 ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt
      841 caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg
      901 tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa
      961 aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta
     1021 agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg
     1081 gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc
     1141 gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact
     1201 gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt
     1261 gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac
     1321 tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat
     1381 ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa
     1441 attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt
     1501 attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat
     1561 atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta
     1621 cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca
     1681 tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata
     1741 aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga
     1801 ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc
     1861 acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact
     1921 gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt
     1981 atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca
     2041 aggaaacaaa attactatac actatttttg tttccaagtt aacattattg cataaaggag
     2101 tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt
     2161 attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta
     2221 ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct
     2281 agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc
     2341 gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa
     2401 ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc
     2461 caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg
     2521 atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt
     2581 agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc
     2641 cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt
     2701 aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt
     2761 tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat
     2821 cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt
     2881 ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa
     2941 aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct
     3001 attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg
     3061 ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt
     3121 ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg
     3181 cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt
     3241 attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca
     3301 tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc
     3361 ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg
     3421 ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt
     3481 attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt
     3541 actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg
     3601 gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt
     3661 ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat attcttctat
     3721 tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg
     3781 tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa accaagaaaa
     3841 a
//
LOCUS       DASJUF010000179         3820 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJUF010000179 DASJUF010000000
VERSION     DASJUF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.186"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U4B62_002804"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U4B62_002804"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN2142901.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="U4B62_002805"
     CDS             complement(2260..2511)
                     /locus_tag="U4B62_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN2142902.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="U4B62_002806"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="U4B62_002806"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN2142903.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="U4B62_002807"
     CDS             3461..3679
                     /locus_tag="U4B62_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142904.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1059 a          812 c          711 g         1238 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt
//
LOCUS       DASJUF010000180         3696 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJUF010000180 DASJUF010000000
VERSION     DASJUF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.187"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2790)
                     /gene="brxC"
                     /locus_tag="U4B62_002808"
     CDS             complement(<1..2790)
                     /gene="brxC"
                     /locus_tag="U4B62_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN2142905.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQ"
     gene            complement(2815..3390)
                     /locus_tag="U4B62_002809"
     CDS             complement(2815..3390)
                     /locus_tag="U4B62_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HEN2142906.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3390..>3696)
                     /locus_tag="U4B62_002810"
     CDS             complement(3390..>3696)
                     /locus_tag="U4B62_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HEN2142907.1"
                     /translation="NEFMTEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDY
                     TIKRLIAAYQTNLIETGLVRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1096 a          714 c          509 g         1377 t
ORIGIN      
        1 ttgtaaatca aaataagagc gtaaattgtc taattgacct tttaaatatt tcttgaaatt
       61 ttccattgta tgttcattgt tattttcatc aacaatatta cgactttgaa acagttctct
      121 accgatttcg tcaaccaatt tttttgcttt tggcgaaatc accgcgcttg gttcaatttt
      181 tatcttttca ctttctcgtc gtttttgaat aatcattaca atactatttg gatcatccat
      241 ccgtgaaatt tgtacattgt tataaagtaa atcaatttta ccagacatga aaagtttagc
      301 gatgcaccat tccacatcaa gttctgtgaa tccatacggt tgttttgttt caaaacgatc
      361 acgaattgtt ttcatcgtaa tcgttaggtg atttctcttt tgatcattga taaaattgaa
      421 aatttcctga actgcttgct catttgatgg atcttcaata atttcatttt tgctttcttt
      481 cactaaatat aagatatctt ctttttcttt aggcgcatca atttggttta atttatggta
      541 tacttcttca gccattttct gaagtgctga ttggaatttt gctttaaagt cggagcctgt
      601 cagttgaatt tctgcaccgt tatagaaaaa ttttgcagat ttcaacagtt catttaattg
      661 atcgttgata cttgcactta gttcctgtaa ttcaccagat ttttccgaac gaatcgcgtc
      721 aaagttcttt actacactcg aataagtcgt tgttaaataa agattcattc tagcatacat
      781 tgctatatca tctttaaatt ctttatctga tggcaattct acgaataata agttacggga
      841 actcttaagc gcaagttctg cctcttgacc tgctaaatca gagtatggtg aaataaaatt
      901 aactgtcatt tcagcatttc cagcttgctt gtatggacgt tgatctaaca cttgtgcata
      961 agtaaacgta taccggtttt taaatttatt tccacctgac atgcctaatt tttcataggc
     1021 aaaactattt cggtctagaa cgccgtcaaa aattcgttta gatagttcta cgatcaaatc
     1081 actatctgta atattttgat ttttaatctc acgagtaatt tcctgttctt catcagtttg
     1141 gaaaatataa ctatctaagt tcttccgcac aaagttttcc ttaattaaaa ggtccaatgc
     1201 ttcttctact cgttttttag tttccaggcg gtcttcgttg atatttctta tcatcaagct
     1261 cgtaatattt tcaacatttg gtttgatggc ttgatcaaca tatttaatca tgaataaaat
     1321 tcgtaagaca tttacgttaa agttatctat atcagttggt ctttgaatat gagaattatt
     1381 tgtagcttgt gtaatcactc ggctaattgc tgtatcaatc caacgttcta acgagtaata
     1441 aaactgatct aaagaaacca aactggttgt atcattttcc ttatttcgtt cagcggcttc
     1501 tttaaacatt gctagtaatg aacgttcacc agacgataag tttgatcctt gatagctgtg
     1561 attacgaatc tcggttagaa tttgccccaa taaattgaat tgatagggaa taaatggata
     1621 aacttctgcg taattttcag catcttggaa gagctttaat tcaggactat caacaaagag
     1681 taatttattt ttcaattctg tttgaacagt ttcaaattgt aaggctaaag aatcattggc
     1741 aaactctttt ttcttcaaaa tacgttcttt aattacttca tcggcatttg ctgaagataa
     1801 actcacagtt gtggcaaaac gatccttgat cttagaaaaa tcattatcag aattgttatc
     1861 aaagtttgtt acgtgatcaa tcgcttcctg acttgtcact aatacccaag ccttaccttc
     1921 acaaattcga cccaaatctt ccacaacggt ttgtaactgc aaagtcaact cacgattacc
     1981 gccgatatat tgtccgacct catcgactag aaaggcaata tgatggtctg cttctttgga
     2041 atcaagatag cttttaacta gatttgcaaa gtcatcaatg cttattaaat aatttcctga
     2101 tgctttttct aaaaaattat caatagcggc ttcactcata aaatcgattt taagtgccgc
     2161 ttctttgata tcatcttgga tccaatcaaa ctcatggcgc tcttcttgcc attttgcccc
     2221 attaatctct tcaaaagcag ctttgaatgc atcaaacttt tcttctttaa ttaaccgacg
     2281 ttcaaaatct gctagatgtg gaatcgtgct taatccttgc atttcgttaa atacctttaa
     2341 aaaaacattc acgattccgt ttttgtcttt tttaccacct gcatcacttt ttgaatcaat
     2401 attaaacaga ataacatctg tatttatttg tgagcaccgt tccatcgttg ccaaagtcag
     2461 tggatctttg attttcccat cttctttgaa gtaatcaatg gctttcttgt catttatttc
     2521 tcgatttgcg agcaagtaag aaataatttt taagaaatga gatttacctg aaccaaagaa
     2581 cccctgaatc caaacaccca ttttatctgt tggtgtattg atactccgtt ggtaggcatc
     2641 aaaaattgtt ttaaagtgtt tttgtagttc tttcgtaacc acgtattctt ctaattcttg
     2701 ttgaacattt tcatcctttg cttgaccgac cttaataact ccttgaatat ttcggtcaat
     2761 tggttttgca aaaatatctt taattagcat ttaatctcct accttccatc ccaactaaat
     2821 tctaaatgct cgataataat tatcatcttt attggtatta agaattgtca aacttaattc
     2881 atcatattcc cctggataca tcatcacaac tggtacgcga tcaaatacct gatgcatttt
     2941 atttaaaata gagtgcgctc taattaatgg atataattcg ccaattcctg tcaggaacac
     3001 aactgatttt tctggtgtat gggcatccac atattcaaaa aatacatttt cttcctcagg
     3061 taaatccatt ttcaataaac gaccaatgct ttccactaat ttttgtaaac cacgtcgttc
     3121 ttccatctta atcgcttttt cagtgaaacc gcgtttttct agatactcta acataatcac
     3181 atagagatta taaactttca cttcataatt atcaccaaca gatgttccag atatatcatt
     3241 aatataatct cttacaatta actcgtcttt cggatcgtag tcaaagatat aatagcctac
     3301 ttcattacct aaaccacgat tcaataaaaa atcttctttt tgaattactt cattcaactt
     3361 ttccaaatgc tcttgaatcg ataatgacat tataaaattc ctcccagtgc tagtacaata
     3421 tctttacgat tcttgtcaac taaccattga atcaatcgtt tatctaataa tatcggagtc
     3481 attcgtccat attctttttc ccctctgact aatcctgttt caattagatt cgtttgataa
     3541 gcggcaataa gccgtttaat cgtataatct ttccattttg aaatatcttc tgaacttctc
     3601 cgcttctctg taaaaaaaga acggtaatca actggtgtta attgcaaatt tcctttacgt
     3661 agattttcag catatacctc agtcataaat tcatta
//
LOCUS       DASJUF010000181         3664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJUF010000181 DASJUF010000000
VERSION     DASJUF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.188"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..277
                     /locus_tag="U4B62_002811"
     CDS             <1..277
                     /locus_tag="U4B62_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEN2142908.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="U4B62_002812"
     CDS             420..641
                     /locus_tag="U4B62_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142909.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="U4B62_002813"
     CDS             657..1499
                     /locus_tag="U4B62_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142910.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="U4B62_002814"
     CDS             1562..3364
                     /locus_tag="U4B62_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEN2142911.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="U4B62_002815"
     CDS             complement(3423..>3664)
                     /locus_tag="U4B62_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN2142912.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DASJUF010000182         3651 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.189, whole genome shotgun sequence.
ACCESSION   DASJUF010000182 DASJUF010000000
VERSION     DASJUF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.189"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            29..781
                     /locus_tag="U4B62_002816"
     CDS             29..781
                     /locus_tag="U4B62_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2142913.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            778..1692
                     /gene="pfkB"
                     /locus_tag="U4B62_002817"
     CDS             778..1692
                     /gene="pfkB"
                     /locus_tag="U4B62_002817"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN2142914.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1710..3641
                     /locus_tag="U4B62_002818"
     CDS             1710..3641
                     /locus_tag="U4B62_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2142915.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1174 a          658 c          835 g          984 t
ORIGIN      
        1 tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgcagac aaaaaatcct
       61 agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc ttttgaatgc
      121 ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag
      181 agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa
      241 atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa
      301 tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct
      361 gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga
      421 tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt
      481 aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat
      541 gaacggtgcc catcttgaac aaggattcac gactccggat ccagaagagg cagctgttaa
      601 acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa
      661 ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg
      721 tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg
      781 atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa
      841 ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat
      901 gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg
      961 ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca
     1021 accgtaaaag aagatacaag aatcaatatc aaactaaaat cagacactga aactgagatc
     1081 aatggcttag gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga
     1141 acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccagctag cctgcgaaaa
     1201 ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat
     1261 acaacaggtg atgacctaaa aaatgcactt gttaagaaac cgttgttgat caaaccaaac
     1321 aatcacgaat tagctgagct ttacaacaca accttccaat ctgtagaaga aatcattccg
     1381 tatgggaaaa aattactagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac
     1441 ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg
     1501 aaaaattcag taggtgcagg ggattcaatg attgctggct ttattggggc ttacagccgt
     1561 acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt
     1621 tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca
     1681 aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag
     1741 cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa
     1801 aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc
     1861 gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca
     1921 gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag
     1981 ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg
     2041 acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag
     2101 gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag
     2161 agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc
     2221 atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt
     2281 acatggcaga agatgctttg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg
     2341 aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag
     2401 aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac
     2461 tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagttgatc aatctagccc
     2521 ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa
     2581 atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca
     2641 tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat
     2701 ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct
     2761 ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta
     2821 ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag
     2881 ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg
     2941 tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa
     3001 cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca
     3061 atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt
     3121 cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc
     3181 caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg
     3241 gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt
     3301 tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga
     3361 cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg
     3421 acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg
     3481 gagctttcgg aatcaaatta ctggctcctc acggcgggat tttcgttgta ttcttactga
     3541 gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt
     3601 atggtttatt gagaaaagct cctgcagtag cagaagcata gaaataaaaa a
//
LOCUS       DASJUF010000183         3602 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.190, whole genome shotgun sequence.
ACCESSION   DASJUF010000183 DASJUF010000000
VERSION     DASJUF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.190"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..186
                     /locus_tag="U4B62_002819"
     CDS             <1..186
                     /locus_tag="U4B62_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2142916.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="U4B62_002820"
     CDS             329..553
                     /locus_tag="U4B62_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142917.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="U4B62_002821"
     CDS             568..1437
                     /locus_tag="U4B62_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN2142918.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="U4B62_002822"
     CDS             1418..2152
                     /locus_tag="U4B62_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2142919.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="U4B62_002823"
     CDS             2185..3093
                     /locus_tag="U4B62_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN2142920.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="U4B62_002824"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="U4B62_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DASJUF010000184         3580 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJUF010000184 DASJUF010000000
VERSION     DASJUF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.191"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..186
                     /locus_tag="U4B62_002825"
     CDS             <1..186
                     /locus_tag="U4B62_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142921.1"
                     /translation="ISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGV
                     SPKQYQLGLVDNFNNIS"
     gene            203..637
                     /locus_tag="U4B62_002826"
     CDS             203..637
                     /locus_tag="U4B62_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142922.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1254..>3580
                     /gene="esp"
                     /locus_tag="U4B62_002827"
     CDS             1254..>3580
                     /gene="esp"
                     /locus_tag="U4B62_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN2142923.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1305 a          512 c          688 g         1075 t
ORIGIN      
        1 atctctaagc gaaaaatttc tacggctaaa ataatgttga aatcaaacca tactatcagt
       61 gaagtttctt taggtttagg attttcagat tcttcacact tttcgagagt attcaaaaag
      121 tacgcaggtg tatctcctaa gcaatatcaa ttaggtttag tggacaattt caacaacatt
      181 agctgagagg ttttatttta ttatgagact atatcattta cgtagtttgg aggagttgag
      241 gaaattggat tttaacaaaa aatttttttg ggaaatttct gatttgagtt gtttcgtaga
      301 atctgagcgt ctaagtaatg gtaataatag tgtgttaaat gaatatgaca aagtagtaat
      361 taataaagta aaaagtttat gtgaagaagc tagagaatct atcagtcata ttaatggtag
      421 caattttaaa caaacattat ttaatctgat taagctagat gctaaaatat caagttattt
      481 attctttttg atacatgatg aatttatgga ttatcaaaaa ttggatcagt taattgaaag
      541 agaaagctgg gaagattatt acgtgggtct aactttttca gaaaagttaa acaactacaa
      601 gttaatagat tataagtatt tgagtgaaag caattaatta ttcatgtaga gtactagaca
      661 ctttaaatat ggacaattca attataaaga aaaagaatca ataatttaat gaggctgtga
      721 caaatgtcat tttttgtttc gctaaaaacg agtgaaaaaa tgttgataac tccaatcatt
      781 cgtttaagat tctaggaatc attcagaaat gctgtgaaac cgatacttta taattataaa
      841 gaaggtgatt tcaaattccg aagaaggaat ttgaagtcac ctttttactt atgtcacagt
      901 ctcattcagt aatgcgggat aaggctcctt attccgttat ttgaatactc tgaagtttaa
      961 tctaagaaag ggtaatattt tgaaaacatg cttcatcgca tagaaatgaa cttaatttca
     1021 gaaagaatac atacatatct ttttatatgt tttcagctgc tgaatggtta aaaagcacaa
     1081 atatccgaaa aaaacatgaa gaaatgtact ttttttgttg taatgttcat gaaattctct
     1141 aagtaaatta gttaacataa ggttagtaag attcctattc tgtgaataat acgtatcaga
     1201 tttatctttt attcttacta ggaaagtaat tattaaggga gttgatttga ataatggtta
     1261 gcaagaataa taagagagta tttctagaaa aaacaaagaa acgagtattg aagtacagta
     1321 tcaaaaaatt aagtgttggg gttgcatctg ttttgattgg tgttggtctt gtcttgggaa
     1381 caactgaact agttaaagca caagatgaaa tttctccaag cagcccactt gaaacagcga
     1441 taagctccgt acaagtaggt gacaaagtag cttcggggaa tacttttcag gagaatcctg
     1501 gttatacgaa aaattataac ttttcggatt tacaatttag tcctcaagaa ctaactggag
     1561 acacactaaa aggcaataca attggatttg aagtttacgg aaaacataat attgcagcat
     1621 caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg attagctaaa tatgtagaaa
     1681 aaattcaggt cgatccaaag aaaggaattg gatcttctag acggaccttt gtaagaatta
     1741 atgattcgct gggtagacct acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg
     1801 gattatttgc tggggcagaa acaaccgata cgcaaaccgc tcctaatggg gtaattacgt
     1861 ttgaaaagag cctagatgaa atttttaaag agataggcat agacaatctc aagaccgatc
     1921 gtttaatgta tcgaatctac ttggtaagtc atcaagacga tgataaaatt gtaccgggta
     1981 ttgatagtac gggctacttt ttaacagatt cagatgattt ctataatagc ttagatgtat
     2041 ctgaaaataa tcctgaccag tttaaacatg gatcagtaag tgcaaaatat gagaaaccta
     2101 atactcaaac aaaagatggt tcaggatcca caggtgctaa tggagctata atcttagatc
     2161 ataagttaac gaaaaattat aatttctcat attcatcttc cgcaaagggc acgccttggt
     2221 atgctaatta caaaattgat gaacgattag ttccatatgt agcgggtata cagatgcaca
     2281 tggttcaggc tgacaaagta acatatgatg tttcgtttga atccggaaca aaagtagctg
     2341 atttagcaat cgagcgacgg gaaggtcatg aaaattatgg cataggttca ataactaata
     2401 atgatttaac taaacttatc gattttgcta atgcaagtcc acgtccagtc gttattcgat
     2461 atgttttaca attgactaag ccattagatg aaatcttaga agatatgaaa gcaacagcac
     2521 aagttgaaga aaataaacca tttggtgaag atttcatctt tgattcttgg ttgtcggata
     2581 cgaataaaaa attaatccag aacacttatg gaacaggtta ttattatttg caagatattg
     2641 atggtgatgg aaaccctgac gataaagaag agagcggaga cacgaatcca tatatcggga
     2701 aacctgaatt agaagaagta tatgatgttg acacaacagt taaggggaaa gtattcatcc
     2761 acgagttagc gggaacaggt cacaaagccc aacttgttga taaagaaggt actgtattag
     2821 cagaaaaaac tatcgctcca aatgaaaaag atggggctcc aatttcagat actgtagaat
     2881 ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa
     2941 tcgtttctcc aggttttgat aaaccagaag aaggttcaac cgttattaag gaatcaccaa
     3001 aagcggttga taaacaaacc gtggtagttg gatttaaacc agatgctaaa gaatcaattc
     3061 ggaataataa aaacttacct gaagatgcag agtattcatg gaaaacagag cctgatactt
     3121 ctaacgttac tgatagtacg aaaggtattg taactgttaa gatcggaaat cgaactttcg
     3181 acgtggatgt agagtttgct gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg
     3241 tacctataac aacaactcca gaaacgacag ttcaaagtgg aaaacctaca tttgataaac
     3301 cagatgttcc tttagctaaa gatgcctttt caatttcaga tgtctataat aagtacttcg
     3361 gtaatgcaag tgttgatgca aacactggta ttgttacatt cactccagct aaaggtgtag
     3421 gagaatcgga gccgattact ggaacaattc ctattaaaat tgtttaccaa gatggttctg
     3481 taggcacgac cgatttagca gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa
     3541 acattcctgc aggctaccac aaagtaacct tcaccgcagg
//
LOCUS       DASJUF010000185         3429 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.192, whole genome shotgun sequence.
ACCESSION   DASJUF010000185 DASJUF010000000
VERSION     DASJUF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.192"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            252..464
                     /locus_tag="U4B62_002828"
     CDS             252..464
                     /locus_tag="U4B62_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142924.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(742..2541)
                     /locus_tag="U4B62_002829"
     CDS             complement(742..2541)
                     /locus_tag="U4B62_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2142925.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2665..3261)
                     /locus_tag="U4B62_002830"
     CDS             complement(2665..3261)
                     /locus_tag="U4B62_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2142926.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1259 a          617 c          597 g          956 t
ORIGIN      
        1 taaatcatat gctcacctct ttataaataa cttagcaata taaagaaaaa aacgcacgaa
       61 caacatagga atataaacaa acgtttttgt cacaacgtct aagatcgtgt taaaaaatag
      121 ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa aaaatggata aaaacgtttt
      181 caaatacaag gtaatttgtt attataaaat aaagaaaaaa caaaataaaa ataaagaaaa
      241 gagggaatga aatggtaaaa gcagaagtag gaagatctta tatatgcaaa ccgatcggag
      301 tatctcatga actaatcggc tgtattgaac gtacatatat caacacggct gtcgtgtatg
      361 ttgaaagtta tgaccaacga gactatcaac ttatcaatga aagcaagtat cgcatgctcg
      421 taaaattctg tgatattagc gcacctgctg aattggtttc ttaaacagta tttttgagaa
      481 ttcgcaagaa ctcccttata atataaataa agacagaagg gagcgagaag atatgccaaa
      541 aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg atctctgcaa ttcctgctat
      601 tttagctatt gttcgtgcga tgaaggaatc aagagaaagt taagcacaca ttatgagcgt
      661 caaacaaaaa acgcatatac ctgcggtggc aggttatgcg ttttttgttg tataaagact
      721 tttttcatta acgatttttg attagccgac tcccaaaaca aaatttagca gtttatctga
      781 ataagtgatt tcttcatctg tttgtttcac actttcattt agtagtccca tctcatcggt
      841 tatgatacca tccacaccat aaaacatcat gcgactcata gcatctgaat cgttgattgt
      901 ccatacatat acttcttttc cgtcttggtg ggcagaatta atgaagttac ggttgatcgt
      961 actatattcc attgtcagaa aatcagcagg cgtcacaggg ggcccaacaa tattgaaagg
     1021 taagatgtag cccacataaa agtttggttc tgcttctttt aaatctgaaa cggtttggta
     1081 cgtcaaactt tgcaaaatat gatggttctc taagataact ttgcggtatt tatggacaaa
     1141 tcgttcgaca aggtcatccg aatctttttt agaggtcttg atctcaataa gtaatttttg
     1201 gtttagctta tgtgcttttt ccaaataaga atcaaaagaa caaacagggg cttgcactcc
     1261 gttttcagta actgtaagat ttgtcaattc tttcaatgta agatcctgtg gtgttccatt
     1321 tacccctgtt aagtgacgaa gatttgcatc atgcatcact acgaaatgac catcttttgt
     1381 ttcctgaaca tccatctcta cataatcagg atgatagtct ctgcttgttt tttcaagtgc
     1441 ttctaaagta ttttgcgccc cattttgggt actcacacct ctgtgagaga cagtcactgg
     1501 ttttgtctga acagctgaag tcagataatc catattgtaa agacagacgc caataccgaa
     1561 aaaaactgca aaaagagtca aaccagtgtt tttcagcgca gagaaacgca agttgggggc
     1621 ttgtgggata aaccacttag ggatctcagg caagaagcct tcgtcatcca tgaaatcaac
     1681 aatgatataa aaaatcccta cggtagacat cacgatattg aataataaaa tcccttgtaa
     1741 aagcgtcata gcaaacacag cgctgatcaa tgcatagtct gggaaaaatt gttcaaccat
     1801 actttgtgcc aaaataacaa cgatatatcc taaagaagaa aggagtaaaa tcgtaccgcc
     1861 aatcacaata aattggcccg tgatggccaa aagacgcgat ttggtcaatg accagctttc
     1921 ccgaatggct gctcgaaaag gacgatctcg tagaatcatt tccggtaaag caaaaatcag
     1981 tcgaatccca atatagccaa ggaaaatata aacgagtaaa aaggaactga cgatgatcca
     2041 acgattagca aaaataaaat ccataatgaa agcggggatt tttatttttg acagtagatc
     2101 agaattaaaa cttaatccgc tgatcggtag aatcaataaa aaataagcaa ggaaaaacaa
     2161 aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc aagtgtaaga gttgcttcaa
     2221 agagattggt tctttctttt taataaaaaa aacactcatc aaaagaaaag taaattcgaa
     2281 ataaactaac agtaaaatcg tcatcaaaac tacgattaac gagagtagaa ccccaggatg
     2341 ttgagaaaaa attgaaggaa tattgtcata agacaaatag tcaattgccc ctcggtgtaa
     2401 aataaaacgg gtcgtactgc tcaataaggg taaacaaata aataaaataa agccatgcat
     2461 cagcaagaca tcacggaaat aagcttgtgt tccttttaaa aagtcccaag cattacctaa
     2521 gctacgtttc aaataattca tcctatcacc tgctcttcac attgtagcgc aaatctaatg
     2581 aaaaaggaaa gggtgcagtg tgttttgcag accctttccc agatttaaca ttgtcaatca
     2641 atgactaatt ttacaaaata aatattatcc aagaagacca cttagatatt gacctccata
     2701 aatcaaggcc atactatata agaaaatcga cagtggttta gcaatcaaaa tgattagtgt
     2761 atatcgtttc actgacatgt ttgtcaatcc agccatcaaa cataacgcat cgtcaggagc
     2821 aactggaaga aaaatagcta gagcaaacag cttctcaaaa cggttttgat tatccagcca
     2881 gccaatatat ttgttatatg tcttatcgct aatcatggaa agtatgaatg gctttccata
     2941 acgacgccct agcagaaaaa tgataatcga accaatgcag attccaatat agttatagat
     3001 aaatccagca taagggccaa atatcaggac accagctgca gtactgatcc ctccaggaat
     3061 gattggaata actacttgca ggatttggat gaagacaaaa ataatcggtc ctaaaatgat
     3121 ggaatcacct actaatcctc ttaacgcatt caaatctttg aaaacaccta aattaataaa
     3181 ataaatcgtc agcgcgattg ataggcctaa tccgataatg gatatgaaat ttatcagttt
     3241 tcgagaggca gcaatattca ttattcatca actccttaga ggttctttgt taactatact
     3301 ttactagaaa agcaactaac tgcgccattt tttgatatga cagaaaacaa acaattttgt
     3361 aagttcatca taagtataaa agattgatct caaaaaaaca tccaactaaa gacttattta
     3421 ataacttgt
//
LOCUS       DASJUF010000186         3406 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.193, whole genome shotgun sequence.
ACCESSION   DASJUF010000186 DASJUF010000000
VERSION     DASJUF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.193"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            155..1195
                     /locus_tag="U4B62_002831"
     CDS             155..1195
                     /locus_tag="U4B62_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN2142927.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2019..2351
                     /locus_tag="U4B62_002832"
     CDS             2019..2351
                     /locus_tag="U4B62_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN2142928.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2970..3311
                     /locus_tag="U4B62_002833"
     CDS             2970..3311
                     /locus_tag="U4B62_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN2142929.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1266 a          460 c          646 g         1034 t
ORIGIN      
        1 aaggtagtac ataattcatt ttatttctga ttttaggtag tatttaatta aaaaacagtt
       61 ttaatgcgtt tgcatttttt atttatcttt gctattttcg aaaagagtag gtaattttac
      121 ataaataaaa caacgtgaaa gtagaggatc tacaatgact gattttaaat tttttaaggc
      181 ggatagagtt tacaacgaat tattttatca atttccaaaa gtctttattg tttctgacga
      241 atacaaaaaa atgaaagatt caactaagat tgcctatatg cttttaaaag caagattaga
      301 gatcgcaatc agcaaacggc aaatcgatga agaaggtaat gtttatttta cttatacgac
      361 aaatgaacta tgtagagtat taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga
      421 gttggaatcc tttggtttat tattacaaaa gcagatggga tttaacaaac agttagggaa
      481 aaataatcct aatagactat atctagcaga attaaaagtc tcagaaaatg atatctactt
      541 actcgaaaaa tttgatagag agaataggga aaacgttgat aaatcagagg gtatgaaaat
      601 catacccacc ctcgacgaaa aatcagacgc tgaatccctt ggggctcaag agggtatgaa
      661 aatcataccg tgccaaaacg ttgataaatc agagggtatg aaaatcatac cagaacttaa
      721 taataatata ttagacacta atagacacaa tatagacact gaaaaagacc gcctacaaga
      781 tcaattgttg ttagacaatt ttgagacaat tatgacaaac gacagcattg ctacgtttgt
      841 ccctgaacga gtattaaatt tgataaaaac attttcttca agttacagtg aagctcaaaa
      901 aaccgtccag actattcata atgcaaagaa aaaagctgaa atagaaagtg atatttcgat
      961 agtttttgaa gaactcgata gttattatgt caatgcagaa caagaattat acacgacact
     1021 gttaaaagcc tatcaaaaat taaaaaccga aaaagtcgaa aatatccaga acctgatttt
     1081 tgtctatgta aaaaattggt ttatcgaaaa accaatagct gctaaagtat caagtgaaaa
     1141 acgtttgaat tatgcaagct ccccaagcac tattacgaaa gactggttag agtgaaactt
     1201 tactgacaaa gtgattgttg cttcatggag taacattaaa attaaaaatt ttttattatt
     1261 ctaattatat tacgaatatt acaaaataat agctatttta atagaattta taagttaaaa
     1321 tattatgata aaaatcacaa acgtagcctt ttacgtcata agtgttaaaa gaaataatta
     1381 atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga gttataataa
     1441 tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata ttacttcttt
     1501 tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg tctgctatgc
     1561 ttgtgatgaa ttgattttcg ttttgcactt ttggatgata aatttggaca attgaaaata
     1621 caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag cgttcataca
     1681 cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat tacccgcaat
     1741 ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg ttaaggttaa
     1801 aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa tgtccgtgtg
     1861 gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga tgcgttccct
     1921 cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac
     1981 ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca ggaattttga
     2041 gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga
     2101 aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag
     2161 acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata attttaaaag
     2221 atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg
     2281 tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt
     2341 tgatcggctg aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa
     2401 atattgtcga gacttctaaa ggaacttaga gtcactagaa aaataaggag tagacttgat
     2461 ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt aacgtaaaaa
     2521 attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa
     2581 taagcgcaga gtaaacaaga gggaatttag aagttagaca agttagaata gcgtctagat
     2641 taatcatgag cagctactaa gggatctaga ctttgacgca aactcatgga ataaggagta
     2701 ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag
     2761 atacttgcga ggacgttgat tattggatca atcgagcatt taccgctgtc tactagggtt
     2821 tctcgtttcg gacgccataa acgagtcgaa attttttatg tatttttaag taaacgaaaa
     2881 acgataaaat atgaacaagt cctgaataaa gtgaaaaaat taatataaag catagagaaa
     2941 atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat atacgattgg
     3001 gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt gtgacgttta
     3061 gaaggggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat gatttatacg
     3121 atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat gatgaaggta
     3181 atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct gttgaagata
     3241 cttggtcata tttgagtgat tatattgtca gctttgaaat cattgaattt gtagcggata
     3301 aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag gagtcacgac
     3361 gattaataaa aaggtagtat taccttaggc attgagtaag gaattt
//
LOCUS       DASJUF010000187         3381 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJUF010000187 DASJUF010000000
VERSION     DASJUF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.194"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            260..523
                     /locus_tag="U4B62_002834"
     CDS             260..523
                     /locus_tag="U4B62_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN2142930.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            517..867
                     /locus_tag="U4B62_002835"
     CDS             517..867
                     /locus_tag="U4B62_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2142931.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(891..1505)
                     /locus_tag="U4B62_002836"
     CDS             complement(891..1505)
                     /locus_tag="U4B62_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2142932.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1818..2240
                     /locus_tag="U4B62_002837"
     CDS             1818..2240
                     /locus_tag="U4B62_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142933.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2250..2453
                     /locus_tag="U4B62_002838"
     CDS             2250..2453
                     /locus_tag="U4B62_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142934.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2664..3248
                     /locus_tag="U4B62_002839"
     CDS             2664..3248
                     /locus_tag="U4B62_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2142935.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1166 a          491 c          602 g         1122 t
ORIGIN      
        1 tccattttgt atcaataact atgaaatctt taaagtatcc agaagagatt atttttagat
       61 caattttttt cttacctgat tctaaagatt ttgttttctt aaacatatta tgatttcctt
      121 tcgactattt tcatataaat gttaacatag tttatggatt catggaatgt taatctaatt
      181 taatagaaaa ggataattaa gtatatacgt tttttcttat gtatgttata ctatgtatat
      241 acagaaagag gtgtctacta tggaaatgtt acctataaaa aaatggggaa atagtaatgg
      301 ccttagattg ccaaagtata ttatggaata tcttggaatt catacagaag acaaagtgaa
      361 gataatacaa gaagaagtaa acggtcagaa gcggctaatt atcgaagcgg ctaatataga
      421 aaatgaatta acaattgaac agttatttga aaattacact gatgaaagaa atcatgtgac
      481 gattcaaaat ttaggtgaag cagtaggtaa tgaaaaatgg tgagaaaacc tagacaaggc
      541 gatattctat tattgaatac agcacctcga tctggacacg aacagacagg taaaagacct
      601 tatattgttt taagtcatga tattattgcg gattatagta acgttgttac agttgctcca
      661 atttctagta caactagaaa ctatccgcta tatgtaaata taaactcaga atatgaaatg
      721 aagacaacag gaaaagtatt attagatcaa ctaacgacaa ttgattatga agccagagag
      781 tgtatttttt tggaaaaagc gcatgatagt ttagttgagg agttattgat gaaagttaga
      841 acagtttttc aaaaagtaag caaataaaat agaaacagca gcatgatcaa ctattcgtga
      901 ttgaccatgc tgtctttttt tattctatat acggtttgtc tcgtaacatt gtagtctttt
      961 gcaatcttag cgatcgcaac accttttttt agatcctcaa cgatgttttt ataaacaaga
     1021 cgacgttggg gatcttttgc attggcactg tatagcttag gacgtccttt ataaacaccg
     1081 ttcgcttttg caatcttaat gccttgtgcc tgacgtcgtt tagattcggt acgttcttgt
     1141 tcagcgatca tggccaaaat ttgaatgatc aaatctttaa tgaatttatc caggagagga
     1201 ttgccaattg cttcggccat gatcggtaga cttgtgatga tcagctgcac atttttcttc
     1261 ttcaagtaat tgactgaagc gatgatctcg tcgtaatttc ttccaagtcg atcaatagct
     1321 tccacaataa aaatatcttg atctcggacg aaatccagtg cttcttgaaa aacaagtcga
     1381 tgagtaatcg ttgcgccaga ttgtttctca atgaaaattc tctctgcacc gaattttttc
     1441 atttcttcaa tctgccgttg ttcattttga tcgatggatg agacacgtat gtatgcaact
     1501 ctcaaaattt ttacctactt tctgcaacta tttaaaaaac accctaatca tacacgagaa
     1561 aaccagattt ttcaatgtcg cggtcgtgtc tagttagggt ataccttata tagacaaaaa
     1621 ctatgatgga ggaaatagta tgtgtcactt ttctttttaa atggcaatat tttttgtaaa
     1681 taaatgtcaa tcaagaaaat ttaaacgatt atgacattaa aaatatattt tatgaatttt
     1741 ctagtatata acttgattta tctgttaaga tttattaaga aaacgttttc ttatttttga
     1801 tttcgaggtg atagacaatg aaaaataagt ttattttatc tttaggtatt ttaactattg
     1861 gaattagtgt aactggaact acaattccta catttgctga tagtacaaat gaaccaacta
     1921 ctgtagtaga taataaccaa gaattaactc ctttagatag gaacggaata gccattggta
     1981 acccaactaa cgacttaact gttactgaaa attatttaat agtttctcca ttacttgctc
     2041 gagcagcttc taaaacgata cgagtgacag gaacgatgat ttttgataaa gttggttggc
     2101 cttctagacc tcctcaatca cggaattgga aagaaaaaag aaatggttat tggtactatg
     2161 gaaaattgta tttacaaagt tataaatcag ctggaaacaa ttggattgcg acttattcag
     2221 gaaccttaac tagaaagtag ggatttttta tgccagaatt tgtgaacaca ccaaattttt
     2281 atcaagatag cgatattcta agtaaaatta atttaccgaa agaagaatca gctttacaag
     2341 ttcagcgaac tttacaggag acacaaaaaa ttcaaaaatc aatttctaat ctagaagatt
     2401 ctgaagaaaa aagaaaaaaa agagaccaaa aaacatatca aaaagaaaaa taatgatttt
     2461 aaaaaaagag tgtctagtcg acattctttt tttctaattg aaagtgttca taaaatcacc
     2521 catttctgga cactttcttt ttttgcttta tttttcaaaa aaacaattca tttcatgttc
     2581 ataaacccat aacaatatct atgttcagtt aaactatatg gataaagtta tgttacaata
     2641 aaattaatga aggagtgatt tttttgaaaa taggatacgc ccgggtatct actggtttac
     2701 aaaatttgga tttacaagaa gacagcctaa ataaatttgg ttgtgaaaaa atttttactg
     2761 atcatatgag tggggcaaaa agtaatcgac caggattaga aatggccatt gattttgttc
     2821 gctctggcga tacacttgtc gtttggcgct tagatcgttt aggtagaaat atggaagatt
     2881 tgatttcaat cgtcaatcga ctgaatgagc gtggtgtgag ttttcatagt attcaagaaa
     2941 atattacgat ggacaaatca agttctacag gacagctcat gtttcatttg tttgcggcat
     3001 ttgcagaatt tgagcgtaat ttgatcctgg aacgatccgc agcaggacga gcagctgctc
     3061 gtgcaagagg acggtttggt ggtcgtcctg aaaaattgtc tgaacaagat ttggaattac
     3121 tgaagacttt agtggatagc ggtacaccta ttaagactat tgcggaacgg tggaacgtct
     3181 caagaaccac catttatcgt tatttggcaa aaatggagga gaaaaatgga aagacaaatt
     3241 ctcattagtt tttgtcattt aatgctgaaa atttttgcca tttatctaga aaagtgacaa
     3301 tagtatgaga attttttgta agtgtctact gataacttat tctcaaatgt tgtaaggatt
     3361 ttattcattt gttaatatta a
//
LOCUS       DASJUF010000188         3359 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJUF010000188 DASJUF010000000
VERSION     DASJUF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.195"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            96..1175
                     /locus_tag="U4B62_002840"
     CDS             96..1175
                     /locus_tag="U4B62_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2142936.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1476..1718)
                     /locus_tag="U4B62_002841"
     CDS             complement(1476..1718)
                     /locus_tag="U4B62_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2142937.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(1750..2307)
                     /locus_tag="U4B62_002842"
     CDS             complement(1750..2307)
                     /locus_tag="U4B62_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2142938.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(2320..2508)
                     /locus_tag="U4B62_002843"
     CDS             complement(2320..2508)
                     /locus_tag="U4B62_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2142939.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(2521..3087)
                     /gene="amaP"
                     /locus_tag="U4B62_002844"
     CDS             complement(2521..3087)
                     /gene="amaP"
                     /locus_tag="U4B62_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2142940.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT          927 a          767 c          679 g          986 t
ORIGIN      
        1 taattaactt agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag
       61 ttaccacaac tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag
      121 tttcacgcaa gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa
      181 aggaaaacta ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa
      241 acaacgaaat caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg
      301 tctacgacta ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga
      361 accgcttgaa ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg
      421 cggacgacat gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa
      481 aacacgaatt gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg
      541 accgtggcat catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga
      601 aatcgtcccg tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc
      661 gcccggaggc ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag
      721 gaaacaagac caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg
      781 aggtcattct gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc
      841 tgagagaacg ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg
      901 gctcggagtt cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc
      961 acccctatgc ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt
     1021 tcatccccaa gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc
     1081 gttggatgaa cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg
     1141 tccaggcatt taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct
     1201 ccctcggcat ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat
     1261 gcctcagctc attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga
     1321 gcaaccgggt caccatgggt ggctaactta aacttgaaat ttacgtaagg aataaagaat
     1381 acttcatata aaatatataa aaaagaaccg cagcgagtgt cgctacggtt ctcaaatttg
     1441 ttcttcattt caacataagt ttttcttcaa tttgcttata atttacgtgc aaagaatgaa
     1501 acgactaata ctaaaatgat tgcaccgatc aatgatggaa tgatagccat tcctgcaatt
     1561 gttggtcccc aagttcctag taaagcttgt ccaattgctg aacctactaa accagcaatg
     1621 atgtttgcga tgatccccat tgatgatcct ttattcgtga ttgctcctgc aattgctcca
     1681 atgataccgc cgacgattaa tgcccaaata aatcccattt taaattcctc ctaactgact
     1741 tcttacttct tattgtacac gtggttcgtt attttctttg actttttgtg cacctttgcc
     1801 tacagcagat tttgctttat ctgtttggcg tgaagcaaat tgtcctgtgg attttgctgc
     1861 ttctgttact ttgtcttgta ctgtttcttg atctttttgg tattcttctt gtgttttgat
     1921 gtctgcaaca ttcacattca cttcgataac atctaaatgt gtcatattgt ttacttcagt
     1981 actgatcaat tctttgattt ggttgtaaat atcttcgatg tctttaccgt attctacgat
     2041 cacatctaga tccacagcta cttgtttctt acctacttct gtattgatac ctgctgttac
     2101 gttatctgtg ttgactaatt tttcagccat atttgagaaa aatccgccat ctaatgtcaa
     2161 tagaccatct acatctttca atgcataacc gatgattttt tggatgactt tgtcttcata
     2221 agtcaattca ccattcatca tgcggttttc tcctttttca tttgccaaaa ctgatccatt
     2281 tgttgtattc atgtttttgt tttccatctt tgtttcctcc tatgatttaa aaaattgatc
     2341 aactattcct gactgtttca aataaaaacc gattcctgcg ccaattgctg tcatgattat
     2401 gatcaacaat gtcttgaaaa atccaatcgt taaccataaa acagctagca gtagtccaag
     2461 acccccaaag ataataggga gcttgtatgc tgaaaatagt tctttcatta tttggccctc
     2521 ctattccact cgagagtgct tgtagtctct tgtatcttct tgttcttcat aaccgctgta
     2581 tgtgactgcc actttgaccg tttcttttgt tccgagtact ttagttactt cttcttcgat
     2641 atcttgcatc aatacacctg ttttccctac taatgaagaa gttcgtttca agtctccgtt
     2701 gacatggata tggattcgat ttttcgttga acgtatccgt actttaggtg aatgaatgaa
     2761 ctcttcttcg tgtaaatggg aacgtaccaa cccttcgatc gcttttttgt cgattgttag
     2821 tttgccatct tcccgtttca taacgaaagt tcctcttgac tttggataga aaagaacgac
     2881 tagcaagatg ataatagcaa gaacggcaaa tactacagct gcccagaaaa tatattgttg
     2941 catgtagtaa ttcgttaaag taaagaagcg aaatcgttcc aattgttcag gcataatata
     3001 cggcgcattg atcaacgcaa tcgctagaaa tactgaaagt agcagtaaac tcatgataac
     3061 accgattatt tttaacccct tattcatttt ccacctccta taaacttatt tttaccctgt
     3121 aaataactct accgacaatc acagtataga tagcgaacag ttagcatagc atcagtaaat
     3181 aaagcgctac caaacattgc tggatcataa cggcgtttta ttgccagtct gatactgaaa
     3241 taacaattgc ttacttattc tctatttact tttgattgct ggctatactt aaaggatatt
     3301 attcaagatt aaaaattgca aagattatgc tcagttatta ttacaagatt ctatttatt
//
LOCUS       DASJUF010000189         3305 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJUF010000189 DASJUF010000000
VERSION     DASJUF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.196"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..741
                     /locus_tag="U4B62_002845"
     CDS             <1..741
                     /locus_tag="U4B62_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2142941.1"
                     /translation="KYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNI
                     KDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLIN
                     GVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQS
                     KLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVR
                     KITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            731..1471
                     /locus_tag="U4B62_002846"
     CDS             731..1471
                     /locus_tag="U4B62_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2142942.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1468..>3305
                     /locus_tag="U4B62_002847"
     CDS             1468..>3305
                     /locus_tag="U4B62_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2142943.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1244 a          402 c          636 g         1023 t
ORIGIN      
        1 aaatataatg ccattactac ttggaataat attaaacagt ttttcaaaga tacttggaaa
       61 aatattaaag atacggcata caatacgtgg atttctataa aaaataccat gattaataca
      121 tggaataaca ttaaggactc tttctggaat attgttactg gaattgttaa ttctgctgaa
      181 aatgcatgga caaatctaaa aaatggtgtt tcaaatgcta ttaatcgggt aaaagaaatc
      241 tttaattcgt taagggaagt caatttattt gaaattggca agaacatcat tgatggactt
      301 ataaatggtg taaaagaaaa gtggaatgct ttgaaagata ctatcaaagg tattgcaggt
      361 aatatcaaaa attggattaa aggagcttta gatattcatt ctccatcaag atggatgcgt
      421 gatatggttg gtaaaaatat tgttcaaggt attatcgttg gtattgataa agagcaaagt
      481 aagttagatc aaaccatgac aaatttagta aaaacaccgt ctgttcaacc tgtagtaaat
      541 ggaataagtc acctaccaat tacacaaaca aagcaaaata tatctgctag tgaaacaaaa
      601 gaaattcatt tacatttaaa tgtttatgga gatttacctg attcaatggt tcggaagatt
      661 accacaaaaa ttaaaacaga attgacaaga caaatgaaac gagatgctgg tgcagtagga
      721 ggaacattat atgcaactta agagaggaca attttttatt aatcaacatt attcttctga
      781 atttaatgtg tatattcaaa acaggcctgc ttctgtttca gctagtcgag taattgaatt
      841 gagagagcga gaaggaaatg attctatcat tattgataaa ggctattaca aaaatgtgac
      901 tagaaagatt gagtgttact ataaagcacc ttcaattgat atggtgcagg agtgggaaga
      961 tcgaatcact gaatggttag atatgtcgtc ttatagtgat tttattcttt actatgatga
     1021 acaatatatt tatcaagctg tagtaataga aggacctgaa tttaaaggaa caagaaaaac
     1081 aggaaatatt gtcccttttg aatttacagt gagtattcga ccatttaagg aaaattacaa
     1141 tggtagattt actattcgac aaacagaaac ttttgaaatt tataacccag agaagtattc
     1201 ttcaaaaccg cttattaaat tgagtggttc tggagatgct tctttttata ttaataagga
     1261 taaatatgat ttgaaatcgt tggacagaga attatttata gattctaaat tagaagaggc
     1321 ttatcgaaaa ttagatggta atttagaaca tcaggatcag gtcaccttat ttttagattt
     1381 tccattttta tatcctggaa aaaatgaaat taaatggacg aaaaatattc attcatttga
     1441 aataatgcct aggtggtgga gaaaagtatg aaaccaagaa tatatagtcc tactgaaaca
     1501 gatttctcaa cgaatggctt aggaatttta aaggattgta caagatgtgg aatatatgaa
     1561 gtagctaatg gaaaatatga attagagttg gattatcctt taggaactag atttgatgaa
     1621 tattttgaaa atgactatca aataaaagca aagccaaacg atcaagaaga gtatcatatc
     1681 ttttttattg atgataaaga tatagatact tttttaaata cagtaactat ttatgctcag
     1741 agtcgtacaa atcgacttgg aagacgggta gtcactcttg tggaagtgga ctctaaaact
     1801 ggtcaagaag cgatatcaat tattgaaact aaaatggata aaaaatctga catacgactt
     1861 tattctgata ttacggctgt ttctagcaca atctttgaag cgagaaatgt tttaaattgt
     1921 attgctggtg aacaaggatc attgcttcaa tattggggcg gagaaattaa acgtgaacca
     1981 tttaagcttt ctttgttaaa gcgaagaggt cgcaataatg ttggaacgat tcggtatgga
     2041 aaagatatgt ctggtttgaa ggtcaaatta gattggacag gtataaaaac aagaattatt
     2101 ccatatgctg atcctcaaag tgaagtaggt acgactaatc gaatttatgg ttcgccagta
     2161 gatagtgcct atattaataa ctatcctgat gtctatacag aacatgttca gtttacagaa
     2221 gaacaaggag taaaagacat taagagctta aataaaatag ctaaaaatta ctttaaaacg
     2281 atcaatcctg gctgtgataa gcctaaagtt tctattacgg ttgaatttga taagttgact
     2341 gatagtgaag aagcgaaaga atttgcgaag attagaaatt atggtctgtt tgatacattt
     2401 aaaatatatc ataaaaaata taatatttat cttgaatcaa aggttagtgg actacaatac
     2461 gattctttat ctgaaaaaac tttgaaatta gaagcaggag acattcaagt tgctttttat
     2521 cagcaacaag ctgtaactat tcaggataaa ttaaaagatt atgcaacgaa taattatatg
     2581 agtgatttta atgattatgt ttcttcaatg attacaggtc aaggcagtgc aggaggatat
     2641 gtagttttat ggccaaaaga aaaaccttcc aacatcttta ttatggataa tccagattta
     2701 gaaaaggcaa gagaagtact aagaatgaat aaaaatggga ttgctttttc taagaaagga
     2761 tggcgggggc cttttaattc agcttggaca ttagatagta tatttaatgc caattttata
     2821 caaactggat taatcaaagc agatatcttt caaaattctt ttaacaagac tggagatgta
     2881 ttgaaattag ttaatggact actccaaatt tggaacaaca aaaagaaaat aatggagtta
     2941 actaaaaaag gaatggaatt ttggaattct aagggttcaa ttggaacgat tggaacaact
     3001 gattctgttg gtaatccttt tcctggggct tctactccaa cccctattga agataattct
     3061 ttagttattc gtacaaatgg agacggcaaa tatattttga tttctcctaa agtaggaaaa
     3121 ggattagttt tattaggaaa tggtaaagca atttattttg gagacttaga tgtacaagga
     3181 aaacttacag ttaaaggaaa agaaataatt cctggacaag gtggtggtcc atcgggaggt
     3241 ggagaatcta ctggtgggta tcctagtgaa ttaaaaacag atgcagaaaa gagagcttgg
     3301 agaat
//
LOCUS       DASJUF010000190         3291 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.197, whole genome shotgun sequence.
ACCESSION   DASJUF010000190 DASJUF010000000
VERSION     DASJUF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.197"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="U4B62_002848"
     CDS             complement(<1..2232)
                     /locus_tag="U4B62_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142944.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="U4B62_002849"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="U4B62_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          898 a          736 c          535 g         1122 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat t
//
LOCUS       DASJUF010000191         3201 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.199, whole genome shotgun sequence.
ACCESSION   DASJUF010000191 DASJUF010000000
VERSION     DASJUF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.199"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="U4B62_002850"
     CDS             301..585
                     /locus_tag="U4B62_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142945.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="U4B62_002851"
     CDS             588..1004
                     /locus_tag="U4B62_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142946.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="U4B62_002852"
     CDS             complement(1141..1860)
                     /locus_tag="U4B62_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2142947.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2514..2609
                     /locus_tag="U4B62_002853"
     CDS             2514..2609
                     /locus_tag="U4B62_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2142948.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2912..3157)
                     /locus_tag="U4B62_002854"
     CDS             complement(2912..3157)
                     /locus_tag="U4B62_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142949.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaaa ctatgtcctt cgttctaaag cgccttacag
     2401 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2461 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2521 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2581 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2641 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
     2701 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2761 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2821 gatttaagac gttttattta gtttttggtg tattttcatt ggtccaagct aaaaaactct
     2881 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
     2941 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
     3001 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     3061 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     3121 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     3181 ttctttgccg tctatactct c
//
LOCUS       DASJUF010000192         3074 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJUF010000192 DASJUF010000000
VERSION     DASJUF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.200"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..518
                     /locus_tag="U4B62_002855"
     CDS             123..518
                     /locus_tag="U4B62_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142950.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            518..769
                     /locus_tag="U4B62_002856"
     CDS             518..769
                     /locus_tag="U4B62_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142951.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            812..1207
                     /locus_tag="U4B62_002857"
     CDS             812..1207
                     /locus_tag="U4B62_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142952.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            1204..2460
                     /locus_tag="U4B62_002858"
     CDS             1204..2460
                     /locus_tag="U4B62_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEN2142953.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            2472..2795
                     /locus_tag="U4B62_002859"
     CDS             2472..2795
                     /locus_tag="U4B62_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142954.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            2788..3054
                     /locus_tag="U4B62_002860"
     CDS             2788..3054
                     /locus_tag="U4B62_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142955.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         1198 a          474 c          651 g          751 t
ORIGIN      
        1 aaaaaagaaa gtagaaaagt agataagtaa ctctaaaaaa tccaaaaaaa tccaggctcg
       61 attgcgtact tatcttaaag gagaaacaat ccatcatata taacccgaaa ggagacttct
      121 atttgcctaa gaaaagacga aagaccatag tcatcatttt gaccattctt actataggaa
      181 tcggagggaa attttacatg gacaaaagag aaatgcaaag acagaaagat ttactcgcag
      241 tagaaaaaca gtccgtcaaa gttctgaaaa atacctttgc ggatatcaag gaagtgaaga
      301 ttgaaaaatc tgctcgaaat gaaatgacgg gttcataccg gatagttatt ctaatgacta
      361 ataaacaaga tcaatcgatt tatttttctt atagtttttg gaaagaaaga aatgagatag
      421 gaagttacgg aatagttgat gaaaaaaagc aaaaagaagg aaatacgctc aataaagtta
      481 aagtaactta ctctaatgga aatgaggaaa gtatttaatg attactgaca aacaatatag
      541 tgaattaagt gatgctgttt actggctaga tcctaaacat agagactatg tacctgaaat
      601 gcaagaaaat ttaagtttta aaattaacgg cactatgtat aaaatcctaa aaatcaaaaa
      661 ctccttcgac ggcatgcagg cgatggctgt agcgccaatc gtccatagca aattggagaa
      721 aaatttcaaa aataaaaaaa ttcctgcaaa ctttcgcgta cttaaataat gaaggtgtca
      781 atacttacat aaaaagaaag gaggcacgtt cttgtcaagg aaaataagaa aactcagctt
      841 agccatttta ttaatcgtca tattagggat cggagggaaa gtctatatgg acaaaagaga
      901 agcacaaagg cagaaagatt tactcgcagt agaaaaacag tcagtcaaag ttctgaaaaa
      961 tacctttgcg gatatcaggg aagtgaaggt agaagaattt aaaaaaaacc ctgttaccgg
     1021 aagttatggt gcacttgtga caatgacaaa taatgaaggg aaatctgttt attttgatta
     1081 tagtttttgg aaggagagaa atgaacttgg tggttttggc attgagaaca gagcggtaca
     1141 aaagacagga gtcactataa aaagagtaaa agtaattttt tctaacggac aggaggagtt
     1201 agtatgacaa taaacgataa gcattacaat gatattagtg agaaggttta ttggctagat
     1261 cccaaatatc cccgctacaa tgagggatac aaaaagaact ctgttaaaga atttgctggt
     1321 atggaatttc aaatcctaca aatcaaagac tcattggacg gcatgcaggc gatgtctgta
     1381 gcgccaatca agaacggtaa agtggatctt tctcaagtag tgattgcttt tgcaggaacg
     1441 aacttaagtg attggaaaga tttggaaacg gatgcaagaa gtatcatgaa tctctacggg
     1501 aatagaccag gagcaagttt ttctgaaaaa gcaacaacaa acaagatttt atccgttagt
     1561 ggattgaaaa cagcggggca aatcaattca gcgattgcat ttgctaatga agtaaaaaag
     1621 gaatatggag aagctgctat ttctactacc ggccattcat taggtggatt cttagcatta
     1681 tatgtagcag cagagaaaca atggaaaaat gtcggcttca acgggccgga cccgtatgat
     1741 ctcttatcac cagaagcaaa aaaatgggtg gaagaaaatc ccggtatgct caccaattac
     1801 cgaaatcacg ccgattttat cggtaatttt ggtggtaatg gcacaggtgc tgaagttcgg
     1861 atcagtatgg aaacagcatt cttgaatctg cttgcttccc acagtttaac ctcttggaag
     1921 tttgatgaaa atggaaagct gattattcct gaaaatgaaa acaatgttaa agctcgaaaa
     1981 caacagaaag aaaaccaagc gacccagttg ttttcctatc agttgcaaga actgaacaac
     2041 atacgtaaaa aactacgatt aagtggcggc cggctgacag ttaatgaaca aatttactta
     2101 gatgacacac aagctaggtt agtagtggaa aagttatcgg caaaaatgaa aagcgctatg
     2161 gaagaagtta tccgcatcaa tcaaagagga atttttgagc tagagaaaaa atggcaagaa
     2221 ggcttgcggc aagcacgtgt tgtagcccct tcgttgactg aacaggaaat catagaagca
     2281 ttagctagtg tgggcgccac aaaagaaaac ttagtcgatt ctgcgaaaga agtctatctg
     2341 ctacgaatca ataaggcaag acgtatgggg gaggcctttg atacattggc gaaggagatc
     2401 caagacaaaa tcaatgaaat cgtggcacga gatcgagaat tagcacagca attcaattaa
     2461 aggagaagta gatgaaacta gaagaattgg aaagagatta tcgaagtgaa cgaagacaat
     2521 tagaagctca agaagatgaa ttgatccact ttaagaatca aggattacga ttgatggagc
     2581 aagaggagca gcgactgcat catgtattaa agaacttagc tttagataat gagcctctgt
     2641 ttcaagcaag acgaagttat tcgaattatc aggagcatta tcaacaagcc ctacaagtag
     2701 aacgacgaaa actctatcag cagcaagatg aactagaacg ttcctatcgt caaaaaatca
     2761 gacaaataac tatggagaaa tcatcttatg aatgaatttt taatgcaatt actaaaagaa
     2821 ttagagaaaa atctacatcg atcaaatgta cgttcagttg gtatggggat atctcagcaa
     2881 atttgggcag aaaaacgatt gacagatcaa aactaccaaa tgactgcaga agaagctgct
     2941 caagaatttt ctaaccgaca tgtacacttg aaaggatcag taaaagggca atttgcagaa
     3001 aaactaactg aattattcca aagacaagaa aagaaattaa aacagtttcg ttagtaaagt
     3061 tatatagaaa aatt
//
LOCUS       DASJUF010000193         3072 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJUF010000193 DASJUF010000000
VERSION     DASJUF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.201"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..1103
                     /locus_tag="U4B62_002861"
     CDS             288..1103
                     /locus_tag="U4B62_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142956.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1229..1486
                     /locus_tag="U4B62_002862"
     CDS             1229..1486
                     /locus_tag="U4B62_002862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142957.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1738..2310)
                     /locus_tag="U4B62_002863"
     CDS             complement(1738..2310)
                     /locus_tag="U4B62_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2142958.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2326..2931)
                     /locus_tag="U4B62_002864"
     CDS             complement(2326..2931)
                     /locus_tag="U4B62_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN2142959.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1019 a          539 c          473 g         1041 t
ORIGIN      
        1 aaaataagaa attcaaaaaa gaaaaaactt tccttaaaaa caaaaaatag gagtattcag
       61 tttcttaata gttttgtaat agttgtatgt agaagttttc ttaatagttt ttgtaataat
      121 ttattaatcg tagtttatat aacttatttg taagtgacta gagaattaaa taaaaaataa
      181 ttactatcta gtacgaaatt aattacatag gataatatat taaggtgctg gactacaagt
      241 aaaaaatact gtatgatcat gacagattca agaaaggagc aataagattg gttaagatag
      301 caggactttc taaaggtgga aaaaccgttt cacaacttta taaacaaaac tacagagact
      361 ggagacagtt cagaaaaaac attaaggatt cttactttat tctaccaaca gaacttgaaa
      421 aatatttacc gaataacaaa gctattaatt tatatctgta ttactgtttt aaggcaaaga
      481 atgaaggcgg ggattcctgg cattcggtca ataccatagc agagtctcta ggagtgacaa
      541 ctaaaactat aaacacctgg aataaaacct tagttgattt aggtatgatt gcacgtatag
      601 acgataacca tctcagtaaa tcgacaattt tacttcctct ctctagttac tataaattag
      661 cccttgatag ttctttaaag gatgttaccg agagcactgt ccatgaaatt gatggcaact
      721 tggttgcagt ttatcatttg tttgaatgga gaaaaaatga ggctgatgaa aattataacg
      781 ttccttataa cacactctgt tgtgttttta aaagaaaaac acaaaaagat actatctata
      841 aatttatctt ggtccaagat gagaaaataa gtaatttcga attaagcact ccttctagta
      901 agatttatga cgatgcatat agatttgaat gtcaagatct agaagccctc ctttccgcaa
      961 ataatctaaa caatatagaa catgaaaact ttgttgtaaa cactagattt aatttagttt
     1021 ctgaaaagga ctcagatttg ttagatttgc tgatagagct tactaaaaat ctagataaga
     1081 aagaaaacat tactgtacta taactagcat cggtccatca ttaagtttgg actattagaa
     1141 cacttgctat tacagaaaaa ggtatgctag ccacgcttac tatttattgg tatcaaacaa
     1201 gtatcttaat ttgaaaggaa gatgatttat ggctaattta tatatagaga acaaaggaga
     1261 cttgaatgcc attattactt taatcgtagt tatggtaaaa tcaataggta atatcacaat
     1321 aggaatgctg tttattatat tagcagcagt gctgattttt atgatagtta tttggttctt
     1381 aaatgaaaga tctaaagaca atcaacaccg tagggatatg gagataaaat caatagagtc
     1441 aatagattat ttatcgataa taacactaaa aaagccacgt tcctaaacgt gacttacgct
     1501 gaacgatatg tgttgtttgg tagacgaaca aatcgttcat tataaatata tgatggtatc
     1561 ctaatatagt aagcgtggct agcatacctt taattacttt accttttaca gttaccatac
     1621 acattttttg agcagaaatc aagtaaataa aaaaatatta tatttcacaa catgctaata
     1681 gactcatatc tagaacgcat taagtattta cctgtctggt tcgctgagac ttctttttta
     1741 actttcttca attttcttct ttattctaaa aagcgtactg cgactgaaac cggttatagc
     1801 ttcaacttcc ttatacgatt tacctgaagt aagcagttca tatgcgtgcc tcttcttcgg
     1861 tgtgattgtt gctttcggac gtccttctct gaaattagga ttattctttc ttgcaaacat
     1921 ttttccttct tgggtacgtt ccacaatcat atcacgttcc atttctgcta cactcaacaa
     1981 cgtccgtgtc accattcgcc ccatggacgt attttcaatc gttcctagat tcaaaacctt
     2041 gatcgttacg ttatcatcga gtaaaggttc aatgatctct aatgcttctc tcgtgttacg
     2101 agcaaatcga tcaagcttcg taacaactat ttcatctcca ggcttcagac cagccaacag
     2161 ctgattgaat tttggccgat cccgttttgt acccgtaaat ttttctgaaa aaatattctt
     2221 cttctctacg ccgttctcaa gcaactgttg aatttgttct tttgtacttt gagaagcggt
     2281 tgagacacgt gcatatccat atttcgtcat ttggtccacc tacttttaca aatcatctat
     2341 cgtatactca gaataccctt catagaaata gctgacatcg attcctttca tatataactc
     2401 gcgatcgtca attttatcag tcagagcatt ggaaattaga tagcgtattt ccaaatcgtt
     2461 tatgggactt cgctccattg cagaaagata gtctgcttta tcgattaagt tccaatctac
     2521 aactttttgc aattcttcct ttaaaatgag atccagccaa attctagtac ttcggccatt
     2581 tccttctcga aatgggtgag cgatattcat ctccacatat ttcttgataa tctcttcaaa
     2641 agaagtttgt ggcatctgat caatatgctg taacgaatgt tctaaataca taactggtgc
     2701 aaatcgaaaa tttccttttg caatattgac cgatcggatt tttccggcaa aatcgtaaat
     2761 atccgaaaat agaaattcat gaatttcaga taaaccttta aatgtaccaa cttcgatttc
     2821 atttattttt ccacaatcgt acaattcttt cgctttttgt ttactgagtt tttcttcaac
     2881 cttagccaac tcaacttgat tggttaatcc caatttattc tctaaaatca tgcgaaatct
     2941 ccttaacttt atgacataag tttatgaaac actctcaacc ccattatatc gcgattttgt
     3001 ttatgtgtca aaaactatta agtttctgac ccccgataga tttttcaagt gtctctattt
     3061 tgcacagtct ag
//
LOCUS       DASJUF010000194         3022 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJUF010000194 DASJUF010000000
VERSION     DASJUF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.202"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..263)
                     /locus_tag="U4B62_002865"
     CDS             complement(<1..263)
                     /locus_tag="U4B62_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2142960.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAIL"
     gene            complement(281..601)
                     /locus_tag="U4B62_002866"
     CDS             complement(281..601)
                     /locus_tag="U4B62_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2142961.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            900..1676
                     /locus_tag="U4B62_002867"
     CDS             900..1676
                     /locus_tag="U4B62_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN2142962.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1691..1894
                     /locus_tag="U4B62_002868"
     CDS             1691..1894
                     /locus_tag="U4B62_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2142963.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1910..2248
                     /locus_tag="U4B62_002869"
     CDS             1910..2248
                     /locus_tag="U4B62_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN2142964.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2235..2414
                     /locus_tag="U4B62_002870"
     CDS             2235..2414
                     /locus_tag="U4B62_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142965.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2457..2927)
                     /locus_tag="U4B62_002871"
     CDS             complement(2457..2927)
                     /locus_tag="U4B62_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142966.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT         1109 a          463 c          530 g          920 t
ORIGIN      
        1 agaatagcta ttttatctgc ttctgtttca aattttgagt gaaaagttgt agaagtatcg
       61 tacaaaactg aaaattcaaa atgagaggcg ctgaaatgtg caagttcatg agataaatga
      121 aaggcttctg cagtttcacc gtatagattt tcattcaaaa aaatgattct gggttttgga
      181 tagtagaaac caggctcttt catctccata taaactactt tcaaattgta ttcgctcaac
      241 atttctttca atttcaaata catacaaacc atcactccaa ctattcattt tcctctaaag
      301 ctttagcaat tgcaatcgct ttacgcattg tctccttaga tatttctttt ccatcaaaag
      361 aaaaaacagt atcgtcttct gataaatcca catgtttagg ggtttctctt tcttctctac
      421 ctagaaggta gtctacagag acatcgaaat agtcagcaat ccgacttaat tcatctgaat
      481 taggagtatt atttttccac tttgctagat aaccgttaga atatccaaaa ttaatttcta
      541 actgccgtat agatatcttt ttcctcttcg ccaagtcttt tattatttca taggtattca
      601 tcgataaaac aacctttcta aatgtttaca aaaaaagttt agaaaaatac gcagaaataa
      661 tttgactaat tctgagtaat gagctatact atatcttgta aacagattta acaactaaaa
      721 agacaacaaa aaacactatt gatatgtaaa tgcagaccgc caagaaagct ttaaaatcaa
      781 tgtttttatg tcttatttaa ttatgctctt agtatagagt attactcaga tactgtcaat
      841 tgaatttaga aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata
      901 tgaacacacc gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg
      961 atgaaccata ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag
     1021 atgctttgtc gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag
     1081 gtcaatcaag agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat
     1141 caaaacttcc ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa
     1201 ttagaaaaca tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag
     1261 atacaatcat tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag
     1321 aacaaaagat tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta
     1381 cagattcagt aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga
     1441 ataaattact tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca
     1501 aaaaacacat gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg
     1561 gtggcactaa aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg
     1621 aattactaaa aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag
     1681 gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga
     1741 atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag
     1801 caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat
     1861 tatagattcg attatcacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct
     1921 agaagcaaaa atcccaattc cagaaggcta tgtaattatc tcccaagtgg attatgagga
     1981 gttaaaaaaa gctgatgata ctggtagatg gatgacgttg ccagaagtgc tagaacggat
     2041 taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat
     2101 tatcgatatc gagaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac
     2161 atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt
     2221 aaggaggcaa gcggatggga aaatttaata gagcgctagt gttcagcgca ccgctaatca
     2281 tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg
     2341 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
     2401 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
     2461 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
     2521 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
     2581 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
     2641 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
     2701 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
     2761 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata
     2821 tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt
     2881 tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt
     2941 gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag
     3001 gagagaagaa ataatgcaag aa
//
LOCUS       DASJUF010000195         3022 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJUF010000195 DASJUF010000000
VERSION     DASJUF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.203"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..962)
                     /locus_tag="U4B62_002872"
     CDS             complement(<1..962)
                     /locus_tag="U4B62_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2142967.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            complement(1261..1896)
                     /locus_tag="U4B62_002873"
     CDS             complement(1261..1896)
                     /locus_tag="U4B62_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142968.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..2644)
                     /locus_tag="U4B62_002874"
     CDS             complement(2009..2644)
                     /locus_tag="U4B62_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN2142969.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(2678..>3022)
                     /locus_tag="U4B62_002875"
     CDS             complement(2678..>3022)
                     /locus_tag="U4B62_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN2142970.1"
                     /translation="EKTIPFKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKD
                     ENTVMFTKKYWNEMENLKKYIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQE
                     EFDFKKKELLGL"
BASE COUNT          857 a          510 c          440 g         1215 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtc cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
     2581 caattaacat gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt
     2641 ccattaatag atcctctttc tcttttgata ttgaatatta taaaccaagc aattcttttt
     2701 tcttaaaatc gaattcttct tgattaataa tcccttcatc taacaactct ttatactttt
     2761 taatttcatc tgcacttgaa atttgtgttt ctttggaatt attatctaat atagattgtt
     2821 gcttctctat atattttttt aaattttcca tttcattcca atattttttt gtaaacataa
     2881 cggtgttttc atctttcgtg gccgctaata tacctccgcg actttcgttt ccgcctagca
     2941 aggtaaattg tatataacca ttactcatac ctggtttttt taattgtacg gctgatatat
     3001 ttttaaaggg gatagttttt tc
//
LOCUS       DASJUF010000196         3000 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJUF010000196 DASJUF010000000
VERSION     DASJUF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.204"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            168..2228
                     /locus_tag="U4B62_002876"
     CDS             168..2228
                     /locus_tag="U4B62_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN2142971.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2311..2880
                     /locus_tag="U4B62_002877"
     CDS             2311..2880
                     /locus_tag="U4B62_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2142972.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1088 a          537 c          567 g          808 t
ORIGIN      
        1 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
       61 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
      121 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
      181 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
      241 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
      301 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
      361 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
      421 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
      481 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
      541 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
      601 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
      661 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
      721 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
      781 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
      841 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
      901 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
      961 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     1021 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     1081 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     1141 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     1201 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     1261 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     1321 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     1381 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     1441 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     1501 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     1561 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     1621 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     1681 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     1741 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     1801 ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     1861 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     1921 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     1981 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     2041 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     2101 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     2161 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     2221 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     2281 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     2341 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     2401 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     2461 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     2521 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     2581 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     2641 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     2701 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     2761 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     2821 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     2881 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     2941 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
//
LOCUS       DASJUF010000197         2943 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJUF010000197 DASJUF010000000
VERSION     DASJUF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.205"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            196..2430
                     /locus_tag="U4B62_002878"
     CDS             196..2430
                     /locus_tag="U4B62_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2142973.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2578..2898
                     /locus_tag="U4B62_002879"
     CDS             2578..2898
                     /locus_tag="U4B62_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2142974.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         1047 a          541 c          596 g          759 t
ORIGIN      
        1 tcatatgaaa cgaattactt gcttcccttt ttatttttag tataataaga gtcaaaggtc
       61 aggaatggtc aatagagatt tcaccttttc tacctcataa atctcttaaa aaactagggg
      121 ttattctttt acctcatgaa aaacgagatg taaaatatgg atgtacccat ttttgtgaaa
      181 ggacgtgaat gattcatgct ttgtcaaaac tgcggcaaga atgaggcaac gattcacttg
      241 tatgccactg tcaatggtca gcggacacaa ctcgattact gtcagagctg ttatcaaaaa
      301 atcaaaaatc aacaaaatgg aggtctgact aatatggccc aaaatgatcc atttggattt
      361 ggcagtttgg acgatctttt ccgttccatg tctcgccaaa tgcaacagca aggaaattat
      421 gagcaaacac caccaacaca atttggcgga aacaacaact tcaatggtgg tcagccacct
      481 caaggcaata cagaaggttt attgggagaa tacggaatca atatcacaga acaagcacga
      541 caaggtgata tcgatcctgt gatcggacgc gatgacgaaa tcaaacgagt cattgaaatt
      601 ttaaatcgcc gaaccaagaa caatcctgtc ttgattggag aaccaggtgt tggtaaaaca
      661 gcagttgttg aaggtttagc acaaaaaatc gtagatggtg atgtaccgca aaaattaatg
      721 gacaaagaag tcattcgttt agatgtcgta tctttagtcc aaggtactgg tatacgagga
      781 caatttgaag aacgtatgca aaaattgatt gaagagatcc gtcaagcaga aaatgtgatc
      841 ctattcatag atgaagttca cgaaatagtc ggtgctggtt ctgctggtga tggaaatatg
      901 gatgctggta atattttaaa accagcttta gctcgaggag aattgcaaat ggttggtgct
      961 actaccttaa atgagtaccg catcatcgaa aaagacgctg ctttagaacg tcgtatgcaa
     1021 cctgtacggg tagatgaacc aactgtcgaa gaaacgatca gtattttgaa aggtttgcaa
     1081 aaacgttatg aagactatca tcatgtaaaa tatactgatg aggccatcga agcagccgca
     1141 actttatcca atcgttacgt ccaagatcgc ttcttgccgg ataaagccat cgatttgcta
     1201 gatgaaacag gttctaagaa aaacttgacg atccaaatcg ttgatcctaa aacaatcgag
     1261 aaaaaactgc aagaagcaga agaacaaaaa gttttagctt ctcgagaaga agatttcgaa
     1321 aaagcagctt attatcgcga tcagatcaat aaacttcaaa aaatgaaaga acgtcagctc
     1381 actgaagagg aaacacctgt gattaccgaa aaagatatgg aaaaaatcgt tgaacaacgg
     1441 acaggtattc cagtgggtga attaaaagaa aaagaacaga cacaactgaa aaacttagca
     1501 gatgacttga aagctcatgt catcggacaa gataacgctg tagatcgagt agcgaaagcg
     1561 attcgccgaa accgtgttgg attaaacaaa cagaatcgtc caatcggctc cttcttattt
     1621 gttggaccaa ctggtgtagg taaaacagag cttgcgaaac aacttgccta cgaactattc
     1681 ggttcacaag attcaatgat tcgtttcgat atgtctgaat atatggaaaa acatagtgtc
     1741 tctaaattga ttggttctcc tccaggttat gtcggatacg aagaagctgg tcaattaaca
     1801 gaaaaagtac ggagaaatcc ttacagtctt gtcttgttag atgaagtaga aaaagctcat
     1861 ccagatgttt tgcatatgtt cttgcaaatc ttagatgatg gtcgattgac agatgcacaa
     1921 ggaagaacgg tcagctttaa agatacgatc atcattatga caagtaatgc cggaactggt
     1981 aaagtagaag caaacgttgg ttttggtgcg gcacgcgaag gtgttactcg ttctgtattg
     2041 aaccaattaa acaactactt tacaccagaa tttctgaacc gttttgacgg gatcatcgaa
     2101 ttcagtgcat tatcaaaaga aaacttgatg acaatcgtca cactaatgct agacgatgtc
     2161 aaccaaatgc tggcagcaca gcaacttcac atcgaagttc ctacgaatgt caaagaaaaa
     2221 ttagttgact taggctatga tccatctatg ggtgctcgtc ctcttcgacg cacgatccaa
     2281 gaacaaatcg aagatggtat cgctgaattt tatcttgatc acccttctat ccatgagttg
     2341 aaagcaaaac tggataaaga tggaaaaatc atcgttactt ctaaaccaga acgtttggct
     2401 aaagaatcag ccgaagaaac ggctgaataa atccagtttt atagcgtgag acggtatttc
     2461 tttgagaaat gccgtctcat tcttttttca gaaaagtcaa aatactgttc attttataaa
     2521 aaagaaatta taataagaga aacaagcaat taaaaataca taaaagaggt gagcgccatg
     2581 aaattggtaa atgtaacaaa cagccattcc cgtcttgtat taaaccagct tgaaaacact
     2641 gatgcccatc tagtcaaggt ctacacagcc ggaaatacca ctattgtcta cactgaagcc
     2701 cctcaacata atgaaattct gctgatcaat gacaaacgta aaatccagcc gaaagaaatc
     2761 gaagatgcta aaaattattt cttagataaa atcaaagacg ccgtttacca cgaggatgaa
     2821 atcaaagtaa tcgaattggc aggccttgtt gaaatctcga ttccaaaaca tgaattaggc
     2881 gctacattag ccgtgtaaac aaattttgaa cataataatt aaagagcgtg ggacaaaagt
     2941 aaa
//
LOCUS       DASJUF010000198         2734 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJUF010000198 DASJUF010000000
VERSION     DASJUF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.206"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..521
                     /locus_tag="U4B62_002880"
     CDS             75..521
                     /locus_tag="U4B62_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2142975.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            794..2146
                     /locus_tag="U4B62_002881"
     CDS             794..2146
                     /locus_tag="U4B62_002881"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN2142976.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2317..2604
                     /locus_tag="U4B62_002882"
     CDS             2317..2604
                     /locus_tag="U4B62_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142977.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          960 a          449 c          544 g          781 t
ORIGIN      
        1 tcttttcatg ttaagtggat tttaaaatgt aaaaatagta tgataagacg agaataacaa
       61 cgtggggtgt ttacatggaa aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag
      121 agaacattcg aatcacacct caacggtacg cggttttaga atatttgatt gaacatcatt
      181 cccatccaac ggctgatgag atttataaag cacttgaaca tcggtttcca aatatgagtg
      241 ttgcgactgt ttacaataac cttcgcttat ttacttcgat tggatttgtt caagaaatga
      301 agtacggcga tgcttctagc cgatttgatt tcagttcaaa gcgtcattat catgtaattt
      361 gtgaaaactg cggtaagatt gttgattttc attacccagg gcttgaagat atagaaatgg
      421 ctgcaagcag actgactgat tttgagatta atgaacatcg gttggaattg tatggactct
      481 gtccggattg taaagagtca gcaaaaaagg cgcaaatgta aaaacagttt ctacaagtgt
      541 tatacctaaa aaaagctagc tgtgtaaaaa aaacgctagc ttttttattt ttttgtcttt
      601 tattcacaat cttagatata aaagtgaata ttattaaaat aagaatcttg tagatattgg
      661 gttcttatgc ttgaaaagga atgaaactac aaacaaacct ttgtgaattc acaagcatta
      721 aactgttgta tccgttttat ttctgttata gtagttttgt cgaaggacaa aatgttaatc
      781 ggagggacca taaatgaaag tagtagtaat tggctgtact catgcaggta ctgcagctgt
      841 aaaaagtatt ttaaaaaatc atccagaagc agaagtgaca gtatatgaaa gaaatgacaa
      901 tatttctttc ttatcttgcg gtatcgcact ttatgtcggc ggagtagtaa aggacccagc
      961 aggcttgttc tactcaaatc ctgaagaatt atcttcatta ggagcaacag ttaaaatgga
     1021 acacgatgta acagacgtcg atacagaaaa taaaacagta acggcaaaag atctgaaaac
     1081 aggagaaaca gaaacggttt cttatgataa attagtgatg acaactggtt catggcctat
     1141 tatcccaccg attagtggaa tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca
     1201 agcaaatgaa attattgcac aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg
     1261 atatatcggt attgaattag tagaagcttt tgtcgaatct ggaaaacaag tgacattgat
     1321 tgatggatta gatcgcatct tgaataaata tctagataaa ccattcacag atatcttaga
     1381 aaaagaattg acagaccgcg gtgttactct tgcgctaggc gaaaatgtta ctgaatttat
     1441 cacagatgaa gaaggaaaag tgaaacaagt agctacacca acagatacat ttgatacaga
     1501 tatggtcatt ttatgtgtgg gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga
     1561 tacactgcca aacggggcaa tcaaagtgaa tgaatacatg cagacaagta atccagacat
     1621 ttttgcagct ggagactctg cagtagtcaa ctacaatcca agtggcacgc aaaactatat
     1681 tcctttggca acaaatgccg ttcgtcaagg tttgcttgtg ggaaataact tgacagatca
     1741 taaaatggcg tatcgtggta ctcaaggaac ttcaggactt tacctatttg gctggacgat
     1801 cggttcgaca ggtgtgacca aagaaagcgc aactttgaac gatctagaag tccaagcgac
     1861 tgtatttgaa gataattatc gtccagagtt catgccaact actgaaaaag taatgatgga
     1921 gctagtctat gaaaaaggaa cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga
     1981 cattacacaa tcagcaaata ccatgtcttt agctgttcaa aataaaatga ctgtagaaga
     2041 tctcgcactt tcagatttct tcttccaacc acattttgac cgcccatgga attacttgaa
     2101 cctacttgca caagcagcgc taggcgaaat cgaaactgca caataaagaa aaagggttgt
     2161 gacacaattt tgtcacgacc ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc
     2221 ttcgaatttt ttgtcacaat aagcagcaaa gataaagctg ttacataaaa gaacaagcag
     2281 ctttatttga ttttgaaaag aaaggtgaga aagaagatga ataaagaata ttttgtccaa
     2341 caagatgcat ttttagaaga ttataaaagt gcttcgaaga aattaacgat caatattgat
     2401 gagttggcag atcaagtact ctctcagttg aatgaaacgc aagaggaata ttacacgatg
     2461 ccagcaaatg acgccaaaga taatgtttct catcgttttc ctttcagtag acgcatctac
     2521 tcagatgaaa atgacggcac attgaataca gagtacacat atgaaggtca gccatatgtg
     2581 ctagaaactg atgatgatct ttaaagacag tttttgaaaa ggccgctcat ccttaagtga
     2641 tgacaaaaaa tcatcacaat taccgatgac tgtctcttta ctatctttta atcagaaaaa
     2701 ggggttatga caagctttgt caaccccttt tttt
//
LOCUS       DASJUF010000199         2643 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJUF010000199 DASJUF010000000
VERSION     DASJUF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.208"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..516
                     /locus_tag="U4B62_002883"
     CDS             172..516
                     /locus_tag="U4B62_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN2142978.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="U4B62_002884"
                     /pseudo
     CDS             517..936
                     /locus_tag="U4B62_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="U4B62_002885"
     CDS             complement(1028..1378)
                     /locus_tag="U4B62_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142979.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2564)
                     /locus_tag="U4B62_002886"
     CDS             complement(1344..2564)
                     /locus_tag="U4B62_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN2142980.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT          985 a          477 c          518 g          663 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 gga
//
LOCUS       DASJUF010000200         2619 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.209, whole genome shotgun sequence.
ACCESSION   DASJUF010000200 DASJUF010000000
VERSION     DASJUF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.209"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..317)
                     /locus_tag="U4B62_002887"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="U4B62_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(391..672)
                     /locus_tag="U4B62_002888"
                     /pseudo
     CDS             complement(391..672)
                     /locus_tag="U4B62_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            1134..2516
                     /locus_tag="U4B62_002889"
                     /pseudo
     CDS             1134..2516
                     /locus_tag="U4B62_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          940 a          456 c          433 g          790 t
ORIGIN      
        1 tttcgtaacc agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc
       61 caacgtccct taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta
      121 tttttcttct tccaaagata atagaggact ttgctgtact cttgtaccca acgataaatc
      181 gtagtatgac aaacatttat tccacgatca tatcacaatt cctgaacttc acgatagctt
      241 agattgtaac gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg
      301 cctttaaaat gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat
      361 agattttttg gaaaactttg caacagaacc gaaatgagta tagtatgaat tagtaatttg
      421 gttttcatct aagtttagct ttgatttact aaaattgata tctcgataca tgttcaatac
      481 atctaataca aatcgagaat cttctttcgg aaatggctca gaaagatcac tccaaatttc
      541 atcatcataa tgatactcgt aaccttctct caatatttcg atttttcttt cataataaga
      601 tctgttttgg tcctcaaatt tagccaataa ctcaaattga tttactagtg acaatctctg
      661 aaaactgtcc attaaatgtt tcttcctatc ttgttcatta acaaaaaatt ataagccctt
      721 acaacgaata ttatatatta aatttaattg taggtgaaca cgaattttat aaatatttat
      781 ggtgttaatg acaaatgata tccgactcct cttcatacgt tctggactat ttgctaatag
      841 attaaaattt cagcatctat ttctgaccat aaataaatga gctgccgaaa agctgccaaa
      901 tcagacttga aaataaagga tcaaaaactt ttaagtttct gaccccgtaa ctcacacttc
      961 aaaatcttac ttaaaaagga gtctgaaaga aaaaaagagt attcaaaatt tttttctaac
     1021 tagctacatc acatctatgc attgacactc aaagaaccag aataagtcat cagaaagcaa
     1081 gggggtgcgg acataatcct ggacgaaaaa ataaacttag gaggaatgtc cgtgtgtttt
     1141 cccacaaaga gcgtgtaagg gcaattcagt tatttttaaa atacgattgc tcttatgctg
     1201 caaccattcg agaattaggg tatccgtcga ttggtgcact acggaaatgg tataaagagt
     1261 atttggaatc cggagagctc catcaggagc accgaaaaaa atcaaagtat tctgaagaac
     1321 aaaaacgaat agcggtgaat cattattttg aatatggtca atgttatgca cgaaccattc
     1381 gcatgcttgg ttatcctaat agagagtcct tgcggcagcg gtgtgaagaa cttgcaccag
     1441 gagctagaaa actccgaaaa agtgcggtaa agttaacaca agatcaaaaa gaagctgtgt
     1501 tgaaaaaatt ctataaaccg caaactaata ggaaaaattt agccgaatct ggaggaatta
     1561 gtagagttac tctatatcaa tggaaaaaca cggttcttgg aaaggatttt ccattgtgta
     1621 tgactttaaa aaatcaggaa aaacaaaaag agctactttt aaaggaagta gaagacttac
     1681 aaaaacaagt acaccaatta cagctcgaaa aagcattact cgaaggagcg gctgaactgc
     1741 taaaaaaaga aaaaggcgtc aatcttcttt gtctatcaaa tcaagaaaaa acgatattga
     1801 ttgatgcctt acgaaatcag ttcaccttga aagagcttct tcaacagctc cagtttccta
     1861 aaagcagcta tttttatcaa aaacaagcac ttgagaaacc agataagtat tacaaagaac
     1921 gtcaaattaa ttatcacgat tttcaatcat aacttctgtg cgtatggtta ccgtagaatt
     1981 caccaagctt taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg tagattgatg
     2041 actgaggaaa atctatttgt taaattttct agacgaaaaa agtattcttc gtatgctggg
     2101 gaaatttctc ctgcacaccc caatttatta aatcggaatt ttaaagcaaa gaagccaaat
     2161 gaaaaatggt taacagatat tactgaattt agaattctcg cagggaaaat ctacttatca
     2221 ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac aaagccaact
     2281 tgcttcactc tatgtctaaa aagagttatt caccagataa ttcagcgtgc gaaggtttct
     2341 ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaagaataca acaatcaatc
     2401 agtttataaa caaattggat gattatattc attggtataa taatcaacga attaagttat
     2461 ctttaggaag gctaagccct cttcaatatc ggaagaagca aagatatatt tgttaaaaag
     2521 gtccaaaaaa aatatccgca cccccttttt ttgtaaaaaa ctattgcaca atacaagtag
     2581 cctacggcta tcgaaatttc cataactttc gagcaagaa
//
LOCUS       DASJUF010000201         2507 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.210, whole genome shotgun sequence.
ACCESSION   DASJUF010000201 DASJUF010000000
VERSION     DASJUF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.210"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(58..648)
                     /locus_tag="U4B62_002890"
     CDS             complement(58..648)
                     /locus_tag="U4B62_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2142981.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            795..2147
                     /gene="brnQ"
                     /locus_tag="U4B62_002891"
     CDS             795..2147
                     /gene="brnQ"
                     /locus_tag="U4B62_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2142982.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          738 a          466 c          471 g          832 t
ORIGIN      
        1 ttttgtgaaa tttcggctta tttcttggag ttaaaagctt ctgtcccagc ctcttttcta
       61 tttccccgga aacaaccgct gataataggc atctgctttt ttataggaaa aaatccaaat
      121 accagataca aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga caccggcaat
      181 caagcataag ttaatcatca tggagataag acttcgacgg tcctttgaca aaactctcac
      241 gacatgggaa tctggtttat ttgctttcat gatttgcctc atcaagatac caaaagaaaa
      301 actccacatc gtgtatacta agacataagc catctcaggc caaaaagctt cagggttacg
      361 cccgacccaa ctagtaacag aaggacataa agtcagccaa aagagcaaaa agatattcga
      421 ccagaccact cggtaattaa ttcgttctgc catctgaaat atacgatgat ggttcaccca
      481 gatcaccgca acaaatataa agctgatggt ataagctaat aatgtatttc ggatgaccca
      541 taaagaatga aagctagatg tattagggat cttgatctct aatacaagaa ctctcaaaac
      601 aatggctacc actccatcag taaatgcttc taatctcgac tttgtcacga tgattcctcc
      661 tcagatgatt cagcgctttt attttatcac ttacgttatt aaataaccgt cgttttatat
      721 aggaaatttt ctgaaaattg ttgcatattt ttcgaaattt tgttactttt aaaaaaaccg
      781 taaaggagtt atgtatgaca aaaaaattat catttaaaga ctatcttttt ataggctcca
      841 tgcttttcgg tctatttttt ggtgctggaa atcttatctt tcccgttcat ttgggtcaag
      901 aagcaggcgc tgctaccttt tgggcgaatc taggtttctt ggttaccggt atcggtcttc
      961 ccttcttagg ggtgattgcc attggtgtat cgaaaagctc aggtgtatat gaattagcaa
     1021 aacggataaa tcaaacatat gcgatggttt ttaccatctt gctttactta gttatcggac
     1081 cgttttttgc tttacctcgc cttgcaacca catctttcga gattggttta gcaccattta
     1141 tcgatcctag cagacagacg ctatatctag caattttcag tattgtattt tttgtgctgg
     1201 cttggtggtt ttcaagaaaa ccgacaaaga tattagatta tgttggaaag ttcctgaatc
     1261 ctgcattttt ggttttatta ggtgctttgc ttctccttgc ttttttgaat ccactaggtt
     1321 ctgtaaatca agcgcctatt cagccaaatt atcaagagca tgcttttttc acagggttta
     1381 ctcaaggata taacacatta gatgcgcttg ctgctttagc atttgggatc atcattgtca
     1441 caacaatcca aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac acgatcaaat
     1501 ctggtgcgat cagcattata ttgatgggaa tcatttatac tttgctcgct tatttaggtg
     1561 ctatgagctt aggtagcttt gctctaagtg aaaatggcgg gatcacgctg gctcagattg
     1621 ctgatcatta tttaggtact tatggcagta tcttattagc atttatcgtt atattagctt
     1681 gcttgaagac cggtattggt ttgattacag cttttgcaga aacattcaca gatttgtttc
     1741 cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat ccttccttgt ttagcggcaa
     1801 acgtaggctt gacgaatatc attcaatttt cgttgcctgt tttgatgttc atctatccat
     1861 tagcaatgac actgatttta ctagtgttag ttggtccttt gtttaagcag cgtacggctg
     1921 tttatcgaat gactacttat tttacattga ttgcttcgat ttttgatggg ttgaacgctt
     1981 gcccagaaac tatcaaacaa acacctattg ttcaaaatct tttatatgtt gcggaaagct
     2041 acttgccgtt tttcaaattg gggatggggt ggatcgtacc agcagttatc ggttttgtta
     2101 tcggactgat ttggagtttc gctaagaaag aagaagtagc agattgataa aagaggttgt
     2161 gaaagagacg ttcagcctta ttgccgatag gctgtctctt gaacactgtt tattgagagg
     2221 tcgtgaaaaa gccgctcagt ttcgagtatt aagaaatatt ttttgaaaat ggcttctcac
     2281 atttttgcat aaatttgact taataccgag aaactggctt ttgaacaccg tttattgagc
     2341 gtctataaac tgacctgagt attagagaaa caaaacggca aagtagctcc atatactttg
     2401 ccgttttgtt ttgaactaat accgaagaga agctgatcaa acaacatttc ttttataata
     2461 tagattttag acatatatat gatataaaaa tagttttcgc aaaaaaa
//
LOCUS       DASJUF010000202         2461 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJUF010000202 DASJUF010000000
VERSION     DASJUF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.211"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..189
                     /locus_tag="U4B62_002892"
     CDS             <1..189
                     /locus_tag="U4B62_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2142983.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="U4B62_002893"
     CDS             complement(1055..1585)
                     /locus_tag="U4B62_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEN2142984.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="U4B62_002894"
     CDS             complement(1582..2199)
                     /locus_tag="U4B62_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2142985.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          810 a          469 c          274 g          908 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 t
//
LOCUS       DASJUF010000203         2461 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJUF010000203 DASJUF010000000
VERSION     DASJUF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.212"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..828
                     /locus_tag="U4B62_002895"
     CDS             <1..828
                     /locus_tag="U4B62_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN2142986.1"
                     /translation="EVIVQTSDVEDFFYTIKKYRDELFDWAETYDKIKNFHKQNSTQK
                     KIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKKADPYSDMMKLNELIQEFIEQ
                     YNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIEQANTID
                     DLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEIDSAAKAE
                     EIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENTEITIQF"
     gene            892..>2461
                     /gene="pglX"
                     /locus_tag="U4B62_002896"
     CDS             892..>2461
                     /gene="pglX"
                     /locus_tag="U4B62_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HEN2142987.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKK"
BASE COUNT          978 a          318 c          465 g          700 t
ORIGIN      
        1 gaagtaattg ttcaaacgtc agatgttgaa gacttttttt atacaatcaa aaaatatcgc
       61 gatgaattat tcgactgggc tgaaacatat gataaaatta aaaatttcca caagcaaaat
      121 agtactcaga aaaaaatctg ggagaatgca caagataaaa ttagattaat cgaaaaaagt
      181 cggcgtcaaa cttctagcca agaaattgat caattgattc atgagatgaa aaccattttg
      241 aaaaaggctg atccttattc agatatgatg aaactaaatg agttgattca agaatttatt
      301 gaacaatata atcaactgtt agagttggaa agtcaggaaa tattaaataa attaacggaa
      361 gaaaaacaag caacatttaa attattagaa ggaaaagatt ttaaggatca atttgtacca
      421 atagttgacc aagaatttca agaactgaaa gcaaaaattg aacaagcgaa tactatagat
      481 gatcttgttt tcttatcatt aaacgtagca gaacagtatt tgatctttaa tcaacgtttc
      541 gataatgaag aaaaccgcag acgtcaagaa gaacaagcac gtaaacaaat tgaagcagca
      601 aacttgattc gtgaaactga aaaagatgaa atagactctg cagcaaaagc tgaagaaata
      661 gcgatagtaa aacctactat tcaacagaaa acagtaccaa tgagaacact taacgttcat
      721 gttaaaacta ttaagtcagc agcagatatt gatgagttaa cagctgaaat taatcaaaaa
      781 ctgaaggaac aacttcaaga aaatacagaa atcacgattc aattctagtg aaaagacagg
      841 cataaaaagg tgcctgtctt ttcaaaggtt acatatagaa gggaaagaaa aatggataag
      901 aaagcgataa agaatttcgc ggtcaatgcc cgtagaaaat taattgatga agtaatgact
      961 aaagcaaaac gtttaggaat tgatgaatca aaaattgaag atgtgaagaa aattgatagc
     1021 aatttacaag aaatcgaaaa atcaggaata agaattcaag gaaaagaaat agcgcaaaga
     1081 aacaaattga ttgcagaact aaaacaacgt gcttctgtca cttcttattc agaagctttt
     1141 caagagttaa tcgaagaggt tgcttatacg tggtttaacc gattgattgc cattcggttt
     1201 atggaggtaa atgattattt acctgacacg tatcgtgtat tatctagtga aataagtggg
     1261 aaaattgagc cagatattat tacagatatt tatgatgccg atatctttga agaattatct
     1321 agtaatgaac agcaacaagt cagaacttgg aaaacagata attcagcaga agcaatggac
     1381 aagctgtatc aattgatttt tataaaaatc tgtaatcaat tgaatgatta cttacctgag
     1441 ctatttgaag aagtagaaga ttatacagaa cttttgttta cagcttctta tattaaaaaa
     1501 gatggtgtaa tcgctgatct tttaagtatt ccagaggatg atttctatgt tcaagctggt
     1561 ggacaggtag agattattgg gtggttgtat cagtactata atagtgagcc acataatcaa
     1621 gtggtgaata tttataaagg gattgtcaaa aaacaagata ttccggcagc aactcaacta
     1681 tttaccacag attgggtcgt tcgttatatg gttgacaata gtttaggaaa gtattggtta
     1741 gatcattatc ccaatagtga attaagaaat aagttgaaat acttaattcc agataaatat
     1801 gcttttgaac aagcaacatc tgtaagtaat ttaaaaccag aagaactaaa aattatcgat
     1861 aacgcaatgg ggtctgggca tattttagtt tacgccttcg atgtattgat ggaaatttat
     1921 cttacgttag gatattctga gcgtgatgct gcacgtgcaa tcgttgaata caatttatat
     1981 ggtcttgaaa tcgataaaag agcgtatcaa ttgtcttatt ttgcaattat gatgaaaggg
     2041 cgtcaatacg atcgtctttt tttgaaaaaa agtctatcgc cgaatttaag agagtttatt
     2101 gattctccta aagtgacaga cgagtatcaa gagcgtatta gtgaattgaa caatgataag
     2161 tatagtgaac gctggaatga atacaaagag ttaatttttg atcaatttga aaatgggaaa
     2221 gaacttggat caatcattaa gttagtcgat aaaaataatc aggcaataac taaagaatac
     2281 ttaggaaaag taagagagta tatatcaaaa ttcaacgaat ttacgaatat ggatattctt
     2341 tatgggatga aggaaataca agaaaaatac ttgcacattc ttgacgtggc tgaaatgcta
     2401 gttgataaat atgtagcggt agtcactaat ccgccttatc ttaataaaat ggataagaaa
     2461 t
//
LOCUS       DASJUF010000204         2419 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.213, whole genome shotgun sequence.
ACCESSION   DASJUF010000204 DASJUF010000000
VERSION     DASJUF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.213"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..1150
                     /locus_tag="U4B62_002897"
                     /pseudo
     CDS             6..1150
                     /locus_tag="U4B62_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="U4B62_002898"
     CDS             1321..1779
                     /locus_tag="U4B62_002898"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN2142988.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="U4B62_002899"
     CDS             1938..2408
                     /locus_tag="U4B62_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN2142989.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          456 g          699 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaa
//
LOCUS       DASJUF010000205         2400 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJUF010000205 DASJUF010000000
VERSION     DASJUF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.214"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="U4B62_002900"
     CDS             207..2069
                     /locus_tag="U4B62_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2142990.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="U4B62_002901"
     CDS             2069..>2400
                     /locus_tag="U4B62_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2142991.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DASJUF010000206         2394 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJUF010000206 DASJUF010000000
VERSION     DASJUF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.215"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(332..922)
                     /locus_tag="U4B62_002902"
     CDS             complement(332..922)
                     /locus_tag="U4B62_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEN2142992.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(1240..2301)
                     /locus_tag="U4B62_002903"
     CDS             complement(1240..2301)
                     /locus_tag="U4B62_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2142993.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
BASE COUNT          799 a          458 c          263 g          874 t
ORIGIN      
        1 aacatacgag tgttatttcg tatcccccag ataatcaatc ctatattttc taaattaata
       61 actgagctac aaatagaaac tagtaaaaac aaaactatca gcatctcatt tttgctagca
      121 tatattcctt ttctgcgtct aaagctaagt gcatttaata acacaaagat attaattata
      181 tctcccaaat ataaaattgc tcttggggcg ttaaaagtat atacaagaac tatggagatt
      241 gcaaaaaatg ccaacgtgaa caatgtccaa tctgctcctg accatctttt tattgaaata
      301 cgcatcgtta ttcaccttct ttcgctaaaa attattctaa aaatactttt gttaaattaa
      361 cataccctat tatttttgcc ggtacacctg caagaattgc accattctct agacaatctt
      421 tgataacaat agctcctgcc gcaacaacta cattgttagc tatttttaca ttaccaataa
      481 tctttgcacc cacgcataca cgtacattat ttccaattac aggacactta ggatcgattc
      541 catcatttcc aatacagtta ttcccatata agtaacaatt ttttcctatt atggcgttac
      601 cattaataac aattccttgt gtatggtaaa atcttacacc ttcatcaaat acattttcac
      661 cacactctat accacatttt cttcccaaac tattcattct tctgcaagac acaatataca
      721 taatcgcata aatgatattt ttctttctct ttgaataata ataagatgta attctcattc
      781 gacgagtata ccgccaagcg ttgtagtccg gatgtgattt cattattttt agtaattttg
      841 tgtgttgtcc tttagattct atatatagat tcttttccat acgaagaacc gcttttaaat
      901 attctttact gctaatttcc attcatatct cctattaatt caatttatac ttaacaattt
      961 tattcggatt tcttgcaaca agagcatcat tcaatgctac tgaatctaca acgatggata
     1021 tgttatcttc atcgttgatt ccaccggata caatattact tataaaaatc gtaacatttt
     1081 ttccaaaaga agggatgtcc gtgatgtata atgcccattt tccgatattt ccagttattt
     1141 aaacctaacg aatttttaca atagtacaag tatcacaatc attaatatat aactggaata
     1201 taaatctcca aaaattcact atagtacagt tttttatgat taaaatctcc tatttctacc
     1261 cattccgctt aaatatgcta atgttttaac cccaataatg gattggttta gtaaaaaata
     1321 gttaaatatt cgttgtttaa ttggattttg tttatatggg tatgagtgaa tacaggcaat
     1381 caactcatcg ttcataagta tcttagacaa ataggagttt aaatcaaatt ctttattaaa
     1441 actagcatat ctaactgcct tttgaataca agtacgacag cgtcccaaca aaagtctcct
     1501 tgttttgttt acaaatatct cattttctaa tatatagctt aaatcgcctt caattttttt
     1561 aactaattca atattacaat cgaactcatt atctctaaaa ttatgtgtca atgaattatt
     1621 attattacat ctataataat agatacaatc tttaataaaa cttgttattt ttgaatgttg
     1681 caaatatgaa atatggaata tgatatcttc agatatataa tccctttctg acggaaactt
     1741 gatattgtgc tgttgaatta tctcagatga atacagcgca tgccaaactg acattggcaa
     1801 tataaaatcg actttataac tcggctcagt gccaatcatt ccaagcaaca ctttttcaat
     1861 tatagcgtta tttgtaaata tcgtactatc atatcttgct ggctttgaaa taatattatt
     1921 atcatcataa tattcataat atccagcata acatgtgtct gcattactcc tttttatacc
     1981 atttaacatt gcttcaatat aattagctgc aacaaaatca tcagaatcaa taaaagcaac
     2041 atattttcct tttacatgtt caagtcctgt atttcttgcc aatcccaagc cagcattatt
     2101 tttatgaata atacgaatcc ttgaatcctt taatgcatag tcttcacata ttttattaga
     2161 tgaatcctta gatccatcat ccacaattat aatatctagg ttcttatatg tttgattaca
     2221 cactgattcc aaacaacttc ctacatactt ttcaacatta tatacaggta tgattacact
     2281 aattagatca ctcgaattca tccttctatc ctttcactca cgttttctca attagtcaat
     2341 gacggcatca acgattttct taaattctct gaaaaaaata gactctgatc ttga
//
LOCUS       DASJUF010000207         2387 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.216, whole genome shotgun sequence.
ACCESSION   DASJUF010000207 DASJUF010000000
VERSION     DASJUF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.216"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(4..249)
                     /locus_tag="U4B62_002904"
     CDS             complement(4..249)
                     /locus_tag="U4B62_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2142994.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="U4B62_002905"
     CDS             complement(266..1117)
                     /locus_tag="U4B62_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2142995.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="U4B62_002906"
     CDS             1283..2287
                     /locus_tag="U4B62_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN2142996.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          762 a          403 c          482 g          740 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtg
//
LOCUS       DASJUF010000208         2375 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJUF010000208 DASJUF010000000
VERSION     DASJUF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.217"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(252..620)
                     /locus_tag="U4B62_002907"
     CDS             complement(252..620)
                     /locus_tag="U4B62_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN2142997.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            770..1252
                     /locus_tag="U4B62_002908"
     CDS             770..1252
                     /locus_tag="U4B62_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN2142998.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1289..2299
                     /locus_tag="U4B62_002909"
     CDS             1289..2299
                     /locus_tag="U4B62_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN2142999.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          834 a          366 c          471 g          704 t
ORIGIN      
        1 ttttctcgta ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt
       61 tactaagtag aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc
      121 cttataaaaa tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat
      181 aggattgaac aaaacctgta taaggtgatg aatagtttcc tcgccagtaa aaggtttatt
      241 cgtataaagc tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa
      301 atcaattgtt caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta
      361 tccgtatata cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt
      421 tccggtgcga tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg
      481 tcagtaaaca taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt
      541 atcttagcaa aaaggtcttt gatcttttgt tgatgttctt tttctttgat ttgattgatg
      601 atcggctgaa atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca
      661 gaaaaacaat agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata
      721 tttatatgat aagatgtctc aaataataaa atagaaaaga gacataaaaa tgattgactt
      781 ggcaaagaaa ttcttacacg aaaatatata ttttgagttg tatcatcatc gagccattta
      841 tacaaatgaa gatgctttgg taataaagaa agaacaagga tttacaggta cagagaccaa
      901 aagcttattc ttaaaagata aaaaagataa taactacgta tatctgactt ttactacgaa
      961 acaaacggat ttcaaagcat tagggaagat cgtcggtaaa aggctttcta ttgtttcatc
     1021 agaacgcatg gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt ttggttatga
     1081 aaaacagatt ccgattatta ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc
     1141 accaggtaga ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca aaatcatcca
     1201 gctacttgag atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa
     1261 aatagtgcat acttggaggt gaagcaacat gataagttat ccattattag aaagaaatgc
     1321 aacgattggg gttactgcac catcctcagg cgtatcagag gaacaccatt cgttactcga
     1381 atcagcttta aagagaatgg aagaaaaatc tttccaagta gttactggaa agacgctttg
     1441 gacgcaagat aaggaaaaat caggtccagc aagaaaacga gcagatgaat tcaatcaaat
     1501 gatgcaagat gaaaatatcc aaatgatctt tccgacttgg ggaggagaat tattgattga
     1561 gattctcgat tttgtcgatt ttgatgctct acaaccaaaa tggatagtag gttattcaga
     1621 tattagtacg ttgctacttg ctgtcacatt aaaaacaggg atagccactg cacatggaac
     1681 taaccttatt gatttaagag gagagtactt ggatgaaacg acagcaatgt gggaaagtgt
     1741 actgaaaaca agagaaggag aatcgattct acagcaatct tctgaaaagt atcaaaaagt
     1801 gtggccccat gagcattcat catcttctgt tttccatctc actgaaccaa caaattggca
     1861 atcagtcagc ggagatgcag taaggatgaa gggacgatta ttaggtggtt gtattgatac
     1921 gatcaggcat ttaatcggta ctccttatgg agatgtccaa tcttttaaaa gaaagtataa
     1981 tgatggaaag ccgatcttgt ggtttctaga aaactgtgag ctatcgtcag ttgatttaag
     2041 aagatcactt gttcagatga aactggcagg ctggtttgat ggcagctcgg ggatcatgtt
     2101 cggtagaagt gcggcagagg atttgttaga ttataaaatg aaggatgtat acgaagatct
     2161 gtcaaaggaa ttaaaactac cgatcttgta tgatattgat tgtggtcatg taccgccaca
     2221 actgacattg attaatggtg cctatgcaga agtagcggta gaagatagaa aagcgactgt
     2281 tttgcaaacg tttatttaaa aaattttttc ctgccttgga gtctatcaaa gaccaataaa
     2341 tctaaataga aaagaaagtg aaagaagctg acaaa
//
LOCUS       DASJUF010000209         2350 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.218, whole genome shotgun sequence.
ACCESSION   DASJUF010000209 DASJUF010000000
VERSION     DASJUF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.218"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..2203
                     /locus_tag="U4B62_002910"
     CDS             50..2203
                     /locus_tag="U4B62_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN2143000.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          806 a          416 c          527 g          601 t
ORIGIN      
        1 gtttttgggt acaatgaagc atgaatgtaa ataggcggaa ggataggaaa tgatgaaaga
       61 ttttatgaaa aaaatattaa atacttcttg gttagaaaaa ataagaaaag gtgcacaaga
      121 acctaagaac aaaacgtttc gccggtccca tgttcctttt cgcttaaatt ttcttttttt
      181 cattatattt acgttatttg ttgcgttgat tgctagattg ggttatttgc agatcgtgaa
      241 cggcgaagag atggaggcgc gagtgaagtc tacttctacg attacggtcc aagaaagtac
      301 tcctcgcgga atgatctacg actccacagg aaaagcttta gttacgaata aagcgaatca
      361 ggccatcaca tatacgagag gaacacaagt tacggctaaa gatttgcttg aaatcgcaac
      421 gaagctgaac tcgctgatcg atgttccggt agatgaaaat ctgacagata gagataaaaa
      481 agatttttgg ctggctgatc ccaaacattt gaaagaagcc acaaaaagac tttcggcaaa
      541 agaaaagcaa ttagggaaca gcgaacaata tagtgcgatg gtcaataaag tcacagctga
      601 tgagattaat ttaaacgagg agcaattaaa aattgctacc atctttaaac ggatgaatag
      661 tgcatatgaa ttaaacactg tctatatcaa aaactctggt gttactgatc aagagttagc
      721 tgttgtggca gaacgcgctt ctgaacttcc aggagtttcg actggcacag actggacacg
      781 agaatataat gcagcaagtt cactgaaaag tattcttggc agtgtgacga ctgaaaagca
      841 aggacttcca gcagatgaag cagagaaata cctagcaaaa ggttattctc ggaatgaccg
      901 tgtaggacaa agttatttgg aaaaacaata tgaagatgtc ctgcaaggaa ccaaaaccca
      961 atatgaagtt tcccttgata atgaaggaaa tgtttcgaat caaaaagaaa tcttctctgg
     1021 ggaaaaaggt tctaatttaa tgttatctat gaatgcagaa ttccaatcaa aagtagaaga
     1081 aattttgaaa aggaattatc aaacattgat caacaacggc aaagcacagt attcgcctgg
     1141 tgcttatgca gtggctatga atcctcagac aggagaagtg ctagcaatga ctggtttcag
     1201 tcatgaacaa ggttccaaag aaatcaccga gaatgcttta ggcacgatta ctagcgcctt
     1261 tgatccaggg tctgtagtca aggctggtac tttgacagca ggctgggcaa gtaacgcgat
     1321 cagcggaaat caagttttga ttgatgagcc gattcgtctg caaggtgctt cagaaaaaag
     1381 ctctgtcttt aatcgaagcg gacaagttgc tcttgatgca gtcaaagcat cggaattgtc
     1441 ttctaatacg tatatgatca aagtggcgtt aaagatgtta ggactagact atacacctgg
     1501 aatgggacta cctagtttag atgaagaagc taaagcttat cagcaattga gagactcttt
     1561 caaagagttt ggtcttggta caacgactgg gatcgatttg ccgaatgaat ctcctgggat
     1621 ctcacgttct gttgattata tgaagaagtt caatgcggat aatggcaaag aatggtatgc
     1681 tccaggtaac tttaccgatt tggcatttgg acagtttgac acatatacac caatccaatt
     1741 agctcaatat gcttcaacag tcgctaatgg cgggaaacga gtacagccgc acctagtcaa
     1801 agcgatctat ggcaatgacg aaaatggaaa cttgggtgaa gtgaagaaag aaattggcac
     1861 aacagtagaa aatacggtca atatttctgc agaaaatatg gcgatcctcc gtgaaggatt
     1921 ccaccaagtt gtccatggta ccgatcctta cacaacagcc aaaccattag ccagtgcgaa
     1981 aatggacctt tccgcaaaaa cagggactgc agaaacggtt gcggaaggtc atccagatat
     2041 tgcgacagtc aatagtaata tcgtggcata cggaccaaca gacaatccgg aaattgccat
     2101 cagtgttgtc ttgccgaact tattagacga acaagaccac ataaacttga cgattgctaa
     2161 agaaataatg gacgcgtatt atgatatgtt catggcaaaa taaaaaagag gtcgtgaaaa
     2221 agccgcttag tttcgagtat gaagaaaaat tttgtgaaaa tagcttctca catttttgct
     2281 taaatttgac ttcataccga gaaactggct tttgaacacc gtttactcgg caaaaaaggt
     2341 tgtgacaaaa
//
LOCUS       DASJUF010000210         2315 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJUF010000210 DASJUF010000000
VERSION     DASJUF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.219"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            149..1762
                     /locus_tag="U4B62_002911"
     CDS             149..1762
                     /locus_tag="U4B62_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2143001.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          730 a          408 c          448 g          729 t
ORIGIN      
        1 taaaatagaa aaaaattttt aggttttatc cacaaattat ttctctatat tttctgtcta
       61 ttcaaaataa acaataattt tccggcacaa ttatttgctg ttttttacca taacatagta
      121 cgatcaacgt agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg
      181 cggtccttca tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta
      241 tgaacgcgga gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt
      301 gatcactgac cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa
      361 catcgatgtc tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac
      421 agttaaagac ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc
      481 ttctggtgct agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt
      541 tgtcctccgt agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc
      601 acaacatgtc gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa
      661 gcatcgcggc ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga
      721 tccagaaatt gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt
      781 agaaacaaga gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc
      841 cgtcctttct gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt
      901 gaaagcaacc ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca
      961 aaccaatctg ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac
     1021 tggtcagccg atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc
     1081 agatatttta aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa
     1141 aatctttgat catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg
     1201 cataacaaat tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga
     1261 agctacaaga ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg
     1321 acaagcattt ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac
     1381 cggaaacttg acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc
     1441 tactacccgt gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat
     1501 cgtagaaacg atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt
     1561 agatatacgt gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc
     1621 aatgaatgag ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac
     1681 tttcagaaga ggattgaata cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa
     1741 agcgattttg attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta
     1801 tttttacttg aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag
     1861 aatagaagat aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt
     1921 taagaaagtt taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac
     1981 atgttgtgac tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta
     2041 atattacgag gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga
     2101 atattttttc ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg
     2161 cttgcgttgt gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata
     2221 tttttcgcca tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta
     2281 aataatttaa tttttttgtt cctatttatt taaca
//
LOCUS       DASJUF010000211         2278 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJUF010000211 DASJUF010000000
VERSION     DASJUF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.220"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(594..959)
                     /locus_tag="U4B62_002912"
     CDS             complement(594..959)
                     /locus_tag="U4B62_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143002.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1035..1280)
                     /locus_tag="U4B62_002913"
     CDS             complement(1035..1280)
                     /locus_tag="U4B62_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2143003.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1350..1604)
                     /locus_tag="U4B62_002914"
     CDS             complement(1350..1604)
                     /locus_tag="U4B62_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143004.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1768..2091)
                     /locus_tag="U4B62_002915"
     CDS             complement(1768..2091)
                     /locus_tag="U4B62_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143005.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          862 a          372 c          392 g          652 t
ORIGIN      
        1 taagcttttt taaagaatat ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa
       61 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
      121 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      181 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      241 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      301 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      361 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      421 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
      481 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
      541 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
      601 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
      661 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
      721 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
      781 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
      841 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
      901 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
      961 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
     1021 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1081 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1141 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1201 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1261 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
     1321 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
     1381 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
     1441 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
     1501 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
     1561 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
     1621 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
     1681 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
     1741 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
     1801 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
     1861 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
     1921 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
     1981 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
     2041 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
     2101 ataaaagaat aacatttttt ctaaaataag caaataaaaa ttaaagtaac aaaatagtta
     2161 tgacacaagt aaaagaaata agaataacaa tattatttta cttatatttt gaaacttgtg
     2221 agagtttgtc attcgtgcta tagttgaaaa gtaacaaaaa taaattttat ttatgatg
//
LOCUS       DASJUF010000212         2271 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.221, whole genome shotgun sequence.
ACCESSION   DASJUF010000212 DASJUF010000000
VERSION     DASJUF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.221"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            192..446
                     /locus_tag="U4B62_002916"
     CDS             192..446
                     /locus_tag="U4B62_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2143006.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="U4B62_002917"
     CDS             447..764
                     /locus_tag="U4B62_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2143007.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..2258
                     /locus_tag="U4B62_002918"
     CDS             1323..2258
                     /locus_tag="U4B62_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143008.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          430 g          711 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatgaatatt acaagtaaca aaacgtcacg tgtgctattg gcgttgatgg
     1801 gttacggaat tctttataga gatgaccaaa atggccagat tctaataaat cgggctgggc
     1861 gttcattatt aatgaaatat agaaaattta taccggaaga ttttcaaaac ttacagttag
     1921 aaaacatgcg tttagaagat ttatttagat tagcagaaga tgataagtat agatttgaag
     1981 ttttgaaaat tcaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
     2041 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
     2101 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
     2161 taacagatat ctatgtagaa gatgaagata taatcaaata tatgaagcct gtgataaaac
     2221 ttgttaataa aaataaagga acccgtggtg ctagttaatt ttttcctaga g
//
LOCUS       DASJUF010000213         2256 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJUF010000213 DASJUF010000000
VERSION     DASJUF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.222"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="U4B62_002919"
     CDS             complement(<1..914)
                     /locus_tag="U4B62_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN2143009.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="U4B62_002920"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="U4B62_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN2143010.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="U4B62_002921"
     CDS             2006..2176
                     /locus_tag="U4B62_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143011.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          759 a          390 c          427 g          680 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attctt
//
LOCUS       DASJUF010000214         2205 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJUF010000214 DASJUF010000000
VERSION     DASJUF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.223"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..161
                     /locus_tag="U4B62_002922"
     CDS             <1..161
                     /locus_tag="U4B62_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2143012.1"
                     /translation="LPLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQGATNLKHRLY
                     KKIQKMLV"
     gene            255..446
                     /locus_tag="U4B62_002923"
     CDS             255..446
                     /locus_tag="U4B62_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2143013.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            960..1292
                     /locus_tag="U4B62_002924"
     CDS             960..1292
                     /locus_tag="U4B62_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2143014.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1588..1743
                     /locus_tag="U4B62_002925"
     CDS             1588..1743
                     /locus_tag="U4B62_002925"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN2143015.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2019..>2205
                     /locus_tag="U4B62_002926"
     CDS             2019..>2205
                     /locus_tag="U4B62_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2143016.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHRHYQNEQTIIK"
BASE COUNT          791 a          371 c          377 g          666 t
ORIGIN      
        1 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
       61 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
      121 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
      181 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
      241 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
      301 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgga
      361 gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta
      421 tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag
      481 atagtttcaa atttcgaaca ataaatttga aactatcttc tttataatta taaagtatcg
      541 attttacagc gttctataat gattcataga atctaaaacg aatgatttta attatcaacg
      601 ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc ctaaaattac
      661 tttatttacg tatgaagtgg gcaggaggtc gaatccagaa ctgggctatg gtacttcaac
      721 gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg acggtagatt
      781 tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa gagaattttg
      841 gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc tgatactaag
      901 ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa tgaactctat
      961 tgagaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc cttgagaaaa
     1021 aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca atttcccgat
     1081 gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga aaaagattac
     1141 ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa gaggaagacg
     1201 agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa aactttacga
     1261 tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg
     1321 cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat
     1381 ggtaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgatg
     1441 gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta
     1501 gcaactcgta ttctacgtta ctatatggta gattcacaca tgaaaaaaga gattgtcgtc
     1561 cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc
     1621 cagcatcggt cttttattta tcaagagctg ttgaacgacc accacatcac acattcagtg
     1681 agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa
     1741 tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa
     1801 gattatttag aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct
     1861 tgacaatttg aagcgaagaa aataagggat ctataatatc tagtgttggt gacgccttct
     1921 gttgccaata ctttttcttt tttctaataa acgcaaacag acaccttaaa atcctttaga
     1981 ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat gtcctattca gagtttacca
     2041 aagagttact agatattcta gacctaaatc ttatctttca tgaagatgcc tttcgcaaag
     2101 agcgaatcaa tgacgaaaca tgttttgttt ttgatggcac attgacctat caaccggagg
     2161 aatgctttca tcgccactat caaaatgaac aaacgatcat taaat
//
LOCUS       DASJUF010000215         2134 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJUF010000215 DASJUF010000000
VERSION     DASJUF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.224"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..>2134
                     /gene="acm"
                     /locus_tag="U4B62_002927"
     CDS             19..>2134
                     /gene="acm"
                     /locus_tag="U4B62_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN2143017.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          841 a          343 c          416 g          534 t
ORIGIN      
        1 ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt
       61 attactaata taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc
      121 agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata
      181 aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg
      241 atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta
      301 atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg
      361 tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag
      421 gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt
      481 gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt
      541 tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat
      601 attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt
      661 ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag
      721 gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt
      781 aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt
      841 aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag
      901 aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc
      961 aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa
     1021 ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa
     1081 ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat
     1141 gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa
     1201 gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata
     1261 aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac
     1321 atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa
     1381 ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta
     1441 gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac
     1501 gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt
     1561 tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc
     1621 tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc
     1681 acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat
     1741 gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat
     1801 actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa
     1861 aacagttata caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg
     1921 aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg
     1981 caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga
     2041 gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa
     2101 aaatctagtt ttgctgtaat cattggttta ttga
//
LOCUS       DASJUF010000216         2133 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJUF010000216 DASJUF010000000
VERSION     DASJUF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.225"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(96..1022)
                     /gene="cysK"
                     /locus_tag="U4B62_002928"
     CDS             complement(96..1022)
                     /gene="cysK"
                     /locus_tag="U4B62_002928"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN2143018.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1162..2049)
                     /locus_tag="U4B62_002929"
     CDS             complement(1162..2049)
                     /locus_tag="U4B62_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2143019.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          590 a          476 c          356 g          711 t
ORIGIN      
        1 ctattcaaat aatttttctc atcataaatt tttgtgtgtt cttttcatct ttaaaagttt
       61 tttacataaa cctatctgga gaaaaaccaa gtgccttata tctcttggta aagggctgta
      121 gataaataac gttcaccatt gtcgggaacc acagctaata ccttcttgcc ttttcctaat
      181 tcttttgcga cttttagcgc ggcagcaatt gcagcgccag atgagatgcc tactaaaatc
      241 ccttcttttc gcccaacttc tctagctgtt tccatcgctt cgtctccact aatagaaagg
      301 actttatcat acacagaagt atccagtgtt ttaggaacaa aacctgtacc gattccttgg
      361 attttgtgag gtcccgcttc tttcccttct aaaatagcag attctgctgg ttctaccccg
      421 attaattcga ttttcggata cacacgtttt aattctcggc cagcgccagt gatcgttcct
      481 cctgtaccga tacctgcaac aaacgcgtcc aagccattta ctccaaatgc ttgatgtatc
      541 tcaggaccag tcgtcttctc atgcacaaga ggatttgctt cattttcaaa ttgtaatggt
      601 aaaaagtagc cgttttcttt tgccaaacgt tctgcttctc tgattgatcc agaaatacca
      661 tccgcaccag gagtcaaaat caactctgcg ccgtaccctt tcatcaataa acgtctttcg
      721 atactcatcg tctcaggcat cacgatgatt actttatatc cttttgcaac tcctaccatc
      781 gacaatccga ttcctgtatt tccagaagta ggttcaatga tagtatcacc tggtttcagt
      841 agaccatcgt gttctgcttt ttctatcata cttaaagcaa tacgatcttt tacacttcct
      901 cctggattaa aaaactcaag ctttacaaaa acatctgctg aatcttctgg tacgatttta
      961 tttaatttga cgattggcgt tttgccaatc aattcggtga ctgagttata gatagctgtc
     1021 atcttcaatt cctccttgtc tttgctcttc ttacttatca taatacaatc tgttcattta
     1081 tggcgagtga ttcgtctttg actagcaaaa aaaagacaaa gatataccat atatggtaat
     1141 ttctttgcct ttttatctaa atcaaacgtt tagttcttga ccaactaata aagaataact
     1201 ggacatagac atgccatttt tcgccattag atcatcaaca gacatgccaa atttttgtgc
     1261 aatatcccat agcgtatcac ctgacacgac tgtatatgtc tgaccattat ttgtatttac
     1321 atctgttgtt gaagatgatg acgttgaagt cgtcgtagta gtcgttgttg ttgtacttgt
     1381 ggttgttgta gccgaacctg ttgatggcgt atcaaaacgg gttaaattat aagtattaat
     1441 caatgagttc aatttggccc cataatttgg atcggtagca taacgacctg tcaagtacgc
     1501 tgttgcatca taaaaacttg ttgtattgct tttccacacg ccagcataat gataatcgcc
     1561 cgtggaaaaa ctggttgaac gaagaacatt cgcatggtct tggaaggatt cccaataaga
     1621 agaatatttt cggaatggct cattcatcgt tacccattgt ccgtttacat attctttggt
     1681 tgccatataa acgacctgtc cgttatagct tcctttcaca ccgaatagat tataatttgg
     1741 cgcactagct aatccggaag tgccatatgc actttccaac aacgcttgag cgatcatgac
     1801 tgaagcatac aagtcattac ttgcagcaac ttctgacgca gaccagccga tttgatcgat
     1861 aaacgcttga ggatcagcga ctgtttgaga tccttgttca tctgcatgaa catgttgcgg
     1921 taccgctgta accatcatcg gcccaactaa aagactcgtt ccaattaaag gagcaatcgc
     1981 ttttttattg atcaccattt ttcttttagg tctatggtgc cgggcactgc gggtcttcag
     2041 ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc tcttcttaaa
     2101 acaaacttaa ttaattaaat tatactttta att
//
LOCUS       DASJUF010000217         2040 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.226, whole genome shotgun sequence.
ACCESSION   DASJUF010000217 DASJUF010000000
VERSION     DASJUF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.226"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="U4B62_002930"
     CDS             200..742
                     /locus_tag="U4B62_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143020.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="U4B62_002931"
     CDS             898..1347
                     /locus_tag="U4B62_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN2143021.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2040)
                     /locus_tag="U4B62_002932"
     CDS             complement(1377..>2040)
                     /locus_tag="U4B62_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEN2143022.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          733 a          322 c          391 g          594 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggcgc
//
LOCUS       DASJUF010000218         2036 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJUF010000218 DASJUF010000000
VERSION     DASJUF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.227"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..424)
                     /locus_tag="U4B62_002933"
     CDS             complement(<1..424)
                     /locus_tag="U4B62_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN2143023.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="U4B62_002934"
     CDS             complement(533..1564)
                     /locus_tag="U4B62_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2143024.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          627 a          331 c          350 g          728 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgtt tgctta
//
LOCUS       DASJUF010000219         2019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJUF010000219 DASJUF010000000
VERSION     DASJUF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.228"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..570
                     /locus_tag="U4B62_002935"
     CDS             100..570
                     /locus_tag="U4B62_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN2143025.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="U4B62_002936"
     CDS             792..1895
                     /locus_tag="U4B62_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2143026.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DASJUF010000220         2003 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.229, whole genome shotgun sequence.
ACCESSION   DASJUF010000220 DASJUF010000000
VERSION     DASJUF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.229"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="U4B62_002937"
     CDS             <1..378
                     /locus_tag="U4B62_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN2143027.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U4B62_002938"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U4B62_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN2143028.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="U4B62_002939"
     CDS             1859..>2003
                     /locus_tag="U4B62_002939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143029.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DASJUF010000221         1979 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.230, whole genome shotgun sequence.
ACCESSION   DASJUF010000221 DASJUF010000000
VERSION     DASJUF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.230"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(325..528)
                     /locus_tag="U4B62_002940"
     CDS             complement(325..528)
                     /locus_tag="U4B62_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143030.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="U4B62_002941"
     CDS             complement(728..1744)
                     /locus_tag="U4B62_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2143031.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DASJUF010000222         1974 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJUF010000222 DASJUF010000000
VERSION     DASJUF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.231"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            14..1141
                     /locus_tag="U4B62_002942"
     CDS             14..1141
                     /locus_tag="U4B62_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2143032.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..>1974)
                     /locus_tag="U4B62_002943"
     CDS             complement(1359..>1974)
                     /locus_tag="U4B62_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2143033.1"
                     /translation="RPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVRSE
                     ELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTW
                     KKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIE
                     HNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          675 a          353 c          345 g          601 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgc
//
LOCUS       DASJUF010000223         1843 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJUF010000223 DASJUF010000000
VERSION     DASJUF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.232"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..735)
                     /locus_tag="U4B62_002944"
     CDS             complement(34..735)
                     /locus_tag="U4B62_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2143034.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(751..1191)
                     /locus_tag="U4B62_002945"
     CDS             complement(751..1191)
                     /locus_tag="U4B62_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2143035.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1191..1766)
                     /locus_tag="U4B62_002946"
     CDS             complement(1191..1766)
                     /locus_tag="U4B62_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN2143036.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          518 a          400 c          285 g          640 t
ORIGIN      
        1 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
       61 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
      121 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
      181 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
      241 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
      301 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
      361 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
      421 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
      481 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
      541 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
      601 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
      661 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
      721 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
      781 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
      841 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
      901 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
      961 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     1021 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     1081 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     1141 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     1201 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     1261 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     1321 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     1381 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     1441 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     1501 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     1561 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     1621 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     1681 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     1741 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     1801 attctacacg tgttttttta aaataaaaga ctagattttg tag
//
LOCUS       DASJUF010000224         1731 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJUF010000224 DASJUF010000000
VERSION     DASJUF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.233"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..156
                     /locus_tag="U4B62_002947"
     CDS             <1..156
                     /locus_tag="U4B62_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2143037.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="U4B62_002948"
     CDS             169..1077
                     /locus_tag="U4B62_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN2143038.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1697
                     /locus_tag="U4B62_002949"
     CDS             1077..1697
                     /locus_tag="U4B62_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143039.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          613 a          251 c          395 g          472 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaga atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta
      361 gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc
      421 atgcagtgtt tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg
      661 agacaagaac aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg
      721 gcatggtttg aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta
      781 gccgaactaa cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgtttgctgg cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt
      901 gaatacgggg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat
      961 gaagtcgaaa aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca
     1021 accgttgatg atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg
     1081 ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc
     1141 caatcacaca tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag
     1201 ttttatctgt taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag
     1261 agaatgttct cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag
     1321 aaatgtatca aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta
     1381 tcgaccgttt gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag
     1441 tctctgcagt cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt
     1501 cactgaaaaa ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt
     1561 ctacaggtgg tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag
     1621 aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa
     1681 aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc a
//
LOCUS       DASJUF010000225         1726 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJUF010000225 DASJUF010000000
VERSION     DASJUF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.234"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(100..1119)
                     /locus_tag="U4B62_002950"
     CDS             complement(100..1119)
                     /locus_tag="U4B62_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN2143040.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1116..1340)
                     /locus_tag="U4B62_002951"
     CDS             complement(1116..1340)
                     /locus_tag="U4B62_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2143041.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1337..1630)
                     /locus_tag="U4B62_002952"
     CDS             complement(1337..1630)
                     /locus_tag="U4B62_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143042.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          484 a          354 c          291 g          597 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcatt tgtttcctcc ttcttcgctt
     1141 tcagccgaga acattttgta ggttcgattt gatacaccca acacactccc taaaaacgcg
     1201 ccaaaaccag taatgatgac aacacagata tctgtgtact gccaattgag cgctttacca
     1261 actaacccca cgaaagtagc tagtgcggga ataattacca gtgcgaacca ttttagtact
     1321 tcgaacgttt tattattcat tttcttctct ccctaaacaa agttttgatt tgttgcgtgt
     1381 gttccaccaa tttttctgta tgtgtatcta atctttcatc gtgtttcttt agttcttcat
     1441 gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt agtcagcaaa tctaaattgt
     1501 ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga tgcagcaatc ggtctaatta
     1561 ctaataaaat caaagaaacg atagcggtta ttgatcctgc tatcattccc cattccccta
     1621 aattaatcat gtgacaactc ctttacctta aataaaaacg catcaattaa gatgcgctct
     1681 tatctttatt aatgatttta tctgcttctt cgtctgtaat gcataa
//
LOCUS       DASJUF010000226         1658 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJUF010000226 DASJUF010000000
VERSION     DASJUF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.235"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1412
                     /locus_tag="U4B62_002953"
     CDS             <1..1412
                     /locus_tag="U4B62_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2143043.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
BASE COUNT          658 a          251 c          355 g          394 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaat
//
LOCUS       DASJUF010000227         1647 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJUF010000227 DASJUF010000000
VERSION     DASJUF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.237"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..91)
                     /locus_tag="U4B62_002954"
     tRNA            complement(20..91)
                     /locus_tag="U4B62_002954"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="U4B62_002955"
     tRNA            complement(113..200)
                     /locus_tag="U4B62_002955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="U4B62_002956"
     tRNA            complement(225..298)
                     /locus_tag="U4B62_002956"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="U4B62_002957"
     tRNA            complement(312..382)
                     /locus_tag="U4B62_002957"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="U4B62_002958"
     tRNA            complement(384..459)
                     /locus_tag="U4B62_002958"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="U4B62_002959"
     tRNA            complement(478..551)
                     /locus_tag="U4B62_002959"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="U4B62_002960"
     tRNA            complement(564..653)
                     /locus_tag="U4B62_002960"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="U4B62_002961"
     tRNA            complement(677..750)
                     /locus_tag="U4B62_002961"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="U4B62_002962"
     tRNA            complement(764..837)
                     /locus_tag="U4B62_002962"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="U4B62_002963"
     tRNA            complement(865..937)
                     /locus_tag="U4B62_002963"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="U4B62_002964"
     tRNA            complement(957..1030)
                     /locus_tag="U4B62_002964"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="U4B62_002965"
     tRNA            complement(1040..1113)
                     /locus_tag="U4B62_002965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="U4B62_002966"
     tRNA            complement(1124..1209)
                     /locus_tag="U4B62_002966"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="U4B62_002967"
     tRNA            complement(1226..1297)
                     /locus_tag="U4B62_002967"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="U4B62_002968"
     tRNA            complement(1309..1381)
                     /locus_tag="U4B62_002968"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="U4B62_002969"
     tRNA            complement(1399..1480)
                     /locus_tag="U4B62_002969"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="U4B62_002970"
     tRNA            complement(1488..1560)
                     /locus_tag="U4B62_002970"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="U4B62_002971"
     tRNA            complement(1564..1636)
                     /locus_tag="U4B62_002971"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DASJUF010000228         1632 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.238, whole genome shotgun sequence.
ACCESSION   DASJUF010000228 DASJUF010000000
VERSION     DASJUF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.238"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            116..1582
                     /locus_tag="U4B62_002972"
     CDS             116..1582
                     /locus_tag="U4B62_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN2143044.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          455 a          294 c          336 g          547 t
ORIGIN      
        1 aattacttgc ttttatagaa aattagttta tatttcttaa ataagttttt tagttaaatt
       61 tttcggtaaa gatgtataat attttctgaa aatttaaaga attggggtat agattatgga
      121 aaaacagcaa gacaagggct tcttcgggca gccgaaggga ttgtcaactt tgttcttcac
      181 tgagatgtgg gaacgattta gttattacgg tatgcgtgca ttacttatct attatatgta
      241 cgatacagtt gccaacgggg gacttggatt accacgagca acagcattag ctatcatgtc
      301 gatctacgga tctttgatct acatgtctag tatcgtcggc ggatgggtat ctgaccgagt
      361 gttaggagca aaacgcactg tattcttcgg tggagttttc attatgcttg gccacatcgt
      421 cttggcttta ccatttggtg taagtacatt gtttgtttcg atggcattga ttattatcgg
      481 aaccgccttt ttgaaaccaa atgtttctgg catggtcgga catctttatt caaagactga
      541 cttgcgaaga gatgcaggat tttcgatttt ttacatgggt gtcaacttag gggcattatt
      601 agcaccacta atcgttggta caatcggtca agaatataac tatcatttag ggttctcttt
      661 agctgctatt gggatgttct ttggattatt acaatacatc atccaaggac gcaagacatt
      721 agacggagtg ggtacaacac cgccgaatcc tttgacaact gaagaacgta aaggattcat
      781 ccgtaacttg atcatcgcac ttgttgttat cgctgcagta ttcggcgggg cacaactaac
      841 tggtcatctg acaattgact tttttgtcaa tacaatcagt gtattgggga ttatcttacc
      901 gttgtattac tttatcaaaa tgctgacttc aaaagatgta actgctgaag aaaaaccaag
      961 agtatgggct tatatcccat tattccttgc agcaatcatt ttctggtcat tacaagaaca
     1021 aggttcatct atcttagctt tatttgctag tgaacgtaca caatctacgc tatttggtgt
     1081 aacgatccaa gcaagctggt atcaatcatt gaatccaatt ttcatcttta ttctgacacc
     1141 agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa ccatctacag tagtgaaatt
     1201 ctctcttgga cttttatttg caggattatc gtacttgcta ttgatgttgc cgggtatgct
     1261 ttatggtaca agtggaaaag taagtccatt gtggttgttg ggaagtttct tcatcgtggt
     1321 tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg acaacaaaat tagcaccacg
     1381 agcatttgaa tcacaaacaa tcgctatttg gttcttagct gatgcttctt cacaagcaat
     1441 caatgcacaa attgcacgct tctacacacc gggaacagaa tctgcttact tcggtattat
     1501 cggtgcagta gcaatcgtcg gtggtatctt gctatttact gtgaaagaac caatcaaaaa
     1561 actaatgggc tctattcatt aatcaatgaa cataaaaaca ggaattgctt atttgcagca
     1621 gttcctgttt tt
//
LOCUS       DASJUF010000229         1610 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.239, whole genome shotgun sequence.
ACCESSION   DASJUF010000229 DASJUF010000000
VERSION     DASJUF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.239"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(111..278)
                     /locus_tag="U4B62_002973"
                     /pseudo
     CDS             complement(111..278)
                     /locus_tag="U4B62_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="U4B62_002974"
     CDS             complement(544..738)
                     /locus_tag="U4B62_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143045.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(838..924)
                     /locus_tag="U4B62_002975"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="U4B62_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          570 a          241 c          214 g          585 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta
//
LOCUS       DASJUF010000230         1595 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJUF010000230 DASJUF010000000
VERSION     DASJUF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.240"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            66..662
                     /locus_tag="U4B62_002976"
     CDS             66..662
                     /locus_tag="U4B62_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2143046.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(720..1202)
                     /locus_tag="U4B62_002977"
     CDS             complement(720..1202)
                     /locus_tag="U4B62_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143047.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1308..1505)
                     /locus_tag="U4B62_002978"
     CDS             complement(1308..1505)
                     /locus_tag="U4B62_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143048.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          538 a          331 c          248 g          478 t
ORIGIN      
        1 tgctatactt catttcgtaa tcaattaaaa aaatgcaaaa cgcaaatcaa aggagaaatt
       61 ttattatgac aacttttact gacactttga aaaaccgccg ttcaatctat catctaggac
      121 gtaatgtttc actttctaat gaagaattaa caactttgat caaagaagca attaaagaaa
      181 gcccaactgc tttcaatgct caatcaacac gcgcagttat ccttttcgga gatgcacatg
      241 aaaaactttg ggaaatcact gaagaagcac ttcgcccatt gactccagca gaagctttcc
      301 caaatacaca aaacaaatta gctggtttca aaaatggtta tggtacagta ttattcttca
      361 aagatactga cgtggtaaaa ggtctacaag aacaatttga attatatgca gacaacttcc
      421 cagattggtc agaacaatca aacggaatcg caacagctaa tacttgggta gcattagttg
      481 ataaaggatt gggcgcaaac ttgcaacatt acaacccagt tattgacgaa gcagttgcaa
      541 aagaatggaa tatcccttca aactggaaac tacgttcgca attagtattc ggttcaccag
      601 aaacaccggc tgaggaaaaa gaatacatga acgatgccga tcgcttccgc gtatttggat
      661 aattttattt gaaataaaaa acagaggttg tgacaaacat cttgtcgcaa cctctgtttt
      721 tattctggat caatcataat atcaatctct gcctcatcgt ttaagcgttg ggacaaaaat
      781 ggaactaatt cttctaatga tgcagaaaat ttcagcatcg gtcctcgcca tccgtttcgg
      841 aaaaaattca tttttacgat tttagaacct tttggctgat ataatacaat tttattgatc
      901 tcagaaaaag gaacaccttt tttgtcaaag ctattcaaaa caagtccttc ttctgtgatt
      961 cctctactgt ccaacaaaaa actaataaaa atcattgctg acaagatccc ttttgccaat
     1021 tcatctgctg taggatgact gatgagtgta taccaaatga ctagtgacac taagaagggt
     1081 atcaccaagt agacccatcg atttcgcaca gtccgataaa taatttcttt tcgaaccagc
     1141 caaagaaata aaaacatcac aacaacgaat aaaaacaata gaaccgtaaa gaatgttacc
     1201 atgacaactc gcctcgcttt ttcttctcca gaaaatagga aaagaatact gatttacctt
     1261 ctcaaagaaa ttggacttgc agcacgccgc acgtccaatt gttttcttta ttcaaatgta
     1321 tcatattttg caacaaccat gcttgctttt tcatctaata attcttggtc ctctgcagca
     1381 cattgggcaa ctttgaccac ggcgcagttt gtcatttttg atacaacttc accaatgacc
     1441 gtttttgtaa aaccaattgg tttgcatgca tattgttctc ctaaattaat catttcttcc
     1501 gtcatcaaaa tcctcctaaa ctgctggaac ttttctccac attaccactg ctatttcttt
     1561 ggagatttaa ctcattgatt tttatttaca gttaa
//
LOCUS       DASJUF010000231         1582 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.241, whole genome shotgun sequence.
ACCESSION   DASJUF010000231 DASJUF010000000
VERSION     DASJUF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.241"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(28..1509)
                     /locus_tag="U4B62_002979"
     CDS             complement(28..1509)
                     /locus_tag="U4B62_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2143049.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          501 a          216 c          279 g          586 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
     1561 gtggaaagtc atttcagtct gt
//
LOCUS       DASJUF010000232         1581 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJUF010000232 DASJUF010000000
VERSION     DASJUF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.242"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(64..1542)
                     /locus_tag="U4B62_002980"
     CDS             complement(64..1542)
                     /locus_tag="U4B62_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2143050.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          510 a          289 c          217 g          565 t
ORIGIN      
        1 tttctttgaa aaaaacataa atcagctaat aaatatgtga gacaaagctg ccatccataa
       61 atactagctg attttttcag ttaaatgtct atttttttct ctgatgatgg tatttccaaa
      121 atattcccag tcatctggag tcggtggatt tttccaaatt atctgttctt tattaaattt
      181 ttcaagtaaa caatcagata aataaatatt cgtacgagaa gtgattcgac tctcaatcac
      241 aatattgtaa tttttaaatc ctttcacttg agaaataatg taatcggtat aagcagatcc
      301 ttgcgaaaaa tcaatacaga catgaatcgg tgcttcaaaa tatgataccg gacaaatacc
      361 gaccaataaa aagagataat cataaaataa gcgaatcatg aaacgttcat cttttcccaa
      421 gaaatagcgt ttattctctt gtatgacctt ttggattgct gtactgaata ttggatacgt
      481 ctcgaagaaa aactgtagct gctttttaga cgtaaagcta gtcatctcaa agtcgaatat
      541 ccaatttttt cgattgacat gcatcatacc catacgaagt tcttttcgta tatatagtct
      601 ttcttcttca gagatttcaa tcgaaaatgg taattgatca atcaattcca ctgacatttt
      661 ttgactaatc atgtggatat catgaaaatg agcttccgca agtccaaatg cgccatctaa
      721 ttcaaattct tcttccgtga tcaaaaacaa catgatataa gaaatctcat caatgatctg
      781 ctctttaggg atatgaagaa aagaaatatg tgataaatcc ttgatgatct tcattccttc
      841 tgagctctta aagtattcat ctgaaaagaa acgttttccg attggttgat tcccataggc
      901 cctcaacaaa ctaatagtgg ttagcaaatt gaatttgatt cgttttgttg cagacagttt
      961 taaattgaac agatattcaa aatattgcac gatactcttt gcttcattca tgacgctttc
     1021 tctaaatggc atagaaaaac cattaaagac cccgaaatat accccaaata atacgttccg
     1081 tatgtttatt tcttccccaa ctaatatgtt tttctttaac tttatttgat gctgttcaag
     1141 aaaagagact aattctcttc tttttacata ggctcttgat atgcttaaaa aatgattctc
     1201 tgcaaatttt tcgatcgtta cttttgaatc cacgatctcc gttaacagtc gagcaatttt
     1261 tgatcgttga aagtagattc ttcttaaatt tttgatatcc gtcaaattca gacgttcgac
     1321 atggatctct gcttcttctg aaatttggat ctctggcttt gttttgaatt cagtaaattc
     1381 tttatttaat tctatgatat aatttcttgt tttaaattta gacaaaccta atgtttcata
     1441 taacttaggc agcactacat aattcttttt ctcaaattct aaatatctag ctagcagcag
     1501 tttttctcta tcttcctctt ctataaggta atccaacttc atatcctaca cctcaaaaaa
     1561 tgcctcatac caattacttg a
//
LOCUS       DASJUF010000233         1544 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.243, whole genome shotgun sequence.
ACCESSION   DASJUF010000233 DASJUF010000000
VERSION     DASJUF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.243"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            213..1382
                     /locus_tag="U4B62_002981"
     CDS             213..1382
                     /locus_tag="U4B62_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2143051.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          570 a          222 c          247 g          505 t
ORIGIN      
        1 gcaaagaaag tttaataaga gtttggacct agcaaacaat attaatgagg tcattaaatc
       61 ttattatctt tagtgatata acgaaatatc tacacagaca aaatgatagc gtaaaatatt
      121 ttgtgtaaat agatatttag ggtagaaaaa aagaaagtcc attctgtaaa attaaagtta
      181 ccacaaccga aattttatag gaggactttc tcatgcccaa ttttactaca gaaattatgg
      241 aaacactaat caataaaggt gatttagatg aattatttcg atgtcattta gaattagctg
      301 ttaattcatt attgcaggct gaattaacag cttttcttga ttatgaaaaa tatgatcgtg
      361 ctgggttcaa ttcaggtaat tcccgtaatg ggaattattc acgttcattc agaacagagt
      421 atggagaatt aaatttggta attcctagag atagaaatgg taagttttct caacaaactt
      481 tacctgctta taaaagaacc aatgactctt tagaaaccac tattattcag ctatttcaaa
      541 aaggaattac tatgtctgaa atctctgaat tgattgaaaa aatgtacggg catcactata
      601 cgccacaaac catttcaaat atgactaaaa tagtttctga agatattatt gcctttaaag
      661 aaaggtcctt agaatccaga tactctgtca tttttatgga tgctactcac attcctttaa
      721 aaagacaaac tgtttcaaaa gaagcagttt atattgtaat tggcattcga ttagatggaa
      781 ccaaagaggt tcttgggttt agtcttgctc caactgaatc tgcttatgtt tggaaagaga
      841 ttcttcaaga cctaaaagac cgtggtttaa aagaggtttt attagtcgta actgatggtt
      901 taagtggcat taacgatagt attcatagtg tttatccaaa tgctcaattt caacaatgtt
      961 gtgtccatat atctagaaat atcgctcata aggttcgtgt tagtgatcga caagaaatct
     1021 gtagcgattt caaattagtt tatcaagcaa gttcaaaaga agaagcaaat aatcaaataa
     1081 ggtttatgat agataaatgg aaaaagcagt atccacgagt ggttaaattg ctcatgaatc
     1141 ctgctatatt aaccttttat aatttccctc cgtcaatcag aagaactatt tattcaacta
     1201 atttgattga aggttttaac aaacaattaa agaaatatac caaaagaaaa gagcaatttc
     1261 ctaatgaaga atctctggag agatttctag tatctcaatt caacaactat aatcaaaagt
     1321 ttctatgccg agttcataaa ggttttaaag aaatacatga tacattagaa tcaatgtttt
     1381 aacttgatca acagaatggg cttttccatt tacacaaaat tattgacgct cccgacaaaa
     1441 ttatgatctt gcgtaaagtt ataaaatatt aataaaaata ttttattaat gaaaagcttg
     1501 gtagcacggt tattcatatg ctaatttatt tcactttata gtaa
//
LOCUS       DASJUF010000234         1536 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJUF010000234 DASJUF010000000
VERSION     DASJUF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.244"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="U4B62_002982"
     rRNA            complement(<1..>1536)
                     /locus_tag="U4B62_002982"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DASJUF010000235         1449 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.245, whole genome shotgun sequence.
ACCESSION   DASJUF010000235 DASJUF010000000
VERSION     DASJUF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.245"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            64..474
                     /gene="spx"
                     /locus_tag="U4B62_002983"
     CDS             64..474
                     /gene="spx"
                     /locus_tag="U4B62_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2143052.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            488..844
                     /locus_tag="U4B62_002984"
     CDS             488..844
                     /locus_tag="U4B62_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN2143053.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          536 a          210 c          270 g          433 t
ORIGIN      
        1 aataattggt attttaaaag aaattactgt agaattggat tataattaaa aaggagtgag
       61 aaaatgataa aagtatatac ttcgcctagc tgtacttcct gtagaaaagc aagagcatgg
      121 ttgcaagcga atcaacttga atttgaagag aagaatattt ttgccgaacc gttaacagaa
      181 agtgagatca aaaagatctt ggcagcgact gaaggaggag ttcaagatat tatttccact
      241 cggtcgaaaa tttacgaaaa gctgggcata gattttaacg aattaacttt aaagcaaatg
      301 atcgtcttat tcaaagaata tccttcatta ttgagacgac ctattttgat tgatgaaaaa
      361 agaatactaa tcggatttaa tgaagatgag atccggtcat ttgtccctcg tgaaattcga
      421 tgtgttgcca gaaatctgat ctatttacaa aacaataaca tcttaaattc atagggaggg
      481 agccttcgtg gaagtgaaag tctatggatc agcgcactgt gtaacaagtc tgaaggcgaa
      541 acaatggtta gaacaccatc aattgcctta tcaatttgtt gacttagaag aacgcgatct
      601 aagtacagaa gaagcagaag aattatgcag ttttgaagaa gtgcaagcag aacagctatt
      661 tgcgacttgg tctgaggctt ttcacaacat tgcgattgac ctttcctgtg taggaaaaaa
      721 acaactgatt tttttatgca cacaacatac caatctcctc agacgtcctt tgattattat
      781 cgatgaagtt ctatttattg gatacaatga gcagttgatg gaacagctta tcttaaataa
      841 atagacttta actgttttaa tcaagtcact gagcatttca tacagttgta acagtcgaac
      901 acttagcatc aagcgtgtta tgtgttcttt ttttgacttt atttttctac tatagagaca
      961 tgctcaattt atgtggtcaa aattcaaatc taggtgggga tatagagtta aaaatatatt
     1021 gacatgtgta aatttacatg atatattcat ttctgttgtt ttaagtagca aagcaataaa
     1081 atagtatttt aaaaaacata taaaatagtt gttgacatgt tagaaatgac atgataagat
     1141 aactgagttg ttaaaaaaga accacttgtg aagcttgatt ctatcgaaaa aaacttcaaa
     1201 aaagttcttg acaaataaca aatgataagt tatgataaat gagttgctgt aagatttcga
     1261 aaaacgaaat caaaaaaact tttaaaaaag agttgacaat tacttgtctc tctgatatga
     1321 tataaaagtt gctacggcag ccaaaaacga atcggggaac actcctgatg aagtagacct
     1381 ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga
     1441 caacaaaca
//
LOCUS       DASJUF010000236         1431 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJUF010000236 DASJUF010000000
VERSION     DASJUF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.246"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            197..460
                     /locus_tag="U4B62_002985"
     CDS             197..460
                     /locus_tag="U4B62_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2143054.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            460..732
                     /locus_tag="U4B62_002986"
     CDS             460..732
                     /locus_tag="U4B62_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2143055.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            809..1138
                     /locus_tag="U4B62_002987"
     CDS             809..1138
                     /locus_tag="U4B62_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2143056.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1128..1414
                     /locus_tag="U4B62_002988"
                     /pseudo
     CDS             1128..1414
                     /locus_tag="U4B62_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          534 a          175 c          283 g          439 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
      121 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      181 tagaggggga gtagatatgg caaaaacagt cttaaatcct agctcagcac ggaaaaattt
      241 ttataagttg ttgaaagatg taaatgagaa tcatacggaa attgaaatca ttagtgagcg
      301 cagtgaaaat aatgcggtgc ttattggttt ggatgattgg aaagcaatca aagaaacgct
      361 attacttgag caaataggca ctttgggtgt tgttcgtaag cgagaaaaag atgatagtgg
      421 atttactaat gttgatgaaa ttgattggga taacttataa tgaataacta ttctgtgatg
      481 attaaaaact cggctaaagc tgatttaaag aaaatcaaac agtctaattt gaaacctcaa
      541 tttgagaagg ttattcagac tttaaaggaa gatccctatc tccctaccca atcatttgag
      601 aagttaaaac ctacacatga aggtaggtat tctagaagat tgaatagaca acaccgggtt
      661 gtttataagg tagatgaaga tgaacacgtg gttgaaattt attcagcaag gacgcattac
      721 gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt actaagtaat
      781 tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac aaggcaattc
      841 aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa
      901 gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat tttatgattt
      961 tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg agattcttgt
     1021 agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg ctgaagatac
     1081 acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg gcttatgaaa
     1141 tcgttccttc aaaacatgtc attaagtatt taaaaagcta aaagagaagc cattgaaaga
     1201 aaaatttttg aatttaattt atgatgaaat tgcgattgat ccatataagg gtgaacaaaa
     1261 aactggggat ttatcaggta tttggtctag tgggttcaaa tatgcgggca caacatatag
     1321 agtagcttac gagattatag aaaacaaggt tattccagta ttgttatgtg gaacgcatga
     1381 aaacttttat gaacagttga aaaaattaat ctagtgattt gttgtcgttc t
//
LOCUS       DASJUF010000237         1427 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJUF010000237 DASJUF010000000
VERSION     DASJUF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.247"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U4B62_002989"
     CDS             131..505
                     /locus_tag="U4B62_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2143057.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U4B62_002990"
     CDS             complement(619..1248)
                     /locus_tag="U4B62_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2143058.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          481 a          214 c          250 g          482 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaa
//
LOCUS       DASJUF010000238         1417 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.248, whole genome shotgun sequence.
ACCESSION   DASJUF010000238 DASJUF010000000
VERSION     DASJUF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.248"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U4B62_002991"
     CDS             complement(150..1337)
                     /locus_tag="U4B62_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN2143059.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DASJUF010000239         1364 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.250, whole genome shotgun sequence.
ACCESSION   DASJUF010000239 DASJUF010000000
VERSION     DASJUF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.250"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1364
                     /locus_tag="U4B62_002992"
     CDS             82..>1364
                     /locus_tag="U4B62_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN2143060.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DASJUF010000240         1364 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.251, whole genome shotgun sequence.
ACCESSION   DASJUF010000240 DASJUF010000000
VERSION     DASJUF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.251"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(140..1288)
                     /locus_tag="U4B62_002993"
     CDS             complement(140..1288)
                     /locus_tag="U4B62_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2143061.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DASJUF010000241         1334 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJUF010000241 DASJUF010000000
VERSION     DASJUF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.252"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..403
                     /locus_tag="U4B62_002994"
     CDS             5..403
                     /locus_tag="U4B62_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2143062.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="U4B62_002995"
     CDS             387..1082
                     /locus_tag="U4B62_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEN2143063.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          506 a          200 c          266 g          362 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa caga
//
LOCUS       DASJUF010000242         1332 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJUF010000242 DASJUF010000000
VERSION     DASJUF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.253"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="U4B62_002996"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="U4B62_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN2143064.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="U4B62_002997"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="U4B62_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          510 a          219 c          218 g          385 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atctcttttt
     1321 caaaataaac at
//
LOCUS       DASJUF010000243         1317 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.254, whole genome shotgun sequence.
ACCESSION   DASJUF010000243 DASJUF010000000
VERSION     DASJUF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.254"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="U4B62_002998"
     CDS             7..624
                     /locus_tag="U4B62_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143065.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          497 a          168 c          206 g          446 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaac
//
LOCUS       DASJUF010000244         1314 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.255, whole genome shotgun sequence.
ACCESSION   DASJUF010000244 DASJUF010000000
VERSION     DASJUF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.255"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..278
                     /locus_tag="U4B62_002999"
     CDS             <1..278
                     /locus_tag="U4B62_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2143066.1"
                     /translation="EVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQ
                     CGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            283..1230
                     /locus_tag="U4B62_003000"
     CDS             283..1230
                     /locus_tag="U4B62_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN2143067.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          435 a          189 c          270 g          420 t
ORIGIN      
        1 atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca cctgaagttt
       61 ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa agtaattata
      121 tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta gatattgata
      181 gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa gattcttcgg
      241 agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg ttatcttatc
      301 accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga agaacatgtg
      361 gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat gacgaaaaat
      421 atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat catgaaagct
      481 aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga ttcagttgcc
      541 cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt agaattgatc
      601 cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc tgtttatggg
      661 gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac gccttatgcg
      721 gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata tgatgtaccc
      781 ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa ttctccatac
      841 agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg agaagcaact
      901 tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat agatgatgta
      961 atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca attcaatgtt
     1021 ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga aattctcggt
     1081 gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga ctctctagca
     1141 gatattacta agatccagtc aattggctat caaccgaaat atggtatttt aagtggaatg
     1201 actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta gagggtaaat
     1261 tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg gata
//
LOCUS       DASJUF010000245         1294 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.256, whole genome shotgun sequence.
ACCESSION   DASJUF010000245 DASJUF010000000
VERSION     DASJUF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.256"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1244
                     /locus_tag="U4B62_003001"
     CDS             <1..1244
                     /locus_tag="U4B62_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN2143068.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DASJUF010000246         1275 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJUF010000246 DASJUF010000000
VERSION     DASJUF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.257"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="U4B62_003002"
     CDS             complement(31..1203)
                     /locus_tag="U4B62_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEN2143069.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DASJUF010000247         1269 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.258, whole genome shotgun sequence.
ACCESSION   DASJUF010000247 DASJUF010000000
VERSION     DASJUF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.258"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..543
                     /locus_tag="U4B62_003003"
     CDS             <1..543
                     /locus_tag="U4B62_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2143070.1"
                     /translation="NFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILF
                     LIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFA
                     RKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFL
                     YPLSKDKVLENCELLKQKHK"
     gene            553..>1269
                     /locus_tag="U4B62_003004"
     CDS             553..>1269
                     /locus_tag="U4B62_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2143071.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLF"
BASE COUNT          452 a          138 c          242 g          437 t
ORIGIN      
        1 aattttatca atcctatttt ggttttaata gttgtttcgc ctttaacttt gtttttaagt
       61 aaaaaatatg gaaaaaaaga actggcatca gtaggaatgt tcttttcagc aatagtatat
      121 ttgatcttat ttttgataaa agctagtaat atgtatgtat tcttaattct tagttcggtt
      181 ggctatatgg gtttgggtgt gtttaatact gttatttggg caaatattac tgatgttatt
      241 gatgatcaag aagtaaagtc tgatcaaaga gaagatggga ccgtatatgc ggtatattct
      301 tttgctagaa aaataggtca agctctagct ggaggcgtcg gaggttggac tctttctatt
      361 ataggctatg actctttagc aaatgtacaa acgggagatg ttttagaaaa gttatataac
      421 gcttcaacat taattccatc aatttgtttt ctgattgtag gattaatttt atttttcctt
      481 tatccattat ctaaagataa agtcttagaa aattgcgagc ttttaaaaca aaaacataaa
      541 taggagagaa atatgaataa ttggatattt tgtattgatt cagatggctg tgttatggat
      601 acgatgactt ataaacacaa tctctttttc ggaccaattg ctgcagagat ttttgatgtt
      661 catcatagag aaaaattcct acaggattgg aataatatta atttatattc agagactcgt
      721 ggaataaaca gatttttagg tttagttaaa acattagaaa aaaatgatgt aacatcgatt
      781 aatcgattaa agcactgggt atctctaaca aaagaattgt ctaatccatc tttgaagagg
      841 gaaatagaat attctcagtc agaggattta ttaaaagctt taaaatggtc ggaagaagtc
      901 aatcaacaga ttagtcaagc aaaaggatta gataaaccct ttttaagtgc tagagatact
      961 ataaaagctc tgaaaaaata tgggaagata attattgtaa gctcagctaa taaggaagct
     1021 gttcaagaag aatgggaaag acatgaatta ttatcattag ttgatgaact gtgttgtcaa
     1081 gataaaggaa aaaaagaaga tattattcga agtgtaattg aaaatggttg tgatttggat
     1141 aagatattaa tgattggtga ttctcctggt gatctagaag ctgcaaataa aaataaagtt
     1201 ttcttttatc ctattttggt caataaagaa aaagaatcat gggaaaactt aagaactgat
     1261 gttttattt
//
LOCUS       DASJUF010000248         1257 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.259, whole genome shotgun sequence.
ACCESSION   DASJUF010000248 DASJUF010000000
VERSION     DASJUF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.259"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..511
                     /locus_tag="U4B62_003005"
     CDS             98..511
                     /locus_tag="U4B62_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN2143072.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          404 a          187 c          215 g          451 t
ORIGIN      
        1 ttcacaaagc taaccattat acttgcattt ttttcaaaat agagttatta cgtaaatagg
       61 gataaataaa aaatacttat tgaaaggatg atcaaaaatg gcaaatgtac ctagcattcg
      121 cgacatgttc cctgatttca atgacgtgtt tagtccagca ttcaatgatt ttttaggtgt
      181 ttcaagttat ccaaaagtag atttagtcga aaatgaaaaa gaatacaaac taactgctga
      241 tatgcctggc tgtgacaaag aagacacgac agttgaatat tcagataata ctttgacgat
      301 ctccgccaat catgagtccc atacagaaga taaagaagat ggcaactatg tacggaaaga
      361 aagacattcg gtctcttaca aacgttcatt ctatcttcct aatgttgatg aagaaaaaat
      421 cactggaacc tttaaaaacg gtgtgctgaa attggtatta cctaaaacag cttatcagcc
      481 aaaagaaaca aaaaaaattg agttaaatta gaacttatcg cctctcgatg aatgaggggc
      541 gatttttata tatttggata agaacgagta tgtttaaatt ttcaaaatga gcaaaaaaat
      601 attttgtttt tacgataaag atgttatgat ctctcttggt aaaaaatatg ggagttgatc
      661 atttgattgt cttaagtaaa cttttgtttt ggataccatt tatcataatg accagtgctt
      721 tcttttactt attcaaaagg cctaacaaat acttactctt atctgcttgt ccgatgatct
      781 attttatcca taagattaga ggattttatt attacgactc tggtatctca ttgttttgct
      841 acgaaatcgg cttattagct tcggccatct ttttcatatt tgtgttagaa tatttcagaa
      901 aaaaataaac ttccagtcag ttttggctgg aagtttttta tttggtgcag atcttttcag
      961 ctgttattca tacaagttga tccatttttc tattattaag tacataccat tgttactctt
     1021 tgaatgtggg ggagaaaaaa ataaaaggag ctggtatttt gttagttatg agtaaagctt
     1081 tgtttttact tccctgcacg ttcatgttta ttgttttatg ttatttcaaa agaagaaaaa
     1141 aatggtgctg gatcgatgtt ctactattcc ttcccttttt cccgcttatt tattttcttg
     1201 aagaaatatg ggatgatatg gcatgtgatt ctgcttttct ttttagtcat tcattgg
//
LOCUS       DASJUF010000249         1247 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.260, whole genome shotgun sequence.
ACCESSION   DASJUF010000249 DASJUF010000000
VERSION     DASJUF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.260"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..596
                     /locus_tag="U4B62_003006"
     CDS             <1..596
                     /locus_tag="U4B62_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN2143073.1"
                     /translation="TTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGD
                     FTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAEL
                     MDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHE
                     VEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            596..1213
                     /locus_tag="U4B62_003007"
     CDS             596..1213
                     /locus_tag="U4B62_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143074.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          457 a          163 c          267 g          360 t
ORIGIN      
        1 gaacaactca agacttctcc tattttgtca tcaatgctgt ttcaaaaaca gaaggagaaa
       61 tgggttctta ttggcaatca attgaagatt taattgctga tatgacagat tttattaatg
      121 aaaataaagg cgattttacg gactggatga atgaaaggaa agaagaattt gatcgctgga
      181 gagaagaaca ggagaatagt ttccaagatt ggagagaagg acaagaatca gattatttgt
      241 catggtttga atcaatcaag gatattttga agtctatcga tccaggtgga gtaatgttag
      301 ccgaattaat ggatgcacgt gtagatcttc aaggtgtacg ccatgaatcg atttctgaac
      361 gtttcctatc cgatctaaat tatttgtatc aaaaaatgaa agcagcactt ttcacgattg
      421 aatacggtga aattgaagtg actgatattc ttcaagacga tctcttttca gataatcacg
      481 aagtcgaaaa aattgaaaca gtagaatttc caattgaaga aggagctttg attattgcaa
      541 ctgttgatga tccaaaacag aatgttttca ctcttgagaa agttggggtg atttgatggt
      601 taaagtaaag agaatgatgg agactgatga atctggtgtt aaacgtcaat ttatgccaat
      661 tacacatgtt tctgcagttt taggcttaga aaaaatgata tcaggacaat caaaagttct
      721 ttcggttaat ggtaaatatg gtgcagtcat tttaacgaaa gctgatttag gattagagaa
      781 tgcaattact gaactacctt atgcaagcga gacaagcgat ggtattctta ctgctgaaat
      841 gtttcaaaag atcgtaaatg gagaaggtgg aacatatatt ttgccgattg ctactcctga
      901 acaattagga ggtataaaag ttggggaact cttagaagtt actgaagaag gtgttttatc
      961 tgcaactaag caaacagact tcaatttctc tgaagaatta aagggaaaat tagaatctct
     1021 aaaaatatta aaagcaggtg caaatatatc aattgcagaa gatggaacaa ttagttctac
     1081 aggtggtagt gggactggtg gtgttaatca gtcatatgta gatcaaaaat tccaagaagc
     1141 tgtaaatcaa gcagaaaatt acacaaacga aagaattcca aattttactt ttgaaaaaat
     1201 cggggaggta tagaaaatga cagatatcgt aaaaataaaa caaagcg
//
LOCUS       DASJUF010000250         1241 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJUF010000250 DASJUF010000000
VERSION     DASJUF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.261"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..989
                     /locus_tag="U4B62_003008"
     CDS             45..989
                     /locus_tag="U4B62_003008"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2143075.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          434 a          191 c          256 g          360 t
ORIGIN      
        1 tttcatgaaa gtaacaaata gttatgaaaa ggagctgttt attcatgaaa aaaacaagtc
       61 gtaaagtagt aatcgtcggt acaggttttg ttggaacaag tatcgcttat gcaatgatca
      121 accaaggaat ctctaatgaa ttagtattga tcgatgtgaa tcaagaaaaa gcagaagggg
      181 aagcattgga ccttttagat ggtatggctt ggggcgatga aaatgtggct gtatggtcag
      241 gtggctatga agaatgtaaa gatgctgata tcgttgttat cacagctggt atcaaccaaa
      301 aacctggtca atctcgttta gatttagtta aaacaaatgc gtcaatcatg cgccaaatcg
      361 taaaagaaat catgggatca ggattcgacg gtattatcgt tgttgcttca aacccagtag
      421 atattttgac atatatcgct tggaacgaat ctggtctgcc aacatcacgt gttatcggta
      481 ctggaacaac attggataca actcgtttcc gtaaagaaat cgcattgaaa ttaaaagttg
      541 acccacgcag cgtccacggc tatatcttag gtgaacatgg tgattcagaa gttgctgcat
      601 ggtcacatac aactgtcggt ggtaaaccag tgtttgaaat tgtagaaaaa gaccatcgta
      661 ttgcccaaga tgaattagat gtgattgctg ataaagtccg taatgctgct tatgagatca
      721 ttgatcgtaa aaaagcaact tactatggta ttggtatgag tactgcacgt atcgtaaaag
      781 ctatcttgaa taacgaacaa gctgtattgc ctgtatctgc ttatttaact ggtgaatacg
      841 acgaaaaaga tatctttaca ggtgtgccat caatcgttga cgaaaatggt gttcgtgaag
      901 ttgttgaatt atcaatcaac gaagaagaaa aagcaatgtt cagcaaatca acaagtgctt
      961 taagagaagt attgaacact gtattataag aaacaatgaa tatgaactaa aaatcctaaa
     1021 actgcttaac agctgaaata gctgttaagc agttttttat tgatatctaa taggtaagca
     1081 aataaacaga atggttaaaa ttaatcggtc cgaaaatgta tccgtacata tgaacatatg
     1141 aaatttaatc tgttgattac aataagcgaa tcgttatcaa tgtttatctg tggttaatta
     1201 aaaaatcctt ccgcaacggc aaatacaatg attcatatct a
//
LOCUS       DASJUF010000251         1226 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJUF010000251 DASJUF010000000
VERSION     DASJUF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.262"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..969
                     /locus_tag="U4B62_003009"
     CDS             <1..969
                     /locus_tag="U4B62_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2143076.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          407 a          223 c          250 g          346 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacag
//
LOCUS       DASJUF010000252         1225 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.263, whole genome shotgun sequence.
ACCESSION   DASJUF010000252 DASJUF010000000
VERSION     DASJUF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.263"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..747)
                     /locus_tag="U4B62_003010"
     CDS             complement(79..747)
                     /locus_tag="U4B62_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2143077.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            881..1168
                     /locus_tag="U4B62_003011"
     CDS             881..1168
                     /locus_tag="U4B62_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143078.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          467 a          200 c          201 g          357 t
ORIGIN      
        1 aagattttac tgaataatat cattttagtt tacataatga tatttctgta aattaaataa
       61 agtagtgaaa aataagaatc agtgctctaa ctgttcaaga ggatcaagtg gttcaaatgt
      121 ttctgtagtc tgttttgctt ccaattgggg caaaacagat ccaatttcca gatacttcac
      181 tttattcaat gtataagata gaatagaaca gctaagtaat gtatacagaa aatgtgtgag
      241 atccaaataa agcaaggaga caactaaaat accaaaagta atcaagaaaa gacttttgcc
      301 aatcttaata tgaaaatatt tttcaatggc ttgataaatg atatctgttc cgcctgtgct
      361 tccgccgaat cgaaagacga gaccgcaacc aatgccagaa agaatacctg tcatcaaact
      421 attgatcagt agactatcta cagcaaaatg tatatgaagg ctctcccata aatgtaaaaa
      481 gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc caaaaagaaa
      541 caatgagata agcaataaag gtaaattaaa aagcagtgaa gtgacagaag gagcgaatcc
      601 aaaaagatta tgcaaaagca aagcaatacc agtaacccca ccatctgcca gcttagatgg
      661 aatattgatt cctgtcacag ctaatccgta gattcctgcg cctaaaatga ttccgcacaa
      721 ttctgtaaat aaatttaatt ttttcatttc tagcctcttt tcttttgaaa attttcagca
      781 acttctttat tatactgact tatactctta cttcaagagg aaaatgaaaa aaaattattt
      841 ttggctgttt ctttttattt atcaaataag gagtggaaaa atgcgagcag cagacttatt
      901 gacaattctt acaaaatcac ccaaaaaaac agagtatcaa ggagtttttt tggctcaaga
      961 acaaaaactg gtgcctgtga caatggtcca gataaataaa gcaaatcaat ttatcttttt
     1021 atttgaacac ggcaaagcac cacttgctat gaaagacatc tttctttttt tgatgaagaa
     1081 ccgtgagaaa gctgtttatt atcagtcaaa agaagaactc attcctcttt atggagtgaa
     1141 agagatagaa aataaagtgg tgatataagt aaaaataaga aaacaagacg tttatgatgt
     1201 caaaacaacc attttttaca ttttt
//
LOCUS       DASJUF010000253         1201 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.264, whole genome shotgun sequence.
ACCESSION   DASJUF010000253 DASJUF010000000
VERSION     DASJUF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.264"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            54..857
                     /locus_tag="U4B62_003012"
     CDS             54..857
                     /locus_tag="U4B62_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2143079.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT          458 a          152 c          231 g          360 t
ORIGIN      
        1 gtaccagatt aacatgatta aaggagcgca aaaagaagat tggagagtaa tcaatgtctc
       61 aagaagttac cgaaaagatt attgatagtg atgtagattt agtatcagaa caaacaaatg
      121 caattgtgcg ttataacaat ggtctgaata caacagcttt gcgaggattt aagcctgttg
      181 aaatggatgt gttttgggct attttatcaa aaatgaaacg tcaaggaaca gcagaagtaa
      241 cttttgattt caacctcttt cgttatttgg tcaaatacga cagaagagaa aaggacagtt
      301 tttattctgt gttaaaagat ttgagtgaca aactaggtaa tttaacctac cgttttgaag
      361 attcagacgt ctatgaacag ctgtggcttt ttcaaaaatt ccggatagat aaacagaagg
      421 aaacagttac aattaaggca aatgaggaat ttgaatttat cttaaactca attggcaaaa
      481 actttactcg ttttgaactt gataatttta caaaactaag aagtagctac acaaaagaat
      541 tgtatcgtca tctcatgcaa tacagaaata aagagtggcg aaatggttat tgggtagtca
      601 aagttgaaga ttttagaaga gcattaagta taccttcaag ttatagaatg acaaatattg
      661 ataaaagaat actagagcag gcgaaagaag aatttttagc tcctgatgaa aacggaatac
      721 aggtatttga aacttttgat tatgaaaaaa tctatgcaaa gaaaggaaga agagttgatc
      781 ggctcagatt cacttttagt gagccagaat caaacttgcc aaccatttca atgcataatt
      841 ggttagaaga agattgagaa gaagcaggtt gtaattttaa tacaacctgc ttctttaaaa
      901 aagtatgggt aacacttttg gacaataaat gcaagaagtt tttttgtaag caagtttcag
      961 tctaaataat atttgaaaga atggctaatc tatacaaatt ttaacaacta tgggtaacac
     1021 ttttggacaa gtcaagaaaa aaaacaattt ttcttaattt gttgatcgac taaaggtaac
     1081 acttttggac aacacagaat aaaaagtccg gtgatatcag ttttggtagg aaacatctta
     1141 atatctttgt gagattgaag gtaacatttt tggacaagtc aactatttgt taaaagttaa
     1201 t
//
LOCUS       DASJUF010000254         1196 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.265, whole genome shotgun sequence.
ACCESSION   DASJUF010000254 DASJUF010000000
VERSION     DASJUF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.265"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(353..1057)
                     /locus_tag="U4B62_003013"
     CDS             complement(353..1057)
                     /locus_tag="U4B62_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143080.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          352 a          208 c          213 g          423 t
ORIGIN      
        1 agtctctttt tgagttgaaa cgaagaaaaa agtgttagta gaaaatcgct ttcgattttc
       61 caccaacaca aaatattata gaaccgcttt tttctctatc agaaaatatg tttattatta
      121 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
      181 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
      241 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
      301 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
      361 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
      421 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
      481 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
      541 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
      601 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
      661 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
      721 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
      781 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
      841 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
      901 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
      961 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     1021 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     1081 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     1141 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaata
//
LOCUS       DASJUF010000255         1195 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.266, whole genome shotgun sequence.
ACCESSION   DASJUF010000255 DASJUF010000000
VERSION     DASJUF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.266"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..>1195
                     /locus_tag="U4B62_003014"
     CDS             52..>1195
                     /locus_tag="U4B62_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2143081.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQ"
BASE COUNT          365 a          234 c          237 g          359 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaa
//
LOCUS       DASJUF010000256         1193 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.267, whole genome shotgun sequence.
ACCESSION   DASJUF010000256 DASJUF010000000
VERSION     DASJUF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.267"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="U4B62_003015"
     CDS             <1..1076
                     /locus_tag="U4B62_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2143082.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DASJUF010000257         1183 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.268, whole genome shotgun sequence.
ACCESSION   DASJUF010000257 DASJUF010000000
VERSION     DASJUF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.268"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..936
                     /gene="bsh"
                     /locus_tag="U4B62_003016"
     CDS             <1..936
                     /gene="bsh"
                     /locus_tag="U4B62_003016"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN2143083.1"
                     /translation="FGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAG
                     IADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEA
                     KKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPS
                     FDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTK
                     LNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTY
                     EDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          404 a          151 c          203 g          425 t
ORIGIN      
        1 tttggaagga attttgatta tgaaatatct tacaatgaag tagtcactgt tactccaaga
       61 aattataagt tgaattttcg aaaggtaaat gatttggata ctcattatgc aatgattggt
      121 attgccgctg gtatagctga ctaccctctt tattacgatg cgacaaatga aaaaggattg
      181 agtatggctg ggctaaattt ttctgggtat gctgattata aagaaataca agaagggaaa
      241 gacaatgtat ctccttttga atttattcct tggattttag gacaatgctc aacagtagga
      301 gaagctaaaa aattgttaaa aaatatcaat ttagcaaata taaattatag tgatgaactt
      361 cctttatccc ctttacattg gctattagct gataaagaaa aatcaattgt cattgaaagt
      421 atgaaagatg gacttcatat atatgataac cctgtgggcg ttcttaccaa taatccttca
      481 tttgactatc aattatttaa tttaaacaat tatcgtgtct tatcgagtga aactcctaaa
      541 aataattttt caaatcaaat aagtttgaat gcctatagcc gcggtatggg agggataggc
      601 ttgcctggag atttatcctc agtatctcgt tttgttaaag cgacttttac gaagctgaat
      661 tctgtatctg gagattcaga gtcagaaagt attagtcaat ttttccatat cttaggttca
      721 gtagaacaac aaaaaggttt gtgtgatgtt ggtgatggaa aatatgaata tacaatttat
      781 tcttcttgtt gcaatgttga caaaggaatc tattattatc gaacatatga agacagtcaa
      841 attactgcaa ttgatatgaa taaagaagac ttagatagtc ataagttaat tagttatcca
      901 attatagaaa aacaacaaat taaatatata aattagttaa tgtgttgtga ttgattattt
      961 aatatagata taatacaaaa aggcaaagat attttcaaaa cattttttct tgatgtgata
     1021 tccttgtctt ttttgtaatg tttttaactt attgcttttg caaaatttat ttacctgttt
     1081 tctggatcaa cgatagtgta tattatgcta tccttgtttg gattttagga cgtttaaaga
     1141 ggcacttttt tacataactt aatccataga ccttttaagc atg
//
LOCUS       DASJUF010000258         1154 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.270, whole genome shotgun sequence.
ACCESSION   DASJUF010000258 DASJUF010000000
VERSION     DASJUF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.270"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(110..562)
                     /locus_tag="U4B62_003017"
     CDS             complement(110..562)
                     /locus_tag="U4B62_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143084.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(569..1117)
                     /locus_tag="U4B62_003018"
     CDS             complement(569..1117)
                     /locus_tag="U4B62_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2143085.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          410 a          238 c          109 g          397 t
ORIGIN      
        1 catctatact catataatca ctccactaat taatctcccc aaaaagacac agcattaaga
       61 gatataccaa cacaacagtg caaataactc tacatcttcc ttcgcaacct tatttcaata
      121 aaccataaaa attatttttt aatagaaact gaaaattaaa actagaaaaa gtagttttac
      181 cgaatgtaag agcatcatca ccatcaatat agaaatacat tcttttccag ttacatttaa
      241 tttttaaaca aaattctccc caaaaaggaa ctttttctag tgaaaacttg gtaatctctt
      301 tgtaattaat tcttgttaaa gaataattaa acacttcatt tttacttgtt attttttcat
      361 tgactataac tagttcatct ggtaaaaaaa ttaaccaaca aacatcgaca aacagtaaat
      421 gatataatcc atttccacct ccaagcatat tcaatactgg tgaacatgcc ttagaaacac
      481 gaatatagtt atggtttaaa tagatatttt ctttaaacat tgcttgttta aaagccatat
      541 gctcttgttc taaacttttc ataaactttt actctaatgc acagctagca gcagctgctg
      601 caattcctgc tacaatattt accttagcta actctttagc tgcagctttt cctaattgtt
      661 tttctgcaat ttttaataat aaatccgcag ctttttcaaa attaaaagct tttaatgctt
      721 caataaaatc tccctgcgta cctatagccc aagccactcc tcctccagga agagaattaa
      781 tggctacaca tccagcatat cgtttccaat caactttctc cattctagga acaataatat
      841 gttcataaaa gactcttgct gctcttcctt gttcgttatc ttgactgatt cgttcacgaa
      901 tagcaccaaa atcctttata tggtatacac catgttcgtc taaaaaacca actgtatcaa
      961 aataaaaagt taattcatct actaatttat tctttatttc taaatcaact gtattcactt
     1021 tttctgtggc atacacactt gttccagtac taacaaaagt acttaaacct aataagaaaa
     1081 cacctaaaac accaaatatt ttaattattc ttttcataat ttctcctcca taaaaaataa
     1141 gttacttttt aaat
//
LOCUS       DASJUF010000259         1131 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJUF010000259 DASJUF010000000
VERSION     DASJUF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.271"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(490..663)
                     /locus_tag="U4B62_003019"
     CDS             complement(490..663)
                     /locus_tag="U4B62_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143086.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(666..1064)
                     /locus_tag="U4B62_003020"
     CDS             complement(666..1064)
                     /locus_tag="U4B62_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143087.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
BASE COUNT          424 a          167 c          124 g          416 t
ORIGIN      
        1 aaagaataat taaaaattta aatgttttaa tattacaaat gaaatcatgt tatcgtaaaa
       61 tggtataaaa aagctaaata ttctattttc gttattttat tagcaggaaa atttaaatag
      121 ttttacaaga gtccgtagaa atatattggt tttgccttca gattaatact ggatcatatt
      181 aatctgaata taatatatga actaataaat agcttaatct cgctattttg ttttttctga
      241 tcactatcta tacagcatta gtaaatagaa ttttaaaaat aaaaaattaa gacaaaaaaa
      301 gtatcaacat aaacatatac atatacacgt ttatgttgat acacttaatt tgtcctttaa
      361 aaagcactat ttttgatatt atgttatttt ataatcttat tacaagctga ttgttaggcg
      421 ttgtcctttc attacattta ttaacggtta ttttatcgtt taatttacta attatttttg
      481 atctaaaact tagcttaaca aataaataat taaccaaccg ccagataaaa atgcgatcat
      541 tgctaataca taagcaaaga taaaaatatg ttcgatcagc ttaaaccctg atgcgattaa
      601 gcagaccaag taaaatagaa attctcttac agataatggt aattttctaa ctagcatact
      661 cataattaac accccaaagt aatattgtat tttgaacatt ttacatccaa tacagcatca
      721 atcaaccatt gccaatcaaa aatacaaggc ttcattgaag tatgtttttc agatgaaaag
      781 atataatata atttcttcgg aataatattt tcagttgttg ggatcaagct aaatctaata
      841 ccgtctaact catagctacc agctttctct ccattaaaaa tttccttagc actttcttct
      901 aaagaatcaa aatatttttc gacagcaacc tttgaagctt ttactattga agcatttccc
      961 ttttcgataa aatctgcttt tgttttaccg acaataagta attcttgatc agtaaaatct
     1021 tctaaaataa tggtagtgta attcttattt cttctcaatc gcatcaggat cactccttaa
     1081 tataatttat gattttataa tatcataata agataaattt gcaagcattt a
//
LOCUS       DASJUF010000260         1073 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.273, whole genome shotgun sequence.
ACCESSION   DASJUF010000260 DASJUF010000000
VERSION     DASJUF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.273"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1073
                     /locus_tag="U4B62_003021"
     rRNA            <1..>1073
                     /locus_tag="U4B62_003021"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DASJUF010000261         1062 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.274, whole genome shotgun sequence.
ACCESSION   DASJUF010000261 DASJUF010000000
VERSION     DASJUF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.274"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..993)
                     /locus_tag="U4B62_003022"
     CDS             complement(37..993)
                     /locus_tag="U4B62_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143088.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DASJUF010000262         1060 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.275, whole genome shotgun sequence.
ACCESSION   DASJUF010000262 DASJUF010000000
VERSION     DASJUF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.275"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            523..669
                     /locus_tag="U4B62_003023"
     CDS             523..669
                     /locus_tag="U4B62_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2143089.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNT"
     gene            741..>1060
                     /locus_tag="U4B62_003024"
     CDS             741..>1060
                     /locus_tag="U4B62_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2143090.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKD"
BASE COUNT          410 a          151 c          179 g          320 t
ORIGIN      
        1 aaaggagcgt tctcaaatga gaacatgctc ctaaaactac tttattggcg tatggaaaaa
       61 ttatacttga agtggatagc gggttgaata agttgataag catcaaaaca tttataaaaa
      121 ttattcaaga ctatgtctgt tccatataca aatatgaaaa tcagtaaact ccatttaact
      181 tttttcgtag tgtgtctttt tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa
      241 ctatttttga tcattgacaa ttaaatacaa tttttgaata acgatccctg ctattcgagc
      301 tagaaactca agtacgccat gacctgatta gcgacaaata cttagagatt tctttttgtt
      361 ctgggaaaac aaaatgccat gacaatagca gcaaataatg atacttccgt aaggctcgcc
      421 gtaaactggg gagaggtgaa attcctatgt gcttacccaa agcaattcaa aaatttcata
      481 actactattt taggagatga ttttatagaa tccagagtct acctgaataa aaaacaaacc
      541 tgtgcatttc taggaatcga taataatact ctagataagt ggattcgtat atataatttc
      601 ccagtaataa gagttggtgg tgtagttcgc tttgacgtag aagatattaa agactttatg
      661 aatacttgaa attttcttct cgccgcatat actaaaatta gtgaaatata ctgccttact
      721 ttttttaagg agtgaggatt ttgcctacaa agctttcaaa tgggaaatat aaaactaatc
      781 tgagatatcc aaaaaggttt aaagaaataa caggaattgc atcagaaaag tatcaaaaaa
      841 catttccaaa taggcaattg gcgattaaag ccgaaaatga tatgaagaag aaaatagaaa
      901 aagttttgag agaggagaat gcaaactcat tagaattaaa aggaaaaata acgtttaaaa
      961 aattttacga atctaaatgg cttcctagat atgaattagg tcaaacaatt agatcaaaca
     1021 ggcctcctag tgatataacc attagtaata caaaagatat
//
LOCUS       DASJUF010000263         1042 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.276, whole genome shotgun sequence.
ACCESSION   DASJUF010000263 DASJUF010000000
VERSION     DASJUF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.276"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(3..248)
                     /locus_tag="U4B62_003025"
     CDS             complement(3..248)
                     /locus_tag="U4B62_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN2143091.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(363..941)
                     /locus_tag="U4B62_003026"
     CDS             complement(363..941)
                     /locus_tag="U4B62_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2143092.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT          319 a          200 c          153 g          370 t
ORIGIN      
        1 ttttaaactg cttcagttaa aaattcgatc tcaactgtcc gagtaagtac atctgaatag
       61 ctgtaagata ctcgttcaaa agaattttct tcaggatcca aatcaacaac gaatactgaa
      121 ggataggttt ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc tgtctgagca
      181 accaacatga ttgggctgcc gatacgacct tctaaatctt tcttgattga agctaaagtt
      241 gttggcatac agttcacctc tactatcaac tataccacat atttttaaaa ttttcaagtt
      301 taccataaaa aaaaaagaaa tgcaataaaa ataaataaga agctcattaa gatttttcta
      361 aattattcct cattataaaa tttcaagtgt ttttttattt tgagttttac tctcccgtaa
      421 gcgctcatta cttgcttgtg atccattgct aaacattcag ccatgtctac aaaatcaacg
      481 ccttgcatat aataatgaaa aattttagct tctatttttg aaaactggat ttcgcaattt
      541 tcgattgctt cttttaataa aagatgctga attgctaaag gttcttgcaa tgtgatctct
      601 gctaaaaagt ctggtccttc tgtctctatt ttttggtcga ttgagacttc atctttttga
      661 tcacgacgct tcagcgcgtt ctgcttcctc aacagagaat agatttgatt gcttataacg
      721 cttttataat aacttccaaa gctaacacct ttgctttttt cgtaagttct caatgcctga
      781 tataaagcga tccttgcttc ttgttcccaa tcctcagaaa cataatttct taaataatag
      841 gtgtttttca aacgtagtaa aagtggaaaa tatttttgat acagggcagc aaatgcttct
      901 tcgtcaccat tacgtgcagc gcgcaataac tcctcagaca taaaataatt cctccataaa
      961 ttcgtttatg gaggaatctt atcatcaatg attcatcgaa ctgattagaa cgatatgtta
     1021 tttatttttt ctcattaaat cg
//
LOCUS       DASJUF010000264         1002 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJUF010000264 DASJUF010000000
VERSION     DASJUF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.277"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            369..1002
                     /locus_tag="U4B62_003027"
                     /pseudo
     CDS             369..1002
                     /locus_tag="U4B62_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DASJUF010000265          961 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJUF010000265 DASJUF010000000
VERSION     DASJUF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.278"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..459
                     /locus_tag="U4B62_003028"
                     /pseudo
     CDS             172..459
                     /locus_tag="U4B62_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="U4B62_003029"
     CDS             473..904
                     /locus_tag="U4B62_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143093.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DASJUF010000266          961 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJUF010000266 DASJUF010000000
VERSION     DASJUF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.279"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..878
                     /locus_tag="U4B62_003030"
     CDS             <1..878
                     /locus_tag="U4B62_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2143094.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          301 a          212 c          192 g          256 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      961 a
//
LOCUS       DASJUF010000267          942 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.280, whole genome shotgun sequence.
ACCESSION   DASJUF010000267 DASJUF010000000
VERSION     DASJUF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.280"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(16..528)
                     /locus_tag="U4B62_003031"
     CDS             complement(16..528)
                     /locus_tag="U4B62_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143095.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(535..771)
                     /locus_tag="U4B62_003032"
     CDS             complement(535..771)
                     /locus_tag="U4B62_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN2143096.1"
                     /translation="MLYSPTEFKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRG
                     TYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          332 a          140 c          101 g          369 t
ORIGIN      
        1 ttaaaactaa catccttaat atcttaattt atccatattt tgagaaataa tacttttcca
       61 ttcagaaact aatttagtaa ggaatcttgc aatgttttgt ctcattaatt cattatttct
      121 attatctata agatctgtta gatatttttc aaattctaaa aagctatccg aattttcttc
      181 gacgattaaa tacttaagag aatttatatc ggaaaagata gattcaagtt tactaatctc
      241 attttctaaa tctatatttt cacgtgtgat tatctcatca ctttttctta aaaagctaaa
      301 aattgcatca gttgctttac acttttccat taaagaagta tattcttcaa tttttcttct
      361 ttctttatgg tcatttttta aatcagtttc aatgtctttt ttcatttgtt ctatactgag
      421 tttgttcttt ttgttctttt gagtaaatct aaaattcaaa cacgaaaaaa gtatgttagc
      481 aataacacct ataatagcaa ataaatcatt tatactattt aatttcattt atcatcacaa
      541 acttagaact ttacttatca tctcataaga caagtttcca aattgataat tattggtaga
      601 ttttaaatca ttataatttt cgagaaattt tatataagta cctctcatcg gtttatggct
      661 attaaaagtg ttaaaatcac tatcttcaac tatttcttga aacctattta atttttcaat
      721 aaaaaattgt gcataaagtg ctaacttaaa ttcggtagga gagtataaaa tattttgtgt
      781 aaatgaaaaa atccatacaa aaaaggaagt cccttctgta gaataaagtt aacgacaacc
      841 aattcacaga aaagaggact tccctatgaa tgattttact acagaaattg tgcaaactct
      901 agtcactaaa ggcgatttaa atgaattatt ccgttcgcac tt
//
LOCUS       DASJUF010000268          910 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.281, whole genome shotgun sequence.
ACCESSION   DASJUF010000268 DASJUF010000000
VERSION     DASJUF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.281"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..355
                     /locus_tag="U4B62_003033"
     CDS             <1..355
                     /locus_tag="U4B62_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143097.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="U4B62_003034"
                     /pseudo
     CDS             615..910
                     /locus_tag="U4B62_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DASJUF010000269          898 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJUF010000269 DASJUF010000000
VERSION     DASJUF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.282"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            256..879
                     /locus_tag="U4B62_003035"
     CDS             256..879
                     /locus_tag="U4B62_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2143098.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          332 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaaga
//
LOCUS       DASJUF010000270          856 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.285, whole genome shotgun sequence.
ACCESSION   DASJUF010000270 DASJUF010000000
VERSION     DASJUF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.285"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..452)
                     /locus_tag="U4B62_003036"
     CDS             complement(<1..452)
                     /locus_tag="U4B62_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEN2143099.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRV"
     gene            complement(457..642)
                     /locus_tag="U4B62_003037"
     CDS             complement(457..642)
                     /locus_tag="U4B62_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN2143100.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          264 a          166 c          105 g          321 t
ORIGIN      
        1 actctcaact tattgaatat ctgtttaata taatttggtg ataatggttg ctcaatgccc
       61 ttttttacaa catggatttt cgataaaaac tgttgaaatt cctgtatatg aattggtcga
      121 atctttacaa cctgcatttc tccaaaaaaa tccaatgcac gttgtaaaat atgatctgtt
      181 ttcatatatg ttttttctac agttcccaac ttataccaag gaaaaaaata ctctcgataa
      241 aaatcatgaa acgttattcc ttgcagataa attccttgtg aatcagaaaa actttgtttt
      301 aactcttcga gtccccttct agcttctcct tgagttttga agcctcttct ggtcacttga
      361 attctttttc caaagcgatc ttgtcctaaa gttgcagaaa aaaaccaagt gcctcttttt
      421 ctgtctttat aaacattcgc aattttcctc attttactat tcctcccaag aaatatggag
      481 aacttcttct aaaattgaaa taggaattct ttttattttt gaattacgat aaaaaaagta
      541 tcctttctca actaaaagtt ctttacattc cttgaagatt ttattagcat agtactgaga
      601 aaaacctaga ttaatcaaat cttttcgagt agctgttttc attatagcac cttccttact
      661 ttttttcatt tatttctatc agctgctttt gtcaaaagca cataggaatt tcacctctcc
      721 ccaagtttac ggcgagcctt acggaagtat cattatccca actttaaatc atcgcttttt
      781 attgactata aaaattattt tagaatattt atcgctcctt ctttacagca agaaatcatg
      841 gcgcaactaa aatatg
//
LOCUS       DASJUF010000271          851 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.287, whole genome shotgun sequence.
ACCESSION   DASJUF010000271 DASJUF010000000
VERSION     DASJUF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.287"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>851
                     /locus_tag="U4B62_003038"
     CDS             <1..>851
                     /locus_tag="U4B62_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEN2143101.1"
                     /translation="DNGYASFMTPFVWMFISSYEKLRSFLINERNISSLIQMEYSAFE
                     EATVPICTFTIKNSNEQDGSYIKLSDFKGGMEVQKIKTLEAIHQHGECDYFYSTNQDN
                     FKKIPGMPIAYWASDNVITDFAQGKLTNDVADAKQGLATGNNNKFLRYWFEVEFNKIK
                     FDAKTLEDAKKSRKKWFPTTKGGRFRKWYGNNEYVVNWENDGDEIKNYVNENGKLKSR
                     PQNLNYYFKKGITWSTLSSGQVSFRLQNQAIFETKGSVLFSKDITDFEEIFGYVNSKL
                     VNHFLTF"
BASE COUNT          319 a          108 c          157 g          267 t
ORIGIN      
        1 ttgataatgg atatgcttcc tttatgacgc catttgtttg gatgttcatt tctagctatg
       61 aaaagttacg ctcattttta attaatgaaa gaaacatttc tagtttgatt caaatggagt
      121 attcagcttt tgaagaagca accgttccaa tttgtacgtt tacgataaaa aattcaaatg
      181 aacaagatgg aagctatata aaattatcag attttaaagg tggaatggaa gttcaaaaaa
      241 ttaaaacgtt agaagcaatt catcagcatg gtgaatgcga ttatttttat agtactaatc
      301 aagacaactt caaaaaaatc ccaggaatgc cgattgctta ctgggcaagc gataacgtaa
      361 tcactgattt tgctcaagga aaattaacta acgatgttgc agatgcaaaa caagggttgg
      421 caaccggaaa taacaataaa tttttgcggt attggtttga agtagaattt aacaaaataa
      481 agtttgatgc taaaacatta gaggacgcta aaaagtctag aaaaaaatgg tttcctacaa
      541 ctaaaggtgg tcgttttaga aaatggtatg gaaacaatga atacgtggtt aactgggaga
      601 atgatggaga tgaaataaaa aattatgtta atgaaaatgg gaagttaaaa tctagaccac
      661 aaaatcttaa ttactatttt aaaaagggaa ttacttggtc gacactttca tctggacaag
      721 tatcttttag attgcagaat caggcaattt ttgaaactaa aggatctgtt ctgttctcaa
      781 aggatataac tgactttgaa gaaatatttg gctatgtaaa ctctaaatta gttaatcact
      841 ttttaacttt t
//
LOCUS       DASJUF010000272          838 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJUF010000272 DASJUF010000000
VERSION     DASJUF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.288"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>838
                     /locus_tag="U4B62_003039"
     CDS             <1..>838
                     /locus_tag="U4B62_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN2143102.1"
                     /translation="IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINF
                     NHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIK
                     GAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGI
                     ILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFM
                     APKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMI
                     FIP"
BASE COUNT          258 a          102 c          143 g          335 t
ORIGIN      
        1 atagggaaca tatatttttt gttttcaaat gctttttttg atatgaacca acttgtattt
       61 aatttgatgt tattacaaag ttttgctagt tttgtaatta gttatatcca gtcttatttt
      121 acgattaact ttaaccattt taaatatttg attttatctt ttatatctac ttttttgaat
      181 attgcaatct ctgtagcttt gatgcagaca ttatattttg atgataaata tgttggcaga
      241 gtagtcggcg gaacactgtc atttgtgttg ataggtttag taatttattt aagaataatg
      301 ataaagggtg ctcatttttt tgataaaaaa cagtggagat acgctttaaa aatctctttg
      361 ccgctaatcc cacattcctt agcaaatata gttttagcac aattcgatag aattatgatt
      421 aattcttatt taggtcctgc tgcgggggga atatattcct acattagtaa cataggtatt
      481 attctaagcg taatatggat gtctactaat aacgcctggg ttccatggtt ctatggtgag
      541 atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta actattattt agtagtcttt
      601 actttgatta cgatgattgt aatggtagtt tcgattgatt tagcaaaatt tatggctccg
      661 aaagactatt attctgggtt atctttagtg gttccgataa ccttaggcta tttctttcag
      721 tttttatata gtttaccagt taatgctgaa ttttatgaga aaaaaacatc gtttattgcg
      781 attggtacag tagcaagtgc tatagttaat atactattaa acatgatttt cataccta
//
LOCUS       DASJUF010000273          838 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.289, whole genome shotgun sequence.
ACCESSION   DASJUF010000273 DASJUF010000000
VERSION     DASJUF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.289"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..380
                     /locus_tag="U4B62_003040"
     CDS             <1..380
                     /locus_tag="U4B62_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143103.1"
                     /translation="EDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIE
                     DTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVV
                     KFNGTEIGRQIFDIKVNKPVEKN"
     gene            394..795
                     /locus_tag="U4B62_003041"
     CDS             394..795
                     /locus_tag="U4B62_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143104.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          305 a          112 c          151 g          270 t
ORIGIN      
        1 cagaagatgt agaaagtcag caatttggca ctaatgttat taataaagta attaatccag
       61 ctctttctat taatgcgcct ttaattccta ctgctttatc attttctgta acagctgtgg
      121 tgtctgagat agaggataca gaaaagataa aaatagtaga aattgaagtt ttaaacaaga
      181 atgaaaaaca aattttttct acgggtgaag tatcggtaag cttgcctcct caagttaacg
      241 atataaactt taatataaac gccagaaatg tcttggttga agaagcagga gagcattatg
      301 ctgttgttaa atttaatgga actgagattg gaaggcaaat ctttgatatc aaggtcaaca
      361 aaccagtgga aaaaaattaa ggagacgata cggatgcttg atatagtacc atcaaataca
      421 catcggagtg gtagcttaat aaattttgct gcgatttttg cttgcgcatc ggtaattcta
      481 gcatctccag ttgttgtgga tgcaagttct actccgaaaa ctaaaaatga taatcaactt
      541 gtaattacta caaaaaatga gattaacaca atttcaagta atgatggcaa aatttttgat
      601 gtgataaata cggttttaaa aagaaaagct gaaatcaata taaaatatga tgaagattta
      661 aacttgtatt tctttgttat caaaacaaca tctgaattgt tttcctcaga ttacgatgtt
      721 ttggatactc tcgataatgt attatctgac tataaatata tggggaaatc tgtagtagca
      781 actttggggg aataaaatgt ttgactttaa tgaatattta tctatttcca aacaaatt
//
LOCUS       DASJUF010000274          816 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.290, whole genome shotgun sequence.
ACCESSION   DASJUF010000274 DASJUF010000000
VERSION     DASJUF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.290"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            677..>816
                     /locus_tag="U4B62_003042"
     CDS             677..>816
                     /locus_tag="U4B62_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2143105.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHE
                     QL"
BASE COUNT          292 a          132 c          104 g          288 t
ORIGIN      
        1 cttttgtttc tctctttcct ttctacatca ttaaatagta ttttatacgt tatctaaaat
       61 actattcctc tgtcaaaata agctaactta cttcattata aaatatccac ttatcgcttt
      121 catgtatatt ccaccgtaaa aagtacattt tatcaccgag aactcatcat ttttcatcat
      181 gatgaatact atttacatat atctagttca aagacaataa ttattatgat ccgttattat
      241 ctaagcacta tacagataat aggaattttt cgtttctaaa tattccaatt tgggaatcca
      301 ataaaaattt tcgttcaaat acgtgtctat tcgaactctt ttctagttgt taattatgaa
      361 gggggaagaa ataaaaaata caatctatac acgaataaga atattatcca aaaggaggtt
      421 ttagaaaaga tttattatac gtaaaaagct caagacattt tcttagccat aaggacatgt
      481 ttgatattta agaagatata tgaattttct tttctaagta taaagataac tatccattat
      541 caactaaaaa aacatttttg aaaataaaca tcagcagtcc tatcaatgca tgtgagctta
      601 tagaagttat tcgcagcgca gcataattgc attagcttct tttcgtgttt tttgcctctt
      661 ttccctttaa atcttaatgg aggaggtgtg caatatgtca gataacgacg aacgcgtaaa
      721 tgcccagttg attcgattct ttgaaaaggt cattttgaac accgctaata cttattataa
      781 aaaacaagaa gaaattcaaa aacatgaaca acttga
//
LOCUS       DASJUF010000275          814 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJUF010000275 DASJUF010000000
VERSION     DASJUF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.291"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..143)
                     /locus_tag="U4B62_003043"
     CDS             complement(<1..143)
                     /locus_tag="U4B62_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2143106.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQS"
     gene            224..799
                     /locus_tag="U4B62_003044"
     CDS             224..799
                     /locus_tag="U4B62_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2143107.1"
                     /translation="MKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDDN
                     PTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIAN
                     YKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYV
                     MMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          257 a          150 c          157 g          250 t
ORIGIN      
        1 gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg aatgatttct
       61 acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc ttgtgaaaaa
      121 gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc tagtctacac
      181 accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat cgcatttatg
      241 ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt ctgaaatggg
      301 tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg ataatccaac
      361 tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc caactcgagc
      421 aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg atcagttcgg
      481 agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt ataaatacga
      541 gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa gagctgatgg
      601 gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg gctatcatgt
      661 atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc gtgcgacaaa
      721 cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc gttggtattt
      781 aaatattcca caacgataaa aaattccccc ctct
//
LOCUS       DASJUF010000276          747 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.292, whole genome shotgun sequence.
ACCESSION   DASJUF010000276 DASJUF010000000
VERSION     DASJUF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.292"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..659
                     /locus_tag="U4B62_003045"
     CDS             21..659
                     /locus_tag="U4B62_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2143108.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          232 a          119 c          124 g          272 t
ORIGIN      
        1 gaatagtagg agttattatc atgaataaca ttttaggaaa aaaaatagaa cttaaaaaag
       61 ggatattggg cactattgtc gttgtactcg gtttgttagg gttgctattt gcgccatcca
      121 ttattagcat tttttcttta actatcactg gatttatagc atttacactt gtttttggta
      181 aaaaagatac gaaaaaaatg ttctcgaaac ctgtcgctcc tgtcaaaaat atagccaaat
      241 atttttttat caatgtagta atcagtgccg cagtctcggt atttttgcaa caaattttga
      301 aatggggttt aacaggcaat ccaataaatg aggctttcag cccgttatta tttattattt
      361 tgcctatcat gatacttgga gaagaattgt tctcagtgta ttttctcgct attttttcta
      421 gtaagttcag cattcctgtc gctagtttct tgtcagcaat catctttgga tttatccatt
      481 acagtactta tgataatgga aatattttac acactgtagc acacatcctt ttgatacaag
      541 gggtggcaag attgttattc aaccaagctg ccattaaaag caactcgatc attacaagtt
      601 gggcagttca tgtgatattt gatttcacag ctatattgct agtcgtttta ttctcctaaa
      661 tttttataga aataatacat gctctattgc taagaaagca tactttattt cctttaaaca
      721 aaaagacaga tgtatcaatg gattaaa
//
LOCUS       DASJUF010000277          737 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.293, whole genome shotgun sequence.
ACCESSION   DASJUF010000277 DASJUF010000000
VERSION     DASJUF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.293"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..727)
                     /locus_tag="U4B62_003046"
     CDS             complement(<1..727)
                     /locus_tag="U4B62_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2143109.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGK"
BASE COUNT          250 a           84 c          136 g          267 t
ORIGIN      
        1 tttttccatt cacttttcta aaatgttttt ttaagctggc tggactgata aaaaaaattt
       61 gggctaattg ttcaagcaaa aaattttctg aaagatgttc ttctaaatat cgtatgattg
      121 gatcaactaa ttcatctgaa taaagaatat gctttttttt aggaatgact ttctcatttg
      181 aaggtattgt caataaagca agttcctgtt gataatgcct tggtaaggaa gacaattgaa
      241 ttttttgcag acaataagta actaataagg aaagcaaatg aatgctcatc tgttggcata
      301 gtatgaaaaa agaattgatt tcctgatcat aggaaatagc tggcttacta tgttttagat
      361 aatcaatttt gaactgttca tttttcagaa aagtaatgat agatgttatt tcttgatcta
      421 tagaaaaaga ttgtcccgta atatgtgcta ataattgctc atcaaaataa aactggataa
      481 tgataaaagt ttgtgtttcc ttttgagtag aaaagatttg gacttcttgc tgtttatcaa
      541 tgaataaaag ggaaggatta tctagttcta tagatttatg tgcaaattca gcatataaat
      601 tagcgctttc atttattagt agtcgaaaat agggagcagc agataaatga agatgggtat
      661 ctatggaata ggttagtgtt tttttgacaa gattggagga ttggttgttt gttaacaaaa
      721 gaaccacttt cctttct
//
LOCUS       DASJUF010000278          731 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.294, whole genome shotgun sequence.
ACCESSION   DASJUF010000278 DASJUF010000000
VERSION     DASJUF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.294"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..524)
                     /locus_tag="U4B62_003047"
     CDS             complement(324..524)
                     /locus_tag="U4B62_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2143110.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          268 a          115 c          112 g          236 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa a
//
LOCUS       DASJUF010000279          726 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.295, whole genome shotgun sequence.
ACCESSION   DASJUF010000279 DASJUF010000000
VERSION     DASJUF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.295"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(30..103)
                     /locus_tag="U4B62_003048"
     tRNA            complement(30..103)
                     /locus_tag="U4B62_003048"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Ile,seq:cat)
BASE COUNT          256 a          115 c          124 g          231 t
ORIGIN      
        1 acacagaacg attcaattgg ctacattaat gtaccctgta ggactcgaac ctacgaccgg
       61 acggttatga gccgtctgct ctgaccaact gagctaaggg tagtggttgt tgccacataa
      121 agccataaac aatcaaccag tagaatgcgt ggcaacaaac ctgttatcgc atatcttgga
      181 gtgtgactat ttatgagtga tagtgaagat atgcgtctcc atatatgtat ggttgagaca
      241 gataaacatg tatgaaaaac gttgttaaat caacgttttt ttattttggt caaggagaga
      301 aaagtagaga aatacaaaat tttttgcatt gattttgcat tcaaaaaaac attcaaacat
      361 ttagaaatcc ccaatacgta ttcaggaaaa tctattttaa ggtttacaaa aagaactttt
      421 gttcgtacac tctttttgag gtgaactttt atgctgatgg aaggaaaaac acaattatgg
      481 tttaagcatg ttccttcaaa taagtttttt aattataaac tctagagaaa ttaattcatc
      541 ctaacataaa ataattgttc actcaaaagt ttttctagaa caattaaaac agcaggcttc
      601 acattcgcta tacatttgta agaagtaata attcctgtac taaaaggcat aattgcttta
      661 attatttttt gacttctctt caaacacaag aagtagttgt gtaataattg cagaaataag
      721 gattcc
//
LOCUS       DASJUF010000280          725 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.296, whole genome shotgun sequence.
ACCESSION   DASJUF010000280 DASJUF010000000
VERSION     DASJUF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.296"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..>725
                     /locus_tag="U4B62_003049"
     CDS             28..>725
                     /locus_tag="U4B62_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2143111.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQA
                     AYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQ
                     CYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYD
                     QLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DASJUF010000281          709 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.297, whole genome shotgun sequence.
ACCESSION   DASJUF010000281 DASJUF010000000
VERSION     DASJUF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.297"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..600
                     /locus_tag="U4B62_003050"
     CDS             <1..600
                     /locus_tag="U4B62_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2143112.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          180 g          189 t
ORIGIN      
        1 accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc
       61 tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta ctatacgatc
      121 ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg gtttagtcat
      181 ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc tggcagctgt
      241 ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga aaaacgctta
      301 gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga agcctcctat
      361 attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat catttttggc
      421 ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg
      481 gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc agcattagaa
      541 gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt acattcataa
      601 acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg aaaatttagt
      661 cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaag
//
LOCUS       DASJUF010000282          707 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.298, whole genome shotgun sequence.
ACCESSION   DASJUF010000282 DASJUF010000000
VERSION     DASJUF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.298"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..461)
                     /locus_tag="U4B62_003051"
     CDS             complement(<1..461)
                     /locus_tag="U4B62_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2143113.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          225 a          128 c          101 g          253 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgat
//
LOCUS       DASJUF010000283          690 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.299, whole genome shotgun sequence.
ACCESSION   DASJUF010000283 DASJUF010000000
VERSION     DASJUF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.299"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="U4B62_003052"
     CDS             132..344
                     /locus_tag="U4B62_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143114.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>690
                     /locus_tag="U4B62_003053"
     CDS             506..>690
                     /locus_tag="U4B62_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN2143115.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          245 a          103 c          121 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct
//
LOCUS       DASJUF010000284          681 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.300, whole genome shotgun sequence.
ACCESSION   DASJUF010000284 DASJUF010000000
VERSION     DASJUF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.300"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          250 a          119 c           92 g          220 t
ORIGIN      
        1 gaaacaattt tttctgcttg ttacctaaat taaatcacaa ctaaaggtaa cagtcaacaa
       61 agatttgtta ctaatctaat tttctatttt ttcgccttcc aaatcaagaa aaccgaaggt
      121 aacgtcttat cctagtgtga tacctcaaat gtccactttt gttacctttg ctgtccaaaa
      181 ctgttaccct tactgtccac ttttgttacc tttactgtcc aaaagtgtta cctttatacc
      241 cctttaatac ttactctccc aagagattaa gcctccccta acagatatat taacagatat
      301 ttaacagata tattaacaga tatttaataa aggaggatta ctcctctctt atttcaaaat
      361 cttaaaaata aaaaatctta aaaaaaacta acactatcta ggagtttatt ttgcttgtgt
      421 aactttgaat aattgaaaaa aaggaaaagg aggaagcttc agcttcccct tttatttata
      481 tttataatat atattttaat ctttaaaact ttagcaacct ccaaaaaggc tttatatcaa
      541 tcttaaatag attttagggt ggacataaag cgatgcgaac atggacaaaa agcgatgtga
      601 atgtggacaa aaagcgatgc aaacgtggac aaaaagcgat gtggtattcc gaatatatcc
      661 aaaaaaagaa aaagtatgtg a
//
LOCUS       DASJUF010000285          662 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.301, whole genome shotgun sequence.
ACCESSION   DASJUF010000285 DASJUF010000000
VERSION     DASJUF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.301"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..448)
                     /locus_tag="U4B62_003054"
     CDS             complement(<1..448)
                     /locus_tag="U4B62_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2143116.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          195 a          119 c           90 g          258 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gc
//
LOCUS       DASJUF010000286          656 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.303, whole genome shotgun sequence.
ACCESSION   DASJUF010000286 DASJUF010000000
VERSION     DASJUF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.303"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U4B62_003055"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U4B62_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN2143117.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="U4B62_003056"
     CDS             complement(481..>656)
                     /locus_tag="U4B62_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143118.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DASJUF010000287          581 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.305, whole genome shotgun sequence.
ACCESSION   DASJUF010000287 DASJUF010000000
VERSION     DASJUF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.305"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            16..>581
                     /locus_tag="U4B62_003057"
     CDS             16..>581
                     /locus_tag="U4B62_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEN2143119.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          203 a          101 c          115 g          162 t
ORIGIN      
        1 gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga tgtgattatt
       61 gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca agaaatctta
      121 tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca agaatatggc
      181 aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg
      241 gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca
      301 gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct
      361 tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc
      421 ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa
      481 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
      541 cgcaataaat tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DASJUF010000288          559 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.307, whole genome shotgun sequence.
ACCESSION   DASJUF010000288 DASJUF010000000
VERSION     DASJUF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.307"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(243..392)
                     /locus_tag="U4B62_003058"
     CDS             complement(243..392)
                     /locus_tag="U4B62_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143120.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          173 a           86 c           86 g          214 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgat
//
LOCUS       DASJUF010000289          544 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.308, whole genome shotgun sequence.
ACCESSION   DASJUF010000289 DASJUF010000000
VERSION     DASJUF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.308"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(144..443)
                     /locus_tag="U4B62_003059"
     CDS             complement(144..443)
                     /locus_tag="U4B62_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2143121.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          177 a          102 c           64 g          201 t
ORIGIN      
        1 attaaaggat aaaatacaat ataaagaaac atctgtttct ttatttagaa taaaaaaata
       61 cagactctgt tcaaataaat gaacctagcc tgcattatga gattgactat actttttaat
      121 tcattgagat gaataactcg tttttataaa tgcccaaaag taatcggtgc cgaagccatt
      181 cctcgaccta attctttttc aaattttcct attgttgata tctccatata aaacggtgat
      241 actggtttag acaattctcc cctgattgct aacggacgta aactacgttc caaattattt
      301 atgactttag gataatactg acctttttct aagcctgtct ttgcgtctat caaaagtata
      361 cgttcctttt ctttgatctc tggatttaaa acgagatcat ataattttag taccatgttt
      421 tctatttctt cacttttaga catcacgatt tcctcccaaa aatatatttc ttcccactac
      481 attctataaa caaagtatac gctacatcta gattttttta aatcattact ggattgttaa
      541 aacc
//
LOCUS       DASJUF010000290          526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.310, whole genome shotgun sequence.
ACCESSION   DASJUF010000290 DASJUF010000000
VERSION     DASJUF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.310"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..172
                     /locus_tag="U4B62_003060"
     CDS             <1..172
                     /locus_tag="U4B62_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2143122.1"
                     /translation="NNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLY
                     HANEVLKIAEEK"
     gene            173..>526
                     /locus_tag="U4B62_003061"
     CDS             173..>526
                     /locus_tag="U4B62_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2143123.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLR"
BASE COUNT          205 a           60 c          101 g          160 t
ORIGIN      
        1 aaataataaa ataagttttg aagaaaacat tgttaaagaa atgcctgaat ctgctaatga
       61 tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca aagaaaactg
      121 gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat aaatggaaat
      181 tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa atttgaaata
      241 tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct atcaacaaaa
      301 acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt tagtagaatt
      361 gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat tagctacaga
      421 tactgtaggg aaaattgaag tgatacgtga ttcattctta caactagaaa gagaagataa
      481 tatatttgat ttaattatag atttagattt gacttcacct ttacgt
//
LOCUS       DASJUF010000291          526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.311, whole genome shotgun sequence.
ACCESSION   DASJUF010000291 DASJUF010000000
VERSION     DASJUF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.311"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..354
                     /locus_tag="U4B62_003062"
     CDS             <1..354
                     /locus_tag="U4B62_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2143124.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..>526
                     /locus_tag="U4B62_003063"
     CDS             355..>526
                     /locus_tag="U4B62_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143125.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DASJUF010000292          512 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.312, whole genome shotgun sequence.
ACCESSION   DASJUF010000292 DASJUF010000000
VERSION     DASJUF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.312"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..343
                     /locus_tag="U4B62_003064"
     CDS             <1..343
                     /locus_tag="U4B62_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2143126.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DASJUF010000293          508 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.313, whole genome shotgun sequence.
ACCESSION   DASJUF010000293 DASJUF010000000
VERSION     DASJUF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.313"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..508
                     /locus_tag="U4B62_003065"
     CDS             <1..508
                     /locus_tag="U4B62_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HEN2143127.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          155 a           89 c          110 g          154 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaa
//
LOCUS       DASJUF010000294          494 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.314, whole genome shotgun sequence.
ACCESSION   DASJUF010000294 DASJUF010000000
VERSION     DASJUF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.314"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..268
                     /locus_tag="U4B62_003066"
                     /pseudo
     CDS             1..268
                     /locus_tag="U4B62_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          184 a           73 c           87 g          150 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atcgccgatt gtaaaattaa
      361 gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat
      421 aaggagtgag tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag
      481 tcttatttca aaat
//
LOCUS       DASJUF010000295          486 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.316, whole genome shotgun sequence.
ACCESSION   DASJUF010000295 DASJUF010000000
VERSION     DASJUF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.316"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(28..426)
                     /gene="spxA"
                     /locus_tag="U4B62_003067"
     CDS             complement(28..426)
                     /gene="spxA"
                     /locus_tag="U4B62_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2143128.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          140 a           98 c           85 g          163 t
ORIGIN      
        1 gtcttttcca agtttcgtat tatccttcta taatccagcc atcaattgtg cttgacgtaa
       61 ttctaattgt cgtacatcgc gcggtaggaa ccgacgaatt tcatcttcat taaaaccaac
      121 ttgcagacgt ttatcatcaa tcatgattgg acggcgaagc aatccaggat tgttttgcac
      181 aagttccaat aattcttgta acggcagatc atctaaatcc atgttcagtt tttggaatac
      241 tttggaacgt gtcgagatga tttcttctgt tccgtcttct gtcatttgta aaatcgcttt
      301 taattcagaa ctgttcaatg gttctgaaaa aatattacgt tcaacaaatg gaatctgatg
      361 ttcttgcaac cacgcacgag cctttctgca ggaagtacaa cttggggaag tatataatgt
      421 taacaacgtg caccactcct tttcgtgata agaatcactt ttttgtaaca gaagatcata
      481 ataatc
//
LOCUS       DASJUF010000296          469 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.317, whole genome shotgun sequence.
ACCESSION   DASJUF010000296 DASJUF010000000
VERSION     DASJUF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.317"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(101..>469)
                     /locus_tag="U4B62_003068"
     CDS             complement(101..>469)
                     /locus_tag="U4B62_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEN2143129.1"
                     /translation="AISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVP
                     QNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADY
                     VTVSNDKDGVSQAFERILTI"
BASE COUNT          150 a           80 c           64 g          175 t
ORIGIN      
        1 tattcgcttc gtttgacact gaagaagtac tatttgtact ggaatttatg ctccattttt
       61 ccgtatttag aattctatgt tctatactac cttaattagt ttatatggta agaattcttt
      121 caaatgcttg agatactccg tctttatcat ttgaaacagt gacataatca gcttttttca
      181 tgatttcttg atgactattt cccattgcaa aactaatatc tgcactttca aacatagata
      241 agtcatttaa actatctccg atcgcgattc tcaaaggttt ctttaaattg tagattgatt
      301 ccaaacgatc aaacaccaca cctttagata cattttgagg tacgatttca atatttacag
      361 gtgccgattc agtaacagac acatttagca tctttttcaa atggttaaat atattagctt
      421 tcaattgacg attaggaata aaaataaaaa atttgtaaat gctgatagc
//
LOCUS       DASJUF010000297          468 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.318, whole genome shotgun sequence.
ACCESSION   DASJUF010000297 DASJUF010000000
VERSION     DASJUF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.318"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>468
                     /locus_tag="U4B62_003069"
     CDS             <1..>468
                     /locus_tag="U4B62_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN2143130.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DASJUF010000298          463 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.319, whole genome shotgun sequence.
ACCESSION   DASJUF010000298 DASJUF010000000
VERSION     DASJUF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.319"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>463
                     /locus_tag="U4B62_003070"
     CDS             <1..>463
                     /locus_tag="U4B62_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2143131.1"
                     /translation="KKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWLRK
                     KRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNN
                     LIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          159 a           91 c           94 g          119 t
ORIGIN      
        1 gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct atatcaaaat
       61 taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa ccttagatat
      121 ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac gactccataa
      181 acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg gctccgcctt
      241 tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg tgaagtatct
      301 taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat tttatcaaag
      361 tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa tatataaaaa
      421 gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DASJUF010000299          458 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.320, whole genome shotgun sequence.
ACCESSION   DASJUF010000299 DASJUF010000000
VERSION     DASJUF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.320"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..234
                     /locus_tag="U4B62_003071"
     CDS             73..234
                     /locus_tag="U4B62_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN2143132.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>458
                     /locus_tag="U4B62_003072"
     CDS             253..>458
                     /locus_tag="U4B62_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2143133.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVN"
BASE COUNT          176 a           48 c           80 g          154 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatca
//
LOCUS       DASJUF010000300          455 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.321, whole genome shotgun sequence.
ACCESSION   DASJUF010000300 DASJUF010000000
VERSION     DASJUF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.321"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>455
                     /locus_tag="U4B62_003073"
     CDS             <1..>455
                     /locus_tag="U4B62_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143134.1"
                     /translation="SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPI
                     TADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          184 a           67 c          106 g           98 t
ORIGIN      
        1 agtattgaaa gtggaacaac agtctttgca gtgaaagacg gcgtaagctt accagaagat
       61 aaacttccag tgttgaaagc aaaagatggt tatacagatg cgaaatggcc agaggaagca
      121 actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa actggatgat
      181 aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag tgactacaga attgaataag
      241 gaaccagacg catttgaggg aattaagaac aaagaagatt taccaaaaga tactaagtat
      301 acttggaaag aaaaagtaga tgttagcgca gctggaaata aaaaagggac tgttgtagtg
      361 acatatccag atggatcatc tgatgaagtt gaagtagatg ttacagtgac agacaatcgt
      421 tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DASJUF010000301          441 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.322, whole genome shotgun sequence.
ACCESSION   DASJUF010000301 DASJUF010000000
VERSION     DASJUF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.322"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..262)
                     /locus_tag="U4B62_003074"
                     /pseudo
     CDS             complement(26..262)
                     /locus_tag="U4B62_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          142 a           75 c           74 g          150 t
ORIGIN      
        1 tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa
       61 tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga
      121 tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa
      181 taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt
      241 caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa aaaagccatt
      301 tatgttacac tccaattgta atacccgcta aagcatacaa ggagagtgaa cataaatgac
      361 ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc atcaagaaac
      421 tccggttgct atcgttgcga a
//
LOCUS       DASJUF010000302          440 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.323, whole genome shotgun sequence.
ACCESSION   DASJUF010000302 DASJUF010000000
VERSION     DASJUF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.323"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            80..>440
                     /locus_tag="U4B62_003075"
     CDS             80..>440
                     /locus_tag="U4B62_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2143135.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYY"
BASE COUNT          194 a           60 c           64 g          122 t
ORIGIN      
        1 aagattaaat atataaaaag ccttcgggct ttttctttta acaattaata ggaacgtata
       61 ttcgaaagga atagaaataa tgtggttaga aaaaataaat gacaaaaaat ttaagtacac
      121 cgaacgttat acggatccct taactgaaaa aaagagaaaa gtgtccgtta ctttatctag
      181 caattcacgc caagcttgga atcaagctaa cttacttttg aatgaaaaaa tcgcagaaaa
      241 aataaaacga aatgaagaat taccactaac ttttggagaa ttaaaaacta aatgggatga
      301 aaaatacaag ccaactgtaa aagaaagttc ctatcgaaca actcaggttt atctatcatt
      361 aatttcaaaa tatataaaag atgatgtact tgtaaaaaac gttaatagta atcttataca
      421 agatatgcta gattactact
//
LOCUS       DASJUF010000303          437 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.324, whole genome shotgun sequence.
ACCESSION   DASJUF010000303 DASJUF010000000
VERSION     DASJUF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.324"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..437
                     /locus_tag="U4B62_003076"
                     /pseudo
     CDS             1..437
                     /locus_tag="U4B62_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          179 a           77 c           80 g          101 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattga
//
LOCUS       DASJUF010000304          421 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.325, whole genome shotgun sequence.
ACCESSION   DASJUF010000304 DASJUF010000000
VERSION     DASJUF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.325"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..355)
                     /locus_tag="U4B62_003077"
     CDS             complement(170..355)
                     /locus_tag="U4B62_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HEN2143136.1"
                     /translation="MQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRA
                     WRKMLDDELIPLENELR"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DASJUF010000305          412 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.326, whole genome shotgun sequence.
ACCESSION   DASJUF010000305 DASJUF010000000
VERSION     DASJUF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.326"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..381
                     /locus_tag="U4B62_003078"
     CDS             46..381
                     /locus_tag="U4B62_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143137.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          164 a           49 c           79 g          120 t
ORIGIN      
        1 acaatttcct aggaaaaata gttaacataa aaggaatgaa ttgttatgaa acaatattta
       61 aatgaatgga aagcgataga ggggtcatta gtaacgcaaa gaataaagga attacctgat
      121 tgcttagaga aggatcattt gtttcaaata cgagaaatgt tgaaaaaaga acagtttgat
      181 ccagaccaat ttttagtagt cgagtatcca actattggag tgtactgttg taaccatatc
      241 aatgatggaa aatattttat tattcaggag tatggaggac agttaactcc tttctatact
      301 acatgggaaa tgagtgaaga aggaatcaat aattttccat gtgaaagcat cgaagattca
      361 atttcacaca ccgaatgtta attgattata actataaaaa aggattgaac at
//
LOCUS       DASJUF010000306          411 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.327, whole genome shotgun sequence.
ACCESSION   DASJUF010000306 DASJUF010000000
VERSION     DASJUF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.327"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>411
                     /locus_tag="U4B62_003079"
     rRNA            <1..>411
                     /locus_tag="U4B62_003079"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DASJUF010000307          411 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.328, whole genome shotgun sequence.
ACCESSION   DASJUF010000307 DASJUF010000000
VERSION     DASJUF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.328"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          180 a           64 c           46 g          121 t
ORIGIN      
        1 tacttgatat tgatgagcaa cataagaaac aaataaacca attgtaagta aataaatcat
       61 gaatatagca gtaagtttag aactggactg ttcaacaatc ctatttatag ctaattttct
      121 atataaccaa ataaacatca gcaaaaacaa agaatcaagc attatctgga atactacata
      181 accataccct tttatccctt caaatgaaaa catatacgtc ataaccatgg aagtaataat
      241 agatctgaaa tcatcaataa gcatacataa tagtaattta tttaaaagca ctatattagt
      301 tttcttcttt tcctttaata taaaataact tccgattata ggtatagctg cggaaacatc
      361 agtaaataat tcaacaaaaa aagtgagtgg tatcaataaa catacaaaaa g
//
LOCUS       DASJUF010000308          397 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.330, whole genome shotgun sequence.
ACCESSION   DASJUF010000308 DASJUF010000000
VERSION     DASJUF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.330"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(112..>397)
                     /locus_tag="U4B62_003080"
     CDS             complement(112..>397)
                     /locus_tag="U4B62_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143138.1"
                     /translation="AVSRAYYSAFKKSDEYLKENYDIIYNGSSGKGSHQTVWNLFSTV
                     KELNTLGIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT          124 a           74 c           54 g          145 t
ORIGIN      
        1 ggacgcacgt agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga
       61 aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc attaggtgag
      121 tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt tcgtaatggt
      181 ttcattagga atatagtcag cacattttct tttctctaac attctatacc cactattatg
      241 tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg tttggtggct
      301 tccttttccg ctacttccat tataaataat atcataattt tctttcaaat actcatcaga
      361 ttttttaaaa gcactgtagt atgctcttga aacagca
//
LOCUS       DASJUF010000309          382 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.331, whole genome shotgun sequence.
ACCESSION   DASJUF010000309 DASJUF010000000
VERSION     DASJUF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.331"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          148 a           55 c           80 g           99 t
ORIGIN      
        1 aatattaagt attgttaaat agtgataatt agtaatatac gaattataag atacatttta
       61 tatttaatgt atgtaaataa ataaaatgac tgacggatat tgaatagtaa taaataacaa
      121 taattcagac ggatacagtg agtgaagtag cttgctacga acgaacaggc agaatgaaac
      181 ggtagtttca ttagagcaag tcgcttgcga cgtagcgtta ccactgatgt aactggcaag
      241 ttacatagag agagtttgct tgcaaacgaa cgttacccac ttgaatagaa aacaaaaata
      301 acatattagt gaattttgaa taggagccac aactcaacca accgcttgcg gtgggtgagt
      361 cccacggagg aaaagacgaa cg
//
LOCUS       DASJUF010000310          377 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.332, whole genome shotgun sequence.
ACCESSION   DASJUF010000310 DASJUF010000000
VERSION     DASJUF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.332"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>377
                     /locus_tag="U4B62_003081"
     CDS             <1..>377
                     /locus_tag="U4B62_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2143139.1"
                     /translation="FLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGE
                     FKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLT
                     EEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          137 a           66 c           62 g          112 t
ORIGIN      
        1 tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc gagaaacggt
       61 tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat acctagagat
      121 cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa cgatacattg
      181 gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat tgctgatttg
      241 atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat gactaaagtt
      301 ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta tgtcgctatt
      361 tttatggacg ctactta
//
LOCUS       DASJUF010000311          369 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.333, whole genome shotgun sequence.
ACCESSION   DASJUF010000311 DASJUF010000000
VERSION     DASJUF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.333"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..>369
                     /locus_tag="U4B62_003082"
     CDS             98..>369
                     /locus_tag="U4B62_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143140.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          139 a           52 c           70 g          108 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttt
//
LOCUS       DASJUF010000312          366 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.335, whole genome shotgun sequence.
ACCESSION   DASJUF010000312 DASJUF010000000
VERSION     DASJUF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.335"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>366
                     /locus_tag="U4B62_003083"
     CDS             <1..>366
                     /locus_tag="U4B62_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2143141.1"
                     /translation="LVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFV
                     MPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEP
                     YANKITQELKSYDVSYTTE"
BASE COUNT          148 a           46 c           72 g          100 t
ORIGIN      
        1 tttagtaggt gaagggtcac ttagaaataa actgaaagcc aaagcagtag aattggatat
       61 agagaaaaga gtaaaatttt taggagaaag aaacgatatt cccgagttat tatctgtgat
      121 ggatgttttt gtaatgcctt ctctttatga aggtctgcca atggttggat tagaagcaca
      181 agcagcaaaa ttaaaattag tcttagcaga tactatttcc aaagatacaa agttaacaaa
      241 taatgtggat tttgtatcat tagatgaaac accacaagaa tgggcaaaaa aagtaataaa
      301 agagccgtat gctaataaaa taacacaaga actaaagagt tatgatgttt cgtacactac
      361 tgaaca
//
LOCUS       DASJUF010000313          364 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.336, whole genome shotgun sequence.
ACCESSION   DASJUF010000313 DASJUF010000000
VERSION     DASJUF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.336"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            42..>364
                     /locus_tag="U4B62_003084"
     CDS             42..>364
                     /locus_tag="U4B62_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HEN2143142.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHS"
BASE COUNT          131 a           64 c           55 g          114 t
ORIGIN      
        1 ttcttattgt aacgtatcga aaacaggtct atacttattc aatggaatcg taccagttcc
       61 aagtttttaa gctcgtacca agcttagaaa gaagaggcaa tcgtatgaaa ataacagaaa
      121 agaatgtaaa cccgaaattt gaaactaccg aaagcaatct aaaaaagttt gatttaactg
      181 ttactttatc gaatcaactt gtagatagca ttgttgaaat ggatgctatg ccacaaaaaa
      241 tttatcttta cgctatagga catcttgacc ctaaaaatcc gccttttaat aataccgtca
      301 aagtatctaa aaaggcttta tttgcgttga tgtcttctag ttcatcttct tcaaaacatt
      361 caag
//
LOCUS       DASJUF010000314          362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.337, whole genome shotgun sequence.
ACCESSION   DASJUF010000314 DASJUF010000000
VERSION     DASJUF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.337"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>362)
                     /locus_tag="U4B62_003085"
     rRNA            complement(<1..>362)
                     /locus_tag="U4B62_003085"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DASJUF010000315          337 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.343, whole genome shotgun sequence.
ACCESSION   DASJUF010000315 DASJUF010000000
VERSION     DASJUF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.343"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          124 a           38 c           72 g          103 t
ORIGIN      
        1 aaaattcatt ggtcgttgaa tgggctgaat agtagtgtat aaaacaattt tgtggagcgt
       61 gaagctttta tggtagagga aaatacgaat aaagtgatgt tgataaaaag attcctatct
      121 tgaatatgaa tcgagatgtc ttgaaaatga atgagtttga ccaatcatta tcctatcgaa
      181 atttacagtt tcgttcgatt ggtctgtcaa ttaatgaaaa agtaaaaaag aatatgttct
      241 aggaagaggg cggcacatct aaatttttaa gtaacggaca gttcaaatat caagcattga
      301 aagaggctat cctactgttt gaaggaatac gaatata
//
LOCUS       DASJUF010000316          335 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.344, whole genome shotgun sequence.
ACCESSION   DASJUF010000316 DASJUF010000000
VERSION     DASJUF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.344"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>335
                     /locus_tag="U4B62_003086"
     CDS             <1..>335
                     /locus_tag="U4B62_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEN2143143.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
BASE COUNT          125 a           53 c           77 g           80 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgag
//
LOCUS       DASJUF010000317          323 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.346, whole genome shotgun sequence.
ACCESSION   DASJUF010000317 DASJUF010000000
VERSION     DASJUF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.346"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U4B62_003087"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U4B62_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN2143144.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DASJUF010000318          315 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.347, whole genome shotgun sequence.
ACCESSION   DASJUF010000318 DASJUF010000000
VERSION     DASJUF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.347"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>315
                     /locus_tag="U4B62_003088"
     CDS             <1..>315
                     /locus_tag="U4B62_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143145.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DASJUF010000319          283 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.353, whole genome shotgun sequence.
ACCESSION   DASJUF010000319 DASJUF010000000
VERSION     DASJUF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.353"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..283
                     /locus_tag="U4B62_003089"
                     /pseudo
     CDS             1..283
                     /locus_tag="U4B62_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DASJUF010000320          279 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.denovo.355, whole genome shotgun sequence.
ACCESSION   DASJUF010000320 DASJUF010000000
VERSION     DASJUF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.denovo.355"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           93 a           47 c           43 g           96 t
ORIGIN      
        1 aaaaataatg ggaaaaagac ttataattgg agtggtagtt aattagtgac ttatttttga
       61 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      121 cttaaaaaat atctcctttc acaagtatgg tgataaaatt cgttccgggc taccttttta
      181 ggtagcctac tttaatcttt atacctttct ggatcaacga aagtatactt tatatagtca
      241 taacgccgat gatcgctacg tgcgtccggc acgtcagtc
//
LOCUS       DASJUF010000321        11089 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.091, whole genome shotgun sequence.
ACCESSION   DASJUF010000321 DASJUF010000000
VERSION     DASJUF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.091"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..624)
                     /locus_tag="U4B62_003090"
     CDS             complement(<1..624)
                     /locus_tag="U4B62_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2143146.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIF"
     gene            883..2385
                     /locus_tag="U4B62_003091"
     CDS             883..2385
                     /locus_tag="U4B62_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN2143147.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2387..3124
                     /locus_tag="U4B62_003092"
     CDS             2387..3124
                     /locus_tag="U4B62_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2143148.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(3295..4488)
                     /locus_tag="U4B62_003093"
     CDS             complement(3295..4488)
                     /locus_tag="U4B62_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2143149.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(4567..4773)
                     /locus_tag="U4B62_003094"
     CDS             complement(4567..4773)
                     /locus_tag="U4B62_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2143150.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(4766..5908)
                     /locus_tag="U4B62_003095"
     CDS             complement(4766..5908)
                     /locus_tag="U4B62_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2143151.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            6420..7055
                     /locus_tag="U4B62_003096"
     CDS             6420..7055
                     /locus_tag="U4B62_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2143152.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            7209..7286
                     /locus_tag="U4B62_003097"
                     /pseudo
     CDS             7209..7286
                     /locus_tag="U4B62_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            7531..7647
                     /locus_tag="U4B62_003098"
     CDS             7531..7647
                     /locus_tag="U4B62_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN2143153.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            7663..9582
                     /gene="tet(M)"
                     /locus_tag="U4B62_003099"
     CDS             7663..9582
                     /gene="tet(M)"
                     /locus_tag="U4B62_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEN2143154.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            9701..9868
                     /locus_tag="U4B62_003100"
     CDS             9701..9868
                     /locus_tag="U4B62_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN2143155.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(9928..10281)
                     /locus_tag="U4B62_003101"
     CDS             complement(9928..10281)
                     /locus_tag="U4B62_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2143156.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            10785..>11089
                     /locus_tag="U4B62_003102"
     CDS             10785..>11089
                     /locus_tag="U4B62_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEN2143157.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYF"
BASE COUNT         3420 a         2013 c         2150 g         3506 t
ORIGIN      
        1 aaaaatctcc acgacatcac cgcgcactcg aaaacgaccg cgctgaaaat ctatatcatt
       61 tctttcgaat tggatactga ctaagtcacg aatcaactga ttgcgatcta gttctgctcc
      121 ctgtcgaatc gaaactactt gtttttggta ctcaaaaggc gagcctaatc caaaaataca
      181 tgaaacggat gcaatgacga tcacatcatt tctttctaat aatgaactag tagccgaatg
      241 tcgtaatttg tcgatctcat catttacact tgaatctttt tcgatatacg tatcgcttga
      301 aggcacataa gcttcaggct gataataatc ataataagaa acaaaatatt caacagcatt
      361 gttagggaaa aattccttga attccccgta cagctgacca gccaatgttt tgttatgggc
      421 aataatcagt gttggtttat tcactttctc aataagattg gaaatcgtat atgttttccc
      481 tgttccagtc gcacctaata agatttgtgc ttttttcccg ccgactacgc catccactaa
      541 ttggttaatc gcttcaggct gatcaccagc cggttggtat ttcgagacaa gctcaaagtg
      601 tcgggaggta tctctctcaa tcattcttcg tcctcctttt cattcattcc ttttcatttt
      661 acgaccaata aaaagaaaag gaaagcaaag gcattcctta ctaaaaaatt ataccacacc
      721 gaacatactt tcgccacttt tttatcttat tatacatgtt atgttttata acataaaatg
      781 ttagaaactc gcattttttt cattttttcg agcttacagt cttgtctgat tcagcacttt
      841 tcgcctataa taataaaata ttggaaattt aggaggttga ttatgaaaag aaagcattta
      901 tttcactcat ttgttttctt aatgacagga cttgtgacac ttgcgtttgg tcatgttgca
      961 caagctgaca acactacaac ggcagagtca agcacaagcg agacaatgca aaaaatagag
     1021 cccaaaaaag acgtatacgt aattgccagt gactctgctt ttgctccatt tgaatttcaa
     1081 aacgcaaagg gcgagtacga aggcatcgat gtaaaattga tggaaaacat cgccaaactg
     1141 caaggattta acatccgaat ggactaccgt ggtttctctg ctgctttgca agctttggaa
     1201 tcaggacaag cagacggaat gattgccgga atgactgtaa cagatgaacg aaagaaaagc
     1261 tatgattttt ctgattctta ctttgatagc gggatccaat tagctgtcaa aaaaggagat
     1321 acttcgatca agtcttatga agatttgaaa ggaaaaacag tcggagcaaa agttggtacc
     1381 gaaagtgctg aatttttaag cgaccatgaa tctgaatatg gctattctat caaatatttt
     1441 gatgctgctg atcaattgta cgacgggtta aaagttggcg caatcgatgc aatgatggat
     1501 gattatccgg taatcggtta tgcgatcaaa caaggacaag aattggaaac accgatcaaa
     1561 aaagaatctg gcggaaaata tgcatttgcc gttaaaaaag gacaaaatcc tgagcttctt
     1621 aatatgttca atgaaggatt gaaagaatta aaaagaacag gcgaatacga taagattatt
     1681 aaagaatatg tagcggatgg ttcagaaact tccagtgagt cttccactgc tgatgaatca
     1741 acatttcttg gacttatcca aaacaactat caagcattgt tgaaaggact ttggagaaca
     1801 attctcctaa ctttgatctc ctttgctttg gctatagtaa tcggtgtgat ttttggatta
     1861 ttcagtgtag caccaatacg cgctcttcgc acaattgcta gtatttatgt cgatatcatc
     1921 cgaggaattc caatgatggt tcttgcgttc tttattttct ttgggttgcc tggtatcgtt
     1981 ggatttacga ttcctgactt ccttgctggt gttatcacct tgactcttaa cgcaagtgct
     2041 tatatcgcag agattgttcg aggcgggatc aacgccgttc ctgtcggaca aatggaagct
     2101 tccagaagtt taggtctttc ttataatcgg acaatgcaaa aaatcatttt gccgcaagcg
     2161 atacgcatca tgatcccatc attcgtcaat cagttcgtta tctcattaaa agacacaacg
     2221 atcatttccg caatcggtgt agtcgaattg ctgcaaactg gtaagatcat tgttgcacgt
     2281 acaacacaaa gtacgtatgt ttacttgatc attgcgatca tgtacttgat tttgatcaca
     2341 gcattgacaa aattagcaaa agttttagaa aagaaggtga agtaagatgg cagaaaaaat
     2401 cttagttgag catttagtca aaaaatacgg cgataacaca gtattaaacg acatcaatgt
     2461 atccatcaac gaaggagatg tcgtctgtat cattggccct tcaggttcag gaaaaagtac
     2521 attcttgcgt tgcttgaatc gtttagaaga accttctgac ggtgaaatta ttattgacgg
     2581 cgcccacttg atggataaaa gcacagatat caacttggtg agacaacata tcggaatggt
     2641 attccaacac tttaacctat tccctcatct ttctattttg gaaaacatca ttttagcacc
     2701 gatggacctg aagaaagaat ctagagaaga agcagagaaa aaagcaatca aattactgca
     2761 gacagttggt ctagaagata aaaaagatat gtatcctgaa atgctttctg gtggacaaaa
     2821 acagcgtgtg gctattgctc gtgctctagc gatgaaccct gacatcatgc tttttgatga
     2881 gcctacttct gcactcgatc ctgaaatggt cggtgatgtc ttgaacgtca tgaaaaagtt
     2941 agcagaacaa ggtatgacga tggttatcgt tacgcacgaa atggggttcg ctaaagaagt
     3001 ggccaatcgc gtcatgttca ttgacgacgg aaacttttta gaagatggaa cgcctcaaca
     3061 aatctttgaa aatccgcaaa atgaacggac aaaagatttc ttgaataaag tattaaatat
     3121 ttaaaataaa ttatatatgt ctttttgact atctttttat atcttcttaa atgtccgcaa
     3181 agccttattt tataggctct acgggcattt tgcttttgtg gtaaacctca catatctagg
     3241 tctatcttct tatattttca ctatcaagcg tggttaaaat cgtggtaaat acttctatgc
     3301 aattagacgc tgaacctctg attttgcggt atctatggac gcatgagcat accagttcat
     3361 tgtgatactg atatttgaat gccccatgat atactgtaaa tcttttgggt tcatgttctt
     3421 gcttgccagt cttgtgcaga atgtatggcg tagcgtatgc ggtgtgatat gtggcaatgg
     3481 attgtccttg tggtgcttgt tgtatttctt taccatacgg ataaataagg cgttataatc
     3541 aatcgcaact ttgggcttgc ccttatgatt gacaaatagg aagttgcccc gtccgtctat
     3601 cacaaatggt tctgcctttg gacgtttttt cataacctgt tgaaatgcct gtatcgtttc
     3661 tttgcttaat ggtacttgcc ttattccgct ttttgtttta ggcgtttcaa tataatagcc
     3721 ctgttccttg ctctttagta actggtggtc gataatcaca acttcattct tgaaatcaat
     3781 gtcggctact gtcagtccgc acagttccga aatacgaagt cctgtcttta acagtatcag
     3841 cacatcatca taatgcttgt gatacacatt gtccgtcttg atgaatgaga gtaaggcttg
     3901 ttcctgttcc tctgtcaatg cgactttctt tttggtatca ttttctagga cttcactcaa
     3961 cttgaaatca aagggatttt tcctcacata atcatcttga atggctatgt agaatgacgc
     4021 ttttaacgag cgtttatggt tgttaatggt attgtaggaa aagcctttgt ctttcatgcg
     4081 taacgcccat tccttagcgt cagagggttt gattgtatca atgctcctag cacctaactt
     4141 gtcctctttc aataaccgca tgagttgttc ccgttgtttc tgtgtgcttt tcttcacatt
     4201 tgccctctgt gcgttctgtt tggcataaag ttggcaaagc gtcattttct tgccagtgct
     4261 gtcgataccg tcctcaatat cccgtcttat ctgctgttcc agttctcgaa gtgagggttt
     4321 ttcccgtttt ccctttggtg tcgggtctgt gggtgtcaat ctccaagcat acacatattt
     4381 tgtgtttcca aatgcgtcta catatttata taagtatttt ctgtctgttc gttggctctc
     4441 tccagtatgc aggatacgac ctttgttatc ccgtcttttt tctttcatgg tgtctgctcc
     4501 tttccttgtt ggaaagagcc ttgatatgac ttgtgttcat cataacacat acaaggctca
     4561 tttgcattag atagcgtcca atttatcaat gacctgttca aactgtcggc gtttaatctg
     4621 tatgcggttg ccattcataa tgagccagcc agcgtccttg ttttcctctg ccaatcgtct
     4681 taacttgttt tctccgatac ggaaatactt tgacgcttct tcaatggtaa gggtgtattt
     4741 ttcccatacg ggaatatcct gtgcattact cataaaacac cccctcactt tcgattacgt
     4801 ctgctatgtc cgtacacatc tgttccacaa gcatttcctg cttttcagag aggtgggtac
     4861 attcatcaat cattttcatg tagtttgagc ctgtcagcct gtcgatttcc tctatcatca
     4921 cgattgacgg catgacctgt ctttccagcc attgcagagt gcgttccata ttgacaggta
     4981 cggggttcat ttcaaaaggt aaactgtcat gggaaatgaa tatctcccac agcggatagt
     5041 cgggaaattg cacaaaatca gtaataaggt aaaacaccag cccatgaggg ttatatgtca
     5101 ataacaattc ctcaactgcc tgtactgctt ttgtaccacg cagacgaatt tcaaaacgat
     5161 tgataatatc cgtatgtttc tttttggttg cctgttcctt ttgtttttca tacaggcaga
     5221 agtattttgt gcttgccttt gaaccgatat aaagggtgct tgctaaatta cggttttttc
     5281 cgcttagttt tccaccctgc acattttcat aatttttaca gcgacaatcc gcaccgccat
     5341 ttctgtattt ttcagacaaa acgggaatat ccaacagacc acacatatca ttgattgcca
     5401 agtcaaaacg tcggataaca ccgtcacagt caagacagcg gtttaaaaat gaataccaat
     5461 ctctgttctg tgcctgcaag acatattcca tgttgcgtga gcctgcccct tttaattcca
     5521 agaatacgcc catgtgttcg tcatcagaag ccatgacctt gatttctccg taggcgtaat
     5581 gctctttata gccataatac ccatagtcat aatggataaa ataatctgct ttcatgccaa
     5641 gcacctttcg gataatttcc aatgcgtcag tcgttggata acggacagac agataatcaa
     5701 cgagcagata aaaaggctta tcgcagatat aataattcag acaacgcagg ataatatttt
     5761 gtgtttcctt gctggcgttg gcttttccgt tctcaaagcg gttgatacta tatcttgata
     5821 catgggtaag acttgcaagt tctccttgtg aaagaccgct tgcaagacgt ttgttcttca
     5881 tttcaagtgc aaattctaaa ttgttcattg tattcctcca atcccacaat agccttgaaa
     5941 tacctgtaaa cctctattca aaaagtgtga tatgcactat cttcttaaaa cttctcatta
     6001 ctactcacaa ctttgggatt gtatggtttt tgacttgtct tttgtgaaat agtgcccccc
     6061 tgttagataa cgggggcaca gacaaggctc gcaggctcgc cagccgacaa agcatttctt
     6121 ccccttgaca caaggtgtca tggcggtaag cctgagaggc ttccgccacc gtgtcagaaa
     6181 tactttgtca gcaagttctg ttttgccttt tctaacaagt tccatttcaa ccgatattca
     6241 tatctacgga tttaatcaga taggtatttc tttttgaatg tttttatatt tcattctatt
     6301 gtgcttattc gattacaaga acatagtaat tcttatatgc ttatttgtca atatcttttt
     6361 ttgcctttac aaatcatatt aagcatgata gacttataat agcgaagata taacgagata
     6421 tggagggtta tgcaatgaaa ataagccatt taggaaataa tatacagacc ataagaaaat
     6481 ttaggggaat gaaacaacag gaacttgcgg acaaaatcgg tatcaatatg cagagccttt
     6541 ccaagattga aagagggttg aactatcctg cctatgaaac gctggaaaag ataatggaag
     6601 tgctggacgt tacgcccaat gaattgttgt cgggagaatg gaagtatgtc aatcaatctg
     6661 aaaaggaagt ctgtcagttt ttaagagccg aagaacgtct aaatgcagaa ctgaaacatg
     6721 gacactatga taacttcttt gacagcgagg aagaatggct ggaatatgag ttagaaaagt
     6781 tacgggaata tattacagac tatatcaacg ggaaaagcat tgaagcgtcc gacctttacc
     6841 caatcaaaga atttatccag catttgaagt ttcaaaaatt attagaccgc tatgatgatt
     6901 tatacagtat ggatatgttt ggggaaagca tagagggaca taagtataag acaccttatc
     6961 aagtcgtgaa aatgataaac ccgaactcaa aagaagatat ggaactgtta cgagaagtat
     7021 taaggaataa caattttgat gatgaggacg agtaactttt ttcctcttgc gagaagatac
     7081 agcaaacaaa aagcccgatc aagagtgcaa agcaccacca atggtggcaa agctcttgac
     7141 agggcttttt cttttctgtt gtgggcaatc aagaggaaga aagggataaa atctgatttg
     7201 tggtaaatca ggcaaaggca acgcaggtat ctcaatttga tttggtactt gaaaagaacg
     7261 ggagtaattg gaagattata gaataacaaa tattggtaca ttattacagc tattttgtaa
     7321 tcacgtactc tctttgataa aaaattggag attcctttac aaatatgctc ttacgtgcta
     7381 ttatttaagt atctatttaa aaggagttaa taaatatgcg gcaaggtatt cttaaataaa
     7441 ctgtcaattt gatagtggga acaaataatt ggatgtcctt ttttaggagg gcttagtttt
     7501 ttgtacccag tttaagaata cctttatcat gtgattctaa agtatccgga gaatatctgt
     7561 atgctttgta tgcctatggt tatgcataaa aatcccagtg ataaaagtat ttatcactgg
     7621 gatttttatg cccttttggg tttttgaatg gaggaaaatc acatgaaaat tattaatatt
     7681 ggagttttag ctcatgttga tgcaggaaaa actaccttaa cagaaagctt attatataac
     7741 agtggagcga ttacagaatt aggaagcgtg gacaaaggta caacgaggac ggataatacg
     7801 cttttagaac gtcagagagg aattacaatt cagacaggaa taacctcttt tcagtgggaa
     7861 aatacgaagg tgaacatcat agacacgcca ggacatatgg atttcttagc agaagtatat
     7921 cgttcattat cagttttaga tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa
     7981 gcacaaactc gtatattatt tcatgcactt aggaaaatgg ggattcccac aatctttttt
     8041 atcaataaga ttgaccaaaa tggaattgat ttatcaacgg tttatcagga tattaaagag
     8101 aaactttctg ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg
     8161 acgaacttta ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta
     8221 gagaaatata tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata
     8281 agatttcaga attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg
     8341 attgataacc ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg
     8401 tctgaacttt gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca
     8461 tatatacgcc tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa
     8521 gaaaaaataa aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat
     8581 agagcttatt ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt
     8641 cttggagata caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta
     8701 caaacaactg ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg
     8761 gaaatctcag atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt
     8821 atactttctt tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag
     8881 tatcatgtgg agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa
     8941 aatgcagaat ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt
     9001 ttatctgtat caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt
     9061 ggatacttaa atcaatcgtt tcaaaatgca gttatggagg ggatacgcta tggctgtgaa
     9121 caaggattgt atggttggaa tgtgacggac tgtaaaatct gttttaagta tggcttatac
     9181 tatagccctg ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa
     9241 gtcttaaaaa aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg
     9301 ccacaggaat atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta
     9361 gacactcaat tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt
     9421 caagaatatc gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag
     9481 ttaaaagggt accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt
     9541 cggatagata aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat
     9601 ataaatatgg tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag
     9661 tgtaaaggag gagatagtta ttataaacta caagtggata ttgtgtcctg tatgtggaaa
     9721 taaaacacga ttaaagataa gggaagatac tgaattaaaa aaattccccc tctattgtcc
     9781 gaaatgcaga caagaaaatt taattgaaat aaagcagttc aaagtaactg tgattacaga
     9841 gccagacgca aagacgcaga gccgataaaa tgagattaat acaatctcat tttatcggct
     9901 ctttccgtta tgtatggatt cttttaatta gtcttcgatg tttcttgctt cgttgatacc
     9961 gctggctaaa gattccatta aggatagttc tttgtctgta aagctatcca tgtatttctc
    10021 tatctgtaat cgtcgggtgc tttttaccaa gttattagca ggtaagaaaa attcatcaac
    10081 ggaaacatga agtaacgata caaggtcata aagaacttgt atgctggggt gttgcccttt
    10141 attttcaata ttagttaagt accgtgggtc aatttcaatc aatgctccca cttgttcacg
    10201 agttaaacct cgtttcaatc gagcttcttt aatggctaaa ccaaaggctc taaaatcata
    10261 tttatcttct tttttacgca tagtagacca cctctataca ttttattgtt cctactgaat
    10321 taaaaacagg tatagaaaaa cgtgttatat ggtttatagg tttatattta ataaaaagca
    10381 ctactaaacg ccaataaaaa aaaccgttat atggtagtgc tatttacgct gttaaaatat
    10441 tgtatattac ttccaaatgg cggtttgttg gaggtcaacg tcgccatgaa gtacatcata
    10501 tacaataaat ttccttacat tgggttcttg tcaaaaaaag tcgtctatct gcaatagata
    10561 agtacgtcca ccaatgtggt tttataaatc atatagatag aataacagaa gcatgtaaac
    10621 agagaaataa atctgtttat atgctttttt ggctattcag aactttttta caaagtttat
    10681 ttatcagtaa tgcaacaaat ccccctttca cattgggact aagagtgaaa ggagataaac
    10741 gagcaaggct cacttccttt cctagacaga aagggggtga gattatgagg acatcttctt
    10801 tcgagcatat cgtcagatta cagttcaatt ctttaatgct gattgtcatt aagaacaaag
    10861 tgaaaagtcg aaacagacag cttgcaagac attctaaacg tgaagtattg ttttgtgaaa
    10921 tagccgaaac gaaacaagtt gaacgtggaa cgattgatac ctgtttttat gattgtgtat
    10981 catttaaagt cttgcacttt acaatttatg tatctgatga aacacttggt acagttttac
    11041 atagactatc agaaaaacag cgtagtgcga ttttattacg ttattttca
//
LOCUS       DASJUF010000322         8062 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.116, whole genome shotgun sequence.
ACCESSION   DASJUF010000322 DASJUF010000000
VERSION     DASJUF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.116"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..1564
                     /locus_tag="U4B62_003103"
     CDS             128..1564
                     /locus_tag="U4B62_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2143158.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="U4B62_003104"
     CDS             1753..4200
                     /locus_tag="U4B62_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN2143159.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="U4B62_003105"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="U4B62_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN2143160.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="U4B62_003106"
     CDS             complement(5690..6793)
                     /locus_tag="U4B62_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2143161.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="U4B62_003107"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="U4B62_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2143162.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DASJUF010000323         5905 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.140, whole genome shotgun sequence.
ACCESSION   DASJUF010000323 DASJUF010000000
VERSION     DASJUF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.140"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            424..888
                     /locus_tag="U4B62_003108"
     CDS             424..888
                     /locus_tag="U4B62_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2143163.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            872..1501
                     /locus_tag="U4B62_003109"
     CDS             872..1501
                     /locus_tag="U4B62_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2143164.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1855..3333
                     /gene="msr(C)"
                     /locus_tag="U4B62_003110"
     CDS             1855..3333
                     /gene="msr(C)"
                     /locus_tag="U4B62_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2143165.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3380..3826
                     /locus_tag="U4B62_003111"
     CDS             3380..3826
                     /locus_tag="U4B62_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN2143166.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4025..4540)
                     /locus_tag="U4B62_003112"
     CDS             complement(4025..4540)
                     /locus_tag="U4B62_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2143167.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4657..5859
                     /locus_tag="U4B62_003113"
     CDS             4657..5859
                     /locus_tag="U4B62_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2143168.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1824 a          983 c         1246 g         1852 t
ORIGIN      
        1 tcatgttttt tgtttggttg aacttattac cgcagatgca agtttttgaa caccgtttac
       61 tcaaaaaagg ttgtgacaac atcttgtcat aactttttta ttactgttta agcatactga
      121 tccaatttct aatattcgac gggattcttg caaaaggcga tttcggaaat ttttggcggt
      181 gtaaaattat ctactgcaga agagctatct tctatagttt gataagaatc aataatcttg
      241 gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa atttcttttc
      301 tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt gacttttttc
      361 tttttttatt ctattattga caagtcaatc gttgacgagt caattattag aaaggaggaa
      421 attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc cgatcaaaaa
      481 atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga gatcatgatg
      541 agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt gcagattgat
      601 caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt ttcgcgtcag
      661 agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc ccagaagaaa
      721 atcaagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc ggctttcagc
      781 gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca actttgcaat
      841 ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt tttcaacaat
      901 tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga
      961 aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata tgaaaccatg
     1021 gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt
     1081 caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg atatgcaatg
     1141 ttacgaatat ggcgaagaaa tctacaacca agcttacgaa tcagggaaga tgccaaaaga
     1201 agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt caagagaaga
     1261 aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca tgttagttgc
     1321 gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc agttggcaga
     1381 ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa taggaaaagc
     1441 ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta tgttcgaata
     1501 aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg tggtaaccta
     1561 ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga acttttgaat
     1621 aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg gagtatgtcc
     1681 attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga tttagtcata
     1741 gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca gatttttctg
     1801 ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa acatatggaa
     1861 aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct agaactatct
     1921 attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga aaatggtgtc
     1981 ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca tgggaaaatc
     2041 caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga agcaaaggac
     2101 ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc agagcggaaa
     2161 tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa
     2221 gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga tcgactgatt
     2281 gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg ctattttcta
     2341 gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga gttttcgggg
     2401 aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct acgagaagca
     2461 gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa
     2521 gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag actggatcgg
     2581 ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca
     2641 ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat gaaacaaatc
     2701 cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat gggacaaaat
     2761 gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca gttttcttta
     2821 ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt attagaacat
     2881 atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca agtatatcag
     2941 caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat gagacaaaca
     3001 gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc tcaggaaact
     3061 ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac gattgctttg
     3121 ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt tattgatatg
     3181 gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat tttgtttact
     3241 tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat aaaagggcag
     3301 aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt tcgtgacaaa
     3361 tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt ttacaacaat
     3421 gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc atcaagatct
     3481 tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt tcattttgga
     3541 tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag taaatccaga
     3601 aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa tggtgacatg
     3661 tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat tctctgctta
     3721 tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga tcatcgttgg
     3781 cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga acactgttat
     3841 ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt ctagaaaaat
     3901 agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt tccaactgta
     3961 ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag gttctgttta
     4021 tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat tggagttcct
     4081 gctgacaaag cataattctg cgctggatca caacataacg tccaaattct tgtttcttgt
     4141 ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc agctgttgtt
     4201 ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt
     4261 ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc cattcctcta
     4321 caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt
     4381 ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag atctgactgt
     4441 gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa cgtggtttgg
     4501 ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta cctctcgtct
     4561 tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata ttgacatgta
     4621 aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga gaatcaaata
     4681 gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct agttgtagca
     4741 gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt aaaaacgatt
     4801 gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag ttacatgtta
     4861 gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg tttgaaaaaa
     4921 acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg attatctggg
     4981 aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc
     5041 tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa agcaatcatg
     5101 atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg
     5161 ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt ttgcggtttt
     5221 atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt aggcctttgg
     5281 gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat cgcattgtta
     5341 tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat tggctattct
     5401 gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat tgcttcgatt
     5461 ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt agcagcattt
     5521 gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc agtccttgta
     5581 ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact gttgacgacg
     5641 attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta tagttttgtc
     5701 cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga acatattggt
     5761 gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt
     5821 tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa
     5881 gctgtcctgc atcaagtcat aagaa
//
LOCUS       DASJUF010000324         5783 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.146, whole genome shotgun sequence.
ACCESSION   DASJUF010000324 DASJUF010000000
VERSION     DASJUF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.146"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..981
                     /locus_tag="U4B62_003114"
     CDS             85..981
                     /locus_tag="U4B62_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN2143169.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1131..2072)
                     /locus_tag="U4B62_003115"
     CDS             complement(1131..2072)
                     /locus_tag="U4B62_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2143170.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2198..3319
                     /locus_tag="U4B62_003116"
     CDS             2198..3319
                     /locus_tag="U4B62_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2143171.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3577..5079
                     /gene="eat(A)"
                     /locus_tag="U4B62_003117"
     CDS             3577..5079
                     /gene="eat(A)"
                     /locus_tag="U4B62_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2143172.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5107..5673
                     /locus_tag="U4B62_003118"
     CDS             5107..5673
                     /locus_tag="U4B62_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2143173.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1892 a         1025 c         1220 g         1646 t
ORIGIN      
        1 aaatatagga aacgttttac tggcttttta ctatttaaac aggctatcat aggaaaaagt
       61 gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg tactgctagt tgcaaaaaat
      121 aaatcacttt attttttgcc ttccatccgc tctttagatg tttggtccgg aaaagattta
      181 ctacagtcgg aattcattaa atcagctaga caacagttgg caaattatca gctgatcgtt
      241 tttttagatt atggatttga tatggagatg gctcgacgcg tgcgcccatt taccaatgca
      301 aaaattgtgt tatttttttg gaatcacttc aaagcagaac acgaggcgat gctgaagcag
      361 gcacaagatg aaccggcaat cgatgagatt tatcattttg attttttgga agcaaaagaa
      421 cttgggctga aacataatag ttcattttat tcgaaacata tgaatttttc ttctagcgaa
      481 ccaaagacgg atctattttt tggtgcaacg aataatggga gaaaagaacg agcagaaagc
      541 tataagcagg aatttataaa aagagatgtt acgaccaact atttcattct tccacatcga
      601 gggaatgaac aacctggata tcttacttat gaagagtacc tgaaaaaaac aggggaatcg
      661 cgcgggattc tggaactgtt gcgggagggt cagcaaggag tgactttgcg gacatttgaa
      721 tccatgtatt ttcaaaagaa actagtgacg gataatcaag cgattgctca ttatcatttt
      781 tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt tagatgaatt accggaattt
      841 ctgcatacac catttaagcc aatcctatca gagctattgg aattttatga tgcagaaaat
      901 tgggcaaaac gatttatcac taacgattcc actatttatg agcaatacga atatgacctc
      961 tcctctgttg aaaacaaata aaaaacctac attttgaaga gattatgtac tgaaacaaac
     1021 aagcacaatt cttatggcat catgagagtt gtgttttctt tttgatcttt tattgggctt
     1081 aaaaaaagag aaccagctgc tgattcagct gattctcatc agagagcctt ttattctgcg
     1141 ccaaggattc cttcttttgc atggactaat tgggtcaaaa aggcacaata tggtgtagca
     1201 atatcatatt tttgtccttt tcgccaaatg gcaccattga tgtagtcgat ttcagtcaaa
     1261 cgatggtttt tgatcaggtc ttggtacata gatgggtaat gcaacccgat gttgtcagga
     1321 tcataagtag aagcaatatg ttggtacact tcttcttgat ctagtatgat tccttctttt
     1381 gctgcgatag aagcaaattc agatacgatt gttcgaacca tgctctctgc cgctttttgc
     1441 gcgcctagtt cagcaatatt acaatccaaa atcgtgcata aaccattcaa tgttccattt
     1501 acacaagctt ttcgccaaat ggaataacga acgttatggc tatagatcgg atttaaattt
     1561 gcttcttgaa agacttctac aacttttttt gtaaatgctt cgccttctgg ttccaagttc
     1621 tctagttcga tttctccatc acctaacaat gtgacttttc cgggaccagc tagaccagct
     1681 gtccacatcg tgatcccaaa taagatattt ttctttggca catatttttc cagtacatct
     1741 tcatgaccta atccatttag taaacacaaa acataagtat tgtcagtaat aatggattgg
     1801 atcgaacaaa acatatcatc taattgattg gcttttgtta atgctacgat caagtccacg
     1861 tgttcgttgc tttcgatgat ttcttctggt gaatagattg gcagttttgc gaccacttct
     1921 ttaccgttaa agtcagcaat caacccattt gtacgaatgg cttcaatgtg agcaggccaa
     1981 cgatcgatca agaggacctc atttccgctt tgatgtagca tcaatccgat tcggctgccc
     2041 attgctccgg ccccagctat tgcgattttc atgttttttc tctcctttat tcaatgagtt
     2101 cccttttagt atatagaaaa atggaagaaa atagcattgt tttgtttgaa aaaaatgaaa
     2161 aaaagtgtct tacttaagaa gaaagtgagg gatacagatg gatattttgg cggcaattga
     2221 ttcatttaaa ggaagtgctt cttcatttga actaaatcag gcagttctaa atggctggga
     2281 agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa atactgcaga
     2341 agcaatctat tacgcgctca ggggaacatg gcgtaccatt gatgggatgg atctcttatt
     2401 ccgtcagcga aaaatcagct atttattgac aacttggcaa ggaaaaaaca ccgcagtcat
     2461 cgaatctgca caagcattgg ggttagatct acttgataaa ccaacggatc aaactatccg
     2521 acttgcttcc agttatgcct taggagaact tattaaagat gcactgcttc aagaagtaga
     2581 acaaattatt gtcactttag gaggaagcgg gtgtagcgat ggtggtctag gattactcca
     2641 atccttgggt gcttctttag aagggtacac agaaggaaat cctttattat ctactaaaaa
     2701 acttcatttt gatcgaagaa acgttcttgt ttctaaagta aaattgatta tcgctgcaga
     2761 tgttaccaat ccatatacag gtcaacaagg agctgcttgg caatttggaa gacaaaaagg
     2821 aggaatagat acgacgctag ctttcttaga taaacaagca ggaaagattg ctcagcaaat
     2881 aaaagatttc tgggaggtgg atttagatga tattccagga agtggagcag caggcggtat
     2941 tggtggagcc ctttttttgt tgggagcgaa gatggtttct ggtttcgatt tagtcagcca
     3001 aatcactgga ttggaaaaaa agatcgaaca agcagatgtc atcattacag gagaagggcg
     3061 gatcgatcag caaacgatcc acggaaaagt tccatacggt gtagcagtgc ttgcaaaaaa
     3121 atatcgaaaa ccaataatcg ctatttgcgg aagtagagac caagagctag aaggactttc
     3181 tgattttctc ccaattgtct taagtattca tttgggtcca ataggtttag aagaagcaat
     3241 ggaacacagg caaacattag aaaaaacaaa aatcttagga cagacactta gcagattttt
     3301 caccatcctc ccgaagtagt tgacaaaatc attttcttta tgctatcgta atttcaaata
     3361 taggacaaag tgagggatcc gtgcattgaa aaattaagtc gattgatgaa agctgcattc
     3421 agactgtacc ttgtggagac aaggaagcag tgtgcagatt ttcgggattg gcttggtttt
     3481 ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca tcctctttag ccagttctga
     3541 aaataggact ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat
     3601 ctgacattcg gctacgacag ccaaggcaca ttattatttg aacaagcaaa tctaaatttt
     3661 gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg
     3721 aatattctac aaaacaaact accttatcaa gggcaagtaa tccatcagca agaatttgcc
     3781 tatttcccgc aacagacaaa agataaagaa cgtttaacct attacgtgtt aaatgatatt
     3841 acggattttg agatatggga aatcgaaaga gagctccaat tgatgcaaac agatcctgaa
     3901 atcttatgga gagaattcag cacactatcg gggggagaga agacaaaagt cctactggca
     3961 cttttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttggat
     4021 atctctggta gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg
     4081 gtcagccatg accggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa
     4141 agtcaactgg aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt
     4201 gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa
     4261 acagcagctg aaaaagccga atggtctcgt tcccgagaag gagataaaac aaagaaacaa
     4321 gtcggattca tcgatactga atctagacga gtgaataaag gagcagtggg tgctgatgct
     4381 gcacggacga tgaaacgatc caaagcaatc gtgaatcgga tggagaccca gatcagcgag
     4441 aaagaaaaac tattaaaaga tatcgaatat atcgattctt tgacgatgaa tagccaagcg
     4501 tctcaccata agcgactttt aagcgtagaa gatcttcaat tagggtatga aaatctgtta
     4561 ttcgagccaa ttcattttac aatcgagcct catcagcggg tggcgatttc aggtcctaac
     4621 ggtgcaggaa agtcatccat tatccattat cttctggggg cattcaacgg caaggttata
     4681 ggagaaaaat accagccaaa acatctgagc attagttatg caagccaaaa ttatgaagac
     4741 aatcgaggaa cgttggcgga atttgcagag aaaaaccaag tagactacca agcatttttg
     4801 aacaacctcc gaaagcttgg gatggaaaga gatgtttttc ataacaagat cgagcagatg
     4861 agtatgggcc aacggaaaaa agtggaattg gctaaatctt tatcacagcc agctgaacta
     4921 tatacatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa
     4981 ctgatcttga acgtgaaacc tgccatgtta ctagtggaac atgatcaaac cttcctggat
     5041 aaagtatcta ctgagattat ttctcttgag agaatctaac ccttgtaatc tcaagaacaa
     5101 caaggtatgg ttaaggggaa ctttgccaac gaaggagaga atcacatgct ttatgtaacg
     5161 agacacggag aaacaacttg gaacgcacaa ggattagttt gcggacgggc agatgtgccg
     5221 ctgactgaaa aaggacagat gcaggcacag aaactagcag aaaaggtagt agatttgcct
     5281 gttccaatca cgaaaatcat ccattcccct cttcaacgag caagagatac agcacaagca
     5341 gttgctgatc gtctcagctt gccgctgacc gttgatgaac gattggtaga aatggatttt
     5401 ggggattatg atggactgcc aagcaaagat gagaactttc aaaaagcacg tttagcgttc
     5461 gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat cgttccttta
     5521 ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca taatgccttg
     5581 ataagagtga tcaatgctta ttttcatccg atgcctaatg aggggttctt tacattcatg
     5641 gtagataata cagaattggt ttcttatgag taattatgtt tttgtgttat ttaaataaaa
     5701 tatttcaagg cactgctttt gcttagcagt gcctttttta ggttatgaca attcttttga
     5761 taatataaac tcaaccttta gaa
//
LOCUS       DASJUF010000325         5156 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.155, whole genome shotgun sequence.
ACCESSION   DASJUF010000325 DASJUF010000000
VERSION     DASJUF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.155"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            86..733
                     /locus_tag="U4B62_003119"
     CDS             86..733
                     /locus_tag="U4B62_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HEN2143174.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1426..2364)
                     /locus_tag="U4B62_003120"
                     /pseudo
     CDS             complement(1426..2364)
                     /locus_tag="U4B62_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2364..2981)
                     /locus_tag="U4B62_003121"
     CDS             complement(2364..2981)
                     /locus_tag="U4B62_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2143175.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(2995..3165)
                     /locus_tag="U4B62_003122"
     CDS             complement(2995..3165)
                     /locus_tag="U4B62_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143176.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3513..5003)
                     /locus_tag="U4B62_003123"
     CDS             complement(3513..5003)
                     /locus_tag="U4B62_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2143177.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
BASE COUNT         1585 a          896 c          798 g         1877 t
ORIGIN      
        1 atttacgagg tgacataacg tatgaaaaaa tcagaggatt attcctccta aatataaaaa
       61 tttaaaattt aggaggaagt tatatatgac ttttaatatt attgaattag aaaattggga
      121 tagaaaagaa tattttgaac actattttaa tcagcaaact acttatagca ttactaaaga
      181 aattgatatt actttgttta aagatatgat aaaaaagaaa ggatatgaaa tttatccctc
      241 tttaatttat gcaattatgg aagttgtaaa taaaaataaa gtgtttagaa caggaattaa
      301 tagtgagaat aaattaggtt attgggataa gttaaatcct ttgtatacag tttttaataa
      361 gcaaactgaa aaatttacta acatttggac tgaatctgat aaaaacttca tttcttttta
      421 taataattat aaaaatgact tgcttgaata taaagataaa gaagaaatgt ttcctaaaaa
      481 accgatacct gaaaacacca taccgatttc aatgattcct tggattgatt ttagttcatt
      541 taatttaaat attggtaaca atagcagctt tttattgcct attattacga taggtaaatt
      601 ttatagtgag aataataaaa tttatatacc agttgctctg caacttcatc attctgtatg
      661 tgatggttac catgcttcac tatttatgaa tgaatttcaa gatataattc atagggtaga
      721 tgattggatt tagtttttag attttgaaag tgaatttaat tttatacacg taagtgatca
      781 taaaatttat gaacgtataa caaccacatt ttttggttgc ttgtggtttt gattttgaat
      841 ttggttttga acttatggac tgatttattc agtccatttt ttgtgcttgc acaaaaacta
      901 gcctcgcaga gcacacgcat taatgactta tgaaacgtag taaataagtc tagtgtgtta
      961 tactttactt ggaagatgca ccgaataaaa aatattgaag aacaactagc aaaagatttt
     1021 aaagagttat tttattttaa gtctttataa catgagtgaa gcgaattttt aaatttcgat
     1081 agaaattttt acatcaaaaa gccccctgtc aaaattgacg aagggggttt tttggcgcac
     1141 gcttttcgtt agaaatatac aagattgaaa atcgtgtata agtgcgccct ttgttttgaa
     1201 cttagcacgt tacatcaatt ttttaaaatg atgtataagt gcgccctttt aaattttgag
     1261 tgattatatt ttttgagtta gaaaaaggga ttgggaaaat ttcccaaaat aatttaaaaa
     1321 ataagcaaaa attttcgata gagaatgtgc tattttttgt caaaggtgta taccttgact
     1381 gtgcttgctg ttacattaag tttattttta agttattaaa aaagaaatag cttttaaagt
     1441 ttggctcgct gtcgctttat aaagctgatt gacttttgat tgcaaactac ttaaagaaaa
     1501 caaactcgga ctattcgttt tcttctcttt ggtttgaaca tcagcaatta tcccctcttg
     1561 attgcctatt ttagcttgtt tagaagaaac aaaagctaaa agctcctctt gggttttaaa
     1621 acgctgtgtg gggcttagaa cgcccttaaa cgacccttgg tttactttta tactagcttc
     1681 cacctcgaaa aaaggttctt ttttaaaatt ctctatggct tcctggcgct gaaaaataag
     1741 gtataaggtg ggcgtttgaa cacgtcctag tgaaaatgta ccttgtacgc ccttctgttg
     1801 taaatttaac gtatacaaag ggcttgcgtt catgccgatc aaccaatcgg caatttggcg
     1861 tgtttgcgct tcttgataaa agggatagta attcattcca ggttgcaaat tttgaaaacc
     1921 gcttcggatt acatcttttt ctaagctatt gatccatagt cttttaaatg ttttatcttt
     1981 tgaaaaggca tttgctttat ggataatcga ccaggcgata ttttcacctt ctctgtcgct
     2041 atctgttgca acaataattg tatttgcctt tttgagaagt tctgcaacaa ttttaaactg
     2101 ctttccctta tcttttgcaa cttcaaaatc gtatcgatca ggaaaaatcg gcaaagattc
     2161 aagtttccaa ttttgccact tttcgtcata atgacctggt tctgctaatt ccactaaatg
     2221 cccaaaacca aaggtgataa acgtttcatc tgtaaatagt gggtctttga tctcaaaata
     2281 accgtctttt ttggtgcttt gttttaaagc acttgcgtag gctaatgcct ggcttggttt
     2341 ttcagctaaa ataaccgtac tcattaacta tccctctttt cattgttttt tctttgatcg
     2401 actgtcacgt tatatcttgc tcgatacctt ctaaacgttc ggcgattgat tccagtttgt
     2461 tcttcaactt ctttatcgga taaaccattc aaaaacaaat cgaaagcatg ctttaaacgt
     2521 ggatcatttt ctttaaattt atgctgacga cctttgaatt tgcctttttt cttagcaatt
     2581 tcgattcctt gtgcctgacg ttccttaatt ttttttcgtt ctgattctgc ttgatacttg
     2641 tacaattcaa tgacaaggct attaatcaaa cgccttaaat tttcatcttc aataccattc
     2701 attgagggta aatttaagac ttccagggtt gcccccttaa tttgaatttg attcatcaat
     2761 tctgttaatt ctttattatt tcgtcctaat cgatctaatt cagtaacaat aacaatatcc
     2821 ccttcacgaa tatagttaag catagcttgt aattgtgggc gttcgaccga ttgaccgctt
     2881 aatttgtctg aaaagacctt agaaacgccc tgtaacgctt gtaattgccg atctaagttc
     2941 tgttctttgc tactgacacg tgcataacca attttagcca ttttcaacca acctctaaaa
     3001 ttctctcggt tgcaataacc aatcagcaat atctactttt tcaatttcaa attgcttatc
     3061 agaaattgtc ttttcgtaag cgataaaatc ttgcgcatat tgttgctcat taaaaatagc
     3121 caccacttcg tcattttcta aaactcgata aataaatttt ttcattttac tcctcctatt
     3181 atgcccaact taaatgacct attcaccaag tcaattatac tgctaaaatc atattaggac
     3241 aaataggtat actctaatga cctataaaag atagaaaatt aaaaaatcaa gtgttcgctt
     3301 cgctctcact gcccctcgac gttttagtag cctttccctc acttcgttca gtccaagcca
     3361 actaaaagtt ttcgggctac tctctccttc tccccctaat aattaattaa aatcttactc
     3421 tgtatatttc tgctaatcat tcgctaaaca gcaaagaaaa aacaaacacg tatcatagat
     3481 ataaatgtaa tggcatagtg cgggttttat tttcagcctg tatcatagct aaacaaatcg
     3541 agttgtgtgt ccgttttagg gcgttctgct agcttgttta aagtctcttg aatgaatgta
     3601 tgctctaagt caaaagaatt tgtcagcgcc tttatatagc tttctttttc ttcttttttt
     3661 actttaatga tcgatagcaa caatgattta acactagcaa gttgaatgcc accatttctt
     3721 cctggtttaa tcttaaagaa aatttcctga ttcgccttca gtaccttcag caatttatct
     3781 aatgtccgtt caggaatgcc tagcacttct ctaatctctt ttttggtcgt cactaaataa
     3841 ggcttgtata catcgctttt ttcgctaata taagccatta aatcttcttt ccattctgac
     3901 aaatgaacac gttgacgttc gcttcttttt ttcttgaatt taaaccaccc ttgacggaca
     3961 aataaatctt tactggttaa atcacttgat acccaagctt tgcaaagaat ggtaatgtat
     4021 tccctattag ccccttgata gttttctgaa taggcacttc taacaatttt gattacttct
     4081 ttttcttcta agggttgatc taatcgatta ttaaactcaa acatattata ttcgcacgtt
     4141 tcgattgaat agcctgaact aaagtaggct aaagagaggg taaacatgac gttattacgc
     4201 cctattaaac ccttttctcc tgaaaatttc gtttcgtgca ataagagatt aaaccagggt
     4261 tcatctactt gttttttgcc ttctgtaccg cttaaaaccg ttagacttga acgagtaaag
     4321 cccttattat ctgtttgttt gaaagaccaa tcttgccatt ctttgaaaga ataacggtaa
     4381 ttaggatcaa aaaattctac attgtccgtt cttggtatgc gagcaatacc aaaatgatta
     4441 cacgttagat caactggcaa agactttcca aaatattctc ggatattttg cgaaattatt
     4501 ttggctgctt tgacagattt aaattctgat tttgaagtca catagactgg cgtttctaaa
     4561 acaaaatatg cttgataacc tttatcagat ttgataatca tagtaggcat aaaacctaaa
     4621 tcaatagcgg ttgttaaaat atcgcttgct gaaatagttt cttttgccgt gtgaatatca
     4681 aaatcaataa agaaggtatt gatttgtctt aaattgtttt cagaatgtcc tttcgtgtat
     4741 gaacggtttt cgtctgcata cgttccataa cgataaacgt tgggtgtcca atgtgtaaat
     4801 gtatcttgat tttcttgaat cgcttcctcg gaagtcagaa caacaccacg accgccaatc
     4861 atgcttgatt ttgagcgata cgcaaaaata gcccctttgc ttttacctgg cttggtagtg
     4921 attgagcgaa ttttactatt tttaaatttg tactttaaca agccgtcatg aagcacagtt
     4981 tctacaacaa aagggatatt cattcagctg ttctcctttc ctataaatcc tataaaatag
     5041 gttgtttaat taacttggtt tgctttttca ttcaactgtt tcaatattgc atgttttgaa
     5101 aaagattttt ttcctttata agtcaatttt tttccactaa tcgaataaat tatttt
//
LOCUS       DASJUF010000326         4802 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.163, whole genome shotgun sequence.
ACCESSION   DASJUF010000326 DASJUF010000000
VERSION     DASJUF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.163"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            90..785
                     /gene="vanR"
                     /locus_tag="U4B62_003124"
     CDS             90..785
                     /gene="vanR"
                     /locus_tag="U4B62_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN2143178.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="U4B62_003125"
     CDS             763..1917
                     /locus_tag="U4B62_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2143179.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="U4B62_003126"
     CDS             2132..3100
                     /locus_tag="U4B62_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN2143180.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="U4B62_003127"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="U4B62_003127"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN2143181.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="U4B62_003128"
     CDS             4130..4738
                     /locus_tag="U4B62_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEN2143182.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DASJUF010000327         4227 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.179, whole genome shotgun sequence.
ACCESSION   DASJUF010000327 DASJUF010000000
VERSION     DASJUF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.179"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            322..1014
                     /locus_tag="U4B62_003129"
     CDS             322..1014
                     /locus_tag="U4B62_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2143183.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="U4B62_003130"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="U4B62_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN2143184.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="U4B62_003131"
     CDS             complement(1910..3862)
                     /locus_tag="U4B62_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2143185.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="U4B62_003132"
     CDS             complement(3869..4105)
                     /locus_tag="U4B62_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143186.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1428 a          699 c          704 g         1396 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgt
//
LOCUS       DASJUF010000328         3206 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.198, whole genome shotgun sequence.
ACCESSION   DASJUF010000328 DASJUF010000000
VERSION     DASJUF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.198"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            260..1171
                     /locus_tag="U4B62_003133"
     CDS             260..1171
                     /locus_tag="U4B62_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN2143187.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ"
                     /locus_tag="U4B62_003134"
     CDS             1324..1809
                     /gene="vanZ"
                     /locus_tag="U4B62_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEN2143188.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="U4B62_003135"
     CDS             2328..2612
                     /locus_tag="U4B62_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2143189.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2978..>3206
                     /locus_tag="U4B62_003136"
     CDS             2978..>3206
                     /locus_tag="U4B62_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143190.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTY"
BASE COUNT         1197 a          449 c          588 g          972 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc
     3181 gattagtgga aaaaaattga cttata
//
LOCUS       DASJUF010000329         2690 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.207, whole genome shotgun sequence.
ACCESSION   DASJUF010000329 DASJUF010000000
VERSION     DASJUF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.207"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..484
                     /locus_tag="U4B62_003137"
     CDS             <1..484
                     /locus_tag="U4B62_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN2143191.1"
                     /translation="SAMYRGELAISKILYAKNESLCELKKQAEIYPTALKKSLMNFFI
                     FEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETF
                     EYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
                     "
     gene            465..1199
                     /locus_tag="U4B62_003138"
     CDS             465..1199
                     /locus_tag="U4B62_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2143192.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1232..2140
                     /locus_tag="U4B62_003139"
     CDS             1232..2140
                     /locus_tag="U4B62_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN2143193.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2137..2617
                     /locus_tag="U4B62_003140"
                     /pseudo
     CDS             2137..2617
                     /locus_tag="U4B62_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          945 a          376 c          587 g          782 t
ORIGIN      
        1 aagtgcaatg tatcgtggag aattagcgat tagtaaaata ctatatgcta agaacgaaag
       61 cttatgcgaa ttaaaaaagc aggcagaaat ttaccctact gctctaaaga aaagcttgat
      121 gaactttttt atatttgaag ctgaattctc tttaatgttt gtaaaagcaa atgcgggggt
      181 agaggataaa tattatattg caggccatgt ttttcgtatt atttcatgct taaatcaagt
      241 actatttgca tgtaataatg cttattgcat taacgaaaag aaagctataa aactgcttga
      301 aacttttgaa tataaacctg aaaaatatgc cgaaagagtt aatcatattt ttgaagtact
      361 cggtctttct ctttttgaat gctacgatat gaccgagaaa ctttataaag aagtgaatga
      421 aattgtatcg gagataaata actttttaaa cgaggagagt tcagatgaaa gaaaacaaat
      481 atgatgataa tatatttttt caaaaataca gtcaaatgag tcgctcgcag aaaggactgg
      541 ctggtgcggg agaatgggag actttgaaaa agatgctacc tgattttaag ggtaagcgtg
      601 tgcttgattt aggatgcggc tatggatggc actgtatata tgcgatggaa aacggtgctt
      661 cctctgtagt aggtgttgat atttctcata aaatgctcga agtagcaaaa ggaaaaaccc
      721 attttccaca gattgaatat gaatgctgtg ccatagaaga tgtggatttc ccagaggaga
      781 gctttgatgt aatactaagt tcgcttgcgt ttcattatgt agcagactat gagaatttaa
      841 taaaaaagat atataggatg ctgaaggctg gtggcaattt agtttttaca gttgaacatc
      901 ctgtttttac tgctcatgga acacaagact ggtattataa cgaaaaagga gaaatactgc
      961 atttcccggt ggacaattat tattatgagg gcaaacggac agctatgttt ttggaagaaa
     1021 aggttacaaa atatcataga acactgacca catatctaaa tacactgctt tcaaatagtt
     1081 ttataataaa tcagattgtg gagccacagc cgccagagaa catgatggat attccgggga
     1141 tggcggatga aatgcgacgc ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat
     1201 aatatagaaa aaataaacga ggagtatgta aatgagatca gaaaaagaaa tgatggattt
     1261 agtactttct ttagcagaac aggatgaacg tattcgaatt gtgacccttg aggggtcacg
     1321 cgcaaatatt aatataccta aagatgaatt tcaggattat gatattacat attttgtaag
     1381 tgatatagaa ccgtttatat ctaatgatga ctggcttaat caatttggga atataataat
     1441 gatgcaaaag ccggaggata tggaattatt cccacctgaa gaaaagggat tttcctatct
     1501 tatgctattt gatgattaca ataaaattga tcttacctta ttgcccttgg aagagttaga
     1561 taattaccta aagggcgata aattaataaa ggttctaatt gataaagatt gtagaattaa
     1621 aagggacata gttccgactg atatagatta tcatgtaaga aagccaagcg caagggagta
     1681 tgatgattgc tgcaatgaat tttggaatgt aacaccttat gttattaaag gattgtgccg
     1741 taaggaaatt ttatttgcta ttgatcattt taatcagatt gttcgccatg agctgctgag
     1801 aatgatatca tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta
     1861 taagtttatt gaaaggtata tatccgagga tttgtgggag aaacttttgt ccacctaccg
     1921 gatggattcc tatgaaaaca tatgggaagc attatttcta tgccatcaat tgttcagggc
     1981 ggtatccggt gaggtggcgg aaaggcttca ttatgcctat ccggagtatg ataggaatat
     2041 aacaaaatat accagggaca tgtataaaaa atacactggt aaaaccggct gcctggatag
     2101 cacatatgcc gctgatatag aagagaggcg ggaacagtga ttacagaaat gaaagcaggg
     2161 cacctgaaag atatcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg
     2221 aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa
     2281 agctaccaag acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac
     2341 aatactgata agataatata tcttttatat agaagatatc gccgtatgta aggatttcag
     2401 ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa
     2461 cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca
     2521 taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     2581 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     2641 gttcgtcttg tggttctgtt gcaaagtttt aaataaagaa taaaatccct
//
LOCUS       DASJUF010000330         1651 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.236, whole genome shotgun sequence.
ACCESSION   DASJUF010000330 DASJUF010000000
VERSION     DASJUF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.236"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..341)
                     /locus_tag="U4B62_003141"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="U4B62_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1055..1345)
                     /locus_tag="U4B62_003142"
     CDS             complement(1055..1345)
                     /locus_tag="U4B62_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2143194.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          537 a          310 c          229 g          575 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag
     1561 tatcaaattg caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta
     1621 atttacacca caaaatgtaa gttaaaatgt a
//
LOCUS       DASJUF010000331         1107 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE2220
            SAMN38756207-rid20573293.guided.272, whole genome shotgun sequence.
ACCESSION   DASJUF010000331 DASJUF010000000
VERSION     DASJUF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756207
            Sequence Read Archive: SRR27167943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028109.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:20:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,004
            CDSs (with protein)               :: 3,004
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 34 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 16 of 79
            Pseudo Genes (multiple problems)  :: 9 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756207-rid20573293.guided.272"
                     /isolate="VE2220"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2020-12-16"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            160..897
                     /gene="erm(B)"
                     /locus_tag="U4B62_003143"
     CDS             160..897
                     /gene="erm(B)"
                     /locus_tag="U4B62_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN2143195.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          410 a          192 c          180 g          325 t
ORIGIN      
        1 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
       61 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      121 gtatttgttt atagatgtaa tcacttcagg agagattaca tgaacaaaaa tataaaatat
      181 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      241 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      301 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      361 tcagaaaaat taaaactgaa tactcgtgtc actttaattc accaagatat tctacagttt
      421 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      481 caaattatta aaaaagtggt ttttgaaagc cgtgcgtctg acatctatct gattgttgaa
      541 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      601 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      661 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      721 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      781 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccattact
      841 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
      901 tatgagtcgc ttttttaaat ttggaaagtt acacgttact aaagggaatg gagataaatt
      961 attagatata ctactgacag cttccaagaa gctaaagagg tccctagcgc ctacggggaa
     1021 tttgtatcga taaggggtac aaattcccac taagcgctcg ggaccccttg taggaaaatg
     1081 tcctaagtgt ggcaacaata ttgtatt
//
